Patent ID: 8653006

Claim:
A method of determining a stage in the progression of an esophageal adenocarcinoma in a subject, comprising the steps of: measuring the concentrations of the at least two components of a panel of a plurality of metabolic biomarkers consisting of lysine, lactate, leucine, valine, methionine, tyrosine, myristic acid, margaric acid, linolenic acid, pyroglutamic acid, proline, histidine, alanine and glutamate in a test sample of a biofluid from the subject to be tested for a stage in the progression of an esophageal adenocarcinoma, wherein the components of the panel have been selected from a population of metabolic biomarkers that have been shown to significantly distinguish a first stage from a second stage in the progression of an esophageal adenocarcinoma; and wherein a statistical model has been constructed using the combined measured concentration differences of each component in the panel of a plurality of biomarkers in a first sample from a subject in the first stage and the measured concentration of each component of the panel of a plurality of biomarkers in a second sample from a subject in the second stage, wherein the statistical model distinguishes the first sample from the second sample; using the statistical model to determine the relationship of the concentrations of the components of the panel of metabolic biomarkers measured in the test sample to the concentrations of the components of the panel of metabolic biomarkers that are characteristic of the first stage and characteristic of the second stage; thereby determining the stage in the progression of the esophageal adenocarcinoma in a subject.