Patent ID: 7238501

Claim:
A method for producing trans-4-hydroxy-L-proline, which comprises: (a) cultivating in a medium a transformant obtained by introducing into host cell selected from the group consisting of microorganism and isolated animal cell a recombinant DNA comprising a DNA coding for L-proline-4-hydroxylase, wherein the DNA is a DNA having any one of nucleotide sequences of SEQ ID NO: 3, 9, and 16, or is a DNA hybridizing to the DNA having any one of nucleotide sequences of SEQ ID NO: 3, 9 and 16 in a solution comprising 50% (v/v) formadide, 2% blocking reagent, 0.1% (w/v) N-laurylsarcosine, 0.02% (w/v) SDS, 750 mM sodium chloride and 75 mM sodium citrate at 42° C. for 1 hour, wherein the hybridizing comprises additional steps of: washing twice in a solution containing 0.1% SDS, 300 mM sodium chloride and 30 mM sodium citrate at room temperature for 5 minutes, and washing twice with a solution comprising 0.1% SDS, 15 mM sodium chloride and 1.5 mM sodium citrate at 68° C. for 15 minutes each; (b) producing and accumulating trans-4-hydroxy-L-proline; and (c) collecting the trans-4-hydroxy-L-proline from the resulting culture.