Patent ID: 8802025

Claim:
A reagent kit for analyzing a sample to measure basophils and/or nucleated erythrocytes in the sample comprising: a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid, which can lyse erythrocytes and damage a cell membrane of leukocytes so that a fluorescent dye can permeate therethrough, and a second reagent containing the fluorescent dye capable of staining nucleic acid wherein said cationic surfactant is at least one selected from the group consisting of a quaternary ammonium salt and a pyridinium salt, said nonionic surfactant is at least one selected from the group consisting of a polyoxyethylene alkyl ether, a polyoxyethylene polyoxypropylene alkyl ether, a polyoxyethylene castor oil, a polyoxyethylene hydrogenated castor oil, a polyoxyethylene sterol and a polyoxyethylene hydrogenated sterol, and said fluorescent dye is at least one dye selected from the group consisting of a fluorescent dye having the general formula (I): wherein: R 1 and R 2 are, the same or different from each other, an alkyl group having 1 to 6 carbon atoms; R 3 , R 4 , R 5 , and R 6 are, the same or different from each other, a hydrogen atom or an alkyl group; and X − is an anion; and a fluorescent dye having the general formula (II): wherein: R 7 and R 8 are, the same or different from each other, an alkyl group having 1 to 6 carbon atoms optionally containing an acidic group; and R 9 , R 10 , R 11 , and R 12 are, the same or different from each other, a hydrogen atom or an acidic group, provided that either one of R 7 to R 12 is/has an acidic group; and the acidic group which may be present in R 7 to R 12 may form a salt, provided that either one of the acidic group(s) which may be present in R 7 to R 12 is a group from which a proton has been liberated.