Patent ID: 7273699

Claim:
A method of detecting a nucleic acid sequence variation, wherein the sequence variation is a single-nucleotide polymorphism or a 1 nucleotide deletion, insertion or inversion, comprising: denaturing a first double-stranded nucleic acid and a second double-stranded nucleic acid, wherein the first double-stranded nucleic acid and the second double-stranded nucleic acid are each at least about 152 bp, and wherein the first double-stranded nucleic acid and the second double-stranded nucleic acid do not include a GC-clamp, thereby generating denatured nucleic acids in solution; allowing the denatured nucleic acids to reanneal, thereby generating reannealed nucleic acids in solution; increasing the temperature of the reannealed nucleic acids at a rate of about 0.067° C./minute or less; detecting a transition of the reannealed nucleic acids between a double-stranded state and a single-stranded state, thereby generating a melting profile for the reannealed nucleic acids; and comparing the melting profiles of the reannealed nucleic acids wherein a difference between the melting profiles of the reannealed nucleic acids indicates a sequence variation between the first double-stranded and second double-stranded nucleic acids.