Patent ID: 7815788

Claim:
A method for calibrating a biosensor for the amperometric determination of creatinine in biological liquids, wherein the biosensor is integrated and operated in a device and has one electrode system each for measuring the concentrations of creatinine and creatine, with the electrode system for measuring the concentration of creatinine comprising creatininase, creatinase and sarcosine oxidase and the electrode system for measuring the concentration of creatine comprising creatinase and sarcosine oxidase, the method comprising: mixing an acidic creatinine solution with an alkaline buffer to form a creatinine calibration solution; calibrating the electrode system for measuring the creatine concentration with a creatine calibration solution wherein creatinine and creatine are at a thermodynamic equilibrium; and calibrating the electrode system for measuring creatinine concentration with the creatinine calibration solution, wherein the result of the electrode system for measuring the concentration of creatinine is corrected by the result of the electrode system for measuring the concentration of creatine.