Patent ID: 7271008

Claim:
A device for determining quality of a reagent used in an assay, comprising: a) a target of a secondary antibody to be used in said assay; said target being bound on a first plurality of spatially defined sites an a substrate, and each of said first plurality of spatially defined sites having a different amount of said target; b) a secondary antibody conjugate comprising said secondary antibody and a ligand to be used in said assay; said secondary antibody conjugate being bound by a first linking moiety on a second plurality of spatially defined sites on said substrate, each of said second plurality of spatially defined sites having a different amount of said secondary antibody conjugate; and c) an enzyme conjugate comprising an enzyme to be used in said assay and a binding partner specific to said ligand; said enzyme conjugate being bound by a second linking moiety on a third plurality of spatially defined sites on said substrate, each of said third plurality of spatially defined sites having a different amount of said enzyme conjugate.