Patent ID: 8679744

Claim:
Method for isolating and cleaning nucleic acids from a lysate, comprising a) lysing a biological sample containing nucleic acid with a lysis buffer; b) optionally neutralizing the preparation resulting from the lysis with a neutralisation buffer; c) mixing a reaction mixture so obtained with one or more compounds of formula (I): Y + R 1 R 2 R 3 R 4 X − (I) where Y is nitrogen or phosphorus R 1 , R 2 , R 3 and R 4 are independently from one another an unbranched or branched C 1 -C 20 -alkyl residue, C 3 -C 6 -alkenyl residue, C 3 -C 6 -alkinyl residue and/or a C 6 -C 20 -aryl residue as well as a C 6 -C 26 -aralkyl residue, and X— is an anion of an inorganic or organic single or multi-basic acid or their aqueous solution, and brought into contact with a mineral matrix, wherein the nucleic acids bind to the mineral matrix in the presence of the one or more compounds of formula I, d) washing nucleic acids with a wash buffer; and e) isolating nucleic acids, and wherein the compound of formula (I) promotes binding to the matrix.