Patent ID: 8354245

Claim:
A method of assaying glucose-6-phosphate dehydrogenase (G6PD) in a liquid sample, the method comprising (a) applying the sample to a chromatographic strip, the chromatographic strip comprising at least a first pad, a second pad, and an intermediate pad positioned on an adhesive backing, wherein said first pad is a sample receiving pad, which has been treated to concentrate the sample as it flows therethrough by removing a portion of its liquid content, the second pad comprises a mixture of dry components, including a substrate for G6PD, and the intermediate pad is interposed between the sample receiving pad and the substrate pad, wherein the intermediate pad has been treated with an immovable deposit of at least one substance that removes and binds at least one sample component that would otherwise interfere with or obscure the result of the assay for G6PD activity; and (b) allowing said sample to flow through the first pad and the intermediate pad, and into the second pad, thereby mobilizing the mixture of dry components, whereby the dry components are reconstituted by contact with the liquid sample and a color indicative of the presence or concentration of G6PD is formed at a forward flow front of the chromatographic strip.