Patent ID: 8680023

Claim:
A method for forming an array of receptors for M13 bacteriophages, comprising: patterning an array of structures on a first substrate to form a template on a surface of the first substrate; applying a protein receptor material to a face of a second substrate; contacting the face of the second substrate with the template to remove a portion of the receptor material from the second substrate, thereby forming a subtractive pattern of an array of the protein receptor material on the second substrate, wherein the second substrate is a featureless elastomer having a deformable planar surface; contacting a target substrate with the subtractive pattern of the array of the protein receptor material on the second substrate to transfer the subtractive pattern of the array of the protein receptor material onto the target substrate, wherein the subtractive pattern of the array of the protein receptor material disposed on the target substrate comprises discrete locations of the protein receptor material on the target substrate each discrete location of the protein receptor material having a plurality of receptor sites; and contacting the target substrate with a solution comprising M13 bacteriophages at a concentration of 107 to 109 plaque forming units per millimeter and selectively binding specific M13 bacteriophages to the array of the protein receptor material at the discrete locations on the target substrate, wherein the discrete locations are dimensioned such that a majority of the discrete locations are occupied by a single one of the specific M13 bacteriophages at the discrete location, wherein an average lateral dimension of each array of protein receptor material is from 120 nanometers to 200 nanometers and the array of protein receptor material has an average pitch of 5 micrometers to 700 nanometers.