Patent ID: 7390674

Claim:
A lateral flow assay device for detecting the presence or quantity of an analyte residing in a test sample, said lateral flow assay device comprising a porous membrane, said porous membrane being in communication with a wicking pad, said porous membrane defining in order: a sample application zone where a sample containing an analyte is deposited; a trigger generation zone in which said analyte reacts with a material to produce hydrogen peroxide; a first detection zone within which is immobilized a first chromophore, said first chromophore being configured to react chemically with hydrogen peroxide to generate a visually detectible signal; a first scavenging zone having a first scavenging material configured to react chemically with hydrogen peroxide without generating a visually detectible signal; a second detection zone within which is immobilized a second chromophore, said second chromophore being configured to react chemically with hydrogen peroxide to generate a visually detectible signal; a second scavenging zone having a second scavenging material configured to react chemically with hydrogen peroxide without generating a visually detectible signal; and, a third detection zone within which is immobilized a third chromophore, said third chromophore being configured to react chemically with hydrogen peroxide to generate a visually detectible signal.