Patent ID: 8343776

Claim:
A method for releasing, from a surface of a base plate, immunoglobulin G antibody bound to the surface via protein A, the method comprising the following steps (A) and (B): a step (A) of preparing the base plate having the surface to which immunoglobulin G antibody is bound via protein A by supplying a buffer solution containing sodium chloride and the immunoglobulin G antibody to the surface of the base plate where the protein A has been immobilized; wherein the concentration of the sodium chloride contained in the buffer solution is not less than 4 g/liter and not more than 16 g/liter, and a step (B) of providing a solution containing human serum albumin onto the surface so as to release the immunoglobulin G antibody from the protein A, wherein the immunoglobulin G antibody is an anti-human albumin monoclonal antibody 2F13F11G11 produced by a producer cell line of deposit No. FERM BP-10459, the solution has a pH not lower than 6 and not higher than 8.9, and in the step (B), a complex of the immunoglobulin G antibody and the human serum albumin is formed through antigen-antibody reaction.