Patent ID: 8772008

Claim:
A method for increasing the nuclease activity of at least one zinc finger nuclease in an isolated mammalian cell, the method comprising: (a) culturing the isolated mammalian cell at an optimal growth temperature above 33° C.; (b) transfecting the isolated mammalian cell with a polynucleotide encoding the at least one zinc finger nuclease (ZFN) into the isolated mammalian cell from step (a); (c) culturing the isolated mammalian cell from step (b) at a temperature between 27° C. and 33° C. for between 1 and 4 days; and (d) culturing the isolated mammalian cell following step (c) at the optimal growth temperature, such that the nuclease activity of the ZFN is increased as compared to an isolated mammalian cell transfected and cultured only at the optimal growth temperature without the culturing step (c).