Patent ID: 7972856

Claim:
An in vitro method of producing a vertebrate cell having a genetic modification at a preselected genomic target locus, the method comprising: (i) transducing into the vertebrate cell an effective amount of a recombinant parvovirus particle genome that comprises: a) a targeting construct which comprises a DNA sequence which is substantially identical to the genomic target locus except for the modification being introduced, wherein the modification being introduced is flanked by regions substantially identical to the genomic target locus, said regions being at least 100 nucleotides in length; and b) at least one parvoviral inverted terminal repeat (ITR) flanking the targeting construct; and (ii) determining if the genomic target locus has the introduced genetic modification, wherein the targeting construct facilitates homologous pairing between the targeting construct and the genomic target locus resulting in the genetic modification being introduced into the genomic target locus.