Patent ID: 7033580

Claim:
A process for promoting hair follicle cell growth, comprising the step of: applying percutaneously to hair follicle cells a protein kinase C-specific inhibitor or a pharmaceutically acceptable salt thereof, in an amount effective to promote hair follicle cell growth, wherein the protein kinase C-specific inhibitor is a protein kinase inhibitor in which a ratio of its 50% protein kinase A-inhibiting constant to its 50% protein kinase C-inhibiting constant is at least 3.0, wherein protein kinase A-inhibiting activity is measured by the incubation of 10 μl of the sample to be tested for determining its activity in 250 μl of a solution comprising 5 μmols of Tris-HCI buffer (pH 6.8), 2.5 μmols of magnesium acetate, 100 μg of histone Type IIS, 0.25 nmols of c-AMP and 200 μg of an enzyme from a calf heart at 30° C. for 3 minutes and protein kinase C-inhibiting activity is measured by the incubation of 10 μl of the sample to be tested for determining its activity in 250 μl of a solution comprising 2.5 μmols of magnesium acetate, 50 μg of histone Type IIIS, 20 μg of phosphatidylserine, 0.8 μg of diolein, 25 nmols of calcium chloride, 5 μg of an enzyme from a rat brain and 5 μmols of Tris-HCI buffer (pH 7.5) at 30° C. for 3 minutes, said protein kinase C-specific inhibitor or pharmaceutically acceptable salt thereof promoting hair follicle cell growth.