Patent ID: 7250298

Claim:
An isolated polynucleotide encoding a variant polypeptide of wild-type DsRed of SEQ ID NO: 2 or a variant of the rapidly maturing Discosoma red fluorescent protein T4 (DsRed.T4) of SEQ ID NO: 3, the variant polypeptide having reduced oligomerization relative wild-type DsRed, the variant polypeptide comprising the amino acid substitutions K83M, K163H, and Y193H, the variant polypeptide further comprising at least one amino acid substitution selected from the group consisting of E26Y, K92T, V96S, T106E, T108Q, I125K, S131A, I180V, and M182K the variant polypeptide further comprising at least one amino acid substitution selected from the group consisting of R13Q, R36K, K47Q, M141A, and I210V.