Patent ID: 8268596

Claim:
A process of making an altered Neisserial oligosaccharide structure comprising the steps of deleting an open reading frame encoding a glycosyltransferase from a Neisserial strain selected from the list consisting of: LgtA (Lipooligosaccharide glycosyl transferase A), the Neisseria gonorrhoeae strain F62 amino acid sequence of which being SEQ ID NO:2, LgtB, the Neisseria gonorrhoeae strain F62 amino acid sequence of which being SEQ ID NO: 3, LgtC, the Neisseria gonorrhoeae strain F62 amino acid sequence of which being SEQ ID NO:4, LgtD, the Neisseria gonorrhoeae strain F62 amino acid sequence of which being SEQ ID NO:5, and LgtE, the Neisseria gonorrhoeae strain F62 amino acid sequence of which being SEQ ID NO:6; and preparing the altered Neisserial oligosaccharide structure from said strain.