Patent ID: 7955563

Claim:
A method for applying reactive films containing solids to microporous, absorbent, blood-separating membranes comprising: providing a microporous membrane, said membrane capable of generating plasma or serum from whole blood; providing a water-resistant and/or water-insoluble reactive film containing solids, the reactive film further containing film formers and film openers, wherein said film openers are inorganic or organic particulate fillers, the amount of filler being greater than the amount of film former, said reactive film further containing at least portions of a detection reagent for an analyte, said detection reagent being provided in a hydrophobic matrix of film formers; moistening the microporous membrane with water or an aqueous solution; and applying the reactive film to the microporous membrane while said membrane is still moist, thereby providing an article comprising a microporous membrane layer and a water-resistant and/or water-insoluble reactive film layer, said microporous membrane layer and said reactive film layer being different layers.