Patent ID: 6864060

Claim:
A yeast cell comprising: (i) a heterologous G protein coupled receptor; and (ii) a non-naturally occurring G protein subunit, wherein the G protein subunit is a mammalian Gα protein subunit comprising a mutation selected from the group consisting of: human Gα16(S270P); rat Gαs(D229S); rat Gαs(D229V); rat Gαs(N254D); rat Gαs(S286P); rat Gαs(E10K); mouse Gαi2-GαoB(S280P); human Gαl2(Q229L); human Gαl2(G228A); and human Gαi2(S288P); such that expression of said non-naturally occurring G protein subunit functionally integrates said heterologous G protein coupled receptor into the yeast cell pheromone signaling pathway.