Patent ID: 7323140

Claim:
A microfluidic device, comprising: a microfluidic network, wherein the microfluidic network comprises several modules, including: an enrichment module configured to accept a particle-containing fluid sample, and to create an enriched sample therefrom; a microdroplet preparation module configured to receive the enriched sample and to create a microdroplet therefrom; a cell lysing module configured to lyse the microdroplet of enriched sample; a mixing module configured to mix one or more reagents and the enriched sample; and a DNA manipulation module configured to receive the enriched sample mixed with one or more reagents; wherein the several modules are operatively connected to one another by one or more channels; and wherein at least one of the several modules is isolatable by a valve; and wherein at least one actuator is configured to move a microdroplet of fluid from one of the several modules to another of the several modules.