Patent ID: 8685739

Claim:
A method of diagnosing a disease state in a subject, wherein the disease state is caused or effected by a pneumococcal infection, comprising: (a) obtaining a plurality of diseased biological test samples known to have the disease state, wherein the biological test samples include metabolites selected from the group consisting of citrate, trigonelline, acetylcarnitine, acetone, myo-inositol, 3-hydroxybutyrate, glucose and carnitine; (b) for each diseased biological test sample, discretely identifying more than one metabolite from said metabolites in the biological test sample and determining a respective concentration of each of the discretely identified metabolites; (c) generating a predetermined profile of the disease state using at least the respective concentration of the identified metabolites, the predetermined profile comprising a plurality of data sets; (d) obtaining a biological test sample from the subject, discretely identifying said more than one metabolite in the biological test sample, determining a respective concentration of each of the identified metabolites in the biological test sample, and determining a test profile using at least the respective concentration of the identified metabolites in the biological test sample; and (e) using multivariate statistical analysis, comparing the test profile of the biological test sample from the subject with the predetermined profile of the disease state to determine a probability of the biological test sample having the disease state.