Patent ID: 8030076

Claim:
A method of producing a transformed soybean shoot comprising: (a) initiating the germination of a soybean seed; (b) isolating an embryonic axis including the embryonic meristem from the soybean seed to prepare an explant; (c) exposing the explant to a disarmed Agrobacterium vector comprising a heterologous genetic construct, wherein said genetic construct comprises a selectable marker gene, and said genetic construct is transferred into at least one cell in the meristematic region; (d) culturing the cells of the meristematic region in the presence of a selection agent to identify a transformed soybean cell that contains the genetic construct and in the presence of a shoot-inducing agent, wherein the selection agent is glyphosate and the shoot-inducing agent is only glyphosate; and (e) inducing formation of one or more shoots from the meristematic region, wherein said shoot comprises at least one transformed cell, to produce a transgenic soybean shoot.