Patent ID: 7361508

Claim:
A method for targeting the insertion of a polynucleotide of interest to a specific chromosomal site in a genome of a plant cell, said method comprising: a) introducing into the plant cell a transfer cassette, said transfer cassette comprising said polynucleotide of interest flanked by a first and a second recombination site, wherein said first and said second recombination sites are non-identical; and, wherein said genome of said plant cell comprises a target site comprising the first and the second non-identical recombination sites of said transfer cassette, wherein said first and said second non-identical recombination sites can recombine with their identical recombination site; and, b) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites and thereby allow for the directional targeting of a nucleotide sequence of interest at the target site.