Patent ID: 8607612

Claim:
A method of calibrating a reversible-binding sensor for detecting an analyte, which method comprises: varying the temperature of a first calibration solution from a first temperature (T 1 ) to a second temperature (T 2 ) while the first calibration solution is in contact with a sensing region of the sensor, wherein the first temperature (T 1 ) is different than the second temperature (T 2 ); detecting a sensor output for each of a plurality of temperatures between the first temperature (T 1 ) and the second temperature (T 2 ) during the varying of the temperature; determining the sensor output for the first calibration solution as a function of temperature; varying the temperature of a second calibration solution from a third temperature (T 3 ) to a fourth temperature (T 4 ) while the second calibration solution is in contact with the sensing region, the second calibration solution having a concentration of analyte which is different from that of the first calibration solution, wherein the third temperature (T 3 ) is different than the fourth temperature (T 4 ); detecting a sensor output for each of a plurality of temperatures between the first temperature (T 3 ) and the second temperature (T 4 ) during the varying of the temperature; determining the sensor output for the second calibration solution as a function of temperature; and using the determined sensor output for the first calibration solution and the second calibration solution, each as a function of temperature, to calibrate the sensor.