Patent ID: 8632970

Claim:
A method of capturing ten or more different nucleic acids of interest, the method comprising: providing a sample comprising or suspected of comprising the ten or more different nucleic acids of interest; providing a solid support having associated therewith a support capture probe, which support capture probe comprises only naturally occurring bases A, C, G, T, and/or U; providing ten or more subsets of n different target capture probes, which target capture probes comprise only naturally occurring bases A, C, G, T, and/or U, wherein n is at least two, wherein a different subset of target capture probes is provided for the different nucleic acids of interest, wherein the target capture probes in each subset are capable of hybridizing to nonoverlapping polynucleotide sequences in the corresponding nucleic acid of interest, and wherein the target capture probes in each subset are capable of hybridizing to the support capture probe; and hybridizing any nucleic acid of interest present in the sample to its corresponding subset of n target capture probes and hybridizing the subset of n target capture probes to the support capture probe, thereby capturing the nucleic acid on the solid support, wherein the hybridizing the subset of n target capture probes to the support capture probe is performed at a hybridization temperature which is greater than a melting temperature T m of a complex between each individual target capture probe and the support capture probe.