Patent ID: 7939289

Claim:
A method for determining catch capacity of proteins from a tissue sample for the purpose of distinguishing the capacity of initiating the catch state where myosin, a protein that constitutes muscles, or filaments containing myosin bind to actin due to the contribution of twitchin that constitutes thick filaments along with myosin, while a certain tension is sustained, the method for the catch capacity of proteins designed to distinguish the catch capacity comprising the steps of: preparing synthetic thick filaments containing myosin and twitchin from the extract of said muscles from the tissue sample with a high salt buffer solution which has a salt concentration equal to the concentration of 10 mM phosphate buffer containing 0.4 M KCl, 4 mM MgCl 2 , 4 mM ATP, 4 mM EGTA and 2 mM DTT adjusted to be pH 7.0 by fractionation operation; mixing the synthetic thick filament and actin filaments; performing a catch treatment on the synthetic thick filaments; and determining the catch capacity of the proteins by detecting the binding of actin filaments to the synthetic thick filaments.