Patent ID: 6936442

Claim:
A method for detecting human parvovirus B19 in a biological sample, the method comprising: isolating nucleic acids from a biological sample suspected of containing human parvovirus wherein the nucleic acids are isolated from the biological sample by a method comprising (i) contacting a solid support comprising magnetic beads that comprise capture nucleic acids associated therewith with the biological sample under hybridizing conditions wherein target nucleic acid strands, if present in the biological sample, hybridize with the capture nucleic acids, and (ii) separating the solid support from the sample; amplifying the isolated nucleic acids using a sense and an antisense primer wherein (a) the sense primer consists of SEQ ID NO:88; (b) the antisense primer consists of SEQ ID NO:89; and detecting the presence of the amplified nucleic acids as an indication of the presence of human parvovirus B19 in the sample, wherein amplifying uses a fluorogenic 5′ nuclease assay using the sense primer and the antisense primer and detecting is done using at least one probe comprising a detectable label.