Patent ID: 8569471

Claim:
A composition comprising: (a) a first polynucleotide comprising: (1) a gene switch/biosensor comprising a nucleic acid sequence encoding a physiological stimulus-sensitive chimeric transactivator, wherein said physiological stimulus-sensitive chimeric transactivator is oxygen-sensitive and comprises a GAL4 DNA-binding, domain (DBD), an oxygen-dependent degradation domain (ODD), and a p65 activation domain (p65 AD), and (2) an operatively linked tissue-specific promoter; and (b) a second polynucleotide comprising a nucleic acid sequence encoding a stem cell-attracting chemokine, wherein said second polynucleotide further comprises a GAL4 upstream activating sequence (UAS) linked to said nucleic acid sequence of said second polynucleotide, and wherein in response to hypoxia, said transactivator binds to the GAL4 UAS, resulting in expression of said nucleic acid sequence encoding said stem cell-attracting chemokine.