Patent ID: 8624080

Claim:
A process of producing one or more than one protein of interest, comprising: (a) providing a plant or a plant cell comprising (i) in a nuclear chromosome a first heterologous nucleotide sequence comprising: a nucleotide sequence encoding an RNA replicon, and a first chemically inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase for replicating said RNA replicon and said one or more than one protein of interest; and said RNA replicon being a plus-sense single-stranded RNA replicon derived from a plant virus of genus Potexvirus or Tobamovirus; (ii) a second heterologous nucleotide sequence comprising a sequence encoding a protein enabling cell-to-cell movement of said RNA replicon, wherein said second heterologous nucleotide sequence comprises a second chemically inducible promoter operably linked to said sequence encoding said protein enabling cell-to-cell movement of said RNA replicon; and (b) inducing, in said plant or plant cell of step (a), said first and said second chemically inducible promoter, thereby producing said one or more than one protein of interest in said plant or in said plant cell, respectively.