Patent ID: 7604958

Claim:
A method for the production of α-N-acetylglucosaminidase, said method comprising: (i) transfecting mammalian cells with an expression vector comprising a DNA molecule encoding α-N-acetylglucosaminidase having the amino acid sequence of SEQ ID NO:2 or fragment thereof comprising amino acids 24-743 of SEQ ID NO:2 fused to a signal sequence; (ii) culturing the transfected cells, whereby the α-N-acetylglucosaminidase protein having the amino acid sequence of SEQ ID NO:2 or fragment thereof comprising amino acids 24-743 of SEQ ID NO:2 fused to a signal sequence is produced and α-N-acetylglucosaminidase protein secreted into the medium; and (iii) recovering the α-N-acetylglucosaminidase protein, from the medium.