Patent ID: 8093020

Claim:
A method of discriminating two or more different alleles, said method comprising: performing one or more amplification reactions comprising reagents to amplify two or more different alleles from a single sample; collecting data comprising sequential amplification signal measurements representing the degree of amplification of each allele in the amplification reaction, and the time point in the amplification reaction at which the amplification signal is measured, for a multiplicity of time points in the amplification reaction(s); determining an efficiency related transform of said data where said efficiency related transform provides an amplitude measure that is related to the efficiency of amplification in said reaction; determining an efficiency related value for each allele that is the maximum magnitude of the efficiency related transform determined for that allele; and outputting to a display, printer, or storage medium the efficiency related values for each allele, where the relative amplitudes of the efficiency related values for each allele is an indicator of the presence of each of said allele in said sample.