Patent ID: 7060506

Claim:
A method for monitoring activity of one or more enzymes comprising the steps of: A. mixing: (i) one or more tagged binding partner polypeptides; (ii) one or more binding partner polypeptides that bind to said one or more tagged binding partner polypeptides of (i); and (iii) one or more enzymes that add or remove a moiety to or from said one or more binding partner polypeptides or one or more tagged binding partner polypeptides; wherein said one or more binding partner polypeptides are immobilized on a solid support; and wherein said one or more tagged binding partner polypeptides or said one or more binding partner polypeptides comprise one or more sites for the addition or removal of said moiety, wherein addition or removal of said moiety promotes binding of said one or more binding partner polypeptides with the corresponding one or more tagged binding partner polypeptides; under conditions which promote binding of said one or more binding partner polypeptides with said one or more tagged binding partners; and B. detecting binding of said one or more tagged binding partner polypeptides of (i) to said one or more binding partner polypeptides of (ii), wherein the step of detecting binding comprises adding one or more detector molecules comprising a first region that associates with a tag of said one or more tagged binding partner polypeptides and a second region comprising one or more reporter molecules, wherein detection of binding as a result of said mixing is indicative of enzyme activity.