Patent ID: 7585970

Claim:
A method for synthesizing a solid-support-attached oligonucleotide or polynucleotide, the method comprising: (a) providing a functionalized support having the structure (Id) wherein the groups are defined as follows: is a solid support, Trl- is the triaryl methyl linker group having three aryl groups, wherein each of the three aryl groups are bound to a central methyl carbon, and at least one of said three aryl groups has one or more substituents, wherein one of said substituents is bound to the solid support either directly or through Lnk′ and the central methyl carbon is bound to the 5′-hydroxyl of (Ntd) k either directly or through Lnk, Lnk′- is a linking group linking the solid support and the triaryl methyl linker group, or is a bond linking the solid support and the triaryl methyl linker group, (Ntd) k - is a polynucleotide moiety having k nucleotide sub-units, wherein k is an integer in the range from zero to about 200, Lnk- is a linking group linking the triaryl methyl linker group and the polynucleotide moiety, or is a bond linking the triaryl methyl linker group and the polynucleotide moiety, and Nucl- is the nucleoside moiety having a reactive site hydroxyl; (b) contacting said functionalized support with a precursor having the structure (IVd) wherein the groups are defined as follows: O and H represent oxygen and hydrogen, respectively R 1 is hydrido, hydroxyl, protected hydroxyl, lower alkyl, substituted lower alkyl or alkoxy, one of R 2 or R 3 is a hydroxyl protecting group; and the other of R 2 or R 3 is a phosphorus derivative capable of reacting with a reactive site hydroxyl of a nucleoside moiety to produce either a phosphite triester or a phosphite diester internucleotide linking moiety, and Base is a heterocyclic base, under conditions and for a time sufficient to result in phosphite triester internucleotide or a phosphite diester linking moiety formation; (c) contacting the result of step (b) with a reagent comprising a nucleophilic ion or a salt thereof that exhibits an alpha effect at neutral to mildly basic pH to concurrently remove the hydroxyl protecting group from the result of step (b) and oxidize the internucleotide linking moiety wherein said reagent comprises a peroxide.