Patent ID: 8058053

Claim:
A genetically engineered Pichia strain which produces glycoproteins comprising a core N-glycan structure characterized by the formula, GalGlcNAcMan5 or fewer GlcNAc2, wherein said strain expresses (1) an Î±-1,2-mannosidase or an enzymatically active fragment thereof which converts Man8GlcNAc2 to Man5GlcNAc2, (2) an N-acetylglucosaminyltransferase I (or GnTI) or an enzymatically active fragment thereof which converts Man5GlcNAc2 to GlcNAcMan5GlcNAc2, and (3) a Î²-1,4-galactosyltransferase (GalT) or an enzymatically active fragment thereof, wherein the genomic OCH1 gene of said strain is inactivated, and wherein at least about 10% of GlcNAcMan5GlcNAc2 is converted to GalGlcNAcMan5GlcNAc2.