Patent ID: 7314714

Claim:
A method of amplifying a plurality of sequences of interest, comprising: (a) synthesizing a plurality of template oligonucleotides on a solid support, wherein full length template oligonucleotides are nuclease resistant, and wherein the template oligonucleotides are attached to the solid support by a cleavable portion and releasing the template oligonucleotides from the solid support by cleaving the cleavable portion; (b) digesting oligonucleotides that are not nuclease resistant with an exonuclease; (c) making the template oligonucleotides double stranded; (d) nicking one strand of the double stranded template oligonucleotides, thereby generating a 5′ and a 3′ portion of one strand, wherein the 3′ portion comprises a sequence of interest; and, (e) extending the 5′ portion with a DNA polymerase wherein the 3′ portion is released and a new copy of the 3′ portion is synthesized.