Patent ID: 8318460

Claim:
A method for determining a haplotype comprising at least three polymorphic markers in a subject comprising the steps of: (a) diluting a nucleic acid sample from the subject to a single nucleic acid molecule dilution; (b) amplifying in one multiplex reaction the single nucleic acid molecule dilution with at least a first, a second and a third primer pair, wherein each primer pair flanks a nucleic acid region consisting of about 50 bp, each primer pair thereby producing an amplicon consisting of about 50 bp, and wherein the at least first, second and third primer pair each are designed to amplify a different nucleic acid region designated as a first, a second and a third nucleic acid region; (c) genotyping the polymorphic site in the at least first, second, and third nucleic acid region using primer extension and mass spectrometric detection thereby resulting in at least a first, a second and a third genotype; and (d) determining the haplotype comprising polymorphic markers from the at least the first, the second and the third genotype to obtain a haplotype for the subject.