Patent ID: 7943323

Claim:
A method for producing an antibody for specifically detecting whole PTH 1-84 while avoiding detecting an interfering non-(1-84) parathyroid hormone fragment in a biological sample, which method comprises: a) administering a first peptide or protein immunogen to a host animal to induce antibody production against said first peptide or protein immunogen in said host animal, said first peptide or protein immunogen being human, rat, bovine, or porcine PTH 1-84 ; b) monitoring antibody titer produced by said administration of said first peptide or protein immunogen to said host animal; c) extracting antiserum produced in said host animal by said administration of said first peptide or protein immunogen; and d) purifying said antiserum and selecting an antibody from said antiserum extracted in step c) by affinity chromatography utilizing a second peptide or protein immunogen, which is human PTH 1-8 , rat PTH 1-8 , or a peptide having at least four amino acids in the common sequence of human and rat PTH 1-8 .