Patent ID: 6936445

Claim:
An enzyme preparation comprising: (i) a styrene-divinylbenzene copolymer having fine pores with an average radius of 200-500 Å, (ii) an enzyme capable of asymmetrically hydrolyzing N-substituted C3-C4 cyclic imino-2-carboxylic acid ester to preferentially produce an (S)-N-substituted C3-C4 cyclic imino-2-carboxylic acid, and wherein said enzyme comprises any one of the following amino acid sequences (a) to (f): (a) an amino acid sequence as set forth in SEQ ID NO: 1; (b) an amino acid sequence as represented by the sequence corresponding to 35th to 255th amino acid in SEQ ID NO: 1; (c) an amino acid sequence as represented by the sequence corresponding to 79th to 255th amino acid in SEQ ID NO: 1; (d) an amino acid sequence encoded by a DNA, wherein 1) said DNA is a DNA that hybridizes with a DNA consisting of a nucleotide sequence coding for the amino acid sequence as set forth in SEQ ID NO: 1 at a high ion concentration of 6×SSC (900 mM NaCl, 90 mM sodium citrate) at a temperature of 65° C., and 2) the resulting hybrid is maintained after being incubated under a low ion concentration of 0.1×SSC (15 mM NaCl, 1.5 mM sodium citrate) at a temperature of 65° C. for 30 minutes; (e) an amino acid sequence that has sequence identity with the amino acid sequence as set forth in SEQ ID NO: 1 of 70% or greater; or (f) an amino acid sequence encoded by a nucleotide sequence having the restriction sites as shown in FIG. 1 .