Patent ID: 7384757

Claim:
A method for identifying modulators of human acetyl coenzyme A carboxylase (ACC) comprising: a) adding a control mixture to a control reaction vessel comprising a solid support, a scintillant and a linking moiety; wherein said control mixture excludes exogenous malonyl Co A and comprises purified human ACC, purified recombinant fatty acid synthase (FAS), magnesium, adenosine tri-phosphate (ATP), NaHCO 3 , citrate, dimethyl sulfoxide (DMSO), and reduced adenine nicotinamide dinucleotide phosphate (NADPH); b) adding a test mixture to a test reaction vessel comprising a solid support, a scintillant and a linking moiety; wherein said test mixture excludes exogenous malonyl Co A and comprises purified human ACC, purified recombinant fatty acid synthase (FAS), magnesium, adenosine tri-phosphate (ATP), NaHCO 3 , citrate, a test agent having unknown biological activity, DMSO, and reduced adenine nicotinamide dinucleotide phosphate (NADPH); c) adding tritiated acetyl coenzyme A ( 3 H-acetyl CoA) to said control reaction vessel and said test reaction vessel and incubating said vessels for an incubation period; d) obtaining a tritiated product in said control and test reaction vessels; e) measuring the radioactivity associated with said tritiated product in the control reaction vessel and test reaction vessel; and f) comparing the radioactivity levels in the control reaction vessel and test reaction vessel whereby a difference in radioactivity in the test reaction vessel indicates that said test agent is a modulator of ACC activity.