Patent ID: 8900838

Claim:
A method for the fermentative production of 1,3-propanediol from sucrose, comprising the steps of: culturing a microorganism from the Enterobacteriaceae family genetically modified for the bioproduction of 1,3-propanediol from sucrose on an appropriate culture medium comprising sucrose, wherein the microorganism comprises: (i) an enzyme having a 2-keto acid decarboxylase activity for decarboxylation of 4-hydroxy-2-ketobutyrate encoded by the kivD gene of Lactococcus lactis , and (ii) an enzyme having hydroxy aldehyde reductase activity encoded by the gene yqhD of Escherichia coli for reduction of 3-hydroxypropionaldehyde obtained from decarboxylation of 4-hydroxy-2-ketobutyrate by the 2-keto acid decarboxylase, and (iii) functional genes coding for a non-PTS sucrose utilization system, enabling the microorganism to utilize sucrose as sole carbon source involving the genes cscBKA from E. coli , and wherein the production of 4-hydroxy-2-ketobutyrate from sucrose is improved in said microorganism by overexpressing a gene encoding homoserine transaminase or homoserine oxidase, and recovering 1,3-propanediol from the culture medium.