Patent ID: 8551968

Claim:
A method for producing an antibody to an antigen comprising: a) providing a non-human mammal that inducibly overexpresses MYC, wherein the non-human mammal was produced by transferring a mammalian hematopoietic stem cell into the non-human mammal, wherein the mammalian hematopoietic stem cell comprises a recombinant nucleic acid molecule encoding an inducible MYC and a recombinant nucleic acid molecule encoding an antigen, and wherein the non-human mammal was lethally-irradiated or sub lethally-irradiated prior to having the hematopoietic stem cell transferred into the non-human mammal; b) inducing the overexpression of MYC in the non-human-mammal after formation of mature B cells from the transferred mammalian hematopoietic stem cell; c) recovering a B cell overexpressing MYC from the non-human mammal, wherein the B cell comprises the recombinant nucleic acid molecule encoding the inducible MYC and the recombinant nucleic acid molecule encoding the antigen; d) culturing the B cell overexpressing MYC under conditions in which MYC overexpression is maintained; and e) recovering the antibody from the B cell culture.