Patent ID: 7794943

Claim:
A method of detecting a mutation or polymorphism in a target oligonucleotide, comprising: attaching a first oligonucleotide analogue probe set to the array, wherein the probes of said first analogue probe set comprise at least one sugar moiety having a modified 2′ position, wherein said first probe set comprise at least one interrogation position, and wherein the oligonucleotides of said first probe set comprise a sequence which is perfectly complementary to the target oligonucleotide sequence; attaching a second oligonucleotide analogue probe set to the array, wherein the probes of said second analogue probe set comprise at least one sugar moiety having a modified 2′ position, wherein said second probe set is identical to the first probe set, except that the second probe set comprises a different nucleotide analogue than the first probe set at least one interrogation position; hybridizing the target oligonucleotide to the array; and detecting which probe set is hybridized with the target oligonucleotide, thereby detecting the mutation or polymorphism in the target oligonucleotide.