Patent ID: 8536314

Claim:
A recombinant fluorescent protein having a light, oxygen, voltage domain (LOV domain) in which at least one cysteine is replaced by another amino acid that does not covalently bind FMN, characterized in that the LOV domain is an amino acid sequence selected from the group consisting of SEQ ID NO: 1, 2, 3 or 4, wherein the LOV domain comprises the substitution of the at least one cysteine and at least one further point mutation for changing the fluorescence wavelength, selected from the group consisting of Y112H, I48T, H61Y, Y43F, Y112C, E12D, Q143L, A36T, Q57H, N951, E22K, E71G, K88S, L109V and Q116L, and wherein the numbering of said point mutation is based on the amino acid sequence of SEQ ID NO: 4.