Patent ID: 8287879

Claim:
An immunogenic composition for inducing a protective immune response in a host comprising: a recombinant Bacille Calmette Guerin (rBCG) expressing: (1) at least one Mycobacteria major extracellular protein selected from the group consisting of 23.5 kDa protein, 30 kDa protein, 32A kDa protein, and 32B kDa protein, and combinations thereof; and (2) at least one cytokine selected from the group consisting of interferon gamma, interleukin-4 receptor, interleukin-2, interleukin-12, granulocyte macrophage colony stimulating factor, wherein said at least one Mycobacteria major extracellular protein and said at least one cytokine are expressed under the control of a promoter that is not a heat shock promoter and said at least one Mycobacteria major extracellular protein is over-expressed and secreted; and wherein said at least one Mycobacteria major extracellular protein and said at least one cytokine act synergistically to induce the protective immune response in the host.