Patent ID: 8835632

Claim:
A dye of Formula (II); wherein: m is an integer from 1 to 4; X 2 is halogen; R 1 , R 2 , R 3 , R 6 , and R 7 are each independently selected from the group consisting of H, alkyl, substituted alkyl, heteroalkyl, substituted heteroalkyl, cycloalkyl, substituted cycloalkyl, cycloheteroalkyl, substituted cycloheteroalkyl, aryl, substituted aryl, aralkyl, hydroxyl, alkoxyl, hydroxyalkyl, hydroxycycloalkyl, alkoxycycloalkyl, aminoalkyl, acyloxyl, alkylaminoalkyl, alkoxycarbonyl, alkyleneaminoalkyl, alkylenesulfate, —(CH 2 ) p —SO 3 , —(CH 2 ) p —N + (CH 3 ) 2 —(CH 2 ) p NCS, and —(CH 2 ) p —NH—CO—CH 2 —X 2 ; wherein X 2 is halogen; R 5 is selected from the group consisting of alkyl, substituted alkyl, cycloalkyl, substituted cycloalkyl, cycloheteroalkyl, substituted cycloheteroalkyl, aryl, substituted aryl, aralkyl, hydroxyl, alkoxyl, hydroxyalkyl, hydroxycycloalkyl, alkoxycycloalkyl, aminoalkyl, acyloxyl, alkylaminoalkyl, alkoxycarbonyl, alkyleneaminoalkyl, alkylenesulfate, —(CH 2 ) p —SO 3 , —(CH 2 ) p —N + CH 3 ) 2 —(CH 2 ) p —NCS, and —(CH 2 ) p —NH—CO—CH 2 —X 2 ; wherein X 2 is halogen; R 8 is selected from the group consisting of H, alkyl, substituted alkyl, hydroxyl, amino, nitro, alkoxyl, and hydroxyalkyl; Y 1 is H; Y 2 is selected from the group consisting of H, —NH 2 , —SO 3 − , —(CH 2 ) t OH, —(CH 2 ) t NH 3 + X 2 − ; CH 3 CONH—, X 2 CH 2 CONH—, HO(CH 2 ) 2 —S—CH 2 CONH—, SuOCOCH 2 OCH 2 CON(CH 3 )—, —CH 2 —NH—C(═O)—O—(CH 3 ) 3 , —(CH 2 ) t —NH 2 + —(CH 2 ) s —SO 3 − , —(CH 2 ) t —NH—C(═O)—(CH 2 ) s —X 2 , a succinimidyl ester, a hydroxysuccinimide, a maleimide, a haloacetyl, a pyridyl disulfide, a hydrazine, an ethyldiethylamino propylcarbodiimide, or a protecting group, including t-butoxycarbonyl and carbobenzoxy; and wherein each p, r, s and t is independently an integer from 1 to 8; and Su is succinimidyl ester; and salts thereof.