Patent ID: 8252340

Claim:
A method of obtaining an extract, comprising the steps of: (1) cleaning raw material comprising leech and earthworm with water, and then steeping and smashing said raw material for forming homogenate; (2) repeatedly freezing and melting said homogenate, then centrifuging for obtaining supernate; (3) ultrafiltratinq said supernate with an ultrafiltration device of molecular weight exclusion limit of 10,000-100,000 Daltons for obtaining a first filtrate; (4) thermocopressinq said first filtrate at 105-136° C. for 10-45 minutes; (5) ultrafiltrating said filtrate with an ultrafiltration device of molecular weight exclusion limit of less than 6000 Daltons for obtaining a second filtrate; and (6) obtaining from said second filtrate an extract having a molecular weight less than 5800 Daltons.