Patent ID: 7659069

Claim:
A method for detecting an analyte in a sample, said method comprising: a. forming a reaction mixture comprising: a first analyte specific probe and a second analyte specific probe, wherein said first analyte specific probe comprises a first binding moiety and a first portion of a first polymerase and wherein said second analyte specific probe comprises a second binding moiety and a second portion of said first polymerase, a modified polynucleotide template, a first primer complementary to said polynucleotide template, a second polymerase, and a second primer complementary to an amplification template; b. incubating said reaction mixture so as to permit: said first and said second binding moieties to bind to said analyte such that said first and said second portions of said first polymerase interact to form a polymerase complex, annealing of said first primer to said modified polynucleotide template, and extending said primer by said polymerase complex thereby synthesizing said amplification template; c. annealing said second primer to said amplification template and extending said primer with said second polymerase and amplifying said amplification template with said second polymerase, wherein said second polymerase is unable to amplify the modified polynucleotide template under the conditions of the first primer extension reaction; and d. detecting said amplified amplification template wherein detection of said amplified amplification template is indicative of the presence or amount of said analyte in a sample.