Patent ID: 8603835

Claim:
A test device for determining the presence of a ligand in a sample, comprising: a) buffer solution; b) a first sorbent strip having a first location for receiving a sample and defining a first migration path; c) a second sorbent strip distinct from said first sorbent strip, said second sorbent strip having a second location for receiving said buffer solution and at least partially defining a second migration path distinct from said first migration path and elongated relative to said first migration path; d) a conjugate supported by said second sorbent strip; e) a test site located on or in at least one of said first sorbent strip and said second sorbent strip, said test site having an immobilized ligand-binding mechanism, and said first and second sorbent strips touching each other at the test site; f) a frangible container and divider located at said second location, said frangible container containing said buffer solution, said divider directing a first amount of said buffer solution to said first sorbent strip to move said sample to said test site and a second amount to said second sorbent strip to move said conjugate to said test site upon breaking of said frangible container; and g) a housing defining a first opening adjacent said first location, a second opening adjacent said second location, and a window adjacent said test site through which said test site is viewable, wherein said first migration path is provided with a first length and said second migration path is provided with a second length, and said first length said second length are chosen to permit said sample to reach said test site so that ligand in said sample reaches said test site and binds to said immobilized ligand-binding mechanism prior to said conjugate reaching said test site.