Patent ID: 8232080

Claim:
A method of converting biomass to sugars comprising contacting the biomass with a CBH1 polypeptide having at least 96.4% sequence identity to SEQ ID NO:2, comprising one or more amino acid substitutions selected from the group consisting of Q17L, G22D, T41I, N49S, S57N, A68T, A77D, S92T, N103I, A112E, S113(T/N/D), E193V, S196T, M213I, P227(L/T/A), T246(C/A), D249K, Y252(A/Q), T255P, D257E, D259W, S278P, S279N, K286M, L288F, E295K, T296P, S297T, N301(R/K), E325K, T332(K/Y/H), F338Y, S342Y, F352L, T356L, Y371C, T380G, Y381D, V393G, S398T, V403D, S411F, G430F, G440R, T462I, T484S, Q487L, and P491L, or with a composition comprising the CBH1 polypeptide.