Patent ID: 7959801

Claim:
A device for generating micro- and nanoflow mobile phase gradients for high performance liquid chromatography, comprising: a generator of micro- and nanoflows of at least two mobile phases in different percentage compositions; an n-position gradient distributor having an inlet port, an outlet port and n couples of ports for a like number of capillary loops, each of the capillary loops being selectively connectable at a first end with the inlet port and at a second end to the outlet port, the second end being connectable to an analytical circuit comprising a chromatographic column and a detector; a micro- and nanoflow meter connected to said analytical circuit; a flow deviator having its inlet connected to the outlet of said gradient distributor, a first outlet connected to discharge and a second outlet connected to said micro- and nanoflow meter; and a control unit comprising, means of control of the generator of micro- and nanoflows such to create the different mobile phase mixture compositions, gradient generating means for controlling said gradient distributor, said gradient generating means acting on implementation and residence time of the different mobile phase mixture compositions, means for controlling a position of said flow deviator, and means for controlling a throughput of said generator of micro- and nanoflows according to information given by said micro- and nanoflow meter.