Patent ID: 7449295

Claim:
A method of selecting a transfusion donor by determination of compatibility to a recipient by comparing combinations of polymorphic markers in a set of such markers, of candidate donors and a recipient where said determination is performed following subjecting genomic material/DNA from candidate donors and the recipient to amplification to thereby generate amplified products; wherein the amplified products are further subjected to a hybridization assay or to a capture-mediated elongation assay, or both, wherein an assay signal is generated by the individual hybridization or elongation events, as applicable, by pairs of probes wherein pair members are complementary, in whole or in part, to the subsequences of the amplified products which are the same as (and/or which are complementary to) polymorphic markers in the set; and an assay signal intensity pattern is generated from hybridization or elongation events such that each marker in the set is represented by a pair of particular assay signal intensities, the method comprising: determining the assay signal intensity pattern generated by said hybridization or elongation assay and forming, for each probe pair, a combination of signal intensities associated with the members of the pair; generating, from said combinations of signal intensities, a series of values, each value in the series selected from one of three possible values, respectively indicating a state of homozygous-normal, heterozygous or homozygous-variant to thereby form a ternary reaction pattern; determining the polymorphic marker combinations represented by said ternary reaction pattern; and selecting from the candidate donors a donor having said polymorphic marker combination identical to that of the recipient.