Patent ID: 8481266

Claim:
A DNA sequencing method, which includes the following steps: A. Said DNA is processed into multiple DNA fragments, and then constructed into multiple DNA tags; B. Amplification of every single DNA tag, and then processed into single stranded DNA; C. Utilizing a sequencing anchor which is operable to hybridize to the DNA tags and contains at least one-degenerate base to sequence every single DNA tag and thus produce sequencing signal; and D. Obtaining sequences of every single DNA tag by sequencing signal, wherein step A includes: A1. Ligating a first adaptor sequence to both ends of the DNA fragments; A2. Cutting the ligated product with a restriction enzyme to generate a free 3′ end and a free 5′ end for each of the DNA fragments; A3. Ligating a second adaptor sequence to the free 3′ and 5′ ends to produce DNA tags of equal size, the DNA tags each including the first adaptor sequence and the second adaptor sequence; said first adaptor sequence including a sequence complementary to the sequencing anchor, sequences of amplification primers, and a Type II restriction enzyme site used to process the DNA fragments into DNA tags of equal size; said amplification primers being configured for PCR; and said second adaptor sequence including an anchor sequence for normalization of sequence signals.