Patent ID: 8221765

Claim:
A polypeptide comprising: (a) an N-terminal domain comprising a first fibronectin type III tenth domain ( 10 Fn3), wherein the 10 Fn3 domain (i) comprises a loop, AB; a loop, BC; a loop, CD; a loop, DE; a loop EF; and a loop FG; (ii) has at least one loop selected from loop BC, DE, and FG with an altered amino acid sequence relative to the sequence of the corresponding loop of the human 10 Fn3 domain, (iii) comprises an amino acid sequence that is at least 60% identical to SEQ ID NO: 1, and (iv) binds to a first target molecule with a K D of less than 500 nM; and (b) a C-terminal domain comprising a second fibronectin type Ill tenth domain ( 10 Fn3), wherein the 10 Fn3 domain (i) comprises a loop, AB; a loop, BC; a loop, CD; a loop, DE; a loop EF; and a loop FG; (ii) has at least one loop selected from loop BC, DE, and FG with an altered amino acid sequence relative to the sequence of the corresponding loop of the human 10 Fn3 domain, (iii) comprises an amino acid sequence that is at least 60% identical to the amino acid sequence of SEQ ID NO: 1, and (iv) binds to a second target molecule with a K D of less than 500 nM; wherein the polypeptide comprises the amino acid sequence of any one of SEQ ID NOs: 8-15, 29-31, and 63-64.