Patent ID: 8658037

Claim:
A method for treating a microbial community present in a treatment tank of a wastewater biological treatment facility, comprising the steps of: collecting the microbial community present in the treatment tank; staining the microbial community with a set of dyes consisting of a first fluorescent dye and a second fluorescent dye, wherein the first fluorescent dye which binds to a nucleotide chain of a microbe in the microbial community, and the second fluorescent dye is degraded by an enzyme in cells of the microbe to emit fluorescent light having a wavelength different from that of the first fluorescent dye; measuring a first fluorescent intensity (F 1 ) derived from the first fluorescent dye and a second fluorescent intensity (F 2 ) derived from the second fluorescent dye, calculating a value of a ratio (F 1 /F 2 ) of the first fluorescent intensity (F 1 ) to the second fluorescent intensity (F 2 ); performing, when the ratio (F 1 /F 2 ) calculated is less than 1 or more than 20, a wastewater treatment by either or both of: (A) placing a microbial preparation into a treatment tank, and (B) controlling a solids retention time of the treatment tank.