Patent ID: 7678547

Claim:
A method for determining a characteristic parameter of an analyte, wherein the characteristic parameter of the analyte is independent of a flow velocity of the analyte, but a measurement of the characteristic parameter of the analyte is capable of being influenced by or dependent on the velocity of the analyte, said method comprising: (a) transporting a fluid medium comprising the analyte from a first position to a second position of a fluid flow channel of a fluidic device; (b) measuring the characteristic parameter of the analyte within the fluid flow channel at a plurality of different detection zones separated along a flow path of the analyte in between the first and the second positions; (c) determining a velocity dependence of the measurement of the characteristic parameter; and (d) determining the characteristic parameter of the analyte independent of the flow velocity of the analyte by using the measurements of the characteristic parameter of step (b) and normalizing the measurements of the characteristic parameter by substantially eliminating the velocity dependence of the measurements of the characteristic parameter.