Patent ID: 7202212

Claim:
A method for decreasing the activity of NFκB transcription factor within a targeted collection of viable cells in-situ, said method comprising the steps of: identifying a collection of cells comprising viable cells in-situ as a target for decreased NFκB activity; providing means for effecting an introduction of at least one member selected from the group consisting of the PR-39 oligopeptide collective to the cytoplasm of said targeted collection of cells; introducing at least one member of the PR-39 oligopeptide collective to the cytoplasm of said targeted collection of cells using said effecting means; allowing said introduced PR-39 oligopeptide collective member to interact with such IκBα and proteasomes as are present within the cytoplasm of said cells in said targeted collection whereby (a) at least some of the proteasomes interact with said introduced PR-39 oligopeptide collective member, (b) at least a part of the proteolytic activity mediated by said proteasomes becomes selectively altered by said interaction with said introduced PR-39 oligopeptide collective member, (c) the selectively altered proteolytic activity of said proteasomes results in a marked inhibition of IκBα degradation in-situ within the cytoplasm of said cells in said targeted collection, and (d) said reduction of IκBα degradation in-situ results in a decrease in activity for such NFκB transcription factor as is present in-situ within the cytoplasm of said cells in said targeted collection.