Patent ID: 8048361

Claim:
A method for forming a porous bio-mimicking scaffold, consisting essentially of the following order of process steps: providing at least two types of solutions wherein at least one of said solutions is a bio-mimicking scaffold solution comprising a bio-mimicking scaffold and at least one of said solutions is a pore forming solution comprising a pore forming material having at least one corresponding specific solvent; performing a filling process to fill said solutions into different needles of a blending injection device; applying a first electric property to said blending injection device and applying a second electric property to a collecting device wherein said first electric property is opposite to said second electric property, a potential difference exists between said blending injection device and said collecting device, the distance between said blending injection device and said collecting device is defined as an electric field distance, and said electric field distance divided by said potential difference is electric field intensity; performing an electrospinning process to have said solutions emitted from said needles of said blending injection device toward said collecting device so as to form a composite material on said collecting device wherein said composite material comprises both nanometer and micrometer scales of fibers, particles or combination of fibers and particles; performing a crosslinking process using a crosslinking reagent to have said bio-mimicking scaffold material undergo crosslinking, wherein said crosslinking reagent is selected from the group consisting of the following or any combination thereof: glutaraldehyde and formaldehyde; and, performing a removing process by using said solvent to dissolve said pore forming material to remove said pore forming material to thereby form a porous bio-mimicking scaffold; wherein said pore forming material is selected from the group consisting of the following or any combination thereof: chitosan, poly lactic acid, poly caprolactone-co-lactic acid, collagen, polyamide, and gelatin; two type of pores, one having a nanometer-scaled pore diameter and the other having a micrometer-scaled pore diameter, are formed; and said solvent in the removing process is selected from the group consisting of the following or any combination thereof: acetone and 1,4-dioxane.