Patent ID: 8822151

Claim:
A method of detecting at least one target double stranded nucleic acid in a sample using a point-of-care assay, the method comprising the steps of: a) partially opening the target double stranded nucleic acid into a first sink strand and a second single strand; b) exposing the sample to at least one first complex located on a chromatographic test strip, wherein the first complex comprises at least one first nucleic acid sequence associated with at least one label, and wherein the first nucleic acid sequence comprises a sequence complementary to a portion of a sequence of the first single strand of the target nucleic acid; and c) exposing the sample to at least one second complex located on the test strip, wherein the second complex comprises at least one second nucleic acid sequence, and wherein the second nucleic acid sequence comprises a sequence complementary to a portion of a sequence of the second single strand of the target nucleic acid; wherein, when the target nucleic acid is present in the sample, the first nucleic acid sequence and the second nucleic acid sequence associate with the target nucleic acid such that the first complex, the target nucleic acid, and the second complex are immobilized in the test zone at a completion of the assay.