Patent ID: 8614081

Claim:
An engineered nitrilase polypeptide capable of converting the substrate of structural formula (I) to the product of structural formula (II): wherein R 1 is a substituted or unsubstituted phenyl; a substituted or unsubstituted phenylalkyl(C 1 -C 3 ); a substituted or unsubstituted alkyl(C 3 -C 8 ) or a substituted or unsubstituted heteroalkyl, which polypeptide has improved stability to conditions of 3 hrs at 40° C. and/or 3 hrs in 10% methanol as compared to the polypeptide of SEQ ID NO:2, comprises an amino acid sequence that is at least 80% identical to the sequence of SEQ ID NO: 122 and comprises the following residue differences as compared to SEQ ID NO:2: X139 is F and X166 is L.