Patent ID: 8394368

Claim:
A method for promoting survival of hematopoietic stem cells that have been transplanted to a patient, comprising: a process for separating mononuclear cells including lymphocytes from umbilical cord blood; activating and proliferating the lymphocytes with anti-CD3 antibody and/or interleukin-2; selecting CD4 positive cells from the activated and proliferated lymphocytes; activating and proliferating the selected CD4 positive cells with anti-CD3 antibody and/or interleukin-2; cryopreserving the activated and proliferated CD4 positive cells for use in the patient being transplanted with hematopoietic stem cells from umbilical cord blood and in need of the activated and proliferated CD4 positive cells; preparing a formulation for administration from the cryopreserved CD4 positive cells; and administering the formulation to the patient who recently received transplantation with hematopoietic stem cells from umbilical cord blood.