Patent ID: 7888500

Claim:
A method for preparing sugar C-glycoside derivatives comprising: a) reacting an aldose reducing sugar with a β-diketone under conditions and for a period of time effective to form a C-glycoside ketone, which said aldose reducing sugar comprises an aldehyde containing hexose aldose reducing sugar or an aldehyde containing pentose aldose reducing sugar, wherein said hexose is optionally substituted at one or more of the C2, C3, C4, C5, and C6 positions thereof and said pentose is optionally substituted at one or more of the C2, C3, C4, and C5 positions thereof, and b) reacting said C-glycoside ketone with a ketone reactive compound, which said ketone reactive compound is optionally linked to a detectable label or solid surface, under conditions and for a period of time effective to form a C-glycoside derivative wherein said ketone reactive compound is conjugated at the carbon of the ketone of said C-glycoside ketone, and the ring structure of said sugar remains intact, wherein said ketone reactive compound is selected from the group consisting of hydrazines or hydrazides, semicarbazides, and thiols, and said C-glycoside derivative is selected from the group consisting of C-glycoside ketohydrazones, C-glycoside semicarbazones, and C-glycoside thiolketones, respectively.