Patent ID: 8501446

Claim:
A method of inhibiting JAK-3, ROCK, or Aurora kinase activity in a biological sample comprising the step of contacting said biological sample with a compound of formula (I): or pharmaceutically acceptable salts thereof, wherein: R 1 is —H; R 2 , R 3 , and R 4 are each independently halogen, —CN, —NO 2 , or V—R′; X 1 , X 2 and X 3 are each independently N, or CH, wherein the hydrogen atom of CH is optionally replaced by R 5 ; x is 1, 2, 3, or 4; each occurrence of R 5 is independently halogen, CN, NO 2 , or U—R′, wherein at least one R 5 is other than H; V, and U are each independently a bond or an optionally substituted C 1 -C 6 alkylidene chain, wherein up to two methylene units of the chain are optionally and independently replaced by —NR′—, —S—, —O—, —CS—, —CO 2 —, —OCO—, —CO—, —COCO—, —CONR′—, —NR′CO—, —NR′CO 2 —, —SO 2 NR′—, —NR′SO 2 —, —CONR′NR′—, —NR′CONR′—, —OCONR′—, —NR′NR′—, —NR′SO 2 NR′—, —SO—, —SO 2 —, —PO—, —PO 2 —, or —POR′—; and each occurrence of R′ is independently hydrogen or an optionally substituted group selected from a C 1 -C 6 aliphatic group, a 3-8-membered saturated, partially unsaturated, or fully unsaturated monocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or an 8-12 membered saturated, partially unsaturated, or fully unsaturated bicyclic ring system having 0-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two occurrences of R′, are taken together with the atom(s) to which they are bound to form an optionally substituted 3-12 membered saturated, partially unsaturated, or fully unsaturated monocyclic or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, provided that: a) if R 1 , R 2 , and R 3 are simultaneously H and R 4 is selected from H or Me, x is 1, X 1 and X 3 are CH, then X 2 is not C—R 5 , where R 5 is OMe, NO 2 , or fluoro; b) if R 1 , R 2 , R 3 and R 4 are simultaneously H, x is 1, R 5 is —SMe, —NH 2 or an optionally substituted NH-piperidine, and X 1 and X 2 are N, then X 3 is not CH; c) if R 1 , R 2 , and R 4 are simultaneously H and (i) X 2 and X 3 are CH or CR 5 or (ii) any one of X 1 , X 2 or X 3 are N, then R 3 is not phenyl or phenyl substituted with O-phenyl or N(Me) 2 .