Patent ID: 8609824

Claim:
A method for preparing heat-treated, fractionated thymus extract composition, comprising: homogenizing thymus tissue with aqueous hypotonic homogenization fluid to produce an aqueous thymus homogenate; removing tissue debris from the aqueous thymus homogenate to produce a primary supernatant; heat denaturing the primary supernatant, and clarifying the denatured primary supernatant by use of at least one of low-speed centrifugation and filtration, to produce a clarified supernatant; separating molecules having molecular weights less than 3.5 kDa from the clarified supernatant; and separating molecules having molecular weight greater than about 30 kDa from the heat-treated, fractionated thymus extract composition, wherein a heated, fractionated thymus extract composition comprising proteins or polypeptides having molecular weights in the range of 3.5 kDa to 30 kDa is provided.