Patent ID: 8173862

Claim:
A method for transferring one or more nuclear genes from a male fertile Eruca sativa plant to a cytoplasmic male sterile Eruca sativa plant, comprising the steps of: a) crossing a cytoplasmic male sterile Eruca sativa plant with a male fertile Eruca sativa plant, wherein said male sterile Eruca sativa plant comprises Ogura male sterile cytoplasm obtained from Brassica oleracea, b) harvesting one or more seed produced by the cross of step a), c) back-crossing a plant grown from one or more seed of step b) with said male fertile Eruca sativa plant, and harvesting seed produced by the back-cross, and d) back-crossing a plant grown from one or more harvested seed of step c), or seed from a subsequent back-crossing step, with said male fertile Eruca sativa plant for one or more back-cross generation, until one or more nuclear genes from the male fertile E. sativa are transferred to one or more male sterile backcross plant progeny.