Patent ID: 6936701

Claim:
A method for making a functionalized glycoconjugate, said method comprising the steps of: a) contacting a cell with a first monosaccharide comprising a first functional group; and b) incubating said cell under conditions whereby said cell (i) internalizes said first monosaccharide, (ii) biochemically processes said first monosaccharide into a second monosaccharide comprising a second functional group, (iii) conjugates said second monosaccharide to a carrier to form a glycoconjugate comprising a third functional group, and (iv) extracellularly expresses said glycoconjugate to form an extracellular glycoconjugate comprising a fourth, selectively reactive, functional group. wherein said first functional group is N-linked in said first monosaccharide, said first monosaccharide comprises a ManLev or FucLev, said cell is eukaryotic cell, and said fourth functional group is a covalently or non-covalently reactive group.