Patent ID: 8198037

Claim:
A method for determining a protein activation state profile in a single cell comprising: contacting said cell with a first phosphorylation state-specific antibody specific to a phosphorylated isoform of a first protein within the MAPK (mitogen activated protein kinase), AKT (homolog of V-akt murine thymoma viral oncogene), STAT (signal transducers and activators of transcription), NFkB (nuclear factor kappa B), PKC (protein kinase C) or WNT (Wingless/Int) signaling pathways and a second phosphorylation state-specific antibody specific to a phosphorylated isoform of a second protein within the MAPK, AKT, STAT, NFkB, PKC or WNT signaling pathways, wherein said first and second phosphorylation state-specific antibodies are distinguishably detectable; and determining a protein activation state profile in said single cell by simultaneously detecting the binding or absence of binding of said first and second phosphorylation state-specific antibodies to said first and second proteins in said single cell.