Patent ID: 7601515

Claim:
A process for producing albumin from a yeast culture medium obtained by culturing yeast transformed with an albumin-encoding nucleotide sequence in a fermentation medium and filling a container with the albumin, whereby said yeast expresses and secretes the albumin, the process comprising (a) separating the yeast from the culture medium to thereby yield an albumin solution; (b) subjecting the albumin solution to (1) cation exchange chromatography, wherein albumin is bound to a cation exchange material and then the bound albumin is eluted from the cation exchange material; (2) affinity chromatography, wherein albumin is bound to an affinity chromatography material comprising an albumin-binding compound and then the bound albumin is eluted from the affinity chromatography material; and (3) anion exchange chromatography, wherein albumin is bound to an anion exchange material and the albumin is eluted from the anion exchanger material; (c) concentrating and formulating the albumin with sodium octanoate and sodium chloride; and (d) filling a container with the albumin obtained from step (c).