Patent ID: 8263339

Claim:
A method for detecting at least one target polynucleotide in a sample comprising: a) contacting a sample containing the at least one polynucleotide with a primer pair that specifically hybridizes to and amplifies a target sequence of the at least one polynucleotide, wherein the primer pair consists of: i) a first primer comprising (1) a 3′ sequence complementary to a first sequence flanking the target sequence of the polynucleotide, and (2) a 5′ capture tag; and ii) a second primer comprising: (1) a 3′ sequence complementary to a second sequence flanking the target sequence of the polynucleotide, and (2) a 5′ sequence that provides a means for directing Abscription; b) amplifying the target sequence from the first and second primers; c) contacting the amplified target sequence with an immobilized molecule that binds the 5′ capture tag, thereby capturing the amplified target sequence of the polynucleotide; d) transcribing at least one Abscript from the means for directing Abscription; and e) detecting at least one Abscript transcribed in step d).