Patent ID: 7579159

Claim:
A kit for conducting a sandwich assay for the determination of human OxLDL (oxidized low density lipoprotein) and human MDA-modified LDL (malondialdehyde-modified low density lipoprotein) in a sample derived from the body fluids or tissues of a human being, said kit comprising: (a) a substrate on which is bound a first antibody for performing the assay, or a first antibody for binding to a substrate for the assay, wherein the first antibody has high affinity for human OxLDL and human MDA-modified LDL, the OxLDL and MDA-modified LDL each having at least 60 substituted lysine moieties per apo (apolipoprotein) B-100 moiety, and (b) a labeled antibody having a high affinity for human OxLDL and human MDA-modified LDL that become bound to the first antibody during the assay, wherein the first antibody and the antibody that is labeled are each independently selected from the group consisting of (i) monoclonal antibody mAb-4E6 produced by hybridoma Hyb4E6, which was deposited at the BCCM (Belgian Coordinated Collections of Microorganisms) under deposit accession number LMBP 1660 CB on Apr. 24, 1997, and (ii) monoclonal antibody mAb-8A2 produced by hybridoma Hyb8A2, which was deposited at the BCCM under deposit accession number LMBP 1661 CB on Apr. 24, 1997, provided that at least the first antibody or antibody that is labeled is monoclonal antibody mAb-4E6.