Patent ID: 7037661

Claim:
A composition for use in labeling one or more nucleic acid molecules, said composition comprising: a) one or more enzymes having reverse transcriptase activity, wherein said enzymes are multi-subunit enzymes and are reduced or substantially reduced in RNase H activity; and b) one or more deoxynucleotides labeled with a fluorescent label selected from the group consisting of 5-carboxyfluorescein (FAM), 2′7′-dimethoxy-4′5-dichloro-6-carboxyfluorescein (JOE), 6-carboxyrhodamine (R6G), N,N,N′,N′-tetramethyl-6-carboxyrhodamine (TAMRA), 6-carboxy-X-rhodamine (ROX), 4-(4′dimethylaminophenylazo) benzoic acid (DABCYL), Cascade Blue, Oregon Green, Cyanine, 5-(2′-aminoethyl)aminonaphthalene-1-sulfonic acid (EDANS), Cy3, Cy5, ChromaTide, BODIPY-FL and BODIPY-TMR.