Patent ID: 8709767

Claim:
A process for the enzymatic reduction of a compound (1), wherein the C═C bond of the compound (1) according to formula (1) is stereoselectively hydrogenated in the presence of an enoate-reductase and an oxidizable co-substrate which is a compound having a chemical structure according to formula (1) in a system which is free of NAD(P)H, in which A is a ketone radical (—CRO), an aldehyde radical (—CHO), a carboxyl radical (—COOR), with R =H or optionally substituted C 1 -C 6 -alkyl radical, R 1 , R 2 and R 3 are independently of one another H, —O—C 1 -C 6 -alkyl, C 1 -C 6 -alkyl, which can be substituted, C 2 -C 6 -alkenyl, carboxyl, or an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or R l is linked to R 3 so as to become part of a 4-8-membered cycle, or R l is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R 1 , R 2 and R 3 may not be identical; wherein the enoate reductase is selected from: (i) an enoate reductase comprising at least one of the polypeptide sequences SEQ ID NO: 3 or 4; or (ii) an enoate reductase comprising a polypeptide sequence which has at least 95% sequence identity with SEQ ID NO: 3.