Patent ID: 8366935

Claim:
A method for the production of an extract of Phyllanthus comprising the steps of: (a) extracting Phyllanthus components with a solvent; (b) fractionating the extract obtained in the preceding step on the basis of hydrophobicity; (c) collecting and combining fractions that correspond in hydrophobicity to elute fractions resulting from 10-50% methanol elution steps, wherein said elute fractions are obtained by low pressure vacuum liquid chromatography (VLC) using a C18 reversed-phase lipophilic column and a water/methanol gradient system, wherein the gradient system is based on an initial elution step of loading said column with 100% water, on intermediate elution steps with gradually decreasing water content and on a terminal elution step loading said column with 100% methanol; and (d) optionally drying the extract obtained in step (c).