Patent ID: 8822198

Claim:
A method for cultivating eukaryotic cells comprising the following steps: a) preparing a eukaryotic host cell which contains a recombinant gene of interest and produces a corresponding product of interest, b) cultivating the cell from step a) in a batch-fed fermentation process and in a cell culture medium which contains no HCO 3 − or CO 3 2− ions, said cell culture medium containing sodium-β-glycerophosphate-pentahydrate (C 3 H 7 Na 2 O 6 P×5H 2 O, Sod-β) in an amount effective to act as a buffering agent; and c) regulating the pH of the culture medium by means of a non-CO 2 -forming acid and/or alkaline solution, while the pCO 2 of the culture medium is regulated by the introduction of CO 2 , O 2 , N 2 or air.