Patent ID: 7811754

Claim:
A method of detecting a single nucleotide polymorphism (SNP) in a gene of interest comprising: immobilizing on a planar waveguide a plurality of wildtype probes and a plurality of SNP probes, wherein the wildtype and SNP probes are 15-25 nucleotides, the wildtype probes comprise sequences complementary to a wildtype sequence of the gene of interest and the SNP probes comprise sequences complementary to a mutant sequence having a SNP in the gene of interest; flowing an analyte with a fluorescent label over the planar waveguide, wherein the analyte comprises at least a portion of the gene of interest; detecting binding of the analyte to one or more wildtype probes of said plurality of wildtype probes and binding of the analyte to one or more SNP probes of said plurality of SNP probes by detecting fluorescent changes of the fluorescent label of the analyte, wherein the binding of the analyte to said one or more wildtype probes and the binding of the analyte to said one or more SNP probes cause the fluorescent changes of the fluorescent label of the analyte; comparing hybridization rates of the analyte binding to said one or more SNP probes with hybridization rates of the analyte binding to said one or more wildtype probes under hybridization conditions, wherein said SNP is detected in said gene of interest when the hybridization rate of the binding of the analyte to one wildtype probe from said one or more wildtype probes is significantly lower than the hybridization rate of the binding of the analyte to one SNP probe from said one or more SNP probes and the one wildtype probe from said one or more wildtype probes and the one SNP probe from one or more SNP probes differ by only one base.