Patent ID: 8551717

Claim:
A method for determining SUMOylation of a molecule of interest comprising the steps of a) providing a first construct comprising Ubc9 or a homologue thereof having Ubc9 enzymatic activity and fused therewith a first moiety of a binding pair allowing for specific binding with the second moiety of said binding pair; b) providing the molecule of interest fused with said second moiety of the binding pair which specifically interacts with the first moiety of a binding pair fused with Ubc9 or a homologue thereof having Ubc9 enzymatic activity; c) optionally providing SUMO molecules being marked with a marker molecule; d) allowing binding of said first moiety of a binding pair with said second moiety of said binding pair; e) optionally, inducing binding between the two moieties of the binding pair in step d) by providing a molecule inducing formation of a complex of the first moiety of said binding pair and the second moiety of said binding pair; f) determining conjugation of SUMO molecule(s) with the molecule of interest fused with the second moiety of said binding pair.