Patent ID: 6861236

Claim:
A method for producing a thioether bridge containing polypeptide of a non-Gram positive bacterium origin in a host cell, said method comprising: selecting a host cell having a nucleic acid molecule comprising: a first nucleic acid fragment encoding a leader peptide; a second nucleic acid fragment encoding a polypeptide comprising a sequence selected from the group of sequences consisting of Ser-Cys, Ser-Xaa 1-20 -Cys (SEQ ID NO: 310), Thr-Cys, and Thr-Xaa 1-20 -Cys (SEQ ID NO: 311), wherein Xaa is any amino acid and the polypeptide is of the non-Gram positive bacterium origin; wherein the first and second nucleic acid fragments are within the same open reading frame as the nucleic acid molecule; and wherein the leader peptide acts as a translocation signal sequence and a recognition signal such that the thioether bridge formation may occur; selecting the host cell for the presence of the transporter protein LanT or a functional equivalent thereof having LanT activity; translating the nucleic acid molecule, thus producing the leader peptide and the polypeptide comprising the sequence selected from the group of sequences consisting of Ser-Cys, Ser-Xaa 1-20 -Cys (SEQ ID NO: 310), Thr-Cys, and Thr-Xaa 1-20 -Cys (SEQ ID NO: 311); and harvesting the polypeptide containing the thioether bridge from a medium of the host cell.