Patent ID: 7371922

Claim:
A method for producing a porcine mammal with a nuclear genome of interest, said method comprising the steps of: (a) providing a porcine embryonic stem cell comprising a nucleus having a genome of interest; (b) providing a porcine oocyte that is a matured and activated oocyte, culturing a porcine oocyte so that it is a matured and activated oocyte, or producing a porcine zygote in vivo or in vitro; (c) enucleating the matured and activated oocyte or enucleating the zygote to step (b) to produce an enucleated recipient cell; (d) transferring the nucleus of the embryonic stem cell of step (a) to the enucleated recipient cell of step (c) to produce a nuclear transfer embryo; (e) culturing the nuclear transfer embryo of step (d) to produce an embryo of a 4-cell, 8-cell, 16-cell, compact morula or blastocyst stage of development; and (f) implanting the nuclear transfer embryo of step (d or e) into a surrogate porcine mother mammal, whereby a porcine mammal having a nuclear genome of interest is produced.