Patent ID: 8114973

Claim:
A nucleotide analog of the following Formula II: wherein NTP is a nucleoside or nucleotide triphosphate, or an analog thereof, capable of incorporating onto the 3′ end of a polynucleotide strand hybridized to a template presenting the complement of the NTP; L is a detectable label that facilitates the identification of the nucleotide analog; Inhibitor comprises (a) one or more multiply charged groups or groups capable of becoming multiply charged, or (b) two or more singly charged groups or two or more groups capable of becoming singly charged; R 1 comprises a cleavable bond, which upon cleavage results in de-association of NTP from both L and Inhibitor; R 2 is a tri-valent radical having the formula: wherein R 2 ′ is a tri-valent radical, and R 2 ″ is a bi-valent radical selected from: —(CH 2 ) x —, —(CH 2 —O) x —, —(CH 2 —O) z —(CH 2 ) y —, —(CH 2 ) z —(CH 2 —O) y —, and the same substituted with one or more groups selected from hydroxyl, halogen, amino, thiol, (C 1 -C 6 ) alkyl, wherein x, y and z are each integers with x and y+z are each from 2 to 10; R 3 is a bond or group linking R 2 to the Inhibitor moiety; and R 4 is a bond or group linking R 2 to a L.