Patent ID: 7229777

Claim:
A method of identifying peptides capable of binding to a bioactive target molecule comprising a) providing a library of peptides comprising a trpzip scaffold, wherein each peptide comprises a presented turn sequence and a trpzip scaffold comprising a first and a second opposite strand with a defined backbone hydrogen-bonding pattern, each strand comprising a trpzip domain of at least two Trp residues at non-hydrogen-bonded positions, and each trpzip domain consists of the amino acid sequence WX 1 W, wherein X 1 is independently Thr or independently an amino acid selected from the group consisting of H, V, I, F, Y, and W, wherein the Trp residues from each trpzip domain form a cross-strand pair without any disulfide bond, wherein the presented turn sequence is flanked by the first and second opposite strands and comprises random amino acids; b) contacting the library with the bioactive target molecule; c) selecting at least one peptide capable of forming a noncovalent complex with the bioactive target molecule from the library; and d) optionally, isolating the at least one selected peptide.