Patent ID: 8415313

Claim:
A method of inducing increased production of extracellular IL-10 in mature dendritic cells, comprising (a) exposing a population of human dendritic cells that includes mature dendritic cells to an antisense compound composed of phosphorus-containing intersubunit linkages joining a morpholino nitrogen of one subunit to a 5′ exocyclic carbon of an adjacent subunit and containing 12-40 subunits, including a base sequence of at least 12 contiguous bases that are complementary to a target region within the sequence identified by SEQ ID NO:9, wherein said compound is covalently linked, at one compound end, to an arginine-rich peptide effective to enhance uptake of said compound into the mature dendritic cells, and (b) by said exposing in step (a), blocking expression of full-length CD86 in said cells, (c) by said blocking, thereby producing inhibition of the expression of full-length CD86 on the surface of mature dendritic cells, and enhanced expression of extracellular IL-10 by mature dendritic cells.