Patent ID: 7192725

Claim:
A method of determining the presence of a flagellin protein C (FlaC) of a flagellum of a Campylobacter strain in a sample, comprising the steps of: (a) immunizing a subject with an immunogenic composition to produce antibodies specific or said FlaC protein, the composition comprising (I) at least one active component selected from the group consisting of: (A) a recombinant FlaC protein of a strain of Campylobacter producible in a transformed host cell containing an expression vector comprising a purified and isolated nucleic acid molecule having a sequence selected from the group consisting of: (a) the entire nucleotide sequence set out in FIG. 1 (SEQ ID No: 1), or the complementary sequence of said sequence, (b) the coding nucleotide sequence set out in FIG. 1 (SEQ ID No: 2), or the complementary sequence of said sequence, and (c) a nucleotide sequence encoding the amino acid sequence set forth in FIG. 1 (SEQ ID No: 3) and expression means operatively coupled to the nucleic acid molecule for expression by the host cell of the recombinant FlaC protein; and (B) an isolated and purified FlaC protein of a Campylobacter strain free from other proteins of the Campylobacter strain, and (II) a pharmaceutically acceptable carrier therefor, said at least one active component producing an immune response when administered to a host, (b) isolating said antibodies from the subject, (c) contacting said sample with the antibodies to produce complexes comprising any FlaC protein of a Campylobacter strain present in the sample and said FlaC protein specific antibodies; and (d) detecting production of the complexes.