Patent ID: 8765455

Claim:
An apparatus for sorting droplets on a chip, consisting of: a chip; a microfluidic flow channel in said chip; a carrier fluid in said microfluidic flow channel; a droplet maker connected to said microfluidic flow channel that provides a flow stream of said carrier fluid and said droplets in said microfluidic flow channel wherein said droplets have substantially the same diameter and wherein said droplets include first droplets containing genetic material and second droplets that do not contain said genetic material; an amplifier connected to said microfluidic flow channel for amplifying said genetic material in said first droplets containing genetic material producing first amplified droplets containing amplified genetic material and second droplets that do not contain said genetic material; an analyzer connected to said microfluidic flow channel; a waste channel connected to said microfluidic flow channel; and an alternating current dielectrophoresis droplet sorter connected to said microfluidic flow channel that sorts said droplets according to said first amplified droplets containing said amplified genetic material and directs said first amplified droplets containing said amplified genetic material to said analyzer and sorts said droplets according to said second droplets that do not contain said genetic material and directs said second droplets that do not contain said genetic material to said waste channel, said alternating current dielectrophoresis droplet sorter including inter-digitated electrodes that produce a field gradient in said flow channel and on said first amplified droplets containing said amplified genetic material and said second droplets that do not contain said genetic material, and a controller connected to said inter-digitated electrodes that varies said field gradient and diverts said first amplified droplets containing said amplified genetic material to said analyzer, and diverts said second droplets that do not contain said genetic material to said waste channel.