Patent ID: 8431341

Claim:
A method of identifying an inhibitor of tRNA-target molecule interaction, comprising: forming a mixture comprising a first nucleic acid molecule comprising a tRNA D-loop fragment, a target molecule capable of binding to the tRNA D-loop fragment, and a test compound, wherein the tRNA D-loop fragment contains at least one modified nucleotide; incubating the mixture under conditions that allow binding of the tRNA D-loop fragment and the target molecule in the absence of the test compound; and detecting whether or not the test compound inhibits the binding of the tRNA D-loop fragment and the target molecule, wherein the absence of binding of the tRNA fragment and the target molecule is indicative of the test compound being an inhibitor of tRNA-target molecule interaction, wherein the mixture further comprises a second nucleic acid molecule comprising an anticodon stem-loop fragment, wherein the target molecule is capable of binding to the tRNA D-loop fragment and the ASL fragment, and wherein the anticodon stem-loop contains at least one modified nucleotide.