Patent ID: 8497098

Claim:
Process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of ornithine decarboxylase (ODC) activity as compared to the native level of ornithine decarboxylase activity, wherein there is increased N-acetylglutamate formation in said microorganism as compared to the native level of N-acetylglutamate formation; the process comprising: (a) culturing said microorganism to excrete 1,4-butanediamine into a fermentation broth, whereby said increased ODC activity is achieved by overexpression of an ornithine decarboxylase encoding gene speF or speC, each belonging to E.C.4.1.1.17, and the increased activity of N-acetylglutamate formation is achieved by overexpression of at least one of an N-acetyleglutamate synthase encoding argA (belonging to E.C.2.3.1.1) and an N 2 -acetyl-L-ornithine:L-gluamate N-acetyl transferase encoding gene argJ (belonging to E.C.2.3.1.35), and (b) recovering said 1,4-butanediamine from the fermentation broth.