Patent ID: 8859468

Claim:
A method for identifying cervical cancer in a human, comprising: a) obtaining nucleic acid from a test sample from the human; b) performing bisulfite modification to the nucleic acid in a); c) performing quantitative real-time methylation specific PCR (QMSP) on bisulfite modified nucleic acid from b) using PCR primers and probes specific for a promoter region of one or more genes of interest, wherein the one or more genes of interest are selected from the group consisting of GGTLA4, FKBP6, ZNF516, SAP130, and INTS1, and the primers and probes are selected from the group consisting of SEQ ID NOS: 23-42; d) determining a promoter methylation level of the promoter regions of the one or more genes of interest in the nucleic acid from the test sample of the human; e) providing a reference non-neoplastic test sample; f) comparing the level of promoter methylation of the one or more genes of interest from the test sample of the human, to the level of promoter methylation of the one or more genes of interest in a reference non-neoplastic test sample; and g) identifying said human as having cervical cancer when the level of promoter methylation of the one or more genes of interest in the test sample of the human, is increased relative to the level of promoter methylation of the one or more genes of interest in a reference non-neoplastic test sample indicating epigenetic silencing of the one or more genes of interest.