Patent ID: 8597889

Claim:
A method for detecting a cancer cell caused by HPV, comprising the steps of: (A) preparing a sample containing DNA from a cell of a subject; (B) converting unmethylated cytosine in DNA contained in the sample obtained in the step (A) into another base, to give a conversion sample such that in a nucleic acid in the L1 region or L2 region of HPV, cytosine present in a CpG site is not converted into another base, and in a nucleic acid sequence in the LCR or E6 region of HPV, a cytosine in a CpG site is converted into another base; (C) carrying out nucleic acid amplification reaction, by using the conversion sample obtained in the step (B), a first primer which hybridizes with said nucleic acid in the L1 region or L2 region of HPV, and a second primer which hybridizes with said nucleic acid in the LCR or E6 region of HPV, wherein the nucleic acid amplification reaction is to amplify a nucleic acid consisting of a continuous nucleotide sequence ranged from the site with which the first primer is hybridized to the site with which the second primer is hybridized; and (D) detecting a cancer cell caused by HPV based on the result of the nucleic acid amplification reaction of the step (C).