Patent ID: 8252590

Claim:
A cell culture medium for the culture of mammalian cells, comprising basal culture media for mammalian cell culture and supernatant of fraction I of human plasma fractionation or supernatant of fraction II+III of human plasma fractionation, the supernatant of fraction 1 being prepared by precipitation with ethanol by adding ethanol to plasma up to a concentration of approximately 8% (volume/volume) and reducing temperature to approximately −2° C., separation of the supernatant, freezing or drying of the supernatant, and subsequent addition of the resulting supernatant to the basal cell culture, or the supernatant of fraction II+III being prepared by precipitation with ethanol by adding ethanol to a concentration of about 20% to about 25% (volume/volume) and reducing temperature to approximately −5° C., separation of the supernatant, freezing or drying, and subsequent addition of the resulting supernatant to the basal cell culture, wherein these supernatants have an albumin content between 60% and 78% and a gammaglobulins content between 1.5% to 12.5%.