Patent ID: 7169548

Claim:
A method of sperm cell processing, comprising the steps of: obtaining sperm cells from a male of a mammalian species; cryopreserving said obtained sperm cells; thawing said cryopreserved sperm cells; sorting said thawed sperm cells into X chromosome-bearing sperm cells and Y chromosome-bearing sperm cells; and cryopreserving said sorted sperm cells to yield twice-cryopreserved sorted sperm cells; wherein an increased percentage of said twice-cryopreserved sorted sperm cells, when thawed, are motile, compared to said thawed sperm cells, and wherein said twice-cryopreserved sorted sperm cells, when thawed, yield blastocysts at an increased rate, compared to said thawed sperm cells, when used to fertilize eggs from a female of said mammalian species.