Patent ID: 8192942

Claim:
A method for separating a target material (T) based on an aqueous two phase extraction system comprising the following steps: a) preparing a B-P conjugate by conjugating a partition enhancing material (P) to an affinity molecule (B), wherein the affinity molecule is an antibody that specifically binds the target material (T) thereby modifying the partition coefficient of B in order to locate the B-P conjugate in the opposite phase from where the target material is located; b) loading the B-P conjugate prepared in step a) into an aqueous two-phase extraction system that contains the target material (T), wherein the aqueous solution two-phase extraction system comprises a salt and a water-soluble polymer at a certain concentration, thereby generating a T-B-P conjugate by binding B-P conjugate to the target material (T), which is distributed mostly to upper water-soluble polymer phase; and c) isolating the upper water-soluble polymer phase to separate the T-B-P conjugate thereby separating the target material, wherein the partition enhancing material is HRP (horseradish peroxidase); and the water-soluble polymer is selected from the group consisting of PEG (polyethylene glycol) and dextran.