Patent ID: 8691595

Claim:
A method for detection of an analyte from a biological sample, comprising: a) contacting the biological sample with a reversible binding partner 1 immobilized on a solid phase, to which a first analyte binder is reversibly bound via a reversible binding partner 2, that is itself bound to the first analyte binder, whereby the first analyte binder is immobilized by way of the bond between the reversible binding partners 1 and 2, wherein the binding pair of reversible binding partners 1 and 2 is selected from one of the following binding pairs: i. positively- and negatively-charged peptide oligomers, ii. Ca 2+ -binding peptide/protein and antibodies that the peptide/protein binds with high affinity, when the peptide/protein has bonded Ca 2+ , iii. an oligohistidine and Ni—NTA, wherein the first analyte binder is labeled, and whereby the analyte is bound to the reversibly immobilized first analyte binder when the biological sample contains the analyte, b) separating the biological sample, c) adding a dissolution buffer, which breaks the bond between the reversible binding partners 1 and 2, whereby the binding of the analyte to the first analyte binder remains optional, and wherein the dissolution buffer further contains a second analyte binder labeled for detection in a sandwich immunoassay, whereby the first analyte binder is labeled and remains labeled with a label for the detection of analytes after the dissolution buffer is added, and d) detecting the analyte in the dissolution buffer in the case that the biological sample contains the analytes, and/or determining the absence of the analyte in the case that the biological sample does not contain the analytes, respectively.