Patent ID: 8460916

Claim:
A nucleic acid encoding a variant of a parent AmyTS23 alpha-amylase selected from the group consisting of: (A) a first variant of a parent AmyTS23 alpha-amylase having the amino acid sequence of SEQ ID NO: 1, wherein the first variant has a truncation of the C-terminus and comprises an amino acid sequence with at least 95% identity to the amino acid sequence of SEQ ID NO: 2, provided said first variant has the N-terminal amino acid sequence of Asn-Thr-Ala as depicted in SEQ ID NO: 2, and wherein the first variant has improved alpha-amylase activity at 20° C. relative to said parent and (B) a second variant of a parent AmyTS23 alpha-amylase, wherein said second variant is encoded by a nucleic acid sequence that is complementary to a sequence that is capable of hybridizing under stringent conditions to an isolated nucleic acid encoding said first variant of a parent AmyTS23 alpha-amylase.