Patent ID: 7534872

Claim:
A method for synthesizing and purifying an oligonucleotide immobilized on a solid support complex, comprising: a) providing: i) a polymer support comprising a long-chain alkyl amine, and ii) four pools of deoxyribonucleoside 3′-phosphoramidite monomers in solution, wherein at least one of said monomers contains 9-fluorenylmethoxycarbonyl as an amino protecting group on the nucleotide base; b) sequentially contacting said polymer support with a solution of monomers from one or more of said four pools under conditions such that a sequence of monomers is immobilized on said polymer support to generate an oligonucleotide/polymer support complex, wherein said complex comprises at least one monomer containing 9-fluorenylmethoxycarbonyl and wherein said solution, after said contacting, comprises unreacted material; c) treating said complex under conditions such that said unreacted material is substantially removed, thereby creating a purified oligonucleotide/polymer support complex, and d) treating said purified oligonucleotide/polymer support complex with DBU under conditions whereby said 9-fluorenylmethoxycarbonyl is released and said oligonucleotide is immobilized on said solid support.