Patent ID: 7847072

Claim:
A process for obtaining osteogenic proteins from mammalian bone tissue comprising: i) contacting bone tissue with an acidic demineralization medium to provide demineralized bone tissue and a mineral-containing supernatant; ii) separating the mineral-containing supernatant from the demineralized bone tissue; and iii) recovering osteogenic proteins from the mineral-containing supernatant, wherein said recovering comprises removing at least part of the mineral component of the mineral-containing supernatant to provide a protein supernatant solution; extracting osteogenic proteins from the protein supernatant solution by contacting the protein supernatant solution with a protein extraction agent to provide an extracted protein medium; filtering the extracted protein medium in a first ultrafiltration step using a first ultrafiltration membrane having a nominal molecular weight cutoff corresponding to a high molecular weight limit to provide a permeate comprising a first osteogenic solution; filtering the first osteogenic solution in a second ultrafiltration step using a second ultrafiltration membrane having a nominal molecular weight cutoff corresponding to a low molecular weight limit to provide a retentate comprising a second osteogenic solution; and purifying the osteogenic proteins in the second osteogenic solution, wherein the first or the second ultrafiltration step comprises tangential flow filtration.