Patent ID: 7906301

Claim:
A method of generating a multiplexed biomolecule lysate, comprising the steps of: (a) contacting a first histopathologically processed biological sample with a first buffer solution and a second histopathologically processed biological sample with a second buffer solution; (b) heating said first histopathologically processed biological sample and second histopathologically processed biological sample at a temperature and for a time sufficient to substantially reduce the protein cross-links present in the biological samples to form heated first and second samples; (c) treating the heated first and second samples with an effective amount of one or more proteolytic enzymes for a time sufficient to substantially duce the proteins to individual peptides; and (d) separating the first sample contacted with said amount of one or more proteolytic enzymes using a first separation system and separating the second sample contacted with said amount of one or more proteolytic enzymes using a second separation system wherein the first separation system and the second separation system separately comprise a chromatography matrix; thereby generating said multiplexed biomolecule lysate.