Patent ID: 7923256

Claim:
A method of determining a coagulation parameter in a sample using a single-use cartridge in combination with an analyzer, wherein the cartridge includes an entry port for receiving a sample, an entry port closure, a holding chamber in communication at a first end with the entry port, and a capillary stop in communication with the holding chamber at a second end, wherein the capillary stop is in communication with an analysis chamber containing a reagent, a conductimetric sensor sensitive to the displacement of a fluid sample across the conductimetric sensor and an amperometric sensor, wherein the holding chamber is in communication with an overflow chamber for receiving and retaining excess sample, wherein the overflow chamber is in communication with a pneumatic pump actuated by the analyzer, wherein the analyzer is capable of actuating the pneumatic pump to displace the sample from the holding chamber, the method comprising the steps of: (a) applying the sample to the entry port; (b) filling the holding chamber; (c) closing the entry port; (d) permitting excess sample to enter and be retained in the overflow chamber;(e) inserting the cartridge into the analyzer; (f) actuating the pneumatic pump to deliver a metered portion of the sample into the analysis chamber; (g) reacting the sample with the reagent in the analysis chamber; (h) positioning the sample in the analysis chamber with respect to the conductimetric sensor; (i) detecting a reaction product from the reagent at the amperometric sensor; and (j) determining the coagulation parameter from an output of the amperometric sensor.