Patent ID: 8268787

Claim:
A method for enhancing cellular cholesterol efflux in a patient, comprising administering to the patient a composition comprising a particle derivative of at least one form of high density lipoprotein, wherein the particle derivative comprises a protein shell and lipid and is obtained by a process comprising the steps of: a. connecting a patient to a device for withdrawing blood; b. withdrawing blood containing blood cells from the patient; c. separating the blood cells from the blood to yield a blood fraction containing high density lipoprotein and low density lipoprotein; d. separating the low density lipoprotein from the blood fraction; e. mixing the blood fraction with a solvent which removes lipid associated with the high density lipoprotein to yield a mixture of lipid, the solvent, and the particle derivative; and, f. separating the particle derivative from the lipid and the solvent, wherein the particle derivative comprises apolipoprotein A1 and phospholipid, and wherein the particle derivative has a reduced lipid content as compared to the high density lipoprotein particle that does not have the solvent treatment.