Patent ID: 8394633

Claim:
A method of dispersion of clusters of plant embryos suspended in a liquid into individual plant embryos flowing through a flow channel, the method comprising: feeding clusters of plant embryos into a disperser for separating individual somatic plant embryos contained in the clusters of embryos, subjecting the clusters of plant embryos to axially extensional strain and radially compressional strain; subjecting the clusters of plant embryos to fluid dynamics forces causing axially compressional strain and radially extensional strain from fluid dynamics forces; and repeating the subjecting steps in sequence until the individual plant embryos are separated from each other, wherein the disperser comprises a flow channel including at least two constrictions, wherein each constriction of the at least two constrictions has an inner diameter that is equal to or smaller than the inner diameter of the constriction immediately up-stream thereof, wherein each constriction of the at least two constrictions has a cross-sectional area in the interval from 0.75 to 1300 mm 2 wherein the minimum cross-sectional dimension of the flow channel is larger than the largest dimension of the largest embryo in the clusters of plant embryos to be dispersed, wherein the flow channel includes an intermediate portion between sequential constrictions, the intermediate portion having an essentially constant cross-section.