Patent ID: 8298789

Claim:
A process for synthesis of rhPTH (1-34), said process comprising: i) constructing a cDNA coding chimeric nucleotide ORF as set forth in SEQ. ID NO.: 1 by amplification of the cDNA by RT-PCR using gene specific primers SEQ. ID NO.: 3, SEQ. ID NO.: 4, SEQ. ID NO.: 5, SEQ. ID NO.: 6, SEQ. ID NO.: 7 and SEQ. ID NO.: 8 encoding rhPTH (1-34) chimeric fusion protein as set forth in SEQ. ID NO.: 2, ii) expressing the chimeric fusion protein in the presence of lactose as inducer in the range of about 10-30% of total protein, iii) purifying the rhPTH (1-34) obtained by an orthogonal process wherein the purification is carried out by cation exchange chromatography to a purity of >98% optionally followed by hydrophobic interaction chromatography (HIC) or RP-HPLC to a purity of ≧99%.