Patent ID: 8236943

Claim:
A nucleic acid-lipid particle comprising: (a) an siRNA that silences Apolipoprotein B (APOB) expression, wherein the siRNA consists of the following sense and antisense strand sequences: 5′-AGUGUCAUCACACUGAAUACC-3′ (SEQ ID NO:4) 3′-GUUCACAGUAGUGUGACUUAU-5′ (SEQ ID NO:11), wherein the bolded nucleotides are 2′OMe nucleotides; (b) a cationic lipid having the following structure: or salts thereof, wherein: R 1 and R 2 are either the same or different and are independently optionally substituted C 12 -C 24 alkyl, optionally substituted C 12 -C 24 alkenyl, optionally substituted C 12 -C 24 alkynyl, or optionally substituted C 12 -C 24 acyl; R 3 and R 4 are either the same or different and are independently optionally substituted C 1 -C 6 alkyl, optionally substituted C 2 -C 6 alkenyl, or optionally substituted C 2 -C 6 alkynyl or R 3 and R 4 may join to form an optionally substituted heterocyclic ring of 4 to 6 carbon atoms and 1 or 2 heteroatoms chosen from nitrogen and oxygen; R 5 is either absent or hydrogen or C 1 -C 6 alkyl to provide a quaternary amine; m, n and p are either the same or different and are independently either 0, 1 or 2, with the proviso that m, n, and p are not simultaneously 0; q is 0, 1, 2, 3, or 4; Y and Z are either the same or different and are independently O, S, or NH; and (c) a non-cationic lipid.