Patent ID: 8530630

Claim:
A method for purification of a gamma-carboxy-glutamic (GLA)-residue containing serine protease in solution, said method comprising: subjecting the solution to one or more purification steps selected from the group consisting of anion exchange chromatography, hydrophobic interaction chromatography, immunoaffinity purification, size exclusion chromatography, diafiltration, and ultrafiltration, adjusting calcium in the solution to obtain a molar ratio of free calcium ions and Factor VII polypeptide (Ca2+:FVII polypeptide) lower than 0.5; and adjusting the pH of the solution to a value between 4.5 and 6.9 or to a value between 8.6 and 10, wherein said GLA-residue containing serine proteases comprises a Factor VII polypeptide.