Patent ID: 7122333

Claim:
A method for visually measuring a level of ATP in a sample comprising: i) forming glucose-6-phosphate in the presence of the ATP in the sample with, acetyl phosphate and glucose, and by a reaction of a coupled enzyme reaction system comprising an acetate kinase enzyme and one of a glucokinase enzyme or a hexokinase enzyme; ii) changing a color state of a chromogen subsequently in response to the amount of formed glucose 6-phosphate, by a reaction of another coupled enzyme reaction system comprising: a glucose-6-phosphate dehydrogenase enzyme and a diaphorase enzyme; or a glucose-6-phosphate dehydrogenase enzyme and an electron carrier, in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate and in the presence of a chromogen, and iii) determining the level of ATP in the sample by measuring the change in the color state of said chromogen.