Patent ID: 8067192

Claim:
A method for detecting the presence of a botulinum toxin in a sample comprising: a) exposing the sample putatively containing a botulinum toxin to (i) an enrichment matrix comprising a botulinum-specific antibody that captures botulinum toxin in the sample and (ii) a substrate composition comprising a toxin substrate that is capable of eliciting a detectable fluorogenic signal when modified by the botulinum toxin, the substrate selected from (SEQ ID NO: 12) Lys[5-Fam]IleAspGluAlaAsnGlnArgAlaThrLys[DABCYL]X, wherein X is norleucine; (SEQ ID NO: 13) 5-Fam- Lys[5-Fam]IleAspGluAlaAsnGlnArgAlaThrLys[DABCYL]X, wherein X is norleucine; or (SEQ ID NO: 14) 5-Fam-LysIleAspGluAlaAsnGlnArgAlaThrLys[DABCYL]Nle, wherein the exposure occurs under conditions permitting binding of the botulinum toxin to the antibody and modification of the substrate by the botulinum toxin; and b) detecting the presence of the botulinum toxin by measuring a change in fluorescence in the sample.