Patent ID: 7279309

Claim:
A process for producing a nematode-extracted anticoagulant protein (NAP) comprising: (a) culturing host cells encoding a NAP selected from the group consisting of rNAPc2 and rNAPc2/proline under conditions that result in expression of the NAP; (b) recovering NAP from said host cells in a recovery process that does not use centrifugation to remove cells and comprises separating NAP from said host cells and cellular debris to produce a recovered NAP preparation, wherein said recovery process comprises expanded-bed cation exchange chromatography; and (c) purifying a NAP from said recovered NAP preparation in a purification process that does not use reversed-phase high pressure liquid chromatography and that comprises performing hydrophobic interaction chromatography, wherein said recovering and purifying with hydrophobic interaction chromatography are conducted at a pH below pH 4.