Patent ID: 7575858

Claim:
A method of quantifying a protein analyte, comprising: (1) staining at least one protein analyte spot and a series of protein standard spots of known quantity bound to a protein-binding membrane with a staining reagent comprising reactive brown 10, or a salt thereof (2) incubating said protein analyte spot and said protein standard spots bound to the protein-binding membrane with the staining reagent for a time interval sufficient to allow reaction of the protein spot and said protein standard spots with the staining reagent; (3) destaining the staining reagent from the protein-binding membrane; (4) generating image quantification data for the known protein standard spots and for the protein analyte spot; (5) constructing a standard calibration curve using the known concentrations of the protein standard and the corresponding image quantification data; and (6) calculating a concentration for the protein analyte.