Patent ID: 7602954

Claim:
A method of determining an amount of at least one molecular specie comprising a sample, each molecular specie being indicated by a dye, from an image of the sample captured by an image acquisition device, the image comprising a plurality of pixels, said method comprising: forming a dye space representation of a plurality of dyes, each dye having a corresponding correspondence table comprising a plurality of normalized RGB triplets, the dye space representation having one dimension for each dye and providing a reference model for a combination of the plurality of dyes; comparing each pixel of the image of the sample, the sample being treated by the combination of the plurality of dyes, to the reference model for the combination of the plurality of dyes, each pixel having a color defined by an RGB triplet, so as to determine an optimal combination of normalized RGB triplets from the respective correspondence tables of the dyes producing the color of the respective pixel, the normalized RGB triplets of the optimal combination being identifiable according to the respective dye; and forming an artificial image of the sample, the artificial image corresponding to the sample image, from the normalized RGB triplets for each dye determined from the optimal combination, the artificial image thereby indicating a distribution of the respective dye over the sample image and facilitating determination of the amount of the corresponding molecular specie.