Patent ID: 7138116

Claim:
A humanized immunoglobulin which binds to human von Willebrand factor, comprising (a) complementarity determining regions comprising amino acid sequences RFWMS, residues 49–53 of SEQ ID NO: 6, EVNPDNNTMNYTPSLKD, residues 68–84 of SEQ ID NO: 6, and PPYYGSYGGFAY residues 117–128 of SEQ ID NO: 6, in the heavy chain (HC), and RASENIYNNLA, residues 44–54 of SEQ ID NO: 8, AATNLAD, 70–76 of SEQ ID NO: 8, and QHLWTSPYT, residues 109–117 of SEQ ID NO: 8, in the light chain (LC), and (b) framework regions of human antibody, wherein the framework regions of a human antibody correspond to residues 21–43, 55–69, 77–108, and 118–127 of SEQ ID NO: 8 for the light chain and to residues 19–48, 54–67, 85–116, and 119–139 of SEQ ID NO: 6 for the heavy chain, and wherein at least one amino acid selected from the group consisting of LC-48, residue 68 of SEQ ID NO: 8, LC-70, residue 90 of SEQ ID NO: 8, LC-71, residue 91 of SEQ ID NO: 8, HC-28, residue 46 of SEQ ID NO: 6, HC-48, residue 66 of SEQ ID NO: 6, HC-49, residue 67 of SEQ ID NO: 6, and HC-67, residue 85 of SEQ ID NO: 6, is replaced with an amino acid present in the equivalent position of a mouse AJvW-2 antibody heavy or light chain variable region framework.