Patent ID: 6864071

Claim:
A method for the amplification of at least one target nucleic acid sequence of interest, comprising, for each target nucleic acid sequence of interest: (a) contacting upstream and downstream oligonucleotide ligation probes with the target nucleic acid sequence such that the 3′ terminus of the upstream probe is juxtaposed to the 5′ terminus of the downstream probe while both the upstream and downstream probes are in contact with the target nucleic acid sequence; (b) ligating together the upstream and downstream probes at their respective juxtaposed termini to form a ligated target probe template; and (c) using the ligated target probe template in a strand displacement amplification (“SDA”) reaction to form amplicons of the ligated target probe template, wherein the strand displacement reaction utilizes at least a first SDA primer, each SDA primer comprising a restriction endonuclease sequence on the 5′ region of the primer, and no bumper primers are used in the strand displacement amplification, wherein steps (a), (b), and (c) exit in solution at the same time.