Patent ID: 6902903

Claim:
A method of detecting Helicobacter pylori antibodies associated with infection in a human subject comprising: (a) reacting a biological sample from the subject with one or more H. pylori type-common antigens provided in an H. pylori lysate and with one or more purified type-specific H. pylori Type I antigens, wherein the type-specific antigens are H. pylori vacuolating cytotoxin (VacA) and cytotoxin associated antigen (CagA), under conditions which allow H. pylori antibodies, when present in the biological sample, to specifically bind with said type-common antigens or said type-specific antigen(s); (b) removing unbound antibodies; (c) providing one or more moieties comprising a detectably labeled anti-human immunoglobulin antibody which bind to said bound antibodies; (d) detecting the presence or absence of said one or more moieties; (e) correlating the presence of antibodies that specifically bind to the type-specific antigens to infection with Type I H. pylori ; and (f) correlating the absence of antibodies that specifically bind to the type-specific antigens and the presence of antibodies that specifically bind to the type-common antigens to infection with Type II H. pylori.