Patent ID: 8486646

Claim:
A method for identifying a peptide YY (PYY) secretagogue, comprising: (a) contacting in vitro a test compound with a host cell or with a membrane of a host cell comprising a G protein-coupled receptor (GPCR) wherein the GPCR comprises an amino acid sequence selected from the group consisting of: (i) amino acids 1-335 of SEQ ID NO:2; (ii) amino acids 2-335 of SEQ ID NO:2; and (iii) the amino acid sequence of a GPCR encoded by the nucleotide sequence set forth in SEQ ID NO:1; (b) determining the ability of the test compound to stimulate functionality of the GPCR; (c) contacting a compound which stimulates functionality of the GPCR in step (b) with a vertebrate enteroendocrine cell or a cell capable of secreting PYY in vitro; and (d) determining whether the compound stimulates PYY secretion from the vertebrate enteroendocrine cell or the cell capable of secreting PYY, wherein the ability of the test compound to stimulate PYY secretion from the vertebrate enteroendocrine cell or the cell capable of secreting PYY is indicative of the test compound being a PYY secretagogue.