Patent ID: 8809514

Claim:
A tripartite oligonucleotide complex comprising: i) a first oligonucleotide between 29 nucleotides and 37 nucleotides in length having a target-dependent region and a target-independent region that is 5′ to the target-dependent region, ii) a second oligonucleotide between 19 nucleotides and 23 nucleotides in length comprising an antisense region that has greater than 70% complementarity to a target gene of an organism, and iii) a third oligonucleotide between 10 nucleotides and 14 nucleotides in length, wherein the target-dependent region of the first oligonucleotide is greater than 80% complementary to the second oligonucleotide and the target-independent region of the first oligonucleotide is greater than 80% complementary to the third oligonucleotide, such that the first, second and third oligonucleotides are capable of forming a tripartite complex having at least two regions of duplex, and the third oligonucleotide is a non-targeting sequence.