Patent ID: 7232578

Claim:
A method for the preparation of an anti-cancer cell differentiation agent pharmaceutical composition, comprising the steps of: (1) collecting human urine, in which the concentration of creatinine is in the range of 1.2-3.7 g/l, and acidifying the urine; (2) filtering the collected urine to remove substances with a molecular weight greater than about 10,000 Daltons; (3) passing the collected and filtered urine through an adsorbent to yield an adsorbed urine derivative; (4) extracting the adsorbed urine derivative with an organic solvent; and (5) diluting the extracted adsorbed urine derivative with water, and removing pyrogens by ultrafiltration through a filter, to yield thereby the anti-cancer cell differentiation agent pharmaceutical composition comprising a predetermined amount of differentiation inducers, wherein the differentiation inducers are PP-0 and OA-0.79, wherein PP-0 is a peptide conjugated with a pigment material which emerges from a gel filtration column of Ultrogel AcA202 with a K av value of 0, and OA-79 is an organic acid which emerges from the same gel column with a K av value of 0.79.