Patent ID: 8206950

Claim:
A method for making a vaccinal virus fusion antigen, comprising: selecting a segment of a virus protein sequence that comprises at least one epitope; synthesizing a DNA fragment encoding the selected segment of the virus protein sequence; inserting the DNA fragment encoding the selected segment of the virus protein sequence into a Pseudomonas Exotoxin A (PE) vector to obtain a fusion gene plasmid, in which the PE vector comprises: (i) a PE fragment comprising a binding domain and a translocation domain and is without a cytotoxic domain; and (ii) a carboxyl terminal moiety comprising an endoplasmic reticulum retention sequence, wherein the DNA fragment encoding the selected segment of the virus protein sequence is inserted between the PE fragment and the carboxyl terminal moiety, thereby generating a fusion gene plasmid; and expressing the fusion gene plasmid in a host cell to obtain the vaccinal virus fusion antigen, wherein the vaccinal virus fusion antigen comprises: (i) the PE fragment; (ii) the selected segment of the virus protein sequence; and (iii) the carboxyl terminal moiety comprising the endoplasmic reticulum retention sequence.