Patent ID: 7229760

Claim:
A method for the amplification of mRNA of a sample comprising: i. generating cDNA from polyadenylated RNA employing at least one primer hybridizing to said polyadenylated RNA and comprising a 5′ poly(C) or a 5′ poly(G) flank wherein the concentration of said at least one primer is in the range of 10 μM to 60 μM; ii. 3′ tailing of said generated cDNA with a poly(G) tail when at least one of said primers comprises a 5′ poly(C) flank or with a poly(C) tail when at least one of said primers comprises a 5′ poly(G) flank; and iii. amplifying the tailed cDNA with a primer hybridizing to the tail(s) generated in ii. to obtain amplified cDNA; wherein said at least one primer in step i. comprises SEQ ID NO: 10.