Patent ID: 8198084

Claim:
A method for producing a recombinant protein in vitro, comprising culturing a recombinant CHO cell in suspension, in a serum-free and protein-free culture medium comprising zinc at a final concentration of about 0.00007 to 0.00073 g/L, and iron at a final concentration of about 0.00028 to 0.011 g/L, under conditions suitable for supporting the cultivation of the CHO cell to a density of about 5×10 6 cells/ml, in said suspension and expression of said recombinant protein, the conditions comprising culturing the CHO cell at about 37 degrees Celsius in a humidified atmosphere containing about 3-20% carbon dioxide in air, and wherein said serum-free and protein-free culture medium supports increased levels of cell growth as compared to culture in FMX-8 medium.