Patent ID: 8748192

Claim:
An optical fluorescence dual sensor comprising: (a) a probe for sensing pH; (b) a probe for sensing oxygen; (c) an intra-reference probe; and (d) a matrix; wherein the probe for sensing pH has formula I: wherein R 1 is C n H 2n +1 , where n is an integer selected from the group consisting of 1, 2, 3, 4, 5, 6, 7 and 8; or C m H 2m X, where m is an integer selected from the group consisting of 2, 3, 4, 5, 6, 8 and 11; R 2 is C m H 2m X, where m is an integer selected from the group consisting of 2, 3, 4, 5, 6, 8 and 11; and each X is independently selected from the group consisting of the probe for sensing oxygen has formula II: where M is selected from Pt or Pd; R 11 and R 12 can be the same or different and are independently selected from the group consisting of H, F, Cl, Br, I, CH 3 , OCH 3 and OC 2 H 5 ; R 3 and R 4 can be the same or different and are independently selected from the group consisting of H, F, Cl, Br, I, CH 3 , OCH 3 and OC 2 H 5 ; R 5 and R 6 can be the same or different and are independently selected from the group consisting of H, F, Cl, Br, I, CH 3 , OCH 3 and OC 2 H 5 ; R 7 , R 8 , R 9 and R 10 can be the same or different and are independently selected from the group consisting of (CH 2 ) p OH, O(CH 2 ) p OH, NH(CH 2 ) p OH, (CH 2 ) p OM′A, O(CH 2 ) p OM′A, NH(CH 2 ) p OM′A, (CH 2 ) p OA, O(CH 2 ) p OA, NH(CH 2 ) p OA, (CH 2 ) p OVA, O(CH 2 ) p OVA, NH(CH 2 ) p OVA, (OCH 2 CH 2 ) q OH, NH(CH 2 CH 2 O) q H, (OCH 2 CH 2 ) q OM′A, NH(CH 2 CH 2 O) q M′A, (OCH 2 CH 2 ) q OA, NH(CH 2 CH 2 O) q A, (OCH 2 CH 2 ) q OVA, NH(CH 2 CH 2 O) q VA, where M′A is A is VA is and p is an integer selected from the group of consisting of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, q is an integer selected from the group of consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 38, 39, 40, 41, 42, 43, 44, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149 and 150; the intra-reference probe has formula III: wherein each X is independently selected from the group consisting of: and n is an integer selected from 1-100; and the matrix comprises poly(2-hydroxyethyl methacrylate), polyacrylamide, and poly(2-hydroxyethyl methacrylate)-co-polyacrylamide (PHEMA-co-PAM).