Patent ID: 7901892

Claim:
A method of screening an oligonucleotide array for insufficient deprotection or insufficient elongation of oligonucleotides therein, said method comprising the steps of: (a) providing an oligonucleotide array comprising a substrate having a plurality of different oligonucleotides immobilized thereon, with said different oligonucleotides immobilized in different separate and discrete locations on said substrate; (b) providing an antibody that specifically binds to a synthetic oligonucleotide having an organic protecting group covalently bound thereto, which antibody does not bind to said synthetic oligonucleotide when said organic protecting group is not covalently bound thereto; and then (c) contacting said antibody to said oligonucleotide array to thereby detect the presence or absence of binding of said antibody selected and discrete locations on said array, the presence of binding to separate and discrete locations in said array indicating insufficient deprotection or insufficient elongation of oligonucleotides therein.