Patent ID: 7122354

Claim:
A cultured cell comprising a nucleic acid sequence that encodes the signal peptide of human growth hormone (hGH) linked to a nucleic acid encoding a polypeptide selected from the group consisting of α-1 antitrypsin, antithrombin III, apolipoprotein E, apolipoprotein A-1, Factor V, Factor VII, Factor VIII, Factor IX, Factor X, Factor XIII, bone growth factor-2, bone growth factor-7, calcitonin, a catalytic antibody, DNAse, erythropoietin, FSH-β, DNAase, globin, glucagon, granulocyte macrophage colony stimulating factor, an immune response modifier, an immunoglobulin, insulin, insulinotropin, an insulin-like growth factor, interferon-β, an interferon-β nerve growth factor, interleukin-1, interleukin-3, interleukin-4, interleukin-6, interleukin-11, interleukin-12, an interleukin-2 receptor, an IL-1 receptor antagonist, a low density lipoprotein receptor, macrophage-colony stimulating factor, parathyroid hormone, protein kinase C, soluble CD4, superoxide dismutase, tissue plasminogen activator, transforming growth factor β, tumor necrosis factor, thyroid stimulating hormone β, tyrosine hydroxylase, and urokinase, wherein the nucleic acid sequence is stably integrated into the cell and provides increased expression and secretion of the polypeptide from the cell as compared to expression and secretion of the polypeptide with a signal peptide that is naturally associated with the polypeptide.