Patent ID: 8037739

Claim:
A method for analyzing a sample in a liquid, wherein a scanning probe microscope (SPM) is used for enabling a probe disposed on a front end of a cantilever to approach a surface of the sample immersed in the liquid, then the surface of the sample is scanned, and an interaction between the sample and the probe is detected, thereby generating an image, the method comprising: filling a sealing liquid, which is immiscible with a liquid used for an analysis, around the liquid used for the analysis, wherein the sample and the probe are immersed therein, so as to form a sealing state for isolating the liquid used for the analysis from an external gas, wherein the sealing state is formed through the following steps: adhering a liquid drop of the liquid used for the analysis to an inner part of a sample holder, which has an opened upper surface and is capable of being fully filled up with a liquid; adhering the liquid drop of the liquid used for the analysis to a lower surface of a base having the fixed cantilever in a manner of surrounding the cantilever; making the base to approach the sample holder, so as to blend the liquid drops of the liquid used for the analysis adhered respectively to the base and the sample holder; and feeding the sealing liquid into the sample holder for the sealing liquid to surround the liquid used for the analysis.