Patent ID: 8637658

Claim:
A composition useful for synthesizing a quencher-labeled polynucleotide which has the structure (II): wherein: Q is a quencher moiety Q1 having the following structure: wherein: R 1 , R 2 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 and R 14 are each, independently of one another, selected from hydrogen, an electron-withdrawing group and an electron-donating group, or, alternatively R 4 may be taken together with R 5 or R 6 may be taken together with R 7 to form a benzo group, further wherein: at least one of R 1 , R 2 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 or R 14 is an electron-withdrawing group; and at least one of R 1 , R 2 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 or R 14 is an electron-donating group; and a reactive linking group linking the aryl carbon of the quencher moiety to L 1 , either directly or by way of a linker NR″R″ moiety, where each R″ is independently C 1 -C 12 alkyl; L 1 , L 3 and L 4 are each, independently of one another, selected from a bond, C 1 -C 12 alkyldiyl, C 1 -C 12 alkoxyldiyl, C 1 -C 12 alkylaminodiyl, C 1 -C 12 alkylamidediyl, C 5 -C 14 aryldiyl, and 1-20 ethyleneoxy units; L 2 is a C 1 -C 12 alkoxyldiyl, wherein the hydroxyl group is attached to X; A is a cleavable linker; X is an acid-labile protecting group; Y is selected from N and CR, where R is selected from H, C 1 -C 6 alkyl, and C 5 -C 14 aryl; and represents the solid support.