Patent ID: 8716023

Claim:
A method for reducing or eliminating the expression of a target gene encoding a biological substance in a filamentous fungal strain, comprising: (a) inserting into the genome of an Aspergillus niger , an Aspergillus oryzae , or a Trichoderma reesei strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a first homologous transcribable region of the target gene encoding the biological substance and a second nucleotide sequence comprising a second homologous transcribable region of the target gene, wherein the first and second homologous regions are complementary to each other and the second homologous region is in reverse orientation relative to the first homologous region; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the Aspergillus niger , the Aspergillus oryzae , or the Trichoderma reesei strain under conditions conducive for production of the interfering RNA, wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene encoding the biological substance.