Patent ID: 7807410

Claim:
A method of cloning and expressing the cold-inducible hutU gene from an Antarctic Psychotropic Bacterium Pseudomonas syringae , said method comprising the steps of: a. cloning a 2.4 kbp Pst DNA fragment containing the hutU gene, and three overlapping fragments containing an upstream 3.5 kbp Eco-R1-Kpn 1 fragment, a downstream 2.54 Kbp SalI and a 1.2 Kbp pstI fragments into pUC19, b. sequencing the clone, and c. expressing the sequenced clone in the Antarctic Psychotropic Bacterium Pseudomonas syringae; wherein the fragments comprise a DNA sequence (SEQ. ID No.: 18) from nucleotide 2961 to 3600 of the upstream region of the cold-inducible hutU gene, wherein said DNA sequence comprises a promoter and other regulatory sequences with a CAAAA nucleotide sequence at −10 site of multiple transcription initiation sites.