Patent ID: 8057659

Claim:
A method for detecting glucose in a sample comprising the steps of: (a) applying a sample to be evaluated for the presence of glucose to a test strip, wherein the test strip comprises: a support portion defining a sample chamber; a working and a counter electrode disposed within the sample chamber; and a reagent composition disposed in the sample chamber, said reagent composition comprising (i) an active redox enzyme effective to oxidize glucose in the sample, (ii) a mediator compound, and (iii) a shuttle compound, wherein the shuttle compound and the mediator compound are different from one another; wherein when a sample containing analyte is introduced into the sample chamber, the redox enzyme oxidizes glucose the analyte and produces an inactive enzyme that is reduced, the mediator is [Os(Mebpy) 2 Pic] +/2+ and the shuttle is ferri/ferrocyanide, and (b) applying a potential between the working and the counter electrodes to generate current that is indicative of the amount of glucose in the sample.