Patent ID: 7209836

Claim:
A method for automatically creating crosstalk-corrected data of a microarray wherein crosstalk is caused by overlapping dye emission spectra, the method comprising: providing a microarray substrate having three or more calibration dye spots, each of the calibration dye spots comprising a single pure dye; for each of the calibration dye spots, generating a dye image containing at least one of the calibration dye spots for each of a plurality of output channels; for each of the calibration dye spots, measuring an output of each of the output channels to obtain output measurements; computing a set of correction factors from the output measurements by computing crosstalk ratios based on spot brightness values for each of the calibration dye spots on each of the output channels wherein the spot brightness value includes the crosstalk contribution of all of the combinations of excitation wavelengths and dyes; applying the set of correction factors to quantitation data obtained from the generated microarray images containing spots having three or more dyes with excitation or emission spectra to obtain crosstalk-corrected data; and displaying or storing in memory said crosstalk-corrected data.