Patent ID: 6939676

Claim:
A method for selecting a cell transformed with one or more nucleic acid coding sequences comprising: (a) transforming a population of cells with a foreign DNA construct comprising at least one first nucleic acid coding sequence and a second nucleic acid coding sequence encoding a temperature sensitive marker protein selected from the group consisting of heat shock proteins, heat shock transcription factors, cold regulated proteins, and cold regulated protein transcription factors, each of said first and second nucleic acid coding sequences being operatively linked to gene expression control sequences, said transforming generating a second population of cells comprising cells which have been successfully transformed with said foreign DNA construct and non-transformed cells, (b) selecting said cells which have been successfully transformed from said non-transformed cells in said second population of cells from said transforming in (a), said selecting consisting essentially of culturing said second population of cells under temperature conditions wherein growth of said non-transformed cells is suppressed or prevented while growth of said cells which have been successfully transformed is supported, and (c) recovering cells from (b) exhibiting significant growth, wherein the recovered cells exhibiting significant growth are said cells which have been successfully transformed.