Patent ID: 7125702

Claim:
A process for the preparation of Angiotensin Converting Enzyme (ACE) inhibitory peptides from glycinin, said process comprising the steps of: (a) isolating glycinin from defatted soy flour; (b) dispersing a 10–25% solution of glycinin in a buffer; (c) hydrolyzing the mixture of glycinin and buffer with 2–4% w/v of one or more proteolytic enzymes; (d) incubating the mixture at 37–50° C. for a period of 16–18 hours; (e) arresting the hydrolysis reaction by adding trichloroacetic acid to the mixture; (f) centrifuging the mixture at 15–20,000 rpm for 30–40 minutes, at a temperature of about 4–8° C.; (g) obtaining from the centrifugation supernatant a mixture of protein hydrolysates containing polypeptides; and (h) separating and purifying from the mixture of protein hydrolysates peptide fractions having ACE inhibitory activity of 66%, 81% and 75%.