Patent ID: 7094593

Claim:
A yeast cell comprising a nucleic acid sequence encoding a mutated, heterologous G protein-coupled receptor, wherein the mutation is a deletion mutation in (1) the 3 rd intracellular loop resulting in a 44 amino acid third intracellular loop comprising 22 residues proximal to the 5 th and the 6 th transmembrane domains or (2) the C-terminus, said mutation improving the function of said heterologous G protein-coupled receptor by causing it to couple more efficiently with a heterotrimeric G protein compared to a wild-type form of the heterologous G protein-coupled receptor, and wherein the mutated G protein-coupled receptor is selected from the group consisting of a muscarinic acetylcholine receptor, a cholecystokinin CCKB receptor, a somatostatin receptor, and a serotonin receptor.