Patent ID: 7455973

Claim:
A method for diagnosing high-grade cervical disease in a patient independent of the patient's HPV infection status comprising detecting overexpression of at least one nucleic acid molecule that encodes a nuclear biomarker that is selectively overexpressed in high-grade cervical disease, wherein the nuclear biomarker is selected from the group consisting of p14ARF, p21 waf1 , Topo2A, and Cyclin E, the method comprising: (a) obtaining a body sample from the patient; (b) isolating nucleic acid material from the sample; (c) mixing said nucleic acid material with at least one pair of oligonucleotide primers specific for the nuclear biomarker and a thermostable DNA polymerase under conditions that are suitable for amplification by polymerase chain reaction (PCR); d) performing PCR; and, e) detecting PCR amplification products.