Patent ID: 8333953

Claim:
A method for measuring a cell mediated immune response, comprising contacting a cell with an isolated polypeptide comprising a target antigen and a transport factor, wherein the transport factor contains a fragment of at least 104 C-terminal amino acids of a bipartate protein exotoxin which is not toxic to the cell and functions to deliver the target antigen to the cytosol of the cell, and wherein a protective antigen (PA) of a bipartate exotoxin is not present, and measuring the increase in at least one cytokine or the increase in a cell surface antigen expressed on the cell in response to delivering the target antigen to the cytosol of the cell, wherein the increase of at least one cytokine or expression of a cell surface antigen by the cell, as compared to the corresponding at least one cytokine or expression of a cell surface antigen in the absence of the target antigen and transport factor, is indicative of a cell mediated immune response.