Patent ID: 7459698

Claim:
Process for the observation of at least one sample region with a light raster microscope having an optical axis, comprising the steps of: (a) generating illumination light for illuminating a sample, (b) moving the illumination light and the sample relative to each other along at least one scanning axis substantially perpendicular to the optical axis, using a first scanner, (c) moving a second scanner at an angle to the plane of the relative movement, wherein the first and second scanners are independent of each other, and are provided at least partially in duplicate in a first and second system which are directed at least one of simultaneously and sequentially onto the same samples, (d) acquiring an image by coupling the movements of the first and second scanners and performing a three-dimensional sampling movement by illumination into an illumination region of the sample, and (e) carrying out at least one of an independent and common detection of the sample light, wherein in case of a detected change within the observed illumination region by the first or second system, the observed illumination region of the other system is changed by a control unit common to both systems.