Patent ID: 8592150

Claim:
A method of obtaining sequence information from a genome, said method comprising: (a) providing a population of first fragments of said genome; (b) preparing emulsion droplets of said first fragments, such that each emulsion droplet comprises a subset of said population of first fragments; (c) fragmenting said first fragments, thereby obtaining a population of second fragments within each emulsion droplet, such that said second fragments are shorter than said first fragments; (d) combining individual emulsion droplets comprising said second fragments with individual emulsion droplets comprising adaptor tags or adaptor tag combinations, thereby forming fused droplets; (e) ligating said second fragments with said adaptor tags or adaptor tag combinations within the fused droplets to form tagged fragments; (f) combining the fused droplets to produce a mixture containing tagged fragments; (g) obtaining sequence reads from tagged fragments in the mixture; (h) assembling the sequence reads to produce assembled sequence information for the genome, wherein the assembled sequence information comprises heterozygous loci; and (i) phasing the heterozygous loci using sequence information from the adaptor tags.