Patent ID: 8865463

Claim:
A method of preparing a self-propagating pancreatic beta cell specific to a human patient, the method comprising: (a) obtaining a human cancer cell; (b) treating the human cancer cell with a DNA interchelating agent to obtain a DNA interchelating agent treated human cancer cell; (c) treating the DNA interchelating agent treated human cancer cell with an agglutinin to obtain an agglutinin-treated human cancer cell; (d) fusing the agglutinin-treated human cancer cell from step (c) with a human pancreatic beta cell to obtain a self-propagating human pancreatic beta cytoplasmic donor cell; (e) treating the self-propagating human pancreatic beta cytoplasmic donor cell from step (d) with a DNA interchelating agent to obtain a DNA interchelating agent treated human pancreatic beta cytoplasmic donor cell; (f) treating the DNA interchelating agent treated human pancreatic beta cytoplasmic donor cell from step (e) with an agglutinin to obtain an agglutinin-treated human pancreatic beta cytoplasmic donor cell; (g) fusing the agglutinin-treated human pancreatic beta cytoplasmic donor cell from step (f) with a histone deacetylase inhibitor treated cell from a human patient whereby a self-propagating pancreatic beta cell specific to the human patient is obtained.