Patent ID: 6890726

Claim:
A method of identifying variant recombinases that mediate recombination at variant recombination sites, the method comprising, (a) bringing into contact a mutant recombinase, a first nucleic acid sequence comprising a first reporter gene and first and second recombination sites, wherein the first and second recombination sites are variant recombination sites, and a second nucleic acid sequence comprising a second reporter gene and third and fourth recombination sites, wherein the third and fourth recombination sites can be recombined by a non-mutant recombinase, (b) determining if recombination occurs between the first and second recombination sites, and determining if recombination occurs between the third and fourth recombination sites, wherein recombination between the first and second recombination sites indicates that the mutant recombinase is a variant recombinase that mediates recombination at variant recombination sites, wherein recombination between the third and fourth recombination sites indicates that the mutant recombinase retains the ability to mediate recombination at non-variant recombination sites.