Patent ID: 7713741

Claim:
A method for calibrating a photoluminescence measurement system, in particular a fluorescence measurement system, the method comprising: (a) measuring fluorescence spectra (J i (λ)) of a plurality of fluorescence standards (i) with the photoluminescence measurement system to be calibrated, wherein the fluorescence standards (i) are selected, so that their combined spectrally corrected and normalized fluorescence spectra (I i (λ)) cover a predefined spectral range in such a way, that the fluorescence bands of the spectrally corrected and normalized fluorescence spectra (I i (λ)) have at least a minimum predefined intensity at mutual crossover wavelengths (λ i/i+1 ), wherein the spectrally corrected and normalized fluorescence spectra (I i (λ)) is sequentially arranged; (b) calculating combination factors (α i ) through statistical averaging of consecutive partial correction functions (F i (λ)), wherein the partial correction functions (F i (λ)) are formed by forming the quotient of the measured fluorescence spectra (J i (λ)) and the corresponding spectrally corrected and normalized fluorescence spectra (I i (λ)) of the fluorescence standards (i), and wherein the statistical averaging takes place over a predefined, limited overlap region (λ i/i+1 ±Δλ OL ) about the mutual crossover wavelengths (λ i/i+1 ); and (c) determining a total correction function (F(λ)), for the predefined spectral range by combination of the partial correction functions (F i (λ)), which are weighted according to the combination factors (α i ).