Patent ID: 8133670

Claim:
A method for making a population of nucleic acid molecules, the method comprising: (a) harvesting a population of synthesized single-stranded nucleic acid molecules that are covalently attached to a microarray, wherein each of the synthesized nucleic acid molecules consists of a predetermined nucleic acid sequence consisting of: (i) a 5′ primer binding region, (ii) a 3′ primer binding region, (iii) one of a number of different target sequences located between the 5′ and 3′ primer binding regions, and additionally, (iv) one of a number of unique target identifier sequences, wherein the target identifier sequence is not comprised within the target sequence or primer binding regions, and wherein each unique target identifier sequence is associated with only one particular target sequence; (b) amplifying the population of synthesized single-stranded nucleic acid molecules harvested in step (a) to generate a population of double-stranded DNA molecules, and (c) introducing the double-stranded DNA molecules into vector molecules.