Patent ID: 8597935

Claim:
A particle-sorting method using acridine orange to sort cells in blood, comprising the steps of: staining cells in the blood with acridine orange; irradiating light on the cells in the blood; receiving the irradiated light with detectors; measuring the sizes of red blood cells and platelets by detecting forward scatter (FS) using a detector for small-angled forward-scattered light; measuring the amount of granules by detecting side scatter (SS) using a detector for lateral-scattered light; measuring the amount of DNA by detecting a first fluorescence (FL 1 ) using a first fluorescence detector; measuring the amount of RNA by detecting a second fluorescence (FL 2 ) using a second fluorescence detector; determining the intracellular DNA concentration and intracellular RNA concentration by dividing the amount of DNA (FL 1 ) and the amount of RNA (FL 2 ) by the measured sizes of red blood cells and platelets (FS) or the measured amount of granules (SS); constructing a scattergram of DNA concentration and RNA concentration; and sorting the cells in the blood using the scattergram.