Patent ID: 8663950

Claim:
A method for producing an isolated humanized E16 antibody (hu-E16) and fragments thereof against West Nile Virus (WNV), comprising: a) transfecting a N. benthamiana plant cell with a nucleic acid molecule having SEQ ID NO:4, which encodes a heavy chain sequence of hu-E16, and a nucleic acid molecule having SEQ ID NO:6, which encodes a light chain sequence of hu-E16, wherein said heavy and light chain sequences of hu-E16 comprise N. benthamiana -optimized codons; b) expressing said heavy and light sequences of hu-E16 in said transfected N. benthamiana plant cell; c) growing said N. benthamiana plant; and d) purifying the hu-E16 from the leaves of said N. benthamiana plant, wherein said hu-E16 neutralizes WNV in vitro and in vivo.