Patent ID: 8389236

Claim:
A translational recoding reporter construct comprising: a) a first fluorescent protein coding sequence; b) a second fluorescent protein coding sequence encoding a fluorescent protein different from the first fluorescent protein; c) a linker sequence interposed between the first and second fluorescent protein coding sequences; and d) a recoding signal sequence interposed between the first fluorescent protein coding sequence and the second fluorescent protein coding sequence, wherein said recoding signal sequence comprises the sequence of SEQ ID NO:8; wherein the first fluorescent protein coding sequence and the second fluorescent protein coding sequence are out-of-frame with respect to each other but are able to be co-expressed as a single fusion polypeptide upon recoding, and wherein the linker sequence encodes a polypeptide capable of physically separating the co-expressed fluorescent proteins, and wherein said linker sequence encodes a polypeptide comprising the sequence of SEQ ID NO:1, resulting in increased fluorescence output from each protein.