Patent ID: 7270983

Claim:
A method for preparing a multiplex, parallel assay to identify the source of body fluids from a human being selected from the group consisting of one or more of blood, saliva, semen and vaginal secretions, comprising the steps of: a) obtaining a sample stain consisting of a single body fluid or multiple fluids from a human being; b) extracting total ribonucleic acid (RNA) from the sample stain; c) treating the total RNA with an enzyme; d) initiating a reverse-transcriptase (RT) reaction by treating total RNA with random decamers to produce cDNA; e) amplifying said cDNA using gene-specific primer pairs for each of the genes SPTB, PBGD, STATH, HTN3, PRM1, PRM2, MUC4, and HBD-1, wherein each gene is related to a specific human body fluid; SPTB and PBGD being genes specific for blood; STATH and HTN3 being genes specific for saliva; PRM1 and PRM2 being genes specific for semen; and HBD-1 and MUC4 being genes specific for vaginal secretions; f) controlling the size of the amplified cDNA to allow separation by electrophoresis; and g) identifying the body fluid-specific genes present in the sample, wherein the presence of one or more of said body fluid specific genes identifies the source of the body fluid(s) in the sample.