Patent ID: 8685649

Claim:
A method of detecting dengue virus in samples containing dengue RNA by LAMP wherein dengue serotypes 1, 2, 3, and 4 are simultaneously detected in a single reaction tube comprising: a. Adding said samples to a reaction tube; b. adding to said reaction tube, LAMP reagents, and outside primers specific for dengue virus; degenerate primers specific for dengue serotypes 1, 2, 3 and 4; and a primer specific for LAMP created loop; c. incubating said reaction tube containing said reagents and primers; d. detecting LAMP-amplified products, wherein said degenerate primers for said serotypes 1, 2, 3 and 4 contain nucleic acid sequences of SEQ ID No. 2 and SEQ ID No. 8, for serotype 2; SEQ ID No. 3 and SEQ ID No. 9, for serotype 1 and 3; and SEQ ID No. 4 and SEQ ID No. 10, for serotype 4 and wherein said outside primers contain nucleic sequences of SEQ ID No. 1; SEQ ID No. 5; SEQ ID No. 6 and SEQ ID No. 7.