Patent ID: 8666674

Claim:
A method of preparing alloreactive cytotoxic T cells comprising: providing patient cell information, wherein the patient cell information comprises patient cell antigen information that is at least partly determined through one or more of serotyping or a high or intermediate resolution molecular sequencing method of major histocompatibility complex (MHC) information; generating stimulator information from donor cell antigen information, wherein the donor cell antigen information is determined at least partly through one or more of serotyping or a high or intermediate resolution molecular sequencing method of MHC information; generating responder information from the patient cell antigen information; comparing the responder information to stimulator information derived from the donor cell antigen information; identifying presence or absence of a partial mismatch between the stimulator information and the responder information among patient cell and donor cell pairs; selecting a patient cell and donor cell pair based on the presence of the partial mismatch, wherein the partial mismatch is predictive of an enhanced immune response in the patient; and inducing alloreactive cytotoxic T cells endogenous to the patient under conditions sufficient to induce the alloreactive cytotoxic T cells, wherein immune cells endogenous to the patient respond to stimulator antigen derived from the donor to induce the alloreactive cytotoxic T cells based on the patient cell and donor cell pair.