Patent ID: 8282923

Claim:
A mixture of two or more isolated inhibitory antibodies against factor VIII, said mixture comprising at least one antibody selected from the group consisting of: (i) an inhibitory antibody against factor VIII, or a fragnment thereof, comprising the immunoglobulin variable light chain CDR1, CDR2, and CDR3 regions depicted in SEQ ID NO:36, SEQ ID NO:37, and SEQ ID NO:38, respectively, and comprising the immunoglobulin variable heavy chain CDR1, CDR2, and CDR3 regions depicted in SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35, respectively, wherein the glycosylation site at positions 3 and/or 5 in the CDR1 region of the heavy chain is mutated; (ii) an inhibitory antibody against factor VIII, or a fragnment thereof, comprising the immunoglobulin variable light chain CDR1, CDR2, and CDR3 regions depicted in SEQ ID NO:36, SEQ ID NO:37, and SEQ ID NO:38, respectively, and comprising the immunoglobulin variable heavy chain CDR1, CDR2, and CDR3 regions depicted in SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35, respectively, wherein Asn at position 3 in the CDR1 region of the variable heavy chain is deglycosylated; and (iii) an inhibitory antibody against factor VIII, or a fragnment thereof, comprising the immunoglobulin variable light chain the CDR1, CDR2, and CDR3 regions depicted in SEQ ID NO:36, SEQ ID NO:37, and SEQ ID NO:38, respectively, and comprising the immunoglobulin variable heavy chain the CDR1, CDR2, and CDR3 regions depicted in SEQ ID NO:33, SEQ ID NO:34, and SEQ ID NO:35, respectively, wherein said antibody is produced in CHO cells.