Patent ID: 8247544

Claim:
A DNAzyme which binds to T-bet mRNA and functionally inactivates it, which comprises: (i) a catalytic domain with the nucleotide sequence GGCTAGCTACAACGA SEQ ID NO: 154 or a modified sequence with comparable biological effect, which cleaves the T-bet mRNA at every purine-pyrimidine binding site to which it is bound; and either (ii) a right substrate binding domain adjoining the 3′-end of the catalytic domain having polynucleotide sequence TGGGTTTCT; and (iii) a left substrate binding domain adjoining the 5′-end of the catalytic domain having polynucleotide sequence GGCAATGAA, both substrate binding domains being respectively complementary to two regions of the T-bet mRNA so that they hybridize with the mRNA, or; (iv) a right substrate binding domain adjoining the 3′-end of the catalytic domain having polynucleotide sequence GAACTGGGT; and (v) a left substrate binding domain adjoining the 5′-end of the catalytic domain having polynucleotide sequence TCACGGCAA, both substrate binding domains being respectively complementary to two regions of the T-bet mRNA so that they hybridize with the mRNA, and which is active in vivo.