Patent ID: 8551695

Claim:
A method for identifying starch binding sites of starch binding domain in CBM family, comprising: (a) obtaining structure of objective starch binding domain by NMR spectroscopy; (b) simulating binding mode of objective starch binding domain between affected residues from chemical shift perturbation of the structure of objective starch binding domain and an oligosaccharide via a docking tool to present predicted binding residues; (c) obtaining crystal structure of a complex of objective starch binding domain and the oligosaccharide via X-ray crystallography to present ligand-binding residues; and (d) selecting matched residues from the predicted binding residues and the ligand-binding residues to identify the starch binding sites of starch binding domain; wherein the CBM family is consisting of CBM20, CBM21, CBM25, CBM26, CBM34, or CBM41.