Patent ID: 7700302

Claim:
A method for displaying a library of antibodies on the surface of yeast cells, comprising: providing yeast cells transformed with a library of yeast expression vectors encoding a library of antibodies each comprising an antibody heavy chain variable region (V H ) and an antibody light chain variable region (V L ), wherein at least one yeast expression vector comprises one or more homologous recombination sites and has undergone homologous recombination such that an insert polynucleotide sequence from a first polynucleotide sequence encoding the V H or a second polynucleotide sequence encoding the V L is incorporated into the yeast expression vector, and wherein the V H or V L polynucleotide sequence is fused in-frame with a sequence encoding a binding subunit of a yeast adhesion receptor; and expressing the library of antibodies encoded by the library of yeast expression vectors in the yeast cells, whereby the library of antibodies are displayed on the surface of the yeast cells.