Patent ID: 8877466

Claim:
A bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity, wherein the bacterial cell has increased succinic acid production relative to a corresponding control bacterial cell, wherein the heterologous polypeptide having isocitrate lyase activity is encoded by a nucleic acid selected from the group consisting of: a. a nucleic acid having the nucleotide sequence of SEQ ID NO: 1; b. a nucleic acid encoding the amino acid sequence of SEQ ID NO: 2; c. a nucleic acid which is at least 98% identical to the nucleic acid of a) or b); and d. a nucleic acid encoding a polypeptide comprising an amino acid sequence which is at least 98% identical to the amino acid sequence encoded by the nucleic acid of a) or b), and wherein the heterologous polypeptide having malate synthase activity is encoded by a nucleic acid selected from the group consisting of: a. a nucleic acid having the nucleotide sequence of SEQ ID NO: 3; b. a nucleic acid encoding the amino acid sequence of SEQ ID NO: 4; c. a nucleic acid which is at least 98% identical to the nucleic acid of a) or b); and d. a nucleic acid encoding a polypeptide comprising an amino acid sequence which is at least 98% identical to the amino acid sequence encoded by the nucleic acid of a) or b).