Patent ID: 8048658

Claim:
A recombinant fungus of the Saccharomyces genus comprising heterologous genes encoding enzymes in a heterologous pathway for converting 1-deoxy-D-xylulose 5-phosphate (DXP) to isopentenyl diphosphate (IPP) or dimethylallyl diphosphate (DMAPP), the native metabolism of the microorganism being devoid of the pathway, such heterologous genes being genes encoding a functional 1-deoxy-D-xylulose 5-phosphate (DXP) synthase, a functional DXP-reductoisomerase, a functional (2-C-methyl-D-erythritol 4-phosphate (MEP) cytidylyltransferase, a functional 4-diphosphocytidyl-2-C-methyl-D-erythritol (CDP-ME) kinase, a functional 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) synthase, a functional MECDP reductase and a functional 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) reductase, said microorganism further comprising a heterologous gene encoding a functional terpene synthase, with all heterologous genes integrated in the genome of the fungus, which is the Saccharomyces cerevisiae strain YIP-DV-02 having deposit number DSM 17900.