Patent ID: 7893221

Claim:
A method of forming a dimeric, biologically active transforming growth factor beta 3 comprising adding solubilized, unfolded monomeric transforming growth factor beta 3 to a solution comprising: (i) 0.7 M 2-(cyclohexylamino)-ethanesulfonic acid (CHES); (ii) a low molecular weight sulfhydryl/disulfide redox system comprising about 2 mM reduced glutathione and about 0.4 mM oxidized glutathione; and (iii) 1 M NaCl, wherein the concentration of transforming growth factor beta 3 in the solution is about 0.25mg/mL and the solution is at a pH of about 9.5, and incubating the unfolded monomeric transforming growth factor beta 3 in the solution until a dimeric biologically active transforming growth factor beta 3 is formed, wherein the forming of dimeric biologically active transforming growth factor beta 3 in the solution is completed within about 2 days.