Patent ID: 7402429

Claim:
An isolated eukaryotic host cell comprising (1) stably transfected expression plasmids that allow genomic expression of an exogenous RNA polymerase, a viral N protein, and a viral P protein in the host cell; (2) a nucleic acid sequence encoding a viral L protein; and (3) a cDNA molecule, said cDNA molecule comprising: (a) a (+) strand sequence of a non-segmented negative-strand RNA virus of the family Paramyxoviridae from which RNA transcripts bearing the authentic 3′ termini can be transcribed; and (b) an expression control sequence, wherein said (+) strand sequence of a non-segmented negative-strand RNA virus is operatively linked to the expression control sequence, and wherein the host cell, without a replicating vaccinia helper virus, produces an infectious RNA virus containing the (−) strand sequence of the non-segmented negative-strand RNA virus of the family Paramyxoviridae, said (−) strand sequence of the non-segmented negative-strand RNA virus obeying the rule of six.