Patent ID: 8252341

Claim:
A process for isolating growth and differentiating factors from colostrum, comprising: (a) subjecting a colostral solution to partial hydrolysis by adjusting the pH to about 3.75-3.85; (b) vortexing the partially hydrolyzed colostral solution obtained in step (a) for about 30 to 90 minutes; (c) adjusting the pH of the vortexed colostral solution obtained in step (b) to about 4.52-4.55; (d) centrifuging the pH-adjusted colostral solution obtained in step (c), and setting aside the resulting supernatant; (e) running the supernatant through a filtration system comprising one or more filtration columns; and (f) obtaining a fraction containing pools of growth and differentiating factors therefrom, all the while ensuring that the temperature during the process never exceeds about 38° C.