Patent ID: 8637638

Claim:
A composition comprising two different proteins produced by a process comprising the steps of: (a) isolating a gene encoding a full length sequence of a human V H region isolated from a B cell clone of a human patient having either a refractory low grade lymphoma or follicular B cell lymphoma; (b) linking said gene encoding said entire V H region and a gene encoding at least a portion of an immunoglobulin constant region in an expression vector to form a gene encoding the first of said different proteins; (c) isolating a gone encoding a full length sequence of a human V L region isolated from a B cell clone of said patient having said refractory low grade lymphoma or follicular B cell lymphoma; (d) linking said gene encoding said entire V L region and a gene encoding at least a portion of an immunoglobulin constant region into said expression vector to form a gene encoding the second of said different proteins; (e) introducing said expression vector into insect cells, wherein said expression vector contains two separate expression cassettes each having a baculovirus promoter and a heterologous secretory signal sequence; and (f) allowing the expression of said two different proteins having the same V H and V L amino acid sequences in said patient, wherein said first protein and/or said second protein is further conjugated to a carrier protein, wherein said composition further comprises a cytokine or chemokine, and wherein said composition elicits an active immune response.