Patent ID: 8853379

Claim:
An isolated polynucleotide comprising a coding sequence that encodes a chimeric polypeptide, the chimeric polypeptide comprising at least one subdomain of an E protein of a dengue virus bound to at least one subdomain of a membrane M protein of a dengue virus, wherein the subdomain of the E protein comprises at least one ectodomain III peptide selected from amino acids 18 to 120 of SEQ ID NO: 20, amino acids 171 to 273 of SEQ ID NO: 20, amino acids 324 to 426 of SEQ ID NO: 20, and amino acids 477 to 579 of SEQ ID NO: 20, and wherein the subdomain of the membrane M protein comprises at least one peptide selected from amino acids 123 to 162 of SEQ ID NO: 3, amino acids 154 to 162 of SEQ ID NO: 3, and amino acids 122 to 132 of SEQ ID NO: 12.