Patent ID: 7807177

Claim:
An isolated or purified segment of hepatocyte growth factor (HGF) not having any sugar chains at glycosylation sites, (A) wherein said segment is a protein having a homology of at least 80% or higher with amino acids from positions 32 to 478 of SEQ ID NO: 1, (B) wherein said segment, (i) lacks sugar chains at glycosylation sites of an HGF α-chain, (ii) has an N terminal hairpin domain and four kringle domains, (iii) has an antagonist activity against the action of HGF via a c-Met/HGF receptor, and (iv) has a neovascularization inhibitory activity, (C) wherein the amino acids of the protein are substituted so as to prevent glycosylation at the glycosylation sites of the protein, and (D) wherein the substitutions of amino acids are the following (a), (b) and (c): (a) amino acid(s) at position(s) 294 and/or 296 of SEQ ID NO: 1 is/are substituted by another amino acid, and/or an amino acid at position 295 thereof is substituted by Pro, leading thereby to no glycosylation at position 294, (b) amino acid(s) at position(s) 402 and/or 404 of SEQ ID NO: 1 is/are substituted by another amino acid, and/or an amino acid at position 403 thereof is substituted by Pro, leading thereby to no glycosylation at position 402, and (c) an amino acid at position 476 of SEQ ID NO: 1 is substituted by another amino acid, leading thereby to no glycosylation at position 476.