Patent ID: 8871473

Claim:
A method for producing γ-CD (cyclodextrin) with isoamylase and γ-CGTase (A223K) (cyclodextrin glycosyltransferase) simultaneously, comprising the steps of: a) suspending starch in water to a final concentration of 5-20% and heating to 60-90° C. for about 5-15 min under constant stirring to make a starch slurry; b) cooling down the starch slurry to 40-60° C. and adjusting the pH to 7.0-8.0; c) adding simultaneously 10-100 U γ-CGTase (A223K) and 10-100 U isoamylase per gram of starch to the starch slurry to make a reaction mixture; d) adding 5% (w/v) organic complexing agent to the reaction mixture and incubating at 40-60° C. for 6-12 hours; and e) removing the organic complexing agent and obtaining pure γ-CD by crystallization.