Patent ID: 8361715

Claim:
A method for detecting 2 biological events in a reaction medium, comprising employing 2 pairs of fluorescent FRET partner conjuguates, each pair comprising a donor fluorophore and an acceptor fluorophore, λ1 being the excitation wavelength of the donor fluorophores and λ2 being the emission wavelength of the acceptor fluorophores, said method comprising: (i) bringing the reaction medium into contact with a first pair of fluorescent FRET partner conjugates specific for a first biological event; (ii) exciting the reaction medium at the wavelength λ1; (iii) measuring the FRET signal emitted by the reaction medium at the wavelength λ2 and corresponding to the first biological event; (iv) introducing into the reaction medium a FRET signal killer specific for the first pair of fluorescent FRET partner conjugates brought into contact in step (i); (v) bringing the reaction medium into contact with a pair of fluorescent FRET partner conjugates specific for a second biological event different from the first biological event detected in the preceding steps; (vi) exciting the reaction medium at the wavelength λ1; (vii) measuring the FRET signal emitted by the reaction medium at the wavelength λ2, corresponding to the second biological event for which the pair of fluorescent FRET partner conjugates brought into contact in step (v) is specific.