Patent ID: 8865420

Claim:
A method of diagnosing, prognosing or determining progression of a disease in a patient, said method comprising: (a) contacting a cell population from said patient with a potentiator; (b) determining the activation states of a plurality of potentiated signaling nodes in single cells from said potentiator contacted cell population, wherein each of said plurality of signaling nodes is different from the other signaling nodes and comprises a first activation state and a second activation state, wherein said determining step comprises: (i) contacting one or more cells of said cell population with a plurality of distinguishably detectable activation state-specific binding elements that are each specific for the first or second activation state of each different signaling node in said single cells; and (ii) detecting the presence or absence of binding of each of said distinguishably detectable activation state-specific binding elements to its corresponding activation state of the signaling node in said single cells from said cell population; (c) creating a response panel of said potential signaling nodes of said single cells comprising said determined activation states; (d) comparing the response panel in step c to a response panel of signaling nodes in cells for a known disease; and (e) diagnosing, prognosing or determining progression of said disease in said patient based on said comparison.