Patent ID: 8460875

Claim:
A RPA process of DNA amplification of a double stranded target sequence, the target sequence comprising a first strand of DNA and a second strand of DNA, the RPA process comprising: (a) contacting a recombinase agent with a first nucleic acid primer and a second nucleic acid primer to form a first nucleoprotein primer and a second nucleoprotein primer; (b) contacting the first and second nucleoprotein primers with the double stranded target sequence thereby forming a first double stranded structure at a first portion of the first strand and a double stranded structure at a second portion of the second strand such that the 3′ end of each of the first nucleic acid primer and the second nucleic acid primer are oriented toward each other on the same template nucleic acid molecule; (c) extending the 3′ end of each of the first and second nucleoprotein primers with one or more polymerases and dNTPs to generate a first double stranded nucleic acid, a second double stranded nucleic acid, a first displaced strand of nucleic acid, and a second displaced strand of nucleic acid; and (d) repeating (b) and (c) to amplify the double stranded target sequence, wherein each of the first and second nucleic acid primers is partially single stranded and partially double stranded, and each of the first and second nucleic acid primers comprises a longer invading-strand which possesses a specific 3′ primer sequence and a shorter non-invading strand which is complementary to the 5′ end of the longer invading strand.