Patent ID: 8206949

Claim:
A method of producing a protein having reduced O-linked glycosylation comprising: (a) providing a nucleic acid encoding a protein operably linked to an inducible promoter; (b) introducing the nucleic acid into a yeast host cell or filamentous fungal host cell to provide a culture of the host cell; (c) growing the culture for a time sufficient to provide a multiplicity of the host cells having the nucleic acid before contacting the culture with one or more inhibitors of Pmt-mediated O-linked glycosylation; (d) contacting the culture with an inducer of the promoter to induce expression of the protein at a time before or at the same time as contacting the culture with the one or more inhibitors of Pmt-mediated O-linked glycosylation; and (e) isolating the glycoprotein produced by the host cell in the presence of the one or more inhibitors to produce the protein having reduced O-linked glycosylation.