Patent ID: 8062886

Claim:
A method for producing an antibody comprising culturing an isolated host cell comprising a recombined amplifiable vector under conditions favorable to expression of cassettes in said vector; which vector is the product of a method comprising: (a) introducing one or more expression cassettes encoding an immunoglobulin light chain or immunoglobulin heavy chain of said antibody into the multiple cloning site of a first universal transfer vector comprising a first multiple cloning site which comprises nucleotides 620-766 of the nucleotide sequence set forth in SEQ ID NO: 2; wherein said multiple cloning site is in said orientation or in a reverse orientation; (b) introducing one or more expression cassettes encoding an immunoglobulin light chain or heavy chain of said antibody, different from the cassettes introduced into said first universal transfer vector, into a second universal transfer vector comprising a second multiple cloning site which comprises nucleotides 620-772 of the nucleotide sequence set forth in SEQ ID NO: 1; wherein said multiple cloning site is in said orientation or in a reverse orientation; and (c) introducing said cassettes from steps (a) and (b) into an amplifiable vector comprising a third multiple cloning site, comprising nucleotides 5037-5183 of the nucleotide sequence set forth in SEQ ID NO: 3; wherein said multiple cloning site is in said orientation or in a reverse orientation; and wherein the cassettes from steps (a) and (b) encode an immunoglobulin light chain and an immunoglobulin heavy chain, of said antibody.