Patent ID: 8361770

Claim:
A composition comprising a polynucleotide that encodes a polypeptide comprising an amino acid sequence set forth in SEQ ID NO: 4, 6, 8 or 10, or an amino acid sequence that is a conservative variant of SEQ ID NO: 2, which conservative variant is at least 90% identical to SEQ ID NO: 2 and comprises two or more amino acids selected from the group consisting of: leucine at a position corresponding to Tyr32; proline at a position corresponding to Leu65; glutamic acid at a position corresponding to Gln155; glycine at a position corresponding to Asp158; cysteine or threonine at a position corresponding to Ile159, and lysine at a position corresponding to Leu162; wherein the polypeptide aminoacylates a cognate orthogonal tRNA (O-tRNA) with a sulfotyrosine.