Patent ID: 7951890

Claim:
A method of extracting steroid compounds using entrapped β-cyclodextrin polymers, the method comprising: 1) adding a buffer solution and a hydrolase to a sample containing steroid compounds and reacting the mixture; 2) adding entrapped β-cyclodextrin polymers to the mixture, stirring the mixture, and centrifuging the mixture to isolate entrapped β-cyclodextrin polymers and form a water layer, wherein the entrapped β-cyclodextrin polymer is prepared by immersing the β-cyclodextrin polymers in a gel state in a metal salt solution followed by entrapping on the surface of the β-cyclodextrin polymers, wherein the metal salt is selected from the group consisting of CaCl 2 , NaCl, Ca(NO 3 ) 2 and CaSO 4 3) removing water layer formed in step 2); and 4) extracting the steroid compounds by adding a phosphate buffer solution and a polar organic solvent to the mixture after removing the water layer, adjusting the pH of the mixture in the range of 9.0 to 10.0, and adding an organic solvent to the mixture.