Patent ID: 7217516

Claim:
Method for analysing a nucleic acid sequence, the method at least comprising the steps of: (a) amplifying a restriction fragment generated from the nucleic acid to be analysed, in which the restriction fragment has been ligated to an adapter, and whereby a RAMP-primer and an AFLP-primer are used to amplify the nucleic acid sequence; wherein said RAMP primer comprises a 3′-part complementary to a compound microsatellite repeat and a 5′-part complementary to the nucleotides directly adjacent to the compound microsatellite repeat, said 5′-part consisting of 4–10 non-degenerate nucleotides; and wherein said AFLP primer is complementary to said adapter and optionally contains at its 3′ end between 1 and 6 selective nucleotides; and optionally comprising the further step of: (b) detecting the amplified nucleic acid sequences thus obtained.