Patent ID: 8609613

Claim:
A method of inhibiting vascular endothelial growth factor (VEGF) biological activity in a cell comprising contacting said cell with a polypeptide that binds human kinase insert domain receptor (KDR) in an amount and for a time sufficient to inhibit said VEGF biological activity; wherein the polypeptide comprises a 10 Fn3 domain between about 80 and about 150 amino acids that has a structural organization comprising: a) at least five to seven beta strands or beta-like strands distributed among at least two beta sheets, and b) at least one loop portion connecting two strands that are beta strands or beta-like strands, which loop portion participates in binding to KDR, wherein the polypeptide binds to an extracellular domain of the human KDR protein with a dissociation constant (K D ) of less than 1×10 −6 M, and wherein the 10 Fn3 domain comprises an amino acid sequence that is at least 60% identical to SEQ ID NO: 5.