Patent ID: 7276357

Claim:
A composition for performing a polynucleotide replication reaction, which comprises a buffer, one or more template polynucleotides, nucleotide triphosphates, one or more polymerase enzymes or fragments thereof, and one or more reaction adjuvants comprising compounds of the formula: wherein: R 1 is C or S; and when R 1 is C, X is ═O, R 3 is N and R 6 is absent; when R 1 is S, X is ═O or and R 3 is C; R 2 is H or CH 3 only when one or more of R 4 , R 5 and R 6 is not H, and otherwise R 2 is an unsubstituted or halogen-, hydroxyl- or alkoxy- substituted alkyl or cycloalkyl of length m, wherein m is selected such that the total number of carbons in the compound is between 3 and 8 when R 1 is C and between 2 and 8 when R 1 is S; wherein any two of R 2 , R 3 , R 4 , R 5 and R 6 can form a cyclic structure in which cyclization is effected through a bond between them; R 4 , R 5 and R 6 each is H, alkyl, cycloalkyl, halogen-, hydroxyl or alkoxy- substituted alkyl or cycloalkyl of length n, wherein n is selected such that the total number of carbons in the compound is between 3 and 8 when R 1 is C and between 2 and 8 when R 1 is S; when R 4 and R 5 are CH 3 , R 2 cannot be H or CH 3 ; wherein the reaction adjuvant comprises a cyclic compound, wherein the cyclization is effected through a bond between any two of R 2 , R 3 , R 4 , R 5 , and R 6 ; wherein the cyclic portion of the compound comprises five, six or seven members; and wherein R 1 is C, R 3 is N, R 6 is absent and of R 4 or R 5 is H.