Patent ID: 7501237

Claim:
A method of identifying, analyzing or typing a polymorphic DNA fragment in a sample of DNA, said method comprising: (a) contacting said sample of DNA with one or more DNA polymerases, wherein said one or more DNA polymerases comprise one or more mutations or modifications in the O-helix of said one or more DNA polymerases which reduce their ability to add one or more non-templated nucleotides to the 3′ terminus of a synthesized DNA molecule; (b) amplifying said polymorphic DNA fragment within said sample to produce a population of amplified DNA fragments, wherein less than about 50% of said amplified DNA fragments have one or more non-templated 3′ nucleotides compared to amplification products produced by Taq DNA polymerase assayed under the same conditions; and (c) analyzing said amplified polymorphic DNA fragment.