Patent ID: 6875590

Claim:
An isolated polynucleotide comprising a first nucleotide sequence comprising the coding region for a first leader peptide having the amino acid sequence M-X n -(K/R)-(K/R)-J m -P-X p -Z-X-Z, wherein M is methionine, K is lysine, R is arginine, (K/R) represents either lysine or arginine, P is proline, each X is independently any genetically encoded amino acid, each J is independently an amino acid selected from the group consisting of alanine, leucine, valine, phenylalanine, threonine, isoleucine, seine, glutamine, asparagine, methionine, and tyrosine, each Z is independently an amino acid selected from the group consisting of alanine, seine, glycine, valine and threonine, n is an integer from 1 to 2, p is an integer from 0 to 2, and m is an integer from 7 to 16; and a second nucleotide sequence comprising a first ribosome binding site, wherein said second nucleotide sequence is 5′ of said first nucleotide sequence, wherein said first ribosome binding site is operatively joined to said coding region for said first leader peptide, and wherein, when said polynucleotide is RNA or is transcribed into RNA, said first ribosome binding site is accessible.