Patent ID: 8906308

Claim:
A hematology analyzer comprising: a) a flow cell; b) a light source for directing light to said flow cell; c) a plurality of detectors for detecting a plurality of optical data from a blood sample passing through said flow cell, wherein the plurality of detectors comprises one or more of: an axial light loss (ALL) detector, an intermediate angle scatter (IAS) detector, a depolarized side scatter (DSS) detector, a polarized side scatter (PSS) detector, a green fluorescence (FL 1 ) detector, a yellow to orange fluorescence (FL 2 ) detector, and a red fluorescence (FL 3 ) detector; d) a colorimetric transducer for measuring hemoglobin concentration in the blood sample; e) an impedance transducer for measuring changes in impedance in the blood sample; and f) a data analysis workstation comprising a processor, wherein the processor comprises a non-transient, computer-readable medium programmed with instructions that, when executed by the processor, cause the hematology analyzer to: i) pass the blood sample through the flow cell and direct the light source to the flow cell to generate a plurality of optical data using the plurality of detectors; ii) measure a hemoglobin concentration in the blood sample using the colorimetric transducer; iii) measure a change in impedance in the blood sample using the impedance transducer; and iv) analyze the optical data, the hemoglobin concentration, and the change in impedance to calculate the volume and/or hemoglobin content of an individual red blood cell in the blood sample, wherein the volume of an individual red blood cell is calculated using a second order global volume model that comprises an IAS measurement and an ALL measurement of the individual red blood cell, and wherein the hemoglobin concentration of an individual red blood cell is calculated using a local fit cellular hemoglobin concentration model that comprises an IAS measurement and a PSS measurement of the individual red blood cell.