Patent ID: 7993827

Claim:
A method to evaluate the integrity of chromatin/DNA of sperm cells of an animal comprising the following steps in sequence: a) treating a sample containing the sperm, with a solution of DNA denaturing solution, b) a single treatment step of treating the sample in the solution obtained in step a) with a single lysis solution to extract nuclear proteins of the sperm cells, wherein the DNA denaturing solution and the lysis solution are different and the lysis solution comprises sodium chloride, between 1 and 3M, dithiothreitol (DTT) between 0.001 and 2M 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris) between 0.001 and 2M and toctylphenoxyolyethoxyethanol (Triton X-100) between 0.1% and 3%, and c) evaluating the integrity of the chromatin/DNA of the sperm cells based on measurement of halo size of the sperm cells.