Patent ID: 8911939

Claim:
A method of determining quality of Sorghum bicolor seed production, the method comprising: a. providing a biological sample comprising Sorghum bicolor seeds, wherein the biological sample is suspected of also containing Johnsongrass or Johnsongrass hybrid genetic material; b. providing the primer pair of SEQ ID NOS.: 76 and 77 or the probe of SEQ ID NO: 136 that selectively binds to the Johnsongrass or Johnsongrass hybrid genetic material to discriminate against related genetic material; c. performing a hybridization or an amplification reaction wherein the primer pair of SEQ ID NOS.: 76 and 77 or probe of SEQ ID NO: 136 binds to Johnsongrass or Johnsongrass hybrid genetic material and does not substantially bind to genetic material from Sorghum bicolor ; and d. determining the quality of Sorghum bicolor seed production based on the presence or the amount of Johnsongrass or Johnsongrass hybrid genetic material.