Patent ID: 8796006

Claim:
A method of preparing a crystal of the N-domain of ACE protein consisting of an amino acid sequence defined by amino acids 1-629 of SEQ ID NO: 8, the method comprising the steps of: (a) culturing host cells that express the N-domain of ACE protein; (b) purifying the N-domain of ACE protein; and (c) crystallising the N-domain of ACE protein using about 5 mM HEPES and about 0.1 mM phenylmethylsulfonylfluoride (PMSF) with an equal volume of a reservoir solution containing about 0.2M lithium sulphate, 15% PEG 4000, about 100 mM CH 3 COONa.3H 2 O pH 4.9 and about 10 μM ZnSO 4 .7H 2 O.