Patent ID: 8063198

Claim:
A method of removing a silyl protecting group from an oligonucleotide, comprising the step of admixing, under aqueous conditions, an oligonucleotide bearing a silyl protecting group with at least one solvent selected from the group consisting of DMSO, DMF, ethanol, isopropanol, methanol, acetonitrile, and combinations thereof, and a deprotecting reagent selected from the group consisting of pyridine-HF, DMAP-HF (dimethylaminopyridine-HF), urea-HF, TSAF (tris(dimethylamino)sulfonium difluorotrimethylsilane), DAST (diethylamino sulfur trifluoride), polyvinyl pyridine-HF and an amine-HF reagent of formula AA: wherein R 1 is alkyl, aryl, heteroaryl, aralkyl or heteroaralkyl; R 2 is alkyl, aryl, heteroaryl, aralkyl or heteroaralkyl; R 3 is aryl or heteroaryl; and n is 1 to 20.