Patent ID: 8221981

Claim:
A composition comprising at least one or a combination of Rev T7 promoter primers selected from the group consisting of: (SEQ ID NO: 24) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGGACCCACC TCCCAACC, (SEQ ID NO: 25) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGGACCCACC TCCCAGCC, (SEQ ID NO: 26) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGGACCCACC TCCCAGTT, (SEQ ID NO: 27) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGGACCCTTA CCCCAACC, (SEQ ID NO: 28) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGGACCCTTA CCCCAAAC, (SEQ ID NO: 29) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGGACCCTTA CCCCAAGC, (SEQ ID NO: 17) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGACCC ACCT, (SEQ ID NO: 18) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGACCC GCCT, (SEQ ID NO: 19) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGACCC TCCT, (SEQ ID NO: 20) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGACCC ATAC, (SEQ ID NO: 21) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGATCC ATAC, (SEQ ID NO: 22) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGACCC TTAC, and (SEQ ID NO: 23) N 1 N 2 N 3 N 4 N 5 TAATACGACTCACTATAGGN 6 AGACCACCATGTCAGACCC CTAC; wherein N 6 is G, A, C or T; and either N 1 is G, N 2 is A, N 3 is A and N 4 and N 5 are T or each of N 1 through N 5 are absent.