Patent ID: 8323488

Claim:
A method of performing nano-liquid chromatography comprising: providing a nano-liquid chromatography apparatus on a chip, the apparatus comprising: a column defined between a substrate and a deposited Parylene layer, the substrate comprising a first moat extending along a first lateral side of the column and a second moat extending along a second lateral side of the column, wherein a first side edge of the Parylene layer is disposed in the first moat and a second side edge of the Parylene layer is disposed in the second moat to anchor the Parylene layer to the substrate, a column inlet in fluid communication with a first end of the column, a column outlet in fluid communication with a second end of the column opposite the first end, an injector intersecting the column on an inlet side of the column, and a detector disposed on the substrate on an outlet side of the column; placing the column inlet in fluid communication with an external source via an inlet port located on a first surface of a packaging jig, in the inlet port being in fluid communication with the column inlet through an internal jig conduit and an outlet port on a second surface of the packaging jig; establishing electronic communication with the detector on the substrate through a printed circuit board in contact with a side of the substrate opposite the packaging jig; injecting a sample including a plurality of sample components into the column using the injector; flowing a mobile phase through the column from the column inlet to the column outlet to separate the plurality of sample components; and detecting, using the detector, a changed property at a column outlet to reveal elution of at least one of the plurality of sample components.