Patent ID: 8551768

Claim:
A non-naturally occurring binary oligonucleotide peroxidase probe (BOPP) for detecting a single stranded oligonucleotide analyte comprising two oligonucleotide strands, wherein 1. a first oligonucleotide strand comprises: a. at its 5′-terminus an oligonucleotide hemin-binding arm that is flanked by a first oligonucleotide analyte binding arm, and b. at its 3′-terminus, the first oligonucleotide analyte binding arm that is complementary to and selectively hybridizes with a first unique region of the oligonucleotide analyte; 2. a second oligonucleotide strand comprises: a. at its 3′-terminus an oligonucleotide hemin-binding arm that is flanked by a second oligonucleotide analyte binding arm, and b. at its 5′-terminus, the second oligonucleotide analyte binding arm that is complementary to and selectively hybridizes with a second unique region of the oligonucleotide analyte that is adjacent to the first unique region; and 3. a.) one of either the first or the second oligonucleotide strands comprises a non-nucleotide linker while the other strand comprises a nucleotide linker that is at least two nucleotides long between the oligonucleotide hemin-binding arm and the oligonucleotide analyte binding arm, or b.) both the first and the second oligonucleotide strands comprise a non-nucleotide linker between the oligonucleotide hemin-binding arm and the oligonucleotide analyte binding arm.