Patent ID: 8586310

Claim:
A method of amplifying at least two different nucleic acid sequences, the method comprising: a) creating known 5′ and 3′ ends of at least two nucleic acid sequences by: i) annealing an upstream primer and a downstream primer to the at least two nucleic acid sequences to be amplified; ii) amplifying the at least two nucleic acid sequences so as to create amplicons; and iii) after amplifying, removing the upstream and downstream primer sequences from the amplicons of step ii); b) annealing an upstream and a downstream nucleic acid patch to each of the amplicons of step iii), and annealing an upstream universal primer to the upstream patch, and a downstream universal primer to the downstream patch; c) ligating the upstream universal primer and the downstream universal primer to the associated amplicon; and d) amplifying the ligated nucleic acid sequences of step (c).