Patent ID: 8367059

Claim:
A method for cryopreservation of HCG-cell construct, said method comprising providing a porous HCG scaffold in a perfusion bioreactor, wherein said perfusion bioreactor comprises perfusion chambers that contain said HCG scaffold; seeding cells in said HCG scaffold and perfusing cell culture media through said bioreactor to produce said HCG-cell construct; removing said cell culture media and optionally washing said HCG-cell construct with a physiologically acceptable wash solution; perfusing said HCG-cell construct with a cryopreservation fluid using a transverse flow of said cryopreservation fluid through said HCG-cell construct in said bioreactor; removing HCG-cell construct from said bioreactor and freezing said HCG-cell construct in a cryopreservation fluid at increasingly colder temperatures over a period of time; and optionally storing said frozen HCG-cell construct at a suitable cryogenic temperature.