Patent ID: 7094542

Claim:
A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample, comprising the steps of: providing a biological sample containing nucleic acid from M. tuberculosis comprising a rpoB sequence; amplifying the rpoB sequence in an in vitro nucleic acid amplification reaction mixture comprising at least one polymerase activity, and primers consisting of SEQ ID NO:2 with SEQ ID NO: 3, to produce amplified nucleic acid containing a rpoB sequence; optionally fragmenting the amplified nucleic acid; hybridizing the amplified nucleic acid to at least one detection probe consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4 that hybridizes specifically to M. tuberculosis sequences; and detecting the amplified nucleic acid hybridized to at least one detection probe by detecting a label associated with the amplified nucleic acid.