Patent ID: 7829298

Claim:
A method of identifying whether a candidate compound is a modulator of a mammalian antilipolytic GPCR, the method comprising: (a) contacting a candidate compound with a mammalian antilipolytic GPCR, wherein the mammalian antilipolytic GPCR comprises an amino acid sequence selected from the group consisting of: (i) SEQ. ID. NO.:24 (hRUP19) (ii) amino acids 2346 of SEQ. ID. NO.:24 (hRUP19); (iii) the amino acid sequence of a G protein-coupled receptor encoded by a polynucleotide comprising a nucleotide sequence, said nucleotide sequence being the sequence obtainable by a process comprising proforming polymerse chain reaction(PCR) on a human DNA sample using specific primers SEQ. ID. NO.:63 and SEQ. ID. NO.:64; (iv) the amino acid sequence of SEQ. ID. NO.:24 (hRUP19) wherein the threonine at amino acid position 219 of SEQ. ID. NO.:24 (hRUP19) is substituted with lysine; (v) amino acids 2-346 of SEQ. ID. NO.:24(hRUP19)wherein the threonine at amino acid position 219 of SEQ. ID. NO.:24 (hRUP19) is substituted with lysine; and (vi) an amino acid sequence that couples to Gi and is at least 90% identical to the amino acid sequence of SEQ. ID. NO.: 24; and (b) determining whether the receptor functionality is modulated in a manner consistent with Gi coupling to said mammalian antilipolytic GPCR, wherein modulation in receptor functionality consistent with Gi coupling is specific to said mammalian antilipolytic GPCR, and wherein a change in receptor functionality is indicative of the candidate compound being a modulator of the mammalian antilipolytic GPCR.