Patent ID: 8017328

Claim:
A method for partitioning a genome comprising: a) nicking a region of said genome using a sequence-specific nicking endonuclease to produce a nicked double-stranded genomic region comprising a first nick site between a first nucleotide and a second nucleotide; b) hybridizing said nicked double-stranded genomic region with an oligonucleotide comprising: i. an affinity tag; and ii. a nucleotide sequence that is complementary to the nucleotide sequence that is immediately adjacent to said first nick site; to produce a duplex in which a terminal nucleotide of said oligonucleotide lies immediately adjacent to said first nucleotide; c) ligating said terminal nucleotide of said oligonucleotide to said first nucleotide to produce a ligation product; and d) separating said ligation product from unligated products using said affinity tag, thereby partitioning said genome.