Patent ID: 7709604

Claim:
A composition comprising: a eukaryotic translation system; an orthogonal aminoacyl-tRNA synthetase (O-RS) comprising at least 90% identity to SEQ ID NO: 2 and comprising a proline residue at a position corresponding to position 144 of SEQ ID NO: 2, wherein the O-RS aminoacylates a reference O-tRNA of SEQ ID No: 3 with a 5-substituted tryptophan analog or 5-hydroxy-L-tryptophan (5-HTTP) when the reference O-tRNA and the 5-substituted tryptophan analog or 5-HTTP are present in the translation system; and, an orthogonal tRNA (O-tRNA) comprising: at least 90% identity to SEQ ID NO: 3, wherein the O-tRNA is aminoacylated with the 5-HTTP by a reference O-RS of SEQ ID NO: 2 when the reference O-RS and the 5-substituted tryptophan analog or 5-HTTP are present in the translation system.