Patent ID: 8287910

Claim:
A micelle having a polynucleotide encapsulated therein, comprising a multiblock copolymer of formula III-a: wherein: n is 50-2500; x 1 is 0 to 250; x 2 is 0 to 250; z 1 is 0 to 250, provided that x 1 and z 1 are not simultaneously zero; z 2 is 0 to 250, provided that z 1 and z 2 are not simultaneously zero; R x is a natural or unnatural amino acid side-chain group that is hydrophobic; R z is -L-R 4 wherein: L is a bivalent, straight or branched, C 4-20 alkylene chain wherein at least one methylene unit of -L- is replaced by â€”C(O)NRâ€” and 1-10 additional methylene units are independently replaced by â€”N(R)â€” or -Het-; Het is a bivalent 5-6 membered saturated, partially unsaturated, or aromatic ring having at least one nitrogen and 0-3 additional heteroatoms independently selected from nitrogen, oxygen, or sulfur; R 4 is R or N(R) 2 ; each R is hydrogen or C 1-6 aliphatic, or two R on the same nitrogen are taken together with the nitrogen to form a 5-6 membered heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R 1 is â€”Z(CH 2 CH 2 Y) p (CH 2 ) t R 3 , wherein: Z is a valence bond, a bivalent triazolyl moiety, â€”Oâ€”, â€”Sâ€”, â€”Câ‰¡Câ€”, or â€”CH 2 â€”; each Y is independently â€”Oâ€” or â€”Sâ€”; p is 0-10; t is 0-10; and R 3 is â€”N 3 , â€”CN, a mono-protected amine, a di-protected amine, a protected aldehyde, a protected hydroxyl, a protected carboxylic acid, a protected thiol, a 9-30 membered crown ether, or an optionally substituted group selected from aliphatic, a 5-8 membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, an 8-10 membered saturated, partially unsaturated, or aryl bicyclic ring having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a detectable moiety or a targeting group; each Q is independently a valence bond or a bivalent, saturated or unsaturated, straight or branched C 1-12 alkylene chain, wherein 0-6 methylene units of Q are independently replaced by -Cy-, â€”Oâ€”, â€”NHâ€”, â€”Sâ€”, â€”OC(O)â€”, â€”C(O)Oâ€”, â€”C(O)â€”, â€”SOâ€”, â€”SO 2 â€”, â€”NHSO 2 â€”, â€”SO 2 NHâ€”, â€”NHC(O)â€”, â€”C(O)NHâ€”, â€”OC(O)NHâ€”, or â€”NHC(O)Oâ€”, wherein: -Cy- is an optionally substituted 5-8 membered bivalent, saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an optionally substituted 8-10 membered bivalent saturated, partially unsaturated, or aryl bicyclic ring having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.