Patent ID: 8574827

Claim:
A method for identifying a compound that inhibits angiogenesis, the method comprising: assaying in vitro kinase activity of an Axl polypeptide comprising an amino acid sequence with greater than about 95% identity to full length SEQ ID NO: 4 in the presence of the compound, wherein the Axl polypeptide has kinase activity in the absence of said compound; performing a cell-based assay in an endothelial cell comprising said Axl polypeptide in the presence of the compound, which assay produces an angiogenesis phenotype selected from the group consisting of αvβ3 expression, tube formation, and haptotaxis in said endothelial cell in the absence of the compound; and identifying a compound that inhibits the in vitro kinase activity of the Axl polypeptide and that inhibits the angiogenesis phenotype in the cell-based assay, wherein inhibition of the in vitro kinase activity of the Axl polypeptide in the presence of the compound and inhibition of the angiogenesis phenotype in the cell-based assay in the presence of the compound identifies the compound as a compound that inhibits angiogenesis.