Patent ID: 7205126

Claim:
A method for treating a patient with a papillomavirus pseudovirus including plasmids, said method comprising: disrupting said papillomavirus-VLPs by mixing said papillomavirus-VLPs with a disruption buffer including a chelating agent; incubating the mixture of said disruption buffer and said papillomavirus-VLPs; mixing said disrupted papillomavirus-VLPs with said plasmids; adding a stop buffer to reassemble said papillomavirus-VLPs and said plasmids into pseudoviruses of VLPs with said plasmids inside; and incubating the mixture containing said pseudoviruses of VLPs; providing said papillomavirus pseudovirus including papillomavirus-VLPs with said plasmids inside; and directing that said pseudovirus be administered via the mucosa of said patient to induce a mucosal response.