Patent ID: 7557125

Claim:
A method for screening for an inhibitor of Fab I or Fab K which comprises: (i) providing a reaction mixture comprising Fab I or Fab K, crotonyl-ACP and NAPDH or NADH; (ii) contacting a candidate compound to said reaction mixture; and (iii) detecting inhibition of Fab I or Fab K wherein said candidate compound is represented by formula (I): wherein: R 1 is H or C 1-4 alkyl; R 2 is H, C 1-4 alkyl or C 3-6 cycloalkyl; R 4 is H or C 1-4 alkyl; indicates that one of the two designated bonds is a double bond and the other is a single bond; R 5 is CH 2 when the bond to which it is attached is a double bond; or R 5 is H or C 1-4 alkyl when the bond to which it is attached is a single bond; R 6 is H or C 1-4 alkyl; R 7 is H, C 1-6 alkyl or —C 0-6 alkyl-Ar; Y is H, C 1-4 alkyl, N(R′) 2 , NHC(O)R′, NHCH 2 C(O)R′ or NHC(O)CH═CHR′; each X independently is H, C 1-4 alkyl, CH 2 OH, OR′, SR′, CN, N(R′) 2 , CH2N(R′) 2 , NO 2 , CF 3 , CO 2 R′, CON(R′) 2 , COR′, NR′C(O)R′, F, Cl, Br, I or —S(O) r CF 3 ; W is S or O; Q is H or C 1-4 alkyl; M is CH 2 or O; L is CH 2 or C(O); E is O or NR′; each R′ independently is H, C 1-6 alkyl or —C 0-6 alkyl-Ar; and r is 0, 1 or 2; or a pharmaceutically acceptable salt thereof.