Patent ID: 7550288

Claim:
A method of performing a reaction using a biochemical analysis unit having a flow-through area in which plural spot areas are arranged comprising: setting the biochemical analysis unit into a chamber; supplying the chamber with a solution containing a target as a test substance, wherein the solution flows through a flow-through area; performing specific binding of a probe and the target in plural spot areas when the solution flows through the flow-through area; and wherein a pressure loss of the solution is controlled from 1 kPa to 1 MPa to allow the solution to flow equivalently through the flow-through area, and the pressure loss is controlled from 1 kPa to 1 MPa by the following conditions: a flow speed from 0.1 mm/s to 20 mm/s of the solution containing a target flowing equivalently through the flow-through area, and a temperature from 25° C. to 80° C. of the solution containing a target when the solution is supplied into the chamber.