Patent ID: 8466266

Claim:
A compound of a structure according to Formula I: wherein X a is a stabilizing moiety which is a member selected from fluoroalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl; L l , L 2 , and L 3 are linkers independently selected from substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl; Y a is a member selected from CR a , and N wherein R a is a member selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl; Z a is a member selected from a solid support, OR b and NR b R b′ wherein R b and R b′ are independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl; Q is a quencher of fluorescent energy comprising a member selected from: (a) at least three residues, each independently selected from substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl wherein a first said residue is covalently linked to a second said residue via a first exocyclic diazo bond and a member selected from said first residue and said second residue is covalently linked to the third residue through a second diazo bond; and (b) at least two residues, each independently selected from substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl wherein at least two of said residues are covalently linked via an exocyclic diazo bond, with the proviso that at least one said residue is a member selected from substituted or unsubstituted polycyclic aryl and substituted or unsubstituted polycyclic heteroaryl groups; R c is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl and a phosphorus-containing linker covalently bound to a nucleic acid.