Patent ID: 7297521

Claim:
A method for producing L-arginine, comprising culturing a coryneform bacterium in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium, wherein said coryneform bacterium has enhanced intracellular carbamoyl-phosphate synthetase activity as compared with a non-transformant strain and produces L-arginine, and wherein said activity is enhanced by increasing a copy number of a DNA encoding carbamoyl-phosphate synthetase, or by modifying an expression regulation sequence of the gene encoding carbamoyl-phosphate synthetase, and wherein said gene encoding carbamoyl-phosphate synthetase comprises a nucleotide sequence encoding a small subunit of carbamoyl-phosphate synthetase, and a nucleotide sequence encoding a large subunit of carbamoyl-phosphate synthetase, and wherein said nucleotide sequence encoding a small subunit of carbamoyl-phosphate synthetase is not less than 90% homologous to nucleotides 283 to 1461 of SEQ ID NO: 1 and hybridizes under stringent conditions to nucleotides 283 to 1461 of SEQ ID NO: 1, and said nucleotide sequence encoding a large subunit of carbamoyl-phosphate synthetase is not less than 90% homologous to nucleotides 1470 to 4808 of SEQ ID NO: 1 and hybridizes under stringent conditions to nucleotides 1470 to 4808 of SEQ ID NO: 1, and wherein said stringent conditions comprise washing at 60° C. in 1×SSC and 0.1% SDS.