Patent ID: 8268607

Claim:
A non-naturally occurring microbial organism, having a 1,3-butanediol (1,3-BDO) pathway, wherein said microbial organism comprises an exogenous nucleic acid encoding each of the following a 1,3-BDO pathway enzymes or proteins expressed in a sufficient amount to produce 1,3-BDO: 1) formate dehydrogenase that catalyzes the incorporation of CO2 into formate; 2) formyltetrahydrofolate synthetase that ligates formate to tetrahydrofolate to form 10-formyltetrahydro folate; 3) methenyltetrahydrofolate cyclohydrolase that converts 10-formyltetrahydrofolate to methyltetrahydrofolate; 4) methylenetetrahydrofolate dehydrogenase that converts methyltetrahydrofolate to methenyltetrahydrofolate; 5) methylenetetrahydrofolate reductase that converts metheneyltetrahydrofolate to 5-methyltetrahydrofolate; 6) methyltetrahydrofolate:corrinoid protein methyltransferase that catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to corrinoid iron sulfur protein; 7) corrinoid iron-sulfur protein; 8) nickel-protein assembly protein; 9) ferredoxin; 10) acetyl-CoA synthase that catalyzes the condensation of the methylated corrinoid iron sulfur protein, carbon monoxide and coenzyme A, yielding acetyl-CoA; 11) carbon monoxide dehydrogenase that converts CO and water to CO2 while passing the electrons to a reduced acceptor; 12) hydrogenase that transfers electrons from H2 to an acceptor; 13) acetoacetyl-CoA thiolase that converts acetyl-CoA to acetoacetyl-CoA; 14) acetoacetyl-CoA reductase (ketone reducing) that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA; 15) 3-hydroxybutyryl-CoA reductase (aldehyde forming) that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde; and 16) 3-hydroxybutyraldehyde reductase that converts 3-hydroxybutyraldehyde to 1,3-BDO.