Patent ID: 8361292

Claim:
A method for analyzing hemoglobin by a capillary electrophoresis method, comprising: a sample-providing step of providing a sample containing hemoglobin; a capillary tube-providing step of providing a capillary tube containing a buffer solution; and an electrophoresis step of carrying out electrophoresis of the sample, by introducing the sample into the buffer solution of the capillary tube, and applying a voltage across both ends of the capillary tube; wherein the hemoglobin is HbA1c and is analyzed following at least one of modes (A) and (B) below: (A) the electrophoresis is carried out with the capillary tube filled with the buffer solution to which a surfactant (a) has been added in the range of 0.005 to 0.05 wt % by causing the buffer solution to pass through the capillary tube in the capillary tube-providing step, by injecting the sample into the capillary tube with the capillary tube being filled with the buffer solution, the surfactant (a) being a non-ionic surfactant having an alkyl group as a hydrophobic portion and a sugar as a hydrophilic portion; (B) the electrophoresis is carried out with a surfactant (b) added to the sample in the range of 0.001 to 0.1 wt % in the sample-providing step and the capillary tube filled with the buffer solution by causing the buffer solution to pass through the capillary tube in the capillary tube-providing step, by injecting the sample into the capillary tube with the capillary tube being filled with the buffer solution, the surfactant (b) being a betaine-type amphoteric surfactant; and wherein HbA0 and HbS are separated.