Patent ID: 7264805

Claim:
A preparation of immunoreactive antibody for therapeutic or diagnostic use, wherein the immunoreactive antibody is monoclonal antibody BW250/183 that binds specifically to CD 66 wherein the monoclonal antibody is obtained by culturing hybridoma cells secreting said antibody in a suitable culture media in a fluidized reactor, harvesting the antibody from the culture medium, purifying said antibody by means of one step single column protein A affinity chromatography, determining the percentage of immunoreactive antibody in the presence of an excessive amount of CD66 antigen, and calculating the percentage of immunoreactive antibody that binds specifically to CD66 using a modified Lindmo test according to the formula: % immunoreactive (IR)=100%−[100%×(monoclonal antibody concentration in supernatant/monoclonal antibody-starting concentration)] wherein the preparation having greater than 90 percent monoclonal antibody immunoreactive to CD66.