Patent ID: 7238378

Claim:
A variant of a parent phytase, comprising one or more of the following sets of substitutions: 29N+102I; 29N+118A; 29N+163P+324S; 29N+163P+324S+395G; 29N+234S; 29N+324S; 29S+45A+69A+99D+110Y+125D+324A+334E+344S+350V; 29S+63N+125D+183K+218T+324A+334E+350V+421S; 29S+95H+110W+183W+324A+350V; 29S+95M+110W+183W+324A+350V; 29S+95N+110R+183W+324A+350V; 29S+95R+110W+183W+324A+350V; 29S+95S+110R+183W+324A+350V; 29S+95S+110W+183W+324A+350V; 29S+95T+110R+183W+324A+350V; 29S+95T+110Y+125D+183W+286S+324A+350V; 29S+95T+110Y+152A+156C+157Q+160R+183W+324A+350V; 29S+95T+110Y+156R+157Q+183W+324A+350V; 29S+95T+110Y+157K+183W+185H+187R+190S+191R+248G+324A+350V; 29S+95T+110Y+183W+185A+248A+324A+350V; 29S+95T+110Y+183W+185A+248C+324A+350V; 29S+95T+110Y+183W+185A+248E+324A+350V; 29S+95T+110Y+183W+185A+248H+324A+350V; 29S+95T+110Y+183W+185E+248S+324A+350V; 29S+95T+110Y+183W+185H+248G+321A+323A+324A+350V; 29S+95T+110Y+183W+185H+248G+324A+350V; 29S+95T+110Y+183W+185H+187S+190K+191S+210G+222I+248G+324A+350V; 29S+95T+110Y+183W+185K+248G+324A+349K+350L; 29S+95T+110Y+183W+185K+248S+324A+350V; 29S+95T+110Y+183W+185K+248T+324A+349A+350L; 29S+95T+110Y+183W+185K+248T+324A+349L+350I; 29S+95T+110Y+183W+185K+248T+324A+349L+350M; 29S+95T+110Y+183W+185K+248T+324A+350V; 29S+95T+110Y+183W+185P+248G+324A+350V; 29S+95T+110Y+183W+185P+248S+324A+350V; 29S+95T+110Y+183W+191L+324A+350V; 29S+95T+110Y+183W+248D+324A+350V; 29S+95T+110Y+183W+324A; 29S+95T+110Y+183W+324A+350V; 29S+99D+125D+324A+334E+350V; 29S+110Y+183W+185N+324A+350V; 29S+125D+218T+324A+344S+350V; 29S+125D+234K+324A+330R; 29S+125D+234K+324A+330R+395G; 29S+125D+324A; 29S+125D+324A+330R+395G; 29S+125D+324A+350V; 29S+183G+324A+334E+344S+350V; 29S+205S; or 29T+324L; wherein (a) the variant has phytase activity; (b) each position corresponds to a position of SEQ ID NO: 2; (c) the variant has an amino acid sequence which has a degree of identity of at least 90% to SEQ ID NO: 2.