Patent ID: 8900816

Claim:
A method of determining an amount of EGFRvIII-specific antibody in a blood sample of a human, comprising: contacting a first portion of the blood sample with a peptide immobilized to a solid support, wherein the peptide comprises at least 4 and up to 17 contiguous amino acids of SEQ ID NO: 1 including position 6 of SEQ ID NO: 1; wherein the contacting is performed in the presence of a free form of the peptide of at least 4 and up to 17 contiguous amino acids of SEQ ID NO: 1; contacting a second portion of the blood sample with the peptide immobilized to the solid support, wherein the contacting is performed in the absence of the free form of the peptide; determining a first amount of antibody from the first portion of the blood sample which bound to the peptide immobilized to the solid support; determining a second amount of antibody from the second portion of the blood sample which bound to the peptide immobilized to the solid support; calculating a third amount by subtracting the first amount from the second amount, said third amount representing EGFRvIII-specific antibody in the blood sample of the human.