Patent ID: 7097995

Claim:
A chemiluminescent substrate of a hydrolytic enzyme, said substrate having the structure wherein P is PO 3 B wherein B is a divalent cation selected from cations of the group consisting of Ca and Mg, or two monovalent cations selected from the group consisting of Na, H, K M is oxygen; R 1 is an alkyl, alkenyl, alkynyl or aralkyl containing 0 to 20 heteroatoms; R 2a , R 2b , R 2c , R 3a , R 3b , R 3c and R 3d can be the same or different and are selected from the group consisting of hydrogen, methyl, methoxy, halide and cyano (—CN); R 12 , R 13 , R 14 and R 15 can be the same or different and are selected from the group consisting of hydrogen, —R, substituted or unsubstituted aryl, halides, nitro, sulfonate, sulfate, phosphonate, —CO 2 H, —C(O)OR, cyano (—CN), —SCN, —OR, —SR, —SSR, —C(O)R, —C(O)NHR, ethylene glycol and polyethylene glycol, where R is an alkyl group having 1 to 6 carbon atoms; A − is a counter ion for the electroneutrality of the quaternary nitrogen of the acridinium compound, said A − not being present if said R 1 substituent contains a strongly ionizable group that can form an anion and pair with the quaternary ammonium cationic moiety; and X is selected from the group consisting of O, N and S, such that, when X is O or S, Y is selected from the group consisting of phenyl, (2′-methyl)phenyl, (2′-methoxy)phenyl, (2′,6′-dimethyl)phenyl, (2′,6′-dimethoxy)phenyl, (2′-methyl-6′-methoxy)phenyl, (2′,6′-dimethyl-4′-benzyloxycarbonyl)phenyl, (2′,6′-dimethoxy-4′-benzyloxycarbonyl)phenyl, (4′-methyl)phenyl, (2′-methyl-6′-methoxy-4′-benzyloxycarbonyl)phenyl, (2′,6′-dimethyl-4′-carboxyl)phenyl, (2′,6′-dimethoxy-4′-carboxyl)phenyl and (2′-methyl-6′-methoxy-4′-carboxyl)phenyl, and Z is omitted, and when X is nitrogen, Z is —SO 2 —Y′, Y is as defined above, Y′ being defined the same as Y above, or Y and Y′ can each be a branched or straight-chain alkyl containing 0 to 20 carbons, halogenated or unhalogenated, a substituted aryl or heterocyclic ring system, and Y and Y′ can be the same or different.