Patent ID: 8592174

Claim:
A method for the detection in microorganisms of a peptidase activity comprising the following steps: a) providing a detecting and/or identifying medium comprising a compound of the following formula (I): wherein: Y 1 is a peptide, H or an alkyl; W 1 , W 2 , W 3 and W 4 are independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl, carboxyl esters or carboxyl amides or any combination thereof; n=0, 1 or 2; wherein if V is N or N + R then U is CZ 6 ; R is H, alkyl, aralkyl, aryl, alkanoyl or alkylsulfonyl; Z 1 , Z 2 , Z 3 , Z 4 and Z 5 are independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulfonyl, including the carboxyl or sulfonyl esters or amides, and salts thereof, b) inoculating the medium with a biological sample to be tested, c) leaving to incubate, and d) detecting the presence of at least one peptidase activity; wherein the compound is also an indicator of pH variation.