Patent ID: 7967966

Claim:
A method for performing discontinuous gel electrophoresis, comprising: a) selecting a discontinuous electrophoresis system from a pair of discontinuous electrophoresis systems, wherein the electrophoresis system is selected based on a molecular weight of a protein and the separation characteristics of the electrophoresis system, wherein each electrophoresis system of the pair of electrophoresis systems separates proteins of different molecular weights, and wherein the pair of electrophoresis systems comprises: i) an electrophoresis separating gel comprising acrylamide and a gel buffer having a pH between 6 and 8, wherein the gel buffer comprises a primary organic amine or a substituted amine with a pK a near neutrality; and ii) two separate running buffers each comprising a different zwitterionic compound, and b) performing electrophoresis to resolve the protein in a sample using the selected electrophoresis system.