Patent ID: 7285265

Claim:
A recombinant subgroup B adenovirus particle comprising an adenovirus genome wherein the genome comprises a deletion in the E1-region, which adenovirus genome does not encode a functional E1B 55K gene product, said adenovirus genome comprising a functional pIX coding sequence under control of an expression sequence, said expression sequence-comprising part of the 3′ end of the E1 B 55K coding sequence-containing from 150 to 600 nucleotides of adenovirus sequences directly upstream of the pIX open reading frame of a wild-type subgroup B adenovirus, and said expression sequence being capable of increasing expression of the pIX coding sequence in a packaging cell, relative to the expression of said pIX coding sequence when preceded by sequences between the E1B 55K stop codon and the start codon of pIX and without nucleotides from the 3′ end of the E1B55K coding sequence.