Patent ID: 8475806

Claim:
A plurality of influenza virus vectors for high titer reassortant recombinant influenza virus production comprising: a plurality of vectors for vRNA production comprising: a vector comprising an influenza virus PA cDNA having SEQ ID NO: 1; a vector comprising an influenza virus PB1 cDNA having SEQ ID NO:2; a vector comprising an influenza virus PB2 cDNA having SEQ ID NO:3; a vector comprising an influenza virus NP cDNA having SEQ ID NO:4; a vector comprising an influenza virus M cDNA having SEQ ID NO:5; a vector comprising an influenza virus NS cDNA having SEQ ID NO:6; a vector comprising an influenza virus H5 HA cDNA; and a vector comprising an influenza virus NA cDNA that does not include SEQ ID NO:8, wherein each vector for vRNA production further comprises a RNA polymerase I promoter and a RNA polymerase I terminator operably linked to the influenza virus cDNA, and wherein the sequences in the vectors for vRNA production are those for a reassortant influenza virus; and a plurality of vectors for mRNA production comprising: a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PA; a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB1; a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB2, and a vector comprising a promoter operably linked to a DNA segment encoding influenza virus NP.