Patent ID: 8298768

Claim:
A method of determining sequence of a target nucleic acid comprising: (a) sequencing the target nucleic acid to produce primary sequence information for the target nucleic acid; (b) identifying missing sequences and/or low confidence sequences in the target nucleic acid from the primary sequence information determined in step (a); (c) synthesizing a plurality of target-specific oligonucleotides, wherein each of said plurality of oligonucleotides corresponds to at least one of the sequences identified in step (b); (d) selecting, from a library of fragments of the target nucleic acid, fragments that hybridize with the target-specific oligonucleotides synthesized in step (c); (e) sequencing fragments selected in step (d) to produce sequence information for the selected fragments; and (f) assembling sequence information for the selected fragments determined in step (e) with the primary sequence information determined in step (a) to produce an assembled sequence, thereby determining sequence of the target nucleic acid.