Patent ID: 8617888

Claim:
A method of delivering one or more nuclear proteins into one or more isolated cells, comprising: incubating the cell or cells with a Pseudomonas bacteria deletion mutant lacking genes encoding ExoS, ExoT, and ExoY proteins, said deletion mutant comprising a plasmid or plasmids comprising nucleic acid sequences encoding one or more fusion proteins, wherein each nucleic acid sequence comprises a nucleic acid sequence encoding a nuclear protein fused to a nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein, and the nucleic acid further comprises a nucleic acid sequence encoding a nuclear localization sequence between the nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein and the nucleic acid sequence encoding the nuclear protein; and incubating the isolated infected cell or cells for a period of time sufficient to deliver the one or more nuclear proteins into said cell or cells.