Patent ID: 7985563

Claim:
A method of identifying a compound that decreases proline hydroxylation of the alpha subunit of hypoxia inducible factor (HIFα), the method comprising: a) contacting a compound and a non-hydroxylated HIFα polypeptide comprising one or more hydroxylatable proline residues under conditions wherein the HIFα polypeptide, in the absence of compound, is hydroxylated on at least one proline residue; b) measuring the degree of prolyl hydroxylation of HIFα in the presence of compound; and c) comparing the degree of prolyl hydroxylation of HIFα in the presence of compound to the degree of prolyl hydroxylation of HIFα in the absence of compound, wherein a decrease in the degree of prolyl hydroxylation in the presence of compound compared to the degree of prolyl hydroxylation in the absence of compound identifies a compound that decreases proline hydroxylation of HIFα.