Patent ID: 8030544

Claim:
A method for producing a transgenic plant containing at least two heterologous nucleic acid sequences, comprising: (a) providing an explant comprising a first heterologous nucleic acid sequence that confers resistance to an herbicide, an aminoglycoside, or hygromycin; (b) inoculating the explant with Agrobacterium comprising a vector which comprises a second heterologous nucleic acid sequence comprising a selectable marker gene conferring spectinomycin resistance; (c) co-cultivating the explant with the Agrobacterium to obtain an explant that exhibits spectinomycin resistance; and (d) regenerating the explant that exhibits spectinomycin resistance into a transgenic plant containing at least the two heterologous nucleic acid sequences; wherein the explant is treated with a cytokinin at least during steps (b) and (c).