Patent ID: 8795667

Claim:
A composition that comprises a dual affinity retargeting reagent, wherein said reagent immunospecifically binds to an epitope found on an MV form of the smallpox virus; wherein said dual affinity retargeting reagent comprises a first and a second polypeptide chain, being covalently linked to one another, wherein: (I) said first polypeptide chain comprises, from N-terminus to C-terminus: (A) a domain (I) comprising a binding region of a light chain variable domain of a first immunoglobulin (VL1); (B) a domain (II) comprising a binding region of a heavy chain variable domain of a second immunoglobulin (VH2); and (C) a domain (III) comprising CH2 and CH3 domains of an Fc region, and a hinge region; wherein domains (I) and (II) are covalently linked to one another such that they do not associate to form an epitope binding site; and (II) said second polypeptide chain comprises, from N-terminus to C-terminus: (A) a domain (IV) comprising a binding region of a light chain variable domain of the second immunoglobulin (VL2); and (B) a domain (V) comprising a binding region of a heavy chain variable domain of the first immunoglobulin (VH1); wherein domains (IV) and (V) are covalently linked to one another such that they do not associate to form an epitope binding site; wherein domains (I) and (V) associate to form an epitope binding site that immunospecifically binds said epitope found on said MV form of the smallpox virus, and domains (II) and (IV) associate to form an epitope binding site that immunospecifically binds a second epitope.