Patent ID: 8465959

Claim:
A method for producing a secreted, mature polypeptide, the method comprising: (a) cultivating a recombinant host cell comprising a polynucleotide encoding a protein comprising: (i) a heterologous pro-region comprising a sequence having at least 90% sequence identity to SEQ ID NO:30 and having activity to enhance expression of the protein, (ii) a signal peptide comprising a sequence encoded by a polynucleotide having at least 90% sequence identity to residues 1-81 of SEQ ID NO:2, and (iii) an amino acid sequence having at least 92% sequence identity to residues 166-353 of SEQ ID NO:43 and that has protease activity, and (b) culturing the host cell so as to produce a culture supernatant comprising the secreted, mature polypeptide, said secreted mature polypeptide lacking the heterologous pro-region and the signal peptide, and comprising the sequence having at least 90% sequence identity to residues 166-353 of SEQ ID NO:43 , wherein said sequence has protease activity, and (c) recovering the secreted, mature polypeptide.