Patent ID: 7387874

Claim:
A method for determining an acquired predictive risk factor for a non-hematologic disease in a human without cancer having a premalignancy that is an adenoma, or non-hematopoietic dysplastic or hyperplastic cells or tissue, the method comprising the steps of: a. purifying extracellular nucleic acid from blood from a human without cancer to prepare extracted extracellular nucleic acid containing DNA encoding a mutated gene or fragment thereof; and concurrently or sequentially, b. enriching the extracted extracellular nucleic acid for the mutated gene DNA or a fragment thereof, wherein the mutated gene DNA or a fragment thereof is concentrated or isolated from the remaining extracted extracellular nucleic acid; c. amplifying the mutated gene DNA or a fragment thereof, or amplifying a signal from the enriched mutated DNA or a fragment thereof; and d. detecting the product of the amplified mutated gene DNA or the product of its amplified fragment, or the amplified signal of the mutated DNA or the amplified signal of a fragment thereof, whereby said detection determines a predictive risk factor for a nonhematologic disease in the human without cancer.