Patent ID: 8637294

Claim:
A nucleic acid encoding a Hypocrea jecorina CBH I variant consisting essentially of an amino acid sequence having at least 96.4% sequence identity to the amino acid sequence of SEQ ID NO:2, and comprising one or more alterations as compared to SEQ ID NO:2, wherein said one or more alterations is selected from the group consisting of: i. A112E/T226A; ii. S196T/S411F; iii. E295K/S398T; iv. T246C/Y371C; v. V403D/T462I; vi. T41I plus deletion at T445; vii. A68T/G440R/P491L; viii. G22D/S278P/T296P; ix. T246A/R251A/Y252A; x. T380G/Y381D/R394A; xi. Y252Q/D259W/S342Y; xii. S113T/T255P/K286M; xiii. P227L/E325K/Q487L; xiv. P227T/T484S/F352L; xv. Q17L/E193V/M213I/F352L; xvi. S8P/N49S/A68T/S113N; xvii. S8P/N49S/A68T/S113N/P227L; xviii. T41I/A112E/P227L/S278P/T296P; xix. S8P/N49S/A68T/A112E/T226A; xx. S8P/N49S/A68T/A112E/P227L; xxi. S8P/T41I/N49S/A68T/A112E/P227L; xxii. G22D/N49S/A68T/P227L/S278P/T296P; xxiii. S8P/G22D/T41I/N49S/A68T/N103I/S113N/P227L/S278P/T296P; xxiv. G22D/N49S/A68T/N103I/S113N/P227L/S278P/T296P; xxv. G22D/N49S/A68T/N103I/A112E/P227L/S278P/T296P; xxvi. G22D/N49S/N64D/A68T/N103I/S113N/S278P/T296P; xxvii. S8P/G22D/T41I/N49S/A68T/N103I/S113N/P227L/D249K/S278P/T296P; xxviii. S8P/G22D/T41I/N49S/A68T/N103I/S113N/P227L/S278P/T296P/N301R; xxix. S8P/G22D/T41I/N49S/A68T/N103I/S113N/P227L/D249K/S278P/T296P/N301R; xxx. S8P/G22D/T41I/N49S/A68T/S113N/P227L/D249K/S278P/T296P/N301R; xxxi. S8P/T41I/N49S/S57N/A68T/S113N/P227L/D249K/S278P/T296P/N301R; xxxii. S8P/G22D/T41I/N49S/A68T/S113N/P227L/D249K/S278P/N301R; xxxiii. S8P/T41I/N49S/A68T/S92T/S113N/P227L/D249K/V403D/T462I; xxxiv. S8P/G22D/T41I/N49S/A68T/S92T/S113N/P227L/D249K/V403D/T462I; xxxv. S8P/T41I/N49S/A68T/S92T/S113N/P227L/D249K/S411F; xxxvi. S 8P/G22D/T41I/N49S/A68T/S 92T/S113N/P227L/D249K/S411F; xxxvii. S8P/G22D/T41I/N49S/A68T/S92T/S113N/S196T/P227L/D249K/T255P/S278P/T296P/N301R/E325K/S411F; xxxviii. S8P/T41I/N49S/A68T/S92T/S113N/S196T/P227L/D249K/T255P/S278P/T296P/N301R/E325K/V403D/S411F/T462I; and xxxix. S8P/G22D/T41I/N49S/A68T/S92T/S113N/S196T/P227L/D249K/T255P/S278P/T296P/N301R/E325K/V403D/S411F/T462I.