Patent ID: 8647823

Claim:
A method of producing a polynucleotide using a solid phase polymerase chain reaction (PCR), said method comprising: (i) contacting a solid substrate comprising a primer that is attached to the solid substrate surface through a linker that is covalently attached to the solid substrate with a solution comprising a polymerase, dNTP or NTP, and a template DNA or RNA under polymerase chain reaction conditions sufficient to produce a polynucleotide that is complementary to the template DNA or RNA; (ii) contacting a solid substrate comprising a template DNA or RNA that is attached to the solid substrate surface through a linker with a solution comprising a polymerase, dNTP or NTP, and a primer under polymerase chain reaction conditions sufficient to produce a polynucleotide that is complementary to the template DNA or RNA; or (iii) contacting a solid substrate comprising a polymerase that is attached to the solid substrate surface through a linker with a solution comprising a primer, dNTP or NTP, and a template DNA or RNA under polymerase chain reaction conditions sufficient to produce a polynucleotide that is complementary to the template DNA or RNA; wherein the linker comprises a dendron that comprises a plurality of termini covalently attached to the solid substrate surface and a single linear region that is attached to the primer, the template DNA or RNA, or the polymerase of (i), (ii), or (iii), respectively, and wherein attachment of the linker to the solid substrate provides a significantly higher thermal stability to the PCR conditions such that when the solid substrate is a glass slide, the linker provides more than twice the thermal stability compared to aminosilane treated glass slide.