Patent ID: 8236532

Claim:
A method for obtaining nucleic acid sequence information, said method comprising the steps of: (a) providing a first sequencing reagent to a target nucleic acid in the presence of a polymerase, wherein the first sequencing reagent comprises at least two different nucleotide monomers and no more than three nucleotide monomers, wherein the nucleotide monomers are simultaneously in the presence of the target nucleic acid; (b) providing a second sequencing reagent to said target nucleic acid, wherein said second sequencing reagent comprises one or more nucleotide monomers, at least one of said one or more nucleotide monomers being different from the nucleotide monomers present in said first sequencing reagent, and wherein said second sequencing reagent is provided subsequent to providing said first sequencing reagent; and (c) repeating (a) and (b) for said target nucleic acid, whereby sequence information for at least a portion of said target nucleic acid is obtained, wherein the polymerase extends a polynucleotide strand, further comprising detecting the incorporation of at least one nucleotide monomer into said polynucleotide strand, wherein said detecting comprises detecting pyrophosphate.