Patent ID: 8288128

Claim:
A method for real-time quantification of multiple target molecules on a micro-array comprising the steps of: placing, in a reaction chamber, a support having fixed upon its surface a micro-array comprising at least 5 capture molecules each being immobilized in specifically localized areas of said support, introducing a solution containing labeled target molecules into the reaction chamber such that the thickness of the solution in contact with the micro-array is constant above all the localized areas, incubating said labeled target molecules under conditions allowing a specific binding between said targets and their corresponding capture molecules, directing and focusing an excitation light from a light source onto the surface of the support such that the light reaches the micro-array surface at an angle comprised between 45Â° and 135Â°, wherein the excitation is directed and focused on the support through the solution and wherein the difference of thickness of the solution above the localized areas is less than 100 Î¼m, less than 10 Î¼m or less than 1 Î¼m, measuring the light emission from bound target molecules in response to said excitation light in the presence of the solution containing the labeled target molecules, wherein the surface of emission for a localized area is comprised between about 0.1 Î¼m 2 and about 10 mm 2 and wherein each of at least 4 localized areas is monitored with time with at least two measurements being done per localized area, and processing and storing the values of the different measurements and quantifying at least 4 different target molecules present in the solution using at least one measurement value for each said target.