Patent ID: 8603541

Claim:
A method for preparing a viral-safe platelet extract comprising trophic factors and/or growth factors, the method comprising at least two orthogonal viral inactivation treatments and comprising the following steps: (a) providing a platelet-enriched fraction comprising trophic factors and/or growth factors from multiple donors; (b) preparing a platelet lysate from the platelet enriched fraction in (a); (c) carrying out a solvent-detergent (S/D) viral inactivation treatment of the resultant lysate from step (b); (d) removing the S/D from the resultant viral inactivated platelet lysate of step (c) by hydrophobic interaction chromatography (HIC), wherein the HIC comprises the steps of: loading the resultant lysate from step (c) to HIC resin, and collecting a material eluted under non isocratic conditions from the HIC resin; and conducting a second orthogonal viral inactivation treatment on said eluted material to obtain the viral-safe platelet extract comprising growth factors and/or trophic factors.