Patent ID: 6951715

Claim:
A method of producing a biosensor comprising: dispensing a liquid optical cement between a master grating structure and a substrate, wherein the master grating structure comprises features having a depth and a period; causing the liquid optical cement to harden forming a hardened liquid, wherein the hardened liquid adheres to the substrate; separating the substrate and the hardened liquid from the master grating structure, the hardened liquid replicating the master grating structure, the hardened liquid thus forming an optical grating that replicates the features of the master grating structure, depositing a coating onto the hardened liquid, a coating having a higher refractive index than the hardened liquid to form an optical grating; and immobilizing one or more specific binding substances on the optical grating form a biosensor; wherein, when the biosensor is illuminated, a resonant grating effect is produced on the reflected radiation spectrum, and wherein the depth and period of the optical grating are less than the wavelength of the resonant grating effect.