Patent ID: 8648170

Claim:
A method for culturing pluripotent cells, the method comprising the step of: culturing pluripotent cells on an insoluble substrate having a chemically-defined surface that presents a GAG-binding peptide in a chemically defined culture medium comprising a Rho-associated kinase (ROCK) inhibitor, wherein the GAG-binding peptide is SEQ ID NO:1 or SEQ ID NO:2, and wherein the ROCK inhibitor is selected from the group consisting of (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinolinyl)sulfonyl]homopiperazine dihydrochloride (H-1152), 1-(5-isoquinolinesulfonyl)piperazine hydrochloride (HA-100), 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), 1-(5-isoquinolinesulfonyl)-3-methylpiperazine (iso H-7), N-2-(methylamino) ethyl-5-isoquinoline-sulfonamide dihydrochloride (H-8), N-(2-aminoethyl)-5-isoquinolinesulphonamide dihydrochloride (H-9), N-[2-p-bromo-cinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H-89), N-(2-guanidinoethyl)-5-isoquinolinesulfonamide hydrochloride (HA-1004), 1-(5-isoquinolinesulfonyl)homopiperazine dihydrochloride (HA-1077), (S)-(+)-2-Methyl-4-glycyl-1-(4-methylisoquinolinyl-5-sulfonyl)homopiperazine dihydrochloride (glycyl H-1152) and (+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride (Y-27632).