Patent ID: 8278105

Claim:
A method of inducing human hepatocyte cells from human embryonic stem cells, comprising: a) providing a quantity of human embryonic stem (hES) cells; b) placing the hES cells on an extracellular matrix and in a medium containing one of: i) neurobasal medium, DMEM, N2, B27, L-glutamine, β-ME, SU5402, PD184352, and CHIR99021, ii) neurobasal medium, DMEM, N2, B27, L-glutamine, β-ME, SU5402, PD184352, CHIR99021 and dorsomorphin, or iii) neurobasal medium, DMEM, N2, B27, L-glutamine, β-ME, PD0325901, CHIR99021, and dorsomorphin; and c) culturing the hES cells to form hepatocyte cells.