Patent ID: 7642046

Claim:
A method for monitoring binding of a protein to the intracellular domain of a receptor in a cell, comprising: a) inducing signalosome formation in cells, wherein the signalosome is a multi-protein complex comprising a receptor, one or more adaptor proteins and one or more signaling proteins, and the signalosome forms in the intact natural cellular environment within the cell; b) lysing the cells; c) incubating the cell lysate on a solid phase coated with a first antibody capable of capturing the receptor within the signalosome; d) separating the solid phase from the cell lysate; and e) detecting and measuring the amount of a protein component of the signalosome using a second antibody capable of detecting the protein to monitor the binding of the protein to the intracellular domain of the receptor as part of the intact signalosome, wherein the signalosome remains intact and bound to the solid phase of c) during detecting and measuring.