Patent ID: 8119146

Claim:
A process for preparing a multivalent immunogenic composition, comprising the steps of: (a) preparing a HBV component by purifying HBsAg after expression in a S. cerevisiae host carrying a plasmid having a HBsAg coding sequence, wherein the plasmid includes: (1) an upstream promoter from a glyceraldehyde-3-phosphate dehydrogenase gene, for controlling expression of the HBsAg coding sequence; and (2) an ARG3 transcription terminator downstream of the HBsAg coding sequence; (b) preparing at least one immunogenic non-HBV component; and (c) mixing the HBV and non-HBV components to give the multivalent composition, wherein the upstream promoter comprises the nucleotide sequence of SEQ ID NO:1.