Patent ID: 7057090

Claim:
A method of producing a transgenic turfgrass plant, comprising the steps of: (a) culturing embryogenic tissue from seeds of a turfgrass plant on a medium that promotes de-differentiation of the tissue, to produce regenerable callus tissue; (b) inoculating the callus tissue with Agrobacterium carrying at least one vector for transformation, the vector comprising virB, virG and virG virulence genes from plasmid pSB1 or pSB4, in which vector is inserted a heterologous DNA construct and a selectable marker conferring antibiotic resistance to transformed cells, wherein the DNA construct and selectable marker are operably linked to a promoter from a monocotyledonous species, wherein the inoculating comprises mixing the callus tissue with the Agrobacterium pre-incubated with acetosyringone, under conditions permitting the Agrobacterium to infiltrate the callus tissue, thereby forming Agrobacterium -infiltrated callus tissue; (c) co-culturing the Agrobacterium -infiltrated callus tissue under conditions that enable the Agrobacterium vector to transform cells of the Agrobacterium -infiltrated callus tissue; (d) selecting transformed cells by culturing the Agrobacterium -infiltrated callus tissue on a selection medium comprising an antibiotic, wherein the transformed cells are resistant to the antibiotic and are selected by their growth in the presence of the antibiotic; and (e) regenerating a transformed turfgrass plant from the transformed cells.