Patent ID: 7829684

Claim:
A method for expressing or producing a recombinant multiple-membrane-spanning 4-domain subfamily (MS4A) polypeptide in a bacterial host cell, comprising: (a) providing a nucleic acid comprising: (i) a polynucleotide encoding the MS4A polypeptide or a membrane-spanning fragment of the MS4A polypeptide; (ii) a phac promoter consisting of SEQ ID NO:15 or tphac promoter consisting of SEQ ID NO:16 operatively linked to the polynucleotide encoding the MS4A polypeptide of (i); and (iii) a polynucleotide encoding a translation initiation enhancer; (b) introducing the nucleic acid into the host cell; and (c) expressing the nucleic acid in the host cell to express and produce the MS4A polypeptide or the membrane-spanning fragment of the MS4A polypeptide.