Patent ID: 8415148

Claim:
A recombinant vesicular stomatitis virus vector expression system comprising (a) a VSV comprising a polynucleotide encoding a T7 RNA polymerase or other bacteriophage RNA polymerase and the D1 subunit of vaccinia capping enzyme, wherein when the vector is used to infect a cell, the RNA polymerase and the D1 subunit of vaccinia capping enzyme are expressed in the cell; (b) a recombinant plasmid vector comprising a polynucleotide comprising the following elements operably linked 5′ to 3′: (i) a bacteriophage promoter corresponding to the bacteriophage RNA polymerase, and (ii) a heterologous gene, wherein when the recombinant plasmid vector is used to transfect said cell, a polypeptide is expressed from transcripts encoded by said heterologous gene in the cell.