Patent ID: 7803544

Claim:
A method comprising detecting an amplification product by hybridizing it with a probe nucleic acid, wherein the amplification product is amplified from a target nucleic acid with primers, wherein the target nucleic acid comprises F3, F2 and F1 regions in this order from a 5′ terminal side; B3c, B2c and B1c regions in this order from a 3′ terminal side; an FP region in the region from the F2 to F1 regions and/or a BPc region in the region from the B2c to B1c regions; and further comprises at least one of an LF region in the region from F2 to F1 regions and an LBc region in the region from the B2c to B1c regions; wherein the probe nucleic acid comprises a nucleotide sequence complementary to a sequence selected from the group consisting of sequence in the FP and BPc region and sequences complementary thereto; wherein the primers include an FIP primer comprising sequence identical to that of the F2 region at the 3′ terminal side and a sequence complementary to that of the F1 region at the 5′ terminal side; an F3 primer comprising sequence identical to that of the F3 region; a BIP primer comprising sequence complementary to that of the B2c region at the 3′ terminal side and sequence identical to that of the B1c region at the 5′ terminal side; a B3 primer comprising sequence complementary to that of the B3c region; and at least one of a loop primer LFc comprising sequence complementary to that of the LF region and a loop primer LBc comprising sequence identical to that of the LBc region; and wherein the primers are designed in such a manner that the FP and F2 regions and/or the BPc and B2c regions have a non-overlapping region of at least 10 bases or more and overlapping regions of 10 bases or less and that the FP and LF regions and/or the BPc and LBc regions have a non-overlapping region of at least 10 bases or more and overlapping regions of 10 bases or less.