Patent ID: 8703903

Claim:
A method for quantifying an absolute amount of target isoallergens of an allergen of one species or an absolute amount of target homologous allergens of different species in an allergen sample, said method comprising: a) digesting said allergen sample with at least one enzyme to at least partially degrade at least one allergen in the allergen sample to obtain a mixture of peptides; b) combining said digested allergen sample with one or more synthetic allergen calibration standard peptides wherein said synthetic allergen calibration standard peptide is labeled with a first mass-modifying functionality, wherein said synthetic allergen calibration standard peptide consists of an amino acid sequence identical to a single peptide from each target isoallergen or a single peptide from each target homologous allergen resulting from digesting said allergen sample, and wherein said one or more synthetic allergen calibration standard peptide is unique for the target isoallergens or target homologous allergens to be detected; and c) correlating the signal of a known amount of said one or more synthetic allergen calibration standard peptides with an amount of the corresponding peptide in the digested allergen sample by mass spectrometry, wherein said correlation quantifies said absolute amount of target isoallergens or said absolute amount of target homologous allergens in said allergen sample, wherein said method does not quantify isoallergens or homologous allergens that are not target isoallergens or target homologous allergens.