Patent ID: 7985572

Claim:
An isolated polypeptide having nitrilase activity with modified substrate acceptance, comprising at least one sequence selected from the group consisting of a) (K/R/H)XXXDXXGX(X*), (SEQ ID NO: 24) b) KAINDPVGH(X*), (SEQ ID NO: 9) c) GH(X*)SRPDV, (SEQ ID NO: 10) d) DP(A/V)GH(X*)SRPDV(L/T)(S/R)L, (SEQ ID NO: 11) and e) DPAGH(X*)SRPDVLSLLV, (SEQ ID NO: 12) wherein X* is not tyrosine and is localized at a position corresponding to position 296 in wild-type Alcaligenes faecalis nitrilase as described by SEQ ID NO: 2, and wherein said isolated polypeptide comprises an amino acid sequence having at least 80% identity to the amino acid sequence of SEQ ID NO: 2, 4, 6, or 8.