Patent ID: 7118907

Claim:
A microfluidic device useful for analyzing a reaction, the device comprising: a substrate; at least two microchannels disposed on the substrate, wherein said microchannels are in fluid communication with each other; an immobilization zone within a first one of said microchannels, wherein at least a portion of a reactant is immobilized proximal the immobilization zone to allow for a reaction to occur so as to produce a reaction product; and a detection zone within a second one of said microchannels, wherein there is substantially no electric field within the first microchannel proximal the immobilization zone, and wherein a biasing force moves the reaction product into the detection zone within the second microchannel, wherein the reactant includes a charge-switch nucleotide probe, and wherein the probe is a nucleotide triphosphate and the terminal phosphate includes a fluorophore attached thereto.