Patent ID: 7563591

Claim:
A method for assaying the activity of a lysosomal enzyme selected from the group consisting of α-L-iduronidase, β-D-galactosidase, β-D-glucosidase, α-D-galactosidase A, total hexosaminidase, hexosaminidase A, arylsulfatase B, sphingomyelinase and iduronate-2-sulfatase present in a dried blood spot, said method comprising: (a) placing blood on a porous surface material and drying to form a dried blood spot, (b) combining the dried blood spot with (1) an eluent, (2) an incubation buffer and (3) at least one substrate capable of reacting with said lysosomal enzyme and generating at least one enzyme product, to form an incubation media; (c) incubating said media under conditions sufficient to generate said enzyme product; and (d) measuring said enzyme product to determine the activity of said lysosomal enzyme.