Patent ID: 8101212

Claim:
A method of preparing a bioactive botanical cosmetic composition, said method comprising: providing a plant cell juice, said plant cell juice having been extracted from a fresh plant biomass, wherein said fresh plant biomass is from a plant selected from the group consisting of Calendula officinalis, Medicago sativa, Lavandula angustifolia, Salvia officinalis and Hordeum vulgare; treating the plant cell juice under conditions effective to separate the plant cell juice into a membrane fraction and a cell juice supernatant; and converting the membrane fraction under conditions effective to yield a stable bioactive botanical cosmetic composition exhibiting antiproteolytic, cell growth inhibition activity, and/or both antiproteolytic and cell growth inhibition activities, wherein said antiproteolytic activity is due to inhibition of at least one proteinase and said cell growth inhibition activity is due to inhibition of cell growth of at least one type of cell and wherein said converting comprises: stabilizing the membrane fraction component by mixing a non-ionic emulsifier and at least one antioxidant with the membrane fraction to yield a stabilized membrane fraction component; mixing the stabilized membrane fraction component with a polymer matrix to yield a membrane fraction matrix; and neutralizing the membrane fraction matrix to yield a bioactive botanical cosmetic ingredient having antiproteolytic activity, cell growth inhibition activity, or both antiproteolytic and cell growth inhibition activities, wherein said converting is performed prior to any aggregation of the membrane fraction.