Patent ID: 8263393

Claim:
A method for expressing a heterologous polypeptide in a cell, said method comprising: culturing a transfected cell clone to allow expression of the heterologous polypeptide, wherein cells of the transfected cell clone contain integrated into their genomes a transfected nucleic acid molecule comprising: a promoter functionally linked to an open reading frame encoding the heterologous polypeptide, said promoter comprising a human cytomegalovirus, a simian virus 40, an ubiquitin C, or an elongation factor one-alpha promoter, and a binding site for a p300/CBP protein, a P/CAF protein, or CBP, and wherein the cells of said transfected cell clone comprise said p300/CBP protein, P/CAF protein, or CBP, wherein the transfected nucleic acid molecule comprising the promoter functionally linked to an open reading frame encoding the heterologous polypeptide is flanked on one or both sides by a Stabilizing Anti-Repressor (STAR) molecule comprising a nucleic acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7.