Patent ID: 8617808

Claim:
A method for detecting the production of more than one analyte by cells in a sample, which method comprises: (a) introducing a sample of cells into a well, wherein (i) a first binding protein which specifically binds to a first analyte; (ii) a second binding protein which specifically binds to a second analyte; and (iii) optionally one or more further binding protein which specifically binds to one or more further analyte; are each immobilized at one or more discrete locations on a solid surface within the well, wherein said discrete locations are spots; (b) culturing said sample of cells under conditions suitable for: (i) release of said first analyte, said second analyte and optionally said one or more further analyte by said cells; and (ii) binding of said first analyte to said first binding protein, binding of said second analyte to said second binding protein and optionally binding of said one or more further analyte to said one or more further binding proteins; and (c) determining whether or not said first analyte is bound to said first binding protein, whether or not said second analyte is bound to said second binding protein, and whether or not said one or more further analyte is bound to said one or more further binding proteins thereby detecting production by a sample of cells of more than one analyte.