Patent ID: 8883972

Claim:
An isolated antibody that specifically binds to hydroxylated fibrinogen comprising at least one hydroxylated proline, wherein the isolated antibody has a higher affinity for the hydroxylated fibrinogen than for fibrinogen having no hydroxylated prolines, wherein when the hydroxylated fibrinogen is attached to a solid-phase in a reaction system for a competitive reaction between hydroxylated fibrinogen not attached to the solid-phase (antigen 1) and fibrinogen haying no hydroxylated prolines (antigen 2), the antibody satisfies measurement conditions in which 50% inhibition activity of an antibody bound by the antigen 1 is at least 10-fold when compared to 50% inhibition activity of an antibody bound by the antigen 2, and wherein the hydroxylated fibrinogen attached to the solid-phase and the antigen 1 are represented by the amino acid sequence TFP(OH)GFFSPMLGEFVSETESR (SEQ ID NO: 3) or ESSSHEIP(OH)GIAEFPSR (SEQ ID NO: 4) (P(OH) represents hydroxylated proline), and wherein the amino acid sequence of the antigen 2 is represented by TFPGFFSPMLGEFVSETESR (SEQ ID NO: 1) or ESSSHHPGIAEFPSR (SEQ ID NO: 2).