Patent ID: 8921112

Claim:
A method for inserting a sequence into an endogenous inositol polyphosphate 2-kinase (IPP2K) gene in a maize cell, the method comprising: expressing, in the maize cell, a pair of zinc finger nucleases that bind to and cleave the plant cellular chromatin in a region of interest, wherein the region of interest is in an IPP2K gene and further wherein at least one zinc finger nuclease comprises a zinc finger protein having 4, 5 or 6 zinc finger domains designated and ordered F1 to F4, F1 to F5 or F1 to F6, each zinc finger domain comprising a recognition helix region and further wherein the zinc finger protein is selected from the group consisting of a protein comprising the recognition helix regions of the sequence and order as shown in a single row of Table 8; and introducing the sequence into the maize cell such that the sequence is inserted into at least two alleles of the same IPP2K gene in the region of interest.