Patent ID: 7785833

Claim:
A method for producing a fraction enriched in a proteinaceous molecule having N-linked glycans comprising sialyl-Lewis X, Lewis X or LacdiNac structures, the method comprising the steps of: (a) expressing recombinant proteinaceous molecules having N-linked glycans comprising sialyl-Lewis X, Lewis X or LacdiNac structures in a cell that expresses an adenoviral E1A protein; (b) fractionating the expressed recombinant proteinaceous molecules; and (c) isolating a fraction of recombinant proteinaceous molecules having a higher content of sialyl-Lewis X, Lewis X or LacdiNac structures in their N-glycans per proteinaceous molecule, as compared to the content of sialyl-Lewis X, Lewis X or LacdiNac structures per proteinaceous molecule in the recombinant proteinaceous molecules expressed in step (a).