Patent ID: 8741650

Claim:
A method for producing a Sendai virus vector, which comprises the steps Of: expressing a bacteriophage RNA polymerase-encoding DNA under the direct control of a cytomegalovirus enhancer and chicken β-actin promoter-comprising promoter in a virus-producing cell selected from the group consisting of a 293T cell and a BHK-21 cell; transcribing with the RNA polymerase, a DNA that encodes the Sendai virus genome RNA or the complementary strand thereof, and that is operably linked with a recognition sequence of the RNA polymerase in the virus-producing cell; and expressing Sendai virus N (nucleocapsid), P (phospho), and L (large) proteins that form a ribonucleoprotein with the genome RNA under the direct control of the cytomegalovirus enhancer and chicken β-actin promoter-comprising promoter in the virus-producing cell, wherein said method produces at least 1000-fold more of said Sendai virus vector than a method where said cytomegalovirus enhancer and chicken β-actin promoter-comprising promoter is replaced by a cytomegalovirus (CMV) promoter.