Patent ID: 7888489

Claim:
A nucleotide sequence comprising: a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, said synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; wherein the optimized codon frequency is such that at least 10% of the native codons have been exchanged with a synonymous codon, the synonymous codon changing the codon frequency such that the value of the absolute difference between the percentage for said synonymous codon in said frequency and the listed optimal percentage becomes smaller after modification, applying the following list of optimal percentages; cysteine by TGC (100%); phenylalanine by TTC (100%); histidine by CAC (100%); lysine by AAG (100%); asparagine by AAC (100%); glutamine by CAG (100%); tyrosine by TAC (100%); alanine is encoded by GCT (38%) GCC (51%), or GCG (11%); aspartate by GAC (64%); glutamate by GAG (74%); glycine by GGT (49%), GGC (35%), GGA (16%); isoleucine by ATT (27%), ATC (73%); leucine by TTG (13%), CTT (17%), CTC (38%), CTG (32%); proline by CCT (36%), CCC (64%); arginine by CGT (49%), CGC (51%); serine by TCT (21%), TCC (44%), TCG (14%), AGC (21%); threonine by ACT (30%), ACC (70%) and/or valine by GTT (27%), GTC (54%), GTG (19%); and optionally said nucleotide sequence comprises control sequences comprising: one translational termination sequence orientated in 5′ towards 3′ direction selected from the following list of sequences: TAAG, TAGA and TAAA, and/or one translational initiator coding sequence orientated in 5′ towards 3′ direction selected from the following list of sequences: GCTACCCCC; GCTACCTCC; GCTACCCTC; GCTACCTTC; GCTCCCCCC; GCTCCCTCC; GCTCCCCTC; GCTCCCTTC; GCTGCCCCC; GCTGCCTCC; GCTGCCCTC; GCTGCCTTC; GCTTCCCCC; GCTTCCTCC; GCTTCCCTC; and GCTTCCTTC (SEQ ID NO:21) TABLE 1 Optimal filamentous fungal codon frequency for synonymous codons in % .T. .C. .A. .G. T . . Phe Ser Tyr Cys . . T 0 21 0 0 T . . Phe Ser Tyr Cys . . C 100 44 100 100 T . . Leu Ser Stop Stop . . A 0 0 100 0 T . . Leu Ser Stop Trp . . G 13 14 0 100 C . . Leu Pro His Arg . . T 17 36 0 49 C . . Leu Pro His Arg . . C 38 64 100 51 C . . Leu Pro Gln Arg . . A 0 0 0 0 C . . Leu Pro Gln Arg . . G 32 0 100 0 A . . Ile Thr Asn Ser . . T 27 30 0 0 A . . Ile Thr Asn Ser . . C 73 70 100 21 A . . Ile Thr Lys Arg . . A 0 0 0 0 A . . Met Thr Lys Arg . . G 100 0 100 0 G . . Val Ala Asp Gly . . T 27 38 36 49 G . . Val Ala Asp Gly . . C 54 51 64 35 G . . Val Ala Glu Gly . . A 0 0 26 16 G . . Val Ala Glu Gly . . G 19 11 74 0.