Patent ID: 8187885

Claim:
A calibration kit comprising: a. a set of populations of microbeads, the microbeads having a surface and being made of a particular material; b. each of said microbead populations having a median fluorescence intensity within a linear detection range of a fluorescence detector to be calibrated; c. each of said microbead populations having spectral properties that are sufficiently different from any other microbead populations in the same set to allow discrimination thereof; d. at least two microbead populations of said set containing a different and determinable quantity of one or more non-overlapping internally-bound fluorophores measurable by a fluorescence detector to be calibrated; e. at least one microbead population of said set, having bound to the surface of each microbead, either directly or indirectly, a determinable quantity of one or more non-overlapping fluorophores measurable by a fluorescence detector to be calibrated; and f. at least one unlabeled microbead population of said set, to serve as a control for autofluorescence of said material.