Patent ID: 8859214

Claim:
A method of screening a plurality of chemical compounds not known to inhibit PAS kinase activity to identify a compound which inhibits PAS kinase activity, which comprises; a) contacting a test sample comprising PAS kinase activity with the plurality of compounds not known to inhibit PAS kinase activity; b) determining the level of phosphorylation of the PAS kinase in the test sample; wherein the level of PAS kinase phosphorylation correlates with the level of intracellular PAS kinase activity; c) comparing the intracellular activity of PAS kinase in the test sample with that in a control sample; and d) where inhibition of PAS kinase activity by the plurality of compounds is observed, separately determining the inhibition of PAS kinase activity of each compound included in the plurality of compounds, so as to thereby identify any individual compound included therein which inhibits PAS kinase.