Patent ID: 7407654

Claim:
An isolated antibiotic GE 81112 Factor A comprising the following characteristics: A) Mass spectrum recorded from a 0.01 mg/ml solution in acetonitrile: water 50:50 giving a monoprotonated ion at 644 m/z on a Thermofinnigan® LCQ deca mass spectrum instrument under the following electrospray conditions: Spray Voltage: 4.7 kV; Capillary temperature: 250° C.; Capillary Voltage 9V; Infusion mode 5 μl/min; B) Infrared Spectrum recorded as a nujol mull with an IFS-48® Fourier Transform infrared spectrophotometer showing the following main bands (cm −1 ): 3356; 2956; 2855; 2256; 2128; 1663; 1596; 1455; 1378; 1345; 1123; 1050; 1026; 1002; 825; 764; C) 1 H-NMR spectrum recorded at 600 MHz at 25° C. in DMSO-d 6 , comprising at least one drop of trifluoroacetic acid, showing the following 1 H signals: Chemical Shift (ppm) Multiplicity 1.52 br d 1.57-1.67 m 1.81 br d 1.88 m 2.87 dd 2.90 m 3.12 dd 3.15 m 3.65 m 3.83 m 3.94 br d 4.40 br s 4.46 m 4.50 dd 4.70 M 5.06 D 6.46 Br 6.94 S 7.29 S 7.99 D 8.17 D 8.30 M 8.57 br d 8.95 D 9.06 br d 14.09 Br 14.27 Br — — D) 13 C-NMR spectrum recorded at 150 MHz, at 25° C. in DMSO-d 6 , comprising at least one drop of trifluoroacetic acid, showing the following 13 C signals Chemical Shift (ppm) Multiplicity 15.9 t 27.19 t 28.14 t 35.31 t 42.86 t 50.13 d 51.30 d 56.86 d 59.71 d 64.07 d 65.11 d 66.88 d 67.76 T 116.83 d 118.49 d 128.41 s 129.03 s 133.65 d 139.66 s 156.73 s 167.39 s 170.16 s 171.29 s 171.65 s E) Retention time 14.1 min, determined by using an analytical HPLC pump Shimadzu® LC10-AS equipped with a chromatographic column Altima® RP18, 5 μm, 250×4.6 mm i.d column; isocratic elution performed at 1 ml/min. flow rate, with 40 mM ammonium formate buffer, brought to pH 4.5 by addition of formic acid; UV detection at 230 nm; F) possesses acid functions and basic functions; and G) inhibits the growth of Moraxella catarrhalis ; inhibits the growth of Escherichia coli and Bacillus subtilis when these bacteria are cultivated in minimal media; shows marginal activity against Streptococcus pneumoniae ; does not inhibit growth of Candida albicans , and wherein the antibiotic GE 81112 Factor A also includes one or more corresponding salts formed with acids and/or bases.