Patent ID: 6962805

Claim:
A method of producing L-glutamic acid, comprising: culturing a coryneform bacterium expressing an enzyme encoded by a coryneform bacteria glutamic acid biosynthesizing gene, in a medium for a time and under conditions suitable to produce and accumulate said L-glutamic acid in the medium, and collecting said L-glutamic acid from the medium; wherein the glutamic acid biosynthesizing gene is located on a chromosome of the coryneform bacterium and the enzyme is selected from the group consisting of glutamate dehydrogenase, citrate synthase, isocitrate dehydrogenase, pyruvate dehydrogenase, and aconitase, said glutamic acid biosynthesizing gene comprising a DNA sequence situated at about position −35 from the transcription start site of the glutamic acid biosynthesizing gene, wherein said DNA sequence is selected from the group consisting of TTGTCA and TTGCCA, wherein said producing L-glutamic acid is at a level greater than the production of L-glutamic acid by the corresponding wild-type coryneform bacterium.