Patent ID: RE44437

Claim:
A method for detecting in a biological sample the presence or amount of a receptor complex comprising a phosphatidylinositol-3-kinase (PI3K) protein and a receptor, comprising: a. contacting with the sample a binding compound having a molecular tag attached thereto by a cleavable linkage and a cleaving probe having a cleavage inducing-moiety with an effective proximity, wherein the binding compound specifically binds either the PI3K or the receptor and the cleaving probe specifically binds an opposite member of the receptor complex from that bound by the cleaving probe; b. cleaving the cleavable linker of the binding compound within the effective proximity of the cleavage-inducing moiety of the cleaving probe, thereby releasing the molecular tag; and c. identifying the released molecular tag to determine the presence or amount of the receptor complex comprising the PI3K and the receptor in the sample.