Patent ID: 8435758

Claim:
A transgenic oleaginous Yarrowia lipolytica host cell comprising: (a) a heterotrimeric SNF1 protein kinase signaling network having reduced activity when compared to the activity of a heterotrimeric SNF1 protein kinase signaling network of a control Yarrowia lipolytica host cell, wherein said reduced activity is due to: (i) down-regulation of a polynucleotide encoding the SNF1 alpha-subunit of the heterotrimeric SNF1 protein kinase, wherein the SNF1 alpha-subunit is an amino acid sequence having at least 95% amino acid identity with SEQ ID NO:27; (ii) up-regulation of a polynucleotide encoding the regulatory domain of the SNF1 alpha-subunit, wherein the regulatory domain of the SNF1 alpha-subunit is encoded by nucleotides +835 to +1740 of SEQ ID NO:26; or (iii) up-regulation of a polynucleotide encoding a catalytically inactive SNF1 alpha-subunit having at least 95% identity with SEQ ID NO: 27; and (b) an increase in total lipid content when compared to the total lipid content of said control Yarrowia lipolytica host cell; wherein said control Yarrowia lipolytica host cell does not comprise (i), (ii), or (iii).