Patent ID: 7314625

Claim:
A chimeric protein comprising: a non-toxic Pseudomonas exotoxin A (PE) sequence and a Type IV pilin loop sequence, the Type IV pilin loop sequence being located within the PE sequences wherein the chimeric protein lacks ADP ribosylation activity and comprises: (a) the PE translocation domain or a portion of the PE translocation domain sufficient to effect translocation of the chimeric protein to a cell cytosol; and (b) a portion of the endoplasmic reticulum retention domain sufficient to translocate the chimeric protein from endosome to endoplasmic reticulum; and wherein the pilin loop sequence is located between the portion of the PE translocation domain and the portion of the PE endoplasmic reticulum retention domain; and wherein the Tppe IV pilin loop sequence is selected from the group consisting of SEQ ID NO:3, SEQ ID NO 4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10.