Patent ID: 8597875

Claim:
A method for diagnosing liver toxicity comprising: (a) determining the amount of at least five of the following analytes 4-Hydroxyphenylpyruvic acid, Palmitic acid (C16:0), Linoleic acid (C18:cis[9,12]2), Arachidonic acid (C20:cis-[5,8,11,14]4), Docosahexaenoic acid (C22:cis[4,7,10,13,16,19]6), alpha-Tocopherol, Cholesterol, Glycerolphosphate, Lignoceric acid (C24:0), Heptadecanoic acid (C17:0), Tricosanoic acid (C23:0), myo-Inositol-2-monophosphate, Behenic acid (C22:0), 4-Hydroxysphinganine, Nervonic acid (C24:1), Eicosatrienoic acid (C20:3), Glycerol-3-Phosphate, Phosphatidylcholine (C18:2, C20:4), Ceramide (d18:1, C24:1), Ceramide (d18:2, C24:0), Sphingomyelin (d18:1, C16:0), 1,2-Dioleoyl-sn-glycero-3-phosphatidyl-L-serine, Phosphatidylcholine (C18:0/C22:6), 18:1 Lyso PC, or Phosphatidylcholine in a test bodyfluid sample of a male subject suspected to suffer from liver toxicity, or determining the amount of at least five of the following analytes Glycerol-3-Phosphate, Coenzyme Q9, Glucose, Glycerol, Palmitic acid (C16:0), Stearic acid (C18:0), Arachidonic acid (C20:cis-[5,8,11,14]4), Docosahexaenoic acid (C22:cis[4,7,10,13,16,19]6), Cholesterol, Glycerolphosphate, Lignoceric acid (C24:0), Heptadecanoic acid (C17:0), Eicosanoic acid (C20:0), Tricosanoic acid (C23:0), Phosphate, Behenic acid (C22:0), 4-Hydroxysphinganine, Nervonic acid (C24:1), 14-Methylhexadecanoic acid, Eicosatrienoic acid (C20:3), a lipid molecule having an apparent m/z ratio of 91 Da as determined by GC MS single ion monitoring at a chromatographic retention time of 17.39 minutes being obtainable from a rat lipid plasma fraction, 5-Methoxysphingosine, erythro-sphingosine, Threonine, Ceramide (d18:1, C24:1), Diacylglycerides (DAG) (C18:1, C18:2), Triacylglyceride (TAG), 1,2-Dioleoyl-sn-glycero-3-phosphatidyl-L-serine, Linoleic acid (C18:cis[9,12]2), or Phosphatidylcholine in a test bodyfluid sample of a female subject suspected to suffer from liver toxicity; (b) comparing the amounts determined in step (a) to a reference; and, thereby, (c) diagnosing liver toxicity.