Patent ID: 8097421

Claim:
A method of conducting a multiplex immunoassay comprising: providing a substrate and forming a plurality of different complexes associated with a surface of the substrate, wherein the plurality of different complexes comprises a plurality of first binding partners immobilized on the surface of the substrate, a plurality of different analytes bound to the plurality of first binding partners, a plurality of second binding partners bound to the plurality of different analytes and a plurality of different labels bound to the plurality of second binding partners, wherein each of the plurality of different complexes comprises at least one binding partner comprising an antibody; disassociating the plurality of different labels from the surface of the substrate; flowing said plurality of disassociated labels through a detection device, wherein said detection device comprises at least one microfluidic channel having a probe volume adapted to contain no more than one label at any given time, and detecting the plurality of labels on an individual basis by said detection device, wherein the substrate comprises (a) an integrated circuit, and (b) a multi-well plate or an array, wherein the array comprises a microarray or a macroarray, further wherein the integrated circuit and the multi-well plate are integrally integrated within the substrate, or the integrated circuit and the array are integrally integrated within the substrate.