Patent ID: 8361705

Claim:
A method comprising steps of: providing a glycan preparation; splitting the glycan preparation into two or more portions; subjecting a first portion of the glycan preparation to a first exoglycosidase procedure, wherein the first exoglycosidase procedure comprises sequential treatment of the first portion with at least two exoglycosidases, wherein the first portion is exposed to each exoglycosidase one at a time; subjecting a second portion of the glycan preparation to a second exoglycosidase procedure, wherein the second exoglycosidase procedure comprises simultaneous treatment of the second portion with the at least two exoglycosidases, wherein the second portion is exposed to each exoglycosidase at the same time; characterizing the cleavage products of the first and second exoglycosidase procedure; and comparing the characterization of the cleavage products of the first exoglycosidase procedure to the characterization cleavage products of the second exoglycosidase procedure so that at least one glycosylation feature of the glycan preparation is determined.