Patent ID: 8148094

Claim:
A method of detecting the activation state of single cells, said method comprising the steps of: a) providing a cell comprising a first and a second activatable protein, wherein said first and second activatable proteins are different proteins that each have at least an activated isoform, and a non-activated isoform; b) contacting said cell with at least two activation state-specific antibodies, wherein a first of said at least two activation state-specific antibodies is specific for said activated isoform of said first activatable protein; and a second of said at least two activation state-specific antibodies is specific for said activated isoform of said second activatable protein, wherein said two activation state-specific antibodies are distinguishably detectable; and c) detecting a presence or absence of binding of said first and said second activation state-specific antibodies to their corresponding activated isoform of said first and second activatable proteins in said cell, wherein said presence or absence of binding of said first activation state-specific antibody is indicative of the activation state of said first activatable protein, and said presence or absence of binding of said second activation state-specific antibody is indicative of the activation state of said second activatable protein.