Patent ID: 8447133

Claim:
A method for determining the distortion of an image of an object from sub-image distortions, comprising: acquiring a first image; determining the centre of the image by the intersection of the particle-optical axis and the image plane; defining a sub-image adjacent to the centre of the image, the sub-image including a portion of the object; shifting the object; acquiring a series of images, the object being shifted between each acquisition; determining a shift between each acquisition of the series of images with respect to the first image and/or between each subsequent image; identifying a sub-image in each acquisition of the series of images from each known shift, each sub-image including the portion of the object; determining the sub-image distortions of each acquisition of the series of images with respect to each other; and determining the distortion of the first image from the determined sub-images sub-image distortions.