Patent ID: 8058027

Claim:
A method of enriching for a recombinant protein in cell culture, the method comprising: a) transfecting into a host cell, a first nucleic acid molecule comprising a nucleotide sequence encoding a recombinant protein and a second nucleic acid molecule comprising a nucleotide sequence encoding at least one anti-apoptotic protein selected from a protein comprising the amino acid sequence set forth in SEQ ID NO:6, 8 or 10 or a functional fragment thereof having anti-apoptotic activity, wherein both nucleotide sequences are operably linked to a DNA element comprising a nucleotide sequence set forth in SEQ ID NO:1, wherein the DNA element is capable of opening chromatin and/or maintaining the chromatin in an open state; and b) culturing the host cell under conditions such that the recombinant protein is produced in the presence of reduced levels of one or more contaminants selected from host cell protein and DNA, thereby to enrich for the recombinant protein in cell culture.