Patent ID: 7521058

Claim:
A non-spreading pestivirus vaccine produced by a process comprising: obtaining a multitude of pestivirus-like particles produced by a process comprising: providing a cell permissive for pestiviral infection; introducing a first recombinant nucleic acid sequence comprising a pestiviral genomic sequence having at least one functional deletion, wherein the functional deletion is selected from the group consisting of a deletion of amino acids 381 to 422, 405 to 436, 273 to 488, 422 to 488, 436 to 488, 422 to 436, and combinations thereof in E rns corresponding to amino acids 118 to 161, 144 to 175, 6 to 229, 161 to 229, 175 to 229, 161 to 175, respectively, of SEQ ID NO: 22, and said first recombinant nucleic acid sequence encoding at least one structural protein or an immunodominant portion of the structural protein; introducing into said cell a nucleic acid construct, wherein a second nucleic acid sequence therein complements said at least one functional deletion in said first recombinant nucleic acid sequence; and replicating said first recombinant nucleic acid sequence in said cell, thus producing a pestivirus-like particle having the first replicated recombinant nucleic acid sequence; and preparing a suspension of said multitude of pestivirus-like particles in a suitable diluent.