Patent ID: 7195918

Claim:
A method for producing a recombinant Actinobacillus succinogenes comprising: (a) providing a plasmid comprising at least one marker gene which confers resistance to an antibiotic comprising a Tn3 Amp R gene, or an ampicillin, tetracycline or chloramphenicol resistance gene operably linked to a first promoter comprising one or more pairs of −35 and −10 promoter elements of an Actinobacillus succinogenes pckA gene; an origin of replication comprising at least a 1.5 kB PstI-HaeII fragment of a pGZRS-1 replicon so as to be functional in Actinobacillus succinogenes ; a second promoter comprising one or more pairs of −35 and −10 promoter elements of the Actinobacillus succinogenes pckA gene; and a cloning site for a nucleic acid downstream of the second promoter; (b) transforming Actinobacillus succinogenes with the plasmid; and (c) selecting in the presence of the antibiotic the recombinant Actinobacillus succinogenes from non-transformed Actinobacillus succinogenes.