Patent ID: 8293501

Claim:
A method of selectively amplifying two or more target nucleic acids of a nucleic acid sample, said method comprising: (a) contacting said nucleic acid sample with primer pairs specific for two or more target nucleic acid sequences of interest to produce a first reaction mixture; (b) subjecting said first reaction mixture to template-dependent primer extension reaction conditions to produce an amplified composition comprising target amplicons; (c) circularizing said target amplicons in said amplified composition; wherein said circularizing comprises: (i) contacting said amplified composition under hybridization conditions with circularization template oligonucleotides specific for each of said target amplicons to produce circularization complexes; and (ii) contacting said circularization complexes with a circularizing agent to circularize said target amplicon; and (d) selecting for said circularized target amplicons in said amplified composition.