Patent ID: 8039815

Claim:
A fluorescent light microscope for measuring a sample, the fluorescent light microscope comprising: a light source providing transfer light having at least one transfer wavelength for transferring a fluorescent dye in a sample to be measured from one state into another state, the light source comprising at least one pulsed laser, an optical wave guide comprising a single-mode fiber that is connected to the at least one pulsed laser, and a wavelength-selective device connected to the optical wave guide, the at least one pulsed laser emitting pump light of a pump wavelength other than the transfer wavelength and injecting the pump light into the optical wave guide as high peak power pulses, the high peak power pulses of the pump light, due to a non-linear effect in the optical wave guide, generating a light spectrum emerging from the optical wave guide, and the wavelength-selective device singling out the transfer light by its transfer wavelength from the light spectrum emerging from the optical wave guide, wherein the pulses have a duration in a range from about 0.5 to about 5 ns, wherein the optical wave guide is selected in such a way and the at least one pulsed laser injects the pump light into the optical wave guide at such an intensity that Raman scattering is stimulated in the optical wave guide at such an extent that the light spectrum emerging from the optical wave guide, besides the pump wavelength, comprises at least one red-shifted Stokes line whose full width at half maximum is smaller than half of its distance to its next neighbour line on the blue side of the spectrum, and wherein the wavelength selective device singles out the transfer light from the red-shifted Stokes lines of the light spectrum, wherein light emerging from the optical wave guide is pulsed, wherein i the light emerging from the optical wave guide have about the same duration as the duration of the pulses; an excitation light source which provides excitation light for exciting the fluorescent dye for fluorescence, the excitation light source comprising a laser diode triggered synchronously with the at least one pulsed laser; and a detector which measures fluorescent light from the sample with spatial resolution.