Patent ID: 7785872

Claim:
A nucleic acid molecule comprising a nucleic acid sequence for mediating gene expression, protein expression, and protein secretion, wherein the nucleic acid sequence comprises a sequence encoding a secretory protein, the nucleic acid sequence having a formula: [A(A′) x B] n wherein A represents a sequence encoding a promoter operably linked to a sequence encoding a signal peptide; A′ represents a sequence encoding a pro-region of yeast α-mating factor (SEQ ID NO: 1); B represents the sequence encoding the secretory protein; x represents 0-1; n represents 1-20; provided that if x is 0 then n is not 1; wherein the sequence encoding the signal peptide, SEQ ID NO:1 and the sequence encoding the secretory protein are operably linked and under control of the promoter, said signal peptide is selected from a modified signal peptide of α-amylase, wherein Serine at position 8 of the signal peptide of α-amylase is replaced by Leucine (SEQ ID NO: 15) or a modification of SEQ ID NO:15 wherein position 8 comprises an amino acid selected from Isoleucine, Valine, Alanine, Glycine or Phenylalanine; and said protein is Alternaria alternata Alt a 1 allergen, Alternaria alternata rAlt a 2 allergen, Aspergillus awamori glucoamylase, Aspergillus awamori glucoamylase catalytic domain, Aspergillus fumigatus Asp f 2 allergen, Aspergillus fumigatus catalase L, Aspergillus fumigatus dipeptidyl peptidase IV (DPP IV), Aspergillus fumigatus dipeptidyl peptidase V (DPP V), Aspergillus fumigatus phytase, Aspergillus giganteus alpha-sarcin ribotoxin, Aspergillus niger 13-glucosidase, Aspergillus niger phytase (phyA), Candida guilliermondii xylose reductase gene (xylI), Aspergillus niger endo-β-1,4-xylanase, Candida rugosa lipase 1 (CRL), Fusarium solani pectate lyase (pelC), Fusarium solani pectate lyase (pelD), Geotrichum candidum lipase isoenzymes, Phytophthora cryptogea β-cyptogein, Rhizopus oryzae lipase, Saccharomyces cerevisiae invertase, Saccharomyces cerevisiae Ktr1p, Saccharomyces cerevisiae (a-1,2-mannosyltransferase), Schizophyllum commune vitamin B2-aldehyde-forming enzyme, Trametes versicolor (white rot fungus) laccase (lccI), Trichoderma harzianum β-(1-6)-glucanase, Candida albicans MNT1 mannosyltransferase, Candida antarctica Lipase B, Candida rugosa LIP2 lipase, Candida rugosa lip4 lipase, Kurtzmanomyces sp. 1-11 lipase, Metarhizium anisopliae chymotrypsin, Mucor pusillus rennin, Neurospora crassa NOP-1 , Neocallimastix patriciarum cellulase 6A- Candida Antarctica lipase B fusion protein, Phanerochaete chrysosporium glyoxal oxidase, Phanerochaete chrysosporium cellobiose dehydrogenase, Phanerochaete chrysosporium β-glucosidase, Phaeseolus vulgaris aspartic protease, Barley glucosidase, Pleurotus sajor - caju laccase isozyme, Pycnoporus cinnabarinus 1-937 laccase, Saccharomyces cerevisiae Hsp70 , Saccharomyces cerevisiae Kex 2p, Trichoderma reesei cellobiohydrolyase I, Trichoderma reesei cellobiohydrolyase Cel7A or Trichoderma reesei 1,2-a-kD-mannosidase.