Patent ID: 7504494

Claim:
A multiplex PCR assay mixture for screening or detecting Mycobacterium tuberculosis, Toxoplasma gondii , pathogenically important fungi and cytomegalo virus (CMV) in a sample, comprising a combination of 4 sets of primers, one of said primer sets for the detection of Mycobacterium tuberculosis , a second of said primer sets for the detection of Toxoplasma gondii , a third primer set for the detection of pathogenically important fungi and a fourth primer set for the detection of CMV, said primers being compatible to each other, wherein the primer set for Mycobacterium tuberculosis is: MPB1: TCC GCT GCC AGT CGT CTT CC (SEQ ID NO:1) MPB2: GTC CTC GCG AGT CTA GGC CA (SEQ ID NO:2) for Toxoplasma gondii is: B1F: GGA ACT GCA TCC GTT CAT GAG (SEQ ID NO:3) B1R: TCT TTA AAG CGT TCG TGG TC (SEQ ID NO:4) for pathogenically important fungi is: B2F: ACT TTC GAT GGT AGG ATA G (SEQ ID NO:5) B4R: TGA TCG TCT TCG ATC CCC TA (SEQ ID NO:6) for Cytomegalo Virus (CMV) is: ACMVF: GTA CAC GCA CGC TGG TTA CC (SEQ ID NO:7) ACMVR: GTA GAA AGC CTC GAC ATC GC. (SEQ ID NO:8)