Patent ID: 8715936

Claim:
A method of determining a type of methicillin-resistant Staphylococcus aureus (MRSA) in a biological sample, comprising the steps of (a) providing a biological sample; (b) performing a multiplex PCR analysis of the biological sample, using (i) a first primer pair comprising a forward primer 5′UTR 3 (SEQ ID NO: 3) and a reverse primer mec124b (SEQ ID NO: 8), (ii) a second primer pair comprising a forward primer 5′UTR 3 (SEQ ID NO: 3) and a reverse primer mec3b (SEQ ID NO: 11), and (iii) third primer pair specific for SCCmec type IV, comprising a forward primer ccrAB-F1 (SEQ ID NO: 14) and a reverse primer ccrAB-R1 (SEQ ID NO: 18), wherein the PCR analysis provides a plurality of amplicons having different sizes; and (c) determining the PCR amplicons with respect to their different sizes so as to reveal if type I, type II, type III or type IV MRSA is present in the biological sample.