Patent ID: 8617862

Claim:
A non-naturally occurring microbial organism, comprising a microbial organism having a propylene pathway comprising at least one exogenous nucleic acid encoding a propylene pathway enzyme expressed in a sufficient amount to produce propylene; said non-naturally occurring microbial organism further comprising: (i) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein said at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a citryl-CoA synthetase, a citryl-CoA lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein said at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H 2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H 2 hydrogenase, and combinations thereof; wherein said propylene pathway comprises a pathway selected from: (a) a propylene forming enzyme and a 2-ketoglutarate methyltransferase; and (b) a propylene forming enzyme, a 4-hydroxy-4-methyl-2-ketoglutarate aldolase, a 4-hydroxy-4-methyl-2-ketoglutarate dehydratase II, a 4-hydroxy-4-methyl-2-ketoglutarate dehydratase I, a 4-methylene-2-ketoglutarate reductase, and a 4-methy-2-ketoglutaconate reductase.