Patent ID: 8071284

Claim:
A method for determining susceptibility of a human immunodeficiency virus 1 (HIV-1) to a non-nucleoside reverse transcriptase inhibitor (NNRTI) or a nucleoside reverse transcriptase inhibitor (NRTI), comprising detecting whether a mutation at codon 348 or 369 is present in a pol gene encoding reverse transcriptase of the HIV-1, wherein the codon number of said reverse transcriptase corresponds to the codon number in the wild type HIV-1 isolate NL4-3 sequence; wherein the mutation at codon 348 encodes isoleucine (I) instead of asparagine (N); wherein the mutation at codon 369 encodes isoleucine (I) or alanine (A) instead of threonine (T); and wherein the presence of the mutation correlates with reduced susceptibility to an NNRTI or to an NRTI, such that if the mutation is present, the HIV-1 has reduced susceptibility to the NNRTI or to the NRTI.