Patent ID: 8349910

Claim:
A method for producing a 2-formylglycine in a polypeptide, the method comprising: contacting a non-naturally occurring, recombinant polypeptide comprising a heterologous sulfatase motif with a formylglycine generating enzyme (FGE), wherein the heterologous sulfatase motif is less than 13 amino acid residues and contains a contiguous sequence of the formula X 1 Z 1 X 2 Z 2 X 3 R (SEQ ID NO: 2) (I) wherein Z 1 is cysteine or serine; Z 2 is a proline or alanine residue; X 1 , X 2 and X 3 are each independently any amino acid; and wherein said contacting is under conditions sufficient for conversion of Z 1 to a 2-formylglycine (FGly) residue in the non-naturally occurring, recombinant polypeptide, thereby producing a converted aldehyde tagged polypeptide.