Patent ID: 6846971

Claim:
A method of transforming Acacia mangium with a gene of interest comprising the steps of: a) activating Agrobacterium tumefaciens comprising said gene of interest by culturing said Agrobacterium in induction medium comprising acetosyringone; b) preculturing an explant of Acacia mangium selected from the group comprising a stem, a leaflet, a petiole and a bud in medium comprising supplemented basic MS medium, wherein said explant is soaked in 0.5 M mannitol prior to the co-cultivation; c) co-cultivating said activated Agrobacterium tumefaciens and said precultured explant in medium comprising supplemented basic MS medium to produce infected explants; d) culturing said infected explants in medium comprising supplemented basic MS to induce callus and adventitious buds; and e) culturing said callus or adventitious buds on a selective medium comprising supplemented basic MS medium; wherein in said supplemented basic MS medium comprises a) thidiazuron, b) indole-3-acetic acid, c) casein enzymatic hydrolysate, d) L-ascorbic acid, e) L-glutamirie, f) L-asparacine, g) L-proline, h) sucrose and l) phytagel or agar.