Patent ID: 8129508

Claim:
A method for purifying a target protein from a mixture, which comprises the target protein and one or more contaminants, wherein the contaminants comprise host cell proteins, said method comprising purification steps consisting of: (a) subjecting the mixture to a cation exchange chromatography step and a hydrophobic charge induction chromatography step, in either order, wherein the hydrophobic charge induction chromatography step or the cation exchange chromatography step uses an elution buffer that elutes the host cell proteins differentially in relation to the target protein, and wherein the tailing of the Abs 280 peak of the target protein is not collected at less than 20% above the baseline as measured by Abs 280 relative to the maximum of the peak height; and (b) isolating the target protein to a purity of about 100 parts per million (ppm) or less of host cell proteins.