Patent ID: 7649683

Claim:
Process for the observation of at least one sample region with a light raster microscope having an illumination axis, comprising the steps of: (a) generating illumination light for illuminating a sample, (b) moving the illumination light and the sample relative to each other along at least one scanning axis substantially perpendicular to the illumination axis, using a first scanner, to illuminate several sample points lying on a line, the relative movement of the illumination light and the sample defining a plane of relative movement, (c) simultaneously detecting the several illuminated sample points lying on a line, using a spatially resolving detector, (d) moving the illumination light using a second scanner scanning at an angle to the plane of the relative movement, (e) acquiring an image by coupling the movements of the first and second scanners and performing a three-dimensional sampling movement by illumination into an illumination region of the sample, and (f) calibrating at least the second scanner with the aid of a precalibration to at least one of the mass of the sample used and the mass of the objective used and the mass of the sample tray used for the generation of a control curve, and wherein step (d) is carried out with the aid of an acquired calibration curve.