Patent ID: 7074564

Claim:
A method comprising: providing a first affinity probe for a target which first affinity probe carries a polynucleotide chain including a first polynucleotide sequence, a second affinicy probe for the target which second affinity probe carries a polynucleotide chain including a second polynucleotide sequence, a padlock probe provided in two parts wherein the first part of the padlock probe has a 5â€² end which is complementary to the first polynucleotide sequence and a 3â€² end complementary to the second polynucleotide sequence, and the second part of the padlock probe has a 3â€² end which is complementary to the first polynucleotide sequence and a 5â€² end complementary to the second polynucleotide sequence; binding the first affinity probe to the target; binding the second affinity probe to the target; hybridizing the padlock probe provided in two parts; circularizing the padlock probe; and effecting amplification of the padlock probe.