Patent ID: 8715924

Claim:
A process for determining a concentration of nucleic acids in a sample in a microfluidic device with a first chamber and a second chamber, comprising the following steps: a) introducing the sample into the first chamber via a first supply channel connected to the first chamber and providing a first supply of reagents for an amplification reaction, which is to be carried out in cycles, into said first chamber via a second supply channel connected to the first chamber; b) carrying out a number of said cycles of said amplification reaction such that the nucleic acids are amplified; c) transferring a defined volume from the first chamber, which is a fraction of a total sample volume in the first chamber and which has said amplified nucleic acids, into said second chamber via a channel connecting the first chamber to the second chamber and simultaneously replacing the transferred defined volume by providing a fresh, second supply of the reagents for said amplification reaction in the first chamber by supplying the fresh, second supply of the reagents through the second supply channel using a volume transfer device located outside of the supply channel, wherein a remaining fraction of the total sample volume remains in the first chamber for subsequent amplification; d) determining said concentration of the amplified nucleic acids in the second chamber equipped with a device for determining said concentration; and e) repeating steps b)-d) until said determined concentration of the amplified nucleic acids in said second chamber is within a set range, wherein said set range is a measurement range of said device for determining said concentrations.