Patent ID: 8486642

Claim:
A method of synthesizing a compound of Formula I: wherein: R 1 is a label wherein said label is selected from the group consisting of biotin, fluorophores, antigens, porphyrins, and radioactive isotopes; L is present or absent and when present is a linking group; and x represents an integer from 1 to 10; said method comprising: reacting 3-ethoxy-2-cyclohexen-1-one with a halo alkyl t-butyldimethylsilyl (TBDMS) ether in hexamethylphosphoramide to form a solution of 6-(alkyl TBDMS ether)-3-ethoxy-2-cyclohexen-1-one, adding tetra butyl ammonium fluoride (TBAF) to said solution of 6-(alkyl TBDMS ether)-3-ethoxy-2-cyclohexen-1-one to form a solution of alcohol, adding a synthetic precursor of said label to the solution of alcohol to form a solution of labeled 6-alkyl-3-ethoxy-2-cyclohexen-1-one, and adding HCl to said solution of labeled 6-alkyl-3-ethoxy-2-cyclohexen-1-one to make said compound of Formula I.