Patent ID: 7524950

Claim:
A method of forming an addressable nucleotide array, comprising providing a first nucleotide compound selected from a nucleotide, an oligonucleotide, and a polynucleotide, wherein the first nucleotide compound is dissolved in a first solution including a first co-solvent and a second co-solvent, wherein the first co-solvent comprises an organic salt, wherein the organic salt comprises a substituted heterocyclic organic cation and an anion, wherein the organic salt has a pKa of about 6 to 8, wherein the organic salt does not reduce the reaction characteristics of the first solution, wherein the first solution has a viscosity of about 5 to 20 cP and a surface tension of about 25 to 45 mN/m at a temperature of about 30 to 40° C.; disposing the first solution onto a first position on a substrate, wherein a structure is disposed in the first position, wherein the structure has a second nucleotide compound disposed thereon, wherein the second nucleotide compound is selected from a nucleotide, an oligonucleotide, and a polynucleotide; and disposing a second solution on the first position of the substrate, wherein the second solution includes an activator, wherein the activator initiates the formation of a third nucleotide compound that includes the first nucleotide compound and the second nucleotide compound.