Patent ID: 8722860

Claim:
A monoclonal antibody or a binding fragment thereof which: (a) specifically binds to human TNF-α; (b) competes for binding to human TNF-α with an antibody comprising a V H sequence of SEQ ID NO:2 and a V L sequence of SEQ ID NO:4; and (c) comprises six CDRs that have one or more amino acid substitutions or combinations of amino acid substitutions as compared to the CDRs of SEQ ID NO:5 (CDR-H1), SEQ ID NO:6 (CDR-H2), SEQ ID NO:7 (CDR-H3), SEQ ID NO:8 (CDR-L1), SEQ ID NO:9 (CDR-L2) and SEQ ID NO:10 (CDR-L3), wherein said one or more amino acid substitutions or combinations of substitutions are selected from: (1) the CDR-L1 substitutions R7Q; A11S; R7Q+A11S; N8T; N8T+A11S; I6T; A11G; I6T+A11G; Q4G; Q4G+A11S; Q4G+A11G; Q4H; Q4H+A11S; Q4R; Q4R+A11S; G5S+A11S; N8S+A11S; I6T+A11S; and N8T+A11G; (2) the CDR-L2 substitutions S3K or S3R; T4H, T4Q, T4F, T4W or T4Y; L5R or L5K; and Q6R; (3) the CDR-H1 substitutions: Y2H and A3G; and (4) the CDR-H2 substitution: T3N.