Patent ID: 7794983

Claim:
A method for genetic detection, comprising: carrying out a polymerase chain reaction (PCR) on a DNA sample with a primer set to produce amplified DNA products, the primer set comprising a first primer for both a filled site containing an interspersed element and an empty site which does not contain the interspersed element, a second primer for the filled site, and a third primer for the empty site, the first primer complementary to a first flanking genomic sequence in the filled site and the empty site, the second primer complementary to a sequence comprised of a second flanking genomic sequence, a direct repeat sequence next to the second flanking genomic sequence and a part of the interspersed element next to the direct repeat sequence in the filled site, the third primer complementary to a sequence comprised of the direct repeat sequence and third flanking genomic sequences on both sides of the direct repeat sequence in the empty site; and analyzing the amplified DNA products to detect presence or absence of the interspersed element insertion at the site.