Patent ID: 8507295

Claim:
A method of quantification of an analyte, A, in a test sample by means of a single lateral flow immunochromatographic device, comprising the steps of: a) mixing a determined amount of said test sample with a determined amount of a quantification agent, (QA 1 ), said QA 1 being capable of binding specifically and simultaneously both to an immobilized binding partner (BP) and to A, thus obtaining a mixture wherein the amount of A in said test sample is reflected by the ratio of the concentration of A-QA 1 complex formed to the concentration of free QA 1 in said mixture; b) applying a determined volume of said mixture to said device equipped with a test band in which said binding partner (BP) of the QA 1 is immobilized, so that A-QA 1 complex and free QA 1 are immobilized at said test band in a ratio that relates to their concentration ratio in the mixture applied, said volume of mixture being determined to provide a total amount of A-QA 1 complex and free QA 1 that saturates binding at the immobilized binding partner (BP) in said test band; c) measuring the amount of said A-QA 1 complex immobilized at the test band; and d) comparing the result obtained from the test sample with the results obtained from calibration samples containing known or allocated amounts of A and thus determining the amount of A in said test sample.