Patent ID: 6893831

Claim:
An immunoassay method for measuring PIVKA-II in a serum or plasma test sample, comprising the steps of: (a) contacting the serum or plasma test sample with a first immunoassay reagent comprising anti-PIVKA-II antibodies and antibodies which specifically bind to a human fibrin or a human fibrinogen; (b) contacting the resultant sample with a second immunoassay reagent comprising labeled antibodies which bind PIVKA-II, wherein the labeled antibodies are at least one antibody selected from the group consisting of anti-human prothrombin antibody, anti-F 1 antibody to the F 1 prothrombin fragment, anti-F 2 antibody to the F 2 prothrombin fragment and anti-(F 1 +F 2 ) antibody to the F 1 and F 2 prothrombin fragments; and (c) measuring the labeled antibodies, thereby measuring PIVKA-II in the serum or plasma test sample.