Patent ID: 7655404

Claim:
A method for the detection and/or quantification of at least one target nucleic acid or target polypeptide in a sample of nucleic acids or polypeptides comprising the steps of: (a) providing a sample comprising nucleic acids or polypeptides; (b) labeling the nucleic acids or polypeptides with a ligand conjugate, the ligand conjugate comprising a first element binding to the nucleic acids or polypeptides and a second element which is a capture ligand; (c) contacting the nucleic acids-ligand conjugate or polypeptides-ligand conjugate with at least one capture probe, the capture probe hybridizing with or binding to at least one target nucleic acid or target polypeptide; (d) adding i) an oxidoreductase enzyme, wherein the oxidoreductase enzyme is recognized by the capture ligand, or ii) a complex comprising an oxidoreductase enzyme bound to a capture receptor, the capture receptor capable of binding to the capture ligand; (e) adding a redox polymer, the redox polymer binding to the oxidoreductase enzyme, thereby resulting in the transfer of electrons from the enzyme via the redox polymer to an electrode surface; and (f) detecting and/or quantifying the presence of the target nucleic acid(s) or target polypeptide(s), wherein the first element binding to the nucleic acids or to the polypeptides is cisplatin, platinum-linked Cyanine 3, platinum-linked Cyanine 5, and the capture ligand is biotin, digoxigenin, an antibody or antigen binding to the capture receptor, an antibody binding to the oxidoreductase enzyme, an aptamer, a protein and/or a protein receptor.