Patent ID: 8293084

Claim:
A method for an automated determination and/or identification of carbohydrates and/or carbohydrate mixture composition pattern profiling comprising the steps of: a) obtaining a sample containing one or more carbohydrate(s); b) labeling said one or more carbohydrate(s) with a fluorescent label; c) adding at least one standard of known composition to the sample containing the one or more carbohydrate(s); d) determining migration time(s) of said one or more carbohydrate(s) and the standard of known composition using capillary gel electrophoresis-laser induced fluorescence; e) normalizing the migration time(s) to migration time indice(s) based on given standard migration time indice(s) of the standard; f) comparing said migration time indice(s) of the one or more carbohydrate(s) with standard migration time indice(s) from a database; and g) identifying or determining the one or more carbohydrate(s) and/or carbohydrate mixture composition patterns, whereby at least two orthogonal standards are added in step c) and orthogonal cross normalization is performed in step e) based on the given standard migration time indices of the at least two orthogonal standards.