Patent ID: 8034607

Claim:
A method of enhancing secretion of heterologous protein of interest in a yeast cell comprising the steps of: a. engineering a first yeast cell to overexpress at least two genes selected from the group consisting of S. cerevisiae CCW12, CWP2, SED1, RPPO and ER01 , K. lactis XP — 455980.1 (CCW12), C. albicans CCW12 , P. stipitis CCW12 , K. lactis ER01 , P. stipitis ERO1 , S. pombe ERO12 and ERO11 , Mus musculus ERO1LB, Homo sapien ERO1LB, K. lactis XP — 451800.1 (RPPO), P. stipitis RPPO, C. albicans XP — 888730.1 (RPPO), S. pombe RPPO, Mus musculus ARBP, and Homo sapien RPLPO, wherein the two genes encode two functionally different proteins, and wherein said heterologous protein of interest differs in structure and function from the proteins engineered to overexpress in said first yeast cell; and b. supplying the yeast cell with a nucleic acid encoding said heterologous protein of interest, wherein the secretion of said heterologous protein of interest is increased by the overexpression of said at least two genes in the first yeast cell in step a relative to the secretion of said heterologous protein in a second yeast cell that does not overexpress said at least two genes selected from the group consisting of S. cerevisiae CCW12, CWP2, SED1, RPPO and ER01 , K. lactis XP — 455980.1 (CCW12), C. albicans CCW12 , P. stipitis CCW12 , K. lactis ER01 , P. stipitis ERO1 , S. pombe ERO12 and ERO11 , Mus musculus ERO1LB, Homo sapien ERO1LB, K. lactis XP — 451800.1 (RPPO), P. stipitis RPPO, C. albicans XP — 888730.1 (RPPO), S. pombe RPPO, Mus musculus ARBP, and Homo sapien RPLPO.