Patent ID: 8603782

Claim:
A method for producing an isolated heat stable polypeptide capable of binding to a target molecule, said heat stable polypeptide comprising at least two LDL receptor A-domains, the method comprising, (a) culturing prokaryote cells that recombinantly express said heat stable polypeptide capable of binding to a target molecule; (b) heating the cells to between 50-100° C. by a continuous flow heating process for a time sufficient to lyse at least 50% of the cells; (c) incubating the resulting intact and lysed cells at a temperature of between −10° C. and 10° C.; and (d) separating the expressed heat stable polypeptides from the intact cells and lysed cells; wherein more than 80% of said separated, expressed, polypeptides are heat stable polypeptides that retain the ability to bind to said target molecule.