Patent ID: 8597910

Claim:
A recombinant method of producing a functional human von Willebrand Factor protein that can be recognized by anti-von Willebrand Factor antibodies, wherein the method comprises the steps of (1) culturing a host cell from a mammalian cell line, wherein the host cell is transformed with an expression vector which comprises (a) the von Willebrand Factor-encoding nucleotide sequences of (i) pVWH33 (ATCC No. 53090), (ii) pVWH5 (ATCC No. 53088), and (iii) the sequence of nucleotides 9 through 587 of Table 1, wherein the sequences are combined 5′3′ in the order (i), (ii), and (iii), and (b) heterologous DNA effective to cause expression of the von Willebrand Factor-encoding nucleotide sequences, wherein the culturing is performed in a culture medium and under conditions permitting the transformed host cell to express functional von Willebrand Factor protein that can be recognized by anti-von Willebrand Factor antibodies, and (2) recovering the functional von Willebrand Factor protein from step 1.