Patent ID: 8617846

Claim:
A method of providing a molecule for tumor recognition comprising: a. providing a recombinant recognition molecule which comprises a variable heavy chain and a variable light chain and which specifically binds to core 1 antigen, wherein the variable heavy chain comprises (i) a CDRH1 region that comprises the amino acid sequence of SEQ ID NO: 1, or a variant thereof having at least 70% homology with SEQ ID NO:1; (ii) a CDRH2 region that comprises the amino acid sequence of any one of SEQ ID NO: 2-3, or a variant thereof having at least 70% homology with SEQ ID NO:2 or SEQ ID NO:3; and (iii) a CDRH3 region comprises the amino acid sequence of any one of SEQ ID NO: 4-6, or a variant thereof having at least 70% homology to any one of SEQ ID NO:4-6; and the variable light chain comprises (i) a CDRL1 region that comprises the amino acid sequence of any one of SEQ ID NO: 7-9, or a variant thereof having at least 70% homology to any one of SEQ ID NOS:7-9; (ii) a CDRL2 region that comprises the amino acid sequence of any one of SEQ ID NO: 10-11, or a variant thereof having at least 70% homology to any one of SEQ ID NO:10-11; and a CDRL3 region that comprises the amino acid sequence of any one of SEQ ID NO: 12-13 or a variant thereof having at least 70% homology to any one of SEQ ID NO: 12-13, b. modifying the recombinant recognition molecule, and c. selecting a modified recombinant recognition molecule whose core 1 antigen specificity is retained.