Patent ID: 8735573

Claim:
A process for the separation of bioactive compounds obtained from vegetable matter, the process comprising the steps of (a) contacting a plurality of bioactive compounds with a first polymer adsorbent under conditions allowing adsorption of at least one bioactive compound onto the first adsorbent while at least one bioactive compound is not adsorbed onto the first adsorbent; (b) collecting a solution comprising the at least one bioactive compound which has not adsorbed onto the first adsorbent; and (c) contacting the solution obtained in step (b) with a second polymer adsorbent under conditions allowing adsorption of at least one bioactive compound contained in the solution obtained in step (b) onto the second adsorbent, wherein the first polymer adsorbent is an acrylic polymer, the second polymer adsorbent is a polystyrene-divinyl benzene polymer, the plurality of bioactive compounds includes a flavanone glycoside and a limonoid glucoside, the flavanone glycoside is adsorbed onto the acrylic polymer, the limonoid glucoside is not adsorbed onto the acrylic polymer, and the limonoid glucoside is adsorbed onto the polystyrene-divinyl benzene polymer, thereby substantially separating the flavanone glycoside from the limonoid glucoside.