Patent ID: 7414117

Claim:
A nucleotide derivative having a fluorescent dye intercalator bound via a linker to a pyrimidine base or purine base, which, when existing as a member of a single-stranded sequence selected from the group consisting of: (1) a thymine/uracil derivative represented by Formula (1) wherein the fluorescent dye emits light most intensely when the confronting base is adenine; (2) a cytosine derivative represented by Formula (2) wherein the fluorescent dye emits light most intensely when the confronting base is guanine; (3) an adenine derivative represented by Formula (3) wherein the fluorescent dye emits light most intensely when the confronting base is cytosine; (4) a guanine derivative represented by Formula (4) wherein the fluorescent dye emits light most intensely when the confronting base is cytosine or thymine/uracil; and (5) an adenine derivative represented by Formula (5) wherein fluorescent dye emits light most intensely when the confronting base is thymine/uracil, wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 and R 9 for Formulae (1)-(5) are the same or different and each denotes a hydrogen atom or a substituent, R 10 denotes a hydrogen atom or a hydroxyl group, X denotes a linker group selected from the group consisting of an imino group (NH), an oxy group (O), a thio group (S), a methylene group (CH 2 ) and an alkylamino group, and n represents the length of the alkylene chain and is 0 to 5 when X is a methylene or alkylamino group and 1 to 5 when X is an imino, oxy or thio group.