Patent ID: 7541186

Claim:
A method for in vitro differentiation of human retinal progenitor cells from human embryonic stem cells, the method comprising: culturing said human embryonic stem cells to embryoid bodies in a conditioning medium comprising at least 1 ng/ml of insulin-like growth factor 1 (IGF-1), at least 1 ng/ml Dkk-1 and at least 1 ng/ml Noggin for 1 to 7 days in the absence of basic fibroblast growth factor (FGF2) activity; then culturing the resulting embryoid bodies in retinal differentiation medium comprising at least 1 ng/ml of insulin-like growth factor 1 (IGF-1), at least 1 ng/ml Dkk-1, at least 1 ng/ml Noggin and at least 5 ng/ml FGF-2 for at least one week, thereby yielding human retinal progenitor cells.