Patent ID: 8338088

Claim:
A method of determining one or more characteristics of the glycosylation pattern of a recombinant non-cell surface glycoprotein of interest produced by a cell, the method comprising: a) providing a cell that produces the recombinant non-cell surface glycoprotein of interest, which cell has at least one cell-surface glycan attached to the surface of the cell, so that the cell has a cell surface glycosylation pattern; b) liberating at least one cell surface glycan from the cell; c) determining at least one characteristic of the cell surface glycosylation pattern selected from the group consisting of degree of glycosylation site occupancy, identity of at least one linked glycan, relative amounts of linked glycans, complete or partial composition of linked glycans, and combinations thereof; and d) based on the at least one determined characteristic, establishing one or more characteristics of the glycosylation pattern of the recombinant non-cell surface glycoprotein of interest, wherein the determined characteristic correlates with the one or more characteristics of the glycosylation pattern of the non-cell surface glycoprotein of interest.