Patent ID: 8202709

Claim:
A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock, the process comprising: (i) partially hydrolyzing an aqueous slurry of the pretreated lignocellulosic feedstock with an enzyme composition to produce a hydrolyzed slurry comprising glucose, glucose oligomers or a combination thereof, and unhydrolyzed fiber solids comprising cellulose and lignin, said enzyme composition comprising cellulase enzymes and at least one β-glucosidase enzyme comprising a cellulase binding domain that binds said β-glucosidase enzyme to said pretreated lignocellulosic feedstock; (ii) separating the unhydrolyzed fiber solids from the aqueous phase to produce separated fiber solids, wherein the cellulase enzymes and the at least one β-glucosidase enzyme comprising a cellulose binding domain bind to the unhydrolyzed separated fiber solids; (iii) re-suspending the unhydrolyzed separated fiber solids in an aqueous solution to produce a re-suspended slurry; and (iv) continuing the hydrolysis of the re-suspended slurry to produce the hydrolysis product comprising glucose.