Patent ID: 8436152

Claim:
A method for purifying Apolipoprotein A-I (ApoA-1) and Alpha-1-Antitrypsin (AAT) from a single starting human plasma fraction containing both proteins comprising: i) treating a starting human plasma fraction comprising ApoA-1 and AAT to separate an ApoA-1 containing fraction from an AAT containing fraction, comprising a) treating the starting human plasma fraction which is used as the starting material such that both ApoA-1 and AAT are solubilized; b) precipitating the solubilized ApoA-1 by adding ethanol to a concentration of 8-14% v/v and adjusting the pH to about 5 to about 6 so that ApoA-1 precipitates and AAT remains in solution; and c) separating precipitated ApoA-1 from the solution containing AAT; and ii) separately purifying ApoA-1 and AAT in one or more processing steps.