Patent ID: 7713705

Claim:
A method, comprising: contacting a test sample obtained from a subject with a test surface of a diagnostic device, wherein the test surface is within a capillary space of the diagnostic device and comprises a plurality of discrete addressable locations, each of the discrete addressable locations comprising discrete immobilized particles comprising antibodies bound thereto for binding a marker, the diagnostic device comprising immobilized particles at discrete addressable locations for detecting each of B-type natriuretic peptide or a marker related to B-type natriuretic peptide, D-dimer, and at least one cardiac troponin form selected from the group consisting of free cardiac troponin I, free cardiac troponin T, cardiac troponin I in a complex comprising one or both of troponin T and troponin C, cardiac troponin T in a complex comprising one or both of troponin I and troponin C, total cardiac troponin I, and total cardiac troponin T; detecting the amount of each of B-type natriuretic peptide or a marker related to B-type natriuretic peptide, D-dimer, and at least one cardiac troponin form selected from the group consisting of free cardiac troponin I, free cardiac troponin T, cardiac troponin I in a complex comprising one or both of troponin T and troponin C, cardiac troponin T in a complex comprising one or both of troponin I and troponin C, total cardiac troponin I, and total cardiac troponin T; and determining the presence or absence of each of myocardial infarction, pulmonary embolism, and congestive heart failure in said subject based on the results obtained from said analyzing step; wherein detecting an amount of at least one cardiac troponin form above a threshold level rules in myocardial infarction; further wherein when a detected amount of at least one cardiac troponin form is below a threshold level, and the detected amount of D-dimer is above a threshold level, pulmonary embolism and congestive heart failure are distinguished by comparing the amount of D-dimer to the amount of BNP or a marker related thereto detected in said test sample.