Patent ID: 8802376

Claim:
A method of selecting an antibody molecule as a candidate for having cytotoxic activity against a cell which expresses podocalyxin-like protein (PODXL), or is an undifferentiated pluripotent stem cell, wherein the antibody molecule binds PODXL, the method comprising the steps of: (i) comparing the binding of a PODXL-binding antibody molecule to a glycan comprising Fucα1-2Galβ1-3GlcNAc with the binding of a non-cytotoxic PODXL-binding antibody molecule to a glycan comprising Fucα1-2Galβ1-3GlcNAc; and (ii) selecting as a candidate cytotoxic antibody molecule a said PODXL-binding antibody molecule that has a relative binding to said glycan of 30 or more times greater than said non-cytotoxic PODXL-binding antibody molecule.