Patent ID: 8741644

Claim:
A method for producing human mast cells from human pluripotent stem cells with not less than 77% efficiency, comprising the steps of: (a) culturing human pluripotent stem cells under a condition suitable for promoting differentiation of the human pluripotent stem cells into hematopoietic progenitor cells expressing CD34; (b) suspension-culturing the cells obtained in step (a) in the presence of hematopoietic factors comprising TPO, Flt3 ligand, SCF, IL-6 and IL-3 for 5 to 7 days; and (c) suspension-culturing the cells obtained in step (b) in a serum-free medium containing hematopoietic factors comprising TPO, Flt3 ligand, SCF and IL-6 but not comprising IL-3, wherein human mast cells are produced with not less than 77% efficiency.