Patent ID: 7416875

Claim:
An engineered bovine pancreatic ribonuclease A (RNase A) wherein said engineered bovine ribonuclease A is selected from the group consisting of: (i) an RNase A variant different from the native bovine pancreatic ribonuclease A of SEQ ID NO: 1 at amino acid residue positions 39 and 88, wherein the difference consists of an arginine at position 39 being changed to an aspartic acid, and a glycine at position 88 being changed to an arginine, (ii) an RNase A variant different from the native bovine pancreatic ribonuclease A of SEQ ID NO: 1 at amino acid residue positions 67 and 88, wherein the difference consists of an asparagine at position 67 being changed to an arginine, and a glycine at position 88 being changed to an arginine, (iii) an RNase A variant different from the native bovine pancreatic ribonuclease A of SEQ ID NO: 1 at amino acid residue positions 38, 39 and 88, wherein the difference consists of an aspartic acid at position 38 being changed to an arginine, an arginine at position 39 being changed to an aspartic acid, and a glycine at position 88 being changed to an arginine, and (iv) an RNase A variant different from the native bovine pancreatic ribonuclease A of SEQ ID NO: 1 at amino acid residue positions 38, 39, 67 and 88, wherein the difference consists of an aspartic acid at position 38 being changed to an arginine, an arginine at position 39 being changed to an aspartic acid, an asparagine at position 67 being changed to an arginine, and a glycine at position 88 being changed to an arginine.