Patent ID: 7297838

Claim:
A method of transforming and regenerating Flax plants comprising: growing a Flax anther culture-obtained callus on a high osmotic medium selected from the group consisting of 15-24% sucrose, 18-24% sucrose, 15-24% maltose, 6-9% sucrose+6-9% PEG, and 2×-4×A 22 -C salts for 2-14 days; transferring the anther culture-obtained callus to a low osmotic medium selected from the group consisting of ½-¼ A 22 -C salts and 1-6% sucrose; transforming the anther culture-obtained callus with a nucleic acid molecule by cocultivating the anther culture-obtained callus with Agrobacterium; growing the callus on a regeneration medium, said regeneration medium for regenerating shoots; growing the callus on an elongation medium for elongating the shoots; and transferring the shoots to a rooting medium for growing the Flax plant.