Patent ID: 8310678

Claim:
An analyzing method in which light from a sample set on a test piece is received by an image sensor having multiple pixels through an optical system having an optical filter and concentration information of the sample is obtained, the analyzing method comprising: a first step of calculating beforehand a luminance value obtained at any position in the image sensor based on dependence on a filter incident angle and wavelength characteristics of the light from the sample set with various known concentrations on the test piece; a second step of calculating correction coefficients for matching luminance distributions obtained at the respective concentrations in the first step with a luminance distribution of any reference concentration selected from the concentrations; a third step of making a rectilinear approximation by plotting the correction coefficients obtained at the respective concentrations in the second step, relative to the concentration information, a fourth step of calculating a correction function of the dependence on a filter incident angle according to the reference concentration, based on the luminance distribution of the reference concentration; a fifth step of correcting luminance obtained on the samples separately set to be measured with unknown concentrations on the test piece, by using the correction function obtained in the fourth step according to the reference concentration, and calculating the concentration information by using the corrected luminance; a sixth step of obtaining concentration correction coefficients of the samples to be measured, by correlating the concentration information obtained in the fifth step with a straight line determined in the third step; and a seventh step of integrating the concentration correction coefficients obtained in the sixth step to the correction function obtained in the fourth step according to the reference concentration, correcting the luminance of the samples to be measured, by using the correction functions after the integration, and obtaining again the concentration information of the samples to be measured, by using the corrected luminance.