Patent ID: 8691511

Claim:
A method for testing whether a compound increases the amount or activity of a protein encoded by an mRNA that contains a premature stop codon, comprising: (a) contacting in vitro a composition comprising a cell extract or cell lysate and the mRNA with an effective amount of a compound of the formula: or a pharmaceutically acceptable salt, stereoisomer or mixture of stereoisomers thereof, wherein: Z is substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted heterocycle, substituted or unsubstituted arylalkyl, or aryloxyalkyl; and X 1 ,X 2 , X 3 and X 4 are independently H or OH, and at least one of X 1 , X 2 , X 3 or X 4 is OH; and (b) detecting the amount or activity of the protein, wherein a compound that increases the amount or activity of the protein is detected if there is an increase in the amount or activity of the protein in the presence of the compound relative to the absence of the compound or the presence of a control.