Patent ID: 6879440

Claim:
An autofocus module for a microscope-based system, comprising: a first light source configured to generate a first light beam for focusing; a second light source configured for generating a second light beam for focusing; an optical directing device configured to direct a respective portion of each of the first and second light beams onto an incoupling device, the incoupling device being configured to couple the respective portion of each of the first and second light beams into an illuminating light beam of the microscope-based system and direct the respective portion of each of the first and second light beams onto a specimen, the illuminating light beam being perpendicular to a surface of the specimen and propagating along an optical axis defined by an objective of the microscope-based system; a first detector configured to receive a third light beam of only the first light source, the third light beam being reflected from the specimen; and a second detector configured to receive a fourth light beam of only the second light source, the fourth light beam being reflected from the specimen.