Patent ID: 7399836

Claim:
A method of manufacturing a carrier having immobilized antibodies, comprising: providing antiligands on and surrounding a surface of a carrier, the carrier having a substantially spherical shape and at least the surface of the carrier is formed of a calcium phosphate based compound; treating a protein containing calcium ion to remove or reduce the calcium ion to thereby obtain a treated protein, the protein having low interaction with antigens or antibodies; forming a blocking layer comprising the treated protein on at least a portion of the surface of the carrier where the antiligands are not provided subsequent to the providing of the antiligands on and surrounding the surface of the carrier; and immobilizing antibodies to the surface of the carrier, each of the antibodies having a constant region on which a ligand is provided, and each of the antibodies is immobilized to the surface of the carrier by bonding between the ligands and antiligands, and the blocking layer effectively preventing antibodies from being directly absorbed to the surface without bonding between the ligand and the antiligands.