Patent ID: 7855053

Claim:
A method for performing PCR to determine the presence or absence of an allele with expanded CGG repeats of the 5′ untranslated region (“UTR”) of the fragile X mental retardation 1 (“FMR1”) gene in a biological sample from a subject, said method comprising amplifying the CGG repeat region of said 5′ UTR region of the gene with a first primer and a second primer, wherein (a) said first primer is complementary to a portion of the 5′ UTR region that is outside of the CGG repeat region; and, (b) said second primer is a chimeric primer comprising 3 to 30 consecutive repeats of CCG or 3 to 30 consecutive repeats of CGG at the 3′ end of the second primer and a random nucleotide sequence at the 5′ end of the second primer.