Patent ID: 8017346

Claim:
A method for quantifying G protein-coupled receptor (GPCR)-G protein binding by means of using a cell membrane array, said cell membrane array comprising (i) a support and (ii) cell membrane microdeposits of cell membrane preparations, wherein said support is coated with orthophosphoric acid and wherein said microdeposits are fixed to a support, said method comprising: (i) putting an unlabeled candidate compound in contact with said cell membrane array in the presence of labeled GTP or of a labeled, non-hydrolyzable analog thereof, in conditions allowing the interaction between said compound and said GPCR present in said cell membranes, and between said labeled GTP or analog thereof and said G protein also present in said cell membranes; (ii) washing said membrane array to eliminate said labeled GTP or labeled, non-hydrolyzable analog thereof that is not bound to said G protein; and (iii) quantifying the signal obtained due to the binding of said labeled GTP or labeled, non-hydrolyzable analog thereof to said G protein.