Patent ID: 7312090

Claim:
A method of detecting binding of molecules released from cells grown in a semi-permeable internal sleeve held in contact with or in close proximity to a colorimetric resonant reflectance optical biosensor to one or more specific binding substances, wherein the one or more specific binding substances are immobilized on the surface of the biosensor, the method comprising: (a) detecting a colorimetric resonant reflectance optical PWV of a distinct location of the biosensor; (b) growing cells in the semi-permeable internal sleeve held in contact with or in close proximity to the colorimetric resonant reflectance optical biosensor at the distinct location; (c) detecting the colorimetric resonant reflectance optical PWV of the distinct location; (d) comparing the PWV of step (a) to the PWV of step (c); wherein binding of the molecules released from cells grown in the semi-permeable internal sleeve held in contact with or in close proximity to the colorimetric resonant reflectance optical biosensor to the one or more specific binding substances is detected.