Patent ID: 7166298

Claim:
A method for producing monoclonal antibodies, comprising: (a) mixing at least one cationic lipid with a polynucleotide, thereby forming a cationic lipid-polynucleotide complex, wherein said polynucleotide comprises a promoter operably linked to a DNA sequence coding for an immunogen; (b) administering said cationic lipid-polynucleotide complex to at least one mouse, thereby generating at least one immunized mouse; (c) removing B-lymphocytes from said at least one immunized mouse; (d) fusing said B-lymphocytes from said at least one immunized mouse with myeloma cells, thereby producing hybridomas; (e) cloning said hybridomas; (f) selecting positive clones which produce anti-immunogen antibody; (g) culturing said anti-immunogen antibody-producing clones; and (h) isolating anti-immunogen antibodies from said cultures.