Patent ID: 7033760

Claim:
A method of detecting at least one hybridization complex comprising a target nucleic acid, said method comprising: a) adding a target nucleic acid to an array to form at least a first hybridization complex, said array comprising a solid support having a plurality of regions, each region comprising an electrode and a self-assembled mixed monolayer comprising i) blocking moieties, having a first end attached to said electrode, wherein said blocking moieties shield nucleic acids from said electrode; and ii) at least one modified nucleic acid comprising a nucleic acid and a linker moiety having a first and second end; wherein said first end of said linker is attached to said electrode and said second end is covalently attached to said nucleic acid; wherein at least two different regions comprise different probe nucleic acids; b) adding an agent that distinguishes between single and double stranded nucleic acids; and c) detecting the presence of said first hybridization complex.