Patent ID: 8163511

Claim:
A method for the identification of a compound that is an inhibitor of the kinase activity of mutant leucine-rich repeat kinase 2 (LRRK2), comprising the steps of a) providing a protein preparation containing wild type or mutant LRRK2, b) contacting the protein preparation with indol ligand 91 immobilized on a solid support under conditions allowing the formation of a complex of indol ligand 91 with LRRK2 of a), c) incubating the indol ligand 91-LRRK2 complex with a given compound, and d) determining whether the compound separates LRRK2 of a) from the immobilized indol ligand 91; wherein step d) comprises the detection of separated or bound LRRK2 of a) or the determination of the amount of separated or bound LRRK2 of a); wherein separation of LRRK2 of a) from indol ligand 91 by said compound indicates that said compound interacts with LRRK2 of a); and e) determining whether said compound that interacts with LRRK2 of a) is an inhibitor of the kinase activity of mutant LRRK2, wherein said mutant LRRK2 has the same or different amino acid sequence as the LRRK2 of a), wherein each mutant LRRK2 of a) and e) is independently selected from the group consisting of: G2019S, R1441C, Y1699C, I2020T, R793M, Q930R, R1067Q, S1096C, I1122V, S1228T, I1371V, R1441G, R1441H, R1514Q, M1869T, R1941H, I2012T, T2356I, and G2385R.