Patent ID: 7611889

Claim:
A method of producing a microarray, comprising: providing a nonporous solid substrate having an amino functional group attached thereto; dissolving a nucleic acid functionalized with an amino group in a solution comprising polyethylene glycol (PEG) to form a solution containing the nucleic acid and the PEGS; and transferring the solution containing the nucleic acid and the PEG to the substrate, in order to noncovalently immobilize the nucleic acid on the substrate via noncovalent intervention of the PEG; wherein the nonporous solid substrate having an amino functional group attached thereto is a solid substrate with a surface coated with γ-aminopropyltriethoxysilane (GAPS), and the nucleic acid functionalized with an amino group is DNA functionalized with an aminohexyl group at its 5′ end, and wherein a concentration of the PEG is in a range of 0.4 to 8.0 mM.