Patent ID: 7192735

Claim:
A method for phosphopantetheinylating a substrate in a cell, comprising transforming the cell with a first nucleic acid molecule comprising an expressible form of a nucleotide sequence encoding a heterologous Bacillus phosphopantetheinyl transferase, and a second nucleic acid molecule comprising an expressible form of a nucleotide sequence encoding a heterologous protein which is a substrate of the phosphopantetheinyl transferase, such that phosphopantetheinylation of the substrate in the cell occurs, wherein the heterologous Bacillus phosphopantetheinyl transferase comprises: (a) an amino acid sequence of an enzyme selected from the group consisting of Sfi. Psf-1, Gsp and Lpa-14; or (b) an amino acid sequence at least 90% identical to the amino acid sequence of an enzyme selected from the group consisting of Sfp, Psf-1, Gsp and Lpa-14 and comprising the amino acid sequences set forth in SEQ ID NO: 57 and SEQ ID NO:59, and wherein the substrate comprises the amino acid sequence shown in SEQ ID NOs: 70 or 71, and is capable of being phosphopantetheinylated by an enzyme selected from (a) or (b).