Patent ID: 8211691

Claim:
A methylotrophic yeast host cell that does not display an alpha-1,6 mannosyltransferase activity with respect to an N-glycan on a glycoprotein comprising a nucleic acid encoding an α-1,2-mannosidase catalytic domain fused to a cellular targeting signal peptide not normally associated with the catalytic domain, wherein said signal peptide is selected to target the α-1,2-mannosidase catalytic domain to the ER or Golgi apparatus of the host cell, and wherein the host cell produces glycoproteins comprising N-glycans, wherein 50-100 mole % of the N-glycans attached to the glycoproteins have a Man5GlcNAc2 oligosaccharide structure that can serve as a substrate for N-acetylglucosamine (GlcNAc) transferase (GnT I) in vivo.