Patent ID: 7833762

Claim:
A method of producing L-lysine comprising: A) culturing an Escherichia coli in a medium, and B) collecting the L-lysine from the medium or the Escherichia coli, wherein the Escherichia coli is an L-lysine-producing Escherichia coli , and wherein the Escherichia coli has been modified to enhance acetyl-CoA synthetase activity by a method selected from the group consisting of: a) increasing the copy number of the acs gene which encodes the acetyl-CoA synthetase, b) modifying an expression regulatory sequence of said gene, and c) combinations thereof, and wherein the acs gene is selected from the group consisting of: (i) a DNA comprising the nucleotide sequence of SEQ ID NO: 3 and (ii) a DNA that hybridizes with a nucleotide sequence which is complementary to the nucleotide sequence of SEQ ID NO: 3 under stringent conditions of washing in 0.1×SSC, 0.1% SDS at 60° C., and wherein said DNA encodes a protein with acetyl-CoA synthetase activity.