Patent ID: 8324367

Claim:
A deoxyribonucleic acid (DNA) sequence comprising: (1) a first sequence, said first sequence having a 5′ end and a 3′ end; and (2) a second sequence, said second sequence having a 5′ end and a 3′ end; and (3) a first pair of flanking sequences, wherein a first member of the first pair is located upstream of the 5′ end of the first sequence and a second member of the first pair is located downstream of the 3′ end of the second sequence; and (4) a second pair of flanking sequences, wherein a first member of the second pair is located downstream of the 3′ end of a first member of the first pair and upstream of the 5′ end of the first sequence; and a second member of the second pair is located downstream of the 3′ end of the first sequence and upstream of the 5′ end of the second sequence; wherein: one of the first sequence or the second sequence encodes a siRNA or shRNA capable of reducing mutated huntingtin and the other of the first sequence or the second sequence encodes a brain-derived neurotrophic factor (BDNF), the first sequence is excisable from said deoxyribonucleic acid sequence upon exposure of said deoxyribonucleic acid sequence to a second sequence modifier that recognizes the second pair of flanking sequences, and either: a) the second sequence is excisable from said deoxyribonucleic acid sequence upon exposure of said deoxyribonucleic acid sequence to a first sequence modifier that recognizes the first pair of flanking sequences, or b) both the first and second sequences are excisable together from the said deoxyribonucleic acid sequence upon exposure of said deoxyribonucleic acid sequence to the first sequence modifier; and wherein the first member of the first pair of flanking sequences is located upstream of the 5′ end of the first member of the second pair.