Patent ID: 7846458

Claim:
A method for treatment of an infection caused by Pythium insidiosum in human patients which comprises: (a) providing a vaccine containing a mixture of (1) mixed intracellular proteins and (2) mixed extracellular proteins of Pythium insidiosum in a sterile aqueous solution, wherein the mixed intracellular proteins, which consist essentially of the intracellular proteins removed as a supernatant separated from disrupted cells of the Pythium insidiosum grown in a culture medium, and the mixed extracellular proteins, which consist essentially of proteins removed from the culture medium for growing the Pythium insidiosum the mixed intracellular proteins and the mixed extracellular proteins have by been precipitated together with acetone, separated and then mixed with water and the mixture has been dialyzed to remove low molecular weight components less than 10,000 MW; and (b) vaccinating the human the patient with the vaccine.