Patent ID: 7083959

Claim:
A method of making a non-immunogenic construct comprising at least two copies of an epitope of a T-dependent antigen bound to a pharmaceutically acceptable non-immunogenic carrier, which copies bind to a B cell membrane immunoglobulin receptor specific for the epitope but fail to form an immunon, comprising (a) providing a non-immunogenic soluble carrier that has been subjected to a preparative sizing technique to remove substantially most high molecular weight soluble carrier molecules, wherein the carrier is not poly (D-Glu/D-Lys), and an epitope molecule of a T-dependent antigen; (b) coupling two or more of the epitope molecules to the non-immunogenic soluble carrier that has been subjected to the preparative sizing technique of step (a) to yield a conjugate preparation; and (c) subjecting the conjugate preparation to size fractionation to yield a non-immunogenic epitope coupled construct, thereby yielding a non-immunogenic construct which is free of high molecular weight immunostimulatory molecules.