Patent ID: 7736644

Claim:
An isolated human monoclonal antibody that specifically binds to EGFRvIII, wherein EGFRvIII comprises a peptide that consists of the sequence L E E K K G N Y V V T D H C (SEQ ID NO: 56), and wherein the antibody comprises: a heavy chain variable region amino acid sequence selected from the group consisting of: SEQ ID NO: 142, and a variant of SEQ ID NO: 142 that has a point mutation selected from the group consisting of: His35Asn, Leu104Thr, Ala105Thr, Val50Gly, Val50Ala, Leu104Ile, Tyr52Glu, Tyr59Arg, Leu104Tyr, Val50His, His35Ser , His35Val, His35Ala, His35Gly, His35Thr, Tyr59Ser, Leu104Glu, Len104Gln , Tyr59His, Val50Asn, Tyr59Asn, Ala105Lys, Trp52Lys, Trp52Arg, Tyr59Thr, Leu104Ser, Leu104Asn, and Leu104Asp; and a light chain variable region amino acid sequence selected from the group consisting of: SEQ ID NO: 144; and a variant of SEQ ID NO: 144 that has a point mutation selected from the group consisting of; Leu99Glu, Leu99Asn, Leu99His, Leu99Thr, Gln98Glu, Asn35Gly, Asn35Arg, Leu99Tyr, Ala96Thr, Ala96Ser, Pro100Trp, Ser32Ile, His31Glu, Leu99Ser, Pro100Asp, Pro100Gln, Ser32Val, Ser32Pro, Pro100Gly, Pro100Ala, Ser32Ala, Ser32Thr, Pro100Ser, Gln98Thr, Pro100Met, Ser32Met, Ser32Gly, Pro100Asn, and Tyr37Phe, wherein if the heavy chain variable region is the variant of SEQ ID NO: 142 then the light chain variable region is SEQ ID NO: 144, and wherein if the light chain variable region is the variant of SEQ ID NO: 144, then the heavy chain variable region is SEQ ID NO: 142.