Patent ID: 7655790

Claim:
A process comprising the steps of: a) synthesizing a nucleic acid molecule comprising one or more ribonucleotides, using a method selected from the group consisting of solid phase phosphoramidite, solution phase phosphoramidite, solid phase H-phosphonate, solution phase H-phosphonate, hybrid phase phosphoramidite, and hybrid phase H-phosphonate-based synthetic methods; b) contacting said nucleic acid molecule from step (a) with aqueous alkylamine, trialkylamine, or alkylamine and trialkylamine, under conditions suitable for the removal of any 2′-amino protecting groups, exocyclic amino (base) protecting groups and/or phosphate protecting groups, which may be individually present or absent, from said molecule; c) contacting reaction mixture having said nucleic acid molecule from step (b) with a polar solvent and trialkylamine.hydrogen fluoride under conditions suitable for the removal of a 2′-OH protecting group; d) loading reaction mixture having said nucleic acid molecule from step (c) onto a media selected from the group consisting of Pharmacia Source Q15, Biorad Macroprep 25Q, Pharmacia Q-sepharose, Perceptive POROS HQ, TOSOHAAS Q-5PW-HR, Q-5PW, and super Q-5PW media in a suitable buffer comprising buffers selected from the group consisting of water, 20% ethanol in about 20 mM sodium phosphate and about 0.1 M NaCl and acetonitrile in about 20 mM sodium phosphate and about 0.1 M NaCl; e) applying a purification gradient using a suitable elution buffer, analyzing the fractions and allowing for the pure fractions to be pooled and desalted.