Patent ID: 7294755

Claim:
An in vivo method of incorporating exogenous genetic material into the genome of a mouse, said method comprising the steps of: (a) injecting into a vas efferens, or retes of a testis of a male mouse a gene delivery mixture comprising a retroviral vector comprises at least one optionally a gene encoding a genetic selection marker, operatively linked to a promoter sequence that a transcriptional unit is formed, under conditions effective to reach within the testis of a male germ cell selected from the group consisting of spermatogonial stem cells, type B spermatogonial, primary spermatocytes, preleptotene spermatocytes, leptotene spermatocytes, zygotene spermatocytes, pachytene spermatocytes, secondary spermatocytes, secondary spermatocytes, spermatids, and spermatozoa; (b) allowing the polynucleotide encoding a desired gene product to be incorporated into the genome of the male germ cell, so that a genetically modified male gamete is produced by the male mouse; and (c) breeding the male mouse with a female mouse, such that the genetically modified gametes is united with a female gamete and a transgenic progeny is produced thereby, that carries the polynucleotide encoding the desired gene product in its somatic cells.