Patent ID: 8481693

Claim:
A method of purifying Calicivirus virus-like particles (VLPs) using a multistep chromatographic process, wherein said VLPs are produced in insect cells, said method comprising (a) contacting a cell lysate or culture supernatant containing said VLPs with an ion exchange chromatographic material, wherein said VLPs bind to said ion exchange chromatographic material; (b) contacting the VLPs eluted from said ion exchange chromatographic material with a hydrophobic interaction chromatographic material, wherein said VLPs bind to said hydrophobic interaction chromatographic material; and (c) eluting said VLPs from said hydrophobic interaction chromatographic material, wherein said VLPs are purified to greater than about 70%, wherein the chromatographic materials used in the method do not comprise hydroxyapatite.