Patent ID: 7468269

Claim:
A recombinant organism comprising a recombinant vector comprising: (a) a λ exo and a λ bet nucleotide sequences encoding bacteriophage λ Red recombinase; (b) a λ gam nucleotide sequence encoding bacteriophage anti-RecBCD; (c) a Ptac promoter sequence operably linked to the nucleotide sequence of (a) and (b); and (d) a nucleotide sequence encoding LacI operably linked to its native promoter; and (e) at least one origin of replication sequence which confers low copy number on the vector, wherein the recombinant organism is a bacterial species which is pathogenic to humans, animals, or plants, and whereby the products of the recombinant vector facilitate replacement of genomic nucleic acid in the recombinant organism with substrate nucleic acid.