Patent ID: 8206989

Claim:
A method of generating a dosing schedule for a test compound known or suspected to modulate Notch processing in vivo mediated by γ-secretase comprising: (a) determining a baseline amount of Trefoil factor-3 (TFF-3) in a sample acquired from a query subject in the absence of a test compound; (b) administering a first dose of the test compound to the query subject, wherein said test compound is a known γ-secretase modulator or suspected γ-secretase modulator; (c) determining an amount of TFF-3 and a Notch-related toxicity level in samples acquired from the query subject of step (b) at multiple time points after administration of the test compound; (d) repeating steps (b) and (c) for one or more additional doses of the test compound; and (e) generating a dosing schedule by comparing TFF-3 amounts and associated Notch-related toxicity levels at the multiple doses and time points.