Patent ID: 8129146

Claim:
A process for making a human insulin precursor or a human insulin precursor analog, said process comprising (i) culturing a host cell comprising a polynucloetide sequence encoding an insulin precursor or an insulin precursor analog under suitable culture conditions for expression of said precursor or precursor analog; and (ii) isolating the expressed precursor or precursor analog, wherein said human insulin precursor or human insulin analog precursor comprises a sequence of the formula: B(1-27)-X 3 —X 2 —X 1 —Y-A(1-21), wherein X 1 is 1-5 amino acid residues in length and comprises at least one Gly and a Gly is immediately N-terminal to Y, X 2 is one of Pro, Lys, Ala, Arg or Pro-Thr at position 29 of the B chain, X 3 is one of Pro, Asp, Lys, or Ile at position 28 of the B chain, and Y is Lys or Arg, and wherein X 1 —Y does not contain two adjacent basic amino acids.