Patent ID: 8030270

Claim:
A method for identifying a Glucagon-like Peptide-1 (GLP-1) secretagogue, comprising: (a) contacting a G protein-coupled receptor (GPCR) with a ligand to the GPCR in the presence of a test compound, wherein the GPCR comprises an amino acid sequence selected from the group consisting of: (i) SEQ ID NO: 2; and (ii) the amino acid sequence of a GPCR encoded by the nucleotide sequence set forth in SEQ ID NO: 1; (b) detecting a complex between the GPCR and the ligand; (c) determining whether less of the complex is formed in the presence of the test compound than in the absence of the test compound; (d) determining the blood GLP-1 level in a blood or a plasma sample obtained from a mammal, the mammal having been administered with the test compound in the presence of which less of the complex is formed in step (c), wherein the ability of the test compound to increase a blood GLP-1 level in the blood or the plasma sample obtained from the mammal identifies the test compound as a GLP-1 secretagogue.