Patent ID: 7393656

Claim:
A method of determining a signaling phenotype of a cell population, said method comprising: contacting said cell population with at least two distinct potentiators in separate cultures; determining signaling node states of a plurality of signaling nodes in one or more cells from each of said separate cultures, wherein at least two of said plurality of signaling nodes belong to the same signaling pathway comprising: permeabilizing said one or more cells from each of said separate cultures; contacting said permeabilized cells from each of said separate cultures with at least one detectible state-specific binding element for each of said plurality of signaling nodes, wherein said detectible state-specific binding elements are distinguishably labeled; and detecting the presence or absence of binding of each of said distinguishably labeled state-specific binding elements to said plurality of signaling nodes in each of said permeabilized cells from each of said separate cultures; creating a response panel for said cell population comprising said determined signaling node states; and determining a signaling phenotype of said cell population based on said response panel.