Patent ID: 7160686

Claim:
A method for producing a specific anti-idiotypic antiembryonic antiserum that specifically binds antigen-stimulated lymphocytes, comprising: i) performing a first immunization by immunizing a rat with a suspension of cells of tissue of a fetus of the same genetic line as the rat that is immunized; ii) recovering spleen cells from said immunized rat and separating lymphocytes therefrom, thus obtaining a lymphocyte suspension; iii) performing a second immunization by immunizing a rat of the same genetic line as the rat that is first immunized with said lymphocyte suspension; iv) recovering an antiserum from said rat immunized in the second immunization; v) adding cells of whole organs of kidney, lung and liver of a normal rat of the same genetic line as the immunized rat to said antiserum, forming a suspension; and vi) separating the supernatant from the sediments from the obtained suspension to obtain the anti-idiotypic antiembronic antiserum that specifically binds antigen-stimulated lymphocytes.