Patent ID: 7264702

Claim:
A method for separating analytes and determining a total analyte concentration or amount, the method comprising: (i) electrokinetically flowing at least two analytes through a first channel; (ii) waiting a specified time, during which specified time the at least two analytes flow into an intersection of the first channel and a separation channel; (iii) injecting the at least two analytes at the intersection into the separation channel; (iv) separating the at least two analytes in the separation channel, which separating results in two or more separated analytes; (v) detecting the two or more separated analytes, which detecting results in two or more individual analyte signals; and, (vi) determining a total analyte concentration or amount from the two or more individual analyte signals, wherein each of the two or more signals comprises an area or peak height, and wherein the determining step comprises summing the areas of the two or more signals or summing the peak heights of the two or more signals to determine the total analyte concentration or amount.