Patent ID: 7741455

Claim:
A method for purifying recombinant follicle stimulating hormone (FSH) or a FSH variant comprising subjecting a liquid containing FSH or a FSH variant to the following steps performed in order: (a) subjecting a liquid containing FSH or a FSH variant to anion-exchange chromatography and recovering an eluate containing FSH or FSH variant; (b) subjecting the eluate of step (a) to dye affinity chromatography and recovering an eluate containing FSH or a FSH variant; (c) subjecting the eluate of step (b) to hydrophobic interaction chromatography and recovering an eluate containing FSH or a FSH variant; (d) subjecting the eluate of step (c) to reverse phase chromatography and recovering an eluate containing FSH or a FSH variant; and (e) subjecting the eluate of step (d) to anion-exchange chromatography and recovering an eluate containing FSH or a FSH variant.