Patent ID: 8058509

Claim:
A method for producing an inverted repeat in a plant or plant part comprising a) introducing into the genome of the plant or the plant part a polynucleotide comprising an inverted repeat precursor cassette, wherein said inverted repeat precursor cassette comprises a first DNA segment and a target site, wherein said target site is flanked by a first and a second functional recombination site, wherein said first and second recombination sites are dissimilar and non-recombinogenic with respect to one another; b) introducing into the plant or plant part a second polynucleotide comprising a second DNA segment having sufficient sequence complementarity to the first DNA segment to form, upon transcription of a functional inverted repeat expression unit, an inhibitory hairpin RNA polynucleotide , and said second DNA segment is flanked by said first and said second recombination sites, such that recombination inserts said second DNA segment into said target site in the opposite orientation to said first DNA segment; and, c) introducing into said plant or plant part a recombinase, wherein said recombinase mediates a recombination event in said inverted repeat precursor cassette and thereby produces the functional inverted repeat expression unit.