Patent ID: 8591942

Claim:
A method of preparing liposomes containing docetaxel, the method consisting of: (a) dispersing soy phosphatidylcholine and sodium oleate in an aqueous medium to produce dispersed liposomes, wherein the aqueous medium is free of protein; (b) adding a disaccharide to the dispersed liposomes; (c) homogenizing the liposomes of step (b) with a microfluidic homogenizer at a pressure of about 15,000 psi; (d) homogenizing solid docetaxel with the homogenized liposomes of step (c) with a microfluidic homogenizer at a pressure of about 15,000 psi to form liposomes containing docetaxel at a concentration of about 5-20 mg/ml; and (d) optionally lyophilizing the liposomes containing the docetaxel, wherein the method is performed at room temperature and without organic solvents.