Patent ID: 7846336

Claim:
A method for regeneration of a cation exchange chromatography column after the elution of a compound of interest comprising: eluting any adsorbed polypeptides from the column with an aqueous buffered solution comprising sodium chloride at a concentration of at least 500 mM, followed by flushing the column with purified water, followed by applying a 0.5 M sodium hydroxide solution to the column, followed by flushing the column with purified water, followed by applying a solution comprising 0.5 M sodium dihydrogen phosphate and 1 M phosphoric acid to the column, followed by flushing the column with purified water, followed by applying a 0.5 M sodium hydroxide solution to the column for at least 4 hours, and followed by regenerating the cation exchange chromatography column by flushing the column with purified water.