Patent ID: 8691206

Claim:
A method of treating a liver disorder, comprising: selecting an amount of horseradish peroxidase (HRP) and an amount of hydrogen peroxide (H 2 O 2 ); and mixing a polymer comprising a crosslinkable phenol group, said amount of HRP and said amount of H 2 O 2 in a solution, to crosslink said polymer to form said hydrogel, said solution further comprising a drug or a protein or both, injecting said solution to a gelation site in a subject in need of treatment of a liver disorder, wherein said amount of HRP and said amount of H 2 O 2 are selected to independently control a gelation rate in said solution and a crosslinking density in said hydrogel, with a constraint that the H 2 O 2 molarity in said solution is within a pre-determined range and the HRP concentration in said solution is above a pre-determined threshold, said pre-determined range and threshold having been determined on the basis that, if said H 2 O 2 molarity in said solution is limited to be within said range and said HRP concentration in said solution is limited to be above said threshold, said gelation rate in said solution would be substantially unaffected by variation in the selection of said H 2 O 2 molarity within said range, and said crosslinking density in said hydrogel would be substantially unaffected by variation in the selection of said HRP concentration above said threshold.