Patent ID: 8105478

Claim:
A method for measuring at least one of a concentration and change in concentration of a redox-active substance as a mediator in a molecular-biological detection system, in which as a result of application of suitable potentials to a working electrode, at least one of a reduction process and an oxidation process takes place as a redox reaction, the method comprising: measuring an oxidation current to obtain a measuring phase; measuring a reduction current to obtain a relaxation phase; pulsing the potential of the working electrode, and alternately forming the measuring phases and the relaxation phases; selecting measuring-phase pulse lengths so that, at the end of the pulse, a capacitive current is small in comparison with a Faraday current; and selecting relaxation-phase pulse lengths so that, at the end of the pulse, a concentration gradient is relaxed such that at a beginning of a following measuring phase, the change in concentration of the mediator, brought about by the measurement of the mediator, is reversible.