Patent ID: 8512731

Claim:
A method of inhibiting growth of a microorganism on a surface of a medical device comprising identifying a susceptible surface on the medical device that is observed to be colonized in vivo by a microorganism when the device is implanted in an individual and coating the surface of the medical device with: (a) a first layer comprising an antimicrobial composition that includes an enzyme that generates hydrogen peroxide upon exposure to a ligand of the enzyme; and (b) a second layer disposed on the first layer comprising: an antimicrobial composition comprising a polyurea-silicone copolymer having a quaternary amine moiety, wherein: the first layer is disposed on the medical device so as to allow hydrogen peroxide generated by the enzyme in the first layer to diffuse away from the enzyme and contact a microorganism attempting to grow on the medical device thereby inhibiting its growth; the second layer is formed from a reaction mixture comprising a diisocyanate, a hydrophilic polymer which is selected from the group consisting of a hydrophilic polymer diol, a hydrophilic polymer diamine and combinations thereof, and a siloxane; the quaternary amine moiety in the second layer inhibits the growth of a microorganism that contacts the second layer, so that microbial growth is inhibited on the surface of the medical device when the surface is exposed to a microorganism, and the device is a glucose sensor comprising a plurality of layers, wherein the plurality of layers include: an electrode having an electrochemically reactive surface area; an analyte sensing layer that detectably alters electrical current at the electrode in the presence of an analyte; and an analyte modulating layer that modulates diffusion of an analyte therethrough.