Patent ID: 8003759

Claim:
An isolated polypeptide comprising an EF hand domain, wherein the EF hand domain has a structurally significant glutamic acid or aspartic acid residue substituted with a serine or threonine residue, wherein the EF hand domain has the formula: X 1 X 2 DX 3 X 4 X 5 DX 6 WX 7 X 8 X 9 X 10 X 11 X 12 X 13 X 14 X 15 X 16 wherein X 1 is an optionally present amino acid; X 2 is an optionally present amino acid; X 3 is K, A, or L; X 4 is N or D; X 5 is A or G; X 6 is G or R; X 7 is I or R: X 8 is D, R, S, or T; X 9 is A, R, K, P, or F; X 10 is an optionally present A, R, G, or L, provided that, when X 8 is not S or T, X 10 is present; X 11 is an optionally present S, T, or R, provided that, when X 8 is not S or T, X 11 is present and is S or T; X 12 is an optionally present L, P, I, K, or F; X 13 is an optionally present K, A, I, R, G, or L; X 14 is an optionally present A, R, or P; X 15 is an optionally present K; X 16 is an optionally present A.