Patent ID: 8007789

Claim:
Isolated antibiotic GE 81112 Factor B having the following characteristics: A) Mass spectrum recorded from a 0.01 mg/ml solution in acetonitrile:water 50:50 giving a monoprotonated ion at 659 m/z on a Thermofinnigan LCQ deca instrument under the following electrospray conditions: Spray Voltage: 4.7 kv; Capillary temperature: 250° C.; Capillary Voltage: 9V; Infusion mode 5 μl/min. B) Infrared spectrum ( FIG. 6 ) recorded in KBr with an IFS-48 Fourier Transform spectrophotometer showing the following main bands (cm −1 ): 3359; 2958; 2852; 2258; 2129; 1681; 1591; 1450; 1385; 1347; 1122; 1072; 1025; 998; 765 C) 1 H-NMR spectrum ( FIG. 7 ) recorded at 600 MHz at 25° C. in DMSO-d 6 and trifluoroacetic acid (drops) showing the following 1 H signals: Chemical shift (ppm) Multiplicity 1.52 br d 1.57-1.72 m 1.83 br d 1.90 m 2.63 dd 2.88 dd 2.92 m 3.13 br d 3.64 m 3.83 m 3.95 br d 4.35 br s 4.46 m 4.50 dd 4.60 m 5.05 d 6.46 br 6.48 s 6.93 s 7.40 br s 7.84 d 8.05 d 8.33 m 8.54 d 9.00 br d 11.72 br s 11.75 br s — — D) 13 C-NMR spectrum ( FIG. 8 ) recorded at 150 MHz, at 25° C. in DMSO-d 6 and trifluoroacetic acid (drops) showing the following 13 C signals 13 C Chemical shift (ppm) Multiplicity 15.9 t 27.36 t 28.40 t 35.47 t 42.87 t 50.1 d 51.20 d 56.78 d 59.73 d 64.09 d 65.14 d 66.90 d 67.77 t 110.3 d 117.85 d 122.5 s 128.4 s 139.66 s 146.7 s 156.7 s 167.4 s 170.5 s 171.3 s 171.38 s E) Retention time 21.6 min, determined by using an analytical HPLC Shimadzu LC10-AS, equipped with an “Altima” RP18, 5 μm, 250×4.6 mm i.d column; isocratic elution performed at 1 ml/min. flow rate, with 40 mM ammonium formate buffer, brought to pH 4.5 by addition of formic acid; UV detection at 230 nm.