Patent ID: 7611735

Claim:
A process for the preparation of a canola protein isolate having an increased proportion of 2S canola protein, which comprises: (a) providing an aqueous solution of 2S and 7S canola proteins consisting predominantly of 2S canola protein, said aqueous solution being in the form of concentrated supernatant from canola protein micelle formation and precipitation, wherein said canola protein micelle formation is effected by: (i) extracting canola oil seed meal at a temperature of at least 5° C. to cause solubilization of protein in said canola oil seed meal and to form an aqueous protein solution, (ii) separating said aqueous protein solution from residual oil seed meal, (iii) increasing the concentration of said aqueous protein solution to at least 200 g/L while maintaining the ionic strength substantially constant by a selective membrane technique to provide a concentrated protein solution, (iv) diluting said concentrated protein solution into chilled water having a temperature of below 15° C. to cause the formation of the protein micelles, and (v) separating supernatant from settled protein micellar mass, (b) heat treating said supernatant by heating the supernatant for about 5 to about 15 minutes at a temperature of about 75° to about 95° C. to cause precipitation of 7S canals protein, (c) removing precipitated 7S protein from the heat-treated supernatant, and (d) recovering from the supernatant resulting from step (c), which supernatant is a canola protein isolate having a protein content of at least 90 wt % (N×6.25) on a dry weight basis and having an increased proportion of 2S canola protein as compared to the aqueous solution of 2S and 7S canola proteins.