Patent ID: 7927796

Claim:
A method of genotyping a nucleic acid sample using pairs of solid phase carriers, where members of a particular pair display a probe having a subsequence complementary to one of two different target subsequences, wherein said two different target subsequences differ from each other at one position and wherein at least some of said target subsequences, individually or together, are present in a nucleic acid sample amplified from a eukaryotic organism, and where the number of members displaying each said probe subsequence is known, comprising: reacting the nucleic acid sample with the members of the pairs of solid phase carriers such that a positive signal is generated upon reaction of a target subsequence with its complementary probe; and determining whether the target nucleic acid sample is homozygous for either of the subsequences, or is heterozygous by applying the formulas below: where =n c v equals the number of carriers carrying the probe complementary to one of two different target subsequences, and n c w equals the number of carriers carrying the probe complementary to the other of said two different target subsequences, and n c + is the total number of the carriers which carry a positive-signal following said reaction, then: when n c +/n c w ≈1; the sample is homozygous for the one subsequence (designated W); when n c +/n c v ≈1; the sample is homozygous for the other subsequence (designated V); and when n c +/(n c v +n c w )≈1; the sample is heterozygous.