Patent ID: 7405281

Claim:
A composition, comprising a labeled nucleotide analog, comprising the formula: wherein B is a natural or non-natural nucleobase; S is selected from a sugar moiety, an acyclic moiety and a carbocyclic moiety; L is a detectable label; R 1 is selected from O and S; R 2 , R 3 and R 4 are independently selected from O, NH, S, methylene, substituted methylene, C(O), C(CH 2 ), CH 2 CH 2 , C(OH)CH 2 R where R is 4-pyridine or 1-imidazole, provided that R 4 is additionally selected from R 5 , R 6 , R 7 , R 8 , R 11 and R 13 are independently selected from O, BH 3 , and S; and R 9 , R 10 and R 12 are independently selected from O, NH, S, methylene, substituted methylene, C(O), C(CH 2 ), CH 2 CH 2 , C(OH)CH 2 R where R is 4-pyridine or 1-imidazole; provided that at least one of R 2 , R 3 and R 4 is not O, and provided further that if R 2 and R 3 are O, and R 4 is, selected from than at least one of R 9 , and R 10 , is not O.