Patent ID: 7723081

Claim:
An Escherichia coli bacterium in which: (a) the intracellular activities of all of the following enzymes are enhanced: dihydrodipicolinate synthase, aspartokinase, dihydrodipicolinate reductase, phosphoenolpyruvate carboxylase, nicotinamide adenine dinucleotide transhydrogenase, and aspartate-semialdehyde dehydrogenase; and (b) the intracellular activities of at least one of the following enzymes are enhanced: i) diaminopimelate dehydrogenase, or ii) tetrahydrodipicolinate succinylase and succinyldiaminopimelate deacylase, wherein said activities are enhanced by: a) introducing a promoter or promoters which are operably linked to the gene encoding the enzymes into the chromosome of said bacterium, b) increasing the copy number of the genes encoding the enzymes by introducing one or more expression vectors into said bacterium, or c) combinations thereof.