Patent ID: 7932045

Claim:
A method of isolating CMV peptide-specific CD8+ T cells using anti-4-1BB antibodies, said method comprising the steps of: 1) isolating peripheral blood mononuclear cells (PMBCs) from blood and culturing the cells in culture media with CMV peptide; 2) isolating PMBCs from the same blood as in the previous step, culturing the cells with OKT3 antibody, and washing and labeling the cells with CMV peptide; 3) culturing a cell mixture consisting of the cultured cells in step 1) and the labeled cultured cells in step 2) in a range of ratios from 1:1 to 10:1 for 1 to 3 days; 4) culturing the cell mixture in step 3) in an anti-4-1BB mAb coated culture plate and removing unattached or non-specifically bound cells; and 5) culturing attached cells in step 4) and harvesting all of the cells.