Patent ID: 7169395

Claim:
A multi-valent immunogenic composition, which comprises: (a) a mixture of purified fusion (F), attachment (G) and matrix (M) protein of RSV, (b) a non-virulent influenza virus preparation, and (c) an adjuvant, said immunogenic composition being formulated for in vivo administration to the host wherein the individual components (a) and (b) of the composition are formulated such that the immunogenicity of the individual components (a) and (b) is not impaired, wherein said adjuvant is poly-di(carboxylatophenoxy)-phosphazene (PCPP) and is present in an amount which imparts an enhanced immune response to RSV when compared to the mixture (a) formulated with the adjuvant in the absence of the non-virulent virus preparation, and wherein said non-virulent influenza virus preparation is prepared as follows: (i) preparing a sterile suspension of influenza virus propagated in chicken embryos; (ii) inactivating the virus in the suspension with formaldehyde; (iii) concentrating and purifying the virus in a linear sucrose density gradient solution, where said concentrating and purifying is performed using a continuous flow centrifuge; (iv) chemically disrupting the purified inactivated virus using Triton X-100, resulting in a split-antigen; (v) further purifying the split-antigen by chemical means; (vi) suspending the purified split-antigen in a sodium phosphate-buffered isotonic sodium chloride solution; and (vii) adding to the split-antigen solution a 0.05% Gelatin stabilizer and the preservative thimerosol at a 1:10,000 concentration.