Patent ID: 8666678

Claim:
A sequencing method, comprising: (a) flowing nucleotides onto a sensor array adapted for sequencing, the sensor array including a first well containing a polynucleotide strand; (b) receiving from the sensor array signal data relating to chemical reactions resulting from the flow of nucleotides, the signal data comprising errors due to phase synchrony loss; (c) determining, using the signal data, sequence information of at least a portion of the polynucleotide strand; (d) constructing a phase-state model for a set of flows that encompasses the sequence information, wherein the model includes a signal correction parameter and wherein the model is stored in a machine-readable memory; (e) calculating, using the phase-state model, predicted signals resulting from the set of nucleotide flows; (f) comparing the predicted signals to the signal data; (g) adjusting the signal correction parameter of the phase-state model based on the comparison of the predicted signals to the signal data; and (h) storing the adjusted signal correction parameter in the memory.