Patent ID: 8288114

Claim:
A method for detection of a target compound interacting with a target molecule located on a cell membrane, comprising steps of: combining a first compound with a cell to contact the first compound with the cell, wherein the first compound has a first moiety and a second moiety, wherein the first moiety is capable of binding selectively to the target molecule located on the cell membrane and the second moiety is a radicalization-promoting moiety, and wherein the first moiety of the first compound selectively binds the first compound to the target molecule located on the cell membrane; washing the cell to remove excess first compound; combining a second compound with the cell to contact the second compound with the cell, wherein the second compound has a first group and a second group, wherein the first group is capable of being radicalized by the radicalization-promoting moiety of the first compound and the second group is a labeling group, wherein the first group of the second compound is radicalized by the radicalization-promoting moiety of the first compound, wherein the thus-radicalized first group of the second compound binds to a target compound interacting with the target molecule and existing within 100 nm from the target molecule, and wherein the first group is bound to the second group via a linker; washing the cell to remove excess second compound; and identifying the target compound, using a means for detecting the labeling group of the second compound.