Patent ID: 8207093

Claim:
A method for producing an RNA-peptide fusion library, comprising the steps of: a) providing a population of RNA molecules generated from the DNA of SEQ ID NO: 34, each of which comprises a translation initiation sequence and a start codon operably linked to a protein coding sequence and each of which is operably linked to a peptide acceptor at the 3′ end of said protein coding sequence, wherein the peptide acceptor is selected from puromycin, phenylalanyl-adenosine (A-Phe), tyrosyl adenosine (A-Tyr), alanyl adenosine (A-Ala), phenylalanyl 3′ deoxy 3, amino adenosine, alanyl 3′ deoxy 3 amino adenosine, and tyrosyl 3′ deoxy 3′ amino adenosine, and wherein each of said RNA molecules further comprises a pause sequence which causes a ribosome to pause translation covalently bonded to the 3′ end of said RNA molecule; and b) in vitro translating said protein coding sequences and then incubating said translation reaction under monovalent or divalent cation salt conditions to produce a population of RNA-peptide fusions.