Patent ID: 7749721

Claim:
A method of immunoenzymatically detecting the presence of cholesterol esterase transferring protein (CETP) in a biological or synthetic sample, wherein said method does not detect a CETP variant without a cholesterol ester binding motif, said method comprising: (a) obtaining said sample; (b) contacting said sample with a primary polyclonal antibody under conditions sufficient for said primary antibody to bind to CETP, wherein said primary antibody is raised against the polypeptide of SEQ ID NO: 5; and (c) enzymatically detecting the presence of said primary antibody from step (b), by contacting said sample and said primary antibody with a secondary antibody, wherein said secondary antibody comprises an enzymatic detection agent; wherein detection of said primary antibody indicates the presence of CETP comprising the cholesterol ester binding motif in said sample while not detecting CETP variants without the cholesterol ester binding motif; and wherein said method further comprises a positive control, wherein said positive control is glutathione-S-transferase (GST) fused to the polypeptide of SEQ ID NO: 4.