Patent ID: 8507244

Claim:
An isolated nucleic acid encoding a variant of a parent Bacillus sp. TS-23 alpha-amylase, selected from the group consisting of: (A) a variant of a parent Bacillus sp. TS-23 alpha-amylase, wherein the variant comprises an amino acid sequence with at least 90% amino acid sequence identity to SEQ ID NO: 2, wherein the variant has amylase activity, and wherein said variant comprises a truncation of the C-terminus, the modification S243Q, A, E, or D, and optionally any one or more of modifications (a) to (i), wherein each amino acid position corresponds to a position in SEQ ID NO: 1: (a) R180 deletion or S181 deletion or R180 and S181 deletion; (b) M201L; (c) Q87 to E or R; (d) N225 to E or R; (e) N272 to E or R; (f) N282 to E or R; (g) T182 deletion; (h) G183 deletion; or (i) Q98R, M201L, S243Q, R309A, Q320R, Q359E, and K444E; and (B) a variant of a parent alpha-amylase, wherein said variant is encoded by a nucleic acid that hybridizes under high stringency conditions to a nucleic acid complementary to a nucleic acid encoding SEQ ID NO: 2, wherein said hybridization conditions involve pre-soaking in 5×SSC and prehybridizing for 1 hour at 40° C. in a solution of 20% formamide, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 mg of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 100 mM ATP for 18 hours at 40° C., followed by three times washing in 2×SSC, 0.2% SDS at 65° C., where the variant has alpha amylase activity, the modification S243Q, A, E, or D, and optionally any one or more of modifications (a) to (i), wherein each amino acid position corresponds to a position in SEQ ID NO: 1: (a) R180 deletion or S181 deletion or R180 and S181 deletion; (b) M201L; (c) Q87 to E or R; (d) N225 to E or R; (e) N272 to E or R; (f) N282 to E or R; (g) T182 deletion; (h) G183 deletion; or (i) Q98R, M201L, S243Q, R309A, Q320R, Q359E, and K444E.