Patent ID: 8780443

Claim:
Method for actuation control of a microscope having a common illuminating unit, a beam splitter for splitting light from the common illuminating unit into first and second illumination lights with intensities that can be specified according to an adjustable distribution ratio, a modulator for continuously, variably adjusting the intensity of the second illumination light, an imaging module, and a manipulating module, and wherein the first illumination light is used in the imaging module and moves at least in a first direction and the second illumination light is used in the manipulating module and moves at least in a second direction, the method comprising the steps of: determining a maximum value of the intensity of the second illumination light based on specified intensities for the first illumination light and the second illumination light, and on the intensity specified for the first illumination light being assigned a higher priority compared to the specified intensity for the second illumination light, controlling at least one of the distribution ratio by which the beam splitter splits the light from the common illuminating unit into the first illumination light and the second illumination light, and an intensity modulation of the second illumination light by the modulator, so that the first illumination light has an actual intensity equal to the specified intensity for the first illumination light and the second illumination light has an actual intensity equal to the maximum value, illuminating a sample with the first and second illumination lights, through a beam combiner; and detecting the light coming from the illuminated sample.