Patent ID: 8551994

Claim:
A method for purifying fibrinogen or a protein that is a fibrinogen analogue, wherein said method comprises contacting a sample containing fibrinogen or a protein that is a fibrinogen analogue with an affinity adsorbent of formula II: wherein one X is N and the other is N, C—Cl or C—CN; Y is O, S or NR 2 ; Z is O, S or NR 3 ; R 2 and R 3 are each H, alkyl, hydroxyalkyl, benzyl or β-phenylethyl; n is 0 to 6; A is a support matrix selected from agarose, silica, cellulose, dextran, starch, alginate, carrageenan, synthetic polymers, glass and metal oxides, wherein A is optionally linked to the triazine ring by a spacer; R 7 is a group bearing a positive charge at neutral pH; W is an optional linker; and either V is an aromatic group, R 8 is H, OH, alkyl, alkoxy, amino, acyloxy, acylamino, CO 2 H, sulphonic acid, carbamoyl, sulphamoyl, alkylsulphonyl or halogen and R 9 is a morpholino group, or VR 8 R 9 is morpholine, wherein the fibrinogen, or the protein that is a fibrinogen analogue, binds to the affinity adsorbent; and wherein said method further comprises recovering the fibrinogen or the protein that is a fibrinogen analogue from the affinity adsorbent, thereby obtaining purified fibrinogen or purified fibrinogen analogue with a purity of greater than 85%.