Patent ID: 8137948

Claim:
A method for purifying AAV vector particles from an AAV preparation comprising AAV vector particles and AAV empty capsids, to provide an AAV product substantially free of AAV empty capsids, said method comprising: (a) applying an AAV preparation comprising AAV vector particles and AAV empty capsids to an anion exchange chromatography column under conditions whereby said AAV vector particles and said AAV empty capsids bind the column; (b) adding a salt buffer to the column from (a) under conditions whereby AAV empty capsids are eluted and AAV vector particles remain bound to the column; (c) adding a salt buffer with a greater salt concentration relative to the buffer added in (b) to the column from (b) under conditions whereby AAV vector particles are eluted; (d) collecting eluted fractions from (c) and selecting fractions that consist essentially of AAV vector particles based on the ratio of UV absorption at 260 nm/280 nm to provide an AAV product substantially free of AAV empty capsids.