Patent ID: 8715929

Claim:
A method of expressing a gene of interest in H. pylori , the method comprising: a) integrating an operon into chromosomal DNA of a H. pylori bacterium, wherein the operon comprises a promoter operably-linked to at least two genes, wherein at least one gene is a gene of interest and at least one gene is a H. pylori urease gene, wherein the gene of interest is positioned within the operon between a urease A (ureA) gene and a urease B (ureB) gene or after a ureB gene, such that transcription of the gene of interest is linked to transcription of subunits A and B of a urease complex, and wherein the promoter is arranged such that a polycistronic mRNA comprising both the gene of interest and the H. pylori urease gene is transcribed; and b) culturing said bacterium to express the gene of interest.