Patent ID: 7348160

Claim:
A method for the detection, characterization and qualitative and/or quantitative determination of the activity of a phosphoamidase PHP1 enzyme, comprising hydrolyzing a phospho-ester bond (P—O) of at least one of the substrates which is FDP (fluorescein diphosphate), DDAO (9H-(1,3-dichloro-9,9-dimethyl-acridin-2-one-7-yl)phosphate), DiFMUP (6,8-difluoro-4-methylum-belliferyl phosphate), ELF®39 phosphate (2-(2′-phosphophenyl)-4-(3H)-quinazolinone) or ELF®97 phosphate (2-(5′-chloro-2′-phosphophenyl)-6-chloro-4-(3H)-quinazolinone) and correlating the resultant hydrolysis level with the phosphoamidase activity of said enzyme.