Patent ID: 7037721

Claim:
A protein-free defined medium for culturing epidermal keratinocytes comprising: a) N-(2-OH-ethyl-)piperazine-N-(2-ethane-sulfonic acid) at a concentration of 14-22 mM; b) sodium chloride at a concentration of 100-120 mM; c) histidine at a concentration of 0.1-0.25 mM; d) isoleucine at a concentration of 0.05-0.5 mM; e) methionine at a concentration of 0.1-0.5 mM; f) phenylalanine at a concentration of 0.1-0.5 mM; g) tryptophan at a concentration of 0.05-0.5 mM; h) tyrosine at a concentration of 0.1-0.5 mM; and i) retinyl acetate at a concentration of 0.3-33 ng/ml.