Patent ID: 8124349

Claim:
A method of identifying a compound that modulates binding of a cognate ligand to Toll-like receptor 2 (TLR2), the method comprising: (a) providing a sample comprising a chimeric polypeptide comprising a TLR2 polypeptide or ligand-binding fragment thereof, and a fluorescent protein; (b) contacting the sample with a cognate TLR2 ligand and a test compound, thereby forming a test sample; (c) incubating the test sample for a time and under conditions sufficient for the TLR2 ligand to bind to the TLR2 polypeptide in the absence of the test compound; and (d) determining binding between the TLR2 polypeptide and the TLR2 ligand in the test sample, wherein the TLR2 ligand is bound to a bead and wherein a difference in binding in the test sample compared to a control indicates that the test compound is a candidate compound for modulating binding of a cognate ligand to TLR2.