Patent ID: 8790897

Claim:
A single-chain fusion protein comprising: (a) a light chain (L-chain) or L-chain fragment of a clostridial neurotoxin, which L-chain or L-chain fragment includes the active proteolytic enzyme domain of the L-chain; (b) a targeting domain comprising or consisting of a growth factor; (c) a translocating domain that translocates the L-chain or L-chain fragment into the target cell; and (d) a site for cleavage by a proteolytic enzyme; and wherein said polypeptide lacks a functional heavy chain (H-chain) of a clostridial neurotoxin and therefore does not bind to clostridial neurotoxin receptors with the same affinity as native clostridial neurotoxin; wherein the growth factor is selected from the group consisting of platelet-derived growth factor, keratinocyte growth factor, and hepatocyte growth factor; and wherein the translocating domain is a clostridial neurotoxin heavy chain H N domain or a translocating domain from a bacterial toxin or a viral toxin.