Patent ID: 7400980

Claim:
A method for detecting DNA variation in a sample DNA sequence, comprising: aligning trace data of the sample DNA sequence to trace data of a reference DNA sequence to produce an aligned sample DNA sequence with frame numbers, whereby the trace data of the reference DNA sequence and the trace data of the sample DNA sequence are traces of data for each base in a DNA sequence; inputting the trace data of the bases of both the reference DNA sequence and the aligned sample DNA sequence for each particular frame number into a non-linear mathematical function of an anti-correlation calculation scheme for all the frame numbers to produce output values which are minimal values at each particular frame number for DNA base trace data of the aligned DNA sequence which does not have a variation as compared to the reference DNA sequence at the same base and to produce output values which are above the minimal values at the particular frame number for DNA base trace data of the aligned sample DNA sequence which do have a variation as compared to the reference DNA sequence at the same base; and outputting the produced minimal and above minimal values as indications of DNA variation.