Patent ID: 7939643

Claim:
A process for purifying wild-type von Willebrand factor (VWF) from a plasma fraction comprising steps of: (i) providing a sample of plasma fraction containing wild-type VWF and one or more contaminating proteins comprising fibronectin and/or fibrinogen; (ii) preparing the sample of plasma fraction in a first running buffer at pH of 7.0-7.5 that consists essentially of 10-50 mM sodium and/or potassium phosphate, (iii) performing flow chromatography by loading the sample of plasma fraction to a hydroxylapatite matrix under conditions that 90% or more of fibronectin and/or fibrinogen bind to the hydroxylapatite matrix, while at the same time, less than 10% of the wild-type VWF bind to the hydroxylapatite matrix thereby providing a flow through fraction(s) containing purified wild-type VWF, wherein the flow chromatography is performed with the first running buffer; and (iv) purifying wild-type VWF by collecting unbound wild-type VWF from the flow through fraction(s).