Patent ID: 6936687

Claim:
A fluorogenic composition for the detection of the activity of a protease, said composition having the formula: wherein, P has the sequence Leu-Glu-His-Asp-Gly-Ile-Asn (SEQ ID NO:212); F 1 and F 2 are fluorophores and F 1 is attached to the amino terminal amino acid and F 2 is attached to the carboxyl terminal amino acid; S 1 and S 2 , when present, are peptide spacers ranging in length from 1 to about 50 amino acids and S 1 , when present, is attached to the amino terminal amino acid and S 2 , when present, is attached to the carboxyl terminal amino acid; i, j, k, l, m, n, o, p, q, and r are independently 0 or 1; aa 1 and aa 10 are independently selected from the group consisting of lysine, ornithine and cysteine; aa 2 and aa 3 are independently selected from the group consisting of Asp, Glu, Lys, Ornithine, Arg, Citulline, homocitrulline, Ser, Pro, homoserine, Aib, Thr, and Tyr; aa 8 and aa 9 are independently selected from the group consisting of Asp, Glu, Lys, Ornithine, Arg, Citulline, homocitrulline, Ser, homoserine, Thr, and Tyr; aa 5 , aa 4 , aa 6 , and aa 7 are independently selected from the group consisting of proline, 3,4-dehydroproline, hydroxyproline, alpha aminoisobutyric acid and N-methyl alanine; X is selected from the group consisting of Gly, βAla, γAbu, Gly-Gly, Ahx, 8-aminocaprylic acid, βAla-Gly, βAla-βAla, γAbu-Gly, βAla-γAbu, Gly-Gly-Gly, γAbu-γAbu, Ahx-Gly, βAla-Gly-Gly, Ahx-βAla, βAla-βAla-Gly, Gly-Gly-Gly-Gly (SEQ ID NO:223), Ahx-γAbu, βAla-βAla-βAla, γAbu-βAla-Gly, γAbu-γAbu-Gly, Ahx-Ahx, γAbu-γAbu-βAla, and Ahx-Ahx-Gly, Y is selected from the group consisting of Gly, βAla, γAbu, Gly-Gly, Ahx, 8-aminocaprylic acid, Gly-βAla, βAla-βAla, Gly-γAbu, γAbu-βAla, Gly-Gly-Gly, γAbu-γAbu, Gly-Ahx, Gly-Gly-βAla, βAla-Ahx, Gly-βAla-βAla, Gly-Gly-Gly-Gly (SEQ ID NO:223), γAbu-Ahx, βAla-βAla-βAla, Gly-βAla-γAbu, Gly-γAbu-γAbu, Ahx-Ahx, βAla-γAbu-γAbu, and Gly-Ahx-Ahx; when i is 1, S 1 is joined to aa 1 by a peptide bond through a terminal alpha amino group of aa 1 ; and when r is 1, S 2 is joined to aa 10 by a peptide bond through a terminal alpha carboxyl group.