Patent ID: 8530165

Claim:
A nucleic acid detection method comprising: (a) performing a nucleic acid extension reaction by mixing: a nucleic acid sample; an analyte nucleotide-identification primer being a polynucleotide which comprises: a nucleotide sequence being homologous or complementary to a partial region containing an analyte nucleotide on a target nucleotide sequence, and having an insertion site created by inserting one to three nucleotides or a deletion site created by deleting one to three nucleotides, wherein the insertion site and deletion site are on the 5′ side of the nucleotide corresponding to the analyte nucleotide; and a polymerase, wherein if the target nucleotide sequence that contains the analyte nucleotide is present in the mixture, an extension product is formed and if no analyte nucleotide is present in the mixture, no extension product is formed; and (b) determining if the extension product is formed in the mixture of (a), which contains a nucleic acid having the target nucleotide sequence comprising one or more analyte nucleotide(s), thereby determining whether or not the extension product is created by the extension reaction.