Patent ID: 7176352

Claim:
A method for the stable genetic transformation of Lemnaceae whole plants, plant tissue or callus, which comprises: brining the Lemnaceae whole plant, plant tissue or callus into contact with Agrobacterium cells containing a transforming DNA molecule; and incubating the Lemnaceae whole plant, plant tissue or callus with the Agrobacterium cells, whereby cells in said whole plant, plant tissue or callus become stably transformed with said DNA, wherein the Agrobacterium cells are brought into contact, prior to or during the transformation method, with a booster medium that enhances the Agrobacterium cells' virulence, said booster medium comprising a fresh cell suspension of dicotyledonous plants or comprising Lemnaceae plant extracts, and further comprising caffeine at a concentration of 100–500 mg per liter of medium.