Patent ID: 8771998

Claim:
A method for the fermentative production of gamma-aminobutyric acid (GABA), comprising cultivating a recombinant microorganism derived from a parent microorganism having the ability to produce glutamate and additionally having the ability to express a heterologous glutamate decarboxylase (E.C. 4.1.1.15) in a culture medium, wherein said microorganism is a Corynebacterium ,wherein glutamate is converted to GABA in said recombinant microorganism, and wherein said heterologous glutamate decarboxylase is encoded by a nucleic acid composing: a) the nucleotide sequence from position 472 to 1200 of SEQ ID NO: 1 or from position 193 to 1605 of SEQ ID NO: 1; b) a nucleotide sequence encoding a glutamate decarboxylase comprising the amino acid sequence of SEQ ID NO: 2; or c) a nucleotide sequence encoding a glutamate decarboxylase comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 2, wherein said glutamate decarboxylase comprises the amino acid sequence from Thr94 to Leu336 of SEQ ID NO: 2 or an amino acid sequence having at least 98% sequence identity to the amino acid sequence from Thr94 to Leu336 of SEQ ID NO: 2.