Patent ID: 7560226

Claim:
A method for distinguishing a leukemia of T cell, B cell, or myeloid lineage in a human subject comprising the steps of: providing a single assay device comprising a derivatised solid support selected from the group consisting of glass, cellulose, ceramic material, nitrocellulose, polyacrylamide, nylon, polystyrene, polystyrene derivatives, polyvinylidene difluoride, methacrylate, methacrylate derivatives, polyvinyl chloride, and polypropylene, the derivatised solid support having an array of immunoglobulin molecules immobilized in discrete regions on the derivatised solid support, wherein the immunoglobulin molecules are specific for the single cell surface marker antigens of CD3, CD4, CD8, CD14, CD19, and CD56, and wherein each immunoglobulin region specific for said single surface marker is present only once in the array; contacting a biological sample containing leukocytes with the assay device, wherein said biological sample is obtained from a human subject in need of a diagnosis of T cell, B cell, or myeloid lineage leukemia; allowing leukocytes in the biological sample to bind to the immunoglobulin molecules on the solid support via cell surface marker antigens on the leukocytes to form a pattern of binding on an array of discrete regions each being specific for a single cell surface marker presented only once in the array; and determining the relative scale of the pattern of simultaneous binding with which the cell surface marker antigens CD3, CD4, CD8, CD14, CD19, and CD56 on the leukocytes have bound to the immunoglobulin molecules on the array, wherein the relative scale of the pattern of CD3, CD4, CD8, CD14, CD19, and CD56 binding on the array distinguishes leukemia of T cell, B cell, or myeloid lineage in the subject.