Patent ID: 8497068

Claim:
A method for detecting hybridization between a probe polynucleotide and a target polynucleotide using a microarray, comprising the steps of: 1) bringing a fluorescence-labeled target polynucleotide and a fluorescence-labeled marker polynucleotide capable of hybridizing to a reference polynucleotide into contact with a microarray having reference spots at one or more positions at which the reference polynucleotide is immobilized, and a plurality of detection spots at which the probe polynucleotide is immobilized; 2) washing the microarray to remove unreacted marker and target polynucleotides; 3) measuring fluorescence at the reference spots and determining that the measured value meets a predetermined level; and 4) measuring fluorescence at each detection spot; wherein, in the microarray, the detection spots and the reference spots are aligned, the reference spots are present at two or more positions, and the position of a detection spot is determined based on the distance of the detection spot from the reference spots and the angles between a line connecting each detection spot and a reference spot and the reference line, wherein the reference line is a line connecting two reference spots.