Patent ID: 7956173

Claim:
An isolated, non-naturally occurring nucleic acid molecule that encodes an immunoglobulin heavy chain or an immunoglobulin light chain of an antibody or antigen binding fragment thereof that binds sphingosine-1-phosphate (S1P), wherein: A. when the nucleic acid molecule encodes an immunoglobulin heavy chain, wherein the immunoglobulin heavy chain is selected from the group consisting of: (i) an immunoglobulin heavy chain that comprises a first sequence of amino acid residues of sequence DHTIH (SEQ ID NO: 13), a second sequence of amino acid residues AISPRHDITKYNEMFRG (SEQ ID NO: 31), and a third sequence of amino acid residues of sequence GGFYGSTIWFDF (SEQ ID NO: 15); (ii) an immunoglobulin heavy chain that comprises a heavy chain variable domain comprising a sequence of amino acid residues having an amino acid sequence: EVQLVQSGAEVKKPGESLKISCQSFGYIFIDHTIHWMRQMPGQGLE WMGAISPRHDITKYNEMFRGQVTISADKSSSTAYLQWSSLKASDT AMYFCARGGFYGSTIWFDFWGQGTMVTVSS (SEQ ID NO: 32, residues 20-140, inclusive); and (iii) an immunoglobulin heavy chain that comprises a sequence of amino acid residues having an amino acid sequence: (SEQ ID NO: 38) EVQLVQSGAEVKKPGESLKISCQSFGYIFIDHTIHWMRQMPGQGLEWMGA ISPRHDITKYNEMFRGQVTISADKSSSTAYLQWSSLKASDTAMYFCARGG GFYGSTIWFDFWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCL VKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGT QTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFPP KPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQ YNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPRE PQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTP PVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSP GK, residues 20-455, inclusive); and and B. when the nucleic acid molecule encodes an immunoglobulin light chain, the immunoglobulin light chain is selected from the group consisting of: (i) an immunoglobulin light chain that comprises a first sequence of amino acid residues of sequence ITTTDIDDDMN (SEQ ID NO: 10), a second sequence of amino acid residues of sequence EGNILRP (SEQ ID NO: 11), a third sequence of amino acid residues of sequence LQSDNLPFT (SEQ ID NO: 12); (ii) an immunoglobulin light chain that comprises a light chain variable domain comprising a sequence of amino acid residues having an amino acid sequence: ETTVTQSPSFLSASVGDRVTITCITTTDIDDDMNWFQQEPGKAPKLL ISEGNILRPGVPSRFSSSGYGTDFTLTISKLQPEDFATYYCLQSDNL PFTFGQGTKLEIK (SEQ ID NO: 33, residues 21-127, inclusive); and (iii) an immunoglobulin light chain that comprises a sequence of amino acid residues having an amino acid sequence: (SEQ ID NO: 37) ETTVTQSPSFLSASVGDRVTITCITTTDIDDDMNWFQQEPGKAPKLLISE GNILRPGVPSRFSSSGYGTDFTLTISKLQPEDFATYYCLQSDNLPFTFGQ GTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKV DNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQG LSSPVTKSFNRGEC, residues 21-234, inclusive).