Patent ID: 7109023

Claim:
An immunochemical assay device comprising a base member and at least one array disposed on said base member, said array comprising: (i) a reservoir pad having sufficient porosity and volume to receive and contain a liquid; (ii) a wicking membrane disposed distally to said reservoir pad, said wicking membrane having sufficient porosity and volume to absorb and to wick a substantial proportion of The sample received in said reservoir pad; and (iii) at least one first filter zone which is separate and distinct from said reservoir pad and wicking membrane, and intemosed between and contiguous with said wicking membrane and said reservoir pad, said first filter zone having impregnated therein one or more labeled immunochemical components capable of binding to or competing with one or more analytes of interest in said sample to form one or more immuno-complexes, said one or more labeled immunochemical components comprises an antibody specific for amino acids 26–51 of NT-proBNP (SEQ ID No:1); (iv) at least one second filter zone which is separate and distinct from said reservoir pad, said wicking membrane and said first filter zone, and contiguous with said wicking membrane, and said first filter zone, said second filter zone having sufficient porosity and volume to receive and containing a liquid sample on which the assay is to be performed; at least one additional immobilized immunochemical component disposed in at least one assay indicia zone of said wicking membrane downstream of said reservoir pad and defining assay indicia, said at least one additional immobilized immunochernical component comprises an antibody specific for amino acids 1–25 of NT-proBNP (SEQ ID NO:1).