Patent ID: 8361482

Claim:
A method for immunizing a subject against infection by Mycobacterium tuberculosis (Mtb) or for eliciting an immune response to Mtb in said subject or for preventing a recurrence of symptoms of tuberculosis in a patient with a latent Mtb, comprising the step of administering to said subject a composition comprising a recombinant Bacille Calmette-Guerin (BCG) comprising at least one DosR regulon gene that is up-regulated, and at least two nucleic acid sequences which are different from each other, each of which encode one or more genes that are overexpressed, said at least two nucleic acid sequences including i) a first nucleic acid sequence encoding at least one Mycobacterium tuberculosis (Mtb) antigen; and ii) a second nucleic acid sequence encoding at least one Mtb reactivation antigen that is not a DosR antigen.