Patent ID: 8431416

Claim:
A dye-conjugate having the formula: wherein X is O or S; R 1 , R 2 , R 3 , R 4 , R 10 , R 11 , and R 12 are independently H, halogen, alkyl, alkoxy, aryloxy, thiol, alkylthiol, arylthiol, azido, amino, hydroxy, boronic acid, WSG, or substituted alkyl or substituted alkoxy, wherein the substituted alkyl or substituted alkoxy is substituted one or more times by halogen, amino, hydroxy, carbonyl, boronic acid, or WSG; WSG is a water-soluble group, wherein WSG is a sulfonate, a thiosulfonate, a phosphonate, a boronate, an ammonium, a pyridium, a quinolium or an acridinium; n is an integer of 1-35; SUB is a substrate, wherein SUB is a biopolymer; at least one of R 10 , R 11 , and R 12 is covalently bound to the SUB through an optional linker L, wherein L is none, an alkyl, alkoxy, a thioalkyl, an amino acid, a sulfo amino acid, polyamine, a polyethyleneglycol, an aryl, or a heteroaryl; and at least one of R 10 , R 11 , and R 12 is WSG.