Patent ID: 8445413

Claim:
A linker for ligating mRNA and puromycin or a puromycin-like compound to construct an mRNA-puromycin conjugate, an mRNA-puromycin-protein conjugate or an mRNA/cDNA-puromycin-protein conjugate, the linker comprising the nucleic acid sequence of SEQ ID NO:1 as a main backbone, wherein a solid phase binding site consisting of biotin-deoxythymine is at position 5, a pair of cleavage sites consisting of Ribo-guanosines and cleavable by an enzyme are at positions 3 and 7, and a puromycin or puromycin-like compound conjugating site consisting of amino-modified deoxythymine is at position 23, and wherein the enzyme is selected from the group consisting of RNase T1, RNase A, RNase I and pancreatic RNase.