Patent ID: 8563321

Claim:
A method for enhancing the chemiluminescence of 1,2-dioxetane compounds, the method comprising: providing at least one alkyl bis-quaternary ammonium salt having the structure of General Formula I: wherein: R 1 is selected from at least one of the following: C 10-20 alkyl, C 10-20 alkenyl, C 10-20 alkynyl, and C 3-8 cycloalkylC 6-12 alkyl-; each n is an integer independently selected from 2 to 15; each m is an integer independently selected from 1 to 6; R 2 , R 3 , R 4 , R 5 , R 6 , and R 7 are each independently selected from at least one of the following: C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, and C 3-8 cycloalkyl; and X − is a negative ion; and mixing the alkyl bis-quaternary ammonium salt with a 1,2-dioxetane compound.