Patent ID: 8592226

Claim:
A method for the in vivo determination of glucose concentration, comprising: a) contacting an implanted biosensor with a bodily fluid to create a biosensor-glucose ensemble: i) wherein said bodily fluid comprises glucose, ii) wherein said implanted biosensor comprises, 1. a substrate, 2. nanospheres, said nanospheres in contact with said substrate, 3. a metal film, said metal film formed over said nanospheres, and 4. a self-assembled mixed partition layer, said self-assembled mixed partition layer adhered to said metal film, said self-assembled mixed partition layer comprising at least two discrete compounds, a. said two discrete compounds comprising decanethiol and mercaptohexanol, and iii) wherein said glucose concentrates within the self-assembled mixed partition layer in close physical proximity to the metal film; b) directly detecting said glucose in said biosensor-glucose ensemble with surface-enhanced Raman spectroscopy to create a surface-enhanced Raman signal; and c) correlating said surface-enhanced Raman signal to the concentration of said glucose in said bodily fluid.