Patent ID: 8741619

Claim:
A method to purify a solution containing a target protein by utilizing an acetate inorganic layered material, comprising steps of: providing an inorganic layered material; adding an organic solvent into the inorganic layered material to swell the inorganic layered material; mixing an acetic acid solution with the swelled inorganic layered material to form an acetified mixture containing an acetate inorganic layered material, wherein the acetic acid solution is non-diluted glacial acetic acid; performing a cleaning process by using a cleaning solvent to clean the acetified mixture; performing at least one centrifuge separating process with the speed between 6,000 rpm and 14,000 rpm to separate the cleaned acetified mixture into a first solid-liquid two phases solution and to collect a plurality of solid phases containing the acetate inorganic layered material from the first solid-liquid phases solution; drying the plurality of solid phases to obtain the acetate inorganic layered material; providing the solution containing the target protein; adding the acetate inorganic layered material into the solution containing the target protein to form a first mixture; performing a first separating process to separate the first mixture into a second solid-liquid two phases solution; resuspending a solid phase of the second solid-liquid two phases solution by adding an phosphate buffer solution into the solid phase of the second solid-liquid two phases solution to form a second mixture; performing a second separating process to separate the second mixture into a third solid-liquid two phases solution; and extracting a liquid phase from the third solid-liquid two phases solution to obtain a purified target protein.