Patent ID: 8067554

Claim:
A process for purifying a glucagon-like peptide-1 (GLP-1) peptide from a mixture comprising said GLP-1 peptide and impurities, said process comprising: eluting said GLP-1 peptide and said impurities of said mixture from an anion exchange chromatography matrix using a solution comprising an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in the salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value is in the range where said GLP-1 peptide has a negative local or overall net charge different from the local or overall negative net charge of said impurities so as to remove said impurities and wherein the ratio of organic modifier to water in said solution on a weight percent basis is from 30:70 to 70:30.