Patent ID: 7550584

Claim:
A method for purifying previously isolated soluble beta-1,3/1,6-D-glucan of low molecular weight, comprising: a) suspending dry weight beta-1,3/1,6-D-glucan in a first volume of water and an effective amount of carbohydrase enzymes while heating at 10-80° C. and stirring; b) collecting beta-1,3/1,6-D-glucan and pumping it through a first filter forming an ultrafiltrate; c) dialyzing the ultrafiltrate by pumping the ultrafiltrate through dialysis fibers with a molecular weight cut-off of about 1500 daltons; d) concentrating the ultrafiltrate in alcohol; and e) purifying the ultrafiltrate, thereby purifying soluble beta-1,3/1,6-D-glucan having a molecular weight of about 12,000 to 60,000 daltons and containing about 4% protein and >10% β(1→6) bonds.