Patent ID: 6858414

Claim:
A process for isolating deferoxamine B mesylate from a source material containing deferoxamine B produced by a microbiological process comprising the steps of: a) adsorbing deferoxamine B onto an adsorption resin by contacting the source material with the adsorption resin, b) obtaining an eluent containing deferoxamine B by eluting the adsorption resin having deferoxamine B adsorbed thereon with a mixture of water and a water-soluble organic elution solvent, c) adjusting the pH of the eluent to between about 8.6 and 10.5 with a basic ion exchange resin or an alkaline solution, or both, d) crystallizing deferoxamine B free base from the pH-adjusted eluent by addition of a deferoxamine B free base anti-solvent, optionally after partial concentration of the eluent to a deferoxamine B concentration of not more than 150 g L −1 , e) suspending the deferoxamine B free base in a mixed solvent comprising a deferoxamine B mesylate anti-solvent and a deferoxamine B mesylate solvent selected from the group consisting of water and methanol, f) dissolving the deferoxamine B free base in the mixed solvent by contacting the suspension with methanesulfonic acid, and g) precipitating deferoxamine B mesylate.