Patent ID: 7611855

Claim:
An immunoassay method in which tartrate resistant acid phosphatase 5b (TRACP 5b) is assayed in a specimen containing the tartrate resistant acid phosphatase 5b (TRACP 5b) together with a competitive substance therein, the method comprising: providing a first antibody that is Trk62 produced by a hybridoma deposited under the Budapest Treaty at National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary, under the accession number of FERM BP-7890, and a second antibody that is Trk49 produced by a hybridoma deposited under the Budapest Treaty at National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary, under the accession number of FERM BP-8249, bonding the tartrate resistant acid phosphatase 5b and the competitive substance in the specimen to the first antibody and second antibody, wherein the first antibody and second antibody are both adsorbed on a first carrier, or wherein the first antibody is adsorbed on a first carrier and the second antibody is adsorbed on a second carrier dispersed in a solution or is dissolved in a solution, and then measuring the level of the bonded tartrate resistant acid phosphatase 5b to assay the amount of tartrate resistant acid phosphatase 5b in said specimen, wherein the measurement of the level of the bonded tartrate resistant acid phosphatase 5b is the measurement of the amount of enzymatic activity of the bonded tartrate resistant acid phosphatase 5b, and wherein the competitive substance is a substance not having enzymatic activity of tartrate resistant acid phosphatase 5b.