Patent ID: 8367353

Claim:
A method for discriminating between dysplastic cervical and non-dysplastic cervical tissue, said method comprising: (a) obtaining a cervical tissue sample from a patient; (b) determining whether any one single cell in the sample co-expresses a first and a second protein marker molecule, wherein the first protein marker molecule is p16 INK4a and the second protein marker molecule is selected from the group consisting of Ki67, Ki-S5, Ki-S2, MCM2, MCM3, MCM4, MCM5, MCM6, MCM7; and (c) determining the tissue is dysplastic if there is at least one cell in the sample expressing the first protein marker molecule and simultaneously expressing the second protein marker molecule or determining the tissue is non-dysplastic if no cells co-expressing the first and second protein marker molecules are detected in the sample, thereby discriminating between dysplastic and non-dysplastic cervical tissue.