Patent ID: 8420833

Claim:
A method of producing a carboxylic acid of Formula (I), wherein R 1 is H or —CH 3 ; R 2 is H, —CH 3 , or —CH 2 CH 3 ; and R 3 is H, —CH 3 , or —CH 2 CH 3 ; or R 2 and R 3 are each H, and R 1 is H, —CH 3 , or —(CH 2 ) n CH 3 , where n is an integer from 1 to 6; and wherein the method comprises culturing a host cell comprising a vector encoding a non-naturally occurring polyketide synthase in a culture medium under conditions where the PKS is expressed and the carboxylic acid is produced, wherein the PKS comprises a loading domain and two or more extender modules, and the PKS is a hybrid PKS comprising a combination of modules which in nature are not found in this combination.