Patent ID: 7807398

Claim:
A method for in situ production of an emulsifier in a foodstuff, comprising: adding a lipid acyltransferase to the foodstuff, whereby, when in contact with the foodstuff, the lipid acyl transferase through its acyl transferase activity transfers an acyl group from a lipid to one or more acceptor substrates having a hydroxy group to form a least one emulsifier, wherein: the lipid acyltransferase comprises the amino acid sequence motif GDSX, X is an amino acid residue selected from the group consisting of: L, A, V, I, F, Y, H, Q, T, N, M and S and the lipid acyltransferase when tested using the Transferase Assay in Buffered Substrate has at least 5% acyltransferase activity (relative acyltransferase activity), wherein the Transferase Assay in Buffered Substrate comprises: (a) heating to 35° C. a substrate solution comprising phosphatidylcholine, cholesterol, water and HEPES buffer, wherein the substrate solution comprises approximately 95% water and has pH 7.0; (b) adding an enzyme to the substrate solution; and (c) determining acyltransferase activity of the enzyme based upon cholesterol ester and fatty acids formed.