Patent ID: 7384738

Claim:
A method for analyzing genomic DNA clones comprising: a) providing at least one vector and at least one packaging cell, wherein said at least one vector comprises retroviral 5′ and 3′ long terminal repeats flanking a genomic DNA sequence, wherein said genomic DNA sequence is from 20 kilobases to 200 kilobases in length and includes introns; b) introducing said at least one vector into said at least one packaging cell under conditions such that a RNA molecule corresponding to said retroviral 5′ and 3′ long terminal repeats and said genomic DNA with said introns removed sequence is transcribed and said RNA molecule is packaged into said retroviral particle, wherein said introns are removed from said RNA prior to said packaging, and wherein after removal of said introns, said RNA is 11 kilobases or less in size; c) transducing a cell line having a genome with said retroviral particle under conditions such that said RNA molecule corresponding to said retroviral 5′ and 3′ long terminal repeats flanking a genomic DNA sequence with said introns removed is reverse transcribed and inserted into the genome of said cell line as a provirus; and d) analyzing said genomic clone in said provirus.