Patent ID: 8236791

Claim:
A method of determining whether a compound is an inhibitor of Ca 2+ binding activation of a human S100 protein, the method comprising combining the compound with Ca 2+ and a biosensor of the S100 protein in an aqueous solution, wherein the biosensor comprises a human S100 protein that has a fluorescent dye covalently bound at an amino acid residue thereof which is at a three-dimensional position equivalent to Cys81 of S100A4, wherein upon the binding of Ca 2+ to the biosensor of the S100 protein the fluorescence of the fluorescent dye increases, wherein the compound is determined to be an inhibitor of Ca 2+ binding activation of the human S100 protein if the compound prevents increased fluorescence of the fluorescent dye upon exposure of the biosensor of the S100 protein to the Ca 2+ .