Patent ID: 7230156

Claim:
A method for preparing a sterile transgenic carp comprising: (i) constructing antisense RNA expression vector comprising a backbone vector, a carp β-actin gene promoter comprising SEQ ID NO: 1, a carp salmon-type gonadotropin-releasing hormone gene comprising a nucleotide sequence encoding SEQ ID NO: 2 and a 3′flanking sequence of grass carp growth hormone gene, comprising SEQ ID NO: 3, where the carp salmon-type gonadotropin-releasing hormone gene operably linked to the carp β-actin gene promoter in a 3′–5′direction; (ii) preparing a transgenic carp by injecting the antisense RNA expression vector into a carp embryo; (iii) screening resulting transgenic carp by Polymerase Chain Reaction to determine carp positive for the transgene and determining serum GtH concentration of positive carp by RIA; (iv) comparing the serum GtH concentration of positive carp; and (v) identifying sterile carp whose genome comprises the antisense RNA expression vector.