Patent ID: 7867755

Claim:
A method for detecting plural different proteins in a protein mixture, comprising: (1) denaturing and fragmenting said proteins to produce linear peptide fragments comprising peptide epitopes; (2) providing an array comprising, at known positions on the array, first capture molecules produced using synthetic peptides and being specific for said individual peptide epitopes, whereby the positions of the first capture molecules produce a spatially resolved pattern for identifying the linear peptide fragments and thus proteins that correspond to the linear peptide fragments; (3) incubating the array with the linear peptide fragments under conditions whereby the first capture molecules specifically bind to and thereby form complexes with the linear peptide fragments; and (4) detecting the linear peptide fragments, if any, bound to the first capture molecules by contacting said complexes with labeled second capture molecules selected from the group consisting of antibodies, antibody fragments, and peptide aptamers.