Patent ID: 7985564

Claim:
An isolated nucleic acid sequence comprising a secretion signal coding sequence for a secretion peptide, wherein the secretion signal coding sequence is operably linked to a nucleotide sequence encoding a recombinant protein or polypeptide of interest, wherein the secretion signal is not native to the recombinant protein or polypeptide of interest, and wherein said secretion signal coding sequence comprises a nucleotide sequence selected from the group consisting of: a) the phosphate binding protein (pbp) secretion signal set forth in SEQ ID NO: 2; b) the Outer Membrane Porin E (OprE) secretion signal set forth in SEQ ID NO: 4; c) the Lys-Arg-Orn binding protein secretion signal set forth in SEQ ID NO: 8; d) the azurin secretion signal set forth in SEQ ID NO:6 e) the iron (III) binding protein secretion signal set forth in SEQ ID NO: 10; f) the lipoprotein B secretion signal set forth in SEQ ID NO:12; and, g) a nucleotide sequence encoding an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 1, 3, 7, 5, 9, or 11, wherein said nucleotide sequence encodes a secretion peptide.