Patent ID: 6994954

Claim:
A method of procuring, preserving, transplanting and/or bloodless surgery involving an organ or tissue, comprising the steps of: (a) providing a base electrolyte solution, comprising: Na + ; K + ; Ca ++ ; Mg ++ ; Cl − ; H 2 PO 4 − ; HCO 3 − ; HEPES; Lactobionate; Sucrose; Mannitol; Glucose; Gluconate; Dextran 40; Adenosine; and optionally Glutathione; (b) mixing a first portion of the base solution with a first additive to produce a first mixed solution; (c) contacting the first mixed solution with an organ or tissue during at least one stage of procurement, preservation, transplantation, or bloodless surgery of that organ or tissue; (d) mixing a second portion of the base solution with a second, different additive to produce a second, different mixed solution; and (e) contacting the second mixed solution with an organ or tissue during at least one stage of procurement, preservation, transplantation, or bloodless surgery of that organ or tissue.