Patent ID: 8163498

Claim:
A method of measuring a molecular weight of hyaluronic acid, comprising at least the following steps (1) to (3): (1) measuring a concentration of hyaluronic acid in a sample; (2) reacting hyaluronic acid in a sample containing the hyaluronic acid with a hyaluronic acid-binding protein, and measuring an amount of the hyaluronic acid-binding protein that is bound to the hyaluronic acid in the sample or a value that reflects the amount; and (3) determining the molecular weight of the hyaluronic acid in the sample from the concentration of the hyaluronic acid in the sample obtained in step (1); and the amount of the hyaluronic acid-binding protein that is bound to the hyaluronic acid in the sample or the value that reflects the amount obtained in step (2), wherein the molecular weight in the sample is determined from a relationship between the molecular weight in a standard hyaluronic acid sample with known concentration and molecular weight and the amount of the hyaluronic acid-binding protein binding to the standard hyaluronic acid sample or the value that reflects the amount.