Patent ID: 8465915

Claim:
A method for identification and isolation of transcription termination sequences from a plant comprising the steps of: i) providing a screening construct or screening vector comprising a) a promoter sequence, b) one or more insertion sites for insertion of DNA sequences, and c) at least one additional sequence which causes upon expression under said promoter sequence a readily detectable characteristic, wherein insertion of an efficient transcription terminator into said insertion site changes expression of said additional sequence by said promoter sequence in comparison to no insertion, ii) providing one or more DNA sequences to be assessed for their transcription termination capability by a) identifying intergenic regions between paired genes having an intergenic distance of about 400 to 3,000 base pairs from a plant, b) identifying one or more DNA sequences which are flanked on both sides by genes having at least 5% of expression level of actin in said plant, and c) isolating the one or more DNA sequences identified in step b), iii) inserting one or more copies of said DNA sequences obtained from step ii) into said insertion site of said screening construct or screening vector, iv) introducing said screening construct or screening vector with said inserted DNA sequences into an in vitro or in vivo transcription system suitable to induce expression from said promoter sequence, v) identifying and/or selecting screening construct or screening vector with a changed readily detectable characteristic in comparison to no insertion, and vi) isolating the inserted DNA sequences from said identified and/or selected screening construct or screening vector for use as transcription termination sequences and, optionally, determining their sequence.