Patent ID: 8795968

Claim:
A method for preparing a molecule for sequencing, comprising the step of hybridizing to said molecule for sequencing a probe molecule of defined sequence and length, having a non-human sequence, a predefined 5′ end, a predefined 3′ end and a portion of a double stranded precursor molecule, said probe molecule further being prepared from said double stranded precursor molecule by a method comprising the steps of: (a) amplifying the double stranded precursor molecule with primers each containing a sequence homologous to a portion of the double stranded molecule and a sequence not homologous to the double stranded precursor molecule, said primers also containing a sequence comprising one of said predefined 5′ end or said predefined 3′ end, said primers further containing restriction enzyme cleavage sites to obtain an amplified double stranded molecule having cleavage sites at the 3′ and 5′ ends, said primers further comprising a first target sequence and a second, different, target sequence, wherein the first target sequence and the second target sequence hybridize to human genetic sequences; (b) cleaving the amplified double stranded molecule at the 5′ end with a first restriction enzyme; (c) cleaving the amplified double stranded probe molecule at the 3′ end with a second, different restriction enzyme to prepare the probe molecule of defined sequence and length; and (d) hybridizing said double stranded probe molecule of step (c) through said first target sequence and said second target sequence to said molecule for sequencing, thereby preparing said molecule for sequencing.