Patent ID: 8685416

Claim:
A method of inducing an immune response to a tumor in a subject, comprising, selecting a subject with a tumor; and administering a therapeutically effective amount of apoptotic tumor cells conjugated to a CpG oligodexoynucleotide to the subject, wherein the CpG oligodeoxynucleotide is a K-type CpG oligodeoxynucleotide or a D-type CpG oligodeoxynucleotide wherein the K-type CpG oligodeoxynucleotide has a nucleic acid sequence set forth as: (SEQâ€ƒIDâ€ƒNO:â€ƒ2) 5â€² N 1 N 2 N 3 D-CpG-WN 4 N 5 N 6 3â€² wherein the central CpG motif is unmethylated, D is T, G or A, W is A or T, and N 1 , N 2 , N 3 , N 4 , N 5 , and N 6 are any nucleotide, wherein the CpG oligodeoxynucleotide is 10 to 30 nucleotides in length; and wherein the D-type CpG oligodeoxynucleotide has a sequence (SEQâ€ƒIDâ€ƒNO:â€ƒ1) 5â€²-X 1 X 2 X 3 Pu 1 Py 2 CpGâ€ƒPu 3 Py 4 X 4 X 5 X 6 (W) M (G) N -3â€² wherein the central CpG motif is unmethylated, Pu is a purine nucleotide, Py is a pyrimidine nucleotide, X and W are any nucleotide, M is any integer from 0 to 10, and N is any integer from 4 to 10, wherein the CpG ODN is 18 to 50 nucleotides in length; thereby inducing the immune response to the tumor in the subject.