Patent ID: 7122382

Claim:
A method for determining interacting proteins, comprising: i) synthesizing in a cell-free synthesis method at least one first group of proteins wherein said first group comprises one or more proteins bearing a label for detection and at least one second group of proteins wherein said second group comprises one or more proteins bearing a modification that provides for solid-phase separation; ii) mixing said at least one first group of proteins bearing a label for detection with said at least one second group of proteins bearing a modification that provides for solid-phase separation; iii) separating, by binding to a solid phase, one or more protein pairs formed by the interaction between a protein of the first group of proteins and a protein of the second group of proteins from proteins having labels for detection that have not formed protein pairs; iv) detecting the protein pair by detecting the label for detection; v) identifying at least one first group of proteins and at least one second group of proteins that include at least one protein pair; vi) dividing the at least one first group of proteins including a protein pair into at least two sub-groups and dividing the at least one second group of proteins including a protein pair into at least two sub-groups; and vii) repeating steps i) to vi) for each sub-group until the proteins forming protein pairs are determined.