Patent ID: 6991818

Claim:
A process for the preparation of an extract of seeds of Annona squamosa comprising Isosquanmocin, said process comprising: (a) disintegrating the Annona squamosa seeds into powder, (b) subjecting the powder of step (a) to continuous extraction with methanol or aqueous methanol, or ethanol or aqueous ethanol at an ambient temperature, (c) concentrating the extract of step (b) and stirring the concentrate with petroleum ether or hexane having boiling point in the range of 60–80° C. and phasing the stirred concentrate into a heavy phase and a light phase, (d) stirring the heavy phase of step (c) containing the active ingredient Isosquamocin, and optionally Squamocin-G, Squamocin-H, squamocin-M, Squamocin-L, Squamocin-C, Squamocin-J, Squamocin-K, Squamocin-B, Squamostatin-A, Squamostatin-B, Squamostatin-C or compounds derived from the plant Annona squamosa and having retention times of 5.88 minutes, 14.18 minutes, and 54.25 minutes in HPLC with suitable proportion of organic solvent and water into a mixture containing an organic phase and separating the organic phase, (e) concentrating the organic phase of step (d) to yield extract of Annona squamosa in the form of brown semisolid, (f) dissolving the semisolid from step (e) in an organic solvent and subjecting it to column chromatography over silica gel using eluants of increasing polarity to obtain a solid residue, and (g) dissolving the residue obtained from (f) and purifying the dissolved residue with methanol and water.