Patent ID: 7652194

Claim:
A process for producing transgenic plant comprising and expressing a transgenic coding sequence of interest under transcriptional control of an endogenous plant nuclear promoter, comprising (a) introducing into the nuclear genome of a plant cell a vector comprising a nucleotide sequence that encodes a nucleic acid sequence that binds a plant cytoplasmic ribosome, said vector further comprising a translational stop signal upstream of said nucleic acid sequence, wherein said nucleic acid sequence that binds a plant cytoplasmic ribosome is a plant viral internal ribosome entry site (IRES) of a crucifer-infecting tobamovirus, said vector further comprising a transgenic coding sequence of interest downstream of said nucleotide sequence, wherein the transgenic coding sequence of interest is not operationally linked to a promoter in the vector and is transcribed by the endogenous plant promoter and translated by the IRES of the vector; and (b) selecting a plant expressing said transgenic coding sequence of interest.