Patent ID: 8802416

Claim:
A purified protease obtained from Bacillus subtilis isolate Rand, a representative sample of which has been deposited as CCTCC accession number M2013058, having the following properties: a) an apparent molecular weight of 28 kD determined by SDS-PAGE; b) a pH stability with buffers ranging from pH 5 to pH 11 at 25° C.; c) a working temperature in the range between 37° C. and 70° C. at pH 7.0; d) organic solvents stability of 25% (v/v) of organic solvents ranging from a log P of 0.8 to a log P of 8.8 for 30 min and at 37° C.; e) working inhibitors including ethylenediaminetetraacetic acid (EDTA), bestatin, pepstatin A, phenylmethanesulfonyl fluoride (PMSF), 1,4-Dithio- DL -threitol (DTT), and 2-Mercaptoethanol for 30 min at 37° C.; f) working metal ions such as K + , Na + , Mg 2+ , Ca 2+ , Mg 2+ , Fe 2+ , Co 2+ , Cu 2+ , Zn 2+ and Li 2+ and g) substrate specificity with natural substrate including casein, albumin, haemoglobin and azocasein, the stability of the purified protease is substantially 100%; with azocasein after 30 minutes incubation at 37° C.