Patent ID: 7186539

Claim:
A method for refolding recombinant Beta-site APP cleaving enzyme (BACE) polypeptide comprising: a) solubilizing a recombinant BACE polypeptide in a denaturant at a pH of about 10–11 and in the presence of a reducing agent; b) diluting the solubilized BACE polypeptide in an aqueous solution having a temperature of about 1° C. to 15° C., to obtain a diluted sample; and c) incubating the diluted sample at a temperature of about 4° C. to 15° C. until the recombinant BACE polypeptide folds into an active enzyme, wherein the recombinant BACE lacks a leader sequence and at least the first 24 amino acids of the BACE prosequence.