Patent ID: 8710199

Claim:
A sensor domain for enzyme-assisted molecular delivery, the sensor domain comprising a protection segment comprising an RNA portion, and a targeting domain binding portion presented for covalent attachment to a targeting domain; an activation segment comprising a DNA portion complementary to the RNA portion of the protection segment; a displacement segment complementary to the protection segment, the displacement segment complementarily binding the protection segment; and a toehold segment complementary to a signal polynucleotide, wherein the protection segment, displacement segment, and activation segment are configured so that upon complementary binding of the signal polynucleotide to the toehold segment, the displacement segment is displaced from the protection segment and the DNA portion of the activation segment complementarily binds the RNA portion of the protection segment to provide an RNAase H binding site presented for binding; wherein the targeting domain binding portion and the RNA portion of the protection segment are configured to allow release of the targeting domain upon cleavage of the RNAase H binding site; wherein each of the displacement segment, the protection segment, the activation segment and the toehold segment have a 5′ terminus and a 3′ terminus, and wherein the 5′ of the activation segment is adjacent to the 3′ terminus of the protection segment, the 3′ terminus of the activation segment is adjacent to the 5′ terminus of the displacement segment, the 3′ terminus of the displacement segment is adjacent to the 5′ terminus of the toehold segment.