Patent ID: 7732578

Claim:
A humanized antibody or a binding fragment thereof comprising: a humanized heavy chain variable region containing three CDR sequences from a heavy chain variable region of a subject non-human antibody grafted into a variable region framework of a candidate human germline heavy chain variable region sequence and comprises two human CDR sequences with the same canonical structure type as the non-human CDR sequences in the subject non-human antibody for two corresponding CDR positions, provided that residues 61-65 of CDR2 of the humanized heavy chain variable region are residues from positions 61-65 from the candidate human germline heavy chain variable region sequence; and a humanized light chain variable region containing three CDR sequences from a light chain variable region of the subject antibody grafted into a variable region framework of a candidate human germline light chain variable region sequence and comprises three human CDR sequences with the same canonical structure type as non-human CDR sequences from the subject non-human antibody for three corresponding CDR positions; wherein the humanized antibody or a binding fragment thereof selected from a Fab fragment, a (Fab)′2 molecule, and a single chain Fv molecule binds to the same antigen as the subject non-human antibody wherein the CDR boundaries and numbering of residues of the humanized antibody are as defined by Kabat.