Patent ID: 8153401

Claim:
A method of performing a polymerase chain reaction (PCR) comprising; providing a crude sample comprising deoxyribonucleic acid; incubating the crude sample with a direct buffer to form a nucleic acid-containing solution; and performing a polymerase chain reaction (PCR) on said nucleic acid-containing solution, wherein the direct buffer comprises at least 5 PCR primer pairs, Tris-HCl at 10-16 mM, KCl at 50-75 mM, dNTPs at 200-400 uM each dNTP, BSA at 800-960 ug/ml, AmpliTaq Gold polymerase at 2U-8U, MgCl2 at 1.6-2.2 mM, and single-stranded binding protein (ssb) of at least 20 ng/ul.