Patent ID: 8771973

Claim:
A method of identifying a non-anticoagulant sulfated polysaccharide capable of Factor VII-independent acceleration of blood clotting, the method comprising: measuring clotting time of a Factor VII-deficient plasma sample; contacting the Factor VII-deficient plasma sample with a non-anticoagulant sulfated polysaccharide, wherein the non-anticoagulant sulfated polysaccharide is pentosan polysulfate or fucoidin, at a concentration of about 10 nM to about 500 nM; measuring clotting time of the Factor VII-deficient plasma sample after contacting with the non-anticoagulant sulfated polysaccharide; and comparing the clotting time before contacting the Factor VII-deficient plasma sample with the non-anticoagulant sulfated polysaccharide with the clotting time after contacting the Factor VII-deficient plasma sample with the non-anticoagulant sulfated polysaccharide, wherein a decrease in clotting time after contacting the Factor VII-deficient plasma sample with the non-anticoagulant sulfated polysaccharide compared to before contacting the Factor VII-deficient plasma sample with the non-anticoagulant sulfated polysaccharide indicates that the non-anticoagulant sulfated polysaccharide is capable of Factor VII-independent acceleration of blood clotting.