Patent ID: 8153962

Claim:
A method for determining the amount of one or more analytes in a sample by mass spectrometry, the one or more analytes selected from the group consisting of pregnenolone and 17-hydroxypregnenolone (17-OH pregnenolone), the method comprising: a. subjecting a single sample injection to an ionization source under conditions suitable to ionize said one or more analytes from said sample to generate one or more ions detectable by mass spectrometry from each of said one or more analytes wherein the one or more analytes are not derivatized prior to ionization; b. determining the amounts of one or more ions from each of said one or more analytes by tandem mass spectrometry; and c. using the amounts of one or more ions from each of said one or more analytes to determine the amount of each of said one or more analytes in the sample.