Patent ID: 8217144

Claim:
An isolated T-cell receptor (TCR) having the property of binding to AAGIGILTV (SEQ ID NO:43) -HLA-A*0201 and comprising: a TCR α chain variable domain having: SEQ ID NO:1 or SEQ ID NO:1 except that one or more of amino acids 28D, 29R, 30G, 31S, 49M, 51I, 53S, 54N, 72Y, 94V, 95A, 96G, 97K, 98S, and 99T is mutated; and a TCR β chain variable domain having: SEQ ID NO:2; or SEQ ID NO:2 except that one or more of amino acids 45L, 51S, 52V, 53G, 54I, 76I, 100G, 101T, 102G, 103E, 104L, and 105F are mutated, said TCR having a K D for the said AAGIGILTV (SEQ ID NO:43)-HLA-A*0201 complex of less than or equal to 3 μM and/or an off-rate (k off ) of 1×10 −3 S − 1 or slower, wherein the α and β chain variable domains of the TCR are not simultaneously unmutated SEQ ID NO:1 and unmutated SEQ ID NO:2, respectively.