Patent ID: 7396676

Claim:
A method of making a sensor, the method comprising contacting a first reactive moiety having the structure R1-N═C═Y1 with a second reactive moiety having the structure R2-X1H m under conditions sufficient to result in a compound having the structure wherein: one of R1 or R2 is a sensor substrate; the other of R1 or R2 is selected from a ligand, a linking group bound to a ligand, a functional group for binding a ligand, or a linking group bound to a functional group for binding a ligand; Y1 is selected from O or S; X1 is selected from N or S, provided that, if Y1 is S, X1 is N; n equals 1 when X1 is N, or n equals 0 when X1 is S; and m equals n plus 1.