Patent ID: 8592160

Claim:
A thermal cycler comprising: a holder to which a biotip is attached, the biotip having a longitudinal direction, and being charged with a reaction mixture and a liquid immiscible with the reaction mixture and having a smaller specific gravity than the reaction mixture; a first heating unit that heats a first end portion at an end of the longitudinal direction of the biotip attached to the holder; a rotating unit that rotates the holder; and a controller that has a first mode and a second mode, the first mode being a setting in which the rotation speed of the rotating unit is set to a first speed at which the magnitude of the centrifugal force that acts on the reaction mixture by the rotation of the rotating unit is smaller than the magnitude of the gravitational force that acts on the reaction mixture, the second mode being a setting in which the rotation speed of the rotating unit is set to a second speed at which the magnitude of the centrifugal force that acts on the reaction mixture by the rotation of the rotating unit is greater than the magnitude of the gravitational force that acts on the reaction mixture, the biotip being attached to the holder in such a direction that a distance between the first end portion of the biotip and the rotational axis of the rotating unit is shorter than a distance between the rotational axis and a second end portion representing an end of the longitudinal direction of the biotip and different from the first end portion, and that a gravitational potential of the first end portion is smaller than a gravitational potential of the second end portion.