Patent ID: 8628944

Claim:
A method for producing ethanol from xylose using recombinant Saccharomyces cerevisiae , the method comprising: transforming the recombinant Saccharomyces cerevisiae to express NADH-preferring xylose reductase (XR) to convert xylose into xylitol using NADH as a cofactor; transforming the recombinant Saccharomyces cerevisiae to express xylitol dehydrogenase (XDH) to convert xylitol into xylulose using NAD + as a cofactor; transforming the recombinant Saccharomyces cerevisiae to over-express xylulokinase (XK) to convert xylulose into xylulose 5-phosphate; and transforming the recombinant Saccharomyces cerevisiae to over-express transaldolase 1 (TAL1) to convert sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate into erythrose 4-phosphate and fructose 6-phosphate, wherein the recombinant Saccharomyces cerevisiae is transformed to entirely or partially fragmentize acetaldehyde dehydrogenase encoding gene ALD6 to disrupt expression on acetaldehyde dehydrogenase that converts acetaldehyde into acetic acid.