Patent ID: 7015033

Claim:
An expression vector for expression of a recombinant protein in a host cell, comprising: a nucleic acid molecule encoding a non-proteolytic mutant of a Haemophilus Hin47 protein wherein said Hin47 protein is mutated at amino acid 91, 121 or 197 and said nucleic acid molecule includes a portion encoding the leader sequence for said non-proteolytic mutant, at least one additional nucleic acid molecule encoding the recombinant protein, wherein the recombinant protein is a Hia protein of a strain of Haemophilus influenzae which is N-terminally truncated, and at least one regulatory element operatively connected to said first nucleic acid molecule and at least one regulatory element operatively connected to said at least one additional nucleic acid molecule to effect expression of at least said recombinant protein in the host cell.