Patent ID: 8761857

Claim:
A method of sensing an analyte in a derivative portion of a biofluid, comprising: passing a biofluid through an interface to produce a derivative portion of the biofluid, wherein the interface further comprises an organic-metal catalytic agent disposed only in the interface and sufficient to catalyze the degradation of at least one of a reactive oxygen species and a reactive nitrogen species of biological origin in at least one of the biofluid and the derivative portion of the biofluid, the agent selected from a proteinaceous catalytic agent, a non-proteinaceous catalytic agent, or any combination thereof; contacting the derivative portion of the biofluid with a transducer sufficient for generating a signal that corresponds to a concentration of an analyte, wherein the transducer comprises a sensing layer having an analyte responsive enzyme; and generating a signal that corresponds to a concentration of the analyte in the derivative portion of the biofluid.