Patent ID: 7867778

Claim:
A method for loading a sample for imaging by an optical tomography system comprising the steps of: loading a sample volume including at least one microscopic sample into a sample delivery tube; coupling the sample delivery tube to a hydrodynamic focus cell with a nozzle injector, where the hydrodynamic focus cell includes a plurality of sheath injection ports and a capillary tube outlet; injecting a sheath fluid into the plurality of sheath injection ports to form separate sheath flow streams and delivering the sample volume through the nozzle injector while maintaining a distance between an output end of the plurality of sheath injection ports and an output of the nozzle injector so that the sheath fluid and sample flow streams join coaxially at the output end of the nozzle injector to create a coaxially joined sheathed sample volume; while injecting, simultaneously and continuously axially rotating a capillary tube inserted into a rotating seal coupled to receive the coaxially joined sheathed sample volume; and impelling the coaxially joined sheathed sample volume through the rotating seal into the continuously axially rotating capillary tube, where the continuously axially rotating capillary tube has a smaller cross-sectional area than tapered flow cell at the position of the sample injector, and where the hydrodynamic focus cell includes a tapered flow cell so that a reduced volume of the at least one microscopic sample and sheath fluid is constrained to a central region within the rotating capillary tube, and where the plurality of sheath injection ports, the sample delivery tube, the rotating capillary tube and the continuously axially rotating seal are axially aligned along a central axis and the continuously axially rotating capillary tube is rotated with the rotating seal around the central axis; wherein a rotation bearing allows the capillary tube to be rotated via a belt and motor.