Patent ID: 8530634

Claim:
A method for linking a reporter moiety to an oligonucleotide, comprising: reacting a reporter moiety having an alkylazide group (Y—X—N 3 ) with an alkynyl substituted reactant (Z—W—C≡C—R 1 ) coupled to a solid support, thereby forming an azole-cycle represented by structural formula 4: wherein X is an aliphatic linker having a length of 1-20 atoms, where chain is comprised of carbon atoms, optionally substituted by one or more hetero atoms selected from the group consisting of nitrogen, oxygen and sulfur; Y is reporter molecule; W is a chain of atoms having a length of 1-20 atoms, where chain is comprised of carbon atoms, optionally substituted by one or more heteroatoms selected from the group consisting of nitrogen, oxygen and sulfur; Z is controlled pore glass (CPG), polystyrene, or oligonucleotide chain attached to solid support; and R 1 is hydrogen or C 1 -C 20 alkyl.