Patent ID: RE39282

Claim:
A method for determining the sensitivity of an HIV- 1 samples to zidovudine, which comprises: (a) isolating HIV- 1 DNA extracted from human cells or HIV- 1 RNA isolated from body fluids, (b) hybridizing a detectably labeled oligonucleotide to the HIV- 1 DNA isolated in step (a), the oligonucleotide having at its 3′ end at least 15 nucleotides complementary to a region of the weld type DNA sequence, its corresponding RNA, to a region of the mutant DNA sequence set forth in FIG. 1, or its corresponding RNA, wherein the oligonucleotide terminates of the 3′-end with said at least 15 nucleotides at the 2328 , 2338 , 2772 , 2773 , or 2784 position, (c) attempting to extend the oligonucleotide at its 3′-end, (d) ascertaining the presence or absence of a detectably labeled extended oligonucleotide, (d) correlating the presence or absence of a detectably labeled extended oligonucleotide in step (d) with the sensitivity of the HIV- 1 samples to zidovudine.