Patent ID: 8338118

Claim:
A process for detecting one of a presence of an analyte, an amount of the analyte, and an activity of the analyte in a sample by fluorescence measurement, comprising the following steps: (a) providing a detection medium comprising: (i) at least part of the sample, including a body fluid; (ii) one of a fluorophore which has an excitation range with at least one excitation maximum at a first wavelength, and a fluorophore precursor from which the fluorophore can be produced in the presence of the sample; and (iii) an absorber which absorbs light over a part of the excitation range of the fluorophore, resulting in an altered effective excitation range of a system consisting of the fluorophore and the absorber with an excitation maximum at a second wavelength which differs from the first wavelength; (b) contacting the one of the fluorophore and the fluorophore precursor, the absorber, and the at least part of the sample; (c) illuminating the detection medium with light in order to excite the fluorophore in a region of the second wavelength; and (d) determining a fluorescence emission of the fluorophore at one or more measuring wavelengths, the emission being indicative of at least one of the presence, the amount and then activity of the analyte in the sample.