Patent ID: 8137964

Claim:
A method of producing a three-dimensional tissue by cell lamination, the method comprising the steps of: (A) forming a cell layer; (B) bringing the cell layer into contact with a solution containing a first substance and a solution containing a second substance alternately, thus forming, on the cell layer, an extracellular matrix having a thickness of 1 to 100 nm in which the first substance and the second substance are laminated alternately; (C) culturing cells on the extracellular matrix to form a cell layer; and (D) repeating the steps (B) and (C) at least once, wherein in the step (A), the cell layer is formed on a substrate, in the step (D), the cell layer of the step (B) is formed on the extracellular matrix in the step (C), a combination of the first substance and the second substance is a combination of a protein having an RGD sequence and a protein that interacts with the protein having the RGD sequence, pH of the solution containing the first substance is 6 or more and 8 or less, pH of the solution containing the second substance is 6 or more and 8 or less, the step (B) is conducted after the step (A) or (C), and the step (C) is conducted after the step (B).