Patent ID: 7943358

Claim:
A composition comprising: (a) Pyrococcus furiosus DNA polymerase, (b) Thermus aquaticus DNA polymerase, and (c) a factor that at least partially inhibits the incorporation of dUTP or analogs thereof into a DNA polymer; wherein the factor is: a protein having dUTPase activity and encoded by a nucleic acid having the nucleotide sequence of SEQ ID NO:70 or a nucleic acid that hybridizes to the complete complement of the nucleic acid having the nucleotide sequence of SEQ ID NO:70, wherein the hybridization conditions comprise incubation in 5×SSC and 50% formamide at 42° C., and washing in 0.1×SSC and 0.1% sodium dodecyl sulfate at 60° C.; or a protein having a sequence of amino acids comprising the amino acid sequence of SEQ ID NO:71.