Patent ID: 7968288

Claim:
A method of obtaining mRNA from a leukocyte lysate comprising: obtaining whole blood; preparing a plurality of sample wells, each of the sample wells comprising a filter that traps leukocytes in the filter and allows erythrocytes and blood components other than leukocytes in the whole blood to be removed from the filter; adding an anticoagulant to the whole blood; adding the whole blood to the sample wells; removing erythrocytes and blood components other than leukocytes in the whole blood from the filter; lysing the leukocytes in the samples wells by applying lysis buffer comprising a guanidinium thiocyanate at a concentration in the range of about 1.4M to 1.75M into the sample wells and obtaining a leukocyte lysate comprising mRNA in each of the sample wells; transferring said leukocyte lysate from each of the sample wells into its corresponding mRNA capture zone in which oligo(dT) is immobilized; and obtaining said mRNA from said leukocyte lysate by annealing poly-A mRNA in the leukocyte lysate to the oligo(dT).