Patent ID: 8889389

Claim:
An isolated DNA sequence comprising a promoter which has a promoter activity when said isolated DNA sequence is transformed into a Brevibacillus genus bacterium, and a DNA sequence encoding a protein A-like protein, wherein said protein A-like protein lacks the cell wall-binding domain X from protein A, and wherein the protein A-like protein is selected from the group consisting of proteins (a) to (c): (a) a protein with immunoglobulin-binding activity, which comprises an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence of either SEQ ID NO: 2 or SEQ ID NO: 4 without cell wall-binding domain X, (b) a protein with immunoglobulin-binding activity, which comprises an arbitrary portion of the amino acid sequence that constitutes the protein (a), and (c) a partial sequence of protein A.