Patent ID: 7118871

Claim:
A molecule comprising the formula (1) shown below wherein: Nuc represents a nucleobase selected from the group consisting of adenine, cytosine, guanine, thymidine, uracil and analogs thereof; N represents a number from 1 through 8 indicating the number of phosphate moieties present in said molecule; X 1 and X 2 are independently selected from the group H, OH, NH 2 , O-alkyl, N-alkyl, S-Alkyl, P-alkyl, a halogen, an alkyl, a cyclo-alkyl, and an aromatic containing group, wherein the alkyl and cycloalkyl possess varying degrees of unsaturation and are optionally substituted; and F and Q comprise a pair of indicators, wherein cleavage of the molecule results in separation of the indicators which, in turn, yields a change in a measurable signal, further wherein F is not a quencher of Q's fluoresence.