Patent ID: 7892752

Claim:
A method of identifying and/or quantifying one or more glycosylation markers of a cancer, comprising (A) obtaining a glycoprotein-containing sample from a subject diagnosed with the cancer, wherein the glycoprotein-containing sample contains plural types of glycoproteins; (B) immobilizing total glycoproteins of the sample from the subject on a protein binding membrane or in a gel without having isolated or purified specific glycoproteins of the sample; (C) releasing glycans of the total immobilized glycoproteins on the protein binding membrane or in the gel without the gel having been separated into a plurality of bands prior to said releasing; (D) washing the protein binding membrane or the gel to separate the released glycans from parent proteins immobilized on the protein binding membrane or in the gel; (E) measuring a glycosylation profile of the released glycans; and (F) comparing the glycosylation profile with a control profile to determine the one or more glycosylation markers of the cancer.