Patent ID: 7795388

Claim:
A circularly permuted chaperonin peptide molecule, comprising a new carboxyl-terminal amino acid and a new amino-terminal amino acid and a reordered chaperonin polypeptide sequence, wherein the circularly permuted chaperonin polypeptide molecule comprises a chaperonin polypeptide molecule which comprises a native carboxyl-terminal amino acid and a native amino-terminal amino acid, wherein the native terminal amino acids are joined directly together or joined via an intervening linker polypeptide sequence, and wherein the chaperonin polypeptide molecule, so joined, is cleaved at a location that differs from the location of the joined native terminal amino acids so as to generate a new carboxyl terminal amino acid and a new amino terminal amino acid and a reordered chaperonin polypeptide sequence, and wherein the chaperonin polypeptide molecule comprises a Sulfolobus shibatae thermophilic factor 55 (TF55) beta polypeptide according to any one of SEQ ID NOS:69, 67, 71, 73, or 75.