Patent ID: 8334426

Claim:
A method for producing a transformed maize plant, said method comprising: (a) isolating an immature embryo from a maize ear; (b) introducing a polynucleotide of interest into at least one cell of said immature embryo to produce a transformed maize cell; (c) culturing said immature embryo having a transformed cell on a callus selection media comprising an effective amount of an auxin for a time period and conditions sufficient for early and late callus formation events to occur; (d) identifying at least one early or late event from the culture of step (c); (e) identifying a micro event from the culture of step (c) wherein the micro event is a viable non-proliferating group of cells from a single transgenic cell compared to early and late events in steps (c) to (d) and the micro event having a somatic immature embryo morphology; and (f) regenerating a transformed maize plant from the micro event of step (e). wherein the transformed maize plant comprises the polynucleotide of interest.