Patent ID: 7582457

Claim:
A process for the bio-production of 1,3-propanediol comprising: (i) contacting a transformed host cell with at least one fermentable carbon source and an effective amount of coenzyme B 12 precursor whereby 1,3-propanediol is produced, the transformed host cell comprising: (a) at least one copy of genes encoding a protein having a glycerol dehydratase activity or a diol dehydratase activity selected from the group consisting of Klebsiella pneumoniae dhaB1-3 as shown in SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6; (b) at least one copy of a gene encoding a protein having a 1,3-propanediol oxidoreductase activity of Klebsiella pneumoniae dhaT as shown in SEQ ID NO:7; and (c) one copy of a gene encoding a protein having a cob(I)alamin adenosyltransferase activity selected from the group consisting of Escherichia coil btuR as shown in SEQ ID NO:1, Salmonella typhimurium cobA as shown in SEQ ID NO:2, and Pseudomonas denitrificans cobO as shown in SEQ ID NO:3; wherein the genes of (i)(a)-(i)(c) are heterologous to the host cell and express their respective gene products in active form, and (ii) recovering the 1,3-propanediol produced from step (i).