Patent ID: 8680320

Claim:
A kit for detecting metal ions in a sample, wherein the kit comprises a compound having the formula: wherein R X is —CH 2 CO 2 R; R Y is —CH 2 CO 2 R; R X ′ is —CH 2 CO 2 R; wherein R is H, a salt ion or —CH 2 OC(O)(CH 2 ) n CH 3 and n is 0 to 6; R 1 is hydrogen; R 2 is hydrogen; R 3 is hydrogen, halogen, alkyl, substituted alkyl, alkoxy, substituted alkoxy, hydroxyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, amino, substituted amino, alkylamino, substituted alkylamino, aldehyde, carboxyl, azido, nitro, nitroso, cyano, thioether, sulfoalkyl, carboxyalkyl, aminoalkyl, reporter molecule, reactive group, carrier molecule or solid support; R 4 is hydrogen; R 5 is hydrogen; R 6 is hydrogen, halogen, alkyl, substituted alkyl, alkoxy, substituted alkoxy, hydroxyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, amino, substituted amino, alkylamino, substituted alkylamino, aldehyde, carboxyl, azido, nitro, nitroso, cyano, thioether, sulfoalkyl, carboxyalkyl, aminoalkyl, reporter molecule, reactive group, carrier molecule or solid support; R 7 is hydrogen, halogen, alkyl, substituted alkyl, alkoxy, substituted alkoxy, hydroxyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, amino, substituted amino, alkylamino, substituted alkylamino, aldehyde, carboxyl, azido, nitro, nitroso, cyano, thioether, sulfoalkyl, carboxyalkyl, aminoalkyl, reporter molecule, reactive group, carrier molecule or solid support; R 8 is hydrogen; R 9 is hydrogen; R 10 is hydrogen; R 11 is hydrogen; and R 12 is hydrogen.