Patent ID: 7320878

Claim:
A method for determining the inhibitory potency of an HIV inhibitor in a biological sample, comprising: i) providing a biological sample comprising said HIV inhibitor; ii) providing HIV protease; iii) providing a substrate for HIV protease R-E(EDANS)-S-Q-N-Y-P-I-V-Q-K(DABCY-L)-R-OH (SEQ ID NO: 10); iv) separating HIV inhibitor bound to proteins in the biological sample from unbound HIV inhibitor by gel filtration, filters, filter papers, special microtiter plates, dialysis, heating, precipitation, centrifugation, or antibodies or by combinations thereof; v) adding the resulting biological sample from which bound HIV inhibitor has been separated, the HIV protease and the HIV protease substrate to a container; vi) determining a signal; and vii) relating the signal of vi) to a reference standard curve prepared with at least one reference.