Patent ID: 7978403

Claim:
A method for microscopy comprising: illuminating an object at an object plane using an illumination beam having an illumination angle to the object plane, wherein the illumination angle is one of a plurality of illumination angles; recording a sub-image at an image plane based on the illumination at the object plane, wherein the sub-image corresponds to one region of a plurality of spatial frequency regions of a frequency space; enhancing each sub-image that corresponds to each region of the plurality of spatial frequency regions that is based on each illumination angle of the plurality of illumination angles using signal processing algorithms; and combining each enhanced sub-image of the plurality of sub-images to assemble a composite image of the object, wherein the frequency space covered in the plurality of sub-images extends to (1+NA)n/λ or less, wherein NA is a numerical-aperture of an objective lens, λ is an optical wavelength of the illumination beam, and n is a refractive index of a transmission medium.