Patent ID: 8440985

Claim:
A method of imaging and detecting the fluorescence emitted by chromophores fixed on a substrate, by illuminating the chromophores at an excitation wavelength and by picking up the light emitted by the chromophores in response to said illumination, said substrate being at least partially transparent at the emission wavelength of the chromophores and carrying biological probes configured to be put into contact with looked-for biological targets, the method being characterized in that it comprises placing or fastening a fluid flow device on the substrate, the fluid flow device having channels and/or chambers for introducing or circulating fluids, putting the substrate carrying the fluid flow device into contact with a sensor, while interposing a stop filter between the chromophores and the sensor to reject the excitation wavelength, the filter being transparent at the emission wavelength, using the fluid flow device to bring biological targets into contact with the probes fastened on the substrate, and illuminating the substrate at the excitation wavelength of the chromophores, and using the sensor to detect the light signals emitted by the chromophores.