Patent ID: 7824901

Claim:
An apparatus for carrying out fusion of biological cells, comprising: an inner electrode having a first electrode radius (r 1 ) and an electrode height, an outer electrode having a second electrode radius (r 2 ) and said electrode height, wherein said inner electrode and said outer electrode are concentric, a gap between said inner electrode and said outer electrode, wherein the size of said gap is the difference between said second electrode radius and said first electrode radius, and wherein a cell fusion volume is defined by said electrode height, said gap, said first electrode radius (r 1 ), and said second electrode radius (r 2 ), wherein said first electrode radius, said second electrode radius, and said gap are selected in accordance with a predetermined range of selectable ratios of said first electrode radius to said second electrode radius, wherein said range of selectable ratios is from 0.7 to 0.9, a selected gap limited to a range from 2 to 10 millimeters, and a determined ratio of said selectable ratios based on said selected gap, such that compression between the biological cells and permeability between cell membranes are maximized and temperature rise is minimized for providing cell fusion in said cell fusion volume.