Patent ID: 8030037

Claim:
A method of preparing a concentrated microalgae composition comprising: cultivating microalgae photoautotrophically outdoors in open ponds under filtered sunlight in continuous or batch mode at a dilution rate of less than 35% per day; harvesting the microalgae in exponential phase when cell number is increasing at a rate of at least 20% of maximal rate of growth; and concentrating the microalgae to obtain a concentrated microalgae composition having at least 40% of lipids in the microalgae in the form of glycodiacylglycerides, phosphodiacylglycerides, or a combination thereof and at least 5% by weight of fatty acids are docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof; wherein (a) the microalgae are Tetraselmis sp. and EPA yield in the microalgae is at least 10 mg/liter culture, (b) the microalgae are Isochrvsis sp. or Pavlova sp., (c) the microalgae are Thalassiosira sp. or Chaetecoros sp. and EPA yield in the microalgae is at least 10 mg/liter culture, or (d) the microalgae are Tetraselmis sp. and are cultivated at above 20° C. and EPA yield in the microalgae is at least 10 mg/liter culture.