Patent ID: 7771964

Claim:
A method for overproducing polyhydroxyalkanoate comprising: i) preparing transformed Escherichia coli comprising a phbCAB genes originated from Alcaligenes eutrophus , wherein the Escherichia coli is not ldh deficient; ii) seed-culturing the transformed Escherichia coli; iii) inoculating LB culture medium with cells resulting from the seed culture of step ii) to contain less than 2×10 4 cells per ml in the LB culture medium, and culturing the cells through growth phase; iv) producing the polyhydroxyalkanoate in the cultured cells during stationary phase; v) secreting the polyhydroxyalkanoate extracellularly from the cultured cells; and vi) purifying the polyhydroxyalkanoate, wherein the phbCAB genes comprises a phbC gene having the nucleotide sequence of SEQ ID NO: 1, a phbA gene having the nucleotide sequence of SEQ ID NO: 2 and a phbB gene having the nucleotide sequence of SEQ ID NO:3, fused from 5′ to 3′ in the order given, and glucose is added to the LB medium during the growth and/or the stationary phase such that the glucose concentration in the LB culture medium is less than 10%, w/v, immediately after adding the glucose.