Patent ID: 8053179

Claim:
A method for manufacturing a substrate for making a microarray, comprising at least a step of forming a resist film on the substrate using a chemically amplified positive resist composition using a copolymer where a content of a monomer unit having a hydroxyl group is 5 mole % or less relative to total monomer units as a binder; a step of patterning the resist film; a step of forming a monomolecular film having a silicon oxide chain on the substrate having the patterned resist film at positions other than on the patterned resist film, the monomolecular film for immobilizing a target molecule; and subsequently a step of removing the patterned resist film to obtain a substrate for making a microarray, wherein the monomolecular film is formed by treating the substrate having the patterned resist film with a treating solution containing a silicon compound represented by the following general formula (5): Y 3 Si—(CH 2 ) m —X (5) wherein m represents an integer of 3 or more, X denotes any one of an amino group, a hydroxyl group protected with a protecting group, and a formyl group protected with a protecting group, and Y independently denotes a halogen atom or an alkoxy group having 1-4 carbon atoms.