Patent ID: 8431351

Claim:
A method for detecting a bacterium that produces a cell-wall-synthesizing enzyme, PBP2′, comprising detection of PBP2′ via immunochromatography detection based on an antigen-antibody reaction, (i) which uses an immunochromatography detection device comprising on a sheet-like solid-phase support: (a) a sample supply site to which a sample solution is supplied, which sample solution is deduced to contain a bacterium producing PBP2′ or a solution deduced to contain PBP2′ released from the cell wall via sample pretreatment; (b) a labeled reagent site that holds a reagent, which is a labeled antibody binding specifically to PBP2′, in a manner such that the reagent is able to spread across the solid-phase support; and (c) a capture reagent site on which a capture reagent capable of specifically binding to and capturing a complex of PBP2′ and the labeled reagent has been immobilized, the capture reagent site comprising a sulfobetaine-type surfactant, and (ii) which comprises supplying to the sample supply site the sample solution and then evaluating at the capture reagent site the formation of a complex of a labeled reagent/PBP2′/a capture reagent.