Patent ID: 7671218

Claim:
A fluorescent dye reagent having the formula: wherein Fl is a fluorescent dye component selected from the group consisting of coumarins, benzocoumarins, xanthenes, benzo[a]xanthenes, benzo[b]xanthenes, benzo[c]xanthenes, cyanines, acridines, dipyrrometheneboron difluorides, phenoxazines, benzo[a]phenoxazines, benzo[b]phenoxazines and benzo[c]phenoxazines; L f is a linking group having an attached member selected from the group consisting of a protected or unprotected functional group, a reactive group, a polyfunctional linking moiety, a phosphoramidite moiety and a solid support; the subscript m is an integer of from 0 to 1; P z is a zwitterionic phosphonate group having the formula (a) or a protected phosphonate group having formula (b) or (c): wherein the wavy line indicates the direct attachment to a sp 2 carbon of said fluorescent dye component; L is a linking group; N a is an ammonium ion group; each of R A and R B is independently selected from the group consisting of H and a labile protecting group; R C is selected from the group consisting of H, (C 1 -C 8 )alkyl, aryl, aryl(C 1 -C 4 )alkyl, a labile protecting group or an alkylene linking group having a distal hydroxy or protected hydroxy group; the subscript n is an integer of from 1 to 2; and salts thereof.