Patent ID: 8630833

Claim:
A method of diagnosing cancer in a test set of biological cells in an n-dimensional space, comprising: exposing each cell in a normal set of biological cells to a plurality of four or more reagents using a first protocol; measuring a corresponding plurality of fluorescence intensities of each cell in the normal set of biological cells using a second protocol; mapping each cell in the normal set of biological cells to a corresponding point in an n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the normal set of biological cells, wherein the corresponding points form a normal set of points; defining, using one or more configured computing systems, a set of normal clusters in the n-dimensional space by defining a centroid line and radius based on the mapping of the normal set of points in the n-dimensional space, wherein, each cluster in the set of normal clusters corresponds to a maturation level within a cell lineage; the centroid line is defined based on a set of reference points; and the radius is a distance from a nearest point on the defined centroid line; exposing each cell in a test set of biological cells to the plurality of reagents using the first protocol; measuring a corresponding plurality of fluorescence intensities of each cell in the test set of biological cells using the second protocol; mapping each cell in the test set of biological cells to a corresponding point in an n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the test set of biological cells, wherein the corresponding points form a test set of points; comparing the test set of points to the defined set of normal clusters; and diagnosing cancer in the test set of biological cells based on the comparison of the test set of points to the defined set of normal clusters.