Patent ID: 8563305

Claim:
A method for producing an antibody molecule that binds an antigen, the method comprising: (a) obtaining a sample of cells enriched for PBMC from a biological sample comprising antibody producing cells obtained from a mammal after exposure of the mammal to the antigen; (b) isolating a population of CD19 high CD3 neg CD20 low to neg CD38 high CD27 high cells from the sample of cells enriched for PBMC based on expression of each of said markers, (c) isolating from a selected CD19 high CD3 neg CD20 low to neg CD38 high CD27 high cell or progeny of the selected CD19 high CD3 neg CD20 low to neg CD38 high CD27 high cell, a nucleic acid molecule encoding at least a portion of an antibody light chain expressed by the CD19 high CD3 neg CD20 low to neg CD38 high CD27 high cell, wherein the portion of the antibody light chain comprises the variable domain, and a nucleic acid molecule encoding at least a portion of an antibody heavy chain expressed by the cell, wherein the portion of the antibody heavy chain comprises the variable domain, wherein the nucleic acid molecule that encodes the antibody light chain does not encode the antibody heavy chain; (d) transforming a recombinant cell with the nucleic acid sequence encoding at least a portion of the antibody light chain comprising the light chain variable domain and a nucleic acid sequence encoding at least a portion of the antibody heavy chain comprising the heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable were paired in the selected cell; and (e) culturing the recombinant cell to produce an antibody molecule the binds the antigen.