Patent ID: 7608752

Claim:
A method for gene targeting in a plant cell comprising: (a) providing the plant cell, wherein the plant cell comprises a target polynucleotide endogenous to the plant cell, wherein the target polynucleotide is a non-coding region of the genome; (b) providing a non-circular targeting vector to the plant cell, wherein the targeting vector comprises a target modifying polynucleotide and a geminiviral origin of replication, wherein the target modifying polynucleotide has at least 75-150 bp of homology to the target polynucleotide; (c) providing an appropriate viral replicase, wherein the replicase binds to the geminiviral origin of replication to stimulate replication of the targeting vector, whereby homologous recombination between the target modifying polynucleotide and the target polynucleotide produces a modified target polynucleotide; and (d) recovering a plant cell comprising the modified target polynucleotide integrated into its genome.