Patent ID: 7439017

Claim:
A method of assaying a sample for an enzyme activity that joins a first substrate with a second substrate to form a product or for a factor that affects the activity of said enzyme, wherein the presence, concentration, or activity of said enzyme or said factor is not known, comprising: (a) forming a composition comprising said sample, said first substrate and said second substrate, said first substrate being linked to a luminescent label and said second substrate being linked to a capture moiety; (b) incubating said composition under conditions wherein said enzyme can form said product at a differing rate in the presence or absence of said enzyme or factor, wherein said product is linked to said luminescent label and said capture moiety and wherein said enzyme or factor is not a part of the product; (c) capturing said capture moiety on an electrode; (d) applying voltage at said electrode so as to induce said luminescent label in said product to emit luminescence; and (e) measuring emitted luminescence so as to measure the presence of said enzyme or factor in said sample, wherein said enzyme is selected from the group consisting of nucleic acid polymerases, nucleic acid ligases, integrases, ribosomes, and trans-glutaminases.