Patent ID: 6864057

Claim:
A method for obtaining a DNA complementary to a mRNA, the method comprising: contacting the mRNA having a polyadenosine (polyA) tail with a primer mixture, the mixture comprising a plurality of primers wherein each primer comprises at least 5 contiguous deoxythymidines at a 3′ end of the primer, and at least 2 independently selected non-deoxythymidine nucleotides positioned 3′ of the contiguous deoxythymidine nucleotides, wherein the non-deoxythymidine nucleotides comprise a sequence selected from the group consisting of VV, VTV, VTVV, VTVVV, VTVVTV, VTTV, VTTTV, VTVVV, and VVVVV and combinations thereof, wherein V is deoxyadenosine, deoxycytidine, or deoxyguanosine; and reverse transcribing the mRNA using a reverse transcriptase to produce a DNA strand complementary to the mRNA.