Patent ID: 7923203

Claim:
A method of treating a bone defect in an individual comprising the steps of: obtaining a physiological fluid comprising nucleated cells, platelets, plasma and red blood cells, which has not been previously subjected to centrifugation, preparing a cell concentrate from the physiological fluid comprising combining a hypotonic fluid with the physiological fluid, the hypotonic fluid being in an amount which does not cause the cells to lyse in the physiological fluid/hypotonic fluid combination, subjecting the combined physiological fluid/hypotonic fluid to a first filtration device to produce a filter retentate, which is a first product, and a permeate fluid, wherein said filter retentate comprises platelets and nucleated cells per unit volume greater than in the physiological fluid, and wherein said permeate fluid comprises plasma and red blood cells, removing the filter retentate from the filter, and subjecting the filter retentate to a second filtration device to produce a cell concentrate, which is a second product, delivering the second product to the bone defect in the individual.