Patent ID: 7897379

Claim:
A manifold for directing in-flow of liquid and out-flow of gas through a cell culture vessel comprising: a necked opening comprising an opening and an interior surface; an inflow shoulder and an outflow shoulder; a barrier plate structured and arranged to attach to a cell culture vessel; wherein the inflow shoulder and outflow shoulder are attached to the barrier plate and to the neck; wherein the inflow shoulder and outflow shoulder each have interior surfaces; wherein the interior surface of the neck is adjacent to and continuous with the interior surfaces of the inflow shoulder and the outflow shoulder; wherein the inflow shoulder and the outflow shoulder comprise at least one aperture through the barrier plate; whereby liquid introduced into the necked opening flows through the necked opening into the inflow shoulder, through the inflow aperture into the cell culture vessel, and gas displaced by the liquid entering the cell culture vessel is vented out of the cell culture vessel through the aperture of the outflow shoulder, into the outflow shoulder, and out through the necked opening.