Patent ID: 8748119

Claim:
A method of determining calcineurin activity comprising the steps of: i) contacting in a reaction mix a first test sample and a fluorescently-labeled phosphorylated peptide substrate capable of being dephosphorylated by calcineurin, under conditions such that calcineurin dephosphorylates the fluorescently-labeled phosphorylated peptide; and ii) contacting the reaction mix with a TiO2 matrix, thereby partitioning fluorescently-labeled phosphorylated peptide from fluorescently-labeled non-phosphorylated peptide providing a partition with fluorescently-labeled non-phosphorylated peptide; and iii) determining calcineurin activity wherein measuring an amount of fluorescence emitted by the partition with fluorescently-labeled non-phosphorylated peptide is an indication of calcineurin activity.