Patent ID: 8842264

Claim:
An apparatus for performing a target analyte immunoassay of a fluid sample including a detectable label, which label is operative to bind to the target analyte to produce labeled target analyte, which labeled target analyte is detectable in the sample, the apparatus comprising: a sample chamber having at least one first surface area that has target analyte specific ligands attached to it, and at least one second surface area that is free of target analyte-specific ligands operative to selectively bind target analyte present in the sample to the second surface area; and an imaging device adapted to optically scan the at least one first area to determine a label signal intensity value per unit distribution of the labeled target analyte in the at least one first area, and adapted to optically scan the at least one second area to determine a label signal intensity value per unit distribution of the labeled target analyte in the at least one second area, and adapted to determine at least one of an amount of labeled target analyte in the at least one first area, an amount of labeled target analyte in the at least one second area, or a ratio of labeled target.