Patent ID: 8143049

Claim:
An isolated Trichoderma reesei TrCel3A beta-glucosidase comprising one or more amino acid substitutions selected from the group consisting of V66I, S72E, S72N, F96L, V101M, T235S, N248K, N369K, N369P and A386T, wherein the amino acid sequence of the isolated TrCel3A beta-glucosidase comprises a sequence which is from about 90% to about 99.9% identical to wild-type Trichoderma reesei TrCel3A beta-glucosidase encoded by SEQ ID NO:100 and exhibits at least a 2-fold improvement in stability, relative to said wild-type Trichoderma reesei TrCel3A beta-glucosidase when incubated in an aqueous solution at a pH from about 2.0 to about pH 4.5.