Patent ID: 8637253

Claim:
A method, comprising: (a) contacting UvsX, UvsY, and Rb69 gp32 proteins with first and second single stranded nucleic acid primers specific for a double stranded target nucleic acid molecule to provide first and second nucleoprotein primers, the UvsX and UvsY proteins each being derived from a myoviridae phage; (b) contacting the first nucleoprotein primer with the double stranded target nucleic acid molecule to create a first D loop structure at a first portion of the double stranded target nucleic acid molecule; (c) contacting the second nucleoprotein primer with the double stranded target nucleic acid molecule to create a second D loop structure at a second portion of the double stranded target nucleic acid molecule so that the 3′ ends of the first and second nucleic acid primers are oriented toward each other on the same double stranded target nucleic acid molecule without completely denaturing the target nucleic acid molecule; and (d) extending the 3′ end of the first and second nucleoprotein primers with one or more polymerases capable of strand displacement synthesis and dNTPs to provide first and second double stranded target nucleic acid molecules and first and second displaced strands of nucleic acid.