Patent ID: 6838083

Claim:
An antibiotic compound β-keto aliphatic acid ester isolated from Bacillus sp. IICT 001 and possessing the following spectral properties UV max (MeoH): 225 1 H NMR CDCI 3 (80 M H 2 ): 0.88 t (C H 3 ); 1.25 s, br (C H 2 )n; 2.16s (COC H 2 ); 3.68 s(COOCH 3 ) IR. V max(CHCl 3 ): cm −1 1730 (ester), 1670 (Carbonyl), wherein the antibiotic compound is made by a process comprising: preparing an at least 3 to 4 day old culture broth of Bacillus sp. with a medium comprising soya; extracting the resulting culture medium with a solvent at a temperature between 20° C. and 45° C. and a pH between 4.5 and 8.0; purifying the medium with a solvent by chromatography; and eluting with the same solvent to obtain the antimicrobial compound.