Patent ID: 7300759

Claim:
A method for identifying nucleic acids encoding proteins which bind a mouse tramd 1 protein encoded by a nucleic acid selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 13, SEQ ID NO: 45 and SEQ ID NO: 46, said method comprising: (a) providing: (i) a first recombinant vector comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 13, SEQ ID NO: 45, and SEQ ID NO: 46 in operable combination with the DNA binding domain of a transcriptional activator, such that a chimeric protein will be expressed, (ii) a population of second recombinant vectors, wherein said population comprises a library of cDNA molecules in operable combination with the activation domain of a transcriptional activator, such that a population of chimeric proteins will be expressed, and (iii) a yeast host comprising a reporter gene in operable combination with the DNA binding sites for said transcriptional activator; (b) introducing said first vector and said population of second vectors into said yeast host to generate a population of transformed yeast; (c) subjecting said population of transformed yeast to conditions such that said chimeric proteins are expressed; (d) screening said population for members which express said reporter gene; and (e) isolating the cDNA molecule from said second vector from members of the population which express the reporter gene, thereby identifying nucleic acids encoding proteins which bind a mouse tramd 1 protein.