Patent ID: 8663925

Claim:
A method of preparing a reaction mixture for Polymerase Chain Reaction (PCR) assay, comprising providing a first reagent solution comprising a least one substance selected from the group consisting of a polymerase, a primer, a plurality of dNTPs, a fluorescent qPCR dye, and a probe required for performing said assay, providing a second reagent solution comprising at least one other substance selected from the group consisting of a polymerase, a primer, a plurality of dNTPs, a fluorescent qPCR dye, and a probe not in the first reagent solution required for performing said assay, mixing the first and second reagent solutions for providing a mixed solution to be subjected to the PCR process, wherein the first reagent solution contains first colorant providing the first reagent solution a first color in the visible spectrum, the second reagent solution contains second colorant providing the solution a second color different from the first color and distinguishable in the visible spectrum, said mixing yields a mixed solution having, due to said first and second colorants, a third color different from the first and second colors and distinguishable in the visible spectrum.