Patent ID: 8541191

Claim:
A method of producing a diacylglyceride, the method comprising the steps of: (a) providing an oil composition comprising a quantity of triacylglyceride, (b) providing a polypeptide having a palmitase activity, said polypeptide being selected from the group consisting of isolated, synthetic and recombinant polypeptides having a palmitase activity and said polypeptide either i. being encoded by a nucleic acid comprising a nucleic acid sequence having at least 85% sequence identity to full length SEQ ID NO:1, and wherein the nucleic acid encodes at least one polypeptide having palmitase activity and the polypeptide having at least one amino acid residue change recited below in Table A, or ii. having at least 85% sequence identity to full length SEQ ID NO:2, and having at least one amino acid residue change recited in Table A, Table A Amino Original New Acid Amino Amino Residue Acid Acid 27 G Q 45 G L 54 S H 61 D A E 62 V A G 72 R E K S T 74 F I 83 V M 96 A I 98 G L 116 E A F H L Q Q R, H140R R S S T V 117 L R, I161L 120 K L 133 S A 147 I F 151 I A G H 160 P T 172 R Q 212 S R 213 K T 214 T V N R Y 215 G A H 216 A V C L or iii. having at least 85% sequence identity to full length of SEQ ID NO:2 and comprising at least one amino acid residue modification D61A; D61E; R72E; R72K; E116A; E116Q; E116R; E116T; E116V; S133A; I151G; I151A; V163R; or D164R (c) contacting said oil composition of step (a) with said polypeptide of step (b) under conditions sufficient for the polypeptide to hydrolyze a palmitase residue on the triacylglyceride to form a diacylglyceride.