Patent ID: 7674752

Claim:
An in situ method for producing an immobilized protein, domain or peptide array using wells of plates and a cell free system, wherein the method comprises the steps of: (a) providing DNA constructs that comprise (i) DNA sequences that encode proteins, domains or peptides, and (ii) DNA sequences that allow transcription and translation of the DNA sequences of (i) in a cell free system, wherein the encoded proteins, domains or peptides can be covalently or non-covalently attached to the wells of the plates; (b) distributing the DNA constructs in a gridded format into the wells of the plates that carry a ligand or reagent for immobilization of the proteins, domains or peptides encoded by the DNA sequences of (i); and (c) allowing transcribing and translating the DNA constructs of (b) to provide proteins, domains or peptides, in the gridded format, using the cell free system such that the translated protein products, domain products or peptide products are immobilized during production to the same wells in which the transcription and translation took place, thereby creating the protein, domain or peptide array.