Patent ID: 7638306

Claim:
A method of increasing the production of an biologically active compound in an Aeromicrobium erythreum cell wherein the biologically active compound is derived from (2S)-methylmalonyl-CoA, the method comprising the step of inhibiting transcription of the cob(1)alamin adenosyltransferase gene by insertional mutagenesis in the cob(1)alamin adenosyltransferase gene in an Aeromicrobium erythreum cell; wherein the biologically active compound is selected from the group consisting of an immunosuppressant, an anti-fungal agent, an anti-parasitic agent, an antibiotic, and an animal feed promotant; and wherein the production of the biologically active compound is increased when compared to production of the same biologically active compound by a corresponding cell wherein the activity of methylmalonyl-CoA mutase is not inhibited.