Patent ID: 7163917

Claim:
A method of synthesizing alanylglutamine, comprising the steps of: 1) form active ester by the reaction of 10 mmol N-terminal protected alanine, 10 to 30 mmol of triphenylphosphine and 10 to 30 mmol of hexachloroethane, in organic solvent for 20 minutes to 3 hours, and reaction temperature is −5 to 30° C.; 2) react the active ester obtained from step 1) with 10 to 30 mmol of glutamine to form N-terminal protected alanylglutamine, in a liqiuid mixture made by organic solvent and aqueous solution of inorganic base, wherein the volume ratio of organic solvent and aqueous solution of inorganic base is 0–4, reaction temperature is −5 to 30° C. and the pH is controlled at 8.5 to 13; 3) acidify the reaction mixture of step 2) with inorganic acid to pH <3.0; and 4) remove the N-terminal protecting group to obtain alanylglutamine.