Patent ID: 7582427

Claim:
A method for predicting the level of enzymatic activity of a UGT1A1 or UGT1A6 molecule in 2-amino-5-nitro-4-trifluoromethylphenol drug glucuronidation in a human subject, comprising: a) obtaining a nucleic acid sample from said human b) detecting a mutation in the exon 5 region of the UGT1A1 or UGT1A6 molecule without conducting an assay for each of the isoforms, that corresponds to nucleotide number 1456 in the genetic sequence of UGT1A1 which encodes an amino acid at position 486 in the amino acid sequence of UGT1A1 molecule and c) determining that, when the mutation in the exon 5 region encoding a UGT1A1 or UGT1A6 molecule is detected, the level of enzymatic activity of the UGT1 molecule in drug glucuronidation in the human subject would be lower than that of a UGT1 molecule without the mutation; wherein the drug glucuronidation is glucuronidation of 2-amino-5-nitro-4-trifluoromethylphenol.