Patent ID: 8221982

Claim:
A composition comprising: (a) one or more first nucleic acid molecules, wherein each first nucleic acid molecule is circular and comprises, in a 5′ to 3′ orientation, a first restriction site RA 0 , any DNA segment selected from the group D 0 , an annealable linker sequence LB 0 , and a second restriction site RB 0 ; (b) one or more intermediate nucleic acid molecules, wherein each intermediate nucleic acid molecule n is circular and comprises, in a 5′ to 3′ orientation, a first restriction site RA n , a first annealable linker sequence LA n , any DNA segment selected from the group D n , a second annealable linker sequence LB n , and a second restriction site RB n , and wherein n represents an integer from one to the number of intermediate nucleic acid molecules; and (c) one or more last nucleic acid molecules, wherein each last nucleic acid molecule is circular and comprises, in a 5′ to 3′ orientation, a first restriction site RA m , an annealable linker sequence LA m , any DNA segment selected from the group D m , a second restriction site RB m , wherein m represents an integer one greater than the number of intermediate nucleic acid molecules; whereupon cleavage of restriction sites RA 0 through RB m and denaturation of the resulting linear nucleic acid molecules, each annealable linker sequence LB (p-1) is capable of hybridizing to the complement of annealable linker sequence LA p , wherein n is an integer that varies from 1 to (m−1), wherein p represents an integer from 1 to m, and wherein each group D 0 , . . . D n , . . . D m independently consists of one or more DNA segments.