Patent ID: 8202978

Claim:
A composition for amplifying in a transcription associated amplification reaction and detecting in real time a Streptococcus pyogenes target nucleic acid sequence, said composition comprising: a first primer, the base sequence of which consists of a 3′ terminal target-complementary sequence and, optionally, a first primer upstream promoter sequence that is non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said 3′ terminal target-complementary sequence of said first primer being selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 and SEQ ID NO:13, allowing for the presence of RNA equivalents and nucleotide analogs; a second primer, the base sequence of which consists of a 3′ terminal target-complementary sequence and, optionally, a second primer upstream promoter sequence that is non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said 3′ terminal target-complementary sequence of said second primer being selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7, allowing for the presence of RNA equivalents and nucleotide analogs; and a molecular beacon probe, the base sequence of which consists of a target-complementary sequence and a pair of mutually complementary base sequences that are non-complementary to a nucleic acid containing said target nucleic acid sequence or its complement, said target-complementary sequence of said probe being selected from the group consisting of SEQ ID NO:23, SEQ ID NO:24 and SEQ ID NO:25, allowing for the presence of DNA equivalents and nucleotide analogs.