Patent ID: 8765371

Claim:
A kit for carrying out a method for preparing a multigene biomarker, wherein the multigene biomarker is characterized for the in vitro detection, differentiation or progress observation of a pathophysiological condition selected from the group consisting of: systemic inflammatory response syndrome (SIRS), sepsis (and their degrees of severity), sepsis-type conditions, septic shock, infectious/non-infectious multiple organ failure, survival probability in sepsis, focus of an infection, responders/non-responders to a particular therapy and cause of the pathophysiological condition, wherein the cause is associated with gram-positive or gram-negative bacteria, the method including the following steps: a) isolating sample nucleic acids from a sample originating from a patient; b) determining gene activities by means of polynucleotide sequences represented by SEQ ID NO: 1 to SEQ ID NO: 669 or their gene loci or transcripts thereof for forming at least one multigene biomarker that is characterized for the detection, differentiation or progress of the pathophysiological condition of the patient; c) determining gene activities of internal reference genes to which the gene activities determined under b) are related, in particular normalized; and d) forming an index value from the determined normalized gene activities of the multigene biomarker indicating the pathophysiological condition, wherein the interpretation of the index value results in the diagnosis of a pathophysiological condition in a patient, wherein the kit contains the polynucleotide sequences represented by SEQ ID NO: 1 to SEQ ID NO: 669 or their gene loci or transcripts thereof, and further wherein the multigene biomarker is specific for the patient's pathophysiological condition.