Patent ID: 7732161

Claim:
A fermentation method comprising: a. contacting a recombinant yeast with a fermentation medium wherein the recombinant yeast comprises a plurality of chromosomally integrated recombinant nucleic acid molecules, wherein each recombinant nucleic acid molecule encodes an enzyme that converts hydroxymethylglutaryl-CoA to L-mevalonic acid; wherein the recombinant yeast is capable of producing an isoprenoid product having 5, 10, 15 or 20 carbons and wherein the fermentation medium comprises a sugar carbon source in a first amount between about 1 gram per liter and about 100 gram per liter of fermentation medium; b. culturing the recombinant yeast under conditions wherein the sugar carbon source in the fermentation medium is consumed such that the fermentation medium comprises the sugar carbon source in a second amount which is less than the first amount; c. maintaining the culturing conditions such that the fermentation medium comprises the sugar carbon source in an amount that is equal to or less than the second amount; and d. recovering the product.