Patent ID: 6844158

Claim:
A method of reverse transcription-polymerase chain reaction (RT-PCR) comprising the steps of: preparing cell lysate of a target cell; transferring the cell lysate to an oligonucleotide-immobilized microplate having wells to which oligonucleotides are securely immobilized, said microplate having heat-stability for thermal cycles of PCR, said oligonucleotides having nucleic acid sequences complementary to mRNA of interest present in cell lysate; capturing mRNA by the oligonucleotides of the microplate; reverse transcribing the captured mRNA to cDNA by extending said oligonucleotides in the wells of the microplate; synthesizing DNA complementary to the cDNA to create double-stranded cDNA; and amplifying the double-stranded cDNA immobilized to the microplate in the microplate to create RT-PCR amplification products.