Patent ID: 8524446

Claim:
A method for detecting adeno-associated virus (AAV) nucleotide sequences from an AAV signature region within an AAV capsid gene in a sample from a human or non-human primate, comprising performing a plurality of polymerase chain reactions (PCR) on the sample under conditions that allow for specific amplification of the AAV signature region with a pair of PCR primers consisting of a first PCR primer and a second PCR primer that are designed to amplify an AAV signature region located within the AAV capsid gene in the position of nucleotides 2886 to 3143 of SEQ ID NO:6, wherein the first PCR primer and the second PCR primer form a duplex with AAV sequences which flank said AAV signature region; wherein the presence of amplicons resulting from the PCR reactions indicates that AAV nucleotide sequences of the AAV signature region have been detected in the sample.