Patent ID: 8341877

Claim:
A method of producing a biomass comprising: a) dispensing a biomass culture medium in a photobioreactor, the photobioreactor comprising: i) a cavity defined by: 1) a substantially V-shaped base comprising two base walls, said base walls meeting proximate to an axis defining an interior angle, each base wall comprising: A) a sloped portion and a substantially vertical portion; B) a proximal end and a distal end; and C) a length extending along said axis and a width extending perpendicular to said axis; 2) a proximal side wall adjacent to said proximal end; 3) a distal side wall adjacent to said distal end; ii) at least one gas delivery system disposed within said cavity and extending parallel to said axis; and iii) at least one carbon dioxide delivery system disposed within said cavity and extending parallel to said axis; b) supplying a gas through said gas delivery system, producing bubbles having diameters between about 1 and about 3 mm; and c) supplying carbon dioxide through said carbon dioxide delivery system, producing bubbles having diameters between about 0.001 and about 500 microns.