Patent ID: 7833708

Claim:
A microfluidic device, comprising (a) a substrate; (b) a first plurality of flow channels formed within the substrate, each of the first plurality of flow channels parallel to each other; (c) a second plurality of flow channels formed within the substrate, each of the second plurality of flow channels parallel to each other and in fluid communication with an inlet, the second plurality of flow channels orthogonally intersecting the first plurality of flow channels to define an array of reaction chambers; (d) isolation valves selectively actuatable to regulate solution flow to the reaction chambers, and to block flow between reaction chambers along at least one of: a flow channel in the first plurality of flow channels; or a flow channel in the second plurality of flow channels; (e) a plurality of temperature regions located along each of the second plurality of flow channels; and (f) a temperature controller operatively disposed to regulate temperature at one or more of the temperature regions.