Patent ID: 8153391

Claim:
A method of hydrolyzing an oil or fat comprising the steps of: (a) obtaining a composition comprising an oil or fat, wherein the oil or fat can be hydrolyzed by a polypeptide having a palmitase activity, wherein the palmitase activity comprises hydrolyzing at least 45% palmitic acid from the oil or fat, said polypeptide being selected from the group consisting of isolated, synthetic and recombinant polypeptides having a palmitase activity and said polypeptide either i. being encoded by a nucleic acid comprising a nucleic acid sequence having at least 85% sequence identity to full length SEQ ID NO:1, and wherein the nucleic acid encodes at least one polypeptide having a palmitase activity and the polypeptide having at least one base residue change described below in Table A, or ii. having at least 85% sequence identity to full length SEQ ID NO:2, and having at least one amino acid residue change described in Table A, TABLE A Amino Original New Acid Amino Amino Residue Acid Acid 27 G Q 45 G L 54 S H 61 D A E 62 V A G 72 R E K S T 74 F I 83 V M 96 A I 98 G L 116 E A F H L Q Q R, H140R R S S T V 117 L R, I161L 120 K L 133 S A 147 I F 151 I A G H 160 P T 172 R Q 212 S R 213 K T 214 T V N R Y 215 G A H 216 A V C L or iii. having at least 85% sequence identity to full length SEQ ID NO:2 but also comprising at least one of amino acid residue modification D61A; D61E; R72E; R72K; E116A; E116Q; E116R; E116T; E116V; S133A; 1151G; 1151A; V163R; D164R, (b) adding the polypeptide of step (a) to the composition comprising said oil or fat in an amount sufficient and under conditions sufficient to cause hydrolysis of the oil or fat, thereby hydrolyzing the oil or fat.