Patent ID: 7056706

Claim:
A method of sequencing a nucleic acid template comprising: (a) providing a plurality of first primers, each first primer comprising (i) a region of fixed nucleotide sequence having a defined length from about 4 bases to about 25 bases long and (ii) a region of randomized nucleotide sequence having a defined length of at least 2 bases long located 5′ to, 3′ to, or flanking the region of fixed nucleotide sequence, and wherein the region of fixed nucleotide is identical in sequence in each primer within the first primers; (b) annealing the plurality of first primers to different locations on a nucleic acid template, wherein at least one primer from within the plurality of first primers anneals specifically to the template; (c) extending the specifically annealed first primer from step (b) with a mixture of dNTPs and ddNTPs to generate a series of nucleic acid fragments; and (d) determining the nucleotide sequence of a first region of the template from the series of nucleic acid fragments.