Patent ID: 8586291

Claim:
A method of determining whether a test compound inhibits the tyrosine kinase activity of a mutant Bcr-Abl polypeptide, wherein the mutant Bcr-Abl polypeptide comprises a M244V, Y253H, E255K, T277A, T277P, M278K, V299L, M278L, V304D, V304A, T315I, F317L, E355G, F359C, F359I, F359V, L364I, L364H, V379I, H396R or H396K amino acid substitution in the Bcr-Abl polypeptide sequence shown in SEQ ID NO: 1, the method comprising the steps of: contacting a mammalian cell that expresses said mutant Bcr-Abl polypeptide with the test compound; and monitoring the mammalian cell for the tyrosine kinase activity of the mutant Bcr-Abl polypeptide, wherein an inhibition in tyrosine kinase activity in the presence of the test compound as compared to the absence of the test compound identifies the test compound as an inhibitor of the mutant Bcr-Abl polypeptide.