Patent ID: 7432353

Claim:
A fluorescent indicator protein having an increased sensitivity to the concentration of sugars, which is represented by Formula I: wherein: BP (binding protein) is MBP (maltose binding protein) consisting of SEQ ID NO: 30; L 1 and L 2 consist of two amino acids, wherein L 1 is Leu-His and L 2 is Gly-Ser and wherein L 1 is attached to the N-terminal end of BP and L 2 is attached to the C-terminal end of BP; FP 1 is selected from the group consisting of ECFP (enhanced cyan fluorescent protein), EBFP (enhanced blue fluorescent protein) and EGFP (enhanced green fluorescent protein), and FP 2 is selected from the group consisting of EYFP (enhanced yellow fluorescent protein), EGFP (enhanced green fluorescent protein) and RFP (red fluorescent protein).