Patent ID: 7399619

Claim:
A translation system comprising: a) an orthogonal aminoacyl-tRNA synthetase comprising the amino acid sequence of SEQ ID NO:3 or SEQ ID NO:4; b) an orthogonal aminoacyl-tRNA synthetase comprising an amino acid sequence encoded by the polynucleotide sequence of SEQ ID NO:1 or the polynucleotide sequence of SEQ ID NO:2; or c) an orthogonal aminoacyl-tRNA synthetase comprising an amino acid sequence that is derived from and at least 95% identical to that of SEQ ID NO:10 and that comprises four or more amino acids selected from the group consisting of: i) leucine at a position corresponding to Tyr32 of SEQ ID NO:10; ii) serine or glutamate at a position corresponding to Glu107 of SEQ ID NO:10; iii) proline at a position corresponding to Asp158 of SEQ ID NO:10; iv) leucine or arginine at a position corresponding to Ile159 of SEQ ID NO:10; and v) glutamate or arginine at a position corresponding to Leu162 of SEQ ID NO:10, which synthetase preferentially aminoacylates an orthogonal tRNA with a brominated or iodinated amino acid wherein said brominated or iodinated amino acid is a brominated or an iodinated phenylalanine or tyrosine.