Patent ID: 8859246

Claim:
A method for producing poly 3-hydroxybutyrate-co-3-hydroxyvalerate by construction of a recombinant Escherichia coli carrying phaCAB of Ralstonia eutropha , wherein phaCAB is polyhydroxyalkanoates biosynthesis operon and propionyl-CoA synthase (prpE) genes of Salmonella enterica comprising the steps of: (a) Providing a DNA construct carrying phaCAB-vgb-prpE genes, wherein vgb is Vitreoscilla globin gene from Vitreoscilla stercoraria; (b) Providing an Escherichia coli strain; (c) Transforming the DNA construct into the Escherichia coli strain; (d) Screening and culturing the recombinant Escherichia coli; (e) Harvesting the recombinant Escherichia coli and producing poly 3-hydroxybutyrate-co-3-hydroxyvalerate by further purification; the prpE DNA fragment is ligated into the plasmid carrying phaCAB gene in cis, wherein the phaCAB gene encodes polyhydroxyvalerate synthase having the nucleotide sequence of SEQ ID NO: 1, and forms a DNA construct carrying phaCAB-vgb-prpE genes, which is subsequently transformed into the Escherichia coli ; following screening, the recombinant Escherichia coli is cultured in the medium for expression of poly 3-hydroxybutyrate-co-3-hydroxyvalerate.