Patent ID: 7459696

Claim:
A method of calibrating spectral data obtained from a tissue sample, the method comprising the steps of: (a) illuminating a first calibration target and obtaining a first set of calibration data from a plurality of spaced-apart locations on said first calibration target; (b) illuminating a second calibration target and obtaining a second set of calibration data from a plurality of spaced-apart locations on said second calibration target; (c) illuminating a tissue sample and obtaining a set of spectral data from spaced-apart locations of said tissue sample, wherein at least some of said spaced-apart locations of said tissue sample correspond to said spaced-apart locations on said first calibration target and said spaced-apart locations on said second calibration target, and wherein said tissue sample comprises in vivo cervical, vaginal, or colorectal tissue; and (d) calibrating said spectral data obtained from said tissue sample using said first set of calibration data and said second set of calibration data, thereby to produce calibrated data, wherein said first calibration target has a reflectance of about 60% and said second calibration target has a reflectance of about 10%.