Patent ID: 8067374

Claim:
A method for preparing an esterified atelocollagen for use in a collagen-based adhesive hemostatic agent, comprising: removing immunogenic telo-peptides from porcine collagen through enzyme treatment to afford atelocollagen; reacting an aqueous 0.5-5 wt % solution of the atelocollagen with a 65-95 wt % ethanol solution at 4° C. for 24-48 hours with stirring to afford an esterified atelocollagen solution; and adjusting the pH of the esterified atelocollagen solution to 7.4 with 0.1-1 M acetic acid; wherein the method yields an aqueous solution of 0.5-5 wt % esterified atelocollagen having a pH of 7.4 adjusted with 0.1-1 M acetic acid.