Patent ID: 8003317

Claim:
A multiplex method of genotyping a plurality of polymorphic loci, comprising: (a) simultaneously amplifying a plurality of nucleic acid-target regions under amplification conditions whereby at least 60% of 7 or more nucleic acid target-regions attempted are amplified by 7 or more amplification primer pairs to produce an amplified mixture of nucleic acid-target regions containing polymorphic loci, wherein the amplification conditions comprise dNTPs; (b) after (a) contacting the amplified mixture of nucleic acid-target regions with 7 or more genotyping primers in the presence of at least one chain terminating reagent under primer mass extension conditions whereby the primers are extended up to, or through, the respective polymorphic loci, wherein there is one genotyping primer for each polymorphic locus and wherein the extension conditions comprise at least 55 cycles; (c) determining the mass of the extended genotyping primers; and (d) determining at least 60% of the genotypes for said 7 or more nucleic acid target-regions attempted.