Patent ID: 8748133

Claim:
A method for detecting Listeria in a sample, the method comprising: performing a nucleic acid amplification assay using a set of oligonucleotides comprising two or more T7 provider oligonucleotides and one or more primer oligonucleotides, wherein the two or more T7 provider oligonucleotides and the one or more primer oligonucleotides are configured such that nucleic acids from L. monocytogenes, L. innocua, L. grayi, L. ivanovii, L. welshimeri, L. murrayi , and L. seeligeri are amplifiable under nucleic acid amplification assay conditions without substantial amplification of nucleic acid from Brochothrix thermosphacta or Erysipelothrix rhusiopathiae, wherein at least one of the T7 provider oligonucleotides targets a sequence in a region of Listeria nucleic acid corresponding to nucleotide positions of about 49-68 of SEQ ID NO: 103, and wherein at least one of the one or more primer oligonucleotides targets a sequence in a region of Listeria nucleic acid corresponding to nucleotide positions of about 90-156 of SEQ ID NO: 103; and detecting presence or absence of an amplification product, wherein presence of the amplification product indicates the presence of Listeria in the sample.