Patent ID: 7910302

Claim:
A method of making a random array of amplified polynucleotides, said method comprising the steps of: a) providing: i) a surface, which comprises capture probes, wherein said capture probes have free 3′ ends, and ii) a plurality of concatemers disposed on said surface, wherein each concatemer is bound to capture probes at a specific position on said surface and wherein each concatemer comprises multiple copies of a monomer, and said monomer comprises a first interspersed adaptor that is located between a first and second target sequence of a target polynucleotide, wherein said first and second target sequences are contiguous segments of said target polynucleotide; and b) extending said capture probes such that said concatemers disposed on said surface are amplified; wherein the amplification products of said concatemers are attached to the surface at or near the specific position of the concatemer amplified, thereby making said random array of amplified polynucleotides.