Patent ID: 7727715

Claim:
A method of selecting a tobacco that produces a reduced expression of Sequestosome 1 in human cells comprising: (a) providing a first tobacco; (b) obtaining smoke from said first tobacco; (c) contacting a first isolated population of human cells with said smoke from said first tobacco; (d) measuring the level of expression of Sequestosome 1 in said first isolated population of human cells in response to said contact, with said smoke from said first tobacco; (e) providing a second tobacco; (f) obtaining smoke from said second tobacco; (g) contacting a second isolated population of human cells with said smoke from said second tobacco; (h) measuring the level of expression of Sequestosome 1 in said second isolated population of human cells in response to said contact with said smoke from said second tobacco; (i) comparing the level of expression of Sequestosome 1 in said first isolated population of human cells measured in step (d) with the level of expression of Sequestosome 1 in said second isolated population of human cells measured in step (h); and (j) identifying the tobacco that produced a reduced expression of Sequestosome 1 in an isolated population of human cells in response to contact with smoke from said tobacco.