Patent ID: 8183433

Claim:
A method of controlling a genetically-modified plant or plant cells, said method comprising the following steps: (a) providing a genetically-modified plant or plant cells, said plant or plant cells containing a heterologous nucleic acid encoding a first polypeptide containing or consisting of a first fragment of a protein, said plant or plant cells further containing an additional heterologous nucleic acid that is controlled by an enzymatic activity of said protein; and (b) introducing a second polypeptide into cells of said genetically-modified plant or plant cells by Agrobacterium expressibly encoding said second polypeptide, said second polypeptide not being encoded in T-DNA of a Ti-plasmid of said Agrobacterium , said second polypeptide containing (i) a second fragment of said protein and (ii) a virE2 or virF peptide sequence enabling the introduction of said second polypeptide into cells of said genetically-modified plant or plant cells, whereby said first fragment and said second fragment jointly generate, only when jointly present, an enzymatic activity of said protein by intein-based trans-splicing, said enzymatic activity being the activity of a site-specific recombinase or integrase; said enzymatic activity triggering formation of a DNA, an RNA or a protein of interest from said additional heterologous nucleic acid.