Patent ID: 8703486

Claim:
An in vitro method for producing a human polyclonal IgG preparation, said method comprising: a) obtaining an initial human polyclonal B-cell population; b) prior to culture, substantially depleting IgM-secreting cells and IgD-secreting cells from the initial human polyclonal B-cell population so as to obtain a human polyclonal B-cell population enriched in IgG-secreting cells; c) placing the human polyclonal B-cell population enriched in IgG secreting cells in a culture medium; and d) culturing the human polyclonal B cell population enriched in IgG-secreting cells under conditions enabling the production of the human polyclonal IgG preparation from the polyclonal B cell population; wherein the human polyclonal B cell population of step b) has a concentration of IgM-secreting cells of less than 5% and a concentration of IgD-secreting cells of less than 5%.