Patent ID: 7054000

Claim:
A method of calibration of magnification of a microscope with the use of a diffraction grating, comprising the steps of determining a mean period of a diffraction grating by irradiating the diffraction grating with an electromagnetic radiation having a known wavelength and analyzing a resulting diffraction pattern; determining a scatter of individual values of a period of the diffraction grating by multiple measurements of periods of the diffraction grating by a microscope in pixels in one area in a microscope field of view; calculating a mean value of the period and the scatter based on the measurements; determining a sufficient number of measurements of the period for providing an accepted statistic error of a magnification of the microscope; performing measurements corresponding to the determined acceptable number of measurements, of individual values of the period in pixels in a plurality of portions of the diffraction grating; calculating a general mean value of the period in pixels based on an immediately preceding step; and finally calculating a parameter corresponding to the magnification of the microscope based on the determined mean value of the period of the diffraction grating.