Patent ID: 6991903

Claim:
A method for sequencing a target nucleic acid comprising the steps of: (a) providing a set of partially single-stranded nucleic acid fragments wherein each fragment contains a sequence that corresponds to a sequence of the target; (b) hybridizing the single-stranded portions of the fragments to single-stranded portions of a set of partially double-stranded nucleic acid probes to form a set of complexes, wherein each probe contains a variable sequence within the single-stranded region, and for each complex; (i) ligating a single strand of the fragment to an adjacent single strand of the probe; and (ii) extending the unhigated strand of the complex by strand displacement polymerization using the ligated strand as a template; and (c) determining the molecular weights of the extended strands; and (d) determining the sequence of the target from the molecular weights of the extended strands, wherein the extended strand contains mass-modifying functionalities.