Patent ID: 8669091

Claim:
A bacterium comprising the following components: (I) at least one polynucleotide encoding a heterologous antigenic determinant that induces a CTL response against a tumor cell; (II) at least one polynucleotide encoding a heterologous protein toxin or protein toxin subunit; and (III) (a) at least one polynucleotide encoding a transport system that expresses the products of (I) and (II) on the outer surface of the bacterium or that secretes products of (I) and (II) from the bacterium; and optionally, (b) at least one polynucleotide that encodes a protein that lyses the bacterium when it is inside of the cytosol of a mammalian cell thereby releasing plasmids or expression vectors contained in the lysed bacterium; and (IV) at least one polynucleotide that activates the expression of one or more of (I), (II) and/or (III) in the bacterium; wherein said bacterium is selected from the group consisting of Escherichia, Salmonella, Yersinia, Vibrio, Listeria, Shigella, Yersinia , and Pseudomonas , with the proviso that it is not Vibrio cholera ; and wherein polynucleotides (I), (II), (III) and (IV) are different from each other and polynucleotides (I), (II) and (III) encode proteins that are different from each other.