Patent ID: 8563297

Claim:
A method of changing the affinity of a pair of chemical entities having a pH-dependent affinity, wherein the pair of chemical entities constitutes an affinity pair being a pair of two molecules that are capable of mutually bind non-covalently with high specificity and dissociate, the method comprising: a) immobilizing a first member of said pair of chemical entities having a pH-dependent affinity to a surface of an electrode; b) immersing said first member of said pair of chemical entities in an electrolysable environment so that said electrode is in electrical contact with said environment where a second member of said pair of chemical entities is found in said environment; c) making electrical contact between a counter electrode and said environment; and d) changing a potential between said electrode and said counter electrode so as to substantially change electrolysis of said environment thereby changing said affinity of said chemical entities so as to bind or dissociate reversibly by substantially changing a concentration of electrolysis products in said environment in proximity of said surface of said electrode.