Patent ID: 8293487

Claim:
An improved method of immunodetection process of an antigen of interest in a sample, wherein the process comprises the steps of: a) applying said sample containing said antigen to bind said antigen onto a solid phase; b) blocking said solid phase to eliminate non-specific antibody binding to said solid phase; c) incubating said solid phase with a primary antibody that is specific for said antigen, and which is labeled, directly or indirectly through a secondary antibody, with reporter enzymes, to form an antigen-antibody immunocomplex; d) eluting said solid phase with a solution containing excess of free said antigen or any polypeptide, which is capable of disrupting said antigen-antibody immunocomplex through competition, in order to liberate said primary antibody, directly or indirectly labeled with reporter enzymes, from said immunocomplex; e) collecting the eluates and quantifying said bound antigen of step a) by quantifying said liberated primary antibody in a reporter assay.