Patent ID: 8436153

Claim:
A cyanine dye of the formula: wherein R 1 is a member selected from substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl groups and does not comprise a carboxylic acid ester moiety; R 2 is a member selected from substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl with the proviso that R 2 comprises a group that is a member selected from oxygen-containing reactive functional groups, solid supports and carrier molecules wherein said oxygen-containing reactive functional group is a member selected from hydroxyl and phosphoramidite; R 3 is a member selected from H, substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl, or R 2 and R 3 , together with the nitrogen to which they are attached, are joined to form a ring which is substituted 5-7-membered heterocycloalkyl; each of L is C(R 17 ) wherein each R 17 is a member independently selected from H, halide, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl, and wherein two adjacent R 17 groups may join to form a ring; n is an integer from 1 to 4; R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , and R 11 are members independently selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heterocycloalkyl, halogen, H, NO 2 , CN, Z 1 R 19 , NR 20 R 21 , and C(Z 2 )R 22 ; wherein Z 1 is a member selected from O and S; Z 2 is a member selected from O, S and NH; R 19 and R 20 are members independently selected from H, substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl; R 21 is a member selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl and C(Z 3 )R 22 wherein Z 3 is a member selected from O, S and NH; R 22 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl and OR 23 , and NR 24 R 25 , wherein R 23 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and C(O)R 26 wherein R 26 is a member selected from substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl; R 24 and R 25 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl; or R 20 and R 21 , together with the nitrogen to which they are attached, are members selected from —NHNH 2 , —N═C═S and —N═C═O; R 12 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl; and R 13 , R 14 , R 15 and R 16 are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl.