Patent ID: 7148022

Claim:
An assay to determine the ability of a test substance to interfere with the association of protein 2C of a picornavirus or a protein 2C homolog from a calicivirus with dual-specificity kinase anchoring protein 2 (D-AKAP2), said assay comprising: i) providing a first component consisting of protein 2C of a picornavirus or a protein 2C homolog from a calicivirus; ii) providing a second component consisting of DAKAP2; iii) exposing said first component to a test substance followed by addition of said second component, or exposing said second component to said test substance followed by addition of said first component, or exposing said first component to said second component followed by said test substance; iv) incubating the mixture obtained in step (iii) for a time period and under conditions suitable to allow interaction between the components and/or the test substance; v) removal of any unassociated first or second component and/or test substance; and vi) detecting the presence, and optionally determining the amount, of associated first and second components.