Patent ID: 7947819

Claim:
An isolated nucleic acid that hybridizes under stringent hybridization conditions to SEQ ID NO: 2, wherein the nucleic acid is the complement of a polynucleotide that encodes a variant B-Raf polypeptide having kinase activity and comprising Glu or Asp at the position corresponding to position 599 of SEQ ID NO: 1, wherein said stringent hybridization conditions comprise an overnight incubation at 42° C. in a solution comprising 50% formamide, 5X SSC (750 mM NaCl, 75 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5X Denhardt's solution, 10% dextran sulphate, and 20 pg/ml denatured, sheared salmon sperm DNA, followed by washing the filters in 0.1X SSC at 65° C.