Patent ID: 8912143

Claim:
A synthesized polypeptide optimized for a Pichia pastoris expression system, wherein said polypeptide is capable of anticoagulation, comprising a synthesized Long-half-life Tissue Factor Pathway Inhibitor (LTFPI) polypeptide represented by SEQ ID NO: 2 which is mutated from human wild-type Tissue Factor Pathway Inhibitor (TFPI), wherein the lysines of the polypeptide at the carboxy-terminal residues 241, 254, 260 and 261, the asparagines at glycosylation residues 117, 167, 228, and the serine and threonine at glycosylation residues 174 and 175 are all substitutionally mutated to alanine, and wherein the polypeptide is optimized for Pichia pastoris expression.