Patent ID: 8232382

Claim:
A kit for determining sex of a bird, comprising: a first oligonucleotide set including (i) a first oligonucleotide having the nucleotide sequence of SEQ ID NO: 5 or its complementary sequence; (ii) a second oligonucleotide having the nucleotide sequence of SEQ ID NO: 6 or its complementary sequence; (iii) a third oligonucleotide having the nucleotide sequence of SEQ ID NO:7 or its complementary sequence; (iv) a fourth oligonucleotide having the nucleotide sequence of SEQ ID NO: 8 or its complementary sequence; (v) a fifth oligonucleotide having the nucleotide sequence of SEQ ID NO: 9 or its complementary sequence; (vi) a sixth oligonucleotide having the nucleotide sequence of SEQ ID NO: 10 or its complementary sequence; and (vii) a seventh oligonucleotide having SEQ ID NO: 11 or its complementary sequence; and a second oligonucleotide set including (viii) an eighth oligonucleotide having the nucleotide sequence of SEQ ID NO: 1 or its complementary sequences; and (ix) a ninth oligonucleotide having the nucleotide sequence of SEQ ID NO: 2 or its complementary sequence; wherein each of the oligonucleotides in the first and second sets has up to 60 nucleotides, including an optional linker sequence at the 3′ end or 5′ end of the oligonucleotide.