Patent ID: 8828684

Claim:
An isolated eukaryotic host cell comprising one or more heterologous nucleic acids that are integrated into the host cell's chromosome wherein the chromosomally integrated heterologous nucleic acids encode: a) an enzyme that converts 3-hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) to mevalonate; b) farnesyl diphosphate (FPP) synthase; and c) a repressible promoter that controls transcription of the endogenous gene encoding squalene synthase; wherein the host cell further comprises a heterologous terpene synthase, wherein the terpene synthase catalyzes conversion of FPP, produced by action of the FPP synthase, into an isoprenoid, and produces the isoprenoid under culture conditions that provide for repression of transcription of the endogenous squalene synthase-encoding gene, and wherein FPP does not accumulate at levels that are toxic to the cell.