Patent ID: 7867701

Claim:
A method for the amplification of nucleic acids, said method consisting of the following steps: a) a nucleic acid is chemically converted with a reagent, whereby 5-methylcytosine remains unchanged and cytosine is converted to uracil or another base similar to uracil in its base-pairing behavior, b) the segments to be amplified of the converted nucleic acid are hybridized with at least two first primer oligonucleotides, which have two domains: the sequence-specific domain found on the 3′-end hybridizes to the segment to be analyzed, while the generic domain found on the 5′-end does not hybridize, c) a first amplification reaction is conducted by means of a polymerase, d) two second primer oligonucleotides which hybridize to the generic domains of the first primer oligonucleotides and hybridize to the amplificate generated in step (c), wherein one of said two second primer oligonucleotides is labeled, and e) the sequence of the amplificate is investigated.