Patent ID: 7560268

Claim:
A process for manufacturing a biochip, the process comprising steps of: (a) placing a probe aqueous solution on a substrate; wherein the probe aqueous solution includes a compound represented by Formula (1) or (2) below or a hydrolysate thereof and a probe: CF 3 (CF 2 ) 8 C 2 H 4 SiR 3-x (OY) x (1) CF 3 (CF 2 ) 8 C 2 H 4 Si[(O—CH 2 CH 2 ) m —OR] 3 (2) where, in the Formula (1), R and Y denote a methyl group, an ethyl group, a propyl group or a butyl group independently, n is a natural number in a range of 1 to 10, x is a natural number in a range of 1 to 3, and when x=1, two Rs exist, the Rs being the same or different from each other, and in the Formula (2), R denotes a methyl group, an ethyl group, a propyl group or a butyl group, n is a natural number in a range of 1 to 10, and m is a natural number in a range of 1 to 10, wherein the probe has at least one functional group selected from the group consisting of —NH 2 , —COOH, —SO 3 H and —SH, and wherein the substrate has at least one functional group selected from the group consisting of—OH, —NH 2 , —COOH, —SO 3 H and —CN on the surface of the substrate, (b) fixing the compound represented by Formula (1) or (2) or a hydrolysate of the compound represented by Formula (1) or (2) through a chemical reaction between a —SiR 3-x (OY) x group or a —Si[(O—CH 2 CH 2 ) m —OR] 3 group of the compound represented by Formula (1) or (2), or —SiOH of each hydrolysate of the compound represented by Formula (1) or (2), to the functional group of the substrate; (c) fixing the probe onto the substrate by chemical bonding or physical adsorption of the functional group of the substrate and the functional group of the probe; wherein step (b) and step (c) are carried out simultaneously, and wherein the probe aqueous solution shrinks by repulsion on the substrate due to a functional group CF 3 (CF 2 ) n — included in a compound represented by Formula (1) or (2) or a hydrolysate thereof, which has been fixed in step (b); (d) evaporating the probe aqueous solution; and (e) washing the substrate.