Patent ID: 8846876

Claim:
A method for derivatizing an antibody or fragment thereof comprising at least one amino acid of Formula (I), the method comprising contacting the antibody or fragment with a reagent of Formula (XIX), wherein Formula (I) corresponds to: wherein: A is phenylene; B is a bond; J is R is alkyl; each R′ is independently H, alkyl, or substituted alkyl; R 1 is H, an amino protecting group, or at least one amino acid; R 2 is OH, an ester protecting group, or at least one amino acid; and R 3 and R 4 are H; wherein Formula (XIX) corresponds to: wherein: X is a drug or a detectable label, wherein the detectable label is selected from a fluorescent, phosphorescent, chemiluminescent, chelating, intercalating, radioactive, and chromophoric moiety, and combinations thereof, or a polyalkylene glycol; L is optional, and when present is a linker selected from the group consisting of alkylene, substituted alkylene, alkenylene, substituted alkenylene, —O—, —O-(alkylene or substituted alkylene)-, —S—, —S-(alkylene or substituted alkylene)-, —S(O) k — where k is 1, 2, or 3, —S(O) k (alkylene or substituted alkylene)-, —C(O)—, —C(O)-(alkylene or substituted alkylene)-, —C(S)—, —C(S)-(alkylene or substituted alkylene)-, —N(R′)—, —NR′-(alkylene or substituted alkylene)-, —C(O)N(R′)—, —CON(R′)-(alkylene or substituted alkylene)-, -(alkylene or substituted alkylene)NR′C(O)O-(alkylene or substituted alkylene)-, —O—CON(R′)-(alkylene or substituted alkylene)-, —CSN(R′)—, —CSN(R′)-(alkylene or substituted alkylene)-, —N(R)CO-(alkylene or substituted alkylene)-, —N(R′)C(O)O—, N(R′)C(O)O-(alkylene or substituted alkylene)-, —S(O) k N(R′)—, —N(R′)C(O)N(R′)—, —N(R′)C(O)N(R′)-(alkylene or substituted alkylene)-, —N(R′)C(S)N(R′)—, —N(R′)S(O) k N(R′)—, —N(R′)—N═, —C(R′)═N—, —C(R′)═N—N(R)—, —C(R′)═N—N═, —C(R′) 2 —N═N—, and —C(R′) 2 —N(R′)—N(R′)—, where each R′ is independently H, alkyl, or substituted alkyl; L 1 is optional, and when present, is —C(R′) p —NR′—C(O)O-(alkylene or substituted alkylene)- where p is 0, 1, or 2; W is —ON(R 1 ) 2 , where each R 1 is independently H or an amino protecting group; and n is 1.