Patent ID: 8535876

Claim:
A laser microdissection method comprising: mounting a biological specimen on a planar carrier; cutting a dissectate as a desired part of the specimen along a closed cutting line from the biological specimen by laser pulses of a focused laser beam, wherein the laser pulses and the cutting line are determined by parameters; and causing a series of cutting holes by relative movement of the focused laser beam to the specimen such that the laser beam describes the closed cutting line on the specimen in order to separate said dissectate from the rest of the surrounding biological specimen, wherein at least one of the parameters that determine the laser pulses and the cutting line is continuously varied synchronously with the laser pulses along the closed cutting line, and wherein the at least one of said parameters being varied comprises at least one of an aperture, an attenuation, a density of the individual laser points on the cutting line and a focal position of the laser pulses.