Patent ID: 8828670

Claim:
A method for producing a dimeric monoclonal antibody or physiologically effective antibody fragment containing internal or external disulfide bonds, comprising the following steps: a) providing a cell-free lysate, obtained from eukaryotic cells selected from the group consisting of insect cells, HeLa cells, CHO cells, HEK cells, wheat germ cells, rabbit reticulocyte cells, yeast cells, protozoa cells and green alga, which contains functional microsomal vesicles, b) adding a nucleic acid coding the dimeric monoclonal antibody or antibody fragment to the lysate wherein said nucleic acid comprises a signal sequence capable of assisting the translocation of said antibody or antibody fragment formed with the nucleic acid into the microsomal vesicles, c) holding the lysate with the nucleic acid for a given time at a temperature in the range from 20° C. to 35° C., to allow the signal sequence to assist in the translocation of said antibody or said antibody fragment formed with the nucleic acid into the microsomal vesicles, and d) digesting the microsomal vesicles, whereby said antibodies or antibody fragments the proteins or peptides obtained thereby are optionally separated from the lysate.