Patent ID: 8614061

Claim:
A method for the simultaneous detection of at least four nucleic acids in a sample, each nucleic acid labelled with a covalently attached fluorescent dye, the method comprising detecting the fluorescence emission of each of the fluorescent dyes upon excitation, wherein (i) a first fluorescent dye selected from the group consisting of 5-FAM and 6-FAM or a blend thereof is covalently attached to a first nucleic acid, (ii) a second fluorescent dye selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532 is covalently attached to a second nucleic acid, (iii) a third fluorescent dye selected from the group consisting of ATTO 550, DY-555 and DY-556 covalently attached to a third nucleic acid, and (iv) a fourth fluorescent dye selected from the group consisting of ROX, DY-510 XL, and ATTO 565 is covalently attached to a fourth nucleic acid, and (v) optionally a fifth fluorescent dye selected from the group consisting of DY-632 and DY-520 XL is covalently attached to a fifth nucleic acid.