Patent ID: 8580570

Claim:
A process for carrying out a chemical analysis method on a sample, said method comprising the steps of: providing a porous layer open tube (PLOT) capillary column, or channel in a microfabricated device, said column or channel comprising: a capillary column or channel having an i.d. of 15 μm or less, an entrance end and an exit end; a rigid porous layer separation medium comprising a highly crosslinked, macroporous, organic polymeric stationary phase layer covalently attached to the inner wall surface of said column or channel, wherein said organic polymeric stationary phase layer is from 0.5-3 μm in thickness, and wherein the reproducibility of retention time on comparable said columns or channels during use in high performance liquid chromatography varies less than 10%; and an open unfilled bore inside said rigid porous layer, wherein said open bore is disposed around a central axis of said column or channel and extends the length of said column or channel; coupling a mass flow or concentration sensitive detector via an interface to the exit end of said column or channel, wherein said interface is an electrospray ionization (ESI) interface or a matrix assisted laser desorption ionization (MALDI) interface; applying an aliquot of a liquid sample to said column or channel; conducting a high performance liquid chromatographic separation procedure on said applied sample; and detecting said separated sample with said detector to carry out said chemical analysis method.