Patent ID: 8653250

Claim:
A set of reagents consisting of: a first pre-hybridization wash fluid; a second pre-hybridization wash fluid; a hybridization mix; HPV E6, E7 mRNA specific oligonucleotides; a first post-hybridization stringency wash; a second post-hybridization stringency wash; and a permeabilization reagent, wherein said set of reagents when combined with a first and a second antibody, phosphate buffer saline, 2% fetal calf serum and a liquid-based cervical cytology specimen comprising cervical cells having 100 copies or more of HPV E6, E7 mRNA per cell, provides for determining that the cervical cells in the liquid-based cervical cytology specimen having E6, E7 mRNA copies above 100 copies of HPV E6, E7 mRNA per cell are malignant.