Patent ID: 7112344

Claim:
A vapor fraction from seeds of Glycine max (L.) Merr., which vapor fraction is prepared by the process comprising the steps of: (a) providing a crude extract of the seeds in an alcohol solution containing alcohol at the concentration lower than about 15% wt. or in water; and (b) vaporizing the crude extract at a barometric pressure lower than about 1 atm and at a temperature lower than about 110° C. to obtain the vapor fraction; wherein a spectrogram of the 50 mL of the vapor fraction taken at 200 nm through a high performance liquid chromatography (HPLC) using Waters Sphersorb™-ODS2 column with an inner diameter (I.D.) of 4.6 mm, a length (L) of 250mm and a particle size of 5 mm comprises peaks at a retention time of 2.910, 5.190, 13.190, and 50.815 minutes with the coefficient of variation of 8%, where mobile phase is at 0 to 5 minutes is 95% H 2 O/5% CH 3 CN; at 5 to 30 minutes is 95% H 2 O/5% CH 3 CN in gradient, at 30 to 80 minutes is 75% H 2 O/25% CH 3 CN in gradient; at 80 to 100 minutes is 0% H 2 O/100% CH 3 CN in gradient; at 100 to 101 minutes is 0% H 2 O/100% CH 3 CN in gradient; and at 101 minute is 95% H 2 O/5% CH 3 CN and a flow rate is 1 mL/min.