Patent ID: 8546645

Claim:
A method for producing a transgenic plant, comprising: (a) co-transforming plant cells with: i. a first nucleic acid construct, said first nucleic acid construct comprising a nucleotide sequence of at least contiguous 100 nucleotides, said nucleotide sequence possessing at least 50% sequence identity over its entire length to a native ribosomal DNA (rDNA) sequence of said plant cells; and ii. a second nucleic acid construct, said second nucleic acid construct comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant cells; thereby obtaining transgenic plant cells; (b) regenerating a plurality of transgenic plants from said transgenic plant cells; and (c) selecting from said plurality of transgenic plants a transgenic plant wherein said second nucleic acid construct is amplified, and a plurality of said second nucleic constructs are stably integrated into or adjacent to the native rDNA of said plant cell in sufficiently close proximity to one another that they segregate together as a single genetic locus.