Patent ID: 7816104

Claim:
A method of preparing a cell comprising an adenovirus E1A coding sequence, an adenovirus E1B-19K coding sequence, and an adenovirus E1B-55K coding sequence integrated into the cell's genome, the method comprising the step of introducing into a precursor cell a nucleic acid molecule or molecules selected from the group consisting of: a) a single nucleic acid molecule comprising said adenovirus E1A coding sequence, said adenovirus E1B-19K coding sequence, and said adenovirus E1B-55K coding sequence, wherein at least two of said adenovirus E1A coding sequence, said adenovirus E1B-19K coding sequence, and said adenovirus E1B-55K coding sequence are separated by at least 8 kb; and b) two or more nucleic acid molecules together comprising the E1A and E1B-19K and E1B-55K coding sequences wherein said E1A and at least one of the E1B coding sequences are separated by at least 8 kb when said two or more nucleic acid molecules would form a single molecule by end-to-end joining before integrating into the precursor cell's genome.