Patent ID: 8828660

Claim:
A method of detecting multiple types of human papillomavirus (HPV) nucleic acid present in a biological sample, comprising the steps of: (a) contacting nucleic acid in a biological sample with a mixture of amplification oligomers, each of which amplifies a HPV sequence in an E6/E7 target region sequence, in which the mixture comprises: (i) first amplification oligomers comprising nucleic acid sequences selected from the group consisting of SEQ ID Nos. 18, 20, 22, 24, 28, 30, 32, 34, 36, RNA equivalents thereof, complements thereof, and combinations thereof; and (ii) second amplification oligomers comprising nucleic acid sequences selected from the group consisting of SEQ ID Nos. 38, 39, 40, 41, RNA equivalents thereof, complements thereof, and combinations thereof; (b) amplifying a HPV sequence from the target region sequence in at least one HPV type by using the amplification oligomers and a nucleic acid polymerase in vitro to produce an HPV amplified product; and (c) detecting the amplified product by using a detection probe oligomer that is sufficiently complementary to hybridize specifically with the HPV amplified product to indicate the presence in the sample of at least one type of HPV nucleic acid.