Patent ID: 7273714

Claim:
An assay for determining the level of alkylguanyltransferase(AGT) in a sample, comprising: (a) contacting the sample with a biotin-conjugated AGT binder under conditions permitting formation of a biotinylated AGT binder-AGT complex; (b) contacting the sample in (a) with an immobilized anti-AGT antibody, under conditions permitting the anti-AGT antibody to capture the biotinylated AGT binder-AGT complex; and (c) contacting the captured biotinylated AGT binder-AGT complex with streptavidin conjugated to either a peroxidase or a fluorophore under conditions permitting streptavidin to bind to the captured biotinylated AGT-complex; and (d) removing unbound streptavidin from streptavidin bound to the captured biotinylated AGT binder-AGT complex, wherein the amount of peroxidase or fluorophore bound to the captured biotinylated AGT binder-AGT complex is indicative of the level of AGT in the sample.