Patent ID: 8663912

Claim:
A probe comprising: an oligopeptide consisting of no more than 80 amino acids of sequence of von Willebrand Factor (VWF), said oligopeptide comprising a scissile Y1605-M1606 peptide of a VWF sequence set forth as SEQ ID NO: 3, a cysteine substitution located from 1 to 12 amino acids from the scissile Y-M in the carboxy terminal direction, and a C-terminal segment; a fluorophore: and a fluorescence quencher, wherein the fluorophore and the fluorescence quencher are bound to the oligopeptide on opposite sides of the scissile Y-M peptide and wherein the oligopeptide comprises a substitution of lysine K1617 of a VWF sequence set forth as SEQ ID NO: 3 with an amino acid that does not comprise a primary amine on its side chain.