Patent ID: 8163706

Claim:
An extract of Centella asiatica having more than 95 wt % of a mixture comprising madecassoside and terminoloside relative to the total weight of the extract prepared by a process comprising the steps of: a) creating an extract by exposing the parts of Centella asiatica that are above ground to an alcoholic solvent to obtain an alcoholic solution; b) passing the alcoholic solution obtained in step a), over an anionic resin to obtain an aqueous-alcoholic eluate; c) selectively defatting the aqueous-alcoholic eluate obtained in step b) by liquid/liquid extraction to obtain a defatted aqueous-alcoholic phase; d) concentrating the defatted aqueous-alcoholic phase obtained in step c) using successive filtration to obtain an aqueous phase; e) passing the aqueous phase obtained in step d), over a cationic resin and then over an anionic resin; f) stabilizing the aqueous phase obtained in step e) by adding alcohol to obtain a prepurified aqueous-alcoholic phase; g) separating the prepurified aqueous-alcoholic phase obtained in step f) using a chromatographic column having a stationary phase and a mobile phase; and h) obtaining a mixture comprising more than 95 wt % of madecassoside and terminoloside relative to the total weight of the extract.