Patent ID: 6949352

Claim:
A method for screening molecules which inhibit the activity of native protein of a parasitic organism of the Apicomplexa phylum, having type 2C serine-threonine phosphatase activity, wherein said method comprises: a) fixing a native or a recombinant Toxofilin of said parasitic organism to a matrix; b) phosphorylating said Toxofilin with labeled ATP using a parasite kinase fraction or a recombinant casein kinase II; c) controlling the phosphorylation of Toxofilin by labeling counting the phosphorylated and labeled Toxofilin; d) incubating the labeled Toxofilin with or without the molecule to be tested and adding a type 2C serine-threonine phosphatase of said parasitic organism; and e) measuring the labeling; wherein a variation of the labeling of the Toxofilin incubated with the molecule to be tested compared with the labeling of the Toxofilin incubated without the molecule to be tested is indicative of the capacity of the molecule to inhibit said type 2C serine-threonine phosphatase activity of said protein.