Patent ID: 7579157

Claim:
A method for the selection of an antibody against IGF-IR which shows no IGF-IR stimulating activity comprising: a) providing an antibody against IGF-IR; b) preincubating 3T3 cells providing 400,000 to 600,000 molecules of IGF-IR per cell with a medium containing 0.5% heat inactivated fetal calf serum for a period of about 48 hours; c) incubating the 3T3 cells in the presence of the antibody at a concentration of 10 μM; d) measuring PKB phosphorylation in the 3T3 cells incubated in the presence of the antibody; e) incubating the 3T3 cells in the absence of the antibody; f) measuring PKB phosphorylation in the 3T3 cells incubated in the absence of the antibody; g) comparing the PKB phosphorylation in the 3T3 cells incubated in the presence of the antibody to the PKB phosphorylation in the 3T3 cells incubated in the absence of the antibody; and h) selecting the antibody as an antibody which shows no IGF-IR stimulating activity if the PKB phosphorylation in the 3T3 cells incubated with the antibody is the same as the PKB phosphorylation in the 3T3 cells not incubated with the antibody.