Patent ID: 8323467

Claim:
A dual chamber, multi-analyte test strip comprising: a first insulating layer; a first electrically conductive layer disposed on the first insulating layer, the electrically conductive layer including a first working electrode with a first analyte contact pad; a first patterned spacer layer positioned above the first electrically conductive layer, the patterned spacer layer defining a first sample-receiving chamber therein that overlies the first working electrode, the first sample-receiving chamber having a first end opening and a second end opening; a first counter/reference electrode layer overlying and exposed to the first sample receiving chamber, the first counter/reference electrode layer configured in an opposing relationship to the first working electrode and having a first counter/reference contact pad; a counter/reference insulating layer disposed over the first counter/reference electrode layer, a second counter/reference electrode layer disposed over the counter/reference insulating layer the second counter/reference electrode layer having a second counter/reference contact pad, a second patterned spacer layer positioned above the second counter/reference electrode layer, the second patterned spacer layer defining a second sample-receiving chamber therein with a first end opening and a second end opening; a second electrically conductive layer disposed above the second patterned spacer layer, the second electrically conductive layer including a second working electrode with a second analyte contact pad; a second insulating layer disposed above the second electrically conductive layer; a first analyte reagent layer disposed on at least a portion of the first working electrode within the first sample-receiving chamber; and a second analyte reagent layer disposed on at least a portion of the second working electrode within the second sample-receiving chamber; wherein the second sample-receiving chamber overlies the second working electrode, and wherein the second counter/reference electrode layer is exposed to the second sample receiving chamber and configured in an opposing relationship to the second working electrode.