Patent ID: 7943818

Claim:
A method of producing a soybean plant comprising a linolenic acid content of less than about 6% of total seed fatty acids by weight and an oleic acid content of about 55% to about 80% of total seed fatty acids by weight, comprising the steps of: a) making one or more soybean plants that comprise a transgene that decreases the expression of an endogenous soybean FAD2-1 gene and at least one loss-of-function mutation in an endogenous soybean FAD3 gene; b) obtaining at least one seed from said soybean plant obtained in step (a); c) determining a percentage of the total seed fatty acid content by weight of linolenic acid and oleic acid for said seed of step (b); and, d) identifying a soybean plant that yields seed having a seed fatty acid composition comprising a linolenic acid content of less than about 6% of total seed fatty acids by weight and an oleic acid content of about 55% to about 80% of total seed fatty acids by weight.