Patent ID: 8104330

Claim:
A method for analysis by liquid chromatography in which a sample to be analyzed is introduced into a separation column together with a mobile phase and sample components separated by and eluted from the column are detected by a detector, the method comprising the steps of: keeping the column adiabatic; setting the temperature of the mobile phase supplied through one of two mobile phase flow channels to a constant temperature higher than an upper limit of a target temperature range of the mobile phase in the column and the temperature of the mobile phase supplied through the other mobile phase flow channel to a constant temperature lower than a lower limit of the target temperature range of the mobile phase in the column; mixing two streams of the mobile phase supplied through the two mobile phase flow channels and introducing the mixed mobile phase into the column; and changing the temperature of the mobile phase in the column over time for carrying out temperature gradient elution by changing the mixing ratio between the two streams of the mobile phase over time by regulating the flow rates of the two streams of the mobile phase flowing through the two mobile phase flow channels.