Patent ID: 7455981

Claim:
A method for detecting an insecticidal polypeptide in a biological sample, comprising the steps of: (a) obtaining a biological sample suspected of containing an insecticidal polypeptide; (b) contacting said sample with an antibody under conditions effective to allow the formation of immune complexes, wherein said antibody is generated by using a polypeptide comprising domains I and II of a first native B. thuringiensis δ-endotoxin Cry1A, domain III of a second native B. thuringiensis δ-endotoxin Cry1F protein, and all or a portion of a protoxin segment of a native δ-endotoxin Cry1A, Cry1F or combination Cry1F-Cry1A protein as an immunogen; and (c) detecting the immune complexes so formed.