Patent ID: 8431347

Claim:
A method for isothermal DNA amplification, the method comprising: providing to the DNA to be amplified an amplification mix comprising: a first primer being at least partially complementary to a region of DNA having a CpG dinucleotide, the first primer containing at least one deoxyinosine and does not include a ribonucleotide, a second primer being at least partially complementary to a region of DNA having a CpG dinucleotide, the first primer containing at least one deoxyinosine and does not include a ribonucleotide, an exonuclease deficient DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a deoxyinosine in double-stranded DNA and causes a nick in one DNA strand at or near the deoxyinosine; and amplifying the DNA substantially without thermal cycling, wherein inosine substitutes the position of guanine in a CpG dinucleotide of the DNA.