Patent ID: 7026529

Claim:
A method of preparing transgenic shoots of a plant of the genus Taraxacum , the method comprising: (a) pre-incubating explants of said plant in a pre-incubation medium containing auxin, cytokinin, acetosyringone, glucose, sucrose, and betaine; (b) contacting and co-cultivating said plant explants with Agrobacterium cells comprising a DNA fragment of interest and an antibiotic selection marker operably linked to at least one T-DNA border in a pre-incubation medium, thereby producing transformed plant explants; and (c) culturing the transformed plant explants in a selection medium containing auxin, cytokinin, maltose, sucrose and antibiotic, thereby producing transformed plant shoots, wherein 0.05˜0.5 mM of acetosyringone and 1˜3% (w/v) of glucose are included in the pre-incubation media, and 0.5˜3% (w/v) of maltose is included in the selection medium.