Patent ID: 8676511

Claim:
A method of diagnosing liver fibrosis in a patient, said method comprising: a) assaying the concentration of markers in a sample from said patient, wherein said markers are α2-macroglobulin, γ-glutamyl transferase (GGT), bilirubin, and hyaluronic acid; b) determining, with a suitably programmed computer, an end value, H, using an algorithm, wherein said algorithm is, H=y /(1+ y ) wherein, y =exp(− X −( C 1 *age)+( C 2 *gender)+( C 3 *α2-macroglobulin)+( C 4 *hyaluronic acid)+( C 5 *bilirubin)−( C 6 *GGT)) wherein, X is between −6.8460 and −1.5257 inclusive, C 1 is between −0.0877 and 0.0378 inclusive, C 2 is between 0.3064 and 1.7992 inclusive, C 3 is between 0.4406 and 1.5672 inclusive, C 4 is between 0.0090 and 0.0515 inclusive, C 5 is between −0.0099 and 0.1482 inclusive, C 6 is between −0.0055 and 0.0032 inclusive, and wherein, age is in years, male gender=1, female gender=0, α 2 -macroglobulin is in g/L, hyaluronic acid is in μg/L, bilirubin is in μmol/L, GGT is in U/L; and c) comparing said end value, H, to a cut-off value associated with the presence of liver fibrosis and diagnosing said patient with liver fibrosis when the end value is greater than or equal to the cut-off value.