Patent ID: 8859208

Claim:
A recombinant influenza viral system comprising: a) expression vectors comprising vector comprising a promoter operably linked to an influenza virus PA antisense cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus PB1 antisense cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus PB2 antisense cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus HA antisense cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus NP antisense cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus M antisense cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus NA antisense cDNA linked to a transcription termination sequence, and a vector comprising a promoter operably linked to an influenza virus NS antisense cDNA linked to a transcription termination sequence, and (b) protein expression vectors, comprising a vector comprising a promoter operably linked to a DNA segment encoding an influenza virus PA polypeptide, a vector comprising a promoter operably linked to a DNA segment encoding an influenza virus PB1 polypeptide, a vector comprising a promoter operably linked to a DNA segment encoding an influenza virus PB2 polypeptide, and a vector comprising a promoter operably linked to a DNA segment encoding an influenza virus NP polypeptide; wherein upon expression of vectors of (a) and (b) in a host cell, functional viral ribonucleoproteins (RNP) comprising the genomic vRNA segments of recombinant influenza virus are formed, and wherein said system does not comprise a helper virus.