Patent ID: 7316912

Claim:
A method of determining whether a candidate compound is a modulator of tyrosylprotein sulfotransferase activity comprising: (a) contacting a candidate compound with a purified or recombinant tyrosylprotein sulfotransferase under conditions suitable for causing transfer of sulfate from a sulfate donor to a tyrosine of a protein, polypeptide or peptide, wherein said tyrosylprotein sulfotransferase is encoded by a polynucleotide selected from the group consisting of: (i) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 198-1331 of SEQ ID NO:6; (ii) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 402-1199 of SEQ ID NO:6; (iii) a polynucleotide encoding a polypeptide comprising the amino acid sequence encoded by nucleotides 198-1331 of SEQ ID NO:6; and (iv) a polynucleotide encoding a polypeptide comprising the amino acid sequence encoded by nucleotides 402-1199 of SEQ ID NO:6, and wherein the polynucleotides of (i) and (ii) hybridize under hybridization conditions comprising: incubation at 68° C. overnight followed by washing twice with two times SSC, 0.1% SDS at 22° C., and washing twice with 0.1 times SSC, 0.1% SDS at 50° C.; and (b) determining whether said candidate compound modulates the activity of said tyrosylprotein sulfotransferase, wherein said determining step further comprises measuring a level of tyrosylprotein sulfotransferase activity, wherein a level of tyrosylprotein sulfotransferase activity in the presence of said candidate compound that is modulated relative to tyrosylprotein sulfotransferase activity in the absence of said candidate compound is indicative of tyrosylprotein sulfotransferase modulatory activity of said candidate compound.