Patent ID: 8287854

Claim:
A method of producing a skin equivalent comprising: providing a scaffold, providing mammalian cells derived from stem/progenitor cells isolated from the amniotic membrane of umbilical cord and cultivated in serum free media, wherein said cells derived from stem/progenitor cells are selected from the group consisting of mesenchymal stem/progenitor cells (UCMC) and epithelial stem/progenitor cells (UCEC), placing said UCMC in or onto said scaffold, incubating said scaffold in a first medium adapted for the cultivation of fibroblast, which allows said UCMC to proliferate and further differentiate into fibroblasts, placing said UCEC in or onto said scaffold, and incubating said scaffold in a second medium which allows said UCEC to proliferate and further differentiate into keratinocytes, thereby forming a dermal layer composed of fibroblasts and an epithelial layer composed of keratinocytes.