Patent ID: 8547634

Claim:
An optical apparatus for photomanipulation of a sample comprising: a sample holder for receiving the sample; an illumination device comprising: an illumination light source; and an illumination beam path for illuminating the sample with a light sheet; a detection device for detecting light that is radiated from the sample; imaging optics which image the sample at least partially on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero; a control unit; means for photomanipulation of the sample, wherein the illumination light source at the same time is a first manipulation light source and forms a first means for photomanipulation, and wherein as a second means for photomanipulation, a second manipulation light source is provided, and light from the second manipulation light source is coupled into the imaging beam path via manipulation optics and deflected to the sample by the imaging objective.