Patent ID: 8415155

Claim:
A method for producing human cardiomyocytes comprising the steps of: a) obtaining a selected human pluripotent stem cell clone having been prepared from a single pluripotent stem cell, the human stem cell clone being either a human embryonic stem cell clone or a human induced pluripotent stem cell clone; b) culturing human pluripotent stem cells from the selected cell clone in a selected culture media batch, wherein the selected culture media is a maintenance culture media that maintains the human pluripotent stem cells in an undifferentiated state; c) determining the level of one or more differentiation factors appropriate for cardiac differentiation of the selected human pluripotent stem cell clone cultured in the selected culture media batch; d) obtaining a cardiac differentiation medium prepared by adjusting the level of the one or more differentiation factors in the cardiac differentiation medium according to the results obtained in step c); and e) differentiating the human pluripotent stem cells cultured in step b) into cardiomyocytes by culturing the human pluripotent stem cells in the cardiac differentiation medium obtained in step d).