Patent ID: 7700285

Claim:
A method of recovering recombinant protein expressed by open reading frame 2 of PCV2 from the supernate of unlysed insect cells comprising the steps of: A) cloning said recombinant open reading frame 2 from PCV2 into a transfer vector; B) transfecting the portion of said transfer vector containing said recombinant open reading frame 2 into a baculovirus; C) infecting insect cells in media with said baculovirus; D) causing said baculovirus to express the protein from said open reading frame 2 in insect cells; E) separating said insect cells from the supernate; and F) recovering said expressed open reading frame 2 protein in said supernate of unlysed cells at least 5 days after the insect cells are infected with said baculovirus wherein the amount of said open reading frame 2 protein that is recovered after 5 days is at least 41.33 μg/ml.