Patent ID: 7217544

Claim:
A method for manufacturing an L-amino acid from a D-amino acid comprising: a) reacting a recombinant microorganism with said D-amino acid, wherein: i) said recombinant microorganism has been made by transforming a starting microorganism with one or more vectors comprising polynucleotides encoding the enzymes: D-amino acid oxidase, wherein said D-amino acid oxidase is the D-amino acid oxidase of A. protophormiae; L-amino acid dehydrogenase, wherein said L-amino acid dehydrogenase is the L-amino acid dehydrogenase of Bacillus cereus ; and an enzyme that regenerates NADH, wherein said enzyme that regenerates NADH is the malate dehydrogenase from A. protophormiae; ii) relative to said starting microorganism, said recombinant microorganism overexpresses each of said enzymes; iii) the reaction is carried out under conditions in which each of said enzymes is active; and b) isolating said L-amino acid.