Patent ID: 8728299

Claim:
A method of increasing the performance of a quantitative analyte determination assay comprising: introducing an analyte containing sample having a liquid component to an electrochemical sensor strip, the strip having a base, a first conductor on the base, a second conductor on the base, and at least one first layer on at least the first conductor, wherein the at least one first layer includes a reagent layer including a polymeric binder, an enzyme system, and at most 8 μg/mm 2 of a mediator, wherein the mediator assists in transferring electrons between the analyte containing sample and the first conductor, and wherein the sample provides electrical communication between the first and the second conductors; applying an electric potential between the first and the second conductors in the form of at least 2 read pulses within 3 to 14 seconds; and measuring a current from at least one of the read pulses to provide a quantitative value of the analyte concentration in the sample with increased performance attributable to at least one performance parameter selected from the group consisting of a stability bias of less than ±10% after the strip is stored at 50° C. for 2 weeks, a hematocrit bias of less than ±10% over a 20 to 60% hematocrit range for whole blood samples, an intercept to slope ratio of at most 10 mg/dL, and combinations thereof.