Patent ID: 7045305

Claim:
A method for localizing a probe within a cell, the method comprising: a) providing a sample comprising a cell expressing a membrane bound polypeptide, said polypeptide comprising a single chain antibody and an organelle targeting sequence wherein said single chain antibody binds to a specific ligand, wherein the ligand is phOx, wherein the membrane is a Golgi membrane, an Endoplasmic Reticulum (ER) or a plasma membrane; b) contacting the sample of a) with a membrane permeant probe/ligand conjugate, the probe/ligand conjugate comprising: i) a probe moiety, ii) a ligand comprising phOx, and iii) a linker moiety coupling the probe to the ligand; and c) detecting the probe/ligand conjugate within the cell, thereby localizing the probe within the cell.