Patent ID: 7988902

Claim:
A method for fabricating a one-piece microfluidic package, comprising the steps of: a) selecting a mold cavity with an inlet for injecting a molten polymer wherein the mold cavity has a first side and a second side that is opposite the first side; b) defining a micro pattern of microfluidic channels on the first side of the mold cavity resulting in a plurality of interconnecting and interlocking ports, that include at least one set of an inlet port and an outlet port, the one set including one male port and one female port, so that the ports engage other ports via a male-female friction fit that is leak-free and adhesive free to permit interconnecting and interlocking and snapping together of a plurality of one-piece microfluidic packages in an extended series, and forming an interior microfluidic channel that connects the male port on a first end of the mold cavity to the female port on a second end of the mold cavity, wherein the interior channel has a diameter of less than approximately 1.00 mm; c) defining a plurality of inlet/outlet ports on the second side of the mold cavity, the second side having an inlet/outlet port on a first end of the mold cavity and an inlet/outlet port on a second end of the mold cavity and the plurality of inlet/outlet ports is connected to one of a microfluidic channel and a combination of a microfluidic channel and a fluid reservoir defined on the first side of the mold cavity, and forming a male inlet/outlet port at the first end of the mold cavity sized to connect and interlock with a female inlet/outlet port at the second end of the mold cavity, the first side and the second side together solely providing the one-piece, integrally formed, microfluidic package; d) injecting a molten polymer into the cavity; e) allowing the mold to cool; f) allowing the molten polymer to become a rigid substrate; and g) removing the rigid substrate with a microfluidic channel on a first side and a plurality of inlet/outlet ports on a second side, thereby providing a one-piece, integrally formed, microfluidic package capable of leak-free and adhesive-free interconnecting and interlocking in an extended series.