Patent ID: 8728297

Claim:
A method for determining the concentration of glucose in a biological fluid sample from a subject, comprising the steps of: providing a sensor comprising a working electrode, a counter electrode and a sample chamber sized to contain no more than about 1 μL of sample, wherein the sample chamber comprises a redox mediator and an analyte responsive enzyme disposed on the working electrode, the redox mediator comprising: a transition metal selected from the group consisting of osmium, ruthenium, iron, and cobalt; two or more ligands coordinately bound to the transition metal, wherein at least one of the ligands is a monodentate ligand that is a monosubstituted pyridine, and wherein at least one of the ligands is a heterocyclic nitrogen-containing bidentate ligand comprising wherein R 1 is selected from hydrogen, hydroxy, alkyl, alkoxy, alkenyl, vinyl, allyl, amido, amino, vinylketone, keto, or sulfur-containing groups; filling at least a portion of the sample chamber with a biological fluid sample; electrolyzing the biological fluid sample; and determining the concentration of glucose from the electrolyzed sample.