Patent ID: 7524642

Claim:
A method of assaying for a candidate agent that modulates the attachment of a ubiquitin moiety to at least one ubiquitin agent, said method comprising: a) combining: i) a first ubiquitin agent, wherein the first ubiquitin agent is a ubiquitin activating agent; ii) a candidate agent; and iii) a ubiquitin moiety, wherein the ubiquitin moiety is selected from the group consisting of NEDD-8, SUMO-1, smt3a, smt3b and ISG-15; and b) assaying for the attachment of said ubiquitin moiety to said first ubiquitin agent, wherein a difference in ubiquitin attachment in the presence of said candidate agent as compared with in the absence of said candidate agent indicates that said candidate agent modulates the attachment of said ubiquitin moiety to at least one ubiquitin agent.