Patent ID: 8179599

Claim:
An optical system comprising an optical unit with an optical axis extending through a light transmissive sample embedded in a transparent substrate to focus on the sample embedded in the transparent substrate and to scan the sample according to a main plane of the transparent substrate, the transparent substrate having a refractive index, a surface and the main plane, and a substantially wedge shaped transparent correction region arranged in the optical axis between the optical unit and the transparent substrate, the correction region having a first plane, a second plane, and a refraction index, the correction region having a cross-section in the form of a triangle, and being movable relative to the transparent substrate, on the surface of the transparent substrate; wherein the optical axis extends with an angle unequal to zero relative to the normal of the main plane of the transparent substrate, and wherein the refraction index of the correction region is substantially equal to the refraction index of the transparent substrate, the first plane of the correction region is arranged parallel to the main plane of the transparent substrate and the second plane of the correction region is arranged perpendicular to the optical axis.