Patent ID: 7598032

Claim:
A method of DNA sequencing which comprises: a) providing a thermostable DNA polymerase characterized in that i) said thermostable DNA polymerase comprises: the amino acid sequence LeuSerXaaXaaLeuXaaXaaProXaaXaaGlu (SEQ ID NO: 1), whereby “Xaa” at positions 3, 9, and 10 of said sequence are any amino acid residue, “Xaa” at position 4 is Lys, “Xaa” at position 6 is Ala or Ser and “Xaa” at position 7 of said sequence is Ile, and ii) said thermostable DNA polymerase has a level of discrimination against incorporation of nucleotides labeled with a cyanine dye which is reduced in comparison to a polymerase whose sequence is identical to that of said thermostable DNA polymerase except that “Xaa” at position 4 is Glu; b) providing a dye-terminator labeled with a cyanine dye; and c) performing a dye-terminator sequencing reaction.