Patent ID: 8859234

Claim:
A method for modifying the glycosylation of a polypeptide produced by a host cell, comprising introducing into said host cell a nucleic acid comprising a sequence encoding a fusion polypeptide, wherein said fusion polypeptide has β(1,4)-galactosyltransferase activity and comprises a Golgi localization domain selected from the group consisting of: the localization domain of mannosidase II, the localization domain of β(1,2)-N-acetylglucosaminyltransferase I, the localization domain of mannosidase I, the localization domain of β(1,2)-N-acetylglucosaminyltransferase II, and the localization domain of α1-6 core fucosyltransferase, and wherein said modified polypeptide has increased Fc-receptor binding or effector function as a result of said modification.