Patent ID: 8648016

Claim:
A method of quantitating the concentration of a molecule of interest comprising: identifying, for a first and a second test site, a respective capturing agent recipe, wherein the capturing agent recipe for each of the first and the second test sites is different from the capturing agent recipe for the other of the first and the second test sites; immobilizing, in each of the first and the second test sites, a first capturing agent in accordance with the identified recipe; establishing a first test environment at the first test site; establishing a second test environment at the second test site such that a response curve for the second site is different from a response curve of the first site; storing a combined response curve resulting from a summation of the response curve for the second site and the response curve of the first site; exposing the first test site and the second test site to a sample having a concentration of a molecule of interest; obtaining a first quantitation signal from the first test site; obtaining a second quantitation signal from the second test site; combining the first quantitation signal and the second quantitation signal; associating the combined first quantitation signal and second quantitation signal with the stored response curve; and determining the concentration of the molecule of interest based upon the association.