Patent ID: 8349563

Claim:
A method for nucleic acid amplification, said method comprising: producing an extended primer by performing a reverse transcription reaction on a target nucleic acid sequence using a first primer comprising a first 3′ target specific region and a 5′ universal region, wherein the first 3′ target specific region comprises at least 6 thymines; hybridizing a second primer to the extended primer, wherein the second primer comprises a second 3′ target specific region and the 5′ universal region; extending a nucleic acid sequence from the second primer using the extended primer as the template for the extension, thereby forming a double extended primer; allowing the double extended primer to self-hybridize, wherein the self hybridization occurs via the universal region and a sequence that is complementary to the universal region, thereby forming a looped section between the universal region and the sequence that is complementary to the universal region; adding a third primer that is complementary to an insert section within the double extended target primer; and performing a PCR amplification to amplify the insert section.