Patent ID: 7279286

Claim:
A method for detecting the phosphorylation of a substrate molecule by a protein kinase comprising the steps of: a] providing at least one protein kinase, b] providing at least one substrate molecule of said protein kinase wherein said substrate molecule consists of a peptide consisting of amino acid sequence SEQ ID NO: 1, c] proving at least one donor component which is able to bind to said substrate molecule and which emits a signal after said donor component is irradiated by a light source, d] providing at least one acceptor component which is able to bind to said substrate molecule if said substrate has been phosphorylated and is able to receive a signal from said donor component and emit the signal in the form of an electromagnetic radiation, e] bringing said protein kinase, said substrate molecule, said donor component and said acceptor component in contact with each other, f] irradiating the mixture of step e] with a light source in such a way that said donor component is excited and, g] measuring the electromagnetic radiation which is emitted by said mixture.