Patent ID: 8603785

Claim:
A method of severing an ether bond in octylphenol polyethoxylates (OPEOn) by using dihydrolipoamide dehydrogenase (DLD), comprising the steps of: (a) obtaining a gene of DLD from a strain of Pseudomonas nitroreducens TX1; (b) recombining said gene of DLD by inserting said gene of DLD into a vector for expression as a tag fusion protein in Escherichia coli to obtain a recombinant DLD encoded by the sequence of SEQ ID NO:1; (c) processing an isolation step and a purification step of said recombinant DLD through column chromatography by affinity and gel filtration to result in a purified DLD; and (d) adding said purified DLD to a solution of octylphenol polyethoxylates (OPEOn), allowing said severing of said ether bond.