Patent ID: 8075925

Claim:
A method for production of a canola protein isolate consisting predominantly of the 2S canola protein, which comprises: extracting canola oil seed meal with an aqueous salt solution having an ionic strength of at least about 0.05 and a pH of about 5 to about 6.8 at an elevated temperature of about 80° to about 100° C. to preferentially extract the 2S protein from the canola oil seed meal in preference to the 7S and 12S proteins and to obtain a canola protein extract solution containing predominantly 2S protein, separating the canola protein extract solution from residual canola oil seed meal, and recovering from the canola protein extract solution a canola protein isolate having a protein content of at least about 90 wt % (N×6.25) and consisting predominantly of the 2S canola protein, wherein said recovering of the canola protein isolate is effected by concentrating the canola protein extract solution, wherein the concentrated canola protein extract solution is subjected to a heat treatment step so as to form a precipitate to decrease the proportion of the 7S and 12S protein in said solution by at least about 50 wt %, and wherein the precipitate is separated from the solution.