Patent ID: 8222373

Claim:
An isolated humanized antibody that binds sphingosine-1-phosphate and comprises two heavy chains and two light chains, wherein: A. each heavy chain comprises a heavy chain variable domain comprising: (i) a sequence of amino acid residues having an amino acid sequence EVQLVQSGAEVKKPGESLKISCQSFGYIFIDHTIHWMRQMPGQGLE WMGAISPRHDITKYNEMFRGQVTISADKSSSTAYLQWSSLKASDT AMYFCARGGFYGSTIWFDFWGQGTMVTVSS (SEQ ID NO: 32, residues 20-140, inclusive); or (ii) a sequence of amino acid residues having at least about 80% sequence identity to the amino acid sequence of A(i), above, provided that in any event the heavy chain variable domain comprises a first complementarity determining region (CDR) comprising a sequence of amino acid residues DHTIH (SEQ ID NO: 13), a second CDR comprising a sequence of amino acid residues AISPRHDITKYNEMFRG (SEQ ID NO: 31), and a third CDR comprising a sequence of amino acid residues GGFYGSTIWFDF (SEQ ID NO: 15); and B. each light chain comprises a light chain variable domain comprising: (i) a sequence of amino acid residues having an amino acid sequence ETTVTQSPSFLSASVGDRVTITCITTTDIDDDMNWFQQEPG KAPKLLISEGNILRPGVPSRFSSSGYGTDFTLTISKLQPEDF ATYYCLQSDNLPFTFGQGTKLEIK (SEQ ID NO: 33, residues 21-127, inclusive); or (ii) a sequence of amino acid residues having at least about 80% sequence identity to the amino acid sequence of B(i), above, provided that in any event the light chain variable domain comprises a first CDR comprising a sequence of amino acid residues ITTTDIDDDMN (SEQ ID NO: 10), a second CDR comprising a sequence of amino acid residues EGNILRP (SEQ ID NO: 11), and a third CDR comprising a sequence of amino acid residues LQSDNLPFT (SEQ ID NO: 12).