Patent ID: 7754479

Claim:
A method for a long-term culture for more than 3 months of avian spermatogonial stem cells, which comprises the steps of: (a) preparing an avian testis from an avian aged 2-70 weeks; (b) isolating a population of testicular cells from said avian testis; (c) culturing said population of testicular cells for about 5 to 10 days on plates in a medium containing a cell growth factor to form a colony of spermatogonial stem cells; and (d) taking a colony of spermatogonial stem cells and culturing said avian spermatogonial stem cells for more than about 80 to 85 days on a feeder cell layer in a medium containing a cell growth factor; wherein said step (b) is carried out by treating said avian testis with a mixture of collagenase and trypsin; said medium in steps (c) and (d) includes FBS (fetal bovine serum), avian serum, non-essential amino acids, Hepes buffer, and β-mercaptoethanol; and said feeder cell is avian gonadal stroma cell or testicular stroma cell.