Patent ID: 7052881

Claim:
A method of producing a recombinant nucleic acid molecule, said method comprising: providing a complementing cell comprising at least an adenoviral EIA gene; introducing a precursor molecule into said complementing cell, wherein said precursor molecule is a nucleic acid molecule based on or derived from an adenovirus, said precursor molecule has one functional inverted terminal repeat, said precursor molecule comprises all other adenovirus derived genetic information necessary for replication not present in said complementing cell, and said precursor molecule is in a linear and essentially single stranded form and comprises, at the precursor molecule's 3′ terminus, a recombinantly fused sequence complementary to an upstream part of the same strand of the precursor molecule, to allow said recombinantly fused sequence and said upstream part to form base pairs and function as a start-site for a nucleic acid polymerase; and producing a recombinant nucleic acid molecule by the action of said nucleic acid polymerase on said precursor molecule in said complementing cell.