Patent ID: 7985739

Claim:
A method of integrating an exogenous nucleic acid into a genome of at least one vertebrate cell, said method comprising: introducing into said vertebrate cell: (a) a Sleeping Beauty transposon comprising said exogenous nucleic acid flanked by inverted repeats that are recognized by a Sleeping Beauty transposase; and (b) a source of a mutant Sleeping Beauty transposase that provides for enhanced integration as compared to the Sleeping Beauty transposase of SEQ ID NO:01, wherein said mutant Sleeping Beauty transposase comprises one or more amino acid substitutions in amino acid residues selected from the group consisting of amino acid residues 13, 20, 29, 33, 34, 40, 49, 53, 55, 64, 69, 72, 83, 86, 88, 90, 91, 95, 96, 98, 103, 252, 254, 260, 270 and 277, in the amino acid sequence of SEQ ID NO:01; so that said exogenous nucleic acid is integrated into said genome.