Patent ID: 8771974

Claim:
A method of determining the presence of and/or measuring the amount of a biomarker to determine the presence, identity, and/or severity of the disease or condition in an individual having a disease or condition associated with abnormal glycan biosynthesis, degradation, or accumulation, the method comprising: (a) generating the biomarker comprising of one or more non-reducing end glycan residual compound(s), wherein the biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme(s), wherein prior to enzyme treatment, the biomarker is not present in abundance in samples from individuals with the disease or condition relative to individuals without the disease or condition, and (b) using an analytical instrument to detect the presence of and/or measure the amount of the biomarker produced and displaying or recording the presence of or a measure of a population of the biomarker; and (c) determining the presence, identity, and/or severity of the disease or condition utilizing the presence of and/or measure the amount of the biomarker; wherein the disease or condition is a lysosomal storage disorder or a neurological disorder; and wherein when the lysosomal storage disorder is an MPS disorder, then the digesting glycan enzyme is not a lyase.