Patent ID: 8663957

Claim:
A non-naturally occurring microbial organism, wherein said microbial organism comprises exogenous nucleic acids encoding each of the following cyclohexanone pathway enzymes: (1) a PEP carboxykinase, (2) a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), (3) a 2-ketocyclohexane-1-carboxylate decarboxylase, and (4) an enzyme selected from the group consisting of (i) a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), (ii) a 2-ketocyclohexane-1-carboxyl-CoA transferase, and (iii) a 2-ketocyclohexane-1-carboxyl-CoA synthetase, wherein said cyclohexanone pathway enzymes are expressed in a sufficient amount to produce cyclohexanone.