Patent ID: 8357496

Claim:
A method for the diagnosis or the screening of a subject or animal host to determine whether the subject or animal host has been infected with an arbovirus, comprising: (i) contacting a sample from the subject or animal with a solid support sensitized with an Ig binding protein which is directed against a specific class of Ig molecules of the subject or animal species under consideration and (ii) incubating the immunocomplex formed in (i) with a detector molecule comprising a hybrid protein comprising at least a dimer of an arboviral ED3 domain fused with an E. coli alkaline phosphatase (PhoA) having two mutations in its active site: D153G and D330N and comprising SEQ ID NO: 24, (iii) detecting said immunocomplex in said sample, (iv) correlating the detection of said immunocomplex to indicate that the subject or animal host has been infected with an arbovirus.