Patent ID: 8728798

Claim:
A method for producing adipic acid, comprising: (a) contacting a Candida yeast with a feedstock comprising a vegetable oil, wherein the Candida yeast comprises: (i) a disruption, deletion or knockout of (1) a polynucleotide that encodes a POX4 polypeptide or (2) a promoter operably linked to a polynucleotide that encodes a POX4 polypeptide, whereby POX4 activity is reduced or removed and POX5 activity is not reduced or removed, (ii) a disruption, deletion or knockout of (1) a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide or (2) a promoter operably linked to a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide, whereby a long chain acyl-CoA synthetase activity is reduced or removed, and (iii) a disruption, deletion or knockout of (1) a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide or (2) a promoter operably linked to a polynucleotide that encodes a long chain acyl-CoA synthetase polypeptide, whereby an acyl-CoA synthetase activity is reduced or removed; and (b) culturing the Candida yeast under conditions in which adipic acid is produced.