Patent ID: 8492121

Claim:
A method for selective amplification of a nucleic acid sample, the method comprising: fragmenting the nucleic acid sample using a first restriction enzyme and a second restriction enzyme and producing a digested nucleic acid sample comprising fragments; ligating a double stranded first adaptor and a double stranded second adaptor to the fragments and producing ligated fragments, wherein the 3′ end of a first strand of the first adaptor is blocked from ligating to the 5′ ends of the fragments such that only a second strand of the first adaptor can ligate to the fragments, and wherein the 5′ end of a first strand of the second adaptor is blocked from ligating to the 3′ ends of the fragments such that only a second strand of the second adaptor can ligate to the fragments, wherein a subset of the ligated fragments comprises ligated fragments having a single strand which is ligated to the first adaptor at its 3′ end and the second adaptor at its 5′ end; and subjecting the ligated fragments to amplification conditions, and selectively and exponentially amplifying the ligated fragments having a single strand which is ligated to the first adaptor at its 3′ end and the second adaptor at its 5′ end, thereby selectively amplifying the nucleic acid sample.