Patent ID: 8298787

Claim:
A method for improving a secretion efficiency of a foreign protein, comprising the steps of: (a) transforming a yeast host with a recombinant foreign gene construct comprising a galactose-inducible promoter, a secretion signal sequence and a gene encoding the foreign protein to construct a transformed yeast host; and (b) culturing the transformed yeast strain under a condition that the activity of the galactose-inducible promoter is controlled, wherein the controlling of the activity of the galactose-inducible promoter is achieved by decreasing a transport rate of galactose from a culture medium into the transformed yeast host; and wherein the decreasing the transport rate of galactose from the medium into the transformed yeast host is achieved by rendering a galactose permease gene in the transformed yeast defective or partially disrupting said galactose permease gene to be non-functional.