Patent ID: 8895808

Claim:
A method of selecting for unlinked first and second DNA segments in a plant cell, comprising: a. transforming one or more plant cells with a nucleic acid molecule via Rhizobiaceae-mediated transformation, said nucleic acid molecule comprising: a first DNA segment comprising a first transcription unit located between a first left border region and a first right border region; a second DNA segment comprising a second transcription unit located between a second left border region and a second right border region, wherein said border regions are capable of enabling the integration of said segments into a plant genome; and a third transcription unit comprising a non-lethal negative selectable marker gene, wherein said non-lethal negative selectable marker gene is located between said first DNA segment and said second DNA segment, and wherein said left and right border regions are T-DNA border regions; b. growing said transformed one or more plant cells; and c. selecting from said transformed one or more plant cells a plant cell comprising said first DNA segment and said second DNA segment and lacking said non-lethal negative selectable marker gene.