Patent ID: 8822226

Claim:
A method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography, wherein the elution process comprises: analysing filtrates extracted from a tobacco sample in accordance with the following elution process and conditions; Concentration Time of KOH (min) (ρ/mmol · L −1 ) Curve Description 0.000 1 5 Start of analysis 8.000 1 5 Analysis of weak retention components 28.000 30 5 Analysis of medium retention components 35.000 50 5 Analysis of strong retention components 40.000 50 5 Purge system 40.001 1 5 Balance system 50.000 1 5 End of analysis conditions of said ion chromatograghy being: anion exchange column: IonPacAS11-HC (2 mm×50 mm) and IonPacAG11-HC (2 mm×250 mm); column temperature: 30° C.; flow rate: 0.30 ml/min; injection volume: 25 μL; suppressor current: 50 mA; and wherein the method further comprises steps for pre-treating the tobacco sample before analysis, the steps for pre-treating the tobacco sample consisting of: (1) determining the percentage of moisture in the tobacco sample; (2) pre-treating the tobacco sample by extracting the tobacco sample ultrasonically by extraultra-purified water at a ratio of 1 g tobacco sample: 10 mL water and then filtering the sample to collect the filtrates; and (3) diluting the filtrates tenfold and then filtering the filtrates.