Patent ID: 8906306

Claim:
A fluid transfer control method, the method comprising the steps of: providing a solution comprising a concentration of a control dye, transferring a pre-defined volume of the solution comprising the control dye to each of a plurality of vessels, measuring an intensity of the control dye within each vessel, comparing the intensity measured for each vessel with a pre-defined threshold value, whereby a fluid transfer error is detected for a vessel and the vessel is excluded from further analysis if the measured intensity is below the pre-defined threshold value, compiling a mean intensity value of all vessels having a measured intensity that is above the pre-defined threshold value, verifying for each vessel having a measured intensity that is above the pre-defined threshold value, if the intensity is within a pre-defined range surrounding the compiled mean intensity value, wherein a fluid transfer error is detected for a vessel and the vessel is excluded from further analysis if the measured intensity is not within the pre-defined range, and declaring a correct fluid transfer for each vessel having a measured intensity that is within the pre-defined range, transferring a pre-defined volume of a second solution comprising a concentration of a quencher molecule to each of the vessels declared as having a correct fluid transfer, wherein the quencher molecule quenches the intensity of the control dye, measuring a quenched intensity of the control dye within each vessel, calculating a quenching parameter value based on the measured quenched intensity for each vessel, wherein a fluid transfer error for a vessel is detected and the vessel is excluded from further analysis if a pre-defined quenching threshold criterion of the quenching parameter value is not fulfilled, compiling a mean quenching parameter value of all vessels having a calculated quenching parameter value that fulfills the pre-defined quenching threshold criterion, if the quenching parameter value is within a pre-defined second range surrounding the compiled mean quenching parameter value, whereby a fluid transfer error is detected for a vessel and a vessel is excluded from further analysis if the measured quenching parameter value is not within the pre-defined second range, and declaring a second correct fluid transfer for each vessel having a measured quenching parameter value that is within the pre-defined second range.