Patent ID: 7442289

Claim:
A thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising: an inert carrier material; at least one transducer layer exhibiting a specific electrical conductivity χ>10 4 Ω −1 cm −1 , during use of the biosensor; and at least one bioactive layer adapted to function as a diffusion barrier during use of the biosensor, the bioactive layer further defined as: comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor; exhibiting a specific electrical conductivity of χ<1Ω −1 cm −1 during use of the biosensor; and capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor.