Patent ID: 8198390

Claim:
A method for fabricating a biosensor, comprising: immersing a substrate in an aqueous solution comprising a water soluble functional polyethylene glycol-grafted polysiloxane polymer comprising a polysiloxane backbone and functional polyethylene glycol side chains, the polymer having a general formula: wherein A is selected from the group consisting of hydrogen, methyl, and methoxy, B is a functional group capable of binding to a biologically-sensitive material and is selected from the group consisting of a carboxyl group, an amino group, an aldehyde, a sulfohydryl, a hydroxyl group, a maleimide, a nitriloacetic acid, a haloacetyl, a cyano, a hydrazide, an epoxy, an aziridine, a sulfonylchloride, a trifluoromethyldiaziridine, a pyridylsulfide, a N-acetyl-imidazole, a vinylsulfone, an arylazide, a diazoacetate, a benzophenone, an isothiocyanate, an isocyanate, an imidoester, a fluorobenzene, a phenyl, a benzenesulfonamide, a dinitrophenyl, a triphenymethyl, a hydroquinone, a biotin, and a short peptide, D is a functional group capable of binding to a substrate and is selected from the group consisting of CH 3 —CH 2 —S—S—, a 5-(1,2-dithiolan-3-yl)pentanoic acid ester, a cyclic disulfide within a 5-membered ring with general formula: a TT′T″Si-group, a TT′RSi-group, and a TRR′Si-group, wherein T, T′ and T″ are each independently methoxy or ethoxy groups and wherein R and R′ are independently alkyl groups with a formula —(CH 2 )n′-CH 3 , wherein n′ is an integer of from 0 to 3, and m is an integer from 3 to 5, v is an integer from 0 to 5, w is an integer from 4 to 11, x is an integer from 0 to 35, z is an integer from 1 to 33, x+y+z is an integer from 8 to 40, n is an integer from 8 to 30, and y is an integer from 7 to 35, wherein the immersing is conducted in an absence of toluene, whereby a layer of the functional polyethylene glycol-grafted polysiloxane polymer is deposited on the substrate, wherein the substrate with deposited functional polyethylene glycol-grafted polymer comprises a component of a biosensor.