Patent ID: 7288371

Claim:
A method for detecting an isolate of Bacillus anthracis in a sample, the method comprising: (a) placing on a microchip a set of oligonucleotide probes targeted to rRNA sequences, wherein the set of oligonucleotide probes comprises the probe sequences as set forth in (i) TTT GGG CTA TGT TCC GTT TC (SEQ ID NO: 57) (ii) TTT GGG CTA GAT TCC GTT TC (SEQ ID NO: 58) (iii) CAG CTC AGC CTT CAC GAT AA (SEQ ID NO: 97) (iv) CAG CTC AGC CTT TAC GAT AA (SEQ ID NO: 98) (v) TCT AGG GTT GTC AGA GGA TG (SEQ ID NO: 76) (vi) TCT AGG GTT TTC AGA GGA TG (SEQ ID NO: 75) (vii) CCG CTA ACT TCA TAA GAG CA (SEQ ID NO: 103) (viii) CCG CTA ACT TCT TGA GAG CA (SEQ ID NO: 104) (ix) reverse complement of GCTTCTCCTTCGGGAGCAGA (SEQ ID NO: 19); (x) reverse complement of GCTTCcCCTTCGGGgGCAGA (SEQ ID NO: 20); and (xi) CCC TCT GTG ACG AGC CTT TC (SEQ ID NO: 72) and discriminates among the B. cereus subgroups; (b) providing conditions for hybridization of the probes with nucleic acids from the sample; and (c) analyzing hybridization signals in the microchip to detect Bacillus anthracis.