Patent ID: 7276348

Claim:
A method of identifying non-toxic F 1 F 0 -ATPase inhibitors, comprising: a) providing: i) a sample comprising mitochondrial F 1 F 0 -ATPase, and ii) a candidate non-toxic F 1 F 0 -ATPase inhibitor; and b) contacting said candidate non-toxic F 1 F 0 -ATPase inhibitor with said sample; c) measuring the kcat/Km of said mitochondrial F 1 F 0 -ATPase; and d) identifying said candidate non-toxic F 1 F 0 -ATPase inhibitors that bind predominantly a F 1 F 0 -ATPase-substrate complex and that do not alter said kcat/Km ratio of said mitochondrial F 1 F 0 -ATPase upon binding of said mitochondrial F 1 F 0 -ATPase as non-toxic F 1 F 0 -ATPase inhibitors.