Patent ID: 8765435

Claim:
A thermostable, family A-type DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the DNA polymerase comprises a motif in the polymerase domain comprising V-D-P-L-M-R—R-A-X 6 —K-T-V—N—F, wherein: X 6 is any amino acid other than A or G (SEQ ID NO: 10); wherein the DNA polymerase has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:1, 2, 3, 4, 5, 6, or 7, and wherein the control DNA polymerase has the same amino acid sequence as the DNA polymerase except that the amino acid of the control DNA polymerase at position X 6 is A or G.