Patent ID: 8759609

Claim:
A method for increasing the transformation efficiency of a maize transformation process comprising limiting the anaerobiosis effect during the inoculation of Agrobacterium to the transformable maize tissue explant by (i) isolating a maize explant directly into inoculation medium containing Agrobacterium and removing the maize explant from the inoculation medium within 20 minutes; (ii) spotting a maize explant with an Agrobacterium solution wherein the maize explant is contacted by the Agrobacterium solution for 5 to 60 minutes; or (iii) contacting the maize explant with filter paper comprising a solution of Agrobacterium for 5 to 60 minutes; thereby obtaining transformed maize tissue with an increased transformation efficiency; wherein the transformed maize tissue is cultured in a selection medium at a temperature of from 30° C. to about 34° C. during the first two weeks of selection, and then cultured in a selection medium at a temperature of about 27° C. during the final week of selection.