Patent ID: 8114845

Claim:
A method of increasing a T cell response in a human, comprising administering to said human a fusion protein comprising first and second peptide portions wherein said first peptide portion consists of an amino acid sequence selected from: wild type B7-DC, an amino acid sequence having at least 98% identity to amino acids 20-221 or 20-121 of SEQ ID NO: 1 and which competes in vitro with wild-type B7-DC for binding to PD-1, a fragment of B7-DC which competes in vitro with wild-type B7-DC for binding to PD-1, and an extracellular domain of B7-DC and said second peptide portion comprises a portion of an immunoglobulin (Ig) and a potentiating agent selected from the group of: cyclophosphamide, an analog of cyclophosphamide, sunitinib, anti-TGFβ, imatinib, anthracyclines, oxaliplatin, doxorubicin wherein said potentiating agent is administered before said fusion protein and said fusion protein is administered without said potentiating agent following administration of said potentiating agent.