Patent ID: 7432101

Claim:
A method for replicating a native or recombinant vaccinia virus comprising the steps of: 1) producing avian embryonic derived stem cells by: a) culturing avian embryonic cells in a complete culture medium complemented in serum containing: i) exogenous growth factors comprising the trophic factors SCF, IGF-1 and bFGF, and cytokines whose action is through a receptor which is associated with the gp130 protein, said cytokines being selected from the group consisting of LIF, interleukin 11, interleukin 6, interleukin 6 receptor, CNFT, oncostatin and cardiotrophin; and ii) a feeder layer; b) passage by modifying the culture medium so as to obtain the withdrawal of said growth factors, of the serum and/or of the feeder layer; c) establishing adherent or non-adherent cell lines capable of proliferating in a basal medium in the absence of exogenous growth factors, serum and/or inactivated feeder layer; 2) inoculating the resultant avian embryonic derived stem cells with viral particles of said vaccinia virus; and 3) culturing said cells in a basal medium until cell lysis occurs and newly produced viral particles of said vaccinia virus are released in said medium.