Patent ID: 7771981

Claim:
A purified nucleic acid segment comprising a coding region encoding enzymatically active heparin synthase, wherein the heparin synthase is a single protein that is a dual-action catalyst that utilizes UDP-GlcUA and UDP-GlcNAc to synthesize heparin, and wherein the purified nucleic acid segment is at least one of: (a) a purified nucleic acid segment encoding the Pasteurella multocida heparin synthase of SEQ ID NO:6; (b) the purified nucleic acid segment of SEQ ID NO:5; (c) a purified nucleic acid segment that is at least 90% identical to SEQ ID NO:5; and (d) a purified nucleic acid segment that hybridizes to a sequence, which is fully complementary to SEQ ID NO:6 under hybridization conditions of 5× Standard Saline Citrate (SSC) at 50° C. overnight, followed by washing in 0.1×SSC at room temperature for 10-60 minutes.