Patent ID: 7696335

Claim:
A kit for carrying out multiple non-cross reacting recombination reactions, the kit comprising at least one pair of mutant loxP recombination elements selected from the group of pairs defined by the formula: a first member of a pair is defined in a 5′→3′ orientation as: LE 1 -S 1 -RE 1 and a second member of the pair is defined in a 5′→3′ orientation as: LE 2 -S 2 -RE 2 where: LE 1 is a mutant or wild type left inverted repeat of a loxP recombination element and RE 1 is a mutant or wild type right inverted repeat of a loxP recombination element such that whenever LE 1 is a wild type sequence, RE 1 is a mutant sequence, and whenever LE 1 is a mutant sequence, RE 1 is a wild type sequence; LE 2 is a mutant or wild type left inverted repeat of a loxP recombination element and RE 2 is a mutant or wild type right inverted repeat of a loxP recombination element such that whenever LE 2 is a wild type sequence, RE 2 is a mutant sequence, and whenever LE 2 is a mutant sequence, RE 2 is a wild type sequence; with the proviso that whenever LE 1 is a mutant sequence, then LE 2 is a wild type sequence and whenever LE 1 is a wild type sequence, then LE 2 is a mutant sequence; and S 1 and S 2 are each a mutant loxP spacer region each having the same sequence in a 5′→3′ orientation selected from the group consisting of: GTATAGTA, GGCTATAG, TCGTAGGC, GTGTATTT, GTGTACGG, GCGTATGT, TTGTATGG, GGATAGTA, AGGTATGC, GGTTACGG, TTTTAGGT, GAGTACGC, and GTGTACGC, or, S 1 is GTGTACGC whenever S 2 is GTGTACGG, and S 2 is GTGTACGC whenever S 1 is GTGTACGG.