Patent ID: 7491527

Claim:
An integrated cartridge to perform differential lysis, the integrated cartridge comprising: a. a sample input chamber including an external access port configured to receive a sample from outside the integrated cartridge, the sample having at least two different cell types, wherein the sample input chamber includes a mounting seat configured to be removably coupled to a sonication device, further wherein the sample input chamber is configured to selectively lyse a first cell type while leaving intact at least a remaining second cell type within the sample, thereby forming a first lysate; b. separating means to separate the first lysate from the sample; c. a collection vessel to collect the first lysate; and d. microfluidic circuitry to couple the sample input chamber, the separating means, and the collection vessel, wherein the microfluidic circuitry is configured to direct the sample including the intact second cell type to the sample input chamber, further wherein the sample input chamber is configured to lyse the second cell type within the sample.