Patent ID: 7273705

Claim:
An mRNA separation method, comprising the steps of: (a) injecting a reaction solution into a reaction region which contains a covalent linking of a tRNA probe and a covalent linking of an rRNA probe for hybridizing tRNA and rRNA in the reaction solution; (b) adjusting a moderate hybridization temperature in the reaction region to hybridize the tRNA and the rRNA in the reaction solution; (c) moving the reaction solution in the reaction region after hybridization to a transition region by a pneumatic actuator; (d) adding a buffer solution to the reaction region, and heating the reaction region to a moderate denature temperature such that the tRNA and the rRNA hybridized by the tRNA probe and the rRNA probe depart from the tRNA probe and rRNA probe into the buffer solution; (e) moving the buffer solution with the departed tRNA and rRNA in the reaction region after denature step (d) to a waste region by the pneumatic actuator to discard the departed tRNA and rRNA; (f) moving the reaction solution in the transition region in the step (c) back to the reaction region by the pneumatic actuator; repeatedly performing the steps (a) through (f) so as to purify mRNA in the reaction solution; wherein the steps (a) through (f) are performed on a microfluidic chip.