Patent ID: 7335481

Claim:
A method of screening for one or more compounds that affects an intracellular signal transduction pathway, said method comprising: a) contacting a cell that expresses a protein comprising the amino acid sequence of SEQ ID NO: 2 on its cell surface, and that comprises an intracellular signal transduction pathway that couples activation of the protein comprising the amino acid sequence of SEQ ID NO: 2 to expression of one or more reporter genes, with the one or more compounds under conditions where interaction of a compound with the protein comprising the amino acid sequence of SEQ ID NO: 2 can cause expression of the one or more reporter genes through one or more intracellular signal transduction pathways; b) measuring the level of activation of the protein comprising the amino acid sequence of SEQ ID NO: 2 by the one or more compounds by measuring expression of the one or more of the reporter genes; c) contacting a cell that expresses a protein comprising the amino acid sequence of SEQ ID NO: 2 on its cell surface, and that comprises an intracellular signal transduction pathway that couples activation of the protein comprising the amino acid sequence of SEQ ID NO: 2 to expression of one or more reporter genes, with rosiglitazone under conditions where interaction of a compound with the protein comprising the amino acid sequence of SEQ ID NO: 2 can cause expression of the one or more reporter genes through one or more intracellular signal transduction pathways; d) measuring the level of activation of the protein comprising the amino acid sequence of SEQ ID NO: 2 by rosiglitazone by measuring expression of the one or more of the reporter genes; and e) comparing the level of activation of the protein comprising the amino acid sequence of SEQ ID NO: 2 by the one or more compounds with the level of activation of the protein comprising the amino acid sequence of SEQ ID NO: 2 by rosiglitazone to thereby determine whether the one or more compounds affects the intracellular signal transduction pathway.