Patent ID: 7763418

Claim:
A method for detecting an activated Rho GTPase protein comprising: contacting a solid support with a sample comprising an activated Rho GTPase protein, wherein the solid support is linked to an activated Rho GTPase binding peptide, and wherein the activated Rho GTPase in the sample binds the activated Rho GTPase binding peptide; attenuating Rho GTPase protein loss from the solid support by adding an Antigen Presenting Buffer, wherein the Antigen Presenting Buffer comprises tricholoracetic acid; detecting the activated Rho GTPase protein in the sample, wherein the activated Rho GTPase protein remains associated with the solid support during the detection; and wherein prior to detecting the activated Rho GTPase protein in the sample, a Binding Buffer is added when the Rho GTPase binding peptide is ROCK1 or ROCK2.