Patent ID: 7087395

Claim:
A kit for determining a concentration of 25-hydroxy vitamin D (25-OH-D) in a sample, comprising: a releasing composition including from about 0.01 to about 5% of a cyclodextrin, from about 0.01 to about 5% of a sodium salicylate, and from about 0.1 to about 1.0 M NaOH, the cyclodextrin, the salicylate, and the NaOH being provided in an amount effective to reduce interference from a protein or a lipid with 25-hydroxy vitamin D present in a sample; 25-hydroxy vitamin D coupled to a solid phase; vitamin D binding protein; and a vitamin D binding protein antibody coupled to a label present in an amount that produces a detectable signal when 25-hydroxy vitamin D is present in the sample.