Patent ID: 8715970

Claim:
A biocatalytic method of preparing a mono-acylated polyol of the general formula (I): wherein R 1 represents an optionally substituted, linear or branched, saturated or non-saturated hydrocarbyl residue; and A represents an optionally substituted, linear or branched hydrocarbylene residue having at least two carbon atoms, which method comprises a) reacting a polyol of the formula (II) and an acyl donor compound of the formula (III) wherein R 1 and A are as defined above, and Don represents a donor molecule residue carrying the said acyl group; in the presence of a mutated triacylglycerole lipase (EC 3.1.1.3) until a mono-acylated polyol of the above formula (I) is formed; and b) obtaining a monoacylated polyol product wherein said lipase is a mutant of Candida antarctica lipase B (CALB) comprising an amino acid sequence of SEQ ID NO: 2, mutated in at least one position and up to fifteen positions; wherein the amino acid comprises a mutation in position Thr40.