Patent ID: 8790881

Claim:
A method for measuring immunoglobulin G-mediated complement activation, comprising the following steps: a) preparing a sample A of immunoglobulin G (IgG) and a sample B comprising natural serum, said natural serum optionally being diluted in a dilution buffer; b) mixing sample A with sample B at a ratio (amount of IgG in sample A in grams): (volume of natural serum in sample B in liters) of between 30 and 75, at a temperature of between 2° C. and 6° C., and subsequently incubating the resulting reaction mixture at a temperature of between 35° C. and 40° C. for a period of between 30 minutes and 2 hours; c) cooling the reaction mixture obtained at the end of step b) to a temperature of between 0° C. and 4° C. in the presence of EDTA; and d) measuring the amount of C5a fragment in the cooled reaction mixture obtained in c).