Patent ID: 7371545

Claim:
A method for determining the amount of template nucleic acid present in a sample comprising: i) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification including: a) a nucleic acid polymerase, b) the substrates for the nucleic acid polymerase, c) at least two primers, d) a thermostable luciferase, e) luciferin, f) ATP sulphurylase, and g) adenosine 5′ phosphosulphate; and subsequently: ii) performing a nucleic acid amplification reaction of the template nucleic acid involving more than one cycle of amplification; iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.