Patent ID: 7858302

Claim:
A process for incorporating a desirable nucleic acid sequence into an analyte or a library of analytes, said process comprising the steps of: a) providing (i) said analyte or library of analytes; (ii) a chimeric nucleic acid comprising at least two segments, the first segment comprising five or more universal bases and the second segment comprising a template for synthesizing said desirable nucleic acid sequence wherein said chimeric nucleic acid is incapable of strand extension; and (iii) reagents for carrying out hybridization and template-dependent strand extension; b) binding or hybridizing said chimeric nucleic acid (ii) to said analyte or library of analytes (i); and c) extending the 3′ end of said analyte or library of analytes; wherein said second segment is a template for said extending step (c) when said second segment is in proximity to the 3′ end of said analyte or library of analytes.