Patent ID: 8889887

Claim:
A pentamethine cyanine fluorescent dye of formula I: wherein X is CHO or CHCR 3 R 4 ; R 1 and R 2 are independently selected from the group consisting of (CH 2 ) n R 7 , (CH 2 ) m OR 8 , (CHR 9 CH 2 O) p R 8 and CH 2 C 6 H 4 R 7 ; R 3 and R 4 are independently selected from the group consisting of CN, COOH and COOR 16 ; R 5 , R 6 and R 7 are independently selected from the group consisting of H, SO 3 R 10 and COOR 11 ; R 8 is H or C 1-18 alkyl; R 9 is H or CH 3 ; R 10 is N(R 12 R 13 R 14 R 15 ); R 11 is C 1-18 alkyl; R 12 , R 13 , R 14 , R 15 and R 16 are independently selected from the group consisting of H, C 1-18 alkyl, (CH 2 ) m OR 8 and (CHR 9 CH 2 O) p R 8 ; Y− is halogen anion or OTs−; and n, m and p are integers of 0-18.