Patent ID: 7090991

Claim:
A method for identifying a compound that modulates a heterologous G protein-coupled receptor in a yeast cell, said method comprising: providing a first recombinant yeast cell which comprises a heterologous G protein-coupled receptor that is functionally integrated into a first pheromone response signal transduction pathway of said first yeast cell, wherein a signal molecule is produced by said first yeast cell upon activation of said first signal transduction pathway; providing a second recombinant yeast cell that does not comprise a heterologous G protein coupled receptor, but comprises a reporter gene, which produces a detectable signal upon activation of a second signal transduction pathway of said second yeast cell, in response to production of said signal molecule by said first yeast cell; contacting said first yeast cell with a test compound; contacting said second yeast cell with said signal molecule produced by said first yeast cell; and detecting said detectable signal produced by said second yeast cell, thereby identifying a compound that modulates a heterologous G protein-coupled receptor in a yeast cell.