Patent ID: 8642839

Claim:
A method of transiently expressing a nucleotide sequence of interest in young plant leaf tissue of an intact monocot comprising the steps of: a) producing at least one expression cassette wherein said expression cassette comprises at least one nucleotide sequence operably linked to a promoter; b) using a syringe to agro-infiltrate at least about 0.05 milliliters of a liquid comprising genetically modified Agrobacteria suspended to an OD 600 of 0.5 into at least one visible leaf of a monocot plant wherein the monocot plant is at the two leaf stage or the three leaf stage of development; c) transiently expressing said nucleotide sequence in the said leaf tissue of an intact monocot plant; wherein the monocot plant is selected from the group consisting of maize, wheat, sorghum, barley, millet, oat, sugar cane, and rice.