Patent ID: 8871496

Claim:
A microfluidic device for detection of an active enzymatic agent, the device comprising: a substrate defining at least a portion of a first channel, a second channel, and a third channel coupled between the first channel and the second channel; a first membrane positioned in the first channel and configured to block a substrate molecule including a reporter moiety and a sequence configured to be cleaved by the active enzymatic agent; at least one reagent loading channel coupled to the first channel, the third channel coupled to the first channel at a location between the reagent loading channel and the first membrane; a second membrane positioned in the second channel and configured to concentrate reaction products generated following exposure of the active enzymatic agent to the substrate molecule; and wherein the third channel is configured to transport reaction products from a vicinity of the first membrane to a vicinity of the second membrane; and a separation channel including a separation gel, the separation channel coupled to the second channel and configured for separation of the reaction products, an elution time of the reporter moiety to a detection region in the separation channel being indicative of presence or absence of the active enzymatic agent in the sample.