Patent ID: 7867454

Claim:
A microfluidic device comprising: a first layer and a second layer; a plurality of reaction cells, each reaction cell comprising a first chamber and a second chamber, said first chamber and said second chamber being in fluid communication through an interface channel having an interface valve associated therewith for controlling fluid communication between said first chamber and said second chamber wherein said chambers and said interface channel are present in the second layer; wherein each said first chamber is in fluid communication with a first fluid channel located in said second layer, and each said first fluid channel is in fluid communication with a plurality of first chambers of a plurality of reaction cells; wherein each said second chamber is in fluid communication with a second fluid channel in the second layer, said second fluid channel is in fluid communication with a third fluid channel; and a plurality of said third fluid channels are in fluid communication with a fourth fluid channel, wherein said fourth fluid channel is located in said first layer.