Patent ID: 8361933

Claim:
A composition comprising: a host cell, wherein the host cell is a bacterial cell; and a fusion protein disposed in an outer membrane of the host cell, the fusion protein comprising (a) a circularly permuted bacterial outer membrane protein, comprising a bacterial OmpX protein or a bacterial OmpA protein, wherein (i) the native N-terminus and the native C-terminus of the bacterial OmpX protein or the bacterial OmpA protein are joined by a linker, wherein the linker is a short flexible peptide, and (ii) a non-native N-terminus and a non-native C-terminus are derived from an extracellular loop of the bacterial OmpX protein or the bacterial OmpA protein, wherein at least one of the non-native N-terminus and the non-native C-terminus is positioned extracellularly, and (b) a passenger polypeptide linked or fused directly to the non-native N-terminus or the non-native C-terminus of the circularly permuted bacterial outer membrane protein, wherein the passenger polypeptide is displayed on the outer membrane of the host cell.