Patent ID: 8679829

Claim:
An isolated or a purified sulfolobus expression vector comprising: (a) a sulfolobus origin of replication; (b) coding sequences for structural proteins, a coding sequence for a site-specific integrase and a packaging signal wherein each of the structural protein coding sequences, the site-specific integrase coding sequence and the packaging signal are from one of SSV1, SSV2 or pSSVx and are operably linked to expression control sequences; (c) one or more selectable marker gene(s) encoding one or more essential proteins of sulfolobus, operatively linked to sulfolobus expression control sequences, wherein the essential proteins are orotidine-5′-monophosphates pyrophosphorlyase and orotidine-5′-monophosphatase decarboxylase; and (d) a sulfolobus promoter followed 3′ by a restriction enzyme recognition site or a multiple cloning site within which, or adjacent to, is an inserted gene of interest, and the vector further comprises an optional 3′ regulatory element.