Patent ID: 7178386

Claim:
A multi-column liquid chromatography data correction methods comprising the steps of: providing a plurality of liquid chromatography columns in fluid communication with a common mobile phase source, each column of the plurality of columns containing a stationary phase material, wherein the mobile phase source supplies a mobile phase to the plurality of columns at a selected mobile phase source output flow rate; providing at least one detector in sensory communication with the plurality of columns; supplying a calibrant having at least a first component to each column of the plurality of columns; eluting the at least a first component of the calibrant in each column of the plurality columns according to a first mobile phase source output flow rate and a first mobile phase composition profile; measuring, for each column of the plurality of columns, a first physical parameter using the at least one detector; deriving, for each column of the plurality of columns, at least one correction factor based on the first physical parameter; supplying, to each column of the plurality of columns, at least one sample containing a plurality of sample components; eluting at least two components of the plurality of sample components of the at least one sample in each column of the plurality of columns to obtain raw chromatographic data; and applying, for each column of the plurality of columns, the at least one correction factor to the raw chromatographic data to yield corrected chromatographic output data.