Patent ID: 8436224

Claim:
A method of determining effects of administering a Caloric Restriction (CR) mimetic or a CR mimetic candidate to a non-mammalian transgenic organism, the method comprising: administering at least one of the CR mimetic or the CR mimetic candidate to the non-mammalian transgenic organism, the non-mammalian transgenic organism comprising a transgene designed to produce a disease or ailment in the non-mammalian transgenic organism; determining the effects of the administering wherein the determining comprises determining whether the administering improves the disease or ailment; and performing a plurality of other tests using the at least one of the CR mimetic or the CR mimetic candidate, wherein at least one of the plurality of other tests comprises determining a protective effect of the CR mimetic or the CR mimetic candidate against oxidative damage to mitochondria by (1) inducing oxidative damage to the mitochondria, (2) administering an amount of the CR mimetic or the CR mimetic candidate sufficient to inhibit the induced oxidative damage in the mitochondria, and (3) evaluating the effect of the CR mimetic or the CR mimetic candidate on mitochondrial complex activity.