Patent ID: 8822202

Claim:
A sterile disposable apparatus for preparation of lipoaspirate and isolation of reparative cells from adipose tissue without centrifugation, comprising: a digestion chamber having at least one inlet port and at least one outlet port and a digestion mesh maintained between said at least one inlet and outlet ports of the digestion chamber, said chamber adapted for digestion of adipose tissue across said digestion mesh during enzymatic dissociation of the adipose tissue into a dissociated cell mixture; a lipoaspirate port on the digestion chamber adapted for removal of lipoaspirate or washed lipoaspirate prior to enzymatic dissociation of the adipose tissue; a dispersing filter having an inlet port and an outlet port and in fluid communication with the digestion chamber and configured to filter the dissociated cell mixture into a dispersed cell mixture; and a lipid separating unit including at least one dispersing head having a plurality of pores in fluid communication with the outlet port of the dispersing filter for breaking up cell clusters in the dispersed cell mixture before introduction into an aqueous medium in the lipid separating unit, said lipid separating unit being adapted for fluid phase separation of lipids and adipocytes from reparative cells in the introduced dispersed cell mixture without centrifugation, whereby isolated reparative cells are recoverable from the apparatus.