Patent ID: 6884584

Claim:
A method for identifying one or more nucleotide polymorphisms in a polynucleotide sample, the method comprising: providing an array of support-bound oligonucleotide probes of different sequences; incubating a polynucleotide sample with said array under hybridization conditions; incubating a plurality of different minor groove binder-oligonucleotide conjugate probes with said polynucleotide sample with said array under hybridization conditions to form one or more minor groove binder-oligonucleotide conjugate probe-target nucleic acid hybrids, wherein said minor groove binder is a molecule having a molecular weight of approximately 150 to approximately 2,000 Daltons that binds in a non-intercalating manner into the minor groove of a double-stranded nucleic acid with an association constant of greater than approximately 10 3 M − ; and detecting the presence of said minor groove binder-oligonucleotide conjugate probe-target nucleic acid hybrids on said array.