Patent ID: 7115232

Claim:
A fluorescence validation microplate for testing the validity of a fluorescence microplate reader comprising a microplate having a plurality of wells therein, said plurality of wells comprising: (a) a first set of measurement wells in a 3×3 grid suitable for performing a cross-talk one-in-eight well design test wherein the center well of the first set contains a solid fluorophore and the outer wells of the first set contain no fluorophore; (b) a second set of measurement wells in a 3×3 grid suitable for performing a cross-talk eight-around-one well design test wherein the center of the second set contains no fluorophore and the outer wells of the second set each contains a solid fluorophore; (c) a third set of wells suitable for performing a linearity test wherein each well of the third set has a different concentration of solid fluorophore or the wells within the third set are decreasing in diameter; (d) a fourth set of wells suitable for performing an alignment test wherein each well of the fourth set has the same exact amount of solid fluorophore.