Patent ID: 8415099

Claim:
A method of identifying a first nucleotide at a detection position of a target sequence comprising a plurality of detection positions, said method comprising: (a) providing a surface comprising a plurality of concatemers, wherein each concatemer comprises a plurality of monomers and each monomer comprises: i) a first target domain of said target sequence comprising a first set of target detection positions; ii) at least a first adaptor comprising: 1) a first anchor site; and 2) a second adjacent anchor site; b) hybridizing a first anchor probe to said first anchor site; c) hybridizing a second anchor probe to said second anchor site, wherein said second anchor probe also hybridizes to sequences outside said second anchor site; d) hybridizing at least a first sequencing probe to said first target domain, wherein said first sequencing probe comprises: i) a first probe domain complementary to said target domain; ii) a unique nucleotide at a first interrogation position; and iii) a label; under conditions wherein if said unique nucleotide is complementary to a first nucleotide in said first set of target detection positions, said sequencing probe hybridizes to said concatemer; and e) ligating said first anchor probe, said second anchor probe, and said sequencing probe to form a probe ligation products; and; f) detecting said probe ligation product, thereby identifying said first nucleotide.