Patent ID: 6999175

Claim:
A method of determining interaction of at least one species with a plurality of individual zones of a sensing surface, which method comprises: contacting the sensing surface with a fluid sample containing at least one surface interacting species, irradiating the surface with light so that the light is internally or externally reflected at the surface, imaging reflected light on a photo-detector, each individual sensing surface zone corresponding to a respective area of the detector, repeatedly varying the incident angle at the sensing surface and/or the wavelength of the light over an angular and/or wavelength range, measuring the intensities of light imaged on different areas of the detector, at at least a number of incident angles and/or wavelengths to create a series of images of the sensing surface, simultaneously measuring the momentary incident angle and/or wavelength of the incident light to correlate each image to a specific incident angle and/or wavelength to obtain at least fifty correlated images per second, and determining from the relationship between image intensity data and angular and/or wavelength data, interaction of the species with the individual sensing surface zones.