Patent ID: 7425543

Claim:
A method of encapsulating antisense oligonucleotides (oligomers) to NF-kB, comprising the steps of: a) dissolving albumin in water; b) solubilizing said antisense oligonucleotides (oligomers) to NF-kB in an aqueous phase selected from the group consisting of phosphate buffered saline (PBS), saline, water and water with Tween® 80; c) mixing said dissolved albumin and said solubilized antisense oligonucleotides (oligomers) to NF-kB together; d) cooling said mixture formed in step c); e) cooling a nonaqueous first solvent comprising either a lower carbon alcohol selected from the group consisting of methanol, ethanol, propanol, and butanol or an inert oil selected from the group consisting of olive oil, canola oil, cottonseed oil, mineral oil, mixtures of the foregoing oils and subcomponents of the foregoing oils; f) nebulizing the mixture of step d) into said cooled nonaqueous first solvent while said nonaqueous first solvent is stirred so as to form microspheres containing antisense oligonucleotides (oligomers) to NF-kB; g) crosslinking said microspheres with glutaraldehyde while stirring; h) washing said microspheres of step g) with a second solvent; i) sizing said microspheres of step h); and, j) freeze drying said microspheres of step i), whereby said antisense oligonucleotides (oligomers) to NF-kB are encapsulated in said microspheres.