Patent ID: 7955810

Claim:
A method to extract a composition comprising a plurality of species of interest and a lower concentration of a high abundance species of interest from a sample, comprising: providing a plurality of collectors capable of binding specifically with a sufficient binding affinity to a plurality of species of interest in a sample comprising at least one high abundance species of interest, wherein the quantity of a collector specific for the at least one high abundance species of interest is at least 50% less than the quantity of the at least one high abundance species of interest in the sample; placing the sample in contact with the plurality of collectors, whereby at least a portion of the plurality of collectors binds to the species of interest to generate bound species of interest, and at least 75% of the collector specific for the at least one high abundance species of interest binds to the at least one high abundance species of interest to generate bound high abundance species of interest; and separating the bound species of interest and the bound high abundance species of interest from the sample to generate an extracted composition, whereby the concentration of the at least one high abundance species of interest in the extracted composition is lower than the concentration of the at least one high abundance species of interest in the sample, wherein the species of interest is selected from the group consisting of a protein, protein fragment, peptide and combinations thereof, and the plurality of collectors comprise a collector type selected from the group consisting of a nucleic acid molecule, amino acid, peptide, protein, aptamer, antibody, plasmid, phage, cofactor, metal ion and combinations thereof.