Patent ID: 8633297

Claim:
A recombinant polypeptide scaffold comprising: I. seven beta strand domains designated A, B, C, D, E, F, and G, having the amino acids of: a) SEQ ID NO:239 for the A beta strand, SEQ ID NO:229 for the B beta strand, SEQ ID NO:230 for the C beta strand, SEQ ID NO: 236 for the D beta strand, SEQ ID NO:232 for the E beta strand, SEQ ID NO:245 for the F beta strand and SEQ ID NO:246 for the G beta strand; or b) SEQ ID NO:239 for the A beta strand, SEQ ID NO:229 for the B beta strand, SEQ ID NO:247 for the C beta strand, SEQ ID NO: 236 for the D beta strand, SEQ ID NO:232 for the E beta strand, SEQ ID NO:248 for the F beta strand, and SEQ ID NO:234 for the G beta strand; II. linked to six loop regions such that a loop region connects each beta strand domain, is designated AB, BC, CD, DE, EF, and FG and corresponds to the cognate loop regions of SEQ ID NO:1, the cognate loop regions of SEQ ID NO: 1 consisting of residues: a) from 11 to 17 inclusive in an AB loop; b) from 23 to 31 inclusive in an BC loop; c) from 39 to 45 inclusive in an CD loop; d) from 51 to 56 inclusive in an DE loop; e) from 23 to 31 inclusive in an EF loop; f) from 23 to 31 inclusive in an FG loop, at least one of said loop regions being a non-naturally occurring variant of the cognate loop region in SEQ ID NO: 1; and III. a disulfide bond linking the F and G beta strand domains or linking the C and F beta strand domains, wherein said scaffold binds a target.