Patent ID: 8877208

Claim:
A method of inducing an immune response in a subject, comprising: a) providing a multivalent immunogenic composition comprising: i) a nanoemulsion comprising: 1) 30-90%, 60-80%, or 60-70% by volume oil; 2) 3-15% by volume solvent, wherein said solvent is selected from the group consisting of ethanol and glycerol; 3) 3-15% by volume surfactant, wherein said surfactant is selected from the group consisting of polyoxyethylenesorbitan monolaurate and polyoxyethylenesorbitan monooleate; 4) cetylpyridinium chloride (CPC); and 5) water; and ii) a plurality of different immunogens consisting of Bacillus anthracis immunogens, Clostridium botulinum immunogens, and Yersinia pestis immunogens, wherein said Yersinia pestis immunogens are LcrV or LcrV10; and b) administering said multivalent immunogenic composition to said subject under conditions such that said multivalent immunogenic composition concurrently induces a Bacillus anthracis -specific immune response, a Clostridium botulinum -specific immune response, and a Yersinia pestis -specific immune response in said subject.