Patent ID: 7867703

Claim:
A method of nucleic acid sample analysis, comprising: a) fragmenting a sample of genomic DNA from a cell to obtain fragmented double-stranded genomic nucleic acids, wherein said fragmenting is done by shearing, nebulizing or sonicating; b) ligating, using DNA ligase, a double-stranded adaptor duplex comprising a primer site to both ends of said fragmented double-stranded genomic nucleic acids to produce adaptor-ligated nucleic acids; c) hybridizing said adaptor-ligated nucleic acids with a solid support having nucleic acid probes bound thereto to result in complementary fragments being retained on the solid support via the probes; d) washing the solid support to provide a washed solid support comprising said complementary fragments; e) subjecting said washed solid support to conditions sufficient to separate all complementary fragments retained on said washed solid support via said probes from the washed solid support to produce a reduced complexity mixture that is made up of said complementary fragments; f) amplifying said complementary fragments of said mixture using a primer that binds to said primer site of the adaptor duplex at both ends of said complementary fragments to produce an amplified nucleic acid sample comprising amplified nucleic acids; and g) sequencing said amplified nucleic acids.