Patent ID: 7201899

Claim:
A method for generating a porcine cell comprising at least one inactivated α-1,3 galactosyltransferase gene, the method comprising: (a) providing a plurality of porcine cells; (b) introducing, in vitro, into said cells a DNA construct comprising a disrupted porcine α-1,3 galactosyltransferase gene, wherein the disruption is by insertion of an exogenous sequence into said gene such that disruption prevents expression of functional α-1,3 galactosyltransferase, wherein the gene, prior to disruption, encodes a porcine α-1,3 galactosyltransferase with the amino acid sequence of SEQ ID NO:10; (c) incubating said cells such that homologous recombination occurs between the chromosomal sequence encoding α-1,3 galactosyltransferase and the introduced DNA construct comprising the disrupted α-1,3 galactosyltransferase gene; and (d) identifying a porcine cell comprising at least one inactivated α-1,3 galactosyltransferase gene, wherein the gene, prior to disruption, encodes a porcine α-1,3 galactosyltransferase with the amino acid sequence of SEQ ID NO:10.