Patent ID: 7582459

Claim:
A method for recovering a polysaccharide from a fermentation broth, comprising: employing a cationic detergent to precipitate the polysaccharide or part of the contaminants from the supernatant to obtain a first polysaccharide fraction; employing alcohol to precipitate the polysaccharide from the first polysaccharide fraction to obtain a second polysaccharide fraction; subjecting the second polysaccharide fraction to an alcohol precipitation in the presence of an anionic detergent, whereby the alcohol is present in a concentration which is below a concentration at which the polysaccharide precipitates; precipitating the polysaccharide from the soluble fraction employing alcohol to obtain a polysaccharide precipitate; and dissolving the polysaccharide precipitate and subjecting it to concentration and diafiltration.