Patent ID: 8460910

Claim:
An altered 9° N DNA polymerase, comprising the sequence of SEQ ID NO: 14, wherein the first amino acid of the motif A region is mutated to an amino acid selected from the group consisting of the aromatic amino acids, the amino acids with aliphatic side chains, glutamine (Q), cysteine (C), and serine (S); the second amino acid of the motif A region is mutated to an amino acid selected from the group consisting of alanine (A), serine (S), and glycine (G); and the third amino acid of the motif A region is mutated to an amino acid selected from the group consisting of serine (S), alanine (A), glycine (G), and the amino acids having beta-branched side chains, whereby the altered 9° N DNA polymerase is capable of incorporating a 3′ O-azidomethyl-modified nucleotide.