Patent ID: 8343721

Claim:
A method for synthesizing at least one as RNA molecule directly from at least one sRNA molecule, comprising: a) providing at least one sRNA molecule having a 5′ end and a 3′ end, said 3′ end comprising a first nucleotide sequence corresponding to the partial 5′ end of the template strand of a RNA polymerase recognition sequence; b) annealing to the 3′ end of said sRNA molecule a primer having a 5′ end and a 3′ end, said primer comprising a second nucleotide sequence corresponding to at the 5′ end of the non-template strand of said RNA polymerase recognition sequence sufficient in length to anneal to said first nucleotide sequence corresponding to the partial 5′ end of the template strand of said RNA polymerase recognition sequence; c) extending the 3′ end of said primer such that a double stranded RNA/DNA duplex is formed; d) degrading at least the 3′ portion of the RNA strand of said double stranded RNA/DNA duplex, thereby providing at least a partially single stranded DNA molecule having a single stranded 5′ end, said 5′ end comprising a third nucleotide sequence corresponding to the complete non-template strand of said RNA polymerase recognition sequence; e) synthesizing at least a partially double stranded DNA molecule from said at least partially single stranded DNA molecule such that said third nucleotide sequence corresponding to the complete non-template strand of said RNA polymerase recognition sequence is converted into a double stranded RNA polymerase promoter; and f) initiating RNA transcription using an RNA polymerase which recognizes said double stranded RNA polymerase promoter, thereby synthesizing at least one as RNA molecule directly from at least one sRNA molecule.