Patent ID: 8524485

Claim:
A recombinant oleaginous Yarrowia lipolytica host cell for improved production of at least one long-chain polyunsaturated fatty acid, said host cell comprising at least one isolated polynucleotide encoding a polypeptide having at least acyl-CoA:lysophospholipid acyltransferase activity, wherein the polypeptide comprises SEQ ID NO:19 and SEQ ID NO:20, wherein the polypeptide has at least 90% amino acid identity, based on the Clustal W method of alignment, when compared to SEQ ID NO:17; wherein the isolated polynucleotide is operably linked to at least one regulatory sequence, and wherein the total fatty acids of the host cell comprise at least 51% by weight eicosapentaenoic acid; and further wherein the host cell has an increase in C18 to C20 elongation conversion efficiency compared to a control host cell.