Patent ID: 7214769

Claim:
A method for preparing an immobilized peptide derivative of formula (I): Sub-[L]-[NH-A-C(O)] n+m —OH (I) comprising: (a) reacting an immobilized compound of formula (II): Sub-(L)-[NH-A-C(O)] n —OH (II) with an amino acid ester or peptide derivative of formula (III): H—[NH-A-C(O)] m —O(tBu) (III) in the presence of a coupling agent to yield an immobilized peptide derivative of general formula (IV): Sub-[L]-[NH-A-C(O)] n+m —O(tBu) (IV); (b) removing the t-Bu (t-butyl) group to produce an immobilized peptide derivative of general formula (I); wherein n is a positive integer, m is 1, Sub is a solid support, L is a linker to the amino acid ester or peptide derivative, each NH-A-C(O) independently is the residue of an optionally sidechain-protected amino acid; or the structure —NH-A represents a heterocyclic group.