Patent ID: 8476491

Claim:
A method of utilizing the pts gene and RNA interference of the ads gene to increase patchouli alcohol content in Artemisia annua L., said method comprising: a) obtaining a partial sequence of artemisinin biosynthesis pathway key enzyme ads gene having the sequence of SEQ ID NO: 1 that is capable of forming a hairpin structure, and the complete open reading frame sequence of the patchouli alcohol biosynthesis pathway key enzyme pts gene having the sequence of SEQ ID NO: 2 that is linked to the nucleotide sequence encoding the plastid targeted signal peptide of the Arabidopsis tp gene having the sequence of SEQ ID NO: 3; b) obtaining a plant expression vector comprising the partial sequence of the ads gene and a plant expression vector comprising the pts open reading frame linked to the nucleotide sequence encoding the plastid targeted signal peptide; c) transforming Agrobacterium tumefaciens simultaneously with the plant expression vectors; d) transforming Artemisia annua L. plants with the vector-transformed Agrobacterium tumefaciens obtained in step c) and analyzing the transformed plants with PCR to identify transgenic Artemisia annua L. plants; and e) determining the patchouli alcohol content in the transgenic Artemisia annua L. plants.