Patent ID: 7759115

Claim:
A container assembly comprising (i) a vessel for containing a biological medium as well as gametes and/or one or more embryo(s), the vessel having a wall which confines the biological medium, gametes and/or embryo(s) inside the vessel, said vessel wall including a CO 2 permeable wall; (ii) a closure device for selective access to the interior of the vessel, and (iii) a buffer gas chamber for containing a CO 2 enriched atmosphere and including a shell cooperable with said vessel, the buffer gas chamber including the shell being disposed around the vessel and the CO 2 enriched atmosphere being in communication with the CO 2 permeable wall for supplying CO 2 enriched atmosphere to the contents of the vessel such that an inner surface of the vessel wall is in contact with liquid contents of the vessel and an outer surface of the vessel wall is in contact with CO2 enriched gas of the buffer gas chamber.