Patent ID: 7122343

Claim:
An in vitro method of altering the amount of a DNA repair polypeptide in a cell, comprising: (a) providing a transformed host cell comprising an isolated nucleic acid molecule comprising a nucleic acid segment encoding a vertebrate DNA repair polypeptide having a molecular weight of about 95000 Da as determined by SDS-PAGE, operably linked to a promoter functional in the host cell, wherein the DNA repair polypeptide is associated with the Mre11/Rad50 complex, wherein the nucleic acid segment comprises SEQ ID NO:1 or encodes SEQ ID NO:2; and (b) expressing the nucleic acid molecule in the transformed host cell as recombinant DNA repair polypeptide, wherein the amount of the recombinant polypeptide produced by the transformed cell is different than the amount of the DNA repair polypeptide produced by a corresponding untransformed cell.