Patent ID: 7670833

Claim:
A method of analyzing a plurality of molecular fractions from a population of molecules from or within the same or different origin(s) in high throughput manner, comprising the steps of: (1) fractionating each of said plurality of molecular fractions into a spectrum or a group of serial fractions containing sub-populations of serial fractionated molecules according to properties of said serial fractionated molecules in fractionating media wherein each of said spectrum or said group of serial fractions is assigned a primary designated order relating to said fractionating and a secondary designated order relating and corresponding to the same or different origin(s); (2) recovering the serial fractionated molecules from the fractionating media wherein each of the recovered serial fractionated molecules is accorded with the same primary and secondary designated orders assigned in step (1) to each of said group of serial fractions from which it is recovered; (3) arraying said recovered serial fractionated molecules onto a solid substrate according to their corresponding primary and secondary designated orders in step (2); (4) designing and selecting probing molecules that can interact and bind to the recovered serial fractionated molecules arrayed on the supporting material in step (3), followed by performing incubation or hybridization of the probing molecules with the recovered serial fractionated molecules arrayed on the supporting material in step (3) to generate presentation patterns; (5) analyzing and correlating the presentation patterns according to the corresponding primary and secondary designated orders of the arrayed recovered serial fractionated molecules, wherein said analyzing and correlating is performed by mathematic calculation, and computerized image and data analysis in a high throughput way to generate a database, wherein said correlating correlates each of the arrayed recovered serial fractionated molecules to said fractionating and said same or different origin(s); and (6) measuring the properties of said serial fractionated molecules; and wherein said method of analyzing is used for detection of nucleic acid, protein, or peptide, and said molecules are nucleic acids, proteins, or peptides.