Patent ID: 8748101

Claim:
A method of detecting or predicting a liver condition, comprising: generating a microRNA profile from a biological sample, wherein the microRNA profile comprises the amounts of at least two specific microRNA sequences, by: receiving the biological sample, the sample being a tissue, serum, or plasma; and isolating microRNA from the biological sample by extracting the microRNA with an organic solvent and obtaining an aqueous phase containing the microRNA, then purifying the aqueous phase through a silica membrane to isolate the microRNA; using hybridization to identify the microRNA sequences; and measuring the amount of at least two specific microRNA sequences; and comparing the amounts of the at least two specific microRNA sequences to a reference to provide information for detecting or predicting the liver condition; wherein the at least two specific microRNA sequences are hsa-miR-122 and hsa-miR-486-3p.