Patent ID: 8663924

Claim:
A method for determining an efficiency of target enrichment from a DNA library, comprising: adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and a positive control sequence from the DNA library wherein the negative control sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:5, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12 and SEQ ID NO: 13; determining, using quantitative PCR (polymerase chain reaction), a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence in the DNA library; performing enrichment of a target sequence from the DNA library using at least one bait sequence to produce a post-capture library; determining, using quantitative PCR, a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and determining the efficiency of the target enrichment, based on a ratio of the post-capture amount of the positive control sequence over the post-capture amount of the negative control sequence, or based on comparing (i) a first ratio of the pre-capture amount of the positive control sequence and the pre-capture amount of the negative control sequence, and (ii) a second ratio of the post-capture amount of the positive control sequence and the post-capture amount of the negative control sequence.