Patent ID: 7977053

Claim:
A nucleic acid amplification method comprising: (a) providing a closed circular padlock probe molecule; a target nucleic acid molecule; a forward primer; a reverse primer; dNTPs; and a first DNA polymerase to form a reaction mixture; (b) incubating said reaction mixture at about 50° C. to about 70° C. for 1 minute to 30 minutes; (c) creating a multi-tailed complex by cascade rolling circle amplification wherein the multi-tailed complex has multiple primer binding sites, and wherein the multi-tailed complex is not a detectable amplification product; (d) activating a second DNA polymerase, wherein said second DNA polymerase is thermostable; and (e) thermocycling said multi-tailed complex with said second DNA polymerase, wherein said thermocycling said multi-tailed complex generates a detectable amplification product.