Patent ID: 7897360

Claim:
A lateral flow assay device for detecting the presence or quantity of an enzyme, or an inhibitor thereof, within a test sample, the lateral flow assay device comprising a chromatographic medium defining a first detection zone and a second detection zone, the chromatographic medium further defining an application area within which a molecular substrate is diffusibly immobilized, wherein said test sample contacts said molecular substrate within said application area, the molecular substrate being capable of undergoing a reaction catalyzed by the enzyme to form a product, the lateral flow assay device further defining a conjugate zone downstream of said application area, a first detectable substance being diffusibly immobilized within said conjugate zone, said first detectable substance directly or indirectly binding with said product to generate a first detection signal, the lateral flow assay comprising a second detectable substance that directly or indirectly binds with said molecular substrate to generate a second detection signal, wherein the presence or quantity of an enzyme, or an inhibitor thereof, is determinable from said first detection signal within said first detection zone and said second detection signal within said second detection zone.