Patent ID: 8071177

Claim:
A method for producing a substrate, the substrate comprising a base material, a self assembling monolayer formed by self assembling molecules bound onto a surface of the base material, and chelators bound onto the self assembling monolayer, the method comprising the steps of: bonding the self assembling monolayer formed by the self assembling molecules onto the surface of the base material; and bonding the chelators onto the self assembling monolayer, the bonding of the chelators onto the self assembling monolayer being performed within an organic solvent and in the presence of a base, the chelators being bound to the self assembling monolayer at a density within a range of from 0.4/nm 2 to 4/nm 2 and the substrate comprising a non specific adsorption preventing layer having a density greater than 1.6/nm 2 , wherein the self assembling molecules have at one end a functional group selected from the group consisting of â€”SH (thiol), â€”SS (sulfide), â€”SeH (selenol), â€”SeSe (diselenide), and â€”COSH (thioic acid).