Patent ID: 6995010

Claim:
A system for transferring a foreign gene into a cell, which contains: a. a combination of: 1. an adenoviral vector containing a first nucleic acid which has a sequence in which ITRs from adeno-associated virus are positioned on both sides of a gene of interest to be transferred; and 2. an adenoviral vector containing a second nucleic acid which has a rep gene from adeno-associated virus in which the expression of Rep52 protein and Rep40 protein are prevented by changing the 225 th amino acid residue in Rep78 from methionine to glycine and a promoter for expressing the rep gene and in which a stuffer sequence put between two recombinase recognition sequences is inserted between the rep gene and the promoter; or b. an adenoviral vector containing: a first nucleic acid which has a sequence in which ITRs from adeno-associated virus are positioned on both sides of a gene of interest to be transferred; and a second nucleic acid which has a rep gene from adeno-associated virus and a promoter for expressing the rep gene and in which a stuffer sequence put between two recombinase recognition sequences is inserted between the rep gene and the promoter.