Patent ID: 7632392

Claim:
A sensor of pyrophosphate comprising an insulative substrate, and an electrode system that is formed on the insulative substrate and has at least a measurement electrode and a counter electrode, wherein: pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, a nucleotide, an electronic mediator, and a buffer component are carried on the substrate; the pyrophosphatase is separated from the buffer component; the glyceraldehyde-3-phosphate is separated from the buffer component; the glyceraldehyde-3-phosphate dehydrogenase is separated from every one of the buffer component, the pyrophosphatase, and the glyceraldehyde-3-phosphate dehydrogenase; and the nucleotide is oxidized nicotineamide adenine dinucleotide, oxidized nicotineamide adenine dinucleotide phosphate, or a combination thereof.