Patent ID: 8361961

Claim:
An isolated mutant G-CSF polypeptide comprising the wild-type G-CSF sequence SEQ ID NO: 143 wherein the sequence M 1 TPLGP (SEQ ID NO: 190) in SEQ ID NO: 143 is replaced with a mutant peptide sequence, wherein the mutant peptide sequence encodes an O-linked glycosylation site that does not exist in the wild-type G-CSF polypeptide, and wherein the mutant peptide sequence is selected from the group consisting of: MVTPLGP (SEQ ID NO: 1), MQTPLGP (SEQ ID NO: 2), MIATPLGP (SEQ ID NO: 3), MPTQGAMPLGP (SEQ ID NO: 5), MVQTPLGP (SEQ ID NO: 6), MQSTPLGP (SEQ ID NO: 7), MGQTPLGP (SEQ ID NO: 8), MAPTSSSPLGP (SEQ ID NO: 9), MAPTPLGPA (SEQ ID NO: 10), MTPTLGP (SEQ ID NO: 11), MTPTQLGP (SEQ ID NO: 12), MTPTSLGP (SEQ ID NO: 13), MTPTQGP (SEQ ID NO: 14), MTPTSSP (SEQ ID NO: 15), MTPQTP (SEQ ID NO: 16), MTPTGP (SEQ ID NO: 17), MTPLTP (SEQ ID NO: 18), MTPNTGP (SEQ ID NO: 19), MTPVTP (SEQ ID NO: 20), MTPMVTP (SEQ ID NO: 21), and MTPTQGLGPAS (SEQ ID NO: 22).