Patent ID: 8663993

Claim:
A dye binding method for protein analysis consisting essentially of: preparing an initial reference dye solution of unknown concentration by mixing an initial reference dye concentrate with water; creating an electronic signal based upon the absorbance of the initial reference dye solution; thereafter, creating an electronic signal based upon the absorbance of a dye filtrate solution prepared from a weighed initial protein sample homogenized in the initial reference dye solution and filtered from the homogenate; sending the absorbance signals from the reference dye solution and the dye filtrate solution to a processor that compares the respective absorbances and calculates the protein content of the weighed protein sample based upon the difference between the absorbances; creating an electronic signal based upon the absorbance of a successive dye filtrate solution prepared from a successive weighed protein sample homogenized in the initial reference dye solution and filtered from the homogenate; and sending the absorbance signal from the successive sample dye filtrate solution to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.