Patent ID: 7371565

Claim:
An intracellular-reaction measuring apparatus for measuring intracellular reactions after introduction of chemical substances by the use of a specimen in which a plurality of cells which stand adherent to one another, are contained, the apparatus comprising: photo-detecting means which detects the intensity of first light emitted from said cells in accordance with the presence of a stated protein and the intensity of second light emitted from said cells notwithstanding that a stated protein is present or not therein; a controller that selectively sets into a measuring optical path a first filter member for making the photo-detecting means detect said first light and a second filter member for making the photo-detecting means detect said second light; a first extracting member that extracts expressed cells emitting said first light as an image on the basis of a detection signal of said first light detected by said photo-detecting means when the controller sets the first filter into the measuring optical path; a second extracting member that extracts all cells emitting said second light as an image on the basis of a detection signal of said second light detected by said photo-detecting means when the controller sets the second filter into the measuring optical path; a specifying member that superposes the image of the expressed cells extracted by the first extracting means and the image of all cells extracted by the second extracting means and specifies a cell region in which said expressed cells extracted by the first extracting member are contained with a higher proportion than a stated standard, among cell regions containing said all cells inclusive of cells having no stated protein extracted by the second extracting member; and an analyzing member which analyzes said intracellular reactions on the basis of the detected signal, which is detected by said photo-detecting means, of said second light emitted from said cells in which said stated protein is present and the second light emitted, in accordance with intracellular reactions induced by the stated protein, from said cells in which said stated protein is not present.