Patent ID: 7138515

Claim:
A method for synthesizing a heterologous protein or a heterologous polypeptide comprising the steps of providing a cell-free protein expressing system, providing a polynucleotide encoding the heterologous protein or heterologous polypeptide and a polynucleotide that promotes translation activity, wherein the polynucleotide that promotes translation activity has an RNA higher-order structure including PK (pseudoknot) I, II, and III structures and is selected from: 1) one of the base sequences of SEQ ID NOs: 1–7; 2) a base sequence of SEQ ID NO: 1, except that positions 187–188 of the base sequence of SEQ ID NO: 1 are cc instead of uu and positions 159–160 are gg instead of aa; and 3) a base sequence containing the base sequence of 1) or 2); and wherein the polynucleotide encoding the heterologous protein or heterologous polyptptide is immediately downstream from the PKI structure of the polynucleotide that promotes translation activity, translating the polynucleotide encoding the heterologous protein or heterologous polypeptide in the cell-free protein expressing system to form the heterologous protein or heterologous polypeptide, wherein the translating is initiated and/or promoted by the polynucleotide that promotes translation activity, and isolating the heterologous protein or heterologous polypeptide.