Patent ID: 8748698

Claim:
A genome shuffling method for autogamous plants, comprising: producing dominant male-sterile individuals by transforming a plant with a vector having the following three gene cassettes as a single constructs: 1) a male-sterility gene cassette including an anther-specific promoter and a ribonuclease-encoding gene that is driven by the anther-specific promoter, 2) a chemical tolerance gene cassette including a constitutive promoter and a chemical tolerance marker gene driven by the constitutive promoter, wherein the constitutive promoter allows expression of the chemical tolerance marker gene in stems and leaves, and 3) a susceptibility gene cassette to physical environment or a specific chemical including a gene for destroying intrinsic tolerances to the environment or chemicals, arranging cultivars or strains intended for genome shuffling near the dominant-male sterile individuals in a flowering period of each of (1) the cultivars or strains intended for genome shuffling and (2) the dominant-male sterile individuals, so that the flowering periods of (1) and (2) coincide with one another, harvesting outcrossed seeds from the dominant-male sterile individuals, segregating the outcrossed seeds into a first group and a second group, subjecting the first group to screening in terms of chemical tolerance, thereby allowing selection of male-sterile individuals at the seedling stage, subjecting the second group to destruction of intrinsic tolerances to the environment or chemicals, thereby efficiently selecting male-fertile individuals at the seedling stage, arranging the male-sterile individuals and the male-fertile individuals close together in flowering periods thereof, so that the male-sterile individuals are capable of crossing with the male-fertile individuals, harvesting seeds from the male-sterile individuals, and repeating outcrossing using the seeds from generation to generation.