Patent ID: 6884606

Claim:
A method of making lysine by fermentation, comprising culturing a strain of Corynebacterium glutamicum in a culture medium, said strain including a nucleic acid molecule encoding pyruvate carboxylase, said nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of: (a) a nucleotide sequence encoding the pyruvate carboxylase polypeptide having the amino acid sequence of SEQ ID NO: 2; (b) a nucleotide sequence encoding the pyruvate carboxylase polypeptide having the complete amino acid sequence encoded by the clone contained in ATCC Deposit No. PTA-982; and (c) a nucleotide sequence complementary to any of the nucleotide sequences in (a) or (b), wherein said pyruvate carboxylase is expressed 2 to 20 fold higher in said strain than in wild-type Corynebacterium glutamicum; producing lysine, and recovering said lysine.