Patent ID: 8680016

Claim:
A biochip based method for detecting nucleic acid binding proteins, comprising: 1) adding a solution containing several groups of nucleic acid capture probes to a biological sample containing target nucleic acid binding proteins, whereby complexes between the nucleic acid capture probes and the nucleic acid binding proteins are formed, wherein the nucleic acid capture probes contain at least a sequence that target nucleic acid binding proteins can bind, and wherein one strand of the nucleic acid capture probes in each group contains a 3′ overhang; 2) isolating the nucleic acid capture probe-nucleic acid binding protein complexes and collecting the nucleic acid capture probes in the complexes; 3) amplifying the collected nucleic acid capture probes by single-stranded PCR amplification using one primer, wherein the primer can hybridize to the 3′ overhang sequence of the nucleic acid capture probes; 4) hybridizing the amplified capture probes in step 3) with single stranded immobilization probes immobilized on the substrate of a biochip, wherein the immobilization probes contain a sequence complementary to the corresponding nucleic acid capture probes or one strand of the nucleic acid capture probes; and 5) detecting the hybridization result.