Patent ID: 8236555

Claim:
A method for measuring analytes in a sample, the method comprising: a) contacting a first dilution of said sample with one or more binding surfaces having binding reagents that bind a first analyte and a second analyte, wherein said one or more binding surfaces are located within a measurement chamber; b) contacting said binding surface(s) with a first detection reagent, wherein said first detection reagent binds to said first analyte; c) contacting a second dilution of said sample with said binding surface(s) in said measurement chamber, said second dilution having a dilution factor less than said first dilution; d) contacting said binding surface(s) with a second detection reagent, wherein said second detection reagent binds to said second analyte; and e) measuring the amount of said first detection reagent and said second detection reagent bound to said binding surface(s).