Patent ID: 7790432

Claim:
A polypeptide having alanine 2,3-aminomutase (AAM) activity and having an amino acid sequence selected from the group consisting of: (a) an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, and 51; (b) an amino acid sequence which has at least 95% homology with the amino acid sequence of SEQ ID NO: 34, wherein the amino acid sequence of the AAM polypeptide comprises an amino acid residue selected from the group consisting of L at position 177, M at position 227, R at position 308, L at position 408, S at position 416, and G at position 447; and (c) an amino acid sequence encoded by a nucleic sequence which hybridizes under high stringency conditions with the complement of the nucleotide sequence of SEQ ID NO: 33, wherein the encoded amino acid sequence comprises an amino acid residue selected from the group consisting of L at position 177, M at position 227, R at position 308, L at position 408, S at position 416, and G at position 447, wherein the amino acid positions refer to the corresponding positions in SEQ ID NO:34, and wherein the high stringency conditions are hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA and 50% formamide, and washing three times each for 15 minutes using 2× SSC, 0.2% SDS at a temperature of at least 65° C.