Patent ID: 8034575

Claim:
A process for determining whether a test compound modulates the binding of an RTP801 polypeptide to a second polypeptide comprising the following steps: (a) separately contacting the RTP801 polypeptide (SEQ ID NO:2 or a fragment thereof) with the second polypeptide, which is selected from the group consisting of a TSC1 polypeptide (SEQ ID NO:4 or a fragment thereof), a TSC2 polypeptide (SEQ ID NO:6 or a fragment thereof) and an alpha-tubulin polypeptide (SEQ ID NO:8 or a fragment thereof), both in the presence and in the absence of the test compound; and (b) determining the amount of complex formed between the RTP801 polypeptide or fragment thereof and the second polypeptide in the presence and in the absence of the test compound, wherein a difference in the amounts determined indicates that the test compound modulates the binding of the RTP801 polypeptide to the second polypeptide.