Patent ID: 8192937

Claim:
A method for quantitative determination of a target non-protein coding RNA, comprising the steps of: a) adding a nucleotide sequence to the 3′-end of the target RNA by nucleotide polymerization; b) hybridizing a single-stranded reverse transcription (RT) tagging probe to the target RNA, wherein said probe comprises a sequence complementary to the 3′-end of the target RNA and a sequence for subsequent amplification of the nucleic acid by polymerase chain reaction (PCR) in real-time quantitative PCR (qPCR); c) performing reverse transcription to obtain a strand of cDNA; d) hybridizing a primer comprising a sequence corresponding to the 5′-end of the target RNA to the cDNA of step (c) and generating a double-stranded target sequence; e) performing qPCR including as template said double-stranded target sequence of step (d), wherein said primer comprises a nucleotide analog.