Patent ID: 7700334

Claim:
A method for producing a surface for immobilizing a polypeptide of interest to the surface wherein the polypeptide of interest is fused to an N-intein polypeptide comprising SEQ ID NO:3, comprising (a) providing a modified C-intein polypeptide comprising SEQ ID NO:4, wherein the functional amino acid side-chain of amino acid residue Asp 17 or at least one backbone amide group of amino acid residue selected from the group consisting of Gly19 and Gly31 of the modified C-intein polypeptide is protected using a 2-nitrobenzyl-based protecting group; and immobilizing the modified C-intein polypeptide to the surface; and (b) using UV-light to remove the 2-nitrobenzyl-based protecting group in order to activate the immobilized C-intein polypeptide thereby producing said surface.