Patent ID: 8669093

Claim:
A genetically-engineered Escherichia coli comprising: a gene encoding an NADH-dependent D-lactate dehydrogenase (ldhA) obtained from Escherichia coli, a disruption in said Escherichia coli of an endogenous FAD-dependent D-lactate dehydrogenase (did) gene resulting in inactivation or reduction of its inherent activity, and a disruption in said Escherichia coli of an endogenous pyruvate formate-lyase (pfl) gene resulting in inactivation or reduction of its inherent activity, wherein said ldhA gene is integrated in the genome of said Escherichia coli and said ldhA gene product is expressed under the control of an Escherichia coli promoter that is not a promoter of the ldhA gene, and wherein said genetically-engineered Escherichia coli produces at least 113.5 g/L D-lactic acid at 30 hours when cultured.