Patent ID: 7563568

Claim:
A method for detecting activated CD3+ T-cells in a patient suffering from inflammation, comprising: obtaining a tissue or biological sample from a patient; labeling a polynucleotide, wherein the polynucleotide comprises a polynucleotide selected from the group consisting of: (a) a polynucleotide sequence as shown in SEQ ID NO:1 from nucleotide 123 to nucleotide 557; (b) a polynucleotide sequence as shown in SEQ ID NO:1 from nucleotide 57 to nucleotide 557; (c) a polynucleotide sequence as shown in SEQ ID NO:1 from nucleotide 21 to nucleotide 557; and (d) a polynucleotide sequence complementary to (a), (b) or (c), producing a first reaction product by incubating the tissue or biological sample with the labeled polynucleotide under conditions wherein the polynucleotide will hybridize to a complementary polynucleotide sequence in the tissue or biological sample under highly stringent conditions; visualizing the labeled polynucleotide in the tissue or biological sample; and comparing the level of labeled polynucleotide hybridization in the tissue or biological sample from the patient to a normal control tissue or biological sample, wherein an increase in the labeled polynucleotide hybridization to the patient tissue or biological sample relative to the normal control tissue or biological sample is indicative of activated CD3+ T-cells in the patient, wherein the sample contains CD3+ T-cells, and wherein said highly stringent wash conditions encompass washing in a solution of 0.1×-0.2×SSC with 0.1% sodium dodecyl sulfate (SDS) at 50-65° C.