Patent ID: 8865434

Claim:
A method of producing saccharides containing glucose as the major constituent, comprising the steps of: providing, in a reaction tank: a pre-treated biomass substrate, which contains at least one selected from the group consisting of cellulose and hemicellulose, and lignin; and a solution, which includes a cellulose saccharifying enzyme; combining the substrate with the solution to prepare a mixture; after the step of combining, saccharifying in the reaction tank for 2 to 60 days at a temperature in the range from 50° C. to 60° C. the at least one selected from the group consisting of cellulose and hemicellulose with the cellulose saccharifying enzyme; providing stirring impellers in the reaction tank; and during the step of saccharifying, agitating the mixture with said stirring impellers at an agitation power Y (W/m 3 ) determined based on a substrate concentration X (w/v %) using a formula (1) below, Y ≦−0.0125 X 2 +1.195 X +23.25 (1) wherein the substrate concentration X (w/v %) is a value obtained by dividing a mass (g) of the at least one selected from the group consisting of cellulose and hemicellulose by a volume (mL) of the solution and multiplying by 100, Y is at least more than zero and X ranges from 5 to 50 w/v %, an amount of the lignin in the substrate is 15 wt % or less, a pH value of the mixture is 4 to 6, and protein concentration in the solution is 0.6 g/L or less.