Patent ID: 8632969

Claim:
A method for detecting a nucleic acid, comprising: providing a field-effect transistor (FET) based biomolecule detection device comprising: a source region and a drain region disposed apart from each other on a semiconductor substrate, a gate electrode layer formed between the source region and the drain region, a gate adsorption layer formed on an upper surface of the gate electrode layer, wherein the gate adsorption layer comprises a polymer having a positive (+) charge or an inorganic material, a chamber formed over the semiconductor substrate including the gate electrode layer, wherein the chamber can be used as a space for accommodating the nucleic acid-containing sample; injecting a sample containing an adsorption medium with a nucleic acid adsorbed thereon into the chamber of the biomolecule detection device; washing the sample in the chamber to remove impurities; heating the sample in the chamber to denature the nucleic acid, wherein the heat is generated by a heating means within the semiconductor substrate; adsorbing the denatured nucleic acid to the gate adsorption layer of the biomolecule detection device; cooling down the sample; and detecting a nucleic acid in the sample by using the biomolecule detection device.