Patent ID: 8658136

Claim:
A method of inducing an immune response to one or more antigens of interest in a mammal, comprising: administering to the mammal a genetically engineered bacterium, comprising: nucleic acid sequences encoding the one or more antigens of interest; and nucleic acid sequences encoding one or more factors that inhibit a mammalian type I interferon response; wherein the nucleic acid sequences encoding the one or more antigens of interest are operably linked to a eukaryotic promoter, and the nucleic acid sequences encoding the one or more factors that inhibit a mammalian type I interferon response are operably linked to a eukaryotic promoter or a prokaryotic promoter, and wherein the step of administering is carried out under conditions which allow the genetically engineered bacterium to invade a cell or tissue in the mammal, and which allow the cell or tissue to produce an immune response to the one or more antigens of interest.