Patent ID: 7291470

Claim:
A method for detecting Shigella sonnei in a solution sample, which comprises the following steps: (a) incubating said sample with primers IS1SS and IS1SR3 in a PCR reaction solution whereby a PCR reaction takes place which amplifies nucleic acids to produce a 369 base pair amplification product, wherein the primer IS1SS consists of SEQ ID NO: 1 and the primer IS1R3 consists of SEQ ID NO:2; (b) detecting the presence of a 369 base pair amplification product; (c) cutting the 369 base pair amplification product by restriction enzyme BstEII; (d) detecting the presence of the DNA fragments of the 78 base pair and 291 base pair; wherein the presence of said DNA fragments are indicative of the presence of Shigella sonnie in the sample.