Patent ID: 7405044

Claim:
A process for simultaneous quantitation of a human mitochondrial DNA and at least one of human nuclear DNA and human Y chromosome DNA in a sample, said process comprising the steps of: performing multiplex polymerase chain reaction for simultaneously amplifying a region of the human mitochondrial DNA containing a human/chimp mismatch and a mitochondrial DNA/nuclear DNA mismatch by using a primer pair specific to human mitochondrial DNA, the region of the human mitochondrial DNA being not the hypervariable region, and at least one region of the human nuclear DNA containing a duplicated region of the Yb lineage of Alu subfamilies and an AluYb8 diagnostic base by using a primer pair specific to human nuclear DNA, and a region of the human Y chromosome DNA containing a 90 base pair sequence which is deleted on the human X-chromosome in an X-Y chromosome homologous region by using a primer pair specific to the human Y chromosome DNA in order to obtain an amplified products; and quantitating the human mitochondrial DNA, and at least one of the human nuclear DNA and the human Y chromosome DNA by comparing the amplified products with a reference standard.