Patent ID: 8507263

Claim:
A bioreactor, comprising: a vessel; a cylindrical scaffold defining an outer wall of an inner chamber and an inner wall of an outer chamber, wherein the scaffold is removably positioned within the vessel and configured to rotate relative to the vessel about a central axis extending through the inner chamber; a shaft positioned within the inner chamber, the shaft comprising an opening defining a first inlet for introducing a liquid into the inner chamber; a removable flange which is operatively connected to the shaft, wherein the scaffold is attached to the flange, and wherein the flange is configured to rotate with the scaffold about the central axis; one or more mixing elements that are connected to the flange such that they are positioned within the outer chamber and co-rotate with the scaffold to enhance fluid motion and mixing in the outer chamber; a first outlet in fluid communication with the inner chamber; and an opening in fluid communication with the outer chamber for introducing and/or removing a liquid from the outer chamber.