Patent ID: 7951561

Claim:
A method for the preparation of κ-carrageenase, said method comprising the steps of: (i) growing a halotolerant marine bacterium, Pseudomonas sp., accession number MTCC 5261, in a liquid medium comprising carrageenan, yeast extract, sodium chloride, K 2 HPO 4 and KH 2 PO 4 in a concentration ratio ranging from 0.1:1.0:50:1:0.5 to 3.0:1.0:15.0:0.5:0.05, respectively, for 16-72 hours at 15-500° C. to obtain a culture; (ii) centrifuging the culture obtained from step (i) at 5000-8000 rpm for 20-40 min. to obtain a cell free extract as a supernatant; (iii) treating the supernatant obtained from step (ii) with ammonium sulphate at a temperature in the range of 3-15° C. and aging the mixture for a period in the range of 12-36 hours followed by centrifugation at 5000-8000 rpm at 3-15° C. to obtain a pellet containing κ-carrageenase; (iv) suspending the pellet obtained from step (iii) in Tris-HCl buffer solution to obtain a suspension containing κ-carrageenase; and (v) dialyzing the suspension obtained in step (iv) for removing adhered ammonium sulphate followed by gel filtration to obtain purified κ-carrageenase.