Patent ID: 7815868

Claim:
A SBS-formatted microfluidic device comprising: a plate having an upper surface and a lower surface, wherein the geometry of the plate defines an array of interrogation areas, wherein each interrogation area encompasses at least one reaction site; and an elastomeric microfluidic device comprising a network of microfluidic flow channels formed therein in fluid communication with an array of reaction sites configured to mix a plurality of fluids wherein the efficiency of mixing of the fluids is greater than 35%, the network of microfluidic flow channels comprising a set of reagent input channels and a set of sample input channels, the network of microfluidic flow channels further comprising, for each reaction site a reaction chamber; a slug channel in communication with the reaction chamber, one of the reagent input channels, and one of the sample input channels; a first control valve positioned to open and close the slug channel at both its interface with the reaction chamber and its interface with the sample input channel; and a second control valve positioned to open and close the slug channel at its interface with the reagent input channel.