Patent ID: 8334109

Claim:
A reagent for blood analysis, said reagent comprising: (1) a compound having the general formula I: wherein R 1 and R 2 are each independently selected from at least one of the following: C 1-18 malkylCOOR 6 , C 1-18 alkylOR 6 and benzyl, wherein said benzyl is optionally substituted with a substituent selected from at least one of the following: halogen, hydroxyl, sulfhydryl, cyano, nitro, alkyl, aryl, alkyloxy, heterocyclyl, haloalkyl, amino, alkylamino, amido and carboxyl, provided that R 1 and R 2 are not all simultaneously benzyl; R 6 in each occurrence is independently C 1-18 alkyl or phenyl, wherein said phenyl is optionally substituted with a substituent selected from at least one of the following: halogen, hydroxyl, sulfhydryl, cyano, nitro, alkyl, aryl, alkyloxy, heterocyclyl, haloalkyl, amino, alkylamino, amido and carboxyl; X is CH 2 , C(CH 3 ) 2 , O, S or Se; Y is —CH—CH═C—, are each independently R 3 , R 4 and R 5 are each independently selected from at least one of the following: H, halogen, cyano, hydroxyl, C 1-18 alkyl, C 1-18 alkylsulphonyl, sulphonyl and C 1-5 alkylCOOR 7 ; R 7 is H or C 1-6 alkyl; and Z − is an anion; wherein the compound of formula I is present in a concentration of from about 0.01 ppm to about 1000 ppm and configured to permit detection of erythroblasts and basophils; and (2) at least one surfactant selected from cationic surfactants and nonionic surfactants, wherein the cationic surfactant is selected from quaternary ammonium salt-type cationic surfactants having the following general formula II: wherein R 8 is alkyl or alkenyl having 6 to 14 carbon atoms; R 9 and R 10 are alkyl or alkenyl having 1 to 4 carbon atoms; R 11 is alkyl or alkenyl having 1 to 4 carbon atoms, or benzyl; and B is a halogen atom; and the nonionic suractant is selected from at least one of the following: octylphenylpolyoxyethylene ether, polyoxyethylene(10)cetyl ether, polyoxyethylene(23)cetyl ether, polyoxyethylene(25)cetyl ether and polyoxyethylene(30)cetyl ether; wherein the at least one surfactant is present in a concentration of from about 50 mg/L to about 5000 mg/L and is configured to lyse erythrocytes.