Patent ID: 7494811

Claim:
A method of growing new bone or bone tissue under in vitro cell culture conditions comprising, providing a bioreactor, ground demineralized bone, nondemineralized bone, and bone-forming cells, wherein the nondemineralized bone comprises at least one of nondemineralized bone fibers and nondemineralized bone particles, placing the ground demineralized bone, nondemineralized bone, and the bone-forming cells in the bioreactor, and providing a nutrient solution comprising nutrients and oxygen for the bone-forming cells, causing the nutrient solution to flow into, through, and out of the bioreactor containing the ground demineralized bone, nondemineralized bone, and the bone-forming cells to bring the flowing nutrient solution into contact with the bone-forming cells, wherein nutrients and oxygen in the flowing nutrient solution are at sufficient concentrations to permit differentiation of the bone-forming cells into bone or bone tissue in the bioreactor.