Patent ID: 7037693

Claim:
A method of preparing 3-(Z)-nonenal, 2-(E)-nonenal, or their corresponding alcohols from (9S,10E,12Z) 9-hydroperoxyoctadeca-10,12-dienoic acid, comprising (a) contacting the (9S,10E,12Z) 9-hydroperoxyoctadeca-10,12-dienoic acid with an isolated fatty acid hydroperoxide lyase having activity for both 9-hydroperoxide substrates and 13-hydroperoxide substrates, comprising the amino acid sequence SEQ ID NO:7, wherein the lyase has a 9-HPL function, thereby converting the (9S,10E,12Z) 9-hydroperoxyoctadeca-10,12-dienoic acid into 3-(Z)-nonenal; and either (b) recovering the 3-(Z)-nonenal; (c) reducing the 3-(Z)-nonenal into 3-(Z)-nonenol and recovering the 3-(Z)-nonenol; or (d) isomerizing the 3-(Z)-nonenal under temperature and pH conditions effective to obtain 2-(E)-nonenal and either recovering the formed 2-(E)-nonenal or reducing the 2-(E)-nonenal to 2-(E)-nonenol and recovering the 2-(E)-nonenol from the medium.