Patent ID: 8518642

Claim:
A method of estimating whether a region of a first probe nucleic acid is correctly synthesized, the method comprising: synthesizing the first probe nucleic acid and one or more second probe nucleic acids on distinct regions of a substrate, wherein the first probe nucleic acid and the one or more second probe nucleic acids are synthesized on the substrate in the same manner, wherein the first probe nucleic acid has a length of n nucleotides (nt) and has a sequence complementary to a first target nucleic acid, and n ranges from about 10 nucleotides to about 100 nucleotides, wherein the one or more second probe nucleic acids have a sequence that is different from the sequence of the first probe nucleic acid and that is complementary to one or more second target nucleic acids, wherein the one or more second probe nucleic acids have a length ranging from 6 nucleotides to n nucleotides, wherein the first probe nucleic acid is synthesized and immobilized on the substrate in the same manner used to synthesize and immobilize the one or more second probe nucleic acids; hybridizing the one or more second target nucleic acids with the one or more second probe nucleic acids and forming a hybridized product, wherein the one or more second target nucleic acids are labeled with a detectable signal substance; determining a hybridized region of one of the second target nucleic acids on the hybridized product by measuring a signal generated from the detectable signal substance in said one of the second target nucleic acids on the hybridized product and comparing the signal with a reference signal, wherein the reference signal is obtained by hybridizing one of the second probe nucleic acids to one of the second target nucleic acids or hybridizing the first probe nucleic acid to the first target nucleic acid; and estimating whether the region of the first probe nucleic acid is correctly synthesized based on the results of hybridizing the one or more second target nucleic acids with the one or more second probe nucleic acids, wherein the region of the first probe nucleic acid corresponds to the hybridized region of said one of the second target nucleic acids.