Patent ID: 8512649

Claim:
A method for performing a kinetic assay, the method comprising the steps of: attaching a fluid handling system to a device, where the fluid handling system includes a first fluid delivery tip, a second fluid delivery tip, and a fluid removal tip all of which are connected to the device, wherein the device has a body including: a first fluid inlet connected to the first fluid delivery tip; a second fluid inlet connected to the second fluid delivery tip; and a fluid outlet connected to the fluid removal tip; and wherein the body has a flow chamber formed therein, wherein the flow chamber including: a first flow restrictor associated with the first fluid inlet; a second flow restrictor associated with the second fluid inlet; a central portion having one corner of one end associated with the first flow restrictor and another corner of the one end associated with the second flow restrictor; the central portion having a sensing surface on which there is immobilized a target; and an outlet portion associated with the fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the one end of the central portion; flowing a buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while an inspection system obtains a baseline measurement; stopping the flow of the buffer solution; flowing an analyte solution through the second fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains an association measurement; stopping the flow of the analyte solution; and flowing the buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains a disassociation measurement.