Patent ID: 8594946

Claim:
A method for genotyping comprising: a) pooling n individual samples to form a pool of multiple samples wherein the individual samples in the pool are present in a molar ratio of x 0 :x 1 :x 2 :x (n−1) , wherein x is equal to a positive value other than 1; b) quantitatively measuring a signal in the pool, wherein the intensity of the signal relates to the number of alleles present in the pool, and allocating the result of the measurement to one out of y* ((p+1) 0 +(p+1) 1 +(p+1) 2 +(p+1) (n−1) )values, wherein y is the number of potential different alleles, p is the ploidy level, and wherein the lowest value is 0%, being equal to the minimum possible signal strength, the highest is 100%, being equal to the maximum possible signal strength, and the intermediate values are arranged according to equal intervals of (1/y* ((p+1) 0 +(p+1) 1 +(p+1) 2 +(p+1) (n−1) ))* 100%; and, c) determining the genotype of each individual sample in the pool from the value allocated in b), taking into account the molar ratio at which each individual sample contributed to the pool of multiple samples.