Patent ID: 8778842

Claim:
A composition for nucleic acid immobilization and analysis, comprising: (1) a nucleic acid-containing biological sample, and (2) a lysis buffer comprising a synthetic copolymer, wherein the synthetic copolymer comprises a plurality of a first hydrophobic monomer, a plurality of a second monomer, and a plurality of an amino group-containing component, wherein (i) the first hydrophobic monomer is (a) styrene or (b) an alkene or alkapolyene having 2 to 8 carbon atoms, (ii) the second monomer provides at least one functionality to which the amino group-containing component is directly or indirectly covalently linked, wherein the second monomer is selected from the group consisting of maleic anhydride, acrylic acid, methacrylic acid, 4-hydroxy-styrene, and 4-vinyl-benzaldehyde, and (iii) the amino group-containing component is directly or indirectly covalently linked to the second monomer, wherein the amino group-containing component is selected from the group consisting of 2-(diisopropylamino)-ethylamine, N,N-diethyldiethylenetriamine, 1-(2-aminoethyl)pyrrolidine, 1-amino-4-methylpiperazine, N,N,2,2,-tetramethyl-1,3,-propanediamine, 3-(dibutylamine)propylamine, Girard's reagent T, choline chloride, 2-(2-diethylaminoethylamino)-ethanol, 2-{[2-(dimethylamino)ethyl]methylamino}ethanol, 1,3-bis-(diethylamino)-2-propanol, a-(diisopropylamino)ethanol, 2-[2-(dimethylamino)ethoxy]ethanol, 4-diisobytylamino-1-butanol, 6-dipropylamino-1-hexanol, and diethanolamine, and wherein the synthetic copolymer is capable of binding to a hydrophobic surface and a nucleic acid.