Patent ID: 8663705

Claim:
A method for using a stable, non-toxic, antimicrobial electrolyzed saline solution exhibiting anti-infective and immune-enhancing potential for use as an in vivo treatment for a human or warm-blooded animal, comprising: a. preparing a saline solution having a saline concentration of at least about 0.15%, b. inserting within the saline solution an inert anode and a spaced apart corresponding inert cathode associated with a power source, c. regulating the temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates and regulate relative concentrations of resulting components during electrolysis, d. circulating the saline solution to maintain a flow of the saline solution between the anode and cathode, and e. applying an effective voltage potential less than about thirty volts between the cathode and the anode sufficient to produce a target mixture of chemically reduced and oxidized species including Hypochlorous acid (HOCl), Hypochlorites (OCl − , NaClO), dissolved Oxygen (O 2 ), Chlorine (Cl 2 ) between 1 to 200 ppm and Hydrogen (H 2 ) gas, Hydrogen Peroxide (H 2 O 2 ), Hydrogen ions (H + ), Hypochloride (ClO) and corresponding amounts of Superoxides (*O 2 − , HO 2 ), Ozone (O 3 ) from 1 to 50 ppm, Activated Hydrogen ions (H − ), Chloride ions (Cl − ), Hydroxides (NaOH, OH − ), Singlet Oxygen (*O 2 ) and other forms of Reactive Oxygen Species (ROS) (*OCl, *HO − ), and wherein total ROS is between about 0.05 and about 50 ppm, utilizing at least one of electron and proton donation, ion and dissolved-gas transport, the temperature, anode and cathode spacing, saline solution circulation rate, and effective voltage combination to achieve desired electrolysis efficiencies and stable specie compositions containing stable ROS compounds while preventing production of chlorates, f. mirroring the target mixture of chemically reduced and oxidized species in the electrolyzed saline solution to the reduced species and reactive oxygen species found in a known biological system by measuring concentrations of reactive oxygen species in the electrolyzed saline solution using a fluorospectrometer and at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein thereby producing the electrolyzed saline solution; and g. administering the electrolyzed saline solution balanced mixture to a human or warm-blooded animal for therapeutic use to attack infective microbes and enhance the ability of the immune system to recognize and destroy damaged or malfunctioning cells.