Patent ID: 8357836

Claim:
An Agrobacterium -mediated method for producing a transformed maize or rice, which comprises: (i) a coculture step for culturing an Agrobacterium -inoculated immature embryo with a coculture medium; and (ii) a regeneration step for culturing the immature embryo obtained in (i) with a regeneration medium, either without callus growth culture or after callus growth culture, to thereby regenerate a whole transformed maize or rice, wherein 1) said method comprises transformation enhancement, wherein the transformation enhancement is selected from the group consisting of: thermal treatment; centrifugation; thermal treatment and centrifugation; pressurization; addition of silver nitrate and/or copper sulfate to the coculture medium; inoculation with Agrobacterium in the presence of a powder; addition of carbenicillin to the medium in the callus growth and/or regeneration step following the coculture step; addition of N6 inorganic salts to the callus growth medium; and addition of cysteine to the coculture medium, and wherein 2) said method does not comprise any selection step based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.