Patent ID: 8594947

Claim:
A method of screening for a modulator of ACE wherein the method comprises (a) obtaining an orthorhombic crystal of ACE or ACE-lisinnopril complex, wherein ACE consists of the amino acid sequence of SEQ ID NO: 2 expressed in mammalian cells and the crystal is in space group P2 1 2 1 2 1 with unit cell dimension of a=56.47, b=84.90 and c=133.99, (b) determining the three-dimensional structure of ACE using the crystal obtained in (a) by the X-ray diffraction method to produce the atomic coordinates in Table A or B, (c) constructing a three dimensional model of ACE utilizing the atomic coordinates of Table A or B, or portions thereof, (d) selecting or designing a putative modulator of ACE, and (e) determining the modulation of ACE by the modulator selected or designed in (d).