Patent ID: 7871610

Claim:
An isolated protein comprising a heavy chain (HC) immunoglobulin variable domain sequence and a light chain (LC) immunoglobulin variable domain sequence, wherein the protein binds to Tie1 ectodomain and (A) the HC immunoglobulin variable domain sequence comprises: i) a HC CDR1 that comprises the amino acid sequence (AGSIMRH)-Y-(GVMK)-M-(GSVMFH) (SEQ ID NO:118); ii) a HC CDR2 that comprises the amino acid sequence (GSV)-I-(SY)-P-S-G-G-(WNQ)-T-(GY) (SEQ ID NO: 160); iii) a HC CDR3 that comprises the amino acid sequence A-P-R-G-Y-S-Y-G-Y-Y-Y (amino acids 99-109 of SEQ ID NO:114); and B) the LC immunoglobulin variable domain sequence comprises: i) a LC CDR1 that comprises the amino acid sequence R-A-S-(REQ)-(GSTRN)-(IV)-(GSTIRN)-(STIRH)-X1-(SYWNH)-(LV)-(ASN) (SEQ ID NO:132), wherein X1 is serine or absent; ii) a LC CDR2 that comprises the amino acid sequence (AGTKDEH)-A-S-(STN)-(LR)-(AVEQ)-(ST) (SEQ ID NO: 136); and iii) a LC CDR3 that comprises the amino acid sequence Q-Q-(SYFR)-(GSYN)-S-(STYW)-(RP)-(LWRH)-(TIY) (SEQ ID NO: 161).