Patent ID: 8182671

Claim:
A method for determining a concentration of an analyte in a sample, comprising the steps of: contacting a sample with a sensor, wherein the sensor comprises: a first electrode, a second electrode, and a third electrode; a sample chamber for holding the sample fluid, the sample chamber comprising the first electrode, the second electrode, and the third electrode, wherein the first electrode is positioned upstream of the second electrode relative to a sample entry port and the third electrode is positioned downstream of the second electrode relative to the sample entry port; and an analyte-responsive enzyme and a redox mediator disposed in the sample chamber; determining when the sample contacts the first electrode and the second electrode; determining when the sample contacts the first electrode and the third electrode; applying a potential between the first and second electrodes to electrolyze the analyte, thereby generating an analyte signal; and determining the concentration of the analyte using the analyte signal.