Patent ID: 6977294

Claim:
A process for the isolation of 3-O-β-D-galactopyranoside of the formula 1 from aerial parts of Boerhaavia diffusa , comprising: (a) powdering the aerial parts of, (b) preparing an extract of the powdered aerial parts of with a protic solvent, (c) concentrating the extract obtained in step (b) above, (d) triturating the above said concentrated extract successively with a series of at least three organic solvents in order of increasing polarity, the solvents being selected from the group consisting of hexane, dichloromethane, chloroform, ethyl acetate, ethanol, methanol and aqueous methanol in order of increasing polarity to get an enriched bioactive fraction, (e) crystallizing and recrystallizing the bioactive fraction with methanol to obtain 3-O-β-D-galactopyranoside,