Patent ID: RE44522

Claim:
A method for measuring a concentration of a test target, the method comprising: constructing a reaction system by supplying whole blood containing the test target to a reagent layer containing an oxidation-reduction enzyme and an electron mediator; measuring the concentration of the test target by utilizing amperometry ; wherein the test target is glucose, the oxidation-reduction enzyme is CyGDH, and a Ru compound ruthenium(III) complex is used as the electron mediator, the method further comprising: applying constant voltage to the reagent layer; dissolving the electron mediator directly in the whole blood containing the test target to form a solution; detecting the solution by applying the voltage to the reagent layer for measurement; and computing the concentration of the test target on the basis of a response current value from the reagent layer; wherein the constant voltage is no greater than 300 mV and selected from a range from a standard oxidation-reduction potential (versus a standard hydrogen electrode) between an oxidant and a reductant of the Ru compound electron mediator through a standard oxidation-reduction potential (versus a standard hydrogen electrode) between ferrocyanide ions and ferricyanide ions , wherein the concentration of the test target is measured at any point between 3 and 15 seconds after the whole blood containing the test target is supplied to the reagent layer, and wherein the system is configured to be without any interferent mitigating elements .