Patent ID: 8865966

Claim:
A method for producing pentameric canine C-reactive protein (CRP), said method comprising the steps of: (a) transforming a first silkworm with an expression vector comprising the nucleic acid sequence as set forth in SEQ ID NO: 4 encoding monomeric canine CRP (mCRP) operably linked to a UAS sequence, (b) transforming a second silkworm with an expression vector comprising a nucleic acid sequence encoding GAL4 operably linked to a silk gland-specific promoter, (c) mating the silkworms from steps (a) and (b) to obtain a transgenic offspring whose genome comprises (i) the nucleic acid sequence as set forth in SEQ ID NO: 4 encoding canine mCRP operably linked to a UAS sequence and (ii) a nucleic acid sequence encoding GAL4 operably linked to a silk gland-specific promoter, wherein said transgenic offspring express mCRP; and (d) obtaining pentameric canine CRP from the silk gland from the transgenic silkworm offspring in step (c).