Patent ID: 7579170

Claim:
A method for preparing a monoclonal antibody capable of specifically activating effector cells expressing FcγRIII, wherein the method comprises: purifying a monoclonal antibody obtained from a clone originated from a cell line selected from hybridomas and animal or human cell lines transfected with a vector comprising a gene encoding said antibody; then adding the antibody to a reaction mixture comprising: target cells for said antibody, effector cells comprising cells expressing FcγRIII and polyvalent IgGs; then determining an FcγRIII-type ADCC activity level as a lysis percentage of the target cells then selecting the monoclonal antibody, which activates the effector cells and causes an FcγRIII-type ADCC activity level greater than 60% and adding an anti-FcγRIII antibody in the reaction mixture in order to confirm the FcγRIII-type ADCC activity level due to the selected monoclonal antibody.