Patent ID: 8236949

Claim:
A method of performing a coupling reaction, comprising contacting a 1,2,4,5-tetrazine with a dienophile in an organic or aqueous medium comprising at least one species selected from the group consisting of primary amines, thiols, Staudinger reactants, Staudinger adducts, Sharpless-Huisgen reactants, and Sharpless-Huisgen adducts; said contacting performed under conditions sufficient to convert at least 50% of the 1,2,4,5-tetrazine or the dienophile to a Diels-Alder adduct of the 1,2,4,5-tetrazine with the dienophile; wherein the dienophile is selected from the group consisting of trans-cyclooctene, substituted trans-cyclooctenes and compounds according to formulas (7), (8) and (27)