Patent ID: 8383065

Claim:
A test module for detecting target nucleic acid sequences in a fluid, the test module comprising: an outer casing having a receptacle for receiving the fluid containing the target nucleic acid sequences; electrochemiluminescent (ECL) probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer; electrodes for receiving an electrical pulse to excite the ECL luminophores; a detection photosensor for exposure to the photons emitted by the ECL luminophores; an array of hybridization chambers containing the ECL probes for different target nucleic acid sequences; and, a plurality of calibration sources each configured to provide a calibration emission, the calibration sources being distributed throughout the array of hybridization chambers such that each of the calibration sources are surrounded by a three-by-three square of the hybridization chambers.