Patent ID: 8404930

Claim:
A method for increasing transformation frequency in a monocot plant cell comprising: (a) introducing a nucleic acid into a monocot plant cell of an explant of an immature embryo by Agrobacterium -infection to produce an infected plant cell, wherein the plant cell is from a plant selected from the group consisting of maize, sorghum, barley, oats, rye, wheat, rice and triticale; (b) co-cultivating the infected plant cell for about 1 to 7 days on a co-cultivation medium comprising an auxin and about 0.1 μM copper; (c) culturing the infected cell for about 1 to 7 days on a resting medium to produce transformed tissue expressing the nucleic acid, wherein the resting medium comprises an auxin, a cytokinin, and about 1 μM to about 50 μM of copper, wherein transformation frequency is increased by at least 20% compared to transformation frequency of a control transformed cell that is not co-cultivated on the co-cultivation medium of (b) and not cultured on the resting medium of (c); and (d) regenerating the transformed tissue on a regeneration medium to produce a transformed monocot plant.