Patent ID: 8658607

Claim:
A method of inducing an immune response in a subject to an antigen, the method comprising: administering a composition comprising an isolated single-stranded RNA (ssRNA) G,U-rich oligoribonucleotide 5-40 nucleotides long having at least 80 percent G and U to a subject via a route of administration selected from the group consisting of mucosal, oral, intranasal, sublingual, ocular, vaginal, rectal, buccal, and by inhalation, wherein the G,U-rich oligoribonucleotide is free of CpG dinucleotide, wherein the administration requires cationic lipids, wherein the ssRNA is a ligand of Toll-like receptor 7 (TLR7) or TLR8, wherein the composition further comprises an antigen, and wherein the composition comprises an effective amount of the ssRNA G,U-rich oligoribonucleotide to induce an immune response to the antigen.