Patent ID: 7736845

Claim:
A method for processing collagenous connective tissue having a structure of collagen fibers from an animal donor source such that upon implant of said tissue into a recipient said tissue is acceptable to the recipient without an immune and inflammatory rejection, comprising the steps of: (a) stabilizing the collagen fibers contained in said collagenous connective tissue in a cold stabilizing solution for a period of time and at a temperature sufficient to retain the structure of the collagen fibers where said temperature range is 4 to 10 degrees centigrade; (b) soaking said collagenous connective tissue in a first solution comprising predetermined quantities and concentrations of polyglycol, an oxidizing agent, salt and phosphate buffer at a predetermined temperature where said first solution is of sufficient ionic strength to permit ground substances to dissociate from said collagenous connective tissue such that the collagen fibers remain stable; (c) after step (b), washing said collagenous connective tissue in predetermined quantities and concentrations of a second solution comprising alcohol and water for a sufficient period of time at a predetermined temperature to sufficiently remove the residue of said first solution therefrom; (d) soaking said collagenous connective tissue in a third solution comprising a predetermined quantity and concentration of an anti-inflammatory agent at a predetermined temperature; and (e) soaking said collagenous connective tissue in a fourth solution comprising a predetermined quantity and concentration of an anti-thrombic agent at a predetermined temperature.