Patent ID: 8383880

Claim:
A genetically modified (GM) fish whose genome comprises a fusion transgene operably linked to a fish gonad-specific promoter, wherein the fusion transgene comprises: (a) a nitroreductase (NTR)-encoding gene, and (b) a reporter gene operably linked to the nitroreductase (NTR)-encoding gene, wherein the fish gonad-specific promoter is selected from the group consisting of a fish ovary-specific promoter and a fish testis-specific promoter, wherein a female of the GM fish expresses the fusion transgene that is operably linked to the ovary-specific promoter and a male of the GM fish expresses the fusion transgene that is operably linked to the testis-specific promoter, and further wherein treatment with a NTR-activated cytotoxic prodrug induces infertility of the female of the GM fish and increases frequency of infertility of a homozygous male of the GM fish as compared to that of a wild counterpart.