Patent ID: 8206905

Claim:
A method of quantitation of inorganic pyrophosphate in a sample, comprising: a) obtaining at least one sample suspected of containing inorganic pyrophosphate molecules; b) adding pyrophosphate detection solution to said sample, wherein said pyrophosphate detection solution utilizes ATP sulfurylase, 5′-phosphosulphate (APS), luciferin-luciferase and inorganic pyrophosphatase (PPase); c) detecting intensity of luminescent light vs, time from said sample with said pyrophosphate detection solution added; d) measuring amplitudes of the rising and decaying components of said luminescent intensity vs. time; e) calculating the amount of inorganic pyrophosphate and ATP present in said sample, wherein the amount of pyrophosphate is proportional to the rising component of luminescent intensity vs. time and the amount of ATP is proportional to the difference between the rising and decaying component of the luminescent signal.