Patent ID: 6949368

Claim:
A composition for performing a polynucleotide replication reaction, which comprises a buffer, one or more template polynucleotides, nucleotide triphosphates, one or more polymerase enzymes or fragments thereof, and one or more reaction adjuvants comprising compounds of the formula: wherein: R 1 is C or S; and when R 1 is C, X is ═O, R 3 is N and R 6 is absent; when R 1 is S, X is ═O or and R 3 is C; R 2 is H or CH 3 only when one or more of R 4 , R 5 and R 6 is not H, and otherwise R 2 is an unsubstituted or halogen-, hydroxy- or alkoxy- substituted alkyl or cycloalkyl of length m, wherein m is selected such that the total number of carbons in the compound is between 3 and 8 when R 1 is C and between 2 and 8 when R 1 is S; wherein any two of R 2 , R 3 , R 4 , R 5 and R 6 optionally form a cyclic structure in which cyclization is effected through a bond between them; and R 4 , R 5 and R 6 each is H, alkyl, cycloalkyl or halogen-, hydroxy- or alkoxy- substituted alkyl or cycloalkyl of length n, wherein n is selected such that the total number of carbons in the compound is between 3 and 8 when R 1 is C and between 2 and 8 when R 1 is S and when R 4 and R 5 are CH 3 , R 2 cannot be H or CH 3 .