Patent ID: 8236184

Claim:
A method of separating a composite liquid into at least a first component and a second component wherein the second component comprises platelets and the first component comprises plasma, comprising: centrifuging a separation bag containing a volume of composite liquid so as to cause the sedimentation of at least the first component and the second component; squeezing some of the first component from the separation bag to a second transfer bag; squeezing the separation bag to transfer some of the second component, after the squeezing step to squeeze some of the first component, to a first transfer line attached to a first transfer bag and the separation bag; stopping the transfer of the second component to the first transfer line; squeezing the separation bag after the stopping step to build up an over-pressure in the separation bag; and transferring, using the over-pressure, a part of the second component remaining in the separation vessel to the transfer bag through the first transfer line wherein the over-pressure causes the second component to rapidly move toward the first transfer bag and to clean the first transfer line of any residual second component from the prior squeezing step to transfer the second component.