Patent ID: 8535622

Claim:
A naturally based Sperm Separation System (SSS) for separation of at least a portion of sperm cell populations (SCP) characterized by (i) motility in a range between about 5 μm/s to about 15 μm/s at 37°; (ii) slow progressive motility in a range between about 15 μm/s at 37° to about 25 μm/s at 37°; (iii) rapid progressive motility of at least 25 μm/s at 37° and 20 μm/s at 20°; (iv) at least 90% motile sperm cells, having an average of at least 32% normal morphology; or any combination thereof, within an original semen sample, such that an enriched SCP sample is obtained; said SSS comprising: a. a sperm separation device, comprising: i. at least one first chamber adapted to contain at least a portion of said original semen sample; said first chamber is characterized by pre-determined 3D shape and volume, V; said first chamber is bounded by a rim such that said original semen sample is kept below said rim; wherein said rim is crenellated shaped; and, ii. at least one second chamber in physical communication with said first chamber and said rim; said second chamber is characterized by pre-determined 3D shape and volume, V 1 , wherein said V 1 is substantially smaller than or equal to V; said second chamber is adapted to reside said enriched SCP sample; said enriched SCP sample comprising at least a portion of said SCP crossing through the crenellations in said crenellated shaped rim such that said separation of said SCP from said original semen sample is obtained and said enriched SCP sample is provided; b. incubation means, adapted to socket at least one of said cell separation device and to homogeneously thermoregulate the temperature within the same; wherein said pre-determined 3D shape of said first and second chambers and said crenellations in said rim enables at least one of the following is held true: i. efficacy of said SSS is at least 95%; ii. the total number of motile CSP separated into said second chamber is at least 4*10 6 as examined with phase contrast or light microscopes, when said original semen sample is a NORMAL sample as defined by WHO 1999 Appendix IA clause 2.5 and comprises at least 5*10 6 [motile CSP/ml]; iii. the concentration of motile CSP separated into said second chamber is at least 4*10 6 [cell/ml] as measured with phase contrast or light microscopes when the concentration of motile CSP in said original semen sample is at least 5*10 6 [motile sperm cell/ml]; iv. the total number of progressive motile CSP separated into said second chamber is at least 2*10 6 as measured with phase contrast or light microscopes, when said original semen sample comprises at least 2.5*10 6 [progressive motile CSP/ml]; v. the average percentage of CSP having normal morphology in said enriched sample is at least 32% in as measured with phase contrast or microscopes when said original semen sample comprises an average of at least 18% CSP having normal morphology.