Patent ID: 8262887

Claim:
A method for increasing measurement precision of two-dimensional protein electrophoresis, essentially comprising steps of: (a) measuring a weight M exp of a protein sample; (b) measuring an electrical conductivity S exp of salts in the protein sample; (c) providing a set of equations for calculating a total electrical energy to be supplied to the protein sample, the set of equations including: Vh protein_exp = Vh protein_ref × M exp M ref × strip ⁢ ⁢ length exp strip ⁢ ⁢ length ref × pH ⁢ ⁢ range ref pH ⁢ ⁢ range exp ; ( c1 ) Vh salt_exp = Vh salt_ref × S exp - S blank S ref - S blank × strip ⁢ ⁢ length exp strip ⁢ ⁢ length ref × pH ⁢ ⁢ range ref pH ⁢ ⁢ range exp ; ( c ⁢ ⁢ 2 ) ⁢ and Vh total =Vh protein +Vh salt ; (c3) Vh total — exp =Vh protein — exp +Vh salt — exp (c4) where Vh protein —exp is an electrical energy for enabling protein focusing, to be determined by the equation (c1); Vh salt — exp is an electrical energy for enabling salt electrophoresis, to be determined by the equation (c2); Vh protein — ref is an electrical energy for enabling focusing of a reference protein; Vh salt — ref is an electrical energy for enabling electrophoresis of a reference salt; M ref and M exp are a weight of a reference protein and the weight of the protein sample, respectively; S blank , S ref , and S exp are an electrical conductivity of a gel solution containing no protein, an electrical conductivity of the reference salt, and the electrical conductivity of the salts in the protein sample, respectively; strip length ref and strip length exp are a reference pH-gradient gel strip length and an actual pH-gradient gel strip length, respectively; pH range ref and pH range exp are a reference pH range and an actual pH range, respectively; and Vh total , Vh protein , and Vh salt are the total electrical energy, an electrical energy for enabling protein focusing, and an electrical energy for enabling salt electrophoresis, respectively; and (d) substituting the electrical conductivity of the salts in the protein sample, the weight of the protein sample, the actual pH-gradient gel strip length, and the actual pH range into the set of equations so as to determine the total electrical energy for enabling protein focusing and achieving optimal protein focusing effects.