Patent ID: 8895243

Claim:
A method of determining a colon cancer tissue in a human test patient comprising: obtaining a first sample from a non-cancerous human subject and a second sample from the test patient, measuring a transcription product of the human patched homolog 1 (PTCH1) gene comprising SEQ ID NO: 1 in the first and second sample by contacting the samples with a probe or primer that hybridizes to the splice junction in SEQ ID NO: 1 or the complement of SEQ ID NO: 1, wherein the splice junction is between exon1B and exon 3 of PTCH1, and comparing the level of measured transcription product from the first and second samples, wherein if the level of measured transcription product in the second sample is higher than the level of measured transcription product in the first sample, the test patient comprises a colon cancer tissue.