Patent ID: 7572577

Claim:
A method for RT-PCR recovery of cDNA from mRNA in ribosome display complexes, said method comprising: (a) reverse transcribing mRNA in ribosome display complexes or isolated from ribosome display complexes using an RT primer comprising a 5′ sequence which is identical to a 5′ consensus region of the mRNA or which has at least 80% homology to the 5′ consensus region of the mRNA and is capable of hybridizing specifically with DNA complementary to a part of the mRNA 5′region in the conditions under which the reverse transcription step is performed and comprising a 3′ primer region sequence complementary to a 3′ region of the mRNA, whereby single stranded cDNA is generated; and (b) amplifying by PCR the single stranded cDNA using a single type of primer which is identical to or overlapping with the 5′ sequence of the RT primer used, or which has at least 80% homology to the 5′consensus region of the mRNA and is capable of hybridizing specifically with DNA complementary to a part of the mRNA 5′ region in the conditions under which the reaction is performed.