Patent ID: 8414760

Claim:
A method of making an electrochemical sensor, the method comprising: applying a plurality of electrodes onto the surface of a substrate; applying a conductive ink to one or more of the plurality of electrodes, wherein the conductive ink comprises an analyte-responsive enzyme and a mediator compound wherein the mediator compound is one of the following two formulae: where X and Y can independently be oxygen, sulphur, CR 3 R 4 , NR 3 , or NR 3 R 4+ or the functional group CZ 1 Z 2 , where Z 1 and Z 2 are electron withdrawing groups: R 1 and R 2 can independently be a substituted or unsubstituted aromatic or heteroaromatic group; and R 3 and R 4 can independently be a hydrogen atom, a hydroxyl group or a substituted or unsubstituted alkyl, aryl, heteroaryl, amino, or aryloxyl group; applying a dielectric layer on top of the plurality of electrodes, wherein the dielectric layer covers at least a portion of the plurality of electrodes.