Patent ID: 8691576

Claim:
A method for regenerating fertile plants of the genera Jatropha through somatic embryogenesis and a two step induction process with callus formation comprising: (a) culturing an explant of the genera Jatropha on a solid hormone-containing medium for a period sufficient to initiate calli growth, wherein the hormone is 2,4-dichlorophenoxyacetic acid (2,4-D) at a concentration of from about 0.5 mg/l to about 10 mg/1; (b) culturing the explant or calli on a hormone-free medium for a period sufficient to develop somatic embryos or embryogenic calli; (c) culturing the somatic embryos or embryogenic calli on a solid medium or in a liquid medium to propagate the somatic embryos or embryogenic calli, wherein the liquid medium contains 2,4-D in an amount from about 0.1 mg/l to about 0.5 mg/1; (d) culturing the somatic embryos on a solid maturation medium for a period of time sufficient to mature the somatic embryos, wherein the maturation medium contains from about 10 g/l to about 20 g/l sucrose or from about 20 g/l to about 60 g/1 maltose; and (e) culturing the matured somatic embryos on a germination medium for a period of time sufficient to germinate the somatic embryos to produce plantlets, wherein the germination medium contains giberellic acid (GA 3 ) at a concentration of from about 0.5 mg/l to about 5 mg/l.