Patent ID: 8753840

Claim:
A purified genetically engineered Synechocystis sp. PCC 6803 cyanobacterium, wherein the engineered cyanobacterium has been transformed with a plasmid vector comprising a first nucleotide sequence encoding acetyl-CoA carboxylase A (accA) (sll0728), acetyl-CoA carboxylase B (accB) (slr0435), acetyl-CoA carboxylase C (accC) (slr0053), and acetyl-CoA carboxylase D (accD) (sll0336), and a second nucleotide sequence encoding vesicle-inducing protein in plastids 1 (VIPP1) (sll0617) constitutively linked to a psbA3 promoter, wherein the first nucleotide sequence replaces the endogenous psbA2 gene of the said host genome and the second nucleotide sequence replaces the endogenous psbA3 gene of the said host genome wherein the plasmid is designed for insertion into Synechocystis genome psbA2 locus and wherein the purified genetically engineered Synechocystis sp. PPC 6803 cyanobacterium has an increased expression of Synechocystis sp. PCC 6803 acetyl-CoA (ACC) and an increased production of fatty acids or lipids as compared to a native Synechocystis sp. PCC 6803 cyanobacterium, and wherein the increased production in fatty acids or lipids content is at least 47% of the said purified genetically engineered Synechocystis sp. PPC 6803 cyanobacterium's dry weight.