Patent ID: 8268586

Claim:
A method for producing a polypeptide having biological activity in a bacterial host cell, comprising: (a) cultivating the bacterial host cell in a medium conducive for production of the polypeptide having biological activity, wherein the bacterial host cell comprises one or more copies of a nucleic acid construct comprising a promoter region operably linked to a polynucleotide sequence encoding the polypeptide having biological activity and a modified mRNA processing/stabilizing sequence located downstream of the promoter region and upstream of the ribosome binding site of the polynucleotide sequence encoding the polypeptide having biological activity, wherein the modified mRNA processing/stabilizing sequence is a cryIIIA mRNA processinq/stabilizing sequence comprising at least one additional copy of a Shine-Dalgamo sequence or a SP82 mRNA processing/stabilizing sequence comprising at least one additional copy of a Shine-Dalgamo sequence; and wherein the modified mRNA processing/stabilizing sequence promotes higher expression of the polynucleotide sequence compared to an unmodified mRNA processing/stabilizing sequence; and (b) isolating the polypeptide having biological activity from the cultivation medium.