Patent ID: 7176008

Claim:
A process for purifying TAFIa from a serum sample comprising the sequential steps of a) adding a reversible inhibitor of TAFIa to the serum sample to form a first mixture; b) adding a coagulation initiator to the first mixture to convert TAFI to TAFIa and form a second mixture; c) applying the second mixture to a heparin-sepharose column to bind TAFI and TAFIa to the column; d) washing the heparin-sepharose column to remove proteins not bound to the heparin-sepharose column; e) eluting the bound TAFI and bound TAFIa from the heparin-sepharose column to form a third mixture; f) applying the third mixture to a TAFIa isolation column to bind TAFIa to the isolation column; g) washing the TAFIa isolation column to remove proteins not bound to the TAFIa isolation column; and h) eluting the bound TAFIa from the TAFIa isolation column.