Patent ID: 7550570

Claim:
A labeled nucleotide according to structural formula (VII): wherein: B is selected from the group consisting of a 7-deazapurine nucleotide base attached to the illustrated furan at its N9 position, a purine nucleotide base attached to the furan of structural formula (VII) at its N9 position and a pyrimidine nucleotide base attached to the furan of structural formula (VII) at its N1 position; D is a 4,7-dichlororhodamine dye according to structural formula (VI): wherein: R 7 , R 8 , R 9 , R 10 , R 12 , R 13 , R 14 , R 15 , R 16 and R 18 are each, independently of one another, selected from the group consisting of hydrogen, fluorine, chlorine, methyl, ethyl, lower alkyl, lower alkene, lower alkyne, cycloalkyl, phenyl, aryl, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy and combinations thereof, or, alternatively, R 7 and R 8 or R 13 and R 14 may be taken together for form an oxo, sulfoxo, imminium or alkyliminium group; R 11 and R 17 are each, independently of one another, selected from the group consisting of hydrogen, lower alkyl, alkyl sulfonate, alkyl carboxylate, lower alkene, lower alkyne, cycloalkyl, phenyl, aryl and combinations thereof; and X 1 , X 2 and X 3 are each, independently of one another, selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, amine, amide, carboxylate, sulfonate and hydroxymethyl, with the proviso that one of R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 14 , R 15 , R 16 , R 17 , R 18 , X 1 , X 2 or X 3 comprises a linkage linking D to B; W 1 and W 2 are each, independently of one another, selected from the group consisting of hydrogen and OH; W 3 is selected from the group consisting of OH, OPO 3 , OP 2 O 6 , OP 3 O 9 , and analogs thereof.