Patent ID: 7772005

Claim:
A method of establishing an equine artificial insemination sample for equine artificial insemination comprising the steps of: a. obtaining equine sperm cells from a male of a species of equine mammal; b. staining said equine sperm cells to allow differentiation based upon a sex characteristic; c. establishing a cell source which introduces said equine sperm cells which have been stained into a sheath fluid; d. forming droplets in said sheath fluid; e. entraining said equine sperm cells which have been stained in said droplets; f. differentiating between said equine sperm cells entrained in said droplets based upon said sex characteristic; g. separating said droplets based upon said sex characteristic of said equine sperm cells entrained at a rate of at least nine hundred live equine sperm cells per second; h. establishing a skim milk solution into which said droplets separated based upon said sex characteristic of said equine sperm cells entrained are collected; i. collecting live equine sperm cells separated based upon said sex characteristic in said skim milk solution at a rate of at least nine hundred live equine sperm cells per second; and j. establishing an equine artificial insemination sample containing said live equine sperm cells separated based upon said sex characteristic which are capable of fertilizing at least one egg within a female of said species of equine mammal.