Patent ID: 8227449

Claim:
A glycosaminoglycan derived from K5 polysaccharide, epimerized at least till 40% of iduronic acid with respect to the total uronic acids, having molecular weight from 2,000 to 30,000 D, containing from 25 to 50% on weight of the chains with high affinity for ATIII and having an anticoagulant and antithrombotic activity expressed as ratio HCII/Anti-Xa comprised between 1.5 and 4, as produced by a process comprising in sequence: (a) preparation of K5 polysaccharide from Escherichia coli , (b) N-deacetylation and N-sulfation of the K5 polysaccharide, (c) 40%-60% C5 epimerization of D-glucuronic acid to L-iduronic acid, (d) oversulfation, (e) selective O-desulfation, (f) selective 6-O sulfation, and (g) N-sulfation.