Patent ID: 8809513

Claim:
A composition comprising a pool of oligonucleotides that do not hybridize to one or more selected sequences prepared by a process comprising: selecting at least one exclusion sequence to which hybridization of a pool of oligonucleotides of length n is not desired; identifying a set of all possible sequences of length n; identifying a set of all sequences of length n contained in the exclusion sequence; excluding from the pool of oligonucleotides those oligonucleotides of length n that are identical to a sequence of length n contained in the exclusion sequence; excluding from the pool of oligonucleotides those oligonucleotides in which the only mismatch is a GU at the 5′ end of the sequence of length n; and wherein a pool of oligonucleotides of length n that do not hybridize to the exclusion sequence are identified.