Patent ID: 7763427

Claim:
A process of detecting the presence or absence of a recombinase polymerase amplification amplified target nucleic acid, comprising the steps of: (a) contacting a recombinase agent with a first and second nucleic acid primer to form a first and second nucleoprotein primer, wherein each of said nucleic acid primers comprise a single stranded region at its 3′ end, wherein said first nucleic acid primer is labeled with a first member of a first binding pair, and wherein said second nucleic acid primer is labeled with a first member of a second binding pair; (b) contacting the first and the second nucleoprotein primers to a sample suspected to contain an amplified target nucleic acid amplified by a recombinase polymerase amplification (RPA) process to form complexes with a first and a second nucleoprotein primer/target nucleic acid structure at a first portion and a second portion of said amplified target nucleic acid, respectively, and wherein said recombinase polymerase amplification process is performed in the presence of a crowding agent such that the crowding agent stimulates amplification; (c) contacting said complexes with a first mobile solid support coated with a second member of said first binding pair and a second mobile solid support coated with a second member of said second binding pair; (d) determining if said first mobile solid support is co-localized with said second mobile solid support to determine the presence of said recombinase polymerase amplification amplified target nucleic acid.