Patent ID: 7893316

Claim:
A method for reconstituting a non-human mammalian embryo in vitro, wherein said method consists of: (i) treating a non-human mammal diploid nucleus of a somatic donor cell, said treatment consisting of: a) controlled proteolysis of nuclear non-histone proteins and surrounding cytoskeleton proteins of the nucleus, wherein the controlled proteolysis must not cause lysis of the nucleus; and b) induction of an isomorphic swelling of the nucleus after controlled proteolysis of step a), by treatment consisting of treating with a polyanion; and (ii) transferring said treated nucleus into the cytoplasm of a recipient oocyte during metaphase II or interphase, wherein the recipient oocyte is enucleated thereby forming a nuclear transfer unit; and (iii) activating the nuclear transfer unit when the recipient oocyte is in metaphase II, thereby reconstituting an embryo; wherein said reconstituted embryo undergoes cell division directed by said treated nucleus.