Patent ID: 6962954

Claim:
A method for making a macromolecule M that is linked to a biomolecule B comprising the following steps: (a) providing a linking reagent represented by the formula where n=1 or 2; R is —NHC(O)—O-M; M is a reacted alcohol-containing macromolecule; and R is in a para-, meta- or di-meta position relative to —NCS; (b) providing an amine-containing biomolecule B in an admixture with the linking reagent provided in step (a) to form a linking mixture; and (c) maintaining said linking mixture for a time period sufficient to form a urethane compound represented by the chemical formula where n=1 or 2; R is —NHC(O)—O-M; M is a reacted alcohol-containing macromolecule; and R is in a para-, meta- or di-meta position relative to —N(H)C(S)N(H)—B thereby making a macromolecule M that is linked to a biomolecule B.