Patent ID: 8617852

Claim:
A method for producing an L-amino acid utilizing a microorganism, which comprises: A) culturing the microorganism in a medium, and B) collecting the L-amino acid from the medium, wherein the microorganism has been modified so that: a) it comprises isomaltase EC 3.2.1.122 activity, or b) the isomaltase EC 3.2.1.122 activity is increased by a method selected from the group consisting of: i) increasing the copy number of an isomaltase gene, ii) replacing the promoter for an isomaltase gene with a stronger promoter, and iii) combinations thereof; and wherein said isomaltase EC 3.2.1.122 activity causes the decomposition of phosphorylated isomaltose into glucose and glucose phosphate, wherein the isomaltase gene encodes a protein comprising the amino acid sequence shown in SEQ ID NO: 40, wherein the microorganism comprises a gene coding for PTS maltose enzyme IICB comprising the amino acid sequence shown in SEQ ID NO: 42, wherein said enzyme allows the microorganism to take up isomaltose; wherein the medium contains glucose and isomaltose as a carbon source, wherein the microorganism is an Escherichia coli, wherein the L-amino acid is L-lysine, L-threonine, or both.