Patent ID: 7598093

Claim:
A method of detecting a secreted cell product, comprising: a) providing a reaction chamber comprising: cell culture and detection substrate upon which a cell that produces and secretes the cell product can adhere and a complex formed by the cell product with a detection particle is visualized as a spot on said substrate; said detection substrate having capture reagent thereon that binds to said cell product to form a product-capture reagent complex; b) adding a sample of cells to said reaction chamber and permitting at least one of said cells to adhere to said substrate; c) stimulating said cells to produce said product, wherein said product binds to said capture reagent to form a product-capture reagent complex at a location near said cell on said substrate and then washing away said cells to expose said location; d) adding a detection reagent to said reaction chamber, wherein said detection reagent binds to said product producing a detection reagent-cell product-capture reagent complex (DRC); e) adding a detection particle to said chamber, wherein said detection particle binds to said detection reagent via streptavidin/biotin binding producing a detection particle-detection reagent-cell product-capture reagent complex (DRCC); and f) visualizing said detection particle on said DRCC as a spot on said substrate.