Patent ID: 7250282

Claim:
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 7 or 9, a full-length complement of SEQ ID NO: 7 or 9, and a sequence that is capable of hybridizing to SEQ ID NO: 7 or 9 or to a full-length complement of SEQ ID NO: 7 or 9 under high stringency conditions, wherein the conditions comprise hybridization at 65° C. in 1% BSA, 1 mM EDTA, 0.5 M NaHPO 4 (pH 7.2), 7% SDS, and washing at 68° C. in 0.2 x SSC, 0.1% SDS, and wherein the nucleotide sequence encodes an amino acid sequence that has one or more of the following features: (a) catalyses the conversion of sucrose into isomaltulose and concomitantly produces trehalulose at a yield of less than 5% of the yield of isomaltulose; or (b) catalyses the conversion of sucrose into isomaltulose with a Km of less than 50 mM and with a Vmax of at least 400 μmoles isomaltulose/mg protein/mm; or (c) catalyses the conversion of sucrose into isomaltulose but not the hydrolysis of isomaltulose.