Patent ID: 8586361

Claim:
A plant cell having stably incorporated into its genome at least a first and a second transfer cassette, wherein said first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein said first and said second recombination sites are dissimilar and non-recombinogenic with respect to one another; said second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein said third recombination site is dissimilar and non-recombinogenic to said first and said second recombination site; and, said second recombination site is shared between the first and the second transfer cassette, such that the genome of the plant cell comprises in the following order the first recombination site, the first nucleotide sequence of interest, the second recombination site, the second nucleotide sequence of interest, and the third recombination site, wherein said first, said second and said third recombination sites are selected from the group consisting of FRT1 (SEQ ID NO:39), FRT12 (SEQ ID NO:21), and FRT87 (SEQ ID NO:24).