Patent ID: 8313923

Claim:
A process for enzymatically preparing a compound of formula (2) from an unsaturated alkene derivative of formula (I) wherein A is a nitro radical (—NO 2 ), a ketone radical (—CRO), an aldehyde radical (—CHO), or a carboxyl radical (—COOR), wherein R is H or an optionally substituted C 1 -C 6 -alkyl radical; R 1 , R 2 and R 3 are, independent of one another, H, C 1 -C 6 -alkyl, C 2 -C 6 -alkenyl, carboxyl, or a carbo- or heterocyclic, aromatic or nonaromatic radical, wherein said carbo- or heterocyclic, aromatic or nonaromatic radical is optionally substituted, or R 1 is linked to R 3 so as to become part of a 4-8-membered cycle, or R 1 is linked to R so as to become part of a 4-8-membered cycle, with the proviso that R 1 , R 2 , and R 3 may not be identical; comprising reducing a compound of formula (1) with a reductase comprising: (i) a polypeptide selected from the group consisting of SEQ ID NOs: 1, 2, and 3; or (ii) a functionally equivalent polypeptide having at least 95% sequence identity to a polypeptide selected from the group consisting of SEQ ID NOs: 1, 2, and 3.