Patent ID: 7070930

Claim:
A method of making a DNA comprising a recombinant eukaryotic viral amplicon, the method comprising: (a) transducing a bacterial cell with a first DNA, wherein the first DNA comprises a DNA vector comprising (i) a portion of eukaryotic viral genome comprising an ITR, (ii) a regulatable anti-selection gene, (iii) a phage packaging site, and (iv) a deficient or conditionally-deficient lambdoid origin of replication, and a second DNA, which comprises a DNA segment with high homology to a DNA segment of the first DNA, and (b) transducing the bacterial cell with a defective phage or a non-defective phage, or activating a defective or non-defective lysogen within the cell, such that the deficient or conditionally-deficient lambdoid origin of replication is operable and the first DNA and the second DNA undergo a double homologous recombination event to form a DNA comprising a recombinant eukaryotic viral amplicon.