Patent ID: 8242242

Claim:
An artificial transcription factor polypeptide which artificially activates or represses gene expression in prokaryotes, wherein the artificial transcription factor comprises one to three zinc finger domains fused to an effector domain comprising at least one truncated form of a catabolite regulatory protein (CRP); wherein said truncated form of CRP is encoded by SEQ ID NO. 3 or encoded by SEQ ID NO. 5; wherein the zinc finger domain(s) is (are) selected from the group consisting of: Z1: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 13; Z2: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 15; Z3: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 17; Z4: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 19; Z5: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 21; Z6: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 23; Z7: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 25; Z8: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 27; Z9: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 29; Z10: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 31; Z11: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 33; Z12: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 35; Z13: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 37; Z14: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 39; Z15: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 41; Z16: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 43; Z17: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 45; Z18: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 47; Z19: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 49; Z20: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 51; Z21: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 53; Z22: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 55; Z23: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 57; Z24: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 59; Z25: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 61; and Z26: zinc finger domain encoded by nucleic acid sequence of SEQ ID NO. 63.