Patent ID: 8679767

Claim:
A method of detecting human or humanized antibodies comprising the steps of: (a) treating a biological sample comprising a human or humanized antibody with a digestive enzyme to form a digested antibody sample, wherein the biological sample is serum, plasma, tissue, or cells from an animal selected from a cynomolgus monkey, a rat and a mouse that has been treated with said human or humanized antibody; and (b) analyzing the digested antibody sample by mass spectrometry to detect one or more human framework peptides, wherein the human framework peptides comprise one or more sequences selected from SEQ ID NOS. 1-8: GPSVFPLAPSSK SEQ ID NO: 1 STSGGTAALGCLVK SEQ ID NO: 2 TPEVTCVVVDVSHEDPEVK SEQ ID NO: 3 FNWYVDGVEVHNAK SEQ ID NO: 4 VVSVLTVLHQDWLNGK SEQ ID NO: 5 ALPAPIEK SEQ ID NO: 6 GFYPSDIAVEWESNGQPENNYK SEQ ID NO: 7 TTPPVLDSDGSFFLYSK. SEQ ID NO: 8