Patent ID: 8017132

Claim:
An isolated or purified polypeptide which is a mutant of an adenylate cyclase protein and whose amino acid sequence comprises or consists of one of the following sequences: a) the amino acid sequence of the adenylate cyclase (CyaA) of SEQ ID NO: 1, SEQ ID NO: 10, or SEQ ID NO: 11, wherein the following mutations have been performed: (i) the substitution of the glutamic acid residue at position 570 by a glutamine residue (E570Q), and (ii) the substitution of the lysine residue at position 860 by an arginine residue (K860R), or b) the amino acid sequence of the adenylate cyclase (CyaA) of SEQ ID NO: 12, wherein the following mutations have been performed: (i) the substitution of the glutamic acid residue at position 569 by a glutamine residue (E569Q), and (ii) the substitution of the lysine residue at position 859 by an arginine residue (K859R).