Patent ID: 7147762

Claim:
A method of electrophoretic separation of one or more protein and/or peptide components of a sample, which method comprises (a) contacting the sample with a convection stabilised separation medium; (b) applying a voltage across said medium; and (c) observing the results of the separation obtained by analysis of one or more sections of the separation medium; wherein a disulphide-containing compound selected from the group consisting of bis-(2-hydroxyethyl) disulphide; bis-(2-hydroxypropyl) disuiphide; 3,3-dipropionamidedisulphide and 2,2′-dipyridyl disulphide is added to the separation medium or to the sample before step (a) in an amount sufficient to provide an excess of reactive disulphide groups during the separation and to prevent protein agglutination and streaking.