Patent ID: 7060500

Claim:
A method of obtaining an animal cell that contains a predetermined genetic change in a target gene which comprises: (a) providing a population of animal cells in a culture media; (b) adding to the culture media a composition comprising: (i) a single-stranded oligodeoxynucleotide having a 3′ end nucleotide, a 5′ end nucleotide, having at least 31 deoxynucleotides and not more than 59 deoxynucleotides, and having a sequence comprising at least two regions each of at least 8 deoxynucleotides that are each, respectively, identical to two regions of the targeted chromosomal gene, which regions together are at least 24 deoxynucleotides in length, and which regions are separated by at least one deoxynucleotide in the sequence of the targeted chromosomal gene or in the sequence of the oligodeoxynucleotide or both, such that the sequence of the oligodeoxynucleotide is not identical to the sequence of the targeted chromosomal gene; and (ii) a macromolecular carrier selected from the group consisting of: (A) an aqueous-cored lipid vesicle, wherein the aqueous core contains the single-stranded oligodeoxynucleotide, (B) a lipid nanosphere, which comprises a lipophilic salt of the single-stranded oligodeoxynucleotide, and (C) a polycation having an average molecular weight of between 500 daltons and 1.3 Md wherein the polycation forms a salt with the single-stranded oligodeoxynucleotide; and (c) identifying a cell of the population having the predetermined genetic change.