Patent ID: 7045338

Claim:
A mutant replication region which is at least 95% identical to the a nucleotide sequence as set forth in SEQ ID NO:1, and which has at least one point mutation independently selected from the group consisting of: a) a mutation of T to A at nucleotide number 72, b) a mutation of T to C at nucleotide number 412, c) a mutation of A to G at nucleotide number 538, d) a mutation of A to T at nucleotide number 1012, e) a mutation by deletion of C at nucleotide number 1069, f) a mutation of C to G at nucleotide number 1094, g) a mutation of G to C at nucleotide number 1155, h) a mutation of T to C at nucleotide number 1220, i) a mutation of T to C at nucleotide number 1243, j) a mutation by deletion of C at nucleotide number 1271, and k) a mutation of T to G at nucleotide number 1336, wherein said mutant replication control region conveys temperature sensitivity to a plasmid.