Patent ID: 8791086

Claim:
A pharmaceutical composition comprising, a polyion complex comprising a polyanionic substance as an active ingredient that suppresses the expression of PHD2, a polycation chargeable polymer, and glycosaminoglycan, which forms a ternary system complex of the polyion complex and glycosaminoglycan, wherein the polyanionic substance is selected from the group consisting of RNAi inducing nucleic acid against PHD2 or an expression vector thereof, antisense nucleic acid against PHD2 or an expression vector thereof, and a ribozyme against PHD2 or an expression vector thereof, and the polycation chargeable polymer is a chargeable polymer represented by the following formula (III), a block copolymer represented by the following formula (I) or (II), or a salt thereof, wherein, R 10 is a hydroxy group, an oxybenzyl group or an NH—R 11 group, wherein R 11 is an optionally substituted straight chain or branched C 1-20 alkyl group, R 1a and R 1b are each independently a hydrogen atom or an optionally substituted straight chain or branched C 1-12 alkyl group, R 2a , R 2b , R 2c and R 2d are each independently a methylene group or an ethylene group, R 3 is a hydrogen atom, a protecting group, a hydrophobic group or a polymerizable group, R 4 is a hydroxy group, a protecting group or a group represented by —O—X 3 , —S—X 3 , —NH—X 3 , or a polymerization initiator residue of polypeptide, wherein X 3 is a primary, secondary or tertiary amine compound, or an amine compound residue comprising one or more groups derived from a quaternary ammonium salt, or a non-amine compound residue, R 5a , R 5b , R 5c and R 5d are each independently a hydroxy group, an oxybenzyl group or an NH—(CH 2 ) a —X group, wherein a is an integer of 1-5, X is each independently a primary, secondary or tertiary amine compound, or an amine compound residue comprising one or more groups derived from a quaternary ammonium salt, or a non-amine compound residue, the total of R 5a and R 5b or R 5c and R 5d contains at least two —NH—(CH 2 ) a —X 1 groups (wherein X 1 is (NH(CH 2 ) 2 ) 2 ) e —NH 2 , and e is an integer of 1-5), R 6a and R 6b are each independently a hydrogen atom or protecting group, wherein the protecting group is selected from the group consisting of a Z group, a Boc group, an acetyl group and a trifluoroacetyl group, L 1 and L 2 are linking groups, m is an integer of 5-20,000, n is an integer of 2-5,000, y is an integer of 0-5,000, z is an integer of 0-5,000, and y+z is not larger than n, and each repeat unit in the above formulas may be present in the above specified order or may be present in a random order.