Patent ID: 8802371

Claim:
A method for detecting the presence of STEC bacteria in a sample, said sample comprising nucleic acids, said method comprising: (a) providing a reaction mixture comprising a first primer, a second primer, and a probe for amplification and detection of at least a portion of: (i) SEQ ID NO: 688; wherein each of said first primer for the amplification of SEQ ID NO: 688, second primer for the amplification of SEQ ID NO: 688, and probe for the detection of SEQ ID NO: 688 comprises a 5′ end and a 3′ end; wherein said first primer for the amplification of SEQ ID NO: 688 comprises at least 15 contiguous nucleotides of SEQ ID NO: 145 or a sequence complementary thereto; and wherein said probe for the detection of SEQ ID NO: 688 comprises at least 15 contiguous nucleotides of SEQ ID NO: 160 or a sequence complementary thereto; and (ii) one or more nucleic acid sequences selected from the group consisting of: (A) SEQ ID NO: 686; wherein each of said first primer for the amplification of SEQ ID NO: 686, second primer for the amplification of SEQ ID NO: 686, and probe for the detection of SEQ ID NO: 686 comprises a 5′ end and a 3′ end; and wherein said first primer for the amplification of SEQ ID NO: 686 and probe for the detection of SEQ ID NO: 686 are selected from the group consisting of: (I) a first primer comprising at least 15 contiguous nucleotides of SEQ ID NO: 1 or a sequence complementary thereto and a probe comprising at least 15 contiguous nucleotides of SEQ ID NO: 16 or a sequence complementary thereto; (II) a first primer comprising at least 15 contiguous nucleotides of SEQ ID NO: 1 or a sequence complementary thereto and a probe comprising at least 15 contiguous nucleotides of SEQ ID NO: 49 or a sequence complementary thereto; and (III) a first primer comprising SEQ ID NO: 55 or a sequence complementary thereto and probe comprising SEQ ID NO: 56 or a sequence complementary thereto; and (B) SEQ ID NO: 687; wherein each of said first primer for the amplification of SEQ ID NO: 687, second primer for the amplification of SEQ ID NO: 687, and probe for the detection of SEQ ID NO: 687 comprises a 5′ end and a 3′ end; and wherein said first primer for the amplification of SEQ ID NO: 687 and probe for the detection of SEQ ID NO: 687 are selected from the group consisting of: (I) a first primer comprising at least 15 contiguous nucleotides of SEQ ID NO: 74 or a sequence complementary thereto and a probe comprising at least 15 contiguous nucleotides of SEQ ID NO: 89 or a sequence complementary thereto; (II) a first primer comprising SEQ ID NO: 120 or a sequence complementary thereto and a probe comprising SEQ ID NO: 121 or 122 or a sequence complementary thereto; and (III) a first primer comprising SEQ ID NO: 125 or a sequence complementary thereto and probe comprising SEQ ID NO: 126 or a sequence complementary thereto; and (b) performing PCR amplification of said nucleic acids of said sample using the reaction mixture of step (a); and (c) detecting the amplification of step (b).