Patent ID: 8759092

Claim:
A method of making a composition comprising inactivated tumor cells, wherein the composition comprising the inactivated tumor cells is administered to a patient, the method comprising: removing a tumor from the patient; purifying at least some tumor cells from the tumor; containing the tumor cells in a photopermeable container for radiation; adding an endogenous photosensitizer to the photopermeable container, wherein the endogenous photosensitizer comprises 7,8-dimethyl-10-ribityl isoalloxazine, and wherein the endogenous photosensitizer is at a concentration of at least about 10 μM; inactivating the tumor cells by exposing the tumor cells to the endogenous photosensitizer and light at a sufficient wavelength to prevent replication of the tumor cells but not substantially destroy the antigenic determinants of the tumor cells, wherein the exposing comprises: irradiating the tumor cells and the endogenous photosensitizer in the photopermeable container at a range of between about 1 to about 120 J/cm 2 for a period of between about 6 to about 36 minutes; suspending the inactivated tumor cells in a suspension solution to make the composition comprising the inactivated tumor cells, wherein the suspension solution comprises one or more from the group consisting of: saline and water; and administering the composition comprising the inactivated tumor cells to the patient subcutaneously, intramuscularly, intraperitoneally, orally, or nasally.