Patent ID: 8354505

Claim:
A process for obtaining a concentrate of a Factor VIII/Von Willebrand Factor (VWF) complex of human or recombinant origin, said process comprising: a) preparing a solution of a Factor VIII/Von Willebrand Factor complex which contains Von Willebrand Factor in a concentration of up to 12 IU VWF:RCo/ml and has a VWF:RCo/Factor VIII activity ratio of 0.4 to 3, b) a nanofiltration step consisting of filtering the solution prepared in a) through a nanofilter having pore sizes of less than 35 nanometers, at a maximum pressure of less than or equal to 0.5 bar, in the presence of calcium ion at a concentration between 0.05 and 0.14 M, and at a pH greater than 5.5; and thereby obtaining a concentrate of Factor VIII/VWF complex, wherein the VWF:RCo/Factor VIII activity ratio is not affected.