Patent ID: 7323336

Claim:
A method of generating a variant nucleic acid encoding a polypeptide having amylase activity comprising: (i) providing a template nucleic acid comprising (a) a polynucleotide sequence encoding a polypeptide having amylase activity or enzymatically active fragments thereof, wherein the polynucleotide sequence comprises at least about 95% sequence identity to SEQ ID NO:1, or (b) sequences complementary to (a); and (ii) modifying one or more nucleotides in said polynucleotide to another nucleotide, deleting one or more nucleotides in said polynucleotide, or adding one or more nucleotides to said polynucleotide, or any combination thereof, and selecting for a variant nucleic acid encoding a polypeptide having an amylase activity, thereby generating a variant nucleic acid encoding a polypeptide having amylase activity.