Patent ID: 7718437

Claim:
Method for analyzing adducts in a fluid and/or solid material suspected of containing said adducts, wherein said adduct is an N-adducted amino acid or adducted N-terminal peptide/protein, comprising the following steps: a) bringing said fluid and/or solid material in direct contact with an isothiocyanate reagent wherein said reagent is an isothiocyanate reagent containing a fluorescent moiety and an ionizable moiety selected from the group consisting of FITC, DNITC and DABITC or a derivative thereof; b) allowing said reagent to react with adducted N-terminals in proteins or peptides present in said fluid and/or solid material; c) separating the analytes formed from the reaction mixture; and d) detecting the analytes formed, and optionally visualizing the result, wherein step c) is performed using LC and step d) is performed using MS detection.