Patent ID: 7745174

Claim:
A method for co-expressing two heterotypes of Hepatocyte Growth Factor (HGF) comprising transforming or transfecting to a cell a DNA construct comprising: (a) a promoter, (b) a first cDNA which has the same sequence as exons 1-4 of the human HGF gene wherein said exons 1-4 are arranged in sequential order without an intron therebetween, or degenerates thereof which do not alter the amino acid sequence encoded by said first cDNA, (c) a polynucleotide that has the same sequence as intron 4 of the HGF gene or a functional fragment thereof, and (d) a second cDNA which has the same sequence as exons 5-18 of the human HGF gene wherein said exons 5-18 are arranged in sequential order without an intron therebetween, or degenerates thereof which do not alter the amino acid sequence encoded by said second cDNA; wherein (c) is located between (b) and (d); and the HGF construct simultaneously encodes two heterotypes of human HGF.