Patent ID: 7632510

Claim:
A method of eliciting an immune response in a subject against infection by a flavivirus , comprising administering to the subject an effective amount of a composition comprising: an isolated nucleic acid comprising a transcriptional unit encoding an engineered Japanese Encephalitis Virus (JEV) signal sequence, which engineered JEV signal sequence comprises SEQ ID NO:14, and an immunogenic flavivirus antigen of a flavivirus other than JEV, wherein the immunogenic flavivirus antigen of a flavivirus other than JEV is selected from the group consisting of an M protein of a flavivirus , an E protein of a flavivirus , both an M protein and an E protein of a flavivirus , a portion of an M protein of a flavivirus , a portion of an E protein of a flavivirus and both a portion of an M protein of a flavivirus and a portion of an E protein of a flavivirus , or any combination thereof, and wherein the transcriptional unit directs the synthesis of the immunogenic flavivirus antigen; and a pharmaceutically acceptable carrier.