Patent ID: 7321832

Claim:
A method of designing a probe from a polynucleotide group comprising a plurality of polynucleotides, the method comprising: providing a sequence for each polynucleotide in a plurality of polynucleotides; forming a polynucleotide group consisting of all the sequences; producing polynucleotide fragments having a certain length from each of the sequences of the polynucleotide group; obtaining a sequence for each polynucleotide fragment; providing, for each polynucleotide fragment, a sequence specific identification number using the sequence for the polynucleotide fragment; comparing the sequence specific identification numbers of the polynucleotide fragments to each other to select a probe; and displaying the selected probe; wherein the sequence specific identification number is calculated by providing bases of nucleotides A, T, G, and C in the sequence of the polynucleotide fragment with values of 0, 4 (L−i) /4, 4 (L−i) /2, and 4 (L−i) ×(3/4), respectively, where L is the length of the polynucleotide fragment and i sequentially increases from i=0 at a 5′-end nucleotide of the polynucleotide to i=L−1 at a 3′-end nucleotide of the polynucleotide fragment; adding the provided values for each base in the sequence and values from 4 1 to obtain a sum; and providing the sum as the sequence specific identification number of the polynucleotide fragment.