Patent ID: 7745645

Claim:
At least one compound of formula I or formula II wherein R 1 and R 2 are the same or different and are independently selected from the group consisting of —H, —C 1 -C 18 -alkyl or -ω-sulfoalkyl; X and Y are the same or different and are independently selected from the group consisting of —O − , —OH, —SH, —NH—NH 2 , —F, —Cl, —Br, —I, —O-Su (succinimidyl/sulfosuccinimidyl), —O-STP (4-sulfo-2,3,5,6-tetrafluorophenyl), —O-TFP (2,3,5,6-tetrafluorophenyl), —O-benzotriazole, -benzotriazole, —NR—CO—CH 2 —I, —NR 2 , —NR-biomolecule, —NR-L-COO − , —NR-L-COOH, —NR-L-COO-Su, —NR-L-COO-STP, —NR-L-COO-TFP, —NR-L-CONR 2 , —NR-L-CO-biomolecule, —NR-L-CO—NH—NH 2 , —NR-L-OH, —NR-L-O-phosphoramidite, —NR-L-CHO, —NR-L-maleimid, or —NR-L-NH—CO—CH 2 —I; where R is equal to R 1 and R 2 and L is selected from the group consisting of a divalent linear (—(CH 2 ) o —, o=1 to 15), crossed, or cyclic alkane group that can be substituted by at least one atom selected from the group consisting of oxygen, substituted nitrogen, and/or sulfur, and the biomolecule is a protein, antibody, nucleotide, oligonucleotide, biotin, or hapten; Z is —O − or OH; U is —O − , —OH or NH-L-SO 2 Z; Kat is Li, Na, K, ammonium (mono-, di- or trialkyl) or another cation; An is F, Cl, Br, I, BF 4 , ClO 4 , CH 3 CO 2 , CF 3 CO 2 or another anion; m is an integer from 1-6 necessary to compensate the negative or positive charge from the dye moiety in formula I or formula II; and n is an integer from 0-12.