Patent ID: 7972858

Claim:
A method for analyzing one or more target polynucleotide, the method comprising: (a) providing a plurality of hybridizable probe monomers, wherein one or more hybridizable probe monomers correspond to a predetermined nucleotide base, and wherein each hybridizable probe monomer is associated with a signal molecule; (b) forming a hybridizable probe polymer by ligating a plurality of hybridizable probe monomers in a sequence corresponding to the nucleotide sequence of a target polynucleotide; (c) annealing the signal molecule associated with each hybridizable probe monomer to the hybridizable probe polymer to form a hybridizable probe polymer-signal molecule complex; and (d) subjecting the complex to analysis by using a nanopore to sequentially remove the signal molecules from the complex, wherein removing the signal molecules from the complex generates a pattern of signals used to identify the sequence of nucleotide bases in said target polynucleotide.