Patent ID: 7390649

Claim:
A method of making a sensor chip comprising the steps of: (a) providing a carrier disposed in the sensor chip, and having a surface of metal oxide or metalloid oxide; and (b) homogenously layering a multiple layer of polysiloxane to the surface of the carrier, wherein the multiple layer of polysiloxane is obtained by a process comprising the steps of: i. immersing the carrier in a solution of a single bi-functional silane linker having a concentration in the range of from 0.1 to 50 weight % in an organic solvent, wherein a first functionality of the bi-functional silane linker comprising two or three hydrolysable atoms or groups on a silicon atom enables crosslinking, and wherein a second functionality of the bi-functional silane linker enables immobilization of sample or probe molecules; and ii. withdrawing the carrier at a speed in the range of from 0.1 to 10 mm/s, wherein bi-functional silane is fixed on the surface of the sensor chip by formation of a homogeneous multiple layer of polysiloxane through cross-linking, wherein the second functionality of the bi-functional silane linker is capable of undergoing nucleophilic substitution reactions, nucleophilic addition reactions, Diels-Alder reactions or radical substitution reactions.