Patent ID: 6891086

Claim:
A method for the transformation of a Brassica plant cell comprising: introducing into plastids of said Brassica plant cell a construct comprising a promoter functional in a Brassica plant plastid operably associated with a nucleic acid sequence from Bacillus thuringiensis encoding a crystal protein providing for resistance to insects and a transcriptional terminator functional in a Brassica plant cell plastid, and flanking regions of homology to the Brassica plastid genome comprised of a pair of regions of a Brassica chloroplast genome of a size from about 1 kb to about 2.5 kb and which comprise or reside within a region identified by PCR amplification at 94 degrees C. for 4 minutes, 30 cycles of 94 degrees C. for 30 seconds, 50 degrees C. for 30 seconds, 72 degrees C. for 1 minute 45 seconds, with a final extension at 72 degrees C. for 7 minutes using SEQ ID NOS 3-4 and SEQ ID NOS 5-6 as primers, whereby said flanking regions cause the integration of the construct into the plastid genome of said Brassica plant cell, and selecting Brassica plant cells having plastids containing said construct.