Patent ID: 7785795

Claim:
A method of quantifying at least one nucleic acid, which comprises: conducting a nucleic acid polymerization reaction in a nucleic acid polymerization reaction system that is a reaction solution comprising: (A) said at least one nucleic acid as a template, (B)(a) a fluorescent dye capable of emitting fluorescence upon binding to a nucleic acid; and (b) a nucleotide monomer labeled with an acceptor fluorescent dye, and (C) at least one nucleic acid-synthesizing enzyme, measuring in real-time during the nucleic acid polymerization a change or an amount of a change in an optical character of said nucleic acid polymerization system as the reaction solution; and determining the quantity of said template nucleic acid or nucleic acid, being synthesized using said template nucleic acid as a template based on the measured change or the measured amount of the change in the optical character.