Patent ID: 8173377

Claim:
A method of using an oligonucleotide array and compensating for insufficient deprotection or insufficient elongation of oligonucleotides on said array, comprising the steps of: (a) providing a substrate having a plurality of different oligonucleotides immobilized thereon, with said different oligonucleotides immobilized in different separate and discrete locations on said substrate; (b) providing indicia associated with said array, said indicia recording the presence of insufficient deprotection or insufficient elongation of at least one oligonucleotide, said at least one oligonucleotide located in a separate and discrete locations on said array; (c) providing a test compound; (d) detecting the binding of said test compound to at least one of said plurality of different oligonucleotides; and then (d) determining the degree of binding of said test compound to said oligonucleotide from (i) said detected binding and (ii) said indicia recording the presence of insufficient deprotection or insufficient elongation, so that said insufficient deprotection or insufficient elongation is compensated for during said determining step.