Patent ID: 7091035

Claim:
A cell culture system comprising: a microscopic viewing apparatus; a cell culture chamber including a holder having a recess, and an insert member received in the holder recess, the insert member having first and second inlet ports, a single outlet port, a side port and an interior volume in fluid communication with said first and second inlet ports and said single outlet port in which cells are cultured; a first inlet tubing member received in the first inlet port, the first inlet tubing member being connected to a gas source for controlling the flow of gas to the interior volume; a second inlet tubing member received in the second inlet port, the second inlet tubing member being connected to a medium source for controlling the flow ofuiedium to the interior volume; a single outlet tubing member received in the outlet port for removing gas and medium from the interior volume, wherein the insert member is constructed such that the gas and medium introduced into the interior volume only exit through said single outlet port; and an electrode disposed in the side port for manipulating the cells contained in the interior volume, the electrode having one end that is movable in the interior volume for manipulating the cells; wherein the cell culture chamber and the microscopic viewing apparatus are arranged such that the viewing apparatus can view cells being cultured in the chamber interior volume.