Patent ID: 7872104

Claim:
An isolated monoclonal antibody, wherein said monoclonal antibody binds with high affinity to both mature and zymogen forms of native human single-chain intact prostate specific antigen (SCINT PSA) and said monoclonal antibody does not bind to a native nicked PSA (PSA-N), characterized in that said monoclonal antibody is obtained through immunization with an uncleaved form of prostate specific antigen (SCINT PSA), is selected by its differential binding to the intact and internally cleaved forms, wherein said SCINT PSA is prostate specific antigen (PSA) not internally cleaved between amino acids lysine 145 and lysine 146, wherein said PSA-N has been formed by internal peptide bond cleavage(s) of SCINT PSA resulting in two-chain or multi-chain PSA and wherein the cleavage of PSA-N has occurred between amino acids lysine 145 and lysine 146 of the PSA.