Patent ID: 7771955

Claim:
A membrane-containing composition that directs the orientation of an immobilized peptide or protein on a hydrophobic membrane, said composition comprising: a) at least one said hydrophobic membrane, said at least one said hydrophobic membrane being selected from the group consisting of a thin porous substrate with straight pores, a polycarbonate membrane, and a track etched membrane; b) at least one tie layer layered on said hydrophobic membrane, said tie layer being selected from the group consisting of polyvinyl acetate and polyvinyl butyral; c) an amine functional layer layered on top of said at least one tie layer; said amine-functional layer being nickel-coordinated chitosan; d) a plurality of peptides or proteins comprising a histidine tag, wherein said histidine tag is attached to said nickel of said nickel-coordinated chitosan, and wherein said immobilized peptides or proteins are aligned on said amine functional layer, and wherein said peptides or proteins are capable of binding to antibodies or target biomolecules.