Patent ID: 8293521

Claim:
A nonvolatile liquid dispensing method comprising: providing a reaction vessel comprising at least a typing reagent reservoir reserving a typing reagent, a gene amplification reagent reservoir for reserving a gene amplification reagent containing a plurality of primers to bind to a plurality of polymorphic sites by sandwiching each site between the primers, an amplification reaction part that allows a gene amplification reaction for a mixture solution of the gene amplification reagent and a sample, a plurality of probe arrangement parts each individually holding a probe emitting fluorescence in correspondence with each of a plurality of polymorphic sites and a nonvolatile liquid reservoir for reserving the nonvolatile liquid having a lower specific gravity than a reaction solution, the amplification reaction part having two dispensing ports opened upwardly at both ends thereof; dispensing the nonvolatile liquid into the amplification reaction part before the gene amplification reaction in the condition that an amount more than a single dispensing amount of the nonvolatile liquid is aspirated and held in a nozzle; dispensing the nonvolatile liquid through one of the dispensing ports of the amplification reaction part after the gene amplification reaction to facilitate collection of a reaction-finished solution, the reaction-finished solution being pushed to the other dispensing port by the dispensing of the nonvolatile liquid; and dispensing the nonvolatile liquid into the probe arrangement parts in the condition that an amount more than a single dispensing amount of the nonvolatile liquid is aspirated and held in the nozzle.