Patent ID: 8772202

Claim:
A method of preparing a substrate for selective immobilization of an enzyme thereon, comprising: providing a substrate having a first surface component and a second surface component where the first and second surface components have different surface characteristics, wherein the substrate comprises a layer of the second surface component on a layer of the first surface component, and wherein the substrate comprises a zero mode waveguide disposed through the second surface component layer to the first surface component layer and exposing a portion of the first surface component layer, the portion of the first surface component layer thereby exposed forming a bottom reading surface of the zero mode waveguide; and contacting the substrate with a composition that selectively associates with the second surface component, which composition comprises a compound comprising one or more phosphonic acid groups, thereby passivating the second surface component and preparing the substrate for selective immobilization of the enzyme atop the bottom reading surface in the zero mode waveguide.