Patent ID: 8790928

Claim:
A method of amplifying gene expression in a moss plant cell by increasing the copy number of integrated transforming DNA constructs, the method comprising: 1) providing at least a first heterologous nucleic acid construct comprising at least one heterologous nucleotide sequence operably linked to a promoter, wherein said construct is flanked at the 5′ end thereof by a first recombination sequence and is flanked at the 3′ end of said construct by a second recombination sequence; 2) providing at least a second heterologous nucleic acid construct different from the first, said second construct comprising at least one heterologous nucleotide sequence operably linked to a promoter, wherein said construct is flanked at the 5′ end thereof by said second recombination sequence and is flanked at the 3′ end of said construct by said first recombination sequence; 3) transforming into the moss plant cell at least said first and said second heterologous nucleic acid constructs; and 4) regenerating the transformed moss plant cell into moss protonema comprising a plurality of copies of said at least one heterologous nucleotide sequence; wherein said first recombination sequence differs from said second recombination sequence and wherein recombination occurs between said different constructs at said first recombination sequences and at said second recombination sequences after transformation.