Patent ID: 7442530

Claim:
A process for preparing an L-amino acid selected from the group consisting of L-threonine, L-lysine, and L-valine comprising: a) fermenting a suitable medium with a modified microorganism from the genus Escherichia which produces said L-amino acid, wherein said modified microorganism has been transformed with a vector that over-expresses the iclR gene and/or the fadR gene, compared to the corresponding untransformed Escherichia and wherein L-amino acid production in said modified Escherichia that over-expresses the iclR gene and/or the fadR gene is enhanced compared to the corresponding untransformed Escherichia ; and b) recovering the L-amino acid; wherein the coding sequence of the iclR gene can be isolated from the DNA of an Escherichia by (PCR) and the primers iclR3 (SEQ ID NO: 3) and iclR4 (SEQ ID NO: 4), and wherein the coding sequence of the fadR gene can be isolated from the DNA of Escherichia using polymerase chain reaction (PCR) and the primers fadR3 (SEQ ID NO: 1) and fadR4 (SEQ ID NO: 2).