Patent ID: 8889357

Claim:
A method for rapid detection of a target nucleic acid amplification product which prevents contamination of the target nucleic acid amplification products comprising the steps of: a) performing nucleic acid amplification in a fluid in an unopened reaction tube to produce the target nucleic acid amplification product; b) placing the unopened reaction tube inside a fixing case part; c) placing the fixing case part inside an open detection unit; d) closing the detection unit such that the reaction tube is punctured and a washing buffer vacuole containing washing buffer is punctured such that the fluid containing the target nucleic acid amplification product and the washing buffer is transferred to a sterile visual detection strip within the device such that the fluid is retained within the detection device; and (e) detecting the target nucleic acid amplification product, wherein the detection unit comprises an inner core ( 1 ) and an outer casing ( 15 ), wherein the inner core comprises a fixing case part ( 2 ); a base part ( 5 ), wherein the fixing case part ( 2 ) contains two holes respectively for a washing buffer vacuole ( 3 ) and a reaction tube ( 11 ) containing an amplification product ( 7 ) and wherein the base part ( 5 ) comprises a washing buffer container ( 21 ) having a vacuole puncture needle ( 4 ), an amplification product container ( 22 ) having a blade ( 9 ), a sealing diaphragm ( 6 ), a piece of glass fiber paper ( 8 ) and a test strip sealing part having a test strip ( 13 ) sealed therein and a transparent window ( 12 ); and the outer casing comprises a handle cover ( 16 ), a fixing case pressing part ( 17 ), a washing buffer vacuole extruding part ( 18 ), and a transparent window ( 20 ).