Patent ID: 7695943

Claim:
A process for the preparation of 1,3-propanediol from a carbon-containing substance comprising growing a recombinant micro-organism in the presence of said carbon-containing substance, wherein said recombinant micro-organism comprises at least one nucleic acid encoding for a bacterial 1,3-propanediol dehydrogenase and at least one nucleic acid encoding for a glycerol dehydratase, wherein: a) said glycerol dehydratase is a dimeric protein comprising:(a) a first polypeptide having at least 90% amino acid identity with the amino acid sequence of SEQ ID NO.6; and (b) a second polypeptide having at least 90% amino acid identity with the amino acid sequence of SEQ ID NO.7 and b) catalytic activity of said glycerol dehydratase is not dependent on coenzyme B12 or one of its precursors.