Patent ID: 6849710

Claim:
A process for the synthesis of a synthetic polypeptide analog of parathyroid hormone (PTH) or parathyroid hormone related peptide (PTHrP), or salt thereof, in which amino acid residues (22-31) form an amphipathic α-helix, said residues (22-31) selected from the group consisting of (SEQ ID NOS: 85, 86, 26, 27, 28, 29, and 30), which process comprises: a) independently synthesizing precursor peptide fragments of the desired polypeptide on a solid resin support; b) cleaving all but the intended ultimate C-terminal precursor peptide fragment of the desired polypeptide from their respective resin supports; c) sequentially condensing said cleaved precursor peptide fragments with the resin bound C-terminal peptide fragment to form the desired polypeptide product; d) removing side chain protecting groups; and e) cleaving the polypeptide product from the resin support, wherein at least two of the fragments are coupled via leucine-leucine bonding.