Patent ID: 8557963

Claim:
A process of aqueous protein extraction from Brassicaceae oilseed meal to obtain a napin-rich protein extract, a cruciferin-rich protein extract, and a low-protein residue, the process comprising the steps of: (a) performing aqueous extraction of the Brassicaceae oilseed meal at a temperature of from about 18° C. to about 50° C. using an aqueous solvent at a pH of from 2.5 to 3.5 containing a salt at a concentration of from 0.25% to 2.0% w/v of the aqueous solvent, wherein the Brassicaceae oilseed meal is mixed with the aqueous solvent at a meal-to-aqueous solvent ratio of from about 1:10 to about 1:90, to obtain: (i) a soluble napin-rich protein extract; and (ii) a cruciferin-rich protein residue; and (b) performing aqueous extraction of the cruciferin-rich protein residue from step (a) at a temperature of from about 18° C. to about 50° C. using an aqueous alkali solvent at a pH of from about 7.0 to about 13.0, wherein the cruciferin-rich protein residue is mixed with the aqueous alkali solvent at a residue-to-aqueous alkali solvent ratio of from about 1:10 to about 1:90, to obtain: (i) a soluble cruciferin-rich protein extract; and (ii) a low-protein residue, wherein, steps (a) and (b) are performed sequentially.