Patent ID: 6955879

Claim:
A method for producing recombinant polynucleotides comprising the steps of: (a) generating a pool of unidirectional single-stranded polynucleotide fragments randomized in length from at least one starting polynucleotide to be reassembled, which have regions of similarity with each other; (b) conducting a polymerization process comprising multi-cyclic extension reactions, wherein the unidirectional single-stranded polynucleotide fragments prepared by step (a) serve as templates sequentially and specific oligonucleotides are added to the reaction mixture as primers, the primers being extended by means of template switching to produce at least one recombinant polynucleotide, and the resulting recombinant polynucleotides being different from the starting polynucleotides in nucleotide sequence; and (c) conducting a polymerase chain reaction using at least one specific primer to amplify the recombinant polynucleotides prepared by step (b).