Patent ID: 8084216

Claim:
A method of determining whether a donor tissue intended for transplant or transfusion will induce transfusion related acute lung injury (TRALI) or graft versus host disease (GVHD) in a human recipient wherein the human recipient expresses the HNA-3a antigen comprising the amino acid sequence of SEQ ID: 1 or antigenic fragment thereof, the method comprising a) obtaining a sample from the donor of the tissue intended for transplant or transfusion in the human subject, wherein the sample is blood, blood derivative, plasma or serum, b) contacting the sample with a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or an antigenic fragment thereof to form a complex with HNA-3a specific antibodies in the sample, and c) detecting the complex, wherein the presence of the complex indicates that the donor tissue is likely to induce TRALI or GVHD in a human recipient that expresses the HNA-3a antigen.