Patent ID: 7081527

Claim:
A set of amplification oligonucleotides for use in amplifying Cryptosporidium parvum nucleic acid, said set of amplification oligonucleotides comprising: a first amplification oligonucleotide, wherein the base sequence of said first amplification oligonucleotide consists of a base sequence selected from the group consisting of SEQ ID NO:46, SEQ ID NO:52, SEQ ID NO:58 and SEQ ID NO:64 and, optionally, a 5′ sequence that is recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase; and a second amplification oligonucleotide, wherein the base sequence of said second amplification oligonucleotide consists of a base sequence selected from the group consisting of SEQ ID NO:47, SEQ ID NO:53, SEQ ID NO:59 and SEQ ID NO:65 and, optionally, a 5′ sequence that is recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase, wherein said amplification oligonucleotides are capable of amplifying a target sequence present in a target nucleic acid derived from Cryptosporidium parvum under amplification conditions.