Patent ID: 8481267

Claim:
A method of generating a sequence embedded fingerprint pattern for identifying an organism in a sample comprising: (a) providing a sample comprising said organism, said organism comprising at least one nucleic acid; (b) combining said sample or the at least one nucleic acid therefrom with an amplification mix comprising at least one labeled oligonucleotide primer; (c) generating at least one labeled amplification product from the at least one nucleic acid of said organism using a nucleotide amplification technique employing said at least one labeled oligonucleotide primer; (d) combining said at least one labeled amplification product with products from a previously performed DNA sequencing reaction of a known organism to create a separation mix; and (e) separating said separation mix on the basis of oligonucleotide length in a fluorescent DNA sequencing instrument to generate a sequence embedded fingerprint pattern for said organism, wherein the sequence embedded fingerprint pattern is generated by perturbing sequence output at positions where fingerprint products are migrating with like-sized DNA sequence fragments.