Patent ID: 8007746

Claim:
A microfluidic device comprising: a plurality of reaction cells in a first layer of said device, wherein each reaction cell comprises (i) a sample chamber and a reagent chamber, wherein said chambers are connected to each other through an interface channel; and (ii) an interface valve in said interface channel between the sample chamber and the reagent chamber that, when actuated, fluidically isolates said sample chamber and said reagent chamber from each other; a plurality of first reagent fluid channels in said first layer, wherein each first reagent fluid channel (i) terminates in a reagent chamber and (ii) is in fluid communication with a via that connects the first reagent channel and a second reagent fluid channel located in a different layer of the device, wherein each second reagent fluid channel is in fluid communication with a plurality of reagent chambers; a plurality of sample fluid channels in said first layer, wherein each sample fluid channel is in fluidic communication with a plurality of sample chambers, and wherein each reaction cell is in fluidic communication with a different first reagent fluid channel.