Patent ID: 8168388

Claim:
A method of preparing nucleic acid template constructs for solid-phase nucleic acid amplification which comprises performing the following ligation reactions a) and b): a) a ligation reaction in which the first end of one or more target polynucleotide molecules of unknown sequence are ligated to surface-bound adaptor polynucleotide molecules; and b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules of unknown sequence; thereby producing one or more template constructs attached to a solid support, wherein each of said template constructs comprises a target polynucleotide molecule of unknown sequence ligated to a surface-bound adaptor polynucleotide molecule and a solution-phase polynucleotide molecule, and wherein the target polynucleotide molecules are not digested with a cleavage reagent prior to step a).