Patent ID: 7994399

Claim:
A method for generating a transgenic Zea mays plant, comprising the steps of: a. isolating an immature embryo of a Zea mays plant, b. co-cultivating said isolated immature embryo with a bacterium belonging to the genus Rhizobiaceae comprising at least one transgenic T-DNA, said T-DNA comprising at least one selectable marker gene, with a co-cultivation medium comprising silver nitrate and L-Cysteine, wherein the isolated immature embryos are placed on one or more layers of filter paper on the surface of agar co-cultivation medium, or on one or more layers of filter paper containing liquid co-cultivation medium solution in a plate without agar medium, c. transferring the co-cultivated immature embryo to a recovering medium comprising: i. an effective amount of at least one antibiotic that inhibits or suppresses the growth of the bacterium belonging to the genus Rhizobiaceae, ii. L-proline in a concentration from about 1 g/l to about 10 g/l, iii. silver nitrate in a concentration from about 1 μM to about 50 μM, and iv. an effective amount of at least one auxin compound, but not comprising an effective amount of a phytotoxic selection agent, d. inducing formation of embryogenic callus and selecting a transgenic callus on a medium comprising: i. an effective amount of at least one auxin compound, and ii. an effective amount of a selection agent allowing for selection of callus comprising the at least one transgenic T-DNA, and e. regenerating and selecting plants containing the at least one transgenic T-DNA from said transgenic callus