Patent ID: 8506971

Claim:
A method of making a preparation comprising the lipopolysaccharide (LPS) of a Gram-negative bacterium, the method comprising: (i) mixing the LPS or the LPS formulated in liposomes (LPS liposomes) with the lipidated human-transferrin receptor subunit B protein (TbpB) of Neisseria meningitidis or a lipidated fragment thereof that comprises one or more T-helper epitopes; (ii) formulating the LPS and the lipidated TbpB of N. meningitidis or the lipidated fragment thereof together in liposomes; or (iii) conjugating the LPS with the lipidated TbpB of N. meningitidis or the lipidated fragment thereof; wherein the mixing, the formulating, or the conjugating yields a preparation which does not contain outer membrane vesicles (OMVs).