Patent ID: 8637250

Claim:
A method for detecting the presence or absence of a polynucleotide target in a sample, comprising: a. providing a coated, solid support comprising (i) a solid support, (ii) a coating layer comprising at least one polycationic polymer, and (iii) a plurality of target-specific probes attached to the coating layer; b. contacting the coated, solid support with a sample suspected of having a polynucleotide target and a reaction medium comprising helicase, DNA polymerase, target-specific DNA primers, and dNTPs, wherein the reaction medium has a monovalent cation concentration between about 10 mM and about 100 mM; c. heating the sample and reaction medium to produce isothermally amplified polynucleotide targets that are simultaneously captured by target-specific probes on the coated, solid support; d. detecting the presence or absence of the polynucleotide target from amplified polynucleotide targets bound to the target-specific probes.