Patent ID: 7419660

Claim:
A cell expressing a multivalent composition, said multivalent composition for active idiotype immunotherapy, said cell produced according to a method comprising: a) providing: i) malignant B cells isolated from a patient having a quasi-clonal B-cell lymphoma; ii) at least one expression vector; iii) an amplification vector comprising a recombinant oligonucleotide having a sequence encoding a first inhibitable enzyme operably linked to a heterologous promoter; and iv) a T lymphoid parent cell line; b) isolating nucleic acid from said malignant cells, said nucleic acid comprising nucleotide sequences selected from the group consisting of nucleotide sequences encoding at least one V H region and at least two V L regions, nucleotide sequences encoding at least two V H regions and at least one V L region, and nucleotide sequences encoding at least two V H regions and at least two V L regions, wherein said at least two V L regions differ by at least one idiotope, wherein said at least two V H regions differ by at least one idiotope, and wherein said V H and V L regions are derived from immunoglobulin molecules expressed by said malignant cells; c) inserting said nucleic acid comprising nucleotide sequences encoding said V H and V L regions into said at least one expression vector; d) introducing said at least one expression vector and said amplification vector into said parent cell line to generate one or more transformed cells; e) growing said transformed cells in a first aqueous solution containing an inhibitor capable of inhibiting said first inhibitable enzyme wherein the concentration of said inhibitor present in said first aqueous solution is sufficient to prevent growth of said parent cell line; and f) identifying a transformed cell capable of growth in said first aqueous solution, wherein said transformed cell capable of growth expresses a combination of V H and V L regions selected from the group consisting of at least one V H region and at least two V L regions, at least two V H regions and at least one V L region, and at least two V H regions and at least two V L regions, wherein said at least two V L regions differ by at least one idiotope, wherein said at least two V H regions differ by at least one idiotope, and wherein said V H and V L regions comprise a protein molecule, wherein said multivalent composition comprises said expressed V H and V L regions.