Patent ID: 7662582

Claim:
A method of identifying progress of cancer comprising: obtaining a 50 ml urine sample from a person who has fasted for at least six hours: removing insoluble materials and red blood cells from the urine by centrifuging the urine at 3000 rpm for 15 minutes at 4° C., and producing first supernatants; centrifuging the first supernatants at 15,000 g for 30 minutes at 4° C. to produce second supernatants; loading the second supernatants onto a membrane in a centrifuging filter device; producing concentrated proteins and removing interference molecules by operating the centrifuging filter device to spin at 3700 g at 4° C. for 1 hour by which concentrated urine is produced by the operation reducing volume of the second supernatants to 1 ml; adding two volumes of cold 20% TCA to the concentrated urine to produce an intermediate mixture; storing the intermediate mixture in ice for thirty (30) minutes; centrifuging the iced intermediate mixture at 13,000 g for 20 minutes at 4° C. to obtain pellets; washing the pellets twice with cold acetone; resolving protein in the urine on 8-15% gradient SDS-PAGE and silver staining or blotting onto PVDF membrane; determining a level of the pro-u-plasminogen activator present in the urine; and determining a loss or absence of pro-u-plasminogen activator in the urine.