Patent ID: 7138267

Claim:
A host constructed using a method for constructing a bacterial host strain which, in the presence of an inducer, expresses a protein or polypeptide required for replication from a multi-copy ori, said method comprising in vivo insertion of a gene for said protein or polypeptide required for replication from a multi-copy ori into the chromosomal DNA of bacterial host by means of a synaptic complex between a transposase and an artificial transposon, said transposon comprising a gene encoding said protein or polypeptide required for replication from said multi-copy ori which is operably joined to an inducible promoter which results in, transcription of said gene and expression of said protein or polypeptide under suitable conditions in the presence of an inducer of said inducible promoter, wherein said artificial transposon comprises an artificial Tn5 transposon comprising hyperactive Mosaic End (ME) sequences and a trfA gene which is operably joined to an inducible araB promoter.