Patent ID: 7226752

Claim:
A method of detecting the presence of an analyte in a sample, said method comprising: mixing said sample with a chemiluminescent indirectly-detectable labeled binding member comprising a label moiety to produce a first composition that includes binding complexes comprising said labeled binding member; separating said unbound labeled binding member from binding complexes present in said first composition to produce a second composition comprising said binding complexes; adding a light wavelength converter and an oxidant to the second composition; detecting said binding complexes in said second composition to detect the presence of said analyte in said sample, wherein said detecting comprises detecting second light emitted by said light wavelength converter, said light wavelength converter generating said second light in response to absorbing first light generated by said indirectly-detectable labeled binding member of said binding complexes of said second composition, said first light being generated in response to reaction of said indirectly-detectable labeled binding member with said oxidant.