Patent ID: 8338101

Claim:
An in vitro method for designing a drug regimen for an HIV-infected patient by determining the phenotypic susceptibility of HIV to at least one drug, comprising: i) using at least one sample comprising HIV RNA from a patient, wherein the sample comprises the complete HIV gag-pol coding region; ii) reverse-transcribing the HIV RNA and amplifying the resulting reverse transcript with primers specific for the complete HIV gag-pol coding region to obtain at least one amplicon comprising the complete HIV gag-pol coding region; iii) obtaining a plasmid having the sequence of SEQ ID NO: 49; iv) preparing at least one recombinant virus by recombination or ligation between at least one amplicon obtained in step ii) and the plasmid obtained in step iii); and v) monitoring at least one recombinant virus in the presence of at least one drug to determine the phenotypic susceptibility of said recombinant virus to at least one drug, wherein said susceptibility is determined by the cytopathogenicity of said recombinant virus to cells or by determining the replicative capacity of said recombinant virus in the presence of at least one drug.