Patent ID: 8026051

Claim:
A method comprising: a) contacting a sample with a combination of PNA probes, wherein the combination of PNA probes comprises the probing nucleobase sequences consisting of SEQ ID NO: 1-23: AGA-GAG-CAG-CAT-GCA, (Seq. Id. No. 1) AGA-GAG-CAA-CAT-GCA, (Seq. Id. No. 2) ACA-GCA-GAA-GCC-GTG, (Seq. Id. No. 3) CAT-AAA-TGG-CTA-CCA-GA, (Seq. Id. No. 4) CAT-AAA-TGG-CTA-CCC-AG, (Seq. Id. No. 5) ACT-TGG-AGT-CGA-TAG, (Seq. Id. No. 6) CCA-AGG-CTT-ATA-CTC-GC, (Seq. Id. No. 7) CCC-CTG-AAT-CGG-GAT, (Seq. Id. No. 8) GAC-GCC-AAA-GAC-GCC, (Seq. Id. No. 9) ATC-GTC-AGA-GGC-TAT-AA, (Seq. Id. No. 10) TAG-CCA-GAA-GAA-AGG, (Seq. Id. No. 11) CAT-AAA-TGG-CTA-GCC-AG, (Seq. Id. No. 12) CTC-CGA-TGT-GAC-TGC-G, (Seq. Id. No. 13) TCC-CAG-ACT-GCT-CGG, (Seq. Id. No. 14) TCC-AAG-AGG-TCG-AGA, (Seq. Id. No. 15) GCC-AAG-CCA-CAA-GGA, (Seq. Id. No. 16) GCC-GCC-AAG-CCA-CA, (Seq. Id. No. 17) GGA-CTT-GGG-GTT-AG, (Seq. Id. No. 18) CCG-GGT-GCA-TTC-CA, (Seq. Id. No. 19) ATG-TAG-AAC-GGA-ACT-A, (Seq. Id. No. 20) GAT-TCT-CGG-CCC-CAT-G, (Seq. Id. No. 21) CTG-GTT-CGC-CAA-AAA-G, (Seq. Id. No. 22) and AGT-ACG-CAT-CAG-AAA, (Seq. Id. No. 23) or the complements thereof; and b) detecting, identifying and/or quantifying hybridization of one or more of the PNA probing nucleobase sequences to the target sequence of one or more species of Candida yeast in the sample, under suitable hybridization conditions or suitable in-situ hybridization conditions, and correlating the result with the presence, absence and/or quantity of Candida yeast in the sample.