Patent ID: 7741028

Claim:
A method of identifying the presence or absence of genetic markers in the human cystic fibrosis transmembrane conductance regulator (CFTR) gene of a subject comprising: providing a DNA sample from said subject; contacting said DNA sample with at least four primer sets; generating an extension product from each of said at least four primer sets comprising a region of DNA that includes the location of a genetic marker; grouping said extension products according to the following grouping assignments, wherein at least one group includes at least four pooled extension products: Group 1 comprising extension products generated from primer sets SEQ ID NOs 358-359, SEQ ID NOs 364-365, and SEQ ID NOs 186-187; Group 2 comprising extension products generated from primer sets SEQ ID NOs 235-236, SEQ ID NOs 305-306, SEQ ID NOs 226-227; Group 3 comprising extension products generated from primer sets SEQ ID NOs 262-263, SEQ ID NOs 330-331, SEQ ID NOs 319-320, SEQ ID NOs 221-222, and SEQ ID NOs 325-326; Group 4 comprising extension products generated from primer sets SEQ ID NOs 267-268, SEQ ID NOs 203-204, SEQ ID NOs 230-231, SEQ ID NOs 191-192, SEQ ID NOs 284-285, SEQ ID NOs 219-220, and SEQ ID NOs 205-206; Group 5 comprising extension products generated from primer sets SEQ ID NOs 376-377, SEQ ID NOs 378-379, SEQ ID NOs 280-281, SEQ ID NOs 237-238, SEQ ID NOs 335-336, SEQ ID NOs 312-313, SEQ ID NOs 255-256, SEQ ID NOs 330-331, SEQ ID NOs 277-278, SEQ ID NOs 235-236, SEQ ID NOs 189-190, SEQ ID NOs 282-283, and SEQ ID NOs 248-249; Group 6 comprising extension products generated from primer sets SEQ ID NOs 269-270 SEQ ID NOs 257-258, SEQ ID NOs 198-199, and SEQ ID NOs 337-338; Group 7 comprising extension products generated from primer sets SEQ ID NOs 271-272, SEQ ID NOs 328-329, SEQ ID NOs 314-315, SEQ ID NOs 296-297, SEQ ID NOs 242-243, SEQ ID NOs 367-368, SEQ ID NOs 182-183, and SEQ ID NOs 360-361; Group 8 comprising extension products generated from primer sets SEQ ID NOs 371-372, SEQ ID NOs 341-342, SEQ ID NOs 355-356, SEQ ID NOs 339-340, and SEQ ID NOs 298-299; Group 9 comprising extension products generated from primer sets SEQ ID NOs 273-274 and SEQ ID NOs 323-324; Group 10 comprising extension products generated from primer sets SEQ ID NOs 369-370, SEQ ID NOs 321-322, SEQ ID NOs 228-229, and SEQ ID NOs 212-213; Group 11 comprising extension products generated from primer sets SEQ ID NOs 210-211, SEQ ID NOs 383-384, and SEQ ID NOs 381-382; Group 12 comprising extension products generated from primer sets SEQ ID NOs 275-276; Group 13 comprising extension products generated from primer sets SEQ ID NOs 175-176 and SEQ ID NOs 177-178; separating said extension products on the basis of melting behavior; and identifying the presence or absence of said genetic markers in said subject by analyzing the melting behavior of said extension products.