Patent ID: 8785180

Claim:
A biochemical reactor, wherein the biochemical reactor is equipped with an in-situ cell examination microscope, wherein the in-situ cell examination microscope comprises a main body of the microscope, an object lens, an observation entrance window, a sampling device and an exterior light source system, wherein the observation entrance window is arranged in front of the main body of the microscope, the sampling device is arranged in front of the observation entrance window, and an object lens and an exterior light source system are both arranged behind the observation entrance window in the main body of the microscope, wherein a reflecting prism is arranged between the exterior light source system and the object lens, a reflector is arranged behind the object lens while in front of the observation entrance window, an annular diaphragm plate is arranged in front of the reflecting prism, and a CCD or an area array image sensor is arranged above one side of the reflecting prism; and wherein the sampling device consists of a sampling piece, a flexible element and a movable device, wherein the sampling piece and a driving axle of the movable device are connected by the flexible element, and the space between the front end face of the sampling piece and the observation entrance window forms a sampling pool; wherein a living cell sensor is further arranged on a body of the biochemical reactor, and wherein the biochemical reactor is capable of measuring physiological metabolic parameters to optimize and scale up a fermentation process, and wherein the flexible element is compressible or stretchable to adjust a distance between the front end face of the sampling piece and the observation entrance window.