Patent ID: 8877892

Claim:
A method of producing a reagent for use in a conjugation reaction comprising a) forming a homogeneous solution or suspension of i) a carboxyl-activating or phosphate-activating non-enzymatic substance comprising 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-Cyclohexyl-N′-(β-[N-methylmorpholino]ethyl)carbodiimide (CMC), 1-ethyl-3-(4-azonia-4,4-dimethylpentyl)carbodiimide (EAC), 2,2-dichloro-5-(2-phenylethyl)-4-(trimethylsilyl)-3-furanone (DPTF), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM), or N-ethyl-5-phenylisoxazolium-3′-sulphonate (Woodwards reagent K (WRK)), wherein the substance is capable of acting on a carboxyl group- or phosphate group-containing moiety to facilitate formation of (1) an amide bond between a carboxyl group and an amine group or (2) a phosphoramidate bond between a phosphate group and an amine group, and ii) a first reactant containing a carboxyl group or a phosphate group, or a second reactant containing an amine group; and b) drying the solution or suspension to produce the reagent comprising a homogeneous mixture of i) and ii).