Patent ID: 7527928

Claim:
A system comprising a target nucleic acid comprising a first region and a second region, said second region downstream of and contiguous to said first region, oligonucleotides capable of hybridizing to said target nucleic acid to form an invasive cleavage structure, wherein said oligonucleotides comprise first and second oligonucleotides, where at least a portion of said first oligonucleotide is completely complementary to said first region of said target nucleic acid, and wherein said second oligonucleotide comprises a 3′ portion and a 5′ portion, wherein said 5′ portion is completely complementary to said second region of said target nucleic acid, a solid support, and a thermostable archaeal FEN-1 endonuclease, wherein said FEN-1 is active at 55° C. and above, for detecting the presence of an invasive cleavage structure; wherein one or more of said oligonucleotides is attached to said solid support, and wherein an invasive cleavage structure forms when a first and a second oligonucleotide hybridize to said first and second regions of said target nucleic acid.