Patent ID: 7306937

Claim:
A subtilisin variant of a precursor subtilisin wherein the subtilisin variant has the same net electrostatic charge at pH 6.0 as the net electrostatic charge of the precursor subtilisin at pH 6.0 and comprises the amino acid substitution R170S, and further comprises one or more amino acid substitutions selected from the group consisting of R45N, K251G and E271T, wherein the positions are numbered by correspondence with the amino acid sequence of Bacillus amyloliquefaciens subtilisin set forth in SEQ ID NO:3, the subtilisin variant has proteolytic activity, and is a mature subtilisin comprising the gaps present in the amino acid sequence of a Bacillus lentus subtilisin relative to the amino acid seguence of the Bacillus amyloliguefaciens subtilisin depicted in FIG. 3 .