Patent ID: 8765403

Claim:
A method of producing a product or a product precursor of a biosynthetic pathway in a genetically modified host cell, the method comprising culturing a genetically modified host cell under suitable conditions, wherein the genetically modified host cell is genetically modified with one or more nucleic acids comprising: a) a nucleotide sequence encoding a scaffold polypeptide of the formula (X 1 ) n1 (Y 1 )(X 2 ) n2 (Y 2 )(X 3 ) n3 , wherein X 1 , X 2 , and X 3 are different peptide binding elements, wherein each of n 1 , n 2 , and n 3 is independently an integer from one to about 10, and wherein Y 1 and Y 2 , if present, are linker peptides, wherein the ratio of n 2 to n 3 provides for at least 25% increased production of the product or product precursor compared to the level of the product or product precursor produced in the corresponding control cell not genetically modified with a nucleic acid comprising a nucleotide sequence encoding the scaffold polypeptide; and b) nucleotide sequences encoding at least a first and a second enzyme in a biosynthetic pathway, wherein the first enzyme comprises a first heterologous peptide that binds a first peptide binding element in the scaffold polypeptide, wherein the second enzyme comprises a second heterologous peptide that binds a second peptide binding element, and wherein the first enzyme produces an intermediate that is a substrate for the second enzyme, said culturing providing for synthesis of said scaffold polypeptide and said enzymes in the genetically modified host cell, resulting in production of said product or product precursor.