Patent ID: 8741617

Claim:
A thermostable, family A-type DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the DNA polymerase comprises a motif in the polymerase domain comprising K-L-K-X 2 -T-X 3 -X 4 -D-P-L-P, wherein: X 2 is S or N; X 3 is an amino acid other than Y; and X 4 is V or I (SEQ ID NO:10); wherein the DNA polymerase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:1, wherein the control DNA polymerase has the same amino acid sequence as the DNA polymerase except that the amino acid of the control DNA polymerase at position X 3 is Y.