Patent ID: 7910357

Claim:
A biochip reader used for reading images of measurement samples by light beam irradiation, comprising: a light source which provides a light which excites a uniform fluorescent plate capable of emitting a fluorescent light which forms uniform fluorescent light distribution on said uniform fluorescent plate and excites a biochip having measurement samples capable of emitting fluorescent light, a measuring camera which captures (i) images generated from fluorescent light emitted by the uniform fluorescent plate and (ii) images generated from fluorescent light emitted by the measurement samples on the biochip, a storage device which stores image data from said measuring camera, an image processing means for correcting the distribution of the quantity of fluorescent light on said biochip according to the following formulas: a i ′=a i ÷a ave (I) c i =b i ÷a i ′ (II) and, a display which displays images processed by said image processing means, wherein a i ′ represents a fluorescent light source intensity correction image value for each individual pixel of said uniform fluorescent plate, a i represents the intensity value of each individual pixel of said uniform fluorescent plate generated by measuring the intensity of each individual pixel of said uniform fluorescent plate with said camera, a ave represents an average value of reference quantity of fluorescent light distribution image of said uniform fluorescent plate generated by averaging a plurality of intensity values of fluorescent images of said uniform fluorescent plate, c i represents a fluorescent light source intensity corrected sample image value for each individual pixel of said biochip, and b i represents the intensity value of each individual pixel of said measurement samples on said biochip generated by measuring intensity of each individual pixel of said measurement samples on said biochip with said camera, and wherein i represents a number of each individual pixel of said uniform fluorescent plate and of said measurement samples on said biochip, and has a value of 1 to n.