Patent ID: 8791258

Claim:
A pro-fluorescent compound having the formula: wherein X and Z are members independently selected from CR 5 R 6 , C(O), NR 5 , O and substituted or unsubstituted heterocycloalkyl wherein R 5 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; R 6 is a member selected from CN, COR′, OR 8 , substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl wherein R 7 is a member selected from OR 9 and NR 9 R 10 wherein R 9 and R 10 are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; R 8 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and substituted or unsubstituted heterocycloalkyl; r is an integer selected from 1, 2 and 3; R 1 , R 2 , R 3 and R 4 are members independently selected from H, substituted or unsubstituted alkyl, and substituted or unsubstituted heteroalkyl; L 1 is a linker which is a member selected from a bond, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl; and X 1 is a member selected from a charged group, NR 11 R 12 , OR 13 , substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl wherein R 11 , R 12 and R 13 are members independently selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl.