Patent ID: 8420026

Claim:
A microfluidic device comprising: a body of revolution; and at least one microfluidic structure disposed in the body of revolution, wherein the microfluidic structure comprises: a plurality of chambers; a plurality of channels which connect the chambers; and a plurality of valves which are disposed in the channels to control fluid flow, and wherein the microfluidic structure transmits fluid using centrifugal force due to rotational movement of the body of revolution, wherein the chambers comprise: a sample chamber which contains a material sample; a buffer chamber which contains a buffer solution; a mixing chamber which contains micro-particles, and which is connected with the sample chamber and the buffer chamber so that the material sample, and the buffer solution flow into the mixing chamber, wherein the material sample and the buffer solution flow into the mixing chamber by control of valves disposed in corresponding channels, wherein the mixing chamber comprises an outlet having a valve at a most distant position from a center of the body of revolution, and wherein in the mixing chamber, the micro-particles capture a target material from the material sample, and the micro-particles are washed by the buffer solution; and a waste chamber which is connected with a part, which is closer to the center of the body of revolution than the outlet of the mixing chamber, through a waste channel, and which contains fluid discharged from the mixing chamber by control of a valve disposed in the waste channel; a cytolysis chamber which is connected with the outlet of the mixing chamber through a cytolysis channel, and which contains fluid comprising the micro-particles discharged from the outlet of the mixing chamber, and an external energy source configured to irradiate electromagnetic waves, wherein in the cytolysis chamber, a micro element is separated from the target material by irradiation of the electromagnetic waves from the external energy source onto the micro-particles.