Patent ID: 8034608

Claim:
A method of promoting endothelialization of a hybrid vascular graft consisting of a hemodialysis access graft or femoral artery graft, said method comprising: (a) immobilizing a plurality of multipotent stem cells on at least one surface of a specimen comprising a hybrid hemodialysis access vascular graft or a hybrid femoral artery vascular graft; and (b) placing said specimen in a system under conditions effective to promote said stem cells to form a confluent monolayer on said surface of said specimen, wherein said system comprises: a controller having at least one input for receiving information and feedback information and an output for outputting control signals; and a pressure/flow loop subsystem coupled to said controller, comprising: a specimen holder, an external flow loop system coupled to said specimen holder, and an output for outputting said feedback information; wherein said pressure/flow loop subsystem receives said control signals and is capable of independently controlling pressure and flow rate at said specimen in accordance with said control signals, when said specimen holder contains said specimen.