Patent ID: 8309329

Claim:
A method for producing 5′-guanylic acid comprising: A) reacting inosinic acid with a microorganism which is able to convert inosinic acid into 5′-guanylic acid in a reaction solution comprising an organic solvent, wherein permeability of said microorganism's cells to nucleotide is activated by the presence of an organic solvent; and B) collecting 5′-guanylic acid; wherein said microorganism has been modified so that inosinic acid dehydrogenase activity and 5′-guanylic acid synthetase activity are enhanced by increasing expression of a guaB gene and guaA gene, wherein said guaA gene encodes a protein selected from the group consisting of: (A) a protein comprising the amino acid sequence of SEQ ID NO: 2, and (B) a protein comprising an amino acid sequence which has a sequence identity of not less than 95% to that of SEQ ID NO: 2, and has 5′ guanylic acid synthetase activity, wherein said guaB gene encodes a protein selected from the group consisting of: (C) a protein comprising the amino acid sequence of SEQ ID NO: 10, and (D) a protein comprising an amino acid sequence which has a sequence identity of not less than 95% to that of SEQ ID NO: 10, and has inosinic acid dehydrogenase activity, and wherein said microorganism is selected from the group consisting of Escherichia bacteria, Bacillus bacteria and coryneform bacteria.