Patent ID: 7932076

Claim:
A composition comprising serum-free culture media, wherein said serum-free culture media provides a growth media that supports a dividing population of Treponema organisms in vitro under microaerophilic conditions wherein said serum-free culture media comprises: a) a sugar component comprising glucose, sucrose, fructose, lactose, and mannonse; b) a salt component comprising sodium chloride, potassium chloride, lithium chloride, calcium chloride, magnesium sulfate, potassium sulfate, sodium phosphate, ferrous sulfate, and sodium bicarbonate; c) a peptide component comprising peptone and neopeptone; d) an ionic component comprising manganese and magnesium, e) SITE +3 Liquid Media Supplement; f) N 1 Medium Supplement; g) RPMI medium; h) an amino acid component comprising essential amino acids, non-essential amino acids, L-methionine, and L-glutamine; i) pluronic F-68; j) Vanderzandt's supplement; k) sodium pyruvate; l) brain extract type 1; m) brain extract type 6; n) sphingomyelin; o) concentrated lipid solution; p) N-acetyl glucosamine; q) thyroxine; r) thyroid powder; s) estradiol; t) glycine; u) muscle extract powder, v) myelin basic protein, w) lactalbumin hydrolysate, x) granulated yeast y) brain heart infusion; and z) Hepes.