Patent ID: 7087433

Claim:
A method for increasing a CDK9/cyclin-dependent transcription in a cell, the method comprising the steps of: incubating in vitro a human cell comprising human 7SK RNA (SEQ ID NO: 1) and comprising an amount of active CDK9/cyclin, and in which a CDK9/cyclin-dependent transcription is to be increased; introducing in the cell a human 7SK RNA-binding antisense 7SK RNA which increases the amount of active CDK9/cyclin in the cell, and thereby increases said CDK9/cyclin-dependent transcription in the cell, wherein the antisense RNA consists of antisense of nucicotides 221–241 or 95–114 of human 7SK RNA (SEQ ID NO: 1); and detecting a resultant increase in CDK9/cyclin-dependent transcription in the cell, wherein the CDK9/cyclin-dependent transcription is selected from the group consisting of transcription from a recombinant construct, LTR promoter-controlled transcription and HIV transcription.