Patent ID: 7995194

Claim:
A method for performing a target analyte immunoassay of a fluid sample, comprising the steps of disposing the fluid sample within a chamber; providing a detectable label within the sample, which label is operative to bind to the target analyte to produce labeled target analyte, which labeled target analyte is detectable in the sample; providing at least one first surface area within the chamber which first surface area has target analyte specific ligands attached to it, which target analyte-specific ligands are operative to selectively bind target analyte present in the sample to the first surface area; providing at least one second surface area in the chamber which is free of target analyte-specific ligands operative to selectively bind target analyte present in the sample to the second surface area; optically scanning the at least one first area to detect and record an average label signal intensity per pixel distribution of the labeled target analyte in the at least one first area; optically scanning the at least one second area to detect and record an average label signal intensity per pixel distribution of the labeled target analyte in the at least one second area; and determining at least one of an amount of labeled target analyte in the at least one first area, an amount of labeled target analyte in the at least one second area, and a ratio of labeled target.