Patent ID: 8597908

Claim:
An isolated DNA sequence comprising: (a) a DNA sequence encoding a Staphylococcus aureus protein A or a variant protein thereof having at least 90% amino acid identity to SEQ ID NO: 2, wherein said variant protein has immunoglobulin-binding activity; and (b) a promoter which is operatively linked to said DNA sequence encoding a Staphylococcus aureus protein A or a variant protein thereof, wherein said promoter has a promoter activity when said isolated DNA sequence is transformed into a Brevibacillus genus bacterium wherein the promoter is a promoter of a cell wall protein of a Brevibacillus genus bacterium, and wherein the isolated DNA sequence further comprises downstream of the promoter, a Shine-Dalgarno sequence which is capable of functioning in a Brevibacillus genus bacterium and a secretion signal peptide-encoding DNA sequence which is capable of functioning in a Brevibacillus genus bacterium.