Patent ID: 7863014

Claim:
A process for preparing nicotinamide adenine dinucleotide phosphate (NADP) in vitro comprising performing phosphorylation using a polyphosphoric acid or a salt thereof and nicotinamide adenine dinucleotide (NAD + ) as substrates in the presence of a polyphosphate-dependent NAD + kinase from a Mycobacterium , wherein the reaction solution contains 5-15% by weight of the polyphosphoric acid or a salt thereof, 8-50 mM of NAD + and 5-150 mM of a divalent metal ion, and a pH of the reaction solution is in a range of 6-7, and wherein the NAD + kinase is a polypeptide comprising the amino acid sequence of SEQ ID NO:1, and having an optimal pH of 5.5-6.5.