Patent ID: 6903077

Claim:
A non-viral nucleic acid delivery complex comprising a modular polypeptide comprising a nucleic acid binding domain that binds a nucleic acid molecule of more than 50 kilobases in length and is selected from the group consisting of Z1 (amino acids 1-231 of SEQ ID NO:2), Z2 (amino acids 225-349 of SEQ ID NO:2), Z2ΔP (amino acids 225-313 of SEQ ID NO:2), Z3 (amino acids 343-567 of SEQ ID NO:2), SEQ ID NO:4, SEQ ID NO:49, SEQ ID NO:51, and zinc finger domains that are coded by nucleic acid molecules that hybridize under stringent conditions to complements of nucleic acid molecules having a nucleotide sequence selected from the group consisting of nucleotides 1-702 of SEQ ID NO:1, nucleotides 682-1058 of SEQ ID NO:1, nucleotides 682-948 of SEQ ID NO:1, and nucleotides 1039-1704 of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:48, and SEQ ID NO:50; and a nucleic acid condensation domain that condenses a nucleic acid molecule of more than 50 kilobases in length and is a proline rich region of RIP60 selected from the group consisting of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:51, SEQ ID NO:68, amino acids 316-374 of SEQ ID NO:2, and domains that are coded by nucleic acid molecules that hybridize under stringent conditions to complements of nucleic acid molecules having a nucleotide sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:50, and nucleotides 682-948 of SEQ ID NO:1; and one or more polypeptides selected from the group consisting of a cell recognition domain, a protein transduction domain, a protein degradation domain, an intracellular targeting domain, a protein interaction domain, an epitope domain, and a protein purification domain, wherein the stringent conditions are hybridization at 65° C. in hybridization buffer (3.5×SSC, 0.02% Ficoll, 0.02% polyvinyl pyrolidone, 0.02% Bovine Serum Albumin, 2.5 mM NaH 2 PO 4 (pH7), 0.5% SDS, 2 mM EDTA); wherein SSC is 0.15M sodium chloride/0.015M sodium citrate, pH7; SDS is sodium dodecyl sulphate; and EDTA is ethylenediaminetetracetic acid.