Patent ID: 7033814

Claim:
A method for preparing yeast with improved biotin-productivity, comprising the steps of: (a) providing an integrating plasmid comprising: (i) a promoter sequence that is functional in yeast, and which is operably linked to a polynucleotide sequence encoding Candida utilis biotin synthase, wherein the polynucleotide sequence encoding Candida utilis biotin synthase comprises the nucleotide sequence of SEQ ID NO: 1; (ii) an assistant DNA sequence to promote integration into a host genome; and (iii) a polynucleotide sequence encoding a yeast selectable marker; (b) linearizing said integrating plasmid; and (c) transforming said linearized integrating plasmid into the yeast under conditions that permit recombination between the Candida utilis biotin synthase gene and the yeast genome.