Patent ID: 6897045

Claim:
A composition comprising: a first adeno-associated virus vector comprising linked: i) a first nucleic acid segment comprising a 5′-inverted terminal repeat of adeno-associated virus; ii) a second nucleic acid segment comprising a portion of a gene which includes a transcriptional regulatory region; iii) a third nucleic acid segment comprising a splice donor site; and iv) a fourth nucleic acid segment comprising a 3′-inverted terminal repeat of adeno-associated virus; and b) a second adeno-associated virus vector comprising linked: i) a first nucleic acid segment comprising a 5′-inverted terminal repeat of adeno-associated virus; ii) a second nucleic acid segment comprising a splice acceptor site; iii) a third nucleic acid segment comprising a portion of a gene which together with the nucleic acid segment of a)(ii) comprises a gene comprising an open reading frame which encodes a functional polypeptide; and iv) a fourth nucleic acid segment comprising a 3′-inverted terminal repeat of adeno-associated virus.