Patent ID: 8062875

Claim:
An isolated polypeptide having perhydrolysis activity and being structurally classified as a carbohydrate esterase family 7 enzyme, said polypeptide having at least 95% amino acid sequence identity to SEQ ID NO: 10, provided that a substitution to amino acid 277 of SEQ ID NO: 10 is selected from the group consisting of serine, threonine, valine, and alanine, wherein said polypeptide has improved activity for production of an efficacious concentration of percarboxylic acid for disinfection relative to wild-type Thermotoga maritima acetyl xylan esterase of SEQ ID NO:36; improved activity across the entire pH range of activity relative to wild-type Thermotoga maritima acetyl xylan esterase of SEQ ID NO:36; improved perhydrolysis/hydrolysis ratio relative to wild-type Thermotoga maritima acetyl xylan esterase of SEQ ID NO:36; or a combination thereof.