Patent ID: 8462981

Claim:
A computer-implemented method comprising: a. acquiring a first sequence of multiple images of a sample, with each image in the first sequence corresponding to a different spectral weighting function and with the sample comprising multiple components each having a different spectral signature; b. unmixing the first sequence of images into data corresponding to a first set of unmixed images, wherein each unmixed image in the first set corresponds to a spatial distribution in the sample of a different one of the multiple components at a first time; c. acquiring one or more additional images of the sample, with each of the additional images corresponding to a different one of the spectral weighting functions and combining the additional images with one or more of the images in the first sequence to form a second sequence of images; d. unmixing the second sequence of images into data corresponding to a second set of unmixed images, wherein each unmixed image in the second set corresponds to a spatial distribution in the sample of a different one of the multiple components at a second time; and e. displaying information about the sample as a function of time based on the data corresponding to the first and second sets of unmixed images.