Patent ID: 7812144

Claim:
A kit for use in the detection of mRNA transcripts from the E6 gene(s) of HPV, the kit containing primer-pairs which enable amplification of a region of E6 mRNA transcripts of HPV types 16, 18, 31, and 33, characterised in that the kit contains separate primer-pairs specific for E6 mRNA of each of the HPV types 16, 18, 31 and 33, the kit comprising one or more of, two or more of, or all of the following primer pairs and accompanying identification probes: (SEQ ID NO: 175) 5′ gatgcaaggtcgcatatgagCCACAGGAGCGACCCAGAAA and (SEQ ID NO: 177) 5′ AATTCTAATACGACTCACTATAGGGAGAAGGATTCCCATCTCTATAT ACTA with probe (SEQ ID NO: 19) TATGACTTTGCTTTTCGGGA or probe (SEQ ID NO: 187) ccagctTATGACTTTGCTTTTCGGGAagctgg; (SEQ ID NO: 196) 5′ gatgcaaggtcgcatatgagGAAAACGATGAAATAGATGGAG and (SEQ ID NO: 203) 5′ AATTCTAATACGACTCACTATAGGGAGAAGGGGTCGTCTGCTGAGCT TTCT with probe (SEQ ID NO: 32) GAACCACAACGTCACACAATG or probe (SEQ ID NO: 198) cgcatgGAACCACAACGTCACACAATGcatgcg; (SEQ ID NO: 210) 5′ gatgcaaggtcgcatatgagACTGACCTCCACTGTTATGA and (SEQ ID NO: 211) 5′ AATTCTAATACGACTCACTATAGGGAGAAGGTATCTACTTGTGTGCT CTGT with probe (SEQ ID NO: 50) GGACAAGCAGAACCGGACACATCCAA or probe (SEQ ID NO: 215) ccgtcgGGACAAGCAGAACCGGACACATCCAAcgacgg; and (SEQ ID NO: 221) 5′ GATGCAAGGTCGCATATGAGTATCCTGAACCAACTGACCTAT and (SEQ ID NO: 222) 5′ AATTCTAATACGACTCACTATAGGGAGAAGGTTGACACATAAACGA ACTG with probe (SEQ ID NO: 59) GGACAAGCACAACCAGCCACAGC or probe (SEQ ID NO: 225) ccaagcGGACAAGCACAACCAGCCACAGCgcttgg.