Patent ID: 8822672

Claim:
A method for obtaining a DNA plasmid of interest and comprising the steps of: a) introducing cells containing a DNA plasmid of interest in a passageway and disintegrating the cells continuously by mixing the cells with an alkaline lysis solution to form lysed cells and performing a neutralization step of the said lysed cells with a neutralization solution composed of an acetic acid/acetate composition to form a first mixture; b) performing continuously in the passageway a precipitation of polynucleotide sequence contaminants by adding a solution containing one or more salts to the first mixture of step a) to form a second mixture, wherein the salt(s) are selected from the group consisting of CaCl 2 , MgCl 2 , ZnCl 2 , SrCl 2 , BaCl 2 , LiCl, ammonium acetate, ammonium sulfate, sodium sulfate, magnesium sulphate and a mixture thereof, wherein said solution comprising one or more salts is mixed to said first mixture through Venturi effect and wherein the said one or more salts are at a concentration between 2 M and 6 M; c) recovering the second mixture and allowing a precipitate to separate from the solution of the second mixture, to float and/or to sediment from the solution of the second mixture for a period between about 1 hour and about 48 hours; and d) recovering a soluble material comprising the polynucleotide sequence of interest from the solution of the second mixture, while excluding the precipitate of the second mixture.