Patent ID: 8129143

Claim:
A method for production of a polypeptide of interest in a eukaryotic cell containing a peroxisome that expresses a chimeric protein comprising a domain of a v-SNARE polypeptide that is normally exposed at the cytosolic side of a donor membrane of the secretory pathway, a transmembrane domain of a peroxisomal membrane protein and a sequence that targets the transmembrane domain to the peroxisomal membrane wherein said peroxisome fuses via the v-SNARE domain on the cytosolic side of its peroxisomal membrane with a membrane-structure of the cell involved in the secretory pathway of the cell wherein the membrane-structure of the cell involved in the secretory pathway of the cell is selected from the group consisting of the plasma membrane, the Golgi complex and the Endoplasmic Reticulum, wherein said polypeptide of interest is present in the peroxisome of the cell, said method comprising culturing the eukaryotic cell in a suitable culture medium.