Patent ID: 7244598

Claim:
A method for detecting modification of MEK proteins or MAPK proteins of a Raf/MEK/MAPK pathway, comprising the steps of: a) placing a polymer gel contact mask having holes on a substrate, the holes together with the portions of the substrate which overlie the holes forming cavities; b) immobilizing inactive MEK proteins and inactive MAPK proteins on areas of the substrate underlying the holes of the polymer gel contact mask that have the size and orientation of wells of a 96 well, 384 well, 1536 well, or 3456 well microwell plate; c) exposing the inactive MEK proteins and inactive MAPK proteins to a solution of active Raf proteins, active MEK proteins, ATP, and potential inhibitors of at least one of the active Raf proteins or the active MEK proteins; d) allowing binding of the active Raf proteins and the active MEK proteins to the inactive MEK proteins and the inactive MAPK proteins; and e) detecting modification of the inactive MEK proteins or the inactive MAPK proteins of the Raf/MEK/MAPK pathway.