Patent ID: 7910309

Claim:
An assay for detecting the presence of Xylella fastidiosa strain 9a5c in a citrus plant, an environmental sample or an insect, comprising: (a) extracting or releasing nucleic acid from a sample of the citrus plant, environmental sample or insect; (b) amplifying a XF0324 gene target nucleic acid using nucleic acid sequence based amplification (NASBA) with the amplification primers of SEQ ID NOS: 1 and 2, to generate a solution containing amplified single-stranded RNA amplification product complementary to the target nucleic acid, if present in the nucleic acid from the sample; and, (c) detecting the presence of the RNA amplification product using (i) a detectably-labeled detection oligonucleotide which comprises a sequence complementary to a first sequence of the RNA amplification product, and (ii) a capture oligonucleotide which comprises a sequence complementary to a second sequence of the RNA amplification product.