Patent ID: 8574907

Claim:
A mammalian cell culture medium for the production of antibodies or antibody derivatives having reduced core fucosylation, comprising an effective amount of a fucose analog, wherein the fucose analog is selected from the group consisting of one of the following formulae (III) or (IV): or a biologically acceptable salt or solvate thereof, wherein each of formula (III) or (IV) can be the alpha or beta anomer or the corresponding aldose form; each of R 1 -R 4 is independently selected from the group consisting of fluoro, chloro, —OH, —OC(O)H, —OC(O)C 1 -C 10 alkyl, —OC(O)C 2 -C 10 alkenyl, —OC(O)C 2 -C 10 alkynyl, —OC(O)aryl, —OC(O)heterocycle, —OC(O)C 1 -C 10 alkylene(aryl), —OC(O)C 2 -C 10 alkenylene(aryl), —OC(O)C 2 -C 10 alkynyl(aryl), —OC(O)C 1 -C 10 alkylene heterocycle, —OC(O)C 2 -C 10 alkenylene(heterocycle), —OC(O)C 2 -C 10 alkynyl heterocycle, —OCH 2 OC(O) alkyl, —OCH 2 OC(O)O alkyl, —OCH 2 OC(O) aryl, —OCH 2 OC(O)O aryl, —OC(O)CH 2 O(CH 2 CH 2 O) n CH 3 , —OC(O)CH 2 CH 2 O(CH 2 CH 2 O) n CH 3 , —O-tri-C 1 -C 3 alkylsilyl and —OC 1 -C 10 alkyl, wherein each n is an integer independently selected from 0-5; and each of R 2a and R 3a is independently selected from the group consisting of H, F and Cl; R 5 is selected from the group consisting of —CH 3 , —CHF 2 , —CH═C═CH 2 , —C≡CH, —C≡CCH 3 , —CH 2 C≡CH, —C(O)OCH 3 , —CH(OAc)CH 3 , —CN, —CH 2 CN, —CH 2 X (wherein X is Br, Cl or I), and methoxiran; wherein when R 5 is other than —CH═C═CH 2 or —CHF 2 , at least one of R 1 , R 2 , R 3 , R 2a and R 3a is fluoro or chloro.