Patent ID: 6977150

Claim:
A reconstituted cell-free translation system, which lacks more than one active wild-type elongator amino acyl tRNA species, for generating a peptidomimetic product comprising: (a) purified translation factors; and (b) more than one elongator tRNA species which (i) is charged with a non-naturally occurring amino acid or amino acid analog, and (ii) recognizes a trinucleotide sense codon, wherein the elongator tRNA species charged with a non-naturally occurring amino acid or amino acid analog replaces the wild-type elongator amino acyl tRNA species, wherein the cell-free translation system translates exogenously added mRNA species with highly selective incorporation at each of said trinucleotide sense codons to form the peptidomimetic product, wherein the peptidomimetic product comprises said non-naturally occurring amino acids or amino acid analogs, and wherein the system lacks the ability to synthesize the wild-type amino acyl tRNA species.