Patent ID: 8029979

Claim:
A method for determining a substrate specificity of at least one enzyme which comprises the steps of: providing an assembly comprising a plurality of amino acid sequences on a planar, non-porous surface of a support material wherein the amino acid sequences are substantially directionally immobilized in a single direction at either the N-terminus only or the C-terminus only of the amino acid sequences to the surface by reacting a defined reactive group on the amino acid sequence with a defined reactive group on the surface, wherein all amino acid sequences are immobilized using the same terminus and the same reactive group resulting in similar secondary and tertiary structures, contacting and/or incubating of the enzyme with the assembly, detecting a reaction between one or a plurality of amino acid sequences immobilized on the assembly and the enzyme, wherein during said reaction, a change in molecular weight of at least one of the amino acid sequences takes place, and determining substrate specificity of the enzyme based on an arrangement of said amino acid sequences at distinct locations.