Patent ID: 7939256

Claim:
A method for detecting incorporation of at least one NTP into a single primer nucleic acid molecule, said method comprising: i. immobilizing onto a support a polymerase nucleic acid complex comprising a target nucleic acid, a primer nucleic acid which complements a region of the target nucleic acid, and at least one family A or family B polymerase, wherein said polymerase has an attachment complex that attaches to said polymerase on both sides of a DNA binding cleft and that increases processivity of said polymerase; ii. contacting said immobilized complex with at least one type of labeled nucleotide triphosphate (NTP), wherein each NTP is labeled with a detectable label, and iii. detecting the incorporation of said at least one type of labeled NTP into a single molecule of said primer, while said at least one type of labeled NTP is in contact with said immobilized complex, by detecting the label of the NTP while said at least one type of labeled NTP is in contact with said polymerase nucleic acid complex.