Patent ID: 8247172

Claim:
A method of amplifying one or more target regions in a nucleic acid sample comprising; (i) providing forward and reverse compound primers immobilised on the surface of a solid support, wherein said forward and reverse compound primers comprise a common amplification sequence and a specific primer sequence, the specific primer sequences of the immobilised forward and reverse compound primers being hybridisable to the one or more target regions of the nucleic acid sample, (ii) hybridising a non-priming barrier oligonucleotide to the common sequences of the immobilised forward and reverse compound primers, (iii) hybridising a template strand of the nucleic acid sample to the specific primer sequence of the forward compound primers, (iv) extending the forward compound primers along the template strand to produce immobilised first extension products comprising the compound primer and the complementary sequence of the one or more target regions of the template strand of the nucleic acid sample, (v) hybridising the first extension products to the immobilised reverse compound primers, (vi) extending the reverse compound primers along the first extension products to produce immobilised second extension products comprising the sequence of the reverse compound primer, the template strand of the nucleic acid sample in the target region and the complement of the forward compound primer, and, (vii) amplifying the immobilised second extension products using common primers which hybridise to the complement of the common sequences of the forward and reverse compound primers to produce amplified nucleic acid molecules in solution which comprise the one or more target regions.