Patent ID: 8552170

Claim:
An expression vector comprising a cassette for expressing a drug selective marker gene containing an mRNA destabilizing sequence, at least one element for stabilizing gene expression and a cassette for expressing a gene encoding a recombinant protein, wherein the element for stabilizing gene expression consists of any one of the following (a) to (d) or any combination thereof: (a) a DNA sequence consisting of the region from the 41601st base to the 46746th base of the sequence of SEQ ID NO: 26; (b) a DNA sequence consisting of a partial sequence of the region from the 41601st base to the 46746th base of the sequence of SEQ ID NO: 26, wherein the partial sequence comprises the region from the 41601st base to the 42700th base of the sequence of SEQ ID NO: 26; (c) the DNA sequence of (a) or (b), wherein a recognition sequence of at least one restriction enzyme selected from the following group has been deleted: AscI, BsiWI, BssHII, BstBI (Csp45I, NspV), CpoI, CspI (RsrII), FseI, HindIII, MfeI, MluI, NotI, PacI, PaeR71, SgrA1, SphI, XbaI, XhoI, BclI, BglII, BlpI, EcoRI, SalI, SpeI, EcoR105I (SnaBI), EcoRV, NruI, PsiI, SmaI, SrfI, SacI, and BamHI; and (d) a DNA sequence consisting of the sequence complementary to the DNA sequence of (a), (b), or (c).