Patent ID: 7691597

Claim:
A method for selecting a microbial transglutaminase formulation for binding application comprising: (a) measuring a protease activity of a plurality of microbial transglutaminase-containing products by a measurement method; and (b) selecting a microbial transglutaminase-containing product with a ratio of protease activity to transglutaminase activity (units of protease activity/units of transglutaminase activity) no more than 0.00024, wherein said measurement method comprises: (i) preparing an aqueous solution of a microbial transglutaminase-containing product and an aqueous solution of a protease substrate in the form of dimethylcasein so that the ratio of transglutaminase activity to the quantity of dimethylcasein is 200 units/g or less; (ii) conducting an enzymatic reaction based on protease; (iii) adding an acid and filtering; and (iv) measuring the concentration of said protease substrate in the filtrate.