Patent ID: 8445251

Claim:
A recombinant single-chain meganuclease comprising: (a) a first subunit comprising a polypeptide having at least 85% sequence identity to residues 9-151 of SEQ ID NO: 1; (b) a second subunit comprising a polypeptide having at least 85% sequence identity to residues 9-151 of SEQ ID NO: 1; and (c) a polypeptide linker, said polypeptide linker being covalently bound to said first and said second subunit; said first subunit being covalently bound to said polypeptide linker at a residue corresponding to a position selected from the group of positions 153-163 of SEQ ID NO: 1; said second subunit being covalently bound to said polypeptide linker at a residue corresponding to a position selected from the group of positions 1-9 of SEQ ID NO: 1; wherein said polypeptide linker is SLPGSVGGLSPSQASSAASSASSSPGSGISEALRAGATKS (SEQ ID NO: 77); wherein said first and second subunits are capable of functioning together to recognize and cleave a non-palindromic DNA sequence.