Patent ID: 7153659

Claim:
A method for determining a CD8+ T-cell epitope in a protein, comprising the steps at: (a) obtaining from a solution of dendritic cells and a solution of naive CD8+ T-cells from a single human blood source; (b) differentiating said dendritic cells, in said solution of dendritic cells, to produce a solution of differentiated dendritic cells; (c) preparing a pepset of peptides from said protein, wherein said pepset comprises said T-cell epitope; (d) combining said solution of said CD8+ T-cells and anti-CD40 antibody to provide a T-cell and antibody solution, (e) exposing said differentiated dendritic cells and said pepset to said T-cell and antibody solution; and (f) measuring the proliferation of said T-cells in said step (a).