Patent ID: 8841126

Claim:
A method of transferring a foreign gene into a target cell using a retroviral vector, the method comprising the following steps (a) to (e): (a) immobilizing a retrovirus-binding substance on an inner surface of a bag for cell culture; (b) injecting a liquid containing a retroviral vector carrying a foreign gene into the bag obtained by the step (a), followed by incubation with shaking at a temperature of less than 25° C. for 8 hours to 48 hours to obtain a bag to which the retroviral vector is bound; (c) adding a target cell into the bag obtained by the step (b), followed by incubation for 2 to 8 hours; (d) turning the bag obtained by the step (c) upside down; and then (e) incubating the bag obtained by the step (d) while upside down.