Patent ID: 7439073

Claim:
A method of detecting biochemical substances which comprises the steps of: preparing a substrate, an optical film having a refractive index of 1.8 or more formed on said substrate, and a sensor chip comprising a first biochemical substance formed on said optical film; supplying to said sensor chip an aqueous solution containing a second biochemical substance which is bound with said first biochemical substance; irradiating said sensor chip with light with an optical fiber; detecting the reflected light from said sensor chip with said optical fiber while it is in a pre-dried state; and determining a wavelength that provides minimal reflectance, wherein the minimal reflectance of the wavelength is due to attenuation of reflection by an interference effect of said optical film, and wherein said optical fiber is secured to a movable block provided with a stop mechanism, and said stop mechanism is configured to reproducibly position said optical fiber relative to said optical film, and wherein a window is provided between said optical fiber and said sensor chip, and a liquid is provided between said optical fiber and said window.