Patent ID: 7393636

Claim:
A method for forming a self-assembly substance of oligonucleotides, said method comprising: (1) providing n (n≧1) dimer-forming probe-bearing groups formed from a first group to a (2n−1)th group in turn, wherein each group includes a plurality of pairs of dimer-forming probes composed of a pair of an oligonucleotide No. 1 and an oligonucleotide No. 2, each oligonucleotide having 3 regions of a 3′ side region, a mid-region and a 5′ side region, in which the mid-regions of the oligonucleotides No. 1 and No. 2 have base sequences complementary to each other, and the 3′ side regions and 5′ side regions thereof have base sequences not complementary to each other; (2) providing n (n≧1) cross-linking probe-bearing groups formed from a second group to a 2 nth group in turn, wherein each group includes a plurality of pairs of cross-linking probes composed of a pair of an oligonucleotide No. 1 and an oligonucleotide No. 2, each oligonucleotide having 2 regions of a 3′ side region and a 5′ side region, in which the 3′ side regions and the 5′ side regions of the oligonucleotides No. 1 and No. 2 have base sequences not complementary to each other, and the cross-linking probes having base sequences capable of cross-linking dimers formed from the dimer-forming probes; and (3) hybridizing the dimer-forming probes of the dimer-forming probe-bearing groups with the cross-linking probes of the cross-linking probe-bearing groups, wherein the oligonucleotides are self-assembled to form the self-assembly substance of oligonucleotides, wherein in the case of n=1, base sequences of the probes are made complementary to each other in the following respective pairs: (a) the 3′ side region of the oligonucleotide No. 1 of the first group of the dimer-forming probe-bearing groups and the 3′ side region of the oligonucleotide No. 1 of the second group of the cross-linking probe-bearing groups; (b) the 5′ side region of the oligonucleotide No. 2 of the first group of the dimer-forming probe-bearing groups and the 5′ side region of the oligonucleotide No. 2 of the second group of the cross-linking probe-bearing groups; (c) the 3′ side region of the oligonucleotide No. 2 of the second group of the cross-linking probe-bearing groups and the 3′ side region of the oligonucleotide No. 2 of the first group of the dimer-forming probe-bearing groups; and (d) the 5′ side region of the oligonucleotide No. 1 of the second group of the cross-linking probe-bearing groups and the 5′ side region of the oligonucleotide No. 1 of the first group of the dimer-forming probe-bearing groups.