Patent ID: 7238523

Claim:
A plasmid containing a cDNA sequence which encodes a protein designated p53as, said p53as being sequentially the same as wildtype p53 up to the final 50 carboxy terminal amino acids of p53, said p53as being different than p53 within the final 50 carboxy terminal amino acids of p53 so as to lack a negative regulatory domain of p53 for p53 sequence specific DNA binding found within the last 50 amino acids at the p53 carboxy terminus, which negative regulatory domain must be activated in p53 for p53 to have active DNA binding, said p53as and activated p53 binding to the same p53 DNA binding sequence AGGCATGCCT/AGGCATGCCT SEQ ID NO: 5 and said p53as being different than p53 within the final 50 carboxy terminal amino acids of p53 so as to provide an epitope within said p53as which gives rise to an antibody which is reactive with the p53as but not with p53.