Patent ID: 7435553

Claim:
A method of detecting and isolating a cell that produces a secreted protein of interest, comprising: (a) constructing a cell line transiently or stably expressing a cell surface capture molecule that binds a protein of interest, by transfecting a cell with a first nucleic acid that encodes said cell surface capture molecule; (b) transfecting the cell line constructed in (a) with a second nucleic acid that encodes the protein of interest, wherein said protein of interest is secreted; (c) detecting the protein of interest displayed on the surface of a cell of the transfected cell line produced in (b) by contacting said transfected cell line produced in (b) with a detection molecule that binds the protein of interest, wherein said contacting with said detection molecule is carried out in the presence of a blocking molecule that binds the cell surface capture molecule and prevents the binding of said secreted protein of interest to said cell surface capture molecule but that does not bind to said detection molecule; (d) isolating the cell bearing the surface displayed protein of interest detected in step (c).