Patent ID: 8133718

Claim:
An analytical strip, comprising a substrate having at least one channel thereon, wherein the channel includes a first region, a second region and a third region arranged successively, and the first region being used for a fluid sample to be introduced thereinto, the analytical strip being characterized by: a first antibody localized in the first region for recognizing an analyte in the fluid sample; a saccharide localized in the first or the second region; a peroxidase localized in the first or the second region; a second antibody immobilized in the second region for recognizing the analyte in the fluid sample, wherein the second antibody and the first antibody are configured to recognize different epitopes of the analyte in the fluid sample; and a substrate reagent localized in the third region and comprising a saccharide oxidase; wherein upon introduction of the fluid sample containing the analyte into the channel, the first antibody, the saccharide and the peroxidase flow along with the fluid sample, and then a part of the peroxidase binds with the first antibody, the analyte and the second antibody so as to be retained in the second region, allowing the other part of the peroxidase to flow along with the fluid sample to the third region, the saccharide arrives at the third region and is catalyzed by the saccharide oxidase so as to produce hydrogen peroxide, and then the hydrogen peroxide reacts with the peroxidase arriving at the third region so as to generate an electrical signal.