Patent ID: 8679813

Claim:
An isolated variant of a parent lipase, wherein the parent lipase comprises the amino acid sequence of the positions 1 to 269 of the mature polypeptide shown in SEQ ID NO: 2 or an amino acid sequence having at least 90% amino acid sequence identity with the amino acid sequence of the positions 1 to 269 of the mature polypeptide shown in SEQ ID NO: 2 and having a lipase activity, and the variant lipase comprises one of the following modifications: (1) I202G+T231R+N233R; (2) I86V+L227G+T231R+N233R+P256K; (3) Q4V+S58N+V60S+T231R+N233R; (4) S58N+V60S+190R+T231R+N233R; (5) I255Y+T231R+N233R; and (6) I90A+T231R+N233R+I255V; and wherein the variant lipase has lipase activity, and further has an Average Relative Performance (RPave) of at least 0.8 and a Benefit Risk (BR) of at least 1.1 in comparison with those of the Reference lipase consisting of the amino acid sequence of the positions 1 to 269 of the mature polypeptide shown in SEQ ID NO: 2 which contains the modification T231R+N233R.