Patent ID: 6946241

Claim:
A physiological medium which comprises an aqueous solution in sterile purified water of: (i) a salt component comprising: (a) from 100 to 150 mmoles/L of sodium ions, (b) from 2.5 to 6.2 mmoles/L of potassium ions, (c) from 0.1 to 2.5 mmoles/L of calcium ions, (d) from 0.4 to 25 mmoles/L of magnesium ions, and (e) from 96 to 126 mmoles/L of chloride ions; (ii) a buffer component comprising (f) from 21 to 27 mmoles/L of bicarbonate ions, and (g) from 1 to 12 mmoles/L of TES, MOPS or BES; (iii) a substrate component comprising: (h) 2 to 11 mmoles/L of glucose (i) 50 to 150 μmoles/L of glycerol and (j) 7 to 15 μmoles/L of choline; (iv) an amino acid component comprising: (k) 5 to 400 μmoles/L of glutamate, (l) 5 to 200 μmoles/L of aspartate and (m) 100 to 2000 μmoles/L of glutamine; (v) a co-enzyme component comprising: (n) 1 to 120 nmoles/L of thiamine pyrophosphate; (vi) a vitaminoid component comprising: (o) 40 to 70 μmoles/L of D- or DL- or L-carnitine; (vii) a protein component comprising: (p) 5 to 200 m I.U./L of porcine or human insulin.