Patent ID: 7736859

Claim:
A method for detecting an infection of Helicobacter pylori in a human subject, comprising: (a) incubating a stool sample from said human subject with no more than one first monoclonal antibody, or Fab-, F(ab)′2, Fv- or ScFv-fragment thereof, that specifically binds a first epitope of a Helicobacter pylori antigen that maintains its native immunogenic structure after intestinal passage through a human subject, wherein said first monoclonal antibody, or Fab-, F(ab)′2, Fv- or ScFv-fragment is labeled; (b) incubating said stool sample with no more than one second monoclonal antibody, or Fab-, F(ab)′2, Fv- or ScFv-fragment thereof, that specifically binds a second epitope of said Helicobacter pylori antigen, wherein said second monoclonal antibody, or Fab-, F(ab)′2, Fv-, or ScFv-fragment is bound to a solid phase and (c) detecting the formation of at least one antigen-antibody complex which is indicative of an infection of said Helicobacter pylori wherein the Helicobacter pylori antigen is selected from the group consisting of a urease, an oxidase, a catalase, a heat shock protein, an alkylhydroperoxide-reductase, a 20 kDa-protein (3-dehydroquinase type II), a 16.9 kDa-protein (neutrophil-activating protein), and a 33.8 kDa protein (fructose-bisphosphate-aldolase).