Patent ID: 7993935

Claim:
A test strip for immunochromatography for detecting a first analyte and a second analyte in a sample, comprising: a sample receiving member for receiving the sample; a label holding member for holding a first labeling substance specifically binding to the first analyte and a second labeling substance specifically binding to the second analyte; and a chromatography membrane comprising a first detection zone which comprises a first substance specifically binding to the first analyte and a second detection zone which comprises a blocking material and a second substance specifically binding to the second analyte, wherein the second detection zone is formed by a mixture of the blocking material and the second substance, the sample receiving member is arranged in contact with the label holding member, and the chromatography membrane is arranged in contact with the sample receiving member, the label holding member, or both, wherein the chromatography membrane is blocked by immersing in a solution comprising the blocking material after forming the first detection zone and the second detection zone, wherein an amount of the blocking material included in the second detection zone is larger than an amount of the blocking material included in the chromatography membrane other than the second detection zone, and wherein the blocking material is selected from the group consisting of globulin, albumin, casein, gelatin, skimmed milk, polyvinyl alcohol, polyvinylpyrrolidone and a serum collected from animals.