Patent ID: 7592179

Claim:
A method for preparing a five-part differential white blood cell control, said method comprising the following steps of: creating a mixed solution comprising concentrated whole blood cells, a hemolytic agent, a first amount of a first fixing agent, and an istonic solution, in which the concentrated whole blood cells comprise red blood cells and white blood cells, the isotonic solution is used at least for controlling a concentration of the concentrated whole blood cells, a concentration of the hemolytic agent, and a concentration of the first fixing agent, and the concentration of the first fixing agent is selected at least for preliminarily fixing the white blood cells without partially fixing at least some of the red blood cells to preserve a characteristic of the white blood cells, wherein the characteristic comprises a light scatting property, and adding a second amount of the first fixing agent and/or an amount of a second fixing agent to the mixed solution in order to reinforce fixation of the white blood cells.