Patent ID: 7919327

Claim:
A method of measuring a liquid volume of a sample solution in a vessel, wherein the vessel includes a bottom and known dimensions, the method comprising the steps of: a. adding to the vessel a diluent including a known concentration of a diluent chromophore which absorbs light at a second wavelength; b. measuring the absorbance of the diluent chromophore at the second wavelength; c. adding a volume of the sample solution to the vessel, wherein the sample solution includes a known concentration of a sample solution chromophore which absorbs light at a first wavelength, and wherein the sample solution does not include the diluent chromophore; d. mixing the diluent and the sample solution in the vessel to produce a mixture of the sample solution and the diluent; e. measuring the absorbance of the mixture of the sample solution and the diluent at the first wavelength and at the second wavelength; and f. calculating the volume of the sample solution added to the vessel based on the measured absorbances at the first wavelength and the second wavelength.