Patent ID: 8865404

Claim:
A method for determining the nucleic acid sequence of a polynucleotide that is at least 1500 nucleotides in length comprising the steps of: a) providing a polynucleotide; b) cleaving the polynucleotide into a plurality of oligonucleotide sequences; c) immobilizing only one end of each of the plurality of oligonucleotide sequences to a given attachment point on a first end of a substrate; d) arranging the plurality of oligonucleotide sequences substantially linearly on the substrate; e) attaching a label to a non-immobilized end of each of the plurality of oligonucleotide sequences; f) cleaving the plurality of oligonucleotide sequences at a cleavage line substantially perpendicular to said plurality of oligonucleotide sequences to generate a plurality of labelled oligonucleotide fragments; g) collecting the plurality of oligonucleotide fragments; h) sequencing the plurality of oligonucleotide fragments to obtain oligonucleotide reads; i) assembling the oligonucleotide reads; and h) determining the nucleic acid sequence of the assembled oligonucleotide reads to determine the nucleic acid sequence of the polynucleotide.