Patent ID: 7728119

Claim:
A nucleotide primer set for LAMP amplification, used for detecting a genotype of a single-nucleotide polymorphism C677T of an MTHFR gene, wherein when a target nucleotide sequence has F3 region, F2 region and F1 region in turn from a 5′ terminal and B3c region, B2c region and B1c region in turn from a 3′ terminal, and when a primer set comprises an FIP primer having a sequence identical with that of the F2 region in a 3′ terminal side and a sequence complementary to the F1 region in a 5′ terminal side, an F3 primer having a sequence identical with that of the F3 region, a BIP primer having a sequence complementary to the B2c region in a 3′ terminal side and a sequence identical with that of the B1c region in a 5′ terminal side, and a B3 primer having a sequence complementary to the B3c region, the primer set comprises: an FIP and BIP primer set selected from the group consisting of: SEQ ID NO: 1 and SEQ ID NO: 3; SEQ ID NO: 1 and SEQ ID NO: 4; SEQ ID NO: 1 and SEQ ID NO: 5; SEQ ID NO: 1 and SEQ ID NO: 6; SEQ ID NO: 1 and SEQ ID NO: 7; SEQ ID NO: 9 and SEQ ID NO: 3; SEQ ID NO: 9 and SEQ ID NO: 4; and SEQ ID NO: 9 and SEQ ID NO: 5; an F3 primer binding to a region within 60 bases from the 5′ terminal of a F2c region of a complementary nucleotide sequence of the target nucleotide sequence, wherein the F2c region is complementary to the F2 region; and a B3 primer binding to a region within 60 bases from the 3′ terminal of the B2c region of the target nucleotide sequence.