Patent ID: 8889353

Claim:
Method for monitoring the bisulfite-mediated conversion of DNA during DNA methylation analysis, comprising: (a) providing a sample DNA to be analyzed and at least one DNA reporter molecule, wherein the at least one DNA reporter molecule comprises: (i) in its nucleotide sequence at least one unmethylated cytosine residue; and (ii) at least one label; and wherein the sample DNA and the at least one DNA reporter molecule are provided in spatially separated reaction compartments that are in fluid connection with each other; (b) adding a bisulfite salt to the spatially separated reaction compartments, thus mediating the conversion of the unmethylated cytosine residues comprised in the nucleotide sequences of the sample DNA and the at least one DNA reporter molecule into uridine residues; (c) adding an uracil-DNA-glycosylase to the at least one DNA reporter molecule, thus mediating the removal of the uracil bases obtained in step (b) from the DNA backbone; (d) fragmentation of the DNA obtained in step (c) by heat treatment; and (e) detecting the at least one label released from the at least one DNA reporter molecule during step (d).