Patent ID: 8431405

Claim:
A device for selectively detecting hyperglycosylated human chorionic gonadotropin (hCG-H) in a liquid sample deposited directly or indirectly on a proximal portion of the device for transport to a distal portion of the device, wherein the device comprises: A) a release medium formed of a first material and comprising: i) a labeled conjugate comprising a detectable label and a first binding member that is reactive with a first epitope of hCG-H; and ii) a capturable component comprising a second binding member reactive with a second epitope of hCG-H, at least one of the first binding member and the second binding member exhibiting a moderate to high affinity for the respective epitope of hCG-H and being selectively or preferentially reactive with the respective epitope of hCG-H, such that in the presence of hCG-H in the sample, a complex is formed comprising the labeled conjugate, hCG-H, and the capturable component; and B) a capture medium in fluid communication with the release medium and formed of a second, different material, the capture medium comprising a capture site having immobilized thereon a capture component having a binding affinity for the capturable component.