Patent ID: 7192784

Claim:
A chromatography measuring method comprising: providing a biosensor having (i) a development portion, (ii) a label reagent held in a first part of said development portion in a dry state, said label reagent being elutable by a test sample comprising an analyte, and (iii) an immobilized reagent in a second part of said development portion, wherein said label reagent in said first part binds to said analyte in said test sample, and said immobilized reagent in said second part is a reagent that specifically binds to a complex comprising said analyte and the labeled reagent; developing said test sample on said development portion; using an optical detector to measure in an area between said first and second parts of said development portion that does not include said second part of said development portion, an amount of said label reagent eluted from said first part of said development portion; using an optical detector to measure, in said second part of said development portion, an amount of any label reagent bound to said immobilized reagent in said second part of said development portion; correcting the measured amount of said label reagent bound to said immobilized reagent in said second part of said development portion, on the basis of a relationship between the measured amount of said label reagent eluted from the first part of said development portion and a total amount of said label reagent present in said first part of said development portion before application of said test sample; and calculating a concentration of said analyte in said test sample on the basis of the corrected amount of said label reagent bound to said immobilized reagent in said second part of said development portion.