Patent ID: 7732183

Claim:
A method for producing an active recombinant human Beta-site APP cleaving enzyme (BACE) polypeptide comprising: a) expressing a polynucleotide sequence encoding a BACE polypeptide lacking all or a portion of the sequence corresponding to residues 1-66 of SEQ ID NO: 1, b) solubilizing the BACE polypeptide in a denaturant at a pH from about 10 to about 11 and in the presence of a reducing agent; c) diluting the solubilized BACE polypeptide in an aqueous solution having a temperature of about 1° C. to about 15° C., to obtain a diluted sample; and d) incubating the diluted sample at a temperature of about 4° C. to about 15° C. until the BACE polypeptide folds into an active enzyme.