Patent ID: 8034579

Claim:
A circularly permuted protein comprising: lipase B from Candida Antarctica (CALB); a linker sequence linking a native amino-terminal end of CALB and a native carboxy-terminal end of CALB; and a new amino-terminal end and a new carboxy-terminal end, wherein the new amino-terminal and carboxy-terminal ends of the circularly permuted protein are different from the native amino-terminal and carboxy-terminal ends of a corresponding native CALB protein, wherein the new amino-terminal end is located at an amino acid residue corresponding to an amino acid residue of the CALB protein SEQ ID No: 2 selected from: 144, 148, 150, 268, 277, 278, 283, 284, 289, and 294, wherein the circularly permuted protein comprises at least one improvement over the corresponding native CALB protein, wherein the improvement is selected from: increased activity, increased accessibility to the active site, increased flexibility of the active site, increased the enantioselectivity, and broader or changed substrate specificity.