Patent ID: 8536316

Claim:
A method of purifying an Fc region containing target protein from one or more impurities in a sample, the method comprising the steps of (a) contacting the sample with an affinity chromatography media; (b) eluting the Fc region containing target protein from the affinity media; (c) contacting the eluate with a cation exchange chromatography media; (d) contacting the cation exchange chromatography media with a buffer having a pH lower than 4.0 for a period time suitable for viral inactivation, wherein the buffer conductivity is 5 to 50 mS; (e) eluting the Fc region containing target protein from the cation exchange chromatography media; (f) contacting the eluate with an anion exchange chromatography media; and (g) recovering the Fc region containing target protein, wherein the method eliminates the need for a holding tank between steps (b) and (c) and steps (e) and (f).