Patent ID: 8053201

Claim:
A method of detecting proteins using a microfluidic control chip, comprising: immobilizing anti-immunoglobulin antibodies to a membrane filter of the microfluidic control chip; injecting blood into the chip; binding endogenous antibodies in the blood to the anti-immunoglobulin antibodies while simultaneously separating hemocytes and plasma from the blood with the membrane filter; coupling detection antibodies to fluorescent nano-particles; adsorbing the detection antibodies to a detection antibodies portion of the microfluidic control chip; immobilizing capture antibodies to a capture antibodies portion of the chip; dissolving the detection antibodies into the blood; binding the detection antibodies to the proteins to be detected in the blood; conveying the detection antibodies from the detection antibodies portion of the chip to a capture antibodies portion of the chip; binding the proteins to the capture antibodies; detecting an intensity of fluorescent light emitted from the capture antibodies portion of the chip; and determining a concentration of the proteins in the blood based on the intensity of fluorescent light.