Patent ID: 7754461

Claim:
A method for obtaining a human butyrylcholinesterase preparation from a Cohn Fraction IV-4 paste of human origin wherein said human butyrylcholinesterase preparation comprises 41.4 to 56.1% of the butyrylcholinesterase activity present in the Cohn Fraction IV-4 paste and has a purity of about 99% or more and a specific activity of about 700 U/mg, which comprises obtaining a Cohn Fraction IV-5 supernatant from the Cohn Fraction IV-4 paste; subjecting the Cohn Fraction IV-5 supernatant to procainamide affinity chromatography only once by loading the human butyrylcholinesterase in the Cohn Fraction IV-5 supernatant onto an affinity chromatography column, washing the human butyrylcholinesterase on the affinity chromatography column with a volume of a wash solution that is 54 to 84 times the volume of chromatographic media used for procainamide affinity chromatography, and obtaining an eluent, subjecting the eluent to anion exchange chromatography only once, and obtaining the human butyrylcholinesterase preparation.