Patent ID: 8637292

Claim:
An isolated polypeptide having feruloyl esterase activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of amino acids 21 to 520 of SEQ ID NO: 2; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence that hybridizes under at least very high stringency conditions with (i) the nucleotide sequence of nucleotides 61 to 1560 of SEQ ID NO: 1, (ii) a cDNA sequence contained in the nucleotide sequence of nucleotides 61 to 1560 of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide following standard Southern blotting procedures for 12 to 24 hours optimally, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.