Patent ID: 8889391

Claim:
A recombinant microorganism comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes a protein variant that comprises at least 95% sequence identity to SEQ ID NO: 4 and further comprises a His substitution at the amino acid corresponding to position 3 of SEQ ID NO: 4 or a conservative substitution of His, a Thr substitution at the amino acid corresponding to position 24 of SEQ ID NO: 4 or a conservative substitution of Thr, an Asn substitution at the amino acid corresponding to position 110 of SEQ ID NO: 4 or a conservative substitution of Asn, a Leu substitution at the amino acid corresponding to position 113 of SEQ ID NO: 4 or a conservative substitution of Leu, a Thr substitution at the amino acid corresponding to position 133 of SEQ ID NO: 4 or a conservative substitution of Thr, or combinations thereof, and wherein the protein has beta-alanine aminotransferase (BAAT) activity, and wherein the microorganism produces 3-hydroxypropionic acid (3-HP).