Patent ID: 6872808

Claim:
An isolated protein having anticoagulant activity and having one or more Nematode Anticoagulant Protein (NAP) domains, wherein each NAP domain includes the sequence: Cys-A1-Cys-A2-Cys-A3-Cys-A4-Cys-A5-Cys-A6-Cys-A7-Cys-A8-Cys-A9-Cys-A10 (FORMULA III), wherein (a) A1 is an amino acid sequence of 7 to 8 amino acid residues; (b) A2 is an amino acid sequence; (c) A3 is an amino acid sequence of 3 amino acid residues and has the sequence Asp-A3 a ,-A3 b wherein A3 a and A3 b are independently selected; (d) A4 is an amino acid sequence having a net anionic charge; (e) A5 is an amino acid sequence of 4 amino acid residues and has the sequence A5 a ,-A5 b -, A5 c -A5 6 (SEQ ID NO: 85), wherein A5 a through A5 d are independently selected amino acid residues; (f) A6 is an amino acid sequence; (g) A7 is an amino acid residue selected from the group consisting of Val and Ile; (h) A8 is an amino acid sequence of 11 to 12 amino acid residues; (i) A9 is amino acid sequence of 5 to 7 amino acid residues; and (j) A10 is an amino acid sequence; when each of A2, A4, A6 and A10 has an independently selected number of independently selected amino acid residues and each sequence is selected such that each NAP domain has in total less than 120 amino acid residues, and wherein said protein has a NAP domain with an amino acid sequence having at least 90% sequence identity with the NAP domain of AcaNAPc2, wherein AcaNAPc2 has the amino acid sequence sat forth in SEQ ID NO: 59.