Patent ID: 7851217

Claim:
A process for fabricating a urinary bladder tissue, comprising the steps of: providing a collagen based acellular tissue membrane obtained from one or more of urinary bladder, urinary bladder submucosa, aorta, urethra, small intestinal submucosa or dermal matrix, the acellular tissue membrane having a first side and an opposing second side; collecting smooth muscle cells and interstitial fibroblastic cells from a urinary bladder; collecting urothelial cells from a urinary bladder; culturing smooth muscle cells and interstitial fibroblastic cells in two dimensions between two to four passages to confluence; culturing the urothelial cells in two dimensions to confluence in a plurality of passages; seeding the first side of the acellular tissue membrane with smooth muscle cells and interstitial fibroblastic cells from a selected passage; seeding the second side of the acellular tissue membrane with urothelial cells from a selected passage; and culturing the seeded acellular tissue membrane by simultaneous mechanical and biochemical stimulation; wherein the mechanical stimulation comprises applying tension cyclically to the seeded acellular tissue membrane, wherein the periodicity of the tension comprises a stretching period of about 10 seconds to about 20 seconds and a relaxing period of about 10 seconds, and the strain resulting therefrom is approximately 10%, and the mechanical stimulation continues until the acellular tissue membrane comprises confluently cultured cells; and the biochemical stimulation includes culturing the seeded acellular tissue membrane in a serum-conditioned culture medium, wherein the serum-conditioned culture medium comprises autologous serum or fetal bovine serum conditioned by proliferating urothelial cells, and wherein the serum-conditioned culture medium comprises about 5% serum for a first cultivation period, 1% serum for a second cultivation period, and about 0% serum for a third cultivation period; and resulting in a urinary bladder tissue comprising: at least one layer of smooth muscle cells and interstitial fibroblastic cells, wherein said layer comprises between 75% and 85% smooth muscle cells and between 15% and 25% fibroblastic cells; and at least one layer of urothelial cells bounded by the acellular tissue membrane.