Patent ID: 7749707

Claim:
A method for amplifying a polynucleotide, in which an amount existing in a sample (tester) is larger than the amount existing in another sample (driver), comprising the following steps: (1) forming a double-stranded polynucleotide consisting of a single-stranded polynucleotide derived from the tester and a single-stranded polynucleotide derived from the driver, which is a complementary strand with the single-stranded polynucleotide derived from the tester, by (i) performing hybridization after mixing the single-stranded polynucleotide derived from the tester and the single-stranded polynucleotide derived from the driver, which can be the complementary strand with the single-stranded polynucleotide derived from the tester, or (ii) performing hybridization after mixing the single-stranded polynucleotide derived from the tester and the double-stranded polynucleotide derived from the driver and subjecting to thermal denaturation; (2) removing the hybridized double-stranded polynucleotide by enzymatic treatment; (3) obtaining the double-stranded polynucleotide derived from the tester by amplifying the non-hybridized single-stranded polynucleotide derived from the tester and the complementary strand thereof; and (4) removing the single-stranded polynucleotide derived from the non-hybridized driver, which did not form a hybrid in step (2).