Patent ID: 7229627

Claim:
A method of producing aseptic sporozoite-stage Plasmodium parasites comprising: a) providing a mosquito rearing chamber with solid walls and airtight seals, said walls enclosing a sterilized interior space and adapted for filtered air passage, said chamber separable into a first sub-chamber and a second sub-chamber adapted to receive a mosquito blood feeding station by which mosquitoes within said sub-chamber can feed; b) aseptically transferring sterilized Anopheles -species eggs into said first sub-chamber and providing an aseptic larval rearing broth sufficient for the development of said eggs; c) aseptically transferring said Anopheles -species to said second sub-chamber; d) providing said blood feeding station with an aseptic blood meal, said blood meal comprising gametocyte-stage Plasmodium parasites such that said mosquitoes consume said blood meal and become infected with said Plasmodium parasites thereby; and, e) dissecting said mosquitoes to remove and pool sporozoite-containing salivary glands.