Patent ID: 7306940

Claim:
An electroporation device comprising a signal generator comprising a pulse generator having at least two output electrodes fittable to a substrate comprising cells; said electrodes producing, in said substrate, an electric field which induces permeabilization of the membranes of said cells to facilitate introduction of substances into the cells; the device further comprising a measuring system and an electronic control unit, which constitute a regulator for controlling characteristic quantities of the signal (I 2 ) generated by the signal generator and applied to the substrate on the basis of the impedance (Z(ω)) measured in the substrate; said impedance (Z(ω)) being a function of the degree of permeabilization attained by the membranes of said cells, characterized in that said signal generator generates: a number of first pulses (I 1 ) applied to the electrodes; each first pulse having a constant amplitude; at least one second pulse ( 12 ; Iu 2 ) applied to the electrodes; said second pulse ( 12 ; Iu 2 ) having an amplitude which is a function of the impedance Z(ω) measured in the substrate; at least one third pulse (I 3 ) applied to the electrodes; said third pulse (I 3 ) having a lower amplitude than the first pulses (I 1 ) and the second pulse (I 2 ; Iu 2 ).