Patent ID: 8071307

Claim:
A method of discriminating a relative risk for morbidity of atopic dermatitis of a test subject, comprising (i) a step of preparing a sample which contains a gene as an object of analysis and derives from a test subject and (ii) a step of determining individual gene polymorphism in combination with any two steps selected from steps (a) to (e) below: (a) a step of analyzing gene polymorphism of human mast cell chymase gene extracted from the sample isolated from the test subject by determining which of guanine and adenine is the 3255th nucleotide of the nucleotide sequence represented by SEQ ID NO: 1; (b) a step of analyzing gene polymorphism of human high affinity IgE receptor Î²-chain (FcÎµRIÎ²) gene extracted from the sample isolated from the test subject by determining which of guanine and adenine is the 1343rd nucleotide from the initiation codon of the nucleotide sequence represented by SEQ ID NO: 2; (c) a step of analyzing gene polymorphism of RANTES gene extracted from the sample isolated from the test subject by determining which of guanine and adenine is the âˆ’403rd nucleotide at the upstream side counted from the transcription initiation site of mRNA of the nucleotide sequence represented by SEQ ID NO: 3; (d) a step of analyzing gene polymorphism of RANTES gene extracted from the sample isolated from the test subject by determining which of guanine and cytosine is the âˆ’28th nucleotide at the upstream side counted from the transcription initiation site of mRNA of the nucleotide sequence represented by SEQ ID NO: 3; and (e) a step of analyzing gene polymorphism of interleukin-13 gene extracted from the sample isolated from the test subject by determining which of guanine and adenine is the 4257th nucleotide of the nucleotide sequence represented by SEQ ID NO: 4.