Patent ID: 7588897

Claim:
A method for amplifying and detecting indicator enterococci nucleic acid in a sample, wherein said indicator enterococci nucleic acid is nucleic acid from E. faecalis, E. faecium, E. casseliflavus, E. gallinarum, E. mundtii, E. durans, E. hirae and/or E. columbae and not from E. avium, E. malodoratus, E. pseudoavium, E. raffinosus, E. saccharolyticus or E. dispar , comprising the steps of: a) contacting said sample with a first amplification oligomer comprising a target binding region consisting of SEQ ID NO: 30, a second amplification oligomer comprising a target binding region consisting of SEQ ID NO: 31, and a detection probe oligomer comprising a target binding region consisting of SEQ ID NO: 45; b) exposing said sample to conditions sufficient to amplify indicator enterococci nucleic acid if present in said sample with said first and second amplification oligomers to produce an amplified product; and c) determining whether said indicator enterococci nucleic acid is in said sample by using said detection probe oligomer to detect the presence or absence of the amplified product.