Patent ID: 8105834

Claim:
A method of treating a urinary tract disease in a mammalian subject in need thereof comprising: (a) isolating skeletal muscle cells from a human, (b) cooling the cells to a temperature lower than 10 ° C. and storing the cells for 1-7 days; (c) suspending the human skeletal muscle cells in a first cell culture container for between 30 and 120 minutes; (d) decanting the medium from the first cell culture container into a second cell culture container; (e) allowing the remaining cells in the medium in the second container to attach to the walls of the second cell culture container; (f) isolating the cells from the walls of the second cell culture container, wherein the isolated cells are muscle derived progenitor cells (MDCs); (g) culturing the MDCs isolated in step (f) to expand their number; (h) freezing the MDCs prepared in step (g) to a temperature below −30 ° C.; and (i) thawing the MDCs prepared in step (h) and administering the thawed MDCs to the urinary tract of the mammalian subject; thereby, treating urinary tract disease in a mammalian subject in need thereof.