Patent ID: 8735065

Claim:
A method for performing epigenetic analysis of native chromatin in a channel, comprising: (a) flowing the native chromatin through said channel, wherein said native chromatin is labeled with a plurality of labels, at least one of which is specifically complexed with an epigenetic marker on said native chromatin, and at least one other label is complexed with a protein and/or nucleotide of said native chromatin; (b) illuminating the channel using one or more light sources to create a plurality of interrogation volumes along said channel, wherein each interrogation volume is defined by walls of a submicrometer channel region that are separated by a width of less than about 1 μm of said channel and a beam of light; (c) detecting the at least one label and the one other label from the same or distinct interrogation volumes of said plurality to generate time-correlated resolution of said at least one and said at least one other label; and (d) analyzing said time-correlated resolution, thereby performing said epigenetic analysis.