Patent ID: 7429381

Claim:
A method of treating or identifying diseased tissues in a subject, comprising: (A) administering to said subject a bi-specific antibody or antibody fragment having at least one arm that specifically binds a targeted tissue and at least one other arm that specifically binds a targetable conjugate comprising at least two HSG haptens, wherein said at least one other arm that specifically binds a targetable conjugate comprises the light chain CDRs of an HSG-binding recombinant 679 antibody comprising the sequences KSSQSLFNSRTRKNYLG (SEQ ID NO:19), WASTRES (SEQ ID NO:20) and TQVYYLCT (SEQ ID NO:21) and heavy chain CDRs of an HSG-binding 679 antibody comprising the sequences IYTMS (SEQ ID NO:22), TLSGDGDDIYYPDSVKG (SEQ ID NO:23) and VRLGDWDFDV (SEQ ID NO:24); (B) optionally, administering to said subject a clearing composition, and allowing said composition to clear non-localized antibodies or antibody fragments from circulation; (C) administering to said subject a targetable conjugate which comprises a carrier portion which comprises or bears at least two HSG haptens and may comprise a diagnostic or therapeutic cation, and/or one or more chelated or chemically bound therapeutic or diagnostic agents, or enzymes; and (D) when said targetable conjugate comprises an enzyme, further administering to said subject 1) a prodrug, when said enzyme is capable of converting said prodrug to a drug at the target site, or 2) a drug which is capable of being detoxified in said subject to form an intermediate of lower toxicity, when said enzyme is capable of reconverting said detoxified intermediate to a toxic form, and, therefore, of increasing the toxicity of said drug at the target site, or 3) a prodrug which is activated in said subject through natural processes and is subject to detoxification by conversion to an intermediate of lower toxicity, when said enzyme is capable of reconverting said detoxified intermediate to a toxic form, and, therefore, of increasing the toxicity of said drug at the target site.