Patent ID: 6897020

Claim:
A method of elucidating a protein expression profile of a test cell line or group of cells, the method comprising: (A) randomly introducing into the genome of a cell or group of cells a promoterless polynucleotide construct, the construct comprising in a 5′ to 3′ orientation: i) a splice acceptor consensus sequence; ii) the complementary sequence of a type IIS restriction enzyme recognition sequence; iii) an oligonucleotide sequence encoding assayable marker peptide; and iv) a polyadenylation sequence; (B) wherein said promoterless polynucleotide construct when introduced into an actively expressed gene results in the generation of a truncated cellular protein fused at its C-terminal truncated end to the marker peptide; and i) identifying those cells expressing said marker peptide fused to said truncated cellular protein; ii) determining the identity of the truncated proteins to which the marker peptide is fused in each group of cells, thereby elucidating a protein expression profile of a test cell line of group of cells.