Patent ID: 8145437

Claim:
A method for determining and producing a binding amino acid sequence (target oligopeptide) having binding affinity for a known amino acid sequence (query protein), the method comprising: a) determining a query nucleotide sequence which encodes the query oligopeptide to provide a series of codons, wherein each codon has a 1 st , 2 nd , and 3 rd nucleotide and the nucleotide sequence has a 5′ and 3′ end; b) creating a nucleotide sequence which is complementary to the query nucleotide sequence, except that the 2 nd nucleotide in each codon is an undefined nucleotide; c) determining the reverse sequence of the created nucleotide sequence; d) preparing a pool of target nucleotide sequences wherein the undefined 2 nd nucleotide of each codon comprises equal amounts of A, T/U,G,C nucleotides; e) cloning the target nucleotide sequence pool; f) preparing a protein expression library from the target nucleotide sequence pool; g) contacting the prepared expression library with the query protein; and h) identifying binding complexes between the query protein and one or more target oligopeptides.