Patent ID: 7384754

Claim:
A method for separating a deglycosylated protein comprising a) obtaining a mixture comprising a glycosylated protein and unglycosylated proteins, wherein the glycosylated protein comprises a protein having an O linked glycosylation site and a glycosyl group bound to the protein via the glycosylation site and has a molecular weight of 5000 daltons or less, b) contacting the mixture with a resin, wherein the resin comprises a nucleophile bound to a solid support via a linker, wherein said nucleophile is selected from the group consisting of amine, hydroxyl, sulfhydryl, and combinations thereof, said contacting done under conditions sufficient to remove the glycosyl group by β-elimination from the glycosylated protein to yield the deglycosylated protein having an unsaturated intermediate at the deglycosylation site, the deglycosylated protein bound to the solid support via the unsaturated intermediate at the deglycosylation site; c) rinsing the bound deglycosylated protein, thereby removing unglycosylated proteins; d) releasing the deglycosylated protein from the solid support.