Patent ID: 8383575

Claim:
A complex comprising a Bacillus amyloliquefaciens barnase bound with high affinity to a Bacillus amyloliquefaciens barstar, and further comprising a cobalamin compound which is bound to an N-terminal end or a C-terminal end of said Bacillus amyloliquefaciens barnase or said Bacillus amyloliquefaciens barstar, wherein said Bacillus amyloliquefaciens barnase is (a) a polypeptide which comprises the amino acid sequence set forth in SEQ ID NO: 11; or (b) a polypeptide which comprises at least 98% sequence identity to the amino acid sequence set forth in SEQ ID NO: 11; and said Bacillus amyloliquefaciens barstar is (a) a polypeptide which comprises the amino acid sequence set forth in SEQ ID NO: 12; or (b) a polypeptide which comprises at least 98% sequence identity to the amino acid sequence set forth in SEQ ID NO: 12.