Patent ID: 8283518

Claim:
A method of producing a transgenic chicken or quail comprising, administering to an artery leading to the ovary of a chicken or quail a composition comprising a transposon-based vector, wherein the transposon-based vector comprises a prokaryotic transposase gene operably linked to a first promoter, a modified Kozak sequence positioned 3′ to the first promoter so as to encode for at least the first codon of the transposase gene, and one or more genes of interest operably-linked to one or more additional promoters, wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon, and wherein the one or more genes of interest and their operably-linked promoters are flanked by transposase insertion sequences recognized by a transposase encoded by the transposase gene, such that the one or more genes of interest and their operably linked promoters and transposase insertion sequences are incorporated into a germ-line cell of the chicken or quail.