Patent ID: 7906332

Claim:
A method for constructing a basement membrane specimen wherein the method comprises the following steps: (a) culturing cells having an ability to form a basement membrane on a support structure coated with a sugar-chain coat which can localize a receptor having an activity to accumulate a basement membrane component on the basal surface of the cells having an ability to form a basement membrane, wherein the sugar chain coat has a β-D-glucopyranosyl non-reducing end or a 2-acetoamide-2-deoxy-β-D-glucopyranosyl non-reducing end and, wherein the cells are adhered onto the support structure through the basement membrane; (b) removing cells from the basement membrane on the support structure by subjecting the cells adhered onto the support structure through the basement membrane to a delipidating treatment using a solvent having the ability to lyse lipid of the cells; (c) conducting a treatment to remove proteinous and nucleic acid residues contained by the cells using an alkaline solution after or at the same time as the delipidating treatment, whereby the cells are removed and the basement membrane is exposed on the support structure.