Patent ID: 8679737

Claim:
A microfluid device for producing diffusively built gradients, comprising a bottom plate and a cover plate, wherein the cover plate includes a plurality of recesses and is connected with the bottom plate in a liquid-tight manner so that the recesses form at least two reservoirs immediately adjacent to an observation chamber, wherein the observation chamber provides fluid communication between the reservoirs at a respective at least two first junctures with the reservoirs on respective at least two first sides of the observation chamber, the cover plate includes a first inlet through which at least one said reservoir can be filled, the cross-sectional area of the observation chamber at each of the first juncture with the reservoirs is at least 5 times smaller than the maximum cross-sectional area of the reservoir, the microfluid device additionally comprises at least one channel with at least a second inlet at the cover plate, wherein the at least one channel is in fluid communication with the observation chamber at a respective at least one second juncture with the observation chamber at a respective at least one second side of the observation chamber different than the first sides, and the observation chamber includes at least one cell adhesion surface.