Patent ID: 7355710

Claim:
An optical system, for exciting and measuring fluorescence on or in samples treated using fluorescent pigments, including: at least one first laser which emits bundled light of a first wavelength to excite first fluorescent pigments, so that these first fluorescent pigments emit light of a second wavelength; a deflection element that is implemented to separate an excitation beam of the first wavelength from a fluorescent light of the second wavelength; a mirror, for deflecting the bundled light of the first wavelength, which comes out of the first laser and is incident on the mirror, in the direction of a sample or for deflecting light of the second wavelength, originating from the sample and being incident on the mirror, in the direction to a detector; an optic, for producing a first focal point on or in the sample for the bundled light of the first wavelength, which is incident from the first laser and is deflected by the mirror; a unit, which includes the mirror and optic, the mirror and optic being positioned fixed in relation to one another in the unit and the unit being positioned so it is linearly movable back and forth and being mechanically connected to an oscillating linear drive, the optic additionally being implemented as a collimator for the light of the second wavelength emitted by the first fluorescent pigment on or in the sample, and the mirror additionally being implemented for deflecting the collimated light diametrically opposite to the direction of incidence of the bundled light of the first wavelength; a table for receiving sample holders for samples treated using at least one first fluorescent pigment; an optical arrangement for imaging a second focal point using the light of the second wavelength emitted by the first fluorescent pigment, collimated by the optic, and deflected by the mirror; an aperture plate, positioned in the second focal point; a first spectral filter for selecting a component of the light of the second wavelength passing through the aperture plate; and a first detector for measuring the intensity of the light of the second wavelength which passes through the aperture plate and is selected by the first spectral filter, wherein a focusing beam of the light, which is emitted by the laser and is deflected by the deflection element into the direction of the mirror, is arranged to extend off-center and in a distance A to an optical axis of the optical system.