Patent ID: 8759031

Claim:
A method for incorporating an aminomalonyl into a hybrid polyketide molecule, comprising adding and reacting aminomalonyl-acyl carrier protein (AM-ACP) in a cell-free system with (a) an isolated polypeptide comprising an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:6 and an amino acid sequence that is at least 95% identical to SEQ ID NO:6, wherein said isolated polypeptide has the biological activity of catalyzing the incorporation of an ethanolamine subunit from AM-ACP into a polyketide; and (b) a second isolated polypeptide comprising (i) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:7 and an amino acid sequence that is at least 95% identical to SEQ ID NO:7, whereby the second isolated polypeptide has ketosynthase activity, (ii) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:8, whereby the second isolated polypeptide has ketoreductase activity, and (iii) an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO:9 and an amino acid sequence that is at least 95% identical to SEQ ID NO:9, whereby the second isolated polypeptide has acyl carrier protein activity, wherein said isolated polypeptides of (a) and (b) incorporate the aminomalonyl into the hybrid polyketide molecule.