Patent ID: 7704703

Claim:
A method of identifying a compound that can induce the formation of a β-strand assembly of a Double Minute-2 (Dm2) polypeptide comprising: (a) contacting a compound with a Dm2 polypeptide selected from the group consisting of a polypeptide consisting of amino acid residues 235-259 of SEQ ID NO:8 and a polypeptide consisting of amino acid residues 275-289 of SEQ ID NO:8, wherein the Dm2 polypeptide can induce β-strand assembly with an Arf polypeptide (Alternative Reading Frame-p 19/Alternative Reading Frame-pI 4 polypeptide) set forth in SEQ ID NO: 4; and (b) determining in vitro whether the Dm2 polypeptide is induced to form a β-strand assembly; wherein a compound is identified when the Dm2 polypeptide is induced to form a β-strand assembly in the presence of the compound and not in the absence of the compound.