Patent ID: 7993887

Claim:
A biologically pure culture of a microorganism of the genus Pseudomonas that produces an enzyme with phospholipase A 1 activity, wherein the microorganism has the following bacterial properties and the following physiological and biochemical properties; (A.) Bacterial properties (1) Cell shape: Width 0.8 to 1.0 micrometer, Length 2.0 to 3.0 micrometers (2) Gram stainability: Negative (3) Presence of spores: Negative (4) Mobility: Positive (5) Colony shape (Cultivation for 48 hours) (a) Diameter: 2.0 to 3.0 mm (b) Color tone: Lemon yellow (c) Shape: Circular (d) Topography: Lens shaped (e) Fringe: Whole border (f) Surface shape: Smooth (g) Transparency: Opaque (h) Viscosity: Butter-like (6) Growth temperature test (degree Celsius) (a) At 37 degrees Celsius: Positive (b) At 45 degrees Celsius: Weak reaction (7) Catalase reaction: Positive (8) Oxidase reaction: Positive (9) Production of acid and gas from glucose (Acid production/Gas production): Negative/Negative (10) O/F test (Oxidation/Fermentation): Negative/Negative (B.) Physiological and biochemical properties (1) Nitrate reduction: Negative (2) Indole production: Negative (3) Glucose acidification: Negative (4) Arginine dihydrolase: Positive (5) Urease: Negative (6) Esculin hydrolysis: Negative (7) Gelatin hydrolysis: Positive (8) Beta galactosidase: Negative (9) Glucose assimilation: Positive (10) L-Arabinose assimilation: Positive (11) D-Mannose assimilation: Positive (12) D-Mannitol assimilation: Positive (13) N-acetyl-D-glucosamine assimilation: Positive (14) Maltose assimilation: Negative (15) Potassium gluconate assimilation: Positive (16) n-Capric acid assimilation: Positive (17) Adipic acid assimilation: Negative (18) dI-Malic acid assimilation: Positive (19) Sodium citrate assimilation: Positive (20) Phenyl acetate assimilation: Negative (21) Cytochrome oxidase: Positive (22) Fluorochrome production on King's B agar medium: Positive.