Patent ID: 7967441

Claim:
A method for illuminating an object to be imaged by a microscope, comprising: illuminating a first region of the object by primary light of a first spectral intensity distribution to generate secondary light of the second spectral intensity distribution; illuminating the object by secondary light of a second spectral intensity distribution, the secondary light arising from the scattering of the primary light; generating by means of a second region of the object another secondary light of a third spectral intensity distribution that differs from the second spectral intensity distribution; measuring the intensity of at least one wavelength of the another secondary light; comparing at least one of the measured intensity and a value derived from this measured intensity of the another secondary light to a threshold value; and activating a protective mechanism to protect the second region from further illumination when the at least one of the measured intensity and a value derived from this measured intensity of the another secondary light exceeds a threshold value.