Patent ID: 7078387

Claim:
A method for stable and efficient transformation of cardiomyocytes which comprises: infusing a recombinant adeno-associated virus (AAV) vector into a coronary artery or a coronary sinus of an animal in an amount of about 1×10 5 to about 1×10 9 infectious units (IU) AAV per gram body weight and for a time sufficient to stably and efficiently transduce cardiomyocytes perfused through said artery or said sinus, wherein said AAV vector comprises at least one nucleic acid molecule operably linked to a control region, said nucleic acid molecule encoding an angiogenic protein, wherein at least 10% of the cardiomyocytes are transduced with the AAV and the AAV is present in the transduced cardiomyocytes for at least 4 weeks.