Patent ID: 7288378

Claim:
A method, comprising: (a) incubating a reaction mixture comprising: (i) a sample nucleic acid obtained from a biological sample suspected of containing a TIGR nucleic acid sequence, (ii) a nucleic acid polymerase, (iii) one or more extension primers that specifically bind to said TIGR nucleic acid sequence if present, and that, when extended by one nucleotide at the 3′ end, comprise a nucleotide indicative of one or more preselected polymorphisms in said TIGR nucleic acid sequence, and (iv) one or more labeled ddNTPs, under conditions such that, in the presence of said TIGR nucleic acid sequence, said extension primer(s) are distinctively labeled by addition of one of said labeled ddNTP(s) to the 3′-end of said detection primer, to generate a labeled nucleic acid corresponding to one of said preselected polymorphism(s); and (b) detecting a signal from said labeled nucleic acid, wherein said signal is related to a TIGR genotype present in said sample; wherein said sample nucleic acid is obtained by amplification of nucleic acid in said biological sample; wherein nucleic acid in said biological sample is amplified by a polymerase chain reaction; and wherein nucleic acid in said sample is amplified using one or more amplification primer sequences selected from the group consisting of SEQ ID NOS:5-8.