Patent ID: 8703431

Claim:
A method for the in vitro assessment of the skin sensitising potential of a test compound, comprising the steps of: a) contacting a test compound with an isolated polypeptide comprising a sequence selected from the group consisting of the sequences SEQ ID NOS: 1, 2, 3 and 4 and derivatives thereof; b) measuring any binding of said test compound with said polypeptide wherein the derivatives thereof are isolated polypeptides comprising one or more conservative sequence mutations, wherein the conservative sequence mutations do not alter pKa values of cysteine thiol groups and/or lysine residues, wherein the conservative sequence mutations do not modify activity of the isolated polypeptide, and wherein each of the derivatives thereof has at least 95% sequence identity with one of the group consisting of the sequences SEQ ID NOS: 1, 2, 3 and 4.