Patent ID: 7875763

Claim:
A method of obtaining a plant seed with an increased fructan level comprising: a) obtaining a plant comprising: i) a first recombinant DNA construct comprising a seed-specific promoter operably linked to a nucleotide sequence encoding a polypeptide having sucrose:sucrose 1-fructosyltransferase activity, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:14; and ii) a second recombinant DNA construct comprising a seed-specific promoter operably linked to a nucleotide sequence encoding a polypeptide having fructan:fructan 1-fructosyltransferase activity, wherein the polypeptide has an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO:4; and b) obtaining seed from the plant of (a), wherein the seed comprises the first recombinant DNA construct and the second recombinant DNA construct, and further wherein the fructan level in the seed is increased when compared to fructan level in a seed that does not comprise the first recombinant DNA construct and the second recombinant DNA construct.