Patent ID: 8790895

Claim:
A method for the preparation of a modified ubiquitin polypeptide comprising a ubiquitin-like folding motif that has a binding affinity to a predetermined binding partner, the method comprising: a) providing one or more modified ubiquitin polypeptides comprising beta strands, wherein at least one of the one or more modified ubiquitin polypeptides comprises substitutions of four to eight amino acids at positions corresponding to Gln2, Phe4, Lys6, Gln62, Lys63, Glu64, Ser65 and Thr66 of SEQ ID NO: 35 or of the amino acid sequence encoded by SEQ ID NO: 15; b) predetermining a binding partner, wherein the predetermined binding partner exhibits a binding affinity with respect to the modified ubiquitin that did not exist previously with respect to a ubiquitin polypeptide comprising amino acid sequence SEQ ID NO: 35; c) contacting the one or more modified ubiquitin polypeptides with the binding partner predetermined in step b); and d) identifying a modified ubiquitin polypeptide having a dissociation constant (K D ) of from 10 −5 M to 10 −12 M with respect to the binding partner predetermined in step b).