Patent ID: 7695966

Claim:
A method of separating a multitude of biological objects, comprising: (a) each of the biological objects belong to one of at least two types of cells, (b) at least one of the types of cells has the property of having lower viscoelastic properties than the remaining types of cells, for clarity the objects having lower viscoelastic properties will be labeled the low viscoelastic objects, and the remaining objects will be labeled the high viscoelastic objects, (c) said biological objects if not already in a fluid are suspended in a fluid, (d) a first means for providing a chamber for holding the fluid, (e) a second means for providing a barrier, (f) said barrier has a plurality of holes of approximately equal to a predetermined diameter, (g) said diameter is chosen to be smaller than the majority of the low viscoelastic objects, (h) said chamber is separated by the barrier into an input side, and an output side of the chamber, (i) a third means to introduce said fluid into the input side of the chamber, (j) a fourth means for applying a controlled force to said fluid, (k) said controlled force kept lower than the predetermined force needed to force a majority of the low viscoelastic objects through the holes, (l) said controlled force is higher than the predetermined force needed to force a majority of the high viscoelastic objects through the holes, (m) said controlled force is applied to the fluid, thereby forcing the fluid, and the majority of the high viscoelastic objects to flow through the plurality of holes to the output side of the chamber, (n) whereby the majority of the low viscoelastic objects remain on the input side of the chamber, and the majority of the high viscoelastic objects have flowed through to the output side of the chamber, thereby separating the majority of the high viscoelastic objects from the majority of the low viscoelastic objects, wherein the biological objects are leukocytes and tumor cells.