Patent ID: 8852585

Claim:
A method for preparing an immunoglobulin preparation comprising immunoglobulin M (IgM) molecules that specifically bind Aβ1-42 peptides from pooled human plasma comprising: (a) performing a first caprylate precipitation such that a first precipitate and a first supernatant comprising immunoglobulins are formed; (b) separating the first supernatant from the first precipitate; (c) performing a second caprylate precipitation such that a second precipitate and a second supernatant comprising immunoglobulins are formed; (d) separating the second supernatant from the second precipitate; (e) contacting the second supernatant with a first anion exchange resin; (f) separating a fraction containing substantially all of the immunoglobulin G and immunoglobulin M from the result of step (e); (g) contacting the fraction of step (f) with a second anion exchange resin; (h) eluting IgM from the second anion exchange resin column; (i) applying the IgM to a gel filtration resin and recovering the IgM; (j) applying the IgM to an affinity resin comprising immobilized antigens A and B; and (k) recovering the eluted IgM to obtain a purified, virally inactivated IgM preparation.