Patent ID: 8110793

Claim:
Method for the acquisition of a fragment ion spectrum of biopolymer ions in a tandem mass spectrometer, comprising the following steps: a) acquiring a first fragment ion spectrum of a biopolymer ion using a fragmentation method with first fragmentation method parameters, b) calculating a quality coefficient in real time from the first fragmention ion spectrum data, wherein the quality coefficient corresponds to a length of a chain of polymer building blocks inferred from signals of the first fragment ion spectrum data, c) deciding from the value of the quality coefficient, whether a further fragment ion spectrum of this biopolymer ion is to be acquired, and d) in case of a positive decision, immediately reacquiring a second fragment ion spectrum of a biopolymerion of a same type as that used in step (a) using the same fragmentation method used in step (a) with second fragmentation method parameters that differ from the first fragmentation method parameters.