Patent ID: 8871067

Claim:
An electrochemical sensor for detecting hydrogen peroxide, comprising: (a) a gold (Au) nanoparticle conjugated with a conducting oxide substrate comprising a conduction oxide; (b) a cytochrome c immobilized on the gold nanoparticle via a linker molecule of 11-mercaptoundecanoic acid (11-MUA); wherein (i) the 11-MUA is covalently bound to the surface of the gold nanoparticle through a thiol group and the cytochrome c is covalently linked to the surface of the gold nanoparticle via an amide bond formed between a carboxylic group of the 11-MUA and an amino group of the cytochrome c, and (ii) the detection limit of the sensor for hydrogen peroxide is 0.3-0.8 μM and the sensor reaches a steady-state current in less than 10 sec.