Patent ID: 7695924

Claim:
A method of evaluating the state of a single cell comprising: permeabilizing said single cell; simultaneously measuring the activation state in said cell of a first protein within the Mitogen-activated protein kinase (MAPK), protein kinase B (AKT), Signal Transducers and Activators of Transcription (STAT), nuclear factor kB (NFkB), protein kinase C (PKC) or wingless-type MMTV integration site family (WNT) signaling pathways and a second protein within the MAPK, AKT, STAT, NFkB, PKC or WNT signaling pathways by contacting said single cell with at least two distinguishably labeled activation state-specific antibodies, wherein a first of said at least two distinguishably labeled activation state-specific antibodies is specific for said first protein and a second of said at least two distinguishably labeled activation state-specific antibodies is specific for said second protein, and detecting the presence or absence of binding of said at least two distinguishably labeled activation state-specific antibodies to said first protein and said second protein, wherein said first protein and said second protein are different proteins; and evaluating the state of said single cell based on said measuring of said activation state of said first protein and said second protein.