Patent ID: 8236499

Claim:
A method of producing a population of linear single stranded nucleic acids, the method comprising: providing a double-stranded genomic DNA, cDNA, or DNA concatamer; cleaving the genomic DNA, the cDNA, or the concatemer, thereby producing linear double-stranded nucleic acids; ligating exonuclease-sensitive tags to the 5′ ends of the first strands of the linear double-stranded nucleic acids and ligating exonuclease-resistant tags to the 5′ ends of the second strands of the linear double-stranded nucleic acids, thereby generating double-stranded fragments from the genomic DNA, the cDNA, or the concatamer, wherein first strands of the fragments are exonuclease-sensitive strands and second strands of the fragments are exonuclease-resistant strands; and eliminating the first strands from the double-stranded fragments by digesting the first strands with an exonuclease, thereby producing the population of linear single-stranded nucleic acids.