Patent ID: 7241589

Claim:
A method, for preparing androst-4-ene-3,17-dione (AD)/androsta-1,4-diene-3,17-dione (ADD), comprising the steps of: (a) heating sterols and an emulsifier respectively to dissolve completely and then mixing the dissolved sterols and emulsifier together to prepare a mixture wherein the emulsifier is selected from the group consisting of sucrose fatty acid ester, sorbitan fatty acid ester, polysorbate, polyglycerol fatty acid, propylene glycerol fatty acid and polyglycerine fatty acid ester; (b) placing the mixture in a water bath at 70–90° C. and stirring the mixture to obtain emulsified sterols; (c) adding the emulsified sterols to a culture media comprising Mycobacterium fortuitum EUG-119 (KCCM-10259) as a component of the media, and culturing Mycobacterium fortuitum EUG-119 (KCCM-10259) to produce androst-4-ene-3,17-dione (AD)/androsta-1,4-diene-3,17-dione (ADD); and (d) recovering said androst-4-ene-3,17-dione (AD)/androsta-1,4-diene-3,17-dione (ADD) from the culture.