Patent ID: 8778844

Claim:
A method for preparing one or more peptide ligands, comprising polypeptides covalently linked to tris-(bromomethyl) benzene (TBMB) at two or more amino acid residues, comprising the steps of: (a) displaying one or more peptide ligand in a phage display system, wherein the displayed polypeptide comprises two or more reactive groups, wherein the two or more reactive groups are conjugated to TBMB to form a covalent linkage to TBMB, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; wherein the TBMB is present at less than 100 μM during conjugating to the phage displayed polypeptide; (b) exposing the phage displayed peptide ligands covalently linked to TBMB to one or more proteases; and (c) screening the phage displayed peptide ligands covalently linked to TBMB for binding against a target, and selecting the phage displayed peptide ligands covalently linked to TBMB which bind to the target.