Patent ID: 8338093

Claim:
A method for synthesizing primers from an array of polymers on a solid substrate, said method comprising the steps of: providing a solid substrate, wherein said substrate has a density, wherein a plurality of different known oligonucleotides sequences are located in a plurality of different, known locations on a surface of said substrate; attaching a plurality of linkers to the substrate, wherein the plurality of linkers comprises a photogroup, wherein the photo group is a compound selected from the group consisting of compounds represented by the following structural formula: wherein: X is an oligonucleotide or a peptide; R 1 and R 2 are H, or form a fused aryl ring; R 3 is H or aryl; and Ar is aryl; wherein the plurality of linkers comprises: a first group of linkers having a first cleavable moiety, wherein said first cleavable moiety is activatable under specific conditions and wherein activation of said first cleavable moiety disrupts the first group of linkers to provide a substrate with a plurality of attached first group of linkers, and a second group of linkers, wherein said second group of linkers does not comprise the first cleavable moiety; attaching a first nucleotide to at least one of said plurality of attached first and second groups of linkers to provide an attached first nucleotide; attaching a second nucleotide to a least one of said attached first nucleotide or said plurality of attached first and second groups of linkers to provide an attached nucleotide; attaching a third nucleotide to a least one of said attached first nucleotide, said second nucleotide or said plurality of attached first and second groups of linkers to provide an attached third nucleotide; repeating said steps of attaching nucleotide until the desired array of polymers of defined primers is complete, thereby synthesizing said primers comprising said first group of linkers and said primers comprising said second group of linkers, one nucleotide at a time on the array; subjecting the array to specific conditions to cleave the first group of linkers from said array, wherein said primers comprising said second group of linkers are retained on the substrate; identifying at least one known, different oligonucleotide sequence of said primer comprising said second group of linkers by determining said known location of said primer comprising said second group of linkers; and performing a MALDI-TOF mass spectrometry on said primer comprising said second group of linkers on said substrate to determine whether a result from said MALDI-TOF mass spectrometry confirms said identified known, different oligonucleotide sequence.