Patent ID: 8168404

Claim:
A method of selecting a patient for treatment of a cancer with 10-propargyl-10-deazaaminopterin, the method comprising the steps of: (a) obtaining a sample of the patient's cancer tissue; (b) determining the expression level of at least one selected polypeptide expressed in the sample; (c) comparing the determined expression level in the sample with a reference expression level for the same at least one polypeptide; and (d) selecting the patient for treatment 10-propargyl-10-deazaaminopterin where the comparison of the expression level in the sample of the at least one polypeptide and the corresponding reference expression levels indicate sensitivity to 10-propargyl-10-deazaaminopterin, wherein the at least one selected polypeptide is selected from the group consisting of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT).