Patent ID: 8912511

Claim:
A device for multi-photon fluorescence microscopy for obtaining information from biological tissue, comprising: a laser unit for generating an excitation radiation, an optical unit which is formed to focus the excitation radiation for generating an optical signal at different locations in or on an object to be examined, a control and processing unit, a patient module connected to the control and the processing unit, which for examining the object can be placed relative to the object, wherein the optical is part of the patient module, and a detector module for detecting the optical signal from the region of the object, wherein the optical unit for generating the optical signal at different locations in or on the object is movable within a housing of the patient module in at least one direction relative to the object, wherein the laser unit is part of the control and processing unit and an optical fiber connects the laser unit with the patient module for transmitting the excitation radiation towards the optical unit, wherein the laser unit generates an excitation radiation with a first wavelength to transmit the excitation radiation through the optical fiber, and a frequency doubler is arranged in the patient module for halving the wavelength of the excitation radiation prior to the optical unit, wherein from the received optical signal is a sectional image of the object is generated pixel by pixel at different locations of the object, wherein for generating the sectional image pixel by pixel the object is exposed to the excitation radiation in a triggered manner, and wherein the pixel size of the sectional image pixel by pixel is determined in horizontal direction by the focus of the excitation radiation and by adjusting the triggering, and in vertical direction by the waist length of the focused excitation radiation, wherein the pixel size is adjustable by a beam expansion of the excitation radiation and by adjusting the triggering.