Patent ID: 8333936

Claim:
A reagent splitting/dispensing method based on a reagent dispensing nozzle that controls a split amount or a dispensation amount of a reagent by an operation fluid disposed in the reagent dispensing nozzle, the reagent splitting/dispensing method comprising: a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle; a first moving process of moving the reagent dispensing nozzle to a position above the reagent to be split; a second moving process of depositing the nozzle tip end in the reagent; an air layer adjusting process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and decreasing the occupation amount of the first air layer, between the first moving process and the second moving process; a reagent splitting process of decreasing the occupation amount of the operation fluid in the reagent dispensing nozzle and filling the reagent into the reagent dispensing nozzle from the nozzle tip end; a third moving process of evacuating the tip end of the reagent dispensing nozzle from the reagent; a reagent protecting process of disposing a second air layer between an interface of the split reagent and the nozzle tip end; a fourth moving process of moving the reagent dispensing nozzle to a reagent dispensation position, after the reagent protecting process; a reagent dispensing process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and ejecting the split reagent; an operation fluid protecting process of disposing the first air layer between the interface of the operation fluid and the nozzle tip end, after dispensing the reagent; and a fifth moving process of evacuating the reagent dispensing nozzle to a waiting position, in a state where the first air layer is disposed between the interface of the operation fluid and the nozzle tip end.