Patent ID: 7767795

Claim:
A method for producing renatured, biologically active protein from a soluble denatured protein solution, said method comprising the steps of: (a) adjusting the concentration of denatured protein in solution to from about 0.01 mg/mL to about 500 mg/mL; (b) increasing pressure on the solution of denatured protein, which solution further comprises a chaotropic agent, to from about 0.25 kbar to about 3.5 kbar; and (c) incubating the solution of denatured protein under a pressure from about 0.25 kbar to about 3.3 kbar; then (d) reducing the chaotropic agent concentration to a level sufficient to permit biological activity of the protein at atmospheric pressure; then (e) after step (d), reducing the pressure to atmospheric pressure, whereby the protein has refolded to assume a native conformation and has biological activity of the native protein.