Patent ID: 8093057

Claim:
An assay system comprising: an assay device comprising: a pad configured to receive a sample and a detector for providing a mixture comprising the sample and the detector, the sample comprising glycohemoglobin and non-glycohemoglobin, the detector having specificity in binding with glycohemoglobin while having little specificity in binding with non-glycohemoglobin, wherein the detector is included in the mixture in a predetermined amount that is more than sufficient to bind with substantially all glycohemoglobin; a strip comprising a chromatographic medium configured to receive the mixture from the pad and to let the mixture migrate in a direction in the chromatographic medium; a first detection zone provided in the strip and distanced at a first predetermined distance from the pad in the direction, wherein the first detection zone comprises a captor having specificity in binding with the detector, wherein the captor is immobilized in the first detection zone and provided in an amount that is sufficient to capture substantially all the detector that are not bound with glycohemoglobin and reach the first detection zone, wherein the first detection zone provides an access for detecting an amount of the detector captured in the first detection zone; and a second detection zone provided in the strip and distanced at a second predetermined distance from the pad in the direction, wherein the second detection zone provides an access for detecting an amount of non-glycohemoglobin and glycohemoglobin that are staying or passing in the second detection zone at a detection time; a first detection device configured to irradiate first light beams to the first detection zone and to detect fluorescence from a fluorescent moiety bound to the detector in response to the first light beams; and a second detection device configured to irradiate second light beams to the second detection zone and to detect light emitted from non-glycohemoglobin and glycohemoglobin in response to the second light beams.