Patent ID: 7405819

Claim:
A sample substrate adapted for use with fluorescence excitation light with a first wavelength and a second wavelength, comprising a base and a reflector, the reflector comprising a reflecting multilayer interference coating comprising a first layer system including multiple layers and an intermediate layer system including multiple layers, wherein the first layer system reflects the excitation light with the first wavelength and transmits the excitation light with the second wavelength, and thicknesses of the layers of the first layer system ensure that any fluorescent sample material deposited on top of said multilayer interference coating is located near an antinode of a standing wave formed by the excitation light with the first wavelength incident on said substrate, and wherein the intermediate layer system reflects the excitation light with the second wavelength, and a thickness of at least one layer of the intermediate layer system ensures that the sample material deposited on top of said multilayer interference coating is located near an antinode of a standing wave formed by the excitation light with the second wavelength incident on said substrate, and further wherein the reflecting multilayer interference coating causes the antinodes to be located at substantially the same position.