Patent ID: 8617853

Claim:
A method for reducing the substrate 2-[3-[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-oxopropyl]benzoic acid methyl ester of formula (I) to the product (S,E)-methyl 2-(3-(3-(2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-hydroxypropyl)benzoate of formula (II), or for reducing the substrate 2-[2-[3-[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-oxopropyl]phenyl]-2-propanol of formula (III) to the product (S,E)-1-(3-(2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-(2-(2-hydroxypropan-2-yl)phenyl)propan-1-ol of formula (IV), which comprises contacting the substrate of formula (I) with a ketoreductase polypeptide which comprises an amino acid sequence that is at least 90% identical to a reference sequence based on SEQ ID NO: 2, 4, or 114 and having at position 190 a proline, at position 195 an arginine, and at position 202 a tryptophan, under reaction conditions suitable for reducing or converting the substrate to the product of formula (II).