Patent ID: 8530194

Claim:
A DNA polymerase inhibitor suitable for binding to a Pol domain and a 5′-exo domain of a DNA polymerase, said inhibitor comprising: at least one oligonucleotide, said oligonucleotide including: a first section of DNA, and a second section of DNA, wherein: said first section comprises a primer-like oligonucleotide of about 8 to about 50 nucleotides, and further comprises an oligonucleotide complementary to said primer-like oligonucleotide, and wherein said first section of DNA forms a double stranded DNA structure capable of binding to the Pol domain of a DNA polymerase, said second section comprises a 5′-exo oligonucleotide of about 3 to about 50 nucleotides and an oligonucleotide complementary to said 5′-exo oligonucleotide, and wherein said second section of DNA forms a double stranded structure capable of binding to the 5′-exo domain of said polymerase, and said second section includes at least two nucleotides joined by a backbone linkage wherein at least one of said backbone linkages is resistant to 5′-exonuclease activity.