Patent ID: 7622276

Claim:
A method for processing fixed biological tissue to remove phospholipids comprising the steps of: (a) immersing the fixed biological tissue in a first fresh bioburden reduction process (BRP) solution, wherein the phospholipids are soluble in the first BRP solution, and wherein the first BRP solution consists essentially of purified water, glutaraldehyde, polysorbate-80, and a buffer to maintain a pH within the range of about 7.3 to 7.5 during processing; (b) heating the first fresh BRP solution to a temperature of about 37° C. to remove phospholipids from the tissue; (c) removing the fixed biological tissue from the fresh BRP solution; (d) immediately immersing the fixed biological tissue in a next fresh BRP solution having a composition substantially the same as the first fresh BRP solution; and (e) heating the next fresh BRP solution to a temperature of about 37° C. to remove phospholipids from the tissue to mitigate or reduce calcification of the tissue.