Patent ID: 7642069

Claim:
A process for the production of intracellular streptokinase using E. coli PSK4 (MTCC 5120), said process comprises steps of: a. adapting the strain of E. coli PSK4 having Accession No. MTCC 5120 in a growth medium having carbon source, basal salts and trace metals to prepare seed culture; b. adding the seed culture of step (a) to a fermentation medium and fermenting the same for a period of 8 hours to 12 hours; c. adding an inducer to the fermentation medium of step (b) and fermenting the medium for another 3 hours to 5 hours to produce streptokinase-containing inclusion bodies, and d. harvesting the E. coli , lysing the E. coli , and resolubilizing the inclusion bodies of step (c) to obtain the streptokinase.