Patent ID: 8062869

Claim:
A method of producing L-lysine comprising: A) culturing in a medium an Escherichia coli bacterium in which: (a) the intracellular activities of all of the following enzymes are enhanced: dihydrodipicolinate synthase which comprises a mutation replacing at least one amino acid selected from the group consisting of the alanine at the 81st position and the histidine at the 118th position with another amino acid, wherein the amino acid sequence of said dihydrodipicolinate synthase prior to said mutation(s) is the amino acid sequence of wild-type dihydrodipicolinate synthase of Escherichia coli, aspartokinase, dihydrodipicolinate reductase, phosphoenolpyruvate carboxylase, nicotinamide adenine dinucleotide transhydrogenase, and aspartate-semialdehyde dehydrogenase; and (b) the intracellular activities of at least one of the following enzymes are enhanced: i) diaminopimelate dehydrogenase, or ii) tetrahydrodipicolinate succinylase and succinyldiaminopimelate deacylase, wherein said activities are enhanced by a method selected from the group consisting of a) introducing a promoter or promoters which are operably linked to the gene encoding the enzymes into the chromosome of said bacterium, b) increasing the copy number of the genes encoding the enzymes by introducing one or more expression vectors into said bacterium, and c) combinations thereof; B) allowing L-lysine to accumulate in said medium or said bacterium; and C) collecting L-lysine from the medium or the bacterium.