Patent ID: 6846645

Claim:
A method for simultaneously assaying the activities of multiple kinases in a cell-signaling pathway, the method comprising the steps of: providing in a reaction mixture a set of kinase substrates under conditions effective to convert a kinase substrate in the set to a corresponding product in the presence of the kinase for that substrate, where (i) each kinase substrate and the product of that kinase substrate have different separation characteristics from each other and from the other kinase substrates in the set and their corresponding products and (ii) each kinase substrate and its corresponding product have a detection moiety capable of producing a detectable signal; separating the kinase substrates and products in said reactions in a single separation medium; detecting, for each separated product, a separation characteristic effective to identify that product and a signal related to the amount of the product; and determining, from the detected separation characteristic and signal detected for each product, the amount of each kinase substrate converted to the corresponding product in said reaction mixture.