Patent ID: 7652192

Claim:
A method of producing a transgenic ungulate embryo comprising one or more cells, wherein at least 90% of said one or more cells comprise a human chromosome fragment of greater than 100 kilobase pairs, by transfer of a nuclear donor cell into an enucleated ungulate recipient oocyte, the method comprising: (a) fusing a nuclear donor cell, or a nucleus thereof, with an enucleated ungulate recipient oocyte to form a transgenic ungulate embryo, wherein a human chromosome fragment of greater than 100 kilobase pairs is introduced into said nuclear donor cell or oocyte prior to or following said fusing, whereby said transgenic ungulate embryo comprises said human chromosome fragment; and (b) activating said transgenic ungulate embryo to yield an embryo comprising one or more cells, wherein at least 90% of the one or more cells comprise said human chromosome fragment.