Patent ID: 8546127

Claim:
An apparatus for collection of a target material from a liquid sample comprising target species that contain said target material therein, said apparatus comprising a fluid flow conduit having an upstream inlet and downstream outlet; a filter medium between said fluid flow inlet and said downstream outlet; said filter medium having a pore size adapted to retain said target species thereon and pass, as filtrate, lysate containing said target material to a downstream portion of said fluid flow conduit located downstream from said filter medium and upstream from said downstream outlet; a lysing agent conduit adapted to deliver lysing agent to said filter medium to lyse said target species thereby releasing said target material, said lysing agent conduit comprising a recirculation conduit means for receiving flow of said filtrate therein and recycling said lysing agent to said filter medium, said recirculation conduit means comprising collection means for collecting said target material therein; and a check valve located within said fluid flow conduit and positioned adjacent to one of said upstream inlet or downstream outlet, wherein the check valve is configured to allow fluid flow in only one direction therethrough.