Patent ID: 6846630

Claim:
A method for detecting diagnostically a nucleic acid encoding FLT3 kinase and having a tandem duplication mutation present in a nucleotide sequence of a juxtamembrane domain, comprising: step (a) preparing a human nucleic acid sample; step (b): subjecting the nucleic acid sample obtained in step (a) to a gene amplification reaction, wherein a nucleic acid fragment comprising exon 11 or exons 11 to 12 of the FLT3 gene and having a tandem duplication mutation in the juxtamembrane domain is amplified; and step (c): detecting the presence of the tandem duplication mutation in the nucleic acid fragment of said step (b), wherein the presence of a tandem duplication mutation is indicative of a disease selected from the group consisting of leukemia or Myelodysplastic syndrome, wherein said disease is associated with tandem duplication mutation in a nucleotide sequence of the juxtamembrane domain.