Patent ID: 8246834

Claim:
A method for separating a sample using high pressure flash chromatography, the method comprising the steps of: i) providing a pressurized vessel and a cartridge encapsulated therein, wherein the cartridge defines an adsorption material chamber having a diameter between about 1 cm to 20 cm and containing an adsorption material; ii) pressurizing a compressible fluid, optionally containing a cosolvent, to create a mobile phase; iii) eluting the mobile phase and the sample through the pressurized vessel under high pressure flash chromatography conditions, to obtain a separated sample; iv) heating the mobile phase containing the separated sample after the mobile phase exits the pressurized vessel to facilitate the separation of the compressible fluid; and v) collecting the separated sample, wherein the pressurized vessel contains an adsorption material having a particle size of 10-100 microns, and the pressure of the adsorption vessel is held at 50-350 bar during elution.