Patent ID: 7833530

Claim:
A method of inhibiting PSGL-1-mediated adhesion and chemokine-mediated migration of human leukocytes, lymphocytes or endothelial cells, comprising: providing a PSGL-1-specific monoclonal antibody or a binding fragment thereof which binds with high affinity to a sulfated N-terminal portion of human PSGL-1 comprising at least a portion of amino acids 42-62 of SEQ ID NO:1; and exposing the antibody or binding fragment thereof to PSGL-1-bearing human leukocytes, lymphocytes or endothelial cells wherein the antibody or binding fragment thereof binds to the sulfated N-terminal portion of the human PSGL-1 on the human leukocytes, lymphocytes or endothelial cells and blocks the binding of P-selectin and/or L-selectin to the human PSGL-1 on the human leukocytes, lymphocytes, or endothelial cells and blocks the binding of at least one of chemokines CCL19, CCL21, CCL27 and CCL28 to the human PSGL-1 on the human leukocytes, lymphocytes or endothelial cells, thereby inhibiting both adhesion and chemotactic migration of the human leukocytes, lymphocytes or endothelial cells.