Patent ID: 7771929

Claim:
A method of performing a multiplexed assay for the determination of a plurality of target proteins in a sample, the method comprising the steps of: providing a binding compound consisting of an antibody or antibody fragment thereof for each of the plurality of target proteins, each binding compound having one or more eTag reporters attached thereto by a cleavable linkage, the one or more eTag reporters of each different binding compound having a different electrophoretic mobility after cleavage so that eTag reporters of each different binding compound form distinct peaks after electrophoretic separation; combining with the sample the binding compound and a second binding compound consisting of an antibody or antibody fragment thereof for each of the plurality of target proteins, wherein the second binding compound is conjugated with an active species producing moiety for generating an active species for oxidizing the cleavable linkage, such that in the presence of a target protein a complex is formed between each target protein and the binding compound(s) specific therefor; cleaving each cleavable linkage by oxidation where said step includes providing the active species consisting of singlet oxygen for oxidizing the cleavable linkage thereby so that eTag reporters are released; and electrophoretically separating and identifying the released eTag reporters to determine the plurality of target proteins in the sample.