Patent ID: 8236562

Claim:
A method for preparation of ready-to-use cryoconserved mature dendritic cells, the method comprising: (a) providing immature dendritic cells: (b) culturing the immature dendritic cells in a culture medium containing a maturing cocktail comprising maturing stimulants IL-1β, IL-6, PGE 2 , and TNF-α to obtain mature dendritic cells, wherein said culture medium contains from 0.1 to 100 ng/ml IL-1β, from 0.1 to 100 ng/ml IL-6, from 0.1 to 1.0 μg/ml prostaglandin E 2 (PGE 2 ), and from 0.1 to 100 ng/ml TNF-α; and (c) freezing the mature dendritic cells at a concentration of from 5×10 6 cells/ml to 20×10 6 cells/ml in a freezing medium comprising: (i) autologous serum or autologous plasma; (ii) 5-25% DMSO; and (iii) 5-10% glucose, to obtain frozen dendritic cells suitable after thawing for administration to a patient.