Patent ID: 8012708

Claim:
A method for measuring an environmental biological allergen(s), characterized in that said method comprises the steps of immersing a test sample collected by using an adhesive sheet in a solution containing a substrate of a protease which said allergen(s) has(have), in the state of being adhered to said adhesive sheet without a pretreatment, said substrate' being Boc-Val-Leu-Lys-3RAX and/or Boc-amino acid-3RAX (wherein in these formulae, Boc represents t-butyloxycarbonyl group which is a protective group of amino group, and 3RAX represents methylene violet 3RAX); and detecting the protease activity in said test sample using a color change of said substrate solution as an indicator of the presence and/or quantity of the environmental biological allergen(s), thereby detecting said biological allergen(s), wherein said allergen(s) to be measured are at least one selected from the group consisting of mite and materials originating from mite.