Patent ID: 7883894

Claim:
A process for reversibly increasing the recombination rate between a first-DNA sequence and a second DNA sequence in vivo in a prokaryotic organism, wherein the process comprises: a) providing to the prokaryotic organism the first DNA sequence obtained from a first species or a first genus and the second DNA sequence obtained from a second species or a second genus, wherein the first and the second DNA sequences, are partially homologous with up to 30% mismatches capable of activating an enzymatic mismatch repair System (MRS) of the prokaryotic organism when the MRS is functional, b) subjecting the prokaryotic organism comprising the first and the second DNA sequences to 2-aminopurine in an amount and for a period of time sufficient to inactivate the MRS and thus increase the recombination rate, wherein the period of time is between 1 and 5 hours; and c) removing the 2-aminopurine from the prokaryotic organism to reverse the increased recombination rate.