Patent ID: 8465920

Claim:
A method for detecting or amplifying a target nucleic acid in a sample of genomic DNA or of total RNA, comprising at least an oligonucleotide-oligocation conjugate A i -B j and comprising the steps of: extending an oligonucleotide-oligocation conjugate A i -B j of structure I to III with said target nucleic acid in a sample of genomic DNA as a template; detecting said target nucleic acid in said sample of genomic DNA with an oligonucleotide-oligocation conjugate A i -B j of structure I to X; or reverse transcribing said target nucleic acid of a sample of total RNA with an oligonucleotide-oligocation conjugate A i -B j of structure I to III; wherein in said conjugate A i -B j A i is an i-mer oligonucleotide with i=3 to 50, where A i is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, B j , moiety is attached to A i moiety or to linker to A i via a phosphodiester link, B j , being a j-mer organic oligocation moiety, with j=1 to 50, where B is: —HPO 3 —R 1 —(NH—R 2 ) n —NH—R 3 —O—, where R 1 , R 2 and R 3 identical or different, are a C1-C6 alkylene radical, the NH—R 2 moieties being identical or different when n is >1; —HPO 3 —R 1 —CH(X)—R 3 —O— where R 1 and R 3 , identical or different, are a C1-C6 alkylene radical and X is putrescine, spermidine or a spermine residue, said structures Ito X being as follows: HO— 3′ A i 5′ -B j -R 4 structure I HO— 3′ A i 5′ -R 5 —B j -R 4 structure II HO— 3′ A i1 5′ —B j -A i2 -R 4 structure III R 4 —B j - 3′ A i 5′ —R 6 structure IV R 4 —B j -R 5 3′ A i 5′ -R 6 structure V R 7 - 3′ A i 5′ -B j —R 4 structure VI R 7 - 3′ A i 5′ -R 5 —B j —R 4 structure VII R 7 - 3′ A i1 5′ -B j - 3′ A i2 5′ -R 4 structure VIII R 7 - 3′ A i1 5′ -B j - 5′ A i2 3′ -R 8 structure IX R 4 —B j1 - 3′ A i 5′ -B j2 -R 6 structure X wherein A i1 and A i2 , identical or different are i-mer oligonucleotides with i=3 to 50, where A i1 and Ai 2 are oligomers with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds; R 4 and R 6 , identical or different, are H or a linker, a quencher, a marker, a chromophore group, a fluorophore group, a chemical moiety, a biotin, a hydrophobic chain, a cholesterol derivative, an antigen, a protein, a peptide, a sugar group and a phosphate group; R 5 , different from H, A i and B j , is a chemically stable or cleavable linker between A i and B j ; R 7 and R 8 , identical or different, are different from H and are selected in the group comprising a linker, a quencher, a marker, a chromophore group fluorophore group, a chemical moiety, a biotin, a hydrophobic chain, a cholesterol derivative, an antigen, a protein, a peptide, a phosphate group and a sugar group.