Patent ID: 8524681

Claim:
A chemically synthesized short interfering RNA comprising: a chemically synthesized passenger oligonucleotide having a length of 15 to 30 nucleotides and comprising: sequence-independent modification format (1): (1) 5′ N p -m-m-N x -m-m-N q -n r 3′, wherein p is 0; x is 10; r is 0, 1, or 2; and q is an integer representing the length of the passenger strand minus (p+x+r+4); wherein each m is independently a bicyclo nucleotide or a tricyclo nucleotide; each n is independently a deoxynucleotide, modified nucleotide, or ribonucleotide; when m is a bicyclo nucleotide, each N is independently a nucleotide other than a bicyclo nucleotide, and when m is a tricyclo nucleotide, each N is independently a nucleotide other than a tricyclo nucleotide and a guide oligonucleotide having 15 to 30 nucleotides including a region of continuous complementarity to the passenger oligonucleotide of at least 12 nucleotides, the guide oligonucleotide further having complementarity to at least a portion of a target mRNA.