Patent ID: 7148002

Claim:
A method of testing for an agent that modulates a guanine nucleotide exchange activity of a guanine nucleotide exchange factor (GEF) comprising: contacting (i) a mixture of (a) a polypeptide comprising a p115-Rho GEF, the p115-Rho GEF comprising an amino acid sequence encoded by a nucleotide sequence which hybridizes, or whose nucleic acid complement hybridizes, under stringent conditions to the nucleotide sequence set forth in SEQ ID NO:1, wherein said conditions comprise 5×SSPE, 10×Denhardts solution, 100 ug/ml salmon sperm DNA, 50% formamide, and 2% SDS, at 42° C., or a p115-Rho GEF fragment thereof, wherein said p115-Rho GEF or fragment has GEF activity, and (b) a polypeptide comprising a RhoA GTPase, or a fragment thereof to which the exchange factor can bind, with (ii) an agent; and detecting the presence or amount of guanine nucleotide exchange activity.