Patent ID: 8334115

Claim:
A method for producing a saccharide chain of Formula (3), the method comprising at least the step of causing a reagent to contact with a GalNAc donor and a saccharide chain of Formula (1): GlcUA-GalNAc-R1 (1), GalNAc-GlcUA-GalNAc-R1 (3), where GlcUA is an N-glucuronic acid residue, GalNAc is an N-acetylgalactosamine residue, “-” indicates a glycoside linkage, and R 1 is an arbitrary group, and the reagent has an enzyme that has an amino acid sequence including an amino acid sequence (A) or (B) and has catalytic activities (α) and (β); wherein: (A) is the amino acid sequence from amino acid numbers 47 to 802 in SEQ ID NO: 2; (B) is the amino acid sequence from amino acid numbers 47 to 802 in SEQ ID NO: 2, wherein 1 to 40 amino acids in the amino acid sequence are substituted, deleted, or inserted; (α) is the catalytic activity that transfers GalNAc from UDP-GalNAc to chondroitin, where UDP is uridine 5′ diphosphate, and GalNAc is N-acetylgalactosamine residue; and (β) is the catalytic activity that transfers GlcUA from UDP-GlcUA to chondroitin, where UDP is uridine 5′ diphosphate and GlcUA is glucuronic acid residue.