Patent ID: 8058514

Claim:
A method for producing a transformed maize, which comprises: (i) a co-culture step for culturing an Agrobacterium -inoculated immature maize embryo with a co-culture medium containing 3,6-dichloro-o-anisic acid, 4-amino-3,5,6-trichloropicolinic acid and/or 2,4,5-trichlorophenoxyacetic acid; said co-culture medium further comprising silver nitrate and/or copper sulfate; said co-culture medium excluding 2,4-dichloophenoxyacetic acid; wherein said Agrobacterium has a plasmid in which a resistance gene for a selective drug is inserted into the T-DNA; and (ii) a regeneration step for culturing the embryo obtained from (i) with a regeneration medium containing the selective drug to thereby induce regeneration and to produce a transformed plant, wherein said method does not comprise, between the co-culture step and the regeneration step, any selection step for culturing the co-cultured tissue with a medium containing an auxin and selective drug to select a transformant.