Patent ID: 8623599

Claim:
A method for methylation analysis, comprising a) treating genomic DNA with one or more reagents to convert unmethylated cytosine bases to uracil sulfonate or to another base having a different binding behavior than cytosine, while methylated cytosine remains unchanged; b) amplifying the treated DNA by means of i) at least one oligonucleotide comprising or consisting essentially of a sequence as defined by SEQ ID NO: 5 or sequence variants derived from said sequence by 5′-terminal and/or 3′-terminal deletion of 1, 2, 3, 4 or 5 nucleotides; and ii) at least one oligonucleotide comprising or consisting essentially of a sequence as defined by SEQ ID NO: 63 or sequence variants derived from said sequence by 5′-terminal and/or 3′-terminal deletion of 1, 2, 3, or nucleotides; wherein said oligonucleotides are suitable for use as primers; and optionally, a polymerase; and c) deducing the presence or absence of methylation of the CpG dinucleotides amplified in step b) from the results of step b).