Patent ID: 7455990

Claim:
A method for extracting a soluble or membrane associated intracellular recombinant protein of interest (POI) from a bacterial, yeast or fungal cell, the POI being released from the bacterial, yeast or fungal cell, comprising the steps of: (a) providing a bacterial, yeast or fungal cell comprising a soluble or membrane associated intracellular recombinant POI; (b) releasing the recombinant POI from the cell by contacting the cell with a membrane extracting composition comprising a quaternary ammonium compound at a concentration of between 0.05% to 0.6% by weight, under conditions sufficient for the release of the recombinant POI in a soluble form; and, (c) recovering the recombinant POI from the membrane extracting composition; wherein the quaternary ammonium compound is selected from the group consisting of Lauroyl Trimethyl Ammonium Bromide (LTAB), Myristyl Trimethyl Ammonium Chloride (MTAC), CetylTrimethyl Ammonium Chloride (CTAC), Cetrimide, Cetyl Trimethyl Ammonium Bromide (CTAB), Stearoyl Trimethyl Ammonium Chloride (STAC), Stearoyl Trimethyl Ammonium Bromide (STAB), Benzalkonium Chloride (alkyldimethylbenzylammonium chloride), N-Cetylpyridinium Bromide (N-Hexadecylpyridinium bromide), N-Cetylpyridinium Chloride (N-Hexadecylpyridinium chloride), Benzyl Dimethyl Tetradecyl Ammonium Chloride, Benzyl Dimethyl Hexadecyl Ammonium Chloride and a combination of any two or more thereof, and wherein the recombinant POI is a hexose oxidase (HOX) enzyme.