Patent ID: 8545874

Claim:
A method for making an NLP (Nanolipidic Particle) assembly population, comprising the steps of: (a) diluting a monophasic optically clear precursor solution with an ethanol solvent to form solvent diluted stock solution; wherein said diluting of said precursor solution with said ethanol solvent is at a ratio ranging from about 1 part precursor to about 20 parts solvent to a ratio ranging from about 1 part precursor to about 0.3 parts solvent; and (b) adding an aqueous solution comprising a water soluble passenger molecule to said solvent diluted stock solution, whereby an NLP assembly population is formed having a mean particle diameter from about 30 to 200 nm; and wherein the concentration of said solvent in said NLPs is from about 0.5% to about 14%.