Patent ID: 8268600

Claim:
A process for the preparation of ethanol by Kluyveromyces species IIPE453 MTCC 5314, comprising the steps: (a) culturing the strain Kluyveromyces species IIPE453 MTCC 5314 in salt medium 1 (SM1) wherein the said SM1 comprising 0.15 g/l di-sodium hydrogen ortho-phosphate, 0.15 g/l potassium di-hydrogen ortho-phosphate, 2.0 g/l ammonium sulphate, 1.0 g/l yeast extract and 10 g/l glucose with pH in the range of 5.0 to 6.5 at a temperature in the range of 37° C. to 55° C. for a period of 24 to 30 hours under stirring to obtain cell biomass; (b) inoculating the cell biomass as obtained in step (a) in fresh salt medium 2 (SM2) wherein the said SM2 comprises 0.15 g/l di-sodium hydrogen ortho phosphate, 0.15 g/l potassium di-hydrogen ortho phosphate, 1.0 g/l ammonium sulphate, 1.0 g/l yeast extract supplemented with 50 to 100 g/l of a fermentable substrate selected from the group consisting of monosaccharide and disaccharide sugars, bagasse, hydrolysate of lignocellulosic biomass, starch based biomass, either singly and in any combination thereof and with pH in the range of 3.5 to 6.5 at a temperature in the range of 45° C. to 60° C. for a period of 24 to 48 hours under stirring to obtain fermentation broth containing ethanol; or (c) immobilizing the cell biomass as obtained in step (a) onto bagasse chips and inoculating the immobilized biomass in fresh salt medium 2 (SM2) wherein the said SM2 comprises 0.15 g/l di-sodium hydrogen ortho phosphate, 0.15 g/l potassium di-hydrogen ortho phosphate, 1.0 g/l ammonium sulphate, 1.0 g/l yeast extract supplemented with 50 to 100 g/l of a fermentable substrate with pH in the range of 3.5 to 6.5 at a temperature in the range of 45° C. to 60° C. for a period of 40 to 90 hours under stirring to obtain fermentation broth containing ethanol; and (d) separating the ethanol from the fermentation broth.