Patent ID: 7704721

Claim:
A method of preventing aggregation of recombinant adeno-associated virus (rAAV) virions in a purified preparation of rAAV virions, comprising: 1) providing a lysate comprising rAAV virions; 2) purifying rAAV virions from the lysate using ultracentrifugation and/or chromatography, wherein said virions are purified; and 3) adding one or more salts of multivalent ions selected from the group consisting of citrate, phosphate, sulfate and magnesium to said purified virions to produce a preparation of virions with an ionic strength of at least 200 mM, wherein the concentration of purified rAAV virions in said preparation exceeds 1×10 13 vg/ml up to 6.4×10 13 vg/ml; and wherein the pH of the purified preparation of rAAV virions is between 7.5 and 8.0.