Patent ID: 7326533

Claim:
A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample, comprising the steps of: providing a biological sample containing nucleic acid from M. tuberculosis comprising a rpoB sequence; amplifying the rpoB sequence in an in vitro nucleic acid amplification reaction mixture comprising at least one polymerase activity, and a first primer consisting of SEQ ID NO: 2 and a second primer consisting of SEQ ID NO: 8 to produce amplified nucleic acid containing a rpoB sequence; optionally fragmenting the amplified nucleic acid; hybridizing the amplified nucleic acid to at least one detection probe consisting of SEQ ID NO:4 that hybridizes specifically to M. tuberculosis sequences; and detecting the amplified nucleic acid hybridized to at least one detection probe by detecting a label associated with the amplified nucleic acid, thereby detecting the rpoB sequences of Mycobacterium tuberculosis present in the biological sample.