Patent ID: 7935800

Claim:
A method for detecting the presence of a target nucleic acid comprising: a) forming an invasive cleavage structure, said invasive cleavage structure comprising an RNA target nucleic acid; b) cleaving said invasive cleavage structure with a cleavage agent comprising an isolated composition comprising the polypeptide of SEQ ID NO:2668 to produce a non-target cleavage product; and c) detecting the cleavage of said invasive cleavage structure, wherein said target nucleic acid comprises a first region and a second region, said second region downstream of and contiguous to said first region, and wherein said invasive cleavage structure comprises said target nucleic acid, a first oligonucleotide, and a second oligonucleotide, wherein at least a portion of said first oligonucleotide is completely complementary to said first region of said first target nucleic acid, and wherein said second oligonucleotide comprises a 3′ portion and a 5′ portion, wherein said 5′ portion is completely complementary to said second region of said target nucleic acid.