Patent ID: 6939702

Claim:
A lipase which is a polypeptide having an amino acid sequence which has at least 90% identity to SEQ ID NO:1 and which comprises a substitution of an electrically neutral or negatively charged amino acid with R or K at a position corresponding to any of positions 3, 224, 229 and 233 in SEQ ID NO:1 and comprises: i) a negative amino acid at a position corresponding to position E210 in SEQ ID NO:1; ii) a negatively charged amino acid in the region corresponding to positions 90-101 in SEQ ID NO:1; and iii) a neutral or negative amino acid at a position corresponding to N94 in SEQ ID NO:1 and/or has a negative or neutral net electric charge in the region corresponding to positions 90-101 in SEQ ID NO:1.