Patent ID: 8313955

Claim:
A test device for detecting the presence or absence of an analyte in a liquid sample comprising: a) a mobilization zone comprising a mobilizeable ligand and a mobilizeable antibody, the mobilizable antibody being capable of specifically binding the analyte and the mobilizeable ligand non-simultaneously, wherein the mobilizeable antibody and the analyte exhibit a binding affinity greater than that of the mobilizeable antibody and the mobilizeable ligand, and wherein the mobilizeable antibody is contacted by the sample before the mobilizeable ligand is contacted by the sample; b) a blocking zone located downstream from the mobilization zone, wherein the blocking zone comprises an immobilized trapping receptor that binds the mobilizeable ligand; and c) a detection zone located downstream from the blocking zone, wherein the detection zone comprises an immobilized detecting receptor that binds the mobilizeable antibody.