Patent ID: 8921042

Claim:
A method of identifying a compound as an inhibitor of Parkin Interacting Substrate (PARIS) from screening compounds, the method comprising: a) exposing a cell line containing a reporter construct to the compounds to identify an inducer of peroxisome profilerator-activated receptor gamma coactivator -1α(PGC-1α); wherein the reporter construct comprises a cell line that stably expresses pGL3-h PGC-1α wherein a PGC-1α promoter is operably linked to a reporter gene; and wherein the inducer of PGC-1α stimulates the PGC-1α promoter to express the reporter gene; b) exposing the cell line to the inducer of PGC-1α identified in step a) in the presence of PARIS to identify a compound that maintains its ability to stimulate the PGC-1αpromoter to express the reporter gene in the presence of PARIS, wherein the compound thereby inhibits the ability of PARIS to inhibit the PGC-1α promoter activity to identify an inhibitor of PARIS.