Patent ID: 8722336

Claim:
A method for detecting in nucleic acid from a sample the presence or absence of an allele of paternal origin, comprising: a) cleaving nucleic acid from the sample with a Tsp509I restriction enzyme; b) exposing the nucleic acid after (a) to amplification conditions that amplify uncleaved nucleic acid but not cleaved nucleic acid, and which amplification conditions are capable of generating amplification products comprising some or all of the single nucleotide polymorphic loci selected from the group consisting of: rs11835780 rs748773 rs7323716 rs4311632 rs6488494 rs9652080 rs13110085 rs1363267 rs10785736 rs13269702 rs10840805 rs7356482 rs1797700 rs2723307 rs7831906 rs2993531 rs4764597 rs9818611 rs1885121 rs4589569 rs12903747 rs1372688 rs2820107 rs7320201 rs1904161 rs6766358 rs4869315 rs1720839 rs12675087 rs3913810 rs10901705 rs7689368 rs6542638 rs6582294 rs725849 rs12450474 rs7144509 rs7900002 rs1346718 rs10234234 rs910500 rs1503660 rs8016543 rs4489023 rs2007475 rs11221881 rs1916803 rs683262 rs13155942 rs10260483 rs10110766 rs494220 rs4488809 rs1041409 rs3912319 rs4533845 rs11099210 rs9428474 rs3816551 rs10754776 rs9929404 rs6556642 rs4130306 rs7294836 rs7205009 rs2734574 rs4673821 rs12674093 rs1401454 rs614004 rs2322301 rs9285190 rs1444647 rs7741525 rs179596 rs7818415 rs17074340 rs331893 rs12007 rs2462049 rs9787011 rs9356029 rs10806232 rs273172 rs6569474 rs11105611 rs9989393 rs10898954 rs12107918 rs1342995 rs6043856 rs664358 rs263025 rs1593443 and rs6142841, thereby generating amplification products; c) analyzing a sufficient number of the single nucleotide polymorphic loci of the amplification products of step (b), whereby at least one of the single nucleotide polymorphic loci analyzed is informative of the presence of an allele of paternal origin; and d) determining the presence or absence of an allele of paternal origin based on the analysis of step (c).