Patent ID: 8859298

Claim:
An in vitro method for detection of a pathogenic conformational isomer of the prion protein (PrP SC ) in a sample, said method comprising the steps consisting of: a) putting nanobeads covered with plasminogen into contact with a sample; b) separating said nanobeads covered with plasminogen bearing the possibly present PrP SC , from the sample; c1) putting said nanobeads covered with plasminogen bearing the possibly present PrP SC obtained in step b) in contact with a preparation comprising some non-pathogenic conformational isomer of the prion protein (PrP c ); c2) disaggregating the aggregates possibly formed during step c1); d) detecting the presence of PrP SC in the preparation, the presence of PrP SC in the preparation being indicative of the presence of PrP SC in the sample; the steps c1) and c2) forming a cycle which is repeated at least twice before applying step d).