Patent ID: 7695929

Claim:
A method for detecting two or more different targets in a sample, comprising: contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets, wherein the two or more specific binding moieties are conjugated to different haptens, at least one of the haptens being a first rotenoid having a formula where R—R 5 independently are hydrogen, aldehyde, alkoxy, aliphatic, substituted aliphatic, heteroaliphatic, amino, amino acid, amido, cyano (—CN), halogen, hydroxyl, hydroxylamine, oxime, oxime ether, alkyl hydroxyl, ketone, nitro, sulfhydryl, sulfonyl, sulfoxide, carboxyl, carboxylate, ester, alkyl ester, acyl, exomethylene, ether, cyclic, heterocyclic, aryl, alkyl aryl, heteroaryl, carbohydrate, monosaccharides, disaccharides, oligosaccharides, polysaccharides, and combinations thereof, Y is oxygen, an amine, or sulfur and at least one of the R—R 5 substituents is bonded to a linker or to a carrier molecule, the other hapten being an oxazole, a pyrazole, a thiazole, a benzofurazan, a triterpene, a urea, a thiourea, a nitroaryl other than dinitrophenyl, a second rotenoid different from the first rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azoaryl, or a benzodiazepine; contacting the sample with two or more different anti-hapten antibodies that can be detected separately; and detecting the anti-hapten antibodies.