Patent ID: 7524654

Claim:
An isolated cell that comprises a polynucleotide that is operably linked to a regulatory nucleic acid, wherein the polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 9, and a sequence that is capable of hybridizing to the full-length complement of SEQ ID NO: 7 or 9 under high stringency conditions, wherein the conditions comprise hybridization at 65° C. in 1% BSA, 1mM EDTA, 0.5M NaHPO 4 (pH 7.2), 7% SDS, and washing at 68° C. in 0.2×SSC, 0.1% SDS, and wherein the nucleotide sequence encodes an amino acid sequence having one or more of the following features: (a) catalyses the conversion of sucrose into isomaltulose and concomitantly produces trehalulose at a yield of less than 5% of the yield of isomaltulose; or (b) catalyses the conversion of sucrose into isomaltulose with a Km of less than about 50 mM and with and V max of at least about 400 μmoles isomaltulose/mg protein/min; or (c) catalyses the conversion of sucrose into isomaltulose but not the hydrolysis of isomaltulose.