Patent ID: 7675626

Claim:
A method of detecting a microorganism sensitive to a drug by a surface plasmon resonance device comprising (a) a prism coated with a metal film on the bottom of the prism or a metal film with grating, (b) a chamber for flowing through the microorganism wherein the chamber is contacted with the film, (c) an immobilized material coated on the inner layer of the chamber, (d) a light source which is projected on the film to generate an output light, (e) a light detector to receive the output light, and (f) a device to analysis surface plasmon resonance angle shift, the method comprises (1) providing a sample and a control group wherein the control group is drug susceptible or wild type, (2) flowing the control group and the sample through the chamber respectively to be captured by the immobilized material, (3) adding the drug into the chamber, (4) recording the data selected from the group consisting of (i) the value V 1 of surface plasmon resonance angle shift of the control group and the value V 2 of surface plasmon resonance angle shift of the sample, (ii) the value D 1 of the drop of surface plasmon resonance angle shift of the control group and the value D 2 of the drop of surface plasmon resonance angle shift of the sample, and (iii) the surface plasmon resonance angle shift of the control group and the sample, and (5) identifying the sample as drug resistance by the record selected from the group consisting of (I) ratio of V 1 /V 2 larger than 1.001, (II) ratio of D 1 /D 2 larger than 1.001, and (III) the surface plasmon resonance angle shift of the sample more smooth or monotonic than that of the control.