Patent ID: 8524461

Claim:
An enzyme immunoassay construct for detecting a primate immunodeficiency virus, comprising: a first substrate to which is bound a plurality of detection multiple antigenic peptides with a same or different amino acid sequence, each detection multiple antigenic peptide comprising a portion of an immunodominant region of a transmembrane envelope protein of a primate immunodeficiency virus, wherein at least one simian immunodeficiency virus is represented in at least one of the detection multiple antigenic peptides; and a second substrate to which is bound a plurality of differentiation multiple antigenic peptides with a same or different amino acid sequence, each differentiation multiple antigenic peptide comprising a portion of a V3-loop of an envelope protein of a primate immunodeficiency virus, wherein at least one simian immunodeficiency virus is represented in at least one of the differentiation multiple antigenic peptides; wherein the detection multiple antigenic peptides consist of a core matrix and at least two linear antigenic sequences bonded to the core matrix by β-alanine and d-aspartic acid, each linear antigenic sequence is less than 16 amino acid residues and the β-alanine and d-aspartic acid serving as spacer amino acids between each linear antigenic sequence and the core matrix; and wherein the differentiation multiple antigenic peptides consist of a core matrix and at least two linear antigenic sequences bonded to the core matrix by diaminopropionic acid, each linear antigenic sequence is less than 16 amino acid residues and diminopropionic acid serving as a spacer amino acid between each linear antigenic sequence and the core matrix.