Patent ID: 8263366

Claim:
A method of creating a steady bioluminescence vector which has DNA sequences listed in SEQ ID NO 1 and used for Gram-negative bacteria comprising steps of: (a) cloning a promoterless luxABCDE that comes from a vector pXen-5 into a promoter-containing vector to express the genes luxABCDE, wherein the gene expression of luxABCDE is controlled through a lacZ promotor of a plasmid pGEM3-Zf+; (b) cloning a conjugative plasmid pSE34 with a plasmid pBlueScript II KS (+/−) that has at least one ColE1 replication origin and at least one drug resistance gene; and (c) ligating said merging the two clones from steps (a) and (b) together so as to obtain the bioluminescence vector which has the DNA sequence listed in SEQ ID NO:1 with the features of auto-bioluminescence, plasmid stability, conjugation and a high copy number.