Patent ID: 7785844

Claim:
A composition for detecting HBV target nucleic acids that may be present in a biological sample, comprising: a first primer consisting of SEQ ID NO:22 optionally joined at its 5′ end to a first primer upstream sequence that is not complementary to an HBV nucleic acid, or consisting of SEQ ID NO:26, optionally joined at its 5′ end to a first primer upstream sequence that is not complementary to an HBV nucleic acid; a second primer consisting of SEQ ID NO:23 optionally joined at its 5′ end to a second primer upstream sequence that is not complementary to an HBV nucleic acid; a third primer consisting of SEQ ID NO:15 optionally joined at its 5′ end to a third primer upstream sequence that is not complementary to an HBV nucleic acid; and a fourth primer consisting of SEQ ID NO:11 optionally joined at its 5′ end a fourth primer upstream sequence that is not complementary to an HBV nucleic acid wherein at least two of the primers are joined at their 5′ ends to a primer upstream sequence that is not complementary to an HBV nucleic acid, and wherein the upstream sequence joined to that at least two primers is a promoter sequence for an RNA polymerase, and wherein all four of the primers are in the same solution.