Patent ID: 7820422

Claim:
A method of producing an oligosaccharide comprising Neu5Acα-3Galβ-4Glc, the method comprising: (a) obtaining a Lac Y+ and NanT+ Escherichia coli microorganism; and (b) culturing the microorganism in a culture medium comprising precursors consisting of lactose and sialylic acid, wherein the microorganism comprises a heterologous gene encoding a bifunctional Campylobacter jejuni sialyltransferase polypeptide which catalyzes the transfer of a sialyl moiety from an activated sialic acid molecule to the lactose to form Neu5Acα-3Galβ-4Glc, and wherein the Lac Y+ and NanT+ microorganism allows active transport of lactose and sialylic acid.