Patent ID: 8206914

Claim:
An in vitro method of enriching a product of a nucleic acid-templated synthesis, the method comprising the steps of: (a) providing a first library of molecules comprising a plurality of reaction products of a nucleic acid templated synthesis, which are not nucleic acids, wherein each reaction product is covalently attached to a corresponding oligonucleotide that templated the synthesis of the reaction product, and wherein each oligonucleotide comprises a nucleotide sequence indicative of the reaction product associated therewith, and wherein a portion of said reaction products are capable of binding to a preselected binding moiety; (b) exposing said first library of molecules to said binding moiety under conditions to permit reaction product capable of binding said binding moiety to bind thereto, wherein the reaction product has a K d for the binding moiety of no less than 0.9 nM; (c) removing unbound reaction products; and (d) eluting bound reaction product from said binding moiety to produce a second library of molecules enriched at least 50-fold for reaction product that binds said binding moiety relative to said first library.