Patent ID: 8280141

Claim:
A system for analysis of a biological specimen, comprising: a memory storing an image cube of the specimen obtained by a digital camera arranged as an array of pixels comprising image data at M different wavelengths, where M is an integer greater than 2, the specimen having 1 . . . N fluorophores present in the specimen, at least one of which is a quantum dot; and a network server having access to the memory and having a processing unit executing software instructions for: 1) determining from the image cube coefficients C 1 . . . C N for each pixel, wherein the coefficients C 1 . . . C N are related to the concentrations of the 1 . . . N fluorophores present in the specimen imaged by each pixel; 2) morphologically processing an image constructed from the coefficients C 1 . . . C N to identify biological structures present in the specimen, 3) conducting a quantitative analysis of the specimen including calculating fluorophore concentrations for the biological structures identified in (2) from the coefficients C 1 . . . C N ; and 4) generating display data for display of the results of the quantitative analysis process (3) on a display of a workstation remote from the network server; and a network interface for sending the display data to the workstation.