Patent ID: 7005129

Claim:
A method for producing antisera containing antibodies specific to a mutant endotoxin from an htrB mutant non-typeable Haemophilus influenzae , the method comprising (a) immunizing an individual with a formulation comprising: (1) an htrB mutant non-typeable Haemophilus influenzae having a mutant endotoxin, (2) the mutant endotoxin, or (3) the mutant endotoxin conjugated to a carrier protein, wherein the mutant endotoxin contains a decreased phosphoethanolamine content and an increased hexose content in the mutant endotoxin's inner core, and a pentaaccylated or tetraacylated lipid A lacking one or two secondary acyl chains compared to the corresponding wild-type non-typeable Haemophilus influenzae hexaacylated endotoxin, and wherein the mutant endotoxin has substantially reduced toxicity as compared to the wild-type non-typeable Haemophilus influenzae hexaacylated endotoxim; and (b) collecting the antisera containing the antibodies from the immunuzed individual.