Patent ID: 7138254

Claim:
A method of performing an enzymatic reaction in a capillary tube using a normalized quantity of a nucleic acid, comprising: saturably binding a nucleic acid from an excess thereof directly on an inner surface of said capillary tube by contacting said inner surface with a solution comprising said nucleic acid and a chaotropic agent for a time sufficient for the nucleic acid to become saturably bound to said inner surface; removing unbound excess of the nucleic acid therefore generating a normalized quantity of said nucleic acid; introducing an enzymatic reaction mixture into said capillary tube having said normalized quantity of said nucleic acid; wherein said reaction mixture comprises an oligonucleotide primer, a DNA polymerase, dNPTs, and at least one dideoxynucleotide triphosphate (ddNTP), performing said enzymatic reaction in said capillary tube using said normalized quantity of said nucleic acid.