Patent ID: 7700325

Claim:
A method for determining a haplotype of a subject, the haplotype comprising at least three polymorphic markers that are one or more kilobase pairs apart, comprising the steps of: (a) diluting a nucleic acid sample from the subject to a single nucleic acid molecule dilution; (b) amplifying the single nucleic acid molecule dilution with at least a first, a second and a third primer pair, wherein each primer pair flanks a nucleic acid region consisting of about 100 bp, each primer pair thereby producing an amplicon consisting of about 100 bp, and wherein the at least first, second and third primer pair each are designed to amplify a different nucleic acid region designated as a first, a second and a third nucleic acid region, wherein the first, second, and third nucleic acid region each comprise at least one polymorphic marker designated as a first, a second and a third polymorphic marker, wherein the first, second and third polymorphic markers are one or more kilobase pairs apart; (c) genotyping the polymorphic marker in the first, second and third nucleic acid regions using primer extension and MALDI-TOF mass spectrometric detection thereby resulting in a first, a second and a third genotype; and (d) determining the haplotype comprising polymorphic markers one or more kilobase pairs apart from the first, second and third genotypes to obtain a haplotype for the subject.