Patent ID: 8153828

Claim:
A process for preparing a compound useful as a sensor for cytochrome P450 activity having the structure R 3 -L-Q said process comprising the step of reacting a compound of the formula H-Q in the presence of DMF/K 2 CO 3 or diisopropylethylamine/DMF at temperatures at about 0° C. to 5° C., with a compound of the formula R 3 -L-X wherein: X is a suitable leaving group selected from the group consisting of a halogen atom, a tosyl group, a mesyl group, and a triflate group; R 3 is an optionally substituted aryl; L has the chemical structure (−OCR 2 H) p —, wherein each R 2 is separately selected from the group consisting of a hydrogen atom, saturated C 1 -C 20 alkyl, unsaturated C 2 -C 20 alkenyl, unsaturated C 2 -C 20 alkynyl, substituted saturated C 1 -C 20 alkyl, substituted unsaturated C 2 -C 20 alkenyl, substituted unsaturated C 2 -C 20 alkynyl, C 3 -C 20 cycloalkyl, C 3 -C 20 cycloalkenyl, substituted saturated C 3 -C 20 cycloalkyl, substituted unsaturated C 3 -C 20 cycloalkenyl, aryl, substituted aryl, heteroaryl and substituted heteroaryl, and p is a positive integer selected from one to twelve; Q is attached to L through an ether linkage via the oxygen atom indicated by the following arrow, and has a structure selected from the group consisting of the following structures: wherein: R a , R b , R c , R d , and R e , are each separately selected from the group consisting of a hydrogen atom, a halogen atom, C 1 -C 20 alkyl, substituted C 1 -C 20 alkyl, perhalogenated alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, benzyl, heteroaryl, substituted heteroaryl, cyano, nitro, azido, —SR S , —OR O , —NR n1 R n2 , —N + R n1 R n2 R n3 , —P + R n1 R n2 R n3 , —COR C , —C(═NOR O )R C , —CSR C , —OCOR C , —OCONR n1 R n2 , —OCO 2 R C , —CONR n1 R n2 , —C(═N)NR n1 R n2 , —CO 2 R O , —SO 2 NR n1 R n2 , —SO 3 R O , —SO 2 R O , —PO(OR O ) 2 , —NR n1 CSNR n2 R n3 , —NR n1 C(═N)NR n2 R n3 , —NR n1 CONR n2 R n3 , —NR n1 COR C and —NR n1 S(═O) 2 R S ; R n1 , R n2 , R n3 , R O and R S are each separately selected from the group consisting of a hydrogen atom, C 1 -C 20 alkyl, substituted C 1 -C 20 alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, benzyl, heteroaryl, and substituted heteroaryl; R C is selected from the group consisting of a hydrogen atom, C 1 -C 20 alkyl, substituted C 1 -C 20 alkyl, perhalogenated alkyl, cycloalkyl, substituted cycloalkyl, aryl, substituted aryl, benzyl, heteroaryl, substituted heteroaryl, and cyano; and T is an oxygen atom.