Patent ID: 7947435

Claim:
A method for screening for a neuronal regeneration material comprising the following steps: 1) culturing, on a filter, a single tissue slice obtained from the lumbar spinal cord of a mammal in a well, wherein the thickness of the tissue slice is 400 μm; 2) damaging the tissue slice in the course of culturing the single tissue slice of step 1) by treating with a toxic compound, which induces demyelination of axons in the spinal cord; 3) contacting neuronal regeneration material candidates to the damaged tissue slice; and 4) selecting a neuronal regeneration material candidate that induces significant directional neuronal regeneration, from the tissue slice to the neuronal regeneration material candidate, compared with the negative control untreated with the neuronal regeneration material candidates; wherein a spinal cord slice treated with mesenchymal stem cells is used as a positive control and the negative control group is a spinal cord slice untreated with the mesenchymal stem cells.