Patent ID: 8876734

Claim:
A method of establishing a fluid flow path between a patient and a fluid container, the method comprising: establishing fluid communication between the patient and a lumen-containing device; withdrawing a first volume of a bodily-fluid from the patient, the first volume of bodily-fluid being greater than the volume of the lumen-containing device and less than 5 ml; transferring the first volume of bodily-fluid to a fluid reservoir via the lumen-containing device; fluidically isolating the lumen-containing device from the fluid reservoir to sequester the first volume of bodily-fluid in the fluid reservoir; and establishing fluid communication between the patient and the fluid container with the lumen-containing device, whereby sequestering the first volume of bodily-fluid in the fluid reservoir sequesters microbes present in the first volume of bodily-fluid from the fluid flow path, thereby reducing contamination in the fluid flow path and any subsequent volumes of bodily-fluid withdrawn from the patient.