Patent ID: 8193335

Claim:
A method for producing a reporter molecule comprising at least two different functional groups, said method comprising: (a) synthesizing said reporter molecule from a plurality of building blocks, wherein at least one building block comprises a first handle group which is selected from the group consisting of an alkyne group and a protected alkyne group, wherein at least one building block comprises a second handle group which is selected from the group consisting of an alkyne group and a protected alkyne group, and wherein said first handle group is different from said second handle group; (b) coupling a first reaction partner to said first handle group under conditions wherein said first handle group is reactive and said second handle group is not reactive, wherein said first reaction partner comprises a first functional group; and subsequently (c) coupling a second reaction partner to said second handle group wherein said second reaction partner comprises a second functional group and wherein said first functional group is different from said second functional group, wherein said reporter molecule is selected from the group consisting of nucleic acids and nucleic acid analogues.