Patent ID: 8067149

Claim:
An acellular tissue matrix made from dermal tissue comprising: (a) an acellular reticular dermal matrix comprising collagen, wherein the acellular reticular dermal matrix comprises an extracellular protein and collagen matrix; and (b) an intact basement membrane, wherein the basement membrane comprises a structurally preserved lamina densa and anchoring fibrils made by the process comprising: de-epidermizing the dermal tissue with 1 M sodium chloride at 37±2° C. for 16-32 hours for human dermal tissue, or 35-55 hours for porcine dermal tissue, removing the epidermis from the dermis, rinsing the dermis with Hank's balanced salt solution at 20-26° C., de-cellularizing the dermis with 0.5% sodium dodecyl sulfate for 1 hour at 22-26° C., without the use of enzymatic treatment, rinsing the dermis with Hank's balanced salt solution for 5 minutes at 20-26° C., thereby providing an acellular dermal matrix, wherein the acellular dermal matrix is not subjected to a cross-linking agent.