Patent ID: 8637286

Claim:
A non-naturally occurring microbial organism comprising: (1) at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance carbon flux through acetyl-CoA, wherein said at least one exogenous nucleic acid encodes an enzyme selected from the group consisting of: i) an Acetyl-CoA synthase, ii) a Pyruvate ferredoxin oxidoreductase or pyruvate dehydrogenase, and iii) a pyruvate formate lyase; (2) (a) an isopropanol pathway that converts acetyl-CoA to isopropanol, wherein the isopropanol pathway comprises 1) an acetoacetyl-CoA thiolase; 2) an acetoacetyl-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA synthetase, or a phosphotransacetoacetylase/acetoacetate kinase; 3) an acetoacetate decarboxylase; and 4) an isopropanol dehydrogenase; (b) a 1,4-butanediol pathway that converts acetyl-CoA to 1,4-butanediol; wherein the 1,4-butanediol pathway comprises at least five enzymes selected from the group consisting of: 1) an acetoacetyl-CoA thiolase; 2) a 3-hydroxybutyryl-CoA dehydrogenase; 3) a crotonase; 4) a crotonyl-CoA hydratase; 5) a 4-hydroxybutyryl-CoA reductase (alcohol forming); 6) a 4-hydroxybutyryl-CoA reductase (aldehyde forming); 7) a 1,4-butanediol dehydrogenase; 8) a 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA synthetase, 4-hydroxybutyryl-CoA hydrolase, or phosphotrans-4-hydroxybutyrylase/4-Hydroxybutyrate kinase; and 9) a 4-hydroxybutyrate reductase; or (c) a 4-hydroxybutyrate pathway that converts acetyl-CoA to 4-hydroxybutyrate, wherein said 4-hydroxybutyrate pathway comprises at least five enzymes selected from the group consisting of: 1) an acetoacetyl-CoA thiolase; 2) a 3-hydroxybutyryl-CoA dehydrogenase; 3) a crotonase; 4) a crotonyl-CoA hydratase; 5) a 4-hydroxybutyryl-CoA transferase, hydrolase or synthetase; 6) a phosphotrans-4-hydroxybutyrylase; and 7) a 4-hydroxybutyrate kinase; and (3) a reductive TCA (rTCA) pathway, wherein said microbial organism comprises at least one exogenous nucleic acid encoding a rTCA pathway enzyme expressed in a sufficient amount to enhance carbon flux through acetyl-CoA, wherein said rTCA enzyme is selected from the group consisting of 1) an ATP-citrate lyase, 2) a citrate lyase, 3) a fumarate reductase, and 4) an alpha-ketoglutarate:ferredoxin oxidoreductase.