Patent ID: 7247457

Claim:
A method of detecting an enterovirus (EV) RNA sequence in a sample, comprising: contacting the sample with one or more nucleic acid cDNA primers comprising a sequence as set forth in any one of SEQ ID NOs: 1-4, that hybridize to an EV VP1 encoding sequence; reverse transcribing EV cDNA from the EV RNA sequence; amplifying at least a portion of the EV cDNA using a first nucleic acid primer pair, wherein the first forward primer comprises a sequence as set forth in SEQ ID NO: 5 and hybridizes to an EV VP3 encoding sequence, and the first reverse primer comprises a sequence as set forth in SEQ ID NO: 6 and hybridizes to an EV VP1 encoding sequence, thereby generating an EV amplicon; amplifying at least a portion of the EV amplicon using a second nucleic acid primer pair, wherein the second forward primer comprises a sequence as set forth in SEQ ID NO: 11 or SEQ ID NO: 13, and the second reverse primer comprises a sequence as set forth in SEQ ID NO: 12 or SEQ ID NO: 14, wherein both the second forward and reverse primers hybridize to an EV VP1 encoding sequence; and determining whether an amplified EV amplicon is present, thereby detecting an EV nucleic acid sequence in the sample.