Patent ID: 7270815

Claim:
An isolated 2-O sulfatase produced by expressing an isolated nucleic acid molecule selected from the group consisting of: (a) nucleic acid molecules which hybridize to a nucleic acid molecule having a nucleotide sequence selected from the group consisting of nucleotide sequences set forth as SEQ ID NOs: 1 and 3, wherein the hybridization conditions are 1) hybridization at 65° C. in hybridization buffer that consists of 3.5×SSC, 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% Bovine Serum Albumin, 2.5 mM NaH 2 PO 4 , pH7, 0.5% SDS, 2 mM EDTA, —wherein SSC is 0.15M sodium chloride/0.015M sodium citrate, pH7; SDS is sodium dodecyl sulphate; and EDTA is ethylenediaminetetracetic acid and 2) washing in 2×SSC at room temperature and then in 0.1–0.5×SSC/0.1×SDS at 68° C., (b) nucleic acid molecules that differ from the nucleic acid molecules of (a) in codon sequence due to degeneracy of the genetic code, and (c) full complements of (a) or (b).