Patent ID: 8853134

Claim:
A microarray for detecting a plurality of nucleic acid analytes in a complex sample, comprising a plurality of non-naturally occurring binary nucleic acid probe complexes immobilized at separate sites on a solid support, wherein each complex comprises a binary nucleic acid probe comprising two oligonucleotide strands that generate a detectable signal when each strand is bound to one of the plurality of analytes; wherein the binary nucleic acid probe is a non-naturally occurring nonenzymatic reporter oligonucleotide-binding binary nucleic acid probe for detecting an oligonucleotide analyte comprising two oligonucleotide strands wherein 1. a first oligonucleotide strand comprises: a. at its 5′-terminus a reporter oligonucleotide-binding arm that is complementary to and selectively hybridizes with a reporter oligonucleotide to which a reporter that can be detected is bound, and b. at its 3′-terminus an analyte-binding arm that is complementary to and selectively hybridizes with a first region of the oligonucleotide analyte, and 2. a second oligonucleotide strand comprises: a. at its 3′-terminus a reporter oligonucleotide-binding arm that is complementary to and selectively hybridizes with a reporter oligonucleotide to which a reporter that can be detected is bound, and b. at its 5′-terminus, an analyte-binding arm that is complementary to and selectively hybridizes with a second region of the oligonucleotide analyte; and wherein the analyte binding arm of one strand of each of the plurality of binary probe complexes is covalently bound to the solid support by a linker.