Patent ID: 6884873

Claim:
A kit for determining DNA double-stranded breaks, wherein said kit comprises (i) an isolated or purified antibody or antigenically-reactive fragment thereof that binds to a C-terminal amino acid sequence of an H2A histone protein, said C-terminal amino acid sequence consisting of SQ(D/E/A)(I/L/Y/F) (SEQ ID NO:1) that comprises a phosphorylated serine, wherein the antibody or antigenically reactive fragment thereof does not detectably bind to a C-terminal amino arid sequence of an H2A histone protein, said C-terminal amino acid sequence consisting of SQ(D/E/A)(I/L/Y/F) (SEQ ID NO:1) that does not comprise a phosphorylated serine under conditions when the isolated or purified antibody or antigenically-reactive fragment thereof binds to the C-terminal amino acid sequence of an H2A histone protein, said C-terminal amino acid sequence consisting of SQ(D/E/A)(I/L/Y/F) (SEQ ID NO:1) that comprises a phosphorylated seine, and (ii) a means of facilitating detection of binding of said antibody or antigenically-reactive fragment thereof to an H2A histone protein.