Patent ID: 6916619

Claim:
A method of mutation analysis of a target nucleic acid, said method comprising: incubating a sample comprising said target nucleic acid in a reaction mixture, in the presence of at least one first nucleic acid and at least one second nucleic acid, wherein said first nucleic acid comprises a primer sequence which anneals to a unique site of a sequence of SEQ ID NO. 1 or 2, said second nucleic acid has an opposite orientation from said first nucleic acid, said first or second nucleic acid comprises a sequence selected from the group consisting of SEQ ID NOs. 3-49; and wherein said incubation produces amplified products; denaturing said amplified products and re-generating duplexes in said reaction mixture; and detecting the presence or absence of a heteroduplex from said duplexes, wherein the presence of a heteroduplex indicates the presence of a potential mutation in said target nucleic acid, and wherein the absence of a heteroduplex indicates the absence of a mutation in said target nucleic acid.