Patent ID: 7276340

Claim:
A method for identifying whether a stain contains blood of a newborn human being, comprising the steps of: a) obtaining a sample stain comprising blood from a human being; b) extracting total ribonucleic acid (tRNA) from the sample stain; c) treating the total RNA with an enzyme; d) initiating a reverse-transcription (RT) reaction by treating total RNA with random decamer primers and a reverse transcriptase enzyme to produce cDNA; e) amplifying the cDNA using HBGv-specific primers consisting of SEQ ID NO: 9 and SEQ ID NO: 7 and HSK primers with corresponding fluorescently labeled probes; f) identifying the age of the donor by determining the cycle threshold (Ct) value of a housekeeping gene (HSK) and the Ct value of the HBGv target gene, then subtracting the Ct value of the target gene, from the Ct value of the HSK gene, wherein a positive value would indicate that the blood originates from a newborn human being.