Patent ID: 7335495

Claim:
A process for the isolation of the pharmaceutically active compound cyclosporin A from the fungus Fusarium nivale , comprising the steps of: a) washing the leaves of Porteresia coarctata with sterile sea water, b) allowing the fungus to grow and sporulate onto the leaves in moisture condition, c) identifying the fungus grown onto the leaves as Fusarium nivale , d) purifying a colony of fungus Fusarium nivale of step (c), e) transferring the purifed colony of step (d) by a nichrome loop into sterile culture media, f) growing the culture media of step (e) containing nutrient both with sea water and standard medium of three weeks under stationary condition, g) removing biomass of the fungi of step (d) after a predetermined growth is achieved, h) freeze drying the biomass of step (g), i) extracting the freeze dried biomass of step (h) with a ketonic solvent, j) concentrating the ketonic solvent of step (i) under reduced pressure and a temperature of about 30° C. to obtain an extract, k) adding water to the extract of step (j), l) extracting the aqueous extract of step (k) with petroleum ether, followed by ethyl acetate, separating the organic layers and aqueous solution, m) evaporating the ethyl acetate extract of step (l) to obtain concentrated ethyl acetate extract, and n) purifying concentrated ethyl acetate extract of step (in) by repeated gel chromatography using Sephadex LH-20, eluting with cholorform-methanol (1:1) to obtain pure cyclosporin A.