Patent ID: 8546307

Claim:
A method of screening a phagemid library of proteins of interest, wherein the library comprises a plurality of vectors which each comprise a polynucleotide coding for a protein of interest linked to a polynucleotide coding for a triple tag sequence, wherein the triple tag sequence comprises 6X histidine (SEQ ID NO: 1), c-myc (SEQ ID NO: 2) and V5 (SEQ ID NO: 3), said method comprising: (a.) expressing a plurality of proteins of interest linked to the triple tag sequence from the plurality of vectors; (b.) screening the proteins of interest linked to the triple tag sequence for binding to a binding partner of the protein of interest; and (c.) recovering the proteins of interest linked to the triple tag sequence that are bound in step (b.) by capture with an antibody specific for V5, c-myc or 6X histidine, wherein the triple tag sequence is HHHHHHGAAEQKLISEEDLKAAGKPIPNPLLGLDST (SEQ ID NO: 5), and wherein the protein of interest is a single chain antibody.