Patent ID: 7327459

Claim:
A fluorescence detector for detecting in real time PCR amplification occurring in a microfluid chip having a micro chamber with a predetermined volume, the fluorescence detector comprising: a light source generating an excitation beam; a first optical system capable of irradiating the excitation beam having a predetermined spot size to the micro chamber; a first detector; and a second optical system reflecting a fluorescent beam derived from the excitation beam having the predetermined spot size in the micro chamber, to the first detector, wherein the first optical system comprises: a first filter transmitting a short wavelength component of the excitation beam; a first lens disposed between the light source and the first filter and collecting the excitation beam; a first mirror transmitting a predetermined wavelength component of the excitation beam transmitted by the first filter and reflecting the fluorescent beam derived in the micro chamber; and an objective lens making the excitation beam transmitted by the first mirror have the predetermined size.