Patent ID: 7795002

Claim:
A recombinant yeast or filamentous fungus host cell producing recombinant glycoproteins, the host cell genetically engineered to produce N-linked oligosaccharides having terminal GlcNAc residues and comprising a nucleic acid encoding a fusion protein that in the host cell transfers a galactose residue from UDP-galactose onto a terminal GlcNAc residue of an N-linked oligosaccharide branch of an N-glycan of a glycoprotein, wherein the N-linked oligosaccharide branch is selected from the group consisting of GlcNAcβ1,2-Manα1; GlcNAcβ1,4-Manα1,3, GlcNAcβ1,2-Manα1,6, GlcNAcβ1,4-Manα1,6 and GlcNAcβ1,6-Manα1,6, wherein the host cell is diminished or depleted in dolichyl-P-Man:Man5GlcNAc2-PP-dolichyl alpha-1,3 mannosyltransferase activity, and wherein the host cell produces a glycoprotein having one or more galactose residues.