Patent ID: 8173399

Claim:
A method for producing lacto-N-biose I, wherein the method comprises causing: (i) a combination of a carbohydrate raw material with an enzyme that catalyzes phosphorolysis of the carbohydrate raw material to give α-glucose-1-phosphate; and (ii) (a) an enzyme that converts α-glucose-1-phosphate and UDP-galactose to UDP-glucose and α-galactose-1-phosphate, respectively, with cofactor(s) and substrate(s) of said enzyme of (a); and/or (b) a combination of an enzyme that converts α-glucose-1-phosphate to UDP-glucose and an enzyme that converts UDP-galactose to α-galactose-1-phosphate with cofactor(s) and substrate(s) of said enzymes of (b); and/or (c) a combination of an enzyme having both activity of glucose-1-phosphate uridylyltransferase and activity of galactose-1-phosphate uridylyltransferase and cofactor(s) and substrate(s) of said enzyme of (c); to act in the presence of N-acetylglucosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211), 1,3-β-galactosyl-N-acetylhexosamine phosphorylase, and UDP-glucose-4-epimerase (EC 5.1.3.2), wherein lacto-N-biose I is obtained.