Patent ID: 7695917

Claim:
An in vitro method of rescuing a mutant gonadotropin-releasing hormone receptor (GnRHR), comprising contacting a cell expressing the mutant GnRHR with [(2S)-2-[5-[2-(2-azabicyclo[2.2.2]oct-2-yl)-1,1-dimethyl-2-oxoethyl]-2-(3,5-dimethylphenyl)-1H-indol-3-yl]-N-(2-pyridin-4-ylethyl)propan-1-amine] (IN3), and rescue of the mutant GnRHR results in an at least 25% increased binding of the mutant receptor to buserelin, an at least 25% increased GnRH agonists-stimulated inositol) phosphate (IP) production, or an at least 25% increase in surface-bound receptor-ligand complex internalized, when compared to an absence of IN3.