Patent ID: 7030292

Claim:
A method for producing a mammalian homozygous embryonic stem (HES) cell depository, wherein each HES cell produces a cell line, which has alleles that are homozygous for a Major Histocompatibility Complex (MHC) haplotype, wherein the method comprises: (a) selecting donors; (b) determining the MHC haplotype of each donor; (c) mitotically activating non-fertilized post-meiosis I diploid oocytes obtained from each donor to develop multiple blastocyst-like masses, each of which contains an inner cell mass (ICM) that is homozygous for a particular MHC haplotype, wherein the non-fertilized post-meiosis I diploid oocytes are subject to sham intracytoplasmic sperm injection (ICSI) followed by incubation with calcium ionophore; (d) isolating HES cells from the ICM obtained from each donor; (e) culturing the isolated HES cells to obtain HES cell lines; (f) determining the MHC haplotype of each HES cell line; and (g) cataloging the MHC haplotype of each HES cell line obtained in (e).