Patent ID: 7067310

Claim:
A method for preparing a recombinant adenoviral vector containing an exogenous DNA sequence comprising the steps of: (a) introducing into a prokaryotic cell: (i) a first DNA fragment comprising all or part of an adenoviral genome, including the encapsidation region and the 3′ and 5′ITRs and (ii) a second DNA fragment comprising said exogenous DNA sequence surrounded by flanking sequences A and B which are homologous to (i), and (b) culturing the prokaryotic cell obtained in (a) under suitable culture conditions to allow intermolecular homologous recombination and insertion of the exogenous DNA into the first DNA fragment to occur and recovering the resulting recombinant adenoviral vector; wherein the recombinant adenoviral vector comprises said adenoviral genome including the encapsidation region and the 3′ and 5′ITRs and lacking all or part of the E1, E2, E3, and/or E4 regions and further comprising the exogenous DNA sequence.