Patent ID: 8911941

Claim:
A method for point-of-care amplification and detection of a target nucleic acid sequence, the method comprising: collecting a sample matrix with filter paper; advancing a punch element through the filter paper to form at least one punch of the filter paper; transferring the at least one punch of the filter paper to a reaction chamber comprising nucleic acid amplification reagents; sealing the at least one punch of the filter paper within the reaction chamber in fluid communication with the nucleic acid amplification reagents; heating the nucleic acid amplification reagents with a heating element in order to amplify the target nucleic acid sequence when contained within the sample matrix; detecting amplification of the target nucleic acid sequence with a sensor; displaying detection results obtained with the sensor on a display; and disposing of the at least one punch of the filter paper, the reaction chamber, the heating element, the sensor, the display and the nucleic acid amplification reagents after one-time use.