Patent ID: 8592348

Claim:
A method for producing a chip on which biomolecules are immobilized in an aligned state, which comprises the following steps (a) to (e): (a) producing a substrate 1 on which a plurality of biomolecules 1 of a single type are immobilized in an aligned state; wherein said biomolecules 1 are cDNA; (b) filling microreactors on a microreactor chip comprising the microreactors at positions overlapping with the sequence positions of the cDNA biomolecules 1 immobilized on the substrate 1 produced in the step (a), with reaction reagents for synthesizing biomolecules 2 ; wherein said biomolecules 2 are mRNA; (c) closely attaching the microreactor chip to the substrate 1 so that the reaction reagents for synthesizing the mRNA biomolecules 2 are allowed to come into contact with the cDNA biomolecules 1 , and then carrying out a reaction of synthesizing the mRNA biomolecules 2 in the microreactors so as to synthesize the mRNA biomolecules 2 in reaction solutions contained in the microreactors; (d) superposing the microreactor chip on a substrate 2 so that the reaction solutions contained in the microreactors on the microreactor chip are allowed to come into contact with the substrate 2 after completion of the step (c), so as to immobilize the mRNA biomolecules 2 on the substrate 2 ; wherein linker DNA is bound to said mRNA biomolecules 2 and a compound that specifically binds to a protein is bound to said linker DNA; and (e) immersing the substrate 2 in reaction reagents for synthesizing biomolecules 3 from the mRNA biomolecules 2 immobilized on the substrate 2 ; wherein said biomolecules 3 are protein-nucleic acid complexes in which said protein is bound to said compound.