Patent ID: 7122671

Claim:
A chemiluminescent labeling compound for use in the labeling of a substance of biological interest, said compound being capable of undergoing a light emitting reaction in the presence of a dilute aqueous solution of sodium hydroxide and hydrogen peroxide and is defined by the formula: wherein the acridinium moiety is substituted, X − is an anion, R 1 is a substituent selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, and aryl, and R 4 is a substituted phenoxy moiety, with a reactive group capable of reacting with a substance of biological interest being attached, either directly or by means of an alkyl group, to the aryl portion of said phenoxy moiety, said reactive group being selected from the group consisting of: (c) —NCS (d) N + H 2 Y − —C—OR 6 and wherein R 6 has the same definition as R 1 , and Y is a halide, and (e) -azide.