Patent ID: 8211685

Claim:
A polypeptide comprising residues 1-95, and optionally residues 96-109, of the sequence of SEQ ID NO: 2 or SEQ ID NO: 3, except that said polypeptide contains a substitution of at least: (i) one of the residues in positions 4, 20, 49, 52, 92, 94 and/or 95 of SEQ ID NO: 2 or SEQ ID NO: 3, and/or (ii) one of the residues in positions 101, 102, and/or 109 of SEQ ID NO: 2 or SEQ ID NO: 3, if present; wherein said polypeptide shows increased cleavage activity at 37° C. against a DNA target it recognizes and cleaves in comparison to the cleavage activity of an identical polypeptide which does not comprise the substitutions of the residues specified in (i) and (ii) above against said DNA target.