Patent ID: 7708950

Claim:
A microfluidic device comprising: a processing layer that applies a predetermined process to a plurality of subject fluids, the processing layer comprising a reaction portion that makes at least two of the plurality of subject fluids react with each other; a plurality of temperature control layers, two or more of the temperature control layers being in fluid communication with each other and being configured to maintain a predetermined temperature environment in the processing layer by circulating a fluid at a predetermined temperature, and one or more of the temperature control layers comprising a closed space that shields heat conduction from the processing layer and is a vacuum; and a washing layer comprising a washing channel in fluid communication with at least one washing fluid channel and a flow-dividing portion, the washing fluid channel being configured to provide washing fluid to the washing channel, the washing channel being configured to have a reaction fluid flow in a center portion of the washing channel sandwiched between layers of the washing fluid, and the flow-dividing portion being configured to separate the reaction fluid from the washing fluid; wherein the reaction portion of the processing layer is adjacent to and sandwiched between at least two of the plurality of temperature control layers.