Patent ID: 8841093

Claim:
A microfluidic device comprising: a microfluidic reagent assembly area for forming two or more sample plugs, said micro fluidic reagent assembly area comprising: i. a microfluidic inline channel, wherein the inline channel is configured to receive a carrier liquid; ii. a sample droplet forming area for forming one or more sample droplets containing genomic material, said sample droplet forming area comprising: an inlet for receiving one or more samples containing genomic material to be formed into said one or more sample droplets, and a genomic material isolation area in fluid communication with the inlet; wherein said genomic material isolation area is in further fluid communication with a sample droplet micro fluidic path; and wherein said sample droplet microfluidic path is in fluid communication with said microfluidic inline channel; iii. an intersection between the sample droplet microfluidic path and the microfluidic in line channel, wherein said sample droplet is combined with carrier liquid to form a sample droplet; iv. a primer plug producing area, said primer plug producing area comprising a primer assembly apparatus, said primer assembly apparatus comprising: a primer plug microfluidic path having a first end and a second end, wherein the primer plug microfluidic path is configured to receive a carrier liquid at the first end; multiple inlets positioned along the length of the primer plug microfluidic path between the first and second ends for receiving amplification reagents and at least one primer in fluid communication with said primer plug microfluidic path; wherein said amplification reagents and at least one primer are combined with carrier liquid to form multiple primer plugs; and wherein the second end of said primer plug micro fluidic path is in fluid communication with said micro fluidic inline channel; and v. a sample plug mixing area located downstream from the intersection between the sample droplet microfluidic path and the microfluidic in line channel, wherein the sample plug mixing area comprises an intersection between said microfluidic in-line channel and the second end of said primer plug microfluidic path, wherein said sample droplet and primer plug are mixed to form the sample plugs; wherein said microfluidic in-line channel passes through an amplification area and a detection area following the micro fluidic reagent assembly area.