Patent ID: 7299134

Claim:
A method of correlating gene expression with protein expression in two or more biological samples, the method comprising the steps of: a) obtaining two or more biological samples; b) generating a gene expression profile of each sample; c) determining the nucleotide sequence of at least one mRNA in each gene expression profile; d) predicting the amino acid sequence of the polypeptide encoded by the at least one mRNA in each gene expression profile; e) predicting the mass of the polypeptide encoded by the at least one mRNA in each gene expression profile; f) generating a protein profile of polypeptides in each sample by mass spectrometry; and g) determining the presence in each protein profile of a polypeptide having a mass that is the same as the predicted mass of the encoded polypeptide, thereby identifying at least one protein that is expressed from a corresponding mRNA in each biological sample, thereby correlating gene expression with protein expression in two or more biological samples.