Patent ID: 8133705

Claim:
A method of producing an optically active amino compound, the method comprising: reacting a carbonyl compound with an amino donor in the presence of a culture product, the culture product being a culture product of Pseudomonas fluorescens KNK08-18 (FERM BP-10599), or a culture product of a transformant obtained by transformation of a host cell with a vector having DNA, wherein the DNA (A) has the base sequence of SEQ ID NO: 2; or (B) is hybridizable with DNA complementary with the base sequence of SEQ ID NO: 2 under stringent conditions, and encodes an enzyme having an amino group transfer activity, which produces acetophenone and 1-benzyl-3-aminopyrrolidine from 1-benzyl-3-pyrrolidinone and optically active (S)-α-phenethylamine, wherein said stringent conditions are such conditions that hybridization is carried out at 65° C. in the presence of NaCl of 0.7M to 1.0M and washed at 65° C. with a 2×SSC solution, the culture product of Pseudomonas fluorescens KNK08-18 (FERM BP-10599) being (i) a culture broth containing Pseudomonas fluorescens KNK08-18 (FERM BP-10599), (ii) incubated Pseudomonas fluorescens KNK08-18 (FERM BP-10599), or (iii) a material prepared from the culture broth containing Pseudomonas fluorescens KNK08-18 (FERM BP-10599) or the incubated Pseudomonas fluorescens KNK08-18 (FERM BP-10599), the culture product of a transformant being (iv) a culture broth containing the transformant, (v) an incubated transformant, or (vi) a material prepared from the culture broth containing the transformant or the incubated transformant, the carbonyl compound being represented by General Formula (1): where P and Q are an substituted or unsubstituted alkyl group, branched alkyl group, aryl group, heteroaryl group, aryloxy group, heteroaryloxy group, alkoxy group, alkoxycarbonyl group, alkenyl group, alkynyl group, cycloalkyl group, aralkyl group, or heteroaralkyl group, and may form a ring by bonding with each other, but are different from each other in chirality, the optically active amino compound being represented by General Formula (2): where P and Q are the same as in General Formula (1).