Patent ID: 8338153

Claim:
A recombinant polypeptide having hexose oxidase activity, wherein the polypeptide oxidizes at least maltose, and wherein the polypeptide comprises (a) a polypeptide encoded by the nucleic acid sequence set forth as SEQ ID NO: 30 or a nucleic acid sequence that hybridizes under stringent conditions to the nucleic acid sequence set forth as SEQ ID NO: 30, wherein said stringent conditions comprise (1) hybridization at 65° C. for at least 14 hours in a buffer containing 1×Denhardt's solution, 2×SSC, 0.1% dextran sulfate, and 50 gg/ml denatured salmon sperm DNA, followed by (2) two washes twice at 65° C. for 10 minutes in 2×SSC, 0.1% SDS, and followed by (3) two washes at 65° C. for 10 minutes in 1×SSC, 0.1% SDS; and (b) the polypeptide further comprises at least one of the amino acid sequences: (i) (SEQ ID NO: 1) Tyr-Glu-Pro-Tyr-Gly-Gly-Val-Pro, (ii) (SEQ ID NO: 2) Ala-Ile-Ile-Asn-Val-Thr-Gly-Leu-Val-Glu-Ser-Gly- Tyr-Asp-X-X-X-Gly-Tyr-X-Val-Ser-Ser-, (iii) (SEQ ID NO: 3) Asp-Leu-Pro-Met-Ser-Pro-Arg-Gly-Val-Ile-Ala-Ser- Asn-Leu-X-Phe- (iv) (SEQ ID NO: 4) Asp-Ser-Glu-Gly-Asn-Asp-Gly-Glu-Leu-Phe-X-Ala- His-Thr, (v) (SEQ ID NO: 5) Tyr-Tyr-Phe-Lys, (vi) (SEQ ID NO: 6) Asp-Pro-Gly-Tyr-Ile-Val-Ile-Asp-Val-Asn-Ala-Gly- Thr-X-Asp (vii) (SEQ ID NO: 7) Leu-Gln-Tyr-Gln-Thr-Tyr-Trp-Gln-Glu-Glu-Asp, and (viii) (SEQ ID NO: 8) X-Ile-Arg-Asp-Phe-Tyr-Glu-Glu-Met, where X represents an amino acid selected from the group consisting of Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr and Val.