Patent ID: 8278109

Claim:
A device for selectively detecting hyperglycosylated human chorionic gonadotropin (hCG-H) in a liquid sample deposited directly or indirectly on a proximal portion of the device for transport to a distal portion of the device, wherein the device comprises: A) a release medium formed of a first material and comprising a labeled conjugate with a detectable label and a first binding member that is reactive with at least one of an epitope of hCG-H or an epitope of human chorionic gonadotropin (hCG); and B) a capture medium in fluid communication with the release medium and formed of a second, different material, the capture medium comprising a capture site that comprises a second binding member immobilized on the capture medium, said second binding member being reactive with at least one of an epitope of hCG-H or an epitope of hCG; wherein at least one of the release medium and the capture medium comprises a binding member that exhibits a moderate to high affinity for hCG-H and is selectively or preferentially reactive with hCG-H, and wherein it is only the second binding member that exhibits the moderate to high affinity for hCG-H and is selectively or preferentially reactive with hCG-H.