Patent ID: 7429691

Claim:
A method for producing a transformed maize plant comprising; (a) inducing a shoot meristem culture by culturing on a meristem proliferation medium an isolated maize tissue selected from the group consisting of vegetative shoot meristem tissue, meristem tissue from axillary shoot, stem tissue, or leaf base tissue; (b) introducing a nucleic acid into at least one cell of the shoot meristem culture; (c) selecting the transformed cells; and (d) growing the transformed cells in a regeneration medium so as to produce a transformed plant, wherein the meristem proliferation medium comprises from 0 mg/L to about 3 mg/L of an auxin, from about 2 mg/L to about 8 mg/L of a cytokinin, from about 10 g/L to about 60 g/L of maltose or about 10 g/L to about 60 g/L of sucrose; from about 0.1 μM to about 50 μM copper; and greater than about 35 μM zinc.