Patent ID: 8586707

Claim:
A method for preparation of a stapled peptide, comprising the steps of: a) providing a functionalized peptide, wherein the functionalized peptide comprises at least a first functionalized amino acid and a second functionalized amino acid, wherein the first functionalized amino acid comprises an alkene moiety and the second functionalized amino acid comprises a tetrazole moiety, wherein the second functionalized amino acid is the fourth or the seventh successive amino acid from the first functionalized amino acid, wherein the alkene and tetrazole moieties can react to form a pyrazoline moiety and the stapled peptide can exhibit helical secondary structure; and b) exposing the functionalized peptide to electromagnetic radiation of a wavelength from 300 to 320 nm such that a stapled peptide having a pyrazoline moiety, formed from reaction of the alkene moiety with the tetrazole moiety, and exhibiting helical secondary structure is formed.