Patent ID: 7022475

Claim:
A method for predicting CYP3A5 expression level in a subject comprising determining the nucleotide present in each CYP3A5 allele of the genomic DNA of said subject at the location(s) selected from the group consisting of: (a) the position corresponding to nucleotide 23 of SEQ ID NO:73 within intron 3 of the Cyp3A5 gene: (b) the position corresponding to nucleotide 29 of SEQ ID NO:74 within exon 7 of the Cyp3A5 gene; and (c) the positions corresponding to both nucleotide 23 of SEQ ID NO:73 and nucleotide 29 of SEQ ID NO:74; wherein the presence of an A at the position corresponding to nucleotide 23 of SEQ ID NO:73 on at least one CYP3A5 allele of said subject predicts a relatively high level of expression of CYP3A5 as compared to the presence of a G at that position and the presence of a G at the position corresponding to nucleotide 23 of SEQ ID NO:73 on each CYP3A5 allele of said subject predicts a relatively low level of expression of CYP3A5 as compared to the presence of an A at that position; wherein the presence of a G at the position corresponding to nucleotide 29 of SEQ ID NO:74 on at least one CYP3A5 allele of said subject predicts a relatively high level of expression of CYP3A5 as compared to the presence of an A at that position and the presence of an A at the position corresponding to nucleotide 29 of SEQ ID NO:74 on each CYP3A5 allele of said subject predicts a relatively low level of expression of CYP3A5 as compared to the presence of a G at that position; and wherein the presence of an A at the position corresponding to nucleotide 23 of SEQ ID NO:73 and a G at the position corresponding to nucleotide 29 of SEQ ID NO:74 on at least one CYP3A5 allele of said subject predicts a relatively high level of expression of CYP3A5 as compared to the presence of a G at the position corresponding to nucleotide 23 of SEQ ID NO:73 and an A at the position corresponding to nucleotide 29 of SEQ ID NO:74 on at least one CYP3A5 allele of said subject and the presence of either a G at the position corresponding to nucleotide 23 of SEQ ID NO:73 or an A at the position corresponding to nucleotide 29 of SEQ ID NO:74 on each CYP3A5 allele of said subject predicts a relatively low level of expression of CYP3A5 as compared to the presence of either an A at the position corresponding to nucleotide 23 of SEQ ID NO:73 or a G at the position corresponding to nucleotide 29 of SEQ ID NO:74 on each CYP3A5 allele of said subject.