Patent ID: 8728728

Claim:
A method for amplifying a plurality of different target sequences within a sample, comprising: a) amplifying within a single amplification reaction mixture between one hundred and twelve thousand different target sequences from a sample including a plurality of different target sequences, wherein the amplifying includes contacting at least some portion of the sample with a plurality of target-specific primers and a polymerase under amplification conditions, thereby producing a plurality of different amplified target sequences, wherein a plurality of the target-specific primers include a cleavable group and at least one of the plurality of amplified target sequences includes a primer-derived sequence containing the cleavable group; b) cleaving the cleavable group of the primer-derived sequence of the at least one amplified target sequence, thereby forming a cleaved end of the at least one amplified target sequence; and c) ligating at least one double-stranded adapter to the cleaved end of the at least one amplified target sequence in a blunt-ended ligation reaction, thereby producing one or more double-stranded adapter-ligated amplified target sequences.