Patent ID: 8715937

Claim:
A method of sample analysis comprising: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of said genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample; wherein: i. said amplifying is done using a first primer and a second primer; and ii. said first primer comprises a 3′ terminal nucleotide that base pairs with said point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in said locus with the exception of a single base mismatch within 6 bases of said 3′ terminal nucleotide; b) detecting the presence of said product in said amplified sample using a flap assay that employs: i. said first primer; and ii. a flap oligonucleotide that comprises a nucleotide that base pairs with said point mutation.