Patent ID: 8734630

Claim:
A method for separating analytes by capillary electrophoresis comprising: providing a capillary electrophoresis apparatus having at least one capillary; inserting into the at least one capillary a composition comprising: (a) a sieving component comprising an acrylamide polymer having a weight average molecular weight greater than about 3,000,000 Da and less than or equal to about 6,400,000 Da; and (b) a surface interaction component comprising one or more polymers selected from the group consisting of poly(meth)acrylamide, N,N-disubstituted polyacrylamide and N-substituted polyacrylamide, wherein said N-substituents are selected from the group consisting of C 1 to C 3 alkyl, halo-substituted C 1 to C 3 alkyl, methoxy-substituted C 1 to C 3 alkyl, and hydroxyl-substituted C 1 to C 3 alkyl; wherein: the sieving component and the surface interaction component differ in polymer chemical composition; and the composition does not include a crosslinked polymer gel; loading a sample of different sized analytes into the at least one capillary; and separating the analytes by capillary electrophoresis.