Patent ID: 8030462

Claim:
An immunostimulatory oligonucleotide compound comprising a sequence of formula (III): (III) 5′-Um . . . U1-X1-X2-Y-Z-X3-X4-D1 . . . Dm-3′ wherein: Y is selected from the group consisting of 5-hydroxycytosine, 5-hydroxymethylcytosine, N4-alkylcytosine and 4-thiouracil; Z is selected from the group consisting of guanosine, 2′-deoxy-guanosine, 7-deazaguanosine and 6-thioguanosine; each X is independently selected from the group consisting of a naturally occurring nucleoside, C3-alkyl linker, 2-aminobutyl-1,3-propanediol linker, β-L-deoxynucleoside, 1′,2′-dideoxyribose, C3-linker, Spacer 18, 3′-deoxynucleoside, 2′-O-propargyl-ribonucleoside, Spacer 9 and 2′-5′ linkage; wherein Um-U1 represents an upstream potentiation domain, where each U independently is a naturally occurring nucleoside or an immunostimulatory moiety; wherein D1-Dm represents a downstream potentiation domain, where each D independently is a naturally occurring nucleoside or an immunostimulatory moiety; and m, at each occurrence, represents a number from 0 to 30; and wherein at least one X, U, or D is selected from the group consisting of C3-alkyl linker, 2-aminobutyl-1,3-propanediol linker, β-L-deoxynucleoside, 1′, 2′-dideoxyribose, C3-linker, Spacer 18, 3′-deoxynucleoside, 2′-O-propargyl-ribonucleoside, Spacer 9 and 2′-5′ linkage.