Patent ID: 7666628

Claim:
A method for the expression of a heterologous polypeptide by a host cell said method comprising: a) introducing into a host cell a chimeric nucleic acid sequence comprising: 1) a first nucleic acid sequence capable of regulating the transcription in said host cell of 2) a second nucleic acid sequence, wherein said second sequence encodes a fusion polypeptide and comprises (i) a) a nucleic acid sequence encoding an oleosin protein having the amino acid sequence shown in SEQ ID NO:2 or SEQ ID NO:5 or b) a nucleic acid encoding a caleosin protein and having the nucleic acid sequence shown in SEQ ID NO:34 or SEQ ID NO:35 to provide targeting of the fusion polypeptide to a lipid phase linked in reading frame to (ii) a nucleic acid sequence encoding the heterologous polypeptide; and 3) a third nucleic acid sequence encoding a termination region functional in the host cell; and b) growing said host cell to produce the fusion polypeptide.