Patent ID: 8143347

Claim:
A method of conjugating a water soluble polymer (WSP) to Factor VIII (FVIII) comprising: (a) incubating FVIII with FVIII-specific antibody under conditions that allow binding of said antibody to said FVIII to form an antibody: FVIII complex; (b) incubating the antibody: FVIII complex with said WSP under conditions that allow conjugation of the WSP to the antibody: FVIII complex; and (c) releasing the WSP-conjugated FVIII from the antibody, and wherein the WSP is selected from the group consisting of polyethylene glycol (PEG), branched PEG, polysialic acid (PSA), carbohydrate, polysaccharides, pullulane, chitosan, hyaluronic acid, chondroitin sulfate, dermatan sulfate, starch, dextran, carboxymethyl-dextran, polyalkylene oxide (PAO), polyalkylene glycol (PAG), polypropylene glycol (PPG) polyoxazoline, poly acryloylmorpholine, polyvinyl alcohol (PVA), polycarboxylate, polyvinylpyrrolidone, polyphosphazene, polyoxazoline, polyethylene-co-maleic acid anhydride, polystyrene-co-maleic acid anhydride, poly(1-hydroxymethylethylene hydroxymethylformal) (PHF), and/or 2-methacryloyloxy-2′-ethyltrimethylammoniumphosphate (MPC).