Patent ID: 8137954

Claim:
A method for producing an erythropoietin composition comprising the steps of: constructing a Pichia pastoris host cell to attach pre-selected N-linked glycans to proteins, said pre-selected N-linked glycans comprising greater than 25 mole percent of an N-linked glycan having the structure N-acetylglucosamine 2 Mannose 3 N-acetylglucosamine 2 Galactose 2 N-acetylneuraminic acid 2 (GlcNAc 2 Man 3 GlcNAc 2 Gal 2 NANA 2 ), and said host cell expressing a sequence encoding erythropoietin operably linked to expression control sequences recognizable by said host cell; culturing said transformed cell under conditions amenable to the expression of erythropoietin; and isolating said erythropoietin.