Patent ID: 8207137

Claim:
A method of treating a mammal with melanoma by decreasing the number of intratumoral vessels associated with the melanoma, or inhibiting the formation of new intratumoral vessels therein, the method comprising administering by: a) injection in the tumor of the mammal of a therapeutically effective amount of an expression plasmid comprising a polynucleotide coding for a therapeutic peptide consisting of SEQ ID NO:2 absent any operably linked coding sequence, wherein the polynucleotide sequence is operably linked to a promoter or an expression control sequence, followed by application of electrical pulses to the site of injection in the tumor of the mammal; or b) injection in a muscle of the mammal of a therapeutically effective amount of an expression plasmid comprising a polynucleotide coding for a therapeutic peptide consisting of SEQ ID NO:2 absent any operably linked coding sequence, wherein the polynucleotide sequence is operably linked to a promoter or an expression control sequence, followed by application of electrical pulses to the site of injection in the muscle of the mammal; whereby the expression of SEQ ID NO:2 decreases the number of intratumoral vessels associated with the melanoma or the formation of new intratumoral vessels therein and the melanoma in the mammal is treated.