Patent ID: 7812149

Claim:
A composition comprising first and second chemically synthesized oligomeric compounds, wherein: the first and second chemically synthesized oligomeric compounds are not covalently linked to each other; each of the first and second oligomeric compounds consists of 20 to 24 linked nucleosides; the first oligomeric compound is 100% complementary to the second oligomeric compound and to a selected target messenger RNA; the first oligomeric compound has the formula: (Z 1 ) n —X 1 —Y 1 —X 2 —Y 2 —X 3 —Y 3 —X 4 —Y 4 —(Z 2 ) m wherein: each Z 1 is, independently, a natural or modified nucleoside; X 1 is a region of two 2′-F modified nucleosides; Y 1 is a region of one 2′-O-methyl modified nucleoside; X 2 is a region of two 2′-F modified nucleosides; Y 2 is a region of two 2′-O-methyl modified nucleosides; X 3 is a region of five 2′-F modified nucleosides; Y 3 is a region of three 2′-O-methyl modified nucleosides; X 4 is a region of three 2′-F modified nucleosides; Y 4 is a region of two 2′-O-methyl modified nucleosides; each Z 2 is, independently, a natural or modified nucleoside; and n and m are each, independently, 0, 1, or 2; and the second oligomeric comprises a plurality of ribose nucleoside subunits.