Patent ID: 7442524

Claim:
A method for producing a conjugate, said conjugate comprising (i) a polypeptide having clotting activity, said polypeptide comprising an amino acid sequence which (a) differs from the human Factor (hFVII) or human Factor VIIa (hFVIIa) sequence shown in SEQ ID NO:1 in no more than 15 amino acid residues and (b) comprises an introduced N-glycosylation site relative to SEQ ID NO:1, wherein the introduced N-glycosylation site comprises the substitution V253N, wherein amino acid residue positions in said polypeptide are numbered according to SEQ ID NO:1: and (ii) a sugar moiety covalently attached to the introduced N-glycosylation site, the method comprising: culturing a host cell comprising an expression vector comprising a nucleotide sequence which encodes said polypeptide under conditions conducive for the expression of the polypeptide, and recovering the polypeptide, wherein (a) the host cell is a eukaryotic host cell capable of in vivo glycosylation, and/or (b) the polypeptide is subjected to conjugation to a sugar moiety in vitro.