Patent ID: 7645592

Claim:
A method for detecting a soluble glycoprotein VI (GPVI) polypeptide or peptide in a sample, comprising the steps of: a. contacting the sample with a first monoclonal antibody specific for the GPVI polypeptide or peptide, b. capturing the GPVI polypeptide or peptide in the sample with the first monoclonal antibody, c. contacting the sample with a second monoclonal antibody specific for the GPVI polypeptide or peptide, and d. detecting the captured GPVI polypeptide or peptide with the second monoclonal antibody, wherein the first and second monoclonal antibodies inhibit collagen-induced platelet aggregation at an IC 50 of less than 7μg/ml, wherein the IC 50 is determined using a concentration of collagen that induces 70-90% platelet aggregation within 5 minutes of its contact with platelets in a platelet aggregation assay, and wherein the first and second monoclonal antibodies specifically bind to the GPVI polypeptide or peptide at a Kd of lower than 10 −8 M, and wherein the first and second monoclonal antibodies are different, and wherein the first monoclonal antibody and second monoclonal antibody are selected from OM1 (ATCC No. PTA-5938), OM2 (ATCC No. PTA-5939), OM3 (ATCC No. PTA-5940), and OM4 (ATCC No. PTA-5941).