Patent ID: 8236294

Claim:
A method of providing gene therapy to an animal or a human to treat a disease or a condition comprising: administering to the animal or the human a transposon-based vector in an acceptable carrier, the transposon-based vector comprising an isolated polynucleotide sequence encoding: a) a gene operably linked to a first promoter, the gene encoding for a transposase; and, b) one or more genes of interest operably-linked to one or more additional promoters, wherein the one or more genes of interest and their operably-linked promoters are flanked by transposase insertion sequences recognized by the transposase, and wherein the first promoter and the one or more additional promoters are cell-specific promoters or constitutive promoters, wherein the one or more genes of interest codes for production of a peptide comprising a lytic peptide, wherein the disease or condition is cancer and the cancer cells have a receptor for GnRH or LH, and wherein the transposon-based vector in the acceptable carrier is administered directly to the cancer cells.