Patent ID: 8188260

Claim:
A ribozyme catalyzing tRNA acylation having a structure consisting of an RNA sequence represented by the general formula below: P1-CCGC(N 1 ) 1 (N 1 ) 2 . . . (N 1 ) p -P2-(N 2 ) 1 (N 2 ) 2 . . . (N 2 ) q GCG(N 3 ) 1 (N 3 ) 2 AGG N wherein P1 and P2 represent a domain consisting of any RNA sequence capable of having a stem-loop structure; (N 1 ) 1 -(N 1 ) p each independently represent any monoribonucleotide of U, C, A or G; p represents 3 or 4; (N 2 ) 1 -(N 2 ) q each independently represent any monoribonucleotide of U, C, A or G; q represents 5 or 6; (N 3 ) 1 -(N 3 ) 2 each independently represent any monoribonucleotide of U, C, A or G; U represents an uracil nucleotide; C represents a cytosine nucleotide; A represents an adenine nucleotide; G represents a guanine nucleotide; and N represents a monoribonucleotide complementary to A or G; wherein the ribozyme recognizes a tRNA via the 3′-terminal GG N motif to bind the tRNA and said GG N motif is complementary to a nucleotide sequence at positions 75-73 at the 3′ end of the tRNA binding the ribozyme, and wherein the structure of the ribozyme catalyzing tRNA acylation consists of an RNA sequence represented by formula (I) or (II) below: (SEQ ID NOS: 1 and 2) P1-CCGCGGC-P2-GAUUAGCGUUAGG N (I) (SEQ ID NOS: 3 and 4) P1-CCGCAUC-P2-UACAUGGCGUUAGG N (II).