Patent ID: 7045307

Claim:
A high-throughput screening method for an agent that modulates NO synthase activity, the method comprising, (a) substantially simultaneously incubating in a plurality of reaction vessels a reaction mixture comprising NO synthase and labeled arginine as substrate, whereby arginine is converted into citrulline by the NO synthase, wherein each reaction vessel contains a candidate agent; (b) substantially simultaneously separating non-converted arginine from the reaction mixture in each of the plurality of reaction vessels and capturing the non-converted arginine by means of a filter plate membrane; (c) measuring the amount of captured arginine from each of the plurality of reaction vessels corresponding to each individual candidate agent on the filter plate membrane as an indication of the NO synthase activity; and (d) comparing the NO synthase activity in the presence of the candidate agent with NO synthase activity measured in the absence of the candidate agent, wherein a change in the NO synthase by virtue of the presence of candidate agent indicates that the candidate agent modulates NO synthase activity.