Patent ID: 8486266

Claim:
A method of wastewater remediation of a volume of wastewater stored in a reservoir, comprising the steps of: a) providing a vessel having an interior holding a volume of nutrient liquid medium; b) adding a volume of bacteria to the nutrient liquid medium; c) providing a fluid transfer system that is in fluid communication with the vessel, the transfer system including an influent flow line and an effluent flow line; d) for a first time interval, recirculating the combination of nutrient liquid medium and bacteria through a selected flow path; e) after the first time interval, transmitting a volume of the combination of nutrient liquid medium and bacteria to the reservoir; and f) wherein in step “d” the bacteria concentration increases to a concentration of between about 1.0×10 7 to 1.0×10 10 cfu (colony forming units) per milliliter (ml).