Patent ID: 8062897

Claim:
A method for the in situ detection of one or more nascent RNA species in a human paraffin embedded tissue sample comprising the steps of: a) performing autofluorescence reduction on the human paraffin embedded tissue sample by pressure cooking the tissue sample at a temperature of about 125° C., at a pressure of between 20 to 24 PSI and for a period of 10 minutes to 1 hour to produce a pressure cooked sample, and then treating said pressure cooked sample with ammonia-ethanol at a concentration of 0.1% to 0.5% for between 10 and 50 minutes and sodium borohydride at a concentration of 1% to 5% for between 10 and 50 minutes in any order; b) hybridizing at least one labeled nucleic acid probe specific for one or more nascent RNA species to the tissue sample; c) detecting the at least one labeled nucleic acid probe in the tissue sample thereby detecting the presence of the one or more nascent RNA species.