Patent ID: 7745131

Claim:
A method of identifying the erucic acid locus in a Brassica plant, wherein the erucic acid locus is a high erucic acid content locus or a low erucic acid content locus, comprising: admixing a primer consisting of SEQ ID NO: 25 and a primer selected from SEQ ID Nos. 26-45 with a DNA sample from a Brassica plant under conditions suitable for nucleic acid extension; and analyzing the extension product produced from a given DNA sample, wherein a single extension product having a first length indicates that the erucic acid locus is homozygous high erucic acid content, a single extension product having a second length indicates that the erucic acid locus is homozygous low erucic acid content and two extension products of both first and second lengths indicates that the erucic acid locus is heterozygous for high and low erucic acid content, and wherein the extension product having a first length is 2 bases longer than the extension product having a second length.