Patent ID: 8148065

Claim:
A method for amplifying a nucleic acid comprising a target sequence comprising the steps of: (i) generating a single-stranded template comprising the target sequence; (ii) hybridizing a pair of oligonucleotides to the template such that the oligonucleotides are juxtaposed for ligation on the template and each oligonucleotide is positioned to flank the target sequence; (iii) ligating the juxtaposed oligonucleotides to produce a double-stranded ligation product; (iv) denaturing the double stranded ligation product to produce an initial template strand and an oligonucleotide ligation product strand; (v) repeating steps (ii)-(iv) such that both the initial template strand and the oligonucleotide ligation stand act as templates for a further ligation reaction.