Patent ID: 8012734

Claim:
An isolated Family 6 cellulase variant comprising at least one of the amino acid substitutions selected from the group consisting of: a basic, non-polar or proline residue at position 103 (X103H, K, R, A, V, L, P, or M) ; a valine or isoleucine residue at position 136 (X136V, or I); a tyrosine or lysine residues at position 186 (X186Y, or K); an acidic, glutamine or serine residue at position 365 (X365D, E, Q, or S); and an alanine, phenylalanine, leucine, glutamine or serine residue at position 410 (X410A, F, L, Q, or S), said position determined from alignment of a parental Family 6 cellulase with a Trichoderma reesei Cel6A amino acid sequence as defined in SEQ ID NO: 1 and wherein said isolated Family 6 cellulase variant comprises an amino acid sequence which is at least about 55% identical to amino acids 83-447 of SEQ ID NO: 1 and exhibits at least about 1.4-fold less inhibition by glucose than the parental Family 6 cellulase from which it is derived.