Patent ID: 8101733

Claim:
A method of analyzing an enoxaparin sample, comprising: providing an enoxaparin sample comprising a plurality of polysaccharide molecules; detecting a Δ4,5 double bond associated with a non-reducing end of at least a portion of the polysaccharide molecules in the enoxaparin sample by UV absorbance, to determine the total number of chains in the sample; detecting at least a portion of the polysaccharide molecules in the enoxaparin sample having a reducing end that has been reacted with a reactive moiety; and comparing the total number of chains in the sample to the number of chains labeled with the reactive moiety to quantify the amount of a 1,6 anhydro structural moiety in the sample, wherein the enoxaparin sample is not further digested or depolymerized during the above process, thereby analyzing the enoxaparin sample.