Patent ID: 7879543

Claim:
A method for detecting in a nucleic acid sample the presence or absence of at least two variant nucleotides associated with thrombosis, the method comprising the steps of: a) amplifying from the sample regions of DNA that include at least two selected nucleotide positions for which variants are known to be associated with thrombosis, to form amplified DNA products; b) hybridizing at least two tagged allele specific extension primers to a complementary target sequence in the amplified DNA products, wherein each tagged allele specific extension primer has a 3′-end hybridizing portion capable of hybridizing to the corresponding amplified DNA and a 5′-end tag portion complementary to a corresponding anti-tag sequence, the terminal nucleotide of the 3′ end hybridizing portion being either complementary to a suspected variant nucleotide or to the corresponding wild type nucleotide, the nucleotide sequences of the at least two tagged allele specific extension primers consisting of sequences selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, and SEQ ID NO: 12; c) extending the at least two tagged allele specific extension primers, using labelled nucleotides, if the terminal nucleotide of each 3′ end hybridizing portion is a perfect match to the corresponding amplified DNA product; and d) hybridizing the at least two tagged allele specific extension primers to their corresponding anti-tag sequences and detecting the presence of labelled extension products.