Patent ID: 7358067

Claim:
A method of producing a heterologous peptide, polypeptide or protein in a Lactococcus lactis bacterium, the method comprising the steps of: (i) constructing a recombinant Lactococcus lactis bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein and operably linked thereto, appropriate regulatory nucleotide sequences to control the expression of the coding sequence, (ii) cultivating said recombinant bacterium under fed-batch or continuous cultivation conditions in a chemically defined medium, to express the nucleotide sequence, and (iii) harvesting the recombinant bacterium or the peptide, polypeptide or protein, wherein the concentration of glucose is kept at a pre-selected concentration of at least about 0.5 g/L by controlled feeding of glucose.