Patent ID: 7977084

Claim:
A non-naturally occurring microbial organism, comprising exogenous nucleic acids encoding a malonyl-CoA-independent fatty acid synthesis (FAS) pathway and exogenous nucleic acids encoding an acyl-reduction pathway expressed in sufficient amounts to produce a primary alcohol having 4-24 carbon atoms, said malonyl-CoA-independent FAS pathway comprising exogenous nucleic acids encoding ketoacyl-CoA acyltransferase or ketoacyl-CoA thiolase, 3-hydroxyacyl-CoA dehydrogenase, enoyl-CoA hydratase and enoyl-CoA reductase, said acyl-reduction pathway comprising one or more exogenous nucleic acids encoding an acyl-CoA reductase and an alcohol dehydrogenase, wherein said exogenous nucleic acids encoding said malonyl-CoA-independent FAS pathway and said exogenous nucleic acids encoding said acyl-reduction pathway are expressed in the cytosol, wherein said ketoacyl-CoA acyltransferase or ketoacyl-CoA thiolase converts acyl-CoA to β-ketoacyl-CoA, wherein said 3-hydroxyacyl-CoA dehydrogenase converts β-ketoacyl-CoA to β-hydroxyacyl-CoA, wherein said enoyl-CoA hydratase converts β-hydroxyacyl-CoA to trans-2-enoyl-CoA, wherein said enoyl-CoA reductase converts trans-2-enoyl-CoA to acyl-CoA, wherein said acyl-CoA reductase converts acyl-CoA to an aldehyde, wherein said alcohol dehydrogenase converts an aldehyde to said primary alcohol.