Patent ID: 8883449

Claim:
A polynucleotide encoding a single-chain insulin polypeptide having the formula: B(1-26) -X 1 -X 2 -X 3 -X 4 -A(1-21) wherein X 1 is Thr, Lys, or Arg; X 2 is Pro, Lys, or Asp; X 3 is Lys, Pro, or Glu; X 4 is a peptide sequence selected from the group consisting of AGRGSGK (SEQ ID NO: 15); AGLGSGK (SEQ ID NO: 33); AGMGSGK (SEQ ID NO: 45); ASWGSGK (SEQ ID NO: 48); TGLGSGQ (SEQ ID NO: 22); TGLGRGK (SEQ ID NO: 23); TGLGSGK (SEQ ID NO: 21); HGLYSGK (SEQ ID NO: 50); KGLGSGQ (SEQ ID NO: 51); VGLMSGK (SEQ ID NO: 56); VGLSSGQ (SEQ ID NO: 27); VGLYSGK (SEQ ID NO: 28), VGLSSGK (SEQ ID NO: 30); VGMSSGK (SEQ ID NO: 65); VWSSSGK (SEQ ID NO: 76), VGSSSGK (SEQ ID NO: 16) and VGMSSGK (SEQ ID NO: 106), wherein B(1-26) is a peptide chain consisting of the first 26 amino acid residues of the B chain of human insulin counted from the N-terminal end of the B chain or an analogue or derivative thereof, and A(1-21) is the natural insulin A chain or an analogue thereof or derivative thereof.