Patent ID: 7604941

Claim:
A method of analyzing nucleic acids comprising: (a) hybridizing one or more copies of a test oligonucleotide comprising at least two informative region sequences to a single molecule of target oligonucleotides under conditions permitting hybridization of the test oligonucleotide to a perfectly complementary sequence; (b) measuring a signal of said hybridization of the test oligonucleotide to the target oligonucleotide, thereby completing a first hybridization trial; (c) dehybridizing the test oligonucleotide; (d) exposing the target oligonucleotide to one or more copies of at least one additional test oligonucleotide, said additional test oligonucleotide comprising at least two informative regions; (e) measuring a signal from the hybridization of the additional test oligonucleotide to the target oligonucleotide, thereby completing a further hybridization trial; (f) repeating steps c) to e) until the information content from the hybridization trials exceeds the information content of the target oligonucleotide; and (g) analyzing said signals.