Patent ID: 8653237

Claim:
An isolated peptide comprising P0-P1-P2-P3-P4-P5-P6-P7-P8-P9-P10, wherein P1-P2-P3-P4-P5-P6-P7-P8-P9 comprises a substitution in at least one position of the PRAME 425-433 sequence SLLQHLIGL (SEQ ID NO: 71), wherein the at least one position is at amino acid position P1, P2, P6, or P9, wherein the peptide has an affinity for a class I MHC binding cleft that is similar to or greater than the affinity of SLLQHLIGL (SEQ ID NO: 71) for said class I MHC binding cleft, wherein P0 is X, XX, or XXX, wherein X specifies any amino acid or no amino acid, and wherein P10 is X, XX, or XXX, wherein X specifies any amino acid or no amino acid, wherein P1 is selected from the group consisting of S, K, F, Y, T, Orn, and Hse, wherein P2 is selected from the group consisting of L, V, M, I, Nva, Nle, and Abu, wherein P6 is selected from the group consisting of L, Nva, Nle, or Abu, and wherein P9 is selected from the group consisting of L, V, I, A, Nle, Nva, Abu, and L-NH 2 .