Patent ID: 8653245

Claim:
A method for purifying Apolipoprotein A-I (ApoA-I) and Alpha-1-Antitrypsin (AAT) from a single starting human plasma fraction containing both proteins comprising: (i) treating a starting human plasma fraction comprising ApoA-I and AAT to separate an ApoA-I containing fraction from an AAT containing fraction, comprising (a) treating the starting human plasma fraction which is used as the starting material such that ApoA-I and AAT are solubilized; (b) adding an ApoA-I binding agent and ensuring an appropriate pH so that ApoA-I binds to the added ApoA-I binding agent, wherein the binding agent is fumed silica; (c) separating ApoA-I bound to said fumed silica from the solution containing AAT; (d) eluting ApoA-I from said fumed silica; and (ii) separately purifying ApoA-I and AAT in one or more processing steps.