Patent ID: 7968317

Claim:
A method of detecting 5T4 RNA or beta-human chorionic gonadotropin (beta-HCG) RNA in blood plasma or serum from a woman with a gestational trophoblastic neoplasia that is said 5T4 RNA or beta-HCG RNA-expressing neoplasia comprising the steps of: a) extracting extracellular RNA from blood plasma or serum from a woman having a gestational trophoblastic neoplasia that is 5T4 RNA or beta-HCG RNA-expressing neoplasia, wherein a portion of the extracellular RNA comprises said 5T4 RNA or beta-HCG RNA; b) amplifying a portion of the extracellular RNA or cDNA prepared therefrom and producing an amplified product, wherein the amplification is performed in either a qualitative or quantitative fashion using oligonucleotide primers specific for said 5T4 RNA or cDNA produced therefrom, or for said beta-HCG RNA or cDNA produced therefrom; and c) detecting said 5T4 RNA in the blood plasma or serum from said woman by detecting the amplified product when the amplification is performed using said oligonucleotide primers specific for said 5T4 RNA or cDNA produced therefrom, or detecting said beta-HCG RNA in the blood plasma or serum from said woman by detecting the amplified product when the amplification is performed using said oligonucleotide primers specific for said beta-HCG RNA or cDNA produced therefrom.