Patent ID: 8460863

Claim:
A method for the preparation of a dry stick test device for the determination of an analyte in a milk sample by means of a chemical assay, said method comprising the steps of: (i) providing at least one reagent pad by impregnating a first porous material with an aqueous solution comprising a reagent capable of reacting with said analyte, a derivative of said analyte or an indicator compound for said analyte to provide a detectable signal when in a moistened state, (ii) thereafter drying said reagent pad, (iii) providing a development pad by impregnating a second porous material with an aqueous solution comprising at least one controlling compound which, when in a moistened state, is capable of providing a condition required for said reagent to react with said analyte to provide said detectable signal, (iv) thereafter drying said impregnated second porous material, and (v) immobilizing said first porous material with said second porous material, on a solid support, to obtain said dry stick test device, said dry stick test device comprising: said reagent pad comprising said reagent capable of reacting with said analyte, said derivative of said analyte, or said indicator compound for said analyte to provide said detectable signal when in a moistened state, and said development pad which is located in contact with said reagent pad, said development pad comprising said controlling compound capable of providing said condition required for said reagent to react with said analyte to provide said detectable signal, wherein said development pad is located downstream from said reagent pad, wherein said reagent pad and said development pad are arranged to avoid precipitation of a sample component on a top-face of said dry stick test device and wherein said milk sample is applied on said top-face of said dry stick test device and said detectable signal is obtained from said top-face of said dry stick test device, and wherein said controlling compound is an acidic compound and wherein said top-face of said dry stick test device relates to a surface where said milk sample initially contacts said reagent pad.