Patent ID: 8509868

Claim:
A method for measuring a concentration of a biogenic substance contained in a living body, the method comprising steps of: a step (a) of preparing a measuring device comprising a light source, a focusing controller, an optical filter, and a light receiver; a step (b) of transmitting a first focused light from the light source through the focusing controller, and focusing the first focused light on a surface of a particle chip implanted in a skin though a first area on the surface of the skin so as to generate a first reflected light, wherein the particle chip comprises a substrate and a plurality of metal particles, a step (c) of receiving the first reflected light by the light receiver through the optical filter to obtain a first signal Xa, the following equation (III) is satisfied: lambda 2 =(10 7 *lambda 1 )/(10 7 −B *lambda 1 ) (III), lambda 2 : the central wavelength of optical filter, lambda 1 : the wavelength of the first focused light, B: Raman shift of the biogenic substance; a step (d) of transmitting a second focused light from the light source through the focusing controller, and irradiating the surface of the particle chip with the second focused light through a second area on the surface of the skin so as to generate a second reflected light, wherein the focal point of the second focused light is different from the focal point of the first focused light, the first area is identical to the second area, and a step (e) of receiving the second reflected light by the light receiver through the optical filter to obtain a second signal Xb, a step (f) of calculating the concentration of the biogenic substance on the basis of the difference between the first signal Xa and the second signal Xb.