Patent ID: 8546071

Claim:
A method of detecting a quorum sensing signal in a bacteria sample, the method comprising: adding a detecting substrate and the cell-lysate extract solution to a well of a microtiter plate to form an assay reagent configured to report the detection of the quorum sensing signal, wherein the detecting substrate is an absorbance-based substrate or a luminescence-based substrate, and wherein the cell-lysate extract solution is formed from Agrobacterium tumefaciens NTL4 (pCF218)(pCF372); adding the bacteria sample to the well; incubating the bacteria sample within the well in the presence of the cell-lysate extract solution; measuring absorbance or luminescence of the assay reagent in the presence of the bacteria sample within the well; and determining the presence or absence of quorum sensing signals in the bacteria sample, wherein the quorum sensing signal comprises an acyl-homoserine lactone.