Patent ID: 7517668

Claim:
A process for producing a protein, comprising: (a) providing a recombinant DNA cloning vector system that integrates into the genome of an Aspergillus oryzae host in one or more copies, the vector system comprising: DNA sequences encoding functions facilitating gene expression comprising a promoter, transcription initiation sites, and transcription terminator and polyadenylation function, wherein the promoter is a fungal promoter; a DNA sequence encoding a marker that allows for selection of transformants; and a DNA sequence encoding the protein, wherein the DNA sequence encoding the protein is operatively linked to the DNA sequences encoding functions facilitating gene expression; (b) transforming the Aspergillus oryzae host, which does not harbour a functional gene for the marker, with the recombinant DNA cloning vector system of step (a); (c) culturing the transformed Aspergillus oryzae host in a suitable culture medium; and (d) recovering the protein.