Patent ID: 7816133

Claim:
A method for in vitro growing of connective tissue substitute, said connective tissue substitute being populated with fibroblasts, said connective tissue substitute being suitable for application onto a wound in a subject in need thereof, said method comprising the steps of: a) providing a connective tissue layer substantially free of living cells having a first and a second contacting side, the first contacting side being opposed to the second contacting side; b) placing the connective tissue layer in a container comprising fibroblasts, allowing the fibroblasts to contact the first contacting side of the connective tissue layer; and c) at least temporarily simultaneous with step b) contacting the second contacting side of the connective tissue layer, the connective tissue layer being positioned in the container, with an intact epithelial layer, to attract said fibroblasts into the connective tissue layer by passing through the first contacting side of the said connective tissue layer.