Patent ID: 6890741

Claim:
A method of detecting at least first and second target molecules in a sample comprising: a) contacting said first and second target molecule with a composition comprising: i) an amplification enzyme; and ii) first and second target probes, said first and second target probes comprising: (a) a first and a second bioactive agent, respectively, wherein said first and second bioactive agents specifically bind to said first and second target molecules, respectively; (b) a first and a second adapter sequence, respectively, wherein said first adapter sequence identifies said first target molecule and said second adapter sequence identifies said second target molecule; and (c) at least a first and a second upstream universal priming sequences; b) amplifying said first and second adapter sequences using said first and second universal priming sequences, wherein no ligation is performed, to form first and second amplicons, respectively; c) detecting said first and second amplicons, respectively, to indicate the presence of said first and second target molecules in said sample.