Patent ID: 8236526

Claim:
An isolated polynucleotide comprising at least one of items (a) and (b): (a) a polynucleotide comprising the nucleotide sequence selected from the group consisting of an nucleotide sequence set forth in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12; (b) a polynucleotide encoding a protein comprising the amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11, and having the ability to bond with a peroxide of coelenterazine or a peroxide of a coelenterazine derivative so as to form a holoprotein that emits light under the action of a calcium ion.