Patent ID: 8852902

Claim:
A method of producing a trimethylpentanoic acid, comprising: providing a prokaryotic host cell and culturing said host cell in a suitable culture medium such that the trimethylpentanoic acid is produced, wherein the host cell comprises a recombinant nucleic acid encoding a hybrid polyketide synthase (PKS) having a module from two or more PKS polypeptides, and further, wherein the hybrid PKS comprises: a loading module comprising an acyltransferase (AT) and an acyl carrier protein (ACP) that uses pivaloyl-CoA as a starter unit, and a module comprising a ketosynthase (KS), methylmalonyl-CoA acyltransferase (mmAT), dehydratase (DH), enoylreductase (ER), ketoreductase (KR), and ACP that uses 2-methylmalonyl-CoA as an extending unit; and a thioesterase (TE).