Patent ID: 8859282

Claim:
A method for preservation of human hematopoietic stem/progenitor cells, wherein the said method comprising the step of: a) providing a plurality of purified lectins selected from the group consisting of garlic lectin (GL), Mus a paradisiacal lectin (BL) and Artocarpus integrifolia lectin (AL) by dialyzing against phosphate buffered saline followed by filtration through 0.2 to 0.5 micron filter followed by protein determination using a micro bicinchonic assay (BCA) kit to check protein loss; b) isolating a plurality of CD34 + cells from a human cord blood sample; c) incubating of the isolated CD34 + cells obtained from step (b) with different concentration of the purified lectins in ranges between 100 pg/ml to 10 ng/ml for a period of 10 to 30 days followed by a toxicity test; d) subjecting incubated CD34 + cells obtained from step (c) to a CFU assay or seeding on an irradiating M210B4 feeder for LTC-IC assays; and e) interpreting the effect of the purified lectins on human hematopoietic stem/progenitor cells preservation.