Patent ID: 7629158

Claim:
A composition comprising an amylase selected from the group consisting of: a.) an amylase having SEQ. ID NO: 5, said amylase having one of the following groups of mutations: (i) M15T+H133Y+N188S+A209V; (ii) M15T+H133Y+N188T+A209V; (iii) H133Y+N188S+G475R; or (iv) H133Y+N188S; b.) an amylase having SEQ. ID NO: 6, said amylase having one of the following groups of mutations: (i) M15T+R23K+H133Y+N188S+A209V; (ii) M15T+R23K+H133Y+N188T+A209V; (iii) R23K+H133Y+N188S+G475R; (iv) R23K+H133Y+N188S; (v) M15T+H133Y+N188S+A209V; (vi) M15T+H133Y+N188T+A209V; (vii) H133Y+N188S+G475R; or (viii) H133Y+N188S c.) and combinations thereof and a sufficient amount of calcium to provide a wash liquor comprising said composition with a free calcium concentration of from about 0.1 ppm to about 500 ppm.