Patent ID: 7455989

Claim:
A method of detecting a compartment of a cell or a macromolecule of the cell, the method comprising: (a) expressing a polynucleotide encoding a chimeric polypeptide in the cell, said chimeric polypeptide including: (i) a first polypeptide region being capable of specifically binding at least one detectable molecule, said first polypeptide region comprising a single chain Fv; and (ii) a second polypeptide region being capable of specifically binding the macromolecule of the cell; or targeting into a specific cell compartment, said second polypeptide region comprising S-AKAP84; (b) exposing the cell to said detectable molecule under conditions suitable for binding of said detectable molecule to said first polypeptide region; and (c) analyzing a presence of said detectable molecule in the compartment of the cell, or in association with said macromolecule of the cell, thereby detecting the compartment of the cell or macromolecule of the cell.