Patent ID: 8691210

Claim:
A method for activating an antigen-presenting cell, which comprises: a. transducing an antigen-presenting cell with a nucleic acid comprising a promoter operably linked to a nucleotide sequence that encodes a chimeric protein, wherein the chimeric protein comprises (i) a membrane targeting region, (ii) a multimeric FKBP ligand-binding region, and (iiia) a cytoplasmic region of a pattern recognition receptor (PRR) selected from the group consisting of NOD1 PRR, NOD2 PRR, and RIG-1 PRR or (iiib) MyD88, wherein the chimeric protein is expressed in the antigen-presenting cell; and b. contacting the antigen-presenting cell with a non-peptidic multimeric ligand that binds to the multimeric FKBP ligand-binding region; whereby the NF-κB pathway is activated and the antigen-presenting cell is activated.