Patent ID: 6933106

Claim:
An immunobead platelet assay using flow cytometry comprising the steps of: preparing IgG, IgM and IgA coated bead based Ig control reagent by adding a bead coated with IgG or subclasses thereof to an IgG test set comprising a test tube labeled IgG and an IgG control tube, adding a bead coated with IgM or subclasses thereof to an IgM test set comprising a test tube labeled IgM and an IgM control tube, adding a bead coated with IgA or subclasses thereof to an IgA test set comprising a test tube labeled IgA and an IgA control tube, wherein an assay has a least two test sets; adding patient platelets to each test tube labeled IgG, IgM and IgA respectively and adding Type O control platelets to each of the IgG, IgM and IgA control tubes; adding fluorochrome conjugated species specific Ig to each of the IgG, IgM and IgA control tubes, and fluorochrome conjugated species specific anti-human IgG, IgM and IgA to each of the test tubes labeled IgG, IgM and IgA respectively; incubating the tubes for 15 minutes in the dark; washing once with isotonic saline and adding additional isotonic saline to a final volume, and reading each tube on a flow cytometer to analyze the contents thereof.