Patent ID: 6855556

Claim:
A glucose biosensor comprising at least one mutated glucose/galactose binding protein and at least one reporter group attached to said binding protein, such that said reporter group provides a detectable and reversible signal change when said mutated binding protein is exposed to varying glucose concentrations; wherein said at least one mutated glucose/galactose binding protein comprises at least one amino acid substitution selected from the group consisting of a cysteine at position 11 , a cysteine at position 14 , a cysteine at position 19 , a cysteine at position 43 , a cysteine at position 74 , a cysteine at position 107 , a cysteine at position 110 , a cysteine at position 112 , a cysteine at position 113 , a cysteine at position 137 , a cysteine at position 149 , a cysteine at position 213 , a cysteine at position 216 , a cysteine at position 238 , a cysteine at position 287 , and a cysteine at position 292 , and wherein said detectable and reversible signal change is related to said varying concentrations.