Patent ID: 7666663

Claim:
A method of correcting a distribution of a quantity of fluorescent light on a biochip using a biochip reader comprising a camera, the method comprising: mounting a uniform fluorescent plate in said biochip reader; emitting excitation light from a light source and irradiating said uniform fluorescent plate thereby producing fluorescent images of said uniform fluorescent plate; obtaining a plurality of intensity values of fluorescent images of said uniform fluorescent plate by measuring the intensity of each individual pixel of said uniform fluorescent plate with said camera, each of the plurality of intensity values of said uniform fluorescent plate representing the intensity value of one individual pixel of said uniform fluorescent plate; obtaining an average value of reference quantity of fluorescent light distribution image from said uniform fluorescent plate by averaging said plurality of intensity values of fluorescent images of said uniform fluorescent plate; mounting a biochip comprising measurement samples in said biochip reader in lieu of said uniform fluorescent plate, said uniform fluorescent plate having an area equivalent to the area comprising said measurement samples on said biochip; emitting excitation light from said light source and irradiating said biochip, said measurement samples on said biochip being capable of emitting fluorescent light in response to the irradiation with said excitation light; obtaining a plurality of intensity values of fluorescent images from said biochip by measuring intensity of each individual pixel of said measurement samples on said biochip with said camera, each of the plurality of intensity values of said biochip representing the intensity value of one individual pixel of said measurement samples on said biochip; and obtaining a fluorescent light source intensity corrected sample image value for each individual pixel of said biochip, thereby correcting the distribution of the quantity of fluorescent light on said biochip according to the following formulas: a i ′=a i ÷a Ave (I) c i =b i ÷a i ′ (II) wherein a i ′ represents a fluorescent light source intensity correction image value for said each individual pixel of said uniform fluorescent plate, a i represents said intensity value of said each individual pixel of said uniform fluorescent plate, a Ave represents said average value of reference quantity of fluorescent light distribution image of said uniform fluorescent plate, c i represents said fluorescent light source intensity corrected sample image value for said each individual pixel of said biochip, and b i represents the intensity value of said each individual pixel of said measurement samples on said biochip, and wherein i represents a number of said each individual pixel of said uniform fluorescent plate and of said measurement samples of said biochip, and has a value of 1 to n.