Patent ID: 8841248

Claim:
A method for preparing an enriched Inter-alpha-Inhibitor protein (IaIp) composition from plasma, the method comprising the steps of: (i) forming a Fraction II+III precipitate from a plasma sample by: (a) precipitating proteins from a Cohn pool, in a first precipitation step, with between about 6% and about 10% alcohol at a pH of between about 7.0 and about 7.5 to obtain a first precipitate and a first supernatant, and (b) precipitating proteins from the first supernatant, in a second precipitation step, with between about 20% and about 25% alcohol at a pH of between about 6.6 and about 7.2, thereby forming a Fraction II+III precipitate; (ii) suspending the Fraction II+III precipitate to form a Fraction II+III suspension; (iii) contacting the Fraction II+III suspension with a solid phase to remove the IaIp from the Fraction II+III suspension; and (iv) extracting the IaIp from the solid phase, thereby preparing an enriched IaIp composition.