Patent ID: 7985542

Claim:
A method of detection of the presence of at least one domain of interest on a macromolecule to test, wherein said method comprises the following steps: a) determining at least three target regions on the domain of interest, b) obtaining a corresponding labeled set of at least three probes each probe targeting one of said target region, the position of the probes one compared to the others being chosen and forming a sequence of at least two codes chosen between a group of at least two different codes, said sequence of codes being specific of the domain and being a specific signature of said domain of interest on the macromolecule to test; c) spreading the macromolecule and binding the probes to the macromolecule, wherein the spreading step occurs before or after the binding step, d) reading signals given by each of the labeled probes, each signal being associated with the label of said one probe, e) transcribing said signals in a sequence of codes established from the gap size between consecutive probes, f) detecting the sequence of codes of a domain of interest said sequence indicating the presence of said domain of interest on the macromolecule to test, and conversely the absence of detection of sequence of codes or part of sequence of codes of a domain of interest indicating the absence of said domain or part of said domain of interest on the macromolecule to test.