Patent ID: 7374913

Claim:
A method for synthesizing a polynucleotide comprising the steps of mixing the following elements 1 to 5, and incubating under conditions that allow template-dependent complementary strand synthesis using the DNA polymerase in 4: 1: a template polynucleotide that: (a) has a target nucleotide sequence comprising at least one set of complementary nucleotide sequences, (b) forms a loop capable of base pairing when the complementary nucleotide sequence of (a) hybridizes, (c) forms a loop by the annealing of its 3′-end to itself, and (d) whose 3′-end annealed to itself can be a starting point for complementary strand synthesis using itself as template; 2: at least two types of primers providing staffing points for complementary strand synthesis at different locations on the template polynucleotide loop; 3: at least one type of primer providing a staffing point for complementary strand synthesis at a location different from the primers of 2 in a loop formed by the template polynucleotide and/or an elongation product produced by the annealing of the primers of 2 to the template polynucleotide; 4: a DNA polymerase catalyzing complementary strand synthesis accompanying strand displacement; and, 5: a substrate for complementary strand synthesis.