Patent ID: 7794653

Claim:
A method of treating allografts, comprising: (a) providing an ultrasonic treatment apparatus, said treatment apparatus including a sonication tank, a treatment canister rotatably mountable in said sonication tank, a treatment fluid source having a line in fluid communication with, and rotatably coupled to, said treatment canister, said treatment fluid source configured to supply at least one treatment fluid to the interior of said treatment canister supported by said sonication tank, and said line rotatably coupled with said treatment canister configured to allow simultaneous rotation of said treatment canister and exchange of the at least one treatment fluid with said treatment canister while said treatment canister remains in fluid communication with the fluid source, and at least one ultrasonic transducer configured to transmit ultrasonic energy to the interior of said tank and said treatment canister; (b) placing one or more allografts inside said treatment canister; (c) exposing said allografts to the at least one treatment fluid in said treatment canister while applying ultrasonic energy to said allografts and rotating said treatment canister in said sonication tank.