Patent ID: 7312033

Claim:
A kit comprising a detection assay configured for detecting the ΔF508 CFTR allele, wherein said detection assay is able to discriminate between ΔI507 and said ΔF508 CFTR allele, wherein said detection assay comprises first and second oligonucleotides configured to form an invasive cleavage structure in combination with a target sequence comprising said ΔF508 CFTR allele, wherein said first oligonucleotide comprises a 5′ portion and a 3′ portion, wherein said 3′ portion is configured to hybridize to said target sequence, wherein said 5′ portion is configured to not hybridize to said target sequence, and wherein said second oligonucleotide comprises a 5′ portion and a 3′ portion, wherein said 5′ portion is configured to hybridize to said target sequence, and wherein said 3′ portion is configured to not hybridize to said target sequence, and wherein said 3′ portion of said first oligonucleotide consists of: i) the following sequence: 3′-ACCGTGGTAATTTCTTTTATAATAAC-5′, and ii) a 3′ blocking group; and wherein the 5′ terminal base of said sequence is attached to said 5′ portion of said first oligoniucleotide.