Patent ID: 7572949

Claim:
A method for determining whether a test compound is a candidate SKiNhead 1 (SKN-1)-mediated oxidative stress response-activating compound, comprising: (a) providing a first nematode capable of expressing a SKN-1 polypeptide and containing at least one reporter transgene comprising: (i) an oxidative stress resistance gene promoter, wherein the promoter comprises a SKN-1 binding site and is effective at directing SKN-1-induced gene expression, and (ii) a reporter gene operably linked to the promoter; (b) treating the first nematode with the test compound; and (c) determining whether expression of the reporter transgene is increased in the first treated nematode compared to a control nematode not treated with the test compound, wherein increased expression of the reporter transgene in the first treated nematode compared to the control nematode indicates that the test compound is a candidate SKN-1-mediated oxidative stress response-activating compound.