Patent ID: 7368296

Claim:
A method of modifying the surface of a solid support to enhance the quantum yield of chemiluminescent emissions, the method comprising: reacting a functional group on a chemiluminescent enhancing compound with functional groups on the surface of the solid support to covalently attach the chemiluminescent enhancing compound to the surface of the solid support; and attaching a plurality of immobilized probes for a biopolymer target to the surface of the solid support, wherein the chemiluminescent enhancing compound has the general formula: wherein Q is N or P; LINK is a divalent linker moiety; R 1 , R 2 and R 3 are, independently, an alkyl group, an aryl group or a nitrogen heterocycle; R is hydrogen, an alkyl group, or an aryl group; and X − is a counterion; and wherein the step of covalently attaching the chemiluminescent enhancing compound to the surface of the solid support comprises reacting the amino group on the quaternary onium compound with the functional groups on the surface of the solid support.