Patent ID: 7972843

Claim:
A composition comprising a set of plasmids, which results in assembly of an infectious segmented negative strand RNA virus upon introduction in a host cell, wherein each plasmid of said set comprises one viral genomic segment of a negative strand RNA virus, and wherein the viral cDNA corresponding to the viral genomic segment is inserted between an RNA polymerase I (pol I) promoter and a regulatory element, which results in expression of vRNA or cRNA with an exact 3′ end, which are in turn inserted between an RNA polymerase II (pol II) promoter and a polyadenylation signal, which results in expression of viral mRNA and a corresponding viral protein, wherein the expression of vRNAs or cRNAs and viral proteins from said set of plasmids results in an infectious segmented negative strand RNA virus and wherein the total number of the plasmids in said composition does not exceed the total number of the gene segments of said negative strand RNA virus.