Patent ID: 7531646

Claim:
A chromogenic enzyme substrate of the formula I in which R 1 is an enzyme cleavable group selected from the group consisting of β-D-galactopyranosyl, α-D-galactopyranosyl, decanoyl, octanoyl, inositol-1-phosphatyl, phosphatyl, β-D-ribofuranosyl, α-L-arabinopyranosyl, butyryl, nonanoyl, palmitoyl, lignoceroyl, α-L-fucopyranosyl, β-L-fucopyranosyl, α-D-fucopyranosyl, β-D-fucopyranosyl, α-D-mannopyranosyl, β-D-mannopyranosyl, oleoyl, sulphatyl, α-D-xylopyranosyl and β-D-xylopyranosyl; R 2 is hydrogen; Y has the structure of VII or VIII wherein each of R 5 , R 6 , R 7 and R 8 is selected from the group consisting of hydroxyl, C 1-24 alkyl, C 2-12 alkenyl, C 1-4 alkoxy, acyl, halogen, nitro, aryl and acyloxy groups, in which alkyl and aryl groups which are substituents may be substituted by amino, hydroxyl, peptide, acyloxy, alkoxy and aryl groups or two group R 5 and R 7 on the same carbon are=O; n is 0, 1 or 2; R 3 and R 4 are selected from the group consisting of H, C 1 to C 6 -alkyl, -alkoxy and hydroxyalkyl, halogeno, nitro, acyl, acyloxy, aralkyl, aryl and amido groups, wherein R 3 and R 4 are not linked to form a fused ring; or a salt or hydrate of such a compound.