Patent ID: 8821856

Claim:
A method of delivering a polynucleotide encoding an antigen of interest to a dendritic cell, the method comprising: infecting the dendritic cell with a recombinant lentivirus, wherein the recombinant lentivirus comprises: (i) the polynucleotide encoding the antigen of interest and wherein the polynucleotide is operably linked to an expression control sequence, and wherein the polynucleotide further comprises an R sequence and an U5 sequence from a 5′ lentiviral long terminal repeat (LTR), and a self-inactivating lentiviral 3′ LTR, and (ii) a viral envelope comprising a fusogenic molecule which is a Sindbis virus E1 glycoprotein, and a cell-specific binding determinant which is an anti-DEC-205 antibody that (a) targets dendritic cells, (b) is separate from the fusogenic molecule, and (c) is incorporated into the envelope of the recombinant lentivirus.