Patent ID: 8304204

Claim:
A method for measuring low-density lipoprotein (LDL) cholesterol in a sample using a test piece comprising a total-cholesterol measurement portion, which comprises an enzyme reagent for measuring cholesterol, and a non-LDL cholesterol measurement portion, which comprises an enzyme reagent for measuring cholesterol, a surfactant for solubilizing non-LDL cholesterol, a surfactant for inhibiting LDL cholesterol dissolution, and a LDL cholesterol agglomerating agent, the method comprising steps (A) to (C): (A) measuring total cholesterol in the sample in the total-cholesterol measurement portion of the test piece; (B) measuring selectively non-LDL cholesterol in the sample in the non-LDL cholesterol measurement portion of the test piece; and (C) obtaining an LDL cholesterol level of the sample by subtracting the non-LDL cholesterol value measured in the step (B) from the total-cholesterol value measured in the step (A), wherein a portion for measuring total cholesterol and a portion for measuring non-LDL cholesterol are provided on the same test piece; and wherein in the step (B), the non-LDL cholesterol is selectively measured by the enzyme reagent for measuring cholesterol, wherein the surfactant for solubilizing non-LDL cholesterol solubilizes the non-LDL cholesterol, the surfactant for inhibiting LDL cholesterol dissolution inhibits LDL cholesterol dissolution and the LDL cholesterol agglomerating agent agglomerates the LDL cholesterol.