Patent ID: 8168052

Claim:
A complex comprising: a polymer; at least one spacer covalently bonded to the polymer; an analyte-responsive enzyme; and a mediator chemically bonded to the at least one spacer, wherein the mediator is a transition metal complex having the formula: wherein M is cobalt, iron, ruthenium, osmium, or vanadium; and L 1 , L 2 , L 3 , L 4 , L 5 , and L 6 are ligands, each of the L 1 , L 2 , L 3 , L 4 , L 5 , and L 6 ligands comprises a heterocycle coordinatively bound to M via a heteroatom of the heterocycle, each of the L 1 , L 2 , L 3 , L 4 , L 5 , and L 6 ligands is independently a monodentate ligand or is combined with at least one other of the L 1 , L 2 , L 3 , L 4 , L 5 , and L 6 ligands to form a multidentate ligand.