Patent ID: 6951931

Claim:
A pair of primers for amplification or detection of a nucleic acid, at least one of said primers comprising a compound of the formula B x —R 1 , or a tautomer, solvate or salt thereof, wherein R 1 is a binding partner and B x is of the subformula: wherein R 2 is A(Z) X1 , wherein A is a spacer and Z independently is a label bonding group optionally bonded to a detectable label, but R 2 is not an amine, protected amine, nitro or cyano; each R 27 is independently —CH═, —N═, —C(C 1-8 alkyl)= or —C(halogen)=, but no adjacent R 27 are both —N═, or two adjacent R 27 are taken together to form a ring having the structure: where R a is independently —CH, —N═, —C(C 1-8 alkyl)= or —C(halogen)=, but no adjacent R a are both —N═; and R 34 is —O—, —S—, or —N(CH 3 )—; and X1 is 1, 2 or 3.