Patent ID: 7785769

Claim:
A method for assaying biomolecules wherein said assay is selected from the group consisting of a fluorescence assay, a radioactive assay, a magnetic assay and an optical assay, comprising the steps of: (A) functionalizing a support with acrylate groups; (B) forming a polyacrylate hydrogel by reacting a 6-acryloyl-beta-O-methyl monosaccharide with a crosslinker with two or more polymerizable double bonds, and 2-acrylamido hyrdroxyacetic acid, (C) reacting said polyacrylate hydrogel with said acrylate groups of said support to form a polyacrylate hydrogel linked to a support, (D) reacting said biomolecule to be assayed with a second crosslinker and said polyacrylate hydrogel thereby forming a covalent bond between said biomolecule and said second crosslinker and a covalent bond between said second crosslinker and said polyacrylate hydrogel linked to said support, and (E) assaying said covalently bonded biomolecule.