Patent ID: 8338116

Claim:
A method to test the effect of a stimulatory event on a cell comprising: providing a RWG (Resonant Wave Guide Grating) label-free biosensor system including a light source, and a label independent detector; incubating a cell on the biosensor, wherein the cell has a labeled molecule that is part of a signaling cascade; providing a stimulatory event to the incubated cell; collecting from within the cell, at the cell surface, or a combination thereof, the DMR (Directional Mass Redistribution) biosensor output from the biosensor; analyzing the DMR to determine the effect of the stimulatory event on the cell; detecting a change in the labeled molecule with a label detector; and dissecting the biological pathway of the stimulatory event induced DMR by the detected change in the labeled molecule, wherein the change in the labeled molecule is the concentration of the labeled molecule, the location of the labeled molecule, or a combination thereof.