Patent ID: 6841384

Claim:
A multi-chambered roller bottle system having at least three cylindrical chambers comprising, a first cylindrical chamber suitable for use as a cell growth media reservoir having at least four ports where two ports are located at an outside wall, one providing access to an air source and the other providing general access to the first cylindrical chamber, and where the other two ports are located at an inside wall where the first and second cylindrical chambers abut, one port provides access to an air source for the second cylindrical chamber via a duct connecting to the port on the outside wall providing air access and the other port provides controlled fluid communication with the second cylindrical chamber via a control valve; a second cylindrical chamber suitable for cell or tissue growth having at least three ports, two located at the side wall that abuts with the first cylindrical chamber, one port being coextensive with the port providing air access located at the inside side wall of the first cylindrical chamber and the other port aligned with the port of the first cylindrical chamber providing controlled fluid communication, and the third port providing controlled fluid communication with a third cylindrical chamber via a control valve; a third cylindrical chamber suitable for receiving spent media and having at least two ports, one located on an outside wall that abuts with the second cylindrical chamber and provides general access to the third cylindrical chamber and the second port aligned with the third port of the second cylindrical chamber providing controlled fluid communication with the second cylindrical chamber; and a control means for sequentially actuating the valves to produce a controlled fluid flow from each of the respective chambers to the other at predetermined positions of rotation of the chambers; wherein the first, second and third cylindrical chambers are interconnected so as to permit rotation of the cylindrical chambers as a single assembly about a rotational axis and wherein the control of fluid communication permits growth media from the first cylindrical chamber to be introduced to the second cylindrical chamber and spent media from the second cylindrical chamber to be removed from the second cylindrical chamber to the third cylindrical chamber.