Patent ID: 8222012

Claim:
A process for producing a target concentration of peroxycarboxylic acid comprising: (a) selecting a set of reaction components comprising: (1) at least one substrate selected from the group consisting of: (i) esters having the structure [X] m R 5 wherein X=an ester group of the formula R 5 —C(O)O; R 6 ═C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R 6 optionally comprises one or more ether linkages for R 6 ═C2 to C7; R 5 =a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety optionally substituted with hydroxyl groups; wherein each carbon atom in R 5 individually comprises no more than one hydroxyl group or no more than one ester group; wherein R 5 optionally comprises one or more ether linkages; m=1 to the number of carbon atoms in R 5 ; and wherein said esters have solubility in water of at least 5 ppm at 25° C.; (ii) glycerides having the structure wherein R 1 ═C 1 to C 7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R 3 and R 4 are individually H or R 1 C(O); and (iii) acetylated saccharides selected from the group consisting of acetylated monosaccharides, acetylated disaccharides, and acetylated polysaccharides; (2) a source of peroxygen; and (3) an enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme having a signature motif that aligns with a reference sequence SEQ ID NO:2 using CLUSTALW, said signature motif comprising: (i) an RGQ motif at amino acid positions 118-120 of SEQ ID NO:2; (ii) a GXSQG motif at amino acid positions 179-183 of SEQ ID NO:2; and (iii) an HE motif at amino acid positions 298-299 of SEQ ID NO:2; wherein said enzyme has at least 95% amino acid identity to SEQ ID NO:4; and (b) combining the reaction components under aqueous reactions to form a reaction mixture; whereby reaction products are formed comprising enzymatically-produced peroxycarboxylic acid; wherein (1) the pH of the reaction mixture remains in the range of from about 6.0 to about 9.0; and (2) the concentration of peroxycarboxylic acid produced one minute after combining the reaction components is not exceeded by more than 100% at a reaction time equal to or greater than five minutes after combining the reaction components.