Patent ID: 7312044

Claim:
A process for determining whether a first agent inhibits dissociation of FKBP12 from the type 1 ryanodine (RyR1) receptor, comprising: (a) separately contacting (i) skeletal muscle cells expressing the RyR1 receptor or (ii) sarcoplasmic reticulum or endoplasmic reticulum from an extract from such cells, with (1) both the first agent and a second agent known to cause dissociation of FKBP12 from the RyR1 receptor and with (2) only such second agent, under conditions suitable for dissociation of FKBP12 binding protein from the RyR1 receptor in the presence of such second agent; and (b) measuring the extent of dissociation of FKBP 12 from the RyR1 receptor in the skeletal muscle cells in the presence of only the second agent and in the presence of both the fist and second agents, a smaller extent of dissociation of FKBP12 from the RyR1 receptor in the presence of both the first agent and the second agent than in the presence of only the second agent indicating that the first agent inhibits the extent of dissociation of FKBP12 from the RyR1 receptor, so as to thereby determine whether the first agent inhibits dissociation of FKBP12 from the type 1 ryanodine (RyR1) receptor.