Patent ID: 8244484

Claim:
A method for achieving high sensitivity detection and/or high accuracy quantitation of a target protein in a biological sample, the method comprising the steps of: (1) providing an addressable array of two or more different capture agents wherein each of which two or more capture agents is specific for binding naturally occurring peptide epitope tag (PET) within the target protein; (2) contacting the addressable array of two or more different capture agents with a solution of polypeptide analytes produced by denaturation and/or cleavage of the target protein in the test sample. such that the capture agents have access to the peptide epitope tags within the polypeptide analytes generated by the denaturation and/or cleavage of the target protein; (3) detecting a signal generated as a result of binding of a capture agent to a naturally occurring PET within a polypeptide analyte; and (4) averaging the signals generated in (3) from each of the capture agents specifically binding to a naturally occurring PET, if signals are present, thereby to quantitate the amount of the target protein in the sample.