Patent ID: 8101354

Claim:
A method for screening for a genotype for a Tobiano coat color pattern, comprising: obtaining a biological sample containing a nucleic acid sample from an equine animal, said nucleic acid sample comprising equine chromosome ECA3q; and identifying a chromosomal inversion in a chromosome ECA3q, said inversion having a telomeric breakpoint located between a KIT gene and a KDR gene in a band ECA3q21 of said chromosome ECA3q and a centromeric breakpoint located between a ADH1D gene and a UNC5C gene in a band ECA3q13 of said chromosome ECA3q; said step of identifying a chromosomal inversion comprising detecting at least one of a nucleic acid sequence surrounding the telomeric breakpoint of the inverted ECA3q chromosome and a nucleic acid sequence surrounding the centromeric breakpoint of the inverted ECA3q chromosome, or sequences complementary thereto; wherein the presence of the chromosomal inversion as indicated by detection of at least one of said telomeric breakpoint or said centromeric breakpoint indicates that the equine animal possesses a genotype for a Tobiano coat color pattern.