Patent ID: 6872871

Claim:
A method of expressing PCA (protein fragment complementation assays) interacting protein partners in plant material comprising: (A) transforming said plant material with: (1) a first PCA interacting protein partner construct coding for a first fusion product comprising: (a) a first fragment of a dihydrofolate reductase enzyme which fragment associates with a second fragment of said dihydrofolate reductase enzyme to produce a detectable enzymatic activity and (b) a first protein-protein interacting domain; and (2) a second PCA interacting protein partner construct coding for a second fusion product comprising: (a) a second fragment of said dihydrofolate reductase enzyme and (b) a second protein-protein interacting domain that can bind said first protein-protein interacting domain; (B) culturing said material under conditions allowing expression of said PCA interacting protein partners, and allowing interaction of said first protein-protein interacting domain with said second protein-protein interacting domain; (C) directly or indirectly testing for reconstitution of said enzymatic activity when said dihydrofolate reductase enzyme fragments are associated.