Patent ID: 7026115

Claim:
A kit for the fragmentation of target DNAs by one or more restriction endonucleases into fragments, and for the amplification and analysis of a subset of these fragments, which comprises: the restriction endonuclease or endonucleases; at least one double-stranded synthetic oligonucleotide adaptor having one end which can be ligated to one or both ends of the restriction fragments of the target DNA; at least one primer for the amplification of a subset of the restriction fragments of the target DNA, having 10 to 50 nucleotides and having the following structure: 5′-constant nucleotide sequence complementary to at least part of the adaptor—constant nucleotide sequence complementary to at least part of the restriction site of said restriction endonuclease—selective nucleotide sequence—3′ wherein said selective nucleotide sequence consists of one to 10 nucleotide residues, may be random or specific, and allows for the selection of a subset of DNA fragments for amplification from said target.