Patent ID: 7026142

Claim:
A method for enzymatic conversion of GDP-mannose to GDP-fucose, the method comprising: a) providing a reaction mixture that comprises GDP-mannose, GDP-mannose 4,6-dehydratase, and NADP+; b) incubating the reaction mixture for a sufficient time to convert at least 90% of the GDP-mannose to GDP-4-keto-6-deoxymannose; c) adding to the reaction mixture a polypeptide comprising an amino acid sequence with at least 95% identity to SEQ ID NO:1, wherein the polypeptide has GDP-4-keto-6-deoxymannose 3,5-epimerase and GDP-4-keto-6-galactose reductase activities; and d) incubating the reaction mixture for a sufficient time to convert the GDP-4-keto-6-deoxymannose to GDP-fucose.