Patent ID: 8288522

Claim:
A composition comprising: a first target nucleic acid; a nucleic acid comprising a first surrogate nucleic acid; a first set of two or more surrogate capture probes, each of which surrogate capture probes is capable of hybridizing simultaneously to the first target nucleic acid and to the nucleic acid comprising the first surrogate nucleic acid, wherein the 5â€² end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 3â€² end hybridizes to the nucleic acid comprising the first surrogate nucleic acid, or wherein the 3â€² end of each of the surrogate capture probes in the first set hybridizes to the first target nucleic acid while the 5â€² end hybridizes to the nucleic acid comprising the first surrogate nucleic acid; a solid support; a first set of m target capture probes, where m is at least one, which target capture probes are capable of hybridizing to the first target nucleic acid; and one or more primers for amplifying the first surrogate nucleic acid.