Patent ID: 7919046

Claim:
A method for covalent grafting at least one molecule of interest to the surface of a silicon substrate comprising surface hydride functions, said substrate carrying at least one spacer, which comprises: (a) anchoring covalently, to the surface of said substrate, at least one spacer compound of general formula (I) below: (X) n -E-(Y) m (I) in which: E represents a group chosen from alkyl and aryl radicals, X represents a C═C double bond, Y represents an end functional group chosen from triple bonds between two carbon atoms, triple bonds between a carbon atom and a nitrogen atom and azide groups, said functional group being capable of reacting with at least one complementary functional group Z carried by a molecule of interest, in a reaction resulting in the formation of a covalent bond between a carbon atom and a heteroatom of the functional groups Y and Z; Y and Z being chosen among the following pairings of functions: triple bonds between two carbon atoms (alkyne)/azide functions, and triple bonds between a carbon atom and a nitrogen atom (nitrile)/azide functions, with the proviso that when the functional group Y is the first member of the above pairings, the functional group Z is the second member and the converse, m and n, independently of one another, are integers between 1 and 3 inclusive, and (b) coupling covalently, to said spacer compound of formula (I) thus anchored to the surface of said substrate, at least one molecule of interest by formation, according to a click chemistry reaction, of at least one covalent bond of —C-Het-type in which Het is a heteroatom, by reaction of at least one functional group Z carried by said molecule of interest and chosen from triple bonds between two carbon atoms, triple bonds between a carbon atom and a nitrogen atom and azide functions, and of at least one group Y of said spacer compound of formula (I), Z and Y being capable of forming, together, said covalent bond; Y and Z being chosen among the following pairings of functions: triple bonds between two carbon atoms (alkyne)/azide functions, and triple bonds between a carbon atom and a nitrogen atom (nitrile)/azide functions, with the proviso that when the functional group Y is the first member of the above pairings, the functional group Z is the second member and the converse.