Patent ID: 7687267

Claim:
A method for introducing a foreign substance into biological cells, the method comprising the steps of: a. introducing the foreign substance into a transfection device, wherein the transfection device has a first chamber, a second chamber, and a porous membrane separating the first and second chambers, wherein biological cells are attached to the porous membrane, and wherein the foreign substance is introduced to at least one chamber, and subsequently introduced into the biological cells via electroporation; b. introducing electrical conductive liquid to the transfection device such that the liquid fills the first and second chambers and form convex liquid droplets in and at vicinity of the chamber opening, c. introducing electrical conductive liquid to an electrode to be placed in each of the first and second chambers, to form convex liquid droplets on and at vicinity of the electrode, and d. placing the electrodes in the first and second chambers such that the convex liquid droplets on the electrodes are in contact with the convex liquid droplets on the first and second chamber openings, thereby establishing electrical connection between the electrodes.