Patent ID: 8420350

Claim:
A glycosylation-deficient hepatocyte growth factor (HGF) comprising a protein having at least a 90% sequence homology to SEQ ID NO: 2 and having the following amino acid substitutions: (a) substitution of amino acid 289 by Gln and/or amino acid 291 by Gly or Ala; (b) substitution of amino acid 397 by Gln and/or amino acid 399 by Gly or Ala; (c) substitution of amino acid 471 by Gly; (d) substitution of amino acid 561 by Gln and/or amino acid 563 by Gly or Ala; and (e) substitution of amino acid 648 by Gln and/or amino acid 650 by Gly or Ala; wherein the amino acid positions correspond to the sequence represented by SEQ ID NO: 2 and wherein there are no sugar chains at any of said amino acid positions corresponding to amino acids 289, 397, 471, 561, and 648.