Patent ID: 7824695

Claim:
A method of systemically delivering an effective dose of a bioactive moiety to produce a systemic effect in a mammal in need thereof, said method comprising contacting a mucosal membrane of the mammal with a recombinant protein drug that comprises: (1) a cell recognition moiety that binds to an α2 macroglobulin receptor, and (2) the bioactive moiety, which: (a) has a biological activity that mediates the systemic effect of the bioactive moiety and is selected from receptor binding, cytokine activity, enzymatic activity, interleukin activity, neurotransmitter activity, regulation of transcription and affinity for a bioorganic molecule, (b) does not function as an immunogen in producing the needed systemic effect, and (c) does not have ADP ribosylating activity, wherein, the bioactive moiety is translocated across the mucosal membrane of a mammal from the apical surface of the membrane through the basal surface of the membrane to enter a sub-membrane space below the mucosal membrane in an amount which delivers an effective dose of the protein drug to the mammal to produce the systemic effect; and further, wherein, when the protein drug comprises a Pseudomonas exotoxin moiety, the Pseudomonas exotoxin moiety is noncytotoxic.