Patent ID: 6875748

Claim:
A method for expressing a polypeptide in a vertebrate, comprising administering into a tissue or cavity of said vertebrate a composition comprising: (a) about 1 ng to about 30 mg of a polynucleotide in aqueous solution, wherein the polynucleotide expresses a polypeptide upon delivery to vertebrate cells in vivo; (b) a salt M-X dissolved in said aqueous solution at a molar concentration ranging from about 50 mM to about 250 mM, and reaction, association, and dissociation products thereof, wherein M is a cation selected from the group consisting of sodium and potassium, wherein X is an anion selected from the group consisting of phosphate, acetate, bicarbonate, sulfate, and pyruvate; and (c) an auxiliary agent selected from the group consisting of a poloxamer and a reverse poloxamer; wherein said aqueous solution contains chloride ion at a molar concentration ranging from 0 mM to about 50 mM, and wherein said polypeptide is expressed in the vertebrate in an amount sufficient to be detectable.