Patent ID: 7723296

Claim:
A method of treating Pompe disease, said method comprising administering to a subject in need thereof an effective amount of modified acid α-glucosidase prepared by: (a) derivatizing an oligosaccharide comprising a phosphorylated mannose with a compound containing a carbonyl-reactive group; (b) oxidizing an acid α-glucosidase having at least one oligosaccharide to generate at least one carbonyl group on the at least one oligosaccharide of the acid α-glucosidase; and (c) reacting the derivatized oligosaccharide with the oxidized oligosaccharide of the acid α-glucosidase, thereby coupling the oligosaccharide to the acid α-glucosidase, wherein the modified acid α-glucosidase has more than about 0.9 moles of phosphorylated mannose per mole of acid α-glucosidase.