Patent ID: 7531354

Claim:
A method for producing adherent feeder cell free neuroprogenitor cells, said method comprising the steps of: a. contacting adherent primate embryonic stem cells in a serum free differentiation medium consisting essentially of at least the components of a minimum essential medium and basic fibroblast growth factor (bFGF) and optionally non-essential amino acids, leukaemia inhibitory factor (LIF), an antibiotic, mercaptoethanol or another cell medium selected from B27, N2 or mixtures thereof, with an effective amount of at least one differentiation protein to differentiate said adherent primate embryonic stem cells without embryoid cell formation into adherent neuroprogenitor cells which are greater than 90% nestin positive, and which are PSNCAM and A2B5 negative; b. passaging said neuroprogenitor cells; and c. isolating or collecting said neuroprogenitor cells.