Patent ID: 8188265

Claim:
A method for recovering formaldehyde-modified RNA from a formalin-fixed, paraffin-embedded biological tissue sample comprising: a) deparaffinizing the sample as known in the art, b) contacting said sample with a solution comprising an effective concentration of proteinase K and heating the sample in said solution to a temperature in the range of about 30 to about 60° C., for a time period of about 12 to 20 hours, c) adding an effective concentration of proteinase K to the solution obtained from b) for a time period of about 5 to about 30 minutes at a temperature range of about 40 to about 75° C., d) recovering said formaldehyde-modified RNA as known in the art, and e) purifying said formaldehyde-modified RNA, wherein said formaldehyde-modified RNA is purified by ethanol precipitation.