Patent ID: 8440463

Claim:
A method for predicting a risk of developing chronic renal insufficiency in a subject suffering from diabetes mellitus type I, the method comprising: (a) contacting a sample from the subjects with a ligand specific for placental growth factor (PLGF); (b) calculating an amount of PLGF in the sample based contacting step (a); (c) contacting a sample from the subject with a ligand specific for soluble Flt-1 (sFlt-1); (d) calculating an amount of sFlt-1 in the sample based contacting of step (c) comparing the calculated amount of PLGF and the calculated amount of sFlt-1 with a reference amount of PLGF and a reference amount of sFlt-1, respectively; and predicting a risk of developing chronic renal insufficiency if the calculated amount of PLGF is greater than a reference amount of PLGF between 12 and 15 pg/ml and the calculated amount of sFlt-1 is greater than a reference amount of sFlt-1 between 90 and 120 pg/ml.