Patent ID: 6943019

Claim:
A recombinant host cell containing (a) a first nucleic acid molecule comprising a parvovirus P5 promoter in its native configuration with response to wild-type AAV, an AAV rep gene sequence, an AAV cap gene sequence, and a space of sufficient size to reduce expression of the rep78 and rep68 gene products relative to other rep gene products, wherein said spacer is heterologous to said promoter and is interposed between said promoter and the start site of the rep gene; (b) a second nucleic acid molecule comprising a minigene comprising a transgene flanked by AAV inverted terminal repeats (ITRs) and under the control of regulatory sequences directing expression thereof in a host cell; and (c) helper functions essential to the replication and packaging of rAAV; wherein the nucleic acid of (a) or (b) is selected from the group consisting of a plasmid, a recombinant virus, and an episome.