Patent ID: 7951572

Claim:
A method for detecting the activity of a tyrosine kinase, comprising the steps of: (1) measuring a mass of a peptide on a gold nanoparticle-based (AuNP-based) peptide chip by secondary ion mass spectrometry; (2) allowing the AuNP-based peptide chip to react with the tyrosine kinase to be measured for its activity; (3) measuring the mass of the peptide on the AuNP-based peptide chip after reacting with the tyrosine kinase by secondary ion mass spectrometry; and (4) measuring the change between the peptide mass measured in step (1) and the peptide mass measured in step (3) to determine the activity of the tyrosine kinase, wherein the AuNP-based peptide chip comprises: a glass support; a self-assembled monolayer constructed on the support using 11-mercaptoundecylamine (MUAM); a monolayer of gold nanoparticles (AuNPs) formed on the self-assembled monolayer by binding between the self-assembled monolayer and the AuNPs; and a peptide immobilized by the AuNPs; and the peptide on the AuNP-based peptide chip having a length expanded through a glycine linker, wherein the amino-terminal end of the peptide on the AuNP-based peptide chip is cysteine.