Patent ID: 7723121

Claim:
A method for detecting an analyte comprising: causing a redox reaction by contacting a sample containing the analyte with a detection reagent which contains: an enzyme for reducing or oxidizing the analyte; an optional coenzyme; and a compound of the general formula (I) as a fluorimetric redox indicator: wherein R 1 and R 2 each independently selected from R, (CH 2 CH 2 O) m R, COR, COOR and OCOR, R 3 each case is independently selected from NO 2 , CN, R, OR, OCOR, COOR, SR and halogen, R is H or C 1 -C 4 alkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR 2 , COOR, CONR 2 , SO 3 R and salts thereof, and PO(OR) 3 and salts thereof, m is an integer from 1-20, and n is 1, 2 or 3; and performing a fluorimetric determination by irradiating the sample with excitation light of a predetermined wavelength, and detecting the presence of the analyte as a result of the redox reaction and based on the fluorescence emission light emitted by the sample.