Patent ID: 8058415

Claim:
A system for simultaneously detecting multiple nucleic acid enzyme cofactors in a sample, comprising: a first nucleic acid enzyme, that reacts to a first nucleic acid enzyme cofactor; a second nucleic acid enzyme, that reacts to a second nucleic acid enzyme cofactor; a first polynucleotide, coupled to a first quantum dot having a first emission wavelength; a second polynucleotide, coupled to a second quantum dot having a second emission wavelength different from the first emission wavelength; at least one quencher, for quenching emissions of the first quantum dot and the second quantum dot, coupled to the first and second nucleic acid enzymes, respectively; a first substrate of the first nucleic acid enzyme; a second substrate of the second nucleic acid enzyme; wherein each of the first substrate and the second substrate is a nucleic acid, and the first polynucleotide and the second polynucleotide are different nucleic acid sequences; the first polynucleotide is hybridized to the first substrate; the second polynucleotide is hybridized to the second substrate; the first nucleic acid enzyme is hybridized to the first substrate and cleaves the first substrate in the presence of the first nucleic acid enzyme cofactor; the second nucleic acid enzyme is hybridized to the second substrate and cleaves the second substrate in the presence of the second nucleic acid enzyme cofactor, the first and second cofactors are different and the first and second nucleic acid enzymes are different.