Patent ID: 6848462

Claim:
A method for fabricating a microfluidic device, the method comprising the steps of: providing a first substantially flat platen and a second substantially flat platen; providing a plurality of substantially planar, substantially metal-free, adhesiveless polymer device layers including: a first cover layer and a second cover layer, at least one of the first cover layer and the second cover layer defining a fluidic port; and at least one stencil layer defining a microfluidic channel penetrating through the entire thickness of the at least one stencil layer, the microfluidic channel being bounded laterally by the at least one stencil layer, and being bounded from above and below by additional device layers of the plurality of device layers to define an upper channel surface and a lower channel surface; stacking the plurality of device layers between the first platen and the second platen; and controllably heating the plurality of stacked device layers according to a heating profile adapted to form a substantially sealed adhesiveless microfluidic device wherein the upper channel surface remains distinct from the lower channel surface.