Patent ID: 7060484

Claim:
Isolated nucleic acid encoding a novel polypeptide (NP) having the amino acid sequence of reference sequence thrombin of SEQ. ID. NO. 2, in which at least one amino acid residue has been substituted for at least for one of the following thrombin amino acid residues, or at least one of the following thrombin amino acid residues has been deleted, or an amino acid residue has been inserted immediately adjacent to at least one of the following thrombin amino acid residues: R56, S58, Q60, E61, D71, W86, D87, N89, F90, T91, N93, D94, K101, H102, Y107, N110, E118, K119, R129, E130, R134, D149, D158, R159, E160, S164, Q167, K181, N187, K190, S194, E205, K210, D211, S212, T213, D219, D229, K232, A236, N252, N253, W263, E265, R269, D276, G271, K272, Y273, F275, Q287, W285, D291, or Q292, provided, however, that the nucleic acid does not encode thrombin K190A, thrombin K210E, thrombin E61K, thrombin N187D K190E, thrombin desP84P85W86, thrombin in which at least one amino acid residue within the thrombin activation site has been substituted or deleted, or a residue inserted adjacent to such an amino acid residue, or thrombin in which loop F55-E61 is replaced by the equivalent loop from tissue plasminogen activator.