Patent ID: 7972786

Claim:
A kit for performing an assay comprising more than one primer pair, more than one detection probe, and a low copy number synthetic amplicon corresponding to each of the primer pairs, wherein the primer pairs are selected from the group consisting of: a) CAGCACCGTCTGGCCAAGAC (SEQ ID NO: 5), and GCAATAACTGATTGGTCAGG (SEQ ID NO: 6); b) CGTTGTATGACCAGAGATCTATTTTAGTGTCCT (SEQ ID NO: 7), and CCATCAGATTGAAAAAGAATTCT (SEQ ID NO: 8); c) CAGGAGGTCTATATTTGGTTCCATTGGC (SEQ ID NO: 9), and CGGTGGATTAAACAAAAGCA (SEQ ID NO: 10); d) CCCAATACAGGGGACATCACATTTCTTG (SEQ ID NO: 11), and CATGGGCTGACAGTGAT (SEQ ID NO: 12); e) GGTGACAGGATTGGTCTTGTCTTTAGC (SEQ ID NO: 13), and CTAACCGAGGTCGAAAC (SEQ ID NO: 14) f) GATGCAGCTTTTGCCTTCAACAGAG (SEQ ID NO: 15), and GGTCCAACCCTAATTCCAA (SEQ ID NO: 16); and g) CCTCCCTCTATAAAACCTGCTATAGCTCCAAA (SEQ ID NO: 17), and CGACTGGGCTCAGAAA (SEQ ID NO: 18); wherein the detection probe is a molecular beacon probe selected from the group consisting of: a) Texas Red-CGCGACTAGGGAACTCGCTCGCG (SEQ ID NO: 19)-Dabsyl, b) CY3-CGCGGATTGGCTTTTTACTTTCTCACCGCG (SEQ ID NO: 20)-Dabsyl, c) FAM- GGCGGATGCTGCTCCCACTACCGCC (SEQ ID NO: 21)-Dabsyl, d) CY5-CGCTGAAAGCGTTTCTCGAGGTCCTG (SEQ ID NO: 22)-BHQ1, e) Texas Red-GCGAGTTTGCATGTTCTCCTGTCTCGC (SEQ ID NO: 23)-Dabsyl, f) CY5-GCGAGTTTGCATGTTCTCCTGTCTCGC (SEQ ID NO: 23)-Dabsyl, g) CY3-GCGCTATAGAGAGAACAGCGC (SEQ ID NO: 25)-Dabsyl, Tm=33.8 h) FAM-GGCCGCCTATTACCTCTCGGCC (SEQ ID NO: 26)-Dabsyl, and i) CY5-CGCTGAAAGCGTTTCTCGAGGTCCTG (SEQ ID NO: 22)-BHQ1.