Patent ID: 8500990

Claim:
A method for determining information indicative of the level of an analyte in a liquid sample, the method comprising: reacting the liquid sample with a working electrode having a reagent matrix disposed thereon, the reagent matrix formed from a dispensing reagent containing an enzyme capable of catalyzing a reaction involving the analyte, a mediator that is considered an enzyme inhibitor, and an enzyme co-factor wherein the mediator is selected from the group consisting of meldola's blue, 4-methyl-1,2-benzoquinone, and 2,6-dichloroindophenol, the enzyme co-factor having a concentration in the dispensing reagent of about 0.01% to about 3.0% (w/w) and the mediator having a concentration in the dispensing reagent of about 0.05% to about 3.0% (w/w), wherein the working electrode provides a stable and sensitive response when the working electrode is stored at ambient conditions for a period of time of at least 3 months or longer; generating an electrical output from the reagent matrix; measuring the electrical output; and determining the level of the analyte in the liquid sample using information comprising the measured electrical output.