Patent ID: 6881540

Claim:
A method for extra-column, high throughput, parallel screening of a plurality of displacer candidates for efficacy in separating a bioproduct from one or more impurities by means of a displacement chromatography system comprising a mobile phase solvent and a stationary phase resin, said method comprising: determining the equilibrium concentration of the bioproduct in at least one first extra-column batch consisting of the bioproduct, the mobile phase solvent, the stationary phase resin, and, optionally, the one or more impurities; determining the equilibrium concentration of the bioproduct in the mobile phase solvent in a plurality of second extra-column, high throughput, parallel screening batches, each comprising a displacer candidate, the bioproduct, the mobile phase solvent, and the stationary phase resin; wherein the amount of the bioproduct displaced from the stationary phase resin in each of the plurality of second batches, relative to the total amount of the bioproduct bound in the first batch, is an indicator of the efficacy of the displacer contained therein.