Patent ID: 8435733

Claim:
A method of determining whether a donor tissue intended for transplant or transfusion is likely to be rejected in a human recipient wherein the human recipient expresses antibodies that specifically bind the amino acid sequence of SEQ ID NO: 1 or an antigenic fragment thereof, comprising a) obtaining a sample of the tissue intended for transplant or transfusion, b) extracting nucleic acids from the sample, c) contacting the nucleic acids with oligonucleotide probes that hybridize to a fragment of SEQ ID NO: 3, and d) detecting hybridization of the probe to the nucleic acids, wherein hybridization of the probe indicates the presence of an HNA-3a nucleic acid wherein HNA-3a nucleic acid comprises the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 1 or an antigenic fragment thereof, and wherein the presence of HNA-3a nucleic acid in the sample indicates that the sample is likely to be rejected in a human recipient that expresses HNA-3a specific antibodies.