Patent ID: 8883753

Claim:
A method for expressing an apoAI polypeptide in a subject comprising administering to said subject a pre-trans-splicing molecule comprising: (a) one or more target binding domains that target binding of the pre-trans-splicing molecule to human albumin pre-mRNAs expressed within a cell; (b) a 3′ splice region comprising a branch point and a 3′ splice acceptor site; (c) a spacer region that separates the 3′ splice region from the target binding domain; (d) a safety nucleotide sequence comprising one or more complementary sequences that bind to one or more sides of the 5′ splice region; and (e) a nucleotide sequence to be trans-spliced to the target pre-mRNA wherein said nucleotide sequence encodes an apoAI; wherein said pre-trans-splicing molecule is recognized by nuclear splicing components within the cell.