Patent ID: 8030542

Claim:
A method for decreasing or preventing accumulation of a δ-endotoxin in a plant cell or plant tissue of interest, comprising: a) transforming a plant with a first nucleotide comprising in operable linkage a constitutive plant functional promoter linked to a first polynucleotide encoding a plastid transit peptide and a second polynucleotide encoding said δ-endotoxin in combination with a second nucleotide comprising in operable linkage a tissue-specific promoter linked to an antisense mRNA of said second polynucleotide encoding said δ-endotoxin, wherein said tissue-specific promoter is specific for said plant cell or plant tissue of interest; and b) expressing said second polynucleotide and said antisense mRNA in said plant, wherein said second nucleotide is expressed in said plant cell or plant tissue of interest, thereby resulting in decreased or prevented accumulation of said δ-endotoxin in said plant cell or plant tissue of interest.