Patent ID: 8354272

Claim:
An expression cassette comprising: a) a bacterial promoter, p Zn , comprising a binding site for the Lactococcus lactis ZitR protein, which site comprises the following sequence: AAAAATAANGTNNNNNNNTTGACATTATTTTT, (SEQ ID NO: 1) in which TTGACA is the −35 box of said promoter, and N represents A, C, G or T; and b) a sequence encoding a Lactococcus ZitR polypeptide, said polypeptide having at least 85% identity with the Lactococcus lactis ZitR protein encoded by nucleotides 357-794 of SEQ ID NO: 9 c) a sequence containing at least one restriction site for inserting a nucleotide sequence of interest; wherein said sequences b) and c) are under transcriptional control of the promoter, p Zn , and wherein said expression cassette does not comprise any part of the ZitS coding sequence.