Patent ID: 8454972

Claim:
A method of inducing an immune response against HIV in a human, which method comprises (1) administering a first composition to a human, wherein the first composition comprises (a) a plasmid comprising a nucleic acid sequence encoding an HIV Env protein from Glade A, (b) a plasmid comprising a nucleic acid sequence encoding an HIV Env protein from Glade B, (c) a plasmid comprising a nucleic acid sequence encoding an HIV Env protein from Glade C, (d) a plasmid comprising a nucleic acid sequence encoding an HIV Gag protein from Glade B, (e) a plasmid comprising a nucleic acid sequence encoding an HIV Pol protein from Glade B, (f) a plasmid comprising a nucleic acid sequence encoding an HIV Nef protein from Glade B, and (g) a pharmaceutically acceptable carrier, and (2) administering a second composition to the human after the administration of the first composition to the human, wherein the second composition comprises (a) an adenoviral vector comprising a nucleic acid sequence encoding an HIV Env protein from Glade A, (b) an adenoviral vector comprising a nucleic acid sequence encoding an HIV Env protein from Glade B, (c) an adenoviral vector comprising a nucleic acid sequence encoding an HIV Env protein from Glade C, (d) an adenoviral vector comprising a nucleic acid sequence encoding an HIV Gag-Pol fusion protein from Glade B, and (e) a pharmaceutically acceptable carrier, whereupon an immune response against HIV is induced in the human.