Patent ID: 8268561

Claim:
A method for identifying an individual not in need of follow-up testing for T1D, comprising: (a) amplifying DNA in a nucleic acid-containing sample obtained from the individual using oligonucleotide primers specific for exons two of HLA DQB1 and DQA1 loci to provide amplified DNA; (b) simultaneously contacting the amplified DNA with a set of oligonucleotide probes, the set of oligonucleotide probes consisting of: a first oligonucleotide probe specific for DQA1*02, a second oligonucleotide probe specific for DQB1*0301, a third oligonucleotide probe specific for DQB1*0503 and DQB1*0601, a fourth oligonucleotide probe specific for DQB1*0602 and DQB1*0603, a fifth oligonucleotide probe specific for DQB1*0302 and a sixth oligonucleotide probe specific for DQB1*02 for a period of time sufficient for the oligonucleotide probes to hybridize to the amplified DNA; and (c) identifying that the individual is not in need of testing for T1D by the presence of DNA that binds to any one of the first, second, third and fourth oligonucleotide probes or the absence of DNA that binds to at least one of the fifth and sixth oligonucleotide probes.