Patent ID: 6846624

Claim:
A process for isolating a vector that contains a gene encoding a protein having a zinc finger structure or which binds zinc, comprising culturing a host cell on a culture plate, wherein the host cell contains an expression vector which contains an uncharacterized DNA, expressing the vector to produce a protein, transferring the protein in the host cell to a film by contacting the host cell on the culture plate with the film, peeling the film from the culture plate, treating the protein on the film with a protein denaturant, washing the film, regenerating the protein on the film with a regenerating solution, adding a labeled zinc ion to the film which can bind to the protein, detecting the labeled zinc ion bound to the protein, and isolating the vector containing the uncharacterized DNA from host cells at a location on the culture plate which corresponds to the location of the protein-bound labeled zinc ion on the film.