Patent ID: 8030537

Claim:
A method for manufacturing a recombinant protein, comprising the steps of a) isolating fibroblast cells; b) transfecting the isolated fibroblast cells with a plasmid comprising a nucleotide sequence coding for the recombinant protein; c) isolating a nucleus from the transfected fibroblast cells; d) injecting the isolated nucleus into an enucleated bovine oocyte that is genetically identical to the isolated fibroblast cells; e) stimulating the oocyte to divide and form an embryo; f) introducing the embryo into a host bovine; g) allowing the embryo to develop into a fetus in the host bovine; h) harvesting the fetus from the host bovine; i) isolating cells from the fetus; j) introducing the isolated cells in a bovine animal; k) expressing the recombinant protein in a bovine animal; and l) obtaining the expressed recombinant protein from the bovine animal.