Patent ID: 8916380

Claim:
A method for preparing an embryonic stem cell (ESC)-like cell, which comprises the steps of: (a) providing a first cell population obtained from F1 hybrid B6D2F1 mouse ovarian tissue, wherein the first cell population comprises adult stem cells; (b) providing a second cell population which is embryonic fibroblast layer originated from an outbred ICR mouse fetus, wherein the second cell population is treated with mitomycin; (c) culturing the first cell population on the second cell population in a medium for a period of time sufficient to form a colony from the first cell population, wherein the culturing is carried out in the presence of at least 5,000 units/ml of leukemia inhibitory factor; and (d) subculturing a cell from the colony in a medium for a period of time sufficient to prepare the ESC-like cell, wherein the subculturing is carried out in the presence of 800-1,200 units/ml of leukemia inhibitory factor to obtain said ESC-like cells.