Patent ID: 7175750

Claim:
A temperature gradient electrophoresis-based method for generating data indicative of the presence of a single nucleotide polymorphism or a mutation in a biological sample, comprising: providing an unknown double-stranded polynucleotide sample; subjecting the unknown double-stranded polynucleotide sample to temperature gradient gel electrophoresis, the unknown double-stranded polynucleotide sample including non-desalted polymerase chain reaction (PCR) products; subjecting a reference double-stranded polynucleotide to temperature gradient electrophoresis; irradiating the unknown double-stranded polynucleotide with light to generate a first spectroscopic signal, irradiating the reference double-stranded polynucleotide with light to generate a second spectroscopic signal; converting the first and second spectroscopic signals into first and second data; and determining the presence of a single nucleotide polymorphism or a mutation in the unknown double-stranded polynucleotide sample based on the first and second data.