Patent ID: 8455202

Claim:
A multi-dimensional method for simultaneously analyzing multiple protein analytes within a sample solution, the method comprising: adding affinity selectors to a sample solution containing protein analytes to be measured, the affinity selectors having an affinity for one or more of the analytes within the sample solution, wherein the affinity selectors include at least one of an antibody and an antibody fragment; allowing immune complexes to form between the affinity selectors and the protein analytes; partially or totally resolving the formed immune complexes from non-analyte substances initially within the sample solution; dissociating the resolved immune complexes to form polypeptide analytes from proteolysis of the protein analytes; separating the polypeptide analytes derived from the immune complexes from one another by capturing the polypeptide analytes through a surface adsorption process; transferring the captured polypeptide analytes to a detection means; and resolving the polypeptide analytes with the detection means according to their mass-to-charge ratios.