Patent ID: 7989212

Claim:
A method for detecting the presence of schizandrin B in the blood plasma of an animal after administering the botanical extract composition Strengthening Body Resistance and Dissipating Blood Stasis Botanical to said animal, comprising the steps of: a. collecting the blood plasma from said animal 0.5 hours after administration of Strengthening Body Resistance and Dissipating Blood Stasis Botanical to said animal; b. extracting said blood plasma with ethyl acetate in a blood plasma to ethyl acetate ratio of 1:4 (v/v) for 3 minutes; c. centrifugating the product resulting from step b at 9600 rpm for 10 minutes; d. collecting the supernatant after said centrifugation and drying said supernatant at 25-30° C. to produce a dried supernatant; e. redissolving said dried supernatant in a mixture of water-acetonitrile-formic acid at a ratio of water:acetonitrile:formic acid equals to 95:5:0.1 (v/v/v) to form a blood plasma solution; f. applying said blood plasma solution to a liquid chromatography column having a size of 2.1×100 mm, wherein the chromatography is performed having conditions including a mobile phase A and a mobile phase B, said mobile phase A is the mixture of water-acetonitrile-formic acid in step e and said mobile phase B is a mixture of acetonitrile-formic acid in a ratio of acetonitrile:formic acid equals to 100:1 (v/v); and g. detecting the presence of schizandrin B with mass scan at a range of m/z 150-1200, using an electrospray ionization (ESI) with a positive ion mode.