Patent ID: 6861259

Claim:
A method of sealing a chamber to preclude air entrapment in a test liquid, comprising: (a) providing an analytical chamber having a test chamber and an overflow reservoir; (b) filling the analytic chamber with said test liquid to a level below a fill line; (c) adding a buffer solution having a density not more than the density of the test liquid with a combined volume being sufficient to bring said level of said combined liquids to said fill line; (d) seating a primary seal within the analytic chamber to form a seal interface that lies between the test chamber and the overflow reservoir within the liquid volume in said analytic chamber, whereby all of said test liquid is retained in said chamber, and air is excluded from said chamber; (e) retaining any evacuated liquid displaced upon seating said primary seal into said overflow reservoir, and (f) sealing the overflow with a second seal to preclude voiding of said overflow reservoir and rupturing of said primary seal.