Patent ID: 6846632

Claim:
A method for rapidly identifying porcine estrogen receptor marker by a mutagenically separated polymerase chain reaction, the method comprising the steps of: (a) extracting a DNA sample from a pig to be identified; (b) proceeding with a polymerase chain reaction by means of combining the DNA sample with a solution comprising dNTP (deoxy-nucleotide-triphosphates), a first primer, a second primer, a third primer, magnesium chloride, buffer solution, and taq DNA polymerase, wherein the first primer has a DNA sequence as follows: 5′-GTCAGTCCAATTAGAATAGGGCGGGAATGGGGACTTG ACAAGAAACGT-3′; the second primer has a DNA sequence as follows: 5′-GTGGAATGGGACTTGACAAGAACACC-3′; and the third primer R1 has a DNA sequence as follows: 5′-CCTGTTTTACAGTGACTTTTACAGAGTATA-3′; and (c) electrophoresizing and dying a product resulting from step (b) to thereby identify the DNA sample by a band of the DNA sample, in which the DNA sample is identified as prolific genotype when having 90 bp band, the sample is identified as non-prolific genotype when having 110 bp band, and the sample is identified as hetero-genotype when having 90 bp bands and 110 bp bands.