Patent ID: 8865675

Claim:
A nucleic acid-lipid particle comprising: (a) an siRNA that silences Apolipoprotein B (APOB) expression, wherein the siRNA consists of the following sense and antisense strand sequences: (SEQ ID NO: 4) 5′-AG U G U CA U CACAC UG AA U ACC-3′ (SEQ ID NO: 11) 3′- GUU CACAG U AG U G U GAC U UAU-5′, wherein the bolded and underlined nucleotides are 2′OMe nucleotides; (b) a cationic lipid of Formula I having the following structure: or salts thereof, wherein: R 1 and R 2 are either the same or different and are independently hydrogen (H) or an optionally substituted C 1 -C 6 alkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl, or R 1 and R 2 may join to form an optionally substituted heterocyclic ring; R 3 is either absent or is hydrogen (H) or a C 1 -C 6 alkyl to provide a quaternary amine; R 4 and R 5 are either the same or different and are independently an optionally substituted C 10 -C 24 alkyl, C 10 -C 24 alkenyl, C 10 -C 24 alkynyl, or C 10 -C 24 acyl; X is O, S, N(R 6 ), C(O), C(O)O, OC(O), C(O)N(R 6 ), N(R 6 )C(O), OC(O)N(R 6 ), N(R 6 )C(O)O, C(O)S, C(S)O, S(O), S(O)(O), C(S), or an optionally substituted heterocyclic ring, wherein R 6 is hydrogen (H) or an optionally substituted C 1 -C 10 alkyl, C 2 -C 10 alkenyl, or C 2 -C 10 alkynyl; and Y is either absent or is an optionally substituted C 1 -C 6 alkyl, C 2 -C 6 alkenyl, or C 2 -C 6 alkynyl; and (c) a non-cationic lipid.