Patent ID: 8105818

Claim:
A method of using an electroporation cuvette containing a frozen, ready-to-use aliquot of electrocompetent cells, comprising: thawing the aliquot of electrocompetent cells within the electroporation cuvette; mixing the thawed cells with a composition comprising a nucleic acid; placing the electroporation cuvette in an electroporation machine; delivering an electrical pulse to the electrocompetent cells, thereby transforming said electrocompetent cells with the nucleic acid, wherein said electroporation cuvette is an electroporation cuvette comprising: first and second electrodes positioned within the electroporation cuvette; and cells frozen within the electroporation cuvette, wherein the electroporation cuvette is configured to permit electroporation of the cells when the cells are thawed, and wherein the cuvette is configured to position said first and second electrodes at a predetermined distance apart during electroporation.