Patent ID: 7141401

Claim:
A method for selecting modulators of an interaction between a first protein and a second protein, (a) said first protein being selected from the group consisting of (i) cyclooxygenase 1 (COX1) (SEQ ID NO:1), (ii) a homologue of COX1 having an amino acid sequence at least 90% identical to that of COX1 of (i), or fragments thereof, and capable of interacting with thyroid hormone responsive Spot 14 (THR S14) (SEQ ID NO:2) or protein KIAA0567 (Opa1) (SEQ ID NO:3), (iii) a fragment of COX1 of (i) capable of interacting with THR S14 of (ii) or Opa1 of (ii), and (iv) a fusion protein containing COX1 of (i), said homologue of COX1 of (ii), or a fragment thereof, or said COX1 fragment of (iii), and capable of interacting with THR S14 of (ii) or Opa1 of (ii); and (b) said second protein being selected from the group consisting of (1) thyroid hormone responsive Spot 14 (THR S14) (SEQ ID NO:2) or protein KIAA0567 (Opa1) (SEQ ID NO:3), (2) a homologue of THR S14 or Opa1 having an amino acid sequence at least 90% identical to that of THR S14 of (1) or Opa1 of (1), or a fragments thereof, and capable of interacting with COX1 of (i), (3) a fragment of THR S14 of (1) or Opa1 of (1) capable of interacting with COX1 of (i), and (4) a fusion protein containing THR S14 of (1) or Opa1 of (1), said homologue of THR S14 of (2) or Opa1 of (2), or fragments thereof, or said fragment of THR S14 of (3) or Opa1 (3), capable of interacting with COX1 of (i), said method comprising: contacting said first protein with said second protein in the presence and absence of a test compound; and detecting a difference in the interaction between said first protein and said second protein in the presence and absence of the test compound.