Patent ID: 6924096

Claim:
A method for suppression of gene expression in a cell comprising: (a) administering to the cell in vitro a suppressive-effective amount of a nucleic acid construct comprising in 5′ to 3′ operable orientation: (i) a 5′ stem loop structure; (ii) an antisense nucleic acid; and (iii) a 3′ stem loop structure, wherein the antisense nucleic acid suppresses gene expression and is flanked by the stem loop structures and with the proviso that the antisense nucleic acid is not within the 5′ or 3′ stem loop structures of the construct; and (b) administering a modified nucleic acid encoding a wild-type polypeptide corresponding to the gene product of the gene being suppressed, wherein the modified nucleic acid is resistant to ribozyme cleavage and/or antisense inhibitions, whereby expression of the gene is suppressed in the cell.