Patent ID: 7582316

Claim:
A method of preparing a high yield Cissus quadrangularis keto-sterone composition from a C. quadrangularis plant comprising the steps of: washing, drying and grinding one or more roots of a C. quadrangularis plant to a root powder; adding a first solvent to the root powder of the C. quadrangularis plant to provide a first mixture of root extracts and the first solvent, wherein the first solvent is selected from the group consisting of toluene, methanol, ethanol, chloroform, ethyl acetate, ethylene dichloride and mixtures thereof; adding a second solvent to the first mixture to provide a second mixture comprising the first mixture and the second mixture, wherein substantially one or more keto-sterones of the first mixture is insoluble in the second solvent and a quantity of one or more impurities of the first mixture is soluble in the second solvent, and wherein the second solvent is a substantially non-polar solvent; separating a quantity of substantially one or more keto-sterones from the quantity of the one or more impurities of the second mixture; and combining a quantity of the substantially one or more keto-sterones with at least one physiologically acceptable carrier or excipient to produce the high yield C. quadrangularis keto-sterone composition having a predetermined keto-sterone content.