Patent ID: 6967074

Claim:
A method of measuring enzyme activity, comprising: providing a base plate having a mating side; treating the base plate with a solution of mixed self-assembled monolayer (SAM) comprising about 0.1 to 20% maleimide-terminal groups in a background of tri(ethylene glycol) terminal groups: providing a removable member having a first sealable surface and a second sealable surface and defining at least one orifice extending between the first sealable surface and the second sealable surface; reversibly sealing the first sealable surface of the removable member to the mating side of the base plate; adding at least one biological material to the at least one orifice for immobilizing the at least one biological material on the base plate; exposing the at least one biological material to at least one reagent via the at least one orifice wherein the reagent contains an enzyme; and measuring a level of activity of the at least one reagent by measuring changes in the at least one biological material.