Patent ID: 7521066

Claim:
A method of treating a targeted tissue or cell comprising administering to said targeted tissue or cell an effective amount of a pharmaceutical composition comprising non-gas containing nanoparticles capable of being internalized within a target tissue or cell sufficient to achieve a desired effect comprising, said non-gas containing nanoparticles comprising: a mixture of select lipids comprising: a) at least one first member selected from the group consisting of glycerol monoesters of saturated carboxylic acids containing from about 10 to 18 carbon atoms and aliphatic alcohols containing from about 10 to 18 carbon atoms; b) at least one second member selected from the group consisting of sterol aromatic acid esters; c) at least one third member selected from the group consisting of sterols and sterol esters of aliphatic carboxylic acids containing from about 1 to 18 carbon atoms; d) at least one fourth member selected from the group consisting of glycerol and glycerol di- and tri-esters of aliphatic carboxylic acids containing from about 10 to 18 carbon atoms and aliphatic alcohols containing from about 10 to 18 carbon atoms; and e) at least one therapeutically active agent, wherein said pharmaceutical composition is produced by processing through a microfluidizer.