Patent ID: 7741090

Claim:
A method for producing glucose dehydrogenase comprising the steps of culturing Burkholderia cepacia having glucose dehydrogenase producing ability in a medium, and collecting glucose dehydrogenase from the medium and/or cells of Burkholderia cepacia , wherein the glucose dehydrogenase has the following properties: (i) the enzyme has an action of catalyzing dehydrogenation reaction of glucose; (ii) the enzyme consists of α subunit(s) having a molecular weight of about 60 kDa and β subunit(s) which is cytochrome C having a molecular weight of about 43 kDa in SDS-polyacrylamide gel electrophoresis under a reducing condition; (iii) the enzyme shows a molecular weight of about 380 kDa in gel filtration chromatography using TSK Gel G3000SW; and (iv) the enzyme shows an optimal reaction temperature around 45° C.