Patent ID: 7749700

Claim:
A method of determining whether a human subject has had an ischemic stroke, comprising: detecting expression of ischemic stroke-related nucleic acid molecules of the subject obtained from a blood sample, wherein the ischemic stroke-related molecules comprise CD163 detectable by using any of SEQ ID NOS: 21-42; hypothetical protein FLJ22662 laminin A motif detectable by using any of SEQ ID NOS: 43-53; amyloid beta (A4) precursor-like protein 2 detectable by using any of SEQ ID NOS: 54-86; N-acetylneuraminate pyruvate lyase detectable by using any of SEQ ID NOS: 87-97; v-fos FBJ murine osteosarcoma viral oncogene homolog detectable by using any of SEQ ID NOS: 98-108; toll-like receptor 2 detectable by using any of SEQ ID NOS: 109-119; chondroitin sulfate proteoglycan 2 (versican) detectable by using any of SEQ ID NOS: 120-174; interleukin 13 receptor, alpha 1 detectable by using any of SEQ ID NOS: 175-218; CD14 antigen detectable by using any of SEQ ID NOS: 219-229; bone marrow stromal cell antigen 1/CD157 detectable by using any of SEQ ID NOS: 230-240; complement component 1, q subcomponent, receptor 1 detectable by using any of SEQ ID NOS: 241-262; paired immunoglobin-like type 2 receptor alpha detectable by using any of SEQ ID NOS: 263-284; Fc fragment of IgG, high affinity Ia, receptor for (CD64) detectable by using any of SEQ ID NOS: 285-295; ectonucleoside triphosphate diphosphohydrolase 1 detectable by using any of SEQ ID NOS: 296-328; CD36 antigen (collagen type I receptor, thrombospondin receptor) detectable by using any of SEQ ID NOS:329-350; adrenomedullin detectable by using any of SEQ ID NOS: 351-361; dual specificity phosphatase 1 detectable by using any of SEQ ID NOS: 362-383; cytochrome b-245, beta polypeptide (chronic granulomatous disease) detectable by using any of SEQ ID NOS: 384-394; leukotriene A4 hydrolase detectable by using any of SEQ ID NOS: 395-405; erythroblastosis virus E26 oncogene homolog 2 (avian) detectable by using any of SEQ ID NOS: 406-427; neuronal apoptosis inhibitory protein: Homo sapiens transcribed sequence with strong similarity to protein sp:Q13075 ( H. sapiens ) BIR1_HUMAN Baculoviral IAP repeat-containing protein 1 detectable by using any of SEQ ID NOS: 428-438; and KIAA0146 protein detectable by using any of SEQ ID NOS: 439-460; comparing expression of the ischemic stroke-related nucleic acid molecules to a control representing expression of the ischemic stroke-related nucleic acid molecules expected in a subject who has not had an ischemic stroke; and determining that the subject has had an ischemic stroke when the presence of an at least 4-fold increase in expression of the ischemic stroke-related nucleic acid molecules in the subject relative to the control is detected.