Patent ID: 8148110

Claim:
A method for determining the occurrence of binding between a first and a second putative binding moiety in a eukaryotic cell, the method comprising: a) providing a beta-lactamase enzyme complementation reporter system comprising: a first component comprising a first beta-lactamase enzyme fragment coupled to the first putative binding moiety wherein the first beta-lactamase enzyme fragment is about 19 kDa in size; and a second component comprising a second beta-lactamase enzyme fragment coupled to the second putative binding moiety wherein the second beta-lactamase enzyme fragment is about 10 kDa in size; wherein the first beta-lactamase enzyme fragment and the second beta-lactamase fragment are complementing beta-lactamase enzyme fragments, wherein beta-lactamase enzyme complementation occurs when the first putative binding moiety binds to the second putative binding moiety; b) combining the first component and the second component in a eukaryotic cell; and c) detecting beta-lactamase enzyme complementation in the eukaryotic cell, wherein detection of beta-lactamase enzyme complementation and resultant beta-lactamase enzymatic activity indicates the occurrence of binding between the first and second binding moieties in the eukaryotic cell.