Patent ID: 8450085

Claim:
A method of separately inducing a target protein in a living cell comprising: i. providing an isolated living cell having a mRNA sequence; ii. providing a vector comprising a promoter-operator region of tetA P0 and tetR genes from Tn10; a multiple cloning site downstream of the tetA P0 gene; and mutated nucleic acid sequences encoding the mRNA interferase polypeptide which produces mRNA free from the nucleotide sequence ACA, and the target protein under the controls of the E. coli lac promoter; iii. transforming the isolated living cell by introducing the vector into the isolated living cell; iv. contacting the transformed isolated living cell with tetracycline to induce only the mRNA interferase polypeptide thereby inducing the mRNA interferase polypeptide; and v. contacting the transformed isolated living cell with IPTG to induce the target protein after the induction of the mRNA interferase polypeptide, thereby separately inducing the target protein.