Patent ID: 8809519

Claim:
A method for isolating nucleic acids comprising: contacting a sample containing nucleic acids with a solid phase in a first aqueous solution having a pH from about 6 to about 9 to provide a loaded solid phase; wherein the first aqueous solution comprises one or more salts; and the surface of the solid phase comprises one or more functional groups having structural formula (I): wherein, X is NR 1 , S, or O; Y is —OH, —SH, —NHR 2 , —B(OH) 2 , or —B(OR 3 ) 2 ; R 1 is hydrogen or alkyl; R 2 is hydrogen, alkyl, or aryl; each R 3 is independently alkyl or aryl, or alternatively, two of R 3 together with the atoms to which they are bonded from a heterocyclyl; and represents the point of attachment to the solid phase; washing the loaded solid phase with a second aqueous solution having a pH from about 6 to about 9 to provide a washed solid phase; wherein the second aqueous solution comprises a detergent and one or more salts; and isolating the nucleic acids by eluting the washed solid phase with a low ionic strength liquid having a pH from about 5 to about 9 to obtain the isolated nucleic acids.