Patent ID: 7858360

Claim:
A filamentous fungal cell in which an endogenous alkaline protease gene encoding a polypeptide having alkaline protease activity and having an amino acid sequence which has at least 85% identity to the amino acid sequence of SEQ ID NO:30, an endogenous neutral metalloprotease gene encoding a polypeptide having neutral metalloprotease activity and having an amino acid sequence which has at least 85% identity to the amino acid sequence of SEQ ID NO:31, an endogenous serine protease gene encoding a polypeptide having serine protease activity and having an amino acid sequence which has at least 85% identity to the amino acid sequence of SEQ ID NO:32, and an endogenous intracellular kexin maturase gene encoding a polypeptide having kexin maturase activity and having an amino acid sequence which has at least 85% identity to the amino acid sequence of SEQ ID NO:33 have been inactivated, whereby the cell expresses undetectable levels of alkaline protease, neutral metalloprotease, serine protease, and kexin maturase polypeptides or expresses functionally inactive alkaline protease, neutral metalloprotease, serine protease, and kexin maturase polypeptides.