Patent ID: 7087744

Claim:
A fluorescent nucleotide represented by the formula: X—Y—Z wherein, X represents a residue of a natural or non-natural nucleotide, an oligonucleotide, or a polynucleotide, which binds to Y at a basic moiety of said residue; Y represents a divalent bridging group or a single bond; and Z represents a monovalent group derived from: a compound represented by the following general formula (II): wherein, R 1 , R 2 , R 3 , and R 4 each independently represents a substituted or unsubstituted alkyl group, and R 3 and R 4 may bind to each other to form a saturated or unsaturated ring; V 1 , V 2 and V 3 each independently represents a hydrogen atom or a substituent, V 1 and V 2 , and/or V 2 and V 3 may bind to each other to form a saturated or unsaturated ring; L 1 , L 2 , L 3 , L 4 , L 5 , L 6 and L 7 each independently represents a substituted or unsubstituted methine group; W represents an oxygen atom, a sulfur atom, —C(R 3 )(R 4 )— or —N(R 5 )— wherein R 3 , R 4 , and R 5 each independently represents a substituted or unsubstituted alkyl group; Q represents a nitrogen atom or C(V 7 ) wherein V 7 represents a hydrogen atom or a monovalent substituent and may bind to V 6 to form a saturated or unsaturated ring; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s represents 0 or 1; t represents 0 or 1; and u represents 0 or 1; and V 8 represents a hydrogen atom or a malevolent substituent, provided that, when W represents —N(R 5 )— or —C(R 3 )(R 4 )—, V 8 may bind to a substituent on W to form a saturated or unsaturated ring; or a compound represented by the following general formula (III): wherein, V 1 , V 2 , V 3 , R 1 , R 2 , R 3 , R 4 , L 1 , L 2 , L 3 , L 4 , L 5 , L 6 , L 7 , W, M, m, s, t, and u have the same meanings as defined above; E represents a nitrogen atom or —C(R 6 )═, where R 6 represents a hydrogen atom or a monovalent substituent; V 9 represents a hydrogen atom or a monovalent substituent and V 9 may bind to R 6 to form a saturated or unsaturated ring, and Z binds to Y at a reactive group existing in R 1 or R 2 .