Patent ID: 8759115

Claim:
A method of conducting an assay, comprising: verifying the alignment of an assay device in a reader using at least one alignment verification zone disposed on the assay device; adding a sample and detecting at least one reaction between the sample and one or more reagents using the assay device, the assay device having at least one fluid passage, a sample addition zone, a reaction zone and an absorbing zone, said one or more reagents including a detection conjugate capable of binding to an antigen and containing a label; determining the presence of the label in a first area downstream from the sample addition zone in which the absence of signal or detection of an insufficient signal is taken as an indication of error; said method further comprising a calibration step, wherein the amount of at least one label, pre-dispensed in a zone for calibration on the assay device, separate from the fluid passage, is determined and the amount compared to a preset value.