Patent ID: 8693743

Claim:
A method for analyzing a tissue specimen comprising: obtaining a first digital tissue sample image showing a first tissue sample, wherein the first tissue sample is stained for a first biomarker, and wherein the first digital tissue sample image shows a first set of cells; obtaining a second digital tissue sample image showing a second tissue sample, wherein the second tissue sample is stained for a second biomarker, wherein the second digital tissue sample image shows a second set of cells, and wherein the second set comprises a subset of the first set of cells; analyzing the first digital tissue sample image to identify cells of the first tissue sample positive for the first biomarker; analyzing the second digital tissue sample image to identify cells of the second tissue sample positive for the second biomarker; identifying, on a per-cell basis, the subset of the first set of cells shown in the second digital tissue sample image; performing, by a computer processor, and based on the identifying, co-expression analysis of the cells of the first tissue sample positive for the first biomarker and the cells of the second tissue sample positive for the second biomarker to create a biomarker co-expression profile, wherein the co-expression analysis identifies any cell in the subset positive for both the first biomarker and the second biomarker, any cell in the subset positive for only the first biomarker, and any cell in the subset positive for only the second biomarker; and displaying the biomarker co-expression profile.