Patent ID: 7655467

Claim:
A method of systemic transduction of skeletal muscle tissue or heart tissue comprising; a) providing; i) a first composition comprising adeno-associated vectors, wherein said adeno-associated vectors comprise a nucleic acid sequence of interest, ii) a second composition comprising a systemic transduction enhancing agent, wherein said systemic transduction enhancing agent comprises empty adeno-associated viral capsids, and wherein at least 50% of the adeno-associated viral capsids in said second composition are said empty adeno-associated viral capsids, and iii) a subject comprising a first type of extravascular tissue, wherein said first type of extravascular tissue is skeletal muscle tissue or heart tissue; and b) administering said first and second compositions systemically to said subject, under conditions such that said first type of extravascular tissue is transduced by said adeno-associated vectors, wherein said first and second compositions are: i) administered simultaneously; ii) are administered within 5 minutes of each other, or iii) mixed together prior to said administering.