Patent ID: 7809511

Claim:
A method for determining a metabolic flux that affects production of a desired substance, comprising the steps of: (A) creating a stoichiometric matrix based on formulas of biochemical reactions affecting production of said desired substance; (B) selecting a number of independent fluxes from all fluxes of said biochemical reactions, wherein the number of independent fluxes is the same as the degree of freedom of said stoichiometric matrix; (C) creating a number of flux distributions, wherein (i) each flux distribution is calculated from a random combination of said independent fluxes and (ii) said number is sufficient for a statistical analysis; and (D) obtaining a regression equation from said flux distributions, wherein said regression equation has a function that includes the minimum number of said independent fluxes for said function to show a correlation to the production of said desired substance, whereby a metabolic flux is determined that is comprised of said minimum number of independent fluxes and that affects the production of said desired substance, wherein said steps are performed by a computer that is specially programmed therefor.