Patent ID: 8432544

Claim:
A Method for detecting BPA by Surface Enhanced Raman Spectroscopy, comprising: (1) soaking and ultrasonically extracting bisphenol A present in a sample with an organic solvent to form an extraction solution, adding methanol to the extraction solution to cause a polymer to precipitate from the extraction solution and leaving a filtrate behind, filtering and concentrating the filtrate, diluting the concentrated filtrate to volume with methanol, and filtering the diluted filtrate through a filter with a pore size of 0.45 μm to obtain a pretreated sample; (2) reducing KAuCl 4 with sodium citrate to obtain colloidal gold with evenly-distributed particle sizes; (3) detecting bisphenol A present in the sample by Raman spectroscopy, wherein spectral conditions for Raman spectroscopy include an incident laser power of 100 to 300 mW, and a scan time of 2 to 20 seconds; and (4) mixing the pretreated sample obtained in step (1) with the colloidal gold obtained in step (2) in an appropriate ratio, followed by adjusting pH value with an acid or a base, and then carrying out the detection of bisphenol A present in the sample by Raman spectroscopy under the spectral conditions shown in step (3).