Patent ID: 7130746

Claim:
A computer system for determining a level of protein activity in a cell of a cell type comprising a processor and a memory coupled to said processor, said memory encoding one or more programs, said one or more programs causing said processor to perform a method comprising (i) receiving perturbation response profiles of said protein for said cell type, wherein each said perturbation response profile comprises measurements of a plurality of cellular constituents in a first cell of said cell type at one of a plurality of discrete levels of perturbation to said protein, (ii) interpolating measurements of each cellular constituent of said plurality in said perturbation response profiles over said plurality of discrete levels of said perturbation to obtain a perturbation response curve of measurements of said cellular constituent as a function of level of said perturbation to said protein so that an interpolated perturbation response profile comprising measurements of said plurality of cellular constituents may be extracted at any level over a range of levels of perturbation to said protein, and (iii) determining a level of perturbation to said protein at which similarity is greatest between a diagnostic profile and an interpolated perturbation response profile, said diagnostic profile comprising measurements of said plurality of cellular constituents in a second cell of said cell type, said interpolated perturbation response profile comprising measurements of said plurality of cellular constituents extracted for said determined level from said perturbation response curves; wherein said determined level of perturbation to said protein represents said protein activity level in said cell type.