Patent ID: 7906110

Claim:
A method of repairing or healing a wound in which the skin is damaged comprising: a) isolating an end population of skeletal muscle-derived progenitor cells (MDCs) by a method comprising: i) plating a suspension of cells from skeletal muscle tissue in a first container, to which fibroblast cells in the muscle tissue suspension adhere; ii) re-plating non-adherent cells from i) in a second container after approximately 15% to 20% of the cells from the cell suspension have adhered to the first container; iii) repeating step ii) at least one time to enrich for an end population of viable, non-fibroblast, desmin-expressing, skeletal muscle-derived myoblasts in the second container; and iv) isolating an end population of viable, non-fibroblast, desmin-expressing skeletal muscle-derived myoblasts in the culture; b) admixing a preparation comprising the MDCs with a physiologically acceptable matrix material to form a stem cell matrix dressing; c) incubating the stem cell matrix dressing in vitro for less than about 12 hours prior to use in dressing a wound in which the skin is damaged; and d) dressing said wound in which the skin is damaged with the stem cell matrix, thereby repairing or healing said wound.