Patent ID: 7812217

Claim:
A method for characterising recombined restorer lines of Brassica napus for Ogura cms presenting a Rfo insertion deleted of the radish Pgi-2 allele and recombined with the Pgi-2 gene from Brassica oleracea , and having an agronomic value characterised by female fertility, transmission rate of Rfo and vegetative vigour, comprising a step wherein the presence of the five markers PGIol, PGIUNT, PGIint, BolJon and CP418 is detected in said recombined restorer lines and wherein said markers comprise the following sequences: PGIol marker: SEQ ID NO:1; PGIUNT marker: SEQ ID NO:2; PGIint marker: SEQ ID NO:3; BolJon marker: SEQ ID NO:4; and CP418 marker: SEQ ID NO:5.