Patent ID: 7420674

Claim:
A method for the analysis of fluorescing samples in an image-generating microscope system, including a laser scanning microscope, comprising the steps of: scanning a sample point-by-point or line-by-line in at least one surface section; carrying out dispersive splitting of radiation coming from the sample during the scanning; detecting the split radiation by at least one line of detector elements in a wavelength-dependent manner; carrying out a selection of two-dimensional or three-dimensional sample parts which correspond to pre-stored two-dimensional or three-dimensional geometric objects or the like based on recorded and stored intensity distribution of at least one of said detector elements and/or at least one other detector element for the radiation emitted from the sample by image processing; carrying out an analysis of the spectral signature and/or spatial spectral sequence to produce a result for at least a portion of these sample regions with respect to the fluorescence markers arranged thereon; and providing said result as an output to a user and/or for storage for later use and/or to be displayed by a user; and wherein the time-dependent geometric and/or spectral behavior of the sample regions are/is determined.