Patent ID: 7833185

Claim:
An apparatus for separating a volume of whole blood contained in a separation bag into at least a plasma component, a mononuclear cell component and a red blood cell component, the separation bag being fluidly connected to at least a plasma component bag and a mononuclear cell component bag, the apparatus comprising: a rotor for spinning the separation container around a rotation axis of the rotor; a container on the rotor for holding at least the plasma component bag and the mononuclear cell bag; a first valve member mounted on the rotor for interacting with a first tube connecting the separation bag to the plasma component bag and selectively allowing or blocking a fluid flow therethrough; a second valve member mounted on the rotor for interacting with a second tube connecting the separation bag to the mononuclear cell component bag and selectively allowing or blocking a fluid flow component therethrough; at least one sensor for detecting red blood cells in the separation bag at a distance from the rotation axis of the rotor; a fluid transfer system for causing the transfer of the plasma component from the separation bag into the plasma component bag and the transfer of the mononuclear cell component from the separation bag into the mononuclear cell component bag; a controller with a memory configured for storing at least one centrifugation speed allowing for the sedimentation of a volume of whole blood into a first inner layer comprising plasma, a second intermediate layer comprising platelets, a third intermediate layer comprising lymphocytes, monocytes and granulocytes and a fourth outer layer comprising red blood cells, wherein at least the third and fourth layers partially overlap and the granulocytes are in part embedded in the fourth layer; and a control unit programmed: for receiving an information from the at least one sensor, and the at least one centrifugation speed from the memory; for causing the rotor to rotate at the at least one centrifugation speed; for causing the first valve member to open the first tube connected to the plasma component bag and the second valve member to close the second tube connected to the mononuclear cell component bag, when the volume of whole blood has sedimented in the separation bag; for causing the fluid transfer system to transfer into the plasma component bag a plasma component comprising a first fraction of the first layer; for causing, when red blood cells are detected by the at least one sensor, the fluid transfer system to stop transferring a content of the separation bag, the first valve member to close the first tube connected to the plasma component bag and the second valve member to open the second tube connected to the mononuclear cell component bag; and for causing the fluid transfer system to transfer, into the mononuclear cell component bag, a mononuclear cell component having a predetermined volume and comprising at least a fraction of the third layer comprising lymphocytes and monocytes.