Patent ID: 8361759

Claim:
A process for recovering and concentrating the farnesylated dibenzodiazepinone of structural Formula I comprising: a) fermenting a strain of a microorganism that produces the farnesylated dibenzodiazepinone of Formula I in an aqueous culture medium, thereby producing a fermentation broth comprising said farnesylated dibenzodiazepinone, wherein the bacterium is Micromonospora sp. [S01]046 having IDAC accession number 231203-01 or Micromonospora sp. [S01U02]046 having IDAC accession number 070905-01; b) adjusting said fermentation broth by acidification to a pH value of between about 2 to 4 to allow said farnesylated dibenzodiazepinone to associate with a particulate matter present in said broth; c) harvesting said particulate matter from said broth, thereby obtaining a harvested particulate matter; d) extracting said harvested particulate matter with a volume of an organic solvent in a proportion of about 2:1 to about 5:1 to said harvested particulate matter, thereby forming an extract; and e) subjecting said extract to evaporation or water displacement, thereby forming a first concentrate comprising said farnesylated dibenzodiazepinone of Formula I, wherein said first concentrate comprises said farnesylated dibenzodiazepinone at a concentration greater than about 50-fold than said farnesylated dibenzodiazepinone in said fermentation broth of a), and wherein at least about 50% of the amount of said farnesylated dibenzodiazepinone in said fermentation broth of a) is recovered in said first concentrate.