Patent ID: 7090003

Claim:
A microfludic device for regulating the temperature of a sample fluid, comprising: a body member defining a fluid channel for receiving the sample fluid therein, a maintenance channel, a first regulating channel, and a second regulating channel, the fluid channel extending generally along a longitudinal axis and the first regulating channel including a first portion being a first predetermined distance from the fluid channel and a second portion being a second predetermined distance from the fluid channel; a first regulating fluid having a predetermined temperature and being flowable within the first regulating channel, the first regulating fluid effectuating a heat exchange with the sample fluid in the fluid channel; a second regulating fluid having a predetermined temperature and being disposed in the second regulating channel, the second regulating fluid effectuating a heat exchange with the sample fluid in the fluid channel; and a maintenance fluid in the maintenance channel, the maintenance fluid effectuating a heat exchange with the regulating fluid in the first regulating channel so as to maintain the temperature thereof.