Patent ID: 8155889

Claim:
A method for assessing the degree of reliability of a nucleic acid base sequence, comprising the steps of: (A) computing a processing evaluation value E1 i for evaluating quality of measured data of an i-th base and/or contents of processing having been performed on the measured data of the i-th base when the measured data is processed into analytical data; (B) computing an analytical data evaluation value E2 i of the i-th base based on the processed analytical data; and (C) computing a degree of reliability by a predetermined calculation formula using the processing evaluation value E1 i and the analytical data evaluation value E2 i , wherein steps (A)-(C) are performed by a data processing computer, wherein in the step (A), an evaluation indicator E11 i defined by the following formula is computed as the processing evaluation value E1 i of an i-th base: E 1 i =E 11 i where E11 i is an evaluation indicator for evaluating a signal-to-noise ratio of measured data of an i-th base peak and is represented by the following formula: E 11 i =E 11max×(Signal( i )/Noise( i )/const E 11 where constE11 is a normalized constant, and when (Signal(i)/Noise(i))≧constE11, E11 i =E11max(<1), wherein in the step (B), a characteristic indicator for evaluating a peak-to-peak interval of the analytical data defined by the following formula is computed as the analytical data evaluation value E2 i of an i-th base: E 2 i =d Min i /d Max i where dMax i and dMini are a maximum value and a minimum value, respectively, among values of an interval d i(n) between two adjacent base peaks among a peak of the i-th base and base peaks near the peak of the i-th base, and when (dMin i /dMax i )>E2max, E2 i =E2max(<1), and wherein in the step (C), a degree of reliability QV i of an i-th base peak is computed by the following calculation formula: QV i =const QV ×Log 10 (Err i ) where constQV is a correction constant and Erri is an error probability of an i-th base and is represented by the following formula: Err i =−(1 −E 1 i )×(1 −E 2 i ) where E1 i and E2 i each takes a value between 0 and 1 and has Emax which is a maximum value less than 1.