Patent ID: 8703483

Claim:
A method for identifying one or a plurality of cellular metabolites having a molecular weight of from about 10 to about 1500 Daltons that is differentially produced in human embryonic stem cells (hESCs) or human pluripotent stem cells contacted with a test compound from a population of secreted cellular metabolites, the method comprising the steps of: a) culturing hESCs or human pluripotent stem cells in the presence or absence of a test compound; b) separating members of the population of cellular metabolites having a molecular weight of from about 10 to about 1500 Daltons that are secreted from hESCs or human pluripotent stem cells; c) detecting one or a plurality of differentially produced cellular metabolites having a molecular weight of from about 10 to about 1500 Daltons from hESCs or human pluripotent stem cells; and d) identifying at least one cellular metabolite having a molecular weight of from about 10 to about 1500 Daltons that is differentially produced in cells cultured in the presence of the test compound.