Patent ID: 7884201

Claim:
A method for separating and purifying RNA comprising: (1) passing a sample solution containing a nucleic acid through a nucleic acid-adsorbing porous membrane to adsorb the nucleic acid to the porous membrane; (2) passing a washing solution through the nucleic acid-adsorbing porous membrane to wash the porous membrane while adsorbing the nucleic acid; and (3) passing a recovering solution through the nucleic acid-adsorbing porous membrane to desorb the nucleic acid from the porous membrane, wherein the nucleic acid adsorbing porous membrane is a porous membrane capable of adsorbing a nucleic acid by interaction involving substantially no ionic bond, and wherein the sample solution containing the nucleic acid is obtained by a process for preparing a sample solution, the process comprising: (I) injecting a test sample containing: at least one of blood and leukocyte; and an anticoagulant to a container; (II) adding a hemolytic agent to the container and after adding the hemolytic agent, carrying out a centrifugation to obtain a leukocyte pellet; (III) adding a nucleic acid-solubilizing reagent to the leukocyte pellet to obtain a mixture solution; and (IV) adding a water-soluble organic solvent to the mixture solution to obtain the sample solution containing the nucleic acid.