Patent ID: 7794947

Claim:
A method of detecting and quantifying polypeptides in a sample, comprising: (a) identifying a set of peptides corresponding to polypeptides of interest in a sample; (b) chemically synthesizing said set of peptides; (c) synthesizing isotopically labeled forms of said set of peptides to generate peptide standards; (d) generating a plurality of binding agents for said set of peptides; (e) immobilizing said plurality of binding agents to a solid support in an array format; (f) cleaving polypeptides in a test sample to generate cleaved test sample peptides corresponding to said peptide standards; (g) adding a predetermined amount of said peptide standards to said cleaved test sample; (h) contacting said cleaved test sample containing peptide standards with said array of immobilized binding agents; (i) washing said array to remove unbound peptides, thereby retaining affinity captured peptides comprising cleaved test sample peptides and standard peptides; (j) detecting the presence of said affinity captured peptides using mass spectrometry; and (k) quantifying the amount of said test sample peptides by determining the ratio of cleaved test sample peptides to corresponding standard peptides.