Patent ID: 7314763

Claim:
A fluidics-based assay device for detecting the presence or quantity of an analyte residing in a test sample, the device comprising: a reaction chamber for containing the test sample; detection probes that comprise particles that are conjugated with a first specific binding member for the analyte and labeled with a first detectable substance, the first detectable substance being capable of producing a detection signal; calibration probes that comprise magnetic particles labeled with a detectable substance second, the detectable substance second being capable of producing a calibration signal; wherein the first and second detectable substances are different non-fluorescent magnetic particles that are conjugated with a second specific binding member for the analyte; a channel in fluid communication with the reaction chamber, the channel defining a detection zone; and a magnetic device positioned adjacent to the detection zone; wherein the amount of the analyte within the test sample is directly or inversely proportional to the intensity of the detection signal produced at the detection zone calibrated by the intensity of the calibration signal produced at the detection zone.