Patent ID: 7208179

Claim:
A method of using a supplemented tissue sealant composition to prevent or treat a disease in a patient by releasing into said patient an amount of a supplement effective for prevention or treatment of said disease, said method comprising: (a) preparing a supplemented tissue sealant composition comprising: (i) an effective amount of demineralized bone matrix as a supplement, and (ii) fibrinogen, or a derivative or metabolite thereof, in an amount which is capable of forming a fibrin matrix; and (b) applying said supplemented tissue sealant composition of step (a) to a patient in need thereof at a concentration which releases into said patient an effective amount of said supplement to prevent or treat said disease; wherein said fibrinogen forms a fibrin matrix when in the presence of thrombin and Ca ++ and water, further wherein said supplemented tissue sealant composition adheres to the tissue of said patient, further wherein said supplement is released from said fibrin matrix into the external environment of use for a sustained period, further wherein the amount of said supplement in said composition is greater than the amount of said supplement which is soluble in said fibrin matrix, and further wherein said sustained period is greater than the period obtained according to simple diffusion kinetics, upon dissolution of the fibrin matrix.