Patent ID: 8338180

Claim:
A genetically modified eukaryotic cell comprising: (i) an amount of a nucleic acid consisting of a nucleotide sequence encoding an APOBEC3G polypeptide and operably linked to a nucleic acid comprising regulatory nucleotide sequences; and (ii) an amount of a nucleic acid consisting of a nucleotide sequence encoding a Vif polypeptide and operably linked to a nucleic acid comprising regulatory nucleotide sequences; wherein the amount (i) and (ii) is selected to produce an amount of the APOBEC3G polypeptide and an amount of the Vif polypeptide in the cell so that the amount of the APOBEC3G polypeptide and the amount of the Vif polypeptide produced in the cell permit (a) detecting a Vif polypeptide-mediated reduction in the level of the APOBEC3G polypeptide in the cell; (b) detecting a reduced incorporation of the APOBEC3G polypeptide into a human immunodeficiency virus virion; or (c) detecting a shortening of the half-life of the APOBEC3G polypeptide.