Patent ID: 7759099

Claim:
A method for seeding cells on a luminal surface of an implantable medical device, the method comprising: providing an implantable medical device having a lumen and a luminal surface, and wherein the lumen defines a longitudinal axis; centrifuging blood to obtain a blood centrifugation product comprising blood plasma; applying a preparation media comprising calcium to the luminal surface of the implantable medical device; subsequent to applying the preparation media, applying the blood centrifugation product to the luminal surface of the implantable medical device; subsequent to applying the blood centrifugation product, introducing cells into the lumen of the implantable medical device; and axially centrifuging the implantable medical device by rotating the implantable medical device substantially around the longitudinal axis at a rate sufficient to generate between 1 g and 1000 g of centripetal acceleration on the cells, thereby seeding the cells on the luminal surface of the implantable medical device.