Patent ID: 6887681

Claim:
A method for assaying a surface suspected of contamination by the presence of cellular material on the surface, the method comprising the steps of: a.) contacting the surface with a sampling swab wetted with a wetting solution so as to obtain a sample of at least a portion of the cellular material, if any, present on the surface wherein the swab is on a wand having a sealing means on the wand; b.) transferring the sampling swab to an analysis structure; c.) pressing the sampling wand into the analysis structure to a first operational position so that the sealing means has a sealing fit, and a piercing member in the analysis structure pierces the sampling swab and a frangible seal, and enters a reservoir within the wand to release a reagent solution, thereby compressing the swab and washing the sample, if any, through and from the swab so as to provide a sample solution; d.) advancing the wand to a second operational position as the sealing means is providing the sealing fit between the wand and the analysis structure to move the sample solution into a reaction well; e.) compressing the swab against a base to remove any remaining sample solution, so that the sample solution is brought into contact with a reactant mixture in the reaction well to produce bioluminescent radiation; f.) placing the reaction well in the analysis structure in close proximity to a photon detector of a luminometer in absence of light; and g.) monitoring an output signal of the luminometer for an indication of emission of the bioluminescent radiation from the reaction well of the analysis structure indicative of the presence of bacterial cellular material in the sample, wherein the analysis member is removed from the luminometer for disposal.