Patent ID: 8137626

Claim:
A microfluidic system comprising: i) an excitation filter; ii) an emission filter; iii) a microfluidic circuit located between the excitation filter and the emission filter, the microfluidic circuit comprising a fluorescence source; iv) a light emitting device to excite the fluorescence source; and v) a detecting arrangement for detecting fluorescence images on the microfluidic circuit; vi) a three-dimensional polymeric structure, the three-dimensional structure being a monolithic polymeric cast of a mold, the monolithic polymeric cast further comprising a fluidic filter element embedded within the polymeric cast, the mold comprising: a top pin; a bottom pin connected with the top pin but separable from the top pin, the bottom pin including an internal pin; a top piece connected with the top pin; and a bottom piece connected with the bottom pin; and wherein the polymeric cast is a microfluidic filtering device, the microfluidic filtering device adapted to filter fluid, when in use, and wherein the microfluidic circuit is adapted to receive, when in use, the filtered fluid.