Patent ID: 7601520

Claim:
A method for rapidly identifying a point mutation in porcine insulin-like-growth factor 2 intron 7 by a mutagenically separated polymerase chain reaction, the method comprising the steps of: (a) extracting a DNA sample from a pig to be identified, the DNA sample including insulin-like-growth factor 2 intron 7; (b) proceeding with a polymerase chain reaction by combining the DNA sample with a solution comprising: a first primer (C primer), wherein said C primer is the DNA sequence: 5′TGTCTGTCTCTCTGTTTCTCTCCCGAGGGTCTGAGACTTCATAC-3′ (SEQ ID NO: 3), a second primer (G primer), wherein said G primer is the DNA sequence: 5′-CTCCGAGGGTCTGAGACTTCAGAG-3′ (SEQ ID NO: 4), a third primer (K primer), wherein said R primer is the DNA sequence: 5′-CAGGCACATGGCAGGTGCCAATCAATG-3′ (SEQ ID NO: 5), and a reaction mixture required for polymerase chain reaction, the first primer amplifying a lean muscle mass-enhancing allele, the second primer amplifying a lean muscle mass-suppressing allele, and the third primer being the third primer is shared by the first primer and the second primer in the PCR reactions; and (c) electrophoresizing a product resulting from step (b) to thereby identify the DNA sample by a band of the DNA sample, in which the DNA sample is identified as muscular genotype when having 92 bp band, the sample is identified as non-muscular genotype when having 72 bp band, and the sample is identified as hetero-genotype when having both 92 bp and 72 bp bands.