Patent ID: 8343743

Claim:
An isolated recombinant ubiquitin ligase molecule that comprises: a. a toxin binding domain that has an affinity for an enzymatically active fragment of one or more toxins or toxin serotypes, wherein the toxin binding domain comprises a non-cleavable SNAP-25 domain that is encoded by a nucleic acid molecule that consists essentially of a nucleic acid sequence selected from the group consisting of: i. a nucleic acid sequence having greater than or equal to about 90% identity with nucleic acid bases 1 to 618 of SEQ ID NO: 11 or 13; ii. a nucleic acid sequence complement having greater than or equal to about 90% identity with nucleic acid bases 1 to 618 of SEQ ID NO: 11 or 13; iii. a nucleic acid molecule that hybridizes to nucleic acid bases 1 to 618 of SEQ ID NO: 11 or 13 under high stringency conditions, wherein said conditions comprise a temperature range between about 40° C. and about 60° C., an SSC concentration range between about 1× and about 10× and a reaction time range of between about 30 seconds and about 36 hours; and iv. a nucleic acid sequence that encodes an amino acid sequence having greater than or equal to about 90% similarity to a sequence set forth in amino acid bases 1 to 206 of SEQ ID NO: 12 or 14; and b. an E3-ligase domain that comprises an E3-ligase or polypeptide that facilitates E2-mediated ubiquitination of the toxin enzymatically active fragment, wherein the E3-ligase domain is encoded by a nucleic acid molecule that consists essentially of a nucleic acid sequence selected from the group consisting of: i. a nucleic acid sequence having greater than or equal to about 90% identity with nucleic acid bases from 619 to 1029 of SEQ ID NO: 11 or 13; ii. a nucleic acid sequence complement having greater than or equal to about 90% identity with nucleic acid bases from 619 to 1029 of SEQ ID NO: 11 or 13; iii. a nucleic acid molecule that hybridizes to nucleic acid bases from 619 to 1029 of SEQ ID NO: 11 or 13 under high stringency conditions, wherein said conditions comprise a temperature range between about 40° C. and about 60° C., an SSC concentration range between about 1× and about 10× and a reaction time range of between about 30 seconds and about 36 hours; and iv. a nucleic acid sequence that encodes an amino acid sequence having greater than or equal to about 90% similarity to a sequence set forth in amino acid bases 207 to 343 of SEQ ID NO: 12 or 14.