Patent ID: 7521019

Claim:
A device for detecting the presence of an analyte in a sample, comprising: (a) a core comprising: (i) a binding substrate comprising a binding site within a porous material, wherein the binding site comprises a molecular imprint of the analyte (ii) at least one analogue that binds in the binding site and that has a label with a first emission wavelength; (iii) a quenching dye located within the porous material of the binding substrate; and (iv) a void volume that is at least partially defined by the binding substrate; (b) a reference with a different emission wavelength than the label; and (c) an analyte-permeable membrane that completely encapsulates components (a) and (b) and that is transparent to light of the wavelengths that excite the label and the reference, wherein the device is seamless and wherein the analogue is capable of flowing in and out of the porous material into the void volume, while the quenching dye is not capable of leaving the porous material.