Patent ID: 8012744

Claim:
A fluidic device having a housing and being configured to run an immunoassay for detecting an analyte in a sample of bodily fluid within said housing comprising: (a) a sample collection unit adapted to provide a sample of bodily fluid into said fluidic device; (b) an assay assembly in fluidic communication with said sample collection unit, said assay assembly comprising: (i) a reagent chamber comprising at least one reagent used in said assay; and (ii) at least one reaction site comprising a reactant that binds said analyte, wherein said assay assembly is adapted to yield a luminescent signal indicative of the presence of the analyte in the sample of bodily fluid, and wherein the at least one reagent reacts with a second reagent in said assay assembly to produce an optical signal; and (c) a quencher assembly, in fluidic communication with but separated by a channel from said assay assembly, comprising a quenching agent that inhibits an enzymatic reaction between the at least one reagent and the second reagent in said quencher assembly, thereby reducing interference with said luminescent signal.