Patent ID: 7723040

Claim:
A method of detecting HIV-1 nucleic acid in a biological sample, comprising the steps of: contacting a biological sample containing HIV-1 nucleic acid with at least one capture oligomer comprising an LTR-specific sequence consisting of SEQ ID NO:1 or SEQ ID NO:19 or the LTR-specific sequence contained in SEQ ID NO:2 or SEQ ID NO:20 that hybridizes specifically to a target region in LTR sequences of HIV-1 nucleic acid and at least one capture oligomer comprising a pol-specific sequence consisting of SEQ ID NO:3 or SEQ ID NO:5 or the pol-specific sequence contained in SEQ ID NO:4 or SEQ ID NO:6 that hybridizes specifically to a target region in pol sequences of HIV-1 nucleic acid, thus forming a capture oligomer:HIV-1 nucleic acid complex; separating the capture oligomer:HIV-1 nucleic acid complex from the biological sample; amplifying LTR sequences, or a cDNA made therefrom, by using at least two amplification oligomers, wherein a first amplification oligomer is selected from the group consisting of SEQ ID NO:7 optionally with a promoter sequence joined to its 5′ end, SEQ ID NO:8, SEQ ID NO:29 optionally with a promoter sequence joined to its 5′ end, SEQ ID NO:30, SEQ ID NO:31 optionally with a promoter sequence joined to its 5′ end, SEQ ID NO:32, SEQ ID NO:33 optionally with a promoter sequence joined to its 5′ end, and SEQ ID NO:34, and wherein a second amplification oligomer is selected from the group consisting of SEQ ID NO:9, SEQ ID NO:35, and SEQ ID NO:36, and a nucleic acid polymerase in vitro to produce an amplified product of LTR sequences; and detecting the amplified product of LTR sequences using a labeled detection probe that hybridizes specifically with LTR sequence in the amplified product, thereby indicating presence of the HIV-1 nucleic acid in the biological sample.