Patent ID: 7812006

Claim:
A pneumococcus type 5 capsular polysaccharide, wherein the polysaccharide is aminated on the terminal aldehyde group and exhibits (i) a carbon (13C) NMR spectrum having (a) no resonance signal between 13 and 14 ppm inclusive; (b) no resonance signal between 11.5 and 12.5 ppm, inclusive; and (c) a resonance signal located between 17 and 18 ppm inclusive, characteristic of N-acetylated quinovosamine, the intensity of which is increased in comparison with the resonance signal located between 17 and 18 ppm, inclusive, in the (13C) NMR spectrum of a pneumococcus type 5 capsular polysaccharide obtained after reductive amination in the presence of sodium cyanoborohydride at pH 8 for 48 hours; (ii) an HPAEC-PAD chromatogram obtained by elution from a anion-exchange column in an 18 mM sodium hydroxide solution at a flow rate of 1 ml/min for 15 min of monosaccharides derived from hydrolysis of said polysaccharide having: (a) no peak between fucosamine and pneumosamine peaks; and (b) a peak located immediately after the pneumosamine peak characteristic of quinovosamine, the intensity of which is increased in comparison with the equivalent peak in the HPAEC-PAD chromatogram of a pneumococcus type 5 capsular polysaccharide obtained after reductive amination in the presence of sodium cyanoborohydride at pH 8 for 48 hours; or (iii) both, and wherein the anion-exchange column consists of a support based on polystyrene and sulfonated divinylbenzene having a degree of cross-linking of 55% and latex microbeads with quaternary ammonium groups; wherein the latex microbeads have a degree of cross-linking of 5% and the diameter of 400 nm.