Patent ID: 8569015

Claim:
A method of producing a soluble recombinant Bacillus anthracis protective antigen protein (rPA) in a Pseudomonas fluorescens expression system comprising expressing the soluble rPA from a nucleotide sequence encoding the soluble rPA, wherein the nucleotide sequence is the soluble rPA encoding sequence of a nucleotide sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, and SEQ ID NO:14, or wherein the nucleotide sequence encoding the soluble rPA is a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO:2, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, or SEQ ID NO:14.