Patent ID: 7585628

Claim:
A method of detecting or analyzing nucleic acid by simultaneously amplifying not less than two types of target nucleic acid samples having base sequences different in at least one base by using not less than two types of primers specific to sites of base sequences different in at least one base or not less than two types of primers specific to mutually different base sequences and analyzing a proportion of an amplified amount of nucleic acid of a target gene site in a target nucleic acid; the method comprising steps of: amplifying nucleic acid by use of an amplification reaction solution containing the target nucleic acid; estimating the number of copies of the target nucleic acid contained in the amplification reaction solution and the number of copies of initial target nucleic acid based on the amplified amount of nucleic acid after amplification or during amplification; determining the number of copies of the target nucleic acid required in the amplification reaction solution by use of the number of copies of the initial target nucleic acid on the assumption that the probability that the number of the target nucleic acid copies in an aliquot is more than the necessary number of the target nucleic acid copies which is necessary for reliable test results obeys binomial distribution or normal distribution, when the aliquot is taken from the original sample solution which includes at least two types of target nucleic acid copies; and comparing the determined requisite number of copies with the estimated number of copies of the target nucleic: acid in the amplification reaction solution.