Patent ID: 7978893

Claim:
A method for determining biosensor binding, the method comprising: scanning an area of the biosensor; then in a processor; selecting a signal-region and a reference-region within the scanned area; scanning at least one sub-region within the signal-region and at least one sub-region within the reference-region; calculating a standard deviation for all Δλ points scanned in each sub-region; selecting all sampled points within each of the scanned sub-regions having a minimum standard deviation as a minimum signal sub-region and as a minimum reference sub-region; and calculating M sig , M ref , and B med , where Δλ is the difference in wavelength for two different time points, according to Δλ=λ t2 −λ t1 where λ t2 and λ t1 represent the wavelengths measured for respective second and first time points, M sig is the median value of all Δλ points in the selected minimum signal sub-region, M ref is the median value of all Δλ points in the selected minimum reference sub-region, and B med is the median biosensor binding value according to equation (1): B med =M sig −M ref (1).