Patent ID: 7504363

Claim:
A method for storage and analysis of a nucleic acid containing moiety in a biological sample, the method comprising: a. providing a device comprising a plurality of layers, wherein the plurality of layers comprises: i. a collection layer comprising a neutral porous matrix and a dry anticoagulant or liquefying agent; ii. a filter layer comprising a dry solid medium comprising a solid matrix having a particle size limit that precludes eukaryotic cells, but not prokaryotic cells or virions; iii. a reagent free separator layer; and iv. an isolation layer comprising a dry solid medium comprising a neutral solid matrix having sorbed thereto a composition comprising a detergent; b. applying a biological sample to the collection layer; c. filtering remaining components of the biological sample through the collection layer to the filter layer; d. filtering remaining components of the biological sample through the filter layer to the separator layer; e. filtering remaining components of the biological sample through the separator layer to the isolation layer; f. retaining nucleic acid components in the isolation layer while removing a plurality of non-nucleic acid components; g. drying the isolation layer; h. providing at least one primer; and i. analyzing the nucleic acid components using PCR techniques, wherein at least two PCR primers are used.