Patent ID: 7319520

Claim:
A method for determining illumination and detection parameters in a spectral scanning microscope for data acquisition comprising: generating light from at least one laser that emits light of one wavelength and converting the light of one wavelength into the light comprising a discrete or continuous wavelength region; calculating a band of the illuminating light for each fluorescent dye present in the specimen and calculating a band of a detected light for each fluorescent dye present in the specimen by using the excitation and emission spectra stored in a database; performing data acquisition with the spectral scanning microscope using for each fluorescent dye in the specimen its calculated band of the illuminating light within the wavelength region and its calculated band of a detected light, wherein the emission spectra s i em of the fluorescent dyes in the specimen are grouped into a mixed detection matrix M Det ; wherein the excitation spectra s i ex of the fluorescent dyes in the specimen are grouped into a mixed excitation matrix M Ex ; wherein the mixed excitation matrix M Ex and the mixed detection matrix M Det model illumination conditions of the specimen, when a spectral composition of the illuminating light is known and a setting of a mirror stop arrangement is made in consideration of the fluorescent dyes present in the specimen.