Patent ID: 8721990

Claim:
An assay device for the determination of hCG in a liquid sample over an extended concentration rang, comprising a first assay and a second assay, wherein the first assay comprises a first flow-path having a sole first assay detection zone and a mobilisable, labeled, hCG-binding reagent provided upstream from the first assay detection zone; and the second assay comprises a second flow-path having a sole second assay detection zone, a mobilisable, labeled, hCG-binding reagent provided upstream from the second assay detection zone, and a mobilisable scavenger binding reagent for hCG provided upstream from the second assay detection zone; wherein the first and second assay detection zones each independently comprise an immobilised reagent that is capable of immobilising the respective labeled, hCG-binding reagent in the presence of hCG; wherein said first and second assays are provided on separate substrates, or on a common substrate but with the first and second flow-paths fluidically isolated from one another; wherein the immobilisation of the labeled, hCG-binding reagents at the detection zones provides an indication of hCG in said liquid sample; and wherein the first assay provides an indication of hCG in a first concentration range and the second assay provides an indication of hCG in a second, higher concentration range.