Patent ID: 8293485

Claim:
A method of analyzing a tissue proteome for the presence of one or more proteins comprising the steps of: (a) contacting a first histopathologically processed biological sample with a first buffer solution and a second histopathologically processed biological sample with a second buffer solution; (b) heating said first histopathologically processed biological sample and second histopathologically processed biological sample at a temperature and for a time sufficient to substantially reduce the protein cross-links present in the biological samples to form heated first and second samples; (c) treating the heated first and second samples with an effective amount of one or more proteolytic enzymes for a time sufficient to substantially reduce the proteins to individual peptides; (d) following treatment with said one or more proteolytic enzymes, separating the heated first sample using a first separation system and separating the heated second sample using a second separation system, wherein the first separation system and the second separation system comprise a chromatography matrix, thereby producing fractions of said first sample and fractions of said second sample; and (e) detecting the presence of at least one protein analyte from Table 1 by the presence of one or more individual peptide(s) produced by the action of said one or more proteolytic enzymes in said fractions of said first sample and said fractions of said second sample, thereby analyzing a tissue proteome.