Patent ID: 7348184

Claim:
A method for creating a profile of interactions between components of at least one multicomponent biological complex in a sample, the method comprising, for each complex: (a) providing an aliquot from the sample, wherein the aliquot comprises the multicomponent biological complex; (b) immobilizing the multicomponent biological complex on a solid support through a biospecific affinity molecule, wherein the affinity molecule is not a nucleic acid, and wherein the affinity molecule binds a first component of the complex and wherein unbound material has been removed from the solid support; (c) washing the immobilized multicomponent biological complex with a sequence of elution washes, wherein a first solute in each elution wash, has a concentration such that the sequence of elation washes forms a gradient of increasing or decreasing concentration of the first solute; and (d) measuring for a second component of the complex in each of the elution washes; whereby the profile for the complex from the sample comprises the measurements from the elution washes.