Patent ID: 8013122

Claim:
A method of purifying apolipoprotein A-1, comprising: a) mixing plasma fraction IV with a solution of 1-8 M urea, forming a fraction IV pretreatment solution; b) loading the pretreatment solution acquired in step a) to a first anion exchange chromatography column, and then eluting with a buffer I comprising 1-8 M urea having a conductivity of 1-4 mS/cm to obtain an eluate I that mainly contains apoA-1 protein; c) loading eluate I to a second anion exchange chromatography column, first eluting with a buffer II, having a conductivity of 1-15 mS/cm, and later eluting with a buffer III, having a conductivity of 30-100 mS/cm, to obtain pure apoA-1 protein.