Patent ID: 7678543

Claim:
A method for amplifying genomic DNA comprising the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45° to 80° C. and producing a genomic DNA-dispersed agarose solution thereby forming a dispersion of 0.002 to 1 copies/5 microliter of spatially separated single-stranded genomic DNA, then (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing the pH of the gel; and then (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.