Patent ID: 7241588

Claim:
A method for producing a novel β-lactam antibiotic selected from the group consisting of: from a protoplast fusion strain CCRC930060, comprising the steps of: (a) culturing the protoplast fusion strain CCRC930060 which is obtained from the protoplast fusion of Penicillium chrysogeum (ATCC 48271) and Cephalosporium acremonium (ATCC 48272) with the following medium for fermentative culture: Component Amount (weight %) Sucrose 12 Lard 0.1 Ammonium sulfate 2 Di-potassium hydrogen phosphate 0.05 Sodium citrate 0.4 Phenoxyacetic acid 1.12 to obtain a liquid culture suspension; (b) processing said liquid culture suspension of step (a) to obtain a ferment filtrate which is then lyophilized to provide a powder, added with a volume of acetone, stirred at room temperature for extraction, and filtered; (c) collecting the filtrate obtained in step (b) and concentrating the filtrate by decompression; (d) filtering the concentrate of step (c) by a filter membrane of 0.22 μm to provide a product; (e) analyzing the product of step (d) by preparation type HPLC using a mobile phase to obtain an eluent having said antibiotic; (f) isolating said antibiotic in the eluent obtained in step (e) by a bacteriostatic test and a pitting test; and (g) concentrating the isolated eluent of step (f) to provide said antibiotic.