Patent ID: 8679827

Claim:
A method of measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit, said method comprising: contacting said sensor with a liquid sample containing said analyte; contacting said sensor with an enzyme-labeled antibody capable of binding said analyte, whereby a complex of immobilized antibody, analyte and labeled antibody is formed; contacting said sensor with a solution comprising a substrate for said enzyme and at least one air segment to remove unbound analyte and labeled antibody from a region of the sensor; removing substantially all said solution from said sensor while retaining a portion of said solution over said electrode, said counter/reference electrode, and a contiguous portion of a wall of the conduit connecting said electrodes; and detecting a product of a reaction between said enzyme-labeled antibody and said substrate using said sensor, whereby the amount of said analyte in the liquid sample is measured.