Patent ID: 7598356

Claim:
A method for purifying a protein of the cystine-knot superfamily comprising: (a) applying a sample containing a protein of the cystine-knot superfamily to a dye affinity chromatography matrix comprising a dye ligand, wherein the sample is selected from the group consisting of a tissue, a bodily fluid and a culture medium; (b) removing contaminants from the dye affinity chromatography matrix; (c) eluting the cystine-knot protein from the dye affinity chromatography matrix; (d) applying the eluted cystine-knot protein to a reversed-phase chromatography matrix; (e) removing contaminants from the reversed-phase chromatography matrix; and (f) eluting the cystine-knot protein from the reversed-phase chromatography matrix thereby purifying the protein of the cystine-knot superfamily.