Patent ID: 8124374

Claim:
A method of producing recombinant protein by using recombinant koji molds which are obtained by introducing a gene product obtained by ligating a gene encoding an intended protein to downstream of a native promoter of a gene encoding any enzyme selected from the group consisting of glucoamylase from Aspergillus kawachii, Aspergillus awamori, Aspergillus niger or Aspergillus oryzae , acid stable or-amylase from Aspergillus kawachii or Aspergillus niger , α-amylase from Aspergillus kawachii, Aspergillus awamori, Aspergillus niger or Aspergillus oryzae and acid protease from Aspergillus awamori, Aspergillus niger or Aspergillus oryzae , into white koji molds or black koji molds as host; or using recombinant koji molds which are obtained by introducing a gene product obtained by ligating a gene encoding an intended protein to downstream of a native promoter of a gene encoding any enzyme selected from the group consisting of glucoamylase from Aspergillus kawachii, Aspergillus awamori, Aspergillus niger or Aspergillus oryzae , α-amylase from Aspergillus kawachii, Aspergillus awamori, Aspergillus niger or Aspergillus oryzae , β-glucanase from Aspergillus kawachii, Aspergillus niger or Aspergillus oryzae and acid protease from Aspergillus awamori, Aspergillus niger or Aspergillus oryzae , into yellow koji molds as host: wherein the native promoter has a catabolite repression, comprising: culturing the recombinant koji molds, said recombinant koji molds not being in a solid koji, in a liquid medium which comprises barley and/or wheat having the surface of which entirely or partially covered with husks, wherein crushed products are excluded, thereby decreases the catabolite repression to expresses the intended protein; and collecting the recombinant protein from the culture product.