Patent ID: 8425845

Claim:
A microfluidic device comprising: a supporting substrate; an inlet for receiving a biological sample containing target nucleic acid sequences; hybridization chambers containing probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid; a calibration chamber with no fluorophore; and, a photosensor positioned adjacent the hybridization chambers and the calibration chamber for sensing which of the probes generate the fluorescence signal in response to the excitation light; wherein, the hybridization chambers and the calibration chamber have an optical window to expose the probes to the excitation light, the photosensor being an array of photodiodes positioned on the supporting substrate in registration with the hybridization chambers and the calibration chamber.