Patent ID: 7771975

Claim:
A process for the analysis of a nucleic acid sample so as to determine the simultaneous presence in the sample of two or more target sequences each bounded by first and second oligonucleotide primer sequences, said process comprising the steps of: (i) providing a first multiplicity of different oligonucleotide primers, each covalently anchored at its 5′-terminus to a discrete, individually identifiable, solid support surface, wherein the first multiplicity comprises the first and second oligonucleotide primer sequences, (ii) providing a second multiplicity of oligonucleotide primers, in solution, wherein the nucleotide sequences of respective members of said first and second oligonucleotide primer multiplicities are identical to each other, (iii) performing a polymerase chain reaction amplification on said sample in the presence of both said first and second oligonucleotide primer multiplicities, so as to convert said anchored oligonucleotide primers to anchored polynucleotide chain extension products, (iv) separating said solid support surface anchored polynucleotide chain extension products from the resulting solution, and (v) determining, for each said separated solid support-linked chain extension product, which of said second multiplicity of oligonucleotide primers is a complementary sequence to a portion of a said chain extension product, whereby the presence of the two or more target sequences is detected in the sample.