Patent ID: 8431410

Claim:
A reagent kit that detects a reducible or oxidizable analyte by a redox reaction and a fluorimetric determination, the reagent kit comprising: (a) one or more enzymes that reduce or oxidize the reducible or oxidizable analyte; and (b) a redox indicator that is oxidized or reduced in the presence of both (i) the reducible or oxidizable analyte, and (ii) the one or more enzymes by accepting an electron, directly or via a mediator, from the reducible or oxidizable analyte, the redox indicator being a compound of formula (I): wherein R 1 and R 2 are each independently selected from R, (CH 2 CH 2 O) m R, COR, COOR, and OCOR; each R 3 is independently selected from NO 2 , CN, R, OR, OCOR, COOR, SR, and halogen; R is H or C 1 -C 4 alkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR 2 , COOR, CONR 2 , SO 3 R and salts thereof, and PO(OR) 3 and salts thereof; m is an integer from 1 to 20; and n is 1, 2, or 3 wherein the reducible or oxidzable analyte is glucose and the one or more enzymes is selected from the group consisting of glucose oxidase, glucose dye oxidoreductase, and glucose dehydrogenase/diaphorase.