Patent ID: 8586360

Claim:
A method of producing erythrocytes, comprising expanding a population of human hematopoietic cells in a medium in the absence of feeder cells and in contact with one or more factors, wherein a plurality of human hematopoietic cells within said population of human hematopoietic cells differentiate into erythrocytes during said expanding; and isolating said erythrocytes from said medium, wherein said factors comprise SCF at a concentration of about 10 to about 10,000 ng/mL, IL-3 at a concentration of about 0.01 to about 500 ng/mL, and Epo at a concentration of about 0.1 to about 10 IU/mL, wherein said medium comprises insulin-like growth factor 1 (IGF-1) at a concentration of about 1 to about 1000 ng/mL, wherein said medium does not comprise one or more of Flt-3L, IL-11, thrombopoietin (Tpo), homeobox-B4 (HoxB4), or methylcellulose, and wherein said SCF, IL-3 and Epo are not comprised within an undefined component of said medium.