Patent ID: 8431365

Claim:
A method for transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage from a N-acetyl-D-galactosamine donor substrate to an acceptor substrate containing N-acetyl-D-glucosamine, which comprises reacting the N-acetyl-D-galactosamine donor substrate, the acceptor substrate containing N-acetyl-D-glucosamine, and a polypeptide selected from the group consisting of the following: i) a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 4; ii) a polypeptide having the amino acid sequence covering amino acids 35 to 504 shown in SEQ ID NO: 4; iii) a polypeptide encoded by a nucleic acid sequence that hybridizes with the nucleic acid sequence shown in SEQ ID NO: 3 under high stringency conditions of hybridization at 50° C., 2×SSC, and 50% formamide and washing at 68° C., 0.2×SSC, and 0.1% SDS, which transfers N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage.