Patent ID: 7932083

Claim:
A DNA participating in biological transformation of a macrolide compound (hereinafter referred to as a macrolide compound 11107B) represented by the formula (I): into a 16-position hydroxy macrolide compound represented by the formula (II): the DNA being an isolated and pure DNA comprising a DNA encoding a protein having 16-position hydroxylating enzymatic activity which is characterized by the following (a), (b), or (c): (a) a DNA encoding a protein having the enzymatic activity to hydroxylate the 16-position of the macrolide compound 11107B, wherein the DNA is selected from the group consisting of (1) a continuous nucleotide sequence from base 1322 to base 2548 of SEQ ID NO: 1; (2) a continuous nucleotide sequence from base 420 to base 1604 of SEQ ID NO: 4; and (3) a continuous nucleotide sequence from base 172 to base 1383 of SEQ ID NO: 7; (b) a DNA which has a nucleotide sequence having 90% or more identity over the full length sequence with the DNA described in (a); (c) a DNA encoding a protein having the same amino acid sequence as the protein encoded by the DNA described in (a) or (b) though it does not have 90% or more identity with the DNA described in (a) because of the degeneracy of a gene codon.