Patent ID: 6927027

Claim:
A method of creating a nucleic acid multiplex, said method comprising the steps of: 1) creating a mixture comprising water, a Watson-Crick duplex, a sufficient number of single-stranded mixed base sequence molecules to form the multiplex including the Watson-Crick duplex, and an accelerator agent that increases a rate or amount of multiplex formation, said multiplex being a triplex or quadruplex; and 2) incubating said mixture to allow the multiplex to form, each strand of said multiplex related to all other strands of the multiplex by adherence to Watson-Crick base-pairing rules or homologous binding base-pairing rules; provided that, within the multiplex, the Watson-Crick duplex added in step (1) is heteropolymeric with a G-C content between 10% and 90% and a combined frequency therein of purine-pyrimidine dimers and pyrimidine-purine dimers exceeds 25%.