Patent ID: 6953686

Claim:
A method for purifying plasmid DNA from a mixture containing plasmid DNA and at least one host cell impurity comprising the following steps: (a) forming a solution comprising a salt with said mixture wherein said solution has a salt concentration in the range of about 2M to 4M to allow selective binding of said at least one host cell impurity to a chromatography support comprising a hydrophobic pendent group; (b) contacting said solution containing plasmid DNA with said support at a salt concentration whereby said at least one impurity binds to said hydrophobic pendent group to form a complex; and (c) collecting unbound plasmid DNA from said complex without further chromatographic separation; wherein said method is conducted in the absence of organic solvents, detergents, glycols, hexamine cobalt, spermidine, and polyvinylpyrollidone.