Patent ID: 8288158

Claim:
A mammalian stem cell not in a human and comprising a recombinant, functional L-threonine 3-dehydrogenase (EC 1.1.1.103; TDH) gene, wherein: the TDH gene is a transgene; the TDH gene comprises an endogenous-to-the-cell TDH allele operably-linked to a heterologous-to-the-cell transactivator, wherein expression of the gene is activated by an exogenous-to-the-cell activator; the TDH gene is heterologous to the cell; the TDH gene is human, wherein human gene mutations at the AG to GG splice acceptor mutations in exons 4 and 6, as well as the non-sense mutation within exon 6 wherein arginine codon 214 is replaced by a translational stop codon, are corrected to impart L-threonine 3-dehydrogenase catalytic activity to the L-threonine 3-dehydrogenase encoded by the TDH gene; or the TDH gene is over-expressed, as compared with a corresponding wild-type TDH gene, and comprises a coding or transcription regulatory sequence heterologous to the cell.