Patent ID: 7785884

Claim:
A method of producing conifer cotyledonary somatic embryos, the method comprising: (a) uniformly dispersing pre-cotyledonary somatic embryos onto a porous material horizontally disposed over a non-porous surface at a density of less than 0.05 gram wet cell weight of pre-cotyledonary somatic embryos per square inch of porous material by suspending the pre-cotyledonary embryos in a volume of sterile dilution medium sufficient to submerge the surface of the porous material and dispensing the pre-cotyledonary somatic embryos in the sterile dilution medium onto the porous material; (b) removing the sterile dilution medium from the porous material, thereby trapping the uniformly dispersed pre-cotyledonary somatic embryos on the porous material; and (c) contacting the pre-cotyledonary somatic embryos trapped on the porous material with development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos.