Patent ID: 8368035

Claim:
A method for 3D imaging of a biological object in an optical tomography system comprising: moving a biological object relative to a microscope objective to present varying angles of view; illuminating the entire biological object; axially scanning the entire biological object with the microscope objective at a fixed viewpoint through the thickness of the biological object where an axial scan is performed at each of said varying angles of view; sensing a first radiation bandwidth transmitted through the entire biological object and the microscope objective with an ultraviolet camera during axially scanning, wherein the first radiation bandwidth has a first spectral range between 150 nm and 390 nm to produce a first plurality of pseudo-projection images of the specimen onto the ultraviolet camera; sensing a second radiation bandwidth within a second spectral range which is also transmitted through the entire biological object and the microscope objective to the ultraviolet camera during axially scanning, wherein the second spectral range is also between 150 nm and 390 nm, but different than the first spectral range, to produce a second plurality of pseudo-projection images of the specimen onto the ultraviolet camera; wherein each axial scan is performed during a single exposure of the ultraviolet camera, and wherein each pseudo-projection image thus formed includes an integration of a range of focal plane images; and reconstructing the plurality of pseudoprojection images to form a reconstructed 3D image.