Patent ID: 7465583

Claim:
A method of administering a nucleic acid to a mammalian subject comprising administering to the mammalian subject a cell that has been contacted with a parvovirus particle comprising a vector genome under conditions sufficient for the parvovirus particle vector genome to enter the cell, wherein the parvovirus particle comprises: a parvovirus capsid; and the vector genome comprising in the 5′ to 3′ direction: (i) a 5′ parvovirus terminal repeat; (ii) a first heterologous nucleotide sequence; (iii) a modified parvovirus terminal repeat such that resolution by Rep protein is reduced; (iv) a separate heterologous nucleotide sequence; and (v) a 3′ parvovirus terminal repeat, wherein the first and second heterologous nucleotide sequences are essentially self-complementary and may form a hairpin structure that comprises the nucleic acid.