Patent ID: 7502099

Claim:
A method for measuring an optical property of a fluorescent sample by obtaining a total spectral radiance factor of the sample illuminated by an illumination for testing, based on a predetermined bi-spectral radiance factor which is close to a bi-spectral radiance factor of the sample, a spectral intensity of the illumination for testing, spectral intensities of first and second illuminations which are different from each other, and measured spectral intensities of a light emitted from the sample illuminated by said first and second illuminations, said method comprising: calculating a spectral radiance factor by said illumination for testing based on said predetermined bi-spectral radiance factor and said spectral intensity of said illumination for testing; determining a first weight for said first illumination and a second weight for said second illumination so that a calculated spectral radiance factor by a synthesized illumination synthesized by combination of said first and second illuminations weighted by said first weight and said second weight respectively, based on said bi-spectral radiance factor and said spectral intensity of said synthesized illumination matches said spectral radiance factor by said illumination for testing; calculating a spectral intensity of a light emitted from the sample illuminated by said synthesized illumination based on said measured spectral intensities and said first and second weights; and calculating said total spectral radiance factor of the sample illuminated by said illumination for testing based on said spectral intensity of said light emitted from the sample illuminated by said synthesized illumination and a spectral intensity of said synthesized illumination.