Patent ID: 7033773

Claim:
A method for determining whether a compound of interest is an agonist of a receptor which couples to both Gs and Gq proteins thereby affecting an adenylyl cyclase or phospholipase C pathway said method comprising: (a) introducing into a first and second cell line, an expression vector comprising a nucleotide sequence encoding said receptor which is not normally expressed in said first and second cell lines, wherein said first and second cell lines express urokinase-type plasminogen activator (u-PA) and said second cell line also has inhibited Gs signaling of u-PA activity relative to said first cell line; (b) contacting said first and second cell lines with said compound of interest; (c) measuring the u-PA activity by fluorescence or absorbance spectroscopy of the cell culture supernatant of step (b) and the cell culture supernatant of said first and second cell lines which have not been in contact with said compound of interest; and (d) determining whether said compound of interest is an agonist of a receptor which couples to both Gs and Gq proteins thereby affecting an adenylyl cyclase or phospholipase C pathway, wherein the u-PA activity of the supernatant from said first and second cell lines from step (b) is greater than the u-PA activity of the supernatant from said first and second cell lines which have not been in contact with said compound of interest.