Patent ID: 7335381

Claim:
A composition for use as a bone implant comprising particulate bone including particles having sizes less than or equal to about 355 μm and having a particle size distribution including from about 24.6 wt % to about 36.3 wt % of particles having a particle size between about 350 μm and about 250 μm, from about 22 wt % to about 25 wt % of particles having a particle size between 250 μm and about 150 μm, and from about 36.7 wt % to about 46.7 wt % of particles having a particle size less than 150 μm, and prepared from bone having an initial temperature between about 18° C. and about 20° C. and ground in a mill under conditions so that the bone is not heated above a critical temperature of less than or equal to 40° C., where the particulate bone is non-chemically extracted, non-demineralized, and where said composition has improved osteoinductive activity and regeneration of bone defects as compared to demineralized particulate bone.