Patent ID: 7901905

Claim:
A method of constructing a synthetic polynucleotide from which a polypeptide is producible at a higher level in a Chinese Hamster Ovary (CHO) cell than from a parent polynucleotide encoding the same polypeptide, the method comprising: selecting a first codon of the parent polynucleotide for replacement with a synonymous codon, wherein the synonymous codon is selected on the basis that it exhibits a higher translational efficiency in the CHO cell than the first codon in a comparison of translational efficiencies of codons in test CHO cells; and replacing the first codon with the synonymous codon to construct the synthetic polynucleotide, wherein the first and synonymous codons are selected from the following Table: Synonymous First Codon Codon Ala GCC Ala GCA Ala GCC Ala GCG Ala GCC Ala GCT Ala GCT Ala GCA Ala GCG Ala GCA Arg CGC Arg AGA Arg CGC Arg CGA Arg CGC Arg CGG Arg CGC Arg CGT Arg CGC Arg AGG Asp GAC Asp GAT Glu GAG Glu GAA Gly GGT Gly GGA Gly GGC Gly GGA Gly GGC Gly GGG Gly GGG Gly GGA Leu CTT Leu TTG Leu CTG Leu CTC Leu CTG Leu TTG Leu CTA Leu TTG Leu CTT Leu CTA Leu TTA Leu CTA Phe TTC Phe TTT Thr ACC Thr ACT Thr ACC Thr ACG Thr ACC Thr ACA .