Patent ID: 7601529

Claim:
A variant of a parent enzyme having pectate lyase activity (EC 4.2.2.2), which variant comprises an alteration at one or more positions selected from the group consisting of: 105, 139, 146, 213, 218, 258, 331, and 337, wherein (a) the alteration(s) are independently (i) an insertion of an amino acid downstream of the amino acid which occupies the position; (ii) a deletion of the amino acid which occupies the position; or (iii) a substitution of the amino acid which occupies the position with a different amino acid; (b) the variant has an amino acid sequence which is at least 90% identical to SEQ ID NO: 2; (c) the variant has pectate lyase activity; and (d) each position corresponds to a position of the amino acid sequence of SEQ ID NO: 2.