Patent ID: 8089623

Claim:
A method of normalizing spectral sensitivity from an instrument, the instrument using a microplate having a plurality of reaction chambers, the instrument having a plurality of filters, the method comprising: testing a first reference standard deposited in a first reaction chamber of the plurality of reaction chambers in the microplate, wherein the first reference standard is operable for outputting a first reference standard signal in response to the testing; testing a sample aliquot deposited in a second reaction chamber of the plurality of reaction chambers, wherein the sample aliquot includes at least a portion of a polynucleotide sample, at least one dye is deposited in the second reaction chamber, and the at least one dye is operable for outputting a data signal representative of the polynucleotide sample in response to the testing; detecting the first reference standard signal and the data signal; generating a normalization bias based on analyzing the first reference standard signal relative to a predetermined level; and normalizing the data signal relative to the normalization bias.