Patent ID: 8304233

Claim:
A site-specific integrating expression cassette (INTEC) vector, wherein the INTEC vector consists of: (a) a polynucleotide of interest operably linked to a promoter, (b) a single recombination site selected from an attP site, an attB site, a pseudo attP site, and a pseudo attB site that is a substrate for recombination by a unidirectional site-specific recombinase selected from a φC31 phage recombinase, a TP901-1 phage recombinase, or an R4 phage recombinase, wherein said single recombination site is a substrate for recombination with a recombination site in the genome of a target cell by said unidirectional site-specific recombinase, and (c) a hybrid recombination site selected from an attL site and an attR site, wherein the hybrid recombination site is not a substrate for said unidirectional site-specific recombinase.