Patent ID: 7601535

Claim:
A method for culturing animal cells, comprising: (a) providing an animal cell; (b) providing a cell culture medium at a temperature suitable for growing the animal cell of (a), wherein the medium comprises: 1) a total of from approximately 5.5 to 20 grams per liter of a plurality of amino acids; and 2) at least one carbohydrate; and (c) growing the animal cell of (a) in the medium of (b) at said growth temperature, wherein the utilization rate of the carbohydrate source as an energy source by the animal cell is altered as compared to the utilization rate in a conventional medium, thereby culturing cells, wherein the cell culture medium comprises the following amino acid components in about the following concentrations expressed as milligrams per liter (mg/L): Alanine 13.4; Arginine HCl 1,162.90; Asparagine-H2O 189.2; Aspartic acid 80; Cysteine HCl—H 2 O 105.4; Cystine-2HCl 105.4; Glutamic acid 79.4; Glutamine 1,997.2; Glycine 85.6; Histidine HCl—H 2 O 150.9; Hydroxyproline 63; Isoleucine 314.8; Leucine 330.6; Lysine HCl 394.6; Methionine 98.4; Phenylalanine 148.6; Proline 110.6; Serine 170.2; Threonine 221.6; Tryptophan 36.8; Tyrosine 174; and Valine 218; or the cell culture medium comprises the following amino acid components in about the following concentrations expressed as milligrams per liter (mg/L): Alanine 33.4; Arginine HCl 2,906.9; Asparagine-H2O 473.2; Aspartic acid 200; Cysteine HCl—H 2 O 105.4; Cystine-2HCl 421.4; Glutamic acid 198.4; Glutamine 4,993.2; Glycine 213.6; Histidine HCl-H 2 O 376.9; Hydroxyproline 158; Isoleucine 786.8; Leucine 826.6; Lysine HCl 986.6; Methionine 246.4; Phenylalanine 371.6; Proline 276.6; Serine 425.2; Threonine 553.6; Tryptophan 91.8; Tyrosine 435; and Valine 545.