Patent ID: 8263379

Claim:
A modified Family 6 glycosidase comprising an amino acid substitution at position 367 selected from the group consisting of W367G, W367A, W367C, W367N, W367R, W367S, W367T, and W367V, said position determined from alignment of a parental Family 6 glycosidase amino acid sequence with a Trichoderma reesei Cel6A amino acid sequence as defined in SEQ ID NO: 1, wherein amino acids 83-447 (TrCel6A numbering) of said modified Family 6 glycosidase are from about 90% to about 99.9% identical to amino acids 83-447 of SEQ ID NO: 1 and wherein said modified Family 6 glycosidase exhibits at least a 1.2-fold increase in hydrolysis activity of beta 1-3, 1-4 -linked polysaccharides and at least a three-fold decrease in hydrolysis activity of beta 1-4 linked polysaccharides, compared with said parental Family 6 glycosidase from which said modified Family 6 glycosidase is derived.