Patent ID: 8372612

Claim:
A recombinant bacterial cell producing butanol or 2-butanone said bacterial cell comprising at least one genetic modification which reduces accumulation of (p)ppGpp, wherein the at least one genetic modification is a disruption in an endogenous gene encoding SpoT or RelA or in an operon comprising an open reading frame encoding SpoT or RelA; a) the SpoT protein having: 1) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the RelA/Spot domain; and 2) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the TGS domain; and 3) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the HD domain; and b) the RelA protein having: a) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the RelA/SpoT domain; and b) an E-value parameter of 0.01 or less when queried using the Pfam Profile HMM for the TGS domain.