Patent ID: 8552183

Claim:
A process for linking two molecules by means of a Diels Alder reaction with inverse electron requirement, comprising the step of: reacting (a) a triazine or tetrazine comprising one or more —C(O)NR 2 groups as electron-attracting substituents on the ring as a diene component, wherein R═H, alkyl, aryl, heterocycle, which is optionally substituted with alkyl, OH, SH, halogen, aryl, heterocycle, nitro, carboxyamido or amine group, with (b) an isolated double bond or triple bond in a heterocarbocyclic ring or an isolated olefinic double bond or triple bond in a linear or branched hydrocarbon chain which optionally contains heteroatoms as a dienophile component, wherein one or more peptides, saccharides, lipids, oligonucleotides, or nucleic acids are coupled to the diene and/or dienophile component.