Patent ID: 7947871

Claim:
A method of producing a transgenic strawberry plant comprising inoculation of tissue of a strawberry plant, with Agrobacterium thumefaciens which comprises at least one vector comprising at least one gene of interest wherein in the step of transformation a stage wise co-cultivation of explants is used which comprises the steps of: a) cutting leaves into individual leaf disks for preparing explants; b) cutting one or more narrow strips having a width not exceeding 2 mm from the leaf disks for the inoculation of the leaf disks; c) inoculating and co-cultivating the leaf disk obtained in step b) with the bacterial suspension and subsequently removing excess bacteria and incubating the leaf disk at 25-28° C. in darkness; d) forming first-stage explants having a width from 1 to 3 mm from the side of the first section of leaf disks by step b) incubating in darkness first-stage explants in a 23-25° C. and the leaf disks in a 25-28° C. e) forming from 2 to 5 next-stages explants having a width from 1 to 3 mm with a periodicity of 1 to 5 days by independent steps, selecting prepared explants that have a lowered frequency of necrotic reactions and f) allowing the selected explants to develop into a transgenic strawberry plant.