Patent ID: 8645083

Claim:
A method for the selection of an antibody from a plurality of antibodies, said method comprising the following steps: (a)—determining at the temperatures used in step (e) the signal of a surface plasmon resonance and adjusting the sensor surface so that a constant Rmax value of antigen-antibody binding is obtained at these temperatures, (b)—measuring BL (RU) and SL (RU) by surface plasmon resonance, (c)—calculating an antigen-complex-stability according to formula (I) antigen-complex-stability=(1 −[BL ( RU )− SL ( RU )/ BL ( RU )]) (I) wherein BL denotes a Binding Late reference point set shortly before the antigen's injection ends, and SL denotes a Stability Late reference point set shortly before the end of the complex dissociation phase, (d)—selecting the antibody with an antigen-complex-stability value greater than 0.95, (e)—measuring BL(RU) and SL(RU) and calculating the antigen-complex-stability of the antibody of (d) at 17° C., at 21° C., at 25° C., at 29° C., at 33° C., and at 37° C., (f)—calculating Transition State thermodynamic properties of the antibody at each temperature of (e) based on equations (II) to (XIII), and (g)—selecting the antibody when a temperature-dependent acceleration of the antigen complex association rate constant k a [1/Ms] is detected and a remaining or decreasing antigen complex dissociation rate constant k d [1/s] is detected, as the temperature increases from 17° C. to 37° C.