Patent ID: 7914689

Claim:
A method of enriching a scaffold for application relative to an anatomy, comprising: connecting a first piston and a second piston with a connection member; obtaining a volume of a first whole material; placing at least a portion of the obtained volume of the first whole material into a container: forming a first fraction of the first whole material by centrifuging the placed portion of the obtained volume of the first whole material placed in the container; disposing the first piston relative to the second piston; obtaining a volume of a second whole material that is different from the first whole material; placing at least a portion of the obtained volume of the second whole material into the container: forming a second fraction of the second whole material by centrifuging the placed portion of the obtained volume of the second whole material placed in the container; containing at least a portion of both the first fraction and the second fraction near a first collection surface of the first piston; and applying a selected portion of the first fraction and the second fraction to the scaffold.