Patent ID: 8354226

Claim:
A method of detecting human parvovirus B19 nucleic acid in a biological sample, comprising the steps of: amplifying in vitro a portion of a parvovirus B19 nucleic acid contained in a biological sample by using at least one nucleic acid polymerase activity and at least one first amplification oligomer and one second amplification oligomer, selected from the group consisting of a first amplification oligomer consisting of SEQ ID NO:5 or SEQ ID NO:6 optionally including a promoter sequence joined to a 5′ terminus of SEQ ID NO:6, and a second amplification oligomer consisting of SEQ ID NO:13; and detecting an amplified product of the parvovirus B19 nucleic acid by using a labeled detection probe that hybridizes specifically with the amplified product, thereby indicating presence of parvovirus B19 nucleic acid in the biological sample.