Patent ID: 7300752

Claim:
A method for determining the gender of a subject from the canis familiaris species, comprising: a) contacting a nucleic acid sample from the subject with a first and a second oligonucleotide primer, wherein the first and/or second oligonucleotide primer is complementary to consensus regions between SEQ ID NO:22 and SEQ ID NO:23, and wherein such first and second primers flank non-consensus regions between SEQ ID NO:22 and SEQ ID NO:23; b) amplifying the flanked non-consensus regions, wherein the flanked non-consensus regions of SEQ ID NO:22 contain at least one gap in sequence alignment compared to the flanked non-consensus regions of SEQ ID NO:23, such that different length amplification products result if sequences comprising both SEQ ID NO:22 and SEQ ID NO:23 are present in the sample; and c) detecting amplification products resulting from step (b), wherein detection of amplification products of different lengths correlates with male gender.