Patent ID: 7303900

Claim:
A method for the production of tyrosine comprising: a) providing a recombinant microorganism comprising at least one nucleic acid fragment encoding a phenylalanine hydroxylase polypeptide, wherein said at least one nucleic acid fragment encoding a phenylalanine hydroxylase polypeptide is selected from the group consisting of: 1) an isolated nucleic acid fragment isolated from Pseudomonas genomic DNA by a polymerase chain reaction protocol using any two primers selected from the group consisting of SEQ ID NOS:7-16 and the following amplification conditions: i) 1.0 mg of the genomic DNA template, ii) 2.5 units of Taq DNA polymerase in 10 mM Tris-HCl, iii) 0.2 mM each of the four dNTPs, 50 mM KCl, 1.5 mM MgCl2, and 0.01% bovine serum albumin, and iv) thermalcycling reaction of 94° C., 0.5 min; 55° C., 0.5 min and 72° C., 2.0 mm, and 2) an isolated nucleic acid fragment encoding the polypeptide as set forth in SEQ ID NO:2; and b) growing said recombinant microorganism in the presence of a fermentable carbon substrate whereby tyrosine is produced.