Patent ID: 8338571

Claim:
A recombinant factor VIII comprising one or more mutations that result in enhanced stability of both factor VIII and factor VIIIa, wherein the one or more mutations are selected from the group consisting of substitution of a Glu residue with a hydrophobic amino acid residue at a position corresponding to amino acid 287, 665, and/or 1984 of SEQ ID NO: 2, substitution of an Asp residue with a hydrophobic amino acid residue at a position corresponding to amino acid 302 and/or 519 of SEQ ID NO: 2, and combinations of two or more of said substitutions, and wherein the position of the substitution or substitutions of said recombinant factor VIII aligns with amino acids 287, 302, 519, 665, and/or 1984 of SEQ ID NO: 2 upon alignment of the amino acid sequence of the recombinant factor VIII with the amino acid sequence of SEQ ID NO: 2.