Patent ID: 8586330

Claim:
A method for producing a mature polypeptide, comprising: (a) cultivating a yeast or filamentous fungal host cell in a medium conducive for the production of said mature polypeptide, wherein the host cell comprises a signal peptide coding sequence operably linked to a mature polypeptide coding sequence, wherein the encoded signal peptide consists of amino acids 1 to 17 of SEQ ID NO: 2 which consists of a substitution at amino acid residue 2 with another single amino acid residue, and wherein the encoded signal peptide is directly fused in frame to the amino terminus of the encoded mature polypeptide; and wherein said host cell produces said mature polypeptide at a higher yield compared to an otherwise identical host cell that expresses said mature polypeptide directly fused to the signal sequence of SEQ ID NO: 2 without said amino acid substitution at residue 2; and (b) isolating the mature polypeptide from the cultivation medium.