Patent ID: 8548570

Claim:
A method for assessing vasculature of a tissue comprising: preprocessing hyperspectral or multispectral data by removing background radiation, the hyperspectral or multispectral data comprising a plurality of images of the tissue, each respective image in the plurality of images acquired at a different wavelength; (A) defining a region of interest in the tissue; (B) converting hyperspectral or multispectral intensities in the hyperspectral or multispectral data into units of optical density; (C) decomposing a spectra for each pixel of the converted image into several components; (D) defining three hyperspectral or multispectral data planes, each respective hyperspectral or multispectral data plane corresponding to a spectral property of the region of interest, the three planes consisting of hue, saturation, and value/intensity components of a pseudo-color hue-saturation-value/intensity (HSV/I) image of the region of interest, respectively; (E) adjusting color resolution of the pseudo-color HSV/I image according to an apparent concentration of one of oxygenated Hb, deoxygenated Hb, total Hb, or a mathematical combination thereof; (F) converting the adjusted pseudo-color HSV/I image to an RGB pseudo-color image; and (G) displaying the RGB pseudo-color image.