Patent ID: 8080144

Claim:
A method for performing electrophoretic separation from a sample of one or more compounds having distinct electrophoretic mobilities, comprising the steps of: a) providing a separation path, the separation path having an inlet end and an outlet end; b) providing a separation buffer in fluid contact with said outlet end; c) providing a sample in fluid contact with said inlet end of the separation path, the sample containing one or more compounds having distinct electrophoretic mobilities; d) applying an electric field along the length of the separation path to drive electrokinetic motion of the compounds; e) varying with respect to time a bulk fluid flow through the separation path concurrent with the application of the electric field in order to affect whether and when each of the one or more compounds moves into and through the separation path, whereby, when the sample comprises multiple compounds with distinct electrophoretic mobilities, each such compound that moves into the separation path is made to do so at a different time; and wherein the sample is in fluid contact with the inlet end of said separation path continuously throughout the electrophoretic separation of the one or more compounds.