Patent ID: 7758871

Claim:
A method of reducing neurotransmitter release in a subdermal structure of a patient, the method comprising the steps of: (a) non-chemically disrupting the stratum corneum of the patient's skin to reduce impermeability of the stratum corneum: (b) applying a fluid to the patient's skin; (c) applying a transdermal patch to the skin of the patient in an area that had the stratum corneum disrupted in step (a), the transdermal patch comprising; (i) a pharmaceutical composition comprising a stabilized botulinum toxin provided in a dried state and an enhancing agent that is mixable with the stabilized botulinum toxin provided in a dried state and facilitates transdermal administration of a botulinum toxin in a bioactive form to a subdermal target site of a human patient without being administered to the patient's circulatory systems; and (ii) an adhesive layer disposed to one side of the transdermal patch to removably secure the patch on the patient's skin; wherein the pharmaceutical composition is incorporated into the adhesive layer; and (d) solubilizing the botulinum toxin provided in the dry state with the fluid, wherein solubilization of the botulinum toxin permits diffusion of the botulinum toxin from the adhesive layer into the patient's skin thereby reducing neurotransmitter release in a subdermal structure.