Patent ID: 8198089

Claim:
A method for producing a Kluyveromyces marxianus transformant having a flocculation property and thermotolerance comprising the following steps (A) to (C) sequentially: (A) a step of producing Saccharomyces cerevisiae transformant by introducing a marker gene sequence and an expression promoter sequence in the upstream of at least one FLO gene selected from endogenous FLO1 gene and endogenous FLO9 gene of Saccharomyces cerevisiae; (B) a step of obtaining a DNA fragment containing the marker gene sequence, expression promoter sequence, and FLO gene sequence from a chromosomal DNA derived from the Saccharomyces cerevisiae transformant produced in step (A); and (C) a step of producing a Kluyveromyces marxianus transformant by introducing the DNA fragment obtained in step (B) into Kluyveromyces marxianus as a FLO gene expression cassette.