Patent ID: 7485770

Claim:
A method of introgressing one or more alleles into a soybean plant comprising (A) crossing at least one SCN resistant soybean plant with at least one SCN sensitive soybean plant in order to form a segregating population, (B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains an rhg1 SCN resistant allele and an Rhg4 SCN resistant allele, wherein said rhg1 SCN resistant allele is an allele having a deletion of 19 nucleotides corresponding to SEQ ID NO: 2 and encompassing position 48881, and said Rhg4 SCN resistant allele is an allele having one or more polymorphisms located at a position in SEQ ID NO: 4 selected from the group consisting of 111933, 112065, 112101, and 112461, and (C) selecting, if present, one or more soybean plants of said segregating population having said deletion and comprising said one or more polymorphisms located at a position in SEQ ID NO: 4.