Patent ID: 8632968

Claim:
A method for detecting at least one microorganism in a clinical sample comprising: (a) providing a clinical sample suspected of containing one or more microorganisms; (b) releasing, isolating and/or concentrating polynucleic acids from said at least one microorganism(s) from said clinical sample, if present in the clinical sample; (c) amplifying a portion of said polynucleic acids with the primer pair of SEQ ID NO: 394 and SEQ ID NO: 395, which primer pair amplifies the variable region or a part thereof of the tuf gene from Bordetella pertussis, Chlamydophila pneumoniae, Haemophilus influenza, Legionella pneumophila, Mycoplasma pneumonia, Mycobacterium tuberculosis, Staphylococcus aureus, Staphylococcus epidermis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus mutans, Pseudomonas aureginosa, Enterococcus feacalis or Neisseria meningitides to produce a first detectable amplified product; (d) contacting said first detectable amplified product with at least one probe or combination thereof selected from the group consisting of SEQ ID Nos: 12 to 393 for a time and under conditions sufficient for hybridization to take place; and (e) detecting any hybridization which has taken place in step (d); wherein the presence or absence of hybridization is indicative of the presence or absence of said one or more microorganisms in said clinical sample.