Patent ID: 8753834

Claim:
A method of detecting the presence or absence of a microorganism in a sample, comprising: providing, a liquid sample suspected of containing a microorganism; a detection article comprising: a base member comprising a first dry coating that includes a cold water-soluble gelling agent; a microporous membrane; a cover sheet comprising a second dry coating that includes a first detection reagent; a barrier layer that forms a fluid barrier between the microporous membrane and the cover sheet; wherein the microporous membrane is disposed between the base member and the barrier layer; contacting a predetermined amount of the sample with the first dry coating; contacting the microporous membrane with the sample; incubating the article for a first period of time; repositioning the barrier layer to provide contact between the cover sheet and the microporous membrane; incubating the article for a second period of time; and observing an indication of the presence or absence of microbial growth.