Patent ID: 8058017

Claim:
A method for purifying human factor VIII, a fragment of human factor VIII, or a factor VIII-like peptide wherein said fragment or factor VIII-like peptide retains the procoagulant properties of human factor VIII, the method comprising: (a) immobilizing a polypeptide on a solid support, wherein the polypeptide comprises the amino acid sequence X 1 â€”X 2 -Cys-X 3 â€”X 4 -Trp-X 5 â€”X 6 -Pro-Cys-X 7 â€”X 8 (SEQ ID NO:2), wherein: X 1 is Arg or His; X 2 is Gly, Ala, Arg, Leu or Pro; X 3 is Gly or Phe; X 4 is Ala or Ser; X 5 is Phe or Leu; X 6 is Arg, Asn or His; X 7 is Ala, Asp, His, Leu, Phe, Pro or Tyr; and X 8 is Ala, Arg, Asn, Asp or His, such that the polypeptide binds factor VIII and such that a terminal amino acid residue comprises a chemical modification; (b) contacting a solution containing said human factor VIII, fragment of human factor VIII, or factor VIII-like peptide with the immobilized polypeptide on a solid support, and (c) purifying said human factor VIII, fragment of human factor VIII, or factor VIII-like peptide by separating the solution from said solid support.