Patent ID: 7914769

Claim:
A method of identifying a compound that inhibits an activity of the human papillomavirus (HPV) 16 E6 protein, the method comprising: (a) providing a compound of formula (I): wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 and R 7 are each, independently, selected from the group consisting of hydrogen, alkyl, cycloalkyl, haloalkyl, aryl, heteroaryl, aralkyl, heteroaralkyl, heterocyclyl, alkenyl, alkynyl, cycloalkenyl, heterocycloalkenyl, alkoxy, haloalkoxy, halo, hydroxy, carboxy, carboxylate, formyl, acyl, cyano, nitro, amino, alkyl amino, SO 3 H, sulfate, phosphate, methylenedioxy, ethylenedioxy, oxo, thioxo, imino, S(O) n alkyl, S(O) n aryl, S(O) n heteroaryl, S(O) n heterocyclyl, amine, ester, amide, and sulfonamide; Ar is selected from the group consisting of aryl and heteroaryl; R 8 is selected from the group consisting of hydrogen and C 1 -C 10 alkyl; and X is selected from the group consisting of S and O; and (b) determining whether the compound alters the level of binding between E6 and a protein containing an E6 binding motif, wherein a decrease in the level of binding between the E6 protein and the protein containing an E6 binding motif in the presence of the compound compared to the level binding in the absence of the compound identifies the compound as a compound that inhibits an activity of the HPV 16 E6 protein.