Patent ID: 7393633

Claim:
A method for diagnosis of the genotype of Human Papillomavirus (HPV) infection comprising: (a) amplifying DNA obtained from clinical samples to obtain biotin-containing amplified DNA using a kit comprising: (i) a DNA chip comprising a combination of each of the genotype specific HPV nucleic acid sequence probes set forth as: SEQ ID NO: 1 or the fully complementary sequence thereof, specific for HPV genotype HPV-16; SEQ ID NO: 2 or the fully complementary sequence thereof, specific for HPV genotype HPV-18; SEQ ID NO: 3 or the fully complementary sequence thereof, specific for HPV genotype HPV-31; SEQ ID NO: 4 or the fully complementary sequence thereof, specific for HPV genotype HPV-33; SEQ ID NO: 5 or the fully complementary sequence thereof, specific for HPV genotype HPV-35; SEQ ID NO: 6 or the fully complementary sequence thereof, specific for HPV genotype HPV-39; SEQ ID NO: 7 or the fully complementary sequence thereof, specific for HPV genotype HPV-45; SEQ ID NO: 8 or the fully complementary sequence thereof, specific for HPV genotype HPV-51; SEQ ID NO: 9 or the fully complementary sequence thereof, specific for HPV genotype HPV-52; SEQ ID NO: 10 or the fully complementary sequence thereof, specific for HPV genotype HPV-56; SEQ ID NO: 11 or the fully complementary sequence thereof, specific for HPV genotype HPV-58; SEQ ID NO: 12 or the fully complementary sequence thereof, specific for HPV genotype HPV-59; SEQ ID NO: 13 or the fully complementary sequence thereof, specific for HPV genotype HPV-66; SEQ ID NO: 14 or the fully complementary sequence thereof, specific for HPV genotype HPV-6; SEQ ID NO: 15 or the fully complementary sequence thereof, specific for HPV genotype HPV-11; SEQ ID NO: 16 or the fully complementary sequence thereof, specific for HPV genotype HPV-34; SEQ ID NO: 17 or the fully complementary sequence thereof, specific for HPV genotype HPV-40; SEQ ID NO: 18 or the fully complementary sequence thereof, specific for HPV genotype HPV-42; and SEQ ID NO: 19 or the fully complementary sequence thereof, specific for HPV genotype HPV-44, and a glass slide to which the probes are attached; (ii) biotin-labeled primers for amplifying DNA obtained from clinical samples; and (iii) means for labeling amplified DNA that hybridizes with the probes of the DNA chip; (b) applying the amplified DNA to the DNA chip under conditions which allow hybridization of the amplified DNA to the probes; (c) applying a biotin-binding label to the hybridized DNA on the chip; and (d) detecting hybridized DNA on the surface of the DNA chip at a position corresponding to the location of a genotype specific HPV probe by detecting the biotin-binding label, wherein detection of the biotin-binding label indicates the presence of HPV DNA in the sample which corresponds to the genotype of the HPV probe to which the DNA is hybridized.