Patent ID: 8476492

Claim:
A method of utilizing the pts gene and antisense ads gene to increase patchouli alcohol content in Artemisia annua L., said method comprising a) obtaining the complete open reading frame sequence of artemisinin biosynthesis pathway key enzyme ads gene of SEQ ID NO: 1, and the complete open reading frame sequence of the patchouli alcohol biosynthesis pathway key enzyme pts gene, set forth in SEQ ID NO: 2, linked to the plastid targeted signal peptide of the Arabidopsis tp gene as set forth in SEQ ID NO: 3; b) constructing plant expression vectors that contain the ads open reading frame in antisense orientation linked to transcriptional regulatory sequences, and the plastid targeted pts gene linked to transcriptional regulatory sequences; c) transforming Agrobacterium tumefaciens simultaneously with expression vectors containing the antisense ads and the plastid targeted pts gene to obtain Agrobacterium tumefaciens for transforming Artemisia annua L. plants; d) transforming Artemisia annua L. plants with the vector-transformed Agrobacterium tumefaciens obtained in Step c) and analyzing the transformed plants with PCR to obtain transgenic Artemisia annua L. plants; and e) determining the patchouli alcohol content in the transgenic Artemisia annua L. plants.