Patent ID: 7033774

Claim:
A method of producing antibodies specific to a glycosylated recombinant protein or peptide comprising the steps of: immunizing a mammal with a glycosylated recombinant protein or peptide; obtaining antibodies showing specificity to the glycosylated recombinant protein or peptide from the mammal; removing any antibody showing specificity to a native version of the recombinant protein or peptide from the antibodies; digesting the glycosylated recombinant protein or peptide with a peptidase to produce glycopeptides; treating the digested glycosylated recombinant protein or peptide with the antibodies; analyzing the treated digested glycosylated recombinant protein or peptide to identify glycopeptides that react with the antibodies; preparing quantities of the identified glycopeptides; and using the prepared identified glycopeptides as antigens to produce antibodies specific to the glycosylated recombinant peptide or protein.