Patent ID: 8192960

Claim:
A method for replicating a DNA molecule, comprising subjecting a DNA molecule to a replication reaction in a replication reaction mixture, which replication reaction mixture comprises a minimal functional Pol III replicase, wherein said minimal functional Pol III replicase comprises a first component, which first component comprises an α subunit from a gram negative bacteria, wherein said α subunit comprises amino acid consensus sequence motifs A, B, and C arranged in the order C-A-B from amino terminus to carboxyl terminus, wherein said minimal functional Pol III replicase and said replication reaction mixture lack each subunit (τ/γ, δ and δ′) of the clamp loader, and wherein said DNA molecule is replicated in said replication reaction in said replication reaction mixture by said minimal functional Pol III at a rate of at least 100 nucleotides per second.