Patent ID: 8592158

Claim:
A method of determining the length of a multinucleotide repeat region in a target nucleic acid, comprising: amplifying a target nucleic acid to produce amplified target nucleic acids; labeling the amplified target nucleic acids with a first label, the first label independent of the number of multinucleotide repeats; binding the amplified target nucleic acids to a first capture probe specific for the amplified target nucleic acids, the first capture probe attached to a first encoded particle characterized by a first code; amplifying the target nucleic acid to produce multinucleotide repeat region nucleic acids; labeling the multinucleotide repeat region nucleic acids with a second label, the second label proportional to the number of multinucleotide repeats; binding the multinucleotide repeat region nucleic acids to a second capture probe specific for the multinucleotide repeat region nucleic acids, the second capture probe attached to a second encoded particle characterized by a second code, wherein the first and second codes are distinguishable; detecting the first code and the first label associated with the first capture probe to produce a first signal; detecting the second code and the second label associated with the second capture probe to produce a second signal; and determining a ratio of the first signal and the second signal, wherein the ratio is indicative of the length of the multinucleotide repeat in the target nucleic acid.