Patent ID: 8765411

Claim:
A process for the production of human growth hormone (hGH) comprising: (i) fermenting human growth hormone producing E. coli cells; (ii) recovering inclusion bodies from the E. coli cells and dissolving the recovered inclusion bodies at an alkaline pH to provide dissolved hGH; (iii) optionally lyophilizing dissolved hGH; and carrying out the fermenting step in a culture medium comprising manganese, zinc, cobalt, molybdenum, calcium, copper, and boron as trace elements and wherein the culture medium comprises the trace elements in the following concentrations: Manganese 4.9 μM Zinc 8.0 μM Cobalt 7.0 μM Molybdenum 6.9 μM Calcium 16.9 μM Copper 6.1 μM Boron 6.7 μM based on the total volume of the culture medium.