Patent ID: 7851174

Claim:
A method for quantitatively determining cholesterol in high-density lipoprotein in a sample, which comprises: reacting the sample in an aqueous medium comprising a nonionic surfactant, polyanion and albumin with i) cholesterol esterase and cholesterol oxidase to form hydrogen peroxide or ii) cholesterol esterase, an oxidized coenzyme and cholesterol dehydrogenase to form a reduced coenzyme, wherein the nonionic surfactant is polyoxyethylene dodecylamine or polyoxyethylene octadecylamine, and the polyanion is dextran sulfate or a salt thereof; measuring formed hydrogen peroxide, or formed reduced coenzyme; correlating a measured value of hydrogen peroxide or a measured value of reduced coenzyme with an amount of cholesterol in high density lipoprotein by using a calibration curve; and determining a concentration of cholesterol in high-density lipoprotein in the sample.