Patent ID: 8420348

Claim:
A monoclonal rabbit antibody, or antigen-binding fragment thereof, against integrin with insect-derived glycosylation pattern and/or integrin with another eukaryotic glycosylation pattern, each comprising at least a light chain variable region (VL) and a heavy chain variable region (VH), wherein the antibody has antigen binding specificity for an exposed epitope of an extracellular integrin domain or extracellular integrin chain domain, and wherein the antibody is able to bind to intact heterodimers of integrin in formalin fixed paraffin embedded (FFPE) material and on viable cells with the substantially same specificity wherein said antibody is selected from the group consisting of: an antibody that binds to the extracellular domain of integrin αvβ3 and V L comprises the amino acid sequence of SEQ ID NO: 95 (V L -αvβ3) and V H comprises the amino acid sequence of SEQ ID NO: 96 (V H -αvβ3); an antibody that binds to the extracellular domain of integrin αvβ5 and V L comprises the amino acid sequence of SEQ ID NO: 15 (V L -αvβ5) and V H comprises the amino acid sequence of SEQ ID NO: 16 (V H -αvβ5); an antibody that binds to the extracellular domain of integrin αvβ6 and V L comprises the amino acid sequence of SEQ ID NO: 135 (V L -αvβ6) and V H comprises the amino acid sequence of SEQ ID NO: 136 (V H -αvβ6); an antibody that binds to the extracellular domain of integrin αvβ8 and V L comprises the amino acid sequence of SEQ ID NO: 175 (V L -αvβ8) and V H comprises the amino acid sequence of SEQ ID NO: 176 (V H -αvβ8); and an antibody that binds to the extracellular domain of integrin chain αv and V L comprises the amino acid sequence of SEQ ID NO: 215 (V L -αv) and V H comprises the amino acid sequence of SEQ ID NO: 216 (V H -αv).