Patent ID: 8304192

Claim:
A method of assessing allele frequency of a first allele and a second allele of a target nucleic acid in a sample, comprising: co-amplifying said target nucleic acid and a known amount of a competitive template for said target nucleic acid; co-amplifying a reference nucleic acid and a known amount of a competitive template for said reference nucleic acid; obtaining a first relation, said first relation comparing amplified product of said target nucleic acid corresponding to said first allele to amplified product of said competitive template for said target nucleic acid; obtaining a second relation, said second relation comparing amplified product of said target nucleic acid corresponding to said second allele to amplified product of said competitive template for said target nucleic acid; obtaining a third relation, said third relation comparing amplified product of said reference nucleic acid to amplified product of said competitive template for said reference nucleic acid; comparing one of said first, second or third relations to both of said other relations, thereby assessing allele frequency of said first and said second alleles in said sample; and detecting false positives of said first allele by the steps of: co-amplifying a known amount of positive control for said second allele and a known amount of said competitive template for said target nucleic acid; and obtaining a fourth relation, said fourth relation comparing amplified product of said positive control for said second allele that is detectable by a detection moiety specific for said first allele to amplified product of said competitive template for said target nucleic acid, wherein obtaining said first, second and/or third relation comprises a use of an oligonucleotide array and wherein said amplified products of said target nucleic acid corresponding to said first allele, of said target nucleic acid corresponding to said second allele and of said competitive template for said target nucleic acid are immobilized by a first common capture moiety.