Patent ID: 7291478

Claim:
A method of determining the presence of a compound of Formula (I) wherein R 1 and R 2 are independently selected from a group consisting of organic moieties, and X is a leaving group which is energetically labile as an anion or a negatively charged group, with the proviso that when R 2 is methyl and X is fluorine, then R 1 is not isopropyl, cyclohexyl or pinacolyl; when R 2 is methyl and X is S-(2-diisopropylaminoethyl), then R 1 is not ethyl; and when R 2 is methyl and X is S-(2-diethylaminoethyl), then R 1 is not i-butyl, in a sample suspected of containing the same, said method comprising the steps of: treating the sample with a first hydrolyzing agent, wherein said first hydrolyzing agent is selected from a group consisting of an alkaline compound, a tri-substituted organophosphorus hydrolyzing enzymatic agent and combinations thereof, in amounts sufficient to convert the compound of Formula (I) to a detectable amount of corresponding compound of Formula (II) treating the sample with a second hydrolyzing agent, wherein the second hydrolyzing agent is a di-substituted organophosphorus hydrolyzing enzymatic agent, in amounts sufficient to convert the compound of Formula (II) to a detectable amount of corresponding compound of Formula (III) analyzing the sample to determine the presence of the compound of Formula (III) wherein the presence of the compound of Formula (III) indicates the presence of the compound of Formula (I) in the sample.