Patent ID: 8221612

Claim:
A method of determining concentration of glucose in a sample, the method comprising: applying the sample to a biosensor, wherein the biosensor comprises: a first and a second substrate comprising a reaction zone therebetween; a plurality of electrodes comprising a fill indicator electrode, a working electrode, and a counter or counter/reference electrode formed on the first substrate, wherein the reaction zone comprises an enzyme, a cofactor, and a mediator associated with at least the working electrode, wherein the mediator consists of one of the two following formulae: where X and Y can independently be oxygen, sulphur, CR 3 R 4 , NR 3 , or NR 3 R 4+ or the functional group CZ 1 Z 2 , where Z 1 and Z 2 are electron withdrawing groups; R 1 and R 2 can independently be a substituted or unsubstituted aromatic or heteroaromatic group; and R 3 and R 4 can independently be a hydrogen atom, a hydroxyl group or a substituted or unsubstituted alkyl, aryl, heteroaryl, amino, alkoxyl, or aryloxyl group; applying a potential between the working electrode and the reference/counter electrode within 3 seconds of the sample covering the plurality of electrodes; and measuring a current between the working electrode and the reference/counter electrode to determine the concentration of glucose in the sample.