Patent ID: 7736851

Claim:
A method for cloning DNA molecules in procaryotic cells comprising the steps of: a) providing a procaryotic host cell capable of performing RecET homologous recombination, wherein the host cell expresses redα and redβ genes, b) contacting in said host cell a circular first DNA molecule which is capable of being replicated in said host cell with a linear second DNA molecule comprising at least two regions of sequence homology to regions on the first DNA molecule and further comprising a DNA fragment to be cloned under conditions which favour homologous recombination between said first and second DNA molecules and c) selecting a host cell in which homologous recombination between said first and second DNA molecules has occurred, wherein a second DNA molecule is introduced into the host cell in a form which allows recombination without further modification; and wherein when said RecET homologous recombination occurs, it is mediated by gene products of said redα and redβ genes.