Patent ID: 7704707

Claim:
A biochemical or cellular method of producing an active Streptoverticillium transglutaminase from a Streptoverticillium protransglutaminase comprising contacting a neutral Streptoverticillium mobaraense IFO13819 metalloprotease with the protransglutaminase, wherein said metalloprotease is produced by culturing a microorganism into which the gene encoding the metalloprotease has been introduced, and recovering the active transglutaminase, wherein said metalloprotease comprises: a molecular weight of about 35,000, an optimum pH 6.5 to pH 7.5, an optimum temperature of about 45° C., and an activity to cleave the peptide of SEQ ID NO: 11 wherein, said metalloprotease is strongly inhibited by ethylene diamine tetraacetic acid, 1,10-phenanthroline, phosphoramidon, and Streptomyces subtilisin inhibitor (SSI) from actinomycetes.