Patent ID: 8911683

Claim:
A microfluidic device comprising: a substrate, a cover, at least one chamber formed between the substrate and the cover, said chamber comprising two opposite surfaces, one on the substrate and one on the cover respectively, wherein the surface of the substrate comprises at least one hydrophilic area and at least one adjoining hydrophobic area, the surface of the cover comprises at least one hydrophilic area and at least one adjoining hydrophobic area, and the at least one hydrophilic area on each said opposite surface of the chamber is confined within or surrounded by said adjoining hydrophobic area, and is in fluid communication with at least one lateral hydrophilic area configured for guiding liquids to the at least one chamber, and wherein said device is configured such that at least two liquids enter the at least one chamber, each wetting said hydrophilic area on one respective surface of the chamber without touching the opposite respective surface until the liquid confined to the hydrophilic area on each respective surface of the chamber coalesce and mix with each other as volume of the two liquids increases within the chamber.