Patent ID: 7432102

Claim:
An isolated transformant producing a substance PF1022 derivative, said transformant is produced by introducing genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid into a phenylalanine auxotrophic host induced from an organism that produces substance PF1022 ([cyclo(D-lactyl-L-N-methylleucyl-D-3phenyllactyl-L-N-methylleucyl)-D-lactyl-L-N-methylleucyl-D-3-phenyllactyl-L-N-methylleucyl)]) represented by formula (I): wherein said genes comprise: (1) a gene encoding 4-amino-4-deoxychorismate synthase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 2; (2) a gene encoding 4-amino-4-deoxychorismate mutase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 4; and (3) a gene encoding 4-amino-4-deoxyprephenate dehydrogenase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 6, and wherein the substance PF1022 derivative is a compound of formula (III) ([cyclo(D-lactyl-L-N-methylleucyl-D-3-(4-nitrophenyl)lactyl-L-N-methylleucyl-D -lactyl-L-N-methylleucyl-D-3-(4-nitrophenyl)lactyl-L-N-methylleucyl)]) represented by the following formula: or a compound of formula (V) ([cyclo(D-lactyl-L-N-methylleucyl-D-3-(4-aminophenyl)lactyl-L-N-methylleucyl-D-lactyl-L-N-methylleucyl-D-3-(4-aminophenyl)lactyl-L -N-methylleucyl)]) represented by the following formula: wherein the phenylalanine auxotrophic host is obtained from Mycelia sterilia by disrupting an endogenous chorismate mutase gene encoding the amino acid sequence of SEQ ID NO: 27 and/or a prephenate dehydratase gene encoding the amino acid sequence of SEQ ID NO: 38 by homologous recombination, “so as to reduce endogenous chorismate mutase and/or prephenate degydratase activity” wherein the Mycelia sterilia is the strain deposited with the National Institute of Advanced Industrial Science and Technology under an accession number of FERM BP-2671.