Patent ID: 8268575

Claim:
An aqueous solution for the in-vitro biosynthesis of reduced nicotinamide adenine dinucleotide (NADH) comprising: a nicotinamide phosphoribosyltransferase (Nampt) polypeptide at a concentration of about 0.01 μg/mL to about 100 μg/mL; a mammalian nicotinamide mononucleotide adenylyltransferase (Nmnat) polypeptide at a concentration of about 0.01 μg/mL to about 200 μg/mL; alcohol dehydrogenase protein (ADH); bovine serum albumen (BSA); ATP; phosphoribosyl pyrophosphate (PRPP); a magnesium ion; ethanol; and a buffer selected from the group consisting of HEPES and Tris, wherein the Nampt and the Nmnat are each at least partially purified from other cellular constituents.