Patent ID: 6893834

Claim:
A method for assaying presence, activity, or both, of an enzyme, the method comprising: (a) under conditions which render the enzyme active, reacting the enzyme with a corresponding substrate, the substrate including a binding moiety, for a time sufficient to yield phosphorylated product when assaying enzymes that catalyze transfer of a phosphate group to a lipid or phospholipid substrate, or dephosphorylated product when assaying enzymes that catalyze removal of a phosphate group from a phospholipid substrate, the product also including the binding moiety; and (b) contacting the product with a binding matrix specific for the binding moiety; whereby product is specifically fixed to the matrix; and then (c) analyzing the matrix for presence of, amount of, or both the presence and the amount of the product, whereby the presence, the activity, or both the presence and activity of the enzyme is determined.