Patent ID: 8124064

Claim:
An in-vitro deposition evaluation method comprising the steps of: a. providing a microplate comprising a base comprising a surface comprising a plurality of wells defined in said surface; b. providing at least one body in each of said plurality of wells; each body capable of movement within said well; c. providing a sample comprising a personal care composition; d. depositing a volume of said sample into each of said plurality of wells sufficient to submerge said at least one body; e. providing a skin mimic comprising a treated test region; wherein the skin mimic comprises: (1) a substrate comprising a polymer; (2) a coating layer comprising a plasma-deposited coating material selected from the group consisting of allyl amine, allyl alcohol, 1,1,1-trimethyl-1-pentene, 2,4,4-trimethyl-1-pentene, perfluoromethylcyclohexane monomer, and combinations thereof, wherein: i. said coating layer is stably affixed to said substrate to form a stable, coated surface, wherein said coated surface has a texture that mimics the topography of mammalian keratinous tissue; and ii. said coated surface demonstrates at least one physical property representative of mammalian keratinous tissue, selected from the group consisting of a total surface energy of from about 15 mJ/m 2 to about 50 mJ/m 2 , a dispersive component of the surface energy of from about 15 mJ/m 2 to about 50 mJ/m 2 , a polar component of the total surface energy of from about 1 mJ/m 2 to about 14 mJ/m 2 , a zeta-potential at a pH of about 5.0 of from about −40 mV to about 30 mV, and combinations thereof; and f. contacting said treated test region to said surface of said microplate such that said treated test region is exposed to said sample and said body within each of said plurality of wells; g. causing said at least one body to move wherein a portion of said sample is transferred to said treated test region of said skin mimic; and h. quantifying an amount of said sample that is transferred to said treated test region of said skin mimic.