Patent ID: 8198046

Claim:
A fusion DNA construct encoding a fusion polypeptide comprising in operable linkage from the 5′end of said construct, a promoter, a first DNA molecule, which is different from any one of the second, the third, and the fourth DNA molecules, encoding a signal sequence, a second DNA molecule, which is different from any one of the first, the third, and the fourth DNA molecules, encoding a carrier protein, a third DNA molecule, which is different from any one of the first, the second, and the fourth DNA molecules, encoding a KEX2 region, said region comprising a KEX2 site (B 1 B 2 ) and a KEX2 site pre-sequence immediately 5′ to the KEX2 site, wherein the KEX2 region is a sequence selected from VAVEKR (SEQ ID NO:9), VAVYKR (SEQ ID NO:34), LAVEKR (SEQ ID NO:39), KAVEKR (SEQ ID NO:41), VAIEKR (SEQ ID NO:74), VALEKR (SEQ ID NO:75), VAFEKR (SEQ ID NO:72), VWVEKR (SEQ ID NO:67); VEVEKR (SEQ ID NO:55), or VPVEKR (SEQ ID NO:62); and a fourth DNA molecule, which is different from any one of the first, the second, and the third DNA molecules, encoding a desired protein.