Patent ID: 7361515

Claim:
A method for determining the presence or amount of analyte(s) in a sample, comprising: fluorescent labelling of at least one microparticle population, said microparticle population being labelled with at least one fluorescent dye serving as the dye producing a coding fluorescence and at least one fluorescent dye serving as the dye producing a reference fluorescence, binding and/or conjugating acceptor molecules to the microparticle population, immobilizing the microparticle population on a support, incubating the microparticle population with the sample, labelling the analyte(s) with at least one fluorescent dye producing a reporter fluorescence, exciting the fluorescent dyes, determining the presence or amount of analyte(s) in said sample by measuring and comparing the coding fluorescence of the microparticles with the reference fluorescence of the microparticles to identify the microparticle population, and measuring and comparing the reporter fluorescence of analyte(s) with the reference fluorescence of the microparticles to correct for failures caused by varying microparticle sizes within a population.