Patent ID: 7491539

Claim:
A method of promoting sequence specific recombination of a first DNA and a second DNA in a eukaryotic cell comprising: (a) providing the eukaryotic cell wherein the eukaryotic cell comprises the first DNA comprising a first recombination sequence selected from the group consisting of an attB sequence (SEQ ID NO:13) and an attP sequence (SEQ ID NO:14); (b) introducing into the cell the second DNA comprising a second recombination sequence selected from the group consisting of the attB sequence (SEQ ID NO:13) and the attP sequence (SEQ ID NO:14), wherein if said first DNA comprises the attB sequence, said second DNA comprises the attB sequence, or wherein if said first DNA comprises the attP sequence, said second DNA comprises the attP sequence; and (c) providing to the cell a bacteriophage lambda integrase Int selected from the group consisting of Int-h and Int-h.218 under conditions that support integrase-mediated sequence specific recombination between the first recombination sequence and the second recombination sequence, wherein if the cell comprises two attB sequences, the Int is Int-h.218, and if the cell comprises two attP sequences, the Int is Int-h or Int-h.218.