Patent ID: 8158419

Claim:
An isolated B lymphocyte hybridoma clone wherein the B lymphocyte hybridoma clone expresses an antibody comprising a heavy chain consisting of a human immunoglobulin heavy chain variable region and a mouse immunoglobulin heavy chain gamma constant region, wherein the isolated B lymphocyte hybridoma is derived from a fusion of: (a) a B lymphocyte from a transgenic mouse, wherein the genome of said transgenic mouse comprises inactivated murine heavy chain variable regions and an unrearranged human heavy chain immunoglobulin variable region operably linked to a mu constant region gene segment, wherein the unrearranged heavy chain variable region comprises multiple human V H gene segments, multiple human D gene segments, and multiple human J H gene segments, and wherein the mu constant region is selected from the group consisting of: (i) a human S-mu sequence and a human or mouse mu coding sequence; and (ii) a mouse S-mu sequence and a human or mouse mu coding sequence; and (b) a myeloma cell.