Patent ID: 7338793

Claim:
A method of cyclizing a substrate capable of being cyclized by an elisabethatriene cyclase, the method comprising the step of: contacting the substrate with a purified elisabethatriene cyclase under reaction conditions that result in cyclization of the substrate, wherein the purified elisabethatriene cyclase comprises the amino acid sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:5, and has the following characteristics: (A) an apparent molecular weight of about 47,000 Da; (B) an isoelectric point of about 5.1; and (C) the ability to cyclize geranyl geranyl diphosphate, and wherein the substrate is selected from the group consisting of: geranyl geranyl diphosphate, a geranyl geranyl diphosphate analogue, 3-phenyl-3-desmethylgeranylgeranyl diphosphate, farnesyl diphosphate (FPP), FPP isomers, and FPP analogues, and a phosphoisoprenoid.