Patent ID: 8758592

Claim:
A method for measuring a blood sample, comprising: providing: a reaction chamber and a detection chamber formed in a separator disposed between two electrodes; a fill chamber at least partially formed in the separator and one of the two electrodes, spaced a distance apart from the detection chamber, and overlapping at least a portion of the reaction chamber; and a vent at least partially formed in the separator and the two electrodes, spaced a distance apart from the reaction chamber, and overlapping at least a portion of the detection chamber; providing: an antibody-enzyme conjugate in a first buffer and magnetic beads linked to an antigen in a second buffer in the reaction chamber; ferricyanide, glucose, and a mediator in a dilute acid in the detection chamber; a first seal over a first side of the vent by way of a hydrophilic adhesive tape, wherein the hydrophilic adhesive tape also forms a wall of the fill chamber; and a second seal over a second side of the vent by way of a sealing component; providing a blood sample to the fill chamber, wherein at least a portion of the blood sample moves from the fill chamber to the reaction chamber; opening the vent after a pre-determined time by piercing at least one of the hydrophilic adhesive tape and the sealing component, thereby allowing portions of the blood sample containing the antibody-enzyme conjugate that is not bound to the magnetic beads to move to the detection chamber; catalyzing oxidation of the glucose in the detection chamber, which results in the formation of ferrocyanide; applying a potential between the two electrodes; electrochemically detecting a current from the ferrocyanide; and calculating a concentration of the antigen in the blood sample based on the signal detected.