Patent ID: 8637327

Claim:
A method for optimizing the automatic fluorescence pattern recognition in immunodiagnosis, said method comprising the steps of: a) incubating a test field which includes a first amount of one or more indicator dyes formulated for the identification of selected structures of an organic material, a fluorescence dye for identification of at least one antibody, and the organic material, b) viewing the incubated test field with a camera to generate a first colour image having a first set of colour channels associated with the one or more indicator dyes and the fluorescence dye, each of said first colour channels having a corresponding colour intensity, wherein the one or more indicator dyes are visible in the first colour image in one or more of the first colour channels, c) differentially identifying in the first colour image the one or more indicator dyes and the fluorescence dye by the first colour channels, wherein the first colour channels include a detection colour channel associated with the fluorescence dye and thereby presenting a total detection colour intensity associated with the test field, and d) compensating for an interference effect of the one or more indicator dyes on the detection colour channel of the first colour image, including the steps of: e) incubating a calibration field comprising a second amount of the one or more indicator dyes without fluorescence dye, f) determining the amount of the interference of the one or more indicator dyes on the detection colour channel of the first colour image by viewing the incubated calibration field to generate a second colour image having a second set of colour channels, wherein the second colour channels include an indicator colour channel corresponding to the detection colour channel of the first colour image and having an indicator colour intensity that is the amount of interference of the one or more indicator dyes on the detection colour channel of the first colour image, and g) prior to the automatic fluorescence pattern recognition, correcting the detection colour channel presenting the total detection color intensity of the first colour image by the amount of the measured interference of the indicator dye by subtracting the indicator colour intensity obtained in the calibration field from the total detection colour intensity.