Patent ID: 8105802

Claim:
A biochemical method of producing an active Streptoverticillium transglutaminase from a Streptoverticillium protransglutaminase comprising: A) contacting a neutral Streptoverticillium mobaraense IFO 13819 metalloprotease with the protransglutaminase, and B) recovering the active Streptoverticillium microbial transglutaminase; wherein said metalloprotease comprises: a molecular weight of about 71,000, an optimum pH of 7.0, an optimum temperature of about 55° C., an activity to cleave the peptide of SEQ ID NO: 11; and wherein said metalloprotease is strongly inhibited by ethylene diamine tetraacetic acid, 1,10-phenanthroline, phosphoramidon, dithiothreitol, and Streptomyces subtilisin inhibitor (SSI) from actinomycetes.