Patent ID: 8623597

Claim:
A bioassay apparatus comprising: a disk-shaped substrate having a groove radially extending on the disk-shaped substrate; a detecting element formed on the disk-shaped substrate, the detecting element comprising: positive electrodes formed on a first sidewall of the groove and negative electrodes formed on a second sidewall of the groove, the positive and negative electrodes being formed along a longitudinal direction of the groove and opposing each other; a detection surface formed on one of the positive and negative electrodes, the detection surface being treated to immobilize an end of a detecting substance on the detection surface; a reaction region sandwiched by the positive and negative electrodes for providing a field for interaction between the detecting substance immobilized on the detection surface and a target substance, wherein the reaction region contains a material which can undergo a reversible phase change between gel and sol at between room temperature and the optimal temperature for a reaction; and a power supply coupled with the positive and negative electrodes configured to form an alternating electric field in the reaction region before the interaction, so as to prepare the detecting substance to be reacted with the target substance; means for providing positional information and rotational synchronization information of the detecting elements; a dropping means configured to drip the target substance into the reaction region based on the positional information and rotational synchronization information; and a liquid pool provided in the center of the substrate configured to feed a cleaning liquid into the reaction region by rotating the substrate; wherein the power supply is further configured to apply a rectangular wave voltage in a positive direction or a negative direction to the reaction region for a first predetermined time period; the power supply is further configured to adjust the first predetermined time period according to a time required to relatively move the target substance to the detecting substance in the reaction region; the power supply is further configured to remove the rectangular wave voltage from the reaction region for a second predetermined time period; and the power supply is further configured to adjust the second predetermined time period according to a time required to perform the interaction between the detecting substance and the target substance.