Patent ID: 8394590

Claim:
A method for sorting cells comprising: combining a sample having a plurality of target cells with a plurality of capture agent sets, each capture agent set comprising a plurality of capture agents comprising a tetramer of an antigen-presenting MHC attached to an optimized streptavidin scaffold molecule and an encoding polynucleotide attached to the scaffold molecule, wherein the MHC specifically binds to a predetermined target cell and the encoding polynucleotide specifically binds to a complementary substrate polynucleotide and wherein each capture agent within a capture agent set binds the same target cells and binds the same complementary substrate polynucleotides; forming target cell complexes of capture agents bound to the target cells; combining the target cell complexes with a plurality of complementary substrate polynucleotide sets attached to a substrate; and hybridizing the encoding polynucleotides to complementary substrate polynucleotides to sort target cell complexes.