Patent ID: 7163812

Claim:
A method for producing succinate comprising: (a) providing Actinobacillus succinogenes transformed with a plasmid comprising at least a 1.5 kB PstI-HaeII fragment of a pGZRS-1 replicon so as to be capable of autonomous replication in Actinobacillus succinogenes , at least one selectable marker gene which is expressed in Actinobacillus succinogenes so as to confer resistance to an antibiotic selected from the group of ampicillin, tetracycline, and chloramphenicol, and a recombinant gene expressed under the control of an Actinobacillus succino genes pckA promoter; (b) providing a growth medium for culturing the transformed Actinobacillus succinogenes ; and (c) culturing the transformed Actinobacillus succinogenes in the growth medium to produce the succinate.