Patent ID: 7794954

Claim:
A non radioisotopic method of determining canine T4AA in a patient sample comprising: (a) measuring the amount of Free T4 in a patient sample, wherein the sample is from a canine species, the Free T4 being attached as Free T4/T4AA complex when T4AA is present in the patient sample together with Free T4 unattached to T4AA; (b) treating the patient sample with a non-radioisotopic precipitating or binding agent to precipitate or bind Free T4/T4AA complex thereby forming a complex of precipitated or bound Free T4/T4AA complex; (c) suspending the non-radioisotopic precipitate or bound complex, and at least one of: (i) measuring the amount of Free T4/T4AA complex in suspended non-radioisotopic precipitate or bound complex using a non-radioisotopic label, and (ii) subtracting the measurement of i) from a) wherein the result of ii) is directly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample; or (iii) measuring Free T4 in supernatant or surrounding fluid of the non-radioisotopic precipitate or bound complex using a non-radioisotopic label; (iv) subtracting the measurement of iii) from a) wherein the result of iv) is indirectly proportional to the amount of Free T4/T4AA complex thereby determining the amount of T4AA in the patient sample; effecting detection with a sensitivity in a range between 1 picogram per mL and up to about 1 femtogram per mL.