Patent ID: 8329416

Claim:
A kit for determining the amount of busulfan in a patient's sample by means of a busulfan immunoassay comprising a plurality of standards in separate containers, each of the standards containing a different predetermined concentration or amount of an amide of the formula: and being packaged in separate containers and reagents for carrying out said busulfan immunoassay, said reagents being packaged in containers separate from those of said standards, wherein said reagents for carrying out said immunoassay comprise two separate containers, one of which containing an antibody which is selectively reactive with busulfan and not substantially cross-reactive with tetramethylene and tetrahydrothiophene-3-ol-1,1-dioxide and the other reagent being a conjugate of a polyamine polymer with a compound of the formula: wherein A is lower alkylene; X′ is a functional linking group; Y is an organic spacing group; X is a terminal functional group capable of binding to said polyamine polymer; p and z are independent integers from 0 to 1; or a compound of the formula wherein A′ is lower alkyl or lower alkenylene.