Patent ID: 8067176

Claim:
A chemical reaction method for conducting a chemical reaction in a fluid of a droplet inside a reaction receptacle or on a surface of a reaction substrate, comprising the steps of: providing fluctuations of a magnetic field to the droplet including an aqueous solution having magnetic body particles with a hydrophilic surface, transmitting a physical force to the aqueous solution through the magnetic body particles, moving the droplet by the physical force, and conducting an operation necessary for a chemical reaction, wherein the reaction receptacle or the reaction substrate comprises a temperature variation area in which a temperature changes continuously, the droplet being moved to at least one spot inside the temperature variation area by the fluctuations of the magnetic field, and the chemical reaction is conducted by controlling temperature of the droplet, wherein the droplet further comprises nucleic acid for a nucleic acid amplification, the temperature variation area has a temperature necessary at least for a nucleic-acid amplification, and the nucleic acid amplification is conducted by moving the droplet to a spot in the temperature variation area controlled to keep at least one temperature necessary for the nucleic-acid amplification, wherein the nucleic acid is attached to surfaces of the magnetic body particles by contacting the magnetic body particles with the hydrophilic surfaces with a sample including the nucleic acid inside the droplet having nucleic-acid extraction liquid for extracting the nucleic acid from the sample, wherein a droplet inclusion medium is filled in the reaction receptacle or contacted with the surface of the reaction substrate in such a way as to be layered, said droplet inclusion medium is insoluble in the aqueous solution forming the droplet, the droplet is confined in the droplet inclusion medium, and the droplet contacts a surface of a wall inside the reaction receptacle or the reaction substrate and is displaced due to the fluctuations of the magnetic field, and wherein the droplet inclusion medium has a melting point lower than a temperature for conducting the chemical reaction, the droplet inclusion medium is in a solid state and fixes the droplet before conducting the chemical reaction, and the droplet inclusion medium is in a liquid state and enabling the droplet to move when the chemical reaction is conducted.