Patent ID: 7214785

Claim:
A single-chain Fv (scFv) polynucleotide comprising a sequence coding for a VH chain of a human antibody against blood coagulation factor VIII (FVIII) said human antibody against FVIII having an activity to inhibit the coagulation activity of human FVIII, linked to a sequence coding for a VL chain of the human antibody against FVIII, wherein said human antibody against FVIII specifically binds FVIII when FVIII is not bound to or in a complex with von Willebrand Factor (vWF), and wherein complementarity determining regions (CDR1 to CDR3) of said VH chain consist of the following amino acid sequences: CDR1: Glu Leu Ser Ile His (SEQ ID NO: 13); CDR2: Gly Leu Asp Arg Glu Asp Gly Lys Xaa Val Ser Ala Gln Mg Phe Gln Gly (Xaa=Thr or Ala) (SEQ ID NO: 14); and CDR3: Gly Val Ala Ser Asp Asp Asp Ala Phe Glu Ile (SEQ ID NO: 15), and wherein complementarity determining regions (CDR1 to CDR3) of said VL chain consist of the following amino acid sequences: CDR1: Arg Ala Ser Gln Ser Ile Xa1 Xa2 Tyr Leu Asn (Xa1=Ser or Thr: Xa2=Ser or Arg) (SEQ ID NO: 16); CDR2: Ala Ala Ser Ser Leu Gln Ser (SEQ ID NO: 17); and CDR3: Gln Xa1 Ser Tyr Xa2 Thr Pro Xa3 Thr (Xa1=Gln or His; Xa2=Ser or Thr; Xa3=Leu or Ile) (SEQ ID NO: 18).