Patent ID: 8440457

Claim:
A method for purification of a cell population from a sample containing multiple cell populations, comprising the following sequential steps: a) contacting a sample with a cell-type-specific binding reagent linked to a polyvalent matrix by mixing said reagent with the sample so that a complex of said binding reagent and a cell population of interest forms; b) immobilizing the complex obtained from step a); c) removing the material that is not immobilized; d) releasing the immobilized complex; e) contacting the sample obtained from step d) with an additional amount of the specific binding reagent linked to a polyvalent matrix by mixing the additional amount of binding reagent with said sample; f) immobilizing the complex obtained from step e); g) removing the material that is not immobilized; h) releasing the immobilized complex; and i) recovering a cell population bound to the binding reagent.