Patent ID: 7202042

Claim:
A method for determining the ratio of oxidized cardiac troponin I to reduced cardiac troponin I in a patient sample, comprising: a) contacting the patient sample with i) a first antibody that specifically binds to both oxidized and reduced cardiac troponin I, wherein said oxidized and reduced cardiac troponin I bind to the first antibody in an amount proportional to their ratio in the sample, and ii) a second antibody that specifically binds to one of oxidized or reduced cardiac troponin I, whereby said first and second antibodies form a complex comprising one of said oxidized or reduced cardiac troponin I present in said sample, and wherein if said second antibody forms a complex comprising oxidized troponin I, said second antibody does not form a complex comprising reduced troponin I and wherein if said second antibody forms a complex comprising reduced troponin I, said second antibody does not form a complex comprising oxidized troponin I; and b) generating a signal corresponding to the amount of said complex formed and relating said signal to said ratio.