Patent ID: 7276597

Claim:
A composition comprising: (i) one or more template nucleic acids, each of which comprises in 3′ to 5′ order a first region, an extension region, and a second region; (ii) a first oligonucleotide that is at least partially complementary to said first region of said template nucleic acid; and (iii) one or more second oligonucleotides corresponding to said one or more template nucleic acids, each of said second oligonucleotides comprising a 5′ region and a 3′ region, wherein each said 3′ region is at least partially complementary to said second region of said corresponding template nucleic acid and wherein each said 5′ region is at least partially complementary to a region that may be upstream of, downstream of, or comprise said first region of said corresponding template nucleic acid.