Patent ID: 8637653

Claim:
An Alternanthera mosaic virus (AItMV) bipartite launch system comprising: (a) a first construct comprising an AItMV RNA-dependent RNA polymerase (RdRp), operably linked to a CaMV 35S promoter or other suitable plant promoter and a T7 promoter or other suitable bacteriophage promoter; (b) a second construct comprising in order an AItMV partial RdRp, shown schematically in FIG. 19D , an AItMV Triple Gene Block 1 (TGB1), an AItMV Triple Gene Block 2 (TGB2), an AItMV Triple Gene Block 3 (TGB3), a subgenomic promoter operably linked to a multiple cloning site (MCS), and a subgenomic promoter operably linked to an AItMV Coat Protein (CP); and (c) a third construct encoding a T7 RNA polymerase (T7RNAP); wherein the second construct is operably linked to a CaMV 35S promoter or other suitable plant promoter and a T7 promoter or other suitable bacteriophage promoter, the TGB1 amino acid sequence is the sequence set forth in SEQ ID NO: 84 or SEQ ID NO: 88, the first and second construct undergo recombination in planta within a short overlap of a common sequence of the partial RdRp present in both constructs, and the AItMV bipartite launch system is capable of high level protein expression in a plant.