Patent ID: 7608423

Claim:
A kit for producing a protein that comprises at least one unnatural amino acid in a eukaryotic cell, the kit comprising: a container containing a polynucleotide sequence encoding an orthogonal tRNA (O-tRNA), and a polynucleotide sequence encoding an orthogonal aminoacyl tRNA synthetase (O-RS), wherein: the O-RS preferentially aminoacylates the O-tRNA with the unnatural amino acid, and wherein the O-RS comprises the polypeptide sequence selected from the group consisting of: SEQ ID NO: 36-47 and 86; the cell produces the protein in the absence of the unnatural amino acid with a yield that is less than 10% of the yield in the presence of the unnatural amino acid; and the O-tRNA specifically recognizes a selector codon in a recombinant nucleic acid of interest in the cell.