Patent ID: 7056728

Claim:
A vector comprising, (a) a vector backbone comprising at least one polynucleotide sequence encoding light generating polypeptide sequences operably linked to a promoter functional in a target organism of interest and (b) a transposon cassette comprising a polynucleotide sequence comprising an internal polynucleotide sequence, said internal polynucleotide sequence comprising (i) a first sequence of interest encoding at least one light generating polypeptide sequence, said first sequence present in a first orientation, capable of being expressed in a gram-positive target organism and lacking control sequences that are capable of promoting transcription in the target organism and (ii) a transposase coding sequence operably linked to a promoter functional in the target organism, wherein said transposase coding sequence is in a second orientation relative to polypeptide coding sequences of the first sequence of interest encoding polypeptide sequences, and said transposase is capable of inducing transposition mediated by transposon inverted repeats; and first and second transposon inverted repeat sequences, wherein said first and second transposon inverted repeat sequences (i) are from a gram-positive bacterium; and (ii) flank said internal polynucleotide sequence; wherein said promoter in said vector backbone does not affect transcription of any coding sequences in the transposon cassette.