Patent ID: 8076154

Claim:
A method of calibration for an optical analysis instrument comprising: preparing a plurality of calibration solutions, each solution comprising a known concentration of a peracid and a known concentration of a peroxide; determining at least two parameters of an optical characteristic of each of the calibration solutions; determining, for at least one calibration solution, an estimated concentration of the peracid based on a first high order conversion equation, a first group of equation coefficients, and each of the at least two parameters of the optical characteristic; determining, for at least one calibration solution, an estimated concentration of the peroxide based on a second high order conversion equation, a second group of equation coefficients, and each of the at least two parameters of the optical characteristic; determining a difference between the known concentration of the peracid and the estimated concentration of the peracid, and a difference between the known concentration of the peroxide and the estimated concentration of the peroxide; and changing the first group of equation coefficients and the second group of equation coefficients so as to minimize the determined difference between the known concentration of the peracid and the estimated concentration of the peracid, and to minimize the determined difference between the known concentration of the peroxide and the estimated concentration of the peroxide wherein the plurality of calibration solutions comprises at least one of a set of solutions having a peracid concentration range from 1000 ppm to 4000 ppm, and a set of solutions having a peroxide concentration range from 5000 ppm to 40000 ppm.