Patent ID: 8513394

Claim:
A fluorescent enzymatic substrate comprising: a backbone S of saccharide nature comprising at least one saccharide unit selected from the group consisting of monosaccharides, oligosaccharides having from 2 to 9 saccharide units, and polysaccharides having at least 10 saccharide units; B 1 and B 2 connected to the backbone S, B 1 and B 2 being identical or different, each B 1 and B 2 representing a spacer arm comprising a linear or branched, saturated or unsaturated hydrocarbon-based chain optionally substituted, interrupted and/or ending with one or more heteroatoms selected from the group consisting of N, O and S, and/or with one or more groups selected from the group consisting of C 1 -C 4 alkyl, C 1 -C 4 alkoxy and aryl radicals or with one or more functions selected from the group consisting of ether, ester, amide, carbonyl, carbamate, urea, thiourea and disulfide functions, wherein B 1 and B 2 are present or absent, independently of one another; a fluorophore group F 1 ; and an inhibitor I 1 of the fluorescence of F 1 ; wherein the fluorophore group F 1 and the inhibitor I 1 do not comprise a saccharide structure; the backbone S comprises at least one monosaccharide comprising F 1 and I 1 grafted thereto, either directly or by the spacer arms B 1 and B 2 , respectively, when at least one B 1 and B 2 is present; one of the groups F 1 and I 1 is grafted in the anomeric position 1 of said monosaccharide, the anomeric bond being without distinction in the α- or β-position, the other group F 1 or I 1 occupying any free position of the same saccharide unit; and no phosphate chain, saccharide unit or nitrogenous base intercalates between the saccharide unit of the backbone S bearing the groups F 1 and I 1 , and said groups F 1 and I 1 .