Patent ID: 8518667

Claim:
A fusion DNA construct encoding a fusion polypeptide comprising in operable linkage from the 5′ end of said construct, a promoter, a first DNA molecule, which is different from any one of the second, the third, and the fourth DNA molecules, encoding a signal sequence, a second DNA molecule, which is different from any one of the first, the third, and the fourth DNA molecules, encoding a carrier protein, a third DNA molecule, which is different from any one of the first, the second, and the fourth DNA molecules, encoding a KEX2 region, said region comprising a KEX2 site (B 1 B 2 ) and a KEX2 site pre-sequence X4X3X2X1 immediately 5′ to the KEX2 site, wherein X4 is V, S, N, L, or K, X3 is A, V, D, W, E, or P; X2 is V, I, L or F; and X1 is E, S, T or Y, wherein the pre-sequence is not NVIS (SEQ ID NO:85; and a fourth DNA molecule, which is different from any one of the first, the second, and the third DNA molecules, encoding a protein.