Patent ID: 8772011

Claim:
A variant of a parent protease, comprising a substitution selected from the group consisting of: 6C; 7P; 8C; 9C; 10E,D; 12E,D; 13E,D,P; 16C; 17C; 18C; 23P; 24K,P,R; 25K,P,R; 27P; 32C; 33C; 37C; 39R,K; 42E,D; 43W; 46D,E; 47D,E; 52C; 55C; 56R,K; 58E,D; 62C; 63C; 67C; 69D,E; 70D,E; 71C; 72K,R; 76C; 81D,E,P; 82D,E,P; 84D,E; 85C; 89C,D,E; 92P,R; 93P; 94C,P; 95E,D; 96E,D,P; 97R,K; 98P; 99R,K; 103C; 105C,P; 106C; 111R,K; 113E,D; 118R,K; 120E,D; 122K; 124R,K; 125P; 127K; 129E,D; 130E,D; 134C; 135P; 136P; 139C; 140E,D; 141C; 144C; 145C; 146C; 147W; 148C; 149C; 150E,D; 151P,E,D; 155C; 156C; 160D,E; 161D,E; 162K,R; 163C; 166D,E; 170C; 172C; 173C; 174P; 175P; 176P; 180R,K; 181R,K; 184P; 187P; and 188R,K wherein (a) the variant has protease activity; (b) each position corresponds to a position of amino acids 1 to 188 of SEQ ID NO: 2; and (c) the variant has a sequence identity to amino acids 1 to 188 of SEQ ID NO: 2 of at least 90%.