Patent ID: 7981645

Claim:
A process for the stereoselective decarboxylation of malonic acid derivatives of the formula (I) the process comprising: using a decarboxylase to produce a compound of the formula (II) wherein X is selected from the group consisting of OH, SH, C 2 -C 10 alkyl, C 1 -C 10 alkoxy, and NH 2 , and R is selected from the group consisting of aryl, heteroaryl, condensed aryl, and condensed heteroaryl radical, wherein R is substituted or unsubstituted, and optionally wherein a substituent is selected from the group consisting of a halogen, alkyl, alkenyl, and alkynyl radical; wherein said decarboxylase comprises the amino acid sequence of SEQ ID NO: 1, except for a mutation at an amino acid position of SEQ ID NO: 1 selected from the group consisting of 15, 17, 19, 22, 24, 25, 32, 41, 42, 46, 47, 53, 60, 61, 63, 68, 74, 75, 83, 84, 85, 87, 94, 103, 105, 112, 116, 119, 121, 128, 139, 142, 155, 163, 165, 168, 173, 178, 199, 201, 202, 203, 204, 205, 210, 221, 222, 224, 225, 226, 227, 228, 229, 230, 235, 238, 239, 240, and combinations thereof; wherein said mutation comprises replacing the amino acid at said position with any other amino acid, wherein, at position 188, the cysteine residue is not replaced, and wherein not more than 10 amino acids are inserted into and/or deleted from the sequence according to SEQ ID NO: 1.