Patent ID: 8849578

Claim:
An in silico method of determining a relative aggregation rate indicator, said relative aggregation rate indicator predicting a rate of aggregation of a second polypeptide based upon its amino acid seqeuence in comparison to a rate of aggregation of a reference polypeptide, said second polypeptide comprising one or more amino acid modification(s) when compared to the reference polypeptide, the method comprising using one or more processors to: determine a difference in hydrophobicity between said second polypeptide and said reference polypeptide; determine a difference in a secondary structure propensity between said second polypeptide and said reference polypeptide; determine a difference in charge between said second polypeptide and said reference polypeptide; form a weighted combination of said difference in hydrophobicity, said difference in secondary structure propensity, and said difference in charge to determine said relative aggregation rate indicator by summing each said difference multiplied by a scaling factor; wherein said scaling factors used in said weighted combination are selected in response to data on said reference polypeptide.