Patent ID: 7402411

Claim:
A method for identifying a target virus in an infected subject comprising (i) obtaining a total nucleic acid preparation of the infected subject; (ii) preparing a test sample by amplifying the total nucleic acid preparation of the infected subject with a first pair of primers comprising a forward degenerate primer comprising a nucleotide sequence encoding a first highly conserved amino acid sequence of the target virus, and a reverse degenerate primer, the complete complementary sequence of the reverse degenerate primer comprising a nucleotide sequence encoding a second highly conserved amino acid sequence of the target virus; (iii) contacting the test sample with a species-specific probe, the species-specific probe being prepared by amplifying the target virus gene sequence with a second pair of primers comprising: TuMV-F1 of SEQ ID NO: 7, and TuMV-R1 of SEQ ID NO: 15; and (iv) detecting a hybridization between the species-specific probe and the test sample, wherein the hybridization indicates the target virus is identified in the infected subject.