Patent ID: 7045357

Claim:
A method for producing a fertile transgenic plant, comprising the steps of: (a) inoculating a regenerable plant cell or tissue selected from the group consisting of wheat immature embryos, maize immature embryos, wheat embryogenic callus, maize embryogenic callus, soybean hypocotyl sections and soybean callus suspension cell cultures with a solution comprising Agrobacterium containing a genetic component, said genetic component comprising a nucleic acid sequence of interest encoding a selectable or screenable marker that functions in the identification of a transformed plant cell or tissue, to produce an Agrobacterium -inoculated explant; (b) substantially removing said solution of Agrobacterium ; (c) co-culturing said Agrobacterium -inoculated explant in a vessel with about 100 to about 300 microliters of media, said media not containing a gelling agent wherein the weight of the Agrobacterium -inoculated explant is reduced from about 20% to about 35% by the end of the co-culture period; (d) identifying and selecting a transformed plant cell or tissue comprising said genetic component; and (e) regenerating a transgenic plant therefrom.