Patent ID: 7759058

Claim:
A method to prepare a perfuming composition or a perfumed article which has anti-microbial activity against at least 2 different kinds of bacteria selected from the group consisting of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus hirae , which comprises: (i) testing a perfuming ingredient to determine if it has the desired anti-microbial activity by: solubilizing the perfuming ingredient in an aqueous medium at a concentration between 250 and 1000 μg/ml, relative to the aqueous medium, in the presence of an effective amount of a testing solvent which is substantially non-toxic for the different types of bacteria that are to be tested and which allows complete solubilization of said perfuming ingredient; adding an inoculum of the different types of bacteria that are to be tested such that the concentration of the bacteria in the aqueous medium is at least 10 7 colony forming units/ml; diluting the aqueous medium so as to reduce the concentration of the bacteria therein to 10 3 colony forming units/ml; spreading a volume of a diluted aqueous medium of 10 2 colony forming units of the bacteria onto a culture medium; counting the surviving colonies after incubation; comparing the number of surviving colonies obtained with that of a control that does not contain said perfuming ingredient; and selecting only those perfuming ingredients which have 100% effectiveness against at least 2 different kinds of bacteria to form the anti-microbial perfuming composition or perfumed article; and (ii) preparing the perfuming composition or perfumed article by admixing with other perfuming ingredient(s), solvent(s) and adjuvant(s) of current use in perfumery at least 30% by weight of one or more of the selected perfuming ingredients that have the 100% effectiveness against the at least 2 different kinds of bacteria.