Patent ID: 8293725

Claim:
A method for capturing and/or detecting prostate-specific membrane antigen (PSMA) presenting cells, comprising contacting the cells with a compound of the formula wherein each n is independently 1, 2, 3, 4, 5 or 6; each R 1 and R 2 are independently —C(O)OR 3 , —C(O)N(R 3 ) 2 , —P(O)(OR 3 ) 2 , —OP(O)(OR 3 ) 2 , —S(O) 2 R 3 , —S(O) 2 OR 3 , —S(O) 2 N(R 3 ) 2 , or tetrazolyl; each R 3 is independently —H or C 1 -C 6 alkyl; R 4 is —H, —C(O)OR 3 , —C(O)N(R 3 ) 2 , —P(O)(OR 3 ) 2 , —OP(O)(OR 3 ) 2 , —S(O) 2 R 3 , —S(O) 2 OR 3 , —S(O) 2 N(R 3 ) 2 , or tetrazolyl; L is —P(O)(OR 3 )— or —P(O)(N(R 3 ) 2 )—; M is —O—, —S—, —N(R 3 )—, or —CH 2 —; T is —O—, —S—, or —N(R 3 )—; R 7 is —X—R 8 or -L 1 -R 8 , wherein X is —O—, —S—, or —N(R 3 )—; L 1 is —C(O)N(R 3 )—, —C(S)N(R 3 )—, —C(O)CH(R 21 )—, —C(O)(O)—, —C(O)-L 2 -, a peptide, dendrimer, or peptide dendrimer, wherein R 21 is —H, aryl, heteroaryl, C 1 -C 6 alkyl-aryl optionally substituted with —OH; C 1 -C 6 alkyl-Heteroaryl, or C 1 -C 6 alkyl optionally substituted with —OR 23 , —SR 23 , —NH 2 , —N(H)C(═NH)NH 2 , —COOR 23 , or —C(O)N(R 23 ) 2 ; and R 23 is —H, C 1 -C 6 alkyl, or C 1 -C 6 alkyl-aryl; L 2 is —C 1 -C 24 alkyl- or -phenyl-C 1 -C 24 alkyl-, wherein each alkyl group is optionally substituted with 1 to 4 groups which are oxo, ═S, or —COOH; and one to six of the methylene groups in each alkyl group is optionally replaced by —O—, —S—, or —N(R 3 )—, provided that no two adjacent methylene groups are both replaced by —O—, —S—, or —N(R 3 )—; and R 8 is a detectable label or a biomolecular anchor linked to a solid support; and Q is —O—, —S—, —N(R 3 )—, —N(R 3 )O—, —ON(R 3 )—, or ═NO—; and when R 8 is a detectable label, detecting the detectable label under conditions that permit detection of only PMSA-presenting cells; or when R 8 is a biomolecular anchor linked to a solid support, capturing the PSMA-presenting cells.