Patent ID: 6946153

Claim:
A method of treating a patient suffering a disease associated with Trichophyton mentagrophytes or Pityrosporum ovale by applying topically an anti-fungal pharmaceutical composition comprising a therapeutically effective amount of a product prepared from rhizomes of Zingiber officinale , as an active ingredient, in admixture with a pharmaceutically acceptable carrier or diluent for the active ingredient, wherein said product is prepared by the following steps: a) preparing a crude liquid from rhizomes of Zingiber officinale; b) introducing the crude liquid to a reverse phase chromatography column, and eluting the column with water, a first eluent and a second eluent in sequence, said second eluent having a polarity weaker than that of the first eluent but stronger than that of chloroform, so that a first eluent resulting from elution of the first eluent and a second eluate resulting from elution of the second eluent are obtained; c) removing the first eluent from the first eluate by evaporation, so that a first concentrated eluate is obtained and is able to be used as the product; and d) removing the second eluent from the second eluate by evaporation, so that a second concentrated eluate is obtained and is able to used as the product; wherein step a) comprises steps i) to iv), or comprises step I), step I′), or step I″), wherein said steps i) to iv) are: i) shedding fresh rhizomes of Zingiber officinale and filtering the resulting mixture to obtain a filtrate and a residue; ii) extracting the filtrate with a first organic solvent, recovering the resulting extraction solution of the first organic solvent, and evaporating the first organic solvent from the extraction solution to obtain a first concentrated extraction solution; iii) extracting the residue with a second organic solvent, recovering the resulting extraction solution of the second organic solvent, and evaporating the second organic solvent from the extraction solution to obtain a second concentrated extraction solution; and iv) combining the first concentrated extraction solution and the second concentrated extraction solution to obtain the crude liquid; said step I) is: I) extracting powder of dried rhizomes of Zingiber officinale with the second organic solvent, recovering the resulting extraction solution of the second organic solvent, and evaporating the second organic solvent from the extraction solution to obtain the crude liquid; said step I′) is: I′) steam distilling powder of dried rhizomes of Zingiber officinale , and concentrating the resulting distillate by evaporation to obtain the crude liquid; and said step I″) is: I″) extracting powder of dried rhizomes of Zingiber officinale with supercritical CO 2 , recovering the resulting extraction solution of the supercritical CO 2 , and evaporating CO 2 from the extraction solution to obtain the crude liquid.