Patent ID: 7074398

Claim:
A method for producing a recombinant retroviral particle encoding an SDI-1 polypeptide or a fragment thereof, wherein the SDI-1 polypeptide or fragment thereof inhibits cell proliferation, the method comprising: (a) stably transfecting an isolated producer cell line with a retroviral vector comprising in 5â€² to 3â€² order: (1) a 5â€² LTR; (2) a nucleic acid sequence encoding an SDI-1 polypeptide or fragment thereof, wherein said SDI-1 polypeptide or fragment thereof inhibits cell prolfieration; and (3) a 3â€² LTR region comprising a complete or partial U3 deletion and an insertion in place thereof, wherein said insertion comprises a polylinker sequence into which a regulatory element or a promoter has been cloned; and (b) producing said recombinant retroviral particle in said stably transfected isolated producer cell line, wherein said retroviral particle encodes said SDI-1 polypeptide or fragment thereof, and further wherein said regulatory element or promoter becomes operatively linked to said nucleic acid sequence and regulates expression of said nucleic acid sequence upon infection of a target cell by said recombinant retroviral particle.