Patent ID: 8691529

Claim:
A method for increasing the production of an antibody or fragment thereof, said method comprising: a) introducing into a eukaryotic cell a polynucleotide encoding an ABC50 protein or active fragment thereof having protein synthesis increasing activity and/or eIF2 binding activity and optionally encoding a selectable marker into a eukaryotic cell, wherein said eukaryotic cell expresses an antibody or a fragment thereof or wherein after the introduction of the polynucleotide encoding an ABC50 protein or active fragment thereof the eukaryotic cell is fused to a second eukaryotic cell expressing an antibody or fragment thereof, thus producing a eukaryotic cell expressing ABC50 protein or active fragment thereof and an antibody or a fragment thereof, and b) incubating the eukaryotic cell expressing the ABC50 protein or active fragment thereof and the antibody or a fragment thereof under suitable conditions for a suitable length of time to produce the antibody or fragment thereof in the presence of the ABC50 protein such that the antibody or fragment thereof is produced at higher levels than in the absence of the ABC50 protein.