Patent ID: 7601534

Claim:
A method for maintaining a non-differentiated state of human stem cells, while allowing cell division of said human stem cells, comprising repeatedly administering to human stem cells in a cell concentration of about 1 to about 10 10 cells per ml an effective amount of an inhibitor of cell proliferation of cell development in sequential combination with an anti-inhibitor in a controlled manner to maintain the non-differentiated state of stem cells, while allowing their cell division until the amplification of the cells is sufficient to obtain a pre-determined number of cells, wherein said anti-inhibitor is anti-TGFβ in an amount of 0.1 μg to 10 mg/ml, and wherein said inhibitor is TGFβ in an amount of 0.01 pg/ml to 1 mg/ml and said human stem cells are hematopoietic stem cells.