Patent ID: 7183076

Claim:
A method of preparing a bispecific antibody that specifically binds to a first and a second antigen, wherein said first and second antigens are different from one another, said method comprising: (a) providing a first nucleic acid molecule encoding a first polypeptide comprising the variable region of a light chain of an antibody that binds said first antigen; (b) providing a second nucleic acid molecule encoding a second polypeptide comprising the variable region of a heavy chain of said antibody that binds said first antigen; (c) identifying and/or providing a third nucleic acid molecule encoding a third polypeptide comprising the variable region of a heavy chain, which, when paired with the light chain of said antibody that binds said first antigen, specifically binds to said second antigen; (c) culturing a host cell comprising said first, second, and third nucleic acid molecules under conditions that permit formation of said bispecific antibody; and (d) recovering said bispecific antibody.