Patent ID: 7049107

Claim:
A method of exchanging a base of a phospholipid comprising: producing phospholipase D (PLD)) enzyme from phospholipase-producing microorganisms selected from the genus Streptomyces : combining the PLD enzyme with a hydroxyl-containing compound and a phospholipid to form a reaction mixture; said phospholipid being dissolved in an organic solvent; adding an alcohol to the reaction mixture to create an aqueous/organic interphase; allowing the mixture to react for a time period sufficient to exchange the base; allowing the reaction mixture to separate into an aqueous phase and an organic phase following the exchange of the base; incubating the aqueous phase with phospholipid; separating the PLD enzyme from unreacted hydroxyl-containing compound; and reusing the separated hydroxyl-containing compound and the separated PLD enzyme in the combination step.