Patent ID: 8765685

Claim:
A method for treating a subject having edema, comprising administering a therapeutic amount of a hyaluronidase glycoprotein to the subject to treat the edema, whereby the edema is treated, wherein: the hyaluronidase glycoprotein is administered locally to the site of the edema or is administered systemically; the hyaluronidase glycoprotein: a) contains at least one sugar moiety that is covalently attached to an asparagine residue of the hyaluronidase polypeptide; b) is neutral active; and c) consists of either: i) a sequence of amino acids contained within SEQ ID NO:1 that includes amino acids 36-464 of SEQ ID NO: 1 and is truncated within the C-terminal region of SEQ ID NO:1 so that it does not comprise the full-length of the polypeptide whose sequence is set forth in SEQ ID NO:1, wherein the truncation is at a residue selected from among 477, 478, 479, 480, 481, 482 and 483 of SEQ ID NO:1; or ii) a sequence of amino acids that contains amino acid substitutions in the sequence of amino acids of i), whereby the amino-acid substituted hyaluronidase glycoprotein consists of a sequence that has at least 91% amino acid sequence identity with a sequence of amino acids of i); or iii) a sequence of amino acids that has more than 99% sequence identity with a sequence of amino acids of i); or iv) a sequence of amino acids that at least 98% sequence identity with a sequence of amino acids of i) in which the C-terminus terminates at residue 483.