Patent ID: 8658359

Claim:
A method of disrupting cells and purifying nucleic acids using a single chip, the method comprising: introducing a cell-containing sample solution to a solid support, wherein the solid support comprises a cell lysis enhancing metal oxide layer deposited on a surface of the solid support and a transparent cell binding metal oxide layer deposited on the cell lysis enhancing metal oxide layer, wherein the cells bind with the cell binding metal oxide; irradiating the solid support with a laser to disrupt the cells bound to the cell binding metal oxide to release a nucleic acid that binds with the cell binding metal oxide, wherein the cell lysis enhancing metal oxide layer enhances absorption of light irradiated by the laser; binding the released nucleic acid to the cell binding metal oxide layer; introducing a washing buffer to the solid support to remove the cell-containing sample solution that is not bound to the cell binding metal oxide; and introducing a nucleic acid eluting buffer to the solid support to elute the nucleic acid bound to the cell binding metal oxide, wherein the cell lysis enhancing metal oxide is Fe 2 O 3 , and the cell binding metal oxide is selected from the group consisting of Al 2 O 3 , TiO 2 , Ta 2 O 3 , Fe 3 O 4 and HfO 2 .