Patent ID: 8445196

Claim:
A random array of concatemers, wherein said random array comprises: (a) a solid substrate comprising a surface, wherein said surface comprises a plurality of discrete spaced apart regions defined on said surface, wherein said discrete spaced apart regions are separated by inter-regional areas; (b) concatemers randomly disposed on said plurality of discrete spaced apart regions such that at least a majority of said plurality of discrete spaced apart regions comprises a single concatemer immobilized thereon and said concatemers do not bind to said inter-regional areas, wherein said concatemers comprise a plurality of monomeric units and each monomeric unit comprises: (i) a first target sequence of a target polynucleotide of unknown sequence; (ii) an adaptor comprising a Type Ils endonuclease restriction site, wherein said first target sequence is adjacent to said adaptor; and wherein each said single concatemer is immobilized on a discrete spaced apart region by attractive noncovalent interactions between said discrete spaced apart region and multiple monomeric units of said single concatemer.