Patent ID: 8822415

Claim:
A dilute cytokine-containing solution composition made by a method comprising the step of a) combining i) Amnion-derived Cellular Cytokine Solution (ACCS), wherein the ACCS comprises about 5.0-16 ng/mL VEGF, about 3.5-4.5 ng/mL Angiogenin, about 100-165 pg/mL PDGF, about 2.5-2.7 ng/mL TGFβ2, about 0.68 μg/mL TIMP-1 and about 1.04 μg/mL TIMP-2; and ii) a diluent, such that the dilute cytokine-containing solution composition has a final concentration of less than 5 ng/mL of VEGF, less than 2.5 ng/mL of TGFβ2, less than 3.5 ng/mL of Angiogenin, less than 100 pg/mL of PDGF, less than 0.68 μg/mL of TIMP-1, and less than 1.04 μg/mL of TIMP-2.