Patent ID: 8569579

Claim:
A Bean Pod mottle virus (BPMV) helper sequence for BPMV vector inoculation in a recipient plant, comprising a BPMV I-Di1 isolate RNA1 sequence that has been modified so that said inoculation produces enhanced symptoms in a recipient plant as compared to a wild type BPMV I-Di1 RNA1, and less severe symptoms as compared to a wild type BPMV K-Ho1 RNA1, and wherein said BPMV I-Di1 isolate RNA1 sequence encodes a polypeptide comprising a BPMV RNA1 helicase having modifications consisting of modifications to the amino acids at position 359 and 365 of the BPMV RNA1 helicase, wherein said amino acid positions are determined by alignment with SEQ ID NO:2 and wherein said BPMV I-Di1 isolate RNA1 sequence encodes a polypeptide having at least 90% identity to SEQ ID NO:8.