Patent ID: 8476013

Claim:
A method for determining the presence or absence of a fetal aneuploidy comprising: a) digesting nucleic acid from a pregnant female, which nucleic acid comprises fetal nucleic acid and maternal nucleic acid, with a methylation sensitive restriction enzyme that specifically digests non-methylated maternal nucleic acid at a plurality of loci, wherein the plurality of loci comprises the locus of SEQ ID NO:38, thereby enriching the fetal nucleic acid; b) determining the amount of fetal nucleic acid from a target chromosome; c) determining the amount of fetal nucleic acid from a reference chromosome and; d) comparing the amount of fetal nucleic acid determined in step (b) to the amount of fetal nucleic acid determined in step (c), whereby a statistically significant difference between the amount of fetal nucleic acid determined in step (b) and the amount of fetal nucleic acid determined in step (c) determines the presence of a fetal aneuploidy.