Patent ID: 8802367

Claim:
A method of detecting a nucleic acid, said method comprising in sequence: picking up a whole single-cell from a sample containing at least a single-cell having a cell membrane, lysing the cell membrane of the sampled whole single-cell and extracting nucleic acids from the cell, degrading DNA of the extracted nucleic acids with DNase, hybridizing mRNA of the total RNA contained in the whole single-cell with oligo (dT) fixed onto a carrier, said carrier consisting of particles and the total surface area of the particles is from 0.1 cm 2 to and including 10 cm 2 , a volume occupancy rate of the particles in an amplification reaction solution is 1% or less, and a total number of oligo (dT) molecules fixed onto the carrier is 10 12 or more, performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.