Patent ID: 8129113

Claim:
An analysis chip of at least one analyte present in a liquid sample, said chip comprising: (a) at least one analysis spot of said at least one analyte, said analysis spot being arranged on the chip in such a way as to permit the recognition and immobilization specific to the analyte; and (b) a reference range (G), said reference range comprising several reference spots each arranged on said chip in a defined manner and independently of one another, each reference spot of this range comprising, immobilized on its surface and in a defined proportion P different for each spot relative to the other reference spots of said range: (i) at least one probe reference molecule (PRM) for recognizing and specifically immobilizing a defined target reference molecule (TRM), and (ii) at least one inert molecule (IM) incapable of recognizing and attaching to said target reference molecule, the probe reference molecule and the inert molecule both being unable to recognize and immobilize said at least one analyte; with P = number ⁢ ⁢ of ⁢ ⁢ PR ⁢ ⁢ M number ⁢ ⁢ of ⁢ ⁢ P ⁢ ⁢ R ⁢ ⁢ M + number ⁢ ⁢ of ⁢ ⁢ I ⁢ ⁢ M ⁢ ⁢ and ⁢ ⁢ 0 ≤ P ≤ 1 and, the sum of the number of PRMs+number of IMs being constant from one reference spot to another, wherein the probe reference molecule (PRM), the target reference molecule (TRM), and the inert molecule (IM) are oligonucleotides, and do not recognize or immobilize the analyte.