Patent ID: 7919612

Claim:
A composition comprising a first oligomer and a second oligomer, wherein: the first and second oligomers each independently consist of 20 to 22 linked nucleosides and at least 20 contiguous nucleosides of the first oligomer are fully complementary to contiguous nucleosides of the second oligomer; the first oligomer is fully complementary to a target messenger RNA; the first oligomer comprises a first region consisting of two 2′-F nucleosides, a second region consisting of one 2′-OMe nucleoside, a third region consisting of two 2′-F nucleosides, a fourth region consisting of two 2′-OMe nucleosides, a fifth region consisting of five 2′-F nucleosides, a sixth region consisting of three 2′-OMe nucleosides, a seventh region consisting of three 2′-F nucleosides, and an eighth region consisting of two 2′-OMe nucleosides; the second oligomer comprises a plurality of nucleosides each comprising a 2′-OH, and the composition reduces the level of target messenger RNA present in a cell contacted with the composition.