Patent ID: 8709492

Claim:
A method of preparing a plasma-protein-containing medicament from citrated plasma or from a citrate-containing plasma fraction, wherein the medicament has a reduced concentration of undesired metals selected from the group consisting of aluminum, cadmium, zinc, lead and iron, wherein the method comprises the steps of: (a) obtaining citrated plasma or citrate-containing plasma fraction by Cohn fractionation; (b) adding to the citrated plasma or citrate-containing plasma fraction, a compound to achieve a solution having the compound concentration of between 0.001 and 1.00 mol/liter, wherein the compound is selected from the group consisting of a monocarboxylate, a dicarboxylate, a monocarboxylic acid and a dicarboxylic acid; (c) exchanging the citrate and citrate-bound metals that may be present in the plasma or plasma fraction with the compound by diafiltration, ultrafiltration or chromatography at a temperature above 0° C. to 50° C. and at a pH of 6 to 8, under non-precipitating conditions, thereby reducing the concentration of citrate and the undesired metals that may have been present; (d) recovering one or more plasma proteins; and (e) finishing the medicament.