Patent ID: 8101378

Claim:
A kit comprising a vector for expressing a protein in bacterial and insect host cells, the vector comprising: first and second promoters, wherein the first promoter is effective to express a downstream protein coding sequence in insect host cells and the second promoter is effective to express the same downstream protein coding sequence in bacterial host cells; and a cloning site downstream of the promoters such that a protein coding sequence inserted into the site is transcribed in insect host cells and in bacterial host cells, wherein the second promoter is located between the first promoter and the cloning site, and the distance between the 3′ end of the second promoter and a ribosome binding site (RBS) is less than 100 nucleotides; further comprising at least one item selected from the group consisting of: bacterial host cells, insect host cells, baculovirus DNA, transfection reagent, agarose, an inducer, a restriction enzyme, a ligation mix, culture medium, an antibody, a buffer, a control plasmid, and instructions for use.