Patent ID: 7087388

Claim:
A method of identifying a modulator of signal transduction affected by an Inactivation No Afterpotential D (InaD) protein, comprising: a) contacting a first cell with a test chemical, wherein said first cell comprises at least one signal transduction protein and a polynucleotide encoding a transducisome protein, and said transducisome protein functionally binds to said signal transduction protein to permit or enhance signal transduction, and wherein the transducisome protein is InaD, b) activating said signal transduction in said first cell, c) detecting said signal transduction from said first cell with a signal transduction detection system, d) contacting a second cell with said test chemical, wherein said second cell comprises said signal transduction protein and a polynucleotide encoding a defective InaD protein that fails to functionally bind at least one signal transduction protein to permit or enhance signal transduction, and wherein the defective InaD protein comprises an amino acid mutation in a PDZ domain other than an inaD 215 mutation, e) activating said signal transduction in said second cell, f) detecting said signal transduction from said second cell with a signal transduction detection system, and g) comparing said signal transduction from said first cell with signal transduction from said second cell, wherein a difference in detected signal transduction from said first cell and said second cell identifies said test chemical as a modulator of signal transduction affected by InaD.