Patent ID: 8221971

Claim:
A method of identifying an adenovirus serotype to administer as a recombinant replication defective adenovirus to a human subject, which identified adenovirus serotype has fewer pre-existing neutralizing antibodies in a human population than exist in the human population for an adenovirus of serotype 5, the method comprising: identifying the adenovirus serotype that has fewer pre-existing neutralizing antibodies in an in vitro cellular infection neutralization assay in which multiple wells of cells are infected with a test adenovirus serotype wherein each well comprises an individual serum sample from a plurality of individuals from a human population and the amount of neutralization or inhibition of the test adenovirus in the presence of serum is compared to a control comprising cells infected with the test adenovirus in the absence of serum and further compared to cells infected with the adenovirus of serotype 5 in the presence of each of the serum samples; selecting a test adenovirus serotype therefrom that is neutralized or inhibited in a lower percentage of the samples as compared to the percentage neutralization or inhibition observed for the human adenovirus serotype 5; and producing the recombinant, replication-defective adenovirus of the selected adenovirus serotype that comprises an adenoviral genome of the selected adenovirus serotype comprising a nucleic acid of interest operably linked to a promoter.