Patent ID: 7635475

Claim:
A humanized diabody-type bispecific antibody selected from the group consisting of: (A) a first single-chain polypeptide comprising a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:43, and a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:46, and a second single-chain polypeptide comprising a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:44, and a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:45, B) a first single-chain polypeptide comprising a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:43 and a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:46, and a second single-chain polypeptide comprising a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:44 and a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:45, wherein Met at position 48 and Ala at position 93 of the heavy chain sequence according to SEQ ID NO:45 are replaced by Ile and Thr, respectively, (C) a first single-chain polypeptide comprising a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:43 and a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:46, and a second single-chain polypeptide comprising a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:44 and a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:45, wherein Arg at position 66, Met at position 69, Arg at position 71 and Thr at position 73 of the heavy chain sequence according to SEQ ID NO:45 are replaced by Lys, Leu, Val and Arg, respectively, and (D) a first single-chain polypeptide comprising a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:43 and a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:46, and a second single-chain polypeptide comprising a light chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:44 and a heavy chain comprising a variable region comprising the amino acid sequence according to SEQ ID NO:45, wherein Met at position 48, Arg at position 66, Met at position 69, Arg at position 71, Thr at position 73 and Ala at position 93 of the heavy chain sequence according to SEQ ID NO:45 are replaced by Ile, Lys, Leu, Val, Arg and Thr, respectively.