Patent ID: 8105550

Claim:
A microfluidic system, the system comprising: a rigid substrate material, the rigid substrate material having a surface region, the surface region being capable of acting as a handle substrate; a deformable fluid layer coupled to the surface region; one or more well regions formed in a first portion of the deformable fluid layer, the one or more well regions being capable of holding a fluid therein; one or more channel regions formed in a second portion of the deformable fluid layer, the one or more channel regions being coupled to one or more of the well regions; an active region formed in the deformable fluid layer, the active region including the one or more well regions; a non-active region formed in the deformable fluid layer, the non-active region being formed outside of the first portion and the second portion; a first fiducial marking formed within the non-active region and disposed in a spatial manner associated with at least one of the channel regions; a second fiducial marking formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions; a control layer coupled to the fluid layer, the control layer including one or more control regions; and a third fiducial marking formed within the control layer.