Patent ID: 7282226

Claim:
A vapor fraction obtained from raw materials, wherein the raw materials comprise seeds of Glycine max (L.) Merr. and at least one material selected from the group consisting of seeds of Sesamum indicum , seeds of Phaseolus radiatus L. and antimicrobial herbal, wherein the vapor fraction is prepared by a process comprising the steps of: (a) providing a crude extract of the raw materials in an alcohol solution containing alcohol at the concentration lower than about 15% wt. or in water; and (b) vaporizing the crude extract at a barometric pressure lower than about 1 atm and at a temperature lower than about 110° C. to obtain the vapor fraction; wherein a spectrogram of the 50 μL of the vapor fraction taken at 200 nm through a high performance liquid chromatography (HPLC) using Waters Sphersorb™-ODS2 column with an inner diameter (I.D.) of 4.6 mm, a length (L) of 250 mm and a particle size of 5 m comprises peaks at a retention time of 2.910, 5.190, 13.190, and 50.815 minutes with the coefficient of variation of 8%, where mobile phase is at 0 to 5 minutes is 95% H 2 O/5% CH 3 CN; at 5 to 30 minutes is 95% H 2 O/5% CH 3 CN in gradient, at 30 to 80 minutes is 75% HO/25% CH 3 CN in gradient; at 80 to 100 minutes is 0% H 2 O/100% CH 3 CN in gradient; at 100 to 101 minutes is 0% H 2 O/100% CH 3 CN in gradient; and at 101 minute is 95% H 2 O/5% CH 3 CN and a flow rate is 1 mL/min.