Patent ID: 8691581

Claim:
A method of determining a DNA tag which is a base sequence to be introduced into a genomic DNA sequence of an organism and an introduction site of the DNA tag into the genomic DNA sequence, the method comprising (A) a processing unit of a computer performing the following steps S 1 to S 8 : a step S 1 of obtaining a protein coding sequence from the genomic DNA sequence; a step S 2 of determining a region within the protein coding sequence obtained in step S 1 that has no influence on a biological function of the organism even if the sequence is subjected to a silent mutation, as a first coding sequence; a step S 3 of obtaining a plurality of second coding sequences each including a partial sequence in the first coding sequence and having a predetermined length and recording sites of the second coding sequences in the protein coding sequence; a step S 4 of obtaining one or more third coding sequences produced by subjecting the corresponding second coding sequences obtained in the step S 3 to a silent mutation; a step S 5 of judging whether or not each of the third coding sequences obtained in step S 4 satisfy a predetermined condition and determining one or more sequences among the third coding sequences judged to satisfy the predetermined condition as fourth coding sequences; a step S 6 of performing a homology search for each of the fourth coding sequences; a step S 7 of determining an NMS for each of the fourth coding sequences using the results of the homology search performed in step S 6 ; and a step S 8 of determining the fourth coding sequence corresponding to the NMS with the minimum value among the NMSs determined for each of the fourth coding sequences in step S 7 as the DNA tag, and determining the site of the second coding sequence corresponding to the fourth coding sequence determined as the DNA tag, as the introduction site, wherein: the predetermined condition comprises a condition where a polynucleotide including the third coding sequence or a complementary base sequence thereof is suitable as a primer; and the NMS indicates a degree of homology; and (B) recording the determined DNA tag and the introduction site to a recording unit.