Patent ID: 7560276

Claim:
A method of stimulating tissue infiltration in a soft tissue defect in a patient in need thereof, said method comprising (a) contacting mammalian umbilical cord tissue that is substantially free of blood with a composition comprising dispase and at least one of hyaluronidase and collagenase, thereby yielding isolated cells; (b) optionally expanding said isolated cells in culture; (c) placing said isolated or expanded, isolated cells onto a scaffold, thereby yielding a scaffold comprising cells; and then (d) administering to said soft tissue defect of said patient said scaffold comprising cells; wherein said isolated or expanded, isolated cells are capable of self-renewal and differentiation; wherein said isolated or expanded, isolated cells do not express CD117; and wherein said isolated or expanded, isolated cells exhibit increased expression relative to a human cell that is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell of an endogenous gene encoding oxidized low density lipoprotein receptor 1, an endogenous gene encoding interleukin 8, and an endogenous gene encoding reticulon 1; and wherein said scaffold comprising cells stimulates tissue infiltration into said soft tissue defect.