Patent ID: 8288171

Claim:
A biochip comprising: a metal thin film on the surface of a substrate, configured to restrain autofluorescence of the substrate; a spacer disposed over the thin metal film and having a predetermined thickness; a plurality of capture molecules immobilized on the surface of the spacer; a first portion of the plurality of capture molecules that are specifically bound to target molecules; a second portion of the plurality of capture molecules that are non-specifically bound to fluorescent detection molecules; a first fluorophore layer comprising a plurality of fluorescent detection molecules coupled, by binding to the target molecule that are specifically bound, to the first portion of the plurality of capture molecules; and a second fluorophore layer comprising a plurality of fluorescent detection molecules non-specifically bound to the second portion of the plurality of capture molecules; wherein the predetermined thickness is of a thickness determined to cause constructive interference between light directly emitted from the first fluorophore layer and light emitted from the first fluorophore layer and reflected by the metal thin film, and to restrain light emitted from the second fluorophore layer.