Patent ID: 8592212

Claim:
A method of germline transforming and culturing a soybean plant cell using Agrobacterium mediation, the method comprising: (a) initiating the germination of a soybean seed; (b) isolating the embryonic axis including the embryonic meristem from the soybean seed to prepare an explant; (c) wounding the explant; (d) exposing the explant to a disarmed Agrobacterium vector comprising a heterologous genetic construct comprising a selectable marker gene wherein the heterologous genetic construct is transferred into at least one cell in the explant; and (e) culturing the explant in the presence of a selection agent in a manner allowing identification of soybean cells of the explant to which the heterologous genetic construct has been transferred and in the presence of a shoot inducing agent, wherein the selection agent is glyphosate and the shoot inducing agent is only glyphosate.