Patent ID: 7828782

Claim:
An apparatus configured to form a closed cell culture system around a tissue defect comprising: (a) a means for sealing the tissue defect on a body surface or inside a body to form a closed cell culture system around the tissue defect; (b) a first disc having a through-hole; (c) a second disc being spaced parallel from the first disc and having a hollow adapter configured for jointing one end of a tube, wherein the hollow adapter of the second disc passes through the through-hole of the first disc; wherein a first space is defined between the surface of the tissue defect and the second disc, and a second space is defined between the second disc and the first disc, the first space being in fluid communication with the second space; (d) a perfusion introduction tube; and (e) a perfusion discharge tube; wherein the perfusion introduction tube is jointed at one end with the hollow adapter of the second disc and is capable of introducing a solution for maintaining a cell culture into the first space; and wherein the perfusion discharge tube is capable of discharging fluid from the second space to an area external to the means for sealing.