Patent ID: 7262291

Claim:
A polynucleotide molecule encoding a modified Clostridial toxin comprising: a) a first polynucleotide region encoding a fusion protein of an orphanin polypeptide and a translocation domain comprising: i) a first polynucleotide segment encoding an orphanin polypeptide as a targeting moiety that binds to an orphanin receptor; and ii) a second polynucleotide segment encoding a Clostridial toxin H N domain or fragment thereof that translocates a Clostridial toxin light chain or light chain fragment thereof across an endosome membrane; and b) a second polynucleotide region encoding a Clostridial toxin light chain or light chain fragment thereof that cleaves a neurosecretory protein, wherein the H C domain of the modified Clostridial toxin is removed or modified in order to reduce the binding of the modified Clostridial toxin to Clostridial toxin receptors at the neuromuscular junction.