Patent ID: 7514098

Claim:
A composition for in vivo gene delivery, comprising: a complex of nucleic acid and targeting cross-linked nanoparticles, wherein said targeting cross-linked nanoparticle comprises: cross-linked neutral amphipathic molecules (NAM) comprising a hydrophilic head group; a hydrophobic tail group; and a linker or covalent bond between the head and tail groups, and further comprising a crosslinking functional group providing for oligomer formation, cationic amphipathic molecules (CAM) comprising a hydrophilic head group; a hydrophobic tail group; and a linker or covalent bond between the head and tail groups, and is positively charged; and targeting amphipathic molecules (TAM) comprising a hydrophilic head group; a hydrophobic tail group; and a linker or covalent bond between the head and tail groups, and further comprising a covalently bound targeting moiety other than a non-peptidic a v b 3 integrin antagonist, in the proportions wherein said CAM comprise from about 5 to 40% by weight of the total amphipathic molecules, and said TAM comprises from about 1 to 20% by weight of the total amphipathic molecules; wherein each of said amphipathic molecules are lipid molecules, and wherein said complex is formed by crosslinking of said nanoparticles and following formation of said nanoparticles, mixing said nanoparticles with said nucleic acid.