Patent ID: 8603783

Claim:
A method for performing a polymerase chain reaction (PCR) in a reaction chamber, comprising: (a) placing said reaction chamber in the vicinity of a temperature-control bladder having a flexible thermal conductive surface, said thermal conductive surface forms a contact with an exterior surface of said reaction chamber when said temperature-control bladder is filled with a temperature control fluid, wherein said flexible thermal conductive surface facilitates heat transfer from said temperature control fluid to said reaction chamber; (b) filling said temperature-control bladder with said temperature-control fluid at a first temperature; (c) holding said temperature-control fluid at the first temperature in said temperature-control bladder for a first period of time; (d) filling said temperature-control bladder with said temperature-control fluid at a second temperature; and (e) holding said temperature-control fluid at the second temperature in said temperature-control bladder for a second period of time.