Patent ID: 7935510

Claim:
A recombinant gene expression modulation system comprising a recombinant gene expression cassette that is capable of being expressed in a host cell, the gene expression cassette comprising a polynucleotide that encodes a first polypeptide comprising: (a) a transactivation domain; (b) a DNA-binding domain that recognizes a response element associated with a gene whose expression is to be modulated; and (c) a nuclear receptor ligand binding domain that is capable of binding a diacylhydrazine and comprises either an amino acid residue substitution mutation of SEQ ID NO: 1 at (i) at least one of amino residues 54, 119, 127, 128 and 132, (ii) amino acid residues 96 and 119, (iii) amino acid residues 110 and 128, (iv) amino acid residues 52 and 110, (v) amino acid residues 132 and 125 (vi) amino acid residues 107, 110 and 127, (vii) amino acid residues 52, 107 and 127, or (viii) amino acid residues 107, 127 and an insertion at 259, or at least one of the mutations F48Y, F48W, F48L, F48N, F48R, F48K, I51M, I51N, 151L, T52M, T52E, T52P, T52R, T52W, T52G, T52Q, M92L, M92E, R95H, R95M, R95W, V96L, V96W, V96S, V96E, V96T, V1071, F109W, F109P, F109L, F109M, F109N, A110E, A110N, A110P, A110W, Y120W, Y120M, M125P, M125R, M125E, M125L, M125C, M125W, M125G, M125I, M125N, M125S, M125V, Y127E, M219K, M219W, M219Y, M219A, L223K, L223R, L223Y, L234M, L2341, L234R, L234W, W238P, W238E, W238Y, W238M and W238L.