Patent ID: 8183011

Claim:
A method for making a polypeptide in a prokaryotic cell, said method comprising: (a) transfecting said prokaryotic cell with a nucleic acid sequence encoding said polypeptide, wherein the nucleic acid sequence encoding said polypeptide is mutated to replace mRNA interferase recognition sequences with an alternate triplet codon, wherein amino acid sequences of said polypeptide encoded by said mutated nucleic acid sequence are not altered by said mutating; (b) transfecting said prokaryotic cell with a nucleic acid sequence encoding an mRNA interferase, wherein said mRNA interferase recognizes said mRNA interferase recognition sequences; and (c) expressing the nucleic acid sequences of step (a) and (b) in said prokaryotic cell, wherein expressing the nucleic acid sequences of step (a) and (b) in said prokaryotic cell produces the polypeptide in said prokaryotic cell, wherein the mRNA recognition sequences are Adenine-Cytosine-Adenine (ACA) sequences and the mRNA interferase is MazF comprising SEQ ID NO: 2.