Patent ID: 8765418

Claim:
A method for detecting the presence or absence of a HPV target nucleic acid in a sample, wherein said HPV target nucleic acid is from one or more strains in a set of strains of HPV, comprising: a) providing: i) a sample suspected of containing a HPV target nucleic acid; ii) a composition comprising a mixture of different oligonucleotide probe sets, wherein: A) each of said oligonucleotide probe sets comprises a first oligonucleotide and a second oligonucleotide that in combination form an invasive cleavage structure with target nucleic acid from at least two different strains of HPV in said set of strains of HPV; B) each of said oligonucleotide probe sets forms an invasive cleavage structure with target nucleic acid from fewer than all of the different strains in said set of strains of HPV; and C) at least two of said probe sets in said mixture are configured to form an invasive cleavage structure with target nucleic acid from each different strain of HPV in said set of strains of HPV; and iii) a 5′ nuclease; b) exposing said sample to said composition under reaction conditions in which, if HPV target nucleic acid from one or more strains in said set of strains is present, at least two of said oligonucleotide probe sets hybridize to said HPV target nucleic acid to form at least two invasive cleavage structures, and in which said at least two invasive cleavage structures are cleaved with said 5′ nuclease; and c) detecting cleavage of said invasive cleavage structures, wherein detection of cleavage of said invasive cleavage structures is indicative of the presence of HPV target nucleic acid from at least one strain of said set of strains of HPV in said sample.