Patent ID: 8771738

Claim:
A method for applying a coating to an anatomical site on tissue of a living organism comprising: applying to the site a) a first aqueous solution comprising an oxidized dextran containing aldehyde groups, having a molecular weight of about 1,000 to about 1,000,000 Daltons, said oxidized dextran having an equivalent weight per aldehyde group of about 90 to about 1500 Daltons, said solution containing from about 5% to about 40% by weight of the oxidized dextran, followed by b) a second aqueous solution comprising a first polyethylene glycol comprising eight arms terminated by a primary amine group, wherein the first polyethylene glycol has a molecular weight of about 450 to about 200,000 Daltons, said solution containing from about 5% to about 70% by weight of the first polyethylene glycol, a second polyethylene glycol comprising four arms terminated by a primary amine group, wherein the second polyethylene glycol has a molecular weight of about 450 to about 200,000 Daltons, said solution containing from about 5% to about 70% by weight of the second polyethylene glycol, or the aqueous solution of (b) followed by the aqueous solution of (a), or premixing the aqueous solutions of (a) and (b) and applying the resulting mixture to the site before the resulting mixture completely cures.