Patent ID: 8128965

Claim:
A process of preparing a canola protein isolate, which consists essentially of the sequential steps of: (a) extracting a canola oil seed meal to cause solubilization of canola protein in said canola oil seed meal and to form an aqueous canola protein solution having a protein content of about 5 to about 40 g/L and a pH of about 5 to about 6.8 and residual canola oil seed meal; (b) separating said aqueous canola protein solution from said residual canola oil seed meal; (c) increasing the protein concentration of said aqueous canola protein solution to at least about 50 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a concentrated canola protein solution; and (d) drying said concentrated canola protein solution to provide a canola protein isolate having a protein content of at least about 90 wt % (N×6.25) on a dry weight basis and a canola protein profile which is about 25 to about 55 wt % of 2S canola protein, about 45 to about 75 wt % of 7S canola protein and about 0 to about 15 wt % of 12S canola protein.