Patent ID: 7262168

Claim:
A composition having a pH of about pH 5.5 or greater, wherein said composition comprises: (a) biologically active insulin-like growth factor-1 (IGF-I) or biologically active analogue thereof having an amino acid sequence that shares at least 70% sequence identity with the amino acid sequence for human IGF-I, wherein said IGF-I or analogue thereof is present at a concentration of about 12 mg/ml to about 200 mg/ml when said composition is at a temperature of about 4° C.; and (b) a solubilizing compound selected from the group consisting of guanidine, guanidine acetate, guanidine carbonate, guanidine 1-cyano, guanidine 1,3-diphenyl, guanidine 1,3-di(2-toyl), guanidine hydrochloride, guanidine nitrate, 1-nitroguanidine, guanidine picrate, guanidine thiocyanate, guanidine tetraphenyl, guanidine 1,1,3-triphenyl, guanidine 1,2,3-triphenyl, agmatine, 4-guanidinobenzoic acid, guanidinoacetic acid, guanidinosuccinic acid, guanethidine, 4′-acetamidophenyl 4-guanidinobenzoate, 2-iminobiotin, N-(2-guanidinoethyl)-5-isoquinolinesulfonamide, guanidinobutyric acid, and guanidinopropionic acid, wherein said solubilizing compound is present in said composition in an amount sufficient to make said IGF-I or analogue thereof soluble at a concentration of about 12 mg/ml to about 200 mg/ml when said composition is at a temperature of about 4° C.