Patent ID: 8580539

Claim:
A method of determining an identity of a nucleotide in a template nucleic acid sequence, said method comprising: a. providing said template nucleic acid sequence complexed with a polymerase enzyme capable of template dependent synthesis of a complementary nascent sequence as a first complex; b. contacting said first complex with a labeled phospholink nucleotide, wherein said labeled phospholink nucleotide comprises a bead comprising at least one fluorophore, wherein said bead is encapsulated in an organic, inorganic, or polymeric material, and wherein said labeled phospholink nucleotide is complementary to a known nucleotide; and c. detecting whether said labeled phospholink nucleotide is incorporated into said nascent sequence, wherein incorporation of said labeled phospholink nucleotide is indicative that said complementary nucleotide is in a position in the template nucleic acid that is being processed by the polymerase enzyme.