Patent ID: 7371413

Claim:
A method for the preparation of a kombo butter acid extract from kombo butter, the method comprising: (i) Extraction of the kombo butter with a 70-100% ethanol:water portion to obtain a 70-100% ethanol:water fraction and a first residue; (ii) Separation of the 70-100% ethanol:water fraction and the first residue; (iii) Extraction of the 70-100% ethanol:water fraction with a first non-polar organic solvent portion; (iv) Separation of the 70-100% ethanol:water fraction and the first non-polar organic solvent portion; (v) Evaporation of the 70-100% ethanol:water fraction to obtain a crude kombo butter extract; (vi) Washing of the crude kombo butter extract with an aqueous solution comprising approximately 5-30% of an inorganic base to obtain a solution of kombo butter acid salts and a second residue; (vii) Separation of the solution of kombo butter acid salts and the second residue; (viii) Acidification of the solution of kombo butter acid salts to obtain a formulation of kombo butter acids; (ix) Extraction of the formulation of kombo butter acids with a second non-polar organic solvent portion to obtain an aqueous fraction and an organic fraction; (x) Separation of the aqueous fraction and the organic fraction; and (xi) Evaporation of the organic fraction to obtain the kombo butter acid extract.