Patent ID: 8808551

Claim:
A method of separating a fraction from a whole sample, comprising: placing an obtained volume of a whole heterogeneous material, including at least a first liquid portion and a solid portion, obtained substantially simultaneously while reaming a bone, in a container having at least a first section and a second section; separating the solid portion from the first liquid portion at least by retaining at least a first portion of the solid portion of the obtained volume of a whole heterogeneous material in the first section of the container and allowing a separated portion of the first liquid portion to move to the second section; forming a fraction of at least the separated portion of the first liquid portion including at least undifferentiated cells in the second section of the container; and extracting the formed fraction from the second section of the container at least by allowing a first member to contact a second member; wherein forming the fraction of the liquid including at least undifferentiated cells in the second section of the container includes: positioning at least the container including the second section in a centrifuge device; and centrifuging the container in the centrifuge device so that a buoy including the first member and the second member moves within the second section; wherein extracting the formed fraction from the second section of the container includes accessing a volume between the first member of the buoy and the second member of the buoy through a passage in the first member of the buoy; wherein during extraction a first surface of the first member is configured to mate with a second surface of the second member.