Patent ID: 7919239

Claim:
A method comprising: a) contacting a sample comprising a plurality of labeled miRNA molecules with an array of probes, each of which probes is: i. bound to a substrate ii. complementary to said labeled miRNA molecules starting at the 3′ terminal nucleotide of said labeled miRNA molecules, to produce a plurality of duplexes between said probes and said labeled miRNA molecules, wherein said plurality of duplexes comprises: first duplexes in which the 5′ end of said labeled miRNA molecules is hybridized with said probe and second duplexes in which the 5′ end of said labeled miRNA molecules is not hybridized with said probe, and wherein the melting temperatures of said duplexes are within 15° C. of each other; and b) detecting binding of said miRNA molecules with each of said probes.