Patent ID: 8828727

Claim:
A method of displaying a candidate polypeptide on the surface of a mammalian display cell, comprising the steps of: a) contacting a packaging cell with a viral display vector comprising a nucleic acid sequence encoding a fusion protein under conditions such that said packaging cell generates one or more viral particles comprising said nucleic acid sequence, wherein said fusion protein comprises: i) a leader peptide, wherein said leader peptide directs the fusion protein to the surface of said mammalian cell; ii) a candidate polypeptide; iii) at least a portion of an immunoglobulin molecule, wherein said portion comprises the hinge region, the CH2 region or the CH3 region of an IgG molecule or a combination thereof, and wherein said portion is not the candidate polypeptide; iv) a mammalian transmembrane moiety; v) an envelope incorporation signal; and b) contacting said viral particles with a mammalian display cell, wherein said mammalian display cell expresses said fusion protein and said candidate polypeptide is displayed on the surface of said mammalian display cell, and wherein said packaging cell further comprises an envelope vector comprising an env gene operably linked to a promoter and a polyadenylation sequence wherein said env gene encodes a functional envelope protein and a helper vector comprising one or more viral structural genes.