Patent ID: 8580571

Claim:
A method for producing a biosensor comprising a substrate to which a hydrophilic polymer is being bound, the method comprising the steps of: (a) forming a self-assembled monolayer on a substrate, wherein the self-assembled monolayer is formed by an alkanethiol; (b) coating a solution containing a photo radical generator onto the substrate obtained in step (a) to allow the photo radical generator to bind to the self-assembled monolayer on the substrate, and then drying said substrate, (c) then coating a solution containing a hydrophilic polymer onto the substrate obtained in step (b), and then drying said substrate, wherein the hydrophilic polymer is a polysaccharide having a carboxyl group and a double bond and (d) exposing the substrate obtained in step (c) to a light to generate a reactive group from the photo radical generator and to covalently bind the hydrophilic polymer to said reactive group via the double bond of the hydrophilic polymer, whereby the biosensor comprising a substrate to which a hydrophilic polymer is being bound is produced, wherein the carboxyl group contained in the hydrophilic polymer bound to the substrate in the biosensor is used for immobilizing a physiologically active substance of interest onto the biosensor.