Patent ID: 7772383

Claim:
A composition for performing a polynucleotide replication reaction, which comprises a buffer, one or more template polynucleotides, nucleotide triphosphates, one or more polymerase enzymes or fragments thereof, and at least one organic diol having the formula C n H 2n+2 O 2 , wherein n is an integer with value equal to or greater than three but less than or equal to six, wherein when n=3, the diol is 1, 3 propanediol, when n=4 the diol is 1,2-butanediol or 1,3-butanediol, and when n=6, the diol is 1,6-hexanediol or 1,2-hexanediol or 2-methyl-2,4-pentanediol, wherein said organic diol is proportioned to the other ingredients in a ratio that provides an effective concentration range greater than 0.01 molar but less than 2.5 molar of said organic diol, and wherein said composition is functional to produce a detectable amount of product in a polynucleotide replication reaction at a denaturing temperature lower than 95° C. and an extension temperature lower than 72° C.