Patent ID: 8790885

Claim:
A process for producing a coagulogen raw material used for detecting a predetermined physiologically active substance of biological origin or measuring a concentration of the physiologically active substance, comprising: heat-treating limulus amoebocyte lysate (LAL) at a temperature between 60° C. and 80° C. for a period of 10 minutes to 8 hours to produce the coagulogen raw material, wherein the LAL is supplied as a solution and comprises at least one enzyme selected from the group consisting of factor C, factor B, factor G, and precursor of clotting enzyme, and wherein said heat-treating inactivates at least a part of the enzyme in the LAL while maintaining activity of the coagulogen; whereby when an enzyme cascade is generated by the physiologically active substance activating the enzyme, the coagulogen in the coagulogen raw material is hydrolyzed into coagulin by the enzyme cascade.