Patent ID: 8569035

Claim:
A sub-group I-S2 subtilase variant having protease activity comprising at least one or more of the following alterations wherein each position corresponds to a position of the amino acid sequence set forth in SEQ ID NO:3: of subtilisin BPN′ and wherein the variant is: V11I+I44V; V11I+L96LA; S9R+V11I+A15T+T22A; V11I+L96LA+A108C+A138C; V11I+N76D+A194P+A230V; V11I+V84I; V11I+V84I+K251R; V11I+V30I; V11I+V139L; V30I; V30I+V84I; V30I+V139L; S9R+A15T+T22A+V30I; V30I+V244R; V30I+K251R; V30I+V139L+Y167A+R170S+A194P+N218S; N76D+M175ML+A194P+A230V; and N76D+M175MI+A194P+A230V.