Patent ID: 6875568

Claim:
A method for hybridizing one or more nucleic acid molecules, said method comprising i) contacting one or more double-stranded nucleic acid molecules with a denaturant selected from the group consisting of a) one or more amino acid denaturants, b) imidazole, and c) one or more amino acid denaturants plus imidazole, thereby forming one or more single-stranded target nucleic acid molecules; and ii) combining said one or more single-stranded target nucleic acid molecules with one or more additional nucleic acid molecules wherein said one or more additional nucleic acid molecules are capable of hybridizing to said single-stranded target nucleic acid molecules thereby obtaining one or more of said hybridized nucleic acid molecules; wherein said amino acid denaturants are selected from the ground consisting of one or more amino acids, polyamino acids, and combinations thereof; wherein said amino acid denaturants denature or separate double-stranded nucleic acid molecules.