Patent ID: 7820377

Claim:
A method for HLA typing of HLA-A to generate subgroups A to O, by the unambiguous determination of short DNA sequence elements (2-6 bases) simultaneously on both parental alleles at a set of multiple selected positions in HLA-A gene, wherein the set of positions consists essentially of positions 98, 414, 539, 282, 571, 368, 256, 292, 238 and 270 (according to the numbering of the HLA-A gene starting at cDNA sequence position 1 of exon 1 as depicted in FIG. 1 ) comprising the steps for each position of: a) hybridizing a combination of oligonucleotides (primers) complementary to all known sequence variants to a DNA strand upstream of a given position; b) carrying out a primer extension reaction with at least one of the four dNTP substrates substituted by a terminating analog; c) analyzing the products by mass spectrometry which results in masses allowing unambiguous identification of the used primers and the added bases.