Patent ID: 8017744

Claim:
A method of coupling an oligonucleotide to a purification support, wherein the support is a functionalized chromatographic support that comprises residues, comprising: a) activating the residues of the support, and b) contacting the activated residues of the support with the oligonucleotide to obtain covalent coupling directly between the oligonucleotide and the residues of the purification support; wherein the oligonucleotide comprises a pyrimidine-rich sequence which forms a triple helix with a double-stranded DNA by hybridizing with a nucleotide sequence in the double-stranded DNA, wherein the support comprises a resin comprising hydroxyl residues activated by esterification with N-hydroxylsuccinimide, and wherein contacting the activated residues with the oligonucleotide creates a covalent amide coupling between the oligonucleotide and the residues of the support.