Patent ID: 7704509

Claim:
A method for producing an infectious, self-replicating, recombinant human parainfluenza virus type 1 (HPIV 1) from one or more isolated polynucleotide molecules encoding said HPIV1, comprising: coexpressing in a cell or cell-free system one or more expression vector(s) comprising a polynucleotide molecule that encodes a partial or complete, recombinant HPIV 1 genome or antigenome and one or more polynucleotide molecules encoding PIV N, P and L proteins, thereby producing an infectious HPIV 1, said recombinant HPIV1 genome or antigenome including at least one mutation selected from the group consisting of: a mutation in the L protein Y942 to an amino acid selected from the group consisting of F, C, N, D, W, S, Q, G, T, V, M, L and A; a mutation in the L protein L992 to an amino acid selected from the group consisting of H, I, M, W, A, C, K, N, V and Y; a mutation in the L protein of E1711 to another amino acid; a mutation in the HN protein T553 to another amino acid; a mutation in the C protein R84 to another amino acid; a mutation in the C protein R84 to another amino acid and mutation of the P protein E119 to another amino acid; a mutation in the P protein E119 to another amino acid; a deletion of codons 10-15 of the C protein and codons 13-19 of the P protein; a deletion of codons 10-11 of the C protein and codons 13-15 of the P protein; a deletion of codons 12-13 of the C protein and codon 15-17 of the P protein; a deletion of codons 14-15 of the C protein and codons 17-19 of the P protein; a deletion of codons 168-170 of the C protein and codons 172-173 of the P protein.