Patent ID: 7057031

Claim:
A method of preparing a marker-photocleavable linker-nucleotide conjugate comprising: a) providing i) a photocleavable linker comprising a protective group, ii) an amino-nucleotide analog, and iii) an activated marker molecule selected from the group consisting of BODIPY-FL-SF and Cy5-NHS; b) operably linking said photocleavable linker to said amino-nucleotide analog to produce a photocleavable linker-nucleotide conjugate; c) removing said protective group from said photocleavable linker-nucleotide conjugate under conditions such that an activated photocleavable linker-nucleotide conjugate is created, wherein said activated photocleavable linker-nucleotide conjugate has an exposed reactive site on the linker portion of the conjugate; and d) contacting said activated marker molecule with said activated photocleavable linker-nucleotide conjugate under conditions such that a marker-photocleavable linker-nucleotide conjugate is produced.