Patent ID: 8003328

Claim:
A method of determining a nucleotide in a bovine subject at a position in which a single nucleotide polymorphism is correlated with a predicted occurrence of a trait comprising: detecting a target polynucleotide in a bovine sample with at least one oligonucleotide capable of binding the target polynucleotide, wherein the target polynucleotide comprises a nucleotide at a position of the prolactin promoter corresponding to a position of SEQ ID NO:14 selected from the group consisting of position 1167 or 1286; determining the nucleotide at the single nucleotide polymorphism in the bovine subject; and correlating a guanine or adenine at a position of the target polynucleotide corresponding to position 1167 of SEQ ID NO: 14 or a cytosine or a thymine at a position of the target polynucleotide corresponding to position 1286 of SEQ ID NO: 14 to the predicted occurrence of the trait, the trait selected from the group consisting of calf height at weaning, calf weight at weaning, calving rate, breed lineage, milk protein concentration, somatic cell counts in the milk, milk butterfat concentration and calf weight at birth.