Patent ID: 7324200

Claim:
A fluorescence photometric apparatus comprising at least: a light source; a light irradiating unit configured to condense light from the light source on a sample by means of an objective lens and irradiating the sample with the condensed light; and a photodetector which detects fluorescence emitted from the sample, wherein the fluorescence photometric apparatus includes: an illuminating unit configured to obtaining a sample image; a position adjusting unit configured to adjusting a relative position of the sample and a position of a light spot condensed by the light irradiating unit; and an imaging unit configured to simultaneously two-dimensionally or three-dimensionally imaging both the sample image and an image of the light spot from the light irradiating unit configured to condensing the light on the sample, wherein a reflecting unit is provided in an optical path extending from the objective lens to the photodetector, and the reflecting unit at least partially reflects both light forming the sample image and light from the light source transmitted through, reflected by or scattered by the sample so that the lights travel toward the imaging unit, and wherein the reflecting unit includes a partially transmitting optical element or a beam splitter having wavelength characteristics.