Patent ID: 7553626

Claim:
A method of detecting the presence of one or more bacteria selected from the group consisting of Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus , and Streptococcus pneumoniae , the method comprising: carrying out PCR using nucleic acids isolated from a sample containing bacteria as a template and a primer set as a primer, the primer set for amplifying a target sequence specific to Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus , and Streptococcus pneumoniae , the primer set comprising an oligonucleotide consisting of SEQ ID NO: 1 and an oligonucleotide consisting of SEQ ID NO: 2; and detecting the obtained PCR product using a probe oligonucleotide set as probes, wherein the probe oligonucleotide set is specific for target nucleic acids of bacteria consisting of Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus , and Streptococcus pneumoniae , the probe oligonucleotide set comprising: oligonucleotides of SEQ ID NOS: 3 and 4, which are specifically hybridizable with a nucleic acid from Bordetella pertussis and which do not cross-hybridize with a nucleic acid from Chlamydophila pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus , and Streptococcus pneumoniae.