Patent ID: 8315681

Claim:
A method comprising the steps of: inducing a change in glucose metabolism in a nutrient capillary in skin by temperature-changed glycolysis; measuring a change in localized reflectance light signals at a plurality of light source-detector distances and a plurality of wavelengths over a specific time period after skin-probe contact as a function of a time for which a localized reflectance probe is brought into contact with the skin, said temperature-changed glycolysis causing a change with respect to light attenuation, oxygen consumption in a tissue and concentration of a hemoglobin variant; selecting a time window in which a tissue-probe adaptation effect on the signals is minimized and an effect on glycolysis induced by temperature has time dependence, and using a signal measured in the time window for a subsequent calculation; calculating one set of functions based on a plurality of localized reflectance values at the plurality of light source-detector distances and the plurality of wavelengths at a plurality of time intervals in the time window and at least two wavelengths; deriving a calibration relationship between a combination of the calculated functions and a glucose concentration in a living body; and using the calibration relationship for predicting a glucose concentration in a body fluid in subsequent measurement, wherein at least one function in the plurality of time intervals is associated with a change in oxygen consumption.