Patent ID: 7960605

Claim:
A method of testing a Caloric Restriction (CR) mimetic or a CR mimetic candidate, the method comprising: selecting at least one of the CR mimetic or the CR mimetic candidate comprising a plant extract; administering the selected at least one of the CR mimetic or the CR mimetic candidate; determining antioxidant capabilities of the at least one of the CR mimetic or the CR mimetic candidate, wherein the determining includes determining: (i) a half maximal inhibitory concentration (IC 50 ) of the CR mimetic or the CR mimetic candidate having a dose dependent scavenging effect on a hydroxyl radical is consistent with a scavenging effect of a radical scavenger made by combining vitamin C and vitamin E, (ii) an IC 50 of the CR mimetic or the CR mimetic candidate having a dose dependent scavenging effect on a superoxide radical is consistent with a scavenging effect of a known superoxide radical scavenger and (iii) an IC 50 of the CR mimetic or the CR mimetic candidate having a dose dependent scavenging effect on a lipid radical is consistent with a scavenging effect of a known lipid radical scavenger; and determining in a subject a level of a biological parameter of a known CR marker by comparing the level of the known CR marker to measurements of a corresponding CR marker from a subject having been administered at least one of the CR mimetic or the CR mimetic candidate, the biological parameter of the known CR marker includes an RNA transcript level of a gene known to be effected in gene expression by a CR diet or a known CR mimetic.