Patent ID: 8592566

Claim:
An artificial immunostimulatory oligonucleotide comprising: the nucleotide sequence represented by the formula: 5′-(G) M PXCGYQ(G) N -3′ (SEQ ID NO.: 117) wherein, C is cytosine; G is guanine; X and Y are mutually independent and each represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive guanine residues, and the entire length of X+Y is 6 to 20 nucleotides; XCGY contains a palindrome sequence having a length of at least 8 nucleotides and XCGY has a length of 8 to 22 nucleotides; P and Q are mutually independent and each represent one nucleotide other than guanine, M represents an integer of 6 to 10, N represents an integer of 0 to 3; and the length of X and Y may be the same or different, and wherein the length of the artificial immunostimulatory oligonucleotide is 16 to 37 nucleotides in total, wherein the palindrome sequence contains CGATCG (SEQ ID NO: 59), ATCGAT (SEQ ID NO: 60), or GACGTC (SEQ ID NO: 61), but the artificial immunostimulatory nucleotide is not SEQ ID NO:5 GGGGGGTGCCGATCGGCAGGG.