Patent ID: 7429380

Claim:
A method for producing a human antibody, said method comprising: (a) introducing a first polynucleotide into a first mammalian myeloma cell, wherein the first polynucleotide comprises a first amplifiable marker and a sequence encoding a heavy chain polypeptide of a human antibody; (b) introducing a second polynucleotide into a second mammalian myeloma cell, wherein the second polynucleotide comprises a second amplifiable marker and a sequence encoding a light chain polypeptide of said human antibody; (c) culturing each of said first and second mammalian myeloma cells separately in the presence of an amplification agent, wherein the first and second amplifiable markers are the same; and (d) fusing the cultured cells produced by steps (a)-(c) to form a hybrid cell, wherein the hybrid cell expresses said human antibody; wherein the first cell expresses an irrelevant light chain and expresses the heavy chain prior to fusion with the second cell.