Patent ID: 8906641

Claim:
A method for specifically measuring α-amylase contained in a non-human animal sample comprising α-amylase and glucoamylase without diluting said sample, which method comprises a reaction of decomposing an oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof by the α-amylase in said sample by applying an undiluted non-human animal sample to a dry analysis element comprising the oligosaccharide and a mordant on an identical or different reagent layer thereof; and a reaction of decomposing the thus obtained product by α-glucosidase, wherein pH of the reactions is 6 to 6.5; the non-human animal sample is a canine sample or a cat sample, and the oligosaccharide having a protected nonreducing terminus and a p-nitrophenyl group at the reducing terminus thereof is 4,6-ethylidene-4-nitrophenyl-α-D-maltoheptaoside, and the α-amylase activity is calculated from the time of 1 to 2 minutes after the application of the undiluted non-human animal sample to the dry analysis element to the time of 2 to 4 minutes after said application.