Patent ID: 7195896

Claim:
A system comprising: (i) an adenoviral vector comprising an adenoviral genome having a deficiency in one or more essential gene functions of the E1 region of the adenoviral genome and a deficiency in one or more essential gene functions in either or both of the E2A region and the E4 region of the adenoviral genome, and optionally a deficiency in the E3 region of the adenoviral genome, and (ii) a cell having a cellular genome that complements in trans for the deficiency in one or more essential gene functions of the E1 region of the adenoviral genome and the deficiency in one or more essential gene functions in either or both of the E2A region and the E4 region, of the adenoviral genome, wherein (a) if the adenoviral vector is deficient in one or more essential gene functions of the E2A region, the cellular genome comprises a nucleic acid sequence that complements for the deficiency in the E2a region that is operably linked to an inducible promoter or a repressible promoter, and (b) if the adenoviral vector is deficient in one or more essential gene functions of the E4 region, the cellular genome comprises a nucleic acid sequence that complements for the deficiency in the E4 region that is operably linked to an inducible promoter or a repressible promoter, and wherein there is no overlap between the cellular genome and the adenoviral genome that mediates a recombination event between the cellular genome and the adenoviral genome.