Patent ID: 8470595

Claim:
A method for producing a myoblast by culturing mouse-derived pluripotent stem cells, the method comprising: i) preparing the mouse-derived pluripotent stem cells that have been cryopreserved; ii) sub-culturing the prepared mouse-derived pluripotent stem cells 8 to 12 times in an undifferentiated state, wherein the mouse-derived pluripotent stem cells are sub-cultured in a medium containing leukemia inhibitory factor (LIF); iii) culturing the subcultured mouse-derived pluripotent stem cell under conditions that enable induction of differentiation into an adipocyte in vitro, wherein the conditions include suspension-culturing in a medium containing retinoic acid for 2 to 3 days, followed by culturing in a medium containing insulin and triiodothyronine (T3), and wherein the time period of the iii) culturing is 7 to 21 days from the start of the culturing following the ii) sub-culturing; iv) separating and collecting a CD105-positive mesenchymal stem cell after the culturing in the medium containing insulin and T3; and v) further culturing the separated and collected CD105-positive cell under conditions that enable induction of differentiation into a myoblast in vitro, wherein the collected CD105-positive cells are cultured in ES cell culture medium containing serum or serum replacement.