Patent ID: 8129509

Claim:
A labeling molecule, for alkylating a chemical in a single-step reaction thereby providing the chemical with a detectable reporter molecule, comprising: 1) a spacer with a net positive charge and with affinity for nucleic acids selected from the group consisting of DNA minor groove binder and DNA major groove binder; 2) a reporter molecule selected from the group consisting of fluoresceins, rhodamines, lucifer yellow, 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene derivatives, malachite green, coumarins, dansyl derivatives, mansyl derivatives, dabsyl drivatives, NBD flouride, stillbenes, anthrocenes, rosamines, TNS chloride, eosins, ethidium bromide derivatives, thiazole orange derivatives, ethenoadenosines, cyanine derivatives, aconitine, Oregon Green, Cascade Blue, radioactive molecules, immunogenic molecules, haptens, biotin, oligosaccharides, and proteins; and, 3) an aromatic mustard reactive group.