Patent ID: 7781219

Claim:
A method for identifying a subject at risk of having cardiovascular disease, comprising: determining levels of one or more oxidized apolipoprotein A-1 (apoA-1)-related biomolecules in a biological sample of blood, serum, or plasma from the subject, wherein the one or more oxidized apoA-1-related biomolecules are oxidized apoA-1, oxidized apoA-1 peptide fragments derived from an oxidized apoA-1, or a combination thereof; wherein said oxidized apoA-1 fragments are at least five amino acids in length and comprise an oxidized amino acid; wherein the oxidized apoA-1 and the oxidized apoA-1 peptide fragments are lipid-free or bound to a lipid; wherein the oxidized apoA-1 and the oxidized apoA-1 from which the oxidized apoA-1 peptide fragments are derived have reduced cholesterol efflux stimulating activity as compared to un-oxidized apoA-1; and wherein elevated levels of the one or more oxidized apoA-1-related biomolecules in the biological sample as compared to a control value or an internal standard indicates that the subject is at risk of having cardiovascular disease.