Patent ID: 8394631

Claim:
A method for processing cells in a biochamber, the method comprising: a) culturing said cells with culture media for a predetermined time period; b) displacing the culture media from the biochamber into a waste container by introducing a biocompatible first rinse solution; c) replacing the first rinse solution with a cell harvest solution comprising an enzyme that allows for the detachment of cells from each other and/or from the biochamber surface; d) incubating the contents of the biochamber for a predetermined period of time to allow the detachment of cells adhered to each other and/or the biochamber surface, wherein said cells remain settled on the surface; e) displacing the cell harvest solution into a waste container by introducing a second rinse solution into the biochamber, wherein said cells remain settled on the surface; and f) agitating the biochamber to bring settled cells into suspension, wherein said method produces a mixed cell population that is at least 80% viable and at least 5% of the cells express CD90.