Patent ID: 8759112

Claim:
System for the luminescence detection of an analyte in a liquid sample comprising: (a) a support including an analyte-specific substance which is able to emit a first luminescence signal on contact with the analyte, a reference substance which is able to emit a second luminescence signal which is substantially quenched by contact with the liquid sample, wherein the analyte-specific substance and the reference substance are excitable at essentially the same wavelengths and emit at essentially the same wavelengths, and wherein the analyte-specific substance and the reference substance are fluorophores, and the reference substance is wherein one of R 2 , R 3 and R 5 is selected from the group consisting of NR 11 R 12 and SO 3 Na; R 11 and R 12 are selected from the group consisting of hydrogen and C 1 -C 4 alkyl; and R 1 , R 4 , R 6 , R 7 , R 8 , R 9 and R 10 are selected from the group consisting of hydrogen and a halogen; and (b) a single detector which is adapted to measure the luminescence signals of the analyte-specific substance and of the reference substance.