Patent ID: 8685674

Claim:
A method for producing a saccharide chain of Formula (4), the method comprising at least the step of causing a reagent to contact with a GlcUA donor and a saccharide chain of Formula (2): GalNAc-GlcUA-R 2 (2), GlcUA-GalNAc-GlcUA-R 2 (4), where GlcUA is an N-glucuronic acid residue, GalNAc is an N-acetylgalactosamine residue, â€œ-â€ indicates a glycoside linkage, and R 2 is an arbitrary group, and the reagent has an enzyme that has an amino acid sequence including an amino acid sequence (A) or (B) and has catalytic activities (Î±) and (Î²); wherein: (A) is the amino acid sequence from amino acid numbers 47 to 802 in SEQ ID NO: 2; (B) is the amino acid sequence from amino acid numbers 47 to 802 in SEQ ID NO: 2, wherein one to forty amino acids in the amino acid sequence are substituted, deleted, or inserted; (Î±) is the catalytic activity that transfers GalNAc from UDP-GalNAc to chondroitin, where UDP is uridine 5â€² diphosphate, and GalNAc is N-acetylgalactosamine residue; and (Î²) is the catalytic activity that transfers GlcUA from UDP-GlcUA to chondroitin, where UDP is uridine 5â€² diphosphate and GlcUA is glucuronic acid residue.