Patent ID: 6867022

Claim:
A vector system for selectively packaging a replication defective adenovirus nucleic acid sequence in an adenovirus capsid based on adenovirus serotype, the vector system comprising: (a) a first adenovirus nucleic acid sequence comprising: (i) 5′ and 3′ adenovirus inverted terminal repeats (ITRs); (ii) a first adenovirus serotype-specific cis-acting packaging sequence; and (iii) a heterologous nucleic acid operably linked to a transcriptional control sequence; wherein the first adenovirus nucleic acid sequence comprises a defective or modified adenovirus E1 gene, E2A gene, E2B gene, E3 gene, E4 gene, E4 promoter, penton gene, fiber gene, hexon gene, or a combination thereof, and wherein the first adenovirus nucleic acid fails to encode a 52/55 kDa trans-acting protein specific for the first cis-acting packing sequence; (b) a second adenovirus nucleic acid sequence comprising: (i) 5′ and 3′ adenovirus ITRs; (ii) a second adenovirus serotype-specific cis-acting packaging sequence; and wherein the second adenovirus nucleic acid sequence complements the defective or modified adenovirus E1 gene, E2A gene, E2B gene, E3 gene, E4 gene, E4 promoter, penton gene, fiber gene, hexon gene, or a combination thereof of the first adenovirus nucleic acid sequence, and wherein the second adenovirus nucleic acid fails to encode a 52/55 kDa trans acting protein specific for the second cis-acting packing sequence; (c) an adenovirus replication competent host cell comprising a nucleic acid sequence encoding an adenovirus 52/55 kDa trans-acting protein that supports packaging of the first adenovirus nucleic acid sequence and fails to support packaging of the second adenovirus nucleic acid sequence, wherein the replication defective adenovirus comprises the first adenovirus nucleic acid sequence.