Patent ID: 8003389

Claim:
A method for introducing biologically active molecules into animal or human cells by means of electric current, comprising: i. suspending the cells and dissolving the biologically active molecules in a buffer solution which has a buffer capacity of at least 20 mmol×l −1 ×pH −1 and an ionic strength of at least 200 mmol×l −1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C. to form a suspension, wherein the buffer solution comprises a concentration of potassium ions (K + ) between 2 and 6 mmol×l −1 K + , a concentration of sodium ions (Na + ) between 100 and 150 mmol×l −1 Na + and at least 10 mmol×l −1 magnesium ions (Mg 2+ ); and ii. applying an electric voltage to the suspension so as to introduce said biologically active molecules into animal or human cells.