Patent ID: 7326555

Claim:
A method of purifying adenovirus particles from a cell culture medium comprising adenovirus particles which comprises: a) lysing host cells within the cell culture medium; b) selectively precipitating impurity DNA away from the adenovirus particles by adding a selective precipitation agent to the cell culture medium, wherein the selective precipitation agent is selected from the group consisting of domiphen bromide (DB), cetylpyridinium chloride (CPC), cetyltrimethylammonium bromide (CTAB), benzethonium chloride (BTC), tetradecyltrimethylammonium bromide or chloride (TTA), and polyethylene imine (PEI); c) clarifying the cell culture medium; and d) recovering purified adenovirus particles from the cell culture medium; wherein at least 80% of impurity DNA is precipitated away from the cell culture medium.