Patent ID: 8728982

Claim:
An engineered recombinant nucleic acid phage hybrid vector for expressing phage displayed fusion peptides or proteins that bind to selected biologically active ligands, comprising a. a recombinant Pel B phage leader coding nucleic acid sequence; operably linked to: b. a recombinant tag, promoter, or selection coding nucleic acid sequence; operably linked to: c. a recombinant pIX encoding nucleic acid sequence; operably linked to: d. a recombinant restriction site; operably linked to: e. a peptide linker encoding nucleic acid sequence; operably linked to a: f. a first exogenous peptide or protein encoding sequence that selectively binds to a biologically active ligand, wherein said first exogenous peptide or protein encoding sequence is fused to said recombinant pIX encoding nucleic acid sequence; and g. a pVII encoding nucleic acid sequence; h. a native pIX encoding nucleic acid sequence; i. a pIII encoding nucleic acid sequence; and a pVI encoding nucleic acid sequence, wherein said vector encodes a second exogenous peptide or protein fused to a phage coat protein selected from pVII and pIII, wherein said phage hybrid vector expresses functional forms of said first exogenous peptide or protein and said second exogenous peptide or protein in a bacterial host cell system without the use of helper phage.