Patent ID: 8450471

Claim:
An article of manufacture comprising: (a) a nucleic acid encoding a first transcription activator-like (TAL) effector endonuclease monomer comprising a plurality of TAL effector repeat sequences and a FokI endonuclease domain, wherein each of said plurality of TAL effector repeat sequences comprises a repeat-variable diresidue, and wherein said first TAL effector endonuclease monomer is capable of binding to a first half-site sequence of a target DNA, and (b) a nucleic acid encoding a second TAL effector endonuclease monomer comprising a plurality of TAL effector repeat sequences and a FokI endonuclease domain, wherein each of said plurality of TAL effector repeat sequences of said second TAL effector endonuclease monomer comprises a repeat-variable diresidue, and wherein said first TAL effector endonuclease monomer is capable of binding to a second half-site sequence of said target DNA, wherein said first half-site sequence and said second half-site sequence are different and are separated by a spacer sequence, and wherein said first TAL effector endonuclease monomer and said second TAL effector endonuclease monomer are capable of forming a dimer that cleaves said target DNA within a living cell when said first TAL effector endonuclease monomer is bound to said first half-site sequence and said second TAL effector endonuclease monomer is bound to said second half-site sequence.