Patent ID: 8017015

Claim:
A chromatographic method for detecting one or more analytes in a body fluid sample containing a mixture of other substances, said method comprising: a) separating said one or more analytes from said other, substances in a mixture using a first chromatography column comprising a monolithic sorbent having macropores and mesopores, using a first mobile phase under conditions such that said one or more analytes are retained on said first column and other substances are removed; wherein said one or more analytes retained on said first column have a molecular weight of less than about 5,000 daltons, b) eluting said one or more retained analytes by applying a second mobile phase to the column, c) directing the eluted one or more analytes to a second chromatography column in-line with said first column for further separation, and d) detecting said one or more analytes following the separation in step c).