Patent ID: 8895709

Claim:
A method for producing a host cell-protein (HCP) reduced anti-IL-12 antibody, or antigen binding portion thereof, preparation from a sample mixture comprising an anti-IL-12 antibody, or antigen binding portion thereof, and at least one HCP, said method comprising: (a) subjecting said sample mixture to a reduction in pH thus forming a reduced pH primary recovery sample, wherein said reduction in pH is from about 3 to about 4; (b) adjusting said reduced pH primary recovery sample to a pH of about 6.0 to about 8 thus forming an adjusted pH primary recovery sample followed by; (c) contacting said adjusted pH primary recovery sample to a Protein A affinity chromatography resin, washing said affinity chromatography resin with a buffer comprising 0.5 M NaCl, 20 mM citric acid/Na citrate, at pH 6, and collecting an affinity chromatography sample; (d) contacting said affinity chromatography sample to an ion exchange resin and collecting an ion exchange sample; (e) contacting said ion exchange sample to a hydrophobic interactive chromatography (HIC) resin and collecting an HIC sample, wherein said HIC sample comprises said HCP-reduced anti-IL-12 antibody, or antigen binding portion thereof, preparation.