Patent ID: 8133486

Claim:
A method for treating a B cell lymphoma in a patient, said method comprising: administering a composition comprising two different proteins to said patient and eliciting an active immune response; wherein (1) the first said protein comprises a full length V H region from said patient and at least a portion of an immunoglobulin constant region, (2) the second said protein comprises a full length V L region from said patient and at least an antigenic portion of an immunoglobulin constant region, and (3) wherein (a) the nucleotide sequences of said V H and said V L region are isolated from a malignant B cell clone from said patient having said B cell lymphoma and wherein said proteins are produced in insect cells wherein V H and said V L sequences are inserted into an expression vector to allow for separate expression of said proteins by said insect cells: and (b) said expression vector has two separate expression cassettes each having a baculovirus promoter and a heterologous secretory signal sequence, wherein (i) said promoters are selected from the group consisting of a p10 promoter and a polyhedrin promoter, and (ii) said secretory signal sequences are selected from the group consisting of human placental alkaline phosphatase secretory signal sequence, honey bee melittin secretory signal sequence, and the endogenous secretory signal sequence associated with the immunoglobulin genes isolated from said patient; and (c) said proteins are produced by introducing said expression vector in insect cells.