Patent ID: 8101361

Claim:
A method to assess response to antibiotic treatment of acute pulmonary exacerbation in a human subject having cystic fibrosis comprising: a) detecting the level of mRNA expression of CD64, IL32, PLXND1, HCA112, TLR2, and CD163 genes in a PBMC sample isolated from a blood sample taken from the subject before administering the treatment; b) detecting the level of mRNA expression of CD64, IL32, PLXND1, HCA112, TLR2, and CD163 genes in a PBMC sample isolated from a blood sample taken from the subject after administering the treatment; c) comparing the level of mRNA expression of the genes from step (a) in the sample taken from the subject before administering the treatment to the mRNA level of the expression of the genes from step (b) in the sample taken from the subject after administering the treatment; and d) predicting that the subject will respond to the treatment if there is a difference in the mRNA expression levels of CD64, PLXND1, HCA112, TLR2, CD163 and IL32 genes from step (a) compared to the mRNA expression levels of the same genes from step (b).