Patent ID: 8349619

Claim:
A method of detecting and analyzing protein interactions, which comprises the steps: a) expressing in an isolated host cell: a1) a first fusion protein comprising a first interaction partner and a functional protease and a2) a second fusion protein comprising a second interaction partner, a functional transcription factor domain, and a further domain causing anchoring outside the nucleus, with at least the functional transcription factor domain being proteolytically removable, from the domain which causes the second fusion protein to be anchored outside the nucleus, via a recognition and cleavage site of the functional protease, and b) interaction of the first and second interaction partners resulting in cleavage of the functional transcription factor domain from the second fusion protein, c) transport of the functional transcription factor domain into the nucleus resulting in activation of a reporter system, and d) detecting a signal generated by the reporter system wherein, detecting a signal generated by the reporter system in the presence of the first fusion protein, but not in the absence of the first fusion protein, is a means for detecting and analyzing protein interactions.