Patent ID: 8426164

Claim:
A host cell for the production of a desired polypeptide, wherein the host cell comprises: (a) a first nucleic acid sequence that encodes a polypeptide having an amino acid sequence having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:49, wherein the nucleic acid sequence is altered such that the host cell produces less protease compared to a similar cell with a nucleic acid sequence that encodes a polypeptide having an amino acid sequence of SEQ ID NO:49 when cultured under the same conditions, and (b) an inducible promoter operably linked to the nucleic acid sequence, and a promoter sequence that binds a transcriptional activator encoded by the nucleic acid sequence, operably linked to a nucleic acid sequence encoding the desired polypeptide, wherein the inducible promoter and promoter sequence can be induced simultaneously or sequentially.