Patent ID: 8486139

Claim:
A method of preparing a decellularized soft tissue in which soft tissue of animal origin is decellularized, the method comprising: an application step of disrupting cells in the soft tissue by applying an ultrahigh hydrostatic pressure to an isolated soft tissue in a medium; and a removal step of removing the disrupted cells, wherein the medium is an aqueous solution containing a water-soluble polysaccharide; wherein the ultrahigh hydrostatic pressure is at least 1000 atmospheres, wherein the application step comprises a pressure limiting step of limiting the rate of pressure increase or pressure decrease in the applied pressure applied to the medium to inhibit solidification of the medium and damage to the structure of the decellularized tissue during the process of the pressure increase or pressure decrease by preventing the applied pressure applied to the medium from taking a value greater than or equal to a melt pressure of the medium that is calculated in advance based on the composition of the medium.