Patent ID: 7794939

Claim:
A method of detecting DNA methylation in a biological sample comprising: a) targeting DNA molecules hybridized with hapten-labeled sequence specific probes to produce DNA-hapten-labeled sequence probes; b) capturing the DNA-hapten-labeled sequence probes with a hapten recognizing biomolecule on solid material; c) exposing the captured DNA-hapten-labeled sequence probes to a conjugate of antibody and a DNA template, or a conjugate of antibody and a particle with oligonucleotides; d) generating RNA molecules from the DNA template by an in vitro transcription process, or releasing oligonucleotides from the particle; e) capturing RNA molecules or oligonucleotides by nucleic acid recognizing biomolecules immobilized on a sensing surface of an electronic device through a hybridization process; f) changing the electronic properties of the sensing surface by the presence of RNA molecules or oligonucleotides, thereby generating electronic signals; and g) detecting the generated electronic signals by an electronic signal detector, thereby detecting DNA methylation in the biological sample.