Patent ID: 6942969

Claim:
A method for determining susceptibility of a HCMV (human cytomegalovirus) for an anti-viral drug comprising: (a) culturing a host cell in the presence of the anti-viral drug, wherein the host cell has introduced thereto a resistance test vector comprising: (1) a patient-derived segment that comprises an HCMV gene, and (2) an indicator gene, wherein the expression of the indicator gene is dependent upon the patient-derived segment; (b) measuring the expression of the indicator gene in the host cell; and (c) comparing the expression of the indicator gene measured in (b) to the expression of indicator gene measured in a corresponding host cell, cultured in the absence of the anti-viral drug, having introduced thereto a corresponding resistance test vector comprising: (1) a patient-derived segment that comprises a HCMV gene, and (2) an indicator gene, wherein the expression of the indicator gene is dependent upon the patient-derived segment, wherein greater expression of the indicator gene in the absence of the anti-viral drug relative to that measured in the presence of the anti-viral drug indicates susceptibility of the HCMV for the anti-viral drug.