Patent ID: 7189895

Claim:
A method of increasing isoflavonoid production in an isoflavonoid-producing plant, the method comprising: a) transforming a plant with (1) a first recombinant DNA construct comprising a polynucleotide selected from the group consisting of: (i) a polynucleotide encoding all or part of a flavanone 3-hydroxylase from the plant; (ii) a polynucleotide comprising a 5′ non-coding sequence, a 3′ non-coding sequence, or both, of an isolated nucleic acid fragment which encodes a flavanone 3-hydroxylase from the plant; or (iii) a polynucleotide comprising (i) and (ii); and (2) at least one second recombinant DNA construct comprising a polynucleotide encoding a maize C1 myb transcription factor and a maize R myc-type transcription factor; and b) growing the transformed plant of (a); and c) evaluating the plant or plant part obtained from the transformed plant for an increased quantity of isoflavonoid in the transformed plant or plant part as compared to the plant or plant parts obtained from an untransformed plant.