Patent ID: 8557743

Claim:
A method of selecting phage that encode a target binding protein from a plurality of display phage, the method comprising: a) forming a mixture comprising a plurality of diverse display phage, a target, and a support, wherein each phage of the plurality displays a heterologous protein component on its surface and each phage includes a nucleic acid encoding the heterologous protein component, the heterologous protein component being a member of a set of diverse protein components; b) forming phage immobilized to the support comprising binding a phage from the plurality to the target and immobilizing the target to the support; c) separating phage that do not bind to the target from the phage immobilized to the support via binding to the target; d) contacting host cells with the phage immobilized to the support so that the host cells are infected by the phage immobilized to the support to yield a first population of infected cells; e) producing replicate phage from the infected cells in the presence of the target immobilized to the support, thereby forming replicate phage immobilized to the support via binding to the target of step (a) without first purifying or harvesting the replicate phage, wherein the producing is completed in less than 2 hours; f) separating replicate phage that do not bind to the target of step (a) from the replicate phage immobilized to the support; and g) contacting host cells with the replicate phage immobilized to the support so that host cells are infected with the replicate phage immobilized to the support to yield a second population of infected cells.