Patent ID: 8649581

Claim:
A method of determining a color transformation for images of biological material, comprising the acts of: preparing a first set of biological test objects using a first staining method, the set comprising at least one test object; preparing a second set of the biological test objects using a second staining method, each test object in the second set of test objects corresponding to a counterpart test object in the first set of test objects, the test object and its counterpart being of the same biological type of material, wherein the first staining method is different from the second staining method; for each test object in the first set of test objects stained by the first staining method, determining a color of the test object, thereby generating a first set of colors; for each test object in the second set of test objects stained by the first staining method, determining a color of the test object, thereby generating a second set of colors; generating a conversion table indicating a mapping between the colors in the first set of colors resulting from the first staining method and the corresponding colors in the second set of colors resulting from the second staining method; preparing a first sample containing the first set of biological test objects; and preparing a second sample containing the second set of biological test objects; and creating a hardened block containing biological material; wherein preparing the first sample comprises cutting from the hardened block a first slice, and preparing the second sample comprises cutting from the hardened block a second slice parallel to the first slice.