Patent ID: 7598076

Claim:
A cell culture insert ( 1 ) comprising a beaker-shaped insert wall ( 11 ) having a membrane filter bottom ( 9 ), a top, and projecting support arms ( 3 , 4 ) that are distributed around the circumference of the top and having lateral spacers ( 7 , 8 ) for a vertical and horizontal orientation in a well ( 2 ) with a liquid culture medium (N) in a cell culture plate, characterized in that the spacers ( 7 , 8 ) are distributed around the circumference of the cell culture insert ( 1 ) and have different lengths to the insert wall ( 11 ) including a shortest spacer ( 8 ), in such a way that one large feed window ( 5 ) and multiple smaller windows ( 5 A) are created when the insert ( 1 ) is in rest position in the well ( 2 ); wherein the shortest spacer ( 8 ) has a knob shape.