Patent ID: 7829505

Claim:
A process for the construction of oligonucleotide microarrays on glass or other polymer surfaces, which comprises (i) reacting 3′- or 5′-phosphorylated oligonucleotides with glycidyloxyropyltriethoxysilane (GOPTS) at an elevated temperature to form a conjugate immobilized on a surface of a solid support via route A or route B, (ii) removing any unreacted components from the solid support, and (iii) labeling the conjugate by hybridizing it with a labeled complementary oligomer sequence and visualizing the labeled conjugate with a laser scanner, wherein route A comprises mixing glycidyloxyropyltriethoxysilane (GOPTS) with the oligonucleotides to obtain the conjugate, by reacting the 3′ or 5′ phosphate with the epoxide, and then spotting the conjugate on the surface; and wherein route B comprises coating the surface with glycidyloxyropyltriethoxysilane (GOPTS), and then spotting the oligonucleotides on the glycidyloxyropyltriethoxysilane (GOPTS) coated surface wherein the 3′ or 5′ phosphate of the oligonucleotide reacts with the epoxide of GOPTS.