Patent ID: 6846839

Claim:
A method of identifying one or more indolinone compounds of Formula I that inhibit growth factor-stimulated cell proliferation comprising the following steps: (a) contacting cells with one or more indolinone compounds; (b) contacting said cells with one or more growth factors selected from the group consisting of VEGF, PDGF, and FGF; (c) monitoring an inhibitory effect on growth factor stimulated cell proliferation; and (d) identifying indolinone compounds of formula I that inhibit growth factor-stimulated cell proliferation, wherein, R 1 is H or alkyl; R 2 is O or S; R 3 is H; R 4 , R 5 , R 6 , and R 7 are each independently selected from the group consisting of hydrogen alkyl, alkoxy, aryl, aryloxy, alkaryl, alkaryloxy, halogen, trihalomethyl, S(O)R, SO 2 NRR′, SO 3 R, SR, NO 2 , NRR′, OH, CN, C(O)R, OC(O)R, NHC(O)R, (CH 2 ) n CO 2 R, CONRR′, and (CH 2 ) n ONRR′; A is selected from the group consisting of a 4,5,6,7-tetrahydroindole, thiophene, pyrrole, pyrazole, imidazole, 1,2,3-triazole, 1,2,4-triazole, oxazole, isoxazole, thiazole, isothiazole, 2-sulfonylfuran, 4-alkylfuran, 1,2,3-oxadiazole, 1,2,4-oxadiazole, 1,2,5-oxadiazole, 1,3,4-oxadiazole, 1,2,3,4,-oxatriazole, 1,2,3,5-oxatriazole, 1,2,3-thiadiazole, 1,2,4-thiadiazole, 1,2,5-thiadiazole, 1,3,4-thiadiazole, 1,2,3,4-thiatriazole, 1,2,3,5-thiatriazole, and tetrazole, wherein said group is optionally substituted with one or more substituents selected from the group consisting of alkyl, alkoxy, aryl, aryloxy, alkaryl, alkaryloxy, halogen, trihalomethyl, S(O)R, SO 2 NRR′, SO 3 R, SR, NO 2 , NRR′, OH, CN, C(O)R, OC(O)R, NHC(O)R, (CH 2 ) n CO 2 R, CONRR′, and (CH 2 ) n ONRR′; n is 0-3; R is selected from the group consisting of H, alkyl, and aryl; and R′ is selected from the group consisting of H, alkyl, and aryl, where the alkyl is optionally substituted with a six-membered heteroaliphatic ring, and where the six-membered ring is optionally substituted at one or more positions with substituents selected from the group consisting of alkyl, alkoxy, halogen, trihalomethyl, NO 2 , and (CH 2 ) n CO 2 R.