Patent ID: 8592186

Claim:
A process for the preparation of chondroitin from a bacterial capsular polysaccharidic precursor comprising growing and amplifying a bacterium transformed with multiple copies of an expression cassette comprising a nucleotide sequence encoding an rfaH protein, said rfaH protein-coding sequence comprising the nucleotide sequence of SEQ ID NO: 9, SEQ ID NO: 13 or SEQ ID NO: 20, wherein said bacterium is Escherichia coli , selected from strains: K5, K4, and K30 having as polysaccharide precursor a K5, K4 and K30 capsular polysaccharide, respectively, or said bacterium is Pasteurella multocida ; and wherein said growing and amplifying step comprises a first batch phase, a second fed-batch phase and a microfiltration regimen; recovering a cell-free concentrated supernatant via ultrafiltration; hydrolyzing the cell-free concentrated solution; and recovering said chondroitin from said cell-free concentrated solution.