Patent ID: 8735059

Claim:
An assay kit for high-throughput whole genome haplotyping of a subject, comprising: (a) reagents for single cell collection and cytogenetic staining; (b) reagents for single cell genomic DNA amplification and genome genotyping, and (c) a non-transitory computer readable medium having computer-executable instructions facilitating a determination of a subject's haplotype, wherein each single cell lysate comprises a partial set of the total chromosomes present in the single cell of the subject from which the lysate was derived, wherein the computer-executable instructions are configured to: (1) assemble an allelic genotype from nucleotide sequence information obtained from a subset of a subject's chromosomal DNA to produce a first set of genotyping data; (2) assemble an allelic genotype from nucleotide sequence information obtained from the subject's total genomic DNA; (3) determine a haplotype of alleles in said subject based on a comparison of the allelic genotypes in steps (1) and (2); and (4) repeat steps (1) to (3) to determine the haplotypes of additional alleles.