Patent ID: 8329433

Claim:
A method for performing a polymerase chain reaction (PCR), comprising: maintaining a reaction mixture in a PCR reaction chamber alternatively at a first temperature and a second temperature using a device comprising: a first heater for heating a temperature-control substance to said first temperature; a second heater for heating said temperature-control substance to said second temperature; a pump located in between and connected in series with said first heater and said second heater; and a bladder unit comprising a pair of bladders, each bladder is coupled to a bladder support and is connected to said first and second heaters via different ports, wherein said pair of bladders are inflatable with said temperature-control substance that controls the temperature of said pair of bladders, wherein said pair of bladders are positioned in a substantially opposing arrangement with a space in between such that both bladders, when inflated, are capable of contacting said reaction chamber placed in said space, and wherein said pump introduces said temperature-control substance into said pair of bladders at said first temperature and said second temperature alternatively with a regular interval to enable the PCR.