Patent ID: 8318109

Claim:
A microfluidic device for analyzing a liquid sample, the microfluidic device comprising: a first microfluidic channel having a first end and a second end; a sample inlet connected to the first end of the first microfluidic channel, the sample inlet for receiving the liquid sample; a bellows pump connected to the second end of the first microfluidic channel; a second microfluidic channel having a first end and a second end, wherein the first end is connected to the sample inlet, and the second end is connected to the bellows pump; a third microfluidic channel having a first end and a second end, wherein the first end is connected to the sample inlet, and the second end is connected to the bellows pump; a first reagent inlet connected to the first microfluidic channel at a reagent-sample junction downstream from the sample inlet, the first reagent inlet for receiving a first reagent; a second reagent inlet connected to the second microfluidic channel at a reagent-sample junction downstream from the sample inlet, the second reagent inlet for receiving a second reagent; a third reagent inlet connected to the third microfluidic channel at a reagent-sample junction downstream from the sample inlet, the third reagent inlet for receiving a third reagent; and wherein the bellows pump is configured to aspirate the sample and each of the reagents through the reagent-sample junctions and through each of the microfluidic channels toward the bellows pump and to mix each of the reagents with the sample in response to an actuation stroke applied to the bellows pump.