Patent ID: 8492338

Claim:
An underglycosylated targeted therapeutic fusion protein comprising: a lysosomal enzyme, wherein the lysosomal enzyme is selected from the group consisting of: acid-α1,4-glucosidase; β-galactosidase; β-hexosaminidase A; β-hexosaminidase B; α-galactosidase A; glucocerebrosidase; arylsulfatase A; galactosylceramidase; acid sphingomyelinase; acid ceramidase; acid lipase; α-L-iduronidase; iduronate sulfatase; heparan N-sulfatase; α-N-acetylglucosaminidase; acetyl-CoA-glucosaminide acetyltransferase; N-acetylglucosamine-6-sulfatase; galactosamine-6-sulfatase; β-galactosidase; arylsulfatase B; β-glucuronidase; α-mannosidase; β-mannosidase; α-L-fucosidase; N-aspartyl-β-glucosaminidase; α-neuraminidase; lysosomal protective protein; α-N-acetyl-galactosaminidase; N-acetylglucosamine-l-phosphotransferase; and palmitoyl-protein thioesterase; and a lysosomal targeting domain that binds an extracellular domain of human cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner, wherein the lysosomal targeting domain is mature human IGF-II or a mutein of mature human IGF-II having an amino acid sequence at least 70% identical to mature human IGF-II.