Patent ID: 7135329

Claim:
An immunochemical assay device comprising a base member and at least one reservoir pad disposed on said base member and having sufficient porosity and volume to receive and contain a liquid; at least one wicking membrane disposed on said base member and distal to said at least one reservoir pad, said at least one wicking membrane having sufficient porosity and volume to absorb and to wick a substantial proportion of the sample received in said at least one reservoir pad; and at least one first filter zone which is separate and distinct from said at least one reservoir pad and said at least one wicking membrane, and interposed between and contiguous with each one of said wicking membrane and said reservoir pad, said first filter zone having impregnated therein one or more labeled immunochemical components capable of binding to or competing with one or more analytes of interest in said sample to form one or more immuno-complexes, said one or more labeled immunochemical components comprises an antibody specific for amino acids 26–51 of NT-proBNP (SEQ ID No:1) conjugated with a detectable label and biotinylated monoclonal antibody specific for amino acids 1–25 of NT-proBNP (SEQ ID NO:1); at least one second filter zone which is separate and distinct from said at least one reservoir pad, said wicking membrane and said first filter zone, and contiguous with each one of said wicking membrane, and said first filter zone, said second filter zone having sufficient porosity and volume to receive and containing a liquid sample on which the assay is to be performed; at least one additional immobilized immunochemical component disposed in at least one assay indicia zone of each one of said at least one wicking membrane downstream of said reservoir pad and defining assay indicia, said at least one additional immobilized immunochemical comprises an antibody specific for amino adds 1–25 of NT-proBNP (SEQ ID NO:1) and/or streptoavidin.