Patent ID: 7183406

Claim:
A method for the fabrication of a DNA construct of a target sequence comprising the steps of (a) defining a plurality of overlapping single stranded DNA sequence segments which in the aggregate would make up the target DNA sequence, wherein the single stranded segments overlap in the regions where the segments are complementary to each other; (b) making a DNA microarray on a substrate, the microarray made up of features having single stranded DNA molecules, the single stranded DNA molecules selected to be overlapping sequence segments of the target sequence, the single stranded DNA molecules being made attached by linkers to the substrate, the linkers being stable under the conditions of DNA synthesis for the microarray, the linkers also being capable of being rendered photo-labile by chemical treatment; (c) detaching the single stranded DNA molecules from the substrate by first applying a chemical modification to the linkers to render the linkers light labile and then by selectively applying light to cleave the labile linkers in areas of single stranded DNA molecules thus detaching the single stranded DNA molecules from the substrate; and (d) allowing the single stranded DNA molecules detached in step (c) to assemble into a double stranded target DNA construct by providing conditions favoring hybridization of the single stranded DNA molecules to each other using the 3′ overhang contained on either strand.