Patent ID: 8236751

Claim:
A method of increasing muscle tissue growth in a subject comprising: (a) administering to the subject a therapeutically effective amount of a Follistatin-Like Related Gene (FLRG) polynucleotide or polypeptide in the absence of other agents; (b) administering to a corresponding subject a specific inhibitor of myostatin expression or activity in combination with the FLRG polynucleotide or polypeptide; (c) comparing muscle tissue growth in the subject of (a) to muscle tissue growth of the corresponding subject of (b) to determine whether there is an increase in muscle tissue growth in the corresponding subject of (b) greater than that in the subject of (a); and (d) further administering to the subject of (a) a specific inhibitor of myostatin expression or activity wherein the muscle tissue mass in the corresponding subject of (b) is greater than that in the subject of (a), thereby increasing muscle tissue growth in the subject.