Patent ID: 7045322

Claim:
A process for producing a lysosphingolipid by using a sphingolipid ceramide N-deacylase, the method comprising: a. preparing an aqueous solution containing sphingolipid and a sphingolipid ceramide N-deacylase that is soluble in the aqueous solution; b. adding at least one organic solvent which is immiscible with water to the aqueous solution prepared in step (a) and forming a two-phase system reaction mixture, wherein said organic solvent is selected from the group consisting of: (i) a hydrocarbon which is selected from the group consisting of, octane, decane, pentadecane, heptadecane, octadecane, 1-octene, 1-decene, 1-dodecene, methylcyclohexane, cyclodecane, toluene, xylene, mesitylene, n-ethylbenzene, n-butylbenzene, p-cymene, cumene, n-octyl chloride, and n-desyl chloride; (ii) an alcohol which is selected from the group consisting of 2-octanol, 1-decanol, and 1-dodecanol; (iii) an ester which is selected from the group consisting of isopropyl myristate, and n-butyl stearate; and (iv) an ether which is selected from the group consisting of phenyl ether, and diisopropyl ether; and; c. allowing the two-phase system reaction mixture formed in step (b) to stand by settling without dispersion for a period of time sufficient to produce a lysosphingolipid.