Patent ID: 8017369

Claim:
A method of up-regulating the gene expression of bone morphogenetic protein-2 (BMP-2) in targeted tissue, comprising the steps of: generating at least one specific and selective signal having a frequency from 30 kHz to 120 kHz that when applied to a field generating device operatively disposed with respect to said targeted tissue causes the generation of a field having an amplitude of about 2 to 40 mV/cm in the targeted tissue that is specific and selective for the up-regulation of the gene expression of BMP-2 in said targeted tissue as measured by mRNA when said field is applied to the targeted tissue containing said BMP-2; and exposing the targeted tissue to the specific and selective field generated by said field generating device upon application of said at least one specific and selective signal thereto for a predetermined duration of time from approximately ½ hour to 24 hours per 24 hour period at a predetermined duty cycle from approximately 10%-100% so as to selectively up-regulate the gene expression of BMP-2 in said targeted tissue as measured by mRNA.