Patent ID: 7537885

Claim:
A method of preserving a mammalian heart, ex vivo, comprising contacting or perfusing a mammalian heart with an effective amount of an aqueous composition that comprises: a first phase comprising a base nutritive medium; and a second phase comprising nanoparticles that comprise an outer lipophilic coating and an inner hydrophilic core, wherein a) the first phase comprises physiologically compatible concentrations of water soluble or dispersible nutrients, and physiological salts; b) the nanoparticles of the second phase comprise lipids, fatty acids, sterols and free fatty acids; and c) the two-phase composition has an osmolality of at least about 300 mOsM/kg, and wherein the nanoparticles have a mean diameter ranging from about 100 nm to about 300 nm, and wherein the hydrophilic inner core of the nanoparticle contains a solution or suspension comprising a moiety capable of binding and releasing oxygen, and the first phase comprises a composition of the following table: g/Liter RANGE Adenosine 0.950-1.050 Adenosine 5′ Monophosphate 0.0019-0.0021 Adenosine Triphosphate 0.0019-0.0021 Allopurinol 0.133-0.147 B′ Nicotinamide Adenine Dinucleotide 0.038-0.042 Phosphate B′Nicotinamide Adenine Dinucleotide 0.0019-0.0021 Calcium Chloride 0.152-0.168 Choline Chloride 0.0085-0.0094 Chondrotin Sulfate 0.0038-0.0042 Cocarboxylase 0.038-0.042 Coenzyme A 0.0095-0.00105 Cyclodextrin 0.475-0.525 Deoxyadenosine 0.038-0.042 Deoxycytidine 0.038-0.042 Deoxyguanosine 0.038-0.042 Dextran 70 33.25-36.75 Flavin Adenine Dinucleotide 0.038-0.042 Folic Acid 0.0026-0.0028 Glucose 3.800-4.200 Glutathione 0.950-1.050 Glycine 0.0179-0.0197.