Patent ID: 6921653

Claim:
A method of obtaining a triclinic crystal composition of the bacterial membrane associated UDP-glycosyltransferase of SEQ ID NO: 1 (MurG protein), said method consisting of: (a) preparing a solution containing 10 mg/ml of MurG protein in 20 mM Tris-HCl buffer, pH 7.9, 150 mM NaCl, 50 mM EDTA, and three molar equivalent of UDP-GlcNAc to the MurG protein; and (b) growing a triclinic crystal at room temperature using the hanging drop-method by mixing equal volumes of the protein solution of (a) with a reservoir solution consisting of 0.1 M NaMES, pH 6.5, 0.96 M ammonium sulfate, 0.4% Triton X-100, and 10 mM DTT.