Patent ID: 8771728

Claim:
A noncovalent nucleic acid/lipid/polycation complex having a net positive charge at pH 6.0-8.0 comprising at least one cationic lipid and a polycation in a ratio of nucleic acid to lipid to polycation which results in the production of said complex, said polycation being selected from the group consisting of polyarginine, polyornithine, polybrene (hexadimethrine bromide), histone, cationic dendrimer, spermine, spermidine, protamine, and synthetic peptides derived from SV40 large T antigen and comprising a nuclear localization sequence, wherein said polycation does not comprise a targeting factor directed to a particular tissue or cell type; and, wherein the complex is formed by adding a nucleic acid to a mixture comprising the at least one cationic lipid and the polycation.