Patent ID: 8685702

Claim:
A recombinant cell capable of producing isoprene, the cell comprising: (a) a nucleic acid encoding a first 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) polypeptide of a first species and a nucleic acid encoding a second IspG polypeptide of a second species, wherein the second species differs from the first species; (b) a nucleic acid encoding at least one 1-deoxyxylulose-5-phosphate (DXP) pathway enzyme, and (c) a nucleic acid encoding an isoprene synthase polypeptide, wherein the cell produces isoprene at a greater titer as compared to a cell comprising a nucleic acid encoding an IspG polypeptide of a single species.