Patent ID: 8394326

Claim:
A method comprising: obtaining a flow cytometer comprising: an interrogation flow cell comprising an examination zone, and a fluid handling system coupled to the interrogation flow cell, the fluid handling system comprising a sheath fluidic line for supplying a sheath fluid to the interrogation flow cell, and a plurality of assay fluidic lines, wherein each assay fluidic line comprises a proximal end and a distal end, and the distal ends of the assay fluidic lines are beveled and extend into the interrogation flow cell, wherein the beveled surfaces of the distal ends of the plurality of assay fluidic lines face each other; priming the interrogation flow cell of the flow cytometer with the sheath fluid; and injecting different assay fluids into a flow of the sheath fluid within the interrogation flow cell through separate assay fluidic lines of the plurality of assay fluidic lines.