Patent ID: 7381527

Claim:
A method for predicting the susceptibility of an individual to pulmonary tuberculosis, said method comprising: a) amplifying genomic DNA of pulmonary tuberculosis patients and normal control individuals using oligonucleotide primers for PCR amplification of the polymorphic site containing A or G base region containing exon-4 of human SP-A2 gene to obtain an amplified product, b) sequencing the amplified PCR product and identifying the sequence variation computationally by comparing it with the human SP-A2 gene, c) establishing the association of C and G haplotypes as defined by the nucleotide present at position 1649 and 1660, with pulmonary tuberculosis, based on the haplotype frequency distribution in normal individuals and tuberculosis patients, and d) predicting the risk or susceptibility to pulmonary tuberculosis based on the haplotype present at the polymorphic sites in the said individual, wherein a haplotype with C at 1649 position and A at 1660 position is at low risk and a haplotype with G at 1649 position and G at 1660 position is at high risk for the disease.