Patent ID: 7906341

Claim:
A compound, represented by formula II; including a salt form and/or hydrate form thereof; wherein, W is an atom or group that is substituted for at least one M group of the six membered heterocyclic ring and is located ortho, meta or para to the nitrogen of the six membered ring and is —N(H)—, —N(R″)—, —N(R′″)—, —P(R″)—, —P(R′″)—, —O— or —S—; each remaining group M is, independently of the other, —CM' 2 —, wherein each M′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 8 , —OR 8 , —SR 8 , —R 8 ′OR 8 or —R 8 ′SR 8 ; J is a group represented by formula —CJ′ 2 —, wherein each J′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 3 , —OR 3 , —SR 3 , —R 3 ′OR 3 or —R 3 ′SR 3 ; K is a group represented by formula —(CK′ 2 ) n — or —((CK′ 2 ) m —X 2 —(CK′ 2 ) m ) p —, wherein n is an integer from 2 to 10, each m is, independently of the other, an integer from 1 to 5, p is an integer from 1 to 4 and each K′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 4 , —OR 4 , —SR 4 , —R 4 ′OR 4 or —R 4 ′SR 4 ; either 1) R 1 is hydrogen, deuterium or R 6 and R 2 is hydrogen, deuterium or R 7 ; or 2) R 1 and R 2 taken together is a group represented by formula —(CR′ 2 ) q — or —((CR′ 2 ) m —X 2 —(CR′ 2 ) m ) p — that forms a ring that bridges the two nitrogen atoms, wherein q is an integer from 1 to 10, each m is, independently of the other, an integer from 1 to 5, p is an integer from 1 to 4 and each R′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 5 , —OR 5 , —SR 5 , —R 5 ′OR 5 or —R 5 ′SR 5 ; X 1 is ═O, ═S, ═NH or ═NR 7 ; each X 2 is, independently of the other, —O— or —S—; each R″ is, independently of the other, alkyl, alkenyl, alkynyl, aryl, heteroaryl or arylalkyl; each R′″ is H 2 N—R 9 ′—, H(R 10 )N—R 9 ′—, (R 10 ) 2 N—R 9 ′—, HO—R 9 ′—, HS—R 9 ′— or a cleavable linker that cleavably links the compound to a support; Z is hydrogen or a covalently linked analyte; wherein, each R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and/or R 10 is, independently of the other, alkyl, alkenyl, alkynyl, aryl, heteroaryl or arylalkyl; each R 3 ′, R 4 ′, R 5 ′, R 6 ′, R 8 ′ and/or R 9 ′ is, independently of the other, alkylene, alkenylene, alkynylene, arylene or alkylarylene; and the compound comprises at least one isotopically enriched site.