Patent ID: 7303913

Claim:
A method for detecting a Peste des Petits Ruminants virus (PPRV) N-protein antibody in a sample comprising (a) providing a surface which is coated with an N-protein of PPRV; (b) contacting the surface with a labeled PPRV N protein antibody, said labeled PPRV N protein antibody being produced by a hybridoma P3H12 (KCLRF-BP-00104), wherein the contact of the surface and the labeled PPRV N protein antibody is carried out in the presence of the sample that contains the PPRV N protein antibody, and in the absence of the sample that contains the PPRV N protein antibody, respectively; (c) assaying to determine the quantity of the labeled PPRV N protein antibody bound to the surface, each in the presence of the sample that contains the PPRV N protein antibody, or in the absence of the sample that contains the PPRV N protein antibody; and (d) comparing the quantity of the labeled PPRV N protein antibody bound to the surface in the presence of the sample with the quantity of the labeled PPRV N protein antibody bound to the surface in the absence of the sample, wherein said N-protein of PPRV is a recombinant protein produced by expressing a recombinant baculovirus plasmid harboring the PPRV N-protein in an insect cell, said recombinant baculovirus plasmid having Nar1 and Sac1 restriction sites.