Patent ID: 8445288

Claim:
A method for analyzing a terminal monosaccharide on a glycosylated substrate, comprising (i) detaching said monosaccharide from said glycosylated substrate by contacting with an exoglucosidase; (ii) allowing said detached monosaccharide to covalently bind to a capture group on a solid support; (iii) incubating said covalently bound monosaccharide with a detection agent of formula X: TAG-R-Boronate (Formula X) wherein TAG=a tag moiety capable of being detected R=organic moiety Boronate=a boronic acid moiety or ester thereof, said boronate being attached to a carbon atom comprised in said R group; (iv) allowing the detection agent to bind to the monosaccharide; and (v) detecting, directly or indirectly, the detection agent that is bound to the monosaccharide.