Patent ID: 8722354

Claim:
A method of labeling a living cell, the method comprising contacting at least one living cell with a compound of Formula I: wherein: B at each occurrence is independently —CH 2 OC(O)CH 3 or a group of Formula II: Y 1 and Y 2 are independently O or NR 6 R 1 , R 2 , R 3 , R 4 , and R 5 at each occurrence are independently substituted or unsubstituted C1 to C8 alkyl; R 6 at each occurrence is independently H or substituted or unsubstituted C1 to C8 alkyl; R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 are independently H, substituted or unsubstituted C1 to C8 alkyl, substituted or unsubstituted C6 to C14 aryl, substituted or unsubstituted C1 to C8 alkoxy, substituted or unsubstituted C6 to C14 aryloxy, hydroxy, halo, nitro, nitrile, amino, amido, imido, urea, amidine, guanidine, enamine, urethane, oxime, hydroxylamine, carboxyl, ester, oxo, thiol, sulfide, sulfoxide, sulfone, sulfonyl, sulfonate, sulfonamide, hydrazine, hydrazide, hydrazone, azide, cyanate, isocyanate, thiocyanate, or isothiocyanate; and one of R 13 , R 14 , R 15 or R 16 is a group of Formula III: wherein: L is a linker selected from the group consisting of substituted or unsubstituted C1 to C30 alkylene and substituted or unsubstituted C1 to C30 heteroalkylene, or is an amino acid linker comprising from 1 to about 20 amino acids; and P is a polypeptide comprising a C-terminus and an N-terminus, wherein the C-terminus of the polypeptide is bonded to the NH 2 moiety, and wherein P is cleavable by a sortase enzyme; wherein the living cell is a Gram-positive bacterium.