Patent ID: 7575902

Claim:
A method of sequencing at least one target nucleic acid template comprising: (a) forming at least one reaction composition comprising at least one target nucleic acid template, at least one primer, at least one polymerase, at least one universal nucleotide, and at least one specific terminator, wherein the at least one universal nucleotide has a structure wherein: R 2 , R 3 , and R 4 are each independently selected from hydrogen, halogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, alkoxy, aryloxy, and alkylaryl; and R 5 is selected from hydrogen and —OH; (b) incubating the reaction composition under appropriate conditions to generate at least one primer extension product comprising one or more of the at least one universal nucleotides and a specific terminator; (c) separating one or more of the at least one primer extension products, wherein the separating comprises at least one mobility-dependent analysis technique; and (d) detecting one or more of the at least one primer extension products.