Patent ID: 8110677

Claim:
A process for obtaining purified Artemisia sphaerocephala polysaccharide with microwave-assistance, comprising: a) contacting Artemisia sphaerocephala seed powder with supercritical CO 2 to obtain an oil-extracted powder; contacting the oil-extracted powder with distilled water at a ratio of 1:30 to 1:50 (w/w) to obtain a suspension; exposing the suspension to microwaves of a frequency of 915 MHz and overall power of 600 W to 800 W in a microwave extraction apparatus comprising a water condenser, thereby obtaining an extractant; filtering the extractant at a pressure of 0.07 to 0.09 MPa to obtain a filtrate; subjecting the filtrate to a pressure of 0.06 to 0.08 MPa to reduce the total volume of the filtrate by ¾ to ⅞, thereby obtaining a concentrate; contacting the concentrate with ethanol to obtain a concentrated extract which comprises about 70 to 80% ethanol (v/v); centrifuging the concentrated extract at 3500 to 4000 rpm to obtain a precipitate; and subjecting the precipitate to a pressure of 1 Pa and a temperature of −60 to −50° C. for 24 to 36 hours to obtain material A; b) i) contacting material A with distilled water at a ratio of 1:150 to 1:200 (w/v) to obtain an aqueous solution; ii) heating the aqueous solution at a temperature of 60 to 70° C. and then incubating with 6μ-8μ/100ml papain for 10 to 12 hours; iii) contacting the aqueous solution with a preparation of butanol and chloroform wherein the butanol:chloroform ratio is 1:2 to 1:4 (v/v); iv) recovering the aqueous phase; v) repeating steps iii) and iv) a further 3 to 6 times to prepare a final aqueous phase; vi) subjecting the final aqueous phase to a pressure of 0.06 to 0.08 MPa and a temperature of 40 to 50° C. to collect and concentrate the aqueous phase to ⅛ to ¼ of original volume, thereby obtaining material B; and c) contacting material B with ammonia to obtain a solution of pH 9; contacting the solution of pH 9 with a preparation of 20 to 30% H 2 O 2 to obtain a colorless solution; subjecting the colorless solution to a pressure of 0.06 to 0.08 Mpa, thereby obtaining a concentrated solution; contacting one side of a semi-permeable membrane with the concentrated solution and another side of the semi-permeable membrane with distilled water, wherein the semi-permeable membrane has an average molecular weight cut-off of 8000 to 14000 Da, for 36 to 48 hours; adding ethanol to the dialyzed preparation to obtain an extract solution which comprises about 70 to 80% ethanol (v/v); centrifuging the extract solution at 3500 to 4000 rpm to collect a precipitate; and subjecting the collected precipitate to a pressure of 1 Pa and a temperature of −60 to −50° C. for 24 to 36 hours, thereby obtaining purified Artemisia sphaerocephala polysaccharide.