Patent ID: 8420363

Claim:
A method of making a device for detecting cells on an electrode surface through measurement of impedance change resulting from attachment of cells on the electrode surface, comprising: a) providing a non-conductive substrate with a deposited electrically conductive metal film; b) patterning said electrically conductive metal film using laser ablation of said conductive film from said substrate to form an array of electrodes, wherein each electrode comprises at least two electrode structures having a thickness up to 230 nm and that permit impedance measurement at each electrode structure, wherein each electrode structure comprises a plurality of electrode elements; and c) cleaning said substrate with an acid or base solution to remove re-deposition of ablated conductive material; d) connecting said electrode structures to a surface of said substrate opposite said electrode structures with a means for electronic connection selected from the group consisting of a flex circuit, metal clips, electrically conductive lines formed through said substrate, electrical wires and electrically conductive paste filled holes; and e) assembling said substrate to a bottomless multi-well plate to form an electrode-containing multi-well plate; wherein said electrode structures are positioned within wells of said multi-well plate.