Patent ID: 8685899

Claim:
A multi-labeled polymeric scaffold to which a plurality of label molecules capable of emitting or producing a detectable signal is attached, wherein the multi-labeled polymeric scaffold comprises an oligonucleotide extension sequence comprising a 5â€² phosphate group which is capable of hybridization bonding to a nucleic acid sequence, wherein the multi-labeled polymeric scaffold is a linear dendritic polynucleotide composition having a plurality of single stranded regions to which one or more labeled oligonucleotides are hybridized, said linear dendritic polynucleotide composition comprising first, second and third polynucleotide monomers bonded together by hybridization in a 5â€²-3â€² orientation, each polynucleotide monomer, prior to being hybridization bonded to one another, having first, second and third single stranded hybridization regions; and in said linear dendritic polynucleotide composition the third single stranded hybridization region of the first polynucleotide monomer being hybridization bonded to the first single stranded hybridization region of the second polynucleotide monomer, and the third single stranded hybridization region of the second polynucleotide monomer being hybridization bonded to the first single stranded hybridization region of the third polynucleotide monomer, and the first single strand region of the first polynucleotide monomer is capable of hybridization bonding to the nucleic acid sequence, wherein the second single stranded hybridization regions within said linear dendritic polynucleotide composition are hybridization bonded to one or more labeled oligonucleotides comprising one or more label molecules and wherein the multi-labeled polymeric scaffold has a total molecular weight of about 50 to about 350 kDa.