Patent ID: 7300802

Claim:
A membrane strip biosensor system comprising: (a) a first group of membrane pads comprising: (i) a first membrane pad for sample application, (ii) a second membrane pad for release of a detection binding component, said second membrane pad comprises a tracer-linked binding component specific for and able to bind to an analyte in said sample to form an analyte/binding component complex, (iii) a third membrane pad that is a signal generation membrane pad with an immobilized binding component specific for and able to capture said analyte/binding component complex, (iv) a fourth membrane pad for absorption of flow medium; (b) a second group of membrane pads comprising: (vi) a fifth membrane pad for the supply of a substrate solution, (vii) a sixth membrane pad for absorption of excess substrate solution, and (c) substrate solution; wherein the system has a cross-arrangement of the two groups of membrane pads: said first group of membrane pads is arranged in a first flow direction wherein said first membrane pad is partially superimposed and fixed at an end of said second membrane pad, and said second membrane pad and said fourth membrane pad are partially superimposed and fixed one at each end of the third membrane pad, and wherein said sample is applied to said first membrane pad and flows laterally along said second and third membrane pads to said fourth membrane pad to comprise a first flow reaction; and said second group of membrane pads is arranged in a second flow direction perpendicular to the first flow direction, wherein said fifth and sixth membrane pads are partially superimposed and fixed at both lateral sides of the third membrane pad to form a pathway for a second flow reaction.