Patent ID: 7935488

Claim:
A method of amplifying a restriction fragment from at least one source of DNA comprising: (a) digesting said source of DNA with at least one restriction endonuclease that cleaves the DNA at its recognized target site to produce restriction fragments; (b) ligating in molar excess to the restriction fragments at least one adaptor to the ends of at least one restriction fragment, wherein each adaptor comprises (i) a first end that is designed to ligate to said restriction fragment; (ii) a second end that is designed so that it cannot ligate to said restriction fragment, and (iii) situated between said first and second ends: (1) a specific primer binding site, and (2) different unique nucleotide sequences at specific positions within said adaptor; and (c) amplifying said tagged restriction fragment with primers that bind only to the specific primer binding site in said adaptor to produce amplified restriction fragments from said source of DNA, wherein the source of DNA correlates to at least one of the different unique sequences positioned in said adaptor.