Patent ID: 7670772

Claim:
A method of forming a microfluidic chip for a multiple bioassay, the method comprising: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate, wherein the sample channels and probe channels cross, forming probe immobilization regions at the intersections between the probe channels and the sample channels by photopolymerizing a polymer having biomolecule binding groups to the substrate, wherein the probe immobilization regions have biomolecule binding groups, and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target samples, wherein forming of blocking walls comprises disposing a polymer that does not have a biomolecule binding group before and after each of the probe immobilization regions by photopolymerizing the polymer that does not have the biomolecule binding group and the polymer that does not have the biomolecule binding group is a polyethyleneglycol diacrylate (PEG-DA) polymer.