Patent ID: 8822191

Claim:
A method of refolding an insoluble, recombinant, eukaryotic α(2,3)sialyltransferase (ST3Gal3) protein, wherein the ST3Gal3 protein comprises a maltose binding protein domain (MBD), the method comprising the steps of (a) solubilizing the insoluble, recombinant, eukaryotic ST3Gal3 protein in a solubilization buffer; and (b) contacting the soluble eukaryotic ST3Gl13 protein with a refolding buffer comprising a redox couple and polyethylene glycol) (PEG) and/or lauryl maltoside to refold the eukaryotic ST3Gal3 protein, wherein the refolded eukaryotic ST3Gal3 protein catalyzes the transfer of a sialic acid sugar from a donor substrate to an acceptor substrate.