Patent ID: 8178089

Claim:
A method for producing feeder cell free neuroprogenitor cells, said method comprising the steps of: a) Providing a population of adherent primate pluripotent stem cells expressing; Sox2, SSEA-4 and OCT4; b) Contacting said adherent primate pluripotent stem cells with a serum free differentiation medium comprising the components of a minimum essential medium and basic fibroblast growth factor and optionally, non-essential amino acids, leukaemia inhibitory factor (LIF), an antibiotic, mercaptoethanol or another cell medium selected from B27, N2 or mixtures thereof, with an effective amount of at least one pluripotent stem cell differentiation protein to differentiate said adherent primate pluripotent stem cells into neuroprogenitor cells without embryoid body formation or neurosphere transition; and c) Passaging said neuroprogenitor cells.