Patent ID: 7244815

Claim:
A method for preparing an immobilized peptide derivative of formula (I): Sub-[L]-[N(R 1 )-A-C(O)]—[NH-A-C(O)] n+m —OH (I) comprising: (a) reacting an immobilized compound of formula (II); Sub -(L)-[N(R 1 )-A-C(O)]—[NH-A-C(O)] n —OH (II) with an amino acid ester or peptide ester derivative of formula (III): H—[NH-A-C(O)] m —O(tBu) (III) in the presence of a coupling agent to yield an immobilized peptide derivative of general formula (IV): Sub-[L]-[N(R 1 )-A-C(O)]—[NH-A-C(O)] n+m —O(tBu) (IV); (b) removing the tBu (t-butyl) group to produce the immobilized peptide derivative of general formula (I); wherein n is a positive integer, m is a positive integer, Sub is a solid support, L is a cleavable linker to the amino acid or peptide derivative, each NH-A-C(O) independently is the residue of a naturally occurring or a synthetic amino acid including a sidechain-protected amino acid or a peptide composed thereof; or the structure —NH-A represents a heterocyclic group, and R 1 is a removable amine protecting group or an irreversible N-capping group.