Patent ID: 8389298

Claim:
A method for detecting a labeled compound in a sample wherein the labeled compound comprises a compound linked to a chemiluminescent label, said labeled compound having the formula: wherein each of R 1 , R 2 and R 3 is selected from substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted aryl, substituted or unsubstituted aralkyl groups of 1-20 carbon atoms, wherein R 1 and R 2 can be joined together in a ring, R 4 -R 11 are independently selected from hydrogen and substituents which do not interfere with the generation of chemiluminescence, and at least one of the groups R 1 -R 11 is linked to the compound by means of a labeling substituent L-RG where L is a linking group and RG is a reactive group said method comprising: a) reacting the labeled compound with a peroxide and a transition metal compound selected from transition metal ions, transition metal salts, or transition metal complexes for producing chemiluminescence from the chemiluminescent label; and b) detecting the chemiluminescence as an indication of the presence of the compound.