Patent ID: 7314928

Claim:
Process for manufacturing a morpholino-nucleotide of formula (I): in which R 1 represents a nucleic base and R 2 is selected from the group consisting of: —(CH 2 ) n —NH—R 3 —(CH 2 ) n —SR 3 —(CH 2 ) n —CO—R 3 and —(CH 2 ) n —OR 3 in which n is an integer ranging from 1 to 12 and R 3 is selected from the group consisting of a label, a protein, an enzyme, a fatty acid, and a peptide, said process comprising a) reacting a nucleoside triphosphate of formula (II): with a periodate, a compound of formula R 2′ NH 2 , wherein R 2′ is selected from the group consisting of: —(CH 2 ) n —NH 2 —(CH 2 ) n —SH —(CH 2 ) n —COOH and —(CH 2 ) n —OH, and sodium borohydride to form a morpholino-nucleotide of formula (III): b) isolating the morpholino-nucleotide of formula (III); and c) attaching R 3 to the morpholino-nucleotide of formula (III) to form the morpholino-nucleotide of formula (I).