Patent ID: 7148048

Claim:
A process for purification of recombinant human porphobilinogen deaminase (rhPBGD) on an industrial scale from a rhPBGD containing extract obtained from a fermentation of a recombinant cell capable of expressing rhPBGD, characterized by following steps: (i): prior to any other chromatography column purification step, loading the rhPBGD containing extract on an equilibrated Cibacron Blue 3G sepharose affinity chromatography column having a column volume of at least 5 L and, after adequate washing step(s), eluting a sample comprising rhPBGD; (ii) : loading the eluent of step (i) on an equilibrated chromatography column having a column volume of at least 5 L and, after adequate washing step(s), eluting a sample comprising rhPBGD; (iii) : performing one or more further chromatography column step(s) wherein the chromatography column is a column relying on a different principle than an affinity chromatography column and also relying on a different principle than the column used in step (ii), eluting a fraction comprising rhPBGD, and collecting said fraction, where said rhPBGD has porphobilinogen deaminase activity and is bound by Cibacron Blue 3G.