Patent ID: 8232086

Claim:
A method of producing a variant of a parent maltogenic alpha-amylase, said method comprising a) modeling the parent alpha-amylase on the three-dimensional structure of SEQ ID NO: depicted in the Appendix to produce a three-dimensional structure of the parent alpha-amylase; b) identifying in the three-dimensional structure obtained in step (a) at least one structural part of the parent wherein an alteration in said structural part is predicted to result in said altered property; c) modifying the sequence of a nucleic acid encoding the parent alpha-amylase to produce a nucleic acid encoding a deletion, insertion, or substitution of one or more amino acids at a position corresponding to said structural part; and d) expressing the modified nucleic acid in a host cell to produce the variant alpha-amylase, wherein the variant has alpha-amylase enzymatic activity and has at least one altered property relative to the parent.