Patent ID: 8871515

Claim:
A method for propagating a fully-deleted adenoviral-based gene transfer vector comprising: (a) providing an Adenovirus packaging cell line; (b) transfecting, into the cell line, a fully-deleted Adenoviral vector construct; and (c) transfecting, into the cell line, a replication defective circular packaging construct having a subset of Adenoviral late genes including L1, L2, L3, L4, L5, E2A, and E4, while being absent of at least one inverted terminal repeat and a packaging signal, wherein the fully-deleted Adenoviral vector construct and the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted adenoviral-based gene transfer vector independent of helper Adenovirus, wherein the encapsidated fully-deleted adenoviral-based gene transfer vector includes both adenoviral inverted terminal repeats, the packaging signal, and at least one DNA insert which comprises a gene sequence encoding a protein of interest, and wherein the encapsidated fully-deleted adenoviral-based gene transfer vector is absent of adenoviral structural genes.