Patent ID: 7378265

Claim:
A viral preparation comprising non-enveloped viruses obtained according to the method of preparation comprising: a) producing the viral preparation in an appropriate cell line; b) harvesting the viral preparation from the producer cell line and/or the culture supernatant; c) optionally, breaking the cells of the producer cell line; d) optionally clarifying; e) inactivating enveloped viruses in the said viral preparation in which a sufficient quantity of a solvent comprising tri-n-butyl phosphate (TNBP) of between 0.1% and 0.6% (volume/volume) and TWEEN® 80 at a concentration of between 0.5% and 2% (volume/volume) are introduced into said viral preparation and said solvent and TWEEN® 80 are allowed to act at a temperature between +4°C. to +37° C., at a pH of between 6.5 to 8.5 for a period which is sufficiently long to significantly reduce the quantity of enveloped viruses present in the said viral preparation, wherein said inactivation is capable of preserving at least 80% of the infectious activity of said non-enveloped viruses; and f) optionally purifying.