Patent ID: 6872564

Claim:
A transformant comprising a host cell, wherein the polyhydroxybutanoic acid polymerase gene in the host cell is disrupted with a recombinant vector containing a polyester polymerase gene, a β-ketothiolase gene, and a NADPH-acetoacetyl CoA reductase gene, wherein the polyester polymerase gene comprises a DNA encoding a protein selected from the group consisting of: (a) a protein having an amino acid sequence represented by SEQ ID NO:2 or 4, and (b) a protein having an amino acid sequence including deletion, substitution, or addition of between 2 and 10 amino acids relative to the amino acid sequence represented by SEQ ID NO:2 or 4, and having polyester polymerase activity, wherein the β-ketothiolase gene comprises a DNA encoding a protein selected from the group consisting of: (a) a protein having an amino acid sequence represented by SEQ ID NO:6, and (b) a protein having an amino acid sequence including deletion, substitution, or addition of between 2 and 10 amino acids relative to the amino acid sequence represented by SEQ ID NO:6, and having β-ketothiolase activity, and wherein the NADPH-acetoacetyl CoA reductase gene comprises a DNA encoding a protein selected from the group consisting of: (a) a protein having an amino acid sequence represented by SEQ ID NO:8, and (b) a protein having an amino acid sequence including deletion, substitution, or addition of between 2 and 10 amino acids relative to the amino acid sequence represented by SEQ ID NO:8, and having NADPH-acetoacetyl CoA reductase activity.