Patent ID: 7625725

Claim:
A method of enhancing a nucleic acid polymerase reaction comprising: (a) forming a nucleic acid polymerase reaction composition comprising: (i) a nucleic acid (ii) at least one nucleic acid polymerase selected from a Pfu DNA polymerase, an exo − Pfu DNA polymerase, a Pwo DNA polymerase, a Vent DNA polymerase, a Deep Vent DNA polymerase, a JDF3 DNA polymerase, and an ES4 DNA polymerase, and (iii) a P45 protein, wherein the P45 protein is in monomeric, dimeric, or multimeric form, and wherein the P45 protein is produced from a cell containing a DNA construct comprising a sequence encoding polymerase enhancing factor protein P45 operably linked to an expression vector, and wherein the P45 protein comprises the sequence of SEQ ID NO:72, and (b) incubating the nucleic acid polymerase reaction composition under conditions allowing a nucleic acid polymerase reaction, wherein the P45 protein increases rate, fidelity and/or yield of the nucleic acid polymerase reaction.