Patent ID: 7695973

Claim:
A method for ascertaining the extent to which a protein in a sample has been glycated, comprising: providing a biological sample containing both glycated and nonglycated forms of a protein; contacting the biological sample with a solid support at a first pH and binding both glycated and nonglycated forms of the protein to negatively charged groups immobilized on the solid support; obtaining a first measurement indicative of the total amount of the glycated and nonglycated forms of the protein bound to the solid support at the first pH; then changing the first pH on the solid support to a second pH, thereby removing both the glycated form of the protein and the nonglycated form of the protein from the negatively charged groups and inmiediately rebinding only the glycated form of the protein to hydroxyboryl groups immobilized on the solid support; obtaining a second measurement indicative of the amount of the glycated form of the protein bound to the solid support at the second pH; and relating the first measurement to the second measurement to ascertain the extent to which the protein in the sample has been glycated.