Patent ID: 8309742

Claim:
A process for preparing a compound of the formula II comprising the steps of: reacting an α,β-unsaturated ketone compound of the formula I with donor substrates using a PigD protein as a catalyst to effect 1,4 additions of said substrates onto said compound of formula I, wherein said donor substrates comprise straight-chain, saturated 2-oxoalkanoates or 2-oxocarboxylic acids with 1-10 carbon atoms and comprising the formula R 4 C(O)COO − or R 4 C(O)COOH, respectively, in which R 1 is a straight-chain or branched C 1 -C 10 -alkyl radical or an aromatic comprising a substituted or unsubstituted phenyl group, R 2 is a hydrogen, C 1 -C 10 -alkyl radical or an aromatic comprising a substituted or unsubstituted phenyl group; R 3 is a straight-chain or branched C 1 -C 10 -alkyl radical, a heterocycle comprising a substituted or unsubstituted phenyl group or an aromatic comprising a substituted or unsubstituted phenyl group, where the C 1 -C 10 -alkyl radical may in turn have a heterocycle comprising a 5- or 6-membered heterocyclic ring or an aromatic comprising a substituted or unsubstituted phenyl group, and where the heterocycle or the aromatic may be substituted by methoxy groups, halogens or hydroxyl groups, and R 4 is a straight-chain or branched C 1 -C 10 -alkyl radical.