Patent ID: 8399857

Claim:
A method for resolution-enhanced luminescence microscopy comprising exciting a sample to the emission of luminescence radiation through irradiation by excitation radiation, and acquiring an image of the luminescing sample, wherein a first partial volume of said sample is irradiated by a first laser radiation field of the excitation radiation, and a second partial volume of said sample is irradiated by a second laser radiation field of the excitation radiation, such that both partial volumes emit said luminescence radiation, wherein said first partial volume of said sample and said second partial volume of said sample overlap one another partially but not completely, resulting in the sample luminescing over both, the first and second partial volumes, wherein either only the first laser radiation field is modulated with a first frequency, or the first laser radiation field is modulated with a first frequency and the second laser radiation field is modulated with a second frequency being different to the first radiation frequency, thus making only the luminescence radiation emitted from the first partial volumes being modulated with the first frequency, and wherein the luminescence radiation of the sample is detected with modulation filtering so that the luminescence radiation from the second partial volume of the sample is suppressed and the luminescence radiation from the first partial volume is extracted from regions of the first partial volume which do not overlap with the second partial volume resulting in the resolution enhancement.