Patent ID: 7160544

Claim:
A method of vaccinating a vertebrate species, comprising administering to a vertebrate species a vaccine comprising a composition prepared by: (A) forming a water-in-oil emulsion comprising (a) water, (b) an alginate, (c) an oil, (d) an antigen, and (e) a surfactant composition comprising at least one of (i) a cellulose ether and at least one nonionic surfactant and (ii) a poly(ethylene oxide)-poly(propylene oxide)-poly (ethylene oxide) triblock copolymer surfactant and at least one nonionic surfactant; (B) crosslinking the alginate in the emulsion of step (A) with at least two different cations selected from the group consisting of aluminum, barium, calcium, manganese, strontium, and zinc, to form antigen-containing crosslinked alginate microparticles; and (C) harvesting the microparticles of step (B).