Patent ID: 7105307

Claim:
A method of screening for a modulator of an enzyme, comprising the steps of: a) contacting said enzyme, an isolated polypeptide and a binding partner of said polypeptide to form a mixture, wherein said isolated polypeptide comprises a site for post-translational modification, wherein said enzyme catalyzes a reaction at said site of post-translational modification, wherein said reaction results in association of said isolated polypeptide and said binding partner, and wherein each of said isolated polypeptide and said binding partner comprise a detection means for monitoring association of said isolated polypeptide and said binding partner, and wherein each of said isolated polypeptide and said binding partner comprises at least one coiled-coil structure, and wherein said association occurs in a coiled-coil dependent manner; b) contacting the mixture of step (a) with a candidate modulator; and c) monitoring association of said isolated polypeptide and said binding partner in each of steps (a) and (b), wherein a change in association of said isolated polypeptide and said binding partner between step (a) and step (b) identifies the candidate modulator as a modulator of the enzyme.