Patent ID: 7115374

Claim:
A kit for amplifying a target nucleic acid sequence that may be present in a biological sample, comprising: a first primer that consists of a 3′ terminal target-complementary sequence and optionally a first primer upstream sequence that is not complementary to said target nucleic acid sequence that is to be amplified, said 3′ terminal target-complementary sequence of said first primer consisting of 22–24 contiguous bases contained within SEQ ID NO:74, and said 3′ terminal target-complementary sequence of said first primer being fully contained within the sequence of SEQ ID NO:74, allowing for the presence of RNA and DNA equivalents; and a second primer that consists of a 3′ terminal target-complementary sequence and optionally a second primer upstream sequence that is not complementary to said target nucleic acid sequence that is to be amplifed, said 3′ terminal target-complementary sequence of said second primer consisting of 18–31 contiguous bases contained within SEQ ID NO:59, and said 3′ terminal target-complementary sequence of said second primer being fully contained within the sequence of SEQ ID NO:59, allowing for the presence of RNA and DNA equivalents.