Patent ID: 7572630

Claim:
An in vitro method for differentiating a primate embryonic stem cell selected from CMK-6 cell strain or HES-3 cell strain into a vascular cell selected from the group consisting of a vascular endothelial cell, a mural cell and a vascular construct, comprising differentiating a primate embryonic stem cell into a vascular endothelial growth factor receptor (VEGFR-2)-positive cell, wherein the differentiated vascular cell lacks at least one of ALP, SSEA-3, SSEA-4, TRA1-60 and TRA1-81, wherein said differentiating comprises the steps of: (I) co-culturing a primate embryonic stem cell with a feeder cell which includes OP9 cell strain, to differentiate into a cell population A comprising said vascular endothelial growth factor receptor (VEGFR-2)-positive and undifferentiated primate stem cell marker-negative cell, and (II) separating substantially said VBGFR-2-positive and undifferentiated primate embryonic stem cell marker-negative cell from the cell population A obtained in the above step (I).