Patent ID: 7709253

Claim:
A ligand-regulable transactivation system comprising: a reporter polynucleotide that comprises a GAL4 binding sequence of SEQ. ID NO: 17, a promoter sequence, and a reporter sequence encoding a detectable bioluminescent protein, wherein the binding sequence is connected with the promoter sequence and the promoter sequence is connected with the reporter sequence; and an activator fusion protein that comprises a DNA binding domain capable of interacting with the GAL4 binding sequence of the reporter polynucleotide, an estrogen receptor folding domain (ER folding domain) capable of binding a compound, and a transactivation domain capable of interacting with the promoter sequence of the reporter polynucleotide, wherein the DNA binding domain is connected to the estrogen receptor folding domain, and the estrogen receptor folding domain is connected with the transactivation domain, and wherein interaction of the DNA binding domain and the transactivation domain of the activator fusion protein with the binding sequence and promoter sequence of the reporter polynucleotide regulates expression of the detectable bioluminescent protein.