Patent ID: 8889371

Claim:
A method of preparing a lysoglycolipid comprising treating a substrate comprising a glycolipid with at least one lipolytic enzyme to produce said lysoglycolipid, wherein said lipolytic enzyme has glycolipase activity and wherein said lipolytic enzyme is obtainable from the genus Corynebacterium and wherein said lipolytic enzyme comprises at least one GDSx motif, wherein G is glycine, D is aspartic acid, S is serine and x is a hydrophobic amino acid residue selected from the group consisting of F, W, Y, H, K, M, I, L, V, A and G; or a GANDY block, wherein the GANDY block comprises the amino acid motif GGNDA or GGNDL, wherein G is glycine, N is asparagine, D is aspartic acid, A is alanine and L is leucine; or a HPT block, wherein H is histidine, P is proline and T is threonine wherein said lipolytic enzyme comprises an amino acid sequence shown as SEQ ID NO: 8 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID NO: 9 or a nucleotide sequence which has at least 70% identity therewith and which encode the said lipolytic enzyme.