Patent ID: 8247213

Claim:
A method for massive culture of Dinophysis acuminate , comprising; sub-culturing Myrionecta rubra at 10˜30° C. with continuous light, for which Myrionecta rubra and its prey Teleaulax are inoculated in sea water medium at the density ratio of 1:10-1:15; inoculating Myrionecta rubra cultured in the sub-culturing of Myrionecta rubra in the water bath supplied with the air purified by carbon cartridge filter after irradiation, and then mass-culturing Myrionecta rubra with stirring the cultured Myrionecta rubra in the sub-culturing of Myrionecta rubra and the sea water medium using air bubbles formed and supplied through air sparging nozzle equipped on the bottom part of the water bath at 10˜30° C. with continuous light; inoculating Dinophysis acuminate and Myrionecta rubra , the prey for Dinophysis acuminate , at the density ratio of 1:10-1:30, followed by sub-culturing of Dinophysis acuminate at 10˜30° C. with continuous light; and mass-culturing Dinophysis acuminate by inoculating the Dinophysis acuminate cultured in the sub-culturing of Dinophysis acuminate in the water bath of the mass-culturing of Myrionecta rubra.