Patent ID: 7105299

Claim:
A kit for determining a haplotype of specimen chum salmon, which comprises an oligonucleotide-immobilized substrate obtained by immobilizing, on a substrate, one or more oligonucleotides that enable detection of a combination of polymorphisms selected from the group consisting of (2) 96D, T10C; (3) 96D, A42G; (4) 96D, A108C; (12) 96D, 386D, C395A, C154G, T10C; (13) 96D, 386D, C395A, C154G, T7OC; (14) 96D, 386D, C395A, C154G, A108C; (15) 96D, 386D, C395A, C154G, T231C; (16) 96D, 386D, C395A, C154G, C242T; (17) 96D, 386D, C395A, C154G, T250C; (18) 96D, 386D, C395A, C154G, A260G; (19) 96D, 386D, C395A, C154G, T339A; (20) 96D, 386D, C395A, C154G, T401C; (21) 96D, 386D, C395A, C154G, A471C; (22) 96D, 386D, C395A, C154G, T339A, C340T; (23) 96D, 386D, C395A, C154G, T339A, T401C; and (26) 96D, T30C, T70C, in a nucleotide sequence of mitochondrial DNA control region of chum salmon by hybridization, wherein the immobilized oligonucleotide comprises a position selected from the 10th, 30th, 42nd, 70th, 96th, 108th, 154th, 231st, 242nd, 250th, 260th, 339th, 340th, 386th, 395th, 401st and 471st positions of SEQ ID NO: 8, and determining the haplotype based on the combination of polymorphisms detected by hybridization of the oligonucleotides with a nucleic acid derived from the specimen chum salmon.