Patent ID: 7824532

Claim:
A disposable cassette for conducting electrophoresis, wherein the cassette comprises: i) an enclosed chamber having a top wall, a bottom wall, two side walls, a first end wall and a second end wall, wherein; the top wall comprises one or more apertures; the bottom wall is contiguous, and the chamber comprises a first region, a second region and a third region, wherein the second region is located between the first end wall and the third region, the first region is located between the second end wall and the third region, and the third region is located between the second region and the first region, and wherein the one or more apertures of the top wall are located above and adjacent to the third region; and wherein the chamber comprises an electrophoresis area; ii) an anode located within either the second region or the first region iii) a cathode located within either the second region or the first region, provided that the anode and the cathode are not together in the same region; and iv) an electrophoresis gel matrix, said electrophoresis gel matrix comprising one or more wells located below the apertures; wherein the electrophoresis gel matrix substantially occupies the first region, the second region and the third region, and wherein at least a portion of the anode is in contact with the electrophoresis gel matrix; and wherein the cassette further comprises a matrix, wherein the matrix is in contact with the cathode, and the matrix comprises at least one water sparingly soluble salt; and wherein during the electrophoresis the electrophoresis gel matrix comprises at least one water sparingly soluble salt ion.