Patent ID: 8540868

Claim:
A method of evaluating responsiveness of a subject to a therapeutic regimen, the method comprising: assaying at least one biological sample obtained from the subject for a nucleotide sequence of interest that is associated with responsiveness to the therapeutic regimen, comprising: providing a sample analyzer comprising a plurality of chemical-sensitive field effect transistors (chemFETs), the chemFETs having a gate oxide, a floating gate comprising conductors formed in a plurality of conductor layers and electrically coupled to one another, and a passivation layer overlying the floating gate, the sample analyzer further comprising signal lines for the sensors within at least one of the conductor layers; disposing at least one interrogating nucleic acid in proximity to or in contact with at least a portion of the chemFETs wherein the at least one interrogating nucleic acid is at least partially complimentary to the at least one target nucleic acid of interest; disposing at least one target nucleic acid of interest associated with the biological sample in proximity to or in contact with at least a portion of the chemFETs wherein interactions between the at least one interrogating nucleic acid and the at least one target nucleic acid effectuate changes in at least one electrical property of at least a portion of the chemFETs; and identifying hybridization interactions between the at least one interrogating nucleic acid and the at least one target nucleic acid by detecting the changes in electrical properties of at least a portion of the chemFETs; and generating an evaluation of responsiveness of the subject to the therapeutic agent on the basis of the hybridization interactions for the at least one biological sample.