Patent ID: 7294487

Claim:
A method of subjecting a DNA molecule to a DNA synthesis reaction, comprising the steps of: a) preparing a DNA molecule by attaching a first linker sequence at one end of the DNA molecule and attaching a second linker sequence, different from said first linker sequence, at the other end of the DNA molecule; and b) subjecting said DNA to a DNA synthesis reaction with a primer set comprising: i) a first primer set population, wherein the 5′ sequence of primers of said first primer set population is complementary to said first linker sequence and the 3′ sequence of primers of said first primer set population comprises a specificity region; and ii) a second primer set population, wherein the 5′ sequence of primers of said second primer primer set population is complementary to said second linker sequence and the 3′ sequence of primers of said second primer set population comprises a specificity region; wherein said specificity regions of said first and second primer set populations comprise random combinations of A, T, G and C.