Patent ID: 7869958

Claim:
A method of improving binding or activity of an agonist or antagonist of a corticotropin-releasing hormone receptor 2β extracellular domain (CRFR2β-ECD1) comprising: a) employing the structural coordinates of mouse CRFR2β-ECD1 as set forth in Table 3 to generate a three-dimensional model in silico; b) utilizing the model as generated in step (a) to analyze the interaction(s) of known antagonists or agonists of human CRFR2β-ECD1 to amino acids 67-69, 90-93, 102, 103 and 112-116 or combinations thereof wherein said amino acids correspond to the amino acid positions in Table 3, d) ascertaining from step (b) where or how to modify said known antagonists or agonists so as to improve the binding or fit and modifying said agonists or antagonists thereby forming new/modified agonists or antagonists; e) providing said new/modified agonists or antagonists; and f) assessing the binding and/or activity of said modified/new agonists or antagonists with CRFR2β-ECD1 by contacting said new agonists or antagonists with a CRFR2β-ECD1 polypeptide.