Patent ID: 8586322

Claim:
A method for determining an immunosuppressant drug in a sample suspected of containing the immunosuppressant drug, the method comprising: (a) providing in combination in a medium: (i) the sample, (ii) a first monoclonal antibody for the immunosuppressant drug, and (iii) a second monoclonal antibody for the immunosuppressant drug, wherein the second monoclonal antibody binds to a portion of the immunosuppressant drug other than the portion to which the first monoclonal antibody binds to the immunosuppressant drug, (b) incubating the medium under conditions for binding of the first monoclonal antibody and the second monoclonal antibody to the immunosuppressant drug, and (c) examining the medium for the presence of an immunocomplex comprising the immunosuppressant drug, the first monoclonal antibody and the second monoclonal antibody, the presence and/or amount of the immunocomplex indicating the presence and/or amount of the immunosuppressant drug in the sample, wherein the immunosuppressant drug is selected from the group consisting of tacrolimus, cyclosporin, rapamycin and everolimus.