Patent ID: 8088610

Claim:
A recombinant or isolated ketoreductase polypeptide capable of converting the substrate 2-[3-[3-[2-(7-chloro-2-quinolinyl)ethenyl]phenyl]-3-oxopropyl]benzoic acid methyl ester to the product (S,E)-methyl 2-(3-(3-(2-(7-chloroquinolin-2-yl)vinyl)phenyl)-3-hydroxypropyl)benzoate at a rate that is at least 1.5 times the rate of the polypeptide of SEQ ID NO:4, which comprises an amino acid sequence that is at least 90% identical to a reference sequence based on SEQ ID NO:2, 4 or 114 having the following features: residue corresponding to Y190 is proline; residue corresponding to L195 is arginine; and the residue corresponding to A202 in SEQ ID NO: 2 or L202 in SEQ ID NO: 4 or 114 is a tryptophan.