Patent ID: 7560097

Claim:
A method of manufacturing a preparation containing magnetic vesicular particles, the method comprising: (a) mixing at least one lipid membrane constituent and a supercritical carbon dioxide to form a mixture, (b) adding to the mixture a dispersion containing magnetic nanoparticles to which an organic compound is bonded, and (c) discharging the carbon dioxide to form the magnetic vesicular particles including the magnetic nanoparticles within a lipid membrane, wherein the magnetic vesicular particles, each includes at least one magnetic nanoparticle within a lipid membrane, an organic compound having at least two groups selected from the group consisting of a hydroxyl group, a carboxyl group, a carbamoyl group, an amino group, a mercapto group, a sulfo group, a dithio group, a thiocarboxyl group and a dithiocarboxyl group is bonded to the magnetic nanoparticle, and the magnetic vesicular particles satisfy the following equation: 0.05 ≦R /( r× 100)≦1.5 wherein R represents an average grain size of the magnetic vesicular particles and r represents an average particle size of magnetic nanoparticles included in the magnetic vesicular particles wherein the organic compound is a compound selected from the group consisting of phthalic acid, isophthalic acid, therephthalic acid, succinic acid, glutaric acid, adipic acid, pimelic acid, fumaric acid, maleic acid, 2-mercaptoamine, 6-aminehexanethiol, 2-mercaptopropionic acid, asparagic acid, glutamine, malic acid, oxaloacetic acid, 2-ketoglutaric acid, serine, threonine, cysteine, cysteic acid, cystine, N-acetylcysteine, cysteine ethyl ester, and dithiothreitol.