Patent ID: 7951560

Claim:
A method for the recombinant production of substantially pure Δ4, 5 glycuronidase, comprising: a) introducing into a host cell an isolated nucleic acid molecule encoding a polypeptide having Δ4, 5 glycuronidase activity, wherein the isolated nucleic acid molecule is selected from the group consisting of: (i) nucleic acid molecules which hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence set forth as SEQ ID NO:2, wherein the hybridization conditions are hybridization at 65° C. in hybridization buffer comprising 3.5× (0.15M sodium chloride/0.015M sodium citrate, pH7), 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% bovine serum albumin, 25 mM NaH 2 PO 4 , pH 7, 0.5% sodium dodecylsulphate and 2 mM ethylene diamine tetra acetic acid, (ii) nucleic acid molecules that differ from the nucleic acid molecules of (i) in codon sequence only due to degeneracy of the genetic code, and (iii) full complements of (i) or (ii); b) expressing recombinant Δ4, 5 glycuronidase in the host cell; and c) isolating the recombinant Δ4, 5 glycuronidase from the host cell to produce substantially pure Δ4, 5 glycuronidase.