Patent ID: 8716554

Claim:
A transgenic plant comprising at least one cell transformed with a DNA construct for generating siRNAs comprising the Col-5 polynucleotide of Meloidogyne javanica gene sequence as set forth in SEQ ID NO:4, wherein the plant is resistant to the development of subsequent generations of the parasite in or on said plant, wherein the DNA construct comprises an expression cassette comprising: a) at least one plant expressible promoter operably linked to; b) a polynucleotide sequence encoding a double stranded RNA, comprising: i) a first nucleotide sequence of at least 20 contiguous nucleotides having at least 90% sequence identity to the sense nucleotide sequence of the sequence set forth in SEQ ID NO:4; ii) a second nucleotide sequence of at least 20 contiguous nucleotides having at least 90% sequence identity to the complementary sequence of the sense nucleotide sequence of the sequence set forth in SEQ ID NO:4; and optionally; iii) a transcription termination signal.