Patent ID: 8422632

Claim:
A method of analyzing one or more crystals in an integrated fluidic circuit having a microchamber disposed in a flow layer and a control layer adjacent the flow layer, the method comprising: dispensing a sample into a sample port in fluid communication with the microchamber; transferring the sample through the flow layer to the microchamber; dispensing a reagent into a reagent port in fluid communication with the microchamber; mixing the sample and the reagent in the microchamber; forming the one or more crystals in the microchamber; irradiating the one or more crystals in the microchamber with an x-ray analysis beam, wherein the x-ray analysis beam passes through: the one or more crystals in the microchamber present in the flow layer; through a membrane disposed between the microchamber and the control layer; through a first portion of the control layer defined by a first material; and through a second portion of the control layer defined by a second material different from the first material; and detecting the x-ray analysis beam.