Patent ID: 8597905

Claim:
A method of detecting, on a cytological slide, cells that have the ability to replicate, said slide being prepared and stained by Papanicolaou staining, said method comprising a) exciting said cells at a wavelength below 520 nm; b) measuring fluorescence emitted by said cells at a wavelength above 520 nm; wherein cells exhibiting an accumulation of fluorescence localized at their membrane envelope are capable of replication, wherein said cells are from a biological sample that has been fixed on a cytological slide and wherein Papanicolaou staining comprises submitting said slide to the following protocol Running water: 2 minutes Hematoxylin solution: 4 minutes Running water: 1 minute Solution comprising a mixture of 100% alcohol and of 0.2% HCI: 30 seconds Running water: 10 minutes Aqueous ammonia solution at 0.2%: 2 minutes 70% alcohol: 30 seconds 96% alcohol: 30 seconds OG6 solution: 4 minutes 96% alcohol: 30 seconds 96% alcohol: 30 seconds EA50 solution: 4 minutes 96% alcohol: 30 seconds 100% alcohol: 30 seconds Xylene: 30 seconds Xylene: 30 seconds.