Patent ID: 7056710

Claim:
A method for producing ML-236B comprising: (a) culturing a Penicillium host cell having been transformed by a vector comprising a polynucleotide sequence encoding mlcR, wherein said vector does not comprise a polynucleotide sequence encoding at least one of mlcA, mlcB, mlcC, and mlcD and (b) recovering ML-236B from the resultant culture; wherein said Penicillium host cell is selected from the group consisting of Penicillium citriurm, Penicillium brevicompactum and Penicillium cyclopium; and wherein mlcR has the amino acid sequence of SEQ ID NO: 42, mlcA has the amino acid sequence of SEQ ID NO: 44, mlcB has the amino acid sequence of SEQ ID NO: 46, mlcC has the amino acid sequence of SEQ ID NO: 48, and mlcD has the amino acid sequence of SEQ ID NO: 50.