Patent ID: 8497069

Claim:
A method for amplifying a DNA template, comprising contacting a DNA with a strand-displacing, processive DNA polymerase and a set of random or partially random primers, under conditions that are effective for promoting DNA strand displacement, at an isothermal temperature, wherein the amplification is performed with a primer set in which the primers cannot base pair their 3′ nucleotides with other copies of the primers, wherein if the 3′ terminal nucleotide of the primer set is A, the nucleotide T is not present at any position in the primer set; if the 3′ terminal nucleotide of the primer set is T, the nucleotide A is not present at any position in the primer set; if the 3′ terminal nucleotide of the primer set is C, the nucleotide G is not present at any position in the primer set; and if the 3′ terminal nucleotide of the primer set is G, the nucleotide C is not present at any position in the primer set; and, further wherein the random or partially random primers are resistant to exonuclease.