Patent ID: 6908903

Claim:
A method of delivering an active agent to a targeted in vivo site comprising: (a) administering to a recipient a first conjugate comprising a targeting moiety and a member of a ligand/anti-ligand binding pair; (b) thereafter administering to the recipient a clearing agent capable of directing the clearance of circulating first conjugate; and (c) subsequently administering to the recipient a second conjugate comprising an active agent and a ligand/anti-ligand binding pair member, wherein the second conjugate binding pair member is complementary to that of the first conjugate; wherein the clearing agent comprises: (i) a hepatic clearance directing moiety comprising an iterative, two branch chemical framework to which a plurality of hexose residues are bound, wherein the plurality of hexose residues are a plurality of 1-desoxy-1-thio-N-acetylgalactosamine hexose residues; and (ii) a binding moiety directly or indirectly attached to the hepatic clearance directing moiety, wherein the binding moiety binds in vivo a compound to be cleared, wherein the clearing agent is characterized by a molecular mass between about 2,000 and about 20,000 daltons, and directs clearance of the compound by a hepatic pathway.