Patent ID: 7772012

Claim:
A device for the detection of hemoglobin in a biological sample, comprising a carrier matrix which includes: (i) a sample application region for receipt of said biological sample; (ii) a first substrate region in liquid-conductive communication with, or combined with, the sample application region and having a pseudoperoxidase substrate loaded therein in a dried state; said pseudoperoxidase substrate comprising a peroxide or hydroperoxide reagent; and (iii) a first indicator region in liquid-conductive communication with, or combined with, the first substrate region and having an indicator applied thereto or impregnated therein, said indicator capable of producing a detectable response in the presence of heme and said pseudoperoxidase substrate; (iv) a second substrate region in liquid-conductive communication with the sample application region and having a detectable anti-globin immunointeractive molecule applied thereto or impregnated therein, said immunointeractive molecule forming a detectable globin-antiglobin complex in the presence of globin; and (v) a second indicator region in liquid-conductive communication with the second substrate region and having an anti-globin immunointeractive molecule immobilized therein, said immobilized immunointeractive molecule immobilizing said detectable globin-antiglobin complex.