Patent ID: 8357833

Claim:
A method of producing a transgenic corn plant with increased asparagine comprising: a) introducing into a corn plant a heterologous DNA construct comprising a promoter molecule functional in a corn cell operably linked to a DNA molecule encoding an asparagine synthetase polypeptide; b) growing the plant to maturity; and c) harvesting seed from the plant, wherein the DNA molecule encoding an asparagine synthetase polypeptide is selected from the group consisting of: i) a nucleic acid sequence comprising the sequence of SEQ ID NO:50; ii) a nucleic acid sequence with at least 95% sequence identity to the sequence of SEQ ID NO:50 over its full length, wherein the nucleic acid sequence encodes a polypeptide with asparagine synthetase activity; iii) a nucleic acid sequence that encodes the polypeptide sequence of SEQ ID NO:51; and iv) a nucleic acid sequence that encodes a polypeptide sequence with at least 98% sequence identity to the sequence of SEQ ID NO:51 over its full length, wherein the nucleic acid sequence encodes a polypeptide comprising asparagine synthetase activity; wherein said seed produces a transgenic corn plant with increased asparagine when compared to a non-transgenic corn plant.