Patent ID: 7871814

Claim:
A live, replication-competent, recombinant, attenuated yellow fever virus (YFV), comprising, in order from 5′ to 3′, a 5′ untranslated region (UTR) from a parent YFV, an open reading frame (ORF) encoding all viral proteins of the parent YFV, an internal ribosome entry site (IRES), an exogenous nucleic acid insert that encodes a polypeptide other than a polypeptide encoded by the parent YFV, and a 3′ UTR from the parent YFV, wherein the recombinant YFV is capable of propagating in a mammalian host, and wherein the parent YFV is strain 17D, and wherein the nucleic acid insert is retained for at least three passages of the recombinant YFV.