Patent ID: 8273571

Claim:
A method for producing primate gynogenetic pluripotent cells comprising: (a) providing a primate oocyte in metaphase II; (b) activating said primate oocyte using activation conditions selected from the group consisting of: contacting the oocyte with a calcium ionophore, contacting the oocyte with a protein synthesis inhibitor, contacting the oocyte with phosphorylation inhibitor, and any combination thereof, to produce an activated oocyte, (c) culturing said activated oocyte to produce a gynogenetic blastocyst having an inner cell mass; (d) isolating said inner cell mass or isolated cells therefrom and transferring said inner cell mass or isolated cells therefrom into an in vitro culture that inhibits differentiation of said inner cell mass or isolated cells therefrom; and (e) culturing said inner cell mass or isolated cells therefrom or cells derived therefrom during inner cell mass proliferation in a culture that maintains the inner cell mass cells or cells therefrom in the undifferentiated state thereby producing a primate gynogenetic pluripotent stem cell.