Patent ID: 8361707

Claim:
A method for detecting Mycobacterium tuberculosis in a subject, comprising: contacting a biological sample from the subject, wherein the biological sample comprises T cells, with one or more isolated Mycobacterium polypeptide, wherein one of the isolated Mycobacterium polypeptides comprises the amino acid sequence set forth as SEQ ID NO: 8[, SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 11 or SEQ ID NO: 12]; and determining if the T cells specifically recognize the Mycobacterium polypeptide, wherein the presence of T cells that specifically recognize the Mycobacterium polypeptide detects Mycobacterium tuberculosis in the subject.