Patent ID: 8865237

Claim:
A method of preparing a dialyzed aqueous extract of fenugreek seeds comprising: (a) washing fenugreek seeds in distilled water; (b) sterilizing the seeds to form sterilized seeds; (c) grinding the sterilized seeds to form a powder; (d) suspending the powder in phosphate buffered saline (PBS) comprising phenylmethylsulfonylflouride (PMSF) and a protease inhibitor cocktail to provide a suspension; (e) filtering the suspension to obtain a filtrate; (f) treating the filtrate with activated charcoal to obtain a clear supernatant; (g) subjecting the clear supernatant to a step of lyophilization to obtain a lyophilized powder (h) dissolving the lyophilized powder in phosphate buffered saline (PBS) to obtain a dissolved extract; and (i) dialyzing and storing the dissolved extract to obtain the dialyzed aqueous extract of fenugreek seeds.