Patent ID: 7579454

Claim:
A method for detecting and/or identifying Gram-positive bacteria with high GC content, which comprises: a) subjecting a sample composition to a nucleic acid amplification reaction, with there being a first primer sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, SEQ ID NO. 7 and SEQ ID NO. 9 and there being a second primer sequence selected from the group consisting of SEQ ID NO. 2, SEQ ID NO. 4, SEQ ID NO. 6, SEQ ID NO. 8 and SEQ ID NO. 10; b) hybridizing a composition comprising the amplification product of step a) or a part thereof with one or more oligonucleotide probe sequences selected from any one of SEQ ID NO. 11 to 42 or sequences complementary thereto; and c) determining the degree of hybridization by reference to a control sample.