Patent ID: 8883487

Claim:
An integrated single-use device for performing a nucleic acid analysis, comprising: a housing, an entry port for accepting a sample suspected of containing a target nucleic acid, a first chamber operably connected to the entry port and comprising: a reagent for extracting the target nucleic acid, magnet beads comprising a coating configured to bind the target nucleic acid, and a wax configured to melt and form a contiguous wax layer in a region of egress of the first chamber; a means for generating a magnetic field configured to draw the magnet beads through the contiguous wax layer; a first conduit permitting passage of extracted nucleic acid from the region of egress of the first chamber into an amplification chamber, said housing containing an amplification reagent that is capable of incorporating a detectable label into an amplified nucleic acid target, the amplification chamber having a heating means and a temperature sensing means for controlling amplification conditions, the amplification chamber being operably linked to a second conduit containing a sensing region with an immobilized capture oligonucleotide, a means for moving the amplified target to the sensing region to permit binding of said amplified target to said capture oligonucleotide, and a holding chamber operably attached to said second conduit and containing a fluid configured to substantially displace uncaptured amplified target from the sensing region and permit sensing of said detectable label retained in said sensing region.