Patent ID: 8314211

Claim:
A method of sealing injured tissue of a patient's body that utilizes a four-part hemostatic composition including a hydrogel carrier and a fibrin monomer, in combination, for cessation of blood loss from the injured tissue without application of a compressive force independently therefrom, the method comprising the steps of: (a) mixing in a mixing device the following components so as to create a foam: (i) a first component (Part A), in a liquid form, of Teleostean (fish) gelatin type A mixed with sucrose, polyvinylpyrrolidone, and Bovine serum albumin in a selected buffer solution at a pH of about 8.3 in the presence of metallic ions; (ii) a second component (Part B) of a selected, relatively high molecular weight acrylic acid of carbomer and divalent ions in a solution at a pH of about 3.4; (iii) a third component (Part C) of a fibrin monomer in a selected acidic solution that polymerizes upon change in pH; and (iv) a fourth component (Part D) having a selected calcium independent transglutaminase enzyme and calcium chloride for stabilizing the fibrin polymer; and (b) delivering the foam through an outlet of the mixing device to a surgical site of a body cavity, organ or tissue so as to place the foam in contact with the site such that the non-dynamic, non-crosslinked fibrin monomer of the foam sticks to wet tissue of the site and forms a matrix in the patient's blood; wherein the mixing of Parts A, B, C, and D renders the non-crosslinked fibrin monomer dynamic by neutralizing the pH of the overall mixture such that the non-crosslinked fibrin is converted to a fibrin sealant, thereby inducing coagulation of the blood and adhesive properties of severed tissue.