Patent ID: 8206966

Claim:
An alpha-amylase variant comprising an amino acid sequence at least 95% identical to a wild-type alpha-amylase polypeptide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 15, and SEQ ID NO: 16, and having a substitution at an amino acid position corresponding to position 242 of SEQ ID NO: 1, wherein said variant has alpha-amylase activity and a T m about 5-10° C. higher than said wild-type alpha-amylase; and wherein the substitution at an amino acid position corresponding to position 242 of SEQ ID NO: 1 is selected from the group consisting of S242A, S242E, and S242Q.