Patent ID: 8153441

Claim:
A method for analyzing fragmented structures of [ 123 I]ADAM, comprising: providing a high-performance liquid chromatography tandem mass spectrometer (HPLC-MS/MS); providing [ 127 I]ADAM, dissolving [ 127 I]ADAM in methanol, and ionizing the parent molecule [ 127 I]ADAM into its parent ion; performing a Q1 scan on the parent ion by using a mass spectrometer in the HPLC-MS/MS, and fragmenting the parent ion into product ions to find optimum parameters of declustering potential (DP), entrance potential (EP), collision energy (CE), and collision cell exit potential (CXP) when the following m/z of the MRM transition product ions are obtained: m/z 385.0→m/z 340.0, m/z 385.0→m/z 212.5, m/z 385.0→m/z 196.5, m/z 385.0→m/z 184.5, m/z 385.0→m/z 180.5, m/z 385.0→m/z 165.6, m/z 385.0→m/z 152.5; providing the HPLC-MS/MS and setting: eluent, methanol/acetonitrile/ammonium acetate solution (10 mM, pH 7.0), 79.68:19.92:0.40 (v/v/v); and flow rate, 0.4 mL/min; performing a multiple reaction monitoring (MRM) chromatography on [ 127 I]ADAM by using the HPLC-MS/MS with the parameters of DP, EP, CE, and CXP found above, to obtain mass charge ratios (m/z) of MRM transition product ions; deducing a fragmentation pathway of the parent molecule [ 127 I]ADAM from the m/z transitions of the MRM transition product ions.