Patent ID: 8268583

Claim:
A method for enhancing secretion of heterologous exoprotein of interest, the method comprising expressing said heterologous exoprotein in a recombinant Bacillus cell, wherein the cell comprises a nucleic acid construct encoding the heterologous exoprotein of interest and: a) a heterologous promoter operably linked with at least one gene encoding metallo regulated gene A (MrgA) protein with an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2; or b) at least one heterologous gene encoding MrgA protein with an amino acid sequence having at least 95% sequence identity to SEQ ID NO:2, wherein the secretion of the heterologous exoprotein and MrgA is increased compared to an otherwise isogenic Bacillus cell without a) or b), and wherein the exoprotein is not galactosidase.