Patent ID: 8394340

Claim:
A microfluidic test module for detecting target nucleic acid sequences in a fluid, the test module comprising: an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences; electrode pairs for receiving an electrical pulse; electrochemiluminescent (ECL) probe spots in contact with each of the electrode pairs respectively, the ECL probe spots containing ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores; an array of hybridization chambers containing the ECL probes for different target nucleic acid sequences; a plurality of calibration sources each configured to provide a calibration emission, the calibration sources being distributed throughout the array of hybridization chambers such that each of the calibration sources are surrounded by a three-by-three square of the hybridization chambers; wherein, the mass of the ECL probes in each of the probe spots is less than 270 picograms.