Patent ID: 8426133

Claim:
A method for detecting a dysregulation in a target gene comprising: (a) amplifying a 5′ region of the target gene transcript, if present, in a nucleic acid sample with one or more 5′ target primer pairs which are complementary to the 5′ region of the target gene; (b) amplifying a 3′ region of the target gene transcript, if present, in the biological sample with one or more 3′ target primer pairs which are complementary to the 3′ region of the target gene; (c) detecting the amounts of amplification product produced by the one or more 5′ target primer pairs and the one or more 3′ target primer pairs; (d) comparing the relative expression of the 5′ region to the 3′ region of the target gene in the nucleic acid sample to the relative expression of the 5′ region to the 3′ region of the target gene in a reference sample; and (e) identifying the nucleic acid sample as having a gene dysregulation when the amounts of amplification products produced by steps (a) and (b) indicates that the target gene is dysregulated.