Patent ID: 7749724

Claim:
A plating medium for growth and detection of Enterobacter sakazakii having α-glucosidase activity, comprising: a basal medium comprising a gel forming constituent, between about 2 g/L and 40 g/L Tryptone, and between about 0.5 g/L and about 5 g/L bile salts, the medium capable of supporting growth of Enterobacter sakazakii ; and at least one compound, or salt thereof, that is an α-glucosidase substrate suitable to produce fluorescence when exposed to a microorganism having α-glucosidase activity, wherein the compound is of formula (I) wherein R 1 , R 2 , R 3 , R 4 and R 5 are independently: C 1 -C 4 linear or branched alkyl groups, optionally containing an oxygen atom in the alkyl chain; C 1 -C 4 linear or branched alkoxy; nitro; carboxy, C 1 -C 4 linear or branched carboxyalkyl; and cyano; and wherein the alkyl groups may include one or more halogen atoms (e.g., fluorine, chlorine and bromine), as substituents.