Patent ID: 7214546

Claim:
A chemiluminescent substrate delivery system represented by a formula selected from the group consisting of: wherein: “Linker” represents a linker moiety; “Dendrimer” represents a dendrimer moiety resulting from the reaction of a surface functional group on the dendrimer with a functional group on the linker moiety wherein said dendrimer moiety comprises chemiluminescent enhancing moieties selected from the group consisting of albumins, water soluble polymeric quaternary ammonium salts, water soluble polymeric quaternary sulfonium salts, water soluble polymeric quaternary phosphonium salts and mixtures thereof; N is a positive integer representing the number of chemiluminescent substrates conjugated to the dendrimer moiety; Y is H, a hydroxyl group, a halogen, an unsubstituted alkyl group, a hydroxy substituted alkyl group, a halogen substituted alkyl group, a phenyl group, a halogenated phenyl group, an alkoxy substituted phenyl group, an alkoxy phenoxy group, a hydroxy alkoxy group, a cyano group, an amide group, an alkoxy group or a carboxyl group; R is a C 1 –C 12 alkyl, mono-, di-, or trihaloalkyl, an aryl or an aralkyl; X is an enzyme-labile group selected from the group consisting of a phosphate, galactoside, acetate, 1-phospho-2,3-diacylglyceride, 1-thio-D-glucoside, adenosine triphosphate, adenosine diphosphate, adenosine monophosphate, adenosine, α-D-glucoside, β-D-glucoside, β-D-glucuronide, β-D-mannoside, β-D-mannoside, β-D-fructofuranoside, β-glucosiduronate, 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranoside, alkoxy derivatives of 5-acetamido-3,5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranoside, p-toluenesulfonyl-L-arginine ester, and p-toluenesulfonyl-L-arginine amide; Z is a halo, alkoxy or alkyl group; and T is H, an electron donating group, an electron withdrawing group, or an organic linker group which may be attached to an ancillary fluorophore or to a biological moiety.