Patent ID: 7817335

Claim:
A microscope apparatus comprising: a light source device including a light intensity adjuster which changes light intensity of illumination light of plural different wavelength regions selectively extracted from light emitted from a light source, wherein the light source device emits the illumination light of the plural different wavelength regions such that a light intensity ratio of light of one wavelength region to light of another wavelength region is variable, a storage table which stores set values of the light intensity adjuster for each of a plurality of combination patterns of the wavelength regions of the illumination light emitted from the light source device, a setting controller which controls the light source device to change a setting of the light intensity adjuster based on the set values stored in the storage table according to wavelength designation information designating one of the combination patterns of the wavelength regions, a reference sample having a predetermined reflectance ratio between two different wavelength regions selectable from the plural different wavelength regions, an illumination unit which irradiates the reference sample with the illumination light of each of the different wavelength regions emitted from the light source device, an image forming unit which forms a reference optical image of the reference sample irradiated with the illumination light, an imager which captures the reference optical image of each of the different wavelength regions to generate a reference observation image, and an image processor which calculates a statistic value of pixel values of the reference observation image for each of the different wavelength regions, wherein the set values stored in the storage table are determined based on the predetermined reflectance ratio and the statistic value for each of the combination patterns of the wavelength regions.