Patent ID: 7807868

Claim:
A method for mobilizing a viral replicon from a T-DNA, comprising: a) providing an Agrobacterium replicon having a T-DNA, wherein said T-DNA contains a viral replicon flanked by a first and a second target site for a first site-specific recombinase, wherein said first and said second target sites are identical and directly repeated with respect to one another; b) contacting a cell of a plant with an Agrobacterium carrying said Agrobacterium replicon under conditions that allow transfer of said T-DNA; c) providing a nucleotide sequence encoding said first site-specific recombinase or an active fragment or variant of said first site-specific recombinase by sexual breeding, wherein said nucleotide sequence is operably linked to a promoter that drives expression in said cell and wherein said fragment or variant of said first site-specific recombinase retains site-specific recombination activity.