Patent ID: 8741590

Claim:
An electrochemical biosensor having a layered structure, comprising: a supporting layer having at least one surface; an electrode in contact with the at least one surface of the supporting layer, the electrode including carbon; a sensing layer comprising an analyte-responsive enzyme; a biocompatible layer disposed over the sensing layer of the electrode; and an interferant eliminating layer comprising a redox enzyme; wherein at least a portion of the sensing layer and the biocompatible layer allow diffusion of an analyte therethrough; wherein the electrode is connectable with a device; and further wherein the electrode is a working electrode, the working electrode comprises the analyte-responsive enzyme and a mediator, at least one of the analyte-responsive enzyme and the mediator is chemically bonded to a polymer disposed on the working electrode, and at least one of the analyte-responsive enzyme and the mediator is crosslinked with the polymer.