Patent ID: 8105810

Claim:
A method for producing a peroxycarboxylic acid comprising (a) providing a first component comprising: (i) a carboxylic acid ester substrate; (ii) an enzyme catalyst having perhydrolysis activity, wherein said enzyme catalyst comprises an enzyme having a carbohydrate esterase family 7 (CE-7) signature motif that aligns with SEQ ID NO: 2 using CLUSTALW, said signature motif comprising: (1) an RGQ motif at amino acid positions aligning with 118-120 of SEQ ID NO:2; (2) a GXSQG motif at amino acid positions aligning with 179-183 of SEQ ID NO:2; and (3) an HE motif at amino acid positions aligning with 298-299 of SEQ ID NO:2; said enzyme comprising at least 90% amino acid identity to SEQ ID NO: 2, 14, 16, 73 or 74; and (iii) at least one cosolvent comprising an organic solvent having a log P of less than about 2, wherein log P is defined as the logarithm of the partition coefficient of a substance between octanol and water, expressed as P=[solute] octanol /[solute] water and wherein the at least one cosolvent is not a substrate for said enzyme catalyst; wherein said first component is a substantially non-aqueous formulation of (i)-(iii); (b) providing a second component comprising a source of peroxygen in water; and (c) combining said first component and said second component to form an aqueous reaction formulation, wherein said cosolvent solubilizes the carboxylic acid ester substrate in the aqueous reaction formulation without substantial loss of perhydrolytic activity of the enzyme catalyst and whereby peroxycarboxylic acid is produced.