Patent ID: 8440397

Claim:
A method of identifying a nucleotide at a detection position of a target nucleic acid, wherein said target nucleic acid comprises a plurality of detection positions, said method comprising: (a) providing a plurality of concatemers immobilized on a surface, wherein said surface comprises a plurality of discrete regions and said plurality of concatemers are randomly disposed on said discrete regions such that at least 70 percent of said concatemers are individually resolvable and such that at least 70 percent of said concatemers have a nearest neighbor distance of 200 nm or greater, wherein each concatemer comprises multiple copies of a monomer and each monomer comprises: (i) a first target domain of said target nucleic acid comprising a first set of target detection positions; (ii) a first adaptor; (iii) a second target domain of said target nucleic acid comprising a second set of target detection positions; and (iv) a second interspersed adaptor, wherein said second interspersed adaptor is located between said first target domain and said second target domain, and wherein said first target domain and said second target domain are contiguous segments of said target nucleic acid; (b) detecting a nucleotide in said first set or said second set of detection positions, thereby identifying said nucleotide.