Patent ID: 8628932

Claim:
A method of screening for antibodies that specifically bind a native human leutkocyte antigen (HLA) antigen, comprising the steps of: obtaining a serum sample from a human subject, providing a first solid phase substrate having immobilized or attached thereon said HLA antigen which has been denatured, binding antibodies that specifically bind said denatured HLA antigen in said serum sample to said first solid-phase substrate to form an antibody/antigen complex, separating the antibody/antigen complex from the serum sample to generate a serum sample essentially free of antibodies that specifically bind said denatured HLA antigen, contacting the serum sample that is essentially free of antibodies that specifically bind said denatured HLA antigen with a second solid-phase substrate having native HLA antigen immobilized or attached thereto, and detecting binding of an antibody in said serum sample that is essentially free of antibodies that specifically bind denatured HLA antigen to the HLA antigen immobilized to the second solid-phase substrate in order to identify the potential presence of antibodies which are specific for native HLA antigen.