Patent ID: 7229551

Claim:
A high performance liquid chromatographic apparatus size-dimensioned to have a flow velocity gradient of 250 microliters per minute or less, said apparatus in which a solvent pump (P 1 ), an injector (I), and a switching valve (V) are connected in this order in one line; and a solvent pump (P 2 ), a switching valve (V), a diffusion promoting device (DU) that comprises a solvent inlet tube and a solvent outlet tube, wherein said device includes at least one feature selected from the group consisting of (ii) having a solvent outlet tube connected to a solvent inlet tube at an acute angle, a right angle, or an obtuse angle and (iii) having a solvent outlet tube connected to a solvent inlet tube by a connecting part having a diameter that is larger than the diameters of the diameters of the solvent inlet and outlet tubes, a separation column (C), and a detector (D) are connected in another line.