Patent ID: 8133714

Claim:
A recombinant coryneform bacterium comprising a SugR regulator, wherein: a) said recombinant coryneform bacterium: i) secretes an organic chemical compound; ii) has the ability to utilize as a carbon source one or more of the compounds selected from the group consisting of: glucose, fructose, sucrose, and acetic acid; and iii) comprises a feedback resistant aspartate kinase; and b) said SugR regulator comprises all of the sequence of SEQ ID NO: 2 except for one or more differences selected from the group consisting of: i) the amino acid at position 37 is selected from the group consisting of: L-alanine, glycine, L-isoleucine and L-proline; ii) the amino acid at position 38 is selected from the group consisting of: L-alanine, glycine, L-isoleucine and L-proline; iii) the amino acid at position 39 is selected from the group consisting of L-alanine, glycine, L-isoleucine and L-proline; iv) the amino acid at position 40 is L-proline; v) the amino acid at position 72 is selected from the group consisting of: L-alanine, glycine, L-glutamic acid and L-aspartic acid; vi) the amino acid at position 101 is selected from the group consisting of: L-arginine, L-lysine, L-phenylalanine, L-methionine, L-glutamine, L-tryptophan, L-tyrosine and L-glutamic acid; vii) the amino acid at position 105 is selected from the group consisting of L-proline, L-phenylalanine, L-isoleucine, L-methionine, L-glutamine, L-tryptophan and L-tyrosine; viii) the amino acid at position 210 is selected from the group consisting of: L-alanine, L-arginine, and L-proline; and ix) the amino acid at position 216 is selected from the group consisting of: L-alanine, L-glutamic acid, L-isoleucine and L-tryptophan.