Patent ID: 8278120

Claim:
A modified fluorescent protein of a GFP-like fluorescent protein from Chiridius poppei: Chiridius poppei yellowish-green fluorescent protein (CpYGFP), which is characterized in that it is any one of: a modified fluorescent protein comprising a full amino acid sequence wherein histidine (His) at the 52nd amino acid position is replaced with one amino acid selected from the aromatic amino acid group consisting of phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) in the full-length amino acid sequence (SEQ ID NO: 1) of the CpYGFP: MTTFKIESRI HGNLNGEKFE LVGGGVGEEG RLEIEMKTKD 60 KPLAFSPFLL SHCMGYGFYH FASFPKGTKN IYLHAATNGG YTNTRKEIYE DGGILEVNFR 120 YTYEFNKIIG DVECIGHGFP SQSPIFKDTI VKSCPTVDLM LPMSGNIIAS SYARAFQLKD 180 GSFYTAEVKN NIDFKNPIHE SFSKSGPMFT HRRVEETHTK ENLAMVEYQQ VFNSAPRDM 219 and exhibiting a red shifted fluorescence peak wavelength, when compared with the fluorescence peak wavelength of the CpYGFP; a modified fluorescent protein comprising a full amino acid sequence wherein the His at the 52nd amino acid position in the full-length amino acid sequence of the CpYGFP is replaced with one amino acid selected from the amino acid group consisting of alanine (Ala), valine (Val), isoleucine (Ile), leucine (Leu), glycine (Gly), cysteine (Cys), methionine (Met), serine (Ser) and threonine (Thr), and exhibiting a blue shifted fluorescence peak wavelength, when compared with the fluorescence peak wavelength of the CpYGFP; and a modified fluorescence protein comprising a full amino acid sequence wherein the His at the 52nd amino acid position in the full-length amino acid sequence of the CpYGFP is replaced with one amino acid selected from the amino acid group consisting of aspartic acid (Asp), asparagine (Asn), glutamic acid (Glu) and glutamine (Gln), and exhibiting a blue shifted fluorescence peak wavelength, when compared with the fluorescence peak wavelength of the CpYGFP.