Patent ID: 7536045

Claim:
A method of analysis of an array image including one or more luminous spots on a background, comprising: operating an array localization system implementing morphological filtering by: determining a shape and location of each spot on the array image; generating a binary map of pixels defining a boundary of each spot on the background; isolating each spot from the background by an extraction operation using said binary map; examining each spot by a segmentation operation to identify pixels belonging to a same cluster according to a preestablished criterion; and for each spot, defining relative characteristic parameters and quality indexes determined in function of gray levels of pixels of the spot; wherein generating said binary map comprises: filtering the array image with at least a morphological filter generating only a single corresponding marker image of the background; determining a background level by carrying out a reconstruction operation on said single corresponding marker image to generate a corresponding reconstructed image of the background; generating a filtered image from which the luminosity of the background is removed by performing a top-hat operation on said reconstructed background image and the array image; and performing a thresholding operation on said filtered image of the background luminosity.