Patent ID: 8227193

Claim:
A method for identifying one or more test compounds that alters binding of RelB Rel homology domain (RelB RHD) with RelBκB sequence, comprising: a) providing i) an isolated nucleotide sequence comprising 5′-NGGAGANNTG-3′ (SEQ ID NO:57); wherein N at position 1 is chosen from G and A, N at position 7 is chosen from T and C, and N at position 8 is chosen from T and C, and wherein said isolated sequence specifically binds with a polypeptide sequence comprising RelB Rel homology domain (RHD) as comprising SEQ ID NO:62, ii) a polypeptide comprising RelB RHD comprising SEQ ID NO:62, and iii) one or more test compounds; b) contacting said isolated nucleotide sequence with said polypeptide in the presence and absence of said one or more test compounds; and determining the level of specific binding of said nucleotide sequence with said polypeptide in the presence of said one or more test compounds compared to in the absence of said one or more test compounds, wherein detecting altered specific binding of said nucleotide sequence with said polypeptide in the presence of said one or more test compounds compared to in the absence of said one or more test compounds identifies said one or more test compounds as altering binding of RelB RHD with RelBκB sequence.