Patent ID: 7781229

Claim:
A horseradish peroxidase chemiluminescent assay formulation comprising: at least one organic chemiluminescent acridane formulation corresponding to the formula: where X is oxygen, nitrogen or sulfur; n is an integer from 1 to 2, and when n is 1, R is selected from the group consisting of a deuterium atom or a deuterium atom-containing group, a water solubility enhancing group, an enhancer, a 1,2-dioxetane, a linear or branched C 1 to C 6 alkyl, substituted C 1 to C 6 alkyl, aryl, alkylaryl, arylalkyl, alkyl alcohol, alkyl nitrile, when n=2, R is methyl, deuterated methyl, phenyl, deuterated phenyl or substituted; phenyl; R 1 is a linear or branched C 1 to C 6 alkyl or deuterated alkyl, aryl or deuterated aryl, arylalkyl, alkylaryl, heteroalkyl, alkylalkene, arylalkene, alkylnitrile, alkylalcohol and alkylacid; R 2 and R 3 may be alkyl or deuterated alkyl, methoxy or deuterated methoxy, Cl, Br or CN and wherein at least one of R, R 1 , R 2 and R 3 contain a deuterium atom or deuterium atom-containing group.