Patent ID: 7807404

Claim:
A nucleic acid sequence encoding a mutated and truncated 6-phosphofructo-1-kinase (PFK1) fragment of a wild type fungal PFK1 enzyme, wherein the nucleic acid includes at least one mutation selected from the group consisting of an encoded amino acid substitution at an amino acid that aligns with threonine 89 of SEQ ID NO: 3, and an encoded amino acid substitution at an amino acid that aligns with glycine 95 of SEQ ID NO: 3; wherein the mutation allows the PFK1 fragment to exhibit greater enzyme activity without phosphorylation than the wild type PFK1 without phosphorylation; wherein the truncated PFK1 fragment has less than 785 amino acids and is truncated at the C-terminus relative to the wild type PFK1; and wherein the PFK1 fragment has a molecular weight of about 30 kDa to about 55 kDa as determined by SDS-PAGE.