Patent ID: 8771953

Claim:
An in vitro method comprising the following steps of: a) obtaining a biological sample containing mammal cells wherein said mammal cells are cell lines of human origin which exhibit a regular and constitutive expression of the editing enzymes ADAR1a, ADAR1b and ADAR2 and of the serotonin 2C receptor (5HTR2C); b) contacting said mammals cells with the compound to be tested; c) determining in the cellular RNA extract of said biological sample: the editing profile giving the mean proportion of each identified isoform of the 5-HT2CR mRNA measured in the cellular RNA extract, and the quantitative expression of said editing enzymes ADAR1a, ADAR1b and ADAR2; d) comparing the results obtained in step c) between said treated cells with the compound to be tested and non treated control cells, and e) determining the potential toxicity or side-effects of a test compound after its administration in a patient, wherein in step a), said mammal cells are from the human neuroblastoma SH-SY5Y cell line.