Patent ID: 7504250

Claim:
A recombinant microorganism, useful for the production of 1,3-propanediol, comprising: (a) at least one gene encoding a glycerol or diol dehydratase α subunit selected from the group consisting of SEQ ID NO:69, SEQ ID NO:74, SEQ ID NO:79, SEQ ID NO:82, SEQ ID NO:87, SEQ ID NO:91, and SEQ ID NO:96; at least one gene encoding a glycerol or diol dehydratase β subunit selected from the group consisting of SEQ ID NO:70, SEQ ID NO:75, SEQ ID NO:80, SEQ ID NO:83, SEQ ID NO:88, SEQ ID NO:92, and SEQ ID NO:97; and at least one gene encoding a glycerol or diol dehydratase γ subunit selected from the group consisting of SEQ ID NO:71, SEQ ID NO:76, SEQ ID NO:81, SEQ ID NO:84, SEQ ID NO:89, SEQ ID NO:93, and SEQ ID NO:98; (b) at least one gene encoding the OrfX polypeptide sequence selected from the group consisting of SEQ ID NO:72, SEQ ID NO:77, SEQ ID NO:85, SEQ ID NO:94, and SEQ ID NO:99; and at least one gene encoding the OrfZ polypeptide sequence selected from the group consisting of SEQ ID NO:73, SEQ ID NO:78, SEQ ID NO:86, SEQ ID NO:90, SEQ ID NO:95, and SEQ ID NO:100; and (c) at least one gene encoding a polypeptide having non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol, said polypeptide having the amino acid sequence of SEQ ID NO:57; wherein, if present, the dhaT gene encoding a 1,3-propanediol oxidoreductase activity has a mutation inactivating the gene and the microorganism is selected from the group consisting of Citrobacter freundii, Clostridium pasteurianum, Klebsiella pneumoniae, Klebsiella oxytoca , and Escherichia coli , provided that, when the microorganism is Citrobacter freundii, Clostridium pasteurianum, Klebsiella pneumoniae , or Klebsiella oxytoca the at least one gene of (c) is an exogenous gene.