Patent ID: 8293876

Claim:
A method for purifying recombinant synthetic Lyme antigen (rSLA) comprising the steps of: (i) subjecting a homogenate of cells containing the rSLA to microdiafiltration comprising the steps of: (a) concentrating said homogenate by microfiltration; (b) washing the biomass by microdiafiltration, thereby obtaining a microdiafiltrate-1 and a microdiaretentate-1; (c) extracting the rSLA from said microdiaretentate-1 by microdiafiltration using a buffer containing a detergent thereby obtaining a microdiafiltrate-2 and a microdiaretentate-2; (ii) subjecting said microdiafiltrate-2 containing the extracted rSLA to ion exchange chromatography, the eluent thereof containing the purified rSLA; (iii) subjecting the eluent obtained from the ion exchange chromatography in step (ii) to anion exchange filtration for residual endotoxin removal; and (iv) subjecting the such obtained protein solution containing the rSLA to hydroxyapatite (HA) column chromatography.