Patent ID: 8399246

Claim:
A baculovirus expression vector, comprising: a first gene cassette configured for displaying a first influenza surface protein onto an envelope of the baculovirus, including a first influenza surface protein sequence encoding the first influenza surface protein; a baculovirus protein signal sequence operably linked to a 5′ end of the first influenza surface protein sequence and encoding a baculovirus signal peptide, wherein the baculovirus signal peptide comprises a membrane localization sequence linked to an N-terminal domain of the first influenza surface protein; a baculovirus cytoplasmic domain sequence operably linked to a 3′ end of the first influenza surface protein sequence and encoding a baculovirus cytoplasmic domain peptide comprising a membrane anchoring sequence; and a first promoter operably linked to the first influenza surface protein sequence and the baculovirus protein signal sequence, wherein the first promoter is a baculovirus promoter; and a second gene cassette configured for endogenously expressing a second influenza surface protein and generating immunogenicity in a host comprising a mammalian or an avian, including a second influenza surface protein sequence encoding the second influenza surface protein, wherein the first influenza surface comprises a hemagglutinin or a neuraminidase, and the first influenza surface protein and the second influenza surface protein are the same; and a second promoter operably linked to the second influenza surface protein sequence, wherein the second promoter is a promoter for the mammalian or the avian.