Patent ID: 7498136

Claim:
A method for detecting Neisseria in a sample comprising: (a) reacting a mixture comprising: (i) nucleic acid isolated from the sample; (ii) at least a first specific primer capable of being used to amplify nucleic acid of Neisseria gonorrhea , wherein the sequence of the first primer consists of SEQ ID NO: 1; (iii) at least a second specific primer capable of being used to amplify nucleic acid of at least one non-gonococcal species of Neisseria , wherein the sequence of the second primer consists of SEQ ID NO: 2; wherein at least one of the first specific primer and the second specific primer comprise a first non-natural nucleotide base and a label; (iv) at least a universal primer capable of being used to amplify nucleic acid of Neisseria gonorrhea and nucleic acid of the at least one non-gonococcal species of Neisseria; (v) a second non-natural nucleotide base conjugated to a quencher; (b) performing an amplification reaction with the primers of step (a) to produce amplification products of nucleic acid of at least one of Neisseria gonorrhea and the at least one non-gonococcal species of Neisseria , if present, wherein the second non-natural nucleotide base is incorporated opposite the first non-natural nucleotide base; and (c) detecting the amplification products by observing a signal from the label, wherein the signal may be quenched upon incorporation of the second non-natural nucleotide base into the amplification products.