Patent ID: 7244614

Claim:
A recombinant nucleic acid molecule encoding a recombinant fusion protein for detecting binding of a molecule of interest, comprising the following operably linked regions in frame relative to each other: a) a first nucleic acid sequence encoding a detection domain; b) a second nucleic acid sequence encoding a first localization domain; and c) a third nucleic acid sequence encoding a binding domain for the molecule of interest, wherein the binding domain is one or both of a covalent binding domain and a non-covalent binding domain; wherein the third nucleic acid sequence encoding the non-covalent binding domain, when present, is separated from the second nucleic acid sequence by 0–60 nucleotides, and wherein the third nucleic acid sequence encoding the covalent binding domain, when present, is separated from the second nucleic acid sequence by 0–12 nucleotides, wherein the third nucleic acid sequence encodes a binding domain consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43, SEQ ID NO:45, SEQ ID NO:47, SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:53, SEQ ID NO:55, SEQ ID NO:57, SEQ ID NO:59, SEQ ID NO:61, SEQ ID NO:63, SEQ ID NO:65, SEQ ID NO:67, SEQ ID NO:69, SEQ ID NO:71, SEQ ID NO:73, SEQ ID NO:75, SEQ ID NO:77, SEQ ID NO:79, SEQ ID NO:81, SEQ ID NO:83, SEQ ID NO:85, SEQ ID NO:87, SEQ ID NO:89, SEQ ID NO:91, SEQ ID NO:93, SEQ ID NO:95, SEQ ID NO:97, SEQ ID NO:99, SEQ ID NO:101, SEQ ID NO:103, SEQ ID NO:105, SEQ ID NO:107, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NO:113, and SEQ ID NO:115, SEQ ID NO:117, SEQ ID NO:119, SEQ ID NO:120, SEQ ID NO:122, SEQ ID NO:124, SEQ ID NO:126, SEQ ID NO:128, SEQ ID NO:130, SEQ ID NO:132, SEQ ID NO:134, SEQ ID NO:136, SEQ ID NO:138, SEQ ID NO:140, SEQ ID NO:141, SEQ ID NO:143, SEQ ID NO:310, SEQ ID NO:311, SEQ ID NO:312, SEQ ID NO:313, SEQ ID NO:314, SEQ ID NO:336, SEQ ID NO:337, SEQ ID NO:338, SEQ ID NO:339, SEQ ID NO:341, SEQ ID NO:343, SEQ ID NO:345, SEQ ID NO:347, SEQ ID NO:349, SEQ ID NO:350, SEQ ID NO:352, SEQ ID NO:354, SEQ ID NO:356, SEQ ID NO:358, SEQ ID NO:360, SEQ ID NO:362, SEQ ID NO:364, and SEQ ID NO:366; and wherein the second nucleic acid sequence and the third nucleic acid sequence do not both occur in a single non-recombinant nucleic acid molecule, or do not both occur in a single non-recombinant nucleic acid molecule with the same spacing as in the recombinant nucleic acid molecule encoding a recombinant fusion protein for detecting binding of a molecule of interest.