Patent ID: 8418929

Claim:
A temperature controlling apparatus comprising one or a plurality of sets of nozzles which can suck and discharge a liquid through a tip, and can retain the sucked liquid, a suction and discharge mechanism which can suck and discharge a gas via the each nozzle, a temperature regulator which can maintain one or two or more set predetermined temperatures for a predetermined time regarding one or two or more predetermined temperature controlling regions provided in the each nozzle, a movement mechanism which allows relative movement between one or two or more containers which can accommodate a liquid and the nozzle, and a controlling part which instructs the movement mechanism, the suction and discharge mechanism or the temperature regulator to adjust a liquid to be sucked into the nozzle and a liquid amount thereof, a position of the sucked liquid in the nozzle, and temperature controlling of the liquid; wherein the nozzle is provided movably relative to a container capable of accommodating a liquid to be temperature-controlled, and a container capable of accommodating a sealing liquid for sealing the liquid in the nozzle by holding it from upper and lower directions in the nozzle by the movement mechanism; wherein a nozzle choking member which can choke the nozzle to prevent flow-out of a fluid from the nozzle is provided below a temperature controlling region of the nozzle or the outside of the nozzle, and further includes an on-off mechanism which opens and closes the nozzle below the temperature controlling region using the choking member; wherein the nozzle includes a mounting nozzle and a disposable tip including a mounting opening detachably mounted to the mounting nozzle and a mouth part which can suck and discharge a liquid at a tip; and wherein the disposable tip includes a large-diameter tube, a small-diameter tube formed to be thinner than the large-diameter tube, and a transition part formed between the large-diameter tube and the small-diameter tube, and the temperature controlling region is set in the small-diameter tube.