Patent ID: 7332337

Claim:
A method of delivering a heterologous gene sequence linked to a promoter sequence into a T lymphocyte cell, a B-cell, a mast cell, or a combination thereof, each of said cells having a common binding receptor, comprising (a) contacting in vitro said cell with said chimeric adenoviral particle having increased tropism for said T lymphocyte cell, a B-cell, a mast cell, or a combination thereof, relative to an adenoviral particle comprising only native adenoviral sequences, said chimeric adenoviral particle comprising a viral protein coat and containing said heterologous gene sequence linked to a promoter sequence, wherein said viral protein coat consists of native viral coat proteins from an adenovirus of a serotype other than the serotype Ad51, and wherein said native viral coat proteins are penton base proteins and hexon proteins; and a modified fiber protein comprising a first adenoviral amino acid sequence from an adenoviral serotype Ad51 fiber protein comprising the contiguous sequence of said Ad51 fiber protein beginning at the carboxy terminus thereof and extending to but not including the N-terminus anchoring domain of said Ad51 fiber protein, and a second amino acid sequence from the fiber protein of the adenovirus of a serotype other than the serotype Ad51 comprising the N-terminus anchoring domain of the amino acid sequence of the fiber protein of the adenovirus of a serotype other than the serotype Ad35 or Ad51, wherein said first adenoviral amino acid sequence of said modified fiber protein is a ligand for said binding receptor and is not in contact with said penton base protein, and wherein said second amino acid sequence of said modified fiber protein is associated with said penton base proteins; and (b) allowing said chimeric adenoviral particle to transduce in vitro said T lymphocyte cell, a B-cell, a mast cell, or a combination thereof.