Patent ID: 8883485

Claim:
A method for decolorizing a dye, comprising contacting the dye with a composition comprising: a perhydrolase enzyme having at least 90% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO: 1 or SEQ ID NO: 3, an ester substrate for the perhydrolase enzyme, having carboxylic acid ester moieties, and a hydrogen peroxide source; wherein the carboxylic acid ester moieties of the ester substrate are provided at: (i) about 20-20,000 times the molar amount of dye to be decolorized, and (ii) a molar ratio of at least 2×10 5 /1 with respect to the perhydrolase enzyme, and; wherein a peracid is produced by catalytic action of the perhydrolase enzyme on the ester substrate in the presence of hydrogen peroxide; wherein the dye is contacted with the composition for a length of time and under conditions suitable to produce an amount of peracid sufficient to decolorize at least a portion of the dye, and wherein the dye is present in an effluent.