Patent ID: 7884200

Claim:
A retroviral vector for carrying a target gene specific insert into a cell in order to modify the expression of a target gene having a sense strand and an antisense strand, comprising: (a) an H1 promoter having the nucleotide sequence of: ccctttctcaccagagtatgtcttgaatattctaagggtttaggtttctgtaaagtgcaaataccactaa agggtcttgt gtatcgctgt acgtttataa (SEQ ID NO:14) (b) a polylinker region comprising a nucleotide sequence of gatccgctgg gactcctttg catgttcaag agacatgcaa aggagtccca gctttttgga aa (SEQ ID NO:4); and (c) a target gene specific insert comprising double stranded RNA, wherein said double stranded RNA comprises a sense portion that is complementary to a portion of the antisense strand of the target gene, and an antisense portion that is complementary to a portion of the sense strand of the target gene, so that the sense portion and antisense portion anneal, and the double stranded RNA folds back upon itself.