Patent ID: 8067673

Claim:
A method for transforming an okra plant, okra plant cells or okra plant tissues, said method comprising: (a) surface sterilizing seeds of an okra plant, and optionally imbibing the seeds in water; (b) isolating immature or mature embryos from the seeds; (c) wounding the embryos at the plumule end; (d) co-cultivating the wounded embryos of step (c) in a medium containing a recombinant Agrobacterium strain which comprises a selectable marker gene, said Agrobacterium strain optionally further comprising DNA or RNA sequences of interest; (e) culturing the embryos of step (d) on a tissue culture medium comprising zeatin and a selection agent to select transformed okra plant cells or tissues; (f) culturing the transformed okra plant cells or tissues of step (e) on a suitable tissue culture medium comprising zeatin to obtain shoot buds; and (g) culturing the shoot buds of step (f) in a hormone-free rooting medium to obtain rooted transformed okra plants.