Patent ID: 7406385

Claim:
A software-implemented method for consensus basecall analysis used for sequence identification and quality assessment, the method comprising: receiving input sequence data as nucleotide base assembly information for at least one sequence fragment wherein the assembly information comprises a plurality of putative basecalls spanning at least a portion of the sample sequence, wherein each putative basecall comprises a pure-basecall indicative of a single nucleotide base, a mixed-basecall indicative of two or more nucleotide bases, or a gap indicative of a region lacking an identified nucleotide base, the assembly information further comprising a plurality of per-base quality values associated with the plurality of putative basecalls; performing a recalling operation on selected mixed-basecalls determined on the basis of quality values associated with the selected mixed-basecalls; performing a consensus calling operation wherein the putative basecalls are aligned to generate the consensus sequence representative of a predicted base sequence for the sample sequence; performing a consensus quality value determination wherein for each base of the consensus sequence a consensus quality value is calculated using the per-base quality values associated with that base, the consensus quality value reflective of the confidence in the consensus basecall for that base; and outputting the consensus quality values for the input sequence data wherein the consensus quality values reflect the confidence in sequence identification of the consensus basecall analysis.