Patent ID: 8034225

Claim:
A pulsed field electrophoresis chamber with contour clamped homogeneous electric field (CHEF) electrode array for separating DNA molecules immobilized in agarose plugs loaded into a minigel by means of using a system for energizing their electrodes and alternating the direction of application of the electric field generated by the electrode array, and for circulating the buffer at high flow velocity, which CHEF chamber comprises: a minigel placed in a zone crossed by lines of force of the electric field interacting directly with DNA molecules loaded into said minigel, wherein said zone is the single useful electrophoresis zone (UEZ) of said chamber; and pairs of electrodes of opposite polarities in the electrode array separated a distance ‘d’, which is from 6.2 to about 15 cm, wherein the separation between said pairs of electrodes of opposite polarities in conjunction with the size of said single UEZ determine the area of said chamber, the minigel size, and the total number of samples that can be loaded simultaneously into said minigel; said electrodes being elongate and; a system for attenuating the turbulences of the buffer, said system comprises two rectangular sheets named A and B type sheets, sheets with the same width but different height, being the A type the tallest sheet, sheets alternating disposed from a buffer inlet toward said electrode array with an A type sheet followed by a B type sheet repeating ‘n’ times and wherein ‘n’ is an integer between 1 and 4 and the last sheet before said electrode array is of said A type.