Patent ID: 7927841

Claim:
A process for assaying a product sample in which material may be present that emanates from a particular type of animal selected from the group consisting of porcine species, and a ruminant as source, in order to determine, qualitatively or quantitatively, whether or the extent to which material from the particular type of animal is present in the product sample, said process comprising the following steps: (1) providing a product sample to be assayed; (2) extracting or isolating a DNA sample from the product sample; (3) carrying out a polymerase chain reaction (PCR) on said DNA sample with a primer pair to produce an amplified DNA product representative of a predetermined short interspersed element (SINE) selected from the group consisting of PRE-1 SINE of the porcine species, and a Bovine SINE family of the ruminant, said predetermined SINE when present in the DNA sample being a marker specific for the particular type of animal, said predetermined SINE selected from a genomic subset common to members of the particular type of animal and not found in genomes of other types of animal, the primer pair for the PRE-1 SINE of the porcine species comprising at least one of the primer pair (i) and the primer pair (ii), the primer pair (i) comprising: a forward primer containing the sequence: 5′ GACTAGGAACCATGAGGTTGCG 3′ (SEQ ID NO: 3); and a reverse primer containing the sequence: 5′ AGCCTACACCACAGCCACAG 3′ (SEQ ID NO: 4); the primer pair (ii) comprising: a forward primer containing the sequence: 5′ GGCCTTGCTCAGTGGGTTAA 3′ (SEQ ID NO: 10); and a reverse primer containing the sequence: 5′ GGGATCCAAGCCACATCTGT 3′ (SEQ ID NO:11) the primer pair for the Bovine SINE family of the ruminant comprising: a forward primer containing the sequence: 5′ CAGTCGTGTCCGACTCTTTGT 3′ (SEQ ID NO: 7); and a reverse primer containing the sequence: 5′ AATGGCAACACGCTTCAGTATT 3′ (SEQ ID NO: 8); and (4) determining whether, or the extent to which a predetermined amplicon representative of the predetermined SINE is present in the amplified DNA product.