Patent ID: 7273712

Claim:
A method for detecting a composition that affects inflammation, the method comprising: a) adding the composition to a first cell culture, wherein the composition comprises a component with an unknown effect on inflammation; b) adding a glycated protein for inducing production of a determinant of inflammation to the first cell culture and a second cell culture; c) measuring an amount respectively from the first cell culture and the second cell culture of a produced determinant of inflammation, the first and second cell cultures respectively being endothelial cells, wherein the determinant of inflammation is selected from the group consisting of NF-κB, IL-1, IL-11, TNF-α, ICAM-1, or PAI-1; and d) comparing the amount of the determinant from the first cell culture to the amount of determinant from the second cell culture to determine whether the composition has a stimulating effect, an inhibitory effect, or no effect on the inflammation.