Patent ID: 7572583

Claim:
An oligonucleotide primer for amplifying a random subset of restriction fragments obtained from a target DNA using at least one restriction endonuclease, wherein the restriction fragments are ligated to an adaptor sequence which is a double-stranded synthetic oligonucleotide (a) one end of which is designed for ligation to one end or both ends of the restriction fragments generated with said at least one restriction endonuclease, and (b) the other end of which cannot be ligated to said restriction fragments; and wherein said oligonucleotide primer comprises a nucleotide sequence selected from the group consisting of: a primer enabling amplification of restriction fragments ligated with a PstI adaptor: 5′-GACTGCGTACATGCAGacc-3′ (SEQ ID NO:6); a primer enabling amplification of restriction fragments ligated with a MseI adaptor: 5′-GATGAGTCCTGACTAAtac-3′ (SEQ ID NO:65); a primer enabling amplification of restriction fragments ligated with a TaqI adaptor: 5′-TGAGTCCTGACCGAacc-3′ (SEQ ID NO:39); a primer enabling amplification of restriction fragments ligated with a EcoRI adaptor: 5′-CTGCGTTACCAATTCaac-3′ (SEQ ID NO:45); a primer enabling amplification of restriction fragments ligated with a AseI adaptor: 5′-GACTGCGTACCTAATaac-3′ (SEQ ID NO:47); a primer enabling amplification of restriction fragments ligated with a Sse8387-I adaptor: 5′-GACTGCGTACATGCAGGaa-3′ (SEQ ID NO:66); and a primer enabling amplification of restriction fragments ligated with a ApaI adaptor: 5′-GACTGCGTACAGGCCCg-3′ (SEQ ID NO:64).