Patent ID: 8679757

Claim:
A method of analyzing at least one CGG-rich region comprised by at least one template in a sample, comprising: (a) providing at least two different primers, including a first primer comprising CGG, CCG, GGC, GCG, CGC, or GCC repeats, and a second primer that anneals to a position outside of the CGG-rich region; (b) performing PCR with the at least two different primers and the at least one template comprising the at least one CGG-rich region, wherein the PCR produces a set of products; (c) resolving the set of products with a high resolution technique to produce a representation of product size and abundance; and (d) deriving information about whether an interruptor sequence is present in the at least one CGG-rich region or where within the at least one CGG-rich region an interruptor sequence is located, wherein the method is an anchored assay.