Patent ID: 6921652

Claim:
A process for hydrolyzing an oil or fat comprising: i) mixing and feeding an oil-phase substrate and a water-phase substrate to a packed layer comprising an enzyme immobilized on a carrier packed therein; and ii) determining a maximum pressure loss ΔP [MPa] through said packed layer during said hydrolytic reaction, a thickness L [m] of said packed layer, a weight-basis average particle size dp [m] of particles of said packed enzyme immobilized on a carrier, and a void volume ε[−] of said packed layer, wherein said feeding is under conditions such that a shear stress factor (τw) is within a range of from 1×10 −4 to 1.4×10 −3 MPa, which is applied to a surface of said enzyme immobilized on a carrier and is expressed by the following formula (1): τw= (Δ P/L )× dp×ε/ (1−ε) (1) wherein ΔP,L, dp and ε are as defined above.