Patent ID: 7588888

Claim:
A method for identifying a polynucleotide encoding a deoxyhypusine synthase (DHS) in a plant comprising: a) exposing a tissue of said plant to injury; b) isolating RNA from the injured tissue; c) constructing a cDNA library from the RNA obtained from the injured tissue; d) screening the library with a known plant DHS polynucleotide to identify a cDNA in the library that hybridizes to the known DHS polynucleotide; e) sequencing said identified cDNA and aligning said sequence against the sequence of the DHS polynucleotide; and f) identifying a DHS polynucleotide having at least 73% sequence identity across the entire coding region of the DHS with that of one of said known plant DHS polynucleotide to identify a polynucleotide encoding a DHS in said plant.