Patent ID: 8241673

Claim:
A method for obtaining bone plates, from tissue of a bone, the method comprising: (a) cleaning the bone; (b) sawing the bone into plates having a thickness of 0.2-2.0 cm; (c) washing the plates twice in a 0.1 M phosphate buffer solution having a pH of 5.8-6.0 and a temperature of 65° C., wherein the ratio of bone plates to buffer solution is 1:2 by volume; (d) immersing the plates in a 0.1-0.4% solution of activated papain at 65° C. for 24 hours; (e) washing the plates five times with water having a temperature 40-80° C.; (f) treating the plates with 0.4 N alkali solution at room temperature for 10-24 hours; (g) washing the plates with water; (h) drying the plates; (i) removing fat from the plates by washing with an ethanol and chloroform mixture, wherein the ratio of ethanol to chloroform is 2:1 by volume; (j) removing fat from the plates by washing with an ethanol and chloroform mixture, wherein the ratio of ethanol to chloroform is 1:2 by volume; (k) decalcifying the plates with a solution of hydrochloric acid; (l) treating the plates with a solution of hydrogen peroxide for 4 hours; (m) washing the plates with water at room temperature until the hydrogen peroxide is removed from the plates; (n) washing the plates with ethanol; (o) drying the plates; (p) packing the plates; and (q) sterilizing the plates.