Patent ID: 7553667

Claim:
A method to regulate expression of a nucleic acid sequence of interest comprising: i) providing a eukaryote having: 1) a first nucleotide sequence comprising, a) said nucleic acid sequence of interest operatively linked to a first regulatory region, b) an operator sequence capable of binding a fusion protein, and; 2) a second nucleotide sequence comprising a second regulatory region in operative association with a nucleotide sequence encoding said fusion protein, said fusion protein comprising, a) a DNA binding protein, or a portion thereof, capable of binding said operator sequence, and; b) a recruitment factor protein, or a portion thereof, capable of binding a chromatin remodeling protein, wherein said recruitment factor protein comprises SEQ ID NO:81 or a fragment thereof, wherein said fragment is capable of binding to HDA19; and ii) growing said eukaryote, wherein expression of said second nucleotide sequence produces said fusion protein that regulates expression of said nucleic acid sequence of interest.