Patent ID: 8409874

Claim:
A method for the selective disassociation of at least one bound glycosaminoglycan from a plasma polymerized surface of an organic monomer comprising an allylamine, said method comprising in consecutive order: providing a plasma polymerized surface comprising said allylamine; coating said plasma polymerized surface with a solution comprising phosphate buffered saline and at least one glycosaminoglycan; incubating said coated plasma polymerized surface to induce binding of said at least one glycosaminoglycan; contacting said surface with at least one agent having a salt concentration of about 500 mM NaCl to about 2 M NaCl, wherein said agent provides for selective disassociation of said at least one bound glycosaminoglycan from said plasma polymerized surface; washing said incubated coated plasma polymerized surface; incubating said washed plasma polymerized surface with a solution comprising tumor necrosis factor-inducible gene 6 protein (TSG-6); and detecting an amount of TSG-6 bound to said plasma polymerized surface, wherein said amount is inversely indicative of the selective disassociation of said at least one bound glycosaminoglycan from said plasma polymerized surface.