Patent ID: 8497064

Claim:
A nucleic acid labeling compound represented by the structure wherein: A is hydrogen, monophosphate, diphosphate, triphosphate, α-thiotriphosphate, phosphoramidite, or H-phosphonate; X 1 is O, S, NR 1 , or CHR 2 , wherein R 1 and R 2 are, independently, hydrogen, alkyl, or aryl; R 15 is hydrogen, alkyl, or aryl; Y 1 is hydrogen, N 3 , F, OR 9 , SR 9 , or NHR 9 , wherein R 9 is hydrogen, alkyl, or aryl; Z 1 is hydrogen, N 3 , F, OR 9 , SR 9 , or NHR 10 , wherein R 10 is hydrogen, alkyl, or aryl; one of Y 1 or Z 1 is a group having the formula —X 2 L-M-Q; X 2 is O, S, or NR 16 , wherein R 16 is hydrogen, alkyl, or aryl; L is a linker moiety that is sterically and electronically suitable for incorporation into a nucleic acid; Q is a detectable moiety selected from the group consisting of biotin, carboxyfluorescein, colloidal gold, radiolabels, magnetic particles, fluorescent labels, chemiluminescent or bioluminescent labels, spin labels, dyes, and colored beads; and M is a connecting group comprising a structure that does not interfere with the nucleic acid labeling compound's function.