Patent ID: 8301394

Claim:
A method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copies of a template nucleic acid molecule, comprising: (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction, wherein the signal is generated from the population of substantially identical copies of the template nucleic acid molecule; (b) generating a signal value for the signal; (c) repeating steps (a)-(b) for a plurality of sequence positions of the template nucleic acid molecule; (d) correcting a plurality of the signal values for a phasic synchrony error using a first parameter representative of an incomplete extension component of the phasic synchrony error that comprises a measure of one or more of the substantially identical copies of the template nucleic acid molecules behind an extension phase of the population and a second parameter representative of a carry forward component of the phasic synchrony error that comprises a measure of one or more of the substantially identical copies of the template nucleic acid molecules ahead of the extension phase of the population.