Patent ID: 8663913

Claim:
A method of preparing a lambda phage particle comprising: providing a permissive host bacterial cell comprising an expression system comprising a nucleic acid molecule comprising lambda pR promoter consisting of the nucleotide sequence as set forth in SEQ ID NO:2 and operably linked to a nucleic acid molecule encoding a Shine-Delgarno sequence consisting of the nucleotide sequence as set forth in SEQ ID NO:4 and operably linked to a nucleic acid molecule encoding lambdaphage D gene consisting of the nucleotide sequence as set forth in SEQ ID NO:1, said lambdaphage D gene sequence comprising inserted in frame with the lambdaphage D gene a nucleic acid molecule encoding a gene of interest, said permissive host bacterial cell being suitable for vegetative growth of lambdaphage and further comprising the cl TS 857 repressor; subjecting the host bacterial cell to conditions such that bacteriophage particle expression and assembly is initiated; and recovering the bacteriophage particles from the culture, thereby providing a lambda phage particle.