Patent ID: 8048632

Claim:
A method of detecting the presence of DNA corresponding to the genomic/transgene DNA of canola PV-BNGT04(RT73) and progeny thereof in a sample, the method comprising: (a) contacting the sample comprising canola DNA with a primer pair, that when used in a nucleic acid amplification reaction with DNA from canola PV-BNGT04(RT73) and progeny thereof produces an amplicon that is diagnostic for canola PV-BNGT04(RT73) and progeny thereof, wherein said primer pair comprises a first primer comprising at least 11 contiguous nucleotides from nucleotide 1-236 of the transgene region of SEQ ID NO:8 or full complements thereof, and a second primer comprising at least 11 contiguous nucleotides from nucleotide 237-474 of the 3′ flanking genomic region of SEQ ID NO:8 or full complements thereof; and (b) performing the nucleic acid amplification reaction, thereby producing the amplicon; comprising SEQ ID NO:6 and (c) detecting the amplicon.