Patent ID: 7892827

Claim:
A mutein derived from human neutrophil gelatinase-associated lipocalin (hNGAL), said mutein having binding specificity for the cytotoxic T lymphocyte-associated antigen (CTLA-4), wherein said mutein: (a) comprises the amino acid sequence of SEQ ID NO: 79 but for at least 12 amino acid replacements selected from the group consisting of A40R, A40Y, E44D, E44N, K46Q, K46R, D47H, D47Y, Q49M, K50Q, K50N, K50D, L70I, F71S, F71L, R72S, R72P, R72D, K73H, K73T, D77E, W79T, W79P, N96D, P101G, P101R, G102D, G102M, L103K, L103D, A125L, A125Q, V126A, S127E, S127N, S127G, Q128D, Q128H, N129D, R130A, R130T, Y132F, and Y132H; and (b) binds human CTLA-4 with a K D of 50 nM or less.