Patent ID: 7696406

Claim:
A method of producing a heterologous polypeptide, the method comprising: a) providing a transgenic plant or a transgenic plant cell comprising a recombinant DNA molecule comprising, in the 5′ to 3′ direction, i) a plant promoter; ii) a sequence complementary to a coding sequence for a heterologous polypeptide; iii) a sequence complementary to a plant virus internal ribosome entry site; and iv) a 3′ UTR sequence having a sequence encoding a viral RNA replication initiation site; b) growing the transgenic plant or transgenic plant cell; c) producing an RNA transcript of the DNA sequence in the transgenic plant or the transgenic plant cell, the RNA transcript being a complementary RNA copy of the DNA sequence; d) infecting the transgenic plant or the transgenic plant cell with an RNA nucleic acid encoding an RNA-dependent RNA polymerase operable to recognize the viral RNA replication initiation site and convert the RNA transcript produced by the transgenic plant or the transgenic plant cell to a translatable mRNA, the mRNA having a RNA sequence comprising, in the 5′ to 3′ direction: i) a sequence complementary to the 3′ UTR sequence; ii) a coding sequence of the plant virus internal ribosome entry site; and iii) a coding sequence of the heterologous polypeptide; and e) translating the translatable mRNA in the transgenic plant or the transgenic plant cell to form the heterologous polypeptide.