Patent ID: 7147846

Claim:
A method for producing a prosthetic graft, comprising: applying one or more adherent cells to a porous prosthetic implant for containing blood in vivo, wherein the prosthetic implant has an outer surface that is not in contact with blood flow in vivo and an inner surface that is in contact with blood flow in vivo, the inner surface defining an interior space for containment of blood flow; wherein the adherent cells are applied to the outer surface, and not to the inner surface, of the porous prosthetic implant; and wherein the adherent cells are transfected with at least one recombinant nucleic acid molecule operatively linked to a transcription control sequence, the recombinant nucleic acid molecule encoding a protein that enhances patency of the prosthetic implant; and incubating the implant ex vivo under conditions sufficient to allow the adherence of the adherent cells to the outer surface of the implant.