Patent ID: 8715953

Claim:
A method of producing an enoxaparin pharmaceutical composition, the method comprising: producing a batch of low molecular weight heparin (LMWH) by β-eliminative cleavage of a heparin preparation; exhaustively digesting a sample of the batch of LMWH with two or more heparin degrading enzymes; using a separation method to determine, in the sample that has been digested with two or more heparin degrading enzymes, the presence of a structural signature associated with the non naturally occurring sugar associated with peak 9 of FIG. 1 that results from β-eliminative cleavage; selecting the batch of LMWH as a batch of enoxaparin if the non naturally occurring sugar associated with peak 9 is present in the sample compared to a pre-selected value for enoxaparin; and formulating the selected batch of enoxaparin for medical use, thereby producing an enoxaparin pharmaceutical composition.