Patent ID: 7285381

Claim:
A method for purifying AAV particles, said method comprising providing AAV particles having a structural protein of adeno-associated virus (AAV) that comprises at least one insertion in VP3 located directly adjacent to an amino acid in the sequence selected from the group consisting of YKQIS SQSGA (SEQ ID NO: 2), YLTLN NGSQA (SEQ ID NO: 3), YYLSR TNTPS (SEQ ID NO: 4), EEKFF PQSGV (SEQ ID NO: 5), NPVAT EQYGS (SEQ ID NO: 14), LQRGN RQAAT (SEQ ID NO: 8), and NVDFT VDTNG (SEQ ID NO: 9), wherein said insertion brings about an alteration in the chromatographic properties of the virus; and purifying said AAV particles.