Patent ID: 8124144

Claim:
A method of making a purified anti-H lecithin, comprising the steps of: grinding seeds of bitter gourd to obtain a first powder; adding the first powder to distilled water followed by stirring for three hours to obtain a first mixture; filtering the first mixture to obtain a filtrate; centrifuging the filtrate to obtain a first supernatant; adding the first supernatant to ethanol followed by stirring, then left to stand for one hour to obtain a second mixture; removing a second su ernatant of the second mixture to obtain a first deposit; centrifuging the first deposit to obtain a second deposit; drying the second deposit to obtain a second powder; adding the second powder to a normal saline solution containing gelatin to obtain a third mixture; centrifuging the third mixture to obtain the purified anti-H lecithin, wherein the purified anti-H lecithin is used as a blood type checking reagent.