Patent ID: 8895276

Claim:
A microorganism variant having the ability to produce alkanes selected from the group consisting of pentadecane, heptadecane, octane, and nonane, wherein a gene encoding acyl coenzyme A dehydrogenase and a fadR gene encoding a DNA-binding transcriptional dual regulator are deleted or attenuated; and a gene encoding an acyl-CoA thioesterase, an atoDA gene encoding an acetyl-CoA: acetoacetyl-CoA transferase, a gene encoding a fatty acyl-CoA reductase, a gene encoding a fatty aldehyde decarbonylase, and an atoE gene encoding a short-chain fatty acid outer membrane transporter are introduced or amplified; and the native promoter of the gene encoding acyl-CoA synthetase and the attenuator-containing native promoter of the gene encoding the fatty acid outer membrane transporter are further substituted with a strong promoter selected from the group consisting of trc promoter, tac promoter, T7 promoter, lac promoter, and trp promoter.