Patent ID: 8722377

Claim:
A coloration method comprising the steps of: (a) mixing peptide and tyrosinase; (b) incubating the mixture; (c) adjusting the pH of the mixture from 8 to 11; (d) a color reaction for coloration of bright green to blue colors with the mixing of tyrosinase and peptide; and (e) cryopreserving the incubated mixture wherein the tyrosinase is obtained from Pholiota nameko and is encoded by a polynucleotide selected from the group consisting of: (1) a polynucleotide consisting of a base sequence set forth in SEQ ID NO: 1 or 3; (2) a polynucleotide encoding the amino acid sequence set forth in SEQ ID NO: 2 or 4; (3) a polynucleotide hybridizing to a fully complementary strand of any one of the polynucleotides (1) to (2) under highly stringent conditions; wherein the highly stringent condition related to hybridization and washing is 0.0015M sodium chloride, 0.0015M sodium citrate, 65-68° C., 50% formamide, and 42° C., and wherein the amino acid at the N-terminus of the peptide is glycine.