Patent ID: 8013147

Claim:
A nucleotide analog of the formula in which R 1 is hydrogen, halogen, hydroxy, thio, carboxy, lower alkyl, lower alkenyl, lower alkinyl, aryl, lower alkyloxy, aryloxy, aralkyl, aralkyloxy or a reporter group, R 3 and R 4 each is independently hydrogen, hydroxy, thio, amino, lower alkyloxy, lower alkenoxy, lower alkinoxy, an ester, amide or a reporter group, R 5 is hydrogen, hydroxy, thio, amino, a trivalent phosphorus group, a pentavalent phosphorus group, an ester, amide or a reporter group, or R 4 and R 5 together form a further bond between C-2′ and C-3′ or an acetal group, R 6 is hydrogen, hydroxy, thio, or amino, R 7 is hydrogen, a monophosphate, a diphosphate a triphosphate group, an alpha, beta or gamma thiophosphate analogue of a phosphate group, an ester or an amide group, X is methine substituted with halogen, hydroxy, thio, amino, carboxy, lower alkyl, lower alkenyl, lower alkinyl, aryl, lower alkyloxy, aryloxy, aralkyl, aralkyloxy or a reporter group, each reporter group is independently selected from the group consisting of a hapten and a fluorophore, optionally linked via a linker group; and salts thereof.