Patent ID: 6924361

Claim:
A preparation of polyclonal antiphosphopeptide-specific antibodies, wherein said antibodies are prepared by the process comprising: (a) generating a polyclonal antiserum that is reactive with a predetermined phosphopeptide, the corresponding non-phosphopeptide and the corresponding phosphoamino acid by immunizing an animal with said phosphopeptide; (b) selectively removing non-phosphopeptide-specific antibodies from said serum by adsorbing said serum with the nonphosphorylated form of said phosphopeptide linked to a solid support; (c) selectively removing phosphoamino acid-specific antibodies from said serum by adsorbing said serum with the phosphoamino acid of said phosphopeptide linked to a solid support; and (d) recovering said antiphosphopeptide-specific antibodies; where steps (b) and (c) can be performed in either order.