Patent ID: 8541242

Claim:
A device for detecting at least one substance of interest in a liquid sample, the device comprising: (a) a receptacle comprising a porous material for receiving the liquid sample, wherein the porous material is capable of absorbing and transmitting at least a portion of the liquid sample, and (b) a porous membrane that comprises a specific binding pair member that is specific for the substance of interest or a substance bound to the substance of interest, wherein the receptacle and porous membrane are each shaped to permit the porous membrane to be in direct contact with the porous material over at least a portion of the porous membrane that comprises the specific binding pair member such that the porous material is in physical contact with a bottom side of the porous membrane, and wherein the specific binding pair member is in continuous contact with the porous material to allow for continuous diffusion of the liquid sample between the porous material and the specific binding pair member, which is located directly above the porous material to which the liquid sample was applied.