Patent ID: 7871793

Claim:
An in vitro method of producing a glycoprotein having at least one α1,4-linked N-acetylglucosamine (α1,4-linked GlcNAc) residue, comprising contacting, under conditions suitable for glycosylation of a polypeptide, milk of a mammal containing a carrier polypeptide with an α1,4-N-acetylglucosaminyl transferase (α4GnT), and optionally, a core2 β1,6-N-acetylglucosaminyl transferase-I (C2GnT-I) or a corel extension β1,3-N-acetylglucosaminyl transferase (C1-β3GnT); wherein the carrier polypeptide is a mucin-type glycoprotein that includes at least one O-glycosylation site, whereby the carrier polypeptide is glycosylated by the α4GnT, and optionally, by the C2GnT-I or by the C1-β3GnT, thereby producing a glycoprotein having at least one α1,4-linked GlcNAc residue.