Patent ID: 8841097

Claim:
A process for producing an unsaturated fatty acid-containing oil comprising: (a) culturing a microorganism belonging to the genus Mortierella subgenus Mortierella in a liquid medium containing a nitrogen source derived from soybean in a fermenter, wherein the ratio of the nitrogen source derived from soybean with respect to the total nitrogen source in the medium is at least 86%, and (b) collecting the unsaturated fatty acid-containing oil from the cultured product, wherein the unsaturated fatty acid-containing oil has a 24,25-methylenecholest-5-en-3β-ol compositional ratio of 35% or lower, a 24,25-methylenecholest-5-en-3β-ol compositional ratio in a proportion of 1.2 or less with respect to the desmosterol compositional ratio, and an arachidonic acid content of 30 to 50%, and wherein the culture step is carried out while maintaining i) a glucose concentration within the range of 0.5 to 5 wt % during the first 3 days of culturing, and/or ii) an average glucose concentration within the range of 0.7 to 3 wt % during the first 3 days of culturing.