Patent ID: 7790182

Claim:
A method for inducing in a subject a protective immune response against poxvirus, comprising the steps of (a) administering to said subject an immunologically effective amount of a nucleic acid vaccine comprising the poxvirus nucleic acids of (1) a nucleic acid encoding L1R or its ortholog (2) a nucleic acid encoding A33R or its ortholog, (3) a nucleic acid encoding A27L or its ortholog, and (4) a nucleic acid encoding B5R or its ortholog, (b) subsequently administering to said subject an immunologically effective amount of a protein vaccine comprising the purified recombinant poxvirus proteins or peptides of (i) a protein or peptide encoded by the open reading frame of the monkeypox ortholog L1R gene or its ortholog, (ii) a protein or peptide encoded by the open reading frame of the monkeypox ortholog A27L gene or its ortholog, (iii) a protein or peptide encoded by the open reading frame of the monkeypox ortholog A33R gene or its ortholog, and (iv) a protein or peptide encoded by the open reading frame of the monkeypox ortholog B5R gene or its ortholog, and an adjuvant; and (c) subsequently administering to said subject a second immunologically effective amount of a protein vaccine comprising the purified recombinant poxvirus proteins or peptides of (i) a protein or peptide encoded by the open reading frame of the monkeypox ortholog L1R gene or its ortholog, (ii) a protein or peptide encoded by the open reading frame of the monkeypox ortholog A27L gene or its ortholog, (iii) a protein or peptide encoded by the open reading frame of the monkeypox ortholog A33R gene or its ortholog, and (iv) a protein or peptide encoded by the open reading frame of the monkeypox ortholog B5R gene or its ortholog, so as to induce a protective immune response against poxvirus in the subject.