Patent ID: 7778779

Claim:
A method of identifying, screening, characterizing or designing a chemical entity that potentially binds a FIH (Factor Inhibiting HIF (Hypoxia Inducible Factor)), comprising: (a) generating a three-dimensional model of FIH on a computer wherein the three-dimensional model has the x-ray structural coordinates as set forth in the structure 1, 2 or 3 in Table 3; (b) providing a structural model of a chemical entity on the computer; (c) identifying the amino acid residues forming a binding site of the FIH from the three-dimensional model in step (a) in order to generate a three-dimensional representation of the binding site of the FIH, wherein the binding site of the FIH comprises amino acids Tyr145, Leu186, Leu188, Thr196, Phe207, Lys214 and Ile281 as set forth in SEQ ID NO: 30, wherein the amino acids have the structural coordinates as shown in the structure 1, 2 or 3 in Table 3; (d) employing the structural model from step (b) and the three-dimensional representation from step (c) to identify a chemical entity that potentially binds with the binding site of the FIH; and (e) obtaining the chemical entity.