Patent ID: 6881539

Claim:
A method for the identification and isolation of a genetic sequence from an organism, wherein disruption of genomic DNA of said organism by a transposable element flanking said genetic sequence is associated with a mutant phenotype, said method comprising the following steps: a) segregating a plurality of organisms by the presence or absence of said mutant phenotype, wherein the genomic DNA of each organism comprises at least one copy of said transposable element; b) obtaining a mutant genomic DNA sample from at least one of said organisms exhibiting said mutant phenotype and a wild-type genomic DNA sample from at least one of said organisms not exhibiting said mutant phenotype; c) fragmenting at least one of said mutant and at least one of said wild-type genomic DNA samples to produce DNA fragments; d) attaching an adapter to at least one of said mutant DNA fragments and to at least one of said wild-type DNA fragments, resulting in a collection of adapter-modified DNA fragments; e) amplifying said mutant and wild-type adapter-modified DNA fragments to yield amplification products comprising said genetic sequence wherein said amplification employs a first oligonucleotide primer which selectively hybridizes under stringent hybridization conditions to said adapter sequence, and a second oligonucleotide primer which selectively hybridizes under stringent hybridization conditions to said transposable element; and, f) isolating an amplification product present in said organism exhibiting said mutant phenotype and absent in said organism not exhibiting said mutant phenotype, wherein said isolated amplification product comprises said genetic sequence associated with said mutant phenotype.