Patent ID: 6960432

Claim:
A method for dry detection/quantification of targeted nucleotide chains, comprising the steps of: (1) forming a hybrid (C) of a certain amount of a targeted nucleotide chain (A), and a probe nucleotide chain (B), which has a base sequence complementary to a specific site of the base sequence of said targeted nucleotide chain, on a solid-phase substrate by mutually reacting the two types of nucleotide chains with each other, and in which there exists a fluorescence dye (D), which acts on said hybrid (C), thereby emits fluorescence or increases its fluorescence intensity, and is capable of continuing to emit fluorescence even in a dried state while acting on said hybrid; (2) drying said hybrid (C) and said fluorescence dye (D) on said substrate; and (3) measuring the fluorescence emitted from said fluorescence dye (D), as measuring means, after the drying operation.