Patent ID: 8338150

Claim:
A method for parallel delivery of two or more agents to a surface of one or more cell structures and into the cytoplasm of the one or more cell structures comprising: (a) providing at least two electrolyte-filled tubes together with a ground or counter electrode, (b) connecting the electrolyte-filled tubes to a voltage or current generator, (c) introducing at least two agents into the electrolyte solution contained in each electrolyte-filled tube, wherein each electrolyte-filled tube used comprises at least two agents, which are arranged in at least two discrete zones or bands whereby the at least two agents are sequentially delivered through the pores of the one or more cell structures, (d) placing the electrolyte-filled tubes at close distance to the surface of the one or more cell structures, (e) sequentially transporting the agents through the electrolyte-filled tubes to the surface of the one or more cell structures, and (f) focusing an electric field of a strength sufficient to obtain electroporation of the surface of the one or more cell structures on the one or more cell structures, resulting in formation of pores in the membrane surface of the one or more cell structures, and wherein the agents are sequentially transported through the pores formed in step (f) and into the cytoplasm of the one or more cell structures, wherein steps (a-f) are performed in consecutive order, with the exception that the order of steps (b), (c) and (d) may be altered, and that the order of steps (e) and (f) may be altered.