Patent ID: 8380444

Claim:
A method of measuring a concentration of a component in a biochemical sample, the method comprising: injecting a plurality of standard samples into a plurality of chambers of a first centrifugal microfluidic device, wherein the plurality of standard samples includes at least one common component, and each of the plurality of standard samples has a different concentration of the component; measuring absorbances of the component in each of the plurality of standard samples contained in the chambers of the centrifugal microfluidic device at a plurality of different wavelength bands by emitting from a plurality of light sources a plurality of light beams having the plurality of wavelength bands to the plurality of standard samples and sensing by a plurality of optical detectors intensities of the plurality of light beams that have passed through the plurality of standard samples; determining a regression equation of a relationship between the concentration of the component and the measured absorbances of the component in the plurality of standard samples at the plurality of wavelength bands; and measuring absorbances of the biochemical sample contained in a plurality of chambers of a second centrifugal microfluidic device at the plurality of wavelength bands by emitting from the plurality of light sources the plurality of light beams having the plurality of wavelength bands to the biochemical sample and sensing by the plurality of optical detectors intensities of the plurality of light beams that have passed through the biochemical sample and applying the measured absorbances of the biochemical sample to the regression equation to calculate the concentration of the component in the biochemical sample.