Patent ID: 8465989

Claim:
A system for analysis of a population of molecules, comprising: a vessel adapted to contain a plurality of labeled probe molecules, each selectively binds to at least one target molecule, in contact with a population of molecules potentially containing a target comprising said at least one target molecule so that at least one of said plurality of labeled probe molecules being bound to at least a portion of said target; a fluorescent excitation source adapted to direct an excitation beam through the vessel, the beam configured to cause at least some of said at least one labeled probe molecule to emit a probe specific signal; a detector adapted to detect said probe specific signal and produce a detection output accordingly; a signal modulator configured to perform a preferential modulation to at least one of the probe specific signal and said detection output, wherein the preferential modulation is configured to apply power at a level suitable for frequency modulation sufficient for moving said at least one bound probe molecule out of the excitation beam; and an analysis module adapted to analyze the detection output in consideration of said preferential modulation.