Patent ID: 7504219

Claim:
A method for amplification of DNA, comprising the steps of: providing a reaction mixture cocktail including DNA to be amplified, a polymerase enzyme, nucleosides, and primers; providing a sonic energy source suitable for dissociating said DNA to be amplified from its double stranded form to single strands of complementary DNA; operating the sonic energy source in order to effect dissociation of double stranded DNA in the mixture into single strands of DNA without raising the temperature of said reaction mixture cocktail to a temperature which would effect thermal denaturation of the double stranded DNA; discontinuing operation of the sonic energy source in order to allow formation of double stranded DNA from the single strands of DNA without including a cooling step in order to allow formation of double stranded DNA; repeating operation of the sonic energy source and discontinuance of operation of the sonic energy source as many times as required to produce a desired amount of DNA.