Patent ID: 7579145

Claim:
A method of detecting a target analyte in a test sample, said method comprising: (1) adding a target analyte to a composition comprising an electrode, said electrode comprising: (a) a redox active complex comprising: (i) a redox active molecule, wherein said redox active molecule is a transition metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold; and (ii) a binding ligand, wherein at least one atom of the binding ligand serves as a coordination moiety to said transition metal to form said redox active complex, and wherein said redox active complex is attached to said electrode via a spacer; and (b) a self-assembled monolayer; and (2) detecting said target analyte.