Patent ID: 6858852

Claim:
A method for operation of an image-generating optical system for detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior and, in particular, the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-active light emission and/or enzyme-active fluorescence, for the operation of a laser scanning microscope, comprising the steps of: splitting the image point information of the specimen into spectral components in a spatially resolved manner on the detection side in dependence on wavelength; and carrying out at least one summing for different spectral components, wherein a change in at least one irradiation wavelength and/or irradiation intensity is carried out within a scanning process between different specimen regions and a summing of at least some of the respective spectral components is carried out for different specimen regions and/or irradiation wavelengths/intensities; displaying the summed regions as images.