Patent ID: 8691226

Claim:
A humanized monoclonal antibody, comprising: a light chain, wherein the amino acid sequence of a variable region of the light chain comprises SEQ ID. NO.: 1; and a heavy chain, wherein the amino acid sequence of a variable region of the heavy chain comprises SEQ ID. NO.: 2, wherein SEQ ID. NO.: 1 and SEQ ID. NO.: 2 have at least one amino acid substitution, and the amino acid substitution is selected from a group consisting of isoleucine at position 10 of SEQ ID. NO.: 1, being substituted with threonine, lysine at position 18 of SEQ ID. NO.: 1, being substituted with arginine, lysine at position 2 of SEQ ID. NO.: 2, being substituted with glutamine, tryptophan at position 10 of SEQ ID. NO.: 2, being substituted with leucine, lysine at position 18 of SEQ ID. NO.: 2, being substituted with arginine and glutamic acid at position 41 of SEQ ID. NO.: 2, being substituted with glycine, and wherein the humanized monoclonal antibody is capable of binding to a tumor necrosis factor-alpha.