Patent ID: 8663956

Claim:
A recombinant host cell comprising a heterologous polynucleotide that encodes a carbonic anhydrase, wherein the heterologous polynucleotide: (a) encodes a carbonic anhydrase having at least 95% sequence identity to SEQ ID NO: 55; (b) hybridizes under high stringency conditions with (i) the full-length complementary strand of SEQ ID NO: 54, or (ii) the full-length complementary strand of the cDNA sequence of SEQ ID NO: 54; or (c) has at least 95% sequence identity to SEQ ID NO: 54 or the cDNA sequence of SEQ ID NO: 54; wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and wherein the host cell is capable of producing a greater amount of C4-dicarboxylic acid compared to the host cell without the heterologous polynucleotide when cultivated under the same conditions.