Patent ID: 8093005

Claim:
A method of preparing a reactive solid support surface for surface sensitive detection, which method comprises the steps of: a) providing a solid support having a hydrogel coating with a plurality of binding elements, and activating said binding elements to a state capable of forming covalent bonds; b) coupling a protein resistant compound to the hydrogel via a first fraction of the activated binding elements; c) re-activating remaining unreacted binding elements on said hydrogel to a state capable of forming covalent bonds; and then d) coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, wherein the second fraction of the binding elements comprise those remaining on the hydrogel after coupling of the protein resistant compound via the first fraction of the binding elements; such that the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel via the first and second fractions of the binding elements, respectively and intermixed laterally thereon, and wherein said surface sensitive detection is a biosensor system based on mass sensing.