Patent ID: 8546346

Claim:
A method of repressing the use of a splice site of a target pre-mRNA molecule, said method comprising forming a complex between an oligonucleotide, said target pre-mRNA molecule and a hnRNP protein in a cell or a cell extract, wherein said oligonucleotide comprises at least two different sequence elements: (i) a single stranded RNA having between 18 and 20 nucleotides and being complementary to a region upstream of said splice site in said target pre-mRNA molecule, said region being located between said splice site and 46 nucleotides upstream of said splice site; wherein the 5′ end of said single stranded RNA is operatively linked to (ii) a single stranded nucleic acid extension having between 16 and 20 nucleotides and containing a hnRNP protein binding site sequence element for binding said hnRNP protein.