Patent ID: 8137935

Claim:
A method for isolating one or more endotoxins, comprising: suspending a group of bacterial cells or a culture supernatant, wherein the bacterial cells or the culture supernantant contain the endotoxins, in a mixture of solvents containing: 10 to 90% of a solvent chosen from: linear or branched aliphatic acids containing from 3 to 6 carbon atoms, and 90 to 10% of a basic aqueous solution of an aliphatic amine containing from 0 to 12 carbon atoms; stirring the suspended bacterial cells or a culture supernatant to produce a solution of bacterial endotoxins in the mixture of solvents containing suspended particles of cell debris; and separating the particles from the solution by (1) filtration, (2) centrifugation and recover of the supernatant or (3) centrifugation followed by filtration of the supernatant, to produce the isolated endotoxins, wherein the endotoxins are not obtained in combination with silica.