Patent ID: 7056679

Claim:
A method of selecting target and target binder pairs comprising: (a) preparing a phage comprising a library of biological targets comprising polypeptides encoded by a cDNA library and a distinguishable first selection marker; (b) obtaining a peptide target binder library selected from the group consisting of a random peptide phage target binder library and a cDNA library encoding a polypeptide, and a second selection marker, and the first selection marker is different from the second selection market; (c) panning, where the target phage and the target binder phage are mixed in solution and targets and target binders are allowed to mate or form complexed pairs; (d) transforming host cells with a mixture of the target phage and target binder phage libraries, wherein the mated or complexed pairs infect the host cells; and selecting for host cells infected by phage that formed complexes of target and target binders based on the selection markers.