Patent ID: 8389278

Claim:
A method for differentiating human monocytic dendritic cell precursors (CD14 + CD1a − ) into immature dendritic cells having CD1a and having no CD14 on their cell surface, comprising: a) providing a cell population comprising non-activated human monocytic dendritic cell precursors (CD14 + CD1a − ); b) contacting the non-activated monocytic dendritic cell precursors in a low cellular avidity culture vessel with a dendritic cell culture media supplemented with at least 1% animal or human protein, and an effective amount of granulocyte-macrophage colony stimulating factor in the absence of additional cytokines for a time period sufficient for the human monocytic dendritic cell precursors to differentiate into immature dendritic cells having no expression of CD14 and having increased expression of CD1a on their cell surface.