Patent ID: 8207320

Claim:
An isolated polynucleotide comprising (H 2 ), (I 2 ), (J 2 ), (K 2 ), (L 2 ), (M 2 ) or (N 2 ) shown below: (H 2 ) an isolated polynucleotide which consists of all of the nucleotide sequence shown in SEQ ID NO: 28 or a part thereof covering at least nucleotides 414-1688 or 498-1685; (I 2 ) an isolated polynucleotide which is hybridizable under stringent conditions with a polynucleotide consisting of a nucleotide sequence complementary to the nucleotide sequence of the polynucleotide shown in (H 2 ) and which encodes a protein having lipase activity; (J 2 ) an isolated polynucleotide which consists of a nucleotide sequence comprising substitution, deletion, insertion and/or addition of 1 to 9 nucleotides in the nucleotide sequence of the polynucleotide shown in (H 2 ) and which encodes a protein having lipase activity; (K 2 ) an isolated polynucleotide which shares an identity of at least 80% or more with the nucleotide sequence of the polynucleotide shown in (H 2 ) and which encodes a protein having lipase activity; (L 2 ) an isolated polynucleotide which encodes a protein consisting of all of the amino acid sequence shown in SEQ ID NO: 29 or a part thereof covering at least amino acids 29-424; (M 2 ) an isolated polynucleotide which encodes a protein consisting of an amino acid sequence comprising substitution, deletion, insertion and/or addition of 1 to 9 amino acids in the amino acid sequence of the protein shown in (L 2 ) and having lipase activity; or (N 2 ) an isolated polynucleotide which encodes a protein consisting of an amino acid sequence sharing an identity of at least 80% or more with the amino acid sequence of the protein shown in (L 2 ) and having lipase activity, wherein the stringent condition consists of hybridization at 65° C. in a solution containing 6 M urea, 0.4% sodium dodecyl sulfate (SDS), and 0.1×saline sodium citrate (SSC).