Patent ID: 7879572

Claim:
A method of measuring the activity of a compound as an enterostatin agonist or antagonist, said method comprising the sequential steps of: a. Contacting an in vitro mammalian cell with the compound in the presence of enterostatin under conditions wherein enterostatin will increase the amount of F 1 -ATPase β-subunit in the plasma membrane of the cell to a known level, and b. Measuring the resulting concentration of F 1 -ATPase β-subunit in the plasma membrane, Wherein an increased level of F 1 -ATPase β-subunit in the plasma membrane above the known level due to enterostatin alone indicates that the compound possesses activity as an enterostatin agonist and wherein a decreased level of F 1 -ATPase β-subunit in the plasma membrane below the known level due to enterostatin alone indicates that the compound possesses activity as an enterostatin antagonist.