Patent ID: 8367381

Claim:
A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof, the method comprising: A) placing L-isoleucine in an aqueous solvent comprising a bacterial composition comprising a bacterium comprising an L-isoleucine dioxygenase selected from the group consisting of: (a) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID No: 2, 8, 13, 17, and 21, (b) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID No: 2, 8, 13, 17, and 21, but which includes one to 13 substitutions, deletions, insertions, additions, or inversions of one to 13 amino acids, and wherein said protein has L-isoleucine dioxygenase activity, and (c) a protein that is at least 98% homologous to an amino acid sequence selected from the group consisting of SEQ ID No: 2, 8, 13, 17, and 21, and which has L-isoleucine dioxygenase activity; and B) isolating (2S,3R,4S)-4-hydroxy-L-isoleucine from the aqueous solvent.