Patent ID: 7803551

Claim:
A method of PCR analysis comprising the steps of: providing a mixture of a dsDNA binding dye, at least one target nucleic acid, and primers configured for amplifying the target nucleic acid, amplifying the target nucleic acid in the presence of the dsDNA binding dye, monitoring fluorescence of the dsDNA binding dye, generating a melting curve for the target nucleic acid, normalizing magnitude differences of the melting curve, repeating the providing, amplifying, generating and normalizing steps with at least one additional target nucleic acid, and plotting a fluorescence difference between the magnitude-difference-normalized melting curves, wherein the magnitude-difference-normalized melting curve of one target nucleic acid is selected as a standard and is plotted as the standard across melting temperatures and the magnitude-difference-normalized melting curve for each additional target nucleic acid is plotted as a difference from the standard across the melting temperatures.