Patent ID: 7709238

Claim:
A method of removing an enzyme from a liquid enzyme reaction mixture, comprising: a. providing the liquid enzyme reaction mixture, wherein the liquid enzyme reaction mixture is produced by: i. a phospholipid base exchange reaction comprising reacting, in the presence of an enzyme, a phospholipid with a compound having an alcoholic hydroxyl group, wherein the enzyme is capable of transferring a phosphatidyl group of the phospholipid to the compound, and wherein the compound having an alcoholic hydroxyl group is selected from the group consisting of an alcohol, a saccharide, and a cyclic compound having a hydroxyl group; or ii. a phospholipid hydrolysis reaction comprising enzymatically hydrolyzing a phospholipid in the presence of water; and optionally adding a protease to the phospholipid base exchange reaction (i) or the phospholipid hydrolysis reaction (ii) to deactivate the enzyme; b. adding to the liquid enzyme reaction mixture an organic solvent; at least one inorganic metal salt selected from the group consisting of sodium salt, potassium salt, calcium salt, magnesium salt, and ammonium salt; and optionally water, to remove the enzyme and/or the protease and to wash the liquid enzyme reaction mixture, thereby removing the enzyme from the liquid enzyme reaction mixture; wherein the enzyme is selected from the group consisting of lipase, phospholipase A 2 (PLA 2 ), and phospholipase D (PLD).