Patent ID: 8507201

Claim:
An article of manufacture, comprising: a pair of IS481 primers, wherein said pair of IS481 primers comprise a first IS481 primer and a second IS481 primer, wherein said first IS481 primer comprises the sequence 5′-CCA GTT CCT CAA GGA CGC-3′ (SEQ ID NO:1), and wherein said second IS481 primer comprises the sequence 5′-GAG TTC TGG TAG GTG TGA GCG TA-3′ (SEQ ID NO:2); a pair of IS481 probes, wherein said pair of IS481 probes comprises a first IS481 probe and a second IS481 probe, wherein said first IS481 probe comprises the sequence 5′-CAC CGC TTT ACC CGA CCT TAC CGC CCA C-3′ (SEQ ID NO:3), and wherein said second IS481 probe comprises the sequence 5′-GAC CAA TGG CAA GGC TCG AAC GCT TCA TC-3′ (SEQ ID NO:11); and a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.