Patent ID: 7303877

Claim:
A method of producing a heterologous polypeptide, comprising: (a) cultivating a mutant of a parent Aspergillus niger strain in a medium suitable for the production of the heterologous polypeptide, wherein (i) the mutant strain comprises a first nucleotide sequence encoding the heterologous polypeptide and one or more second nucleotide sequences comprising a modification of glaA, asa, amyA, amyB, prtT, and oah, and (ii) the mutant strain is deficient in the production of glucoamylase, acid stable alpha-amylase, neutral alpha-amylase A, neutral alpha-amylase B, protease, and oxalic acid hydrolase compared to the parent Aspergillus niger strain when cultivated under identical conditions; and (b) recovering the heterologous polypeptide from the cultivation medium.