Patent ID: 8818070

Claim:
A method of predicting hepatotoxicity of a compound, the method comprising: imaging cells of hepatic origin positioned within a plurality of containers to obtain imaged cellular targets, each container being treated with a different concentration of the compound, the imaging being performed using a quantitative high-content cell imaging system; quantitatively measuring the imaged cellular targets to detect changes in multiple cellular targets associated with cytotoxicity of the compound; and analyzing measurements obtained from the measured imaged cellular targets over a range of compound concentrations, comprising: determining data normalization values for each cellular target, comprising: determining a mean target value from measurements of vehicle control wells associated with the cellular target; determining normalized target response values for the compound at each compound concentration by dividing a measured response of the compound at the corresponding compound concentration by the mean target value; and determining normalized target response values for a negative control at each compound concentration by dividing the measured target response of the negative control at the corresponding compound concentration by the mean target value; determining toxicity thresholds for each cellular target; identifying the toxicity response for each cellular target; and predicting hepatotoxicity of the compound using multiparametric data.