Patent ID: 7282331

Claim:
A method for the analysis of a target nucleotide sequence of interest in a sample, said method comprising: a. contacting the sample with at least one pair of probes (Probe A and Probe B), b. hybridizing Probe A and Probe B to the sample under hybridization conditions in which the desired degree of discrimination is achieved for an accurate result, wherein: i) Probe A comprises a nucleotide sequence which hybridizes under said hybridization conditions to both a target nucleotide sequence of interest and to a nucleotide sequence not of interest, and is labeled with a first fluorophore; ii) Probe B comprises a nucleotide sequence which hybridizes under said hybridization conditions to a nucleotide sequence not of interest adjacent the nucleotide sequence not of interest to which Probe A hybridizes, and is labeled with a quencher; c. detecting the hybridization of Probe A, wherein the fluorescence generated by the hybridization of Probe A to the nucleotide sequence not of interest is quenched by hybridization of probe B to the nucleotide sequence not of interest, and d. correlating the presence or amount of target nucleotide sequence of interest in the sample with the fluorescence generated upon hybridization of Probe A to the target nucleotide sequence of interest, wherein detection of the fluorescence of the fluorophore of Probe A is an indication of the presence or amount of the target nucleotide sequence of interest in said sample.