Patent ID: 8071739

Claim:
A polynucleotide molecule of formula PNâ€”OZ, wherein Z is selected from -allyl, â€”CRâ€² 2 O-allyl, â€”CRâ€² 2 NRâ€³(allyl), â€”CRâ€² 2 NH(allyl), â€”CRâ€² 2 N(allyl) 2 or -CRâ€² 2 -â€”S-allyl, PN is a 3â€²-terminal nucleotide of said polynucleotide; each Râ€² is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy, amido group or two Râ€² taken together represent an alkylidene group â•C(Râ€²â€³), wherein Râ€²â€³ is selected from hydrogen, halogen, and alkyl; Râ€³ is a removable protecting group; and said polynucleotide further comprises a detectable label linked by a linker.