Patent ID: 8203032

Claim:
A method for using a Chiridius poppei -derived fluorescent protein for generation of a transformed plant capable of emitting fluorescence, characterized in that said Chiridius poppei -derived fluorescent protein used therefor is a Chiridius poppei -derived wild-type yellowish-green fluorescent protein (CpYGFP) comprising the following amino acid sequence (SEQ ID NO: 1): MTTFKIESRI HGNLNGEKFE LVGGGVGEEG RLEIEMKTKD KPLAFSPFLL SHCMGYGFYH 60 FASFPKGTKN IYLHAATNGG YTNTRKEIYE DGGILEVNFR YTYEFNKIIG DVECIGHGFP 120 SQSPIFKDTI VKSCPTVDLM LPMSGNIIAS SYARAFQLKD GSFYTAEVKN NIDFKNPIHE 180 SFSKSGPMFT HRRVEETHTK ENLAMVEYQQ VFNSAPRDM 219 as its full-length amino acid sequence, a wild-type plant used in the process for generation of the aimed transformed plant capable of emitting fluorescence is a plant which is capable of undergoing Agrobacterium infection and producing a harvestable T1 seed through the self-pollination of each plant individual, and the process for generation of the aimed transformed plant capable of emitting fluorescence comprises steps of: inserting a DNA fragment encoding the full-length amino acid sequence of the Chiridius poppei -derived fluorescent protein into the cloning site of a T-DNA-based binary vector; introducing the obtained T-DNA-based binary vector into a host bacterium of the genus Agrobacterium such that the bacterium of the genus Agrobacterium is transformed therewith; infecting the wild-type plant with the obtained transformant of the bacterium of the genus Agrobacterium such that a T-DNA region contained in the T-DNA-based binary vector is recombinantly introduced in the chromosomal DNA of the wild-type plant to obtain a transformed plant; subjecting each individual of the obtained transformed plant to self-pollination, and then harvesting a T1 seed from the plant individual; and sowing the obtained T1 seed and screening each grown plant to select a transformed plant individual to which a fluorescent phenotype attributed to the recombinant expression of the fluorescent protein in the leaf surface of the plant has been imparted.