Patent ID: 8685730

Claim:
A method of culturing human pluripotent stem cells to produce cells of the pancreatic lineage, the method comprising the steps of: (a) culturing the stem cells under conditions that induce formation of mesendoderm/primitive streak and definitive endoderm cells in a chemically defined medium comprising an effective amount of i) fibroblast growth factor, ii) Activin A, and iii) bone morphogenetic protein; (b) culturing the cells from step (a) in the presence of a chemically defined medium comprising an effective amount of insulin, transferrin, and selenium, wherein the medium further comprises a fibroblast growth factor in an amount that ranges from about 10 ng/ml to about 200 ng/ml; and (c) culturing the cells under conditions to produce foregut/pancreatic progenitor PDX1 + Ins âˆ’ cells.