Patent ID: 7632669

Claim:
A method of producing a lipolytic enzyme variant comprising: a) selecting a substrate triglyceride comprising a glycerol part having an sn2 position, and an ester bond of interest, b) selecting a parent lipolytic enzyme having an alcohol binding site capable of binding the glycerol part of the substrate triglyceride, c) selecting at least one amino acid residue in the parent lipolytic enzyme which comprises at least one atom within 10 Å of the C atom at the sn2 position of the glycerol part of the substrate triglyceride, d) making an alteration of the at least one amino acid residue of the parent lipolytic enzyme to provide a variant, e) preparing the variant of step d), f) testing the activity of the variant on the selected ester bond, g) selecting a variant having an altered activity on the selected ester bond, and h) producing the selected variant; wherein the parent lipolytic enzyme is a fungal lipolytic enzyme having at least 95% homology with SEQ ID NO:32 or having at least 95% homology with SEQ ID NO:30.