Patent ID: 6897016

Claim:
Method for splicing a target RNA molecule comprising a mutant beta-globin nucleotide sequence within a cell in culture with a separate RNA molecule comprising a wild type beta-globin nucleotide sequence, wherein a protein product of the target RNA molecule is deleterious to the cell in which it is located, and wherein the separate RNA molecule is adapted to form a target RNA molecule with the wild type beta-globin nucleotide sequence in place of mutant beta-globin nucleotide sequence when spliced with at least a part of the target RNA molecule, the method comprising: contacting the target RNA molecule with a catalytic RNA molecule comprising the separate RNA molecule, under conditions in which at least a portion of the separate RNA molecule is spliced with at least a portion of the target RNA molecule to form the target RNA molecule with the wild type beta-globin nucleotide sequence in place of mutant beta-globin nucleotide sequence when spliced with at least a part of the target RNA molecule.