Patent ID: 7947817

Claim:
A composition comprising a nucleoside or a nucleotide comprising the formula: wherein R 1 is H, OH, a hydrophilic group, or a hydrophobic group; B is at least one homocyclic ring, at least one heterocyclic ring, at least one aryl group, or combinations thereof; BG is a blocking group which is a phosphate group —H 2 PO 4 Z is O or CH 2 ; and represents a single or double bond; wherein the nucleoside or nucleotide is labeled; and, further comprising one or more nucleotide incorporating biocatalysts selected from the group consisting of: G46E E678G CS5 DNA polymerase, a G46E L329A E678G CS5 DNA polymerase, G46E E678G CS6 DNA polymerase, Δ ZO5R polymerase, E615G Taq DNA polymerase, Thermus flavus polymerase, TMA-25 polymerase, TMA-30 polymerase, Tth DNA polymerase, Thermus specie SPS-17 polymerase, E615G Taq polymerase, Thermus ZO5R polymerase, T7 DNA polymerase, Kornberg DNA polymerase I, Klenow DNA polymerase, Taq DNA polymerase, Micrococcal DNA polymerase, alpha DNA polymerase, reverse transcriptase, AMV reverse transcriptase, M-MuLV reverse transcriptase, DNA polymerase, RNA polymerase, E. coli RNA polymerase, SP6 RNA polymerase, T3 RNA polymerase, T4 DNA polymerase, T7 RNA polymerase, RNA polymerase II, terminal transferase, polynucleotide phosphorylase, and ribonucleotide incorporating DNA polymerase, wherein the nucleoside or nucleotide is non-extendible by said one or more nucleotide incorporating biocatalysts.