Patent ID: 6962780

Claim:
A method for synthesis of nucleic acids which comprises: adding a sample comprising a cell, fungus, bacterium, or virus to an amplification reaction solution comprising a polyhydric alcohol and ammonium sulfate, wherein said cell, fungus, bacterium, or virus of said sample is intact and unlysed without extracting and/or purifying said nucleic acid from inside said cell, fungus, bacterium, or virus, when said sample is added to the amplification reaction solution, and directly amplifying said nucleic acid from said intact and unlysed cell, fungus, bacterium, or virus in said amplification reaction solution, said amplification being in a region rich in guanine (G) and cytosine (C) content, wherein said amplifying step consists of (i) preheating said amplification reaction solution, (ii) denaturing said nucleic acid, (iii) annealing a primer to said denatured nucleic acid, and (iv) polymerizing said primer, and wherein said steps(ii), (iii), and (iv) of said amplifiying step are repeated.