Patent ID: 7838233

Claim:
A method for preparation of a sample to form a sample preparation including a lysate, said sample comprising at least one species of nucleic acid and a species of protein, whereby the method comprises: A) providing a sample which comprises at least one species of a nucleic acid and a protein, B) lysis of the sample in the presence of a fluid preparation to form a lysate, whereby the fluid preparation comprises a pH of about 7.1 to about 14, and whereby the fluid preparation comprises at least one nitrogenous compound which is at least one selected from the group consisting of: a) polyamines; b) amino acids, and oligo and polypeptides; c) nitrogenous heterocyclic compounds including homo or hetero polymers which comprise at least one nitrogenous compound; d) amines of the type R 1 R 2 NR 3 , whereby R 1 , R 2 und R 3 are chosen independently from one another and are selected from the group consisting of H, C 1 -C 5 -alkyl groups and aryl groups, whereby R 1 , R 2 and R 3 are not H simultaneously; e) carboxylic acid amides; f) inorganic ammonium salts; g) inner salt compounds containing ammonium groups; h) antibiotics binding nucleic acid; and i) compounds which bind in the small cavity of DNA; wherein the amount of nitrogenous compound is chosen so as to minimize precipitation of nucleic acid and/or protein during lysis or in the lysate produced; and further wherein the ratio of concentration of the at least one species of nucleic acid and protein in the lysate is the same as the ratio of concentration of the at least one species of nucleic acid and protein in the sample prior to said lysis.