Patent ID: 8580271

Claim:
A composition for detection of the presence of anti-15-kDa IgG or IgM antibodies in a human suspected of Bartonella henselae infection, comprising: a) an isolated recombinant 15-kDa polypeptide, said isolated recombinant 15-kDa polypeptide having the amino acid sequence of SEQ ID NO: 2; and b) a support, wherein said isolated recombinant 15-kDa polypeptide is immobilized on said support and is prepared by a process, said process comprises the steps of: i) introducing a 15-kDa insert DNA having a nucleotide sequence of SEQ ID NO. 1 in an E. coli cell; ii) growing said E. coli cell that has said 15-kDa insert DNA in a culture medium; iii) adding 5-10 mg/mL glucose followed by IPTG in said culture medium; and iv) isolating said recombinant 15-kDa polypeptide from said culture medium.