Patent ID: 7806980

Claim:
A method of crystallizing a complex of a purified human beta secretase polypeptide and a ligand, wherein the purified human beta secretase polypeptide comprises the amino acid sequence of SEQ ID NO:1 and the ligand has the formula N1-((2S,3R)-1-(3,5-difluorophenyl)-3-hydroxy-4-(3-iodobenzylamino)butan-2yl)-5-methyl-N3,N3-dipropylisophthalamide, the method comprising combining equal volumes of (a) an aqueous solution of 40 mg/mL of the purified human beta secretase polypeptide and 2mM of the ligand, and (b) a solution of 17% PEG 3000, 0.1 M sodium acetate pH 4.5 at 20 degrees C. and crystallizing a complex of the purified human beta secretase polypeptide and the ligand.