Patent ID: 6866782

Claim:
A process for the purification of the recombinant alpha-2b interferon, rIFNα-2b, that comprises charging a proteic mixture coming from the manufacturing by fermentation of the rIFNα-2b added with a 1M solution of sodium acetate and brought to pH 5.5 with acetic acid, on a column filled with strong cationic exchange resin conditioned at pH 5.5 by means of a 20 mM solution of sodium acetate so that between 6 and 8 mg of protein are present for each ml of stationary phase, in submitting the column to two washing cycles, first with a buffer solution at pH 6.1 at a concentration between 5 and 15 mM, then with the same buffer solution added with 2 mM of potassium chloride and lastly in eluting the pure rIFNα-2b from the columns by using a buffer solution at pH 6.1 at a concentration between 5 and 15 mM containing potassium chloride at a concentration comprised between 15 and 25 mM.