Patent ID: 8357529

Claim:
A system for in vitro transcription of mRNA and/or translation of polypeptides in a reactor, comprising: a reaction mix comprising an extract from E. coli cells comprising membrane vesicles containing respiratory chain components; components of polypeptide and/or mRNA synthesis machinery; a template for transcription of said mRNA and/or translation of said polypeptide; monomers for synthesis of said mRNA and/or polypeptides; and co-factors, enzymes and other reagents necessary for said transcription and/or translation; magnesium at a concentration of from about 5 mM to about 20 mM; wherein said reaction mix is substantially free of polyethylene glycol; and oxidative phosphorylation, which is sensitive to electron transport chain inhibitors, is activated in said reaction mix; and head space of the reactor filled with oxygen.