Patent ID: 8058033

Claim:
A glucoamylase variant comprising amino acid substitutions corresponding to positions: 417, 430, 511, 539, and 563 of SEQ ID NO: 2, or equivalent positions in a parent glucoamylase, wherein the glucoamylase variant has at least 90% sequence identity with a glucoamylase selected from the group consisting of the Trichoderma reesei glucoamylase comprising SEQ ID NO: 1, 2, or 3, the Aspergillus awamori glucoamylase comprising SEQ ID NO: 5 and SEQ ID NO: 168, the Aspergillus niger glucoamylase comprising SEQ ID NO: 6 and SEQ ID NO: 167, the Humicola grisea glucoamylase comprising SEQ ID NO: 8 and SEQ ID NO: 163, and the Hypocrea vinosa glucoamylase comprising SEQ ID NO: 9.