Patent ID: 8338575

Claim:
A method of preparing a fusion protein comprising: expressing in a host cell a nucleic acid encoding a fusion protein comprising a first portion comprising an Fc region from IgG1 or IgG2, wherein the Fc region comprises a hinge region, a CH2 domain, and a CH3 domain, and a second portion comprising a polypeptide having an amino acid sequence comprising at least 95% sequence identity with wild-type mature human interleukin-7 (IL-7), wherein wild-type human IL-7 consists of amino acid residues 26-177 of SEQ ID NO:1; and harvesting the fusion protein, wherein the amino acid residues corresponding to positions 70 and 91 of wild-type mature human IL-7 are glycosylated and the amino acid residue corresponding to position 116 of IL-7 is non-glycosylated.