Patent ID: 7001747

Claim:
An isolated recombinant nucleic acid molecule encoding a Type I polyketide synthase (PKS) wherein said nucleic acid molecule is produced by: a) providing a polynucleotide encoding at least an extension module of a PKS wherein said extension module contains at least one reductive domain; b) deleting a portion of the polynucleotide which corresponds to said at least one reductive domain of said PKS; and c) replacing said deleted portion with a polylinker region having multiple restriction enzyme sites; wherein said deleted portion corresponding to said at least one reductive domain is at least one domain selected from the group consisting of: a β-ketoreductase (KR) domain, a dehydratase (DH) domain and an enoyl reductase (ER) domain; wherein said polylinker is an in-frame addition to the PKS; and wherein said polylinker encodes a polypeptide.