Patent ID: 7220728

Claim:
A method of inducing in an individual an immune response against an antigen wherein said immune response includes both a humoral immune response that includes a mucosal immune response and a cellular immune response that includes antigen specific cytotoxic T lymphocytes; the method comprising the step of administering by topical or lavage administration to mucosal tissue of said individual, said mucosal tissue selected from the group consisting of rectal, vaginal, urethral, sublingual and buccal, a nucleic acid molecule that is free of an infectious-agent and comprises a nucleotide sequence that encodes said antigen operably linked to regulatory sequences in an amount effective to induce an immune response against said antigen wherein said immune response includes both a humoral immune response that includes a mucosal immune response and a cellular immune response; wherein said nucleic acid molecule is administered to said individual in a composition that further comprises bupivacaine.