Patent ID: 7393443

Claim:
A method of identifying the presence of kinetically stable proteins in a protein library comprising assaying the library for proteins that are resistant to denaturation by SDS and correlating said resistance to denaturation by SDS with the presence of kinetically stable proteins, wherein the assaying step comprises the steps of: a) providing at least one pair of substantially identical protein samples from the library; b) adding SDS to each sample of the pair of protein samples; c) boiling only one of the protein samples of the pair of protein samples; d) conducting SDS-polyacrylamide gel electrophoresis on the pair of protein samples; e) comparing the migration on the gel of proteins contained in the boiled protein sample to the migration on the gel of proteins contained in the unheated protein sample; and f) correlating proteins migrating slower in the unheated protein sample with resistance to denaturation by SDS.