Patent ID: 8097438

Claim:
A method of producing 8-hydroxy-delta cadinene in a genetically modified host cell, the method comprising: culturing the genetically modified host cell in a suitable medium, wherein said host cell is genetically modified with: a) a nucleic acid comprising a nucleotide sequence encoding cytochrome P450 enzyme wherein the cytochrome P450 enzyme is δ-cadinene-8-hydroxylase operably linked to a heterologous domain selected from a heterologous transmembrane domain, a heterologous secretion domain, a heterologous solubilization domain, and a heterologous membrane-inserting protein, to produce an enzymatically active, modified δ-cadinene-8-hydroxylase; b) a nucleic acid comprising a nucleotide sequence encoding a cytochrome P450 reductase comprising the amino acid sequence set forth in SEQ ID NO:37; c) a nucleic acid comprising a nucleotide sequence encoding a cadinene synthase; and d) one or more heterologous nucleic acids comprising nucleotide sequences encoding mevalonate pathway enzymes, wherein the mevalonate pathway enzymes include: i) a mevalonate kinase; ii) a phosphomevalonate kinase; and iii) a mevalonate pyrophosphate decarboxylase, wherein production of said enzymes in said genetically modified host cell results in production of 8-hydroxy-delta cadinene in an amount of at least 10 mg per liter.