Patent ID: 8470314

Claim:
A method for treating a blood cancer, a solid tumor, or a metastasis in a subject comprising administering to said subject a therapeutically effective amount of a composition comprising a fusion protein comprising: a) a modified diphtheria toxin or fragment thereof comprising an amino acid sequence that is at least 90% identical to the full length sequence of SEQ ID NO: 2 or 200 and comprising at least one modified T-cell epitope core comprising at least one amino acid modification in a sequence selected from: i) amino acid residues 97-105 of SEQ ID NO: 2 or 200; ii) amino acid residues 107-115 of SEQ ID NO: 2 or 200; iii) amino acid residues 116-124 of SEQ ID NO: 2 or 200; iv) amino acid residues 124-132 of SEQ ID NO: 2 or 200; v) amino acid residues 148-156 of SEQ ID NO: 2 or 200; and vi) amino acid residues 298-306 of SEQ ID NO: 2 or 200; wherein said modified diphtheria toxin or fragment thereof is cytotoxic and exhibits reduced immunogenicity compared to an unmodified diphtheria toxin; and b) at least a cell binding domain of a ligand that binds to a target cell of said blood cancer, solid tumor, or metastasis.