Patent ID: 8747291

Claim:
A method for selectively concentrating and collecting constituents of a biologic liquid mixture selected from the group consisting of blood, tissue or tissue components, said constituents having differing specific gravities and being stratifiable in a centrifugal field produced a centrifuge, said method comprising: a) providing a centrifuge having a chamber, an inlet, a first port and a second port, said chamber having a central longitudinal axis and comprising an assembly of a tubular barrel and an end wall arranged to contain a liquid mixture, said first port being located a first radial distance from said central longitudinal axis, said second port being located a second radial distance from said central longitudinal axis, said second radial distance being smaller than said first radial distance; b) introducing said biologic liquid mixture into said chamber; c) rotating said chamber about said central longitudinal axis to produce said centrifugal field, whereupon said constituents of said liquid mixture in said chamber stratify into at least two concentric stratified constituent layers as a function of the differing specific gravities of said constituents and wherein a first one of said at least two concentric stratified constituent layers is present at said first port; d) selectively opening said first port to enable at least a portion of said first one of said concentric stratified constituent layers to be automatically ejected out of said chamber through said first port as a result of pressure built up by said centrifugal field; e) closing said first port upon the detection of a perceptible interface between said first one of said at least two concentric stratified constituent layers and another of said at least two concentric stratified constituent layers and while said first port is closed rotating said chamber about said central longitudinal axis to produce said centrifugal field, whereupon a second one of said at least two stratified constituent layers is present at said second port; and f) selectively opening said second port to enable at least a portion of said second one of said at least two stratified constituent layers to be automatically ejected out of said chamber through said second port as a result of pressure built up by said centrifugal field.