Patent ID: 8916530

Claim:
A method for individualized cancer therapy comprising: obtaining a specimen of cancer tissue and normal tissue from a patient having at least one cancer recurrence; extracting total protein and RNA from the cancer tissue and normal tissue; obtaining a protein expression profile of the cancer tissue and normal tissue; identifying over-expressed proteins in the cancer tissue; comparing the protein expression profile to a gene expression profile; identifying at least one prioritized protein target from the gene expression profile; designing a RNA interference expression cassette to modulate the expression of at least one gene encoding the prioritized protein target wherein the RNA interference expression cassette is: a single contiguous bifunctional RNA molecule that comprises: a first double stranded sequence comprising a guide strand sequence that is fully complementary to a passenger strand and to an mRNA transcript encoded by the target gene; and a second double stranded sequence that is partially complementary to a mismatched passenger strand and complementary to the mRNA transcript encoded by the target gene, wherein the bifunctional RNA molecule activates cleavage-dependent and cleavage-independent RNA-induced silencing complex for reducing the expression level of the target gene; incorporating the RNA interference expression cassette into a delivery vehicle; and providing the patient with an effective amount of the delivery vehicle to treat the cancer.