Patent ID: 8257705

Claim:
An isolated single domain antibody binding specifically to a preselected antigen comprising a variable heavy domain (VHH or VH) of an antibody, wherein amino acid residues of the VL interface of the variable heavy domain (VHH or VH) are i) Gly at position 44 according to Kabat numbering, Leu at position 45 according to Kabat numbering, and Trp at position 47 according to Kabat numbering, or ii) Gly at position 44 according to Kabat numbering, Leu at position 45 according to Kabat numbering, and Tyr at position 47 according to Kabat numbering, or iii) Gly at position 44 according to Kabat numbering, Phe at position 45 according to Kabat numbering, and Trp at position 47 according to Kabat numbering, or iv) Gly at position 44 according to Kabat numbering, Pro at position 45 according to Kabat numbering, and Trp at position 47 according to Kabat numbering, wherein in the variable heavy domain (VHH or VH), the amino acid at position 6 is Ala, the amino acid at position 74 is Ala, the amino acid at position 83 is Lys, the amino acid at position 84 is Pro, and the amino acid at position 108 is Gln, said positions according to Kabat numbering; and wherein the CDR3 region of the variable heavy domain (VHH or VH) is selected from the group consisting of: AHGGYGAFGS (SEQ ID NO: 119); YSGGALDA (SEQ ID NO: 122); LSQGAMDY (SEQ ID NO: 124); IDRERAFTS (SEQ ID NO: 127); IDWERAFTS (SEQ ID NO: 128); QGYAGSYDY (SEQ ID NO: 129); LGVPGTFDY (SEQ ID NO: 130); TNRGIFDY (SEQ ID NO: 131); TPGSSGVYEY (SEQ ID NO: 132); TQTGSHDY (SEQ ID NO: 133); QVGTAYDY (SEQ ID NO: 134); and RRGSSGVYEY (SEQ ID NO: 135).