Patent ID: 8053246

Claim:
A method of analyzing bio-related molecules comprising immobilizing a bio-related molecule comprising a free saccharide chain, having a reducing end, and an acid group(s) on a solid phase support, having a hydrazide group or aminooxy group, in advance by means of covalent bond formation between the reducing end and the hydrazide group or aminooxy group; reacting the immobilized bio-related molecule comprising a saccharide chain and an acid group(s) with a triazene compound denoted by the following general formula to esterify said acid group(s), wherein the triazene compound is denoted by the general formula wherein X denotes an aryl group, substituted aryl group, hetaryl group, or substituted hetaryl group, and R denotes an alkyl, cycloalkyl, allyl, or benzyl; recovering the bio-related molecule, having an esterified acid group(s), from the solid phase; and then subjecting the recovered bio-related molecule, having an esterified acid group(s), to mass spectrometry.