Patent ID: 8512972

Claim:
A method for identifying a compound that binds to Hypoxia Inducible Factor-1 alpha prolyl hydroxylase (EGLN1), comprising: a) providing a crystal of an EGLN1 catalytic domain polypeptide comprising the amino acid sequence of SEQ ID NO: 3 in complex with [(1-chloro-4-hydroxy-isoquinoline-3-carbonyl)amino]acetic acid, wherein the crystal has unit cell dimensions of a=111±1 Å, b=111±1 Å, and c=40±1 Å, α=90°, β=90°, γ=120° in the hexagonal space group P6 3 ; b) exposing the crystal to a compound in a medium to form a crystal/compound complex; c) irradiating the crystal/compound complex with X-rays to generate a diffraction pattern; d) capturing the pattern to a recording device to generate diffraction data; e) processing the data to solve the structure of the crystal/compound complex; and f) determining the location and binding geometry of the compound within the structure of the complex; thereby identifying a compound that binds to EGLN1.