Patent ID: 8314052

Claim:
A method for simultaneously selecting a plurality of aptamers for multiple different targets comprising: contacting a library containing aptamers comprising a diversity of nucleic acids each having a randomized region and a constant region, with a plurality of different targets simultaneously in a single reaction volume, each of said targets being fixed at a different predetermined location on an array; partitioning members of said library that do not bind to said plurality of different targets; marking the members of the library that bind to the plurality of different targets with identifiers, each of said identifiers being indicative of the particular target; applying a different identifier to each of said different predetermined locations on said array, said identifier comprising an single-stranded DNA oligonucleotide having a variable region which identifies one of said predetermined locations on said array, said variable region being flanked by a first constant region having a sequence for hybridizing to said constant region of said library, and a second constant region having a primer binding site; identifying the binding members of the library and correlating them to the targets via said identifiers; and performing a nucleic acid amplification reaction on each of said hybridized identifiers and said aptamers to generate an amplification product; wherein said amplification product correlates the sequence of one of said aptamers to one of said targets to which said aptamer binds.