Patent ID: 8357504

Claim:
Method for quantitative microbiological determination of individual vitamins, amino acids, or other substances to be tested that are necessary for life in a substance mixture comprising: providing a microtitration plate for microbiological growth and metabolism determination having wells, wherein the respective wells are provided with a predetermined number of viable cells of a suitable microorganism which provide a reproducible growth curve, and wherein the microorganism is in a freeze dried condition in a freezing and storage medium comprising trehalose, sucrose or a mixture of trehalose and sucrose, and which medium is deficient in the substance to be tested, adding water and a substance to be tested comprising an individual vitamin, amino acid, or other substance to be tested that is necessary for life, and determining quantitatively the levels of vitamin, amino acid, or other substance to be tested that is necessary for life in the test substance by measuring microorganism growth against at least one standard solution of the vitamin, amino acid, or other substance to be tested.