Patent ID: 7491541

Claim:
A method of assessing wound healing progress comprising: (a) obtaining a tissue sample from a wound site of a subject; (b) processing the tissue sample by a method comprising: (i) removing fat from the tissue sample; (ii) dehydrating the tissue sample; (iii) hydrolyzing the tissue sample to amino acids; and (iv) derivatizating the tissue sample; (c) separating glycine, proline, and hydroxyproline in the processed tissue sample by high-pressure liquid chromatography to obtain analyte peaks areas; (d) determining concentrations of the glycine, proline, and hydroxyproline in the tissue; (e) correlating the concentrations of the glycine, proline, and hydroxyproline in the tissue with an amount of total collagen in the tissue; and (f) comparing the amount of total collagen in the tissue at the wound site with the amount of collagen in non-wounded tissue to assess wound healing progress.