Patent ID: 7264965

Claim:
A method for attaching a complex to a target cell in vitro, wherein the complex comprises an HLA class I molecule or fragment thereof having a peptide binding groove, a recognition peptide, and attachment means comprising a linking polypeptide with high specific affinity for a target cell specific molecule on the surface of the target cell for selectively attaching said HLA class I molecule or fragment thereof to the target cell, wherein the said HLA class I molecule or fragment thereof is bound or attached to a recognition peptide in the peptide binding groove of said HLA class I molecule or fragment thereof, wherein the recognition peptide is arranged to be presented by said HLA class I molecule or fragment thereof for T cell recognition, and the HLA class I molecule or fragment thereof comprises a T cell binding portion, said method for attaching comprising the steps of: (i) introducing onto the surface of said target cell said attachment means; followed by, (ii) introducing onto the surface of said target cell said HLA class I molecule or fragment thereof having bound or attached thereto the recognition peptide.