Patent ID: 7262037

Claim:
A method of producing D-(−)-3-hydroxybutyric acid comprising the steps of: (a) cloning genes phbA, phbB, ptb and buk into plasmids to construct new plasmids; (b) transforming the constructed plasmids into a bacterial strain to construct a recombinant strain; and (c) culturing the recombinant strain under conditions to produce D-(−)-3 hydroxybutryic acid, wherein the sequences of genes phbA and phbB are selected from the group consisting of GenBank Accession Numbers AF029714, AF078795, U47026, X93358, J04987, L01112, and L01113; the sequence of gene ptb is selected from the group consisting of L04468, AB035092, and AJ278958; and the sequence of gene buk is L04468 or AB035092.