Patent ID: 7320864

Claim:
A method of detecting a plurality of enzyme activities in a sample, comprising: (a) adding a plurality of types of PNA-substrate chimerae to the sample, said PNA-substrate chimerae having a PNA capture portion and a substrate for an enzyme, wherein different types have different substrates capable of reacting with different enzymes but each type has a particular substrate and a selected PNA sequence capture portion; (b) allowing the enzymes to react with the substrate portions of the chimera population; (c) capturing the reacted PNA-substrate chimerae with different types of oligonucleotides, wherein said different types of oligonucleotides are displayed on differently encoded microparticles, and wherein said capturing is by annealing of a type of oligonucleotide to a selected PNA sequence capture portion; and (d) detecting the changes in the substrates of captured reacted PNA-substrate chimerae; (e) decoding the encoded microparticles to identify which substrates reacted and thereby identify the presence of particular enzymes in the sample.