Patent ID: 8613845

Claim:
A column separation system comprising: a capillary having an upstream inlet end and a downstream terminus end; an electrically conductive hollow needle having an inner wall defining an internal tapered chamber, the needle having an upstream opening and a downstream exit orifice, the downstream exit orifice axially opposed to the upstream opening, the internal tapered chamber dimensioned and configured to have a diameter decreasing from a larger diameter at the opening to a smaller diameter at the exit orifice to form a taper on the inner wall in a longitudinal direction from the opening to the exit orifice, the larger diameter at the opening dimensioned and configured to slidably accept the terminus end of the capillary, the capillary longitudinally inserted into and mounted within the internal tapered chamber to a distance whereby the terminus end of the capillary in operational state abuts the inner wall of the needle at the taper; and a flow-through micro-reservoir is formed between the terminus end of the capillary and the downstream exit orifice.