Patent ID: 7341871

Claim:
A method of expressing a nucleic acid sequence of interest in a mammalian cell, comprising: a) providing: i) an isolated mammalian cell selected from the group consisting of (a) a liver cell, and (b) a mouse cell selected from the group consisting of an embryonic stem cell, an eight-cell embryo cell, a blastomere cell, a blastocoele cell, and a fertilized egg cell; ii) a nucleic acid sequence of interest heterologous to SEQ ID NO:3; iii) a promoter sequence, and iv) one or more age regulatory sequences selected from SEQ ID NO:3 and a functional portion of SEQ ID NO:3; b) operably linking said nucleic acid sequence of interest, said promoter sequence, and said one or more age regulatory sequences to produce a transgene, wherein said age regulatory sequence comprised in said transgene is located at a position selected from the group consisting of the 3′ end of said promoter sequence and the 3′ end of said nucleic acid sequence of interest; and c) introducing said transgene into said mammalian cell under conditions such that said nucleic acid sequence of interest is expressed in said mammalian cell.