Patent ID: 8367398

Claim:
A microfluidic apparatus comprising: a sample chamber comprising an inlet and a receiving portion, wherein a backflow prevention unit is disposed in the receiving portion; a sample separation unit which receives a sample from the sample chamber and separates a supernatant from the sample using a centrifugal force; a buffer chamber which receives a reaction buffer; a washing buffer chamber which receives a washing buffer; a reaction chamber which is connected to and receives the supernatant from the sample separation unit, is connected to and receives the reaction buffer from the buffer chamber, is connected to and receives the washing buffer from the washing buffer chamber, and is coated with capture antibodies or antigens for capturing a specimen; a supernatant metering chamber which is disposed between the sample separation unit and the reaction chamber to meter an amount of the supernatant; and a detection chamber which is connected to the reaction chamber, receives a final reaction material from the reaction chamber, and has a space in which absorbance is measured for testing a specimen of the final reaction material.