Patent ID: 8193518

Claim:
A method for locating at least N (N>1) fluorophores (F 1 , F 2 ) in a diffusing medium using at least one source (S 1 -S 4 , L 1 -L 4 ) of pulsed radiation capable of emitting radiation to excite this fluorophore, and at least one detector (D 1 -D 5 ) capable of measuring a fluorescence signal emitted by this fluorophore comprising: illumination of the medium by a radiation source, detection, by at least one detector, of the signal produced by the medium at the fluorescence wavelength, for at least one source-detector pair, performing a temporal distribution M sd (t) of the signal received by the detector, the diffusing medium being discretized into M voxels, each fluorophore occupying one voxel among the M voxels of the medium, according to a distribution m, the computing of at least one basic parameter χ N i,m , which, for at least one of said temporal distributions M sd (t), combines at least one magnitude obtained from at least one moment of said distribution, and at least one estimation of this magnitude.