Patent ID: 8211688

Claim:
A process for producing a substance, which comprises the steps of: selecting an isolated microorganism in which the activities of glutamine-synthetase adenylyltransferase and PII regulatory protein for glutamine synthetase are lost, and which has the ability to form and accumulate the substance, and wherein the microorganism is an Escherichia coli in which at least one nucleotide is deleted, substituted or added in the nucleotide sequence of the gene encoding wild-type glutamine synthetase adenylyltransferase thereby losing such activity, and in which at least one nucleotide is deleted, substituted or added in the nucleotide sequence of the gene encoding wild-type PII regulatory protein for glutamine synthetase thereby losing such activity; culturing the Escherichia coli in a medium; allowing the substance to form and accumulate in the medium; and recovering the substance from the medium, wherein said substance is L-glutamine, an amino acid, or a nucleotide biosynthesized using nitrogen supplied from said L-glutamine.