Patent ID: 8709761

Claim:
A method of reducing cost of producing glucose from cellulosic biomass, the method comprising, (a) providing cellulosic biomass comprising cellulose; (b) providing an exogenous cellulose degrading composition at a first amount, said composition comprising a first amount of each of endo-β-1,4-glucanase, exo-β-1,4-glucanase, and β-D glucosidase, said composition capable of converting a first amount of cellulose to a first amount of glucose within 72 hours after contact of said cellulose with said cellulose degrading composition; (c) providing a plant embryo tissue composition wherein any endogenous enzymes in said plant embryo tissue are capable of converting said first amount of cellulose to a second amount of fermentable sugar within 72 hours after contacting said cellulose with said plant tissue composition; (d) contacting said plant embryo tissue compositions, said cellulose degrading enzyme composition and said cellulosic biomass; (e) reducing cost by a process elected from (i) providing said cellulosic biomass wherein said cellulosic biomass comprises plant embryo tissue, and not removing or reducing any lignin in said cellulosic biomass prior to contact with said cellulose degrading composition; (ii) providing said cellulosic biomass is plant embryo tissue and not adding further cellulosic biomass; (iii) excluding from said cellulose degrading composition β-D glucosidase and producing glucose; (iv) excluding from said cellulose degrading composition β-D glucosidase and/or one of endo-β-1,4-glucanase or exo-β-1,4-glucanase, and producing at least the same amount of glucose as said first amount of glucose; (v) providing said exogenous cellulose degrading composition or at least one of said endo-β-1,4-glucanase, exo-β-1,4-glucanase or β-D glucosidase at a lower amount compared to said first amount and producing at least at least the same amount of glucose as said first amount of glucose; (vi) producing glucose within 72 hours after contact of said cellulose with said cellulosic biomass, cellulose degrading composition and said plant tissue composition at a third amount that is greater than the total of said first amount of glucose and said second amount of glucose; and (f) producing glucose, wherein cost of producing said glucose is reduced.