Patent ID: 7807360

Claim:
A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber, the method comprising: introducing a nucleic acid-containing sample and a solution comprising a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the micro chamber, wherein the performing PCR comprises adding polyethylene glycol and bovine serum albumin (BSA) to the micro chamber, and wherein concentrations of the polyethylene glycol and bovine serum albumin (BSA) are 2.5-10% by volume and 0.1-10 mg/ml, respectively, and wherein the kosmotropic salt is at least one selected from the group consisting of phosphate (HPO 4 2− ), hydroxide (OH − ), fluoride (F − ), formate (HCOO − ), and sodium sulfate (Na 2 SO 4 ).