Patent ID: 7879799

Claim:
A method to produce an immunizing peptide for generating an antibody specific for a glycopolypeptide, comprising (a) isolating a glycosylated portion of said glycopolypeptide by treating said glycopolypeptide with periodate, followed by treating with biocytin hydrazine, and coupling the resultant to streptavidin; (b) identifying by mass spectroscopy an amino acid sequence consisting of 6-40 amino acids whose C-terminus or N-terminus is within 5 amino acids upstream or downstream respectively of the N-terminus or C-terminus respectively of a glycosylated site of said glycosylated portion of glycopolypeptide obtained in step (a), wherein said site having the sequence N—X—S/T; (c) producing said immunizing peptide corresponding to the amino acid sequence identified in step (b).