Patent ID: 7868165

Claim:
A process for the isolation of low-molecular weight aminoglycan compounds of formula I from waste egg shells; wherein M may be at one or more instances Na, Ca, K, Mg; and n is an integer between 20 and 40, said process comprising the steps of: (a) pre-preparation of the waste egg-shells for extraction of the embryonic low molecular weight aminoglycan compound of formula I using a polar organic solvent in water, wherein the pretreated egg shells are mixed thoroughly with a polar organic solvent in water at temperatures between 25° C. and 40° C. for 1 hour to 4 hours, followed by decantation of the supernatant and the eggshells carried forward for extraction; (b) extraction of the low molecular weight aminoglycan compound of formula I as its water soluble salt using an aqueous polar salt solution, wherein the eggshells from step (a) are vigorously shaken with the aqueous polar salt solution at 25° C. to 40° C. for 6 to 24 hours, followed by decantation, filtration or centrifugation to collect the aqueous layer containing the dissolved aminoglycan compound of formula I; (c) isolation of the purified low molecular weight aminoglycan compound of formula I by gel formation out of the aqueous salt mixture by using a polar organic solvent, wherein the solution from step (b) is subjected to sequential step wise addition of the polar organic solvent in an amount between 75% and 150% volume/volume of the polar organic solvent between 10° C. and 20° C. in 1 hour to 2 hours and the gel formed is allowed to stand for 4 hours to 12 hours to complete precipitation, followed by decantation, filtration or centrifugation to isolate semi dried aminoglycan compound of formula I containing between 4% to 8% moisture; (d) stabilization of the isolated aminoglycan compound of formula I from step (c) by sequential introduction of organic oils into the semi-dried gel to form ordered sheets of aminoglycan compound of formula I.