Patent ID: 8172996

Claim:
A method for producing an enzyme electrode used to detect glucose, comprising: providing a substrate having a carbon surface, wherein the substrate is a pencil lead, and the carbon surface is formed by coating a carbon paste on the surface of the pencil lead; forming a gold surface on the carbon surface and thus forming an electrode; modifying the gold surface by L-cysteine to bond covalently the gold particles of the gold surface and the sulphydryl group of L-cysteine, thereby forming a first electrode having a first modified surface; modifying the first modified surface by N,N′-dicyclohexylcarbodiimide, the L-cysteine of the first modified surface and the N,N′-dicyclohexylcarbodiimide being dehydrated to form covalent bonds, thereby forming a second electrode having a second modified surface; and contacting the second modified surface with the glucose oxidase, an amide bond being formed between the L-cysteine group of the second modified surface and the glucose oxidase, thereby forming a third electrode having a third modified surface.