Patent ID: 7547510

Claim:
A method for amplifying deoxyribonucleic acid (DNA) for testing, comprising: i) adding Bacillus EA1 thermophilic proteinase to a sample comprising deoxyribonucleic nucleic acid (DNA) for testing, thereby forming a system, wherein the thermophilic proteinase digests protein in the sample and is capable of auto-catalysis at or above 90° C.; ii) closing the system; iii) incubating said sample in said system for a period at 65-80° C. as required to effect one or more of: lysis of one or more cells, digestion of one or more proteins, or digestion of one or more cell-wall enzymes, via activity of said thermophilic proteinase; iv) incubating the sample in said system at or above 90° C. to effect auto-catalysis of the thermophilic proteinase; v) opening the system; vi) providing polymerase chain reaction (PCR) reagents to the sample; and vii) amplifying the DNA for testing by PCR, wherein the protein and thermophilic proteinase are denatured in the system prior to opening without the addition of further denaturing agents.