Patent ID: 7416890

Claim:
A method of producing a baculovirus vector comprising, cotransfecting, and amplifying, wherein said cotransfecting comprises cotransfecting insect cells with at least a plasmid containing a gene coding a first protein capable of being expressed on a cell surface and a recombinant baculovirus DNA, wherein a first pseudotyped baculovirus is generated which comprises at least one part of the baculovirus DNA and which is coated with the first protein capable of being expressed on a cell surface, wherein said amplifying comprises infecting cells which express a plasmid containing a gene coding a second protein capable of being expressed on a cell surface with the first pseudotyped baculovirus; and amplifying the virus DNA to thereby generate a second pseudotyped baculovirus which is coated with the second protein capable of being expressed on a cell surface, and wherein, in the recombinant baculovirus DNA, a polyhedrin gene is replaced with a first foreign gene.