Patent ID: 8818069

Claim:
A method for scaling an image taken at a given exposure time to a selected exposure time, comprising, determining a dark pixel intensity of an imaging device, wherein determining the dark pixel intensity comprises, setting an exposure time to zero, wherein the exposure time comprises a time interval over which pixel intensity signals are read from the imaging device; acquiring an intensity image; and calculating a mean intensity for the entire intensity image; acquiring a first image at a given exposure time, wherein the first image comprises a fluorescence microscopy image of a biological material comprising a first biomarker; acquiring a second image at a different exposure time, wherein the second image comprises a fluorescence microscopy image of a biological material comprising a second biomarker; adjusting a pixel intensity of one or more pixels in the first image, based at least in part on the dark pixel intensity, for a second exposure time that is different from the given exposure time; and adjusting a pixel intensity of one or more pixels in the second image, based at least in part on the dark pixel intensity, for a second exposure time that is different from the given exposure time, wherein adjusting the pixel intensity at least in part uses the following equation: I t 2 = t 2 t 1 ⁢ I t 1 - ( t 2 t 1 - 1 ) ⁢ DarkpixelIntensity wherein t 1 is the given exposure time, t 2 is the second exposure time, I t1 is a signal intensity of a given pixel for exposure time t 1 , I t2 is an estimated signal intensity for exposure time t 2 and Darkpixellntensity is the dark pixel intensity for the imaging device.