Patent ID: 8691973

Claim:
A method of producing a porous chitosan scaffold, the method comprising: providing an aqueous acidic solution comprising: chitosan, and a solvent which does not dissolve the chitosan, wherein the solvent which does not dissolve the chitosan is selected from the group consisting of a C3-C8 aliphatic alcohol having one hydroxy group, ethylene glycol monoethylether, ethylene glycol monobutylether, dioxane, tetrahydrofuran, dimethylcarbonate, acetone and acetonitrile, and the amount of the solvent which does not dissolve the chitosan is in the range of 0.001 to 30% (v/v), based on the volume of the solution; and freeze-drying the aqueous acidic solution to form a porous chitosan scaffold, wherein the solvent which does not dissolve the chitosan is present during freeze-drying.