Patent ID: 7691255

Claim:
A measuring method for an analyte using a sensor comprising: a sample-holding unit including a space for holding a sample including a first analyte comprising an antigen and a second analyte comprising an ion; a sample-supplying port for supplying said sample to said sample-holding unit, the port being connected to the space; a detecting unit for carrying out an electrochemical measurement, the detecting unit being provided in said sample-holding unit; a light-entrance unit for introducing incident light to said sample-holding unit, the light-entrance unit being provided in said sample-holding unit; a light-exit unit for releasing outgoing light from inside said sample-holding unit to outside said sample-holding unit, the light-exit unit being provided in said sample-holding unit; and a reagent-holding unit for holding a reagent comprising an antibody for said optical measurement, the reagent-holding unit being provided in said sample-holding unit; wherein in a flowing direction of said sample supplied from said sample-supplying port in said sample-holding unit, said sample-supplying port, said detecting unit, and said reagent-holding unit are positioned in the order recited; the method comprising the steps of: (A) supplying said sample to said sample-holding unit; (B) applying a voltage to said detecting unit; (C) measuring an electric-signal from said detecting unit; (D-1) quantifying said second analyte based on said electric-signal measured in said step (C); (D-2) causing antigen-antibody reaction between said first analyte in said sample and said antibody held in said sample-holding unit; (E) applying incident light to said sample held in said sample-holding unit via said light-entrance unit; (F) measuring outgoing light released from inside said sample-holding unit to outside said sample-holding unit via said light-exit unit, caused by the application of said incident light; and (G) quantifying said first analyte based on said outgoing light measured in said step (F) and quanitification result of said second analyte.