Patent ID: 7932070

Claim:
An enzyme mixture comprising a first enzyme and a second enzyme, wherein said first enzyme comprises 5′-3′ polymerization activity of a DNA polymerase or reverse transcriptase, and said second enzyme is a mutant of a wild type DNA polymerase, said wild type DNA polymerase comprising the partitioning domain sequence YXGG (SEQ ID NO:6), the polymerase domain sequence DXXSLYP (SEQ ID NO:1) or DFRALYP (SEQ ID NO:13), the polymerase domain sequence YXDTDS (SEQ ID NO:4), YIDTDG (SEQ ID NO:15), YADTDG (SEQ ID NO:16), or YSDTDG (SEQ ID NO:17), and the polymerase domain sequence KXY, wherein said second enzyme comprises an amino acid substitution at an amino acid position corresponding to G387 of SEQ ID NO:19, and wherein said second enzyme comprises 3′-5′ exonuclease activity and reduced 5′-3′ DNA polymerization activity as compared to the wild type DNA polymerase.