Patent ID: 8715971

Claim:
A non-naturally occurring microbial organism having a 1,4-butanediol pathway and an isopropanol pathway, comprising at least one exogenous nucleic acid encoding a 1,4-butanediol pathway enzyme expressed in a sufficient amount to produce 1,4-butanediol and at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, wherein said 1,4-butanediol pathway comprises: (1) a succinyl-CoA reductase, a 4-hydroxybutyrate dehydrogenase, a 4-hydroxybutyryl-CoA transferase, a 4-hydroxybutyryl-CoA reductase (aldehyde-forming) and a 4-hydroxybutyraldehyde reductase; (2) a succinyl-CoA reductase, a 4-hydroxybutyrate dehydrogenase, a 4-hydroxybutyryl-CoA synthetase, a 4-hydroxybutyryl-CoA reductase (aldehyde-forming) and a 4-hydroxybutyraldehyde reductase; (3) a succinyl-CoA reductase, a 4-hydroxybutyrate dehydrogenase, a 4-hydroxybutyrate reductase and a 4-hydroxybutyraldehyde reductase; (4) a succinyl-CoA reductase, a 4-hydroxybutyrate dehydrogenase, a 4-hydroxybutyrate kinase, a phosphotrans-4-hydroxybutyrylase, a 4-hydroxybutyryl-CoA reductase (aldehyde-forming) and a 4-hydroxybutyraldehyde reductase; or (5) a succinyl-CoA reductase, a 4-hydroxybutyrate dehydrogenase, a 4-hydroxybutyrate kinase, a 4-hydroxybutyryl-phosphate reductase and a 4-hydroxybutyraldehyde reductase, and wherein said isopropanol pathway comprises: (6) an acetyl-CoA acetyl thiolase, an acetoacetyl-CoA transferase, an acetoacetate decarboxylase and an isopropanol dehydrogenase; (7) an acetyl-CoA acetyl thiolase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase and an isopropanol dehydrogenase; or (8) an acetyl-CoA acetyl thiolase, an acetoacetyl-CoA synthetase, an acetoacetate decarboxylase and an isopropanol dehydrogenase.