Patent ID: 8603745

Claim:
A method of generating a positive control sample for detecting cystic fibrosis, the method comprising: providing a composition comprising a synthetic target sequence comprising at least one CFTR mutation and a synthetic target control sequence that includes a region of a CFTR gene which does not include the at least one CFTR mutation, wherein the at least one CFTR mutation comprises a ΔF508, ΔI507, 621+1G>T, G85E, 1078delT, R553X, G542X, R117H, R334W, 3849+10kb C>T, R1162X, G551D, 1717−1G>A, R347P, 2789+5G>A, 2184delA, W1282X, A455E, 711+1G>T, 3659delC, 3120+1G>A, N1303K, R560T, 1898+1G>A, or I148T CFTR mutation; and carrier DNA, wherein the carrier DNA is at a concentration of at least 50 μg/20 μl in the composition, thereby generating a positive control sample for detecting cystic fibrosis.