Patent ID: 8450441

Claim:
A production method of an N-linked sialo-glycan-containing polymer comprising: forming an amide bond between (i) a linker compound having an amino group and a carboxyl group on each end of the compound respectively and (ii) a 1-amino sugar in which a 1 position of a reducing terminal of the sugar is aminated, thereby obtaining an N-linked asialo-glycan having an asialo-glycan part on a non-reducing terminal of a sugar; obtaining an N-linked sialo-glycan in which a sialo-glycan is synthesized to the non-reducing terminal side by sialylation of the N-linked asialo-glycan; and synthesizing a sialo-glycan-containing polymer by condensing the N-linked sialo-glycan to a carboxyl group of a γ-polyglutamic acid via the amino group of the linker, which is linked to a reducing terminal of the N-linked sialo-glycan, wherein the linker is an w-amino acid comprising a hydrocarbon with a carbon number of 1-30 that is an alkyl group, an alkenyl group, a cycloalkyl group, an aryl group, an aralkyl group, a cycloalkyl substituted alkyl group or an amide linked hydrocarbon of two or more of these, and wherein the γ-polyglutamic acid is dissolved in a mixed solution comprising (i) water or a buffer and (ii) an organic solvent, and the N-linked sialo-glycan is condensed with the γ-polyglutamic acid after activating the γ-polyglutamic acid using an active esterification agent of a carboxyl group, condensing agent, and/or additive.