Patent ID: 8557746

Claim:
A method for the high throughput screening for agents that inhibit the multidrug resistance pathway in fungi comprising: providing a solution of fluorescein-labeled Pdr1p activation domain, a Gal11p KIX domain and a test agent; and comparing the fluorescence polarization of the solution of fluorescein-labeled Pdr1p activation domain, the Gal11p KIX domain and the test agent, with the fluorescence polarization of a solution of fluorescein-labeled Pdr1p activation domain and a Gal11p KIX domain; wherein the fluorescein-labeled Pdr1p activation domain consists of fluorescein and 30 amino acids of the CgPdr1p activation domain, having the amino acid sequence LGTLDEFVNKGDLNELYNSLWGDLFSDVYL. (SEQ ID NO: 14)