Patent ID: 8063018

Claim:
A molecule that binds thrombin and has the general formula of BM1-L-BM2, wherein: BM1 is a first thrombin binding moiety consisting of the amino acid sequence as set forth in SEQ ID NO: 4, SEQ ID NO: 19, SEQ ID NO: 35, SEQ ID NO: 69, SEQ ID NO: 70, SEQ ID NO: 71, SEQ ID NO: 72 or SEQ ID NO: 116, said first thrombin binding moiety having an affinity for a catalytic active site of thrombin; BM2 is a second thrombin binding moiety consisting of the amino acid sequence as set forth in SEQ ID NO: 20, said second thrombin binding moiety having an affinity for an exosite of thrombin that is not the catalytic active site; and L is a linker joining BM1 and BM2, said linker consisting of the amino acid sequence as set forth in SEQ ID NO: 17, SEQ ID NO: 48, SEQ ID NO: 49, SEQ ID NO: 109, SEQ ID NO: 110 or amino acids 9 to 186 of SEQ ID NO: 117, wherein said linker binds a calcium ion and undergoes a change in flexibility in response to the binding of the calcium ion, wherein BM1 and BM2 are selected such that each of the BM1 and BM2 existing separately has a lower binding affinity than the complex of BM1 and BM2 does when they are linked to form the molecule.