Patent ID: 8187842

Claim:
A bacterium comprising a first recombinant nucleic acid integrated into its genome, said first recombinant nucleic acid having an isocitrate lyase coding sequence of an E. coli aceBAK operon operably associated with a first non-native promoter that provides a means for the bacterium to overexpress the isocitrate lyase coding sequence and wherein the bacterium simultaneously overexpresses at least one of a malate synthase G coding sequence from a glcB gene and a malate synthase A coding sequence from the aceB operon, wherein when the glcB gene is over expressed the glcB gene is expressed from a second recombinant nucleic acid integrated into the genome and said glcB gene is operably associated with a second non-native promoter that provides means for overexpression of the malate synthase G coding sequence of the glcB gene simultaneously with overexpression of the isocitrate lyase coding sequence from the aceBAK operon.