Patent ID: 8735351

Claim:
A method for the preparation of a TF-bearing microvesicle having pro-coagulant activity comprising: (i). expressing TF or a variant thereof having pro-coagulant activity in an eukaryotic cell selected from a yeast, plant or insect cell; (ii). lysing the TF-expressing cells of step (i) in the absence of detergents or, when detergents are used, using concentrations of said detergents below the critical micellar concentration; (iii). recovering TF-bearing microvesicles from the lysed cells of step (ii) and (iv). contacting the vesicles obtained in step (iii) with a negatively charged phospholipid in the absence of detergents under conditions adequate for the incorporation of said phospholipid into said vesicles; wherein said microvesicles are formed by lipid membranes, or fragments thereof, from said eukaryotic cell.