Patent ID: 7892830

Claim:
A method of creating a clonal culture of human pluripotent stem cells comprising the steps of: providing a culture of human pluripotent stem cells in culture in a fibroblast growth factor-containing culture medium in a culture vessel; selecting a single pluripotent stem cell from the stem cells in culture; initiating a new culture of pluripotent stem cells with the single pluripotent stem cell in a culture vessel containing an extracellular matrix coating a surface inside the culture vessel and a fibroblast growth factor-containing culture medium, the culture medium including an agent selected to increase the cloning efficiency of the pluripotent stem cell culture relative to a pluripotent stem cell culture that does not contain the agent, the agent being a small molecule having the formula: wherein R 1 and R 2 can form a cyclic group, or R 1 is H or an alkyl, and R 2 is H or an alkyl, and R 1 is not the same as R 2 unless R 1 and R 2 are part of a cyclic group; and wherein R 3 and R 4 are attached to any atom in the isoquinoline and are a hydrogen or an alkyl; and culturing the single pluripotent stem cell to create a clonal culture of human pluripotent stem cells.