Patent ID: 8551715

Claim:
A method of identifying a cell that produces a heterodimeric molecule of interest comprising: (a) transfecting a cell that expresses a first polypeptide with a polynucleotide that encodes at least one second polypeptide, wherein the first polypeptide comprises (i) an extracellular portion comprising an immunoglobulin heavy chain constant region comprising CH2 and CH3 domains; and (ii) a nonimmunoglobulin transmembrane portion, wherein the first polypeptide comprises a sequence at least 80% identical to SEQ ID NO:10, SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:26, SEO ID NO:28, SEO ID NO:30, SEQ ID NO:31, or SEQ ID NO:32; and wherein the second polypeptide comprises an immunoglobulin heavy chain; (b) incubating the cell to allow expression of a heterodimeric molecule on the surface of the cell, wherein the heterodimeric molecule comprises the first polypeptide and the second polypeptide; (c) contacting the cell with a detection molecule, wherein the detection molecule binds the heterodimeric molecule; and (d) identifying the cell bound by the detection molecule.