Patent ID: 8278062

Claim:
A method of producing a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxy acid ester, wherein the method comprises contacting an acyl donor, an acyl acceptor and 5-98% water with a lipid acyltransferase enzyme capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, and glycerol, whereby the carbohydrate ester, the protein ester, the protein subunit ester or the hydroxy acid ester is produced; and wherein the lipid acyltransferase when tested using the Transferase Assay in Buffered Substrate has at least 5% acyltransferase activity (relative acyltransferase activity) wherein the Transferase Assay in Buffered Substrate comprises: (a) heating to 35° C. a substrate solution comprising phosphatidylcholine, cholesterol, water and HEPES buffer, wherein the substrate solution comprises approximately 95% water and has pH 7.0; (b) adding an enzyme to the substrate solution; and (c) determining acyltransferase activity of the enzyme based upon cholesterol ester and fatty acids formed.