Patent ID: 8906624

Claim:
A PCR apparatus comprising: a PCR module which comprises at least one radially distributed structure, wherein each structure comprises a PCR chip where PCR is performed; a rotating means including a shaft that penetrates a center of the PCR module, such that the PCR chip is rotated in accordance with rotation of the shaft; and a temperature zone forming means spaced apart from the PCR chip, capable of applying thermal energy to the PCR chip and allowing the rotating PCR chip to pass through different temperature zones, wherein the shaft of the rotating means is disposed to correspond to a center of the temperature zone forming means, wherein the PCR chip is formed as a single chip that comprises: a PCR unit where PCR occurs; and a pretreatment unit where a target substance of PCR is separated, and wherein the pretreatment unit comprises: three chambers, each containing a different one of three aqueous solutions including a sample solution, a washing buffer and an elution buffer, and a capturing means being connected to each of the three chambers, the three chambers including a sample chamber storing the sample solution, a washing buffer chamber storing the washing buffer and an elution buffer chamber storing the elution buffer; and fluid channels, each of which is connected to a corresponding one of the three chambers, wherein: each fluid channel is hydrophobically treated and has a different cross sectional area, wherein a respective driving force to drive each of the aqueous solutions to flow through a corresponding one of the fluid channels, is determined by a cross sectional area of each fluid channel, such that a hydrophobically-treated fluid channel with a greater cross sectional area requires a smaller driving force; said respective driving force is generated by a centrifugal force which corresponds to a rotational speed of the PCR chip, such that, when the rotational speed reaches a respective minimum value to generate a corresponding one of said respective driving force for a corresponding one of the fluid channels, a corresponding one of the three aqueous solutions flows to the capturing means via said corresponding one of the fluid channels with a corresponding cross sectional area.