Patent ID: 8155409

Claim:
A computer implemented method for processing of microscopic images to detect objects of interest comprising: subjecting the microscopic image to a bandpass filtering to obtain a filtered image, wherein the bandpass filtering is such as to suppress the noise and any objects which are larger than a predetermined size; and processing the filtered image at a plurality of progressively decreasing threshold levels, said processing comprising: at each threshold level detecting the objects of interest using an object labelling algorithm; and removing the objects detected at a given threshold level from the working image before proceeding to the next threshold level, wherein the upper threshold level of the threshold range over which the processing of the filtered image is applied is set at a half the maximum intensity in the filtered image; and the lower threshold level of the threshold range over which the processing of the filtered image is applied is estimated by multiplying the intensity corresponding to the maximum of the intensity histogram of the filtered image with a user defined factor.