Patent ID: 7129060

Claim:
A process for presenting a passenger peptide or polypeptide on the surface of Gram-negative host bacteria, comprising a) providing a host bacterium transformed with a vector encoding a polynucleotide operatively linked to a promoter, wherein said polynucleotide comprises: (i) a nucleotide sequence encoding a signal peptide, (ii) a nucleotide sequence encoding a passenger peptide or polypeptide, (iii) a nucleotide sequence encoding a protease recognition site, (iv) a nucleotide sequence encoding a transmembrane linker, and (v) a nucleotide sequence encoding a transporter domain of the Adhesin Involved in Diffuse Adherence (AIDA) protein of E. coli , wherein the nucleotide sequence encoding the transporter domain is located downstream from the nucleotide sequence encoding the passenger peptide or polypeptide; and b) cultivating the host bacterium under conditions for inducing expression of the polynucleotide and presentation of the passenger peptide or polypeptide of (ii) on the surface of the host bacterium, wherein the passenger peptide or polypeptide of (ii) is heterologous in relation to the transporter domain of (v), and the host bacterium is homologous in relation to the transporter domain of (v).