Patent ID: 7560264

Claim:
A method for promoting auto-induction of transcription of cloned DNA in bacterial cells grown batchwise, which cells are selected from the group consisting of BL21(DE3), B834(DE3), HMS174(DE3) and derivatives thereof, said transcription being under the control of a lac or lacUV5 promoter, the method comprising: a) providing a culture medium selected from the group consisting of ZYP-5052, PA-5052, P-5052, PASM-5052, MAS-15052, MS-15052, ZYM-15052 and combinations thereof; b) inoculating said culture medium with an inoculum of a said bacterial cells; and c) incubating the inoculated culture medium of step b) under conditions for growth of said cells until a saturating cell density has been achieved.