Patent ID: 7715990

Claim:
A method of correcting oligo probe hybridization signals, the method comprising: (a) deriving a correction coefficient for an oligo probe using genomic DNA hybridizations to a first microarray, comprising: (i) measuring signals from each oligo probe during multiple genomic DNA hybridizations to the first microarray, wherein the multiple genomic DNA hybridizations are within a linear range; and (ii) calculating a correction coefficient for each oligo probe based on the measured signals from the genomic DNA hybridizations according to the equation C i =1/S i , where C i is the correction coefficient and S i is the average of the signals for each probe observed in step (a)(i); and (b) correcting oligo probe hybridization signals from a second microarray for determining gene expression, comprising: (i) employing one or more of the oligo probes with calculated correction coefficients from (a) in the second microarray for determining gene expression; and (ii) correcting the signal for each oligo probe on the gene expression microarray using the calculated correction coefficient, wherein the corrected oligo probe hybridization signals derived from the gene expression microarray are outputted to a computer memory.