Patent ID: 7794762

Claim:
A canola protein isolate having a protein content of at least about 90 wt % as determined by Kjeldahl nitrogen×6.25 on a dry weight basis produced by a method which comprises: (a) extracting a canola oil seed meal at a temperature of at least about 5° C. to cause solubilization of protein in said oil seed meal and to form an aqueous protein solution having a protein content of about 5 to about 30 g/L and a pH of about 5 to about 6.8, (b) separating the aqueous protein solution from residual canola oil seed meal, (c) increasing the protein concentration of said aqueous protein solution to at least about 200 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a concentrated protein solution, (d) diluting said concentrated protein solution into water having a temperature below about 15° C. to cause the formation of protein micelles, (e) settling the protein micelles to form an amorphous, sticky, gelatinous, gluten-like micellar mass and a supernatant, (f) separating the protein micellar mass from the supernatant having a protein content of at least about 100 wt % as determined by Kjeldahl nitrogen×6.25 on a dry weight basis, (g) following separating the protein micellar mass therefrom, processing the supernatant to recover additional quantities of canola protein isolate therefrom by: (i) concentrating the supernatant to a protein concentration of about 100 to about 400 g/L, and (ii) drying the concentrated supernatant to provide the additional quantities of canola protein isolate.