Patent ID: 8859846

Claim:
A method of obtaining a doubled haploid maize plant, said method comprising: (a) pollinating silks of a maize ear with a maize inducer line to produce at least one diploid maize embryo and at least one haploid maize embryo; (b) isolating said haploid maize embryo between 4-21 days after step (a), wherein said at least one haploid maize embryo is distinguished from the diploid maize embryos via expression of a marker; (c) contacting said haploid maize embryo with a chromosome doubling agent to produce at least one doubled haploid maize embryo cell; (d) culturing said doubled haploid maize embryo cell on a non-callus promoting medium; and (e) generating a doubled haploid maize plant from said doubled haploid maize embryo cell.