Patent ID: 7410775

Claim:
A method for making a recombinant peptide or protein comprising: (a) producing a fusion protein comprising, at the NH 2 -terminal end of a peptide or protein of interest, an amino acid sequence containing a dipeptide site hydrolysable by a soluble Kluyveromyces lactis Kex1 endoprotease free from its transmembrane domain; (b) incubating the fusion protein of step (a) in the presence of a soluble Kluyveromyces lactis Kex1 endoprotease consisting of the amino acid sequence set forth as amino acid residues 103 through 600 of SEQ ID NO 3, or amino acid residues 103 through 611 of SEQ ID NO 3; and (c) separating and purifying the peptide or protein of interest.