Patent ID: 7892778

Claim:
A method for detecting the activity of γ-secretase, the method comprising: A. providing a transgene encoding a fusion protein, said transgene comprising: a) a first nucleotide sequence encoding the amino acid sequence LDA EFRHDSGYEV HHQKLVFFAE DVGSNKGAII GLMVGGVVIA TVIVITLVML KK (SEQ ID NO. 6); b) a second nucleotide sequence, at the 5′ end of the first nucleotide sequence, encoding a signal peptide; and c) a promoter, and a nucleotide sequence at the 3′ end of the first nucleotide sequence coding for a protein consisting of a DNA-binding domain and a transcription-activating domain to be expressed as a fusion protein with SEQ ID NO. 6; B. expressing the fusion protein in an isolated cell or transgenic C. elegans that contains γ-secretase said isolated cell or C. elegans having a reporter system activatable by release of a polypeptide that comprises VIVITLVML (SEQ ID NO. 3) said released polypeptide that comprises SEQ ID NO. 3 not comprising SEQ ID NO. 6; and C. detecting said polypeptide that comprises VIVITLVML (SEQ ID NO. 3) by observing a report of said reporter system.