Patent ID: 8067552

Claim:
A method for separating and purifying a target protein from a cell, in which the target protein is expressed, or a culture broth thereof, wherein sHSPs are added in a process for separation and purification in an amount effective to prevent degradation of the target protein by proteases and wherein the sHSPs are one or more selected from the group consisting of IbpA derived from Agrobacterium tumefaciens , sHSPs derived from Arabidopsis thaliana , HSPB HSPH HSPC and HSPF derived from Bradyrbizobium japonicum , IbpA derived from Brucella suis , sHSPs derived from Bruchnera aphidicola , IbpA derived from Bruchnera aphidicola ( Acyrthosiphon pisum ), sHSPs derived from Citrus tristeza virus, IbpA and IbpB derived from E. coli , IbpB derived from Helicobacter pylori , HSP27 and α,β-crystallin derived from Human, HSP16.5 derived from Methanococcus jannaschii , IbpA derived from Methanopyrus kandleri , HSP25 derived from Murine, sHSPs derived from Mycobacterium leprae , HSP16.3 derived from Mycobacterium tuberculosis , IbpB derived from Pirellula sp., HSP18.1 derived from Pisum sativum (pea), sHSPs derived from Plasmodium falciparum , IbpA derived from the genus Pseudomonas , HSP26 derived from Saccharomyces cerevisiae , IbpA and IbpB derived from Salmonella enterica subsp. enterica serovar Typhi , IbpA and IbpB derived from Salmonella typhimurium , IbpA derived from Shewanella oneidensis , IbpA and IbpB derived from Shigella flexneri , IbpA derived from Sinorhizobium meliloti , IbpA derived from Streotocuccus pyogenes , sHSPs derived from Streptomyces coelicolor , sHSPs derived from Sulfolobus solfataricus , HSP16 derived from Synechococcus vulcanus , IbpA derived from Thermoanaerobacter tengcongensis , IbpA derived from Thermoplasma acidophilum , and IbpA and IbpB derived from Yersinia pestis.