Patent ID: 7381538

Claim:
A distal tip of a biosensor ion sensing transducer for use in detecting an analyte comprising a halogenated organic compound, the tip comprising: a biocomponent comprising at least one enzyme selected from the group consisting of hydrolases, subclass EC 3.8, and lyases, subclass EC 4.5, for carrying out a dehalogenation of the compound, said biocomponent immobilized to a surface of the tip by means selected from the group consisting of (a) entrapment within a hydrogel; (b) entrapment within a polymeric network; (c) encapsulation; (d) covalent-bonding; and (e) adsorption; wherein said biocomponent has been treated to maintain a period of enzymatic efficacy; and wherein said biocomponent is stabilized to said tip by means selected from the group consisting of crosslinking a surface of said immobilized biocomponent, crosslinking a polymer layer to said biocomponent, adding a gel-hardening agent to said biocomponent, adding a stabilizing agent to said biocomponent, and modifying a component used to immobilize said biocomponent to said surface of the tip.