Patent ID: 7435804

Claim:
A method for recovery of a ScFv antibody having the sequence shown in SEQ ID NO:2 or having the sequences shown in SEQ ID NOS: 3 and 4 from E. coli inclusion bodies in biologically active form which comprises: providing a transformed E. coli cell expressing a nucleic acid sequence encoding the ScFv antibody of SEQ ID NO:2 or SEQ ID NOS: 3 and 4 in the inclusion bodies; solubilizing the inclusion bodies in a detergent to release the ScFv antibody; oxidizing the released ScFv antibody to form disulfide bonds; removing the detergent; precipitating the oxidized ScFv antibodies; dissolving the precipitated ScFv antibodies in a denaturing solution; immobilizing the ScFv antibodies on a solid support; renaturing ScFv antibodies on the solid support; and eluting the ScFv antibodies in biologically active form, wherein the ScFv antibody binds interferon α-2b.