Patent ID: 7519519

Claim:
A method for predicting a biological activity of a test compound, the method comprising: a) providing a plurality of drug or group signatures, wherein each said signature is associated with a known biological activity and each said signature comprises a plurality of genes and coefficients indicating the degree of change in expression of said genes in response to one or more compound treatments; b) administering a dose of a test compound to a test subject for a selected period of time and obtaining a sample from said test subject; c) from said sample determining an expression response X g c for each gene g of said plurality of genes G s in each of said plurality of drug or group signatures; and d) calculating the Signature Projection Score (SPS) for each of said plurality of drug or group signatures, wherein SPS ⁡ ( c ) = ∑ g ∈ G s ⁢ ( X g c - R g ) ⁢ ( T g - R g ) S g , and wherein, T g is the expression level of gene g of the drug or group signature in response to the one or more compound treatments associated with said drug or group signature, R g is a reference gene expression level for selected gene g, and S g is a scaling factor for the contribution of each gene to the SPS, whereby the drug or group signature resulting in the highest SPS indicates the predicted biological activity of the test compound.