Patent ID: 7501504

Claim:
A method of making a labeled polynucleotide, comprising: a) contacting a double or single stranded nucleotide with a terminal deoxynucleotidyl transferase under conditions suitable to afford a 3′ hydroxyl terminus; and b) contacting with a compound of formula II: alone or in combination with a counterion thereof or a stable salt, solvate, clathrate or mixture thereof, wherein: base is wherein sugar is covalently bonded to N 1 of the base; wherein R 1 is —NHC(O)NH 2 ; wherein R 2 is —H or alkyl; each of X, Y, and Z is independently H, —OH, —O-alkyl, —SH, —SR 4 , —NHR 4 , —NR 4 R 5 , wherein R 3 is —H or metal; R 4 is —H or alkyl; R 5 is —H or alkyl; linker is (CH 2 CH 2 O) n CH 2 CH 2 , (CH 2 CH 2 O) n , (CH 2 O) n or (CH 2 ) n ; wherein n is an integer from 1 to 30; Q is O, S, or NH; and label is a colorimetric compound, a chemiluminescent compound, a bioluminescent compound, a fluorescent compound, a non- or weakly fluorescent compound, or a quencher, under conditions suitable for the attachment of said compound to a 3′ hydroxy terminus of said double or single stranded nucleotide to afford a biotin labeled polynucleotide.