Patent ID: 7422849

Claim:
A method for determining expression of a gene which encodes a human polypeptide that has PI3 kinase activity and a molecular weight of about 110 kiladaltons as determined by SDS-PAGE, comprising contacting a sample with a nucleic acid molecule which hybridizes specifically to a transcript of said gene wherein said transcript is RNA or cDNA, and is selected from the group consisting of (a) the nucleotide sequence set forth in SEQ ID NO: 32; (b) the nucleotide sequence set forth in SEQ ID NO: 35; and (c) the nucleotide sequence which hybridizes to the complement of at least one of (a) and (b), at IMNaCl, 10×Denhardt's solutions; 50 mM Tris-HCL (pH 7.4); 10 mM EDTA; 0.1% SDS; 100 μg/ml denatured herring sperm DNA at 65° C. for 16 hours, followed by a wash of 2×SSC; 0.1% SDS at 42° C., or a wash of 0.5×SSC/0.1% SDS at 50° C., or a wash at 0.1×SSC/0.1% SDS at 65° C., or a wash at 0.1×SSC/0.1% SDS, at 68° C. and determining said hybridization as a determination of expression of said gene.