Patent ID: 7402432

Claim:
A method for producing T lymphocytes in vitro for administration to a patient, comprising: (a) removing a skin sample from said patient; (b) separately culturing keratinocytes and fibroblasts derived from said skin sample; (c) colonizing a support matrix with the cultured keratinocytes and fibroblasts of step (b); (d) obtaining hematopoietic precursor cells from the bone marrow of said patient; (e) adding said bone marrow hematopoietic precursor cells to the colonized matrix of step (c); (f) culturing said bone marrow hematopoietic precursor cells on said support matrix in the presence of one or more factors selected from the group consisting of IL-15; IL-7; and Flt-3 ligand, so as to induce production of T lymphocytes; and (g) harvesting T lymphocytes from said matrix.