Patent ID: 7067292

Claim:
A method for the production of phospholipids of the general formula (I) in which R 1 and R 2 are independently of one another a saturated, mono- or polysaturated C 10-30 -acyl radical, and R 3 is comprising: forming a reaction mixture comprising a phospholipid mixture with a phospholipase consisting of phospholipase D (PLD), containing no organic solvents, in an aqueous, unbuffered phase and at least one divalent metal ion, wherein at least one phosphatidyl acid (PA) is produced in a first stage of the reaction; isolating the at least one PA produced from the said first stage of the reaction; and reacting the at least one PA isolated from said first stage with PLD, and at least one divalent metal ion, and at least one compound of formula (II) R 3 —H in a second stage of the process, where R 3 is as defined for formula (I) and wherein the said compound of formula (II) is only added in said second stage and is not present in said first stage, to produce at least one phospholipid of formula (I).