Patent ID: 8658394

Claim:
A method of incorporating a non-canonical amino acid into a protein during a translation of said protein in bacterial cells, said method comprising: providing a modified ribosome in said bacterial cells, wherein said modified ribosome comprises a truncated L11 protein, wherein said truncated L11 protein in said modified ribosome is truncated at the first 72 amino acids of N-terminus of the L-11 protein; providing a nucleotide sequence in said bacterial cells, wherein said nucleotide sequence encodes said protein, and wherein said nucleotide sequence comprises an introduced stop codon at a site where said incorporation of said non-canonical amino acid into said protein is desired; providing a suppressor transfer RNA in said bacterial cells, wherein said suppressor transfer RNA is acylated with said non-canonical amino acid, and wherein the anti-codon of said suppressor transfer RNA recognizes said introduced stop codon in said nucleotide sequence; and wherein a translation of said transcript by said modified ribosome and said suppressor transfer RNA incorporates said non-canonical amino acid into said translated protein.