Patent ID: 7595184

Claim:
An isolated nitrile hydratase comprising an α-subunit and a β-subunit, wherein: (a) the α-subunit comprises the polypeptide of SEQ ID NO: 1, except that the amino acid sequence of the polypeptide of SEQ ID NO: 1 is substituted at positions: 36 to Met, 71 to His, 148 to Asp, 204 to Arg, Lys, Trp or Thr, 148 and 204 to Asp and Arg respectively, 36, 148 and 204 to Met, Asp and Arg respectively, 36 and 126 to Met and Tyr respectively, 6, 36, and 126 to Met, Thr and Tyr respectively, or 19, 71, and 126 to His, Val and Tyr respectively; and (b) the β-subunit comprises the polypeptide of SEQ ID NO: 2.