Patent ID: 7883864

Claim:
A method of decellularizing mammalian tissue comprising: a) immersing the mammalian tissue in a treatment solution comprising a detergent but lacking of a fixation chemical; b) irradiating said tissue with microwaves at a frequency of 2451 MHz for a net period of at least 1 hour while maintaining the temperature of the tissue in a range between 0° C. and 40° C.; c) washing the irradiated tissue to remove residual cells, leaving a washed tissue; whereby the tissue is decellularized even in deep interior portions if determined by: d) staining a specimen from the washed tissue from c) with hematoxylin-eosin stain and histologically evaluating the specimen by microscopic observation to determine any residual cellular nuclei; e) identifying the tissue as decellularized when the specimen is marked by absence of any residual cellular nuclei even in deep interior portions.