Patent ID: 8053187

Claim:
A method of providing complementary nucleic acid comprising: a) providing oligonucleotides, wherein said oligonucleotides are generated according to the method of: A) providing: i) a substrate comprising an array of oligonucleotides attached to said substrate via anchor moieties attached to non-cleavable linkers attached to said substrate, wherein said anchor moieties comprise the structure —C(X)—C(Y), wherein X comprises —OPO 2 O—, Y is a nucleophile and wherein said structure is part of a ring moiety; ii) a deprotecting solution; and iii) and a wash solution; B) deprotecting said oligonucleotides with said deprotecting solution; C) washing said oligonucleotides with said wash solution; and D) cleaving said oligonucleotides at said anchor moieties to provide purified oligonucleotides; b) mixing said oligonucleotides with a polymerase and template strands having a complementary sequence to the oligonucleotide in a polymerization buffer; and c) recovering copies of complementary nucleic acid strands in the duplex with the template strand.