Patent ID: 8642293

Claim:
A disposable device capable of holding and processing samples for being operated in a nucleic acid amplification apparatus, comprising: a rigid body comprising a top surface and a bottom surface oriented in a substantially vertical plane, a top surface, and a bottom surface, a binding chamber comprising a first cavity recessed into the top surface of said rigid body and containing a solid phase for solid phase extraction of a component of a sample to be analyzed, an amplification chamber comprising a second cavity recessed into the top surface of said rigid body, a sample preparation chamber comprising an elongated concave cavity recessed into the top surface of said rigid body and comprising a cylindrical opening situated below the vertical plane of the rigid body, the cylindrical opening configured to receive a sample transfer tip, the sample preparation chamber further comprising a venting system having a fluidic connection between the sample preparation chamber and the ambient allowing gas exchange, at least a first fluid channel leading from the binding chamber to an inlet port, the first fluid channel comprising a first sealing point configured to deform in its width from an open position to a closed position sealing together opposite walls of the first fluid channel interrupting flow of liquid or gas through said first fluid channel, at least a second fluid channel connecting said amplification chamber to said binding chamber, the second fluid channel comprising a second sealing point configured to deform in its width from an open position to a closed position sealing together opposite walls of the second fluid channel interrupting flow of liquid or gas through said second fluid channel, and at least one fluid interface connected by another fluid channel to at least one of the binding chamber and the amplification chamber, wherein the fluid interfaces are situated on at least one of the top and bottom-surfaces of the rigid body when the device is operated in the nucleic acid amplification apparatus, wherein: each of said surfaces, on which a channel, the binding chamber or the amplification chamber is situated, is covered by at least one wall, and the surfaces are oriented along substantially vertical planes when the device is operated in the nucleic acid amplification apparatus.