Patent ID: 7129051

Claim:
A method of correlating celluar morphology and the presence of a cellular target protein with the presence of a cellular target nucleic acid sequence in a section of a tissue sample comprising the following steps: (a) staining the section of tissue sample with a morphological stain; (b) determining cellular morphology in the section of tissue sample; (c) contacting the section of sample tissue with an antibody which specifically binds to the target protein; (d) determining binding of the antibody to the section of tissue sample; (e) hybridizing a fluorescently labeled nucleic acid probe to the target nucleic acid sequence in the same section of tissue sample; (f) detecting the presence of the labeled nucleic acid probe in the section of tissue sample; and (g) correlating step (b) and (d) with step (f), wherein the morphological stain used in step (a) does not significantly autofluoresce at the same wavelength as a fluorescent label of the fluorescently labeled nucleic acid probe used in step (e).