Patent ID: 8362227

Claim:
An article of manufacture, comprising: a pair of tcdC primers, wherein said pair of tcdC primers comprises a first tcdC primer and a second tcdC primer, wherein said first tcdC primer comprises the sequence 5′-ACC TCA TCA CCA TCT TCA ATA AC-3′ (SEQ ID NO:1), and wherein said second tcdC primer comprises the sequence 5′-TCA AAA TGA AAG ACG ACG AAA -3′ (SEQ ID NO:2); a pair of tcdC probes, wherein said pair of tcdC probes comprises a first tcdC probe and a second tcdC probe, wherein said first tcdC probe comprises the sequence 5′-TTC AGC CTT TTT AGC TTC TTC AGC -3′ (SEQ ID NO:3), and wherein said second tcdC probe comprises the sequence 5′-TTA CGT TGA TTT TCA GCT TCA ATA GC -3′ (SEQ ID NO:4); and a donor fluorescent moiety and a corresponding acceptor fluorescent moiety.