Patent ID: 7148024

Claim:
An immunoassay for detecting busulfan in a sample comprising providing a mixture of a sample, containing an antibody selectively reactive with busulfan and not substantially cross-reactive with tetramethylene sulfone, tetrahydrothiophene and tetrahydrothiophene-3-ol-1,1-dioxide and a conjugate of a carrier with a compound of the formula: wherein A is lower alkylene; X′ is a functional linking group; Y is an organic spacing group; X is a terminal functional group capable of binding to a carrier; p and z are independent integers of from 0 to 1; or a compound of the formula wherein A′ is lower alkylene or lower alkenylene; X′, Y, X and z are as above or mixtures thereof, causing the busulfan in the sample and said conjugate to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of busulfan in the sample can be determined.