Patent ID: 8202700

Claim:
A method to immobilize a molecule of interest, comprising the steps of: (a) contacting a sample comprising: (i) a fusion protein, the fusion protein comprising: (A) a mutant dehalogenase wherein said mutant dehalogenase comprises one or more amino acid substitutions relative to the corresponding wild-type dehalogenase, and wherein said one or more amino acid substitutions result in the mutant dehalogenase forming a stable covalent bond with a dehalogenase substrate; and (B) a second protein, wherein said second protein is capable of binding said molecule of interest; and (ii) said molecule of interest; with (iii) a solid support comprising the dehalogenase substrate; and (b) immobilizing said molecule of interest on said solid support as a result of said stable covalent bond formed between said mutant dehalogenase and said dehalogenase substrate.