Patent ID: 8551344

Claim:
A method of separating a clotting component from a whole blood sample in a container having a mixing assembly and a member, comprising: collecting a whole blood sample; introducing the whole blood sample into a single chamber of the container; manipulating the mixing assembly to mix the whole blood sample with the member to at least one of activate a portion of the whole blood sample or to withdraw a selected volume of water from the whole blood sample, wherein the mixing assembly includes a central member with radially extending agitation members; moving a piston within the single chamber of the container to compress the mixture of the whole blood sample and the member towards a first end of the container, wherein the compression with the piston at least assists in moving a selected component of the whole blood sample between a proximal surface of the piston and a second end of the container away from the first end; and withdrawing a volume of a fluid including concentrated clotting components from the single chamber of the container after mixing the whole blood sample with the member.