Patent ID: 8906947

Claim:
A method for separation and purification of epothilones B and A, characterized in that, the separation and purification of epothilones B and A are performed by a normal phase silica gel column chromatography; and the method comprises: dissolving a sample containing epothilones B and A in C 1 -C 7 alkyl halide compound(s) to form a mixture, wherein the mixture is mixed with silica gel or not; loading the mixture on a silica gel column; gradient eluting the silica gel column with a normal phase silica gel column eluent; collecting fractions; and obtaining products; wherein the normal phase silica gel column eluent is chosen from a C 1 -C 7 hydrocarbon, a C 1 -C 7 alkyl halide compound, a C 1 -C 7 ketone, C 1 -C 7 ester, or any combination thereof, and wherein the C 1 -C 7 hydrocarbon is chosen from petroleum ether, n-hexane, cyclohexane, n-heptane, or any combination thereof, the C 1 -C 7 alkyl halide compound is chosen from dichloromethane, trichloromethane, bromoethane, or any combination thereof, the C 1 -C 7 ketone is chosen from acetone, butanone, or any combination thereof, and the C 1 -C 7 ester is chosen from ethyl acetate, isobutyl acetate, or any combination thereof; and wherein the normal phase silica gel column is balanced with C 1 -C 7 alkyl halide compound(s) before use.