Patent ID: 7501268

Claim:
A method of producing a geranylgeraniol in a yeast host, wherein the concentration of said geranylgeraniol accumulated in a culture is at least 0.1 g/L, said method comprising creating a recombinant by transferring into the host: (a) a recombinant DNA for expression or a DNA for genomic integration each comprising a fusion gene comprising in the following order (i) a geranylgeranyl diphosphate (GGPP) synthase gene encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:6 or a polypeptide having GGPP synthase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:6 by deletion, substitution, or addition of from one to ten amino acids, and (ii) a farnesyl diphosphate (FPP) synthase gene encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:2 or 4 or a polypeptide having FPP synthase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:2 or 4 by deletion, substitution, or addition of from one to ten amino acids, and (b) a recombinant DNA for expression or a DNA for genomic integration each comprising a hydroxymethylglutaryl-CoA (HMG-CoA) reductase gene encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:10 or a polypeptide having HMG-CoA reductase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:10 by deletion, substitution, or addition of from one to ten amino acids, culturing the resultant recombinant, and recovering the geranylgeraniol from the resultant culture.