Patent ID: 7364872

Claim:
A method of detecting a constituent in a test sample, comprising: providing a test element comprising, a support defining a test region, the test region having an electronically discernible property that can be altered when an enzyme induced metal deposit is present in the test region a capture target provided in the test region, and a sensing element electrically connectable with the test region; contacting the test region to the test sample; contacting the test region to an enzyme so that the enzyme will associate with the constituent if the constituent is present in the test region; contacting the test region to a metal ion; contacting the test region to a reducing agent and an oxidizing agent; wherein after the steps of contacting if the constituent is present in the test region the enzyme associated with the constituent in conjunction with the reducing agent and the oxidizing agent will induce reduction of the metal ion to elemental metal; deposition of elemental metal in the test region so that the test region property will be altered; detecting the alteration of the test region property; and correlating the alteration of the test region property to detection of the constituent in the test sample.