Patent ID: 7101710

Claim:
A method for harvesting mesenchymal stem cells from human amniotic fluid comprising: (a) culturing a plurality of human amniotic fluid amniocytes including the steps of: (i) setting up primary amniocyte cultures comprising a plurality of adherent human amniotic fluid cells and a supernatant containing a plurality of non-adherent human amniotic fluid cells and a liquid medium; and (ii) collecting said non-adherent human amniotic fluid cells in the supernatant; and (b) culturing a plurality of mesenchymal stem cells including the steps of: (i) centrifuging the non-adherent human amniotic fluid cells; (ii) plating the centrifuged non-adherent human amniotic fluid cells from step b(i) in an alpha-modified Minimum Essential Medium supplemented with fetal bovine serum in a culture flask; and (iii) incubating said plated non-adherent human amniotic fluid cells from step (b)(ii) with humidified CO 2 to promote mesenchymal stem cell growth.