Patent ID: 7485462

Claim:
A commercially viable process for in-vitro mass culture of Chlorophytum borivilianum of true to type clone comprising the steps of: a. isolating meristematic explants from selected parts of a mother plant; b. cleaning the explants by washing thoroughly under running water to remove soil adhering to the explants, cutting approximately 1 to 2 cm pieces of root top with crown meristem, cleaning with a mild detergent of about 0.5 to 5% Tween-20 solution with intermittent shaking for a period of 15 to 30 minutes, and then washing thoroughly with demineralized water; c. subjecting the explants to a primary sterilization by treating the explants with a solution containing a fungicide comprising Bavistin at a concentration of 0.05% to 0.2% and a bactericide including streptomycin at a concentration of 0.1% to 1% for a period of 20-80 minutes, and then rinsing with sterile water; d. subjecting the primary sterilized explants to a secondary sterilization by cutting the crown part of tuberous roots about 0.5-1 cm below the apex in a Laminar flow bench, treating with a surface sterilizing agent comprising sodium hypochlorite in a range of 0.5-2% for a period of 0.05-0.25% for a period of 2 to 5 minutes, then rinsing with sterile distilled water thrice; e. preparing an explant that is a meristematic tissue for inoculation, by trimming the explant to about 0.3 to 0.6 cm cube, without damaging the crown meristem, and isolating the meristematic tissue; f. inoculating the explants on a culture initiation medium comprising basal salts of MS medium to give multiple shoots; g. transferring the cultures to a proliferation and elongation medium; and, h. transferring the elongated shoots to a rooting medium comprising basal salts of MS medium to produce said in vitro culture of Chlorophytum borivilianum of true to type clone.