Patent ID: 8609418

Claim:
A method for regenerating plants of Jatropha curcas comprising: (a) culturing cotyledon explants on a solid callus formation medium which comprises MS mineral salts, B5 vitamins, citric acid, glutamine, casein hydrolysate, sucrose, 6-benzylaminopurine (6-BA) and 1-naphthaleneacetic acid (NAA) for a period of time to produce explants with callus tissue; (b) culturing the callus tissue on a solid first shoot regeneration medium which comprises MS mineral salts, B5 vitamins, citric acid, glutamine, casein hydrolysate, sucrose, adenine, 6-BA and 3-indolebutyric acid (IBA) for a period of time to produce callus tissue with shoots and callus tissue; (c) culturing the shoots on a solid second shoot regeneration medium which comprises MS mineral salts, B5 vitamins, citric acid, glutamine, casein hydrolysate, sucrose, 6-BA, IBA and gibberellic acid (GA 3 ) for a period of time to produce shoots; (d) culturing shoots from step (c) on a solid shoot elongation medium which comprises MS mineral salts, B5 vitamins, citric acid, glutamine, casein hydrolysate, sucrose, 6-BA and GA 3 for a period of time to produce elongated shoots; and (e) rooting or grafting the elongated shoots, wherein rooting is performed by culturing the elongated shoots on a solid rooting medium which comprises MS mineral salts, B5 vitamins, sucrose and IBA for a period of time to produce plantlets or wherein grafting is performed by grafting the elongated shoots to J. curcas rootstock.