Patent ID: 8236530

Claim:
A method of producing a monoclonal antibody, comprising the steps of: fusing a B cell of a transgenic mouse which expresses human antibodies with a myeloma cell line incapable of producing antibodies to obtain a hybridoma, wherein the hybridoma comprises a rearranged immunoglobulin heavy chain nucleotide sequence which expresses a heavy chain polypeptide, and a rearranged immunoglobulin light chain nucleotide sequence which expresses a light chain polypeptide; introducing into the hybridoma an exogenous nucleotide sequence which encodes a heavy chain polypeptide identical to the heavy chain polypeptide expressed by the rearranged immunoglobulin heavy chain nucleotide sequence to thereby obtain a transformant hybridoma transformed with the exogenous nucleotide sequence wherein the exogenous nucleotide sequence does not encode the light chain polypeptide; culturing the transformant in a cell culture medium; and obtaining a monoclonal antibody produced by the transformant.