Patent ID: 8641986

Claim:
An assay cartridge comprising: a cartridge body having a first side and one or more first side cover layers mated to said first side such that said cartridge body and said first side cover layers define one or more first side fluidic networks therebetween, wherein said assay cartridge includes a sample chamber having a sample introduction port with a sealable closure and a sample chamber vent port connected to said sample chamber; a first waste chamber and a first waste chamber vent port connected to said waste chamber; a first reagent chamber, a first reagent chamber vent port connected to said first reagent chamber, and a first reagent chamber conduit connecting said first reagent chamber with a first sample conduit, a second reagent chamber, a second reagent chamber vent port connected to said second reagent chamber, and a second reagent chamber conduit connecting said second reagent chamber with a second sample conduit, and a first detection chamber connected to said sample chamber by said first sample conduit and to said first waste chamber by a first waste conduit, wherein said sample and waste chamber vent ports are configured to equilibrate fluid in said sample and waste chambers, respectively, with the atmosphere.