Patent ID: 8715934

Claim:
A method for analyzing nucleotide sequence variations, comprising: forming microemulsions comprising an oil phase and an aqueous phase, wherein the aqueous phase comprises one or more species of analyte DNA molecules, wherein the aqueous phase comprises from 10-30% (v/v) of the microemulsions and the oil phase comprises from 70-90% (v/v) of the microemulsions; wherein the oil phase comprises one or more low viscosity hydrocarbons with a viscosity less than 20 mPas at 25° C. in an amount from 60-85% (v/v) of the oil phase, one or more high viscosity hydrocarbons having a viscosity of greater than 20 mPas at 25° C. in an amount from 10-30% (v/v), and an emulsifier in an amount from 5-10% (v/v); amplifying analyte DNA molecules in the microemulsions in the presence of reagent beads, wherein the reagent beads are bound to a plurality of molecules of a primer for amplifying the analyte DNA molecules, whereby product beads are formed which are bound to a plurality of copies of one species of analyte DNA molecule; separating the product beads from analyte DNA molecules which are not bound to product beads; determining a sequence feature of the one species of analyte DNA molecule which is bound to the product beads.