Patent ID: 8501204

Claim:
A method for producing a vesicle, which comprises the following steps (1) to (3): (1) a step of producing a W/O emulsion from an aqueous solution containing a substance to be entrapped in a vesicle in a dissolved or suspended state, and an organic solvent phase containing a vesicle-constituting lipid having emulsification capacity, which can constitute the vesicle, and a stabilizer lipid selected from the group consisting of sterols, charged lipids, long chain fatty acids, and mixtures thereof; (2) a step of producing a W/O/W emulsion from the W/O emulsion and an external water phase solution containing a protein emulsifier comprising casein sodium as a water-soluble emulsifier, which does not destroy a vesicle lipid membrane; and (3) a step of removing the organic solvent phase from the W/O/W emulsion, so as to form a vesicle.