Patent ID: 8008475

Claim:
A process for isolating nucleic acids comprising the following steps: charging a non-siliceous membrane from a given direction with nucleic acids, wherein said nonsiliceous membrane has two opposing sides; immobilizing the nucleic acids on one side of the non-siliceous membrane by binding the nucleic acids to said one side of the membrane in the presence of an immobilization buffer; releasing the immobilized nucleic acids from the non-siliceous membrane by applying an elution agent wherein the released nucleic acids do not pass through to the other side of the non-siliceous membrane; and removing the released nucleic acids from the same side of the non-siliceous membrane on which the nucleic acids were immobilized, wherein the released nucleic acids are removed without retrieving materials that have contacted the other side of said non-siliceous membrane, and wherein the membrane has pores that have a diameter of 1 μm to 50 μm.