Patent ID: 7682839

Claim:
A method for detecting a labeled compound in a sample, wherein the labeled compound comprises a compound linked to a chemiluminescent label, said labeled compound having the formula: wherein each of R 1 , R 2 and R 3 is selected from substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted aryl, substituted or unsubstituted aralkyl groups of 1-20 carbon atoms, wherein R 1 and R 2 can be joined together in a ring, R 4 -R 11 are independently selected from hydrogen and substituents which do not interfere with the generation of chemiluminescence, and at least one of R 1 -R 11 is linked to the compound by means of L-RG, wherein L is a linking group and RG is a reactive group, said method comprising: a) reacting the labeled compound with a peroxide and a peroxidase for producing chemiluminescence from the chemiluminescent label wherein the ratio of labeled compound to peroxidase ranges from 100:1 to about 1:1000 and the peroxide is added in a concentration in the range of 10 −6 M to 10 −1 M to produce the chemiluminescence as a flash of light lasting not more than about five seconds; and b) detecting the chemiluminescence as an indication of the presence of said compound in said sample.