Patent ID: 8518417

Claim:
A modified Clostridial botulinum toxin comprising: a) a Clostridial botulinum toxin type A enzymatic domain; b) a Clostridial botulinum toxin type A translocation domain; c) a translocation facilitating domain comprising an enveloped virus fusogenic peptide domain capable of facilitating a translocation step of a Clostridial toxin intoxication process; and, d) an enhanced targeting domain consisting essentially of a modified Clostridial botulinum H CC targeting domain capable of executing a cell binding step of a Clostridial botulinum toxin intoxication process; wherein the modified Clostridial botulinum toxin comprises a linear amino-to-carboxyl order of the Clostridial botulinum toxin enzymatic domain, the Clostridial botulinum toxin translocation domain, the translocation facilitating domain and the enhanced targeting domain, and wherein the modified Clostridial botulinum H CC targeting domain comprises a Clostridial botulinum serotype A NTNH β-fold and exhibits enhanced binding activity for an endogenous Clostridial botulinum toxin receptor relative to the binding activity of a naturally-occurring Clostridial botulinum serotype A H CC targeting domain.