Patent ID: 6994868

Claim:
A method of inhibiting protein kinase activity in a biological sample, wherein said protein kinase is ERK, comprising the step of contacting said biological sample with a compound comprising Grp 1, Grp 2, and Grp 3, wherein: Grp 1 is an optionally substituted aryl or aliphatic group; Grp 2 is selected from wherein Grp 2 has a hydrogen bond acceptor HBA2, and wherein HBA2 is optionally bonded to a hydrogen bond donor HBD2; and Grp 3 is a 5-membered heteroaromatic ring having 1–4 nitrogens comprising a hydrogen bond donor HBD1; wherein said compound optionally comprises a hydrogen bond acceptor HBA1; and wherein Grp 1 is within about 2.5–10.0 Å of Grp 2; Grp 2 is within about 4.0–8.0 Å of Grp 3; Grp 3 is within about 5.0–12.0 Å of Grp 1; HBA2 is within about 6.5–11.0 Å of Grp 1; HBD1 is within about 6.5–8.5 Å of Grp 2; HBD1 is within about 3.5–5.5 Å of HBA1; and HBA1 is within about 6.7–14.0 Å of HBD2.