Patent ID: 8466091

Claim:
A focused library of vectors or genetic packages, comprising a first set of variegated DNAs each of which comprises a nucleotide sequence encoding an antibody heavy chain, which comprises a CDR1 region, a CDR2 region, and a CDR3 region, each nucleotide sequence in the first set of variegated DNAs being represented by the formula V::nz::JHn, or V::nz::D::ny::JHn, wherein V is a v gene, nz is a series of bases that are essentially random, D is a D segment, ny is a series of bases that are essentially random, and JHn is one of the six JH segments, wherein the CDR2s of the heavy chains encoded by the first set of variegated DNAs are seventeen amino acids in length, sixteen amino acids in length, and/or nineteen amino acids in length, and wherein the heavy chain CDR2s encoded by the first set of variegated DNAs are seventeen amino acids in length having the sequence: <2>I<2><3>SGG<1>T<1>YADSVKG (SEQ ID NO:2), in which each <1> independently is selected from the group consisting of A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, and Y; each <2> independently is an amino acid selected from the group consisting of Y, R, W, V, G and S; and <3> is selected from the group consisting of P and S.