Patent ID: 8664260

Claim:
In a method of detecting cells or particles by flow cytometry, wherein said cells or particles are labelled with a detectable luminescent compound, the improvement comprising utilizing a bacteriochlorin as the luminescent compound, wherein said bacteriochlorin is a compound of Formula I: wherein: M is a metal or is absent; X is NH; R 1 and R 2 are each independently selected from the group consisting of H, alkyl, aryl, alkoxy, halo, mercapto, cyano, hydroxyl, nitro, acyl, alkylthio, alkylamino, acyloxy, and —C(O)NR a R b where R a and R b are each independently selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl, aryl, and linking groups; R 3 and R 4 are each independently selected from the group consisting of alkyl, cycloalkyl, aryl, arylalkyl, and linking groups; R 5 is selected from the group consisting of H, alkyl, alkenyl, alkynyl, cycloalkyl, aryl arylalkyl, arylalkenyl, arylalkynyl, alkoxy, halo, cyano, nitro, acyl, alkylthio, alkylamino, arylalkylamino, disubstituted amino, acylamino, acyloxy, and —C(O)NR a R b where R a and R b are each independently selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl, aryl, and linking groups; and R 6 , R 7 , and R 8 are each independently selected from the group consisting of H, alkyl, alkenyl, alkynyl, cycloalkyl, aryl, arylalkyl, arylalkenyl, arylalkynyl, halo, cyano, nitro, acyl, alkoxy, alkylthio, alkylamino, arylalkylamino, disubstituted amino, acylamino, acyloxy, —C(O)NR a R b where R a and R b are each independently selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl and aryl, —C(O)OR c where R c is selected from the group consisting of alkyl, cycloalkyl, alkenyl, alkynyl and aryl, C(O)OH, and linking groups.