Patent ID: 7498146

Claim:
A method of preparing a stable Factor VIII/von Willebrand Factor (vWF)-complex comprising: a) subjecting an impure protein solution of Factor VIII or a Factor VIII/vWF-complex to a chromatographic treatment to obtain a purified Factor VIII, a purified high-molecular fraction of vWF molecules, or a purified Factor VIII/vWF-complex; and b) admixing the purified high-molecular fraction of vWF molecules to the purified Factor VIII or the purified Factor VIII/vWF-complex, thereby preparing a stable Factor VIII/vWF-complex, wherein the stable Factor VII/vWF-complex has a molar ratio of Factor VIII to vWF of between 0.01 and 100, and wherein the high-molecular fraction of vWF molecules has a specific platelet agglutination activity of at least 50 U/mg vWF:Ag.