Patent ID: 7575908

Claim:
A method for immobilization of an alpha one (α 1 ) acid glycoprotein, comprising: activating an affinity support, the affinity support being activated by reacting the affinity support with a compound that is reactive with one or more functional groups including within the one (α 1 ) acid glycoprotein; oxidizing the alpha one (α 1 ) acid glycoprotein, oxidation conditions being selected to i) yield an oxidized alpha one (α 1 ) acid glycoprotein that includes at least five reactive aldehyde groups and ii) maximize activity of said alpha one (α 1 ) acid glycoprotein after oxidation, wherein said oxidation conditions comprise reacting the alpha one (α 1 ) acid glycoprotein with 5 mM to 20 mM periodate for about 10 to 15 minutes; reacting the oxidized alpha one (α 1 ) acid glycoprotein with the activated affinity support to immobilize the alpha one (α 1 ) acid glycoprotein by linking the oxidized alpha one (α 1 ) acid glycoprotein to the activated affinity support.