Patent ID: 7985553

Claim:
A method of cell type-specific isolation of actively translated mRNA, said method comprising (a) contacting a lysate or fraction of a heterogeneous population of cells with a reagent, said heterogeneous population of cells comprising a first subpopulation of cells comprising tagged ribosomes and a second subpopulation of cells not comprising said tagged ribosomes, wherein (i) said tagged ribosomes comprise a ribosomal protein fused to a peptide tag, said peptide tag binding specifically to said reagent, and said peptide tag not being a ribosomal protein; (ii) expression of said tagged ribosomal protein is under the control of a regulatory sequence which is endogenous to said first subpopulation of cells and which results in expression of said tagged ribosomal protein in said first subpopulation of cells but not in said second subpopulation of cells; and (iii) said tagged ribosomes are bound to said actively translated mRNA; and (b) isolating said actively translated mRNA from said lysate or fraction of the heterogeneous population of cells.