Patent ID: 6893839

Claim:
A method for producing a secreted heterologous polypeptide, comprising: (a) cultivating a mutant cell of a parent Fusarium venenatum cell under conditions conducive for the production of the secreted heterologous polypeptide, wherein (i) the mutant cell comprises a first nucleic acid encoding the secreted heterologous polypeptide, and (ii) the mutant cell comprises a second nucleic acid which comprises a disruption or a deletion in a cyclohexadepsipeptide synthetase gene, wherein the mutant cell produces less cyclohexadepsipeptide than the parent Fusarium venenatum cell when cultured under the same conditions as a result of the disruption or the deletion in the cyclohexadepsipeptide synthetase gene, wherein the cyclohexadepsipeptide synthetase gene encodes a cyclohexadepsipeptide synthetase having an amino acid sequence which has at least 95% identity with SEQ ID NO: 2; or a cyclohexadepsipeptide synthetase which is encoded by a nucleic acid which hybridizes under at least high stringency conditions with (i) the nucleic acid of SEQ ID NO: 1, (ii) the cDNA of SEQ ID NO: 1, or (iii) a complete complementary strand of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 45° C. in 5×SSPE, 0.3% SDS, 2001 g/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 55° C.; and (b) isolating the secreted heterologous polypeptide from the cultivation medium.