Patent ID: 7368234

Claim:
A method of determining the position of p n cloned nucleotide sequences within a genome, comprising: (a) providing a surface on which genomic DNA has been aligned using a molecular combing technique with the aid of a receding meniscus; (b) contacting the aligned genomic DNA with labeled probes, wherein the labeled probes comprise nucleotide sequences that are complementary to the cloned nucleotide sequences; (c) detecting hybridization between the labeled probes and the genomic DNA; and (d) determining the position of the labeled probes, the distance between the labeled probes, or the size of the labeled probes, and deducing therefrom the position of the p n cloned nucleotide sequences, wherein parts b) and c) are repeated “n” times by modifying the color, the labeling, or the mode of revealing the probes, so that if the number of colors, labelings, or modes of revealing equals “p”, it is possible to position p n cloned nucleotide sequences after “n” hybridizations, wherein the position of the labeled probes, the distance between the labeled probes, or the size of the labeled probes is converted into a histogram.