Patent ID: 7790040

Claim:
A method for isocratically purifying a target molecule having endogenous selective affinity for a ligand from a mixture of molecules in a feedstock using a simulated moving bed chromatography system, the system comprising a plurality of chromatography columns substantially filled with ligand-bound resin, inlets for feedstock and eluent, and outlets for extract and raffinate, the method comprising: selecting a concentration of an affinity neutralizing agent sufficient to prevent static binding of the target molecule to the ligand-bound resin while selectively retarding passage of the target molecule through the ligand-bound resin in a mobile phase in contrast to molecules in the mixture not having such affinity; adjusting the concentration of the affinity neutralizing agent in the feedstock and eluent to the selected concentration; and processing the feedstock through the simulated moving bed chromatography system to recover a purified target molecule from the extract outlet.