Patent ID: 7158903

Claim:
A method for deconvoluting contributions of a plurality of analytes in a sample comprising: obtaining a tandem mass spectrometry or MS n signal comprising a mass spectrum comprising at least one peak intensity for each of said target analytes in said sample where each of said peak intensities corresponds to a mass transition and where more than one of said analytes contributes to at least one of said peak intensities; providing a model comprising a system of linear equations relating said peak intensities to a contribution intensity for each of said analytes; calculating said contribution intensities of each of said analytes using said model; determining a branching ratio for each of said plurality of analytes at each of said mass transitions; calculating signal corresponding to said individual analytes; providing a calibration curve for at least one of said analytes relating contribution intensity to concentration; and determining, based on said calibration curve, a concentration of at least one analyte.