Patent ID: 7459279

Claim:
A method of determining whether a substance is a potential ligand of a neuromedin receptor comprising: (a) contacting cells expressing the receptor chosen from SEQ ID NO:2, SEQ ID NO:4 SEQ ID NO:6 or SEQ ID NO:8, or the polypeptide encoded by a DNA insert contained in the deposit no. CBS 102221 or the deposit no. CBS 102222 at the Centraalbureau voor Schimmelcultures at Baarn the Netherlands with labeled neuromedin U in the presence and in the absence of the substance; or contacting a receptor membrane preparation comprising one of said receptors chosen from SEQ ID NO:2, SEQ ID NO:4 SEQ ID NO:6 or SEQ ID NO:8 or a polypeptide encoded by the DNA insert contained in the deposit no. CBS 102221 or the deposit no. CBS 102222 at the Centraalbureau voor Schimmelcultures at Baarn the Netherlands with labeled neuromedin U in the presence and in the absence of the substance; and (b) measuring the binding of neuromedin U to the receptor.