Patent ID: 6905835

Claim:
A method for detecting IgA or IgG anti-transglutaminase antibodies in liquid samples, the method comprising: a) providing a system comprising: (i) an inert porous support wherein tissue transglutaminase antigen conjugated to a colored substance is deposited and dried, and wherein the support allows release and laminar flow of the conjugated antigen when contacted with a liquid sample; and (ii) a membrane comprising a reactive zone which comprises immobilized tissue transglutaxninase antigen; b) obtaining a liquid sample from a human; c) adding the liquid sample to the inert porous support containing tissue transglutaminase antigen conjugated to a colored substance, wherein the conjugated antigen and/or immunocomplexes formed between antibodies in the sample and the conjugated antigen migrate to the reactive zone by laminar flow; and d) detecting any binding between the immunocomplexes formed in step c) with the immobilized antigen, wherein binding of the immunocomplexes with the immobilized antigen in the reactive zone indicates the presence of IgA or IgG anti-transglutaminase antibodies in the sample.