Patent ID: 8653241

Claim:
An isolated polypeptide with phospholipase activity selected from a) a polypeptide that is encoded by the coding region of a DNA selected from the group consisting of i) a DNA that comprises the nucleic sequence of SEQ ID NO: 1, ii) a DNA that encodes a protein, wherein said protein has the amino acid sequence of SEQ ID NO: 2, and iii) a full complement of the DNA of (i) and (ii), b) a polypeptide sequence comprising the amino acid sequence of SEQ ID NO: 2 or obtained therefrom, having at least 92% identity to SEQ ID NO:2, c) a polypeptide with a sequence that has at least 92% identity to amino acids 33 to 633 of SEQ ID NO: 2, and d) a polypeptide that is encoded by a nucleic acid sequence that hybridizes under stringent conditions which comprise hybridization at 65° C., 18 h in dextran sulphate solution, washing of the filter for 30 min each, first with 6×SSC, twice 2×SSC, twice 2×SSC, 0.1% SDS and 0.2×SSC at 65° C., with (i) nucleotides 530 to 2388 of SEQ ID NO: 1, (ii) a cDNA sequence comprising nucleotides 530 to 2388 of SEQ ID NO: 1, (iii) a full complementary strand of (i) or (ii).