Patent ID: 8535900

Claim:
A method of preparing a lysophospholipid comprising treating a substrate comprising a phospholipid with at least one lipolytic enzyme to produce said lysophospholipid, wherein said lipolytic enzyme has phospholipase activity and wherein said lipolytic enzyme is obtainable from the genus Thermobifida , wherein phospholipase activity is determined by an assay comprising mixing the enzyme with a pH 7 buffered substrate solution containing phosphatidylcholine at 37 degrees C. for 10 minutes, then raising the temperature to 99 degrees C. to stop any reaction, and analyzing any resultant free fatty acid, whereby if there is resultant free fatty acid, enzymatic activity is calculated as micromole fatty acid produced per minute at pH 7 and the enzyme has phospholipase activity, wherein said lipolytic enzyme comprises at least one of a GDSx motif, or a GANDY block or a HPT block, and wherein said lipolytic enzyme comprises an amino acid sequence shown as SEQ ID NO: 5 or an amino acid sequence having at least 90% identity therewith.