Patent ID: 7910298

Claim:
A method for a screening detection of genetic modifications in a genome comprising: selecting as a target nucleotide site of a cell genome, a target sequence portion of the cell genome to be genetically modified; providing a vector comprising an exogenous nucleotide sequence flanked by two recombination arms corresponding to two target sequences flanking the target sequence portion of the cell genome; wherein, at least one of the two recombination arms comprises in its sequence, a modified nucleotide, said modified nucleotide being different from a corresponding nucleotide existing in the corresponding target nucleotide sequence of the cell genome; transforming cells by the vector and obtaining transformed cells wherein an homologous recombination between the arms of the vector and the corresponding target nucleotide sequences of the genome of the cell occurs; selecting and cloning the transformed cells into clones, wherein said clones are not human beings; extracting the genomic DNA of the clones; amplifying with primers the recombination arm sequence comprising the modified nucleotide or its complementary strand sequence, into amplified sequences; detecting and selecting by a quantitative sequencing of the amplified sequences, clones wherein only the target sequence portion of the cell genome has been replaced by the exogenous nucleotide sequence; and recovering the detected and selected clones.