Patent ID: 7585626

Claim:
A method for sequencing a target segment of nucleotides in a nucleic acid test sample, said method comprising: (a) amplifying said target segment of nucleotides using at least two oligonucleotide primers that flank the target segment, wherein at least one of the oligonucleotide primers comprise: i) a complementary region with a sequence of about 15 to about 25 nucleotides that hybridizes adjacent to the target segment of nucleotides; and ii) an extender region that includes a primer hybridization sequence wherein said extender region does not hybridize to the target segment of nucleotides and said extender region is not fully complementary to the segment of nucleotides adjacent to which the complementary region of the primer hybridizes in the target nucleic acids, with a proviso that the length of the complimentary region and the extender region excluding the primer hybridization sequence portion of the extender region together represents a contiguous segment of at least, or, about 50 nucleotides; and (b) determining the sequence of the amplified target segment by priming with an oligonucleotide primer that is complementary to said primer hybridization sequence of the extender region.