Patent ID: 8143600

Claim:
A system for 3D imaging of live cells in an optical tomography system comprising: a microfluidics cartridge including a tube positioned relative to a microscope objective, a conduit loop having a first port coupled to an entrance valve, a second port coupled to an exit valve, a semi-permeable membrane portion, a rotating portion and an imaging window; where the rotating portion is mounted between a first fitting and a second fitting, where the first fitting couples the rotating portion to the entrance valve and the second fitting couples the rotating portion to the exit valve; a rotation mechanism attached to the rotating portion; a microscope objective located to view objects through the imaging window; an axial translation mechanism coupled to the microscope objective; a second conduit interfacing with the semi-permeable membrane, where the second conduit carries nutrients into the conduit loop and waste products out of the conduit loop; at least one radiation source positioned to illuminate the imaging window including a biological object held therein, where the at least one radiation source generates radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm; at least one sensor positioned to sense radiation transmitted through the biological object and the microscope objective; an image processor coupled to receive data from the sensor; and a reconstruction module coupled to the image processor, where the reconstruction module processes the data to form a 3D image of the biological object.