Patent ID: 8131479

Claim:
A method for detecting plural types of phosphorylated proteins contained in a phosphorylated protein detection target sample comprising the steps of: (a) analyzing masses of plural types of proteins contained in a sample which is the same type of sample as said phosphorylated protein detection target sample; (b) identifying proteins by referring the mass data obtained from the analysis in step (a) to an existing 1st protein database; (c) newly creating a 2nd database which only contains the data of the proteins identified in step (b) and key information and protein names used for search in addition to amino acid sequence information of the proteins identified in step (b); (d) separating plural types of phosphorylated proteins from the phosphorylated protein detection target sample; (e) analyzing masses of the phosphorylated proteins separated in step (d); and (f) retrieving the mass data obtained in step (e) from the 2nd database created in step (c), thereby detecting the phosphorylated proteins.