Patent ID: 8071110

Claim:
A recombinant single-chain polypeptide comprising: a) a first amino acid sequence region comprising i) a first domain comprising a binding element comprising a peptide able to preferentially interact with a peptide receptor under physiological conditions; and ii) a second domain comprising a translocation element comprising a Clostridial toxin translocation domain able to facilitate the transfer of a Clostridial neurotoxin light chain across an intracellular vesicular membrane; and b) a second amino acid sequence region comprising a therapeutic element comprising a Clostridial neurotoxin light chain having biological activity when released into the cytoplasm of said target cell; c) a third amino acid sequence region comprising an exogenous protease cleavage site; wherein the peptide is a glucagon like hormone; and wherein said first and second amino acid sequence regions are separated by said third amino acid sequence region.