Patent ID: 8502982

Claim:
A flow cell for use in an arrayed imaging reflectometry detection system, the flow cell comprising: first and second members that are secured together to define a substantially fluid-tight chamber having an inlet and an outlet, at least the second member being light transmissive; and a chip comprising a substrate, one or more coating layers on the substrate, and one or more probe molecules tethered to the outermost coating layer, the one or more coating layers are suitably dimensioned to create a condition of near perfect destructive interference upon exposure to s-polarized light of a suitable wavelength at a specific incidence angle, and in the absence of the one or more target molecules binding to the one or more probe molecules, the chip being positioned with the outermost coating layer and the one or more probe molecules thereon exposed to aqueous fluid in the chamber and facing the second member, whereby a substantial change in s-polarized light reflectivity from the chip occurs in the presence of the one or more target molecules.