Patent ID: 8143034

Claim:
A method for producing ricinoleic acid analogs comprising the steps of: a) cultivating a microorganism in a seed culture stage to produce an inoculum, wherein the microorganism is selected from the group consisting of microorganisms capable of producing P450 mono-oxygenase; b) transferring the inoculum into a fermentation medium; and c) maintaining growth stage conditions in a main fermentation stage to facilitate the catalysis of a substrate to ricinoleic acid analogs, wherein the main fermentation stage is maintained at a pH of between about 5.0 and 8.0 and a temperature of between about 22° C. and about 30° C. for a time period of between about 10-12 hours, wherein the resulting ricinoleic acid analog has a formula selected from the group consisting of 1,18-cis-9-octadecenedioic acid; 1,22-cis-9-docosenedioic acid; 1,18-cis-9,12-octadecadienedioic acid; 7-hydroxy-1,18-cis-9-octadecenedioic acid; 12,18-dihydroxy-cis-9-octadecenoic acid; cis-9,10-epoxy-1,18-octadecanedioic acid; 7-tetradecynedioic acid; and 8-hexadecynedioic acid.