Patent ID: 8168426

Claim:
A multiple inducible gene modulation system consisting of two individually operable heterologous gene modulation systems, wherein each individually operable heterologous gene modulation system comprises: a) a first gene expression cassette comprising a polynucleotide sequence that encodes a polypeptide comprising: A) a DNA binding domain that recognizes a response element associated with a gene of interest; B) an ecdysone receptor ligand binding domain; and b) a second gene expression cassette comprising a polynucleotide sequence comprising a promoter operably linked to a polynucleotide sequence that encodes a second polypeptide comprising a nuclear receptor ligand binding domain and a transactivation domain; and wherein each individually operable heterologous gene modulation system is orthogonal to the other individually operable heterologous gene modulation system present in the multiple inducible gene modulation system.