Patent ID: 7094551

Claim:
A non-competitive immunoassay for detecting an FGF-23 protein molecule in a liquid sample suspected of containing molecules of FGF-23, wherein the FGF-23 protein molecule has an amino acid sequence selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 2, said immtmoassay comprising the steps of: (1) contacting said liquid sample with (a) a first capture antibody, said first antibody having a specificity for a first amino acid sequence present upon the FGF-23 protein molecules wherein the first amino acid sequence includes at least one glycosylated amino acid residue therein, (b) a second antibody, said second antibody having a specificity for a second amino acid sequence present upon the FGF-23 protein molecule; wherein said specificity of said first antibody is dissimilar to said specificity of said second antibody such that in use, said first and second antibodies are capable of sequentially or simultaneously binding to an FGF-23 molecule; and (2) detecting complexes of said FGF-23 molecule bound to said first antibody and bound to said second antibody.