Patent ID: 8658376

Claim:
A method of detecting bladder cancer in a human, comprising the steps of: (a) obtaining a urine sample from a human suspected of bladder cancer; (b) forming a precipitate from said urine sample with a chemical compound selected from the group consisting of acetone, trichloroacetic acid, ethanol, methanol/chloroform, and ammonium sulfate; (c) re-suspending said precipitate in a polar solvent to form a solution, said solution has a final volume that is 10-50 fold less than that of said urine sample; (d) concentrating said solution 2-10 fold by filtration; and (e) detecting DEK-2 protein in said concentrated solution using an anti-DEK antibody in a Western blot assay, wherein said detected DEK-2 protein consists of SEQ ID NO: 4, and the presence of said detected DEK-2 protein in said urine sample as compared to a urine sample from a healthy individual is indicative of bladder cancer in said human.