Patent ID: 8389485

Claim:
A method of reducing or inhibiting inflammation in a subject having an inflammatory disease or disorder comprising, administering to the subject a siRNA-delivery system, comprising yeast cell wall particles (YCWPs), wherein the YCWPs individually comprise a yeast cell wall particle (YCWP) exterior and a multilayered nanoparticle interior, wherein the multilayered nanoparticle interior comprises a cationic nucleic acid core, coated with at least one siRNA layer, wherein the siRNA layer is coated with at least one cationic trapping layer, wherein the multilayered nanoparticle interior is produced by first introducing into the YCWP exterior, components to form the cationic nucleic acid core such that the core forms inside the YCWP exterior, followed by introducing siRNA such that the siRNA layer forms on the surface of the core, followed by introducing a trapping molecule such that the trapping layer forms on the siRNA layer, and wherein the siRNA targets a gene involved in inflammation, wherein the siRNA is present at a ratio of about 0.1×10 −7 to 100×10- 7 pmol siRNA per YCWP, wherein the siRNA-delivery system is administered under conditions facilitating delivery of the siRNA to phagocytic cells of the subject, such that inflammation is reduced or inhibited in the subject having the inflammatory disease or disorder.