Patent ID: 8449881

Claim:
A purified monoclonal antibody, or antigen-binding fragment thereof, comprising a heavy chain immunoglobulin variable domain and a light chain immunoglobulin variable domain that binds to α-enolase I, wherein the light chain immunoglobulin variable domain comprises the amino acid sequence of: (i) SGGSGSYG (SEQ ID NO: 1), SGGSSSYGYG (SEQ ID NO: 2), SGSSGSYG (SEQ ID NO: 3), SGGSSSYGYS (SEQ ID NO: 4) or SGSSGYGYG (SEQ ID NO: 5) in CDR1, (ii) ANTNRPS (SEQ ID NO: 6), NDNQRPS (SEQ ID NO: 7), RDDKRPS (SEQ ID NO: 8), SNNQRPS (SEQ ID NO: 9) or SNDKRPS (SEQ ID NO: 10) in CDR2, and (iii) GGYDSSAGI (SEQ ID NO: 11), GSGDSSTGM (SEQ ID NO: 12), GSGESSTNNGI (SEQ ID NO: 13), GSMDSSNSGV (SEQ ID NO: 14) or GGYDSSASYVGI (SEQ ID NO: 15) in CDR3; and wherein the heavy chain immunoglobulin variable domain comprises the amino acid sequence of: (i) SFNMF (SEQ ID NO: 16), SHDMG (SEQ ID NO: 17), DYCVQ (SEQ ID NO: 18), SFYMF (SEQ ID NO: 19) or SYAMH (SEQ ID NO: 20) in CDR1, (ii) GINNAGSTTNHGAAVKG (SEQ ID NO: 21), GIENAAGIGTFYGAAVKG (SEQ ID NO: 22), AISNTGRYTGYGSAVKG (SEQ ID NO: 23), GISGDGRYTGYGAAVDG (SEQ ID NO: 24) or GISRDGGSSTRYYGAAVKG (SEQ ID NO: 25) in CDR2, and (iii) SPGGIDGIDG (SEQ ID NO: 26), GADTGGWPAANIDA (SEQ ID NO: 27), DGCAGCCGSYYIDG (SEQ ID NO: 28) ESGSGCCNGDNIDA (SEQ ID NO: 29) or DSDNGGYYCDDIDA (SEQ ID NO: 30) in CDR3.