Patent ID: 8883173

Claim:
A method of in vitro detection of specific anti- Mycobacterium paratuberculosis antibody comprising in a first step contacting a biological sample with an antigen for an amount of time sufficient to form an antigen-antibody complex, wherein the antigen is selected from the group consisting of: (a) a synthetic peptide 5P having the following formula: DPhe-NMeVal-Ile-Phe-Ala-OMe (SEQ ID NO: 1); (b) a lipopeptide L5P consisting of the synthetic peptide a) wherein the N-terminal phenylalanine residue is N-acylated with an eicosanoic acid acyl chain; and (c) a variant of peptide (a) or lipopeptide (b) which reacts with anti- Mycobacterium paratuberculosis antibodies, and in a second step detecting the formation of the antigen-antibody complex in said biological sample, wherein the presence of the antigen-antibody complex indicates the presence of the anti- Mycobacterium paratuberculosis antibody.