Patent ID: 8367382

Claim:
A method for producing 4-hydroxyisoleucine or a salt thereof comprising: A) subjecting isoleucine or a salt thereof to a hydroxylation reaction in the presence of a hydroxylase derived from a microorganism to produce a reaction product, and B) isolating 4-hydroxyisoleucine or a salt thereof from the reaction product, wherein the microorganism is Bacillus thuringiensis , and wherein the hydroxylase comprises: (a) the ability to catalyze the production of (2S,3R,4S)-4-hydroxy-L-isoleucine by hydroxylation of L-isoleucine, wherein Fe 2+ and 2-oxoglutaric acid are essential for said ability; and (b) a molecular weight as measured by SDS-PAGE of about 29±2.0 kDa.