Patent ID: 8735086

Claim:
A method for measuring the thrombin generation in whole blood or plasma, comprising the steps of: (a) providing a lyophilized tissue factor (TF)/phospholipid (PL)-complex and a lyophilized mixture that is dissolvable in water and contains a thrombin-substrate comprising a fluorescent label and CaCl 2 ; (b) mixing said lyophilized TF/PL-complex and said lyophilized mixture containing thrombin-substrate and CaCl 2 in an amount of plasma or whole blood sample that provides a concentration of 1 mM thrombin substrate and 15 mM CaCl 2 , wherein the lyophilized TF/PL-complex and the lyophilized mixture containing the thrombin-substrate and CaCl 2 are immobilized on a solid support; and (c) measuring the thrombin generation in said sample.