Patent ID: 8394620

Claim:
A two-component genome flavivirus composition, comprising: a first virus particle comprising a first viral genome encoding (i) an amino-terminal capsid fragment having more than the first 20 and at most the first 25 amino acids of the capsid protein, wherein the capsid fragment is not capable of nucleocapsid formation, (ii) a 5′ UTR, (iii) cis-acting promoter elements required for RNA replication, (iv) envelope proteins, and (v) a complete set of non-structural proteins of the flavivirus; and a second virus particle comprising a complementing second viral genome encoding (i) a 5′ UTR, (ii) cis-acting promoter elements required for RNA replication, (iii) capsid protein, and (iv) a complete set of non-structural proteins of the flavivirus.