Patent ID: 7090804

Claim:
A kit for the detection of an amplification reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising: a) a first nucleic acid primer substantially complementary to at least a first domain of said first target sequence; b) a first label probe substantially complementary to at least a second domain of said first target sequence, said first label probe comprising a plurality of electron transfer moieties (ETM); c) a second nucleic acid primer substantially complementary to at least a first domain of said second target sequence; d) a second label probe substantially complementary to at least a second domain of said second target sequence, said second label probe comprising a plurality of electron transfer moieties (ETM); and, e) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence.