Patent ID: 7993842

Claim:
A method for amplifying a target polynucleotide, comprising: a) providing a plurality of oligonucleotide primer pairs, wherein each oligonucleotide primer pair is located at a discrete position on a microarray, and wherein each oligonucleotide primer in each oligonucleotide primer pair comprises at least one cleavable linkage; b) hybridizing a target polynucleotide to at least one primer in at least one oligonucleotide primer pair on the microarray, thereby producing a microarray comprising at least one primer pair comprising at least one primer hybridized to a target polynucleotide; c) embedding the microarray of step b) into a capturing support; d) separating the at least one primer oligonucleotide pair comprising at least one primer hybridized to a target polynucleotide from the microarray by cleaving the at least one cleavable linkage in each primer, thereby releasing the at least one oligonucleotide primer pair comprising at least one primer hybridized to a target polynucleotide from the microarray, wherein the at least one oligonucleotide primer pair comprising at least one primer hybridized to a target polynucleotide is captured in the capturing support; and e) amplifying the target polynucleotide under conditions in which the at least one oligonucleotide primer pair comprising at least one primer hybridized to the target polynucleotide amplifies the target polynucleotide by polymerase chain reaction, whereby an amplified target polynucleotide is produced; thereby amplifying a target polynucleotide.