Patent ID: 7053195

Claim:
A heteropolymer for introducing a single base change in a target oligonucleotide, wherein the target oligonucleotide comprises a 3′ → 5′ strand of contiguous nucleic acids having a target sequence of nucleic acids comprising a nucleotide to be modified, wherein said heteropolymer comprises: a first strand having a sequence of nucleic acids that is completely complementary to the target sequence of nucleic acids except for a single nucleotide on the first strand at a position corresponding to the position of the nucleotide to be modified on the target sequence, wherein the first strand comprises one or more locked nucleic acids; and a second strand having a sequence of deoxyribonucleic acids that is completely complementary to the first strand, wherein at least two homologous regions of the first strand comprise at least 3 contiguous LNA-type nucleobases which are Watson-Crick paired to deoxyribo-type nucleobases of the second strand.