Patent ID: 7691810

Claim:
An isolated, recombinant antithrombin III in which an amino acid sequence of the recombinant antithrombin III consists of the amino acid sequence at positions from 33 to 464 of SEQ ID NO:4; wherein the recombinant antithrombin III comprises four complex type N-glycoside-linked carbohydrate chains each at asparagines residues at positions 128, 167, 187 and 224 in the amino acid sequence of SEQ ID NO:4, wherein each of said carbohydrate chains lacks fucose bound to N-acetylglucosamine in the reducing end of said carbohydrate chains, wherein the recombinant antithrombin III is expressed in a CHO cell into which a DNA encoding the amino acid sequence at positions from 33 to 464 of SEQ ID NO:4 is introduced, wherein the genome of the CHO cell is modified so as to have deleted activity of an enzyme relating to synthesis of an intracellular carbohydrate nucleotide, GDP-fucose, or an enzyme relating to the modification of a carbohydrate chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in a complex type N-glycoside-linked carbohydrate chain.