Patent ID: 7351813

Claim:
A nucleic acid expression cassette capable of expressing human Factor IX, wherein the cassette is predominantly expressed in the mammalian liver, said cassette comprising: (a) a hepatic locus control element consisting of SEQ ID NO:4 or SEQ ID NO:9; (b) a heterologous hepatic promoter located 3′ to the hepatic locus control element, said promoter consisting of SEQ ID NO: 5; (c) a Factor IX coding sequence located 3′ to the hepatic promoter, said coding sequence comprising SEQ ID NO:2; (d) a polyadenylation signal located 3′ to the intron sequence, said polyadenylation signal consisting of SEQ ID NO:6; and (e) an intron located 3′ to the hepatic promoter and 5′ to the polyadenylation signal, wherein said intron consists of SEQ ID NO:1, wherein elements (a), (b), (c), (d) and (e) are operably linked to express the polypeptide encoded by the coding sequence.