Patent ID: 8920558

Claim:
A method of crystallizing a purified protein, the method comprising: providing a purified protein to be crystallized, the purified protein being thermostable T1 lipase having been isolated from Geobacillus sp. strain T1; preparing a protein solution comprising the purified protein, a buffer solution having a working pH of at least 8.5, and between 3-5% glycerol, wherein the concentration of the protein is at least 2 mg/ml; preparing a reservoir solution comprising 0.1M MES, 0.1M NaH 2 PO 4 , 0.1M KH 2 PO 4 , and 1 M NaCl, and having a working pH of 6.5 or 6.6; combining the protein solution and the reservoir solution to obtain a protein droplet; and growing protein crystals from the protein droplet by hanging-drop vapor diffusion; wherein said protein crystals are grown in a microgravity environment for at least eight days at a temperature of at least 20° C. using High Density Protein Crystal Growth (HDPCG) crystallization hardware.