Patent ID: 7235397

Claim:
A serum-free media for culturing spirochetes comprising 1.25 g/L glucose, 1 g/L sucrose, 1.3 g/L fructose, 1.25 g/L lactose, 1.2 g/L mannose, 9 g/L sodium chloride, 1.8 g/L potassium chloride, 0.5 g/L lithium chloride, 0.185 g/L calcium chloride-2H 2 0, 0.1 g/L anhydrous magnesium sulfate, 0.06 g/L monobasic anhydrous potassium phosphate, 0.05 g/L dibasic anhydrous sodium phosphate, 2.22 g/L peptone, 1.998 g/L neopeptone, 0.052 g/L manganese, 0.044 g/L magnesium, 0.5 ml/L SITE+3 liquid media supplement, 0.25 ml/L N 1 medium supplement, 1 ml/L 50×RPMI 1640, 10 g/L MEM Essential Amino Acids, 10 ml/L MEM Non-Essential Amino Acids, 2 ml/L pluronic F-68 10% solution, 0.53 g/L Vanderzandt's supplement, 5 ml/L L-methionine, 22.64 g/L sodium pyruvate, 4.6 g/L L-glutamine, 0.025 g/L brain extract type 1, 0.3 g/L brain extract type 6, 0.053 g/L sphingomyelin, 0.1 ml/L 1000× concentrated lipid solution, 5 g/L N-acetyl glucosamine, 0.12 g/L thyroxine, 0.22 g/L thyroid powder, 0.008 g/L estradiol, 1.6 g/L glycine, 0.05 g/L ferrous sulfate, 0.51 g/L muscle extract powder, 0.001 g/L myelin basic protein, 10 g/L lactalbumin hydrolysate, 0.53 g/L granulated yeast, 0.05 g/L sodium bicarbonate, 0.66 g/L brain heart infusion, and 5.25 g/L Hepes.