Patent ID: 8435735

Claim:
A method of detecting a target in a sample, the method comprising: (i) Incubating a target or a sample comprising a target with one or more binding agents comprising peroxidase activity, wherein the one or more binding agents is/are capable of direct or indirect binding to the target and forming a complex comprising the target and the one or more binding agents, and wherein at least one of the one or more binding agents comprises peroxidase activity; (ii) incubating the complex of (i) in a water solution comprising: 1) 3,3′ diaminobenzidine; 2) a peroxide compound; and 3) a compound, wherein the compound is a conjugate molecule comprising: two or more moieties capable of serving as substrate for a peroxidase enzyme, one or more detectable labels, and a water soluble polymer; wherein said two or more moieties capable of serving as substrate for a peroxidase enzyme and said one or more detectable labels are linked to said polymer so that the distance separating any of the linked moieties from any of the linked labels is at least 30 consecutively interconnected atoms, and wherein the distance separating any two of the two or more linked moieties capable of serving as substrate for a peroxidase enzyme is less than 30 consecutively interconnected atoms; and thereby depositing said conjugate molecule; (iii) detecting the deposited conjugate molecule, and thereby detecting the target.