Patent ID: 6947144

Claim:
A fluorometry method for monitoring the transformation of plants, comprising the steps: projecting excitation light from a light source through a blue bandpass filter onto a plant sample at an angle of 45°, said blue bandpass filter passing light ranging, in wavelength, from 470 to 490 nm, said excitation light having a wavelength of around 488 rim with a peak at 480 nm; detecting light generated from the plant sample by use of a charge coupled device color video camera equipped with a zoom lens, which is positioned on the axis vertical to the plane of the sample, said light passing through a green bandpass filter which passes light ranging, in wavelength, from 500 to 550 nm before arriving at said zoom lens, so as to have a wavelength of around 509 nm, and photographing the image of the plant sample on the basis of the light generated from the plant sample; and processing the image in a computer to determine whether the plant sample is transgenic or not based on the in vivo expression of a heterologous green fluorescent protein.