Patent ID: 8128803

Claim:
A method for electrochemical detection of an analyte, comprising: exposing the analyte to an enzyme attached to a substrate so that a hydrolytic cleavage process occurs between the enzyme and the substrate after which the enzyme is bound directly or indirectly to the analyte or displaced by the analyte, the enzyme having a post-cleavage concentration representing a concentration of the analyte; converting the substrate and forming a reporter molecule during the hydrolytic cleavage process, the reporter molecule being capable of reverse electrochemical conversion under conditions in which the substrate is stable; polarizing an electrode of a sensor during a measurement phase so that the reporter molecule is oxidized or reduced respectively to an oxidized form or a reduced form of the reporter molecule, the electrode being oppositely polarized during a relaxation phase so that the oxidized form or the reduced form of the reporter molecule is respectively reduced or oxidized at the electrode; and recording a current flowing to or from the electrode during the measurement phase to measure a concentration of the reporter molecule formed during the hydrolytic cleavage process.