Patent ID: 8685687

Claim:
A chimeric recombinase protein comprising a serine recombinase operatively linked to a zinc finger nucleotide binding domain such that the chimeric recombinase protein catalyzes site-specific recombination at a DNA site specifically bound by the zinc finger nucleotide binding domain and, wherein the serine recombinase is a Gin mutein having the following mutation M70V with reference to a wild-type Gin serine recombinase comprising the amino acid sequence as set forth in SEQ ID NO: 707, and one or more of the following mutations: D12G, N14S, N20D, K50E, I94V and M114V with reference to the wild-type Gin serine recombinase, and wherein the zinc finger nucleotide binding domain is selected from the group consisting of a bidactyl zinc finger binding domain that binds a hexanucleotide, a tridactyl zinc finger nucleotide binding domain that binds 9 base pairs, a 4-finger zinc finger nucleotide binding domain that binds 12 base pairs, a 5-finger zinc finger nucleotide binding domain that binds 15 base pairs, and a 6-finger zinc finger nucleotide binding domain that binds 18 base pairs.