Patent ID: 8367400

Claim:
A culture vessel for adherent cell growth in situ comprising: a body having at least a first edge at least partially perimetrically bounding said body; a first seal extending from said first edge, said first seal being breachable and fluid-tight, and disposed obliquely to said first edge so as to subtend an acute angle therewith, said first seal separating said body into first and second chambers; and, a second seal which separates said second chamber from a third chamber, said second seal being breachable and fluid-tight, said second chamber contiguously contacting said first and second seals, wherein, the collective volume of said first, second and third chambers is at least twice the collective volume of said first and second chambers; and wherein, said first chamber, said second chamber, said third chamber, said first seal and said second seal are configured to provide a constant rate of expansion of the collective volumes with the ratio of the collective volume of said first and second chambers to the volume of said first chamber being equal to the ratio of the collective volume of said first, second and third chambers to the collective volume of said first and second chambers, and to provide a constant rate of volume to surface ratio with the ratio of volume to surface area of said first chamber being equal to the ratio of the collective volume of said first and second chambers to the collective surface area of said first and second chambers, and equal to the ratio of the collective volume of said first, second and third chambers to the collective surface area of said first, second and third chambers.