Patent ID: 8678686

Claim:
A composition which is useful for introducing a biologically active agent into a cell as a transfection agent, comprising: a lipopolyamine comprising: Wherein A N) n is linear, branched or dendritic polyamine, wherein n=3-100, wherein n is the number of amino groups in the polyamine; R 1 is independently H, or a substitute of structure (X—Y—Z) wherein X=(CH 2 ) i , wherein i=1-12; Y=—C(O)NH—, —NHC(O)—, —CH 2 —, —O—, —C(O)O—, or —C(O)—; Z=linear or branched alkyl chain or alkenyl chain having 4-40 carbon atoms in length; wherein the chain has zero to six double bonds; R 2 is a substitute of structure of (X—Y—Z) m , wherein X=—(CH 2 ) i —, wherein i=1-12, Y=—C(O)NH—, —NHC(O)—, —CH 2 —, —O—, —C(O)O—, or —C(O)—; Z=linear or branched alkyl chain or alkenyl chain having 4-40 carbon atoms in length; wherein the chain has zero to six double bonds; m is at least 1; and wherein the lipopolyamine is mixed with one or more helper lipids which elevates transfection activity; and further comprising one or more biologically active agents; and one or more pharmaceutically acceptable solvents, carriers and/or additives; and wherein the composition is an aqueous liposome formulation.