Patent ID: 8753873

Claim:
A multiplexed microarray comprising: a substrate having a plurality of microarrays formed on an upper surface of the substrate; a chamber layer attached to the upper surface of the substrate, the chamber layer having a plurality of apertures formed therethrough, the plurality of apertures and the upper surface of the substrate cooperating to form a plurality of wells, each of the wells having a corresponding microarray of the plurality of microarrays located therein; a first channel layer disposed on the chamber layer, the first channel layer having a plurality of first fluid channels, each of the first fluid channels being superimposed over a corresponding aperture of the plurality of apertures of the chamber layer; and a port layer coupled to an upper surface of the first channel layer, the port layer having a plurality of fill ports formed therethrough, each of the fill ports being superimposed over a corresponding first fluid channel of the plurality of first fluid channels of the first channel layer such that each of the fill ports is in fluid communication with a corresponding well of the plurality of wells of the chamber layer through the corresponding first fluid channel of the plurality of first fluid channels of the first channel layer, wherein each fluid channel of the plurality of first fluid channels comprises an elongated slit and an opening fluidically coupled to a lateral end of the elongated slit, the opening having a width greater than the width of the elongated slit, and wherein each of the fill ports of the port layer is superimposed over an opening of the corresponding first fluid channel.