Patent ID: 8791088

Claim:
A method of modulating splicing of a LMNA pre-mRNA in a cell, comprising contacting the cell with a single-stranded antisense oligomeric compound comprising an oligonucleotide consisting of 12 to 30 linked nucleosides, wherein the oligonucleotide has a nucleobase sequence comprising an at least 8 nucleobase portion complementary to an equal length portion of nucleotides 1983-2062 of SEQ ID NO: 4 and is at least 90% complementary to LMNA pre-mRNA having SEQ ID NO:4 as measured over the entirety of the oligonucleotide, wherein the oligonucleotide comprises at least one modified sugar moiety; and thereby modulating splicing of the LMNA pre-mRNA in the cell, wherein the modulation of splicing results in an increase in the ratio of full-length LMNA mRNA to truncated LMNA mRNA.