Patent ID: 7833406

Claim:
A gene detection method for detecting a gene having a specific sequence, said method comprising: a gene sample conformation step of conforming a gene sample by denaturing a gene to be detected into a single strand; an immobilization step of immobilizing, onto an electrode, a single-stranded nucleic acid probe having a base sequence that is complementary to a base sequence of the gene sample to be detected; a double-stranded nucleic acid formation step of adding the gene sample to the electrode on which the nucleic acid probe is immobilized, thereby forming a double-stranded nucleic acid in which the nucleic acid probe and the gene sample are hybridized; an intercalator addition step of adding, to the electrode on which the double-stranded nucleic acid is formed, an intercalator that has a substitution group comprising one of compounds represented by -Lb-Ib and -Lc-Fb or a combination of the both compounds, at a site shown in a general formula (1): Fa-La-Ia (1) wherein Fa and Fb are electrochemical active sites having electrochemical activity, Ia and Ib are double-stranded nucleic acid binding sites which are specifically intercalated into the double-stranded nucleic acid, and are capable of covalently binding double-stranded nucleic acid by light irradiation, and La, Lb, and Lc are connecting sites which connect the double-stranded nucleic acid binding sites with the electrochemical active sites; a light irradiation step of covalently bonding the double-stranded nucleic acid and the intercalator by performing light irradiation; and a detection step of detecting the intercalator that is covalently bonded to the double-stranded nucleic acid, by electrochemical measurement thereby detecting a gene having a specific sequence in a specimen.