Patent ID: 7972778

Claim:
A method for detecting a target nucleic acid molecule in a sample, said method comprising: providing a sample containing at least one molecule of a target nucleic acid and constituents for enabling amplification of the target nucleic acid molecule; loading the sample into a sample chamber comprising a flow through channel, said sample chamber including a device for retaining a reaction product of amplification of the target nucleic acid; subjecting the sample in said sample chamber to a homogeneous amplification assay comprising at least a first round of amplification under conditions such that amplification of said target nucleic acid occurs and a reaction product of the amplification of a single molecule of the target nucleic acid comprises a clonal amplified product having a detectable concentration within a portion of said sample chamber after a single round of amplification; and detecting the clonal amplified product of said target nucleic acid after said first round of amplification.