Patent ID: 7902426

Claim:
A method for promoting efficiency of transformation of plant embryos, plant calli or cultured plant cells by Agrobacterium , which comprises: a) heating and centrifuging said plant embryos, plant calli or cultured plant cells; and b) contacting said plant cells or plant tissue with the bacterium so that a gene is transferred into the plant embryos, calli, or cells; wherein contact between the plant cells or plant tissue and the bacterium occurs after heating and centrifuging the plant cells or plant tissue, wherein heating is performed at a temperature of 37° C. to 52° C. for 1 minute to 24 hours, wherein said centrifuging is carried out under a centrifugal acceleration of 1000 G to 150,000 G for 1 second to 4 hours and wherein the efficiency of transformation is increased at least twice in comparison to the efficiency of transformation by Agrobacterium wherein the plant cells or plant tissues are not heated or centrifuged according to steps a) and b).