Patent ID: RE41461

Claim:
A method for cleaving hexosamine-glucuronic acid linkages in linear polysacharides polysaccharides of D-glucosamine linked to hexuronic acid comprising reacting heparin or heparan sulfate with a purified heparinase selected from the group consisting of heparinase II present in Flavobacterium heparinum heparinase II free of lyase activity other than heparinase II activity, having a molecular weight of 84,100, cleaving heparin and heparan sulfate and having a pH optimum pI of 8.9-9.1 and heparinase III which is expressed in Flavobacterium heparinum heparinase III free of lyase activity other than heparinase III activity, having a molecular weight of 70,800, cleaving heparan sulfate, and having a pH optimum pI of 9.9-10.1.