Patent ID: 8093044

Claim:
A cell line expressing a botulinum neurotoxin substrate complex, wherein said complex comprises: (a) a peptide substrate selected from the group consisting of synaptosomal associated protein of 25 kD (SNAP-25), a SNAP-25 isoform, syntaxin, a syntaxin isoform, vesicle-associated membrane protein (VAMP), a VAMP isoform, and peptides having at least 80% identity to the foregoing, wherein said peptide substrate comprises a motif of soluble NSF (N-ethylmaleimide-sensitive fusion protein) attachment protein receptor (a SNARE motif); (b) a reporter domain comprising a fluorescent protein covalently attached to one side of said peptide substrate; and (c) an immobilization domain covalently attached to an opposite side of said peptide substrate.