Patent ID: 8241855

Claim:
A method for bisulfite treatment, wherein said method comprises: (a) providing a kit, said kit consisting of a reagent containing bisulfite, at least one denaturing solvent, at least one radical trap and primers for production of amplified DNA; (b) incubating a genomic DNA sample with a solution of the reagent containing bisulfite, wherein the bisulfite is in the concentration range between 0.1 and 6 mol/liter and in the presence of the at least one radical trap and the at least one denaturing solvent; (c) diluting the treated DNA sample with water or an aqueous solution; (d) amplifying the DNA sample in a polymerase reaction using the primers to yield amplified DNA; and (e) detecting how much the sequence has changed by the treatment according to step (b) in comparison to the genomic DNA sample and concluding the methylation state of at least one locus in the genomic DNA sample.