Patent ID: 8246966

Claim:
A composition comprising: as one component an isolated cell free preparation of protozoan microsomes; the cell free preparation of protozoan microsomes the resuspended pellet of a low speed spin of about 5,000 g to about 20,000 g of a lysate of a protozoan of the genus Trypanosoma selected from the group consisting of T. cruzi, T. brucei, T.b. gambiense , and T.b. rhodesiense ; and translocates a polypeptide into the microsome; and as a second component an isolated purified cell free preparation of protozoan cytosol; the preparation of protozoan cytosol the supernatant of a high speed spin of greater than 20,000 g of a lysate of a protozoan of the genus Trypanosoma selected from the group consisting of T. cruzi, T. brucei, T.b. gambiense , and T.b. rhodesiense ; and facilitates the translocation of a plasma membrane polypeptide into a cell free preparation of protozoan microsomes.