Patent ID: 8614062

Claim:
A method of detecting whether seafood originates from a grouper species, comprising: collecting a sample of muscle from a fish; extracting RNA from the fish muscle; amplifying the RNA using nucleic acid sequence based amplification, wherein the nucleic acid sequence based amplification uses a forward primer and a reverse primer designed to specifically amplify a region of 16S rRNA of a grouper species; wherein the forward primer and the reverse primer are compatible with nucleic acid sequence based amplification; adding a molecular probe to the nucleic acid sequence based amplification reaction, wherein the molecular probe is SEQ ID NO: 7 and fluoresces when hybridized to a target molecule; hybridizing the molecular probe with the amplified RNA; and detecting that the seafood originates from a grouper species when the fluorescence from the molecular probe hybridized to the amplified RNA product is detected.