Patent ID: 6921669

Claim:
A surface carrying a linker system comprising a compound for activating surfaces for bioconjugation having the following general formula (I): X-[(Y 1 ) i —Q—(Y 2 ) j ] k —Z (I) wherein: X is a reactive group capable of covalently binding to a surface and is selected from the group consisting of a SiW 3 group with W being a hydrolyzable atom or group, a group capable of forming free radicals on exposure to light, an anthrathione group or a derivative thereof, an anthraquinone group or a derivative thereof, and a benzophenone group or a derivative thereof; Z is a reactive group capable of covalently binding to a biomolecule, is capable of nucleophilic substitution reactions, nucleophilic addition reactions, Diels-Alder reactions or radical substitutions, and is selected from the group consisting of a diene group, a dienophilic group, an aldehyde group, a hydroxyl group, a carboxylic acid group, an active ester group, an amino group, a thiol group, an aziridine group, an isocyanate group, an isothiocyanate group, an azide group, and a reactive leaving group; X is not Z; Y 1 and Y 2 are, independently from each other, CR 1 R 2 ; R 1 and R 2 are, independently from each other, H, C 1 -C 4 alkyl, C 1 -C 4 alkoxy or C 1 -C 4 acyloxy; i, j, and k are, independently from each other, an integer in the range from 1 to 10; the total number of C atoms in Y 1 and Y 2 , the C atoms of R 1 and R 2 not included, is in the range of 2 to 100; Q is a hydrophilic atom or group selected from the group consisting of O, NH, C═O, O—C═O and CR 3 R 4 ; R 3 and R 4 are, independently from each other, selected from the group consisting of H, OH, C 1 -C 4 alkoxy and C 1 -C 4 acyloxy; and R 3 and R 4 are not H at the same time; wherein when Q=NH, Z is not NH 2 ; wherein when k>1, the Q's for each [(Y 1 ) i —Q—(Y 2 ) j ] k are independently selected from each other; and wherein the linker system is covalently bonded to a biomolecule wherein said biomolecule is a partner of a specifically interacting system of complementary binding partners; wherein said specifically interacting system of complementary binding partners is based on nucleic acid/complementary nucleic acid, peptide nucleic acid/nucleic acid, enzyme/substrate, receptor/effector, lectin/sugar, antibody/antigen, avidin/biotin or strept-avidin/biotin interaction; and wherein said antibody is a polyclonal, monoclonal, chimeric or single-chain antibody or a functional fragment or derivative of such antibodies.