Patent ID: 8492122

Claim:
A method for assembling a modulatable fusion polypeptide, comprising: a) creating an insertion nucleic acid sequence library comprising the steps of: i) obtaining an insertion nucleic acid sequence which encodes a polypeptide that recognizes a signal of interest; ii) ligating the insertion nucleic acid sequence under conditions sufficient to circularize the insertion nucleic acid sequence; iii) digesting the insertion nucleic acid sequence of ii) under conditions sufficient to randomly introduce a double-stranded break to create the insertion nucleic acid sequence library; b) creating an acceptor nucleic acid sequence library comprising the steps of: i) obtaining an acceptor nucleic acid sequence which encodes a polypeptide that produces a measurable change in a desired state, provided the measurable change in state is not fluorescence; ii) ligating the acceptor nucleic acid sequence under conditions sufficient to circularize an insertion nucleic acid sequence; iii) digesting the acceptor nucleic acid sequence of ii) under conditions sufficient to randomly introduce a double-stranded break to create an acceptor nucleic acid sequence library; c) ligating the nucleic acids of the libraries of a) and b) under sufficient conditions such that an insertion nucleic acid sequence will randomly insert into the randomly introduced double strand break in an acceptor nucleic acid sequence; d) transforming a suitable host with the ligated libraries of c); e) selecting for a transformed host expressing the modulatable fusion polypeptide by exposing the transformed host to the signal of interest and identifying the transformed host which exhibits a measurable change of the desired state.