Patent ID: 7615380

Claim:
A method of identifying compounds useful in modulating a biological activity of a mammalian Kappa Recognition Component (KRC) polypeptide comprising: a) providing an indicator composition comprising a KRC polypeptide encoded by a nucleic acid molecule which hybridizes to the complement of a nucleic acid molecule set forth in SEQ ID NO: 1 over the full length of the isolated nucleic acid molecule in 6× SSC at 45° C. followed by one or more washes in 0.2× SSC, 0.1% SDS at 65° C., wherein said polypeptide comprises a TNF Receptor Associated Factor (TRAF) interacting domain comprising amino acid residues 204-1055 of SEQ ID NO:2 and two zinc finger-acidic domain structure (ZAS) DNA binding domains, and wherein said polypeptide modulates immune cell proliferation, TNF-α induced apoptosis, and signaling via an NFKB or JNK signaling pathway; b) providing a TRAF or c-Jun polypeptide; c) contacting the indicator composition with each member of a library of test compounds; d) measuring the ability of the KRC polypeptide to interact with the c-Jun or TRAF polypeptide; e) determining the ability of the test compound to modulate the interaction of the KRC polypeptide with the c-Jun polypeptide or of the KRC polypeptide with the TRAF polypeptide; and f) selecting from the library of test compounds a compound of interest that modulates the ability of the KRC polypeptide to interact with the c-Jun or TRAF polypeptide as compared to an appropriate control, to thereby identify a compound that modulates a biological activity of mammalian KRC polypeptide.