Patent ID: 8163477

Claim:
A method for detecting or quantitating an oligonucleotide in a bodily fluid or extract wherein the oligonucleotide is 8 to 50 nucleosides in length, comprising: creating a test sample by contacting the bodily fluid or extract with a probe, wherein: the probe is complementary to the oligonucleotide; and the probe comprises a detectable marker and a binding moiety, wherein the detectable marker and the binding moiety are each covalently bound to the probe; contacting the test sample with a solid support wherein the solid support comprises a binding partner of the binding moiety; contacting the test sample with a single-strand-specific nuclease; washing the test sample to remove detectable marker that is not associated with the solid support; detecting the presence or amount of detectable marker in the test sample; and thereby detecting or quantitating the oligonucleotide in the bodily fluid or extract.