Patent ID: 8653331

Claim:
A process for enhanced photobiological H 2 production using a non-regenerative transgenic alga, comprising the steps of: a) growing a transgenic alga having a first exogenous transgene for providing a photosystem-II (PSII) function switch wherein said first exogenous transgene is selected from the group consisting of OEE1 iRNA, D1 iRNA, CP47 iRNA, CP43 iRNA and D2 iRNA, and having a second exogenous transgene for inserting proton channels into algal plasma membranes; b) inducing the first exogenous transgene to increase photobiological H 2 productivity; c) producing H 2 and CO 2 with the induced transgenic alga by the metabolic and PSI-driven H 2 production pathways; and d) inducing the second exogenous transgene to prevent regeneration of the transgenic alga.