Patent ID: 8632963

Claim:
A composition for increasing the permeability of cell walls and cell membranes of Mycobacterium sp., said composition comprising: GuSCN (guanidine thiocyanate) at a concentration of approximately 2.0 to 3.3 M, Tris-HCL at a concentration of approximately 10.0 to 100.0 mM and at a pH of approximately 7.0 to 9.0, EDTA at a concentration of approximately 5 to 50 mM, IGEPAL (octylphenoxy poly(ethyleneoxy)ethanol) at a concentration of approximately 0.1 to 2.0 percent (v/v), acetic acid at a concentration of approximately 1.0 to 10 percent (v/v), methanol at a concentration of approximately 20 to 50 percent (v/v), sodium cholate at a concentration of approximately 0.02 to 2.5 percent (w/v) and, sodium deoxycholate at a concentration of approximately 0.02 to 2.5 percent (w/v), whererin said composition is effective in permeabilizing the cell walls and cell membranes of Mycobacterium sp. but not effective in lysing Mycobacterium sp.