Patent ID: 7514241

Claim:
A method of detecting VT1 RNA comprising conducting NASBA (nucleic acid sequence-based amplification) or 3SR (self sustained sequence replication) on a sample suspected of containing VT1 RNA using the following primers: a first oligonucleotide consisting of SEQ ID NO: 2; and a second oligonucleotide consisting of SEQ ID NO: 15 and a RNA polymerase promoter sequence; which comprises: contacting VT1 RNA in a buffer suitable for nucleic acid amplification with a first oligonucleotide consisting of SEQ ID NO: 2, a second oligonucleotide consisting of SEQ ID NO: 15 and an RNA polymerase promoter, a cleavage oligonucleotide consisting of 5S (SEQ ID NO: 27) which may be chemically modified or aminated at its 3′ end, T7 RNA polymerase, and AMV-reverse transcriptase; and detecting amplified nucleic acids; wherein the presence or amount of amplified nucleic acids is indicative of the presence of VT1 RNA in said sample.