Patent ID: 7176001

Claim:
A method for the manufacture and purification of cyclosporin A (CsA) comprising: loading a contained bioreactor with solid state nutritive matrix and sterilizing it, wherein the bioreactor comprises multiple stacked modules, each with a base connected to a frame, and wherein each base contains a plurality of communicating and noncommunicating channels, mixing the said sterilized solid state nutritive matrix with Fusarium sp. used as inoculum, incubating the said inoculated solid state nutritive matrix for 4–6 days at 25–30° C., extracting the fermented matrix with an organic solvent, concentrating the organic solvent extract and treating with 0.1 to 5.0% alum for 2 to 6 hours to obtain a CsA precipitate, followed by filtration using a filter aid, dissolving the CsA precipitate in a hydrocarbon organic solvent and concentrating to get a residue, treating the residue with alcohol and subjecting the residue to gel filtration to produce CsA containing fractions, pooling the CsA containing fractions and subjecting to silica gel chromatography, elution and removing the organic solvent by distillation to produce a concentrate, dissolving the concentrate obtained in 2 to 6 times the volume of an organic solvent and allowing to chill at −10 to −45° C. to 2 to 8 hours, and filtering the organic solvent to get pure cyclosporin A.