Patent ID: 7332285

Claim:
A method for identifying one or more different target nucleotide sequences comprising: providing a sample potentially containing one or more target nucleotide sequences comprising sequence differences; performing a ligase detection reaction to form a ligation product comprising a first target specific portion complementary to the target nucleotide sequence and a second target specific portion complementary to the target nucleotide sequence, wherein the ligation product further comprises a 5′ upstream primer-specific portion and a 3′ downstream primer-specific portion, wherein the first target specific portion and the second target specific portion are located between the 3′ downstream primer-specific portion and the 5′ upstream primer-specific portion; providing an oligonucleotide primer set comprising (a) an upstream primer containing the same sequence as the 5′ upstream primer-specific portion of the ligation product sequence and (b) a downstream primer complementary to the 3′ downstream primer-specific portion of the ligation product sequence; performing a polymerase chain reaction to form extension products comprising the ligation product sequence and/or complements thereof; and, detecting the extension products to identify one or more target nucleotide sequences in the sample.