Patent ID: 8540946

Claim:
A disposable analysis cartridge capable of performing at least part of a blood sample measurement, comprising: two or more reagent storage reservoirs, a first one for storing a lysing reagent and a second one for storing a sphering reagent; a lysing on the fly channel in fluid communication with the first one of the reagent storage reservoirs, the lysing on the fly channel having a first intersection region that receives a first reagent from the first one of the reagent storage reservoirs and at least part of the blood sample; a sphering on the fly channel in fluid communication with the second one of the reagent storage reservoirs; a first flow cytometry analysis channel structured to hydrodynamically focus a blood sample and to support optical light scattering measurements, the first flow cytometry analysis channel in fluid communication with and downstream of the lysing on the fly channel; and a second flow cytometry analysis channel structured to hydrodynamically focus a blood sample and to support optical light scattering measurements, the second flow cytometry analysis channel in fluid communication with and downstream of the sphering on the fly channel; wherein the first intersection region includes a first end and a second end and wherein a cross-sectional area of the first intersection region tapers from the first end to the second end, the first reagent fluidly connected to the first end of the first intersection region and the at least part of the blood sample entering the first intersection region downstream of the first end such that the first reagent flows circumferentially around the blood sample.