Patent ID: 8741356

Claim:
A process of preparing a canola protein isolate, which comprises: (a) extracting a canola oil seed meal at a temperature of at least about 5° C. to cause solubilization of protein in said oil seed meal and to form an aqueous canola protein solution having a protein content of about 5 to about 25 g/L and a pH of about 5 to about 6.8, (b) separating the aqueous canola protein solution from residual canola oil seed meal, (c) increasing the protein concentration of said aqueous canola protein solution to at least 250 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a concentrated canola protein solution, (d) diluting said concentrated canola protein solution into chilled water having a temperature below about 15° C. to cause the formation of canola protein micelles, (e) settling the canola protein micelles to form an amorphous, sticky, gelatinous, gluten-like canola protein micellar mass, and (f) separating the canola protein micellar mass from supernatant said canola protein micellar mass having a protein content of at least about 100 wt % as determined by Kjeldahl nitrogen×6.25 on a dry weight basis.