Patent ID: 8603807

Claim:
A vector comprising each of the following elements arranged sequentially to form a module open reading frame, said vector comprising a) a first restriction site that codes for a first group of amino acids, wherein the first group of amino acids comprises at least two amino acids; b) a first open reading frame coding for a polypeptide of interest, wherein the coding sequence of the polypeptide of interest is in frame with the coding sequence of at least two amino acids of the first restriction site; c) a second restriction site that codes for a second group of amino acids, wherein the second group of amino acids comprises at least two amino acids, wherein the coding sequence of the second group of amino acids of the second restriction site is in frame with the coding sequence of the polypeptide of interest of the first open reading frame, and wherein the polynucleotide sequence of the second restriction site is not the same as the polynucleotide sequence of the first restriction site; d) a spacer polynucleotide sequence, wherein the spacer polynucleotide sequence codes for at least three spacer amino acids, and wherein the coding sequence for the spacer sequence is in frame with the polynucleotide sequence of the second restriction site; and e) a third restriction site that codes for a third group of amino acids, wherein the third group of amino acids comprises at least two amino acids, wherein the coding sequence of the third group of amino acids of the third restriction site are in frame with the coding sequence of the at least three spacer amino acids and wherein the polynucleotide sequence of the third restriction site is not the same as the polynucleotide sequence of the first restriction site and the third restriction site is not the same as the polynucleotide sequence of the second restriction site, wherein elements (a) through (e) are in-frame linked to one another to form the module open reading frame, wherein the first restriction site is susceptible to a restriction enzyme that would produce a first single-stranded overhang comprising at least 2 nucleotides, wherein the second restriction site is susceptible to a restriction enzyme that would produce a second single-stranded overhang comprising at least 2 nucleotides, wherein said first and second overhangs are complementary to one another, whereupon the annealing of the first and second overhangs, the first restriction site and the second restriction site are destroyed, and wherein the third restriction site is susceptible to a restriction enzyme that would not produce an overhang compatible with the overhang resulting from the restriction of the first restriction site or the overhang resulting from the restriction of second restriction site.