Patent ID: 7354711

Claim:
A method of identifying a compound that inhibits an immunostimulatory DNA-induced response, the method comprising contacting a cell with an immunostimulatory DNA, thereby inducing a measurable immune response, and a test compound and detecting inhibition of the immunostimulatory effect of the immunostimulatory DNA, wherein the test compound is selected from the group consisting of: compounds, other than chloroquine, having the structural formula: wherein R A is a hydrogen atom, a lower alkyl group, or linked to R B by a substituted or unsubstituted alkyl chain; R B is a hydrogen atom, an alicyclic group, an alkyl secondary, tertiary or quarternary amine, or an alkenyl secondary, tertiary or quarternary amine; R 2 is a hydrogen atom, a lower alkyl group, an aryl group, a heteroaromatic group, or a lower alkenyl group substituted with an aryl group; R 3 is a hydrogen atom, a lower alkyl group, or an aromatic group; R 5 is a hydrogen atom, a lower alkyl group, or a halogen atom; R 6 is a hydrogen atom, a lower alkyl group, a lower alkoxy group, an aryloxy group, an aryl group, an amino group or a thioether group; R 7 is a hydrogen atom, a lower alkyl group, a lower alkoxy group, an aryloxy group, a haloalkyl group, or a halogen atom; and R 8 is a hydrogen atom, or a lower alkoxy group, and pharmaceutically acceptable salts thereof, with the proviso that if R 7 is a halogen, then at least one of R 2 , R 3 , R 5 , R 6 or R 8 is non-hydrogen and R B is not 4-[N,N-dialkyl-n-pentylamine] or 4-[N-alkyl-N-hydroxyalkyl-n-pentylamine], compounds having the structural formula: wherein R B is a hydrogen atom, or an alkyl secondary or tertiary amino substitutent selected from the group consisting of 2-[N,N-dimethyl ethylamine], 2-[N-methyl ethylamine], 2-[ethyl-N,N-dialkyl amine], 2-[ethyl-N-alkyl amine], 2-[ethyl amine], 3-[propyl-N,N-dialkyl amine], 3-[propyl-N-alkyl amine], 4-[pentyl-N,N-dialkyl amine], 4-[pentyl-N-alkyl amine], 4-[pentyl amine], 4-[butyl-N,N-dialkyl amine], 4-[butyl-N-alkyl amine], 4-[butyl amine], 4-[N,N-diethyl pentylamine], 4-[N,N-diethyl butylamine], 4-[4-aryl-N,N-dialkyl butylamine], 4-[4-heteroaromatic-N,N-dialkyl butylamine], 4-[4-aryl-N-alkyl butylamine], 4-[4-heteroaromatic-N-alkyl butylamine], 4-[N,N-dialkyl pentylamine], and 7-[hepta-4-methyl-4-azaamine]; R 2 is a lower alkyl group; R 3 is a hydrogen atom or a lower alkoxy group; and X is a halogen atom, and pharmaceutically acceptable salts thereof.