Patent ID: 7846896

Claim:
A composition comprising conjugates consisting of a N-terminal 447 amino acids of the Hepatocyte Growth Factor (HGF/SF)α-chain (NK4) molecule and a single polyethylene glycol group having a molecular weight of from about 30 to about 40 kDa, wherein said polyethylene glycol group has the formula and said —CO group randomly forms an amide bond with one of the amino groups of the N-terminal fragment of said NK4 molecule, wherein y is from 1 to 10; n and p together are from about 450 to about 950; and R is (C 1 -C 6 )alkyl, wherein said monoPEGylated conjugates comprise at least 92% of the total of pegylated NK4 molecules and unpegylated NK4 molecules in the composition, and further comprising 1 to 30 mg/ml monoPEGylated NK4, 150 mM NaCl, 10 mM sodium phosphate and a pH of 7.2.