Patent ID: 8465781

Claim:
A method for preparation and purification of an enriched plant extract from Solanum glaucophyllum having an enriched content of 1,25-dihydroxyvitamin D 3 glycosides and quercetin glycosides, wherein the method comprises the following steps: (a) extracting plants or parts thereof from the species Solanum Glaucophyllum using an alcohol/water mixture having an alcohol/water ratio (%) of 75/25 to 25/75 (v/v), the alcohol selected from the group consisting of methanol, ethanol and isopropanol; and (b) purifying the plant extract obtained according to step a), using the steps: b1) applying the plant extract obtained according to step a) to a column comprising a non-ionic polymer resin selected from the group consisting of polystyrene, styrene-divinyl-benzene copolymers, acrylic ester polymers and polyphenolic resins; b2) washing the column with water; b3) eluting the enriched plant extract from the column; and b4) concentrating and/or drying the enriched plant extract.