Patent ID: 7846673

Claim:
A method of screening for a disease associated with abnormal expression of CA IX in a human subject, wherein said disease associated with abnormal expression of CA IX is a cancerous disease and/or a disease associated with hypoxia, comprising: (a) measuring human soluble CA IX in a type of body fluid sample of said subject; (b) comparing the measured human soluble CA IX in said subject's body fluid sample with that measured in a sample of the same type of body fluid of a human control known to be unaffected with said disease associated with CA IX abnormal expression; and (c) determining that there is an indication that said subject is afflicted with said disease associated with CA IX abnormal expression when there is an elevated level of human soluble CA IX in said subject's body fluid sample relative to said control's body fluid sample; wherein said human soluble CA IX is a protein having a molecular weight of from about 15 kilodaltons (kD) to about 54 kD found in human body fluids which is specifically bound by the V/10 monoclonal antibody that is secreted from the hybridoma V/10-VU, which was deposited under the Budapest Treaty at the 14 International Depository Authority of the Belgian Coordinated Collection of Miccroorganisms (BCCM) at the Laboratorium voor Moleculaire Biologie-Plasmidencollectie (LMBP) at the Universeit Gent in Gent, Belgium, under Accession No. LMBP 6009CB.