Patent ID: 7985783

Claim:
A method for modifying a polypeptide, the method comprising: contacting a non-naturally occurring, recombinant polypeptide comprising a heterologous, converted sulfatase motif generated by reaction with a formylglycine-generating enzyme (FGE) with a reactive partner comprising an aldehyde-reactive group and a moiety of interest, wherein the heterologous, converted sulfatase motif is less than 13 amino acid residues and contains a contiguous sequence of the formula: X 1 (FGly)X 2 Z 2 X 3 R (SEQ ID NO: 3) (I) wherein FGly is a 2-formylglycine residue; Z 2 is a proline or alanine residue; X 1 , X 2 and X 3 are each independently any amino acid; and wherein said contacting is under conditions sufficient for conjugation of the moiety of interest of the reactive partner to FGly of the polypeptide, thereby producing a modified polypeptide.