Patent ID: 8101356

Claim:
A nucleic acid hybridization assay method comprising: contacting a nucleic acid hybridization assay device with a liquid sample including a target nucleic acid, wherein the nucleic acid hybridization assay device comprises a cleavable signal element comprising a capture probe of a single strand having a complementary sequence to the target nucleic acid; and a single stranded restriction probe not being complementary to the target nucleic acid, wherein one end of the single stranded restriction probe is ligated to the capture probe; and a solid support substrate attached to the other end of the single stranded restriction probe; hybridizing the cleavable signal element to the target nucleic acid present in the liquid sample; making the single stranded restriction probe into a double stranded restriction probe by DNA extension in the presence of a DNA polymerization solution using the target nucleic acid hybridized to the capture probe as a primer; cleaving the double stranded restriction probe by a restriction enzyme and/or the double stranded cleavable signal element by a cleavage enzyme, wherein the double stranded restriction probe is cleaved by the restriction enzyme specifically responsive to a double strand with a particular sequence and/or the cleavage enzyme is specifically responsive to double stranded nucleic acids; washing the nucleic acid hybridization assay device to remove the cleavable signal element cleaved by the restriction enzyme and/or the cleavage enzyme; and detecting whether the uncleaved signal element or the cleaved signal element exists on the solid support substrate.