Patent ID: 8529885

Claim:
A method for increasing expression of a therapeutic protein in a joint of a subject with rheumatoid arthritis (RA) consisting of the step of delivering a recombinant AAV5 (rAAV5) virion to a rheumatoid synovial cell in a joint of a subject with RA by injection into the joint, which virion consists essentially of the following components: (a) a capsid protein of AAV serotype 5 (AAV5), and (b) a recombinant adeno-associated virus type 2 (rAAV2) vector comprising a nucleotide sequence encoding a therapeutic protein to which is operably linked a promoter and expression elements; wherein an injection of said rAAV5 virion into said joint results in transduction of the synovial cell and an increase in expression of said therapeutic protein in said subject compared to expression following an injection of an equivalent dose of a virion that comprises (i) said rAAV2 vector of (b) and (ii) an AAV2 capsid protein in a second subject with RA.