Patent ID: 7101673

Claim:
A method for detecting a nucleic acid, comprising the steps of: carrying out amplification reaction to amplify a predetermined region of the nucleic acid in a reaction solution comprising the nucleic acid acting as a template, a pair of primers, deoxynucleotides, and dideoxynucleotides; hybridizing amplified products of the reaction solution to a probe; and detecting the hybridization between the amplified products and the probe; and wherein the amplification reaction is carried out using a reaction solution in which the concentration ratio of the deoxynucleotides to the dideoxynucleotides meets the equation; 0.1 <{dNTP concentration/( dNTP concentration+ ddNTP concentration)} ab ≧1.0 wherein “dNTP concentration” represents the concentration of deoxynucleotides in the reaction solution, “ddNTP concentration” represents the concentration of dideoxynucleotides in the reaction solution, “a” represents the number of nucleotides extended in the amplification reaction (1≦a), and “b” represents the cycle number of the amplification reaction.