Patent ID: 8158349

Claim:
A method for purifying or isolating nucleic acids from a sample comprising: a) providing a device comprising: a first hollow body with an inlet opening for receiving a sample and an outlet opening, a second hollow body with an inlet opening and an outlet opening, wherein the inlet opening of the second hollow body is functionally connected to the outlet opening of the first hollow body, the first and the second hollow body can be detached from one another, a nucleic acid-binding material is located inside the second hollow body and above the outlet opening of the second hollow body, the second hollow body has a smaller volume compared to the first hollow body and the first hollow body has a volume of more than 5 ml, a first receiving vessel for receiving the second hollow body while the inlet opening of the second hollow body is functionally connected to the outlet opening of the first hollow body, wherein the first receiving vessel comprises means for applying an underpressure to the outlet opening of the second hollow body, b) transferring the sample into the device through the inlet opening in the first hollow body, c) passing the sample from the second hollow body through the nucleic acid-binding material into the first receiving vessel such that the nucleic acids bind to the nucleic acid-binding material, d) optionally washing the nucleic acids bound to the nucleic acid-binding material after step c), e) detaching the second hollow body from the first hollow body, f) optionally washing the nucleic acids bound to the nucleic acid-binding material after step e), and g) eluting the nucleic acids bound to the nucleic acid-binding material whereby the nucleic acids are collected in a second receiving vessel.