Patent ID: 8268636

Claim:
A device for detecting an analyte in a liquid sample deposited on a first portion of the device for transport to a second portion of the device, wherein the device comprises a biphasic substrate comprising: A) a release medium formed of a first material and comprising: i) a labeled conjugate comprising a binding member reactive with a first epitope of the analyte and a label; and ii) a biotinylated capturable component having a site reactive with a second epitope of the analyte, such that if the analyte is present in the sample, the analyte produces a complex comprising the labeled conjugate, the analyte for detection, and the biotinylated capturable component; and B) a capture medium in fluid communication with the release medium and formed of a second, different material, the capture medium comprising a capture site for capturing the complex, the capture site having immobilized thereon a capture component comprising polymerized streptavidin.