Patent ID: 8629412

Claim:
A fluorescence intensity correction method comprising: receiving, by a plurality of light detectors having different light-receiving wavelength bands, fluorescence emitted from fluorescent pigments that are excited by irradiating light on microparticles that are multiply labeled by a plurality of fluorescent pigments, collecting detected values from each light detector, and obtaining a fluorescence spectrum of each fluorescent pigment as one spectrum group; separating the obtained spectrum group into a plurality of small spectrum groups; comparing the separated small spectrum groups with a fluorescence wavelength distribution of each fluorescent pigment, which is obtained in advance, and specifying the small spectrum groups as the fluorescence spectrum of a fluorescent pigment of the plurality of fluorescent pigments; comparing a differential spectrum between small spectrum groups that are not specified as the fluorescence spectrum of any fluorescent pigment and one or more specified small spectrum groups with the fluorescence wavelength distribution of unspecified fluorescent pigments, which are obtained in advance, and specifying the differential spectrum as the fluorescence spectrum of a fluorescent pigment of the plurality of fluorescent pigments; and calculating the intensity of fluorescence emitted from each fluorescent pigment using a fluorescence wavelength distribution of the small spectrum group or the differential spectrum with regard to fluorescent pigments in which the small spectrum group or the differential spectrum is specified and using the fluorescence wavelength distribution, which is obtained in advance, with regard to fluorescent pigments in which the small spectrum group or the differential spectrum is not specified.