Patent ID: 6916635

Claim:
A method for producing a recombinant vector comprising: a) providing: i) a first recombinant vector, comprising in operable combination: 1) a nucleotide sequence of interest having a 5′ end and a 3′ end; 2) left and right inverted terminal repeats of adenovirus flanking said nucleotide sequence of interest; 3) adenovirus packaging sequence linked to one of said inverted terminal repeats; and 4) a first adeno-associated virus terminal repeat sequence operably linked to said 3′ end of said nucleotide sequence of interest, wherein said first vector lacks a second adeno-associated virus terminal repeat sequence, and lacks one or more adenovirsus early gene regions selected from E1, E2, E3, and E4 gene regions; ii) a cell capable of expressing one or more Rep proteins; and iii) helper adenovirus; b) introducing said first vector and genome of said helper adenovirus into said cell to produce a transformed cell; and c) culturing said transformed cell under conditions such that said transformed cell expresses said one or more Rep proteins, and a second vector is produced, said second vector selected from: i) a vector, comprising in operable combination: 1) adeno-associated virus terminal repeat-DD sequence; 2) first and second inverted copies of a nucicotide sequence of interest flanking said adeno-associated virus terminal repeat-DD sequence; 3) left and right inverted terminal repeats of adenovirus flanking said first and second inverted copies of said nucleotide sequence of interest; and 4) an adenovirus packaging sequence linked to one of said inverted terminal repeats, and ii) a vector, comprising in operable combination: 1) a nucleotide sequence of interest having a 5′ end and a 3′ end; 2) left and right inverted terminal repeats of adenovirus flanking said nucleotide sequence of interest; and 3) an adenovirus packaging sequence linked to one of said inverted terminal repeats.