Patent ID: 7955819

Claim:
A process for preparing asparagine-linked oligosaccharide derivatives, comprising the steps of: (a) treating a delipidated egg yolk with orientase in a solution to obtain a mixture containing peptide-linked oligosaccharides from said egg yolk; (b) subjecting the mixture obtained in step (a) to gel filtration column chromatography to isolate the peptide-linked oligosaccharides from the mixture; (c) treating the peptide-linked oligosaccharides isolated in step (b) with actinase in a solution to obtain a mixture containing asparagine-linked oligosaccharides; and (d) subjecting the mixture obtained in step (c) to gel filtration column chromatography to isolate the asparagine-linked oligosaccharides from the mixture, and introducing a lipophilic protective group to the isolated asparagine-linked oligosaccharides by adding a solution containing the lipophilic protective group to said oligosaccharides to prepare asparagine-linked oligosaccharide derivatives.