Patent ID: 7572907

Claim:
A method of forming polynucleotides, comprising: providing a structure X selected from where R1 is selected from oxycarbonate, aryl ester, alkyl ester, alkyl silane, aryl silane, alkylsiloxane, arylsiloxane, alkylarylsilane, and alkylarylsiloxane and functional groups, where the functional group is selected from carbonate, ester, amide, carbamate, silane, siloxane, orthoester, acetal, and ketal; where R2 is selected from benzyl, alkylbenzyl, dialkylbenzyl, trialkylbenzyl, thioalkylbenzyl, phenylthiobenzyl, dithioalkylbenzyl, trithioalkylbenzyl, thioalkylhalobenzyl, alkyloxybenzyl, dialkyloxybenzyl, halobenzyl, dihalobenzyl, trihalobenzyl, and esterified salicyl; where B is optionally a purine or pyrimidine base or analog thereof; wherein n is from 1 to 350; and wherein ● is a substrate; and oxidizing and deprotecting structure X simultaneously in an aqueous buffer solution, wherein the aqueous buffer solution comprises an oxidant and a nucleophile at a pH of about 6to 10.