Patent ID: 8293473

Claim:
A method for the assessment of compatibility of an individual PCR with a defined reference of PCRs comprising the steps of: a) amplifying by PCR a nucleic acid together with a signaling agent in an investigated sample; b) obtaining a reference set by amplifying by PCR a plurality of samples with a reference nucleic acid sequence together with a signaling agent; c) obtaining a time series of signals from every PCR by reading and storing at a plurality of time points at least one signal whose intensity is related to the quantity of the nucleic acid sequence formed in the PCR; d) from the time series of signals of every PCR, deriving a plurality of parameters characterizing change of the signal accompanying the reaction progression; e) simultaneously comparing by a single statistical test the parameters of the investigated PCR with the parameters of the PCRs of the reference set; f) based on the probability obtained from the statistical test, drawing an inference on the geometry of the reaction kinetics of the investigated PCR with the reference set, wherein a parallel geometry indicates reaction kinetics compatible between the investigated PCR and the reference set; and g) informing a user about one or more PCRs with non-parallel geometry if this is elicited in step f.