Patent ID: 8530217

Claim:
A method of removing an initiator methionine from a peptide, wherein position P1′ of the peptide is selected from the group consisting of Ala, Asn, Cys, Gln, Gly, His, Ile, Leu, Phe, Pro, Ser, Thr, Trp and Val, comprising contacting the peptide with an E. coli aminopeptidase variant of SEQ ID NO: 1, wherein: a) the amino acid at position 168 is selected from the group consisting of Gly, Ala and Asn; the amino acid at position 206 is Met; and the amino acid at position 233 is Gln; or b) the amino acid at position 168 is Tyr; the amino acid at position 206 is selected from the group consisting of Ala, Gly and Asn; and the amino acid at position 233 is Gln; or c) the amino acid at position 168 is Tyr; the amino acid at position 206 is Met; and the amino acid at position 233 is selected from the group consisting of Ala, Gly and Asn; or d) the amino acid at position 168 is Tyr; the amino acid at position 206 is selected from the group consisting of Ala, Gly and Asn; and the amino acid at position 233 is selected from the group consisting of Ala, Gly and Asn; under conditions suitable for the aminopeptidase variant to remove the initiator methionine, and wherein each of the positions are numbered according to SEQ ID NO: 1.