Patent ID: 7141411

Claim:
A simple, safe, and efficient process for the complete bio-decaffeination of caffeine-containing solutions using strain Pseudomonas alcaligenes CFR 1708, said process comprising steps of: a. culturing the strain in caffeine agar plates, b. transferring actively growing culture into nutrient broth containing 0.1–0.5 g/L caffeine, c. incubating culture of step (b) at about 28–30° C. for about 20–24 hrs under agitation of between about 50–200 rpm to obtain pre-inoculum, d. transferring the pre-inoculum to nutrient broth comprising about 0.5–2 g/L caffeine, e. incubating the pre-inoculum at about 28–30° C. for about 20–24 hours under agitation between about 50–200 rpm to accumulate biomass, f. harvesting the biomass by centrifugation for about 10–20 mm at about 10,000–16,000 g at temperature ranging between about 0–4° C. to obtain pellet, g. inducing the pellet for caffeine degradation by aseptically transferring the pellet into sterilized caffeine liquid medium of pH about 5–9, h. incubating the induced pellet at about 25–35° C. under agitation between about 50–200 rotations per minute for time duration of about 36–60 hours, and i. decaffeinating caffeine-containing solution by incubating with the pellet of step (h).