Patent ID: 7049130

Claim:
A diagnostic test strip for providing an indication of at least one of the presence, the absence or the amount of an analyte of interest in a heterogeneous fluid sample, the test strip comprising: a first support layer comprising a sample receiving port for receiving the fluid sample; a second support layer comprising a reaction viewing port for displaying the indication; a plurality of operative layers between the first and second support layers, the first and second support layers securing the plurality of operative layers therebetween; the plurality of operative layers comprising no more than two operative layers, the no more than two operative layers comprising: a first operative layer in contact with the first support layer and in vertical alignment with the sample receiving port, the first operative layer comprising a material for receiving a fluid sample containing the analyte of interest; and a second operative layer having a first side at least partially in contact with the first operative layer, and an opposite side in contact with the second support layer and in vertical alignment with the reaction viewing port, the second operative layer comprising a reaction membrane for receiving the analyte of interest through the first operative layer, the second operative layer containing a reagent for reacting with the analyte of interest to produce a measurable change in color for display in the reaction viewing port.