Patent ID: 8221994

Claim:
An immunosensor, comprising: a lower electrode having a first liquid reagent comprising an antibody conjugated to an enzyme in a buffer, and a second liquid reagent comprising ferricyanide, a substrate for the enzyme, and an electrochemical mediator in a dilute acid solution, the first and second liquid reagents being striped on the lower electrode and then dried; an upper electrode having magnetic beads conjugated to an antigen striped and dried thereon; a separator disposed between the lower and upper electrodes; a reaction chamber formed in the separator and having the first reagent and the magnetic beads conjugated to the antigen disposed therein; a detection chamber formed in the separator and having the second reagent disposed therein; a fill chamber formed at least partially in the separator and one of the lower and upper electrodes, spaced a distance apart from the detection chamber, and overlapping at least a portion of the reaction chamber; a vent formed at least partially in each of the separator, the lower electrode, and the upper electrode, spaced a distance apart from the reaction chamber, and overlapping at least a portion of the detection chamber; a hydrophilic adhesive tape having an incorporated anticoagulant coupled to one of the lower and upper electrodes, disposed over the vent, and configured to form a wall of the fill chamber and seal the vent; and a sealing component coupled to the other of the lower and upper electrodes, disposed over the vent, and configured to seal the vent.