Patent ID: 7083779

Claim:
A process for the preparation of a formulation comprising 2 to 5.5% w/v ( Azadirachta indica from bark and leaves); 0.5 to 2.5% w/v ( Citrullus colocynthis ); 0.1 to 0.4% w/v ( Cucumis sativus ): 82–97% w/v of carrier or additive, wherein the said process comprising the steps of: a. extracting said bark and leaves using a commercial blender at a temperature of 10–25° C., preferably at 20° C. and in an aqueous solution of polar solvents; b. excluding tannins from the extract of step a by liquid phase extraction using a non-polar organic solvent and a polar solvent; c. concentrating and partitioning the above extract obtained from step (b) by liquid phase extraction using a polar and a non-polar solvent and obtaining a polar fraction comprising rich in nimbolides as component A; d. extracting plant parts of Citrullus colocynthis , preferably underground parts, using a homogenizer at 7000 to 8000 revolution per minute in an aqueous polar solvent; e. purifying the active constituents from above extract in step (d) by concentrating and partitioning through liquid phase extraction using a polar and a non-polar solvent to obtain polar phase as component B; f. extracting an anti-oxidant fraction from Cucumis sativus by cold extraction using aqueous polar solvents at temperature ranging from 10–25° C. using a tissue homogenizer, to obtain the polar fraction as component C; g. mixing component A from Azadirachta indica , component B from C. colocynthis , component C from C. sativus , a carrier in a ratio ranging from 2 to 5.5% w/v, 0.5 to 2.5% w/v, 0.1 to 0.4% w/v, and 82–97% w/v respectively to obtaining a herbal synergistic dental care formulation.