Patent ID: 7608420

Claim:
A process for the recovery of staurosporine of formula: from a fermentation broth comprising the steps of: i) adding tetrahydrofuran to the fermentation broth; ii) ultrafiltering the dilute fermentation broth obtained in step i); iii) optionally diafiltrating the retenate obtained in step ii) with a mixture of a water and tetrahydrofuran, and optionally combining the permeates of the ultrafiltration and the diafiltration; iv) optionally adjusting the pH of the permeate obtained in step ii) or step iii) to at least 8.5; v) concentrating the permeate; vi) adjusting the pH of the concentrate obtained in step v) to at least pH 8.5, unless the pH of said concentrate is already at least 8.5; and vii) collecting the precipitated staurosporine obtained in step vi).