Patent ID: 7608414

Claim:
A method for distinguishing between complicated and non-complicated Crohn's disease in a subject, the method comprising, providing a test sample from a subject diagnosed with Crohn's disease; detecting a level of an anti-Glc(β1,3)Glc(β) antibody (ALCA) in said sample by binding of said ALCA in said sample to a carbohydrate reagent comprising an isolated Glc(β1,3)Glc(β) glycan; detecting a level of an anti-GlcNAc(β1,4)GlcNAc(β) antibody (ACCA) in said sample by binding of said ACCA in said sample to a carbohydrate reagent comprising an isolated GlcNAc(β1,4)GIcNAc(β) glycan; detecting a level of an anti-mannan antibody (ASCA) in said sample by binding of said ASCA in said sample to a carbohydrate reagent comprising an isolated mannan; detecting a level of an anti-Man(α1,3)Man(α) antibody (AMCA) in said sample by binding of said AMCA in said sample to a carbohydrate reagent comprising an isolated Man(α1,3)Man(α); and distinguishing a complicated disease course in said subject by detection of an elevated level of each of said ALCA, ACCA, ASCA, and AMCA in said test sample relative to a control reference sample from one or more individuals known to have non-complicated Crohn's disease.