Patent ID: 7166429

Claim:
Method for detecting a restriction fragment, comprising the steps of: (A) digesting a starting DNA, with one or more specific restriction endonucleases, to fragment said starting DNA into a series of restriction fragments; (B) ligating the restriction fragments thus obtained with at least one double-stranded oligonucleotide adapter, one end of which can ligate with one or both of the ends of the restriction fragments, to thereby produce tagged restriction fragments of the starting DNA; (C) contacting said tagged restriction fragments under hybridising conditions with at least one oligonucleotide primer; (D) amplifying said tagged restriction fragments hybridised with said primers by PCR so as to cause further elongation of the hybridised primers along the restriction fragments of the starting DNA to which said primers hybridised; and (E) identifying or recovering the amplified or elongated DNA fragment thus obtained; in which the detection of the amplified restriction fragments of step E) is carried out by mass spectroscopy, chromatography, or a combination thereof.