Patent ID: 7347943

Claim:
A method for isolating a protein, comprising, a) suspending chromatography particles in at least one chamber in a centrifugal force field wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles, and forming a chromatography bed, wherein each particle is independently suspended without the need for support structure in relation to every other particle to allow for efficient exposure to all surfaces of the particle and the density and shape of the bed may be changed by changing the force parameter on the particles; b) adding a heterogeneous liquid comprising a protein, c) separating one or more portions of the heterogeneous liquid by the actions of the chromatography bed; and d) retaining a separated portion comprising the protein.