Patent ID: 8637290

Claim:
An isolated, synthetic or recombinant polypeptide having a phospholipase activity, and: (a) comprising an amino acid sequence (i) having at least 95% sequence identity to SEQ ID NO:176, and having at least one of the amino acid mutations of E41A, E41W, E41F, E41Y, or E41R, or equivalent amino acid mutations, wherein optionally the sequence identities are determined by analysis with a sequence comparison algorithm or by a visual inspection; or, (ii) encoded by a nucleic acid (polynucleotide) sequence having at least 95% sequence identity to SEQ ID NO:177, and having at least one modification encoding E41A, E41W, E41F, E41Y, or E41R, or equivalent amino acid mutations; (b) comprising the polypeptide of (a) but lacking a signal sequence or a proprotein sequence; (c) further comprising a heterologous amino acid sequence; (d) comprising the polypeptide of (c), wherein the heterologous amino acid sequence comprises, or consists of a heterologous (leader) signal sequence, or a tag or an epitope; (e) comprising the polypeptide of any one of (a) to (d), wherein the polypeptide is glycosylated, or the polypeptide comprises at least one glycosylation site, (ii) the polypeptide of (i) wherein the glycosylation is an N-linked glycosylation or an O-linked glycosylation; (iii) the polypeptide of (i) or (ii) wherein the polypeptide is glycosylated after being expressed in a yeast cell; or (iv) the polypeptide of (iii) wherein the yeast cell is a P. pastoris or a S. pombe; (f) the polypeptide of any one of (a) to (e), wherein (i) the polypeptide further comprises additional amino acid residues between a signal sequence (leader sequence or leader peptide) and the enzyme, or (ii) the polypeptide of (i), wherein the additional amino acid residues comprise Glu-Ala.