Patent ID: 8765378

Claim:
A method for determination of thymidine kinase (TK) activity in a biological sample comprising the steps of: contacting, in a container containing a single stranded polynucleotide as a primer or template attached to a solid surface, a Basic Reaction Mixture, in a buffer, comprising the following components: a single stranded polynucleotide as a template and/or primer not attached to a substrate, a modified deoxy nucleoside as a kinase enzyme substrate, a phosphate donor, a nucleotide polymerizing enzyme, and a kinase enzyme source devoid of TK activity, with the biological sample, incubating the mixture, optionally washing the incubated mixture to remove non-attached reaction components, adding to the incubated mixture a labeled affinity molecule with affinity for the modified deoxy nucleoside; determining the amount of modified deoxy nucleoside that has been incorporated into the solid surface-attached primer or template by determining the amount of attached labelled affinity molecule, and determining the TK activity present in the biological sample from the amount of incorporated modified deoxy nucleoside.