Patent ID: 7943373

Claim:
A reagent for cancer cell detection, comprising a recombinant adenovirus where a replication cassette comprising an hTERT (human telomerase reverse transcriptase) promoter, an adenoviral E1A gene, an IRES sequence and an adenoviral E1B gene in this order is integrated in E1 region of the adenoviral genome and a labeling cassette comprising a gene encoding a GFP (green fluorescence protein) or an EGFP (enhanced green fluorescence protein) and a cytomegalovirus promoter operably linked to the gene encoding the GFP or the EGFP is integrated into the E3 region of the adenoviral genome such that the region encoding adenovirus death protein (ADP) is removed from the adenoviral genome, wherein the rest of the E3A and the E3B region are still present in the adenoviral genome.