Patent ID: 8715954

Claim:
A method of producing a serum-free C3A clonal cell line deposited as ATCC accession No. CRL-12461, wherein cells of the clonal cell line have a doubling time in serum-free medium of less than 70% of the doubling time in serum-free medium of a parental C3A cell line and maintain liver-specific biological activity, and wherein cells of the clonal cell line express alpha fetal protein, albumin, α-1-antichymotrypsin, α-1-antitrypsin, antithrombin III, complement C3, Factor V and transferrin, comprising: a) sequentially culturing cells of a parental C3A cell line deposited as ATCC accession No. SD 2078 or SD 3283, in a series of medium having incrementally decreasing concentration of serum, the final medium in the series being serum free, b) generating a clonal cell colony of the cells from the final medium in the series of a) in serum-free medium; and c) propagating the clonal cell colony in serum-free medium, thereby producing the serum-free C3A clonal cell line.