Patent ID: 8795737

Claim:
A method of making nanoceria particles functionalized with a human carbonic anhydrase II inhibitor, the method comprising: (a) preparing amine functionalized cerium oxide nanoparticles by (i) reacting cerium oxide and epichlorohydrin in an aqueous sodium hydroxide solution to form a cerium oxide nanoparticle reaction product, (ii) removing the cerium oxide nanoparticle reaction product from the aqueous sodium hydroxide solution, and (iii) placing the removed cerium oxide nanoparticle reaction product into an ammonium hydroxide solution; (b) attaching the human carbonic anhydrase II inhibitor to the amine functionalized cerium oxide nanoparticles by (i) adding NMP and BOP to a solution of the human carbonic anhydrase II inhibitor to form a reaction mixture, (ii) mixing the amine functionalized cerium oxide nanoparticles with the reaction mixture, and (iii) separating nanoceria particles functionalized with the human carbonic anhydrase II inhibitor.