Patent ID: 8143027

Claim:
A method for the preparation of a chimeric plasminogen activator exhibiting plasminogen activation, said method comprising: (a) preparing a recombinant host cell comprising a nucleic acid molecule comprising the polynucleotide sequence of SEQ ID NO: 9, (b) culturing the recombinant host cell of (a) under conditions suitable to produce the chimeric polypeptide encoded by said nucleic acid molecule, (c) isolating extracellular fluid from fermentation or intracellular milieu of the host cell or combinations thereof from the culture of (b), and purifying the chimeric polypeptide, (d) refolding the purified chimeric polypeptide of (c) by oxidation, and (e) further purifying the refolded chimeric polypeptide of step (d), wherein the purification steps of (c) and (e) are carried out by conventional methods of protein separation such as ion exchange, hydrophobic interaction and molecular sieve chromatography.