Patent ID: 8486619

Claim:
An arrayed imaging reflectometry (AIR) sensor chip suitable for the detection of influenza immune responses comprising: a multilayer substrate comprising a surface and hemagglutinin polypeptides from different influenza strains or isolates, wherein said hemagglutinin polypeptides from each individual influenza strain or isolate comprise a hemagglutinin epitope and are covalently attached to the chip surface at different locations; wherein said hemagglutinin polypeptides retain conformational epitopes that are capable of binding to anti-influenza antibodies; wherein said surface with hemagglutinin polypeptides covalently attached at the different locations forms a coating on the chip surface that results in destructive interference of polarized light illuminating said surface at an appropriate incident angle and wavelength in the absence of antibody binding; and wherein exposure of the surface to a sample comprising anti-influenza antibodies produces a detectable change in reflectance at a location upon antigen-antibody binding.