Patent ID: 8129147

Claim:
A modified Neisseria meningitidis fastidious culture medium (NMFM) for producing a higher amount of capsular polysaccharides and a lesser amount of cellular biomass from Neisseria meningitidis comprising: DI (deionized) water, NaCl, K 2 HPO 4 , NH 4 Cl, K 2 SO 4 , MgCl 2 .6H 2 O, CaCl 2 .2H 2 O, Glucose, L-Glutamic Acid, L-Arginine, Glycine, L-Serine, L-Cysteine.HCl, Fe (111) Citrate, Yeast Extract (30 k dia-filtered), and antifoam, with the pH of the composition maintained from 6.5 to 7.0 for serogroups A, C, Y and W-135 and characterized in that the use of the medium yields a higher percentage of polysaccharides and a lower percentage of cellular biomass as compared to Catlin or Watson-Scherp medium.