Patent ID: 7553666

Claim:
A plant cell culture for producing proteinaceous vaccine antigen comprising: a) a NT-1 cell stably transformed to express a transgene encoding a proteinaceous vaccine antigen comprising SEQ ID NO: 2; and b) a growth medium which supports the growth of said NT-1 cell in culture but which does not support the growth of Mycoplasmataceae and contains no materials of animal origin, said growth medium comprising no materials of animal origin and NT-1 liquid medium comprising: Quantity/L Potassium Phosphate, Dibasic, 3 * H 2 O 180.0 g Sucrose 30.0 g 10× Batch Salts 100 mL 2,4-D (10 mg/ml) 0.11 mL and RO/DI water added to bring the total volume to one liter, said 10× Batch Salt comprising the following compounds added to one liter of RO/DI water: Quantity/L Ammonium Nitrate 16.5 g Boric Acid, Powder 62.0 mg Cobalt Chloride, 6 * H 2 O 0.25 mg Copper (II) Sulfate 5 * H 2 O 0.25 mg EDTA, Disodium, 2 * H 2 O 372.6 mg Iron (II) Sulfate 7 * H 2 O 278.0 mg Manganese Sulfate 1 * H 2 O 169.0 mg Sodium Molybdate, 2 * H 2 O 2.5 mg Potassium Iodide 8.3 mg Potassium Nitrate 19.0 g Potassium Phosphate, Monobasic 1.7 g Zinc Sulfate, 7 * H 2 O 86.0 mg Magnesium Sulfate, Anhydrous 1.807 g Calcium Chloride, Anhydrous 3.322 g Thiamine HCl 10.0 mg Inositol 1.0 g MES, 1 * H 2 O 5.0 g wherein said NT-1 cell is capable of being continuously passaged such that consistent transgene expression is maintained during passaging and wherein said NT-1 cell is capable of being cryopreserved such that consistent transgene expression is recovered upon recovery from cryopreservation.