Patent ID: 8097409

Claim:
A kit for amplifying and detecting a Streptococcus agalactiae target nucleic acid sequence, said kit comprising: a first primer, the base sequence of which consists of a 3′ terminal target-complementary sequence and a first primer upstream sequence comprising a promoter sequence for an RNA polymerase, said 3′ terminal target-complementary sequence of said first primer being contained within and comprising at least 25 contiguous bases of SEQ ID NO:2 or the full complement thereof, allowing for the presence of RNA base equivalents and nucleotide analogs; a second primer, the base sequence of which consists of a 3′ terminal target-complementary sequence contained within and comprising at least 20 contiguous bases of SEQ ID NO:1 or the full complement thereof, allowing for the presence of RNA base equivalents and nucleotide analogs; and a probe, the base sequence of which consists of a target-complementary sequence and, optionally, one or more base sequences that are non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said target-complementary sequence of said probe being contained within and comprising at least 16 contiguous bases of SEQ ID NO:3 or the full complement thereof, allowing for the presence of RNA base equivalents and nucleotide analogs.