Patent ID: 8420372

Claim:
A method for the preparation of a recombinant porcine adeno-associated virus (AAV) particle comprising: providing a cell engineered to express: porcine AAV capsid protein VP1 consisting of the amino acid sequence as set forth in SEQ ID NO:24; porcine AAV capsid protein VP2 consisting of the amino acid sequence as set forth in SEQ ID NO: 25; porcine AAV capsid protein VP3 consisting of the amino acid sequence as set forth in SEQ ID NO:26; a minigene comprising two AAV inverted terminal repeats and a transgene inserted between the two inverted terminal repeats; and a functional rep gene compatible with said AAV inverted terminal repeats; sufficient helper functions to permit packaging of the minigene into a porcine AAV capsid particle; and recovering said recombinant particle from said cell.