Patent ID: 6897067

Claim:
A method, comprising: a) providing: i) a eukaryotic cell; and ii) at least one transfection complex immobilized on a surface, said complex comprising first and second nucleic acids and first and second complexing agents, said first nucleic acid encoding a receptor protein and said second nucleic acid encoding a test protein, wherein said first and second nucleic acid are present in at least one expression vector, and said first complexing agent comprising a ligand for a cell surface receptor that is present on the surface of said eukaryotic cell, and said second complexing agent comprising a DNA binding molecule; and b) contacting said eukaryotic cell with said complex under conditions such that the eukaryotic cell is co-transfected with said first and second nucleic acids and said first and second nucleic acids are expressed; and c) detecting the presence or absence of binding between said receptor protein expressed by said first nucleic acid and said test protein expressed by said second nucleic acid.