Patent ID: 8409623

Claim:
A method of manufacturing a cancellous bone graft substitute, comprising: (a) cutting an extracted allogeneic or xenogeneic bone tissue into block patterns and removing soft tissue; (b) defatting, dehydrating, and disinfecting the allogeneic or xenogeneic bone tissue of step (a) by adding an aqueous ethanol solution to the bone tissue and applying supersonic cavitation; (c) removing residual soft tissue from the allogeneic or xenogeneic bone tissue which has been defatted, dehydrated, and disinfected; (d) sterilizing and oxidizing the allogeneic or xenogeneic bone tissue of step (c) by adding peroxide to bone tissue and applying supersonic cavitation; (e) cutting the allogeneic or xenogeneic bone tissue, which has been disinfected and oxidized, into bone particles having hexahedral shapes; (f) demineralizing the bone particles of step (e) by adding hydrochloric acid to the bone particles and applying supersonic cavitation; (g) cleaning the demineralized bone particles of step (f) by adding an aqueous ethanol solution and applying supersonic cavitation; (h) neutralizing the cleaned bone particles using a phosphate buffered saline (PBS); (i) steeping the neutralized bone particles of step (g) by adding a Radio Protective Stable Chemical (RSC) solution and applying supersonic cavitation, said RSC solution maintains osteoconductive and osteoinductive capabilities of said bone particle, protects osteogenesis induction factors, and prevents the bone particles from being rapidly dissolved or inactivated after being implanted in a living body; (j) cleaning the bone particles which have steeped in the RSC solution, using purified water and then drying the bone particles by removing the purified water; and (k) vacuum-freeze-drying the dried bone particles, thereby manufacturing a cancellous bone graft substitute.