Patent ID: 8841088

Claim:
A method for the production of a desired chemical compound in a microorganism, wherein the stability of an mRNA transcript from a gene involved in the biosynthesis of said compound is increased by the introduction of a DNA sequence compared to the stability of said mRNA without said introduced DNA sequence wherein the DNA sequence is introduced seven or more nucleotides downstream of the transcription start of said gene and wherein said DNA sequence is a chromosomal nucleotide DNA sequence of the cggR-gapA intercistronic region of Bacillus subtilis and consists of at least 15 nucleotides forming one or more stem loop secondary structures showing (i) a double-stranded stem structure of at least 6 base pairs and a hairpin loop structure of 3-30 nucleotides and (ii) a calculated thermodynamic stability (ΔG) of said structure of −2.8 kcal/mol or lower.