Patent ID: 8420789

Claim:
A method for removing DNA contaminants from a genetically produced antibody-containing sample, which consists of the steps of: 1) applying the sample to a protein-A or protein-G affinity column, which binds the antibody, 2) washing the column to elute non-bound components of the sample, 3) eluting the bound antibody with an aqueous solution of pH of 2.0 to 3.9 and an ionic concentration of 50 mM or less, thereby forming an acidic aqueous solution of the antibody having an ionic concentration of 50 mM or less and a pH of 2.0 to 3.9, 4) forming particles containing the DNA contaminants by a step consisting of adjusting the pH of the eluate solution to a pH of from 4.0 to 7.5, and 5) removing the particles of step 4) wherein the sample is a culture medium from an antibody-producing cell culture.