Patent ID: 7803619

Claim:
A method for making mouse embryoid bodies having a uniform size, comprising (a) genetically modifying a mouse embryonic stem (ES) cell to contain a transgene comprising a reporter gene and selectable marker, both operably linked to a developmentally regulated promoter and operably separated by an internal ribosome entry site (IRES), (b) growing the ES cells in the presence of feeder cells and leukemia inhibitory factor (LIF), (c) removing the feeder cells, (d) dissociating the ES cells in differentiation media, (e) placing approximately 1,000 dissociated ES cells of step (d) in a 200 μl volume into each well of a plurality of wells of a polyvinyl carbonate multiwell plate in media, wherein the multiwell plate is not subjected to centrifugation and a single mouse embryoid body (EB), that has a uniform diameter relative to other EBs formed in, is produced per well in at least 92% of the wells.