Patent ID: 8058005

Claim:
A qualitative method for determining one or more single nucleotide polymorphisms in one or more target nucleic acids comprising: (a) independently hybridizing over a temperature gradient one or more fluorescently tagged target amplicons to two or more target nucleic acid probes immobilized in independent areas on an upper surface of a substrate, wherein the sequences of the two or more target nucleic acid probes differ so as to represent one or more single nucleotide polymorphisms; (b) independently activating a fluorescence response from each of the one or more fluorescently tagged target amplicons hybridized to the two or more target nucleic acid probes immobilized in independent areas on the upper surface of the substrate using an evanescent wave of a predetermined wavelength; (c) independently detecting each fluorescence response; (d) independently analyzing each fluorescence response; (e) independently differentially hybridizing each of the one or more fluorescently tagged target amplicons hybridized to each of the two or more target nucleic acid probes to provide a melting curve for each of the one or more fluorescently tagged target amplicons hybridized to each of the two or more target nucleic acid probes; and (f) independently analyzing each melting curve, the T m of each melting curve, or the ratio of the fluorescence of the two or more target nucleic acid probes at a selected temperature to qualitatively determine whether the one or more target nucleic acid sequence has one or more single nucleotide polymorphisms, wherein the analyzing each melting curve comprises a comparison of hybridization signal at a single temperature, a comparison of hybridization signal over a temperature range, or a detection of a melting temperature (T m ) change using a melting curve.