Patent ID: 8093068

Claim:
A method for selecting human antibody frameworks for use in making a human-adapted antibody comprising the steps of: a. obtaining a peptide sequence for a variable region of a non-human antibody; b. delineating the peptide sequences of the complementarity determining regions (CDRs) and framework regions of the non-human variable region; c. constructing a library of peptide sequences for human antibody framework 1, 2, 3 and 4 regions encoded by human germline genes; d. selecting a subset of member human peptide sequences from the library having an identical length to the framework regions of the non-human mature antibody; e. comparing the frame region sequence similarity of the selected human peptide sequences to the peptide sequences of the non-human framework regions; f. comparing the CDR1 and CDR2 length compatibility between the selected human peptide sequences and the peptide sequences of the non-human framework regions; g. selecting a second subset of the selected human peptide sequences wherein the peptide sequences selected have a frame region similarity of greater than an identity_threshold value and the sum of the length difference of CDR1 and CDR2 is smaller than or equal to a CDR12_length_compatibility_threshold value; and h. selecting representative frameworks from the second subset of step g) based upon a library_size value and a framework_region_redundancy value.