Patent ID: 8067159

Claim:
A method comprising: providing a sample containing (1) at least one target nucleic acid molecule to be amplified and (2) constituents for enabling amplification of the at least one target nucleic acid molecule; loading the sample into a device; in the device, subdividing the loaded sample into a plurality of sample volumes, wherein at least one of said sample volumes contains said at least one target nucleic acid molecule; subjecting the sample volumes to a homogeneous assay for detecting amplified product, the homogeneous assay comprising: a single round of amplification of said at least one target nucleic acid molecule, wherein the single round of amplification yields a detectable amplified product of the at least one target molecule contained in the at least one of said sample volumes, and detection of amplified product in at least one of said sample volumes; and determining the number of said sample volumes that contain amplified product, wherein said sample volumes each have a volume of about 10 nl or less.