Patent ID: 8217187

Claim:
A method of processing quassinoid(s) from plant material selected from the group consisting of Picrasma excelsa, Quassia amara, Picrasma quassioides, Quassia africana and mixtures thereof comprising: adding a first solvent to said plant material; mixing said first solvent and said plant material; evaporating said first solvent to yield a residue; adding water to said residue and washing with a second solvent, to yield an aqueous fraction; saturating said aqueous fraction with a polarity increasing agent and extracting with ethyl acetate to yield a second residue; subjecting said second residue to crystallization to yield crystalline quassinoid(s) and mother liquor; separating said mother liquor with column chromatography; and subjecting said separated mother liquor to crystallization to yield additional crystalline quassinoid(s).