Patent ID: 8735084

Claim:
A method for forming a biochip with a three-dimensional structure, comprising: providing a precursor solution comprising an ionic liquid, a hydrolysis catalyst, and at least one alkoxide monomer and/or aryloxide monomer, where the hydrolysis catalyst comprises one selected from the group consisting of the following or any combination of the following: acidic compound, and alkaline compound, and the precursor solution is formed by blendin the at least one alkoxide monomer and/or aryloxide monomer and the ionic liquid together to form a first mixture, adding the acidic compound to the first mixture to form a second mixture, and adding the alkaline compound to the second mixture to form the precursor solution; performing a blending process for the precursor solution to hydrolyze and polymerize the at least one alkoxide monomer and/or aryloxide monomer until the viscosity of the precursor solution reaches a specific viscosity more than or equal to 150 cps; setting the precursor solution to have the at least one alkoxide monomer and/or aryloxide monomer continue to undergo hydrolysis and condensation, so as to form a aerogel; performing an extracting process by a solvent for the aerogel to substitute the ionic liquid in pores of the aerogel; performing a freeze-drying process to remove the solvent in pores of the aerogel; performing a grinding process to grind the aerogel to form a aerogel powder; performing a modification process to introduce a specific moiety to the internal and external surfaces of the aerogel powder by mixing the aerogel powder with a modifier to form a modified aerogel powder; performing a coating process to coat and stabilize the modified aerogel powder on a specific region of a substrate, so as to form a biochip with a three-dimensional structure; and performing a converting process by providing a converter that comprises a first moiety and a second moiety and bonding the specific moiety of the aerogel powder with the first moiety of the converter to form a biochip having the second moiety on its surface; wherein the ionic liquid is formed by mixing an organic base with a Lewis acid that is not halogenated acid; the cationic moiety of organic base is selected from the group consisting of the following: 1-n-butyl-3-methylimidazolium (BMI) , 1-octanyl-3-methylimidazolium (DMI), and 1-hexadecanyl-3-methylimidazolium (HDMI); the anionic moiety in the Lewis acid is selected from the group consisting of the following: BF 4 − , PF 6 − , AsF 6 − , SbF 6 − , F(HF) n − , CF 3 SO 3 − , CF 3 CF 2 CF 2 CF 2 SO 3 − , (CF 3 SO 2 ) 2 N − , (CF 3 SO 2 ) 3 C − , CF 3 COO − , and CF 3 CF 2 CF 2 COO − ; and the weight of the ionic liquid is about 50%˜70% weight of the at least one alkoxide monomer and/or aryloxide monomer.