Patent ID: 8168600

Claim:
A method for delivering an oligonucleotide preferentially to a hair follicle comprising: identifying a subject having skin comprising hair follicles; selecting a portion of skin comprising hair follicles on said subject for delivery of said oligonucleotide preferentially to hair follicles; and delivering a composition comprising an oligonucleotide in an aqueous solution to said portion of skin, wherein the aqueous solution is essentially free of any lipophilic compound or penetration enhancer, wherein said portion of skin is not pretreated with a penetration enhancer, and wherein skin adjacent to said hair follicles which is not part of said hair follicles provides a permeability barrier to said oligonucleotide such that said oligonucleotide preferentially accumulates in a dermal portion of said hair follicles as compared to a dermal layer of said skin adjacent to said hair follicles which is not part of said hair follicles.