Patent ID: 7144706

Claim:
A method of screening a test agent for the ability to increase or decrease the binding association of 220 kDa ankyrin-B with a 220 kDa binding target in an isolated cell and/or isolated cells, comprising: (i) contacting 220 kDa ankyrin-B, the membrane-binding domain of 220 kDa ankyrin-B, the spectrin-binding domain of 220 kDa ankyrin-B, or the death or C-terminal domain of 220 kDa ankyrin-B, with an inositol 1,4,5-triphosphate or ryanodine receptor binding target thereof in the presence and absence of said agent, and (ii) determining the effect of said test agent on the binding association of 220 kDa ankyrin-B, the membrane-binding domain of 220 kDa ankyrin-B, the spectrin-binding domain of 220 kDa ankyrin-B, or the death or C-terminal domain of 220 kDa ankyrin-B with said binding target wherein said test agent that increases or decreases said binding association is a candidate regulator of said binding association of the membrane-binding domain of 220 kDa ankyrin-B, the spectrin-binding domain of 220 kDa ankyrin-B, or the death or C-terminal domain of 220 kDa ankyrin-B.