Patent ID: 7148032

Claim:
A method of producing a phospholipase which comprises processing an expressed fungal peptide so as to cleave off a peptide from the C-terminal end and/or a peptide from the N-terminal end to obtain a core peptide with phospholipase activity, wherein the core peptide comprises a) the amino acid sequence given by amino acids 146–153 of SEQ ID NO: 1, amino acids 87–94 of SEQ ID NO: 3, or amino acids 79–86 of SEQ ID NO: 12; or a sequence identical to any of these amino acid sequences except for the substitution of a single amino acid with another amino acid; and b) at least two cysteine residues located on the N-terminal side of the sequence given in a); and c) at least two cysteine residues located on the C-terminal side of the sequence given in a).