Patent ID: 7226754

Claim:
A method for identifying a compound which modulates kinase Wee1 activity comprising the steps of: a) combining: i) a test compound; ii) a glutathione-Stransferase (GST) :Wee1 fusion protein comprising the amino acid residue sequence in SEQ ID NO: 1; and iii) a reagent for Wee1 b) maintaining the combination of step (a) under conditions suitable for Wee1 to react with said reagent; and c) determining the amount of phosphorylation of said Wee1fusion protein in the presence of the test compound and in the absence of the test compound, whereby a difference in the amount of reaction phosphorylation in the presence of the test compound and in the absence of the test compound indicates that said test compound modulates the activity of Wee1.