Patent ID: 7576855

Claim:
A spectrophotometric method for a small volume of sample solution, comprising the steps of: providing a sampler including a flat plate member formed with at least one sample-holding hole which penetrates the flat plate member and has a sectional area simply decreasing in a thickness direction from a first surface to a second surface of the flat plate member; holding a target sample solution in said at least one sample-holding hole of said sampler positioned approximately horizontally while orienting said first surface in an upward direction and wherein said sample solution is held in said at least one sample-holding hole based on a surface tension of said sample solution, and said surface tension prevents said sample solution from flowing through said at least one sample-holding hole during analysis; emitting a measurement light to said held sample solution from above or below said flat plate member; and analyzing a transmitted light passing through said sample solution; wherein said step of analyzing includes correcting a variation in light path length due to fluctuation in volume of a sample solution held in said at least one sample-holding hole.