Patent ID: 8691577

Claim:
A method of regenerating Jatropha via somatic embryogenesis comprising the steps: (a) culturing a Jatropha explant on a first medium comprising MS basal medium and 2,4-dichlorophenoxy acetic acid (2,4-D) in the dark to induce embryogenic callus formation, wherein the Jatropha explant is a dissected ovule from an unopened flower bud; (b) culturing the embryogenic callus on the first medium in a light/dark photoperiod to induce somatic embryo development and maturation; and (c) culturing mature somatic embryos on a second medium comprising MS basal medium, indole-3-butyric acid (IBA) and gibberellic acid (GA 3 ) in a light/dark photoperiod to germinate plantlets.