Patent ID: 6927047

Claim:
A method for the manufacture of Mycophenolic acid comprising: loading a contained bioreactor with solid substrate matrix and sterilizing it, mixing the said sterilized solid substrate matrix with Penicillium brevi - compactum, adding 5–20% of glycerol, if desired, incubating the said inoculated solid substrate matrix for 4–7 days at 20–35° C., extracting the fermented solid substrate matrix with an organic solvent, concentrating the organic solvent extract, crystallizing mycophenolic acid by adjusting the pH to 2.0 to 5.0 with an inorganic acid and allowing to stand for 3 to 10 hours, followed by filtration using a filter aid, dissolving the filter aid cake in a water immiscible organic solvent and treating with alumina, filtering the water immiscible organic solvent followed by concentration of the organic layer by distillation, dissolving the concentrate obtained in an alcohol, dispersing the alcoholic solution in water and filtering to get the crude crystals, dissolving the crude crystals thus obtained in an organic solvent, adding another organic solvent to the previous step solution and chilling to 4 to −20° C. to get pure mycophenolic acid crystals.