Patent ID: 8828717

Claim:
An isolated nucleic acid encoding a humanized COL-1 monoclonal antibody, wherein the humanized COL-1 monoclonal antibody comprises a heavy chain comprising the amino acid sequence of SEQ ID NO: 8 and a light chain comprising the amino acid sequence of SEQ ID NO: 4, but for: (a) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79 and a methionine substitution at position 81 of SEQ ID NO: 8; (b) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81 and a tyrosine substitution at position 27 of SEQ ID NO: 8; (c) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81 and a valine substitution at position 68 of SEQ ID NO: 8; (d) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81, at tyrosine substitution at position 27 and a valine substitution at position 68 of SEQ ID NO: 8; (e) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81 and a glutamine substitution at position 1 of SEQ ID NO: 8; (f) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81 and a lysine substitution at position 12 of SEQ ID NO: 8; (g) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81, a glutamine substitution at position 1 and a lysine substitution at position 12 of SEQ ID NO: 8; or (h) a valine substitution at position 20, an arginine substitution at position 38, a methionine substitution at position 48, an arginine substitution at position 67, a valine substitution at position 79, a methionine substitution at position 81, a glutamine substitution at position 1, a lysine substitution at position 12, a tyrosine substitution at position 27 and a valine substitution at position 68 of SEQ ID NO: 8, wherein the humanized COL-1 antibody retains binding affinity for carcinoembryonic antigen (CEA).