Patent ID: 8309335

Claim:
A kit for generating 5′ ligation-tagged RNA from RNA in a sample, wherein the kit comprises an RNA 5′ polyphosphatase (RPP) comprising the sequence of SEQ ID NO 2 and at least one other component selected from the group consisting of: an RNA 5′ monophosphatase (RMP); an alkaline phosphatase (AP); a nucleic acid pyrophosphatase; a decapping enzyme; a capping enzyme; an RNA ligase; an RNA acceptor oligonucleotide; a poly(A) polymerase; a poly(U) polymerase; an RNA-dependent DNA polymerase (RT); a first-strand cDNA synthesis primer; an RNase H; a second-strand cDNA synthesis primer; an RNA polymerase (RNAP); 5′ exoribonuclease (Xrn); a polynucleotide kinase (PNK); and an RNA molecule that has a 5′ triphosphate or diphosphate group wherein the beta or gamma phosphate of said group is labeled.