Patent ID: 7855078

Claim:
A method of flow cytometry, comprising the steps of: a. obtaining sperm cells of a mammal; b. injecting said sperm cells at an injection point along the central longitudinal axis of a fluid stream within a nozzle of a flow cytometer; c. forming a plurality of droplets in said fluid stream; d. entraining in each of a portion of said plurality of droplets one of said sperm cells injected into said fluid stream; e. analyzing said sperm cells entrained in said portion of said plurality of droplets; f. discriminating between said sperm cells to generate two populations based upon at least one sperm cell characteristic; and g. adjustably varying axial location of said injection point of said sperm cells along said central longitudinal axis in said fluid stream within said nozzle of said flow cytometer to increase resolution of said at least two populations of said sperm cells based upon said at least one sperm cell characteristic.