Patent ID: 7794995

Claim:
A purified PKB Ser 473 kinase complex, which has PKB Ser 473 kinase activity and an apparent molecular weight of 450-650 kDa when fractionated by gel filtration chromatography, wherein the purified PKB Ser 473 kinase complex has been isolated from a cell-free extract that has measurable PKB Ser 473 kinase activity in 0.2 μg of protein when detected in a kinase assay in which a PKB peptide substrate is phosphorylated with 32 P labeled phosphate, wherein the purified PKB Ser 473 kinase complex elutes with the apparent molecular weight of 450-650 kDa when fractionated by the gel filtration chromatography and the measurable PKB Ser 473 kinase activity in the cell-free extract is at least 2000 times greater than a PKB Ser 473 kinase activity in a crude cell extract, wherein the kinase activities are measured using the kinase assay.