Patent ID: 7749722

Claim:
A method for measuring an analyte present in plasma of a whole blood sample comprising the steps of: (1) bringing the whole blood sample into contact with a first substance which is immobilized on an insoluble carrier and which specifically binds to the analyte to be measured and a second substance which is labeled with alkaline phosphatase and which specifically binds to the analyte to be measured, under conditions where hemolysis is substantially avoided, to form a complex comprising first substance-analyte-second substance on the insoluble carrier, (2) washing the insoluble carrier with a washing liquid to remove unbound second substance, and (3) measuring said complex on the basis of an enzyme reaction of the alkaline phosphatase with an alkaline phosphatase substrate with the addition of an inhibitor of endogenous alkaline phosphatase, the measuring step (3) being carried out in the presence of the inhibitor of endogenous alkaline phosphatase.