Patent ID: 8133484

Claim:
A method of treating a wounded tissue, comprising applying to said tissue a composition comprising gelatin and a non-toxic cross-linking agent, wherein said non-toxic cross-linking agent comprises transglutaminase, wherein said transglutaminase is included as part of a transglutaminase composition and the weight ratio of gelatin to transglutaminase composition is in a range of from about 1:10 to about 100:1, wherein activity of said transglutaminase in the gelatin-transglutaminase composition is at least about 40 U/g of gelatin, and wherein said gelatin and said transglutaminase are mixed before being applied to said tissue in liquid form; inducing hemostasis in said wound; and forming a biomimetic clot through in-situ cross-linking of said gelatin to said tissue by said transglutaminase and through formation of an in-situ matrix by said cross-linking.