Patent ID: 6991787

Claim:
A method for preserving transfected bone marrow stromal cells (BMSCs) comprising: (a) providing BMSCs which have been transfected with an exogenous gene (transfected BMSCs), the level of expression of the exogenous gene of the transfected BMSCs having a predetermined value; (b) washing said transfected BMSCs; (c) detaching said transfected BMSCs from a tissue culture dish; (d) suspending said transfected BMSCs in cryopreservation medium comprising: about 10% dimethyl sulfoxide, about 1–50% fetal bovine serum, and about 89–40% Dulbecco's modified Eagles' medium; (e) storing the transfected BMSCs at about −80° C.; wherein said transfected BMSCs in the thawed state have a level of expression of the exogenous gene which is at least about 77% of said predetermined value.