Patent ID: 8071536

Claim:
A DNA polymerase, comprising R-X 1 -X 2 -X 3 -K-L-X 4 -X 5 -X 6 -Y-X 7 -X 8 -X 9 -X 10 -X 11 , wherein X 1 is selected from the group consisting of E, Q, G, K, and T, X 2 is L, I, Y, X 3 is selected from the group consisting of T, M, D, S, G, A, Q, and L, X 4 is K, R, Q, X 5 is N, S, G, X 6 is S, X 7 is selected from the group consisting of V, I, L, A, T, X 8 is D or E, X 9 is selected from the group consisting of P, A, G, K, T, and S, X 10 is L or I, X 11 is P or L (SEQ ID NO:60); wherein the polymerase has improved pyrophosphorolysis activated polymerization (PAP) relative to an otherwise identical polymerase where X 6 is T.