Patent ID: 6861214

Claim:
A method of detecting a target biopolymer contained in a sample, comprising the steps of: (a) providing a substrate with a surface; (b) modifying the surface of the substrate by introducing a functionality selected from a group consisting of amino group, carboxyl group, and thiol group on the surface to obtain a modified surface; (c) providing a probe biopolymer that can form a complex with the target biopolymer; (d) contacting either the probe or target biopolymer with the modified surface of the substrate and drying the substrate whereby either the probe or target biopolymer directly adsorbs and immobilizes on the modified surface without additional fixing steps and without chemical crosslinking to form a probe assay article or a target assay article, respectively; (e) contacting the probe assay article with the target biopolymer, or contacting the target assay article with the probe biopolymer under a condition that allows the formation of a complex comprising the probe and the target biopolymers; and (f) detecting and determining the presence of the complex as a measurement for the presence or the amount of the target biopolymer contained in the sample.