Patent ID: 8168746

Claim:
A method for the insertion of protein in mixtures and the recovery of the protein from the mixtures, the method comprising: a) supplying isolated membrane protein; b) mixing the isolated membrane protein with a fluid comprising the saturated phospholipid 1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine, DMPC, the polymer 1,2-Dimyristol-sn-Glycero-3-Phosphoethanolamine N [Methoxy(Polyethylene glycol)-2000] (DMPE), and the co-surfactant N,N-dimethyldodecylamine-N-oxide, LDAO that can exist in a liquid-crystalline gel phase temperatures about 20° C. and above and in a liquid at lower temperatures, so as to form a mixture; c) cycling the mixture between a first temperature and a second temperature; d) diluting the mixture in detergent buffer so as to lower its viscosity; and e) separating the protein from the fluid.