Patent ID: 7105158

Claim:
A method of administering a substance to a subject having an immune modulated disease, comprising the steps of: (a) preparing a microencapsulated composition, comprising the steps of i) providing an aqueous phase of a solubilized amount of an antibody or protein material to be encapsulated, said material being solubilized in phosphate buffered saline; ii) mixing said solubilized material with an amount of biodegradable and nonantigenic albumin dissolved in water or phosphate buffered saline to form an aqueous phase mixture; iii) adding said aqueous phase mixture of step ii) to an amount of olive oil; iv) emulsifying said aqueous phase mixture formed in step iii) to form microspheres of encapsulated material; and, v) contacting said encapsulated microspheres with a crosslinking agent for a time sufficient to crosslink at least a portion of said microspheres, such that said crosslinked microspheres have bioactivity and such that denaturization is substantially prevented; and, b) administering to said subject a therapeutically effective amount of said crosslinked microspheres.