Patent ID: 8221995

Claim:
A method of formulating a CCL23 immunoassay for use in assessing a subject with SIRS, comprising: providing an antibody pair for use in a sandwich format immunoassay, wherein said antibody pair is selected from the group consisting of: (1) a first and a second antibody, each of which binds to an epitope common to both CCL23 and CCL23 splice variant; (2) a first antibody that binds to the portion of CCL23 missing N-terminal processed forms of CCL23 selected from the group consisting of CCL23 19-99 , CCL23 22-99 , CCL23 27-99 , and CCL23 30-99 , and a second antibody that binds to an epitope common to both CCL23 and CCL23 splice variant; (3) a first antibody that binds to CCL23 splice variant but not CCL23, and a second antibody that binds to an epitope common to both CCL23 and CCL23 splice variant; and (4) a first and a second antibody, each of which binds to CCL23 splice variant but not CCL23; measuring a sample obtained from a subject having SIRS using said antibody pair in a sandwich format immunoassay; and assessing said subject having SIRS based on said measuring.