Patent ID: 7553935

Claim:
A method of increasing clot formation in a mammal, said method comprising administering to said mammal an amount of a mutant Factor VII (FVII) or mutant Factor VIIa (FVIIa) polypeptide effective to increase clot formation in said mammal, wherein said mutant FVII or mutant FVIIa polypeptide comprises a modified γ-carboxyglutamic (GLA) domain that enhances membrane binding affinity of said mutant FVII or mutant FVIIa polypeptide relative to a wild type human Factor VII (hFVII) or wild type human Factor VIIa (hFVIIa) polypeptide, wherein said modified GLA domain comprises an amino acid sequence that differs from the amino acid sequence of the GLA domain of hFVII or hFVIIa polypeptide shown in SEQ ID NO: 3 in 1 to 5 amino acid substitutions, wherein the proline residue at amino acid position 10 of SEQ ID NO:3 is substituted with a glutamine, aspartic acid or glutamic acid residue in the modified GLA domain.