Patent ID: 7268274

Claim:
A method of improving glyphosate tolerance in a wheat plant comprising: (1) constructing a DNA construct comprising a first and a second expression cassette, wherein said first expression cassette in operable linkage comprises (i) a rice actin 1 promoter; (ii) a rice actin 1 intron; (iii) a chioroplast transit peptide encoding DNA molecule; (iv) a glyphosate tolerant EPSPS encoding DNA molecule; and (v) a transcriptional terminator DNA molecule; and said second expression cassette comprising in operable linkage (a) a CaMV 35S promoter; (b) a Hsp70 intron; (c) a chloroplast transit peptide encoding DNA molecule; (d) a glyphosate tolerant EPSPS encoding DNA molecule; and (e) a transcriptional terminator DNA molecule; and (2) transforming a wheat cell with said DNA construct; and (3) regenerating said wheat cell into a wheat plant or wheat plants; and (4) treating said wheat plants with an effective dose of glyphosate; and (5) selecting fertile wheat plants that are vegetative and reproductive tolerant to glyphosate.