Patent ID: 7547548

Claim:
A method for eliciting direct flower bud production in vitro on a soybean cotyledon, the method comprising 1) germinating at least one seed on a medium comprising MS and B 5 vitamins in amounts effective to cause formation of the soybean cotyledon, 2) splitting the soybean cotyledon, 3) transferring the split cotyledon to a cotyledon-flowering medium comprising: i) benzlamino purine (BAP) present at a concentration of about 0.75 mg/L to about 1.5 mg/L, and ii) 1-phenyl-3-(1,2,3-thidiazol-5-yl)urea (TDZ) present at a concentration of about 1.5 mg/L to about 2.5 mg/L, wherein the BAP and the TDZ are present in concentrations whereby no intervening shoot is formed on the cotyledon; 4) allowing the split cotyledon to develop at least one flower bud directly on the cotyledon, and 5) selecting for an in vitro flower formed directly on the split cotyledon.