Patent ID: 8574860

Claim:
An assay comprising: a) measuring or quantifying the polypeptide level of at least one biomarker selected from mucin 13 (MUC13), bile salt-activated lipase (CEL), dipeptidyl peptidase 4 (DPP4) and carboxypeptidase A1 (CPA1) polypeptides in a test blood sample or fraction thereof and/or amniotic fluid sample from a pregnant subject; and b) comparing the measured or quantified level of the at least one biomarker with a corresponding reference biomarker polypeptide level, and if the level of MUC13, CEL, DPP4, and/or compared to the reference biomarker polypeptide level, identifying the subject as having an increased probability of trisomy 21 in the fetus.