Patent ID: 7449149

Claim:
A kit useful for sequencing a nucleic acid, comprising a set of four different terminating nucleotides, each of which terminates a template-dependent primer extension reaction at a different template nucleotide and comprises a fluorescent label capable of producing a detectable, spectrally resolvable fluorescent signal, wherein at least one of the fluorescent labels is an energy transfer dye comprising: a xanthene donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and a non-nucleosidic linker linking the xanthene donor dye to the 4,7-dichlororhodamine acceptor dye.