Patent ID: 8879072

Claim:
A method for operating a laser scanning microscope, the method comprising: carrying out a two-dimensional horizontal image scanning in a plurality of focus positions or height positions; detecting, accordingly in a channel of each of at least two detectors, the sample light; recording three-dimensional image stacks simultaneously with the at least two detectors; splitting the sample light unequally between the at least two detectors with a color neutral beamsplitter; and calculating, for individual picture points and an associated height position, a measured intensity value of each of the two detector channels by picture point; wherein, when each of the two intensity values lies within a threshold region of the respective detector channel, a sample image is generated from the two calculated intensity values in that an average is formed between the two intensity values; wherein, when only one of the two intensity values lies within a threshold region of the respective detector channel, a sample image is generated from only one of the two intensity values; and wherein, when neither of the two intensity values lies within a threshold region of the respective detector channel, a sample image is generated using neither of the two intensity values.