Patent ID: 8609389

Claim:
An isolated improved type protease which comprises an amino acid sequence that is at least 90% identical to SEQ ID NO: 3 or an isolated improved type protease which comprises a protein produced from an isolated DNA having at least go % sequence identity with SEQ ID NO: 42, wherein SEQ ID NO: 42 is the isolated DNA encoding the protein of SEQ ID NO: 43, and wherein said improved type protease has at least one mutation selected from the group consisting of: (A) replacement of glutamine corresponding to glutamine at position 265 in SEQ ID NO: 3 or SEQ ID NO: 43 with an acidic amino acid; and (B) replacement of glutamine at position 266 in SEQ ID NO: 3 or SEQ ID NO: 43 with an acidic amino acid, and wherein said improved type protease has milk-clotting activity.