Patent ID: 7235387

Claim:
A method for mutation analysis of the pol gene of HIV-1 isolates comprising the steps of: a) isolation of a sample, b) virion RNA extraction of the isolated sample material c) amplifying RNA via nested PCR using outer primers as represented in SEQ ID No.1 and 2 to obtain a primary PCR product, d) amplifying said primary PCR product via nested PCR using a 5′ and 3′ primer chosen from the inner primers as represented in SEQ ID No. 3, 4, 5 and 6, and e) sequencing this secondary obtained PCR product using at least one sequencing primer chosen from any of SEQ ID No. 7 to 12; wherein at least one of the sequencing primers is replaced by one or a pair of replacement primers having at least 90% similarity to SEQ ID No. 7 to 12 and wherein the replacement primers obtain a sequence from the region that the at least sequencing primer is expected to cover.