Patent ID: 7824696

Claim:
An isolated or synthetic immunostimulatory oligonucleotide compound comprising an immunostimulatory domain of formula (II): 5′-------X1-X2-Y-Z-X3-X4------3′ (II) wherein Y is a non-natural pyrimidine nucleoside selected from group consisting of 5-hydroxycytosine, N4-alkylcytosine and 4-thiouracil; Z is guanosine, 2′-deoxyguanosine, or a non-natural purine nucleoside; X1 is a naturally occurring nucleoside or an immunostimulatory moiety selected from the group consisting of C3-alkyl linker, 2-aminobutyl-1,3-propanediol linker, and β-L-deoxynucleoside; X2 is a naturally occurring nucleoside or an immunostimulatory moiety that is an amino linker; X3 is a naturally occurring nucleoside or an immunostimulatory moiety that is a nucleoside methylphosphonate; X4 is a naturally occurring nucleoside or an immunostimulatory moiety that is a nucleoside methylphosphonate; and provided that at least one of X1, X2, X3, and X4 is an immunostimulatory moiety.