Patent ID: 7858311

Claim:
A method for sequencing a single target nucleic acid molecule, said method comprising: i. immobilizing onto a support a polymerase nucleic acid complex comprising the single target nucleic acid molecule, a primer nucleic acid which complements a region of the single target nucleic acid molecule, at least one family A or family B nucleic acid polymerase, and an attachment complex, wherein said attachment complex: (a) is associated with the support; (b) attaches to said polymerase on both sides of a DNA binding cleft of the polymerase; and (c) entraps the single target nucleic acid molecule, to produce an immobilized polymerase nucleic acid complex; ii. contacting said immobilized polymerase nucleic acid complex with at least one type of labeled nucleotide triphosphate (NTP), wherein each NTP is labeled with a detectable label; iii. sequentially detecting the incorporation of a plurality of said at least one type of labeled NTP into a single molecule of said primer nucleic acid to generate a nucleotide sequence; and iv. determining the complement to the nucleotide sequence generated in step iii, wherein the complement is a nucleotide sequence of the single target nucleic acid molecule, thereby sequencing the single target nucleic acid molecule.