Patent ID: 7534588

Claim:
A method for identifying HBV (Hepatitis type B Virus), HCV (Hepatitis type C Virus), and HIV (Human Immunodeficiency Virus) simultaneously comprising: (a) amplifying DNA fragments of the said viruses synchronously in a sample using different outer and inner primer sets for each virus; the outer primer sets consisting of SEQ ID NO:1 and SEQ ID NO:2 for HBV, SEQ ID NO:3 and SEQ ID NO:4 for HCV, SEQ ID NO:5 and SEQ ID NO:6 for HIV; the inner primer sets consisting of SEQ ID NO:7 and SEQ ID NO:8 for HBV, SEQ ID NO:9 and SEQ ID NO:10 for HCV, and SEQ ID NO:11 and SEQ ID NO:12 for HIV; (b) hybridizing the amplified fragments with different specific probes for identifying amplified DNA fragments from HBV, HCV, and HIV, the probes consisting of SEQ ID NO:13 for HBV, SEQ ID NO:14 for HCV, and SEQ ID NO:15 for HIV; and (c) detecting the hybrid products of (b).