Patent ID: 8309360

Claim:
A method for analyzing urine, comprising the steps of: preparing a measuring sample by fluorescent staining treatment for urine, wherein the fluorescent staining treatment damages a cellular membrane of yeast-like fungus in urine without substantially damaging a cellular membrane of erythrocyte in urine, and stains erythrocyte, yeast-like fungus and sperm in urine so that a fluorescent intensity of yeast-like fungus becomes more intense than that of erythrocyte, and a fluorescent intensity of sperm becomes more intense than that of yeast-like fungus; obtaining scattered light information and fluorescence information from the measuring sample by irradiating the measuring sample with light; and differentiating sperm contained in the measuring sample based on the scattered light information and the fluorescence information, wherein the fluorescent staining treatment is performed using a fungus membrane damaging agent for damaging a cellular membrane of yeast-like fungus, a first dye for staining yeast-like fungus so that a fluorescent intensity of damaged yeast-like fungus becomes more intense than that of the erythrocyte, and a second dye for staining sperm so that a fluorescent intensity of sperm becomes more intense than that of the damaged yeast-like fungus, wherein the first dye and the second dye are selected from the group consisting of fluorescent dyes represented by the following formula (1), formula (2), and formula (3): wherein A 1 is an oxygen atom, a sulfur atom, a selenium atom, or a C(CH 3 ) 2 ; R 1 is a lower alkyl group; X is a halogen or perchloric acid; Y is a —CH═ or —NH—; m is 1 or 2; n or 0 or 1: B is (a) a phenyl group substitute with two lower alkoxy groups, a lower alkylamino group, a di-cyanoamino group or a lower alkylcyanoamino group, or (b) a residue represented by the following formula (4) or formula (5): wherein A 2 is an oxygen atom, a sulfur atom, or a —C(CH 3 ) 2 —; R 2 is a lower alkyl group, wherein a carbon number of R 1 of the second dye is more than a carbon number of R 1 of the first dye.