Patent ID: 7060470

Claim:
A method of producing isoflavone-β-D-glucan, which comprises the steps of: a) culturing Agaricus blazei mycelia in a liquid medium containing isoflavone to produce an undigested isoflavone-β-D-glucan; b) separating the undigested isoflavone-β-D-glucan from the liquid culture of Agaricus blazei mycelia; c) separating an autolysis enzyme from a separate liquid culture of Agaricus blazei mycelia; d) adding the autolysis enzyme to the undigested isoflavone-β-D-glucan to produce a digested isoflavone-β-D-glucan having a molecular weight of 30,000 or less; and e) separating and purifying the digested isoflavone-β-D-glucan.