Patent ID: 7112428

Claim:
An isolated gene comprising a nucleotide sequence selected from: (a) a nucleotide sequence described in SEQ. ID. NO: 1, (b) a nucleotide sequence which encodes a protein having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity and hybridizes with the nucleotide sequence described in SEQ. ID. NO: 1 under stringent conditions, wherein the stringent conditions comprise hybridization using a DNA derived from a colony or plaque, or a filter to which a fragment of the DNA is fixed, in the presence of 0.7 to 1.0 M NaCl at 65° C., and then washing the filter with 0.1 to 2-fold of a SSC solution at 65° C., (c) a nucleotide sequence which encodes an amino acid sequence described in SEQ. ID. NO: 2, and (d) a nucleotide sequence which encodes an amino acid sequence having a chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase activity where the amino acid sequence is an amino acid sequence having 95% or more homology with the amino acid sequence described in SEQ. ID. NO: 2.