Patent ID: 7083951

Claim:
A method for multiplexed detection of PCR amplified products comprising: providing a DNA sample having up to about one-hundred unique target DNA sequences of interest for a sample multiplex PCR amplification reaction, providing necessary reagents and primers for said sample multiplex POR amplification reaction, forming a sample PCR product by conducting said sample multiplex PCR amplification reaction using said DNA sample having up to about one-hundred unique target DNA sequences of interest, a unique reporting DNA sequence complementary to a region on each target DNA sequence, wherein each reporting DNA sequence includes a fluorescence resonant energy transfer marker means, forming a microsphere mix comprising a plurality of optically encoded microspheres, each microsphere having a unique physically-detectable code, each microsphere bound to a unique biotinylated oligonucleotide, wherein each unique physically-detectable code corresponds to specific oligonucleotide having a DNA sequence complementary to each unique reporting DNA sequence, forming a sample hybridization product by adding said sample PCR product to said microsphere mix, forming a background PCR product by conducting a background multiplex PCR amplification reaction using at least one control DNA sequence, the control DNA sequence differing from each of the target DNA sequences of interest, wherein said background PCR amplification reaction is run under the same conditions and using the same primers and reagents as said sample PCR amplification reaction, forming a background hybridization product by adding said background PCR product to said microsphere mix, wherein the presence or absence of false positives is determined; and determining the existence of each of said target DNA sequence of interest specified by said reporting DNA sequence by comparing the fluorescence of said sample hybridization product with said background hybridization product using flow cytometry.