Patent ID: 8501495

Claim:
A method of performing a sequential solid phase immunoassay comprising the steps of: a) providing a sequential solid phase immunoassay system including a test strip having a first absorbent material disposed at a first end thereof and a second absorbent material disposed at a second end thereof, a fluid specimen application zone disposed between the first and second absorbent materials and proximate to the first absorbent material, an antigen immobilized on the test strip at a capture zone, the capture zone disposed between the first and second absorbent materials and distally of the first absorbent material; b) applying a stabilized liquid secondary antibody conjugate to the first absorbent material, wherein said stabilized liquid secondary antibody conjugate flows towards the capture zone and through the fluid specimen application zone; c) applying a fluid specimen to the fluid specimen application zone immediately after said application of the secondary antibody conjugate to the first absorbent material, wherein said fluid specimen application zone is in the path of said flow of said stabilized liquid secondary antibody conjugate, wherein said fluid specimen is applied onto said flow of said stabilized liquid secondary antibody conjugate, and wherein said flow of said stabilized liquid secondary antibody conjugate carries the applied fluid specimen to the capture zone; and d) reading the results at the capture zone.