Patent ID: 7807367

Claim:
A kit for determining cAMP content or an adenylate cyclase activity in a biological sample which comprises: (1) a vial for Cleaning Reaction comprising effective amounts of apyrase, alkaline phosphatase and adenosine deaminase without 5′-nucleotidase to remove non-cyclic adenine nucleotides consisting of endogenous ATP, ADP and AMP, and endogenous glucose-6-phosphate in a biological sample; (2) a vial for Converting Reaction comprising an effective amount of phosphodiesterase to enzymatically convert cAMP in a biological sample into AMP; and (3) a vial for Detecting Reaction comprising glycogen, inorganic phosphoric acid and glycogen phosphorylase to convert glycogen into glucose-1-phosphoric acid; phosphoglucomutase to convert glucose-1-phosphate into glucose-6-phosphate; and glucose-6-phosphate dehydrogenase and NADP + to convert glucose-6-phosphate into 6-phosphogluconolactone and NADPH.