Patent ID: 7307057

Claim:
Antibiotic 107891 complex, isolated from Microbispora sp. ATCC PTA-5024, comprising Factor A1 and Factor A2 being a white powder having the following characteristics: (A) Mass spectrum recorded from a 0.2 mg/nil solution in methanol:water 80/20 (v/v) with trifluoracetic acid 0.1% on a Thermofinnigan LCQ deca instrument fitted with an electrospray source, using Thermofinnigan calibration mix under the following electrospray conditions: spray voltage: 4.7 kV; capillary temperature: 220.degree. C.; capillary voltage: 3V; infusion mode 10.mu.1/min, showing two double protonated ions at m/z 1124 and nih 1116, corresponding to the lowest isotope composition of Factor A1 and A2, respectively; (B) Infrared spectrum recorded in KBr with a Bruker FT-IR spectophotometer model IFS 48, exhibiting absorption maxima at (cm −1 ) 3263; 2929; 1661; 1533; 1402; 1114; 1026; C) U.V. spectrum perfommed in methanol:H 2080:20 (v/v) with a Perkin-Elmer spectrophotometer Lambda 16, exhibiting two shoulders at 226 and 267 nm; D) 1 H-NMR spectrum recorded at 600 MHz in the mixture methanol-d4:H 2 O (pH 4.3 HCl) 40:10 (v/v) at 40 degrees C. on a Bruker AMX 600 spectrometer applying a water suppression sequence using as internal standard the residual signal of methanol-d4 at 3.31 ppm, exhibiting the following signals [δ=ppm muliplicity; (attribution)]:0.93 d (CH 3 ), 0.98 d (CH 3 ), 1.07 t (overlapped CH 3 's), 1.18 t (overlapped CH 3 's), 1.26 s (CH 3 ), 1.30 t (overlapped CH 3 's), 1.62-1.74 m (CH 2 ), 1.78 d (CH 3 ), 1.80 d (CH 3 ), 2.03 m (CH 2 ), 2.24 m (CH), 2.36 m (CH 2 ), 2.72-3.8 m (peptidic alpha CH's), 3.8-5.2 m (peptidic alpha CH's), 5.53-6.08 s (CH 2 ), 5.62 d (CH double bond), 6.42 m (CH), 6.92 d (CH double bond), 7.0-7.55 m (aromatic CH's), 7.62-10.4 d and m (aromatic and peptidic NH's); E) 13 C-NMR spectrum recorded in the mixture methanol-d4:H2O (pH 4.3 HCl) 40:10 (v/v) at 40 degrees C. on a Bruker AMX 600 spectrometer, using as internal standard the residual signal of methanol-d4 at 49.15 ppm, exhibiting the following signals: [δ=ppm; (attribution)]: 13.6-23.2 (aliphatic CH 3 's), 26.16-73 (aliphatic CH 2 's and peptidic alpha CH's), 105-136 (aromatic and double bonds CH's and quartenary carbons), 164.3-176.3 (peptidic carbonyls); F) the acid hydrolysate in 6N HCl, (105 degrees C., 24 h) showing the presence of the following amino acids, along with other unidentified peaks, after derivatization with 6-aminoquinolyl-N-hydroxysuccinmmidyl carbamate: lanthionine, methyllanthionine, glycine, proline, valine, aspartic acid (hydrolysis product of asparagine), phenylalanine and leucine; G) the acid hydrolysate in 4N methanesulfonic acid containing 0.2% (w/v) 3-(2-aminoethyl) indole as catalyst (115 degrees C., 16 h) showing the presence of 5-chlorotryptophan; and H) a basic ionizable function detected by acid/base titration performed with 0.01 N potassium hydroxide in 2-methoxyethanol (MCS):H 2 O 12:3 (v/v) containing a molar excess of 0.01 N hydrochloric acid.