Patent ID: 7704709

Claim:
A modified human granulocyte-colony stimulating factor(hG-CSF) which is different from a parent wild-type hG-CSF of the amino acid sequence of SEQ ID NO: 2 by having at least one replacements of amino acid residues at the positions 1st, 2nd, 3rd and 17th amino acids of the wild-type hG-CSF, wherein the replaced amino acids in the modified hG-CSF are different from one present in the parent wild-type hG-CSF; wherein the modified hG-CSF has no sugar chain; wherein the modified hG-CSF has no terminal Met residue at the N-terminus thereof; wherein when the 1st amino acid is replaced, the first amino acid in the modified hG-CSF is Ser; and when the 17th amino acid is replaced, the 17th amino acid in the modified hG-CSF is an amino acid which is not charged at neutral pH.