Patent ID: 8293242

Claim:
A method of extracting alpha-1-antitrypsin from plasma, comprising: providing a non-salted, non-precipitated, previously frozen blood product; salting the previously frozen blood product to produce a first intermediate having a total salt content of between 11-13% wt; centrifuging the first intermediate to produce a first supernatant and a first paste; salting the first supernatant to produce a second intermediate with a total salt content of between at least 21-23% wt; centrifuging the second intermediate to produce a second supernatant and a second paste; rehydrating the first paste, de-salting the first paste and the second supernatant, and passing the de-salted first paste and second supernatant through at least one of an ion exchange column or an affinity column to produce a first and second alpha-1-antitrypsin effluent; and combining the first and second effluents to produce a product containing the alpha-1-antitrypsin.