Patent ID: 7943334

Claim:
A method for detecting albumen allergens in a sample by sandwich ELISA, which method comprises the following steps (a) to (c): (a) preparing an immune complex by allowing an albumen allergen in the sample to contact a first anti-native ovalbumin monoclonal antibody recognizing a native ovalbumin, bound to an insolubilized carrier, and a first anti-reduced carboxymethylated ovalbumin monoclonal antibody recognizing a denatured ovalbumin, bound to the same insolubilized carrier; (b) preparing a labeled immune complex by allowing the immune complex prepared in step (a) to contact a labeled second anti-native ovalbumin monoclonal antibody recognizing a native ovalbumin and a labeled second anti-reduced carboxymethylated ovalbumin monoclonal antibody recognizing a denatured ovalbumin, wherein the second monoclonal antibody recognizing a native ovalbumin recognizes a different epitope of native ovalbumin than the epitope recognized by the first monoclonal antibody recognizing a native ovalbumin, and the second monoclonal antibody recognizing a denatured ovalbumin recognizes a different epitope of denatured ovalbumin than the epitope recognized by the first monoclonal antibody recognizing denatured ovalbumin; and (c) detecting native and denatured albumen allergen in the sample by detecting the labeled immune complex prepared in step (b).