Patent ID: 7223585

Claim:
A method of producing virus from a culture of cells, comprising the steps of: (a) providing a culture of mammalian cells in suspension which has been infected by the virus in culture; (b) extracting the virus from the cells by adding a detergent to the culture of mammalian cells in suspension and incubating for a period of time to result in a cell lysate; (c) separating cell debris from the virus in the cell lysate by step-wise filtration comprising: (i) filtering through a prefilter having a pore size of 5 μM or 8 μM, and (ii) filtering after step (i) through a combination filter having pore sizes of 3 μM and 0.8 μM; (d) purifying the virus by a combination of ion exchange and size exclusion chromatography; and (e) collecting the virus, provided that after infection by the virus and before extraction of the virus, the mammalian cells are not pelleted or resuspended.