Patent ID: 8101347

Claim:
A method of diagnosing Pseudomonas aeruginosa infection comprising: (a) exposing a patient's sample to a nucleotide probe, wherein the probe hybridizes specifically to nucleotides between residues 626-1510 of SEQ ID NO:8 or nucleotides encoding amino acid residues 144-257 of SEQ ID NO: 7, and wherein the probe comprises a single nucleotide sequence consisting of at least forty contiguous nucleotides between residues 626-1510 of SEQ ID NO:8; (b) detecting the hybridization via PCR or RT-PCR with two primers, wherein each primer consists of a single nucleotide sequence consisting of at least 25 continuous nucleotides between residues 626-1510 of SEQ ID NO:8; and (c) correlating Pseudomonas aeruginosa infection with the presence of the hybridized probe.