Patent ID: 8304191

Claim:
A sequencing system, the system comprising: (a) a reaction chamber, wherein the reaction chamber contains a sequencing reaction mixture comprising: (i) a template nucleic acid; (ii) a complementary nucleic acid that is at least partially complementary to the template nucleic acid, wherein the complementary nucleic acid is hybridized to the template nucleic acid; (iii) an exonuclease that comprises template dependent nucleobase binding activity, wherein the exonuclease is substantially free of nucleotide polymerization activity, and (iv) at least one labeled nucleobase; (b) a detector configured to detect a signal from the reaction chamber, which signal results from a transient template-dependent binding of a labeled nucleobase by the exonuclease in the reaction chamber; and, (c) a sequence assembly module that assembles template nucleic acid sequence information based upon detection of the signal.