Patent ID: 8186044

Claim:
A method of manufacturing a plurality of electrochemical sensors, the method comprising steps of: (a) forming a plurality of working electrodes on a first substrate; (b) forming a plurality of counter electrodes on a second substrate; (c) forming a plurality of indicator electrodes on one of the first substrate and the second substrate; (d) disposing a spacer layer on either said first or second substrate; (e) overlaying the first substrate and the second substrate to form a layered structure, thereby creating a sample chamber region between the first substrate and the second substrate; and (f) separating a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, at least one indicator electrode, at least one sample inlet port and at least one sample chamber region, such that the sample inlet port and the indicator electrode flank the working electrode and the counter electrode such that the indicator electrode is positioned to indicate when the sample chamber is full.