Patent ID: 8237015

Claim:
A method of producing a transgenic hybrid plant having no or reduced sexual reproduction, the method comprising: (a) stably transforming a first plant with a first nucleic acid construct comprising: (i) a promoter, P1; (ii) a site specific recombinase, RS1; (iii) a promoter, P2; (iv) a selectable marker, SM1; (v) a promoter P3; (vi) at least one nucleotide sequence the expression of which results in no or reduced sexual reproduction, NRSR; and (vii) at least two target sites, TRS2, specific for a site specific recombinase, RS2 that is different from the site specific recombinase, RS1, and further wherein P1 is operably located upstream of RS1, P2 is operably located upstream of SM1 and downstream of RS1, P3 is operably located upstream of NRSR and downstream of SM1, a first TRS2 is located immediately downstream of P1 and a second TRS2 is located upstream of NRSR and downstream of P3; (b) stably transforming a second plant with a second nucleic acid construct comprising: (i) a promoter, P4; (ii) a site specific recombinase, RS2, that is different from the site specific recombinase, RS1, of (a); (iii) a selectable marker, SM2; (iv) at least one nucleotide sequence of interest, NOI; (v) at least two target sites, TRS1, specific for the site specific recombinase, RS1; and (vi) at least two target sites, TRS2, specific for the site specific recombinase, RS2, and further wherein P4 is operably located upstream of SM2, RS2 is located downstream of SM2, a first TRS1 is located immediately downstream of P4, a second TRS1 is located immediately downstream of SM2, a first TRS2 is located immediately upstream of RS2 and downstream of the second TRS1, a second TRS2 is located immediately downstream of RS2 and the NOI is located immediately downstream of the second TRS2; and (c) cross-pollinating the stably transformed first plant of (a) with the stably transformed second plant of (b) to produce a transgenic hybrid plant having no or reduced sexual reproduction.