Patent ID: 7989663

Claim:
A lysophospholipase D (LysoPLD) substrate comprising a lysophosphosphatidic acid derivative labeled with a fluorescent group and a quencher which is represented by the formula: wherein A represents O or S; B represents H, Na + , K + , Li + or an alkyl group with 1-20 carbon atoms; E represents O, CH 2 , CHF or CF 2 ; G represents H, OH, F, O-acyl or O-alkyl with 1-20 carbon atoms; K 1 and K 2 each represent H, F, alkyl with 1 to 20 carbon atoms, OH or CO 2 H; W represents C(O), NH(CH 2 ) m , [NHC(O)(CH 2 ) n ] p C(O) or NH(CH 2 CH 2 O) q C(O); X represents NH, N(CH 2 ) 2 or CH 2 ; Y represents O, S or NH; and Z represents C(O), (CH 2 ) m NH, [C(O)(CH 2 ) n ] p C(O)NH or (CH 2 CH 2 O) q C(O)NH; wherein m is an integer from 1 to 10; n is an integer from 1 to 12; p is an integer from 1 to 20; and q is an integer from 1 to 33; and wherein R 1 and R 2 are a fluorescent group or a quencher group, respectively.