Patent ID: 7211420

Claim:
A process for oxidizing a substrate wherein said substrate is a limonene or pinene or a cyclic sesquiterpene, wherein said limonene, pinene or cyclic sesquiterpene is optionally substituted by an alkyl of 1 to 6 carbons or an alkenyl of 1 to 6 carbons, and wherein the process comprises: oxidizing said substrate with a mutant haem-containing P450 enzyme, wherein said mutant enzyme is a mutant of a P450 BM-3 comprising the amino acid sequence of SEQ ID NO:24 and said mutant enzyme only has mutations at one or more of the following amino acid positions: 42, 51, 181, 263, 264 and 354 and wherein said mutant enzyme has a higher oxidation activity towards the substrate which is being oxidized than the enzyme having the sequence SEQ ID NO:24, and wherein at least one of said mutations is a substitution of an amino acid by another amino acid.