Patent ID: 7989163

Claim:
A method for detecting a target biological-related substance, comprising the steps of: (a) labeling a biological-related substance using a fluorophore, (b) capturing the biological-related substance in a portion for light detection, (c) irradiating an excitation light for exciting the fluorophore onto the portion for light detection, (d) detecting a light emitted by the portion for light detection after dividing the light into a plurality of wavelength zones, wherein one of the plurality of wavelength zones is substantially the same as that of a wavelength component of the excitation light, (e) simultaneously monitoring an intensity of the component of the excitation light wavelength and the intensity of the light emitted by the fluorophore, (f) comparing the light intensity of the wavelength zone that is substantially the same as that of the component of the excitation light with a predetermined intensity range obtained from a normal sample, (g) quantifying the target biological-related substance, and, (h) in the case where the light intensity of the wavelength zone that is substantially the same as that of the component of the excitation light exceeds the predetermined intensity range, judging that the light intensity emitted by the fluorophore in the portion for light detection is not appropriate, (i) in the case where the light intensity of the wavelength zone that is substantially the same as that of the component of the excitation light does not exceed the predetermined intensity range, correcting the light intensity emitted by the fluorophore using the light intensity of the wavelength zone that is substantially the same as that of the component of the excitation light.