Patent ID: RE42704

Claim:
A method for producing biocompetent fibrinogen comprising: providing a first DNA segment encoding a secretion signal operably linked to a heterologous fibrinogen Aα chain, the DNA segment comprising genomic DNA encoding the Aα chain, a second DNA segment encoding a secretion signal operably linked to a heterologous fibrinogen Bβ chain, the DNA segment comprising genomic DNA encoding the Bβ chain, and a third DNA segment encoding a secretion signal operably linked to a heterologous fibrinogen γ chain, the DNA segment comprising genomic DNA encoding the γ chain, wherein each chain is from the same species, and wherein each of said first, second and third segments is operably linked to additional DNA segments required for its expression in the mammary gland of a host female mammal and the first, second, third segments are linked in a single vector ; introducing said DNA segments into a fertilized egg of a non-human mammalian species heterologous to the species of origin of said fibrinogen chains; inserting said egg into an oviduct or uterus of a female of said mammalian species to obtain offspring carrying said DNA segments; breeding said offspring to produce female progeny that express said first, second and third DNA segments and produce milk containing biocompetent fibrinogen encoded by said segments; collecting milk from said female progeny; and and recovering the biocompetent fibrinogen from the milk.