Patent ID: 7268219

Claim:
A complex comprising an HLA class I molecule or fragment thereof having a peptide binding groove, the HLA class I molecule or fragment thereof comprising a T cell recognition portion, and an attachment means for selectively attaching said HLA class I molecule or fragment thereof to a target cell, wherein the HLA class I molecule or fragment thereof is bound or is attached to a recognition peptide in the peptide binding groove of the HLA class I molecule or fragment thereof, wherein the recognition peptide is arranged to be presented by said HLA class I molecule or fragment thereof for T cell recognition, wherein the attachment means comprises: a) a linking polypeptide with specific affinity for a molecule on the surface of the target cell; and b) a coupling system for coupling the linking polypeptide to the HLA class I molecule or fragment thereof, wherein the coupling system comprises: (i) a first small molecule joined to the linking polypeptide; and (ii) a second small molecule joined to the HLA class I molecule, wherein the first and second small molecules are each selected from biotin and avidin/streptavidin or calmodulin and calmodulin binding peptides; wherein interaction of the small molecules forms a stable bridge between the linking polypeptide and the HLA class I molecule.