Patent ID: 8741557

Claim:
A method of treating a transplant recipient with an increased risk of delayed graft function or an increased risk of graft rejection, the method comprising: (a) obtaining an intra-graft sample from a graft transplanted into the recipient, wherein the sample is obtained from the recipient during the operation in which the graft was transplanted and after a point in time when the graft was transplanted and substantially reperfused; (b) determining a level of expression of one or more genes selected from the group consisting of TNF-α and CD25 in the sample, wherein an increase in the level of expression in the one or more genes relative to a control sample or a reference standard indicates that the transplant recipient has an increased risk of delayed graft function or an increased risk of graft rejection; and (c) administering to the transplant recipient identified as having an increased risk of delayed graft function or an increased risk of graft rejection an immunosuppressant.