Patent ID: 8575434

Claim:
A DNA construct comprising: a first, second, third and fourth expression cassette, wherein said first expression cassette in operable linkage comprises: (a) a maize ubiquitin promoter; (b) a 5′ untranslated exon of a maize ubiquitin gene; (c) a maize ubiquitin first intron; (d) a Cry1F encoding DNA molecule; and (e) a poly(A) addition signal from ORF 25 terminator; said second expression cassette in operable linkage comprises: (1) a maize ubiquitin promoter; (2) a 5′ untranslated exon of a maize ubiquitin gene; (3) a maize ubiquitin first intron; (4) a Cry34Ab1 encoding DNA molecule; and (5) a PinII transcriptional terminator; said third expression cassette in operable linkage comprises; (i) a wheat peroxidase promoter; (ii) a Cry35Ab1 encoding DNA molecule; and (iii) a PinII transcriptional terminator; and said fourth expression cassette in operable linkage comprises; (a) a CaMV 35S promoter; (b) a pat encoding DNA molecule; and (c) a 3′ transcriptional terminator from CaMV 35S; wherein the four cassettes are flanked by SEQ ID NO: 27 at the 5′ end and SEQ ID NO: 28 at the 3′ end.