Patent ID: 7767419

Claim:
A stepwise method for expression of NT-BNP (N-terminus brain natriuretic peptide) in Escherichia coli ( E. coli ) without an N-terminal histidine affinity chromatography tag for purification comprising sequentially: a) introducing SEQ ID NO:1 at the 5′ end of a nucleic acid encoding for NT-BNP; b) replacing codons 3 through 12 of said nucleic acid encoding for NT-BNP of step a with SEQ ID NO:2; c) deleting codons 1 and 2 of said nucleic acid encoding for NT-BNP of step b; and d) transfecting an E. coli culture with the nucleic acid of step c wherein said E. coli culture then expresses NT-BNP without said affinity chromatography based tag for purification.