Patent ID: 8303993

Claim:
A method of producing a biological composition consisting of: removing blood from an individual to whom the biological composition is intended to be applied; centrifuging the blood to separate the blood into portions of plasma wherein the blood contains at least one of the following: a calcium chelating agent and a heparin; obtaining thrombin from at least one portion of plasma, wherein the step of obtaining thrombin from the at least one portion of plasma comprises activation of at least a portion of the coagulation pathway; adding a fibrinogen to at least another portion of plasma; adding an additional agent to the at least another portion of plasma, wherein the additional agent has a biological activity; wherein the additional agent is selected from the group consisting of analgesic compounds, antibacterial compounds, antibiotics, antifungal compounds, anti-inflammatories, antiparasitic compounds, antiviral compounds, enzymes, enzyme inhibitors, glycoproteins, growth factors, hormones, steroids, glucocorticosteroids, immunomodulators, immunoglobulins, minerals, neuroleptics, peptides, lipoproteins, tumoricidal compounds, tumorstatic compounds, toxins, vitamins, antibacterial compounds, bacteriocidal compounds and bacteriostatic compounds; wherein the at least one portion of plasma and at least another portion of plasma are platelet rich plasma portions; combining a defined portion of the obtained thrombin with the at least another portion of plasma, wherein prior to combining the defined portion of the obtained thrombin into the at least another portion of plasma, the fibrinogen is added into the at least another portion of plasma; producing the biological composition as a result of the combining; wherein the formation time of the biological composition is dependent on the defined volume of the obtained thrombin combined with the at least another portion of plasma such that the more defined volume is added the quicker the formation time.