Patent ID: 8785196

Claim:
A kit for preparing a tissue suitable for transplantation, the kit comprising: cells; and an electrical stimulator providing an electrical stimulus waveform that includes a number of consecutive D-type signal pulse bursts that repeat at a fixed interval of 1 second or longer between consecutive pulse bursts; wherein each of the number of consecutive D-type signal pulse bursts includes a D-type signal having a frequency of less than 910 Hz, a shorter negative voltage pulse length of between 100 microseconds (μsec) and 1000 μsec in duration, a longer positive voltage pulse length in excess of 1 millisecond in duration, and a pulse burst length of from about 100 milliseconds to about 1 second; and wherein the electrical stimulus waveform promotes proliferation, differentiation, or matrix production of the cells into a tissue suitable for transplantation.