Patent ID: 8101414

Claim:
A reagent kit for measuring a basophil and/or a nucleated red blood cell, comprising: a first solution containing an alcohol having 4 to 8 total carbon atoms, the concentration of the alcohol in the first solution being 0.01 to 10% by weight and a surfactant for hemolyzing a red blood cell and giving damage to a cell membrane of a leukocyte to such an extent that a fluorescent dye is capable of permeating therethrough, and a second solution containing one or more fluorescent dyes selected from the group consisting of a compound of the general formula (I): (wherein R 1 and R 2 are independently an alkyl group, R 3 , R 4 , R 5 and R 6 are independently a hydrogen atom or an alkyl group, and X − is an anion), and a compound of the general formula (II): (wherein R 7 and R 8 are independently an alkyl group, wherein the alkyl group may have an acidic group which may form a salt: R 9 , R 10 , R 11 and R 12 are independently a hydrogen atom or an acidic group which may form a salt, and at least one of R 7 to R 12 has an acidic group which has released a proton).