Patent ID: 7262021

Claim:
A method of antimicrobial-susceptibility testing microbes present in a sample, the method comprising the steps of: applying a portion of the sample containing microbes to a culture plate containing agar-based media; applying a fraction of an antimicrobial susceptibility test disk to the culture plate such that the fraction abuts at least one wall of the plate, wherein the fraction is approximately one quarter or one half of the test disk and the flat edge of the quarter disk or the half disk abuts a wall of the plate or the flat edges of the quarter disk abut adjacent corner walls of the plate; incubating the culture plate; determining the presence of a zone of inhibition about the fraction of the antimicrobial test disk; measuring a radius of the zone of inhibition and multiplying the radius by a factor of two to obtain an inhibition diameter value; and comparing the inhibition diameter value obtained with standardized disk diffusion antimicrobial-susceptibility interpretation charts to provide antimicrobial-susceptibility information about the sample.