Patent ID: 8809034

Claim:
A method for removing mycoplasma contamination from cells contaminated with mycoplasma , the method comprising (i) treating cells contaminated with mycoplasma with an enzyme solution for single cell-isolation to obtain a cell suspension; (ii) removing cell clumps from the cell suspension obtained from the step (i); and (iii) culturing the cell suspension obtained from the step (ii) or cells harvested from the cell suspension in a medium containing an agent having a mycoplasma inhibitory activity wherein the agent having a mycoplasma inhibitory activity comprises (a) an extract of biomass obtained by culturing Amphidinium klebsii or Amphidinium carterae , wherein the extract is obtained by performing an extraction process which comprises: (a) extracting the biomass with a C 1 to C 4 alcohol and acetone; (b) extracting the extract obtained from the step (a) with water and ethyl acetate, followed by separating an aqueous layer; and (c) extracting the aqueous layer obtained from the step (b) with n-butanol, followed by separating an n-butanol layer; or (b) an amphidinol derivative selected from the group consisting of the compounds represented by Formula 1 to 8 below: thereby the cell culturing method removes mycoplasma contamination from cells contaminated with mycoplasma.