Patent ID: 7923214

Claim:
A solid support having immobilized thereon: 1) a chemiluminescent compound comprising a chemiluminescent moiety and a linking moiety and wherein the chemiluminescent moiety is selected from acridan esters, acridan thioesters, acridan sulfonamides, acridan ketenedithioacetal compounds, acridan compounds of the formula: wherein R 1 is selected from alkyl, alkenyl, alkynyl, aryl, and aralkyl groups of 1-20 carbon atoms any of which can be substituted with 1-3 groups selected from carbonyl groups, carboxyl groups, tri(C 1 -C 8 alkyl)silyl groups, a SO 3 − group, a OSO 3 −2 group, glycosyl groups, a PO 3 − group, a OPO 3 −2 group, halogen atoms, a hydroxyl group, a thiol group, amino groups, quaternary ammonium groups, or quaternary phosphonium groups, wherein X is selected from C 1 -C 8 alkyl, aryl, aralkyl groups, alkyl or aryl carboxyl groups having from 1-20 carbon atoms, tri(C 1 -C 8 alkyl)silyl groups, a SO 3 − group, glycosyl groups and phosphoryl groups of the formula PO(OR′)(OR″) wherein R′ and R″ are independently selected from C 1 -C 8 alkyl, cyanoalkyl, aryl and aralkyl groups, trialkylsilyl groups, alkali metal cations, alkaline earth cations, ammonium and trialkylphosphonium cations, wherein Z 1 is selected from O and S atoms, wherein R 6 is selected from substituted or unsubstituted C 1 -C 4 alkyl, phenyl, benzyl, alkoxyalkyl and carboxyalkyl groups, wherein 0 or 1 or 2 of the substituents R 7-14 are selected from alkyl, alkoxy, hydroxy, and halogen and the remaining of R 7-14 are hydrogen, 2) a first specific binding partner having specific binding affinity for an analyte, 3) an analyte specifically bound to the specific binding partner, and 4) an activator compound conjugate, which comprises an activator compound conjugated to a second specific binding partner having specific binding affinity for the analyte, wherein the activator compound conjugate is specifically bound to the analyte.