Patent ID: 7713528

Claim:
A method for visualizing specific target analytes in a subject which comprises: (1) providing a reagent conjugate useful for delivering active compounds to specific target analytes into a subject, said conjugate comprising (a) a first reagent comprising a specific target recognition component which comprises a Fab′ antibody fragment which is covalently attached to a first sequence-specific component comprising a first oligonucleotide comprised of 2′-chlorodeoxy-guanosine which is about 20 bases long; and (b) a second reagent comprising an active compound component linked to a second sequence-specific component which specifically recognizes and binds to said first sequence-specific component, said second reagent-comprised of 90 y bound to a 1,2-diaminocyclohexane-tetraacetate (DCTA) chelating moiety, said second sequence-specific component comprising a second oligonucleotide, said DCTA chelating moiety being covalently attached through the allylamino group at the 5-position of a cytidyl residue in said second oligonucleotide which comprises the sequence [(2′-chloro-deoxycytosine) 3 (2′-chloro-deoxycytosine-5-allylamine)] 5 , and wherein said first and second sequence-specific components (a) and (b) have been rendered nuclease resistant by the presence of one or more nucleotide analogs to said oligonucleotides; (2) introducing into said subject an effective amount of said reagent conjugate; and (3) visualizing said specific target analytes.