Patent ID: 7846698

Claim:
A method for producing L-lysine comprising (a) cultivating a coryneform bacterium in an appropriate medium, wherein said bacterium harbors (1) aspartokinase prepared from a coryneform bacterium which is substantially desensitized to feedback inhibition by L-lysine and L-threonine, wherein the 279 th amino acid residue in said aspartokinase α-subunit is substituted with an amino acid other than alanine or an acidic amino acid, and wherein the 30 th amino acid residue in said aspartokinase β-subunit is substituted with an amino acid other than alanine or an acidic amino acid, wherein the residue number is counted from the N-terminal of said aspartokinase, (2) a DNA sequence encoding dihydrodipicolinate reductase prepared from coryneform bacterium, wherein said DNA sequence expresses enhanced amounts of said dihydrodipicolinate reductase asccompared to a wild-type coryneform bacterium, and (3) a DNA sequence encoding dihydrodipicolinate synthase, and (b) collecting L-lysine from the culture.