Patent ID: 8062876

Claim:
A method for converting prethrombin-1 to thrombin comprising: providing prethrombin-1 at a concentration of 0.1 mg/mL to 10 mg/mL in an aqueous solution of 30 mM to 110 mM NaCl at pH 6.4-8.0; providing oscutarin-C immobilized on a solid support; and applying the aqueous solution to the immobilized oscutarin-C so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes inclusive, whereby the prethrombin-1 is cleaved to produce thrombin, and a thrombin-containing solution is obtained.