Patent ID: 8063270

Claim:
A method for detecting the presence or absence of PVY strains responsible for veinal, foliar or tubercular necrosis in plants of the Solanaceae family, characterized such that it comprises the following steps: a) extraction of nucleic acids from a plant sample, b) RT-PCR amplification of a region of PVY viral RNA comprising: i) codon 738 of SEQ ID NO: 1 or 3, corresponding to amino acid 400 of the HC-Pro protein of SEQ ID NO: 2 or 4, respectively; and ii) codon 757 of SEQ ID NO: 1 or 3, corresponding to amino acid 419 of the HC-Pro protein of SEQ ID NO: 2 or 4, respectively, c) detecting the presence or absence of each of the following: α) a codon encoding the K amino acid at position 400 of the HC-Pro protein; and β) a codon encoding the E amino acid at position 419 of the HC-Pro protein set forth in SEQ ID NO: 2 or 4, the detection of said codons set forth in α) and β) being an indication of a virulent strain of PVY capable of causing necrosis in plants of the Solanaceae family.