Patent ID: 8637679

Claim:
A process for the isolation of compound of general formula 1 wherein R 1 ═H mahanimbine (1a) or R 1 ═OH mahanine (1b); from Murraya koeniigii , said process comprising the steps of: i. extracting leaves of Murraya koeniigii with methanol followed by concentrating to obtain residue; ii. dissolving the residue as obtained in step (i) in a solvent followed by adding 8 to 10% acid; iii. separating the acid soluble part from the mixture as obtained in step (ii) and making the remaining solution alkaline to obtain a precipitate; iv. dissolving the precipitate as obtained in step (iii) in ethyl acetate followed by evaporating to obtain an alkaloid; and v. subjecting the alkaloid as obtained in step (iv) to repeated chromatography on silica gel using petrol-chloroform solvent as eluent followed by crystallization on petrol to obtain the compound of general formula 1.