Patent ID: 8053205

Claim:
An immunoassay for detecting doxorubicin in a sample comprising providing a mixture of a) said sample, b) an antibody selectively reactive with doxorubicin and not substantially cross-reactive with doxorubicin aglycone and c) a conjugate of a carrier having either a reactive thiol or amino group with a compound of the formula: wherein A is Y is an organic spacing group; X is a functional group capable of binding to said carrier through said amino or thiol group; and p is an integer from 0 to 1; or a compound of the formula: wherein X, Y and p are as above and B is —CH 2 — or or mixtures comprising a conjugate of said carrier with a compound of Formula IIA and a conjugate of said carrier with a compound of Formula IIB; causing the doxorubicin in the sample and said conjugate in said mixture to bind in said mixture with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of doxorubicin in the sample can be determined.