Patent ID: 7544770

Claim:
A multilayer film, said film comprising two or more layers of polyelectrolytes, wherein adjacent layers comprise oppositely charged polyelectrolytes, wherein a first layer polyelectrolyte comprises a composite polypeptide comprising one or more non-homopolymer surface adsorption regions covalently linked to one or more functional regions forming a single polypeptide chain, wherein the composite polypeptide and the one or more non-homopolymer surface adsorption regions have the same polarity, wherein the one or more non-homopolymer surface adsorption regions comprise one or more amino acid sequence motifs, the one or more amino acid sequence motifs consisting of 5 to 15 amino acid residues and having a magnitude of net charge per residue at neutral pH of greater than or equal to 0.4, and wherein the one or more functional regions comprise 3 to about 250 amino acid residues, and are selected from the group consisting of a cysteine-containing sequence, a sequence for extracellular matrix recognition, a phosphotyrosine binding domain, a Src homology 2 domain, a protein tyrosine phosphatase domain, an N-linked glycosylation substrate sequence, an O-linked glycosylation substrate sequence, a protease recognition site, an antimicrobial peptide sequence, and a BAG domain, and wherein the composite polypeptide is an unbranched polypeptide, is not a homopolymer, is at least 15 amino acid residues long, and has an aqueous solubility at pH 4 to 10 of greater than 50 μg/mL; wherein a second layer comprises a second layer polyelectrolyte comprising a polycationic material or a polyanionic material having a molecular weight of greater than 1,000 and at least 5 charges per molecule, and a charge opposite that of the first layer polypeptide.