Patent ID: 7848889

Claim:
A method for allele assignments where assignments are made on the basis of subjecting a sample including target nucleic acid to a hybridization assay or capture-mediated elongation assay, or both, and where several polymorphic markers, present on alleles in the target nucleic acid are analyzed, the method comprising: determining the assay signal intensity pattern generated by probe-target interactions where each said intensity corresponds to the number of corresponding hybridization or elongation events, as applicable; binarizing said interaction pattern to generate a reaction pattern by designating as positive those signal intensities exceeding a pre-determined threshold and designating as negative those signal intensities not exceeding a pre-determined threshold; making allele assignments based on comparing and matching said reaction pattern with a similar reference reaction pattern representing combinations of two or more reference alleles, as listed in a reference collection of alleles for the target nucleic acid under consideration; designating as new alleles, those having reaction patterns which differ from reference reaction patterns representing combinations of two or more reference alleles by a pre-determined minimal dissimilarity; and displaying the allele assignments in a user readable form.