Patent ID: 7348172

Claim:
A method for increasing the production level of Nonomuraea flexuosa xylanase Xyn11A (Nf Xyn11A) in a filamentous fungal host, said method comprising: expressing a DNA construct comprising a DNA sequence comprising DNA encoding the amino acid sequence of a truncated form of the Nf Xyn11A mature protein in a filamentous fungal host, said amino acid sequence of said truncated form of said mature protein consisting of the amino acid sequence of SEQ ID NO: 12, and secreting said truncated form of said Nf Xyn11A, wherein said production level obtained using said DNA construct, when measured as an increase of activity from the culture media of single copy transformants, is higher than the production level obtained using a corresponding DNA construct that is identical to said DNA construct encoding said truncated form, except that it comprises a DNA sequence encoding the sequence of the full length Nf Xyn11A xylanase (SEQ ID NO: 11) instead of a DNA sequence encoding said truncated form.