Patent ID: 7759636

Claim:
A method for calibrating using a detector comprising a plurality of pixel elements in a biological analysis platform, the method comprising: obtaining measured emission data from a biological sample, wherein the measured emission data comprises a long measured sample signal and a short measured sample signal; determining an appropriately corresponding correction value for each of the plurality of pixel elements, which correction values have various dependencies on integration times and intensity, wherein the determination comprises: exposing the plurality of pixel elements to a calibration signal in a substantially uniform manner to generate a plurality of response signals wherein the intensity of the calibration signal and the acquisition time of the pixel elements are varied; evaluating the plurality of response signals to identify signal deviations in the pixel elements; and normalizing the response signals of the pixel elements to mitigate systematic signal deviations between the pixel elements for measured raw data; and evaluating the long measured sample signal for further processing with respect to a selected dynamic range, wherein when the long measured sample signal falls outside the selected dynamic range, the short measured sample signal value is selected for further processing.