Patent ID: 8648176

Claim:
A bispecific antibody comprising a (a) (i) a first polypeptide chain comprising the variable light chain region of an immunoglobulin that specifically binds a target molecule fused with or without a linker to a single chain variable fragment (scFv) that specifically binds a metal chelate comprising DOTA or an active variant thereof, wherein the scFv comprises the heavy and light chain CDR sequences of a mutant scFv comprising the amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, and 34; and (ii) a second polypeptide chain comprising a variable heavy chain region of the immunoglobulin of (a)(i) that specifically binds the target molecule; or (b) (i) a first polypeptide chain comprising the variable heavy chain region of an immunoglobulin that specifically binds a target molecule fused with or without a linker to a single chain variable fragment (scFv) that specifically binds a metal chelate comprising DOTA or an active variant thereof, wherein the scFv comprises the heavy and light chain CDR sequences of a mutant scFv comprising the amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, and 34; and (ii) a second polypeptide chain comprising the variable light chain region of the immunoglobulin of (b)(i) that specifically binds the target molecule.