Patent ID: 8852606

Claim:
A method for conjugating a bacterial capsular polysaccharide to a carrier protein, comprising (a) purifying the polysaccharide, comprising the steps of (i) precipitation of the polysaccharide using one or more cationic detergents, followed by (ii) solubilisation of the precipitated polysaccharide using an alcohol, and (b) conjugating the polysaccharide to a carrier protein, wherein the bacterial capsular polysaccharide is from Neisseria meningitidis serogroup A, Neisseria meningitidis serogroup W135, Neisseria meningitidis serogroup Y, Haemophilus influenzae or Streptococcus pneumoniae , wherein the method further comprises a step of hydrolysis to form oligosaccharides prior to step (b).