Patent ID: 8911977

Claim:
A process for producing a peroxycarboxylic acid comprising: (a) providing a set of reaction components comprising: (1) at least one substrate selected from the group consisting of: (i) one or more esters having the structure [X] m R 5 wherein X=an ester group of the formula R 6 —C(O)O; R 6 =a C1 to C7 linear, branched or cyclic hydrocarbyl moiety, optionally substituted with hydroxyl groups or C1 to C4 alkoxy groups, wherein R 6 optionally comprises one or more ether linkages for R 6 =C2 to C7; R 5 =a C1 to C6 linear, branched, or cyclic hydrocarbyl moiety or a five-membered cyclic heteroaromatic moiety or six-membered cyclic aromatic or heteroaromatic moiety optionally substituted with hydroxyl groups; wherein each carbon atom in R 5 individually comprises no more than one hydroxyl group or no more than one ester group or carboxylic acid group; wherein R 5 optionally comprises one or more ether linkages; m=an integer ranging from 1 to the number of carbon atoms in R 5 ; and wherein said esters have solubility in water of at least 5 ppm at 25° C.; (ii) one or more glycerides having the structure wherein R 1 =a C1 to C21 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R 3 and R 4 are individually H or R 1 C(O); (iii) one or more esters of the formula: wherein R 1 =a C1 to C7 straight chain or branched chain alkyl optionally substituted with an hydroxyl or a C1 to C4 alkoxy group and R 2 =a C1 to C10 straight chain or branched chain alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkylheteroaryl, heteroaryl, (CH 2 CH 2 O) n , or (CH 2 CH(CH 3 )—O) n H and n is 1 to 10; (iv) one or more acylated monosaccharides, acylated disaccharides, or acylated polysaccharides; and (v) any combination of (i) through (iv); (2) a source of peroxygen; and (3) an enzyme catalyst comprising a polypeptide having perhydrolytic activity and an amino acid sequence having at least 90% identity to the amino acid sequence set forth in SEQ ID NO: 4, provided that the amino acid residue bound to the C-terminal side of the catalytic histidine is not glutamic acid; (b) combining the set of reaction components under suitable reaction conditions whereby peroxycarboxylic acid is produced; and (c) optionally diluting the peroxycarboxylic acid produced in step (b).