Patent ID: 8822152

Claim:
A method of performing a specific transcription-based amplification reaction of a selected first target nucleic acid in a sample, in a reaction wherein reagents specific for amplification of more than one target nucleic acid are present, comprising: (1) contacting the sample with at least a first oligonucleotide pair for amplifying a first target nucleic acid and a second oligonucleotide pair for amplifying a second nucleic acid target, (a) the first oligonucleotide pair consisting of a first promoter oligonucleotide having a 5′ end and a 3′ end and being non-blocked, the first promoter oligonucleotide comprising, at the 5′ end, an RNA polymerase promoter sequence, and 3′ of the RNA polymerase promoter sequence, a sequence complementary to a first target portion of the first target nucleic acid and a first primer oligonucleotide that is not blocked comprising a sequence homologous to a second target portion of the first target nucleic acid, which second target portion is 5′ of the first target portion of the first target nucleic acid, and (b) a second oligonucleotide pair consisting of a second promoter oligonucleotide having a 5′ end and a 3′ end, the second promoter oligonucleotide comprising, at the 5′ end, an RNA polymerase promoter sequence, and 3′ of the RNA polymerase promoter sequence, a sequence complementary to a first target portion of a second target nucleic acid wherein further, the second promoter oligonucleotide comprises, at the 3′ end, a blocking moiety such that extension therefrom is essentially prohibited and a second primer oligonucleotide that is not blocked comprising a sequence homologous to a second target portion of the second target nucleic acid, which second target portion is 5′ of the first target portion of the second target nucleic acid; and (2) providing selected reagents and conditions for transcription-based amplification, thereby performing a specific transcription-based amplification reaction of a selected first target nucleic acid in a sample, in a reaction wherein reagents specific for amplification of more than one target nucleic acid are present.