Patent ID: 8632243

Claim:
A method of mixing different species in microfluidic-volumes of fluids, comprising the steps of: adding at least a first and second fluids, the first fluid contains at least a first species and the second fluid contains at least a second species, in an amount of from about 1-100 μL to a cavity in a rotating platform; rotating said rotating platform by varying the angular speed over time to generate circulatory flow, by continuous acceleration and deceleration, in said cavity for mixing said first and second species containing in said first and second fluids; if said first and second species are not sufficient diffused, then re-rotating said rotating platform by varying the angular speed over time to generate circulatory flow, by continuous acceleration and deceleration, in said cavity for mixing the first and second species in said first and second fluids; and determining whether the first and second species are sufficiently diffused after said mixing, wherein said cavity is closed, preventing the first and second fluids from entering and leaving said cavity, while the rotating platform is rotating.