Patent ID: 7163800

Claim:
A method of screening a composition for non-receptor-specific G-protein coupled receptor (GPCR) desensitization inhibitory activity comprising the steps of: (a) providing a first cell comprising a first GPCR and a first conjugate of a marker molecule and a protein associated with the desensitization pathway of the first GPCR, the first GPCR being a GPCR that requires an agonist for desensitization or being a constitutively desensitized GPCR; (b) exposing the first cell to a test composition and, when the first GPCR requires agonist for desensitization, to an agonist for the first GPCR; (c) determining, through detection of the marker molecule in the first conjugate, whether or not the composition gives an indication of GPCR desensitization inhibitory activity with respect to the first GPCR; (d) providing a second cell comprising a second GPCR different from the first GPCR and a second conjugate of a marker molecule and a protein associated with the desensitization pathway of the second GPCR, the second conjugate being the same or different from the first conjugate, the second GPCR being a GPCR that requires an agonist for desensitization or being a constitutively desensitized GPCR; (e) exposing the second cell to the test composition and, when the second GPCR requires agonist for desensitization, to an agonist for the second GPCR; (f) determining, through detection of the marker molecule in the second conjugate, whether or not the composition gives an indication of GPCR desensitization inhibitory activity with respect to the second GPCR; wherein an indication of GPCR desensitization inhibitory activity for the test composition in both step (c) and step (f) being an indication that the test composition has non-receptor-specific GPCR desensitization inhibitory activity.