Patent ID: 7115400

Claim:
A method for amplification of at least one nucleic acid, comprising the following steps: (1) forming at least one nucleic acid template comprising the at least one nucleic acid to be amplified, wherein the at least one nucleic acid contains an oligonucleotide sequence Y at the 5′ end and an oligonucleotide sequence Z at the 3′ end, and the at least one nucleic acid carries a means for immobilizing the at least one nucleic acid to a solid support at the 5′ end; (2) mixing the at least one nucleic acid template, in the presence of the solid support, with one or more colony primers X, each of which can hybridize to the oligonucleotide sequence Z and carries a means for immobilizing the colony primer to the solid support at the 5′ end, whereby the 5′ ends of both the at least one nucleic acid template and the colony primers are immobilized to the solid support; wherein said 5′ ends of both the at least one nucleic acid template and the colony primers are immobilized to said solid support such that they cannot be removed by washing with water or aqueous buffer under DNA-denaturing conditions; and (3) performing one or more nucleic acid amplification reactions on the immobilized nucleic acid template, so that nucleic acid colonies are generated.