Patent ID: 6962820

Claim:
A use of a fluorescent label in a particle comprising incorporating one or more fluorescent label into a particle, at least one fluorescent label being a compound of the formula: wherein: A and B each independently represent ring structures with sufficient carbon atoms to make up a cyanine nuclei; X and Y are each independently selected from the group consisting of O, S, NR 9 , and CR 9 R 10 ; R 1 and R 2 are each independently selected from the group consisting of H, C 1 -C 20 alkyl, C 1 -C 20 haloalkyl, C 1 -C 20 alkylene, and C 1 -C 20 haloalkylene; R 3 is selected from the group consisting of H, halogen, OH, OR 11 , SR 11 , NR 11 R 12 , C 1 -C 6 alkyl, C 1 -C 6 alkylene, C 3 -C 6 cycloalkyl, C 3 -C 6 cycloheteroalkyl, C 3 -C 6 cycloalkylene, C 3 -C 6 cycloheteroalkylene, phenyl, biaryl, heteroaryl, and heterobiaryl, wherein the C 1 -C 6 alkyl, C 1 -C 6 alkylene, C 3 -C 6 cycloalkyl, C 3 -C 6 cycloheteroalkyl, C 3 -C 6 cycloalkylene, C 3 -C 6 cycloheteroalkylene, phenyl, biaryl, heteroaryl and heterobiaryl groups are unsubstituted or substituted with halogen, OH, C 1 -C 4 alkyl, or C 1 -C 4 haloalkyl; R 8 is selected from the group consisting of C 1 -C 4 alkyl, and C 1 -C 4 haloalkyl; R 9 and R 10 are each independently selected from the group consisting of hydrogen, C 1 -C 4 alkyl, and C 1 -C 4 haloalkyl; and R 11 and R 12 are each independently selected from the group consisting of C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, phenyl, biaryl, heteroaryl, or heterobiaryl, wherein the C 1 -C 6 alky 1 , C 1 -C 6 cycloalkyl, phenyl, biaryl, hereroaryl, and heterobiaryl groups are unsubstituted or substituted with halogen, OH, C 1 -C 4 alkyl, or C 1 -C 4 haloalkyl, or when R 3 represents NR 11 R 12 , R 11 and R 12 may be taken together to form an optionally substituted C 3 -C 6 aliphatic or C 3 -C 6 aromatic heterocyclic ring.