Patent ID: 8497364

Claim:
A method of forming an inactivator dsRNA, the method comprising: contacting a sample with an initiator, wherein the sample comprises a detection target, wherein the initiator comprises: a) a detection target binding region and b) an initiator region, wherein the detection target binding region can interact with the detection target and wherein the initiator region is not exposed prior to the detection target binding region interacting with the initiator; contacting the sample with a first substrate monomer that comprises: i) a silencing target complement region, ii) an initiator complement region, wherein the initiator complement region hybridizes to the initiator region of the initiator, wherein upon binding of the detection target binding region to the detection target, the initiator region of the initiator is exposed and binds to the initiator complement region of the first substrate monomer, and iii) a substrate monomer complement region; and contacting the sample with a second substrate monomer that comprises: i) a substrate monomer region that hybridizes to the substrate monomer complement region, and ii) a silencing target region that hybridizes to the silencing target complement region; wherein upon binding of the initiator complement region of the first monomer to the initiator region of the initiator, the silencing target complement region hybridizes to the silencing target region to form an inactivator dsRNA.