Patent ID: 8889416

Claim:
A method to analyze a biological sample, the method comprising positioning cellular material on top of a first support, applying a microisolation apparatus comprising a plurality of first access wells on top of the cellular material, said cellular material defining bottoms for the first access wells; filling the plurality of first access wells of the microisolation apparatus on top of the cellular material with a mixture comprising digestion agents and components necessary for a desired reaction; allowing the digestion agents to digest the cellular material thereby releasing cellular contents into the mixture in each well of the plurality of first access wells; inactivating the digestion agents; fixing the cellular contents in their respective wells; filling a plurality of second closed bottom access wells of a second support corresponding to the plurality of first access wells with an analytical reaction mixture; evaporating a fraction of the mixture to uniformly decrease the reaction mixture level across the plurality of second closed bottom access wells leaving a space at the top of the plurality of second closed bottom access wells; aligning the first support on top of the second support such that the plurality of first access wells face the plurality of second closed bottom access wells, securing the first and second support to form a secured support; and inverting the secured support to allow the analytical reaction mixture to flow from the second closed bottom access wells into the first access wells and via gravity contact the cellular contents in order to perform a reaction simultaneously but separately in each well of the secured support.