Patent ID: 7662923

Claim:
A method for increasing clot formation in a mammal, comprising administering an amount of a mutant Factor VII or VIIa polypeptide that is effective to increase clot formation in said mammal, wherein said mutant Factor VII or VIIa polypeptide comprises a modified gamma-carboxyglutamic acid (GLA) domain that enhances membrane binding affinity of said polypeptide relative to a corresponding native Factor VII or Factor VIIa polypeptide, and wherein said modified GLA domain has 2 to 5 amino acid substitutions and comprises a glutamine residue substituted at amino acid position 10 and an amino acid substitution at one or more amino acid positions selected from positions 28, 32, 33, and 34 of SEQ ID NO: 3 or SEQ ID NO: 4.