Patent ID: 8205856

Claim:
A microfluidic valve comprising: a multi-level unitary elastomeric valve member comprising: a flexible membrane portion having a flexible membrane thickness; and a base membrane portion integrally formed with the flexible membrane portion, the base membrane portion surrounding the flexible membrane portion, the base membrane portion having a base membrane thickness, the base membrane thickness being greater than the flexible membrane thickness, wherein the base membrane portion and the flexible membrane portion form a single monolithic member; a first fluid access port in fluid communication with a first fluid channel and the flexible membrane portion; a second fluid access port in fluid communication with a second fluid channel and the flexible membrane portion, the second fluid channel being separate from the first fluid channel, wherein the flexible membrane portion is adapted to deflect away from the first and second fluid access ports to allow fluid to pass between the first and second fluid access ports; and a pressure port in fluid communication with the flexible membrane portion, wherein the flexible membrane portion is adapted to form a seal with the first and second fluid access ports upon application of closing pressure.