Patent ID: 8715957

Claim:
A non-naturally occurring microbial organism having a 1,3-butadiene pathway, wherein said microbial organism comprises the following set of said 1,3-butadiene pathway enzymes (i) acrylyl-CoA acetyltransferase, (ii) 3-oxopent-4-enoyl-CoA reductase, (iii) 3-hydroxypent-4-enoyl-CoA transferase, synthetase or hydrolase, and (iv) 3-hydroxypent-4-enoate decarboxylase, and wherein said microbial organism comprises at least one exogenous nucleic acid encoding an enzyme from said 1,3-butadiene pathway enzymes (i)-(iv) wherein said enzyme is expressed in a sufficient amount to produce 1,3-butadiene.