Patent ID: 7141371

Claim:
A method for detecting and localizing one or more DNA mutations in a target DNA as compared to a reference DNA, the method comprising: a) using a restriction endonuclease(s) to digest a mixture of the reference and target DNA or to digest the reference and target DNA separately and then mix, wherein the restriction endonuclease(s) digest the reference and target DNA into different DNA fragments around the position of any mutation that causes the elimination or creation of a restriction site of the endonuclease(s): two short restriction fragments flanking the position of the mutation and a large fragment spanning the position of the mutation; b) labeling DNA strands flanking mutations by one or more cycles of denaturation, annealing and labeling with a DNA polymerase(s), wherein denaturation and annealing allow one single strand from the short fragments flanking a mutation to anneal with one strand of the large fragment spanning the mutation to form such a partially double-stranded DNA that a DNA polymerase(s) uses the short strand of this DNA as a primer and the long strand as a template to label the short strand DNA by incorporating modified nucleotides into newly synthesized DNA; c) hybridizing a combination of the above labeled DNA and a labeled control DNA to at least one DNA microarray, which microarray comprises spots comprising at least one DNA probe, at least some of the spots comprising a DNA probe from the target DNA or the reference DNA, or both; and d) identifying the sequence of at least one DNA from a spot that has significantly higher ratio of the label signal from the mixture of reference and target DNA to the label signal from the control DNA than at least one other spot on the microarray, wherein a labeled control DNA is (1) the reference DNA alone or (2) the target DNA alone treated in the same way as the mixture of reference and target DNA in steps a) and b), but labeled differently from the mixture of reference and target DNA, thereby detecting and localizing DNA mutations.