Patent ID: 8642322

Claim:
A method of measuring an amount of an analyte in a sample, the method comprising: displacing the sample from a sample inlet to a first conduit comprising a sensor; contacting the sample with the sensor; contacting an enzyme-labeled antibody with the sensor, wherein the enzyme-labeled antibody is configured to bind the analyte in the sample; displacing the sample from the sensor into a waste region; providing a solution in a second conduit, wherein the solution comprises a substrate for the enzyme-labeled antibody; displacing the solution from the second conduit into the first conduit, wherein the displacing the solution comprises forming at least one air segment in the solution; contacting the solution and the at least one air segment with the sensor to remove unbound analyte and labeled antibody from a region of the sensor; and detecting a product of a reaction between the enzyme-labeled antibody and the substrate using the sensor.