Patent ID: 8546106

Claim:
A method for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, wherein the fusion protein construct comprises: (i) a first polynucleotide comprising a nucleotide sequence encoding a signal peptide; (ii) a second polynucleotide comprising a nucleotide sequence encoding at least a catalytic domain of a Humicola Cel45 endoglucanase; and (iii) a third polynucleotide comprising a nucleotide sequence encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the Humicola Cel45 endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the Humicola Cel45 endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein comprising the amino acid sequence of SEQ ID NO: 74 or SEQ ID NO: 76 from the cultivation medium, wherein the fusion protein has biological activity.