Patent ID: 7018801

Claim:
A method for identifying a peptide-peptide interaction comprising: (a) providing a first fusion construct comprising target peptide of 8 to 15 residues fused to a first DNA binding domain (DBD); (b) providing a second fusion construct comprising a library encoded peptide (LEP) of 5 to 50 residues fused to second DBD, wherein said second DBD works as a complex with said first DBD to facilitate binding of said complex to a prokaryotic operator region; (c) contacting said first and second fusion constructs in a prokaryotic host cell which comprises said prokaryotic operator region, wherein said prokaryotic operator region is operationally linked to a coding region for one or more indicator polypeptides; and (d) determining binding of said complex to said operator region, whereby binding of said complex to said operator region identifies said LEP as a binding partner for said target peptide.