Patent ID: 8901513

Claim:
A system for analyzing a sample comprising: an input light source; a double subtractive monochromator positioned to receive light from the input light source and to sequentially illuminate the sample with each of a plurality of wavelengths; a multi-channel fluorescence detector positioned to receive and detect multiple wavelengths of light emitted by the sample for each of the plurality of excitation wavelengths; an absorption detector positioned to receive and detect light passing through the sample; a reference detector; a beamsplitter positioned to direct a portion of light from the monochromator to the reference detector, wherein the computer adjusts at least one of the absorption and fluorescence measurements based on a signal from the reference detector and a computer in communication with the monochromator, the fluorescence detector, the reference detector, and the absorption detector, the computer controlling the monochromator to sequentially illuminate the sample with each of the plurality of wavelengths while measuring absorption and fluorescence of the sample based on signals received from the reference detector, the fluorescence detector, and the absorption detector, the computer configured to simultaneously trigger the reference detector, the fluorescence detector, and the absorption detector.