Patent ID: 8105770

Claim:
A method for detecting amplification or deletion in a test genomic DNA fragment, which comprises: (a) labeling a test genomic DNA fragment which is a genomic DNA fragment derived from the objective cell to be inspected with either one of a labeling substance of formula (1) or a labeling substance of formula (2), and labeling a control genomic DNA fragment which is used as a standard for detecting difference from said test genomic DNA fragment with the other labeling substance, wherein R 1 -R 4 and R 8 -R 11 represent independently from each other a hydrogen atom or —SO 3 R 15 , wherein R 15 represents a hydrogen atom, an alkali metal atom, an organic ammonium ion or an ammonium ion, R 5 and R 12 represent independently from each other an alkyl group or a sulfoalkyl group, R 6 , R 7 , and R 14 represent independently from each other an alkyl group, and R 13 represents a carboxyalkyl group, wherein R 21 -R 24 and R 28 -R 31 represent independently from each other a hydrogen atom or —SO 3 R 35 , wherein R 35 represents a hydrogen atom, an alkali metal atom, an organic ammonium ion or an ammonium ion, R 25 and R 32 represent independently from each other an alkyl group or a sulfoalkyl group, R 26 , R 27 and R 34 represent independently from each other an alkyl group, and R 33 represents a carboxyalkyl group; (b) competitively hybridizing the labeled test genomic DNA fragment and the labeled control genomic DNA fragment with a sample nucleic acid comprising a nucleotide sequence for detecting difference between the test genomic DNA fragment and the control genomic DNA fragment; and (c) detecting amplification or deletion in the test genomic DNA fragment by using the obtained fluorescence intensity as an indicator.