Patent ID: 8067191

Claim:
A method of detecting IL-16 biological activity in a sample comprising the steps of: a) providing a first population of eukaryotic cells surrounded by media and responsive to IL-16 biological activity with a first test sample; b) providing a second population, of the same type of eukaryotic cells as in a) and surrounded by media and responsive to IL-16 biological activity, with a negative control sample; c) measuring the amount of a RANTES proxy protein produced by the first and second populations of eukaryotic cells; and d) comparing the amount of a RANTES proxy protein produced by the first and second populations of eukaryotic cells, wherein a smaller amount of a RANTES proxy protein produced by the first population of eukaryotic cells relative to the RANTES proxy protein level produced by the second population of eukaryotic cells indicates the detection of IL-16 biological activity in the test sample.