Patent ID: 7829317

Claim:
A method of attaching biomolecule to surface of substrate, the method comprising: providing composition comprising polymer; the polymer comprising: at least about 40 mol-% N-substituted acrylamide, N,N-disubstituted acrylamide, N-substituted methacrylamide, N,N-disubstituted methacrylamide, or mixture thereof in an amount effective to provide a contact angle of about 30° to about 60° for a sessile water drop; and one or more pendent reactive groups configured to form covalent bonds with corresponding functional groups on biomolecule; wherein the one or more pendent reactive groups are selected from the group consisting of thermochemically reactive groups, photoreactive groups, or mixtures thereof; the polymer being configured to be covalently attached to the surface coating and immobilizing the composition on the substrate surface; providing solution comprising biomolecule comprising one or more functional groups reactive with the reactive groups; applying an aliquot of the solution to the substrate surface; and forming covalent bonds between the reactive group and the functional group of the biomolecule.