Patent ID: 7920972

Claim:
A method of conducting a (5,3) dimensional (D) [HN{ N , CO }{ C αβ C α }] G-matrix Fourier transformation (GFT) nuclear magnetic resonance (NMR) experiment, said method comprising: providing a sample, wherein said sample is a protein molecule having two consecutive amino acid residues, i−1 and i, and the chemical shift values for the following nuclei are measured: (1) α- and β-carbons of amino acid residues i and i−1, 13 C α/β i/i−1 ; (2) a polypeptide backbone amide nitrogen of amino acid residue i, 15 N i ; (3) a polypeptide backbone carbonyl carbon of amino acid residue i−1, 13 C′ i−1 ; and (4) a polypeptide backbone amide proton of amino acid residue i, 1 H N i ; applying radiofrequency pulses for a first 5D FT NMR experiment to the sample; using a spectrometer; selecting a first set of 2 indirect chemical shift evolution periods of the 5D FT NMR experiment, 13 C α/β i/i−1 and 13 C α i/i−1 ; jointly sampling the first set of 2 indirect chemical shift evolution periods in a first indirect time domain dimension, t 1 ( 13 C α/β i/i−1 , 13 C α i/i−1 ), whereby chemical shift evolution periods which provide sequential connectivities and spectral resolution are subjected to the joint sampling in the first indirect time dimension; selecting a second set of 2 indirect chemical shift evolution periods of the 5D FT NMR experiment, 15 N i and 13 C′ i−1 ; jointly sampling the second set of 2 indirect chemical shift evolution periods in a second indirect time domain dimension, t 2 ( 15 N i , 13 C′ i−1 ), whereby chemical shift evolution periods which serve to provide spectral resolution are subjected to separate joint sampling in the second indirect time dimension; independently cosine and sine modulating NMR signals detected in a direct dimension to generate 3D basic NMR spectra comprising frequency domain signals having a chemical shift multiplet with 4 components resulting from each of 2 chemical shift doublet components in a first frequency domain dimension, ω 1 ( 13 C α/β , 13 C α ), giving rise to 2 chemical shift doublet components in a second frequency domain dimension, ω 2 ( 15 N, 13 C′), thereby enabling phase-sensitive sampling of all 4 indirect chemical shift evolution periods; and transforming the 3D basic NMR spectra into 3D phase-sensitively edited basic NMR spectra, wherein the 4 chemical shift multiplet components of the 3D basic NMR spectra are edited to yield 3D phase-sensitively edited basic NMR spectra having individual chemical shift multiplet components.