Patent ID: 7745607

Claim:
A method for selecting aptamer capable of binding to a target, said method comprising the steps of: i) interacting antisense oligonucleotide having the nucleotide sequence of SEQ ID NO: 1 and being adapted to be attached to a solid support with an oligonucleotide library containing multiple oligonucleotides, each of which includes an antisense binding domain having a sequence complementary to SEQ ID NO: 1 and a random nucleotide domain, wherein the antisense oligonucleotide and the oligonucleotides in the library form double stranded duplexes, ii) immobilizing the duplexes on a solid support; iii) incubating the duplexes in the presence of the target; and iv) collecting library oligonucleotides that dissociate from the duplexes and bind to the target.