Patent ID: 7262283

Claim:
A method for the sequential production of partial proteomes from a complete proteome of a cell preparation comprising: a) providing a sample containing a cell preparation b) extracting the cytosolic proteins and the membrane/organelle proteins from the sample provided in step a), leaving a cell nucleus preparation, and c) extracting the proteins from the cell nucleus interior from the preparation obtained in step b) by treating with an extraction buffer having a pH of between 6.5 and 8 which comprises: in total from 0.1 to 7 percent by weight of one or more nonionic detergents in total from 0.05 to 3 percent by weight of one or more cholic acid derivatives, and one or more salts of alkali metal and/or ammonium in a total concentration of between 75 and 500 mmol/l, wherein detergent-resistant proteins of the cytoskeleton and of the nuclear matrix are not extracted to a significant extent together with the proteins from the cell nucleus interior, but instead remain in the extraction residue.