Patent ID: 8309343

Claim:
A sedimentation assembly for concentrating cells in a suspension, comprising: a first chamber for receiving a suspension including a cell population, the first chamber including a cell concentration zone for receiving a concentrated population of the cells upon application of a sedimentation force upon the first chamber, said first chamber further comprising a first port in communication with said cell concentration zone; a second chamber including a second port configured to be repeatedly removably placed in fluid communication with the first port of the first chamber; and one of the first and second ports having a resealable closure and the other of the first and second ports comprising a member configured to open said resealable closure when the chambers are connected, the resealable closure being configured to automatically close when the chambers are disconnected; the first and second chambers being configured for placement together in a sedimentation force field as a unit with the first and second chambers in fluid communication, whereby upon application of the sedimentation force field said second chamber receives at least a portion of the suspension including a concentrated population of the cells from the cell concentration zone of said first chamber.