Patent ID: 6897058

Claim:
A method for the preparation of an enantiomerically enriched acylated amide of formula 1, wherein R 4 is a substituted or unsubstituted phenyl group, X is H, OH, NH 2 , NHOH, F, Cl, Br, NO 2 , alkyl or alkoxy with 1-3 C-atoms, and each of R 1 , R 2 , R 3 is different from the other two, and each is independently H, CN, alkoxy, alkyl, cycloalkyl, aryl, alkylaryl, arylalkyl, or a cyclic or noncyclic heteroalkyl or heteroaryl group with one or more N, O or S atoms, each of which may be optionally substituted with halo, hydroxy, alkoxy, aryloxy, thio, alkylthio, arylthio, cyano, nitro, amino, dialkylamino, or amido; wherein a phenylacetic acid derivative of formula 2, wherein R 4 and X are as defined above, and Z is a C1-C7 alkoxy group; NR 6 R 7 , where R 6 and R 7 are each independently H, a substituted or unsubstituted alkyl, cycloalkyl, or aryl group; NHNH 2 ; or NHOR 8 , where R 8 is H, or a C1-C7 alkyl group, is contacted with a mixture of enantiomers of the amine H 2 NCR 1 R 2 R 3 , where R 1 , R 2 and R 3 , are as defined above, in the presence of a Pen-G acylase originating from Alcaligenes faecalis.