Patent ID: 7713725

Claim:
A method for producing a protein of interest encoded by a gene under the control of an inducible promoter comprising the steps of: (a) Generating a first mixture comprising between about 5% to about 75% glucose and a cellulase preparation selected from the group consisting of (1) whole cellulase composition and (2) beta-glucosidase enriched cellulase composition to give a first mixture, the beta-glucosidase activity in said first mixture being about 1.5 to about 180 IU/ml; (b) Incubating the first mixture at a temperature and for a sufficient time to produce an inducing feed composition comprising sophorose in a concentration ranging from 2 g/L to 25 g/L, gentiobiose in a concentration ranging from 35 g/L to 60 g/L, and glucose; and (c) Culturing a cell comprising a nucleotide sequence encoding a protein of interest under the control of a sophorose-inducible promoter or a gentiobiose-inducible promoter with said inducing feed composition, wherein said inducing feed has not been subjected to a purification step, in an amount effective to induce the production of said protein of interest.