Patent ID: 7065236

Claim:
A method of determining an amount of at least one molecular specie comprising a sample, each molecular specie being indicated by a dye, said method comprising: determining an optical density of the sample from the image data, corresponding to an image of the sample captured by a color image acquisition device in a video-microscopy system, in each of a red, green, and blue channel and for a pixel in the image, so as to form a corresponding optical density matrix for the pixel; determining a relative absorption coefficient for the dye indicating each molecular specie, independently of the sample and in each of the red, green, and blue channels, so as to form a corresponding relative absorption coefficient matrix; and multiplying the optical density matrix by an inverse of the relative absorption coefficient matrix so as to form a resultant matrix for the pixel, the resultant matrix comprising the amount of each molecular specie, as indicated by the respective dye, for the pixel.