Patent ID: 7888073

Claim:
A method for sequencing a target polynucleotide, comprising: (i) contacting the target polynucleotide with a polymerase enzyme and one or amore nucleotides selected from the group consisting of A, T (U), G, and C under conditions suitable for a polymerase reaction to proceed, wherein the contact occurs in a flow cell comprising one or more immobilized polynucleotides; (ii) observing the interaction of the polymerase enzyme with the target polynucleotide in the presence of the one or more nucleotides; and (iii) measuring the time taken for the polymerase to bind to, and subsequently dissociate from, the target polynucleotide to identify whether the polymerase has incorporated a nucleotide onto a nascent polynucleotide paired with the target polynucleotide, and thereby identify the sequence of the target polynucleotide.