Patent ID: 7247766

Claim:
A method of producing a double transgenic mouse whose genome incorporates DNA comprising a coding sequence encoding SEQ ID NO. 9 operably linked to a prion regulatory promoter sequence and a coding sequence which encodes SEQ ID NO. 1 operably linked to a Thy-1 regulatory promoter sequence, which method comprises: (a) introducing a DNA construct comprising a coding sequence which encodes SEQ ID NO. 9 and a prion regulatory sequence operably linked to the coding sequence into a mouse cell or embryo and generating a transgenic mouse from said cell or embryo; (b) introducing a DNA construct comprising a coding sequence which encodes SEQ ID NO. 1 and a Thy-1 regulatory sequence operably linked to the coding sequence into a mouse cell or embryo and generating a transgenic mouse from said cell or embryo; and (c) crossbreeding the transgenic mice generated in steps (a) and (b); wherein the double transgenic mouse exhibits increased pathology as demonstrated by increased deposition of A-beta peptide relative to an APP London single transgenic mouse.