Patent ID: 7332331

Claim:
A plasmid shuttle vector replicable in both Brevibacillus choshinensis and Escherichia coli , wherein said plasmid shuttle vector comprises the following DNA sequences: (1) an origin sequence of replication functionable in Escherichia coli ; (2) an origin sequence of replication functionable in Brevibacillus choshinensis; (3) a P2 promoter sequence; (4) a lac operator sequence; (5) an SD1 sequence; (6) an SD2 sequence; (7) a secretion signal sequence; (8) a multicloning site sequence; (9) an HS gene sequence; (10) a Rep protein gene; and (11) one member selected from the group consisting of the following members (a) and (b): (a) a drug resistance gene functionable as a selective marker in Brevibacillus choshinensis , and a drug resistance gene functionable as a selective marker in Escherichia coli ; and (b) a zeocin resistance gene, wherein a region comprising the DNA sequences (3)-(9) has a DNA sequence according to SEQ ID NO: 22.