Patent ID: 7407807

Claim:
A method for producing a chimaeric polypeptide comprising human papillomavirus (HPV) L1 polypeptide containing a heterologous antigenic peptide inserted therein, the method comprising the steps of: introducing a DNA sequence encoding a heterologous antigenic peptide into a DNA sequence encoding a full-length L1 polypeptide wherein the nucleotides encoding the heterologous antigenic peptide replace a corresponding number of nucleotides within the DNA sequence encoding the L1 polypeptide thereby producing a DNA encoding a chimaeric polypeptide; and wherein said replaced nucleotides within the DNA sequence encoding the L1 polypeptide are: 241-279, 391-429, 520-558, 1240-1278 or 1291-1329 of SEQ ID NOs: 12, 6, 4, 10, and 8, respectively; introducing the DNA sequence encoding the chimaeric polypeptide into a host cell in which the DNA sequence can be expressed; causing expression of the DNA sequence; and recovering the produced chimaeric polypeptide.