Patent ID: 6936706

Claim:
A polynucleotide, comprising a nucleic acid encoding a polypeptide that selectively binds the human milk fat globule (HMFG) antigen(s), and competes as well or better with the unmodified antibody for binding the HFMG antigen(s), wherein the polypeptide comprises a non-HMFG antigen(s)-binding peptide from a first species, and an HMFG antigen(s)-binding peptide comprising light and heavy chains of the variable region of an antibody of a second species, wherein at least one chain of the non-HMFG antigen(s)-binding peptide is linked to the HMFG antigen(s)-binding peptide, the chains may be linked to one another at a site other than the antigenic binding site, and at least one chain of the HMFG antigen(s)-binding peptide has 1 to 46 amino acids substituted with at least one amino acid selected from the following amino acids at the specific sites and chains indicated: Light Chain Heavy Chain Position Amino Acid Substitution Amino Acid Substitution 1 D PVLKARGHDE or Q 2 WKCRHV or I M or V 3 V or L LQR or K 4 MPTQLV or I M or L 5 T VD or E 6 Q QD or E 7 TIAD or S T or S 8 PA or E G or E 9 LF or P G 10 T or S DA or G 11 NL or V VF or L 12 S or P I or V 13 V KE or Q 14 S or T P 15 PFI or L G 16 G RSE or G 17 TEQ or D PA or S 18 PS or Q M 19 V or A R or K 20 S V or L 21 I S 22 S C 23 C TSE or A 24 V or A 25 S 26 G 27 F 28 AINS or T 29 F 30 S 35 W 36 IVHNYF or L W 37 WLVKRTHDE or Q WIFLVMATG or Q 38 Q R 39 R or K Q 40 FLKARTGQP or S VA or S 41 G PLVARTSHNE or Q 42 Q G or E 43 P or S K 44 P SR or G 45 EQR or K L 46 RV or L Q or E 47 WILMTSN or V W 48 FPLVMTS or I STG or V 49 Y or S A 57 WVTSGDNE or Q 58 IYFLVMAT or Q 59 S or P 60 N or D 61 T or R 62 F 63 IYPLKARSG or T 64 D or G 65 S 66 G R 67 A or S F 68 VMCARSGQD or E IS or T 69 T I 70 D L or S 71 F R 72 T VKRTSGHDE or N 73 L N or D 74 NLRE or K FPLVTGDNA or S 75 L or I EN or K 76 IT or S NTRK or S 77 S or R TNIVSM or R 78 ALI or V LA or V 79 KGQ or E FH or Y 80 P or A L 81 ILVKMAGDN or E E or Q 82 D M 82a SDN or I 82b IR or S 82c PLVMAG or E 83 MV or L EKT or R 84 LVARTS or G SPVTI or A 85 IM or V WIYFPLMCRGD or E 86 Y D 87 IMSHEFY or L M or T 88 C A or G 89 ITVM or L 90 HP or Y 91 Y 92 C 93 ASHAV or T 94 TSDQLAWR or G 98 F 99 G 100 IPVKRTGAS or Q 101 G 102 T 103 IYMATGHDEQNR or K A or W 104 LG or V YH or G 105 ILVTSGHNE or Q THR or Q 106 YLVKMRTD or I G 106a PLVTI 107 ILVMATSGNER or K AQ or T 108 STGM or L 109 IKLT or V 110 SL or T 111 V 112 T or S and . . . , or single chain thereof.