Patent ID: 8658365

Claim:
A method of sequencing a nucleic acid, comprising: providing a complex comprising a Phi-29 DNA polymerase enzyme, a template nucleic acid, and primer sequence complementary to at least a portion of the template nucleic acid; contacting the complex with a reaction mixture that comprises a mixture of divalent metal ions comprising at least one catalytic metal ion and at least one non-catalytic metal ion, wherein the catalytic metal is selected from Mg 2+ , Mn 2+ and mixtures thereof, and the non-catalytic metal is selected from Ca 2+ , Sr 2+ , or Ba 2+ and mixtures thereof, and one or more types of nucleotides or nucleotide analogs wherein a ratio of catalytic metal to non-catalytic metal in the reaction mixture is from about 10:1 to about 1:5; detecting incorporation of one or more of the nucleotide into the complex; and using the detected incorporation to determine the sequence of the template nucleic acid.