Patent ID: 8802111

Claim:
A process for preparing a combination vaccine, wherein the vaccine comprises: (i) a non-ionic surfactant, (ii) a hepatitis B virus (HBV) surface antigen (HBsAg), and (iii) an antigen from at least one non-HBV pathogen, and wherein the process comprises: (a) purifying the HBV surface antigen from recombinant yeast cells, wherein the purification includes a step in which the yeast cells are disrupted in the presence of the non-ionic surfactant, to give a purified HBsAg component wherein the process does not involve a step of adding the non-ionic surfactant as a separate component after HBsAg purification; (b) adsorbing the purified HBsAg component to aluminium phosphate; and (c) combining the adsorbed HBsAg component with at least one further antigen from a non-HBV pathogen, to give the combination vaccine.