Patent ID: 7919472

Claim:
A method of reducing expression of a target RNA in an animal, in need of reducing expression of said target RNA, comprising administering to said animal a gap-widened antisense oligonucleotide 18-24 nucleotides in length targeting a preselected RNA target in the liver, wherein said oligonucleotide comprises: (a) a gap region having 12 to 18 contiguous 2′-deoxyribonucleotides; (b) a first wing region having 2 to 4 contiguous nucleosides; and (c) a second wing region having 2 to 4 contiguous nucleosides; wherein the gap region is located between said first wing region and said second wing region and, wherein each nucleoside of said first and second wing regions comprises a 2′-O-(2-methoxyethyl) modification; thereby reducing expression of said preselected RNA target in the liver of said animal.