Patent ID: 8450098

Claim:
A method for introducing one or more nucleic acids into filamentous fungal spores to produce transformed fungal spores, comprising mixing one or more filamentous fungal spores with one or more nucleic acids; and applying an electric field under conditions that allow introduction of the one or more nucleic acids into one or more fungal spores, wherein the electric field is applied at a voltage of between about 15,500 V/cm and about 18,500 V/cm to produce filamentous fungal spores, wherein the electric field is applied with a device having a resistance of between 45Ω and 65Ω, wherein the electric field is applied at a capacitance of between 20 μF and 100 μF, wherein the number of transformants in a population is between about 400 transformants/μg DNA and about 5 transformants/μg DNA.