Patent ID: 7074583

Claim:
A method for identifying phosphoprotein from sample proteins, which comprises the steps of: (i) solubilizing the sample proteins; (ii) adding a preservations buffer to the sample proteins solution to prevent precipitation of the proteins and deactivation of phosphatase; (iii) dividing the sample proteins solution into two parts, sample â€œaâ€ and sample â€œbâ€; (iv) dephosphorylating the sample â€œbâ€ with phosphatase; (v) subjecting the sample â€œaâ€ and the sample â€œbâ€ to two-dimensional electrophoresis under same conditions, thereby spotting each sample protein on electrophoresis gel â€œaâ€ and electrophoresis gel â€œbâ€, respectively; (vi) comparing protein spots on the electrophoresis gel â€œbâ€ with those on the electrophoresis gel â€œaâ€; and (vii) identifying the protein of which spot position on the electrophoresis gel â€œbâ€ is migrated to the alkaline side of the isoelectric focusing compared with the corresponding spot on the electrophoresis gel â€œaâ€ as the phosphoprotein.