Patent ID: 8802365

Claim:
A method of identifying a candidate modulator of NOTUM activity comprising: (a) providing a composition comprising: (1) an isolated NOTUM polypeptide; (2) a reporter molecule comprising a GPI anchor and a detectable moiety; and (3) a test compound, wherein the reporter molecule is attached to a membrane via the GPI anchor; (b) maintaining the composition for a suitable time period; (c) and measuring the amount of detectable moiety released from the membrane during the time period, wherein release of a greater amount of detectable moiety than would occur in the absence of the test compound indicates that the test compound is a candidate enhancer of NOTUM activity, and release of a lower amount of the detectable moiety as compared with the amount that would occur in the absence of the test compound indicates that the test compound is a candidate inhibitor of NOTUM activity.