Patent ID: 6841348

Claim:
A method for determining an adjusted expression measurement for a target gene in a biological sample comprising: measuring the expression of said target gene in said biological sample to obtain an expression measurement of said target gene and simultaneously measuring the expression of at least ten maintenance genes in said first biological sample to obtain an expression measurement for each of said at least ten maintenance genes wherein said at least ten maintenance genes are selected from the group consisting of the genes encoding Profilin, Thymosin beta-4 mRNA, Prothymosin alpha mRNA (ProT-alpha), Actin depolymerizing factor, Adducin gamma subunit, Myosin regulatory light chain, Non-muscle type cofilin, Myeloid cell differentiation protein (MCL1), G Protein Pathway Suppressor 1, Histone class C, Proteasome subunit HsC10-II, Nuclear ribonucleoprotein particle (hnRNP) C protein, ADP-ribosylation factor 1, Integral membrane protein calnexin, Esterase D, ATP-citrate lyase, AQP3 aquaporine 3 (water channel), and Voltage dependent anion channel isoform 1 (VDAC); and adjusting the expression measurement of said target gene using the expression measurement of each of said at least ten maintenance genes to obtain an adjusted expression measurement for said target gene in said biological sample.