Patent ID: 8357403

Claim:
A method for preparing a tissue graft, comprising the steps: (a) processing a placenta comprising an amnion membrane layer and a chorion tissue layer to separate the chorion tissue layer from the amnion layer, wherein the amnion membrane layer comprises a first side comprising epithelial cells adjacent to a basement membrane and a second side comprising a jelly-like fibroblast cellular layer; (b) removing substantially all of the epithelial cells to expose the basement membrane of the amnion membrane layer while maintaining the jelly-like fibroblast cellular layer to produce a first membrane; (c) laminating a second amnion membrane layer onto the first amnion membrane layer, wherein the epithelial cells have not been removed from the first side of the second amnion membrane, and wherein the fibroblast layer of the first amnion membrane layer is adjacent to the epithelial layer of the second amnion membrane layer; and (d) optionally laminating one or more additional membranes on the first or second amnion membrane layers to produce a layered tissue graft.