Patent ID: 6927042

Claim:
A method for synthesis of a glycoprotein, the method comprising: incorporating, in a cell, an unnatural amino acid that comprises a first reactive group into a protein; wherein the incorporating step comprises incorporating the unnatural amino acid into the protein with an orthogonal tRNA/orthogonal aminoacyl-tRNA synthetase (O-tRNA/O-RS) pair, wherein the O-tRNA recognizes a selector codon and incorporates the unnatural amino acid into the protein in response to the selector codon, and wherein the O-RS aminoacylates the O-tRNA with the unnatural amino acid, and contacting the protein with a saccharide moiety that comprises a second reactive group, wherein the first reactive group reacts with the second reactive group to attach the saccharide moiety to the unnatural amino acid, thereby producing the glycoprotein.