Patent ID: 8247535

Claim:
An isolated variant glyphosate tolerant EPSP synthase polypeptide comprising SEQ ID NO:2, wherein said variant is a substitution mutation of two or more amino acids in said SEQ ID NO:2 selected from the group consisting of: i) a cysteine residue at the position corresponding to amino acid position 75 of SEQ ID NO:2; ii) an aspartic acid residue at the position corresponding to amino acid position 76 of SEQ ID NO:2; iii) an asparagine, alanine, glutamine, or threonine residue at the position corresponding to amino acid position 77 of SEQ ID NO:2; iv) a serine residue at the position corresponding to amino acid position 78 of SEQ ID NO:2; v) a serine or glycine residue at the position corresponding to amino acid position 81 of SEQ ID NO:2; vi) a valine, threonine, leucine, or phenylalanine residue at the position corresponding to amino acid position 82 of SEQ ID NO:2; vii) an alanine residue at the position corresponding to amino acid position 86 of SEQ ID NO:2; viii) a glycine residue at the position corresponding to amino acid position 87 of SEQ ID NO:2; ix) a valine, leucine, or histidine residue at the position corresponding to amino acid position 88 of SEQ ID NO:2; x) a serine, or threonine residue at the position corresponding to amino acid position 95 of SEQ ID NO:2; xi) a serine, arginine, glutamine, glutamic acid, or threonine residue at the position corresponding to amino acid position 206 of SEQ ID NO:2; and xii) a lysine, glutamine, arginine, serine, or threonine residue at the position corresponding to amino acid position 215 of SEQ ID NO:2.