Patent ID: 8084587

Claim:
A method for the stabilization of a gamma-carboxyglutamic (GLA)-residue containing serine protease, said method comprising subjecting a solution comprising the protease to one or more stabilization steps, wherein (i) the GLA-residue containing serine protease is a human Factor VII polypeptide variant wherein the ratio between the activity of the Factor VII polypeptide variant and the activity of wild type human Factor VIIa (SEQ ID NO: 1) is at least about 1.25 when tested in an in vitro hydrolysis assay; (ii) wherein said solution comprising the factor VII polypeptide is subjected to the steps of adding calcium to obtain a molar ratio of free calcium ions and factor one polypeptide (Ca2+: FVII polypeptide) lower than 0.5 and adjusting the pH to which the protease is subjected to a value between 4.5 and 6.9 or has a value between 8.8 and 9.8.