Patent ID: 7939318

Claim:
A method for making a virus-like particle (VLP) containing multiple, different composition peptides or proteins displayed by a process comprising the steps of: a) disassembling separate VLP populations, each displaying a distinct peptide or protein via genetic fusion; b) disassembling a separate VLP population that has a surface residue for chemical conjugation, provided by genetic fusion; c) forming encapsidation intermediate populations such that: i) each displays a distinct peptide or protein and ii) each displays a surface residue for chemical conjugation; d) effecting chemical conjugation of unique peptide, protein or nucleic acid moieties to separate populations of the encapsidation intermediate displaying surface residue for chemical conjugation; e) mixing encapsidation intermediates from different populations displaying peptides or proteins by genetic fusion or displaying peptides, proteins or nucleic acids by chemical conjugation; f) forming intact VLP surrounding a nucleic acid core that is composed of different encapsidation intermediates such that the VLP displays more than one moiety, be it peptide, protein or nucleic acid, or some combination of these moieties.