Patent ID: 8623667

Claim:
A method for diagnosing liver cancer, the method comprising the steps of: (1) isolating and enriching glycoproteins by treating a sample derived from a subject with lectin; (2) hydrolyzing the glycoproteins of step (1) to prepare glycoproteins-derived polypeptides; (3) concentrating the glycoproteins-derived polypeptides by using a polypeptide selective monoclonal antibody, or a combination of polypeptide-selective antibodies; and, (4) performing a quantitative analysis on the polypeptides of step (3); wherein if the level of a polypeptide selected from the group consisting of: (a) a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11; and, (b) a polypeptide having any molecular weight selected from the group consisting of 2295.2, 2224.1, 1907.0, 1890.9, 1214.7, 1093.6, 1060.6, 972.5, 959.5, 949.5 and 863.5; is higher than the level of the same polypeptide in a sample from a normal individual, identifying the subject as more likely to have, or as having, liver cancer.