Patent ID: 7067059

Claim:
A method of generating a separation medium including mixed mode cation-exchanger ligands coupled to a base matrix, which method comprises the steps of (a) providing at least one scaffold, said scaffold including a functional group (F) and having a cyclic core structure; (b) derivatizing each of the scaffold(s) with a reagent, which includes a reactive group (Z) coupled to a residue (R), by reacting the functional group of said scaffold with the reactive group of said reagent, while retaining the cyclic core structure of the scaffold; (c) opening up of the cyclic structure of the resulting derivative; and (d) reacting the opened product so obtained with a base matrix including a reactive group; wherein said scaffold presents at least two functionalities, one of which is a sulphur-comprising group for coupling to the reactive group of the base matrix and one of which is a group that can be transformed into an ionic group, said functionalities being present on the cyclic structure in adjacent positions, and wherein in step (c), opening up is provided by breaking the bond between two functionalities.