Patent ID: 8361797

Claim:
A method for the in vitro culture of a myeloma cell line, said method comprising: (a) inoculating a culture medium with the myeloma cell line, said medium being capable of supporting the growth of said myeloma cell line and comprising iron at concentrations in the medium of from 0.064 mg/L to 1.6 mg/L, wherein said medium does not contain any of a transferrin, a lipophilic chelator, a synthetic nitrogen-containing chelator and a lipophilic synthetic nitrogen-containing chelator; and (b) growing of the inoculated culture medium under appropriate conditions and using agitated suspension culture, and wherein the source of iron is a soluble iron compound selected from the group consisting of ferric ammonium citrate, ferric ammonium oxalate, ferric ammonium fumarate, ferric ammonium malate and ferric ammonium succinate.