Patent ID: 8030084

Claim:
A method for determining the amount of thyroglobulin in a test sample, comprising: (a) digesting thyroglobulin from said test sample to form peptide T129; (b) purifying said peptide T129 from step (a); (c) ionizing said peptide T129 from step (b) to generate a multiply charged ion detectable by mass spectrometry; wherein said ion has a mass/charge ratio of 636.4±0.5; (d) detecting the amount of one or more ions with m/z of 541.3±0.5, 612.3±0.5, 636.4±0.5, 726.4±0.5, 797.4±0.5, 912.4±0.5, and 1059.5±0.5 by mass spectrometry; wherein the amount of said ion(s) detected is related to the amount of said thyroglobulin in said test sample.