Patent ID: 8741630

Claim:
A method of forming a fluidics chamber, said method comprising the steps of: a) randomly distributing a population of microspheres to an array substrate having a surface comprising a plurality of depressions, wherein said population of microspheres comprises at least a first and second subpopulation of microspheres comprising a target nucleic acid, said first subpopulation having a target nucleic acid that is different from the target nucleic acid of the second subpopulation; b) providing a population of blank microspheres and randomly distributing said population of blank microspheres to the array substrate, wherein the microspheres are present in a ratio of microspheres comprising a target nucleic acid to n 2 blank microspheres, wherein n is the number of blank microspheres separating the microspheres comprising target nucleic acid; c) applying a force to said substrate, thereby moving microspheres to the bottoms of the depressions; d) mating the substrate to a lid, said lid having at least one inlet port and one outlet port; and e) clamping said lid to said substrate a sealant being disposed between said lid and said substrate, thereby forming a fluidics chamber.