Patent ID: 7641922

Claim:
A method for the preparation of total salvianolic acid comprising the steps of: (a) extracting Danshen with water to produce a Danshen extract, acidifying the extract and filtering the extract; (b) applying the Danshen extract to a polyamide column, washing the polyamide column with water until neutral, discarding the washings, and collecting fractions eluted from the polyamide column with a weak basic aqueous solution; (c) acidifying the fractions eluted from the polyamide column, applying the fractions to a macroporous adsorption resin column, washing the column with water to achieve neutrality, discarding the washings, and collecting eluate from the macroporous adsorption resin column with a hydrous or anhydrous lower alcohol, wherein the eluate is further optionally concentrated and dried.