Patent ID: 7981654

Claim:
A cellulase fusion protein comprising: first amino acid sequence, which is the 20 K cellulase of Melanocarpus albomyces of SEQ ID NO:2, or the 20K cellulase of Melanocarpus albomyces of SEQ ID NO:2 having at least one modification selected from the group consisting of: deletion of Ala at position 207, deletion of Val at position 208, substitution of Phe209Trp, and insertion of Pro after position 206, or a fragment thereof having cellulase activity, and a second amino acid sequence, which is the linker and cellulose binding domain (CBD) of Trichoderma reesei cellobiohydrolase I of SEQ ID NO:4, or the linker and cellulose biding domain (CBD) of Trichoderma reesei cellobiohydrolase I of SEQ ID NO: 4 having at least one modification selected from the group consisting of: substitution of tyrosine at position 492 (position 31 of the CBD), 493 (position 32 of the CBD) of the mature T. reesei CBHI with an aliphatic or aromatic amino acid, and deletions of amino acids 434 to 444 or 434 to 460 of the mature T. reesei CBHI sequence, or a fragment thereof having CBD activity, and a junction region having the following general formula: 1 A- 2 B- 3 Ile- 4 D- 5 Ser- 6 F (SEQ ID NO: 85) wherein 1 A is Gly or Val; 2 B is Pro, Gln, or Glu; 4 D is Gly or Pro; and 6 F is Ser or is absent between said first amino acid sequence and said second amino acid sequence, whereby a stable fusion protein is obtained.