Patent ID: 7083971

Claim:
A composition comprising: a vector for capture, cloning, manipulation, production and delivery of large nucleic acids to a yeast target cell comprising: (a) a first arm having in sequential order, a first selectable marker, a first cyclization element, and a first segment homologous to the 5′ terminus of a target polynucleotide; and (b) a second arm having in sequential order, a second selectable marker, a second cyclization element, and a second segment homologous to the 3′ terminus of the target polynucleotide, wherein said vector comprises a polynucleotide sequence homologous to a yeast target polynucleotide sequence for homologous recombination with the target polynucleotide in said yeast target cell, and wherein the target polynucleotide is a polynucleotide of a virus, and said virus is selected from the group consisting of adenovirus, adeno-associated virus, pox virus, papova virus and herpes virus.