Patent ID: 6982092

Claim:
A method of preparing a stable preparation of a nucleic acid complexed with a lipid-conjugated polyamide compound, the method comprising: combining a solution of said nucleic acid with a solution of a lipid-conjugated polyamide compound comprising a repeating trimer of monomeric subunits and having the formula: where m is an integer selected from 2 to about 48, Ra is selected from the group consisting of —OH, —H, —SH, —COOH, sulfonyl, a C1-C8 aliphatic group and a C3-C12 aryl, arylalkyl, arylalkenyl, or arylalkynyl group, any of which may include at least one heteroatom selected from the group consisting of nitrogen, sulfur, oxygen, and phosphorus; and a lipid moiety, wherein said lipid moiety may be conjugated to a linker moiety; each R 1 is independently selected from the group consisting of hydrogen, a C1-C8 aliphatic group and a C3-C12 aryl, arylalkyl, arylalkenyl, or arylalkynyl group, any of which may include at least one heteroatom selected from the group consisting of nitrogen, sulfur, oxygen, and phosphorus; and a lipid moiety, wherein said lipid moiety may be conjugated to a linker moiety, wherein at least one group R 1 is not hydrogen; Rc is selected from the group consisting of —OH, —H, —SH, —NH 2 , sulfonyl, hydrazine, a C1-C8 aliphatic group and a C3-C12 aryl, arylalkyl, arylalkenyl, or arylalkynyl group, any of which may include at least one heteroatom selected from the group consisting of nitrogen, sulfur, oxygen, and phosphorus; and a lipid moiety, wherein said lipid moiety may be conjugated to a linker moiety; and each group W is selected from the group consisting of —CH 2 CH 2 —, CH 2 -phenyl-, CH 2 CH 2 O—, —CH 2 CH═CH—, and —CR 2 R 3 —, where R 2 and R 3 are independently selected from the group consisting of hydrogen, a C1-C8 aliphatic group and a C3-C12 aryl, arylalkyl, arylalkenyl, or arylalkynyl group, any of which may include at least one heteroatom selected from the group consisting of nitrogen, sulfur, oxygen, and phosphorus; and a lipid moiety, wherein said lipid moiety may be conjugated to a linker moiety; wherein any said aryl, arylalkyl, arylalkenyl, or arylalkynyl group having fewer than 5 carbon atoms further includes at least one heteroatom; and wherein at least one of said groups Ra, Rc, R 1 for a single monomeric subunit, and W for a single monomeric subunit, comprises a lipid moiety.