Patent ID: 7045361

Claim:
A biosensor molecule, the biosensor molecule comprising: an acridine-based fluorophore; a boronate binding moiety that is capable of specifically and reversibly binding a polyhydroxylate analyte in a sample; and a linker moiety that links the fluorophore to the boronate binding moiety, wherein the biosensor molecule is capable of emitting a light signal in the visible to near infrared region of the spectrum that can be correlated to a polyhydroxylate analyte concentration in a sample and further wherein the biosensor molecule comprises a molecule of the formula: wherein: F is an acridine-based fluorophore; R 1 is selected from the group consisting of hydrogen, aliphatic and aromatic moieties; R 2 and R 4 are optional moieties selected from the group consisting of hydrogen, aliphatic and aromatic moieties and groups that are capable of forming covalent bonds to a biocompatible matrix; L 1 and L 2 are —CH 2 —; Z is nitrogen; R 3 is an optional group selected from the group consisting of hydrogen, aliphatic and aromatic moieties and groups that are capable of forming covalent bonds to a biocompatible matrix; and wherein F and Z are capable of being involved in an intramolecular photo-induced electron transfer process that quenches the fluorescence of F in the absence of bound polyhydroxylate analyte.