Patent ID: 8121365

Claim:
A method for identifying an optimal antibody titer for an immunoassay comprising: receiving pixelized images of a plurality of tissue sample sets, each tissue sample set comprising a plurality of different tissue samples prepared with respective titer dilution, different tissue sample sets having different respective titer dilutions; performing a quantitative analysis of the pixelized images of the plurality of tissue sample sets, resulting in a respective selectivity value indicative of a staining intensity of each of the different tissue samples of each of the plurality of tissue sample sets; determining from pixel intensities of the pixelized images of each of the different tissue samples of each of the plurality of tissue sample sets, a respective sensitivity value for each tissue sample set of the plurality of tissue sample sets; and identifying from the respective selectivity values and the respective sensitivity values of each of the plurality of tissue sample sets an optimal titer dilution, wherein the act of performing a quantitative analysis further comprising determining for each respective tissue sample of the plurality of tissue samples a localization ratio relating a first numeric value indicative of a staining intensity of a preferred sub-cellular compartment to a second numeric value indicative of a staining intensity of a non-preferred sub-cellular compartment, the preferred sub-cellular compartment being identified by a known marker localization, and further comprising averaging for each of the different tissue sample sets having different titer dilutions, a respective localization ratio determined from localization ratios of the different tissue samples of each of the plurality of tissue sample sets, the average localization ratio indicative of the localization ratio of a respective titer dilution.