Patent ID: 7915396

Claim:
An isolated nucleic acid encoding a humanized anti-TAG-72 CC49 monoclonal antibody, wherein the humanized anti-TAG-72 CC49 monoclonal antibody comprises a light chain comprising a light chain Complementarity Determining Region (L-CDR)1, a L-CDR2, a L-CDR3, and light chain framework regions 1-4 from HuCC49V10, a heavy chain comprising a heavy chain Complementarity Determining Region (H-CDR)1, a H-CDR2, a H-CDR3, and heavy chain framework regions 1-4 from HuCC49V10, wherein the residues at positions 5, 19 and 21 of SEQ ID NO: 1 (light chain framework region 1) are replaced with threonine, alanine, and isoleucine, respectively, and the residue at position 9 of SEQ ID NO: 4 (light chain framework region 4) is replaced with isoleucine, and wherein the residue at position 20 of SEQ ID NO: 5 (heavy chain framework region 1) is replaced with valine, the residues at positions 3 and 13 of SEQ ID NO: 6 (heavy chain framework region 2) are replaced with arginine and methionine, respectively, and the residues at positions 1, 2, 4, and 15 of SEQ ID NO: 7 (heavy chain framework region 3) are replaced with arginine, valine, isoleucine, and methionine, respectively; wherein the humanized CC49 antibody retains binding affinity for TAG-72 and has reduced immunogenicity, as compared to the HuCC49V10 antibody encoded by the nucleic acid sequences deposited as ATCC Accession No. PTA-5416.