Patent ID: 8632994

Claim:
A method of detecting a biological marker in a sample comprising: a. contacting the sample with at least one first binding agent that specifically binds to the biological marker in the sample such that the at least one first binding agent binds to the sample and forms a first complex; b. contacting the first complex of a) with at least one second binding agent that specifically binds to the at least one first binding agent such that a second complex is formed, wherein the at least one second binding agent is linked to at least one first polymer; c. contacting the second complex of b) with at least one third binding agent that specifically binds to the at least one second binding agent, wherein the at least one third binding agent is linked to at least one second polymer; wherein at least one detectable agent is linked to at least one of i. the at least one third binding agent; ii. the at least one second polymer; or iii. both the at least one third binding agent and the at least one second polymer; wherein the at least one third binding agent binds to the second complex of b) and forms a third complex; and d. detecting the at least one detectable agent of the third complex of c), thereby detecting the biological marker in the sample.