Patent ID: 6964861

Claim:
A method for cloning or subcloning one or more desired nucleic acid molecules comprising (a) forming a mixture by combining in vitro (i) one or more first nucleic acid molecules comprising one or more desired nucleic acid segments flanked by at least two recombination sites, wherein said recombination sites do not recombine with each other; (ii) one or more second nucleic acid molecules each comprising at least two recombination sites, wherein said recombination sites do not recombine with each other; (iii) at least one recombination protein; and (iv) at least one ribosomal protein that is present in an amount sufficient to enhance recombinational cloning; and (b) incubating said mixture under conditions sufficient to transfer one or more of said desired segments into one or more of said second nucleic acid molecules, thereby producing one or more desired third nucleic acid molecules.