Patent ID: 8741280

Claim:
A Bacillus composition comprising 10 5 to 10 12 CFU/g isolated Bacillus spore cells, wherein (i) the Bacillus spore cells reach a vegetative cell growth point of 0.4 OD 630 after less than 18 hours and 19 hours in the presence of a 4 mM bile salt medium and a 6 mM bile salt medium, respectively, wherein each of the 4 mM bile salt medium and the 6 mM bile salt medium comprise non-selective veal infusion broth medium supplemented with a bile salt mixture comprising 60% taurodeoxycholate, 30% glycodeoxycholate, and 10% deoxycholate, and (ii) the Bacillus vegetative cells produce at least one essential amino acid in an amount higher than Bacillus cell DSM 19467 after incubating for 2 days at 37° C. in a minimal salts growth medium.