Patent ID: 7374906

Claim:
A method of monitoring chemotaxis or chemoinvasion comprising: providing a support member having an upper surface; removably sealing a top member to the upper surface of the support member with substantially fluid tight, conformal contact to create a discrete assay chamber defining a plurality of chambers therein, each of the plurality of chambers including: a first well region including at least one first well; a second well region including at least one second well; and a channel region including at least one channel connecting the first well region and the second well region with one another, wherein the at least one channel is formed as a through-hole in the top member; introducing at least one soluble test substance in the at least one first well or the at least one channel; forming a dynamic solution concentration gradient along a longitudinal axis of the plurality of chambers; introducing cells in the at least one second well or the at least one channel; and monitoring chemotaxis or chemoinvasion of the cells.