Patent ID: 8765934

Claim:
A method for conjugating a nucleic acid with a molecule, comprising steps of: (a) reacting the nucleic acid having a 5′-monophosphate with an activating agent in a first buffer to form a solution; (b) mixing an alcohol with the solution formed in the step (a) to obtain an intermediate; and (c) carrying out one of steps (c1) to (c2) (c1) dissolving the intermediate in a second buffer containing an ethylenediaminetetraacetic acid (EDTA) and adding a first nucleophile thereinto to react the intermediate with the first nucleophile, wherein the first nucleophile includes the molecule; and (c2) providing the molecule, dissolving the intermediate in the second buffer containing the EDTA and adding a second nucleophile thereinto to react the intermediate with the second nucleophile, and then reacting the second nucleophile with the molecule.