Patent ID: 8329197

Claim:
An ex vivo method for eliciting a synergistic immunostimulatory effect on human immune cells comprising contacting human immune cells ex vivo with (i) at least one Toll-Like Receptor (TLR) agonist which is selected from the group consisting of TLR 1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8 and TLR9 agonists; and (ii) at least one CD40 agonist, wherein (i) and (ii) are each comprised in an amount such that, in combination with the other, are effective to produce a synergistic increase in the generation of activated CD8 + T cells or the generation of memory CD8 + T cells in response to an antigen by said immune cells, and transplanting said human immune cells in a subject in need thereof.