Patent ID: 6893847

Claim:
A process of amplifying Salmonella gene invA mRNA having a specific sequence, comprising obtaining a sample comprising Salmonella gene invA mRNA synthesizing cDNA employing an RNA-dependent DNA polymerase resulting in an RNA/DNA hybrid, digesting the RNA of the RNA/DNA hybrid with Ribonuclease H to produce a single-stranded DNA, producing a double-stranded DNA having a promoter sequence capable of transcribing RNA comprising said specific sequence or a sequence complementary to said specific sequence employing a DNA-dependent DNA polymerase, wherein said single-stranded DNA is the template for said producing and wherein said double-stranded DNA produces an RNA transcription product in the presence of an RNA polymerase, and synthesizing cDNA comprising annealing an oligonucleotide primer pair consisting of the sequence of SEQ ID NO: 4 and SEQ ID NO: 23 to said RNA transcription product and amplifying said cDNA by employing said RNA-dependent DNA polymerase, where either primer includes an RNA polymerase promoter sequence at the 5′ end.