Patent ID: 7262040

Claim:
A method for synthesis of a glycoprotein, the method comprising incorporating into a protein an unnatural amino acid that comprises a saccharide moiety, wherein the incorporating step comprises using an orthogonal tRNA/orthogonal aminoacyl-tRNA synthetase (O-tRNA/O-RS) pair, wherein the O-tRNA recognizes a selector codon and incorporates the unnatural amino acid into the protein in response to the selector codon, and wherein the O-RS preferentially aminoacylates the O-tRNA with the unnatural amino acid, wherein the method further comprises contacting the saccharide moiety with a recombinant glycosyltransferase, a sugar donor moiety, and other reactants required for glycosyltransferase activity for a sufficient time and under appropriate conditions to transfer a sugar from the sugar donor moiety to the saccharide moiety.