Patent ID: 8252591

Claim:
A cell culture medium that supports proliferation of tumorigenic, transformed primary breast epithelial progenitor cells, comprising: (a) vitamin A, vitamin C, and vitamin D; (b) Zn, Mg, and Cu; (c) transferrin; (d) selenium; (e) cholesterol, linoleic acid, and lipoic acid; (0 hydrocortisone and estrogen; (g) triiodothyronine; (h) a carrier protein; (i) glutathione; (j) adenosine triphosphate; (k) phosphoethanolamine; (l) a nucleotide salvage pathway precursor base selected from the group consisting of hypoxanthine, xanthine, adenine, guanine and thymidine; (m) insulin; and (n) epidermal growth factor, wherein the medium is free of animal serum and tissue extracts and supports proliferation of tumorigenic, transformed primary breast epithelial progenitor cells for at least about 35 population doublings (PD) in vitro.