Patent ID: 8873827

Claim:
A computer-implemented method for determining a relationship between biomarker expression levels of morphological units in biological tissue and spatial distance from a morphological feature, the method comprising: identifying a morphological feature in an image of the biological tissue based on expression levels of a first biomarker indicative of the morphological feature; receiving a result of a segmentation analysis performed on the image of the biological tissue, the result identifying a first set of morphological units in the image external to the morphological feature, the first set of morphological units including cells and/or sub-cellular components; determining an expression level of a second biomarker corresponding to each unit in the first set of morphological units in the image of the biological tissue; determining a spatial distance between the morphological feature and each unit in the first set of morphological units; and automatically rendering, on a user interface displayed on a visual display device, a representation of a relationship between the expression levels of the second biomarker and the corresponding spatial distance from the morphological feature of the first set of morphological units wherein the representation of the expression levels of the second biomarker in the first set of morphological units includes overlaid renderings of: a representation of densities of the morphological units at different spatial distances from the morphological feature and at different expression levels of the second biomarker; and median expression levels of the second biomarker in the morphological units at different spatial distances from the morphological feature.