Patent ID: 8802429

Claim:
A testing system for determining a quantity of microorganisms, the testing system comprising: a fluorescently detectable product having both acidic and basic species, wherein the fluorescently detectable product is the reaction product of (a) an enzyme substrate that comprises an enzymatically hydrolysable group and a fluorescent group and (b) a test sample comprising a microorganism having an enzyme that hydrolyzes the hydrolysable group from the fluorescent group of the enzyme substrate, and wherein the fluorescently detectable product has an excitation isosbestic point Exλiso where the absorbance of the acid species is the same as the absorbance of the basic species; and a first light source having a wavelength of Exλiso irradiating the fluorescently detectable product, wherein the fluorescently detectable product emits light at a wavelength of Emλ1, and wherein the quantity of light emitted at the wavelength Emλ1 is substantially constant across a pH range of 2.5 to 8.0.