Patent ID: 8680024

Claim:
A method for forming an array of receptors for M13 bacteriophages, comprising: patterning an array of structures on a first substrate to form a template on a surface of the first substrate; applying a receptor material to a face of a second substrate, wherein the second substrate comprises a featureless elastomer having a planar surface, and wherein the receptor material comprises an antibody; contacting the face of the second substrate with the template to remove a portion of the receptor material from the second substrate, thereby forming an array of receptor material on the second substrate; contacting a target substrate with the array of the receptor material to transfer the array of the receptor material to the target substrate; and contacting the target substrate with a solution having a pH of 7 or higher comprising the M13 bacteriophages having a concentration of about 107 to about 109 plaque forming units/milliliter (pfu/mL), and transferring a portion of the M13 bacteriophages to the array of the receptor material, wherein the target substrate has a negative surface charge density and wherein the array of the receptor material has an average lateral dimension of the structures patterned on the substrate is about 60 nanometers to about 250 nanometers and wherein the average pitch between structures is about 5 micrometers to about 700 nanometers such that a majority of sites in the array of the receptor material are occupied by a single M13 bacteriophage whereby the majority of sites is at a 1:1 ratio of the single M13 bacteriophage to a selected one of the array of the receptor material, and wherein binding of the single M13 bacteriophage to the selected one occurs predominantly at an edge of the receptor material.