Patent ID: 8710208

Claim:
A kit for amplifying at least three target polynucleotides, the kit comprising: at least three species of reverse stem-loop ligation probes, wherein the at least three species of reverse stem-loop ligation probes vary from each other in the sequence of the 3′ target-specific portion, wherein the at least three species of reverse stem-loop ligation probes vary from each other in the sequence of their stem, wherein the at least three species of reverse stem-loop ligation probes vary from each other in the sequence of their loop, wherein the at least three species of reverse stem-loop ligation probes comprise an extension blocker, and wherein the at least three species of reverse stem-loop ligation probes comprise at least one degradable nucleotide within their 3′ target-specific portion, stem, or loop; and an agent capable of digesting the degradable nucleotide within the probes.