Patent ID: 7691996

Claim:
A chimeric antisense oligonucleotide comprising in the following order: a 5′ terminus; a first region comprising a non-RNAse H-activating region; a second region comprising an RNAse H activating region; a third region comprising a non-RNAse H-activating region; and a 3′ terminus; wherein said first non-RNAse H-activating region comprises 5′→3′-linked nucleotides and said third non-RNAse H-activating region comprises 5′→3′-linked nucleotides; wherein the combined number of nucleotides from said first and third non-RNAse H-activating regions is from 11 to 59 and said 5′→3′-linked nucleotides are independently selected from the group consisting of 2′ modified phosphodiester nueleotides and 2′-modified P-alkoxyphosphotriester nucleotides; wherein said RNAse H-activating region comprises between three and ten contiguous phosphorothioate-linked deoxyribonucleotides; and wherein the 3′ terminus of said oligonucleotide is selected from the group consisting of: an inverted deoxyribonucleotide, a contiguous stretch of one to three phosphorothioate 2′-modified ribonulceotides, a biotin group, and a P-alkyloxyphosphotriester nucleotide.