Patent ID: 8084248

Claim:
A reverse genetic composition for a Phlebovirus , comprising: at least one anti-viral sense RNA expression plasmid that encodes a non-viral gene inserted in place of a non-structural protein (NSs) open reading frame (ORF) in the Phlebovirus Small (S) segment, such that said non-structural protein (NSs) open reading frame (ORF) for said Phlebovirus is deleted but the N gene is not deleted; at least one anti-viral sense RNA expression plasmid that encodes a Phlebovirus Large (L) segment; at least one anti-viral sense RNA expression plasmid that encodes a Phlebovirus Medium (M) segment; at least one viral protein expression plasmid that expresses a Phlebovirus L protein; at least one viral protein expression plasmid that expresses a Phlebovirus N protein; and at least one viral protein expression plasmid that expresses Phlebovirus envelope proteins.