Patent ID: 7781173

Claim:
A method for estimating or determining an apparent or actual molecular weight of an uncharacterized molecule using a molecular weight standard set comprising at least two protein standards of known and different molecular weights, the method comprising: a) separating by electrophoresis the uncharacterized molecule and at least one protein standard of the molecular weight standard set; b) detecting the separated uncharacterized molecule and at least one separated protein standard of the molecular standard set; and c) comparing at least one separated protein standard of the molecular standard set to the separated uncharacterized molecule to estimate or determine the apparent or actual molecular weight of the uncharacterized molecule; wherein at least one of the at least two protein standards is a high molecular weight protein standard having an apparent molecular weight of at least 250 kDa by SDS PAGE, wherein the high molecular weight protein standard is at least a partially homogeneous population of an isolated laminin polypeptide, wherein at least one post-translational modification has been removed from the isolated laminin polypeptide to produce the at least partially homogenous population of the isolated laminin polypeptide, and wherein the post-translational modification is selected from the group consisting of glycosylation and phosphorylation.