Patent ID: 8592642

Claim:
A method for evaluating an effect of a transplanted tumor on a mouse tissue, or cultured cells derived from the tissue, said method comprising the steps of: (1) providing a Dao 1 +/+ mouse and a Dao 1 −/− mouse, (2) exposing a tissue of the Dao 1 +/+ and Dao 1 −/− mice, or cultured tissue cells derived from the tissue to the transplanted tumor, and (3) comparing changes detected in the tissue or cultured tissue cells derived from the tissue of the Dao 1 +/+ and Dao 1 −/− mice exposed to the transplanted tumor with tissue or cultured tissue cells derived from the tissue of the Dao 1 +/+ and Dao 1 −/− mice that were not exposed to the transplanted tumor, wherein the mice of step (1) are provided by the following steps: (a) identifying a Dao 1 +/+ , +/− and/or −/− mouse individually, (b) extracting chromosomal DNA from the mouse (a), (c) obtaining an amplified DNA fragment by amplifying the region covering the seventh exon of the Dao 1 gene from the extracted chromosomal DNA, (d) digesting the amplified DNA fragment with a HpaII restriction enzyme and examining whether the amplified DNA fragment is cleaved or not, (e) determining a genotype of Dao 1 +/+ , +/− and/or −/− ; and (f) discriminating the Dao 1 +/+ mouse from the Dao −/− mouse.