Patent ID: 8828667

Claim:
A method of detecting diisocyanate-protein conjugates in a sample, comprising: a) contacting a sample with a first isolated monoclonal antibody or antigen binding fragment thereof, wherein the first isolated monoclonal antibody or antigen binding fragment thereof binds to a 2,4-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage; and b) detecting binding of the first isolated monoclonal antibody or antigen binding fragment thereof with the 2,4-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage, and c) contacting the sample with a second monoclonal antibody or antigen binding fragment thereof wherein the second monoclonal antibody or antigen binding fragment thereof binds to 2,6-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage; and d) detecting binding of the second isolated monoclonal antibody or antigen binding fragment thereof with the 2,6-toluene diisocyanate moiety bound to a protein through a urea, urethane, carbamate or thiocarbamate linkage.