Patent ID: 7090981

Claim:
A method for the preparation of CAV, comprising the steps of: a. inoculating a susceptible substrate with the CAV, b. propagating the virus, and c. harvesting CAV containing material, wherein the CAV is an isolated chicken anaemia virus (CAV), wherein the virus is neutralized by a reference sample comprising monoclonal antibody R2 secreted by a hybridoma cell line, having the identifying characteristics of a sample deposited at the ECACC under accession no. 00020304 and the virus is not a chicken anemia agent (CAA) virus attenuated by passaging wild type CAA virus in embryonated chicken eggs, wherein the virus induces lesions in chicken embryos, and wherein the antibody titre of the reference sample is at least 5 (log 2) per 50 μl when examined against 300–1000 TCID 5 per 50 μl of the CAV.