Patent ID: 7790439

Claim:
A biosensor comprising a developing layer for developing a sample solution containing an analyte, said developing layer comprising: a plurality of reagent immobilization parts, each part having one reagent immobilized thereto; a marker reagent part on which a marker reagent is held in its dry state, and the marker reagent is dissolvable by developing the sample solution; a sample solution applying part to which the sample solution is applied, and the biosensor having a fine space which is formed by a space formation member comprising a liquid impermeable material and covers the entirety of an end portion of the developing layer in the sample solution applying part; wherein said fine space which has a cell component contraction reagent and is connected to said developing layer and is a space into which a predetermined volume of the sample solution flows by capillary phenomenon to be mixed with the cell component contraction reagent when the sample solution is in contact with said fine space, the reagents on each part of the reagent immobilization parts are different from each other, and have different affinities for the analyte in the sample solution, and the sample solution, when it moves on the developing layer, comes in contact with the respective parts included in the developing layer, successively, in order of the sample solution applying part, the marker reagent part, and the plural reagent immobilization parts.