Patent ID: 7396685

Claim:
A method for obtaining assignments of chemical shift values of 1 H, 13 C and 15 N of a protein molecule comprising: providing a 15 N/ 13 C-labeled protein sample; and conducting four reduced dimensionality (RD) nuclear magnetic resonance (NMR) experiments on the protein sample, wherein the chemical shift values of 1 H and 13 C which are encoded in peak pairs of an NMR spectrum are detected in a phase sensitive manner and (1) a first experiment is selected from the group consisting of a RD three-dimensional (3D) H α/β C α/β (CO)NHN NMR experiment, a RD 3D HA , CA ,(CO),N,HN NMR experiment, and a RD 3D H , C ,(C-TOCSY-CO),N,HN NMR experiment for obtaining sequential correlations of chemical shift values; (2) a second experiment is selected from the group consisting of a RD 3D HNN CAHA NMR experiment, a RD 3D H α/β , C α/β ,N,HN NMR experiment, and a RD 3D HNN< CO , CA > NMR experiment for obtaining intraresidue correlations of chemical shift values; (3) a third experiment is a RD 3D H , C ,C,H-COSY NMR experiment for obtaining assignments of sidechain chemical shift values; and (4) a fourth experiment is a RD two-dimensional (2D) HB , CB ,(CG,CD),HD NMR experiment for obtaining assignments of aromatic sidechain chemical shift values.