Patent ID: 6970729

Claim:
Method for determining by imaging techniques the local distribution of first and second independent measurement variables measured relative to or present in a predefined measurement area of a biological sample comprising the steps of: applying a single sensor film to the predefined measurement area of the biological sample, said single sensor film containing a luminescence indicator which reacts to said first independent measurement variable by a change in luminescence decay time, directing first excitation radiation into said single sensor film to excite said luminescence indicator and cause said luminescence indicator to emit first measurement radiation, detecting said first measurement radiation and determining therefrom local distribution of said first independent measurement variable in a first optical measuring process, directing second excitation radiation into and through said single sensor film and into said biological sample to cause second measurement radiation to be emitted from said biological sample, and detecting said second measurement radiation and determining therefrom local distribution of said second independent measurement variable in a second optical measuring process.