Patent ID: 8802080

Claim:
A composition, comprising: (a) a first expression vector comprising a coding sequence of a first AAV capsid protein under control of an expression control sequence; and (b) a second expression vector comprising coding sequences for a second AAV capsid protein under control of an expression control sequence and a third AAV capsid protein under control of an expression control sequence; wherein the first and the second expression vectors are not on the same nucleic acid segment and wherein the first, the second, and the third capsid proteins are distinct capsid proteins selected from the group consisting of an AAV2 Vp1 protein, an AAV2 Vp2 protein and an AAV2 Vp3 protein, and further wherein the composition is modified 1) by a mutation in the coding sequence for the Vp2 protein such that the coding sequence for Vp2 does not express a functional AAV Vp2 capsid protein and/or 2) at least one mutation such that binding to HPSG is altered, impaired, or prevented wherein the at least one mutation is an arginine-to-alanine mutation or an arginine-to-lysine mutation at an amino acid residue R487, R585, or R588, wherein binding by the mutated capsid protein.