Patent ID: 8753864

Claim:
A recombinantly produced first organism, which is an E.coli. cell, into which is introduced nucleic acids encoding: a) a glycosyltransferase derived from a second organism which is a bacterial cell, wherein said glycosyltransferase assembling an oligosaccharide on a lipid carrier, b) a recombinant target protein derived from a third organism which is a bacterium, wherein the target protein comprises one or more inserted N-glycosylation optimized amino acid consensus sequence D/E-X-N-Z-S/T, wherein X and Z are independently selected from any natural amino acid except Pro, and wherein a nucleic acid sequence encoding said consensus sequence(s) is inserted through genetic engineering into the nucleic acid encoding said target protein in order to achieve an increased or optimized efficiency of the N-glycosylation of said target protein in the E.coli. cell compared to the same protein which does not comprise said N-glycosylation optimized amino acid consensus sequence; and c) an oligosaccharyl transferase which is derived from a fourth organism which is a bacterium; wherein said consensus sequence is N-glycosylated with said oligosaccharide by said oligosaccharyl transferase, wherein said second, third, and fourth organisms are different organisms.