Patent ID: 7989209

Claim:
A method of obtaining measurements of a sample from an image thereof, comprising: selecting a region of interest in the sample from an RGB image thereof by: selecting a positively contrasted region from a surrounding thereof in a marker-only image corresponding to the RGB image, the marker-only image being obtained through chromogen separation of the RGB image of the sample, the positively contrasted region of the marker-only image including at least one of a relatively larger nuclei and a relatively higher cell density than the surrounding; segmenting the region of interest in the RGB image to identify any objects of interest therein by: applying a low pass filter to the marker-only image of the sample obtained through chromogen separation of the RGB image of the sample; determining a marker-only histogram of pixels in the marker-only image; and binarizing the marker-only image according to a threshold in the marker-only histogram so as to form a mask for discriminating between negative and positive regions of the sample; implementing feature extraction to determine measurements for the identified objects of interest; and determining cell scores with respect to marker localization and signal to noise ratio.