Patent ID: 7029434

Claim:
A method for treating glutaraldehyde fixed biological tissue to inhibit calcification of said tissue following implantation in a mammalian body, said method comprising the steps of; a) placing the glutaraldehyde fixed biological tissue in contact with an approximately 0.625 weight percent solution of glutaraldehyde consisting essentially of: a) glutaraldehyde; b) approximately 4.863 g/l HEPES buffer; c) approximately 2.65 g/l MgCl 2 ·6H 2 O; d) approximately 4.71 g/l NaCl; e) balance of solution double filtered H 2 O; and f) sufficient NaOH to adjust pH to approximately 7.4; and b) heating the glutaraldehyde to a temperature of between approximately 35–55° C.