Patent ID: 8524451

Claim:
A method for the real-time detection of Salmonella in a sample, comprising the steps of: a) providing a sample to be tested for the presence of a Salmonella invA gene target DNA; b) providing a pair of amplification primers that can anneal to the Salmonella invA gene target DNA, wherein the pair of amplification primers is the pair of primers of SEQ ID NOs: 1 and 2; c) providing a probe comprising a detectable label and DNA and RNA nucleic acid sequences that are substantially complementary to the Salmonella target DNA; d) amplifying a PCR fragment between the first and second amplification primers in the presence of an amplifying polymerase activity, amplification buffer; an RNAse H activity and the probe and under conditions where the RNA sequences within the probe can form a RNA:DNA heteroduplex with the complementary DNA sequences in the PCR fragment of the Salmonella target DNA; and e) detecting a real-time increase in the emission of a signal from the label on the probe, wherein the increase in signal indicates the presence of the Salmonella target DNA in the sample.