Patent ID: 8273870

Claim:
A double-stranded ribonucleic acid (dsRNA) for selective inhibition of expression of a mutant target gene, the dsRNA comprising: a first strand that has a complementary region that is complementary to at least a portion of an RNA transcript of at least part of the mutant target gene; and a second strand of the dsRNA, wherein the first and second strands are complementary or substantially complementary to each other, wherein the first and second strands of the dsRNA are 19 to less than 30 nucleotides in length, wherein the complementary region of the first strand of the dsRNA contains a mismatch relative to the mutant target gene and at least one more mismatch relative to a wild type form of the mutant target gene, and wherein the dsRNA selectively inhibits expression of the mutant target gene in a mammalian cell relative to the wild-type form of the mutant target gene.