Patent ID: 7723563

Claim:
A method of producing one or more peptides or proteins in a transgenic non-human mammal, wherein said mammal comprises multiple exogenous DNA sequences stably integrated in its genome, wherein the method does not require the administration of an exogenous induction factor or ligand, wherein said method comprises: A) providing said transgenic non-human mammal, wherein said mammal comprises multiple exogenous DNA sequences stably integrated in its genome comprising: (1) an exogenous DNA sequence of type A, said type A DNA sequence comprising: (a) 5′ regulatory sequences including a milk protein promoter operably linked to the DNA of (b), (b) DNA encoding a non-secreted transactivating protein, wherein said non-secreted transactivating protein contains a DNA binding domain which recognizes the DNA binding site in a type B DNA sequence, and possesses transactivating activity, and (c) 3′ regulatory sequences active in the transgenic non-human mammal; and (2) one or more exogenous DNA sequences of type B, said type B DNA sequence or each type B DNA sequence comprising: (d) a DNA binding site recognized by the DNA binding domain of said non-secreted transactivating protein, operably linked to a minimal promoter operably linked to the DNA of(e), (e) DNA encoding a peptide or protein, and (f) 3′ regulatory sequences active in the transgenic non-human mammal, wherein said multiple exogenous DNA sequences are effective in directing the secretion of the peptide or protein into milk of said transgenic non-human mammal, wherein said milk protein promoter and said minimal promoter are different promoters; B) allowing the peptide or protein to be produced and secreted into the milk of the transgenic non-human mammal; and C) collecting and purifying the peptide(s) or protein(s).