Patent ID: 8735131

Claim:
A method for designing a mutant protein deamidase having modified substrate specificity or improved oxidative stability compared to the protein deamidase to be mutated comprising the following steps: (A) identifying one or more amino acids in the amino acid sequence for a protein deamidase which correspond to the amino acids at positions 35, 38 to 43, 45, 46, 49, 79 to 84, 103 to 106, 117, 142, 143, 146, 166, or 185 in the amino acid sequence set forth in SEQ ID NO: 2; and (B) constructing a mutant amino acid sequence of the protein deamidase by substituting the one or more amino acids identified in step (A) with another amino acid or other amino acids or by deleting the one or more amino acids identified in step (A) thereby designing a mutant protein deamidase having modified substrate specificity or improved oxidative stability wherein the protein deamidase to be mutated has 90% or more identity to the amino acid sequence set forth in SEQ ID NO: 2.