Patent ID: 8653251

Claim:
A kit for synthesizing asRNA molecules and/or cDNA molecules from a sRNA, the kit comprising: a) a first primer complementary to a 3′ end of a first sRNA, the first primer having a 5′ extension comprising a nucleotide sequence corresponding to a partial 3′ end of the non-template strand of a consensus RNA polymerase recognition sequence and extending less than 19 nucleotides, for synthesizing a second sRNA from the first sRNA which has a 3′ end comprising a partial 5′ end of a template strand of the consensus RNA polymerase recognition sequence less than 19 nucleotides in length; b) a second primer comprising a nucleotide sequence corresponding to a most 5′ end of a non-template strand of the consensus RNA polymerase recognition sequence, wherein the second primer is of sufficient length to anneal to the consensus RNA polymerase recognition sequence at the 3′ end of the second sRNA and provide a complete non-template strand of the consensus RNA polymerase recognition sequence upon reverse transcription of the second sRNA using the second primer; and c) instructional materials for synthesizing asRNA and/or cDNA molecules from the first sRNA using the first primer and the one or more second primers.