Patent ID: 8163548

Claim:
A live, replication-competent, recombinant, attenuated yellow fever virus (YFV), comprising, in order from 5′ to 3′: i) a 5′ untranslated region (UTR) from a parent YFV; ii) an open reading frame (ORF) encoding all viral proteins of the parent YFV; iii) an internal ribosome entry site (IRES); iv) an exogenous nucleic acid insert that encodes a polypeptide from a virus that is a member of arenaviridae, paramyxoviridae, orthomyxoviridae, hepadnaviridae, herpesviridae, or poxviridae; and v) a 3′ UTR from the parent YFV, wherein the recombinant YFV is capable of propagating in a mammalian host, and wherein the parent YFV is strain 17D, and wherein the nucleic acid insert is retained for at least three passages of the recombinant YFV.