Patent ID: 7662584

Claim:
A method of increasing the secretion of a heterologous protein in a fungal cell, comprising inducing an unfolded protein response (UPR) by increasing the presence of a HAC1 UPR-modulating protein in said fungal cell, comprising transforming the fungal cell with a nucleic acid encoding a yeast or filamentous fungal HAC1 UPR-modulating protein comprising a DNA binding domain consisting of: a) at least 90% sequence identity to the DNA binding domain of amino acid residues 84-147 of SEQ ID NO: 5; b) at least 90% sequence identity to the DNA binding domain of amino acid residues 53-116 of SEQ ID NO: 6 or c) the DNA binding domain of amino acid residues 45-109 of SEQ ID No:19, and increasing secretion of the heterologous protein relative to secretion of the heterologous protein in a parental cell, wherein said fungal cell is a yeast or filamentous fungal cell.