Patent ID: 7915016

Claim:
A method for a combined lysis and cDNA synthesis of RNA from a contamination-free single cell or cells of a tissue sample after laser microdissection, comprising the steps of: (a) performing laser microdissection to prepare a single cell or cells from a tissue sample; (b) transferring the microdissected single cell or cells contact-free to a sterile reaction tube with a lid by automatically laser pressure catapulting the micro dissected single cell or cells directly into the sterile reaction tube with a lid; (c) adding a sterile lysis cDNA synthesis reaction buffer comprising DTT, Poly-I-Carrier RNA, Nonidet P40, dNTPs, random hexamer Primer and, an amount of an RNase inhibitor effective to provide the buffer RNase free to said sterile reaction tube with a lid and lysing the microdissected cells; (d) following step (c), reverse transcribing RNA from the lysed cells in the same sterile lysis/cDNA synthesis reaction buffer in the same sterile reaction tube to produce cDNA; and (e) detecting the cDNA or detecting and characterizing the cDNA obtained according to step (d) by carrying out direct PCR without any further purification or other additional steps.