Patent ID: 7030230

Claim:
A process of purifying a phosphitylated compound of formula I: wherein G 1 is O or S; G 2 is a protecting group; G 3 is an amine; Z is Z 1 or Z 2 ; Z 1 has the formula: Z 2 has the formula: Y is O or S; R′ 2 is protected OH,; H, HO, OR 5 , NR 5 R 6 , F, Cl, Br, alkyl, substituted alkyl, heterocyclyoalkyl, substituted heterocycloalkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, aralkyl, substituted aralkyl, heterocycloaralkyl, substituted heterocycloaralkyl; or R′ 2 and R′ 4 together form a bridge; each of R 5 and R 6 is independently alkyl, heterocycloalkyl, substituted heterocycloalkyl, aryl, substituted aryl, aralkyl, substituted aralkyl, hetaryl, substituted hetaryl, hetarylalkyl, substituted hetarylalkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, alkanoyl, substituted alkanoyl, trialkylsilyl or substituted trialkylsilyl; or when R 5 and R 6 are on the same nitrogen, R 5 and R 6 may be taken together with the nitrogen to which they are bound to form a heterocyclyl ring or a substituted heterocyclyl ring R′ 4 is H or together with R′ 2 forms a bridge; m is an integer of 0 or 1, B x is a heterocycle and G 4 is a hydroxy protecting group; the process comprising: (a) providing the compound of formula I in a polar phase comprising a polar organic solvent and at least one impurity; (b) adding a basic compound and a first portion of water to the polar phase; (c) contacting the polar phase with a first apolar organic phase; (d) separating the first apolar organic phase from the polar phase; (e) adding a second portion of water to the polar phase and contacting the polar phase with a second apolar organic phase, the polar phase and the second apolar phase being contacted for a time sufficient for the compound of formula I to partition into the second apolar organic phase; and (f) separating the second apolar phase from the polar phase.