Patent ID: 7052866

Claim:
A method for evaluating the efficacy of an antifungal agent, comprising: a) administering the antifungal agent to a living mammalian body infected with a fungus, b) taking a fragment from a site of the administration of the agent thereafter, c) quantitating the fungus in the fragment by the steps of: 1) cultivating the fragment from the administration site infected with the fungus and administered with the antifungal agent, in a medium containing a phospholipid, a nonionic surfactant, or both of the phospholipid and the nonionic surfactant, and 2) detecting the grown fungus, and comparing an amount of fungus obtained when the fragment is cultivated in a medium containing a phospholipid, a nonionic surfactant, or both the phospholipid and the nonionic surfactant with, an amount obtained when the fragment is cultivated in a medium without a phospholipid or a nonionic surfactant, whereby the influence of the antifungal agent remaining in the fragment is eliminated by the cultivation in the medium containing the phospholipid and/or non-ionic surfactant.