Patent ID: 8802415

Claim:
A process for production of a Talaromyces emersonii transformant, said process comprising the steps of: (a) providing one or more expression cassettes comprising at least one polynucleotide encoding cellulose, hemicellulase, and/or pectinase, and at least one promoter for expression of said polynucleotide; (b) providing a selection marker included in the expression cassette of (a) and/or included in a dedicated selection marker polynucleotide; (c) transfecting a Talaromyces emersonii host which endogenously produces cellulase and/or hemicellulase with the at least one expression cassette from (a) and the selection marker from (b); (d) selecting the Talaromyces emersonii transformant which comprises said at least one expression cassette from (a) and the selection marker from (b); and (e) isolating said Talaromyces emersonii transformant.