Patent ID: 7037719

Claim:
A method for producing a population highly enriched for human central nervous system stem cells (CNS-SC) which can initiate neurospheres (NS-IC), comprising: selecting from a population containing neural or neural-derived cells for cells that bind to a first antibody selected from the group consisting of monoclonal antibody AC133 and monoclonal antibody 5E12, and further comprising the step of further enriching the population by selecting and eliminating from the population those cells that bind to a second antibody selected from the group consisting of a monoclonal antibody that binds to CD45 antigen and a monoclonal antibody that binds to CD34 antigen, such that those cells that are AC133 + CD45 − or AC133 + CD34 − or 5E12 + CD45 − or 5E12 + CD34 − are selected.