Patent ID: 7767411

Claim:
A process for determining whether a substance is a specific inhibitor of a protein kinase C-related kinase (PRK), wherein the process comprises selecting a substance to be tested for its PRK-related inhibitory activity; providing at least one plurality of identical reaction mixtures which comprise at least one PRK, androgen receptor (AR), and RhoA V14 and allowing the at least one PRK to exert at least one measurable effect in the identical reaction mixtures under physiological conditions, the at least one measurable effect comprising a RhoA V14 dependent activation of the AR by the PRK; combining each of the plurality of identical reaction mixtures with a different amount of the substance to be tested; determining the at least one measurable effect as a function of the concentration of the substance to be tested in the reaction mixture and establishing that the substance is a specific inhibitor of the at least one PRK if an inhibitory effect of the substance on the RhoA V14 dependent activation of the AR by the PRK is detected.