Patent ID: 7919278

Claim:
A method of amplifying nucleic acid from a cell comprising: contacting a sample comprising a cell and polyanethole sulfonate with a nonplanar solid substrate in a liquid medium having a pH in a range of 3.0-6.0 to attach the cell to the nonplanar solid substrate, wherein the nonplanar solid substrate is hydrophobic and has a water contact angle of 70° to 95° or the nonplanar solid substrate has at least one amine-based functional group at its surface prepared by coating the nonplanar solid substrate with polyethyleneiminetrimethoxysilane (PEIM); washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing a polymerase chain reaction (PCR) using the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and performing the PCR are performed in a single vessel and the cell is a microorganism cell.