Patent ID: 7306906

Claim:
A method of amplifying nucleic acid comprising: a) annealing a primer to a template, the primer having a first portion which anneals to the template and a second portion of predetermined sequence which does not anneal to the template and is not native to the template; b) synthesizing a polynucleotide that is complementary to the portion of the template between the location at which the first portion of the primer anneals to the template and the end of the template, the polynucleotide having the primer at a first end thereof and a second end; c) separating the polynucleotide synthesized in step (b) from the template; d) annealing a template oligonucleotide to the second end of the polynucleotide synthesized in step (b), the template oligonucleotide having a first portion that anneals to the second end of the polynucleotide and a second portion having the same predetermined sequence as the second portion of the primer; e) extending the polynucleotide synthesized in step (b) to provide a terminal portion thereof that is complementary to the predetermined sequence; and f) amplifying the extended polynucleotide using a single primer having the predetermined sequence.