Patent ID: 8318010

Claim:
A chromatography column, having a longitudinal axis, for use in a liquid chromatography process, said column comprising: a chromatography tube; a piston tube having an outer diameter smaller than an inner diameter of said chromatography tube; said piston tube having at least one porous layer partially obstructing flow through the piston tube; at least one chromatography clamp being selectively movable between open and closed positions, and configured to extend substantially 360 degrees about the periphery of said chromatography tube when closed, with no single component of said chromatography clamp extending substantially 360 degrees about said chromatography tube; said chromatography clamp having a clamp ledge extending at an oblique angle relative to the transverse direction, the clamp ledge configured to engage the collar ledge to cam the clamp towards the closed position upon application of greater than atmospheric pressure within the column; said chromatography clamp configured to releasably maintain said chromatography tube and piston tube in fluid communication with one another.