Patent ID: 7807461

Claim:
A method for producing a homogenous cell population of mesenchymal stem cells, comprising: culturing human adipose tissue-derived pellets in a medium containing N-acetyl-L-cysteine (NAC); removing unattached cells from the culture; culturing the remaining attached cells in a medium containing NAC to produce a homogenous population of human adipose tissue-derived mesenchymal stem cells wherein the cells are characterized by: (a) showing positive immunological responses to all of CD73, CD90, CD29, CD44 and CD 105, and negative immunological responses to all of CD33, CD34, CD45, CD4, CD31, CD62p, CD14 and HLA-DR; (b) growing attached to a plastic material, showing spindle-shaped morphological features, and forming spheres in a medium containing CORM-2 so as to be able to be maintained in an undifferentiated state; and (c) having the ability to differentiate into cells selected from the group consisting of mesoderm-derived cells, nerve cells, and pancreatic cells.