Patent ID: 8513397

Claim:
A method of producing a product of interest in a plant cell comprising: obtaining a first nucleic acid segment comprising at least one promoter, a short intergenic region (SIR) of a geminivirus genome, and a region encoding a first part of the product of interest, wherein the region encoding the first part of the product of interest is flanked on either side by at least a portion of a long intergenic region (LIR) of a geminivirus genome; obtaining a second nucleic acid segment comprising at least one promoter, a short intergenic region (SIR) of a geminivirus genome, and a region encoding a second part of a product of interest, wherein the region encoding the second part of the product of interest is flanked on either side by at least a portion of a long intergenic region (LIR) of a geminivirus genome; obtaining a third nucleic acid segment comprising a promoter and a nucleic acid encoding a Rep/RepA protein of a geminivirus genome; introducing the first nucleic acid segment, the second nucleic acid segment and the third nucleic acid segment into a plant cell to cause concomitant transient expression of the nucleic acid segments; and producing the product of interest in the plant cell by transient expression of the nucleic acid segment encoding the first and second parts of the product of interest; wherein the first nucleic acid segment and the second nucleic acid segment are separate replicons and are non-competing.