Patent ID: 8574844

Claim:
A reverse transcription polymerase chain reaction (RT-PCR) mixture comprising: a) an oligonucleotide probe and primers specific for Norovirus Genogroup I; b) an oligonucleotide probe and primers specific for Norovirus Genogroup II; c) a universal internal nucleic acid molecule, wherein said molecule contains at least one forward primer annealing site, at least one reverse primer annealing site, and at least one amplifiable region; d) a universal internal control oligonucleotide probe; and e) a universal internal RNA control primer, wherein i) the universal internal control oligonucleotide probe comprises SEQ ID NO:13; or ii) the universal internal RNA control primer is selected from the group consisting of SEQ ID NO:11, SEQ ID NO:12 and combination thereof; or iii) both (i) and (ii).