Patent ID: 7595152

Claim:
A method of detecting influenza A, comprising: (a) contacting a sample from a subject with a first agent comprising a PDZ domain that specifically binds to a PDZ-binding ligand sequence (PL) of an NS1 protein of a pathogenic subtype of influenza A; (b) detecting presence or absence of specific binding of the first agent to any NS1 protein of a pathogenic influenza A in the sample wherein presence of specific binding to an NS1 protein indicates presence of the pathogenic influenza A subtype in the sample, and absence of specific binding to any NS1 protein indicates absence of the pathogenic influenza A subtype in the sample; (c) contacting the patient sample with a second agent comprising a PDZ domain that specifically binds to a PL of an NS1 protein of a seasonal subtype of influenza A; and (d) detecting presence or absence of specific binding of the second agent to any NS1 protein of the seasonal subtype of influenza A in the sample wherein presence of specific binding to an NS1 protein indicates presence of the seasonal subtype of influenza A in the sample, and absence of specific binding to any NS1 protein indicates absence of the seasonal subtype of influenza A in the sample.