Patent ID: 7786294

Claim:
A method of making a polydeoxynucleotide comprising the steps of (a) obtaining a ribonucleotide from a sample and placing it in a buffer containing deoxynucleotide triphosphates, (b) adding to the ribonucleotide a mixture of at least two reverse transcriptases comprising at least one reverse transcriptase with reduced RNase H activity, (c) adding to the ribonucleotide and mixture of at least two reverse transcriptases, a reverse transcription oligonucleotide primer, as set forth in SEQ ID NO: 25, to obtain a reverse transcription cocktail, (d) heating the reverse transcription cocktail to 50° C. for at least about 75 minutes followed by heating to 70° C., to produce a complementary polydeoxynucleotide (“cDNA”), followed by, (e) addition of (i) a first deoxynucleotide polymerase oligonucleotide primer, (ii) a second deoxynucleotide polymerase oligonucleotide primer, (iii) a Trnc-21 molecule, and (iv) a deoxynucleotide polymerase to the reverse transcription cocktail plus cDNA mixture, to obtain a polymerase chain reaction (“PCR”) cocktail, (f) heating the PCR cocktail to about 94° C. for about one (1) minute, (g) followed by 10 cycles of 94° C. for 30 seconds, followed by 72° C. for 5-10 minutes, and then (h) 20 cycles in which the annealing/elongation temperature is reduced to 60° C.