Patent ID: 8329448

Claim:
A mutant Schizosaccharomyces pombe cell that has a deletion or inactivation of at least three endogenous target genes whereby the level of expression of a gene encoding a heterologous protein in said mutant Schizosaccharomyces pombe host cell is higher compared to a corresponding Schizosaccharomyces pombe cell that does not have a deletion or inactivation of the at least three endogenous target genes; (i) wherein at least one of the deleted or inactivated target genes is selected from the group consisting of cdb4 (SPAC23H4.09), and ppp53 (SPAP 14E8.04); and (ii) wherein at least two of the deleted or inactivated target genes are selected from the group consisting of isp6 (SPAC4A8.04), ppp16 (SPBC 1711.12), psp3 (SPAC 1006.01) and sxa2 (SPAC 1296.03c).