Patent ID: 7645866

Claim:
A method for producing a modified polynucleotide sequence comprising: a) annealing at least one primer to a template polynucleotide sequence; b) extending said primer in the presence one or more nucleoside triphosphates, wherein at least one of the nucleoside triphosphates is a modified nucleoside triphosphate comprising a general structure [I]: wherein R 1 is hydrogen, a substituted or non-substituted: alkyl, akenyl, alkynyl or aryl group, or R 2 ; wherein R 2 is —SH, or —SR 3 ; and wherein R 3 is a substituted or non-substituted: alkyl, akenyl, alkynyl or aryl group; thereby producing a modified polynucleotide sequence comprising at least one modified nucleotide, wherein said modified polynucleotide sequence comprises a general structure [II]: wherein the general structure [II] comprises a 3′ phosphorothiolate linkage.