Patent ID: 7374939

Claim:
A method of site specific insertion or deletion in Zymomonas comprising: (a) providing a Zymornonas DNA fragment; (b) interrupting a sequence in the Zymomonas DNA fragment to provide an interrupted sequence formed of a first component part and a second component part of the Zymomonas DNA fragment; (c) recombining the interrupted sequence with a desired sequence to form a recombinant gene fragment wherein the first component part and the second component part of the Zyrnornonas DNA fragment respectively flank the desired sequence to facilitate site specific insertion or deletion, the first component part and the second component part targeting a by-product of a Zymomonas metabolic pathway; and (d) transforming the Zymomonas to induce site specific insertion or deletion by homologous recombination with the interrupted sequence to provide a recombinant Zymomonas , wherein said recombinant gene fragment provides for elimination of a by-product of a metabolic pathway in the recombinant Zymomon as; and further comprising (e) ligating the interrupted sequence with a plasmid vector; wherein the step (d) of the transforming the Z. mobilis organism occurs through homologous recombination with the interrupted sequence of the plasmid vector; wherein the plasmid is selected from the group consisting of pZB 101, pZB 102, and pZB 121.