Patent ID: 8128974

Claim:
A process of preparing a mustard seed protein isolate, which comprises: (a) extracting a mustard seed meal at a temperature of at least about 5° C. to cause solubilization of mustard seed protein in said mustard seed meal and to form an aqueous mustard seed protein solution having a mustard seed protein content of about 5 to about 30 g/L and a pH of about 5 to about 6.8, (b) separating the aqueous mustard seed protein solution from residual mustard seed meal, (c) increasing the protein concentration of said aqueous mustard seed protein solution to at least about 200 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a concentrated mustard seed protein solution, (d) diluting said concentrated mustard seed protein solution into chilled water having a temperature below about 15° C. to cause the formation of mustard seed protein micelles, (e) settling the mustard seed protein micelles to form an amorphous, sticky, gelatinous, gluten-like mustard seed protein micellar mass, and (f) recovering the mustard seed protein micellar mass from supernatant having a mustard seed protein content of at least about 100 wt % as determined by Kjeldahl nitrogen×6.25 on a dry weight basis.