Patent ID: 8530233

Claim:
An isolated, circular, double-stranded, inverted dimer (ID) expression vector, consisting of: a first inverted repeat (IR) element having a first end and a second end; a second IR element having a first end and a second end; a first sequence located between said first end of said first IR element and said first end of said second IR element; and a second sequence located between said second end of said first IR element and said second end of said second IR element, wherein said first sequence and said second sequence are fully complementary to each other, each of said first and second sequences comprising a nuclear anchoring element and at least a gene, wherein each of said first and second IR elements comprises two lateral nucleic acid sequences, each being complementary to the other, and a central sequence that is different from each of said two lateral nucleic acid sequences, wherein said expression vector is organized as an inverted dimer (ID) for enhancing gene expression in mammalian cells, wherein said expression vector is capable of transfecting mammalian cells, wherein said expression vector is an episomal vector in transfected mammalian cells, wherein said expression vector comprises pGT04-ID, and wherein said gene encodes a protein which is selected from the group consisting of signal peptide, growth factor, hormone, cytokine, chemokine, neuropeptide, antigen, antibody, enzyme, clotting factor, anti-angiogenic factor, pro-angiogenic factor, transport protein, receptor, ligand, regulatory protein, structural protein, reporter protein, transcription factor, ribozyme, fusion protein, and drug-resistance protein.