Patent ID: 8557147

Claim:
A chemiluminescent composition, comprising a 1,2-dioxetane compound having the structure: wherein R 1 is selected from alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, heterocycloalkyl, alkyloxyalkyl, alkyloxyaryl, alkyloxyhaloalkyl, alkylalkenyl, alkylalkynyl, halogenated alkyl, alkyl alcohol, alkyl carbonitrile, alkyl amine, alkyl acid, halogenated alkyl alcohol, halogenated alkyl carbonitrile, halogenated alkyl amine, or halogenated alkyl acid; X is hydrogen or a substituent on phenyl ring; Y is hydrogen, alkyl, acetoxyl, t-butyldimethylsilyl, a group capable of being cleaved by an enzyme, or a group capable of being cleaved by an antibody; and Z is hydrogen or a substituent on adamantane ring; at least one alkyl dimethyl benzyl quaternary ammonium salt having the structure of General Formula I: wherein: R is selected from C 8-20 alkyl or C 8-20 alkenyl; X − is a negative ion; Y is a benzene ring substituent, each of which is independently selected from: hydrogen, halogen, hydroxyl, sulfhydryl, cyano, nitro, alkyl, alkoxy, haloalkyl, amino, alkylamino, and amido; wherein the concentration of the alkyl dimethyl benzyl quaternary ammonium salt is in a range of between about 1 g/L to about 10 g/L; and a fluorescent agent; wherein the relative concentration of the alkyl dimethyl benzyl quaternary ammonium salt to the concentration of the fluorescent agent in the chemiluminescent composition is between about 1 g/L to about 2 g/L of alkyl dimethyl benzyl quaternary ammonium salt to about 1 mg/L of fluorescent agent.