Patent ID: 7214536

Claim:
A method for introducing a double-stranded break into a target nucleic acid sequence in a plant or animal cell comprising: providing an isolated plant or animal cell comprising a Group I intron encoded endonuclease recognition site in a target nucleic acid and providing the Group I intron encoded endonuclease to the cell by genetically modifying the cell with a nucleic acid comprising said Group I intron encoded endonuclease or introducing said Group I intron encoded endonuclease protein into the cell such that the endonuclease cleaves the endonuclease site in the target nucleic acid sequence, wherein the site is selected from the group consisting of an I-SceIV site, an I-CsmI site, an I-PanI site, an I-SceII site, an I-CeuI site, an I-PpoI site, an I-SceIII site, an I-CreI site, an I-TevI site, an I-TevII site, an I-TevIII site, and an I-SceI site.