Patent ID: 7173256

Claim:
An element for calibrating fluorescent light emissions comprising: a body having a plurality of regions suitably simultaneously to induce fluorescence so as to fluoresce at a corresponding plurality of fluorescent light emission intensities, certain regions appearing to fluoresce relatively more brightly while other regions appear to fluoresce relatively less brightly; wherein when the body is illuminated and imaged in a same image field and along with a macroscopic specimen also exhibiting fluorescence at multiple areas and intensities then the body serves as an image calibration step wedge, or gauge, where any of intensities, colors, dimension, overall brightness, and color temperature of any and all of the multiple specimen fluorescent areas may be determined to be properly so imaged, meaning that the each and all specimen areas are imaged so as to show other than black, or no image, but less than saturation; wherein, by comparison to the body that is within a same image, illumination of the specimen may be adjusted so that the full range of all its fluorescent emissions, dim to bright, are captured within a single image.