Patent ID: 6929747

Claim:
A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode, said method comprising sequentially perfusing the system with a first solution comprising the biomolecule mixture, and a second solution comprising a displacer having a structure selected from formula I wherein Ar is phenyl, naphthalenyl, phenanthrenyl, anthracenyl, perylenyl, pyrenyl, fluorenyl, triazolyl, carbazolyl, indolyl, benzocarbazolyl, acridinyl, phenothiazinyl, or thianthrenyl; R 1 , R 2 , and R 3 are independently alkyl, aryl, heteroalkyl, alkylaryl, oxaalkyl, oxaaryl, oxaalkylaryl, substituted alkyl, substituted aryl, substituted alkylaryl, substituted oxaalkyl, substituted oxaaryl, substituted oxaalkylaryl or a direct bond; and at least one of R 1 , R 2 , and R 3 is other than a direct bond; X 1 , X 2 , and X 3 are independently hydrogen, X 5 or X 6 ; and at least one of X 1 , X 2 , and X 3 is other than hydrogen; X 5 is sulfonate, sulfate, phosphate, or carboxylate; X 6 is tertiary amino or quaternary amino; Y 1 , Y 2 , and Y 3 are independently O, S or a direct bond; and Z is CH or N.