Patent ID: 8580555

Claim:
An engineered halohydrin dehalogenase (HHDH) comprising an amino acid sequence at least 90% identical to SEQ ID NO:244 and has at least one residue difference as compared to SEQ ID NO:244 selected from: X12 is M, or S; X34 is G; X51 is P; X81 is E, F, M, W, or Y; X82 is C, G, K, L, M, N, or W; X83 is C or E; X84 is D; X85 is V; X86 is A or H; X110 is S, X131 is A; X134 is C, F, I, K, L, M, or V; X139 is G, I, L, M, P, S, T, or V; X142 is C, M or N; X146 is P; X167 is H; X175 is C, G, I, L, M, N, or V; X176 is F, M, Q, or T; X177 is L, S or T; X178 is T; X182 is S; X186 is H; X187 is G, I, or S; X201 is R; X232 is S, X242 is A; X243 is C or L; X245 is S; X246 is S; X247 is N; X248 is V; X249 is A, G, H, I, or S; X252 is C; and X254 is N.