Patent ID: 8349906

Claim:
A method of separating or purifying a positively charged material, the method comprising: preparing a macroporous cation exchange resin, the preparing comprising forming an aqueous phase composition comprising a) a monomer mixture comprising i) a crosslinking monomer comprising N,N′-alkylenebis(meth)acrylamide, N,N′-heteroalkylenebis(meth)acrylamide, or a combination thereof in an amount in a range of 15 to 90 weight percent based on a weight of monomers in the monomer mixture; and ii) a negatively charged ionic monomer; and b) an aliphatic, water-soluble porogen having at least three hydroxy groups; and c) an aqueous phase solvent mixture comprising water and a mono-alcohol having 1 to 4 carbon atoms, wherein the mono-alcohol is present in an amount of at least 20 weight percent based on a total weight of the aqueous phase solvent mixture; suspending the aqueous phase composition in a non-polar organic solvent; polymerizing the monomer mixture to form particles of polymeric material; removing the porogen from the particles to form the macroporous cation exchange resin having a capacity greater than 30 mg/mL for Immunoglobulin G; contacting the macroporous cation exchange resin with positively charged material; and adsorbing at least a portion of the positively charged material on the macroporous cation exchange resin.