Patent ID: 8530170

Claim:
A method for detecting a target analyte in a test sample, said method comprising: (a) providing a solid support comprising an electrode comprising: (i) a self-assembled monolayer (SAM); (ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex, a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E 0 when said self-immolative moiety is present; (iii) a capture binding ligand that binds said analyte; (b) contacting said target analyte and said solid support, under conditions wherein said target analyte binds said capture binding ligand to form a first complex; (c) contacting said first complex with a soluble capture ligand that binds said target analyte, wherein said soluble capture ligand comprises a peroxide generating moiety to form a second complex; (d) adding a peroxide substrate to said second complex under conditions such that peroxide is generated and said self-immolative moiety is removed such that said EAM has a second E 0 when said self-immolative moiety is absent; and (e) detecting said second E 0 as an indication of the presence of said target.