Patent ID: 7670806

Claim:
A method for catalyzing the release of ADP-ribose from poly(ADP-ribose) or O-acetyl-ADP-ribose, comprising contacting the poly(ADP-ribose) or O-acetyl-ADP-ribose with an isolated ADP-ribosyl acceptor hydrolase 3 (ARH3) polypeptide, wherein the ARH3 polypeptide comprises: a) the amino acid sequence set forth as one of SEQ ID NO: 2, 3, 4, or 6; b) an amino acid sequence at least 95% identical to the amino acid sequence set forth as SEQ ID NO: 2, wherein the polypeptide has poly(ADP-ribose) glycohydrolase activity or O-acetyl-ADP-ribose hydrolase activity; c) the amino acid sequence set forth as SEQ ID NO: 2, wherein a glutamic acid is substituted for a glutamine at positions 261 and 262; or d) the amino acid sequence set forth as SEQ ID NO: 2, wherein a glutamic acid is substituted for a glutamine at positions 238 and 239; thereby catalyzing the release of ADP ribose.