Patent ID: 7521192

Claim:
A method for identifying a compound that modulates EDG-modulated lymphocyte activation, the method comprising the steps of: (i) contacting a lymphocyte comprising a recombinant EDG polypeptide with the compound, the EDG polypeptide having at least 95% identity to an amino acid sequence of SEQ ID NO:5 and further wherein the EDG polypeptide inhibits lymphocyte activation-induced upregulation of CD69 expression; (ii) stimulating a T cell receptor or a B cell receptor on said lymphocyte, thereby activating the lymphocyte; and (iii) determining the effect of the compound upon EDG-modulated activation of the activated lymphocyte comprising the EDG polypeptide in comparison to an activated lymphocyte comprising a recombinant EDG polypeptide not contacted by the compound, thereby identifying a compound that modulates EDG-modulated lymphocyte activation.