Patent ID: 8143376

Claim:
An engineered T-cell receptor (TCR) comprising (1) a 1G4 TCR α chain variable domain shown in SEQ ID NO:1; and (2) a 1G4 TCR β chain variable domain shown in SEQ ID NO:2, wherein said TCR comprises: (a) one or more amino acid substitutions relative to the native 1G4 TCR α chain variable domain shown in SEQ ID NO:1 selected from the group consisting of 20A, 51P, 51S, 51T, 51M, 52P, 52F, 52G, 53W, 53H, 53T, 94H, 94A, 95L, 95M, 95A, 95Q, 95Y, 95E, 95I, 95F, 95V, 95N, 95G, 95S, 95R, 95D, 96L, 96T, 96Y, 96I, 96Q, 96V, 96E, 96X, 96A, 96W, 96R, 96G, 96H, 96K, 96D, 97D, 97N, 97V, 97S, 97A, 97T, 98P, 98H, 98S, 98T, 98W, 98A, 99T, 99Y, 99D, 99H, 99V, 99N, 99E, 99G, 99Q, 99K, 99A, 99I, 99R, 100F, 100M, 100D, 101P, 101T, 101M, and 103A using the numbering shown in SEQ ID NO:1; and/or (b) one or more amino acid substitutions relative to the native 1G4 TCR β chain variable domain shown in SEQ ID NO:2 selected from the group consisting of 18V, 50S, 50A, 51V, 51I, 52Q, 53T, 53M, 55R, 56R, 70I, 94N or 94F, 95L, 97G or 97D using the numbering shown in SEQ ID NO:2.