Patent ID: 8202517

Claim:
A method for delivering a therapeutically effective drug or pharmaceutical agent to a subject, comprising: a) administering a hyaluronidase glycoprotein to a tissue in the subject in an amount sufficient to degrade glycosaminoglycans, wherein: the hyaluronidase glycoprotein consists of the sequence of amino acids set forth as amino acids 36-477, 36-478, 36-479, 36-480, 36-481, 36-482, or 36-483 of SEQ ID NO: 1, or contains amino acid substitutions in the sequence of amino acids set forth as amino acids 36-477, 36-478, 36-479, 36-480, 36-481, 36-482 or 36-483 of SEQ ID NO:1, whereby the amino-acid substituted hyaluronidase glycoprotein consists of a sequence of amino acids that has at least 95% amino acid sequence identity with the sequence of amino acids set forth as amino acids 36-477, 36-478, 36-479, 36-480, 36-481, 36-482 or 36-483 of SEQ ID NO:1; the glycoprotein is neutral active; and the glycoprotein contains at least one sugar moiety that is covalently attached to an asparagine (N) residue of the polypeptide; and b) administering a therapeutically effective amount of the drug or pharmaceutical agent to the subject.