Patent ID: 7700750

Claim:
A kit for characterizing a target sequence for the presence or absence of Connexin 26 allele 30ΔG, wherein said kit comprises first and second oligonucleotides configured to form an invasive cleavage structure when in combination with said target sequence, wherein said first oligonucleotide comprises a 5′ portion and a 3′ portion, wherein said 3′ portion of said first oligonucleotide comprises the sequence 5′ CCCAGGATCGTCTG 3′ (SEQ ID NO:15) or 5′ CCCCAGGATCGTCT 3′ (SEQ ID NO:17) configured to hybridize to said target sequence, and wherein said 5′ portion of said first oligonucleotide is configured to not hybridize to said target sequence, and wherein said second oligonucleotide comprises a 5′ portion and a 3′ portion, wherein said 5′ portion of said second oligonucleotide comprises the sequence 5′ TGCTGGTGGAGTGTTTGTTCACACC 3′ (SEQ ID NO:16) configured to hybridize to said target sequence, and wherein said 3′ portion of said second oligonucleotide is configured to not hybridize to said target sequence.