Patent ID: 8367354

Claim:
A method for determining the level of TGF-β1 in a sample of powdered nutritional product, the method comprising: a. reconstituting the sample to a concentration of from about 160 mg/mL to about 170 mg/mL; b. avoiding a centrifuging step; c. activating the sample by adding an acid and a base in a ratio of sample:acid:base of about 1:0.2:0.2; d. diluting the sample using a buffer solution; e. determining the concentration of TGF-β1 in the sample comprising the steps: i. adding an antibody specific for TGF-β1 to at least one well of a microplate; ii. adding the diluted sample of step(d) to the at least one well of the microplate; iii. adding at least one detection antibody to the at least one well of the microplate to form a complex with any TGF-β1 in the sample; iv. adding at least one enzymatic substrate to the at least one well of the microplate to produce a visible signal; v. adding a detection reagent to the at least one well of the microplate; vi. detecting the visible signal with a microplate reader to determine the amount of TGF-β1 in the sample.