Patent ID: 8580577

Claim:
A method for detecting an analyte in a sample comprising, in a homogeneous format, the steps of providing detection probes labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support labeled with a second reporter different than the first reporter, providing capture probes bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection comprises recording an image of the sample at an emission wavelength of the second reporter simultaneously with an image used for detecting the detection probes, generating a mask obtained from imaging the sample at the emission wavelength of the second reporter and applying this mask to the image of the sample used for detecting the detection probes.