Patent ID: 7365173

Claim:
A method for producing a composition comprising a purified butyrylcholinesterase free of active virus wherein the first step is contacting a butyrylcholinesterase-containing biological fluid with an ion exchange chromatography material consisting of a cation exchange chromatography material and allowing the butyrylcholinesterase to flow through the cation exchange chromatography material wherein said first step is followed by the subsequent steps comprising steps a through d and wherein step a may optionally follow steps b and c: a. solvent-detergent treating the butyrylcholinesterase-containing fall-through; b. contacting the butyrylcholinesterase-containing fall-through with a butyrylcholinesterase-binding affinity chromatography material; c. recovering bound butyrylcholinesterase from the affinity chromatography material; and d. pasteurized the recovered butyrylcholinesterase thereby obtaining a composition comprising a purified butyrylcholinesterase free of active virus.