Patent ID: 7491532

Claim:
A eukaryotic cell for producing a protein of interest, said eukaryotic cell comprising: a first nucleotide sequence encoding an adenoviral E1A protein; a second nucleotide sequence encoding an adenoviral E1B protein; wherein the genome of the eukaryotic cell lacks a nucleotide sequence encoding a structural adenoviral protein and wherein the eukaryotic cell does not express a structural adenoviral protein; and a recombinant nucleotide sequence in expressible format encoding the protein of interest, wherein the recombinant nucleotide sequence in expressible format encoding the protein of interest is integrated into the genome of the eukaryotic cell, and wherein the eukaryotic cell is a human embryonic retinoblast that has been immortalized by introduction therein of said first and second nucleotide sequences, and wherein the protein of interest is a protein other than an adenoviral protein.