Patent ID: 6838549

Claim:
A preparation of isolated and purified human pluripotent colony stimulating factor for stimulation of proliferation and differentiation of pluripotent progenitor cells to all major blood cell types, wherein the major blood cell types are selected from the group consisting of granulocytes, macrophages, eosinophils and megakaryocytes; and wherein the human pluripotent colony stimulating factor has the following characteristics: a) a molecular weight of about 19,600 daltons under reducing conditions and non-reducing conditions as determined by SDS-PAGE; b) a molecular weight of about 32,000 daltons as determined by gel filtration; c) an isoelectric point of about 5.5; d) the ability to induce differentiation of the leukemia cell line WEHI-3B (D+); and e) the ability to stimulate growth of early hematopoietic progenitor cells.