Patent ID: 8435503

Claim:
A process to improve viability, growth, and differentiation of corneal epithelial cells, the process comprising: applying a trophic composition to an external surface of a cornea of an eye of a human or an animal, wherein the trophic composition has a pH between 7.3 and 7.5 and an osmolarity between 300 and 350 mOsm and the trophic composition comprises the following components: Component Concentration (mg/L) Water q.s. Sodium chloride 6800 Glutamine 1754.4 Sodium bicarbonate 1160 Glucose 1080 Arginine HCl 421.4 Sodium acetate 300 Disodium phosphate 284 Leucine 131.2 Serine 126.1 Mg chloride 120.0 K chloride 112 Valine 70.3 Sodium pyruvate 55 Lysine HCl 54 Histidine HCl 50 Cysteine HCl 42 Adenine 24 Threonine 24 Ca chloride 20.05 Inositol 18 Glutamic acid 14.8 Asparagine 14.2 Methionine 13.5 Tyrosine 11.7 Phenylalanine 10.0 Tryptophan 9.3 Alanine 9.2 Glycine 7.6 Isoleucine 6.0 Aspartic acid 4.0 Sodium sulfate 3.4 Ferrous sulfate 0.003 Folic acid 0.8 Thymidine 0.73 Cyanocobalamin 0.41 Calcium pantothenate 0.3 Thiamine HCl 0.3 Thioctic acid 0.3 Zinc sulfate 0.144 Sodium silicate 0.142 Pyrodixine HCl 0.06 Niacinamide 0.04 Riboflavin 0.3 Biotin 0.02 Copper sulfate 0.003 Ammonium molybdate 0.00120 Ammonium vanadate 0.003 Mn chloride 0.00002 Sodium hyaluronate 70 Polyhexanide or 0.1 polyhexamethylene biguanide n-acetylcysteine 500 Hydroxyproline or proline 35.