Patent ID: 8467973

Claim:
A method of preparing alloreactive cytotoxic T cells comprising: providing stimulator information, wherein the stimulator information comprises patient cell antigen information determined through one or more of serotyping or a high or intermediate resolution molecular sequencing method of major histocompatibility complex (MHC) information; providing responder information, wherein the responder information comprises donor cell antigen information determined through one or more of serotyping or a high or intermediate resolution molecular sequencing method of MHC information; comparing the stimulator information to the responder information; identifying presence or absence of a partial mismatch between the stimulator information and the responder information by analyzing and comparing HLA alleles of donor and patient, the partial mismatch comprising 1-6 antigen units or 1-10 amino acid differences in corresponding HLA molecules of the donor and the patient; selecting a patient cell and donor cell pair to prepare alloreactive cytotoxic T cells based on the presence of the partial mismatch; and combining cells of the patient with cells of the donor to generate the alloreactive cytotoxic T cells in an alloreactive cytotoxic T cell reaction based on the patient cell and donor cell pair, whereby the alloreactive cytotoxic T cells are produced, the alloreactive cytotoxic T cells being activated against the cells of the patient which present the antigen.