Patent ID: 8563328

Claim:
A biosensing method for simultaneously detecting and quantifying two or more target biomarkers, said method comprising: providing at least two optical waveguides, each of the at least two optical waveguides being disposed at least partially in a respective microfluidic channel of a serpentine shaped chamber, the at least two optical waveguides being fluidly coupled to one another in series within the serpentine shaped chamber; selecting 1° Mabs corresponding to each of the two or more target biomarkers; immobilizing the 1° Mabs on the optical waveguides; exposing to the 1° Mabs a sample believed to contain the target biomarkers using convective flow within the serpentine shaped chamber, wherein the sample flows through the serpentine shaped chamber and flows over each of the at least two optical waveguides in series; providing fluorophore-tagged 2° Mabs corresponding to the target biomarkers; exciting the at least two optical waveguides; and observing a fluorescence emission to determine the presence and quantity of the target biomarkers.