Patent ID: 7939292

Claim:
A method of sequencing comprising: contacting a polysaccharide having a disaccharide repeat unit of a uronic acid [α-L-iduronic acid (I) or β-D-glucuronic acid (G)] linked 1,4 to α-D-hexosamine (H) with a protein comprising a modified heparinase III to allow cleavage of the polysaccharide by the modified heparinase III, thereby obtaining fragments of the polysaccharide, wherein the modified heparinase III has the amino acid sequence of the mature peptide of SEQ ID NO: 2, wherein at least one histidine residue selected from the group consisting of His36, His105, His110, His139, His152, His225, His234, His241, His424, His469, and His539 has been substituted with a residue selected from the group consisting of alanine, serine, tyrosine, threonine, and lysine, analyzing the fragments to identify one or more properties thereof, and determining the sequence of the polysaccharide based on the one or more properties.