Patent ID: 7632981

Claim:
A process of producing a transgenic multi-cellular plant organism expressing a trait of interest and having a controlled distribution of said trait to progeny, wherein said process comprises sexual crossing of a first multi-cellular plant organism having a first heterologous DNA sequence comprising a first fragment of a nucleotide sequence conferring said trait of interest and a second multi-cellular plant organism having a second heterologous DNA sequence comprising a second fragment of the nucleotide sequence conferring said trait of interest, wherein at least one of said first and said second heterologous DNA sequences encodes a ribozyme for trans-splicing, and wherein the trans-splicing ribozyme is a Tetrahymena thermophila 26S rRNA, group I intron-derived ribozyme, whereby said first and said second heterologous sequences are designed such that said trait of interest arises due to RNA trans-splicing after said sexual crossing, and whereby said first and said second fragment of a nucleotide sequence conferring said trait of interest are present on allelic chromosomes, wherein said first and/or said second heterologous sequence contains an intron or a part thereof such that the RNA product of the trans-splicing reaction contains an intron capable of cis-splicing.