Patent ID: 7264958

Claim:
A method for purifying infectious adenoviral particles from a crude viral preparation containing said adenoviral particles, comprising: i) a fluidized bed chromatography step comprising at least the steps of: a. contacting said crude viral preparation with particles of adsorbent in fluidized bed under suitable conditions to allow adsorption of said adenoviral particles onto said particles of adsorbent, b. eluting the adsorbed adenoviral particles from said particles of adsorbent; and c. collecting the eluted adenoviral particles; wherein said particles of adsorbent comprise an agarose matrix and a central core comprising quartz, and dextran chains covalently coupled to said agarose matrix, on which are attached positively charged groups; and, ii) a gel filtration chromatography step that is carried out on a support comprising an alkyl dextran and methylene bisacrylamide matrix or an ethylene glycol and methacrylate matrix.