Patent ID: 6958236

Claim:
A receptor cassette encoding a chimeric receptor polypeptide comprising: 1) a DNA binding (C) domain; 2) a hinge (D) domain of an ecdysone receptor (EcR) of an insect selected from the group consisting of Manduca sexta, Chironomus tentans , and Locusta migratoria; 3) a ligand binding (E) domain that is heterologous with respect to said hinge (D) domain; and 4) an activation domain; wherein a) said DNA binding (C) domain is a Manduca sexta EcR DNA binding (C) domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is a Drosophila melanogaster EcR ligand binding (E) domain; b) said DNA binding (C) domain is a Manduca sexta EcR DNA binding (C) domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is an Agrotis ipsilon EcR ligand binding (E) domain; c) said DNA binding (C) domain is a GAL4 DNA binding domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is an Agrotis ipsilon EcR ligand binding (E) domain; d) said DNA binding (C) domain is a Manduca sexta EcR DNA binding (C) domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is an Ostrinia nubilalis EcR ligand binding (E) domain; e) said DNA binding (C) domain is a GAL4 DNA binding domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is an Ostrinia nubilalis EcR ligand binding (E) domain; f) said DNA binding (C) domain is a Manduca sexta EcR DNA binding (C) domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is a Spodopterda frugipera EcR ligand binding (E) domain; g) said DNA binding (C) domain is a GAL4 DNA binding domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is a Spodopterda frugipera EcR ligand binding (E) domain; h) said DNA binding (C) domain is a Locusta migratoria EcR DNA binding (C) domain, said hinge (D) domain is a Locusta migratoria EcR hinge (D) domain, and said ligand binding (E) domain is a Manduca sexta EcR ligand binding (E) domain; i) said DNA binding (C) domain is a Manduca sexta EcR DNA binding (C) domain, said hinge (D) domain is a Manduca sexta EcR hinge (D) domain, and said ligand binding (E) domain is a Locusta migratoria EcR ligand binding (E) domain; j) said DNA binding (C) domain is a Chironomus tentans EcR DNA binding (C) domain, said hinge (D) domain is a Chironomus tentans EcR hinge (D) domain, and said ligand binding (E) domain is a Manduca sexta EcR ligand binding (E) domain; or k) said DNA binding (C) domain is a Manduca sexta EcR DNA binding (C) domain, said hinge (D) domain is a Chironomus tentans EcR hinge (D) domain, and said ligand binding (E) domain is a Chironomus tentans EcR ligand binding (E) domain.