Patent ID: 7125843

Claim:
A method of forming a covalent conjugate between a first glycosylated or non-glycosylated peptide, and a second glycosylated or non-glycosylated peptide cojoined by a linker moiety, wherein said linker moiety is conjugated to said first peptide via a first intact glycosyl linking group interposed between and covalently linked to both said first peptide and said linker moiety, and said linker moiety is conjugated to said second peptide via a second intact glycosyl linking group interposed between and covalently linked to both said second peptide and said linker moiety; said method comprising: (a) contacting said first peptide with a precursor of said linker moiety comprising a precursor of said first intact glycosyl linking group and a precursor of said second intact glycosyl linking group; (b) contacting the mixture from (a) with a glycosyl transferase for which said precursor of said first glycosyl linking group is a substrate, under conditions sufficient to convert said precursor of said first intact glycosyl linking group into said first intact glycosyl linking group, thereby forming a first conjugate between said linker moiety precursor and said first peptide; (c) contacting said first conjugate with said second peptide and a glycosyltransferase for which said precursor of said second intact glycosyl group is a substrate under conditions sufficient to convert said precursor of said second intact glycosyl linking group into said second glycosyl linking group, thereby forming said conjugate between said linker moiety and said first glycosylated or non-glycosylated peptide, and said second glycosylated or non-glycosylated peptide.