Patent ID: 7790399

Claim:
A method for the preparation of human, humanized or chimæric antibodies or polypeptides having different binding profiles, comprising an Fc region of human IgG, wherein said method comprises: a. providing candidate human, humanized or chimæric antibodies or polypeptides comprising the Fc region of human IgG produced naturally by or following transfection with a vector comprising the coding sequence for said antibody or polypeptide into animal cell lines comprising, EBV-transformed human B cell lines or from eukaryotic microorganisms, b. testing the binding of said antibodies or polypeptides on Fcgamma receptors FcgammaRIIIA, FcgammaRIIA and FcgammaRIIB, and c. selecting antibodies or polypeptides which: ii. bind to FcgammaRIIA and FcgammaRIIB but do not bind to FcgammaRIIIA or bind to FcgammaRIIIA with an ID50 superior or equal to 0.2 microM.