Patent ID: 7012093

Claim:
A process for extraction of a novel compound and multiple fluorescent dye from Holothuria scabra seacucumber, said process comprising the steps of: a. collecting the animals from the shores during a low tide, and maintaining in glass tanks containing seawater in the laboratory for taxonomic identification and further use; b. washing animals thoroughly with Milli-Q water and anaesthetized by chloroform/menthol in a closed jar; c. removing the viscera and the skin portion of the animals by peeling off by scraping with a scalpel for lyophilizing; d. taking lyophilized skin of step (c) with required quantity of ultra pure water and keeping on a shaker for 4 hours, followed by filtration through Whattmann paper 1 and sintered glass filter; e. evaporating the filtrate of step (d) and precipitating the compound using organic solvents; f. dissolving the precipitate in minimum volume of water and re-precipitating with an organic solvent to remove the salts; g. centrifuging to collect the residue and discarding the filtrate; h. drying the residue of step (g) to obtain a dry powder; i. dissolving the dry powder of step (h) in water, loading to ion-exchange column j. eluting the column of step (i) with water; k. lyophilizing the water eluted fractions of step (j) l. loading the lyophilized material of step (k) on to DEAE cellulose column, eluting with phosphate buffer, m. desalting repeatedly the phosphate buffer eluted fraction of step (l) using PD10 column and n. obtaining the required bioactive compound of formula C 22 H 48 O 66 N 2 S 10 Si 7 represented by one of the following structural formulae: