Patent ID: 7476514

Claim:
A method of treating a patient that has received a transplant and is being provided with one or more immunosuppressive drugs, comprising the steps of: a) determining a value of an immune response for said patient which reflects activation of CD4 lymphocytes in a whole blood sample stimulated by exposure to phytohemagglutinin (PHA) measured in terms of adenosine triphosphate (ATP) weight in volume of cell lysate of cells isolated from said whole blood sample by immunoselection for CD4 lymphocytes; b) comparing said value with values in a reference set comprising ranges of values of immunological responses for CD4 lymphocytes, wherein said reference set comprises low, moderate and strong ranges of values, wherein values of said low range are less than or equal to 225 nanograms of ATP per mL, values of said moderate range are greater than 225 nanograms of ATP per mL and less than 525 nanograms of ATP per mL, and values of said strong range are equal to or greater than 525 nanograms of ATP per mL; and c) based on a comparison obtained from said comparing step, predicting said patient is at risk of rejection of a transplanted organ if said value compared in said comparing step is in said strong range or ascertaining said patient is at risk of being overmedicated with said one or more immunosuppressive drugs if said value compared in said comparing step is in said weak range.