Patent ID: 7144487

Claim:
A method for isolating Factor VIII (FVIII) from a sample containing FVIII and unwanted molecules comprising: (a) introducing the sample into a first interstitial volume of a solvent at a pH between about 6.5 and about 7.0 in an electrophoresis apparatus comprising a separation membrane having a defined pore size with a molecular mass cut off different from the molecular mass of FVIII, a first restriction membrane disposed between a first electrode zone and the separation membrane so as to define the first interstitial volume therebetween, and a second restriction membrane disposed between a second electrode zone and the separation membrane so as to define a second interstitial volume therebetween; (b) adding one or more stabilizing agents to one or more of the sample, the first interstitial volume or the second interstitial volume, wherein the one or more stabilizing agents is present at a concentration of between about 2 g/L and about 50 g/L; (c) applying an electric potential between the first and second interstitial volumes such that the FVIII is located on one side of the separation membrane and the unwanted molecules are substantially located on the other side of the separation membrane; and (d) maintaining step (c) until a desired amount of the FVIII is isolated.