Patent ID: 8734774

Claim:
A method of treating a subject with a cancer, the method comprising: (a) selecting a subject with cancer; and (b) administering to the subject an effective amount of a chimeric polypeptide, wherein the chimeric polypeptide comprises (i) a first polypeptide comprising an interleukin-2 (IL-2) polypeptide or a fragment of an IL-2 polypeptide; (ii) a second polypeptide comprising a protease-cleavable sequence; and (ii) a third polypeptide comprising a blocking polypeptide, wherein the blocking polypeptide blocks the activity of the IL-2 polypeptide or fragment of the IL-2 polypeptide, wherein the protease cleavable sequence is cleaved by PSA or a matrix metalloproteinase (MMP), wherein the cancer is selected from the group consisting of prostate cancer, lung cancer, colon cancer, breast cancer and skin cancer and wherein the chimeric polypeptide is selected from the group consisting of SEQ ID NO: 31; SEQ ID NO: 32; SEQ ID NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37; SEQ ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; a chimeric polypeptide comprising SEQ ID NO: 45, wherein amino acids 154-171 of SEQ ID NO: 45 are replaced with amino acids 170-201 of SEQ ID NO: 38; and a chimeric polypeptide comprising SEQ ID NO: 48, wherein amino acids 154-176 of SEQ ID NO: 48 are replaced with amino acids 170-201 of SEQ ID NO: 38.