Patent ID: 6989162

Claim:
A process of isolation of acteoside of high hepatoprotection from the plant Colebrookea coppositifolia , said process comprising steps of: a. drying aerial parts of the plant, b. grounding the dried parts into powder, c. percolating the powder with water or ethanol for 3-4 times to obtain an extract, d. filtering the extract for clearing of suspended particles to obtain supernatant, e. drying the supernatant at about 45 to 55° C. to obtain a residue, f. fractionating the residue with chloroform, ethyl acetate, and butanol successively, g. subjecting the butanol fraction to adsorption chromatography of SiO 2 after adding methanol to the fraction, h. charging the adsorbed fraction to a glass column, i. eluting the column with solvents of increasing polarity of methanol:chloroform to obtain further fractions and repeating the process one more time, j. subjecting the fractions to column chromatography to obtain additional fractions, k. concentrating the additional fractions under reduced pressure to obtain acteoside as a residue.