Patent ID: 6992819

Claim:
A method of conducting an agglutination test, comprising: gathering a sample containing at least one analyte; introducing a quantity of carriers into said sample, each of said carriers configured to attach to said at least one analyte; and illuminating at least one binding event with scattered light from a combined light source, said binding event occurring between said carrier and said at least one analyte, said step of illuminating comprising: illuminating a first light source to emit a first light wave traveling at a first frequency; illuminating a second light source to emit a second light wave traveling at a second frequency; positioning an optical combiner to receive and combine said first and second light waves into a combined light, said combined light comprising an additive light wave traveling at an additive frequency and a subtractive light wave traveling at a subtractive frequency; positioning a darkfield condenser to receive said combined light; focusing said combined light upon said binding event such that said additive and subtractive light waves provoke scattered light; and arranging an optical microscope having at least one objective lens and at least one eyepiece to observe said binding event.