Patent ID: 8101169

Claim:
A method of delivering a nucleic acid to an ocular region of a subject, comprising: providing a nucleic acid and target cells or target tissue, the target cells being an autologous or allogeneic cell line or being isolated from an autologous or allogeneic individual and the target tissue being from an autologous or allogeneic individual; applying a voltage of about 100 V to about 10 kV for a duration of about 20 μsec to about 100 msec to an electrode to generate both an electric field and a mechanical stress wave, the electrode positioned to provide both the electric field and the mechanical stress wave to the target cells or target tissue sufficient to transfect the nucleic acid into the cells or the tissue; and transplanting the transfected cells or the transfected tissue into an ocular region of the subject, wherein the nucleic acid is expressed in the ocular region.