Patent ID: 7947289

Claim:
A composition comprising a first effector component of a multimeric bacterial protein toxin, the first effector component comprising at least a first modified monomer and a second modified monomer, wherein said first and second modified monomers form a heterooligomer, wherein said first and second modified monomers are different, and wherein said first and second modified monomers are generated by modification of a first and second unmodified monomers, respectively, the modification of each of said first and second unmodified monomers meeting at least two of the following criteria: (a) substitution of a native cell-recognition domain in said first or second unmodified monomer for a non-native cell-recognition domain; (b) substitution of a native proteolytic activation site in said first or second unmodified monomer for a non-native proteolytic activation site; (c) at least one mutation made to said first unmodified monomer, whereby said first modified monomer can form a heterooligomer with said second modified monomer only; and (d) at least one mutation made to each of said first and second unmodified monomers, whereby a second effector component can bind only at a site formed by the interaction of said first modified monomer and said second modified monomer.