Patent ID: 8323905

Claim:
A method of determining soluble GPVI (sGPVI) in a human body fluid using two or more anti-GPVI antibodies or fragments thereof having different binding sites, comprising: 1) obtaining a sample that is a sample of a human body fluid or a sample prepared from a human body fluid; 2) removing platelets from the sample, if the sample is a platelet containing sample; 3) contacting the sample with a first anti-GPVI antibody or fragment thereof; 4) contacting said sample with a second anti-GPVI antibody or fragment thereof, wherein the second antibody or fragment thereof has a GPVI binding site different from that of the first antibody or fragment thereof; and 5) determining a value of soluble GPVI (sGPVI) in said sample based on said contacting with the first anti-GPVI antibody or fragment thereof and said contacting with the second anti-GPVI antibody or fragment thereof, wherein at least two of anti-GPVI antibodies or fragments thereof selected from said two or more anti-GPVI antibodies or fragments thereof specifically bind loop 9 or loop 11 in GPVI domain 2, and loop 2 or loop 5 in GPVI domain 1 respectively.