Patent ID: 8480952

Claim:
A method of manufacturing a liposome-containing preparation comprising mixing a supercritical carbon dioxide, one or more liposome membrane constituents including a phospholipid exhibiting a phase transition temperature and a water-soluble chemical, and the method comprises the steps of: (a) mixing one or more liposome membrane constituents and liquefied carbon dioxide in a pressure vessel, (b) increasing a pressure within the vessel with heating at a temperature of 32 to 55° C. to bring the carbon dioxide to a supercritical state, (c) supplying an aqueous solution of the water-soluble chemical to a mixture of the liposome membrane constituent and the supercritical carbon dioxide with stirring to form a mixed solution, and (d) decreasing the pressure within the vessel to discharge the carbon dioxide from the mixed solution to form an aqueous dispersion of liposomes enclosing the water-soluble chemical wherein the liposome membrane constituents include a compound containing a polyethylene glycol group, and in step (c), stirring is subjected to strong agitation satisfying the following requirement: C=N×V −0.15 wherein C is a number of revolutions per minute (rpm), V is a volume (L) of a mixed solution and N is 300 to 3000.