Patent ID: 7655445

Claim:
A process for the synthesis of an epimerically enriched form of a sulfated heparosan polysaccharide, comprising contacting an acceptor heparosan polysaccharide with the following in a mixture: (i) a 3′-phosphoadenosine 5′-phosphosulfate (PAPS) sulfate donor; (ii) at least one sulfotransferase; (iii) a p-nitrophenyl sulfate sulfate donor; (iv) an aryl sulfatase; and (v) an epimerase; wherein: (a) said at least one sulfotransferase catalyzes sulfate transfer from said PAPS sulfate donor to said acceptor heparosan polysaccharide; (b) said PAPS, by donating said sulfate group is converted to a 3′,5′-diphosphoadenosine (PAP); (c) said aryl sulfatase catalyzes sulfate transfer from said p-nitrophenyl sulfate sulfate donor to said PAP, thereby regenerating PAPS; and (d) said epimerase catalyzes conversion of said acceptor heparosan polysaccharide to its epimer; thereby synthesizing an epimerically enriched form of a sulfated heparosan polysaccharide in which the uronic acid residues are not sulfated.