Patent ID: 7550265

Claim:
A process for removing a portion of a homopolymeric sequence from an analyte or library of analytes, said process comprising: (a) providing: (i) said analyte or library of analytes; (ii) A chimeric nucleic acid that comprises at least two nucleic acid segments, wherein the first segment and the second segment each comprise sequences complementary to different portions of a homopolymeric sequence in said analyte or library of analytes, wherein hybridization of said first segment to a first portion of said homopolymeric sequence in an analyte produces a first complex; wherein hybridization of said second segment to a second portion of said homopolymeric sequence in said analyte produces a second complex, and wherein the second portion of said homopolymeric sequence in said second complex is a substrate for a specific endonuclease, and wherein said first portion of said homopolymeric sequence in said first complex is resistant to said specific endonuclease. (iii) an endonuclease; (iv) reagents for hybridization and endonucleolytic digestion; (b) hybridizing the nucleic acid (ii) with said analyte or library of analytes (i) to form at least one second complex comprising the second segment in said nucleic acid (ii) hybridized to said portion of said homopolymeric sequence, and at least one complex comprising the first segment in said nucleic acid (ii) hybridized to a different portion of said homopolymeric sequence; (c) removing endonucleolytically said portion of the homopolymeric sequence of said analyte or library of analytes hybridized to said second segment in the second complex or complexes.