Patent ID: 8323894

Claim:
A method of assessing the genomic imprinting status of a cloned embryo, which comprises: extracting both a genomic DNA sample and an RNA sample from the cloned embryo; simultaneously hybridizing both the genomic DNA sample and the RNA sample to an array comprising a plurality of probes designed to interrogate a plurality of single nucleotide polymorphisms (SNPs), wherein the genomic DNA sample and the RNA sample are differentially labeled; analyzing data from the hybridizing step to generate an imprinted gene expression pattern, wherein generating the imprinted gene expression pattern comprises identifying at least one of the plurality of SNPs that is both heterozygous in the genomic DNA sample and homozygous in the RNA sample; comparing the imprinted gene expression pattern to a database of imprinted gene expression patterns from a plurality of normal embryos at an identical developmental stage; determining the parental origin of at least one gene in the cloned embryo by genotyping both parents and comparing at least one parental genotype to the imprinted gene expression pattern of the cloned embryo; and assessing if the genomic imprinting status of the cloned embryo is normal, wherein the genomic imprinting status is normal if the imprinted gene expression pattern from the cloned embryo matches the database.