Patent ID: 8257828

Claim:
A method for forming a coated article for use in cell culture, comprising: conjugating a polymerization initiator to a surface of the article to form an initiator-conjugated surface; contacting the initiator-conjugated surface with monomers; activating the initiator to initiate polymerization and graft the polymer to the surface via the initiator, or a remnant thereof; wherein the polymer is a swellable (meth)acrylate polymer having an equilibrium water content of between 5% and 70%, wherein the polymer is formed from a mixture of monomers comprising a carboxyl group-containing (meth)acrylate monomer, a cross-linking (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer, wherein the weight percentage of the polymer derived from the hydrophilic monomer is between 60% and 90%, the weight percentage of the polymer derived from the carboxyl group-containing monomer is between 10% and 40%, and the weight percentage of the polymer derived from the cross-linking monomer is between 1% and 10%; and, conjugating a cell adhesive polypeptide having an RGD sequence to the polymer.