Patent ID: 8114651

Claim:
An isolated dioxygenase which has the following properties and is able to be isolated from Bacillus thuringiensis: (a) requires oxygen, Fe 2+ , ascorbic acid and 2-oxoglutaric acid, (b) has an optimum reaction pH of 5 to 8, (c) has an optimum reaction temperature of 45° C. or lower, (d) is inactivated at 50° C. or higher, (e) is inhibited by EDTA, Cu 2+ , and Zn 2+ , (f) comprises one or more subunits having a molecular weight of 29,000±2,000 as measured by sodium dodecylsulfate-polyacrylamide gel electrophoresis, (g) comprises the amino acid sequence of SEQ ID NO: 5 at the N-terminus.