Patent ID: 6916663

Claim:
A method for detecting proteins on polyacrylamide gels, comprising the steps of: (a) sensitizing the proteins with a dye composition to form a protein dye complex, containing one or more acidic organic dyes selected from the group consisting of calcon dye derivatives, alizarin yellow GG, alizarin yellow R, zincon, Bordeaux R, tryphan blue, and acid blue 92 as a silver ion sensitizing agent, and a basic organic dye selected from the group consisting of brilliant cresyl blue ALD, direct blue 71, crystal violet, methyl violet B base, methyl violet 10B, methyl violet B, ethyl green, rhodamine B, ethyl violet, phenosafranine and methylene blue as an auxiliary agent; and then, washing them; (b) impregnating silver ions to complexes of the protein-dye, and then, reducing them to metallic silver; and (c) observing the protein-dye-metallic silver complexes.