Patent ID: 6878541

Claim:
A method for treating a patient with a papillomavirus pseudo-virus including plasmids, said method comprising: making a papillomavirus pseudo-virus by disrupting papillomavirus-VLPs by mixing said papillomavirus-VLPs with a disruption buffer, said disruption buffer including EGTA 20mM, DTT 40 mM, NaCl 300mM, and Tris-hydrochloric acid (Tris-HCl) (pH 8.0) 100mM, incubating the mixture for 60 minutes at room temperature, mixing said disrupted papillomavirus-VLPs with said plasmids by adding said plasmids to the mixture in 1/10 ratio, said plasmids being at a concentration 0.5-1.0 microgram/microliter, adding a stop buffer gradually to reassemble said papillomavirus-VLPs and said plasmids into pseudo-viruses of VLPs with said plasmids inside, said stop buffer including CaCl, 25mM and DMSO 20% by volume, and incubating the mixture at 4 centigrade from 4 to 12 hours; and administering said pseudo-virus via the mucosa of said patient to induce a mucosal response.