Patent ID: 7223559

Claim:
A temperature-sensitive plasmid (i) which is obtainable from a plasmid carried by Escherichia coli DH5 α/pMTS11910 (FERM BP-6904) or Escherichia coli DH5 α/pMTS11914 (FERM BP-6905), (ii) which autonomously replicates in Escherichia coli K-12 at 10–30° C., (iii) at a temperature of 33° C. or more, is incapable of autonomous replication or is distributed unhomogeneously upon cell division of Escherichia coli K-12, and so is not stably carried within Escherichia coli K-12, and (iv) does not autonomously replicate or is distributed unhomogeneously in an Escherichia other than Escherichia coli K-12 at any temperature, and so is not stably carried within said Escherichia other than Escherichia coli K-12.