Patent ID: 7186815

Claim:
An isolated DNA molecule comprising: (i) at least part of a transposon derivative including between its left terminus (LR) and its right terminus (RR) a sequence comprising a promoterless promoter reporter gene, a ribosome binding site and a secretion reporter gene without a sequence coding for a signal peptide (SP), the DNA molecule being without stop codons in the region upstream from the secretion reporter gene and (ii) a DNA sequence coding for a signal peptide selected from the group consisting of SP310 (SEQ ID NO:41), 310mut1 (SEQ ID NO:42), 310mut2 (SEQ ID NO:43), 310mut3 (SEQ ID NO:44), 310mut4 SEQ ID NO:45), 310mut5 (SEQ ID NO:46), 310mut6 (SEQ ID NO:47), 310mut7 (SEQ ID NO:48), 310mut8 (SEQ ID NO:49), 310mut10 (SEQ ID NO:51), 310mut11 (SEQ ID NO:52), 310mutA (SEQ ID NO:53), 310mutB (SEQ ID NO:54), 310mutC (SEQ ID NO:55), 310mutA1 (SEQ ID NO:56), 310mutB1 (SEQ ID NO:57), 310mutD2 (SEQ ID NO:58), 310mutD7(SEQ ID NO:59), 310mutE2 (SEQ ID NO:60), 310mutE11(SEQ ID NO:61) and 310mutF2 (SEQ ID NO:62).