Patent ID: 7629016

Claim:
A photochemical process using unmodified 1-fluoro-2-nitro-4-azidobenzene for the preparation of an activated polymer surface having an active fluoro group and immobilization of a biomolecule thereon via a covalent bond between the activated polymer surface and the biomolecule, the process comprising the steps of: (a) pouring a solution of unmodified 1-fluoro-2-nitro-4-azidobenzene in an organic solvent onto a polymer surface so as to coat the polymer surface, (b) evaporating the organic solvent at room temperature in the dark so as to thereby form a 1-fluoro-2-nitro-4-azidobenzene coated polymer surface, (c) subjecting the 1-fluoro-2-nitro-4-azidobenzene coated polymer surface of step (b) to photoirradiation for a period ranging from 1 second to 60 minutes, (d) washing the irradiated, coated polymer surface from step (c) with an organic solvent, (e) evaporating any organic solvent remaining after washing the polymer surface from step (d) at ambient temperature to obtain an activated polymer surface having an active fluoro group, (f) placing the biomolecule in contact with the activated polymer surface from step (e), and (g) incubating the activated polymer surface in contact with the biomolecule from step (f) so as to displace the active fluoro group and thereby form the covalent bond between the activated polymer surface and the biomolecule and thereby immobilize the biomolecule on the polymer surface.