Patent ID: 7092825

Claim:
A method of protein engineering including the steps of:— (i) creating a computer database which includes a plurality of entries, each said entry corresponding to a description of a location and orientation in 3D space of side chains of amino acid residues of a framework protein which comprises 70 amino acids or less and has 1–11 disulfide bonds, wherein the location and orientation of each side chain is represented by a C α –C β vector; (ii) creating a query corresponding to a description of a location and orientation in 3D space of each side chain of two or more amino acid residues of a sample protein which are required for a function of said sample protein, wherein the location and orientation of each side chain is represented by a C α –C β vector; (iii) searching said database with said query to thereby identify one or more hits wherein at least one of said hits corresponds to a respective said framework protein which has 3D structural similarity to said sample protein; (iv) identifying the amino acid residue(s) of the framework protein hit identified at step (iii) that structurally correspond(s) to each of the two or more amino acid residues of said sample protein used to create the query at step (ii); (v) determining which amino acid residue(s) identified at step (iv) is/are to be substituted by another amino acid to impart a particular property, characteristic or function to the framework protein identified as a hit; and (vi) modifying said framework protein which corresponds to a hit, by substituting at least one amino acid residue thereof determined at step (v) with another amino acid to thereby create an engineered framework protein having the particular property, characteristic or function.