Patent ID: 7271002

Claim:
A method of producing an adeno-associated virus (AAV) in an insect cell, comprising: (i) providing at least one insect cell-compatible vector comprising a first nucleotide sequence comprising at least one AAV inverted terminal repeat (ITR) nucleotide sequence, a second nucleotide sequence comprising an open reading frame (ORF) comprising nucleotide sequences encoding AAV VP1, VP2, and VP3 capsid proteins operably linked to at least one expression control sequence for expression in an insect cell, a third nucleotide sequence comprising a Rep52 or a Rep40 coding sequence operably linked to at least one expression control sequence for expression in an insect cell, and a fourth nucleotide sequence comprising a Rep78 or a Rep68 coding sequence operably linked to at least one expression control sequence for expression in an insect cell, wherein said at least one vector is a baculoviral vector, a viral vector or a plasmid, (ii) introducing said at least one insect cell-compatible vector into an insect cell wherein the insect cell is Sf9, and (iii) maintaining said insect cell under conditions such that AAV is produced.