Patent ID: 7371569

Claim:
An expression vector replicable in mammalian cells, comprising the following elements grouped in a way that assures a correct and efficient transcription of a fragment comprising the complete sequence encoding the human thrombopoictin (hTPO) polypeptide including its signal peptide (eTPO): (i) a hTPO transcription portion consisting of: a) a transcription promoter of a variable kappa gene of human immunoglobulins b) a DNA segment encoding the complete hTPO polypeptide including its signal peptide c) an initnunoglobulin transcription enhancer DNA segment of the human kappa gene in a 3′ location with respect to hTPO gene, wherein said enhancer DNA segment consists of the DNA sequence 5′ to the Ck region of the human kappa gene d) a transcription terminator e) a polyadenylation signal; and (ii) gene encoding resistance to the antibiotic neomicyn type drugs (iii) a gene encoding resistance to the antibiotic ampicillin; and (iv) a replication origin for E Coli (ColEI ori).