Patent ID: 7732153

Claim:
A method for detecting an analyte in a sample in an assay procedure comprising: a) providing a sample containing or suspected to contain the analyte and a solid support having immobilized thereon 1) a chemiluminescent compound comprising a chemiluminescent moiety and a linking moiety that links the chemiluminescent moiety to the solid support, wherein the chemiluminescent moiety is of formula I wherein R 1 , R 2 and R 3 are organic groups containing from 1 to 50 non-hydrogen atoms, and each of R 4 -R 11 is hydrogen or a noninterfering substituent, wherein at least one of the groups R 1 -R 11 comprises the linking moiety, and 2) a first specific binding partner for the analyte; b) contacting the sample and the solid support to permit analyte to bind to the immobilized specific binding partner for the analyte; c) providing an excess of an activator compound conjugate comprising an activator compound conjugated to either a second specific binding partner for the analyte or to the analyte, wherein the activator compound is a peroxidase enzyme; d) permitting the activator compound conjugate to undergo a specific binding pair reaction thereby bringing a first portion of the activator compound conjugate into proximity to the immobilized chemiluminescent compound, wherein a second portion of the activator compound conjugate does not undergo a specific binding pair reaction and is not brought into proximity to the immobilized chemiluminescent compound, and wherein the portion of the activator compound conjugate in proximity to the immobilized chemiluminescent compound activates a portion of the immobilized chemiluminescent compound; e) without removing the second portion of the activator compound conjugate, providing a trigger solution to produce chemiluminescence from the activated portion of the chemiluminescent compound wherein the trigger solution is an aqueous solution comprising a peroxide compound; f) detecting the chemiluminescence produced; and g) relating the chemiluminescence produced to the presence, location, or amount of the analyte in the sample.