Patent ID: 8207258

Claim:
A method of using a linear acrylamide copolymer compound for separation of nucleic acid components, said method comprising: providing an aqueous medium comprising a linear acrylamide copolymer comprising a first monomeric component of a formula CH 2 C(R 1 )C(O)NR 1 ′R 1 ″ wherein R 1 is selected from H and methyl moieties; and R 1 ′ and R 1 ″ are independently selected from about H, C 1 to about C 4 linear alkyl moieties, C 1 to about C 4 substituted linear alkyl moieties, C 1 to about C 4 branched alkyl moieties, and C 1 to about C 4 substituted branched alkyl moieties; and a second monomeric component of a formula CH 2 C(R 2 )C(O)NR 2 ′R 2 ″ wherein R 2 is selected from H and methyl moieties; and R 2 ′ and R 2 ″ are independently selected from H, C 1 to about C 24 linear alkyl moieties and C 1 to about C 24 branched alkyl moieties, providing at least one of R 2 ′ and R 2 ″ is at least about C 6 ; introducing said medium to one of a microchannel electrophoresis capillary and a microfluidic sequencing chip; and contacting a mixture comprising at least one of DNA sequence components and RNA sequence components with said medium at an applied voltage and for a time at least partially sufficient for electrophoretic separation of said mixture.