Patent ID: 8765375

Claim:
A method of sequencing one or more target polynucleotides, the method comprising the steps of: fragmenting a predetermined coverage amount of the one or more target polynucleotides to form a population containing overlapping fragments; forming a number of separate mixtures of origin from the population wherein at least sixty percent of the separate mixtures contain only non-overlapping fragments, and optionally subfragmenting at least some of the fragments in said mixtures, thereby forming subfragments, wherein the respective mixture of origin of the fragments or subfragments is determinable; then obtaining nucleic acid sequence reads for at least a portion of a plurality of the fragments or subfragments; and obtaining complete or partial nucleotide sequences of the one or more target polynucleotides by assembling sequence reads for the fragments or subfragments, wherein the assembling comprises determining the mixtures of origin of the fragments or subfragments from which the sequence reads were obtained.