Patent ID: 8608661

Claim:
A method comprising: positioning a delivery device at a location in a blood vessel, the delivery device comprising a balloon; positioning an imaging device disposed within a lumen of the delivery device at the location, the lumen dimensioned to be shared simultaneously or sequentially with a guidewire and comprising a plug member to divide the lumen into a first region and a second region distal to the first region, the imaging device is positioned within the first region and the second region is operable to accommodate a guidewire; imaging with the positioned imaging device and measuring a thickness of a portion of a wall of the blood vessel at the location; identifying a treatment site beyond an external elastic lamina of the blood vessel based on the imaging and measuring; after identifying the treatment site, inflating the balloon; after inflating the balloon, advancing a needle from a delivery cannula coupled to an exterior surface of the balloon a distance into the wall of the blood vessel to the identified treatment site beyond an external elastic lamina of the blood vessel; and after advancing the needle, introducing a treatment agent comprising a cellular component from the needle.