Patent ID: 7125996

Claim:
A fluorescent probe having the following general formula (I): R 1 -L-X—Y (I) wherein R 1 is a nitropyrimidine of formula (III): wherein R 4 is selected from phenyl and naphthyl and is optionally substituted with one to three groups selected from Cl, F, Br, I, —CH 3 , —CN, —CO 2 CH 3 , —C(O)NR 5 R 6 , —NO 2 , —OH, —NH 2 , and —CF 3 ; L is a direct bond or a C 1 –C 16 alkylene group, a C 2 –C 16 alkenylene group or a C 2 –C 16 alkynylene group, wherein one or more of the available —CH 2 — groups present in the C 1 –C 16 alkylene group, C 2 –C 16 alkenlene group or C 2 –C 16 alkynylene group is optionally and independently replaced with —O—, —C(O)—, —S(O) p — wherein p is 0 to 2, or —N(R 2 )—; X is selected from the group consisting of O, S, —N(R 2 )C(O)—, —C(O)N(R 2 )—, —N(R 2 )C(S)—, —C(S)N(R 2 )—, —N(R 2 )C(S)NH—, —NHC(S)N(R 2 )—, —N(R 2 )C(O)NH—, —NHC(O)N(R 2 ), —SO 2 NR 2 —, —NR 2 SO 2 —, —CH 2 N(R 2 )—, —N(R 2 )CH 2 —, —CH 2 S—, —SCH 2 —, —C(O)CH 2 S—, SC(O)CH 2 —, —NHCH 2 CH 2 S—, —SCH 2 CH 2 NH—, —NC(O)O—, —ONC(O)—, —C(O)O—, —OC(O)—, —NH—N═C(R 2 )—, —C(R 2 )═N—NH—, —NHCH(R 2 )—, and —CH(R 2 )NH—; R 2 is H or C 1–3 alkyl; and Y is a fluorescent label which fluoresces at a wavelength above about 450 nm.