Patent ID: 8288322

Claim:
A library comprising a collection of members of a family, the family comprising a diversity of antibodies or functional fragments thereof, wherein the antibodies or functional fragments thereof each comprise a VH CDR1, a VH CDR2, a VH CDR3 and framework regions and the antibodies or functional fragments are encoded by DNA sequences comprising sequences encoding (a) the VH CDR1, wherein the VH CDR1 comprises the amino acid sequence according to the formula -X 1 -Y-X 2 -M-X 3 - (SEQ ID NO:636), wherein X 1 , X 2 , and X 3 are independently selected from the group consisting of A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, and Y, (b) the VH CDR2, wherein the VH CDR2 comprises the amino acid sequence according to the formula X 4 -I-X 5 -X 6 -S-G-G-X 7 -T-X 8 -Y-A-D-S-V-K-G- (SEQ ID NO:637), wherein X 4 and X 5 are independently selected from the group consisting of Y, R, W, V, G, and S, X 6 is selected from the group consisting of P and S, and X 7 and X 8 are independently selected from the group consisting of A, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, and Y, (c) the VH CDR3, wherein the sequence encoding the VH CDR3 is from the CDR3 region of an immunoglobulin gene of a B cell, and (d) the framework regions, wherein the framework regions comprises VH 3-23 framework regions or VH3-23 related germline framework regions.