Patent ID: 7476363

Claim:
A microfluicic device for reacting a plurality of different samples with a plurality of different reagents comprising: a) a first layer; b) a second layer; c) a plurality of reaction cells located in said second layer; wherein each reaction cell comprises: i) a first chamber located in the second layer; and ii) a second chamber located in the second layer connected to the first chamber via an interface channel; iii) a first valve in said interface channel between said first chamber and said second chamber that, when actuated, fluidically isolates said first chamber and said second chamber from each other; wherein each said first chamber is in fluid communication with a first fluid channel located in said second layer, each said first fluid channel is in fluid communication with a plurality of first chambers, and each said first fluid channel is in fluid communication with a corresponding fluid inlet through which solution can be introduced into said channel; wherein each said second chamber is in fluid communication with a second fluid channel that traverses an interface between said first and second layers; and wherein a plurality of said second fluid channels are in fluid communication with a corresponding third fluid channel, and each said third fluid channel is located in said first layer and is in fluidic communication with a corresponding fluid inlet through which solution can be introduced into said channel.