Patent ID: 7566528

Claim:
A method for identifying inhibitors of a receptor tyrosine kinase activity comprising the steps of: A) providing S. cerevisiae host cells comprising a nucleic acid construct encoding a peptide which comprises: i) a myristolation signal for membrane anchoring, and ii) a heterologous dimerization domain or a functional fragment thereof, and iii) a tyrosine kinase domain of a receptor tyrosine kinase or a functional fragment thereof, wherein said peptide lacks an extracellular domain and a transmembrane domain, and wherein said host cells harbor a mutation in a ABC transporter gene; B) contacting said host cells with a candidate compound, and C) identifying inhibitors of said receptor tyrosine kinase activity by cultivation of said host cells under suitable conditions such that the inhibition of the receptor tyrosine kinase activity by the candidate compound leads to cell proliferation.