Patent ID: 7211443

Claim:
A method for detecting or quantitating one or more polynucleotide sequences in a sample, said method comprising providing a device comprising a substrate defining a sample-distribution network including (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein at least two of said detection chambers each contain pre-loaded therein, at least one different, sequence-specific polynucleotide binding polymer for detecting or quantitating different polynucleotide sequences that may be present in such sample, to produce a detectable signal, applying the sample to the sample inlet and delivering the sample into the network by action of a force whereby the sample is delivered to the detection chambers, allowing the delivered sample to react with the at least one different, sequence-specific polynucleotide binding polymer in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when the one or more polynucleotide sequences are present in the sample, and measuring the detectable signal to detect or quantitate the one or more polynucleotide sequences in the sample.