Patent ID: 7332169

Claim:
A method for generating infectious copy avian paramyxovirus, wherein said avian paramyxovirus infectious copy is derived from Newcastle Disease Virus, the method comprising: transfecting at least one cell with an avian-paramyxovirus cDNA comprising a nucleic acid sequence corresponding to the 5′-terminal end of the genome of avian paramyxovirus, as set forth in SEQ ED NO:82, and, further corresponding to the 3′-terminal end of the genome of avian-paramyxovirus, as set forth in SEQ ID NO:80 or SEQ ID NO:81, and further comprising a nucleic acid encoding a heterologous protein, wherein said heterologous protein is derived from a poultry pathogen selected from the group consisting of Avian Influenza, Avian leukosis virus, Chicken anemia virus, Marek's disease virus, Infectious laryngotracheitis virus, Infectious bursal disease virus, Turkey rhinotracheitis virus, Infectious bronchitis virus, Reoviruses, Adenoviruses, Pneumoviruses, Salmonella enteritidis, Campylobacter jejuni, Escherichia coli, Bordetella avium, Haemophilus paragallinarum, Pasteurella multocida, Ornithobacterium rhinotracheale, Riemerella anatipestifer , and Mycoplasma, thus generating an infectious copy of avian paramyxovirus.