Patent ID: 7491496

Claim:
A method of manufacturing a biosensor, comprising: applying a solution to an Au solid support, the solution having a plurality of probe molecules, a plurality of spacer molecules, and at least one kind of a divalent cation that includes a CaCl 2 , each of the plurality of probe molecules including a nucleic acid, each of the plurality of spacer molecules not including a nucleic acid and not covalently linked to the plurality of probe molecules, and the CaCl2 at a total concentration in a range of 0.1 mM-20 mM; and adsorbing the plurality of probe molecules and the plurality of spacer molecules onto the Au solid support, a surface concentration of the plurality of probe molecules being at least 1×10 8 and at most 1×10 16 per square centimeter, and the plurality of spacer molecules being positioned between the plurality of probe molecules.