Patent ID: 7727738

Claim:
A method to generate analogs of coenzyme A, comprising: phosphorylating a pantetheine analog or a derivative thereof to form a phosphopantetheine analog or a derivative thereof, the pantetheine analog or derivative thereof comprising a reporter or a bioorthogonal tag having reactivity with a reporter, adenylating the phosphopantetheine analog or a derivative thereof to form a dephosphocoenzyme A analog or a derivative thereof, and, phosphorylating the 3′-hydroxyl of the dephosphocoenzyme A analog or a derivative thereof to form a coenzyme A analog or a derivative thereof, wherein the phosphorylating of the pantetheine analog or a derivative thereof, the adenylating, or the phosphorylating the 3′-hydroxyl of the dephosphocoenzyme A analog or a derivative thereof is catalyzed by one or more native enzymes.