Patent ID: 7345218

Claim:
A method for producing a transgenic cotton plant comprising: (a) preparing explants from fibrous roots of cotton seedlings cultured in medium comprising about 0.05 mg/l to 0.2 mg/l of multi-effect triazole (MET); (b) culturing said root explants in medium comprising a plant hormone selected from the group consisting of (I) (2,4-dichlorophenoxy)acetic acid (2,4-D), (ii) 6-γγ-dimethylallyl(amino) purine (2iP), (iii) a mixture of 2,4-D and kinetin and (iv) a mixture of 2iP and α naphthalene acetic acid to induce callus formation; (c) transforming said callus with Agrobacterium tumifaciens comprising a first DNA encoding a chimeric gene of interest to effect the stable transfer of said chimeric gene to the genome of cells comprising the callus tissue; (d) culturing said transformed callus to induce somatic embryos and development of plantlets from said somatic embryos; and (e) rooting said plantlets to produce transgenic cotton plants having said gene of interest.