Patent ID: 7297516

Claim:
A method of producing a recombinant polypeptide having an amino acid sequence that shares at least 80% identity with the amino acid sequence of ocean pout anti-freeze protein (AFP) type III HPLC 12 (SEQ ID NO: 27) and having an ice recrystallisation inhibition activity greater than that of a Winter flounder type I AFP polypeptide, which method comprises (1) providing a yeast comprising a DNA construct that is not present in the native yeast, the DNA construct comprising in the following order: (a) a promoter active in the yeast, (b) an ATG start codon; and (c) at least one nucleic acid sequence encoding said polypeptide, said nucleic acid sequence being in reading frame with the ATG start codon; (2) cultivating the yeast under conditions whereby expression of the polypeptide encoded by the nucleic acid sequence occurs or is induced; and optionally (3) recovering the recombinant polypeptide expressed from the nucleic acid sequence.