Patent ID: 7919243

Claim:
A method for the specific detection of Fusarium graminearum ( Gibberella zeae ) comprising: providing a sample containing a nucleic acid; contacting said sample with at least one forward primer and at least one reverse primer, wherein the at least one reverse primer hybridizes within the β-tubulin nucleic acid sequence of Fusarium graminearum ( Gibberella zeae ) and comprises the nucleic acid sequence 5′-R1TTTTCGTX1GX2AGT-3′ (SEQ ID NO: 1), wherein R1 comprises at least one nucleic acid residue of the β-tubulin nucleic acid sequence located upstream of the hybridization site of the nucleic acid sequence SEQ ID NO: 1 and subsequent to the nucleic acid sequence SEQ ID NO: 1, X1 is guanine, adenine or inosine, X2 is cytosine, adenine or inosine, and wherein the at least one forward primer hybridizes upstream of the hybridization site of a nucleic acid sequence complementary to the at least one reverse primer; and subjecting the sample contacted with the at least one forward primer and the at least one reverse primer to a nucleic acid amplification technique.