Patent ID: 7955625

Claim:
A method for the production of a canola protein isolate consisting predominantly of the 2S canola protein, which comprises: extracting canola oil seed meal with an aqueous salt solution to extract canola proteins from the canola oil seed meal and to obtain a canola protein solution, separating the canola protein solution from residual oil seed meal, concentrating said canola protein solution to provide a concentrated protein solution as a retentate, heat treating the concentrated protein solution at a temperature of about 70° to about 100° C. for about 2 to about 30 minutes to selectively precipitate 7S and 12S canola proteins, in preference to 2S canola protein, from the concentrated canola protein solution to reduce the 7S and 12S protein contents by at least about 50 wt % and to form a heat-treated canola protein solution containing predominantly 2S canola protein, separating the heat-treated canola protein solution from the precipitated proteins, and recovering a canola protein isolate having a protein content of at least about 90 wt % (N×6.25) and consisting predominantly of 2S canola protein from the separated heat-treated canola protein solution.