Patent ID: 8008085

Claim:
A method of measuring HbA1c, wherein the method comprises: (A) collecting a hemoglobin-containing material from a subject and storing the collected hemoglobin-containing material for a period of at least one day; (B) reducing carbon dioxide bonded to hemoglobin in the hemoglobin-containing material stored in (A) so as to produce a hemoglobin-containing sample; (C) cleaving the hemoglobin in the hemoglobin-containing sample produced in (B) by applying a protease treatment to the hemoglobin-containing sample; (D) treating a glycated part of a hemoglobin fragment obtained from cleaving the hemoglobin in the hemoglobin-containing sample in (C) with fructosyl amine oxidase; and (E) determining an amount of HbA1c in the hemoglobin-containing sample by measuring a redox reaction between the glycated part and the fructosyl amine oxidase and calculating the amount of HbA1c based on a measurement of the redox reaction.