Patent ID: 8450056

Claim:
An arrayed imaging reflectometry (AIR) sensor chip suitable for the detection of antiviral immune responses comprising: a multilayer substrate comprising a surface and virus-like particles (VLPs) or viral capsid (CA) fragments from different viral strains or isolates, wherein said VLPs or CA fragments from each individual isolate or strain are covalently attached to the chip surface at different locations; wherein said VLPs or CA fragments retain conformational epitopes that are capable of binding to antiviral antibodies; wherein said surface with VLPs or CA fragments covalently attached at the different locations forms a coating on the chip surface that results in destructive interference of polarized light illuminating said surface at an appropriate incident angle and wavelength in the absence of antibody binding; and wherein exposure of the surface to a sample comprising antiviral antibodies produces a detectable change in reflectance upon antigen-antibody binding.