Patent ID: 7588929

Claim:
A method for intermediate to large scale production in a semi-solid culture medium of stock compositions of bacteriophage having a titer of at least 10 11 pfu/ml and a total yield of at least 10 15 total pfu comprising: (a) growing bacteriophage in a semi-solid culture medium comprising (i) a pre-incubated mixture of at least one bacterial strain and at least one phage type and (ii) a hydrocolloid at a concentration below 0.3% wherein the semi-solid culture medium is supported by a solid phase; (b) incubating the semi-solid culture medium to reach bacterial lysis, thereby obtaining a phage lysate; and (c) extracting a crude bacteriophage extract from the semi-solid culture medium, using an extraction medium, wherein the crude bacteriophage extract is obtained by sequential serial extractions and wherein the titer of the crude bacteriophage extract is at least 10 11 pfu/ml and the bacteriophage yield is at least in the order of magnitude of 10 15 to 10 16 total pfu.