Patent ID: 8257938

Claim:
A method of detecting the presence of an antibody against Anaplasma phagocytophilum in a biological sample of a mammal, comprising: (i) immobilizing an isolated polypeptide onto a surface, wherein said isolated polypeptide has the amino acid sequence set forth in SEQ ID NO: 17, SEQ ID NO:19, SEQ ID NO. 21, or SEQ ID NO. 23, (ii) contacting said isolated polypeptide with a biological sample of a mammal, under conditions that allow formation of an antibody-antigen complex, said biological sample containing an antibody against Anaplasma phagocytophilum ; and (iii) detecting the formation of said antibody-antigen complex, wherein said detected antibody-antigen complex is indicative of the presence of said antibody against Anaplasma phagocytophilum in said biological sample.