Patent ID: 7303879

Claim:
A method for determining a rate of single nucleotide polymorphism (SNP) at a first location genomic DNA of a plurality of organisms, comprising: providing genomic DNA of each of a plurality of different organisms, the genomic DNA of each organism comprising first and second portions; preparing, from the genomic DNA of each organism, first and second amplicons, for each organism (i) the first amplicon corresponding to the first portion of the genomic DNA and (ii) the second amplicon corresponding to the second portion of the genomic DNA; preparing, from the first and second amplicons of the genomic DNA of each organism, a plurality of duplexes, at least some of the duplexes comprising (i) a portion of one of the first amplicons and (ii) a portion of one of the second amplicons; and subjecting the duplexes to temperature gradient electrophoresis to obtain first electrophoresis data indicative of the rate of SNP at the first location in the genomic DNA of the plurality of organisms.