Patent ID: 8455245

Claim:
A method of obtaining an isolated substantially pure preparation of ABCB5 positive immunomodulatory dermal mesenchymal stem cells comprising: a) obtaining a sample of dermal tissue that has been separated from epidermal tissue from a human donor; b) isolating ABCB5 positive cells from the dermal tissue sample, wherein the ABCB5 positive cells are isolated using immobilized antibody against ABCB5 c) expanding the ABCB5 positive cells by limiting dilution cloning to produce the isolated substantially pure preparation of ABCB5 positive immunomodulatory dermal mesenchymal stem cells, and d) formulating the ABCB5 positive cells in a pharmaceutically acceptable carrier for administration to a human subject, wherein ABCB5 positive dermal cells comprise at least 95% of the isolated substantially pure preparation of ABCB5 positive immunomodulatory dermal mesenchymal stem cells.