Patent ID: 8304218

Claim:
A multi-component system for producing a peroxycarboxylic acid comprising (a) providing a first component comprising: (i) a carboxylic acid ester substrate; (ii) an enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme having a CE-7 signature motif that aligns with SEQ ID NO: 2 using CLUSTALW, said signature motif comprising: (1) an RGQ motif at amino acid positions aligning with 118-120 of SEQ ID NO:2; (2) a GXSQG motif at amino acid positions aligning with 179-183 of SEQ ID NO:2; and (3) an HE motif at amino acid positions aligning with 298-299 of SEQ ID NO:2; said enzyme comprising at least 90% amino acid identity to SEQ ID NO: 2, 14, 16, 73 or 74; and (iii) at least one cosolvent comprising an organic solvent having a log P of less than about 2, wherein log P is defined as the logarithm of the partition coefficient of a substance between octanol and water, expressed as P=[solute] octanol /[solute] water and wherein the cosolvent is not a substrate for said enzyme catalyst; wherein said first component is a substantially non-aqueous formulation of (i)-(iii); and (b) providing a second component comprising a source of peroxygen in water; wherein said first component and said second component are combined to produce an aqueous reaction formulation comprising a peroxycarboxylic acid and wherein said cosolvent solubilizes the carboxylic acid ester substrate in the aqueous reaction formulation without substantial loss of perhydrolytic activity of the enzyme catalyst.