Patent ID: 7988932

Claim:
A method for determining the percentage of hemoglobin that is HbA1c, the method comprising: providing a disposable strip comprising a first solid substrate comprising a sample well, a reference well, and a reaction well, wherein the wells are in fluidic communication via a first capillary channel, and wherein the reference well comprises a first lysing agent and the reaction well comprises a second lysing agent, a HbA1c specific antibody, and a stir bar, a second solid substrate comprising holes wherein the first solid substrate and the second solid substrate are joined and the holes communicate with the wells, and a membrane having a capture zone wherein the membrane is connected to the reaction well via a second capillary channel; placing a sample in the sample well; adding a diluent to the sample well; determining the total hemoglobin from the reference well and the total HbA1c from the capture zone; and dividing the total HbA1c by total hemoglobin to obtain the percentage of hemoglobin that is HbA1c.