Patent ID: 8703915

Claim:
A method for producing a mutant G-protein coupled receptor (GPCR) with increased stability relative to a parent GPCR, the method comprising changing one or more amino acids in the amino acid sequence that defines a parent GPCR, wherein (i) the one or more changed amino acids are located within a window of i plus or minus 5 residues, where i is the position of amino acid residue 2.46 in the parent GPCR when the parent GPCR is a Class 1 GPCR, or where i is the position of an equivalent amino acid residue in the parent GPCR when the parent GPCR is a Class 2 or 3 GPCR, or (ii) the one or more changed amino acids are located within an amino acid sequence of transmembrane helix 7 in the parent GPCR which amino acid sequence interacts with the window of i plus or minus 5 residues, to provide one or more mutants of the parent GPCR with increased stability.