Patent ID: 8883422

Claim:
A method for identifying the presence or absence of a lactic acid bacterial strain comprising a uniquely located insertion sequence (IS) element in a dairy product, the method comprising: (a) obtaining a nucleic sample from a dairy product, (b) providing a primer pair specific for a region of said uniquely located IS element and a region of a nucleic acid sequence adjacent to said uniquely located IS element, the primer pair selected from the group consisting of: (i) forward primer comprising SEQ ID NO: 1 and reverse primer comprising SEQ ID NO: 3; (ii) forward primer comprising SEQ ID NO: 1 and reverse primer comprising SEQ ID NO: 2; (iii) forward primer comprising SEQ ID NO: 4 and reverse primer comprising SEQ ID NO: 5; or (iv) forward primer comprising SEQ ID NO: 6 and reverse primer comprising SEQ ID NO: 7, wherein at least one of the primers of said primer pair comprises an attached fluorescent dye; (c) performing a PCR amplification reaction with said primer pair of step (b) under conditions suitable to produce an amplification product when said uniquely located IS element is present in said nucleic acid sample, and (d) identifying the presence or absence of a lactic acid bacterial strain by detecting the presence or absence of said amplification product, wherein said lactic acid bacterial strain is the Pediococcus acidilactii strain deposited on Sep. 25, 2009 with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) under Accession No. DSM 22981 or the Lactobacillus delbrueckii subsp. lactis strain deposited on Sep. 28, 2010 with the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) under Accession No. DSM 24025, or a combination thereof.