Patent ID: 7319001

Claim:
A method for preparing expressive circular recombinant viral vectors, comprising: i) providing a plurality of linearized viral DNA fragments wherein each fragment contains at least two site-specific recombination sites and at least one restriction enzyme recognition site; ii) providing a plurality of viral vector carriers wherein each viral vector carrier contains a gene cassette having a desired genomic material with a desired nucleic acid sequence flanked with at least two site-specific sites for homologous recombination with the linearized DNA fragments at the two site-specific recombination sites; and iii) reacting the linearized viral DNA fragments and the viral vector carriers to create homologous recombinations in vitro wherein a plurality of recombinant viral vectors carrying the desired genomic material is formed in vitro therefrom; iv) wherein non-recombined linearized viral DNA fragments, non-recombined viral vector carriers, and non-linearized viral DNA do not express a gene for a protein responsible for viral replication and viral particle formation.