Patent ID: 8313907

Claim:
A device for nucleic acid sequence detection, comprising: (a) a vessel for containing a reaction medium including at least one template nucleic acid molecule with components for template polymerization by complimentary addition of one or more nucleotides to the at least one template nucleic acid molecule with a concomitant proton release; (b) at least one polarizable electrode, connected to an amplifying circuit to amplify signals generated in response to polarity changes induced in the at least one polarizable electrode, so as to be sensitive to charge perturbations in the reaction medium whereby the polarity changes in the at least one electrode result in response to charge perturbations from the concomitant proton release as template polymerization of the at least one template nucleic acid molecule; and (c) a detection component, comprising the amplifying circuit, that generates signals in response to the polarity changes induced in the electrode, the signals indicating whether one or more corresponding nucleotides are added to the at least one template nucleic acid to thereby discern at least a portion of the at least one template nucleic acid sequence.