Patent ID: 7060808

Claim:
A first nucleic acid molecule encoding a humanized antibody heavy chain or a fragment thereof wherein the humanized antibody heavy chain comprises a human heavy chain constant region and a human heavy chain variable region, wherein CDR1, CDR2 and CDR3 of the heavy chain are substituted with the corresponding CDRs of monoclonal antibody 225 deposited under ATCC Accession No: HB 11935, and amino acid substitutions consisting at positions 24, 28, 29, 30, 48, 49, 67 and 71 according to the Kabat numbering system; or a second nucleic acid molecule encoding a humanized antibody kappa light chain or a fragment thereof wherein the humanized antibody kappa light chain comprises a human kappa light chain constant region and a human kappa light chain variable region, wherein CDR1, CDR2 and CDR3 of the light chain are substituted with the corresponding CDRs of monoclonal antibody 225, and an amino acid substitution consisting at position 49 according to the Kabat numbering system; wherein a protein encoded by the first nucleic acid molecule and the second nucleic acid molecule binds to human EGF-receptor.