Patent ID: 7732209

Claim:
A purified mutant hyperactive transposase of a mariner mobile genetic element (MGE), having at least one mutation by conservative substitution of at least one phosphorylatable residue in at least one phosphorylation site, said conservative substitution making said phosphorylation site non-phosphorylatable in vivo, said phosphorylatable residue being selected from the group consisting of: the following residues in sequence SEQ ID NO: 2: T11; T24; S28; T88; S99; S104; T135; S147; T154; S170; T181; S200; T216; T255; and S305, and corresponding residues in the sequence of a mariner MGE transposase aligned with said sequence SEQ ID NO: 2, said corresponding residues being selected from the group consisting of: a serine; and a threonine, wherein said conservative substitution does not comprise substituting said phosphorylatable residue with a residue selected from the group consisting of serine, threonine, aspartic acid, glutamic acid, and proline.