Patent ID: 6942984

Claim:
A method for identifying a candidate modulator of CARD-12, the method comprising: a) contacting a polypeptide selected from the group consisting of: i) a polypeptide comprising an amino acid sequence at least 90% identical to amino acid residues 161-323 of SEQ ID NO:2; ii) a polypeptide comprising an amino acid sequence at least 90% identical to amino acid residues 169-456 of SEQ ID NO:2 of CARD-12; iii) a polypeptide with a length of at least 25 amino acids, comprising at least one NACHT NTPase domain (amino acid residues 169-186, 196-220, 229-253, 261-282, 330-351, 414-430, or 438-457 of SEQ ID NO:2) of CARD-12; and iv) a polypeptide with a length of at least 25 amino acids, comprising the P-loop (amino acid residues 169-179 of SEQ ID NO:2) of CARD-12, with a test compound in the presence of a nucleotide that binds to the polypeptide in the absence of the test compound; b) measuring the binding of the nucleotide to the polypeptide in the presence of the test compound; and c) identifying the test compound as a candidate modulator of CARD-12 if the test compound increases or decreases the binding of the nucleotide to the polypeptide.