Patent ID: 7261544

Claim:
A method for purifying AAV vector particles from an AAV preparation comprising AAV vector particles and AAV empty capsids, to provide an AAV product substantially free of AAV empty capsids, said method comprising: (a) providing a host cell comprising AAV vector particles; (b) lysing said host cell to obtain a crude cell lysate comprising AAV vector particles and AAV empty capsids; (c) applying said crude cell lysate to a cation exchange chromatograhphy column under conditions whereby said AAV vector particles and said AAV empty capsids bind the column; (d) eluting said AAV vector particles and said AAV empty capsids under non-separating conditions to provide an AAV preparation comprising AAV vector particles and AAV empty capsids; (e) applying the AAV preparation from (d) to an anion exchange chromatography column under conditions whereby said AAV vector particles and said AAV empty capsids bind the column; (f) adding a salt buffer to the column from (e) under conditions whereby AAV empty capsids are eluted and AAV vector particles remain bound to the column; (g) adding a buffer with a greater salt concentration relative to the buffer added in (f) to the column from (f) under conditions whereby AAV vector particles are eluted; (h) collecting eluted fractions from (g) that comprise AAV vector particles to provide an AAV product substantially free of AAV empty capsids.