Patent ID: 7192740

Claim:
A recombinant viral nucleic acid constructed from a virus selected from the group consisting of Eastern Equine Encephalomyelitis Viruses, Western Equine Encephalomyelitis Viruses, Rhinoviruses, Polioviruses, Simian Viruses and Adenoviruses, which possess a naturally occurring subgenomic promoter, the recombinant viral nucleic acid comprising: a first viral subgenomic promoter; a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter; a second viral subgenomic promoter; and a second nucleic acid sequence which is non-native to said virus and whose transcription is regulated by the second viral subgenomic promoter; wherein the first and second viral subgenomic promoters possess heterologous nucleic acid sequences relative to each other, and wherein the second viral subgenomic promoter is upstream of the first subgenomic promoter.