Patent ID: 8329392

Claim:
A method for determining the amount of testosterone in a plasma or serum sample when taken from a human, the method comprising: (a) processing a human plasma or serum sample by one or more methods to generate a processed sample, wherein at least one method used is protein precipitation; (b) purifying testosterone in the processed sample of step (a) by high turbulence liquid chromatography (“HTLC”); (c) following step (b), further purifying testosterone in the processed sample by liquid chromatography; (d) ionizing testosterone obtained in step (c) to produce one or more testosterone ions detectable by mass spectrometry; and (e) detecting the amount of one or more testosterone ion(s) by a mass spectrometer, wherein the amount of the testosterone ion(s) is related to the amount of testosterone in the plasma or serum sample when taken from a human.