Patent ID: 6951753

Claim:
A helper-free recombinant adeno-associated virus stock, said recombinant adeno-associated virus stock comprising a packaged recombinant adeno-associated virus vector containing a foreign DNA sequence, but lacking adeno-associated virus genes required for replication or packaging of said adeno-associated virus vector, wherein said adeno-associated virus is produced by cells permissive for adeno-associated virus replication, said cells cotransfected with (i) a recombinant adeno-associated virus vector comprising a foreign DNA sequence but lacking adeno-associated virus genes required for replication or packaging of said adeno-associated virus vector, and (ii) a recombinant helper adeno-associated virus DNA comprising adeno-associated virus genes required for replication or packaging, non-adeno-associated virus cis acting elements and lacking adeno-associated virus inverted terminal repeat sequences, wherein said cotransfected cells are infected with a helper virus. wherein said recombinant adeno-associated virus vector comprises up to 195 base pairs of the adeno-associated virus terminal repeated sequence, and shares no adeno-associated virus sequence with the helper adeno-associated virus DNA.