Patent ID: 7985731

Claim:
An oligosaccharide chain added glucagon-like peptide-1 (GLP-1) peptide having GLP-1 activity, wherein one amino acid is substituted with an oligosaccharide chain added amino acid, wherein said oligosaccharide chain is not an O-linked oligosaccharide chain in GLP-1; wherein said oligosaccharide chain added GLP-1 peptide is: (a) an oligosaccharide chain added GLP-1 peptide having one amino acid further added to the C terminal (position 37) of GLP-1, wherein said added amino acid is substituted with an oligosaccharide chain added amino acid; (b) an oligosaccharide chain added GLP-1 peptide having one amino acid further added to the N terminal (position 7) of GLP-1, wherein said added amino acid is substituted with an oligosaccharide chain added amino acid; or (c) an oligosaccharide chain added GLP-1 peptide wherein one or more amino acid site(s) selected from positions 18, 20, 22, 26, 30, 34 and 36 of GLP-1 is substituted with an oligosaccharide chain added amino acid; wherein: said oligosaccharide chain added amino acid is oligosaccharide chain added Asn; in said oligosaccharide chain added amino acid, the oligosaccharide chain is linked to the amino acid without a linker; said oligosaccharide chain consists of four or more sugars; and said oligosaccharide chain is selected from the group consisting of disialo, monosialo, asialo, diGlcNAc and dimannose oligosaccharide chains.