Patent ID: 8101425

Claim:
A method of manufacturing a device for filtering and separating at least one target agent from a sample, said device comprising one or more stacked layers of resin-embedded membranes comprising first and second layers of porous nonwoven fabric that are bonded together with a plurality of resin particles impregnated therein or sandwiched therebetween, said porous nonwoven fabric having pore sizes greater than 10 μm, and the resin particles having a size of 40-200 μm, wherein the at least one target agent attaches to the porous nonwoven fabric, the resin particles or both, and is removed from the sample, which method comprises the steps of: (a) spreading the plurality of resin particles on a first layer of said porous nonwoven fabric, (b) placing a second layer of said porous nonwoven fabric over the first layer to form a double layer, and (c) calendering the double layer to form a resin-embedded membrane.