Patent ID: 7009700

Claim:
A method of obtaining three-dimensional images of a specimen and of a field of fluorescence emitted by the specimen, the method comprising, with a system able to operate in digital holography microscopy: a) obtaining simultaneously in one step all the digital holographic information of the entire volume of the specimen; b) obtaining simultaneously in one step all the fluorescence signals emanating from the entire volume of the specimen; c) recording all the digital holographic information and all the fluorescence signals emanating from the entire volume of the specimen; d) processing all the digital holographic information so as to reconstruct a three-dimensional image of the entire volume of the specimen at a given time; and e) processing all the fluorescence signals so as to reconstruct a three-dimensional image of the field of fluorescence emitted by the entire volume of the specimen at a given time, said processing including combining all the fluorescence signals with all the digital holographic information.