Patent ID: 7893228

Claim:
A method for purifying RNA from biological material comprising RNA, comprising: (a) mixing said biological material with an RNA Lysing Solution, wherein the RNA Lysing Solution (i) is buffered at a pH of greater than 7, (ii) comprises an amphiphillic reagent and an RNA complexing salt, wherein the RNA-complexing salt is an alkali-metal salt present at a concentration greater than 4 M, and (iii) is free of a strong chaotropic substance; (b) lysing said biological material with said RNA Lysing Solution to form a lysate comprising RNA and non-nucleic acid biological matter; (c) contacting said lysate to an immobilized non-silica solid support, wherein said RNA in said lysate preferentially binds to said solid support; (d) washing said solid support with an RNA wash solution to remove non-nucleic acid biological matter; and (e) preferentially eluting the bound RNA from said solid support with an RNA elution solution to obtain the RNA.