Patent ID: 7459161

Claim:
A method for eliciting an immune response to a fusion protein in a subject comprising: administering to a subject a population of bacteria which produces and exports a fusion protein in an amount sufficient to elicit an immune response in said subject to said fusion protein, wherein said bacteria comprises an expression vector encoding said fusion protein, wherein said fusion protein comprises an export protein linked to a protein of interest in a 5′ to 3′ arrangement, wherein said export protein is Salmonella enterica serovar Typhi (S. Typhi) cytolysin A (ClyA) protein of SEQ ID NO: 2 or Escherichia coli hemolysin E (HlyE) protein encoded by the polynucleotide sequence set forth in SEQ ID NO:25, and wherein said bacteria is S. Typhi or E. coli ; thereby eliciting an immune response to said fusion protein in said subject.