Patent ID: 7217557

Claim:
A method for crystallizing the chalcone synthase of SEQ ID NO:1 or mutant thereof, wherein said mutant chalcone synthase has one or more mutations selected from the group consisting of Xaa 164 S, Xaa 164 D, H 303 A, H 303 Q, H 303 D, H 303 T, N 336 A, N 336 D, N 336 Q, N 336 H, F 215 S, F 215 Y, and F 215 W, wherein said method comprises growing crystals of said chalcone synthase or mutant thereof by vapor diffusion at 4° C. in 2 μL drops, wherein said drops contain a 1:1 mixture of 25 mg/mL of said chalcone synthase and crystallization buffer, and wherein said crystallization buffer comprises 2.2–2.4 M ammonium sulfate and 0.1 M PIPES, pH 6.5.