Patent ID: 7737325

Claim:
A non-human knock-out mammal lacking alpha-1,6-fucosylation, comprising an exogenous gene encoding an antibody molecule, wherein said mammal produces an antibody composition comprising the antibody molecule, said antibody molecule comprising a Fc region comprising complex N-glycoside-linked sugar chains bound to the Fc region, and said sugar chains comprising a reducing end which contains an N-acetylglucosamine, wherein the sugar chains do not contain fucose bound to the 6-position of the N-acetylglucosamine in the reducing end of the sugar chains, wherein said lack of alpha-1,6-fucosylation results from inactivation of an endogenous gene encoding an enzyme selected from the group consisting of GDP-mannose 4,6-dehydratase (GMD), GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase (Fx), GDP-beta-L-fucose pyrophosphorylase (GFPP) and alpha-1,6-fucosyltransferase (FUT8), such that said non-human knock-out mammal lacking alpha-1,6-fucosylation produces said antibody molecule.