Patent ID: 8187824

Claim:
In a method of detecting a target compound, cell or particle by flow cytometry, wherein said target is labeled with a detectable luminescent compound, the improvement comprising utilizing as said detectable luminescent compound a compound comprising a porphyrinic macrocycle; wherein said detectable luminescent compound is excited by light at an excitation wavelength band and detected by emitted light at an emission wavelength band; and wherein said emission wavelength band has a full width at half maximum peak of not more than 30 nanometers: and wherein said porphyrinic macrocycle is: a bacteriochlorin of Formula AI: wherein: M is a metal or is absent; X is selected from the group consisting of Se, NH, CH 2 , O and S; R 1 , R 2 , R 3 , R 4 R 5 , R 6 R 7 and R 8 are each independently selected from the group consisting of H, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkylalkyl, cycloalkylalkenyl, cycloalkylalkynyl, heterocyclo, heterocycloalkyl, heterocycloalkenyl, heterocycloalkynyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, heteroaryl, heteroarylalkyl, heteroarylalkenyl, heteroarylalkynyl, alkoxy, halo, mercapto, azido, cyano, formyl, carboxylic acid, hydroxyl, nitro, acyl, alkylthio, amino, alkylamino, arylalkylamino, disubstituted amino, acylamino, acyloxy, ester, amide, sulfoxyl, sulfonyl, sulfonate, sulfonic acid, sulfonamide, urea, alkoxylacylamino, aminoacyloxy, protein, peptide, antibody, and polyalkylene oxide; subject to the proviso that neither R 1 nor R 2 is H; or neither R 3 nor R 4 is H; or R 1 and R 2 together are ═O; or R 3 and R 4 together form spiroalkyl, or R 3 and R 4 together are ═O; or a bacteriophorbine of Formula DIc or DIc′: wherein: M is a metal or is absent; X i , X 2 , X 3 and X 4 are each independently selected from the group consisting of Se, NH, CH 2 , O and S; R 1 , R 2 , R 3 , R 4 ,R 5 ,R 6 ,R 7 ,R 8 ,R 11 ,R 12 ,R 13 ,R 14 ,R 15 ,R 16 ,R 21 ,R 22 ,R 31 , and R 32 are each independently selected from the group consisting of H, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkylalkyl, cycloalkylalkenyl, cycloalkylalkynyl, heterocyclo, heterocycloalkyl, heterocycloalkenyl, heterocycloalkynyl, aryl, aryloxy, arylalkyl, arylalkenyl, arylalkynyl, heteroaryl, heteroarylalkyl, heteroarylalkenyl, heteroarylalkynyl, alkoxy, halo, mercapto, azido, cyano, acyl, formyl, carboxylic acid, acylamino, ester, amide, hydroxyl, nitro, alkylthio, amino, alkylamino, arylalkylamino, disubstituted amino, acyloxy, sulfoxyl, sulfonyl, sulfonate, sulfonic acid, sulfonamide, urea, alkoxylacylamino, aminoacyloxy, protein, peptide, antibody, and polyalkylene oxide; wherein each pair of R 1 and R 2 , R 11 and R 12 , R 21 and R 22 , or R 31 and R 32 , can together form ═O; wherein each of R 1 and R 2 , R 3 and R 4 , R 11 and R 12 , or R 13 and R 14 , can together form spiroalkyl; wherein R 2 and R 3 can together form a double bond; and wherein R 12 and R 13 can together form a double bond; or a salt thereof.