Patent ID: 8198052

Claim:
A method for detecting a Beta-actin mRNA in a sample by performing rapid loop-mediated isothermal polymerase chain reaction in intraoperative diagnosis, comprising: synthesizing cDNA from the mRNA of Beta-actin in the sample by using a primer set comprising a first primer, a second primer, a third primer, a fourth primer, a fifth loop primer and a sixth loop primer, an enzyme with reverse transcriptase activity, and dNTP substrates, amplifying the cDNA by using the primers, a DNA polymerase for strand displacement synthesis, and dNTP substrates, and detecting the mRNA of the Beta-actin by detecting amplified cDNA, wherein the first primer comprises the polynucleotide sequence set forth in SEQ ID NO: 37, the second primer comprises the polynucleotide sequence set forth in SEQ ID NO: 45, the third primer comprises the polynucleotide sequence set forth in SEQ ID NO: 10, the fourth primer comprises the polynucleotide sequence set forth in SEQ ID NO: 31, the fifth primer comprises the polynucleotide sequence set forth in SEQ. ID NO: 33, and the sixth primer comprises the polynucleotide sequence set forth in SEQ ID NO: 34.