Patent ID: 8187438

Claim:
An improved method for the separation and detection of a sample, which method comprises (a) separating said sample by gel electrophoresis; and (b) blotting and detecting separated sample; the improvement comprising using an electrophoretic gel provided in direct contact with a non-adherent polymer film for electrophoresis and subsequent blotting, wherein the polymer film is selected from a polymer having the following formula: wherein n=0-100 000 m=0-100 000 R1, R2, R3 and R4=H, F, Cl, Br, I, methyl groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethyl, ethenyl, propyl, isopropyl, propenyl, butyl, branched butyl, butenyl, cyclobutyl, pentyl, branched pentyl, pentenyl, cyclopentyl, hexyl, branched hexyl, cyclohexyl; X, Y=methylene groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethylene, ethenylene, propylene, isopropylene, propenylene, butylene, branched butylene, butenylene; Y can optionally be absent, wherein the format of the gel is at the most 150×150×1 mm.