Patent ID: 7097975

Claim:
A primer set comprising oligonucleotide primers consisting of an eighteen to twenty-four nucleotide contiguous portion of SEQ ID NO:2 or of its complement, wherein said primer set comprises the contiguous sequence 5′-TAAGATCTTTGGGCAATGGT-3′ (SEQ ID NO:3) and the sequence 5′-GCAACACTCAAAATCCAACAAT-3′ (SEQ ID NO:4) or at least an eighteen nucleotide contiguous portion of SEQ ID NO: 3 and SEQ ID NO:4, and wherein said primer set encompasses at least a single nucleotide difference between P. pachyrhizi and P. meibomiae at the 3′ terminus of the primer when the ITS1 or ITS2 regions are aligned and when a comparison as depicted in FIG. 3 is made between said ITS1 or ITS2 regions, and wherein said primer set specifically hybridizes to a region of SEQ ID NO:2 or its complement, and wherein said primer set distinguishes P. pachyrhizi from P. meibomiae.