Patent ID: 7829275

Claim:
A method of detecting a plurality of different target analytes comprising: (a) providing a conductive support comprising a single electrode, wherein said single electrode comprises an array comprising a plurality of different probes, wherein each of the plurality of different probes is attached at one of a plurality of discrete positions; (b) contacting the plurality of different probes with a plurality of different target analytes, wherein each of the plurality of different target analytes is selected from the group consisting of a single stranded DNA oligomer, a single stranded RNA oligomer, a peptide nucleic acid analog, double stranded DNA, an antibody, a polypeptide, a peptide, a target analyte that is reverse transcribed from a nucleic acid sequence, a target analyte comprising intact genomic DNA, a target analyte comprising fragmented genomic DNA, mRNA, a PCR product and an OLA product, further wherein each of the plurality of different target analytes comprises a nanoparticle comprising a photoelectrochemically active moiety, thereby forming a support comprising a plurality of different target analytes attached thereto; (c) irradiating one of the plurality of discrete positions with light individually, thereby generating a photoelectric current between a photoelectrochemically active moiety and the conductive support if a target analyte is present; (d) measuring the photoelectric current to determine the presence or the amount of a target analyte at the discrete position irradiated; and (e) repeating the irradiating and measuring steps for each of a plurality of discrete positions on the support sequentially, thereby determining the presence or the amount of each of a plurality of different target analytes.