Patent ID: 6870075

Claim:
A method for producing a non-transgenic, herbicide resistant or tolerant plant comprising: (a) introducing into plant cells a recombinagenic oligonucleobase with a targeted mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses a EPSPS protein that is mutated at one or more amino acid positions, said positions selected from the group consisting of Leu 173 , Ala 179 , Met 180 , Arg 181 , Ser 98 , Ser 255 and Leu 198 in the Arabidopsis EPSPS protein or at an analogous amino acid residue in an EPSPS paralog; (b) identifying a plant cell having substantially normal growth as compared to a corresponding wild-type plant cell in the presence of glyphosate; and (c) regenerating a non-transgenic herbicide resistant or tolerant plant having a mutated EPSPS gene from said plant cell.