Patent ID: 8501438

Claim:
A method of producing a protein having reduced O-linked glycosylation comprising: (a) providing Pichia pastoris or Saccharomyces cerevisiae host cells comprising a nucleic acid encoding a protein operably linked to an inducible promoter; (b) growing the host cells for a time sufficient to provide a multiplicity of the host cells before contacting the host cells with one or more inhibitors of Pmt-mediated O-glycosylation; (c) contacting the host cells with an inducer of the promoter to induce expression of the protein at a time before or at the same time as contacting the host cells with the one or more inhibitors of Pmt-mediated O-linked glycosylation; and (d) isolating the protein produced by the host cells in the presence of the one or more inhibitors to produce the protein having reduced O-linked glycosylation.