Patent ID: 7527935

Claim:
A method for screening or identifying an antagonist of an eicosanoid receptor protein, comprising: (1) bringing the receptor protein into contact with a test compound and a ligand; (2) detecting the function or the activity of the receptor protein; and (3) determining whether or not the test compound has an ability to inhibit the function or the activity of the receptor protein, or determining an intensity of the ability: wherein the function or the activity of the receptor protein is selected from the following (i), (ii) and (iii), (i) specific binding to a ligand, (ii) induction of intracellular signal transductions based on a stimulation by a ligand, and (iii) activation of G-protein based on a stimulation by a ligand; where the ligand is an eicosanoid; and said receptor protein is a polypeptide selected from (A) and (B); (A) a polypeptide comprising an amino acid sequence shown by SEQ ID NO:2 or SEQ ID NO:21; and (B) a polypeptide encoded by a nucleic acid which hybridizes under highly stringent condition with a nucleic acid comprising a nucleotide sequence shown by SEQ ID NO:1 or SEQ ID NO:20 and having a function or activity as a receptor of an eicosanoid; said hybridization under highly stringent condition being carried out by conducting hybridization for 16 hours at a temperature of 50 to 60° C. in 6×SSC or in a hybridization solution having an equivalent salt concentration to 6×SSC, followed by preliminary washing with 6×SSC or with a solution having an equivalent salt concentration to 6×SSC, and subsequently followed by washing in 0.1×SSC or in a solution having an equivalent salt concentration to 0.1 SSC; and said receptor protein is in a form of a cell over-expressing the receptor protein by introducing an exogenous nucleic acid encoding the receptor protein or by introducing an exogenous expression vector comprising the same.