Patent ID: 7588899

Claim:
A method for the identification of a potential kinase inhibitor agent comprising the steps of: a) establishing the K d of an active site probe for an ATP binding site, wherein the probe is a fluorescent probe; b) contacting the active site probe with a protein comprising a kinase domain and measuring a signal from said probe to establish a base line level signal; c) incubating the active site probe: kinase complex with a candidate inhibitor agent and measuring the signal from said probe; d) comparing the signal from step b) with the signal from step c); and e) deriving the K d for the candidate inhibitor agent based on the K d of the probe for its ATP binding site and the dose response of the candidate inhibitor agent; under conditions where the probe can be displaced by the candidate inhibitor agent and wherein the active site probe binds to the ATP binding domain of said kinase, wherein the signals are measured as a shift in fluorescent polarization of the probe.