Patent ID: 7534424

Claim:
A method for increasing the level of a therapeutic gene product in a subject, the method comprising administering to said subject (a) a first viral vector comprising a therapeutic nucleic acid encoding said therapeutic gene product, wherein said therapeutic gene product is expressed through operable linkage of said nucleic acid to a promoter, and (b) an agent that reduces Kupffer cell function, wherein said agent is a second viral vector that does not comprise said therapeutic nucleic acid; wherein said second viral vector is the same type as said first viral vector; wherein said agent is administered less than 24 hours prior to or concurrently with administration of said first viral vector; wherein said first viral vector and said agent are not conjugated; wherein said agent is administered by a route selected from the group consisting of direct administration to the liver, intravenous administration, or intraperitoneal administration; wherein said first viral vector and said agent reach the liver following administration; and wherein levels of said therapeutic gene product are increased in hepatocytes by administration of said agent.