Patent ID: 7666612

Claim:
A method for detecting and/or manipulating a protein of interest, which comprises contacting a fusion protein comprising a protein of interest consisting of at least 12 amino acids and up to 2,000 amino acids and an acyl carrier protein (ACP) or a fragment thereof containing a serine residue to which a phosphopantetheine moiety of coenzyme A (CoA) is attachable, and which retains the function to accept such phosphopantetheine moiety with a coenzyme A (CoA) type substrate, wherein the phosphopantetheinyl moiety of the CoA carries a label as a ligand connected via a linker which can be transferred to the ACP fusion protein, and with a holo-acyl carrier protein synthase (ACPS) or a homologue thereof selected from the group consisting of EntD, Sfp, Psf-1, Gsp, LYS5, Bli, Lpa14, and NshC, so that the ACPS transfers the label to the fusion protein, and detecting and/or further manipulating the labeled fusion protein obtained, using the label in a system designed for detecting and/or manipulating the label.