Patent ID: 7425614

Claim:
An isolated complex comprising an NR2B polypeptide and a RasGRF1 polypeptide, wherein said NR2B polypeptide consists essentially of: (a) an amino acid sequence having at least 95% identity to the amino acid sequence comprising residues 886-1310 set forth in SEQ ID NO: 2 or 6 and which is less than 500 amino acids in length; (b) the amino acid sequence comprising residues 886-1310 set forth in SEQ ID NO: 2 or 6 with 1 to 10 conservative amino acid substitutions and which is less than 500 amino acids in length; or (c) the amino acid sequence comprising residues 886-1310 set forth in SEQ ID NO:2 or 6 and which is less than 500 amino acids in length; wherein the polypeptides of (a), (b), or (c) are capable of binding to a RasGRF1 polypeptide; and wherein said RasGRF1 polypeptide consists essentially of: (a) an amino acid sequence having at least 95% identity to the amino acid sequence comprising residues 714-913 set forth in SEQ ID NO: 4 or comprising residues 732-942 set forth in SEQ ID NO: 8 and which is less than 500 amino acids in length; (b) the amino acid sequence comprising residues 714-913 set forth in SEQ ID NO: 4 or comprising residues 732-942 set forth in SEQ ID NO: 8 with 1 to 10 conservative amino acid substitutions and which is less than 500 amino acids in length; or (c) the amino acid sequence comprising residues 714-913 set forth in SEQ ID NO: 4 or comprising residues 732-942 set forth in SEQ ID NO: 8 and which is less than 500 amino acids in length; wherein the polypeptides of(a), (b), or (c) are capable of binding to an NR2B polypeptide.