Patent ID: 8895298

Claim:
A method of producing a cell population enriched in a first type of cell larger than an adult, enucleated red blood cell, said method comprising: continuously flowing a blood sample of a specific volume through a channel in a microfluidic device comprising; (i) continuously flowing the blood sample past a series of obstacles in the channel, the obstacles fixed in position separated by gaps arranged so that flow of the blood sample past the obstacles directs adult, enucleated red blood cells and cells smaller than adult, enucleated red blood cells in a first direction and directs cells larger than adult, enucleated red blood cells in a second direction to produce a first sample enriched in cells larger than adult, enucleated red blood cells; and without stopping the flow of the first sample, (ii) continuously flowing the first sample past obstacles that each comprise one or more binding moieties that preferentially bind to the first type of cell in the first sample, thereby producing a population enriched in the first cell type.