Patent ID: 7294499

Claim:
A variant TY145 like subtilase comprising three calcium ion binding sites prior to alteration, an alteration at a position corresponding to position 171 in the amino acid sequence of SEQ ID NO:1, and at least one other alteration at one or more positions corresponding to positions in the amino acid sequence of SEQ ID NO:1 selected from the group consisting of: 1, 2, 3, 5, 6, 7, 16, 17, 18, 20, 21, 22, 23, 31, 35, 36, 38, 39, 40, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 59, 60, 61, 62, 63, 64, 66, 67, 68, 69, 70, 71, 72, 73, 79, 80, 81, 84, 85, 87, 88, 89, 90, 91, 92, 106, 107, 108, 109, 110, 112, 113, 114, 115, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 130, 131, 139, 141, 142, 145, 146, 147, 149, 150, 151, 153, 154, 155, 156, 157, 158, 159, 160, 161, 164, 165, 166, 167, 168, 169, 170, 172, 174, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 193, 198, 199, 200, 201, 202, 204, 207, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 239, 240, 241, 242, 243, 247, 249, 250, 252, 254, 265, 268, 269, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 304, 305, 306 and 307; wherein the variant TY145 like subtilase has protease activity and has an amino acid sequence which is at least 90% homologous to SEQ ID NO:1.