Patent ID: 7195870

Claim:
A method for diagnosis and/or prognosis of, or predisposition to a condition or disease comprising: converting, in a genomic DNA sample and by chemical treatment, cytosine bases that are unmethylated at the 5-position to uracil or another base dissimilar to cytosine in terms of hybridization behavior to provide chemically pretreated genomic DNA; amplifying fragments of the chemically pretreated genomic DNA having a sequence selected from the group consisting of SEQ ID NOS:49, 50 and complements, and portions thereof, using at least one set of primer oligonucleotides and a polymerase to produce amplificates; hybridising the amplificates to at least one set of detection oligomers; detecting the hybridised amplificates; and determining, based on the detection, the methylation status of at least one CpG dinucleotide of the CBFB genomic sequence corresponding to treated SEQ ID NOS:49, 50 and complements thereof, wherein a diagnosis and/or prognosis of, or predisposition to a condition or disease associated with CBFB is afforded, and wherein the condition or disease is a CBFB-associated cancer.