Patent ID: 8273372

Claim:
A process for preparing an extracellular matrix composition which comprises: (a) mixing an aqueous solution of fibrinogen with a coagulating agent and a bulking agent and a foaming agent, wherein the mixing step (a) is achieved by foaming; (b) incubating the mixture obtained in step (a) with a cross-linking agent selected from: carbodiimide coupling agents including N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC); N-hydroxysuccinimide (NHS), azide coupling agents; diisocyanate cross-linking agents including hexamethylene diisocyanate; epoxide cross-linking agents including epi-chlorhydrin, glycidylethers and glycidylamines; and aldehyde cross-linking agents including formaldehyde, glutaraldehyde and glyoxal; and (c) washing the cross-linked composition obtained in step (b) to remove the cross-linking agent.