Patent ID: 7285219

Claim:
A method for separating a component of interest from a fluid comprising (a) applying to a chromatographically active separation member comprising (i) a substrate having first and second opposed surfaces; and (ii) a continuous film of a chromatographically active functional polymeric material carried by said first surface, said continuous film having a thickness of from about 0.25 micron to about 3.0 microns; a fluid containing at least one component of interest, said chromatographically active functional polymeric material being capable of capturing said component of interest; wherein at least a portion of said component of interest is bound to said chromatographically active functional polymeric material carried by said first surface; (b) removing said fluid from said chromatographically active separation member having bound thereto said component of interest; (c) releasing said component of interest from said chromatographically active separation member; (d) applying an equilibration fluid to said separation member; and (e) repeating steps (a)-(d) at least one additional time.