Patent ID: 8778693

Claim:
Method for the identification, characterization and quantification by a single detection of carboxylic functional groups, comprising: a. in a first step, a sample previously taken for analysis is mixed with a solution for dissolving said sample to be tested, previously prepared by mixing at least one buffer, a primary activating agent of the carbons bearing the carboxylic functional groups and a secondary activating agent, and this mixture is incubated for a given time; b. in a second step, a fluorescent primary amine dissolved in ultrapure water or in a buffer is added to the mixture obtained in the first step; c. in a third step, a compound chosen from the members of the dialdehyde family, previously dissolved in an aqueous buffer of basic pH or in an organic solvent, or in a mixed (organic solvent/water) solvent, is added to the mixture obtained in the second step; d. in a fourth step, an induced fluorescence of the mixture obtained in the third step is measured at a wavelength comprised between 390 and 490 nm after excitation at a wavelength comprised between 300 to 350 nm; e. in a fifth step, a value of the fluorescence obtained in the fourth step is referred to a calibration curve previously established under the same conditions.