Patent ID: 7824879

Claim:
A cassette for determining an amount of low density lipoprotein associated cholesterol (LDL C) in a body fluid sample containing very low density lipoprotein (VLDL), chylomicrons, low density lipoprotein (LDL), high density lipoprotein (HDL), and triglycerides (TG) comprising: (a) a body having a sample well for receiving a sample fluid; (b) first and second detection zones mounted on said body for producing a detectable product related to the amount of HDL and an adjusted total cholesterol composed of HDL plus LDL without VLDL and chylomicrons, respectively, in said sample fluid, (c) fluid flow pathways for transferring sample fluid from the sample well to said detection zones, (d) at least a first reagent in one or more of said fluid flow pathways and optionally, in said sample well, for selectively removing VLDL and chylomicrons without removing LDL in sample fluid transferred from the sample well to the first and second detection zones, and at least a second reagent for selectively removing LDL in sample fluid transferred from the sample well to the first detection zone.