Patent ID: 8614069

Claim:
A polypeptide, comprising: a) a non-cytotoxic protease selected from the group consisting of Clostridial neurotoxin protease and Neisseria IgA protease, wherein the non-cytotoxic protease is capable of cleaving a SNARE, protein; b) a translocation peptide that is capable of translocating said non-cytotoxic protease from within an endosome of a mammalian cell, across the endosomal membrane thereof and into the cytosol of the mammalian cell, wherein the translocation peptide is selected from the group consisting of: a clostridial neurotoxin translocation domain selected from the group consisting of Botulinum Type A, B, C, D, F, F and G neurotoxin translocation domain and tetanus neurotoxin translocation domain, diphtheria toxin translocation domain, Pseudomonas exotoxin type A translocation domain, Anthrax toxin translocation domain, Influenza Virus haemagglutinin translocation domain, Semliki Forest Virus fusogenic protein translocation domain, Semliki Forest Virus E1 protein translocation domain, Vesicular Stomatitis Virus glycoprotein G translocation domain, SER Virus F protein translocation domain, and Foamy Virus envelope glycoprotein translocation domain; and c) an epidermal growth factor (EGF) mutein, wherein said EGF mutein comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 11, and wherein said EGF mutein retains the binding ability of SEQ ID NO: 11 to bind to an EGF receptor.