Patent ID: 8337683

Claim:
A microfluidic sensor complex structure, comprising: a lower plate, on which a reference electrode, a working electrode and an electrode connection are formed; wherein the lower plate is further provided with one or more verifying electrodes configured to minimize the deviation of detected signals, wherein the verifying electrodes are comprised of a first verifying electrode, for measuring a background signal, and a second verifying electrode for detecting a saturation signal of the saturated or partially saturated enzyme conjugate, a middle plate, overlaid on the lower plate, comprising therein: a sample inlet channel; a microfluidic channel passage, which extends from the sample inlet channel and serves as a guide along which a sample flows over the entire middle plate, and which is divided at a position near the sample inlet channel into two branches, on which an enzyme conjugate reservoir and a substrate reservoir are positioned, respectively, said two branches being confluent before a detection channel, at which the reference electrode and the working electrode are exposed; a mixing channel, positioned before a position of confluence on the microfluidic channel passage extending through the substrate reservoir, comprising an air discharge channel such that a sample flowing through the substrate reservoir reaches the detection channel later than does a sample flowing through the enzyme conjugate reservoir; an absorbing channel in which the sample fluid flowing out of the detection channel is absorbed; an air inlet channel provided at an end of the absorbing channel; and an upper plate, overlaid on the middle plate so as to induce a capillary phenomenon on the microfluidic channel passage formed on in the middle plate.