Patent ID: 8293225

Claim:
An implant for repair of a cartilaginous defect in a subject, comprising a collagen membrane matrix comprising a mixture of collagen I and collagen III, the membrane having a smooth face and an opposite rough, fibrous face, which is charged with synovial cells prior to implantation, wherein the synovial cells are adhered to the rough, fibrous face of the collagen membrane by passing synovial fluid through said membrane in a device comprising a first chamber and a second chamber, said first and second chambers being separated by said collagen membrane, wherein the synovial fluid is introduced into the first chamber, wherein synovial cells in the fluid are collected on the collagen membrane and wherein the fluid is diffused through the collagen membrane, thereby resulting in a cell-charged collagen membrane, and thereafter waiting an amount of time sufficient to allow adherence of cells on said rough, fibrous face before applying the cell-charged collagen membrane to said defect in said subject.