Patent ID: 7855281

Claim:
A method comprising: contacting a synthetic polynucleotide that is covalently bound at a terminal nucleotide of the synthetic polynucleotide to a substrate via a cleavable linker with a solution comprising an α-effect nucleophile to result in cleavage of the synthetic polynucleotide from the substrate; wherein the cleavable linker has the structure (I): wherein: Lnk is a linking group; Sub denotes the site at which the substrate is attached to the cleavable linker; and RPN is a 2′-, 3′- or 5′-position of the terminal nucleotide of the synthetic polynucleotide; and wherein the cleaved synthetic polynucleotide comprises a hydroxyl group at the 2′-, 3′- or 5′-position of the terminal nucleotide.