Patent ID: 7816094

Claim:
A method for quantitative analysis of an interaction between HIF-1α and cAMP-responsive element binding protein (CBP) or p300 protein, which comprises the steps of: 1) preparing a fluorescent probe by attaching a fluorescein to peptides derived from the C-terminal transactivation domain of HIF-1α protein having at least 41 but no more than 55 amino acids which are consecutively presented in the full-length amino acid sequence; 2) reacting the fluorescent probe with CBP or p300 proteins wherein the reaction is induced by mixing the fluorescent probe with CBP or p300 protein within ranges from 1:2 to 1:10; and 3) measuring fluorescence polarization (FP) values for the reaction mixture obtained in step 2) and then comparing with that of the fluorescent probe itself to analyze the changes of FP values.