Patent ID: 8114404

Claim:
A composition comprising two different proteins produced by a process comprising the steps of: (a) isolating a gene encoding a full length sequence of a human V H region isolated from a B cell clone of a human patient having a B cell mediated pathology selected from the group consisting of refractory low grade lymphoma and follicular B cell lymphoma; (b) linking said gene encoding said entire V H region and a gene encoding at least a portion of an immunoglobulin constant region in an expression vector to form a gene encoding the first of said different proteins; (c) isolating a gene encoding a full length sequence of a human V L region isolated from a B cell clone of said patient having said B cell mediated pathology selected from the group consisting of refractory low grade lymphoma and follicular B cell lymphoma; (d) linking said gene encoding said entire V L region and a gene encoding at least a portion of an immunoglobulin constant region into said expression vector to form a gene encoding the second of said different proteins; (e) introducing said expression vector into insect cells, wherein said expression vector contains two separate expression cassettes each having a baculovirus promoter and a heterologous secretory signal sequence, wherein (i) said promoters are selected from the group consisting of p10 promoter and polyhedrin promoter, and (ii) said secretory signal sequences are selected from the group consisting of human placental alkaline phosphatase secretory signal sequence, honey bee melittin secretory signal sequence, and the endogenous secretory signal sequence associated with the immunoglobulin genes isolated from said patient; and (f) allowing the expression of said two different proteins having the same V H and V L amino acid sequences as said patient; wherein said composition elicits an active immune response.