Patent ID: 7462487

Claim:
A composition of serum-free cell culture medium having the following components present at the indicated final concentrations: calcium chloride at 180 mg/L, potassium chloride at 298 mg/L, potassium nitrate at 0.0126 mg/L, magnesium sulfate at 68 mg/L, magnesium chloride at 37 mg/L, sodium chloride at 6200 mg/L, sodium bicarbonate at 1200 mg/L, sodium phosphate at 43 mg/L, sodium phosphate (dibasic) at 88 mg/L, sodium selenite at 0.0126 mg/L, ammonium metavanadate 3.51×10 −4 mg/L, molybdic acid at 3.72×10 −3 mg/L, cupric sulfate at 7.5×10 4 mg/L, ferrous sulfate at 0.25 mg/L, manganese sulfate at 4.53×10 −5 mg/L, zinc sulfate at 0.259 mg/L, fructose at 2000 mg/L, HEPES at 15 mM, putrescine at 0.048 mg/L, thioctic acid at 0.0618 mg/L, sodium pyruvate at 110.03 mg/L, linoleic acid at 0.025 mg/L, 1-alanine at 20.173 mg/L, 1-asparagine (freebase) at 17.5 mg/L, 1-asparagine at 122.01 mg/L, 1-aspartic acid at 24.99 mg/L, 1-cystine at 63.98 mg/L, 1-cysteine at 5.268 mg/L, glutamic acid at 56.9 mg/L, 1-glutamine at 600 mg/L, glycine at 23.25 mg/L, 1-histidine at 35.69 mg/L, 1-isoleucine at 74.68 mg/L, leucine at 77.43 mg/L, 1-lysine at 113.16 mg/L, 1-methionine at 22.34 mg/L, 1-phenylalanine at 47.69 mg/L, 1-proline at 38.36 mg/L, 1-seine at 32.55 mg/L, 1-threonine at 70.07 mg/L, 1-tryptophan at 11.81 mg/L, 1-tyrosine at 75.17 mg/L, 1-valine at 69.31 mg/L, biotin at 1.13 ×102 mg/L, D-Ca-pantothenate at 2.87 mg/L, choline chloride at 6.99 mg/L, folic acid at 3.2 mg/L, 1-inositol at 10.45 mg/L, niacinamide at 2.81 mg/L, pyriodxal at 2.8 mg/L, pyridoxine at 1.85×10 −2 mg/L, riboflavin at 0.291 mg/L, thiamine at 2.9 mg/L, vitamin B 12 at 4.99×10 −2 mg/L.