Patent ID: 8741261

Claim:
A method of using produced carbon-isotope enriched gas mixture in labeling synthesis, comprising: (a) providing a high pressure reaction chamber having a liquid inlet and a gas inlet in a bottom surface thereof, (b) providing an azide solution to be labeled comprising an azide of the formula R 1 —N 3 , wherein R 1 , is linear or cyclic lower alkyl or substituted alkyl, hydrogen, aryl or substituted aryl and optionally comprises carbonyl, hydroxy, thiol, halogen, nitrile, isonitrile, cyanate, isocyanate, thiocyanate, isothiocyanate functional groups, a transitional metal complex and a reagent, wherein the reagent is: R 2 , R 3 , R 4 , and R 5 are independently linear or cyclic lower alkyl or substituted alkyl, hydrogen, aryl, or substituted aryl, and optionally comprise carbonyl, hydroxy, thiol, halogen, nitrile, isonitrile, cyanate, isocyanate, thiocyanate, isothiocyanate functional groups, (c) introducing a carbon-isotope monoxide enriched gas-mixture into the reaction chamber via the gas inlet, (d) introducing, at high pressure, said azide solution comprising said transition metal complex and one of said reagents into the reaction chamber via the liquid inlet, (e) waiting for a predetermined time while said labeling synthesis occurs, and (f) collecting the labeled compound from the reaction chamber, wherein the labeled compound is: wherein R 1 , is linear or cyclic lower alkyl or substituted alkyl, hydrogen, aryl or substituted aryl and optionally comprises carbonyl, hydroxy, thiol, halogen, nitrile, isonitrile, cyanate, isocyanate, thiocyanate, isothiocyanate functional groups and R 2 , R 3 , R 4 and R 5 are independently linear or cyclic lower alkyl or substituted alkyl, hydrogen, aryl or substituted aryl, and optionally comprise carbonyl, hydroxy, thiol, halogen, nitrile, isonitrile, cyanate, isocyanate, thiocyanate, isothiocyanate functional groups, and * is the labeled carbon position.