Patent ID: 8906660

Claim:
A composition comprising Φ29-type (phi29-type) recombinant DNA polymerase, which recombinant polymerase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO: 1, and which recombinant polymerase comprises one or more mutation selected from the group consisting of an amino acid substitution at position K131, an amino acid substitution at position K132, a K135Q substitution, a K135S substitution, an H149D substitution, a Q183F substitution, a G197D substitution, a G197E substitution, an 1201E substitution, a K206E substitution, an A437N substitution, and a D510S substitution, wherein identification of positions is relative to SEQ ID NO: 1, and wherein said polymerase exhibits polymerase activity.