Patent ID: 7193077

Claim:
A method of forming an intemucleotide bond, the method comprising contacting a nucleoside moiety having a reactive site hydroxyl with a precursor having an exocyclic amine triaryl methyl protecting group under conditions and for a time sufficient to result in internucleotide bond formation, said precursor having the structure (I), Rag-Sugar-Base-Tram (I) wherein: Rag is a reactive group capable of reacting with the reactive site hydroxyl, Sugar is a sugar group having a hydroxyl protecting group, Base is a heterocyclic base having an exocyclic amine group Tram is the exocyclic amine triaryl methyl protecting group wherein the reactive group has the structure (IV): wherein: The broken line indicates the bond to the sugar group, X is selected from a halogen or a secondary amino group, and Y is selected from hydrido, hydrocarbyl, substituted hydrocarbyl, benzyl, substituted benzyl, electron-withdrawing β-substituted aliphatic, electron-withdrawing substituted phenyl, or electron-withdrawing substituted phenylethyl.