Patent ID: 7148334

Claim:
A gel electrophoresis sample composition that is resistant to protein degradation, comprising protein that is susceptible to degradation by protease, with protease being present in the sample composition, and with the sample composition containing small heat shock protein (sHSP) added to said protein susceptible to degradation by protease, in an amount in a range of from 0.1 to 50 parts, relative to 100 parts by weight of total protein in said sample composition, wherein said added sHSP includes sHSP selected from the group consisting of: IbpA (inclusion body-associated protein A) derived from Agrobacterium tumefaciens; sHSPs derived from Arabidopsis thaliana; HspB (heat shock protein B), HspH (heat shock protein H), HspC (heat shock protein C) and HspF (heat shock protein F) derived from Bradyrbizobium japonicum; IbpA derived from Brucella suis; sHPs derived from Buchnera aphidicola; IbpA derived from Buchnera aphidicola str. APS ( Acyrthosiphon pisum ); sHSPs derived from Citrus tristeza virus; IbpA and IbpB (inclusion body-associated protein B) derived from Escherichia coli; IbpB derived from Helicobacter pylori; Hsp 27 and α,β-crystallin derived from Human; Hsp 16.5 derived from Methanococcus jannaschii; IbpA derived from Methanopyrus kandleri; Hsp 25 derived from Murine; sHSPs derived from Mycobacterium leprae; Hsp 16.3 derived from Mycobacterium tuberculosis; IbpB derived from Pirellula sp.; Hspl 8.1 derived from Pisum sativum (pea); sHSPs derived from Plasmodium falciparum; IbpA derived from Pseudomonas aeruginosa; IbpA derived from Pseudomonas putida; Hsp 26 derived from Saccharomyces cerevisiae; IbpA and IbpB derived from Salmonella enterica; IbpA and IbpB derived from Salmonella typhimurium; IbpA derived from Shewanella oneidensis; IbpA and IbpB derived from Shigella flexneri; IbpA derived from Sinorhizobium meliloti; lbpA derived from Streptococcus pyogenes; sHSPs derived from Streptomyces coelicolor; sHSPs derived from Sulfolobus solfataricus; Hspl 6 derived from Synechococcus vulcanus; IbpA derived from Thermoanaerobacter tengcongensis; IbpA derived from Thermoplasma acidophilum ; and sHSPs IbpA and IbpB derived from Yersinia pestis.