Patent ID: 8865176

Claim:
A dock and lock (DNL) complex comprising: a) a first fusion protein comprising (i) an anchoring domain (AD) from an A-kinase anchoring protein (AKAP), wherein the amino acid sequence of the AD moiety is selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:5; and (ii) a first effector protein selected from the group consisting of a first antibody and a first antigen-binding antibody fragment; and b) a second fusion protein comprising (iii) a dimerization and docking domain (DDD) moiety, wherein the amino acid sequence of the DDD moiety is selected from the group consisting of residues 1-44 of human protein kinase A (PKA) RIIα, residues 1-44 of human PKA RIIβ and residues 12-61 of human PKA RIα; and (iv) a second effector protein selected from the group consisting of a second antibody and a second antigen-binding antibody fragment; wherein two copies of the DDD moiety form a dimer that binds to the AD moiety to form the DNL complex.