Patent ID: 7811451

Claim:
A monolith type separation column including a monolith rod which is cylindrically formed with a porous material and into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of the monolith rod; a support member into which the monolith rod coated with the coating material is inserted; a rod fixing material filling a gap between the coating material and the support member; a sealing member for preventing said sample from flowing into said rod fixing material, said sealing member having a tapered upper outer surface, a tapered lower outer surface, and an inner surface, and said sealing member contacting an outer peripheral surface of said coating material, an inflow-side end surface of said rod fixing material, an outflow-side end surface of said rod fixing material, and said support member to seal said inflow-side end surface and said outflow-side end surface of said rod fixing material; and a push-on member for pushing and pressing said sealing member toward said outer peripheral surface of said coating material, said inflow-side end surface of said rod fixing material, said outflow-side end surface of said rod fixing material, and said support member, said push-on member having a tapered inner surface that engages said tapered upper outer surface of said sealing member; a diffusion member disposed on an upstream side of the monolith rod for introducing the sample to the monolith rod while allowing diffusion of the sample; and a packing member for pushing and pressing the push-on member toward said sealing member and having an inner circumference surrounding the diffusion member, wherein said rod fixing material is formed by inserting a fluid substance between said coating material and said support member and by hardening said fluid substance, and wherein said tapered inner surface of said push on member engages said tapered upper outer surface of said sealing member and presses said sealing member into contact with said outer peripheral surface of said coating material.