Patent ID: 8067194

Claim:
A method for the enzymatic hydrolysis of raw materials containing collagen and proteins and the separation of collagen from water soluble proteins, comprising the steps of: (1) enzymatically hydrolyzing the raw materials containing proteins and collagen in water to produce three layers comprising: (a) a top layer containing fat, (b) a mid-layer comprising water soluble constituents, water soluble proteins and denatured collagen, and (c) a non-soluble bottom layer comprising bones and non-soluble proteins; (2) separating said layers (a), (b) and (c); and (3) further separating said layer (b), comprising cooling said layer (b), without agitation, until said layer (b) reaches a temperature sufficient, and for a time period sufficient, for forming two layers, including a bottom layer (d) containing at least partially set collagen, and a liquid top layer (e) containing remaining water soluble proteins; (4) removing said layer (e); and (5) heating said layer (d) until said layer (d) becomes liquid.