Patent ID: 7192761

Claim:
A plasmid comprising: (a) at least one marker gene comprising a Tn3 Amp R gene, or an ampicillin, tetracycline or chloramphenicol resistance gene operably linked to a first promoter comprising one or more pairs of −35 and −10 promoter elements of an Actinobacillus succinogenes pckA gene; (b) an origin of replication comprising at least a 1.5 kB PstI-HaeII fragment of a pGZRS-1 replicon so as to be functional in Actinobacillus succinogenes; c) a second promoter comprising one or more pairs of −35 and −10 elements of the Actinobacillus succinogenes pckA gene; and (d) a cloning site downstream from the second promoter.