Patent ID: 7354726

Claim:
A method of screening a compound or salts thereof that alters the binding property between a G protein-coupled receptor protein or salts thereof, and a bile acid, wherein said method comprises: a) contacting a labeled bile acid with the G protein-coupled receptor protein containing the amino acid sequence represented by SEQ ID NO: 1 or a protein containing an amino acid sequence having at least 90% homology to the amino acid sequence represented by SEQ NO: 1 and having the same ligand binding activity as the amino acid sequence represented by SEQ ID NO: 1 or salts thereof, and measuring the amount of said labeled bile acid bound to said G protein-coupled receptor protein or said protein or salts thereof; and b) contacting a labeled bile acid and a test compound with the G protein-coupled receptor protein containing the amino acid sequence represented by SEQ ID NO: 1 or a protein containing an amino acid sequence having at least 90% homology to the amino acid sequence represented by SEQ NO: 1 and having the same ligand binding activity as the amino acid sequence represented by SEQ ID NO: 1 or salts thereof, and measuring the amount of said labeled bile acid, bound to said G protein-coupled or said protein or salts thereof; and c) comparing binding property between a) and b).