Patent ID: 8779112

Claim:
An isolated modified polynucleotide encoding a modified full-length protease, wherein said modified full-length protease is a B. clausii or B. lentus alkaline serine protease, wherein one portion of said modified polynucleotide encodes a pro region of said full-length protease comprising a mutation encoding an amino acid substitution at a position equivalent to amino acid position E57 of SEQ ID NO:5, SEQ ID NO:13 or SEQ ID NO:244, wherein said substitution is chosen from the group consisting of: E57F, E57W, E57K, E57R, E57M, E57C, E57Q, E57S, E57H, E57N and E57G, and wherein another portion of said modified polynucleotide encodes a protease having at least 97% amino acid sequence identity with the amino acid sequence of SEQ ID NO:8, SEQ ID NO:16 or SEQ ID NO:247.