Patent ID: 8681247

Claim:
A method for use in flattening an image across a usable field of view in an imaging system having an illumination source configured to illuminate a sample platform with illumination at a first frequency and a detector system having a detector that detects illumination at a second frequency by correcting the image for fluorescence spatial variations and scatter spatial variations, the method comprising: acquiring a first image of a stable fluorescent target located proximal to the sample platform using the detector, wherein the stable fluorescent target absorbs illumination at said first frequency and emits at said second frequency and has a size that fills at least a usable field of view of the detector system; acquiring a second image of a scattering target located proximal to the sample platform using the detector, wherein the scattering target scatters illumination at said first frequency and has a size that fills at least the usable field of view of the detector system; determining a pixel remapping function based on the acquired first and second images; and storing the pixel remapping function for later use in correcting for fluorescence spatial variations and scatter spatial variations in images of a sample taken using the imaging system.