Patent ID: 8452359

Claim:
A method for building an algorithm for converting spectral information obtained from body tissue into the concentration of an analyte in at least one body fluid in the body tissue, the method comprising the acts of: modulating the concentration of the analyte in at least one body fluid in the body tissue to a plurality of predetermined levels within a range of predetermined analyte concentration levels; directing infrared light onto the body tissue of at least one test subject being analyzed during an analyte clamping test; collecting spectral information from the body tissue; collecting data other than spectral information from the body tissue of the at least one test subject during the modulating of the concentration of the analyte in at least one body fluid in the body tissue; modeling spectral information corresponding to analyte concentration levels outside the range of analyte concentration levels; and filtering the collected and modeled spectral information to remove non-analyte spectral information using one or more chemometric techniques selected from the group consisting of wavelets analysis, orthogonal signal correction, partial least squares regression, principal components analysis, and genetic algorithms.