Patent ID: 7056742

Claim:
A method for producing hydroquinone glucoside in a green plant comprising: a) growing a green plant under suitable conditions, the green plant having i) an endogenous source of chorismate; ii) a nucleic acid fragment encoding active para-hydroxybenzoic acid 1-hydroxylase enzyme at least 95% identical to the amino acid sequence set forth in SEQ ID NO:23; and iii) an endogenous source of hydroquinone glucosyltransferase; and iv) a chorismate pyruvate lyase expression cassette having the structure: P-T-C-D-CPL wherein P is a promoter suitable for driving the expression of a chorismate pyruvate lyase gene; T is a nucleic acid molecule encoding a Rubisco chloroplast transit peptide; C is a nucleic acid molecule encoding a Rubisco chloroplast transit peptide cleavage site; and D is a nucleic acid molecule encoding four contiguous amino acids of the N-terminal portion of a Rubisco chloroplast transit peptide donor polypeptide; CPL is a nucleic acid molecule encoding chorismate pyruvate lyase enzyme having the amino acid sequence set forth in SEQ ID NO:30; and P, T, C, D, and CPL are operably linked with each other; and b) recovering the hydroquinone glucoside produced in step a).