Patent ID: 7892784

Claim:
A method for manufacturing a lactam ring-opened streptothricin derivative or salt thereof, comprising a step of: contacting a protein with a substance containing a lactam ring to open the lactam ring, wherein said protein is encoded for by the polynucleotide comprising at least one of (a) through (d) below: (a) a polynucleotide containing a polynucleotide comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 and 15; (b) a polynucleotide containing a polynucleotide encoding for a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 and 16; (c) a polynucleotide containing a polynucleotide encoding for a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 and 16 with 1-10 of amino acids deleted, substituted, inserted and/or added and having lactam ring-opening activity; and (d) a polynucleotide containing a polynucleotide encoding for a protein having an amino acid sequence that has 90% or more identify with an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 and 16, and having lactam ring-opening activity.