Patent ID: 7364853

Claim:
A method of detecting microsatellite instability in a DNA sample comprising: a) co-amplifying a set of microsatellite loci of the DNA sample to produce amplified DNA fragments, the set of loci comprising at least one tetranucleotide repeat locus selected from the group consisting of D1S518, D1S547, D1S1677, D2S1790, D3S2432, D5S2849, D6S1053, D7S3046, D7S1808, D7S3070, D9S2169, D10S1426, D10S2470, D12S391, and D17S1294 wherein the DNA sample is from tumor cells, from cells suspected of being cancerous, or from other biological material suspected of being cancerous; b) determining the size of the amplified DNA fragments; and c) comparing the size of the amplified DNA fragments of step b) to the size of corresponding amplified DNA fragments from a paired normal DNA sample, a difference in size between one or more DNA fragments between the samples being indicative of microsatellite instability.