Patent ID: 8383348

Claim:
A method for detecting a target sequence comprising a first and second target domain in a sample, said method comprising: a) hybridizing said target sequence to a precircle probe to form a first hybridization complex, said precircle probe comprising: i) a first targeting domain; ii) a second targeting domain; iii) at least a first universal priming site; and iv) a cleavage site; wherein said first and second targeting domains hybridize to said first and second target domains; b) contacting said first hybridization complex with a ligase to form a closed circular probe; c) cleaving said closed circular probe at said cleavage site to form a cleaved probe; d) amplifying said cleaved probe to form a plurality of amplicons; and e) detecting said amplicons to detect the presence of said target sequence in said sample, wherein said sample is a purified sample.