Patent ID: 7820794

Claim:
A manufacturing method of a long-lasting collagen, comprising the steps of: a) scraping extra muscle and fat tissues from a pig skin, and cutting the remaining portion of the pig skin into small tissue segment; b) dipping the small tissue segment into an organic solvent, and rinsing the small segment tissues until grease is removed completely; c) dipping the degreased small tissue segment in a salt water at a first predetermined temperature for a first predetermined time, and then in an acidic solution with a specific pH value for the first predetermined time to inhibit the small segment tissues; d) dipping the inhibited small tissue segment in a first solution at the first predetermined temperature for the first predetermined time to digest the small tissue segment into a second solution; e) perform a centrifugal separation of the second solution at a first predetermined condition to separate the digested small tissue segment from the second solution; f) adding a salt water solution into the separated second solution to prepare a third solution, while shaking the solution severely until a cloudy substance is formed; g) performing a centrifugal separation to the shaken third solution in a second predetermined condition while collecting a lower-layer precipitate, and putting the lower-layer precipitate into water while adding sodium hydroxide to adjust a pH value to form a fourth solution; h) freezing the fourth solution at a second predetermined temperature for the first predetermined time, and then drying the fourth solution to obtain a collagen; i) preparing the collagen into a collagen solution, and mixing the collagen solution with γ-polyglutamic acid (γ-PGA), both having a first predetermind quantity, into a fifth solution; j) titrating a second predetermined quantity of glutaraldehyde solution in the fifth solution by a pump, while blending the collagen of the fifth solution and the glutaraldehyde solution at a predetermined rotating speed for a second predetermined time to perform a first crosslinking; and k) titrating the second predetermined quantity of the glutaraldehyde solution in the fifth solution by the pump again, while blending at the predetermined rotating speed for the second predetermined time to perform a second crosslinking to obtain the long-lasting collagen.