Patent ID: 6958233

Claim:
A process for the preparation of a pharmaceutically acceptable salt of a mixture of (R)-S-adenosyl-L-methionine and (S)-S-adenosyl-L-methionine from enriched yeast containing at least 6 g/kg of (R)-S-adenosyl-L-methionine and (S)-S-adenosyl-L-methionine, wherein the process is performed at a temperature of 0-12° C., comprising: (a) adjusting the yeast to a pH of 1.0 to 3.5, (b) lysing the yeast to produce a lysate, (c) microfiltrating the lysate to produce a microfiltrate, (d) adsorbing the microfiltrate on a weak acid resin and eluting with 0.1-2 N inorganic acid to produce an eluate, (e) decolorizing the eluate to produce a decolorized eluate, (f) concentrating the decolorized eluate by reverse osmosis to 30-70% by volume to produce a concentrated, decolorized eluate, (g) adding stoichiometric amounts of a pharmaceutically acceptable acid to the concentrated, decolorized eluate to produce a pharmaceutically acceptable salt of the mixture of (R)-S-adenosyl-L-methionine and (S)-S-adenosyl-L-methionine, wherein the salified (R)-S-adenosyl-L-methionine is present in amounts lower than or equal to 3% with respect to the (S)-S-adenosyl-L-methionine.