Patent ID: 7501250

Claim:
A blotting method without the process of blocking for analyzing nucleic acid comprising the following steps: (1) providing a substrate having a plurality of pores; (2) transferring a nucleic acid to be analyzed to the substrate and allowing absorption of the nucleic acid by the substrate; (3) fixing the nucleic acid to be analyzed on the substrate, and the substrate is a dry substrate; (4) adding a solution containing a nucleic acid probe on the dry substrate of step (3) to base-pair the nucleic acid probe with the nucleic acid to be analyzed thereon for two or more minutes, without blocking the areas of the substrate where the nucleic acid to be analyzed has not been fixed; (5) removing the nucleic acid probe which has not been annealed to the nucleic acid to be analyzed of step (4); and (6) detecting the hybridization signal on the substrate having been subjected to step (5), wherein in step (3), the substrate is dried at a temperature of 80° C. to 130° C. to fix the nucleic acid to be analyzed on the substrate and the drying time is 1 to 10 minutes.