Patent ID: 8580530

Claim:
An in vitro method for identifying a plurality of anti-erythrocyte antibodies in a biological sample, by (i) bringing said biological sample into contact, in a single test receptacle, or in several separate test receptacles, with groups of distinguishable beads, each group of distinguishable beads carrying (1) erythrocytes carrying antigenic molecules to which said anti-erythrocyte antibodies bind or (2) erythrocyte membrane fragments carrying antigenic molecules to which said anti-erythrocyte antibodies bind under conditions which allow the antibodies or activated serum complement fractions present in the sample to bind to said antigenic molecules on the erythrocytes or the erythrocyte membrane fragments, without agglutination, (ii) eliminating the antibodies or activated serum complement fractions which have not bound to said erythrocytes or to said erythrocyte membrane fragments, (iii) labelling the bound antibodies and/or the bound activated serum complement fractions, and (iv) analyzing the mixture so as to identify the group of beads having bound the labelled antibodies or the labelled activated serum complement fractions, thereby allowing the identification of the anti-erythrocyte antibodies.