Patent ID: 7022488

Claim:
A method for identifying a compound that modulates the activity of a T1R or T2R taste receptor by a Gi protein signaling pathway comprising: i. providing a eukaryotic cell that expresses at least one functional T1R or T2R taste receptor and a Gi protein that couples thereto; ii. contacting said eukaryotic cell with at least one compound that putatively modulates the activity of said T1R or T2R; iii. assaying the effect of said putative modulatory compound on at least one of of MAPK activation, cAMP accumulation or adenylyl cyclase activity in said eukaryotic cell; iv. identifying whether said at least one putative modulatory compound modulates the activity of such functional T1R or T2R expressed by said eukaryotic cell by a G i protein signaling pathway based on its effect on at least one of MAPK activity, cAMP accumulation or adenylyl cyclase activity.