Patent ID: 7727474

Claim:
A pipetting arrangement comprised of a pipette, a closure and a container, the pipetting arrangement providing for withdrawal of liquids from a reagent container ( 7 ), wherein the pipetting arrangement comprises a pipette ( 1 ) having an actuator sleeve ( 2 ) positioned around and permanently attached to the pipette ( 1 ), the actuator sleeve ( 2 ) being axially spaced from an insertion end ( 5 ) of the pipette ( 1 ) and wherein a closure for the reagent container ( 7 ) is accessible by said pipette ( 1 ) and has an insertion end ( 5 ) consisting of a cap part ( 6 ) for attachment to the reagent container ( 7 ) and a conical insert ( 4 ), wherein the conical insert ( 4 ) is incorporated into an opening of the cap part ( 6 ) and has a conical or pyramidal wall which terminates in a entrance point ( 10 ) in the direction of the reagent container ( 7 ) in that the conical or pyramidal wall of the conical insert ( 4 ) is divided into flaps ( 11 ) by at least one cut ( 12 ) and has an actuation ridge ( 3 ) on a side facing away from the reagent container ( 7 ), the actuation ridge ( 3 ) having a diameter defining an opening larger than the diameter of the pipette ( 1 ) and smaller than the diameter of the actuator ( 2 ) on the pipette ( 1 ), wherein the pipette ( 1 ) need not engage the conical insert ( 4 ) to enter the reagent container ( 7 ) because the actuator ( 2 ) engages the flaps ( 11 ) to deflect the flaps away from the pipette ( 1 ), and wherein the flaps ( 11 ) close upon withdrawal of the actuator ( 2 ) from the ridge ( 3 ) after the pipette ( 1 ) leaves the entrance point ( 10 ) of the conical insert ( 4 ), wherein when the pipette ( 1 ) is withdrawn together with the actuation sleeve ( 2 ), walls of the conical insert ( 4 ) reclose since the actuating sleeve ( 2 ) no longer forces the actuation ridge ( 3 ) apart again tightly sealing the reaction container ( 7 ), which prevents contamination of contents in the reaction container ( 7 ) during withdrawal of the pipette ( 1 ).