Patent ID: 8153602

Claim:
A method for enhancing cellular uptake of an oligonucleotide administered as an aerosol into a lung of a mammal by including 2′-O-methoxyethyl and 5-methylcytosine modifications in said oligonucleotide, said method comprising: administering an aerosolized oligonucleotide into the lung of a mammal, wherein the aerosol particles have a size of about 1 to about 5 microns, wherein said oligonucleotide is 15 to 25 nucleotides in length, wherein at least 10 nucleosides in said oligonucleotide are 2′-O-methoxyethyl nucleosides, wherein each cytosine of said oligonucleotide is a 5-methylcytosine, and wherein said oligonucleotide is taken up by at least one cell type in the lung of the mammal.