Patent ID: 7993925

Claim:
A method for inhibiting expression of a target gene in a cell in an animal comprising: (a) delivering a nucleic acid construct to an isolated cell so as to obtain a transformed cell harboring only a single, stably integrated copy of the construct, wherein said nucleic acid construct contains only a single tetracycline responsive RNA Polymerase II (Pol II) promoter operably linked to a sequence encoding only a single synthetic miR30-based shRNA directed against a target gene, (b) culturing the transformed cell so as to obtain a population of transformed cells harboring only a single, stably integrated copy of the construct, (c) implanting the transformed cells into an animal, and (d) inducing expression of the miR30-based shRNAso as to inhibit expression of the target gene by at least about 80% in the implanted, transformed cells.