Patent ID: 8558058

Claim:
A method of increasing expression in a plant seed of a first nucleic acid molecule encoding a cellulase enzyme, the cellulase enzyme comprising an exo-1,4-β-glucanase enzyme, the method comprising incorporating said first nucleic acid molecule into a nuclear chromosome of a plant cell, said first nucleic acid molecule further comprising a promoter that functions in seeds operably linked to the molecule encoding the enzyme, and said first nucleic acid molecule further comprising a nucleotide sequence encoding a signal peptide effective for directing said enzyme into the secretory pathway, and said first nucleic acid molecule optionally further comprising a nucleotide sequence encoding an endoplasmic reticulum (ER) retention signal; wherein said enzyme is targeted to the cell wall or retained in the endoplasmic reticulum, and wherein expression of said enzyme is at higher levels than levels of expression for said enzyme when targeted to the vacuoles of said plant, such that said enzyme is expressed at levels of at least 1% total soluble protein in said seed.