Patent ID: 8895710

Claim:
A method of separating one or more antibodies from one or more other compounds in a liquid sample, wherein a mobile phase comprising said antibodies and compound(s) is contacted with a separation matrix, wherein said separation matrix comprises a chromatography ligand defined by the following formula: R 1 —R 2 —N(R 3 )—R 4 —R 5 wherein R 1 is a non-substituted phenyl group; R 2 is —CH 2 —; R 3 is —CH 3 ; R 4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R 5 is OH, and wherein said chromatography ligand is immobilized to a support via the amine nitrogen atom, wherein said compounds comprise host cell proteins, protein A, or antibody aggregates and wherein the host cell proteins, protein A, or antibody aggregates are separated from the antibodies.