Patent ID: 7797112

Claim:
A method of identifying an inhibiting agent against FabK (enoyl-acyl carrier protein reductase), the method comprising: (a) generating on a computer a three-dimensional structure of a FabK-FMN (flavin mononucleotide) complex having the atomic coordinates of Table 2; (b) identifying amino acids of FabK forming an FMN binding site of FabK from the three-dimensional structure generated in step (a), wherein the amino acids of FabK forming the FMN binding site are amino acids 198-205, 208-215, 229, 261, 276, and 278-283 of SEQ ID NO:1; (c) generating a three-dimensional model of the FMN binding site of FabK, wherein the amino acids of the three-dimensional model have the atomic coordinates according to Table 2; (d) employing said three-dimensional model from step (c) to identify a potential inhibiting agent of FabK; (e) obtaining said potential inhibiting agent; and (f) contacting said potential inhibiting agent with FabK to determine the ability of said potential inhibiting agent to inhibit FabK activity, whereby inhibition of FabK activity identifies said inhibiting agent.