Patent ID: 8802919

Claim:
Aseptic Anopheles -species mosquitoes, said mosquitoes grown from sterilized Anopheles -species eggs in a mosquito rearing chamber with solid walls and airtight seals, said walls enclosing a sterilized interior space and adapted for filtered air passage, and said chamber provided with aseptic larval rearing broth; wherein said Anopheles -species mosquitoes are infected with metabolically active aseptic sporozoite-stage Plasmodium -species parasites selected from the group consisting of P. falciparum, P. vivax, P. ovales , and P. malariae ; wherein said infection results from feeding on a sterile blood meal comprising infectious gametocyte-stage parasite; and wherein said metabolically active sporozoite-stage parasites isolated from said mosquitoes are aseptic and suitable for clinically relevant use in a human vaccine.