Patent ID: 8394602

Claim:
A method for measurement of an endotoxin level in a sample, comprising: reacting sample, a reagent, and a synthetic luminescent substrate, wherein the reagent contains Factor C, optionally Factor B and optionally a coagulating enzyme, wherein the synthetic luminescent substrate comprises a luminescent substrate bound to a peptide, wherein endotoxin present in the sample binds to the Factor C to form activated Factor C, wherein the activated Factor C binds to the Factor B if present to form activated Factor B, wherein the activated Factor B if present binds to the coagulating enzyme if present to form activated coagulating enzyme, wherein the bond between the luminescent substrate and the peptide of the synthetic luminescent substrate is cleaved by any one of the activated Factor C, the activated Factor B or the activated coagulating enzyme to release the luminescent substrate from the synthetic luminescent substrate, reacting a luminescent enzyme with the released luminescent substrate to generate luminescence, measuring the luminescence intensity, and quantifying the level of endotoxin in the sample based on the measured luminescent intensity.