Patent ID: 6846660

Claim:
A process for producing an aldehyde dehydrogenase wherein the dehydrogenase has a molecular weight of 150,000±6,000 Da and comprises two homologous subunits or has a molecular weight of 230,000±9,000 Da and comprises three homologous subunits, each subunit having a molecular weight of about 75,000±3,000 Da; has L-sorbosone, D-glucosone, D-glucose, D-galactose, D-mannose, L-gulose, D-xylose, D-ribose, and D-arabinose dehydrogenase activity; utilizes as cofactors pyrroloquinoline quinone and heme c; has an optimum pH from 7.0 to 8.5; and is inhibited by Co 2+ , Cu 2+ , Fe 2+ , NI 2+ , Zn 2+ , monoiodoacetate and ethylenediamine tetraacetic acid, which process comprises cultivating cells of a Gluconobacter microorganism containing aldehyde dehydrogenase in an aqueous nutrient medium under aerobic conditions; disrupting the cells of the cultivated Glucanobacter microorganism and purifying the aldehyde dehydrogenase from the disrupted cells.