Patent ID: 8476041

Claim:
A method for increasing the production of a desired product in a PTS − /Glu − bacterial host cell originally capable of utilizing a PTS for carbohydrate transport comprising, a) transforming a bacterial host cell having a PTS − /Glu − phenotype with a DNA construct comprising a promoter, wherein said DNA construct is chromosomally integrated into the PTS − /Glu − host cell replacing an endogenous promoter which is operably linked to a nucleic acid encoding a glucose assimilation protein; b) culturing the transformed bacterial host cell under suitable conditions; c) allowing expression of the glucose assimilation protein to obtain a host cell having a PTS − /Glu + phenotype; and d) obtaining an increased amount of a desired product in the transformed bacterial host cell compared to the amount of the desired product produced in a corresponding PTS bacterial cell cultured under essentially the same culture conditions, wherein said desired product is selected from the group consisting of pyruvate, PEP, lactate, acetate, glycerol, ethanol, succinate and chorismate.