Patent ID: 8921103

Claim:
A method of preparing a dermal equivalent comprising: providing a synthetic hydrogel matrix comprising at least a first synthetic hydrogel layer and a second synthetic hydrogel layer, wherein the first synthetic hydrogel layer comprises at least one material selected from the group consisting of fibrin, PEGylated fibrin, hyaluronic acid, and any combination thereof and wherein the second synthetic hydrogel layer comprises at least one material selected from the group consisting of collagen I, collagen II, collagen III, collagen IV, collagen V, fibronectin, tenascin, vitronectin, glycosaminoglycans, and any combination thereof; isolating a population of mesenchymal stem cells from a source; and differentiating at least one of the mesenchymal stem cells towards a dermal fibroblast and at least one of the mesenchymal stem cells towards a blood vessel cell by introducing the population of mesenchymal stem cells between the first and second synthetic hydrogel layers.