Patent ID: 8247004

Claim:
A method for treating a skin disorder comprising administering a therapeutically effective amount of a composition comprising Plectranthus amboinicus Benth crude extracts or Plectranthus amboinicus Benth extracts and a therapeutically effective amount of Centella asiatica Urban extracts, wherein the Plectranthus amboinicus Benth crude extracts and Plectranthus amboinicus Benth extracts are obtained by a method comprising the steps of: (a) extracting juices from whole Plectranthus amboinicus Benth plants directly with alcohol; (b) concentrating the Plectranthus amboinicus Benth juices to obtain Plectranthus amboinicus Benth crude extracts; (c) eluting the Plectranthus amboinicus Benth crude extracts with solvents by column separation; and (d) partially separating the solvent-eluted Plectranthus amboinicus Benth crude extracts to obtain Plectranthus amboinicus Benth extracts; wherein the Plectranthus amboinicus Benth crude extracts have the following HPLC peak of retention time: Peak Retention time (min) 1 18.7 2 19.8 3 20.7 4 23.9 5 24.2 6 28.3 7 33.2 8 33.7 9 37.5 10 41.7 11 43.4 12 45.8 wherein said HPLC is conducted at the condition of: Flow rate: 1.0 ml/min Pressure Limit: 250 kgf/cm 2 Sample amount: 10 μl PDA condition: Sampling period: 0.64 sec Wavelength range: 190-370 nm Channel: 204, 254 nm Elution Profile: Time (min) Mobile phase 0 10 55 60 H 2 O 90% 90% 20% 80% Acetonitrile 10% 10% 80% 20% and wherein the Plectranthus amboinicus Benth extracts have the following HPLC peak of retention time: Peak Retention time (min) 1 11.1 2 11.4 3 16.9 4 17.3 5 22.9 6 24.1 7 25.1 8 26.6 9 27.7 10 28.5 11 29.3 12 29.6 13 30.4 14 31.3 15 33.0 wherein said HPLC is conducted at the condition of: Flow rate: 1.0 ml/min Pressure Limit: 250 kgf/cm 2 Sample amount: 10 μl PDA conditions: Sampling period: 0.64 sec Wavelength range: 190-370 nm Channels: 270, 320 nm Elution Profile: Time (min) Mobile phase 0 15 45 50 n-hexane 95% 85% 30% 95% Ethyl acetate 5% 15% 70% 5%