Patent ID: 8507249

Claim:
A method for increasing the production of a polypeptide secreted by a Type III secretion system (TTSS), the method comprising: a) providing an A/E pathogenic cell comprising a recombinant Orf11/Global Regulator of LEE Activator (GrlA) nucleic acid molecule derived from an A/E pathogen; b) expressing an Orf11/GrlA polypeptide encoded by the recombinant Orf11/GrlA nucleic acid molecule, and c) growing the A/E pathogenic cell under conditions that favour secretion of Type III antigens, whereby the production of the polypeptide secreted by a Type III secretion system (TTSS), is increased when compared to an A/E pathogenic cell that does not comprise the recombinant Orf11/GrlA nucleic acid molecule.