Patent ID: 7226770

Claim:
A variant of a fungal parent lipolytic enzyme, which comprises one or more of the following amino acid substitutions: A150 to G; I238 to V and; P250 to N, Q or V; wherein (a) each position corresponds to the position of the amino acid sequence of SEQ ID NO: 1; (b) the variant has lipolytic activity; and (c) the variant has at least 90% homology with SEQ ID NO: 1 determined using the GAP computer program provided in the GCG program package (Program Manual for the Wisconsin Package, Version 8, August 1994, Genetics Computer Group, 575 Science Drive, Madison, Wis., USA 53711) with the following settings for polypeptide sequence comparison: GAP creation penalty of 3.0 and GAP extension penalty of 0.1.