Patent ID: 8546141

Claim:
A method for producing blood cells, the method comprising: establishing clonal populations of human-derived induced pluripotent stem (iPS) cells; plating the iPS cells onto OP9 cells or 10T1/2 cells, and then culturing the iPS cells under conditions suitable for inducing differentiation of hematopoietic progenitor cells, thereby forming sac-like structures containing said hematopoietic progenitor cells; selecting human-derived iPS cell clones forming 10 or more sac-like structures per 1×10 5 cells; plating the selected iPS cell clones onto OP9 cells or 10T1/2 cells, and then culturing under conditions suitable for inducing differentiation of hematopoietic progenitor cells, thereby forming sac-like structures containing said hematopoietic progenitor cells; wherein each of the sac-like structures takes a follicular structure and has septa composed of UEA-1 lectin positive cells; separating cells forming the septum of the sac-like structures from the hematopoietic progenitor cells, both of which are produced by the iPS cell clones;