Patent ID: 7459317

Claim:
A method of detecting the presence of an analyte in a sample, said method comprising: (a) mixing said sample with a chemiluminescent enzyme-labeled binding member and a second binding member immobilized on a surface of a solid support to produce a first composition, wherein analyte present in said sample binds to both said enzyme-labeled binding member and said second binding member to produce solid support surface immobilized binding member complexes comprising said analyte, said enzyme-labeled binding member and said second binding member; (b) separating unbound enzyme-labeled binding member from said solid support surface to produce a second composition; (c) adding an oxidant to the second composition; (d) adding a light wavelength converter to the second composition; and (e) detecting binding complexes immobilized on said solid support surface to detect the presence of said analyte in said sample, wherein said detecting comprises detecting second light emitted by said light wavelength converter, said wavelength converter generating said second light in response to absorbing first light generated by said enzyme-labeled binding member, said first light being generated in response to reaction of said enzyme-labeled binding member with the oxidant.