Patent ID: 7314632

Claim:
A non-toxic Pseudomonas exotoxin A-like chimeric immunogen comprising in sequence: (1) a cell recognition domain comprising domain 1a of Pseudomonas exotoxin A (PE); (2) a translocation domain comprising a translocation domain of PE wherein the translocation domain is at least 95% identical to SEQ ID NO:2 from amino acid position 280 to amino acid position 344 and serves to translocate the instant chimeric immunogen to the cytosol of the cell; (3) an epitope presenting domain of between 5 and 350 amino acids in length that comprises one cysteine-cysteine loop wherein the loop encodes an epitope of a pathogen and wherein the epitope is non-native to PE domain Ib; (4) an endoplasmic reticulum (ER) retention domain wherein the ER domain is capable of effecting translocation to the endoplasmic reticulum of the cell and wherein the ER retention domain lacks ADP ribosylation activity.