Patent ID: 7947468

Claim:
A method for identifying a compound expected to be useful in inhibiting LRRK2 protein kinase activity, the method comprising the steps of: (1) determining whether a test compound inhibits the protein kinase activity of a LRRK2 polypeptide on a substrate polypeptide, and (2) selecting a compound which inhibits said LRRK2 polypeptide protein kinase activity, wherein the substrate polypeptide comprises a sequence selected from the group consisting of RLGRDKYKTLRQIRQ (SEQ ID NO:1), RLGRDKYKSLRQIRQ (SEQ ID NO:3), WWRFYTLRRA (SEQ ID NO:65), WWKFYTLRRA (SEQ ID NO:47), WWRFYTLRKA (SEQ ID NO:48), MRQGRDKYKTLRQIRQGNTKQRIDEFEAL (SEQ ID NO:44), VKAGRDKYKTLRQIRQGNTKQRIDEFEAM (SEQ ID NO:45), RLGRDKYK(T/S)LRQIRQGNTKQR (SEQ ID NO:4), RLGWWRFYTLRRARQGNTKQR (SEQ ID NO:51), RLGWWKFYTLRRARQGNTKQR (SEQ ID NO:49), RLGWWRFYTLRKARQGNTKQR (SEQ ID NO:50), moesin (SEQ ID NO:39), ezrin (SEQ ID NO:70), radixin (SEQ ID NO:41), or a fusion peptide thereof.