Patent ID: 8617850

Claim:
A primer pair capable of amplifying a portion of a CAstV genome, wherein the primer pair comprises at least one oligonucleotide primer sequence that has binding specificity to a precapsid region of the CAstV genome; wherein the precapsid region is defined as a 106 nucleotide region located at the 3â€² end of the polymerase gene, wherein the 106 nucleotide region comprises an intergenic region between the polymerase gene (orf1b) and the capsid gene (orf2) and the first four nucleotides of the capsid gene, and wherein the precapsid region has a polynucleotide sequence TGCGTCGAGGAGTATGCCGCTGCTGAAGAAATACAGTTACCAGAAGTCGGGC CCGACTTCTTTCAGAAAATCTGGTAGAGGGATGGACCGAAATATAGCA GCATGG (SEQ ID NO: 8); and wherein the primer pair comprises oligonucleotide primer sequences: (SEQâ€ƒIDâ€ƒNO:â€ƒ1) forwardâ€ƒprimerâ€ƒGCYGCTGCTGAAGAWATACAG, and (SEQâ€ƒIDâ€ƒNO:â€ƒ3) reverseâ€ƒprimerâ€ƒCATCCCTCTACCAGATTTTCTGAAA.