Patent ID: 8301398

Claim:
A method of identifying a compound that binds insulin receptor (IR), comprising: (a) obtaining a crystal comprising IR selected from the group consisting of: (i) an orthorhombic crystal of IR ectodomain comprising a protein consisting of residues 1-485 of SEQ ID NO:1 in space group P2 1 2 1 2 1 having a cell unit dimension of a=103.86 Angstrom, b=130.24 Angstrom, and c=160.92 Angstrom, and (ii) an orthorhombic crystal of a complex consisting of IR-delta-beta (IRΔβ) of SEQ ID NO:15, Fab 83-7 of SEQ ID NOs:8 and 10, and Fab 83-14 of SEQ ID NOs:4 and 6 in space group C222 1 having a unit cell dimension of a=123.0 Angstrom, b=319.7 Angstrom, and c=204.9 Angstrom; (b) determining the three-dimensional structure of the IR by X-ray diffraction to obtain the atomic coordinates of Appendix I and or II; (c) contacting an IR structure defined by the atomic coordinates of Appendix I and/or Appendix II, or a subset thereof with a test compound; and detecting an interaction between the compound and the atomic coordinates, wherein an energetically favored interaction between the test compound and the atomic coordinates is indicative of a compound that binds IR.