Patent ID: 8663916

Claim:
A method for preparing an antifungal polypeptide which inhibits growth of a fungus, wherein the method comprises: (i) preparing a nucleic acid molecule by: inserting a nucleic acid sequence encoding a fungal pheromone targeting agent into a selected position of the nucleic acid molecule; and inserting a nucleic acid sequence encoding a channel-forming domain of colicin Ia into the nucleic acid molecule so as to produce a fusion polynucleotide having a nucleic acid sequence encoding the antifungal polypeptide having the fungal pheromone targeting agent linked to the channel-forming domain, wherein the channel-forming domain comprises the amino acid sequence of SEQ ID NO: 8; (ii) transfecting the fusion polynucleotide into a host cell; and (iii) allowing said host cell to produce said antifungal polypeptide.