Patent ID: 7829307

Claim:
A process for preparing a GLP-2 peptide, comprising: providing a single-chain protein multimer comprising at least two units of a GLP-2 peptide coupled tandemly by a linker consisting of the amino acid sequence ProValSerGlyProArg (SEQ ID NO: 12), wherein the single-chain protein multimer comprises an acid cleavage site between the Asp residue at the C-terminus of each internal GLP-2 unit and the N-terminal Pro of the linker, wherein the linker comprises a thrombin enzyme recognition sequence consisting of the amino acid sequence ValSerGlyProArg (SEQ ID NO: 11) abutting the N-terminus of each internal GLP-2 unit, wherein the single-chain protein multimer comprises a thrombin enzyme cleavage site between the Arg residue at the C-terminus of the linker and the first residue of the GLP-2 peptide at the N-terminus of each internal GLP-2 unit; cleaving the peptide bond at the acid cleavage site under conditions, wherein the pH is adjusted to a pH from about 1 to about 3 cleaving the peptide bond at the thrombin enzyme cleavage site with a thrombin enzyme; thereby liberating said at least two GLP-2 peptide units, wherein each GLP-2 peptide unit has an authentic N- and C-terminal residue; and isolating the resulting GLP-2 peptide units.