Patent ID: 7820410

Claim:
A method for producing a preparation of an immunoreactive monoclonal, mouse chimeric or humanized antibody that binds specifically to CD66 and having a greater than 90% antibody immunoreactivity to CD66 comprising the steps of: culturing host cells which produce the immunoreactive antibody in a suitable culture medium by fermentation in a fluidized bed reactor to produce said antibody; harvesting the immunoreactive antibody from one of the host cells or the culture medium, purifying the immunoreactive antibody by means of a reduced column affinity chromatography with protein A, determining the immunoreactive antibody that binds specifically to CD66 by a binding assay using an excess of CD66 antigen, and calculating the percentage of immunoreactive antibody that binds specifically to CD66 using a modified Lindmo test according to the formula: Immunoreactive (IR) [%]=100%−[100%×(antibody-concentration in supernatant/antibody starting concentration)]; said preparation having a greater than 90% antibody immunoreactivity to CD66 is produced.