Patent ID: 7625750

Claim:
A plurality of isolated deoxyribonucleic acid (DNA) molecules, wherein each DNA molecule of the plurality is immobilized on a solid support, and wherein each DNA molecule of the plurality comprises an expressible template nucleotide sequence of at least about 16 nucleotides encoding an intermediate small interfering ribonucleic acid (RNA) molecule (siRNA) and at least one transcriptional terminator comprising the sequence as set forth in SEQ ID NO:50 or SEQ ID NO:51, which siRNA mediates RNA interference of a target RNA, said intermediate siRNA comprising: a) a 5′ portion, which comprises at least about 15 nucleotides complementary to a sense strand of the target RNA, and a 3′ terminal portion, comprising about 1 to 5 nucleotides that are not complementary to the sense strand of the target RNA, wherein the siRNA selectively hybridizes to the sense strand of the target RNA; or b) a 5′ portion, which comprises at least about 15 nucleotides complementary to an antisense strand of the target RNA, and a 3′ terminal portion, comprising about 1 to 5 nucleotides that are not complementary to the antisense strand of the target RNA, wherein the siRNA selectively hybridizes to the antisense strand of the target RNA; and wherein each DNA molecule of the plurality further comprises an RNA polymerase III (pol III) promoter operatively linked to an enhancer, a distal sequence element (DSE), wherein the enhancer and the DSE are upstream of the expressible template nucleotide sequence, wherein the DSE is about −79 to −72 nucleotides upstream of the expressible template sequence, and wherein the expressible template nucleotide sequence is heterologous with respect to the pol III promoter.