Patent ID: 7763719

Claim:
A recombinant nucleic acid sequence encoding a humanized anti-TAG-72 antibody, wherein the humanized anti-TAG-72 antibody comprises: a light chain Complementarity Determining Region (L-CDR)1, a L-CDR2, and a L-CDR3; and a heavy chain Complementarity Determining Region (H-CDR)1, a H-CDR2, and a H-CDR3, wherein L-CDR3, H-CDR1, H-CDR2 and H-CDR3 are parental HuCC49 antibody Complementarity Determining Regions (CDRs), and wherein (i) L-CDR1 is from the human monoclonal LEN antibody and L-CDR2 is from the parental HuCC49 antibody, (ii) L-CDR1 is from the parental HuCC49 antibody and L-CDR2 is from the human monoclonal LEN antibody, or (iii) L-CDR1 and L-CDR2 are from the human monoclonal LEN antibody, wherein the human monoclonal LEN antibody L-CDR1 has the amino acid sequence set forth as SEQ ID NO: 7 and the human mononoclonal LEN antibody L-CDR2 has the amino acid sequence set forth as SEQ ID NO: 8, and wherein the humanized anti-TAG-72 antibody retains binding affinity for TAG-72 and has reduced immunogenicity, as compared to the parental HuCC49 antibody.