Patent ID: 8753393

Claim:
A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps: fixation of the biological tissue via a treatment with a solution containing about 0.5% v/v glutaraldehyde in phosphate buffer at room temperature and a pH of 7.4; detoxification of the fixed biological tissue via a treatment with a solution containing about 0.70% w/v taurine in phosphate buffer at a temperature between 40° C. and 50° C. and a pH about 7; and washing of the detoxified fixed biological tissue in phosphate buffer at room temperature and a pH of 7; wherein the detoxification of the fixed biological tissue at a temperature greater than room temperature reduces the number of free aldehyde groups present on the fixed biological tissue when compared to detoxification of fixed biological tissue at room temperature.