Patent ID: 8455218

Claim:
A method comprising producing a soluble G-CSF protein in a Pseudomonad host cell, wherein at least 99% of the G-CSF protein comprises an N-terminal methionine, wherein the Pseudomonad host cell has a mutation in at least one gene encoding at least one protease, wherein the mutation results in the inactivation of the at least one protease, and wherein the at least one protease inactivated is PrtB; both PrtB and AprA; both the serine peptidase encoded by SEQ ID NO: 29 and the S41 protease encoded by SEQ ID NO: 30; an HtpX protease encoded by SEQ ID NO: 31; or both the serine peptidase encoded by SEQ ID NO: 29 and the Lon protease encoded by SEQ ID NO: 32.