Patent ID: 7811571

Claim:
A polyallylamine conjugate, consisting of polyallylamine, a signal molecule, and a capture agent, wherein said signal molecule is a fluorescent molecule which is cy5; said capture agent is a modified antibody linked to a cross-linking agent, wherein the antibody is modified by a compound having functional groups selected from the group consisting of —SH, —CO, —NH 2 and —N═C═S, and a cross-linking agent selected from the group consisting of N-succinimidyl 3-(2-pyridyldithio) propionate, glutaraldehyde, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), maleimide, iodoacetamide, protected thiol and sulfosuccinimidyl-6-(3′-[2-pyridyldithio] propionamido) hexanoate (sulfo-LC-SPDP), and wherein said cross-linking agent assists the binding of said capture agent with the —NH 2 functional groups of polyallylamine; and the signal molecule and capture agent is covalently bound to the polyallylamine via —NH 2 side chain of the polyallylamine.