Patent ID: 8163294

Claim:
An immunogenic composition comprising: a growth regulatable recombinant Bacille Calmette Guérin (rBCG) expressing at least one Mycobacteria major extracellular protein selected from the group consisting of 12 kDa protein, 14 kDa protein, 16 kDa protein, 23.5 kDa protein, 24 kDa protein, 30 kDa protein, 32A kDa protein, 32B kDa protein, 45 kDa protein, 58 kDa protein, 71 kDa protein, 80 kDa protein, and 110 KD protein, and combinations thereof, wherein at least one of said at least one Mycobacteria major extracellular proteins are expressed on one or more extrachromosomal nucleic acid sequences, wherein said growth regulatable rBCG is selected from the group consisting of an auxotroph wherein pantothenic acid is used to regulate the growth of said auxotroph, and a siderophore mutant wherein the siderophore is mycobactin or exochelin, and wherein said Mycobacteria major extracellular proteins are over-expressed and secreted.