Patent ID: 8158391

Claim:
A method of using a genetically modified Candida strain for the production of an α-carboxyl-ω-hydroxy fatty acid having a carbon chain length in the range from C6 to C22 wherein the genetically modified Candida strain is characterized by a first genetic modification class, a second genetic modification class, and a third genetic modification class; wherein the first genetic modification class comprises disruption of all copies of POX 4 and all copies of POX 5 in the genetically modified Candida strain, the second genetic modification class comprises one or more genetic modifications that collectively or individually disrupt a fatty alcohol oxidase in the genetically modified Candida strain, and a third genetic modification class comprising an insertion of a first gene into the Candida genome, wherein the first gene encodes: (i) a cytochrome P450, (ii) a desaturase, (iii) a lipase, (iv) a glycosyl transferase or (vi) a fatty alcohol oxidase that is not identical to a naturally occurring gene in Candida , the method comprising: fermenting the genetically modified Candida strain in a culture medium comprising a nitrogen source, an organic substrate having a carbon chain length in the range from C6 to C22, and a cosubstrate thereby oxidizing the organic substrate to the α-carboxyl-ω-hydroxy fatty acid.