Patent ID: 8263759

Claim:
An article of manufacture for detecting the expression of a set of human genes that comprise SFRP1, GATA2, TNNT2, FGFR4, PTPN13, TNFAIP6, CAV1, and PRKAR2b, consisting essentially of: a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a SFRP1 nucleic acid at a temperature of at least 55° C., wherein SFRP1 nucleic acid has the nucleotide sequence of SEQ ID NO:42, a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a GATA 2 nucleic acid at a temperature of at least 55° C., wherein GATA 2 nucleic acid has the nucleotide sequence of SEQ ID NO:44, a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a TNNT2 nucleic acid at a temperature of at least 55° C., wherein TNNT2 nucleic acid has the nucleotide sequence of SEQ ID NO:74, a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a FGFR4 nucleic acid at a temperature of at least 55° C., wherein FGFR4 nucleic acid has the nucleotide sequence of SEQ ID NO:71, a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a PTPN13 nucleic acid at a temperature of at least 55° C., wherein PTPN13 nucleic acid has the nucleotide sequence of SEQ ID NO:16, a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a TNFAIP6 nucleic acid at a temperature of at least 55° C., wherein TNFAIP6 nucleic acid has the nucleotide sequence of SEQ ID NO:18, a probe or a primer or both, or a set of primers or probes or both, that specifically hybridize to a CAV1 nucleic acid at a temperature of at least 55° C., wherein CAV1 nucleic acid has the nucleotide sequence of SEQ ID NO:19, and a probe or a primer, or both, or a set of primers or probes or both, that specifically hybridize to a PRKAR2B nucleic acid at a temperature of at least 55° C., wherein PRKAR2B nucleic acid has the nucleotide sequence of SEQ ID NO:95, and one or more primers or probes or both that specifically hybridize at a temperature of at least 55° C. to one or more different nucleic acid sequences selected from the group consisting of SEQ ID NO:1-2, 4-8, 10-15, 17, 20-33, 35-41, 43, 45, 47, 49-70, 72-73, 75-94, 96, and 100-208.