Patent ID: 8303896

Claim:
A method for immobilizing a physiologically active substance, which comprises: preconcentrating a physiologically active substance having a carboxyl group at a pH that is equivalent to or higher than the isoelectric point of the physiologically active substance, on a solid substrate to which a polymer having a primary or secondary amino group is bound; and causing a carboxylic acid activator to come into contact with the substrate, so as to bind the polymer having the amino group to the physiologically active substance, wherein the polymer having the primary or secondary amino group is a polymer obtained by causing a polymer having a carboxyl group to react with a polyamine, wherein the polyamine is at least one selected from the group consisting of 1,2-bis(2-aminoethoxy)ethane, ethylenediamine, tetraethylenediamine, octamethylene diamine, decamethylene diamine, piperazine, triethylenediamine, diethylenetriamine, triethylenetetraamine, dihexamethylene triamine, 1,4-diamino cyclohexane, paraphenylenediamine, methaphenylene diamine, paraxylylenediamine, metaxylylenediamine, 4,4′-diaminobiphenyl, 4,4′-diamino diphenylmethane, 4,4′-diamino diphenylketone, 4,4′-diaminodiphenyl sulfonic acid, diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentaethylenehexamine, spermidine, spermin, and polyethyleneimine.