Patent ID: 8685368

Claim:
A method of delivering a biologically active molecule to a cell, the method comprising contacting the cell with a polyplex formed from an interaction between a biologically active molecule and a cellular delivery polymer, wherein: the cellular delivery polymer is a cyclodextrin-based dendritic macromolecule comprising a cyclodextrin core and an oligoamine shell attached to the cyclodextrin core, the cyclodextrin core comprising an alpha, beta, or gamma cyclodextrin and the oligoamine shell comprising polyamine chains depicted by one of the following formulae: wherein n is 1, 2, 3, or 4; or wherein n is 1, 2, 3, or 4; wherein the cyclodextrin-based dendritic macromolecule comprising the polyamine chains depicted by Formula A is formed from a reaction between an alpha, beta, or gamma cyclodextrin substituted with a group depicted by the following formula: and at least one linear polyamine depicted by the following formula: NH 2 â€”Râ€”NH 2 Formula D wherein each R is independently selected from the group consisting of: â€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”, â€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”, â€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”, and â€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 â€”NHâ€”(CH 2 ) 2 ; and wherein the cyclodextrin-based dendritic macromolecule comprising the polyamine chains depicted by Formula B is formed by a reaction between an alpha, beta, or gamma cyclodextrin substituted with an azide group and at least one linear polyamine depicted by the following formula: wherein n is 1, 2, 3, or 4.