Patent ID: 8609335

Claim:
A method of determining sequence information for a target polynucleotide, comprising: (a) providing a plurality of concatemers, each concatemer having been obtained by: (i) forming a first circular DNA comprising a fragment of said target polynucleotide and a first adaptor, wherein the first adaptor comprises a binding site for a restriction enzyme that cleaves DNA at a cleavage site separated from said binding site by at least six nucleotides; (ii) forming a linearized DNA by a process that comprises cleaving the first circular DNA at a site that is internal to the target polynucleotide using a restriction endonuclease that binds to said binding site, whereby the linearized DNA comprises the first adaptor flanked on each side by a portion of said fragment; (iii) forming a second circular DNA comprising said linearized DNA and a second adaptor; (iv) amplifying the second circular DNA to form said concatemer; (b) forming an array of said concatemers bound to a surface; and (c) determining target sequences both upstream and downstream from the second adaptor by a process that comprises hybridizing oligonucleotides to the second adaptor in a plurality of the concatemers in the array.