Patent ID: 7399640

Claim:
A method for loading purified biomolecules from largely unpurified analytical biosample solutions to a sample support plate for a subsequent mass spectroscopic analysis with ionization by matrix-assisted laser desorption and ionization (MALDI), comprising the following steps (a) providing a sample support plate with three separate surface areas, including affinity adsorption surface areas each adjacent to a hydrophilic anchor surface area on an otherwise strongly hydrophobic plate surface, wherein the affinity adsorption areas are less hydrophobic than a lyophobic plate surface, but more hydrophobic than the hydrophilic anchor areas, (b) exposing the affinity adsorption areas to analytical sample solutions containing the biomolecules, thereby adsorbing the biomolecules to be analyzed, (c) washing the sample support plate comprising the adsorbed biomolecules with a solution that does not elute the adsorbed biomolecules, (d) desorbing the adsorbed biomolecules by an eluant solution which also contains matrix substance, and (e) drying the eluant solution, thereby forming matrix crystals containing desorbed biomolecules on the hydrophilic anchor areas.