Patent ID: 7387884

Claim:
A method for preparing a yeast-derived extract solution for cell-free protein synthesis, said method comprising: freezing yeast cells to obtain frozen yeast cells; rupturing said frozen yeast cells to obtain ruptured frozen yeast cells; extracting said ruptured frozen yeast cells with a buffered solution for extraction containing a protease inhibitor and diothiothreitol to obtain a liquid containing a yeast-derived extract; removing residue of the yeast cells and intracellular components having a molecular weight of not more than 5,000 from said liquid containing the yeast extract to obtain an extract solution containing yeast-derived extract; and concentrating said extract solution containing the yeast-derived extract to obtain the yeast extract solution for cell-free protein synthesis, wherein said concentrated yeast extract solution for cell-free protein synthesis has an absorbance at 280 nm of 35-100.