Patent ID: 7422897

Claim:
A sustained culture of undifferentiated chicken cells expressing an embryonic stem cell phenotype, the sustained culture comprising: (a) a preconditioned STO feeder matrix; (b) Buffalo Rat Liver (BRL) conditioned media; (c) chicken primordial germ cells and chicken stromal cells, wherein the chicken primordial germ cells and stromal cells are isolated together from the embryonic genital ridge or gonad from a chicken embryo at a stage later than stage 14 according to the Hamburger & Hamilton staging system; and (d) undifferentiated chicken cells expressing an embryonic stem cell phenotype, wherein the undifferentiated chicken cells: (i) are derived from the chicken primordial germ cells isolated from the chicken embryo; (ii) are smaller than the chicken primordial germ cells; and (iii) form one or more colonies of tightly packed undifferentiated chicken cells expressing an embryonic stem cell phenotype.