Patent ID: 8070827

Claim:
A method for use of a double-structured tissue implant for treatment of a tissue defect, said method comprising steps: a. preparing a double-structured tissue implant comprising of a primary scaffold and a secondary scaffold incorporated into said first scaffold; b. preparing said tissue defect for implantation; c. precutting said double-structured tissue implant into a size of said defect; d. coating said defect with a tissue adhesive; e. implanting said double-structured tissue implant into said defect wherein said implant is rehydrated with a physiologically acceptable solution or buffer before or after implantation; and f. covering said implant with a tissue adhesive, wherein said primary scaffold of said double-structured tissue implant is a porous structure prepared from a biocompatible collagenous material selected from the group consisting of Type I collagen, Type II collagen, Type IV collagen, gelatin, collagen containing agarose, collagen containing hyaluronan, collagen containing proteoglycan, collagen containing glycosaminoglycan, collagen containing glucosamine, collagen containing galactosamine, collagen containing glycoprotein, collagen containing fibronectin, collagen containing laminin, collagen containing polylactic acid, collagen containing polyglycolic acid, collagen containing polyamino acid, collagen containing polycaprolactone, alpha 1 Type I peptide, alpha 2 Type I peptide, 2 alpha 1 Type I peptide, 1 alpha 2 Type I peptide, 3 alpha 1 Type II peptide, and a combination thereof, wherein said primary scaffold has substantially homogeneous porosity wherein at least 85% of pores have sizes of about 300Â±100 Î¼m, wherein said secondary scaffold of said double-structured tissue implant is prepared from a composition comprising a soluble collagen or collagen-containing compound and a non-ionic surfactant and introduced into said pores of said primary scaffold, stabilized by precipitation or gelling, lyophilized and dehydrothermally treated; wherein said double-structured tissue implant is rehydrated with a physiologically acceptable solution or buffer and has a rehydration and wettability time of about two seconds; and wherein said rehydrated double-structured tissue implant is resistant to dissolution, change in size and shape and maintains its collagen retention of about 99%, has pH between 7.7 and 7.8 and osmolality between 317 and 356 mOsm/kg.