Patent ID: 8580517

Claim:
A functional G protein biosensor comprising a mammalian α subunit comprising a first amino acid sequence having at least one of a first fluorescent or luminescent protein, and a mammalian βγ subunit complex, wherein the β subunit comprises a second amino acid sequence having at least one of a second fluorescent or luminescent protein and the γ subunit comprises a third amino acid sequence having at least one of a third fluorescent or luminescent protein, wherein said first, second and third fluorescent or luminescent proteins are at least FRET (Fluorescence Resonance Energy Transfer) or BRET (Bioluminescence Resonance Energy Transfer) capable, wherein the first fluorescent or luminescent protein is different from the second and the third fluorescent or luminescent proteins, and wherein the second fluorescent or luminescent protein is the same as the third fluorescent or luminescent protein.