Patent ID: 7338800

Claim:
A method of DNA transfer in vivo, comprising the steps of: conjugating a first bacteria having a donor vector comprising a polynucleotide of interest with a second bacteria having a recipient vector, wherein the conjugation results in transfer of the donor vector to the second bacteria; and inserting the polynucleotide of interest from the donor vector into the recipient vector by homologous recombination; wherein the donor vector is further defined as comprising: a polynucleotide of interest; a first homology region; and a second homology region, and wherein the recipient vector is further defined as comprising: a first homology region; and a second homology region; wherein the homologous recombination is further defined as homologously recombining said polynucleotide of interest into said vector at said homology regions, wherein a double strand break is generated in the recipient vector by restriction enzyme digestion, wherein said restriction enzyme is inducibly expressed in said second bacteria.