Patent ID: 8379944

Claim:
An imaging system for detecting targets of interest (TOIs) in multispectral imaging data comprising: a memory device storing a plurality of instructions embodying the system for detecting TOIs; a processor for receiving the multispectral imaging data and executing the plurality of instructions to perform a method comprising: determining a list of events collocated across images of the multispectral imaging data; and labeling each event as one of a TOI or non-TOI, wherein the events are emissions of fluorophore labeled structures, and wherein the labeling comprises: generating a filtered imaging data tracking illumination changes and overall background level in the multispectral imaging data; generating compensated imaging data by applying a transform to compensate for illumination in the filtered imaging data and provide a measure of signal-to-noise (S/N) that reflects a brightness perception; and outputting a binary mask, wherein overlapping connected components of the binary mask across frequencies are collocated and output as a list of labels shared across all images of the compensated imaging data, wherein the overlap exceeds a threshold percentage of a largest connected component and none of the connected components in a single channel overlap with more than one connected component in another channel.