Patent ID: 8628914

Claim:
A method for measuring helicase activity comprising: a. providing a nucleic acid duplex comprising a first immobilizable nucleic acid strand and a labeled second strand; b. immobilizing the nucleic acid duplex; c. contacting the immobilized nucleic acid substrate duplex with a helicase to form a helicase-duplex mixture; d. incubating the helicase-duplex mixture with a trap oligonucleotide under conditions for helicase activity, wherein the trap oligonucleotide binds to the labeled second strand; e. separating non-immobilized nucleic acids, wherein the non-immobilized nucleic acids comprise the trap oligonucleotide bound to labeled second strand; and f. detecting the label present with the immobilized nucleic acids, wherein a decrease in the amount of label present indicates helicase activity.