Patent ID: 8679857

Claim:
A method of extracting plasmid DNA from an unclarified lysate, comprising the steps of: a. providing an unclarified cell lysate; b. providing at least one pipette tip column, wherein the pipette tip column is comprised of i) a column body having an open upper end, an open lower end, and an open channel between the upper and lower ends of the column body, wherein the column body is comprised of a modified pipette tip; ii) a bottom frit extending across the open channel, said bottom frit having a thickness in the range of 20 μm to 1000 μm and a pore size in the range of 50 μm to 250 μm; and iii) a bed of extraction media positioned inside the open channel and in contact with the bottom frit; c. passing the unclarified cell lysate through the pipette tip column, wherein the plasmid DNA is captured by the bed of extraction media; d. passing wash solution through the pipette tip column, wherein the wash solution is comprised of an alcohol; e. passing a gas through the bed of extraction media, whereby the majority of bulk liquid is evacuated from the bed of extraction media; and f. eluting the plasmid DNA by passing a desorption solvent through the pipette tip column.