Patent ID: 7067127

Claim:
An isolated N-acetylglucosamine-1-phosphotransferase comprising an α-subunit, a β-subunit and a γ-subunit, wherein the α-subunit comprises an amino acid sequence of SEQ ID NO:1 or an amino acid sequence having biological activity of an α-subunit of N-acetylglucosamine-1 -phosphotransferase and which is encoded by an isolated nucleic acid which hybridizes under stringent conditions to the complement of nucleotides 165 to 2948 of SEQ ID NO:4, the β-subunit comprises an amino acid sequence of SEQ ID NO:2 or an amino acid sequence having biological activity of a β-subunit of N-acetylglucosamine-1-phosphotransferase and which is encoded by an isolated nucleic acid which hybridizes under stringent conditions to the complement of nucleotides 2949 to 3932 of SEQ ID NO:4, and the γ-subunit comprises an amino acid sequence of SEQ ID NO:3 or an amino acid sequence having biological activity of a γ-subunit of N-acetylglucosamine-1-phosphotransferase and which is encoded by an isolated nucleic acid which hybridizes under stringent conditions to the complement of SEQ ID NO:5, wherein the stringent conditions comprise washing in 0.2×SSC and 0.1% SDS at 65° C.