Patent ID: 6949349

Claim:
A method for determining the relative affinity for binding to an IGFBP of a peptide that competes with an IGF for binding to the IGFBP, which peptide is produced by a phagemid clone present in a phage-displayed library, which method comprises (a) incubating a phagemid clone displaying the peptide with the IGF in the presence of the IGFBP at high and low concentrations of the phagemid clone, (b) and measuring the degree to which binding of the phagemid clone to the IGFBP is inhibited by the IGF at each phagemid concentration, wherein the phagemid clone the binding of which is inhibited only at low phagemid concentrations has a higher affinity for the IGFBP than a phagemid clone the binding of which is inhibited at both high and low phagemid concentrations.