Patent ID: 7785871

Claim:
A plasmid comprising a polynucleotide sequence comprising the following elements: (a) a homing endonuclease site, (b) a set of rare restriction sites, (c) a region of random nucleotide sequence that can serve as a promoter/intron acceptor module, (d) a set of rare restriction sites; (e) a region of random nucleotide sequence that can serve as an expression acceptor module, (f) a set of rare restriction sites; (g) a region of random nucleotide sequence that can serve as a 3′ regulatory domain acceptor module, (h) a set of rare restriction sites, and (i) a homing endonuclease site in reverse orientation that is identical to the homing endonuclease site in element (a), wherein elements (a) through (i) are arranged sequentially in the 5′ to 3′direction of said plasmid.