Patent ID: 8631865

Claim:
A biotechnological process for oil recovery from an oil well having low permeability rock in the range of 7 to 100 mD, which comprises: a) obtaining a fluid sample and a rock sample from said oil well, characterization of the samples collected, and determining the temperature, pressure and salinity conditions in said oil well; b) formulating a culture media for development of a first culture of extremophile anaerobic indigenous microorganisms, c) culturing microorganisms from said fluid sample (a) in said culture media (b) to isolate extremophile anerobic indigenous microorganisms thereby producing said first culture having characteristics of metabolite production at 60 to 95° C., NaCl concentrations of from 5,000 to 45,000 ppm, while under pressures from 7 to 154.6 Kg/cm 2 (100 to 2,200 psi) in anaerobic conditions, d) providing a second culture containing at least one microorganism selected from the group consisting of Thermoanaerobacter ethanolicus, Thermoanaerobacter uzonensis, Thermoanaerobacter Geothermobacterium sp, Methanobacterium subterraneum, Methanobacterium formicicum, Methanolinea tarda and Methanoculleus sp adapted to a temperature in the range of 60 to 95° C., NaCl concentrations of from 5,000 to 45,000 ppm, and pressures of from 7 to 154.6 Kg/cm 2 (100 to 2,200 psi), e) producing an inoculum for oil recovery comprising a mixture of said first and second cultures, f) injecting said inoculum and water into said oil well and closing said oil well for 5 to 10 days to allow for metabolite production that increases oil recovery.