Patent ID: 7094400

Claim:
A method of transferring a plasmid containing a DNA sequence coding for a protein into a recipient subject comprising: (a) transfecting autologous somatic cells in vitro with a plasmid containing a DNA sequence coding for a protein and which comprises a promoter by chemical or physical techniques to introduce the plasmid containing a DNA sequence coding for a protein into the cells; (b) screening the resulting transfected somatic cells in vitro to select a cell, wherein the selected cell is stably transfected with the plasmid containing a DNA sequence coding for a protein so that the selected cell has the permanent capacity to direct expression of the DNA sequence coding for a protein; and (c) cloning and expanding the selected somatic cell in vitro; and (d) injecting the resulting transfected, isolated, autologous, screened, cloned, and expanded somatic cells into the recipient subject.