Patent ID: 8088596

Claim:
A method of detecting a microorganism in a sample comprising: (a) providing (1) a biosensor device for detecting the microorganism in the sample comprising a solid substrate having a surface covalently bound to a capture agent having a saccharide moiety, (2) the sample, and (3) an unbound lectin capable of binding to the microorganism and to the saccharide moiety of the capture agent; (b) applying a polyethylene glycol thiol as a blocking agent to the solid substrate prior to applying the sample to the solid substrate to reduce nonspecific adsorption to the solid substrate; (c) applying the sample and the unbound lectin to the solid substrate with the capture agent having the saccharide moiety, for a time to bind the lectin to the saccharide moiety of the capture agent and the lectin to the microorganism to attach the microorganism to the solid substrate; and (d) detecting the microorganism attached to the solid substrate with the biosensor device.