Patent ID: 8507758

Claim:
A method for producing a transgenic plant comprising a nucleotide sequence of interest inserted into its genome, where said method consists essentially of: a) transforming a plant tissue or plant cell with said nucleotide sequence of interest, which nucleotide sequence of interest comprises a gene of interest; wherein said plant tissue or plant cell is not transformed with an ancillary nucleotide sequence comprising a reporter sequence or a selectable marker sequence; b) regenerating said plant tissue or plant cell without selection for a reporter or selectable marker following said transforming, to produce a transgenic plant; and c) directly detecting the presence or absence in said transgenic plant of said nucleotide sequence of interest, an encoded product of said nucleotide sequence of interest, or a metabolic product of said encoded product; wherein said transgenic plant comprises said nucleotide sequence of interest introduced by said transforming; wherein said transforming does not introduce a transposable element into said plant tissue or plant cell; and wherein said nucleotide sequence of interest encodes one or more products selected from the group consisting of a hybrid Cry1Ac-1Acf Bacillus thuringiensis (Bt) protein, a Cry1Bb Bt protein, and a Cry1Ac Bt protein.