Patent ID: 7825233

Claim:
A nucleic acid molecule comprising a modified open reading frame encoding an active BoNT/E wherein the modification comprises nucleotide changes that alter at least 500 synonymous codons to those preferred by an Escherichia coli cell as compared to an unmodified open reading frame encoding the same active BoNT/E; wherein the active BoNT/E executes the overall cellular mechanism whereby the active BoNT/E enters a neuron and inhibit neurotransmitter release and encompasses the binding of the active BoNT/E to a low or high affinity receptor complex, the internalization of the active BoNT/E/receptor complex, the translocation of the active BoNT/E light chain into the cytoplasm and the enzymatic modification of the active BoNT/E substrate; and wherein the modified open reading frame comprises SEQ ID NO: 4.