Patent ID: 7790401

Claim:
A method for enhancing the bioavailability of a hydrophobic drug in a sample suspected of containing a hydrophobic drug, the method comprising: (a) providing in combination in a medium: (i) the sample wherein the sample is from a biological fluid or a biological tissue and wherein the sample has not been treated to remove lipoproteins and (ii) a bioavailability agent for the hydrophobic drug, wherein the bioavailability agent comprises an ionic detergent comprising a chain of at least 10 carbon atoms or a non-ionic detergent comprising a chain of at least 15 repeating ethylene oxide units or propylene oxide units or a combination of ethylene oxide units and propylene oxide units and wherein the concentration of the bioavailability agent in the medium is sufficient to enhance the bioavailability of the hydrophobic drug by reducing interference from cholesterol and triglycerides, wherein the concentration of the bioavailability agent is about 0.0001% to about 0.5% and (b) incubating the medium under conditions for enhancing the bioavailability of the hydrophobic drug.