Patent ID: 7381562

Claim:
A method for increasing the yield of Loblolly pine cotyledonary embryos, said method comprising the steps of: (a) culturing embryogenic Loblolly pine tissue on solid maintenance medium that does not comprise a gibberellin; (b) culturing the embryogenic pine tissue treated in accordance with step (a) in liquid maintenance medium that does not comprise a gibberellin; (c) culturing the embryogenic pine tissue treated in accordance with step (b) in a liquid maintenance medium comprising at least one gibberellin, provided that the liquid maintenance medium comprising a gibberellin does not comprise abscisic acid; and (d) culturing the embryogenic pine tissue treated in accordance with step (c) on solid development medium that does not comprise a gibberellin to yield a greater number of cotyledonary pine embryos than the number produced by culturing the embryogenic pine tissue in the liquid media of step (c) that does not comprise a gibberellin.