Patent ID: 8637246

Claim:
A method of detecting a BRAF mutation in a subject having a cancer selected from: melanoma, colon carcinoma or thyroid carcinoma, the method comprising (a) obtaining a cancer cell-containing sample from the subject; and (b) performing a nucleic acid-based assay to analyze a nucleic acid molecule encoding a BRAF polypeptide for one or more mutations with respect to a nucleic acid molecule encoding a wild-type BRAF polypeptide (SEQ ID NO: 1) or a BRAF V600E polypeptide (SEQ ID NO: 3); and (c) detecting in the assay the one or more mutations occurring at positions encoding one or more amino acids in the BRAF polypeptide selected from the group consisting of A29, H72, SI 13, S124, P162, C194, L227, P231, C251, V291, Q329, V483, L485, T521, V528, D587, P655, S657, S683, P686, C696, L697, P722, F738, and C748.