Patent ID: 7858350

Claim:
A host microbial organism transformed with nucleic acids encoding enzymes of a 1,4-butanediol (BDO) pathway, said microbial organism comprising exogenous nucleic acids encoding BDO pathway enzymes expressed in a sufficient amount to produce BDO, said BDO pathway enzymes comprising: a) 3-hydroxybutyryl-CoA dehydrogenase, said enzyme classified as EC 1.1.1.a and converting acetoacetyl-coenzyme A (CoA) to 3-hydroxybutyryl-CoA; b) 3-hydroxybutyryl-CoA dehydratase, said enzyme classified as EC 4.2.1.a and converting 3-hydroxybutyryl-CoA to crotonoyl-CoA; c) vinylacetyl-CoA A-isomerase, said enzyme classified as EC 5.3.3.3 and converting crotonoyl-CoA to vinylacetyl-CoA; d) 4-hydroxybutyryl-CoA dehydratase, said enzyme classified as EC 4.2.1.a and converting vinylacetyl-CoA to 4-hydroxybutyryl-CoA; and e) 4-hydroxybutyryl-CoA reductase (alcohol forming), said enzyme classified as EC 1.1.1.c and converting 4-hydroxybutyryl-CoA to 1,4-butanediol; or 4-hydroxybutyryl-CoA reductase, said enzyme classified as EC 1.2.1.b and converting 4-hydroxybutyryl-CoA to 4-hydroxybutanal, and 1,4-butanediol dehydrogenase, said enzyme classified as EC 1.1.1.a and converting 4-hydroxybutanal to 1,4-butanediol.