Patent ID: 7091029

Claim:
A method for producing high titer recombinant Adeno-Associated Virus (rAAV) comprising: (a) simultaneously co-infecting a 293 cell with (i) a first replication defective recombinant herpes simplex virus (rHSV) comprising a nucleic acid encoding an AAV rep and an AAV cap gene each operably linked to a promoter; and (ii) a second replication defective rHSV comprising rAAV internal terminal repeat (ITR) nucleic acid segments encoding rAAV ITRs, a gene of interest, and a promoter operably linked to said gene of interest; (b) incubating the 293 cell; and (c) following incubation, collecting rAAV from the 293 cell of step (b) wherein the titer is up to 2160–4200 infectious particles per cell.