Patent ID: 8148062

Claim:
A method, comprising: a) providing i) nucleic acid comprising a disease-related gene; ii) PCR primers capable of hybridizing to a portion of said disease-related gene so as to create amplified template; iii) transcription-translation reagents capable of producing nascent protein or protein fragments from said amplified template, said nascent protein or protein fragments comprising an affinity marker; iv) an antibody capable of binding to said affinity marker of said nascent protein; b) mixing said nucleic acid and said PCR primers under conditions such that said amplified template is created; c) contacting said amplified template with said transcription-translation reagents under conditions such that nascent protein or protein fragment is produced and said affinity marker is added to said nascent protein or fragment during translation; and d) reacting said antibody with said nascent protein or protein fragment.