Patent ID: 7919257

Claim:
A method for selecting a single recombinant cell that expresses a heterogeneous combination of monospecific and bispecific antibodies, or antibody fragments thereof, wherein the monospecific and bispecific antibodies, or antibody fragments thereof, are human, humanized, or deimmunized, and wherein the heterogeneous combination has specific affinity for two target epitopes, said method comprising: carrying out a process for producing the heterogeneous combination; the process comprising providing three different variable regions consisting of two heavy chain regions and one light chain variable region in recombinant cells, wherein antigen binding parts of the variable regions originate from an antibody from a single species and wherein one variable region is able to functionally pair with more than one other variable region, and under conditions allowing for pairing of variable regions and secretion of the paired regions from the recombinant cells resulting in the production of said heterogeneous combination, and providing two target epitopes, selecting a single recombinant cell from said recombinant cells that produces a heterogeneous combination that binds the two target epitopes, and co-purifying the heterogeneous combination therefrom utilizing a shared feature of each of the monospecific and bispecific antibodies, or antibody fragments thereof.