Patent ID: 8914240

Claim:
A method for determining coronary artery disease risk in a subject comprising: (i) performing a reverse transcriptase polymerase chain reaction (RT-PCR) assay on a sample from the subject by using a plurality of distinct primer and probe sets that specifically hybridize to mRNA corresponding to each of the following genes consisting of: S100A12, CLEC4E, S100A8, CASP5, IL18RAP, TNFAIP6, AQP9, NCF4, CD3D, TMC8, CD79B, SPIB, HNRPF, TFCP2, RPL28, AF161365, AF289562, SLAMF7, KLRC4, IL8RB, TNFRSF 10C, KCNE3, and TLR4; (ii) generating, based on said assay, data representing mRNA expression levels corresponding to each of said genes; (iii) obtaining data representing age of the subject and gender of the subject; and (iv) generating, by a computer processor, a score indicative of coronary artery disease (CAD) risk by mathematically combining the data representing the mRNA expression levels, and the data representing the age and gender of the subject, wherein a higher score relative to a control subject having <50% stenosis in all major vessels indicates an increased likelihood that the subject has CAD.