Patent ID: 8779238

Claim:
A method of transforming a Camelina plant comprising within a first dipping cycle, i) contacting the Camelina plant with a first transformation dipping solution comprising a sugar, a nonionic surfactant, and an Agrobacterium comprising a first expression vector capable of expression in the Camelina plant, wherein the contacting step comprises dipping the Camelina plant into the solution; ii) removing the plant from the first transformation dipping solution, thereby conducting a first contacting step to produce a first dipped plant; iii) incubating the first dipped plant; iv) in the same first dipping cycle, contacting the first dipped plant with a second transformation dipping solution comprising a sugar, a nonionic surfactant, and an Agrobacterium comprising a second expression vector capable of expression in the Camelina plant, thereby conducting a second contacting step to produce a twice dipped plant, wherein the contacting step comprises dipping the Camelina plant into the solution; v) removing the twice dipped plant from the second transformation dipping solution; and vi) incubating the twice dipped plant; wherein the sugar is a disaccharide, the nonionic surfactant is trisiloxane, and both flowers and buds are present during the dipping steps; wherein said method does not involve vacuum infiltration.