Patent ID: 7378259

Claim:
A method of synthesizing a polynucleotide sequence comprising: providing a fluid processing device comprising a reaction chamber and first and second input openings in fluid communication with the reaction chamber; introducing a protected first nucleotide monomer into the second input opening of a fluid processing device; moving the protected first nucleotide monomer by capillary action from the second input opening into the reaction chamber; attaching the protected first nucleotide monomer to a support disposed in the reaction chamber to form a protected first supported nucleotide monomer; introducing a first deprotecting reagent into the first input opening; moving the first deprotecting reagent by capillaiy action from the first input opening into the reaction chamber to form a deprotected first supported nucleotide monomer in the reaction chamber; introducing a wash reagent into the first input opening; moving the wash reagent by capillary action from the first input opening into the reaction chamber; moving the first deprotecting reagent out of the reaction chamber; introducing a protected second nucleotide monomer into the second input opening; moving the protected second nucleotide monomer by capillary action from the second input opening into the reaction chamber; and contacting the protected second nucleotide monomer with the deprotected first nucleotide monomer in the reaction chamber to form a supported polynucleotide sequence.