Patent ID: 8778679

Claim:
A method for isolating umbilical cord lining stem cells (ULSCs) from an umbilical cord, said method comprising a) obtaining the lining of an umbilical cord, wherein said lining is substantially free of blood, venous tissue, and arterial tissue; and b) culturing explants of said lining on a fibronectin-coated solid substrate in the presence of a low glucose growth medium for a period of time sufficient for said ULSCs to adhere to said fibronectin-coated solid substrate, said growth medium comprising 15% fetal bovine serum, a stabilized dipeptide of L-alanyl-L-glutamine, antibiotic, and a growth factor selected from the group consisting of basic fibroblast growth factor (bFGF), leukemia inhibitory factor (LIF), and epidermal growth factor (EGF).