Patent ID: 7959920

Claim:
A method of generating an antibody that specifically binds to a target parathyroid hormone (PTH) peptide, said method comprising: a) introducing a PTH peptide immunogen to a mammal in an amount sufficient to produce an antibody to said PTH peptide immunogen; wherein said PTH peptide immunogen has an amino acid sequence which starts at amino acid position 1 and ends at amino acid position 84 of a whole PTH; wherein the whole PTH has the amino acid sequence: (SEQ ID NO:1) Ser Val Ser Glu Ile Gln Leu Met His Asn Leu 1 5 10 Gly Lys His Leu Asn Ser Met Glu Arg Val Glu 15 20 Trp Leu Arg Lys Lys Leu Gln Asp Val His Asn 25 30 Phe Val Ala Leu Gly Ala Pro Leu Ala Pro Arg 35 40 Asp Ala Gly Ser Gln Arg Pro Arg Lys Lys Glu 45 50 55 Asp Asn Val Leu Val Glu Ser His Glu Lys Ser 60 65 Leu Gly Glu Ala Asn Lys Ala Asp Val Asn Val 70 75 Leu Thr Lys Ala Lys Ser Gln; 80 b) recovering said antibody from said mammal; c) purifying said antibody by a combination of a positive adsorption to a PTH peptide consisting of an amino acid sequence consisting of PTH 1-34 , and a negative adsorption to a PTH peptide having an amino acid sequence which starts at amino acid position 2 and ends at amino acid position 34 of said whole PTH.