Patent ID: 8126235

Claim:
A computer readable medium having processor-executable instructions stored thereon, the processor-executable instruction, when executed by at least one processor, performing a method of automated base-calling using at least one image obtained from a chemical sequencing process performed simultaneously on a plurality of DNA strands, the at least one image including intensity information corresponding to locations of at least one base in the plurality of DNA strands, the method comprising: processing the at least image to obtain a function corresponding to the intensity information in the at least one image for the at least one base, the function incorporating intensity information corresponding to each of the plurality of DNA strands; identifying a plurality of peaks in the function, the plurality of peaks indicating possible locations for the at least one base in the plurality of DNA strands; assigning membership to each of the plurality of peaks by determining whether each of the plurality of peaks is believed to have resulted from none, one or multiple of the plurality of DNA strands; and computing a sequence for the at least one base for each of the plurality of DNA strands based, at least in part, on the membership assignment.