Patent ID: 7445895

Claim:
A method for detecting drug-resistant Mycobacterium tuberculosis DNA comprising: (a) amplifying suspected drug-resistant Mycobacterium tuberculosis cDNA with bioactive primers; (b) hybridizing suspected drug-resistant Mycobacterium tuberculosis cDNA with drug-resistant Mycobacterium tuberculosis -specific probes in hybridization tubes; (c) adding blocking solution into the hybridization tubes; (d) adding avidin enzyme complex or streptavidin enzyme complex into the hybridization tubes; (e) performing washing reaction to remove interfering material; (f) adding substrate of enzyme; and (g) detecting the luminescent or color change after adding the substrate of enzyme; and (h) comparing the luminescence or color change in step (g) to the luminescence or color change of a control sample comprising drug-sensitive Mycobacterium tuberculosis DNA, wherein the probes are P1, P2, and P5, P1 is selected from the group consisting of: 5′-CAGCCAGCTGAGCCAATTCAT-3′, (SEQ ID NO:1) 5′-CAGCCAGCTGAGCCAATTCATGGAC-3′, (SEQ ID NO:2) 5′-CAGCCAGCTGAGCCAATTCATGGA-3′, (SEQ ID NO:3) 5′-CAGCCAGCTGAGCCAATTCATGG-3′, (SEQ ID NO:4) 5′-CAGCCAGCTGAGCCAATTCATG-3′, (SEQ ID NO:5) 5′-CAGCCAGCTGAGCCAATTC-3′, (SEQ ID NO:6) 5′-CAGCCAGCTGAGCCAATTCA-3′, (SEQ ID NO:7) 5′-AGCCAGCTGAGCCAATTCATGG-3′, (SEQ ID NO:8) 5′-GCCAGCTGAGCCAATTCATGGA-3′, (SEQ ID NO:9) and 5′-GCCAGCTGAGCCAATTCCATG-3′, (SEQ ID NO:10) P2 is selected from the group consisting of: 5′-TTCATGGACCAGAACAACCCGCT- 3′, (SEQ ID NO:11) 5′-TTCATGGACCAGAACAACCCGC- 3′, (SEQ ID NO:12) 5′-TTCATGGACCAGAACAACCCG- 3′, (SEQ ID NO:13) 5′-TTCATGGACCAGAACAACCC- 3′, (SEQ ID NO:14) 5′-TTCATGGACCAGAACAACC- 3′, (SEQ ID NO:15) 5′-ATTCATGGACCAGAACAACCCGC- 3′, (SEQ ID NO:16) 5′-AATTCATGGACCAGAACAACCCG- 3′, (SEQ ID NO:17) 5′-CAATTCATGGACCAGAACAACCC- 3′, (SEQ ID NO:18) 5′-CCAATTCATGGACCAGAACAACC- 3′, (SEQ ID NO:19) 5′-CAATTCATGGACCAGAACAAC- 3′, (SEQ ID NO:20) and 5′-AATTCATGGACCAGAACAACCCGCT- 3′, (SEQ ID NO:21) and P5 is selected from the group consisting of: 5′-CGACTGTCGGCGCTGGGGC-3′, (SEQ ID NO:22) 5′-CGACTGTCGGCGCTGGGGCC-3′, (SEQ ID NO:23) 5′-CGACTGTCGGCGCTGGGGCCC-3′, (SEQ ID NO:24) 5′-CGACTGTCGGCGCTGGGGCCCG-3′, (SEQ ID NO:25) 5′-CGACTGTCGGCGCTGGGGCCCGG-3′, (SEQ ID NO:26) 5′-CGACTGTCGGCGCTGGGGCCCGGC-3′, (SEQ ID NO:27) 5′-CCGACTGTCGGCGCTGGGGC-3′, (SEQ ID NO:28) 5′-GCCGACTGTCGGCGCTGGGGC-3′, (SEQ ID NO:29) 5′-CGCCGACTGTCGGCGCTGGGGC-3′, (SEQ ID NO:30) 5′-GCGCCGACTGTCGGCGCTGGGGC-3′, (SEQ ID NO:31) 5′-AGCGCCGACTGTCGGCGCTGGGGC-3′, (SEQ ID NO:32) 5′-GACTGTCGGCGCTGGGGCC-3′, (SEQ ID NO:33) 5′-ACTGTCGGCGCTGGGGCCC-3′, (SEQ ID NO:34) 5′-CTGTCGGCGCTGGGGCCCG-3′, (SEQ ID NO:35) 5′-CCGACTGTCGGCGCTGGGG-3′, (SEQ ID NO:36) 5′-GCCGACTGTCGGCGCTGGG-3′, (SEQ ID NO:37) and 5′-CGCCGACTGTCGGCGCTGGG-3′ (SEQ ID NO:38).