Patent ID: 7977051

Claim:
A method for obtaining a gene encoding an EGIII like cellulase comprising the steps of (i) preparing genomic DNA from an organism of interest; (ii) preparing a DNA primer encoding an amino acid string selected from the group consisting of one or more of the following: (a) Asn-Asn-(Xaa)-Trp-Gly (SEQ ID NO: 1), wherein (Xaa) at position 3 is Leu, Phe, Lys or Ile; (b) Glu-(Xaa)-Met-Ile-Trp (SEQ ID NO: 2), wherein (Xaa) at position 2 is Phe or Ile; (c) Gly-Thr-Glu-Pro-Phe-Thr (SEQ ID NO: 3); (d) (Xaa)-(Xaa)-(Xaa)-(Xaa)-(Xaa) (SEQ ID NO: 42), wherein (Xaa) at position 1 is Ser, Tyr, Cys, Trp, Thr, Asn, Lys, or Arg, (Xaa) at position 2 is Val or Pro, (Xaa) at position 3 is Lys or Ala, (Xaa) at position 4 is Ser or Ala, and (Xaa) at position 5 is Tyr or Phe; and (e) Lys-Asn-Phe-Phe-Asn-Tyr (SEQ ID NO: 5); (iii) mixing said genomic DNA from step (i) and said DNA primer from step (ii) under conditions suitable for the identification of all or part of a gene fragment in said genomic DNA corresponding to said DNA primer; and (iv) isolating said all or part of said gene corresponding to said fragment from said genomic DNA.