Patent ID: 8710188

Claim:
A method for determining the structure of a complex comprising a compound and a peptide bound to factor IXa protein, wherein said factor IXa is selected from the group consisting of SEQ ID NO: 14 and 18, said peptide comprises SEQ ID NO: 13 and said compound is selected from the group consisting of: said method comprising: (a) mixing the factor IXa protein with the compound; (b) crystallizing said factor IXa protein-compound complex; and (c) determining the three-dimensional structure of the complex which results in atomic coordinate data of any one of Tables 2-6 optionally varied by a root mean square deviation of conserved residue backbone atoms or of alpha carbon atoms of less than about 1.5 Å when superimposed on backbone atoms described in Table 2, 3, 4, 5 or 6, and wherein the resultant crystal of part (b) forms in one of in the following crystalline space groups and unit cell parameters: (i) space group P2 1 2 1 2 1 having unit cell dimensions a=48.1 Å, b=69.8 Å, c=92.1 Å and α=β=γ=90° or space group P4 3 2 1 2 having unit cell dimensions a=100.4 Å, b=100.4 Å, c=97.3 Å and α=β=γ=90°; (ii) space group P4 3 2 1 2 having unit cell dimensions a=100.6 Å, b=100.6 Å, c=98.1 Å and α=β=γ=90°; (iii) space group P4 3 2 1 2 having unit cell dimensions a=99.2 Å, b=99.2 Å, c=97.3 Å and α=β=γ=90°; and (iv) space group P4 3 2 1 2 having unit cell dimensions a=100.5 Å, b=100.5 Å, c=98.4 Å and α=β=γ=90°.