Patent ID: 7135564

Claim:
A reusable linker arm for solid support oligonucleotide synthesis, the linker arm consisting of the following formula: Z-O-T [SUPPORT] wherein Z is selected from the group consisting of: HO 2 C—CH 2 —CH 2 —(C═O)—; HO 2 C—CH 2 —O—CH 2 —(C═O)—; HO 2 C—(C═O)—; and wherein: R 1 , R 2 and R 3 are the same or different and are selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C 1 –C 20 alkyl group, an substituted or unsubstituted C 5 –C 30 aryl group and a substituted or unsubstituted C 5 –C 40 alkylaryl group; R 4 and R 5 are the same or different and are selected from the group consisting of hydrogen, a substituted or unsubstituted C 1 –C 20 alkyl group, an substituted or unsubstituted C 5 –C 30 aryl group and a substituted or unsubstituted C 5 –C 40 alkylaryl group; X 1 is selected from the group consisting of —O—, —S—, —C(O)—, —S(O) 2 —, and —N(R)—; R is selected from the group consisting of hydrogen, a substituted or unsubstituted C 1 –C 20 alkyl group, an substituted or unsubstituted C 5 –C 30 aryl group and a substituted or unsubstituted C 5 –C 40 alkylaryl group; n is 0, 1 or 2; and one of A 1 and B 1 is selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C 1 –C 20 alkyl group, an substituted or unsubstituted C 5 –C 30 aryl group and a substituted or unsubstituted C 5 –C 40 alkylaryl group and the other of A 1 and B 1 has the formula: wherein p is 0 or 1; X 2 is selected from the group consisting of —O—, —S—, —C(O)—, —S(O) 2 —, and —N(R)—; R is selected from the group consisting of hydrogen, a substituted or unsubstituted C 1 –C 20 alkyl group, an substituted or unsubstituted C 5 –C 30 aryl group and a substituted or unsubstituted C 5 –C 40 alkylaryl group; R 6 and R 7 are the same or different and are selected from the group consisting of hydrogen, a substituted or unsubstituted C 1 –C 20 alkyl group, an substituted or unsubstituted C 5 –C 30 aryl group and a substituted or unsubstituted C 5 –C 40 alkylaryl group; and T has the formula: —[CH 2 ] q —[O—CH 2 —CH 2 —O] r —[CH 2 ] s — wherein q and s are the same or different and each is an integer having a value of 0–40 and r is an integer having a value of 1–200 or T has the formula: -[Q] a -CH 2 —CH(R a )—CH 2 —O—[CH 2 ] b — wherein a is 0 or 1, R a is selected from —OH, —NH 2 , —NHR and —OR wherein R is a protecting group and b is an integer having a value of 0–40, and Q is moiety having the formula: —[CH 2 ] u —[CH(R a′ )] t —[CH 2 ] q —O—[CH 2 ] r —O—[CH 2 ] s — wherein q, r, s, t and u are the same or different and each is an integer having a value of 0–40 and R a′ is selected from the group consisting of hydrogen, hydroxyl, a C 1 –C 40 alkyl group, a C 5 –C 40 aryl group, a C 1 –C 40 alkoxy group, a C 1 –C 40 ester group, a C 1 –C 40 hydroxy-containing group, a C 2 –C 40 acrylate-containing group, a C 5 –C 40 alkylaryl group, —NH 2 , —NHR and —OR, wherein R is a protecting group; and wherein the term SUPPORT is defined as an organic or an inorganic substance.