Patent ID: 8426150

Claim:
A process for increasing the concentration of at least one protein or peptide in a biological test sample, wherein the protein or peptide is differentially present in the biological test sample compared to a biological control sample, comprising: (a) extracting one or more soluble proteins or peptides from the biological control sample, and developing a composition of antigen binding immunoglobulins capable of binding at least one polypeptide in the biological control sample; (b) immobilizing the immunoglobulins onto a base matrix to prepare an affinity media; (c) extracting soluble proteins or peptides from a biological test sample; and (d) passing extracted soluble proteins from the biological test sample through a column packed with the affinity media, collecting the flow-through fraction and concentrating the proteins contained therein, wherein the differential proteins or peptides are enriched in the sample of interest.