Patent ID: 7615351

Claim:
A method of detecting one or more nucleic acids of interest, which method comprises: (a) providing a sample comprising or suspected of comprising one or more nucleic acids of interest; (b) capturing the one or more nucleic acids of interest on a first solid support; (c) hybridizing one or more first amplification multimers to each of the one or more nucleic acids of interest; (d) hybridizing one or more amplification oligomers to the one or more first amplification multimers of step (c); (e) transferring the one or more amplification oligomers that hybridized in step (d) to a second solid support; (f) hybridizing one or more second amplification multimers to the one or more amplification oligomers captured on the second solid support; (g) hybridizing one or more label probes to the one or more second amplification multimers of step (f); and, (h) detecting the one or more label probes.