Patent ID: 7446240

Claim:
A method for the production of a porcine nuclear transfer embryo said method comprising at least the steps of: providing at least one enucleated in vivo matured porcine oocyte; providing at least one porcine donor cell or cell nucleus; placing said at least one enucleated oocyte and at least one donor cell or cell nucleus in contact with one another to form a couplet; incubating said couplet in a first medium which is substantially free of calcium for a period of at least 15 minutes; providing a second medium which is substantially free of calcium; fusing via electrofusion, in said second medium, said couplet to produce a nuclear transfer embryo; providing a third medium which is substantially free of calcium; and incubating said nuclear transfer embryo in said third medium for a period at least 15 minutes following electrofusion.