Patent ID: 7906008

Claim:
A process for quick estimation of BOD of beverage waste water using bio-electrochemical device, wherein the said process comprising the steps of: (a) providing bio-electrochemical device comprising: i) microbes immobilized membrane attached to a commercially available dissolved oxygen probe; ii) multimeter; and iii) a laptop workstation installed with software configured to take “change in current” values in real time and manipulate the values in desired graphical displays; (b) applying an external polarization of −0.62 V to −0.67 V to the microbes immobilized membrane of said bio-electrochemical device; (c) stabilizing the said membrane in 0.025-0.075 M phosphate buffer at pH 6.4-7.2 for 30-45 minutes followed by addition of glucose glutamic acid (GGA) in phosphate buffer; (d) observing the stability of the immobilized microbial membrane through software using stabilized membrane of said device by measuring the change in oxygen concentration in terms of current covering a used range of GGA concentrations; (e) calibrating the change in current for different concentrations of GGA as obtained from step (d) with the conventional BOD values; (f) replacing the GGA solution with fresh phosphate buffer and stabilizing the assembly; (g) adding the beverage waste water in 0.025-0.075 M phosphate buffer at pH 6.4-7.2 to maintain the percentage as determined by software; (h) observing the change in oxygen concentration in terms of current with the help of software; (i) calculating the BOD values using software with the help of calibration curve by putting the change in current as observed in step (h).