Patent ID: 8753619

Claim:
A method comprising: maintaining a biotin-containing component comprising a polymer selected from the group consisting of polyethylene glycols and absorbable polymers separately from an avidin-containing component comprising a polymer selected from the group consisting of polyethylene glycols and absorbable polymers; simultaneously applying the biotin-containing component and avidin-containing component directly to tissue; and allowing biotin groups on the biotin-containing component to react with avidin groups on the avidin-containing component to form a three-dimensional crosslinked matrix as a tissue adhesive or sealant, wherein the absorbable polymer of the biotin-containing component or the avidin-containing component is selected from the group consisting of polycaprolactone, poly-D,L-lactic acid, poly-L-lactic acid, poly(lactide-co-glycolide), poly(hydroxybutyrate), poly(hydroxybutyrate-co-valerate), polydioxanone, polyorthoester, polyanhydride, poly(glycolic acid), poly(glycolic acid-cotrimethylene carbonate), polyphosphoester, polyphosphoester urethane, polyglutamic acid, polyaspartic acid, absorbable cyanoacrylates, poly(trimethylene carbonate), copoly(ether-esters), polyalkylene oxalates, polyphosphazenes, polyiminocarbonates, aliphatic polycarbonates, and combinations thereof.