Patent ID: 8476072

Claim:
A set of genetic constructs, comprising: a) construct (i) comprising SEQ ID NO: 6, wherein construct (i) is configured to be stably integrated into the genome of at least one target cell; b) construct (ii) comprising SEQ ID NO: 22; and c) at least one construct selected from the group consisting of construct (iii), construct (iv), and construct (v), which respectively comprise components: C1-C2 (iii), C3 (iv), and C4 (v), wherein constructs (iii) and (iv) are nucleic acid molecules and construct (v) is an isolated or recombinant protein, wherein C1 is a promoter, C2 is an open reading frame (ORF) of a meganuclease, C3 is messenger RNA (mRNA) encoding of said meganuclease, and C4 is an isolated or recombinant protein of said meganuclease, wherein said meganuclease from constructs (iii), (iv), or (v) recognizes and cleaves a meganuclease cleavage site in construct (i), and wherein constructs (iii), (iv), or (v) are configured to be co-transfected with construct (ii) into said at least one target cell.