Patent ID: 7569344

Claim:
A method for detecting human papilloma virus (HPV) DNA in a cell sample which indicates the patient providing the cell sample is at risk for cancer, comprising: (a) adding a reagent comprising a plurality of genomic HPV DNA probe sets to the cell sample under suitable hybridization conditions, wherein: (i) a first genomic HPV DNA probe set comprises a plurality of labeled nucleic acid fragments prepared by labeling essentially the full-length genomic sequence of HPV type 16, and which constitute approximately 8.3% of the total HPV DNA in the reagent, (ii) a second genomic HPV DNA probe set comprises a plurality of labeled nucleic acid fragments prepared by labeling essentially the full-length genomic sequence of HPV type 18, and which constitute approximately 20.8% of the total HPV DNA in the reagent, (iii) a third genomic HPV DNA probe set comprises a plurality of labeled nucleic acid fragments prepared by labeling essentially the full-length genomic sequence of HPV type 31, and which constitute approximately 8.3% of the total HPV DNA in the reagent, (iv) a fourth genomic HPV DNA probe set comprises a plurality of labeled nucleic acid fragments prepared by labeling essentially the full-length genomic sequence of HPV type 33, and which constitute approximately 20.8% of the total HPV DNA in the reagent, (v) a fifth genomic HPV DNA probe set comprises a plurality of labeled nucleic acid fragments prepared by labeling essentially the full-length genomic sequence of HPV type 35, and which constitute approximately 20.8% of the total HPV DNA in the reagent, and (vi) a sixth genomic HPV DNA probe set comprises a plurality of labeled nucleic acid fragments prepared by labeling essentially the full-length genomic sequence of HPV type 51, and which constitute approximately 20.8% of the total HPV DNA in the reagent; wherein the labeled nucleic acid fragments of the genomic HPV DNA probe sets detectably hybridize to the genomic sequence of HPV types 39, 45, 52, 56, 58, 59, 68 and 70 in addition to detectably hybridizing to the genomic sequence of HPV types 16, 18, 31, 33, 35, and 51; and wherein the labeled nucleic acid fragments of the genomic HPV DNA probe sets do not detectably hybridize to the genomic sequence of HPV types 42, 43, or 44; and (b) determining whether the labeled nucleic acid fragments of the genomic HPV DNA probe sets detectably hybridize to HPV DNA in the cell sample.