Patent ID: 8354274

Claim:
A cell culture article, comprising: a substrate having a surface; a swellable (meth)acrylate layer disposed on the surface of the substrate, wherein the swellable (meth)acrylate layer is formed from a composition comprising a carboxyl group-containing (meth)acrylate monomer, a cross-linking (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl gtow-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer, and a peptide conjugated to a carboxyl group of the carboxyl group-containing monomer of the swellable (meth)acrylate layer, wherein the peptide comprises an amino acid sequence selected from the group consisting of: LysGlyGlyAsnGlyGluProArgGlyAspThrTyrArgAlaTyr (SEQ ID NO:4); AsnGlyGluProArgGlyAspThrTyaArgAlaTyr (SEQ ID NO:5) LysGlyGlyLys 4 AsnGlyGluProArgGlyAspThrTyrArgAlaTyrAsp 17 (SEQ ID NO:6), where Lys 4 and Asp 17 together form an amide bond to cyclize a portion of the polypeptide; LysGlyGlyLys 4 GluProArgGlyAspThrTryArgAsp 13 (SEQ ID NO:7), where Lys 4 and Asp 13 together form an amide bond to cyclize a portion of the polypeptide; LysGlyGlyCys 4 AsnGlyGluProArgGlyAspThrTyrArgAlaTyrCys 17 (SEQ ID NO:8), where Cys 4 and Cys 17 together form a disulfide bond to cyclize a portion of the polypeptide; LysGlyGlyCys 4 GluProArgGlyAspThrTryArgCys 13 (SEQ ID NO:9), where Cys 4 and Cys 13 together form a disulfide bond to cyclize a portion of the polypeptide; GlyArgGlyAspSerProLys (SEQ ID NO:11); and LysGlyGlyAlaValThrGlyArgGlyAspSerProAlaSerSer (SEQ ID NO:12).