Patent ID: 7585645

Claim:
A method for the preparation of an emulsion formulation, comprising: (a) introducing into a plant cell a chimeric nucleic acid sequence comprising (1) a first regulatory nucleic acid sequence, that regulates transcription in said cell, operatively linked to (2) a coding nucleic acid sequence that codes for a recombinant fusion polypeptide, said coding nucleic sequence comprising (i) a first nucleic acid sequence, encoding at least the central domain of an oil body protein to provide targeting to an oil body, linked in reading frame to (ii) a second nucleic acid sequence, encoding a thioredoxin or thioredoxin reductase, operatively linked to (3) a second regulatory nucleic acid sequence that regulates termination of transcription in said plant cell, such that said recombinant fusion polypeptide comprises an oil body protein and thioredoxin or thioredoxin reductase; wherein said plant cell is selected from the group consisting of an almond cell, an anise cell, an avocado cell, a beach nut cell, a borage cell, a Brazil nut cell, a candle nut cell, a cashew nut cell, a castor cell, a coconut cell, a coriander cell, a cotton cell, a crambe cell, a croton cell, a Cuphea cell, a Euphorbia cell, a Dimorphoteca cell, a false flax cell, a fennel cell, a groundnut cell, a hazelnut cell, a hemp cell, a honesty plant cell, a jojoba cell, a kapok fruit cell, a kukui nut cell, a Lesquerella cell, a linseed cell, a macademia nut cell, a maize cell, a meadow foam cell, a mustard cell, a oil palm cell, a oiticia cell, a paw paw cell, a pecan cell, a perilla cell, a physic nut cell, pilinut cell, a pine nut cell, a pistachio cell, a poppy seed cell, a rapeseed cell, a safflower cell, a sesame seed cell, a soybean cell, a squash cell, a sal tree cell, a Stokes a aster cell, a sunflower cell, a tukuma cell, a tung nut cell, and a veronia cell; (b) growing said plant cell to express said recombinant fusion polypeptide in a progeny plant cell comprising oil bodies; (c) isolating said oil bodies from said progeny plant cell expressing said recombinant fusion polypeptide; (d) washing said oil bodies to obtain a washed oil body preparation comprised of substantially intact oil bodies that comprise said recombinant fusion polypeptide; and (e) formulating said washed oil body preparation comprising substantially intact oil bodies into an emulsion.