Patent ID: 8409809

Claim:
A method of detecting a mutation at position 4321 in the nucleic acid molecule of SEQ ID NO: 1 or 2 in a sample, the method comprising: (a) contacting a sample with a probe consisting of 10 to 50 nucleotides for the detection of said mutation, wherein said probe hybridizes under high stringency conditions to any of the polynucleotide sequences (i), (ii), (iii), (iv), or (v): (i) a polynucleotide sequence selected from nucleotides 1 to 9104 of SEQ ID NO: 1 or 2; (ii) a polynucleotide sequence selected from nucleotides 1 to 7584 of SEQ ID NO: 1 or 2; (iii) a polynucleotide sequence selected from nucleotides 1 to 7581 of SEQ ID NO: 1 or 2; (iv) a polynucleotide sequence selected from a nucleotide sequence coding for the protein sequence of SEQ ID NO:8 or 9 or for the protein sequence of SEQ ID NO:8 or 9 containing at least one of the mutations depicted in SEQ ID NO: 1 or 2; or (v) a polynucleotide sequence selected from a nucleotide sequence complementary to any of the nucleotide sequences of (i), (ii), (iii), or (iv): wherein the polynucleotide sequence codes for the protein sequence of SEQ ID NO. 8 or 9 containing a mutation at position 1441, and wherein said high stringency conditions comprise hybridization at 68° C. in a solution comprising 50% formamide, 5×SSC (Sodium and sodium Citrate buffer) or 5× SSPE (Sodium, Sodium Phosphate, and EDTA buffer at pH 7.7), 5× Denhardt's solution, 1% Sodium Dodecyl Sulfate (SDS), and 100 μg/ml denatured salmon sperm DNA, followed by washing at 68° C. in a buffer comprising 0.2 SSC and 0.1% SDS, and (b) detecting the presence of the mutation in the nucleic acid of the sample.