Patent ID: 8329161

Claim:
A method of delivering an exogenous substance into a cell, comprising: (a) providing a red blood cell (RBC); (b) treating the RBC with a Ca 2+ -EGTA solution to obtain an isolated red blood cell membrane-derived vesicle (RDV), wherein the RDV is cytoskeleton free and has at least one erythrocyte protein present in the vesicle; (c) encapsulating the exogenous substance into the RDV to obtain an exogenous substance-loaded RDV; (d) contacting the cell with the exogenous substance-loaded RDV; and (e) allowing the cell to engulf the exogenous substance-loaded RDV to obtain a cell with the exogenous substance encapsulated within the RDV, thereby delivering the exogenous substance into the cell.