Patent ID: 8715996

Claim:
An isolated Thermoanaerobacter brockii β-glucosidase polypeptide variant comprising an amino acid sequence that has at least about 95% sequence identity to wild Thermoanaerobacter brockii β-glucosidase comprising the amino acid sequence of SEQ ID NO: 2 and having at least one substitution of an amino acid residue at a position selected from the group consisting of F11, N27, S34, Y47, K48, E64, I81, A82, P84, K103, R111, Y129, K131, G134, K142, K150, E153, A158, I159, H202, A205, K215, I221, T222, Y229, A231, L239, A241, D254, I256, F257, E285, T286, I291, I303, D307, W328, I330, S334, M351, Y352, L383, F389, K397, H412, T427, K429, V442, D445, and D446, wherein said amino acid residue is determined by alignment with SEQ ID NO: 2.