Patent ID: 7341845

Claim:
A method for identifying a compound as a kinesin inhibitor, comprising the steps of: (a) contacting said compound with a first kinesin or fragment thereof, wherein said first kinesin or fragment thereof comprises a motor domain and a L5 loop; (b) determining an inhibition of activity of said first kinesin or fragment thereof; (c) contacting said compound with a hybrid kinesin or fragment thereof, wherein said hybrid kinesin or fragment thereof comprises a motor domain from said first kinesin and a L5 loop from a second kinesin, and wherein said second kinesin is different from said first kinesin; and (d) determining an inhibition of activity of said hybrid kinesin or fragment thereof, wherein a difference in the inhibition of activity measured in step (b) and step (d) indicates binding of said compound to said L5 loop from said first kinesin or fragment thereof, and wherein a similarity in the inhibition of activity measured in step (b) and step (d) indicates binding of said compound outside said L5 loop from said first kinesin or fragment thereof.