Patent ID: 7235370

Claim:
An assay method for determining an aminoglycoside comprising: combining a sample suspected of containing the aminoglycoside with an antibody specific for the aminoglycoside and with an aminoglycoside analog having the formula wherein A is CH 2 NH 2 , CHCH 3 NH 2 , or CHCH 3 NHCH 3 ; B is H or OH; D is H or OH; E is NH 2 or OH; G′ is NH 2 , NHCH 3 , NH—T, or NCH 3 —T; J is H or OH; L is H, CH 3 , or OH; Q is H or CH 2 OH; Y′ is H, C(═O)CH(OH)CH 2 CH 2 NH 2 , or T; T is a label; and T is present in only one of G′ or Y′; and wherein the aminoglycoside analog and the aminoglycoside in the sample compete to form a detectable complex with the antibody; and determining the presence or amount of the detectable complex as a measure of the aminoglycoside in the sample.