Patent ID: 6913884

Claim:
A method comprising: a) providing a composition camprising first primers and at least 10 different target nucleic acids, wherein either said first primers or said at least 10 different target nucleic acids are immobilized to at least one solid support; b) performing a first analysis of said at least 10 different target nucleic acids, said first analysis comprising: i) contacting said first primers with said at least 10 different target nucleic acids whereby at least one of said first primers hybridizes with said at least 10 different target nucleic acids; ii) removing unhybridized first primers; and iii) contacting said hybridized first primers with an enzyme such that said hybridized first primers are modified forming first modified primers, whereby a sinnal is obtained from said first analysis and whereby said at least 10 different target nucleic acids is not consumed, and c) performing a second analysis of said at least 10 different target nucleic acids, comprising: i) contacting second primers with said at least 10 different target nucleic acids whereby at least one of said second primers hybridizes with said at least 10 different target nucleic acids; ii) removing unhybridized second primers; and iii) contacting said hybridized second primers with an enzyme such that said hybridized second primers are modified forming second modified primers, whereby a signal is obtained from said second analysis and wherein a signal obtained from said second analysis is not diminished more than 40% compared to a signal obtained from said first analysis.