Patent ID: 8637478

Claim:
A method of modulating expression of a target protein in a cell by altering splicing of a target pre-mRNA encoding the target protein comprising contacting the cell with an oligomeric compound comprising a contiguous sequence of nucleosides having the formula I: T 1 -(Nu 1 ) n1 -(Nu 2 ) n2 -(Nu 3 ) n3 -(Nu 4 ) n4 -(Nu 5 ) n5 -T 2 , wherein: Nu 1 and Nu 5 are, independently, 2′ stabilizing nucleosides; Nu 2 and Nu 4 are β-D-2′-deoxy-2′-fluororibofuranosyl nucleosides; Nu 3 is a 2′-modified nucleoside; each of n1 and n5 is, independently, from 0 to 3; the sum of n2 plus n4 is between 10 and 25; n3 is from 0 to 5; and each T 1 and T 2 is, independently, H, a hydroxyl protecting group, an optionally linked conjugate group or a capping group; wherein the oligomeric compound has a nucleobase sequence complementary to the target pre-mRNA; and thereby altering splicing of the pre-mRNA and thereby modulating expression of the target protein.