Patent ID: 7345759

Claim:
A method for measuring chemical and/or biological samples by means of spectroscopic or microscopic methods, wherein: the sample including at least one marker in a measuring volume is irradiated with electromagnetic radiation of at least two different wavelength ranges and/or polarizations, the excited marker emits radiation in an emission wavelength range, and the emitted radiation is detected by at least one detector, characterized in that the electromagnetic radiation used to excite the marker is pulsed in at least two different wavelength ranges and/or polarizations and the radiation pulses of the individual wavelength ranges and/or polarizations impinge on the sample with a temporal offset wherein a relative movement between the measuring volume and the marker, occurs in a relative movement period and the electromagnetic radiation used to excite the marker is pulsed in at least one wavelength range and/or one polarization such that at least two radiation pulses hit the marker within the relative movement period in which the measuring volume contains the marker.