Patent ID: 8329632

Claim:
A detergent composition comprising a variant of a subtilisin KL in combination with at least one other enzyme selected from the group consisting of protease, lipase, cutinase, amylase, carbohydrase, cellulase, pectinase, pectate lyase, hemicellulase, oxidase, or a combination thereof, and wherein the variant of a subtilisin KL further comprises a mutation: *36D; P14T; N18K; N62D; V83L; A133P; E136Q; E136R; E136K; N140R; N140K; S141E; S141N; S141Y; S141R; T143R; T143K; S153R; S156R; A160R; S162R; S162K; I165R; I165K; Y171R; Y171K; A172R; A172K; A174R; N173R; N173K; A174K; N76D; Y176R; Y176K; A187R; A187K; S188P; S190P; Q191R; Y192R; Y192R; Q191P; Y192A; Y192P; D197N; D197R; D197E; D197K; D197G; A228V; A230V; T260R; T260K; G264R; G264K; S265T; S265R; S265K; N218S; M222S; M222A; M222G; M222T; M222V; M222S; N243R; V244R; N248R; K251R; N252R; N261R; S9R + A15T + T22A + N218S + K251R; S9R + A15T + T22A + V84I + N218S; V30I + V139L + N218S; V84I + V139L + N218S; N76D + N218S; N76D + A228V; N76D + A230V; N76D + N218S + A230V; N76D + A228V + A230V; N218S + R247Q; N218S + R247H; N218S + R247E; N218S + R247K; D181N + N218S; N218S + A230V; K251R + S265K; P14T + N18K; T274H + R275H + *275aH + *275bH + *275cH + *275dH; T274H + R275H + *275aH + *275bH + *275cH; S87N + S101G, V104N; *36D + N76D + H120D + G195E + K235L; A133P + M222S; *96aA; *96aA + A98T; *96aA + A133P; *96aA + A98T + A133P; *96aA + A98T + N218S; *97aP + A98T + N218S; *98aT; *98aT + S99N + N218S; G97D + *98aT + N218S; *99aE; *99aD; *99aD + M222S; N76D + s99A + *99aE; N76D + *99aD + A230V; S99A + *99aD; S99A + *99aD + M222S; S99A + *99aD + N218S; S99A + *99aE + A230V; A228V + A230V; or *130aL + P194A.