Patent ID: 8765436

Claim:
A thermostable, family A-type DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the DNA polymerase comprises a motif in the polymerase domain comprising E-R-M-A-F-X 3 -M-P-V-Q-G-T-A-A-D, wherein: X 3 is any amino acid other than N (SEQ ID NO:10); wherein the DNA polymerase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:1, 2, 3, 4, 5, 6, or 7, and wherein the control DNA polymerase has the same amino acid sequence as the DNA polymerase except that the amino acid of the control DNA polymerase at position X 3 is N.