Patent ID: 8367120

Claim:
A method for producing a stable, non-toxic, antimicrobial electrolyzed saline solution comprising: preparing a saline solution having a saline concentration within a range from approximately 0.15% to approximately 1.0% by weight; inserting within the saline solution an inert anode and a spaced apart corresponding inert cathode associated with a power source; regulating the temperature of the saline solution to maintain a solution temperature sufficient to prevent production of chlorates and regulate relative concentrations of resulting components during electrolysis; circulating the saline solution to maintain a flow of the saline solution between the anode and cathode; applying an effective voltage potential selected from within a voltage range of zero volts to approximately thirty volts between the cathode and the anode sufficient to produce a mixture of chemically reduced and oxidized molecules, the mixture including hypochlorous acid, hypochlorites, dissolved oxygen chlorine, hydrogen gases, hydrogen peroxide, hydrogen, hypochloride and corresponding amounts of superoxides, ozone, activated hydrogen, chloride ions, hydroxides, singlet oxygen, and other forms of reactive oxygen species, wherein the total combination of hypochlorous acid, hypochlorites, and dissolved oxygen chlorine is at a concentration between 1 to 200 ppm, wherein the total combination of hydrogen gases, hydrogen peroxide, hydrogen, hypochloride, superoxides, and ozone is at a concentration from 1 to 50 ppm, wherein total reactive oxygen species in the balanced mixture is between 0.05 to 50 ppm; and utilizing one or more techniques to achieve desired electrolysis efficiencies and stable specie compositions containing stable reactive oxygen species while preventing production of chlorates, the one or more techniques including one or more of the following: electron and proton donation; ion and dissolved-gas transport; adjusting a temperature of the saline solution; adjusting anode and cathode spacing; adjusting a saline solution circulation rate; and optimization of the effective voltage; mirroring the mixture of chemically reduced and oxidized species in the electrolyzed saline solution to the reduced species and reactive oxygen species found in a known biological system by measuring concentrations of reactive oxygen species in the electrolyzed saline solution using a fluoro spectrometer and at least one fluorescent dye selected from R-phycoerytherin, hydroxyphenyl fluorescein, and aminophenyl fluorescein; and producing the stable, non-toxic, antimicrobial electrolyzed saline solution.