Patent ID: 8568739

Claim:
A recombinant chimeric virus comprising: a first nucleic acid molecule comprising: a 5′ non-coding region from a dengue type 4 virus; a nucleic acid encoding: a C protein from a dengue type 4 virus; and non-structural proteins from a dengue type 4 virus, wherein nonstructural protein NS4B comprises a phenylalanine at amino acid position 112, and nonstructural protein NS5 comprises an alanine at amino acid position 654 and an alanine at amino acid position 655; and a 3′ non-coding region from a dengue type 4 virus, wherein the 3′ non-coding region comprises a deletion of nucleotides 10478-10507; and a second nucleic acid molecule operably linked to the first nucleic acid molecule, the second nucleic acid molecule encoding: a prM protein from a tick borne encephalitis virus; and an E protein from a tick borne encephalitis virus, wherein the E protein comprises an amino acid substitution from wild-type at amino acid position 315 and a tryptophan at amino acid position 240.