Patent ID: 8642283

Claim:
A method for measuring the ability of an agent to modulate the ability of isolated native Target of Rapamycin (TOR) protein to phosphorylate a TOR substrate, comprising determining the level of phosphorylation by said isolated native TOR of said TOR substrate under conditions in which said isolated native TOR and the TOR substrate interact: (a) in the absence of said agent; (b) in the presence of said agent; and comparing the level of phosphorylation of said TOR substrate from (a) with the level of phosphorylation from (b), wherein a difference in the level of phosphorylation of said TOR substrate from (a) and the level of phosphorylation from (b) indicates that the agent has the ability to modulate the ability of TOR protein to phosphorylate the TOR substrate, wherein said isolated native TOR causes the difference in the level of phosphorylation of said TOR substrate from (a) and the level of phosphorylation from (b).