Patent ID: 8182841

Claim:
A method for reducing inflammation in a subject in need thereof comprising, administering to the subject a pharmaceutical formulation comprising: (a) an AM preparation prepared from pulverized or around amniotic membrane isolated from frozen or previously frozen placenta comprising: (i) high molecular weight hyaluronan (HA) that is cross-linked by a covalent bond to the heavy chain of inter-α-trypsin inhibitor (IαI), the high molecular weight HA having a molecular weight greater than 1000 kDa; (ii) tumor necrosis factor-stimulated gene 6 (TSG-6); (iii) pentraxin (PTX-3); and (iv) thrombospondin (TSP-1); and (b) a pharmaceutically acceptable diluent, excipient, or carrier; wherein the ratio of total protein to HA in the formulation is less than 100 parts total protein to 1 part HA; and wherein inflammation is reduced.