Patent ID: 7932088

Claim:
A method for genetically modifying a Plasmodium parasite by insertional mutagenesis comprising: (a) preparing a minimum piggyBac transposon vector comprising a minimal piggyBac transposon containing a human dihydrofolate reductase (hdhfr) coding sequence under the control of a Plasmodium 5′ calmodulin regulatory element and a 3′ histidine rich protein-2 regulatory element; (b) preparing a helper plasmid comprising a piggyBac transposase coding sequence; (c) isolating a preparation of blood-stage Plasmodium parasites; and (d) introducing said minimum piggyBac transposon vector and said helper plasmid to said preparation of blood-stage Plasmodium parasites to provide a transformed Plasmodium parasite, wherein said piggyBac transposase coding sequence encodes a protein sequence provided by SEQ ID NO: 2.