Patent ID: 8603967

Claim:
A method for transferring a foreign substance of interest from outside a eukaryotic cell at least into the cytoplasm of the cell, comprising the steps of: (a) preparing a construct comprising: a carrier peptide fragment consisting of the amino acid sequence of SEQ ID NO: 1; and a foreign substance of interest bonding directly or indirectly via a linker to the N-terminus and/or C-terminus of the carrier peptide fragment; (b) adding the construct containing the carrier peptide fragment to a culture medium that includes the eukaryotic cell or a tissue containing the cell; (c) culturing the eukaryotic cell or the tissue containing the cell in the medium containing the construct; and (d) transferring the construct into the culturing eukaryotic cell from outside of the cell by cell membrane permeability of the carrier peptide fragment itself.