Patent ID: 8586312

Claim:
An array, comprising: a support comprising a first discrete region and a second discrete region, wherein the first discrete region and the second discrete region are configured to be separately addressable; a pool of probes bound to the first discrete region comprising a first component probe and a second component probe respectively complementary to non-overlapping segments of a mRNA molecule, wherein the pool of probes is configured such that hybridization of the pool of probes with the mRNA molecule or a nucleic acid copied therefrom does not allow individual probes of the pool of probes to be separately addressable; and a mismatch probe, or pool of mismatch probes, bound to the second discrete region, wherein the mismatch probe has the same sequence as one of the first component probes and second component probes except for a single base mismatch, wherein the pool of mismatch probes comprises a first mismatch probe and a second mismatch probe that respectively have the same sequence as the first component probe and second component probe except for a single base mismatch, and wherein the pool of mismatch probes is configured such that hybridization of the pool of mismatch probes with the mRNA molecule or the nucleic acid copied therefrom does not allow individual mismatch probes to be separately addressable.