Patent ID: 7776609

Claim:
A method for using a reference microplate, said method comprising the steps of: placing the reference microplate within an optical interrogation system, wherein said reference microplate includes: an upper plate having a plurality of through-holes which forms sidewalls for a plurality of wells; a lower plate, attached to said upper plate, which forms bottom surfaces for said plurality of wells, wherein said lower plate has a biosensor located on said bottom surface of each well and said biosensor has sensing region extending into each well; and a hardened non-biochemical substance, having a refractive index magnitude within a range of 1.27-1.5, coating said biosensor and at least partially filling one of said wells such that said hardened non-biochemical substance has a depth greater than said sensing region of said biosensor associated with said one of the wells; and interrogating at least one of the biosensors within the reference microplate.