Patent ID: 7723075

Claim:
A method for screening for a candidate agent capable of modulating monovalent cation permeability of a Transient Receptor Potential Melastatin-like 5 (TRPM5) channel, wherein said TRPM5 channel comprises a polypeptide having the amino acid sequence according to SEQ ID NO: 2, the method comprising: a) providing a recombinant cell comprising a recombinant nucleic acid encoding said polypeptide and an inducible promoter operably linked thereto; b) inducing the recombinant cell to express the TRPM5 polypeptide and form a TRPM5 channel comprising the TRPM5 polypeptide; c) contacting the recombinant cell with a candidate agent; d) activating the TRPM5 channel; and e) detecting modulation of monovalent cation permeability of the TRPM5 channel.