Patent ID: 7011942

Claim:
A method of producing probes intended for labeling human chromosomes comprising mixing first amplification products and second amplification products obtained by two IRS-PCR amplifications from said chromosomes, labeling said amplification products with one or more fluorophore, and detecting said labeled amplification products, wherein said first amplification products are obtained using PCR primers specific for Alu and LINE DNA sequences and said second amplification products are obtained using PCR primers specific for Alu DNA sequences and said amplification products are labeled and detected with: (a) the fluorophore Cy3 having a maximum absorption wavelength of 554 nm and a maximum emission wavelength of 568 nm combined with an excitation filter of the 546DF10 type (Omega Optical) and with an emission filter of the 570DF10 type (Omega Optical); (b) the fluorophore TR having a maximum absorption wavelength of 593 nm and a maximum emission wavelength of 613 μm combined with an excitation filter of the 590DF10 type (Omega Optical) and with an emission filter of the 615DF10 type (Omega Optical); and (c) at least one fluorophore, absorption filter, and emission filter are selected from the group consisting of: (i) the fluorophore FITC having a maximum absorption wavelength of 494 nm and a maximum emission wavelength of 517 nm is coupled with the excitation filter of the 490DF30 type (Omega Optical) and with an emission filter of the 530DF30 type (Omega Optical); (ii) the fluorophore Cy5 having a maximum absorption wavelength of 652 nm and a maximum emissions wavelength of 670 nm combined with an excitation filter of the 650DF20 type (Omega Optical) and with an emission filter of the 670DF10 type (Omega Optical); (iii) the fluorophore Cy7 having a maximum absorption wavelength of 743 nm and a maximum emissions wavelength of 767 nm combined with an excitation filter of the 740DF25 type (Omega Optical) and with an emission filter of the 780EFLP type (Omega Optical); (iv) the fluorophore Cy5.5 having a maximum absorption wavelength of 675 nm and a maximum emission wavelength of 694 nm combined with an excitation filter of the 680DF20 type (Omega Optical) and with an emission filter of the 700EFLP type (Omega Optical); and (v) the fluorophore Bodipy 630/650 having a maximum absorption wavelength of 632 nm and a maximum emission wavelength of 658 nm used with an excitation filter of the 630DF20 type (Omega Optical) and with an emission filter of the 650EFLP type (Omega Optical).