Patent ID: 7267971

Claim:
A process for preparing thermostable glucose oxidase of transition temperature (T m ) of about 80° C. that is useful for biological processes of high temperature, said process comprising: a. silanization of activated glass beads by evaporative deposition of aminopropyl triethoxysilane in acetone, at a concentration of aminopropyl triethoxysilane ranging between 1-8%, forming silanated glass beads; b. heating the silanated glass beads overnight at about 115° C. to obtain heated glass beads; c. treating the heated glass beads with glutaraldehyde in a phosphate buffer of pH about 6.0 for one hour with and one hour without vacuum to obtain treated beads; d. exposing the treated beads to glucose oxidase at about 30° C. and for a time duration ranging between 3-5 hours with intermittent shaking obtaining Schiff's base; e. reducing the Schiff's base obtained in (d) by treating the beads with sodium cyanoborohydride; f. obtaining thermostable glucose oxidase immobilized on the beads having a transition temperature (T m ) of about 80° C.; and g. blocking unoccupied regions of the glass beads with glycine.