Patent ID: 6914236

Claim:
A scanning microscope comprising: at least one light source for generating an excitation light beam of a first wavelength and an emission light beam of a second wavelength, microscope optics for focusing the excitation light beam onto a first focal region in a first plane of a sample and for focusing the emission light beam onto a second focal region in a second plane of the sample, whereby the excitation light beam optically excites the sample in the first focal region and the emission light beam generates stimulated emission in the second focal region, and whereby the first and second focal regions are overlapping at least partially, said light source and said microscope optics defining an illumination beam path means for scanning the excitation light beam and the emission light beam onto a sample, components for guiding and shaping being arranged in the illumination beam path, whereby optical properties of the components and of the microscope optics are matched to one another such that optical aberrations are corrected in such a way that the focal regions remain static relative to one another irrespective of the scanning movement.