Patent ID: 7897838

Claim:
A method for producing a transgenic cotton plant, said method comprising: (a) providing a proembryogenic cotton cell; (b) culturing the proembryogenic cell in a liquid medium to produce a cell suspension culture; (c) providing a culture of preinduced Agrobacterium tumefaciens that harbors a vector comprising an exogenous gene and a selectable marker, the Agrobacterium being capable of effecting the stable transfer of the exogenous gene and selectable marker to the genome of a plant cell, wherein the preinduced Agrobacterium tumefaciens is preinduced for virulence; (d) mixing a portion of the cell suspension culture and a portion of the Agrobacterium culture; (e) co-culturing the mixture of the cell suspension culture and the Agrobacterium culture on a porous solid support in contact with a co-culture medium for 1-5 days under continuous light to produce a population of cells some of which have been transformed with the exogenous gene and the selectable marker; (f) selecting cells that express the exogenous gene from the population of cells; and (g) transferring the selected cells onto at least one regeneration medium comprising zeatin, wherein said cells regenerate into a transgenic cotton plant by somatic embryogenesis.