Patent ID: 7608433

Claim:
A method for detecting the presence of classical swine fever virus (CSFV) ribonucleic acids in a biological sample, said method comprising: (i) obtaining a biological sample, said biological sample comprising ribonucleic acids; (ii) reverse transcribing said ribonucleic acids to obtain cDNA; (iii) contacting said cDNA with a first forward primer, a first reverse primer, and a DNA polymerase to produce a first PCR product, wherein the first forward primer consists of SEQ ID NO: 2, and the first reverse primer consists of SEQ ID NO: 4; (iv) hybridizing to the first PCR product a first nucleic acid probe consisting of SEQ ID NO: 3; (v) contacting an internal control ribonucleic acid derived from SEQ ID NO: 10 with a second forward primer and a second reverse primer to produce a second PCR product, wherein the second forward primer hybridizes to a target site corresponding to nucleotides of SEQ ID NO: 10 or a complementary strand thereof, and the second reverse primer hybridizes to a target site corresponding to nucleotides of SEQ ID NO: 10 or a complementary strand thereof; (vi) hybridizing to the second PCR product a second nucleic acid probe that hybridizes to a target site corresponding to nucleotides of SEQ ID NO: 10 or a complementary strand thereof; and (vii) detecting hybridization of said first nucleic acid probe to said first PCR product, wherein hybridization of said first nucleic acid probe to said first PCR product is indicative of the presence of CSFV nucleic acids in the biological sample.