Patent ID: 8703487

Claim:
A composition, a product or an article of manufacture, or an isolate, a mixture or a culture of human cells, for producing human erythroid stem cells or human erythroid progenitor cells for in vivo applications, comprising: (1) in vitro-activated human bone marrow mesenchymal stem cells (BM-MSC, MSC); (2) human embryoid bodies (EBs) or human pluripotent stem cells; and, (3) a sufficient amount of a human erythropoietin to induce formation or differentiation to human erythroid stem cells or human erythroid progenitor cells, wherein the human BM-MSC/MSC cells are activated by a method comprising (a) providing: (i) a human bone marrow mesenchymal stem cell (BM-MSC) or a human Marrow Stromal Cell; or, (ii) a human bone marrow-derived cell that expresses or is positive for a the cell surface polypeptides CD105, CD166, CD29 and CD44, and does not express or is negative for the cell surface polypeptides CD14, CD34 and CD45; and (b) culturing the cell of (a) under hypoxic conditions for at least about 6 or more hours, or between 1 to 2 days.