Patent ID: 7432080

Claim:
A fermentation method for maximum production of teicoplanin using Actinoplanes teichomyceticus BNG 2315 deposited to Korean Culture Center of Microorganism with accession number KCCM-10601 comprising the steps of: i) fermenting cells of Actinoplanes teichomyceticus BNG 2315 on production medium comprising glucose 15-25g/L, dextrin 40-80g/L, peptone 4-6g/L, rapeseed meal 16-20g/L, soybean flour 16-20g/L, MgSO 4 .7H 2 O 0.4-0.6g/L, CaCO 3 4-6g/L, and NaCl 1.0-1.4g/L on condition that aeration rate of the medium is 0.5-2.0 volume of air per volume of medium per minute; ii) removing the cells and other residue from the fermentation medium; and iii) separating and recovering teicoplanin from the fermentation medium of step (ii), wherein the cells of Actinoplanes teichomyceticus BNG 2315 used for fermentation are pre-cultured in the seed-culture medium comprising glucose 25-35g/L, yeast extract 2.0-3.0g/L, soybean flour 7.0-11.0g/L, rapeseed meal 7.0-11.0g/L, NaCl 1.0-1.4g/L, CaCl 2 0.08-0.12g/L, MgSO 4 .7H 2 O 0.4-0.6g/L, and CaCO 3 4-6g/L.