Patent ID: 8741638

Claim:
A method of simultaneously maximizing doubling rate, harvest density, and the total number of population doublings for postpartum cells in a roller bottle culture system comprising: using a rotational speed from 0.65-0.9 rpm; using at least 300 ml of growth medium in an 850 square centimeter culture bottle; using a seeding density of less than 2,500 cells per square centimeter; and incubating for between 5.5 to 6.5 days, wherein the postpartum cells that are cultured are characterized as having an identical marker profile as starting cells with respect to each of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, HLA-ABC, CD31, CD34, CD45, CD117, CD141, and HLA-DRDPDQ, and wherein the postpartum cells are positive for expression of CD10, CD13, CD44, CD73, CD90, PDGFr-alpha, and HLA-ABC, and negative for expression of CD31, CD34, CD45, CD117, CD141, and HLA-DRDPDQ.