Patent ID: 8669059

Claim:
A method of determining the plasmic state in a non-vascular photosynthetic organism that has been transformed with an exogenous sequence comprising: providing in a mixture for a polymerase chain reaction (PCR) a first primer pair, wherein a first primer of said first primer pair anneals to a selected site in the chloroplast genome of said organism, a second primer of said first primer pair anneals to a flanking region of said selected site, and said first primer pair is capable of amplifying a first endogenous chloroplast sequence, a second primer pair wherein a first primer of said second primer pair and a second primer of said second primer pair anneal to a second endogenous chloroplast nucleic acid sequence that is not said first endogenous chloroplast sequence, a polymerase, and a chloroplast nucleic acid molecule from said organism; performing a polymerase chain reaction (PCR); and analyzing the product of said reaction, wherein said selected site is deleted or disrupted when an exogenous nucleic acid is incorporated into said non-vascular photosynthetic organism.