Patent ID: 7312074

Claim:
A synthetic construct which is a peptide display carrier package (PDCP), said construct comprising a complex of a recombinant single-stranded polynucleotide and a chimeric protein, wherein i) the chimeric protein has a) a nucleotide binding portion which comprises a binding domain of an estrogen receptor; and b) a target peptide portion displayed externally on the package, ii) said recombinant polynucleotide comprises a) a chimeric protein-encoding portion which encodes the chimeric protein of the complex; and b) a nucleotide sequence motif which is specifically bound by said nucleotide binding portion of the chimeric protein, and wherein the nucleotide binding portion of the chimeric protein is bound to the nucleotide sequence motif of the recombinant polynucleotide, and wherein the chimeric protein-encoding portion of the recombinant polynucleotide is not bound by the nucleotide binding portion of the chimeric protein, and wherein the chimeric protein-encoding portion of the recombinant polynucleotide is protected from degradation by a binding moiety which is a viral protein and which is bound non-specifically to the polynucleotide irrespective of nucleotide sequence, and wherein said construct is produced in a host cell transformed with said recombinant polynucleotide and extruded therefrom without lysis of the host cell.