Patent ID: 8759054

Claim:
Gold nanoparticles on a sharp edge support and having DNA loaded thereon, said nanoparticles being made by a process comprising: [a] inoculating a microbial culture capable of synthesizing intracellular gold nanoparticles in a nutrient medium and culturing to obtain a biomass; [b] harvesting the biomass obtained in step [a] followed by washing the biomass with sterilized distilled water under aseptic conditions; [c] suspending the washed biomass obtained in step [b] in HAuCl 4 solution and incubating for 2-3 days followed by washing the biomass with sterilized distilled water under aseptic conditions; [d] heat treating the washed biomass obtained in step [c] for 6-8 hours in a tubular furnace under inert conditions to obtain the heat treated carbon gold HTC-600 Au; [e] washing the HTC-600 Au as obtained in step [d] with ethanol; [f] mixing the ethanol-washed, sterilized HTC-Au-600 with DNA suspended in the XHO buffer (Tris.Cl, NaC-based buffer); [g] equilibrating the mixture as obtained in step [f] with spermidine and PEG in sequence followed by sonication in 2.5 M CaCl 2 ; [h] centrifuging the sonicated mixture as obtained in step [g] at 12000 rpm and washing the pellets twice using absolute alcohol and finally resuspending in the required volume of ethanol to obtain the DNA loaded supported gold nanoparticles.