Patent ID: 7482141

Claim:
A method of producing a vitamin K dependent protein, comprising: a) culturing a cell that comprises: i) a nucleotide sequence encoding a vitamin K dependent protein; ii) a heterologous nucleotide sequence encoding a vitamin K dependent carboxylase; iii) a heterologous nucleic acid comprising a nucleotide sequence selected from the group consisting of: 1) the nucleotide sequence of SEQ ID NO:9; 2) a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:10; 3) a nucleotide sequence having at least 95% identity with the nucleotide sequence of SEQ ID NO:9 and that encodes a vitamin K epoxide reductase (VKOR); 4) a nucleotide sequence that hybridizes with the complement of the nucleotide sequence of (1) or (2) above under stringent conditions as represented by hybridization conditions of 0.5M NaHPO 4 , 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C. and wash conditions of 0.1×SSC/0.1% SDS at 68° C. and that encodes a VKOR; 5) a nucleotide sequence that encodes an amino acid sequence having at least 95% identity with the amino acid sequence of SEQ ID NO:10 and that encodes a VKOR and 6) a nucleotide sequence that differs from the nucleotide sequence of(1), (2), (3), (4) or (5) above due to the degeneracy of the genetic code and that encodes a VKOR; and (iv) vitamin K, under conditions whereby the vitamin K dependent protein is produced; and b) harvesting the vitamin K dependent protein.