Patent ID: 8779241

Claim:
A method of making a Cucumber Vein Yellowing Virus (CVYV) and Cucumber Green Mottle Mosaic Virus (CGMMV) resistant cucumber plant ( Cucumis sativus L.) comprising: a) providing a first cucumber plant ( Cucumis sativus L.), wherein said first cucumber plant is a cucumber plant ( Cucumis sativus L.) deposited at NCIBM under accession number 41635, said first cucumber plant comprising a Cucumber Vein Yellowing Virus (CVYV) and Cucumber Green Mottle Mosaic Virus (CGMMV) resistance conferring genetic element; b) transferring the identified Cucumber Vein Yellowing Virus (CVYV) and Cucumber Green Mottle Mosaic Virus (CGMMV) resistance conferring genetic element into a second cucumber plant ( Cucumis sativus L.) thereby conferring Cucumber Vein Yellowing Virus (CVYV) and Cucumber Green Mottle Mosaic Virus (CGMMV) resistance to said second cucumber plant ( Cucumis sativus L.), wherein said transferring comprises detecting a nucleic acid amplification fragment of 245 to 247 base pairs using molecular amplified fragment length polymorphism (AFLP) primers SEQ ID NO: 3 and SEQ ID NO: 4 in a molecular amplified fragment length polymorphism (AFLP) assay using the genome of said second cucumber plant ( Cucumis sativus L.) as template.