Patent ID: 7238518

Claim:
An oligonucleotide-immobilized substrate for detecting presence or absence of methylation of C in a sample DNA comprising a target sequence comprising a plurality of CpG dinucleotides, consisting of capture probes, said capture probes consisting of multiple pairs of capture oligonucleotides wherein the capture oligonucleotide pairs consist of (a) an oligonucleotide consisting of a nucleotide sequence complementary to or identical to a nucleotide sequence corresponding to the target sequence in which all of C's other than C's in all CpG dinucleotides (C that can be methylated and G on the 3′ side of the C) are replaced with T's and comprising a plurality of CpG dinucleotides and (b) an oligonucleotide consisting of a nucleotide sequence complementary to or identical to a nucleotide sequence corresponding to the target sequence in which all of C's are replaced with T's immobilized thereon and comprising a plurality of CpG dinucleotides from said target sequence.