Patent ID: 8741568

Claim:
An in vitro method of detecting human papillomavirus nucleic acid in a sample, comprising: contacting said sample with forward and reverse oligonucleotide primers, wherein said primers bind to target sites in the human papillomavirus L1 gene, or the complement thereof, under conditions suitable to promote amplification of a portion of said human papillomavirus L1 gene or complement, thereby generating an amplicon; wherein said forward primer binds to a target site having the sequence SEQ ID NO: 1, and wherein said reverse primer binds to a target site having the sequence SEQ ID NO: 2; contacting said amplicon with a set of probes, wherein said set of probes comprises (i) a probe comprising a nucleic acid having at least 90% sequence identity to SEQ ID NO: 15; (ii) a probe comprising a nucleic acid having at least 90% sequence identity to SEQ ID NO: 16; (iii) a probe comprising a nucleic acid having at at least 90% sequence identity to SEQ ID NO: 17; and (iv) a probe comprising a nucleic acid having at at least 90% sequence identity to SEQ ID NO: 18; and detecting binding of said set of probes to said amplicon.