Patent ID: 8129157

Claim:
A method to anaerobically ferment glycerol to produce a product, comprising: culturing a bacterial cell in a medium wherein said medium comprises at least 10 g/L glycerol as a carbon source, less than 10 mM potassium, less than 50 mM phosphate, CO2 greater than or equal to 10%, and a pH between 5.0 and 7.5, under anaerobic conditions such that glycerol is converted into a product, wherein said bacterial cell lacks a functional 1,3-propanediol pathway, but has a functional 1,2-propanediol (1,2-PDO) pathway, a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway, and a functional F 0 F 1 -ATPase pathway to couple ATP synthesis with a proton pump, wherein said product is selected from the group consisting of ethanol, succinic acid, formic acid, hydrogen, 1,2-PDO, propanolamides, propionic acid, and butanol.