Patent ID: 7087808

Claim:
A method for producing a transgenic swine whose somatic and germ cells comprise transgenes, comprising the steps of: (a) constructing (i) an expression plasmid that comprises two transgenes wherein DNA sequences of said transgenes encode human clotting factor IX (hFIX) and porcine lactoferrin, respectively, wherein both transgenes are operably linked to a mammary gland specific promoter, or (ii) two expression transgenes, wherein one comprises a DNA sequence of transgene encoding human clotting factor IX (hFIX) operably linked to a mammary gland specific promoter, the other comprises a DNA sequence encoding porcine lactoferrin operably linked to a mammary gland specific promoter and the ratio of said two expression transgenes is 1:1; (b) introducing said expression plasmid or transgenes into a swine embryo; (c) transplanting said embryo comprising said expression transgene or transgenes into a recipient; and (d) allowing said embryo to develop into a transgenic swine, wherein expression of said transgenes result in the production and secretion of hFIX and porcine lactoferrin on the mammary tissue of said swine.