Patent ID: 6951716

Claim:
A process for producing anti-platelet Ig control coated beads for flow cytometric assays, comprising the steps of: labeling three test-tubes IgG, IgM and IgA; placing a carbonate buffer into each of the three test-tubes; placing an amount of micron beads into each of the three test-tubes, the micron beads in each test-tube having a predetermined size; gently vortexing the resulting solution; centrifuging the solution to substantially separate the beads from the buffer solution; removing the supernatant buffer solution from the beads; vortexing the beads to create a uniform suspension; adding an amount of carbonate buffer to the beads sufficient to achieve resuspension of the beads; adding one or immunoglobulins IgG, IgM and IgA to each of the three test-tubes labeled IgG, IgM and IgA respectively; vortexing the resulting solution; incubating the solution until attachment of the immunoglobulin to the beads has occurred; resuspending the buffer/bead mixture; centrifuging the buffer/bead mixture to separate out unattached immunoglobulin; decanting supernatant immunoglobulin-coated beads and gently resuspending; and adding an amount of carbonate butter to stabilize the coated beads.