Patent ID: 7396643

Claim:
A screening and/or quantification method of one or more activated transcriptional factors(s) present in a cell or cell lysate, said method comprising the steps of: (a) binding to an insoluble solid support double-stranded DNA sequence(s) at the concentration of at least 0.01 pmole/cm 2 of said solid support surface, wherein the solid support is an array bearing at least 4 spots/cm 2 of solid support surface, each spot containing double-stranded DNA sequence(s) for the binding of activated transcriptional factor(s), said double-stranded DNA sequence comprising a specific sequence, said specific sequence being able to bind said one or more activated transcriptional factor(s) and said double-stranded DNA sequence being connected to the surface of the solid support by a spacer wherein said spacer is a double-stranded DNA nucleotide sequence of between about 50 and about 250 base pairs, or wherein the spacer comprises a double-stranded DNA nucleotide sequence of between about 50 and about 250 base pairs, and wherein said double-stranded DNA nucleotide sequence of between about 50 and about 250 base pairs is not present in said cell; (b) putting into contact said one or more activated transcriptional factor(s) with said bound double-stranded DNA sequence(s); and (c) identifying and/or quantifying a signal specific for the binding of said activated transcriptional factor(s) upon said double-stranded DNA sequence(s).