Patent ID: 8273533

Claim:
A method of preparing a patterned spot microarray comprising: coating a photocatalyst on a substrate to form a photocatalyst layer; coating a composition on the photocatalyst layer to form an organic thin film layer coated on the photocatalyst layer, wherein the organic thin film layer comprises a functional group to be connected to a nucleic acid; spotting the nucleic acid on the organic thin film layer and immobilizing the nucleic acid; positioning a photomask above a spot of the immobilized nucleic acid; and irradiating the spot of the immobilized nucleic acid with ultraviolet light through the photomask to form a pattern on the spot of the immobilized nucleic acid, wherein nucleic acid irradiated with light through the photomask is oxidized to reduce the size of the nucleic acid spot, and wherein target nucleic acid present in a sample does not hybridize to the patterned spot microarray in an area where the nucleic acid has been irradiated with light through the photomask.