Patent ID: 8333932

Claim:
A substrate comprising: a plurality of distinct fiducial marks disposed on a surface of the substrate; a plurality of distinct areas on the surface of the substrate on which a probe material is configured to be immobilized, each of the areas being separated from the fiducial marks and from each other; and a probe immobilization compound disposed on at least one of the areas on the surface of the substrate on which the probe material is configured to be immobilized, wherein each fiducial mark has a structure which reflects irradiated light at a greater intensity than an intensity of reflected irradiated light from areas on the substrate not corresponding to the fiducial mark; wherein each fiducial mark comprises at least two pillars and is separated from the areas on the surface of the substrate on which a probe material is configured to be immobilized; and wherein a distance between the at least two pillars is from about 0.001 μm to about 10000 μm.