Patent ID: 7179590

Claim:
A method for reverse transcribing an RNA, that comprises: (a) providing a reverse transcription reaction mixture comprising said RNA, a primer, Mg+2, and a mutant thermoactive DNA polymerase, wherein said mutant DNA polymerase is characterized in that i) in its native form said DNA polymerase comprises a polymerase domain comprising an amino acid sequence that is SEQ ID NO:1, wherein said polymerase domain has the ability to incorporate nucleotides; ii) the amino acid at position 2 of said amino acid sequence is S or A and the amino acid at position 5 of said amino acid sequence is L or I; and iii) the amino acid at position 4 of said amino acid sequence is mutated in comparison to said native sequence to an amino acid other than E, A, G, or P; and (b) treating said reaction mixture at a temperature sufficient for said mutant DNA polymerase to initiate synthesis of an extension product of said primer to provide a cDNA molecule complementary to said RNA.