Patent ID: 6893849

Claim:
A submerged fermentation process for the production of xylitol using yeast strains which comprises the following steps: a) obtaining an inoculum of yeast strain Pichia sp.; b) inoculating the inoculum in a 10 ml sterile medium containing the ingredients (% w/v) glycerol within a range of 2.0-5.0, xylose within a range of 4.0-6.0, yeast extract within a range of 0.1-1.0, peptone within a range of 0.5-2.0, ammonium chloride within a range of 0.1-1.0, dipotassium hydrogen phosphate within a range of 0.1-0.5, potassium dihydrogen orthophosphate within a range of 0.1-0.5, ferrous sulphate within a range of 0.001-0.01 and magnesium sulphate within a range of 0.01-0.1 at a final pH in the range of 5.5 to 6.5; c) growing the inoculum for 18-24 hours at 30±1° C. in a shaker at 200-300 rpm, and d) fermenting the sterile medium containing the following ingredients (in % w/v) glycerol within a range of 2.0-5.0, xylose within a range of 2.0-5.0, cotton seed flour within a range of 1.0-5.0, ammonium chloride within a range of 0.1-1.0, dipotassium hydrogen phosphate within a range of 0.1-0.5, potassium dihydrogen orthophosphate within a range of 0.1-0.5, ferrous sulphate within a range of 0.001-0.01 and magnesium sulphate within a range of 0.01-0.1 using the inoculum of step (c) under stirring at 30±1° C. for 24-90 hours and obtaining the xylitol.