Patent ID: 7414111

Claim:
A library of IgA antibodies prepared in accordance with a method, said method comprising: a) providing a diverse population of templates that encode at least a portion of an IgA antibody; b) contacting the diverse population of templates with at least one primer, the at least one primer having a first portion which anneals to the templates and a second portion of predetermined sequence which does not anneal to the templates; c) synthesizing polynucleotides that are complementary to the portion of the templates between the location at which the first portion of the primer anneals to the template and the end of the templates, each of the polynucleotides having a primer at a first end thereof and a second end; d) separating the polynucleotides synthesized in step (c) from the templates; e) annealing at least one template oligonucleotide to the second end of the polynucleotides synthesized in step (c), the at least one template oligonucicotide having a first portion that anneals to the second end of the polynucleotides and a second portion having the same predetermined sequence as the second portion of the primer; f) extending the polynucleotides synthesized in step (c) to provide a terminal portion thereof that is complementary to the predetermined sequence; and g) amplifying the extended polynucleotides using only a single primer, wherein the single primer has the predetermined sequence.