Patent ID: 7125972

Claim:
A solid carrier comprising at least two single stranded peptide nucleic acid probes of different base sequence covalently bound to the solid carrier at separate and distinct regions; wherein the probes have the general formula (I): wherein: n is at least 2, each of L 1 –L n is independently selected from the group consisting of hydrogen, hydroxy, (C 1 –C 4 ) alkanoyl, naturally occurring nucleobases, non-naturally occurring nucleobases, aromatic moieties, DNA intercalators, nucleobase-binding groups and reporter ligands, at least one of L 1 –L n being a naturally occurring nucleobase, a non-naturally occurring nucleobase, a DNA intercalator, or a nucleobase-binding group; each of A 1 through A n is a single bond, a methylene group or a group of formula (IIa) or (IIb): wherein in (IIa) or (IIb): X is O, S, Se, NR 3 , CH 2 or C(CH 3 ) 2 ; Y is a single bond, O, S or NR 4 ; each of p and q is an integer from 1 to 5, the sum p+q being not more than 10; each of r and ss is zero or an integer from 1 to 5, the sum r+ss being not more than 10; each R 1 and R 2 is independently selected from the group consisting of hydrogen, (C 1 –C 4 ) alkyl which may be hydroxy- or alkoxy- or alkylthio-substituted, hydroxy, alkoxy, alkylthio, amino and halogen; and each R 3 and R 4 is independently selected from the group consisting of hydrogen, (C 1 –C 4 )alkyl, hydroxy- or alkoxy- or alkylthio-substituted (C 1 –C 4 )alkyl, hydroxy, alkoxy, alkylthio and amino; and wherein in formula (1) each of B 1 –B n is N or R 3 N + , where R 3 is as defined above; each of C 1 –C n is CR 6 R 7 , CHR 6 CHR 7 or CR 6 R 7 CH 2 , wherein R 6 is hydrogen and R 7 is selected from the group consisting of the side chains of naturally occurring alpha amino acids, or R 6 and R 7 are independently selected from the group consisting of hydrogen, (C 2 –C 6 ) alkyl, aryl, aralkyl, heteroaryl, hydroxy, (C 1 –C 6 ) alkoxy, (C 1 –C 6 ) alkylthio, NR 3 R 4 and SR 5 , where R 3 and R 4 are as defined above, and R 5 is hydrogen, (C 1 –C 6 )alkyl, hydroxy-, alkoxy-, or alkylthio- substituted (C 1 –C 6 )alkyl, or R 6 and R 7 taken together complete an alicyclic or heterocyclic system; each of D 1 –D n is CR 6 R 7 , CH 2 CR 6 R 7 or CHR 6 CHR 7 , where R 6 and R 7 are as defined above; each of G 1 –G n-1 is in any orientation, wherein R 3 is as defined above; Q is —CO 2 H, —CONR′R″, —SO 3 H or SO 2 NR′R″ or an activated derivative of —CO 2 H, —SO 3 H; and I is —NHR′″R″″ or —NR′″C(O)R″″, where R′, R″, R′″ and R″″ are independently selected from the group consisting of hydrogen, alkyl, amino protecting groups, reporter ligands, intercalators, chelators, peptides, proteins, carbohydrates, lipids, steroids, oligonucleotides and soluble and non-soluble polymers, wherein C and D can optionally be CHR 6 (CH 2 ) sss CHR 7 where sss is from 0 to 2 and wherein the solid carrier is manufactured by binding fully formed peptide nucleic acid probes to different binding regions on the surface of the solid carrier.