Patent ID: 8343439

Claim:
An assay device for performing an assay on a liquid sample using a detection conjugate capable of binding to an antigen and containing a label, said assay device comprising: a substrate surface comprising projections substantially perpendicular to said surface, said projections having a height, diameter and distance capable of generating lateral capillary flow of a fluid; a sample addition zone, a reaction zone and an absorbing zone, each of said zones being serially disposed along said substrate surface and connected by at least one fluid passage; a first functionality verifying feature located between the sample addition zone and the reaction zone; a second functionality verifying feature located within the absorbing zone, wherein both functionality verifying features are capable of undergoing a detectable change when contacted by the liquid sample; at least one alignment verification zone disposed on said substrate surface for verifying alignment of said assay device relative to a reader; at least one calibration zone disposed on said substrate surface and comprising a label in a known and defined amount.