Patent ID: 7254487

Claim:
A method for evaluating specificity of a drug comprising: a) comparing activity of a drug against its target pathway (D target ) in a biological sample and activity of said drug against at least one of its off-target pathways (D off-target ) in said biological sample, wherein said D target and D off-target are represented by quantities selected from the group consisting of ( i ) D target = R target , k ⁡ ( α target , t l ) ⁢ ⁢ and D off - target = R off - target , k ⁡ ( α off - target , t l ) , respectively ; ( ii ) D target = ∑ k ⁢ β k ⁢ R target , k ⁡ ( α target , t l ) ⁢ ⁢ and D off - target = ∑ k ⁢ β k ⁢ R off - target , k ⁡ ( α off - target , t l ) , respectively ; and ⁢ ( iii ) D target = C target ⁢ ⁢ and ⁢ ⁢ D off - target = C off - target , respectively ; wherein α target is a scaling constant of a scaling transformation of said target pathway, α off-target is a scaling constant of a scaling transformation of said at least one of its off-target pathways, R target, k (α target ,t l ) is a scaled response of cellular constituent k in said target pathway and R off-target, k (α off-target ,t l ) is a scaled response of cellular constituent k in said at least one of its off-target pathways, t l is a drug exposure level, β k is a constant for cellular constituent k, C target is the minimal level of said drug to achieve a threshold response in said target pathway, and C off-target is the minimal level of said drug to achieve a threshold response in said at least one of its off-target pathways; thereby evaluating specificity of said drug; and b) displaying or outputting a result of said comparing step a) to a user, a computer readable storage medium, a monitor, or a computer that is part of a network.