Patent ID: 8227185

Claim:
A method of diagnosing in vitro an HIV-1 virus of the non-M, non-O group by means of hybridization and/or gene amplification, which method is carried out using a biological sample (serum or circulating lymphocyte) and comprises: a step of extracting a nucleic acid which is to be detected and which belongs to the genome of the virus, which virus may possibly be present in the biological sample, and, optionally, a step of treating the nucleic acid using a reverse transcriptase, if this nucleic acid is in RNA form; at least one cycle comprising the steps of denaturing the nucleic acid, of hybridizing with at least one hybridizing nucleic acid selected from the group consisting of SEQ ID NO: 17 or 44 to 52 and, optionally, extending the hybrid, which has been formed, in the presence of DNA polymerase and dNTP, and a step of detecting the possible presence of the nucleic acid belonging to the genome of a virus of the non-M, non-O HIV-1 group type.