Patent ID: 6838257

Claim:
A method for producing a variant of a parent pullulanase, wherein the parent pullulanase has more than 50% homology with the amino acid sequence shown in SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO:6, and wherein the variant has at least one altered property as compared to the parent pullulanase, the method comprising: a) modeling the parent pullulanase on the three-dimensional structure of SEQ ID NO:2 depicted in Appendix 1 to produce a three-dimensional structure of the parent pullulanase; b) identifying in the three-dimensional structure obtained in step (a) at least one structural part of the parent pullulanase, wherein an alteration in said structural part is predicted to result in an altered property; c) modifying the nucleic acid sequence encoding the parent pullulanase to produce a nucleic add sequence encoding a variant of the parent pullulanase having a deletion, insertion, or substitution of one or more amino acids at a position corresponding to said structural part; and d) expressing the modified nucleic acid sequence in a host cell to produce the variant pullulanase.