Patent ID: 7531726

Claim:
A system for nucleic acid sequencing comprising: a) a scanning probe microscope; b) a substrate; c) at least one target molecule immobilized on the substrate, wherein the target molecule is a nucleic acid; d) one or more coded oligonucleotide probes ligated to the nucleic acid, wherein each of the coded oligonucleotide probes is attached to at least one nanobarcode; and e) a surface, wherein the coded oligonucleotide probes are attached to the surface after the coded oligonucleotide probes are separated from the nucleic acid; and f) a free flow electrophoresis device, wherein the free flow electrophoresis device is configured to align the separated coded oligonucleotide probes on the surface wherein the surface comprises alternating bands of conductive and non-conductive materials such that in the presence of an alternating current electrical field, the free flow electrophoresis device is configured to align the separated coded oligonucleotide probes on the surface.