Patent ID: 8435753

Claim:
A tissue differentiation method based on surface enhanced Raman scattering, which enables rapid tissue identification and enables determining, during surgery, an area where a cancerous tissue is located; the method comprising the steps of: preparing synthesized silver and/or gold nanoparticles, in which silver and/or gold colloidal solutions are used, by concentrating ( 101 ), cutting a few millimeters from a plurality of obtained tissue samples ( 102 ), placing the plurality of cut tissue samples in a porcelain container ( 103 ), adding 3-5 ml of liquid nitrogen or argon into the container and completely freezing the cut tissue samples ( 104 ), hereinafter referred to as “frozen tissue sample”, crushing the frozen tissue sample by pressing with a solid object and bringing the frozen tissue sample to a liquefied mixture ( 105 ), adding a part of the silver/and or gold nanoparticles directly into the liquefied tissue mixture ( 106 ), hereinafter referred to as “mixture sample”, transferring a small portion of the mixture sample onto a surface on which measurement will be performed ( 107 ), drying the mixture sample at room temperature ( 108 ) performing a measurement with a Raman device ( 109 ).