Patent ID: 7355095

Claim:
A method of producing an oviparous fish with fluorescence specifically expressed only in the heart with no expression in other tissues, the method comprising: (a) constructing a plasmid including a first ITR, a regulatory region, a gene encoding a fluorescent protein, an SV40 poly A and a second ITR, in order from upstream to downstream, wherein the regulatory region is operably linked to the gene encoding a fluorescent protein and is that set forth in SEQ ID NO: 8 (b) linearizing the plasmid construct; (c) microinjecting the linearized plasmid construct into a fertilized egg of the oviparous fish; (d) incubating the microinjected egg to form an embryo; (e) selecting an incubated embryo that exhibits fluorescence; and (f) cultivating the selected embryo to maturity to produce the fish having fluorescence specifically expressed in the heart, with no expression in other tissues.