Patent ID: 7815920

Claim:
A method of preparing an antigen-containing formulation, comprising the steps of: (a) mixing an aqueous solution of antigen with a solution of amino acid in a strong aqueous acid, wherein the amino acid is selected from the group consisting of tyrosine, benzyl-O-octadecanoyl L-tyrosine, and tryptophan; (b) neutralizing the solution of step (a) to co-precipitate the amino acid and antigen; (c) resuspending the co-precipitate of step (b) in buffer; and (d) mixing the solution of step (c) with a TH1-inducing adjuvant, wherein the TH1-inducing adjuvant is selected from the group consisting of monophosphoryl lipid (MPL), 3-de-O-acylated monophosphoryl lipid (3-DMPL), lipopolysaccharide (LPS), 3-de-O-acylated lipopolysaccharide (3-DLPS), diphosphoryl lipid A (DPL), and 3-de-O-acylated diphosphoryl lipid A (3-DDPL).