Patent ID: 8268609

Claim:
An yeast host cell capable of producing CMP-sialic acid comprising: (a) one or more nucleic acid molecules encoding a bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase, an N-acetylneuraminate-9-phosphate synthase, and a CMP-Sialic acid synthase, (b) a nucleic acid molecule encoding a CMP-sialic acid transporter, and (c) a nucleic acid molecule encoding a 2,6-sialyltransferase catalytic domain fused to a cellular targeting signal peptide encoded by nucleotides 1-108 of the S. cerevisiae Mnn2 wherein nucleotides 1-108 encoding the S. cerevisiae Mnn2 have the nucleotide sequence shown by nucleotides 13 to 120 of SEQ ID NO:103, wherein the yeast host cell is capable of producing the CMP-sialic acid.