Patent ID: 8722326

Claim:
A method of identifying a nucleotide sequence of a target nucleic acid, said method comprising: (a) providing a plurality of constructs disposed on a surface of a substrate, wherein each of said plurality of constructs comprises multiple copies of a monomer and each monomer comprises a first adaptor, a second adaptor, and fragments of the target nucleic acid and has been formed by a process that comprises: (i) forming a first circular DNA comprising a fragment of said target nucleic acid and a first adaptor, wherein the first adaptor comprises a binding site for a restriction enzyme that cleaves DNA at a cleavage site separated from said binding site by at least six nucleotides; (ii) forming a linearized DNA by a process that comprises cleaving the first circular DNA at a site that is internal to the fragment of the target nucleic acid using a restriction enzyme that binds to said binding site; and (iii) forming a second circular DNA comprising said linearized DNA and a second adaptor thereby forming said monomer; then (b) identifying a nucleotide sequence on either side of the second adaptor in a plurality of the constructs in the array.