Patent ID: 8394250

Claim:
A method for electroblotting a sample from an electrophoresis gel to a blotting membrane comprising: providing at least a first and a second pre-packaged ion reservoir, said first ion reservoir comprising a blotting membrane juxtaposed with a matrix having a source of ions sufficient for performing electroblotting, and said second ion reservoir comprising a matrix having a source of ions for performing electrophoresis; forming an electrophoresis transfer stack by juxtaposing an analysis gel with the blotting membrane juxtaposed with the first ion reservoir, and electrically coupling the first ion reservoir to a first electrode and juxtaposing said second ion source with the analysis gel and electrically coupling said second ion source to a second electrode, wherein said analysis gel comprises one or more biomolecules to be transferred to the blotting membrane; and passing a current of at least about 10 mA/cm 2 between the first electrode and the second electrode such that at least a portion of the one or more biomolecules are transferred from the analysis gel to the blotting membrane.