Patent ID: 8338385

Claim:
A method of treating an ischemic limb disease in a subject comprising intramuscularly administering to the ischemic limb of said subject a hybrid Hepatocyte Growth Factor (HGF) construct comprising: (a) a first cDNA which has the same sequence as exons 1-4 of the human HGF gene wherein said exons 1-4 are arranged in sequential order without an intron therebetween, or degenerates thereof which do not alter the amino acid sequence encoded by said first cDNA, (b) a polynucleotide that has the same sequence as intron 4 of a HGF gene or a fragment thereof, and (c) a second cDNA which has the same sequence as exons 5-18 of the human HGF gene wherein said exons 5-18 are arranged in sequential order without an intron therebetween, or degenerates thereof which do not alter the amino acid sequence encoded by said second cDNA, wherein (b) is located between (a) and (c) and the HGF construct simultaneously encodes two heterotypes of human HGF; wherein said administration treats the ischemic limb disease in said subject.