Patent ID: 7858343

Claim:
A method for producing a protein, the method comprising the steps: a) preparing host cells from at least one yeast type of genera Saccharomyces, Schizosaccharomyces, Kluyveromyces, Hansenula, Pichia, Arxula, Schwanniomyces, Candida , and Yarrowia , which along with their at least one, own, homologous DNA sequence coding for at least a first calnexin, also contain in each case, at least one recombinant DNA sequence coding for the protein, as well as at least one additional recombinant DNA sequence coding for a second calnexin, wherein the protein in the host cell represents a heterologous, secretable protein; b) causing the host cells, with the help of at least one sequence coding for the protein, to express the heterologous protein, and, with at least one additional sequence coding for at least a second calnexin, to overexpress calnexins; c) causing the host cells to secrete the heterologous protein, and d) separating the secreted heterologous protein.