Patent ID: 8440440

Claim:
A method for recovering stromal vascular fraction cells from blood vessels contained in or proximate to human adipose tissue comprising treating about 45-60 cc of adipose tissue comprising fat cells, blood vessels, and a stromal vascular fraction in a 60 cc tube with ultrasonic cavitation for about 10 minutes using a 14 mm ultrasonic rod, wherein ultrasonic cavitation is effected by slowly increasing cycle and amplitude once the ultrasonic rod is submerged into the tube containing the adipose tissue, wherein after 5 minutes, the ultrasonic process is stopped and the ultrasonic rod is adjusted up in the tube and continued for the 5 minutes, and wherein the fat cells and blood vessels are exploded, thereby releasing substantial numbers of intact stromal vascular fraction cells while substantially maintaining the viability of the cells constituting the stromal vascular fraction.