Patent ID: 8202687

Claim:
A method for transporting potassium ions from a front side of a lipid bilayer membrane to a back side thereof, the method comprising the following steps (a) and (b): step (a) of preparing the lipid bilayer membrane, wherein the lipid bilayer membrane comprises a G protein-coupled receptor, a G protein, and a G protein-coupled inwardly rectifying potassium ion channel, the G protein comprises a chimeric G α subunit and a G βγ subunit complex, and the chimeric G α subunit consists of any one of G i/olf13 (SEQ ID NO: 04), G i/olf28 (SEQ ID NO: 05), G i/olf94 (SEQ ID NO: 07), G i/olf113 (SEQ ID NO: 08), G i/olfα3-β5,C (SEQ ID NO: 12), or G i/olfα4-β6,C (SEQ ID NO: 15); and step (b) of supplying an agonist of said G protein-coupled receptor and the potassium ions to the front side to release the chimeric G α subunit and G βγ subunit complex, and to allow the G βγ subunit complex to bind to the potassium ion channel for transporting the potassium ions from the front side to the back side.