Patent ID: 6979442

Claim:
A stabilized protein composition consisting of a granulocyte colony stimulating factor (G-CSF) and a stabilizing buffer selected from the group consisting of N-2-hydroxyethylpiperazine-N′-2-aminoethane sulfonic acid (HEPES), N-tris-(hydroxymethyl)methyl-2-aminoethane sulfonic acid (TES), N-tris(hydroxymethyl) methylglycine (Tricene), 2-(N-morpholino)ethane sulfonic acid (MES), bis-(2-hydroxyethyl)imino-tris-(hydroxymethyl)methane (bis-tris), N-2-acetamidoiminodiacetic acid (ADA), N-(2-acetamido)iminodiacetic acid (ACES), piperazine-N,N′-bis (2-ethanesulfonic acid) (PIPS), 3-(N-morpholine)-2-hydroxypropane sulfonic acid (MOPSO), 1,3-bis[tris(hydroxymethyl)methylamino]propane(bis-tris propane), N,N-bis-(2-hydroxyethyl)-2-aminoethane sulfonic acid (BES), 3-(N-morpholine)propane sulfonic acid (MOPS), 3-[N-bis(hydroxyethyl)-amino]-2-hydroxypropane sulfonic acid (DIPSO), 3-[N-(tris-hydroxymethyl)methylamino]-2-hydroxypropane sulfonic acid (TAPSO), piperazine-N,N′bis-(2-hydroxypropane) sulfonic acid (POPSO), N-hydroxyethylpiperazine-N′-2-hydroxypropane sulfonic acid (HEPPSO), N-2-hydroxyethylpiperazine-N′-2-aminopropane sulfonic acid (EPPS), N,N-bis-(2-hydroxyethyl)glycine (bicine), N-tris(hydroxymethyl)methyl-3-aminopropane sulfonic acid (TAPS), 3-N-(α,α-dimethylhydroxuethyl)-amino-2-hydroxypropane sulfonic acid (AMPSO) and 3-N-cyclohexylamino sulfonic acid (CAPSO), wherein said composition maintains therapeutic levels of said protein for a sustained period, and said protein is present in an amount sufficient to provide therapeutic benefit to a mammal for a predetermined period of time.