Patent ID: 7344885

Claim:
A recombinant expression cassette comprising a promoter that is functional in plants operably linked with a coding sequence having a stop codon, the coding sequence being operably linked with a non-plant 3′ termination sequence, wherein the non-plant termination sequence is heterologous to the coding sequence and comprises: i. a cleavage site including a nucleotide sequence YA defining a position of endonucleolytic cleavage and subsequent 3′ polyadenylation; ii. a positioning element of 6 nucleotides located between 10 nucleotides and 40 nucleotides 5′ of the cleavage site and with at least 4 out of 6 nucleotides being adenine; iii. an upstream element that (a) is located between 1 nucleotide and 250 nucleotides 5′ of the positioning element; and (b) comprises TAYRTA or two or more repeats of the TA, TG, or TA and TG where the repeats are separated by 0 to 10 nucleotides; wherein the non-plant 3′ termination sequence is a fungal 3′ termination sequence.