Patent ID: 7582744

Claim:
An isolated oligonucleotide agent, comprising a nucleotide sequence consisting of from 12 to 23 nucleotides in length sufficiently complementary to a microRNA target sequence of about 12 to 23 nucleotides, wherein the nucleotide sequence of the oligonucleotide agent differs by no more than 1 or 2 nucleotides from full complementary to the microRNA target sequence and wherein said oligonucleotide agent has the structure (I) (5′) QxQz 1 (Qy) n Qz 2 Qz 3 Qz 4 Q-L (3′) (I) wherein Q is a 2′-O-methyl modified nucleoside; x, z 1 , z 2 , z 3 , and z 4 are all One of A and B is S while the other is O; n=6-17; L is wherein: X is N(CO)R 7 , or NR 7 ; each of R 1 , R 3 and R 9 , is, independently, H, OH, or —CH 2 OR b provided that at least one of R 1 , R 3 , or R 9 is OH and at least one of R 1 , R 3 or R 9 is —CH 2 OR b ; R 7 is C 1 -C 20 alkyl substituted with NR c R d or NHC(O)R d ; R c is H or C 1 -C 6 alkyl; R d is a carbohydrate radical; or a steroid radical, which is optionally tethered to at least one carbohydrate radical; and R b is one of E and F is S while the other is O.