Patent ID: 8053213

Claim:
A method for analyzing nucleic acids in a sample containing DNA comprising the steps of adding a DNA binding dye to the sample to bind the nucleic acids in the sample and form a nucleic acid/DNA binding dye complex, wherein the DNA binding dye is RO 27 having a structure: amplifying the nucleic acids by means of a polymerase chain reaction (PCR), applying the amplified sample to a gel matrix, separating the amplified nucleic acid molecules according to size by applying a voltage to the gel matrix, exciting the molecules with light at a wavelength suitable for visualizing the dye complex, and optically visualizating the nucleic acid/DNA binding dye complex, thereby analyzing the nucleic acids in the sample, wherein the gel matrix is not pre-stained prior to separating the nucleic acid molecules and wherein the gel matrix is not stained after separating the nucleic acid molecules.