Patent ID: 8110359

Claim:
A method of identifying a serotype of Klebsiella pneumoniae , the method comprising: (1) extracting DNA from a sample of Klebsiella pneumoniae for use as a DNA template; (2) performing polymerase chain reaction (PCR) using the DNA template obtained from Step (1), and pair sets of PCR primers comprising a primer set selected from the group consisting of a first primer set, a second primer set, a third primer set, a fourth primer set, a fifth primer set, a sixth primer set, a seventh primer set and a eighth primer set; and (3) identifying a K57 serotype of Klebsiella pneumoniae if PCR products are found from the first or the second primer sets, or identifying a NTUH-N1 serotype of Klebsiella pneumoniae if PCR products are found from the third, the fourth, the fifth, the sixth, the seventh or the eighth primer set; wherein the first primer set is consisting of SEQ ID NO:21 and SEQ ID NO:22, the second primer set is consisting of SEQ ID NO:23 and SEQ ID NO:24, the third primer set is consisting of SEQ ID NO:25 and SEQ ID NO:26, the fourth primer set is consisting of SEQ ID NO:27 and SEQ ID NO:28, the fifth primer set is consisting of SEQ ID NO:29 and SEQ ID NO:30, the sixth primer set is consisting of SEQ ID NO:31 and SEQ ID NO:27, the seventh primer set is consisting of SEQ ID NO:32 and SEQ ID NO:33, and the eighth primer set is consisting of SEQ ID NO:34 and SEQ ID NO:35.