Patent ID: 7390658

Claim:
A method for differentiating a lymphoid dendritic cell of CD8α + immunophenotype from human CD34 + mononuclear cells separated from peripheral blood, which comprises the steps of culturing 1×10 5 /ml of the human CD34 + mononuclear cells in two steps, first in a first medium consisting of 5% human serum albumin, 100 U/ml of penicillin, 100 ug/ml of streptomycin, 100 U/ml of L-glutamine and 50 ng/ml of GM-CSF for one week and then in a second medium consisting of 5% human serum albumin, 100 U/ml of penicillin, 100 ug/ml of streptomycin, 100 U/ml of L-glutamine and 200 U/ml of IFN-γ for one week.