Patent ID: 7029881

Claim:
A method for immobilizing a cDNA library on an original support and at least one replica support comprising: a. immobilizing an amino group on the surface of the original support b. contacting the original support with oligo (dT)n to immobilize the oligo (dT)n onto the original support, wherein n is from 15 to 30; c. treating the original support with a reaction solution comprising total RNA, reverse transcriptase, and nucleotides to construct cDNA from mRNA; d. maintaining the original support in contact with the reaction solution at 20° C. to hybridize oligo (dT)n on the original support and mRNA in the total RNA solution; e. treating the original support with reverse transcriptase solution and maintaining the original support in contact with reverse transcriptase to produce a cDNA library immobilized on the original support; f. cleaning the original support and heating the original support to dehybridize mRNA from the original support; and g. repeating steps b–f using a replica support in place of the original support in order to produce a replica cDNA library immobilized on the support.