Patent ID: 7135289

Claim:
A chromatographic method for separating heteroduplex and homoduplex DNA molecules in a test mixture, said method comprising: (a) applying the test mixture to a reverse phase separation medium; (b) eluting the medium of step (a) with a mobile phase comprising betaine, wherein said eluting is carried out at 20° C. to 80° C. and wherein the eluting results in the separation of said heteroduplexes from said homoduplexes; wherein said eluting is carried out at a pre-selected concentration of betaine and at a pre-selected temperature; wherein said betaine is present at a concentration at which first heteroduplex molecules from a first mixture can be observed at said pre-selected temperature, and wherein second heteroduplex molecules from a second mixture can be observed at said pre-selected temperature; wherein said first mixture comprises a hybridization product of the double stranded sequence variant 168A and sequence variant 168G, wherein the 168A variant corresponds to the nucleic acid identified by SEQ ID NO:1; and, wherein said second mixture comprises a hybridization product of the double stranded sequence variant 46C and sequence variant 46G, wherein the 46C variant corresponds to the nucleic acid identified by SEQ ID NO:2.