Patent ID: 8685644

Claim:
Method for the determination of the concentration of ligands contained in a sample to be analyzed, comprising the steps of, immobilizing on a support receptors that can enter into a specific bond with the ligands, bringing the sample into contact with the receptors, obtaining at least one measurement value representing the frequency of the bonds between the ligands and the receptors, and determining on the basis of this measurement value the concentration of the ligands in the sample, wherein at least one measurement value of the surface occupation of the surface of the support with the receptors is determined, and that the concentration of the ligands is determined by means of the measurement values for the surface occupation and the frequency of the bonds; wherein the receptors are not bonded to ligands during the measurement of the surface occupation, and that the sample is brought into contact with the receptors after the surface occupation of the surface of the support with the receptors has been determined; wherein after the immobilization of the receptors on the surface of the support, for the determination of the measurement value for the surface occupation, the electrical impedance is measured on the surface of the support, and that then the sample is brought into contact with the receptors and the at least one measurement value for the frequency of the bonds is determined, and wherein the at least one measurement value for the frequency of the bonds between the ligands and receptors is determined by a luminescent radiation method wherein luminescent radiation is activated by an optical radiation source and wherein the concentration of ligands is determined by the formula: Ligand concentration= K ( M/F ) wherein M represents the frequency of the receptor ligand complexes, F represents the surface occupation of the surface of the support with the receptors, and K is the bonding constant, further wherein said support is a semiconductor substrate comprising a plurality of detectors and a plurality of surface occupation sensors together arranged in a matrix pattern comprising an alternating pattern of said plurality of detectors and said plurality of surface occupation sensors, whereby each measurement point formed by each receptor comprises therebelow at least one detector and at least one surface occupation sensor, said plurality of detectors being used to obtain at least one measurement value representing the frequency of the bonds between the ligands and the receptors.