Patent ID: 7521531

Claim:
A method for preparing and purifying a conjugate of a radioiodinated aminopolycarboxylate-appended peptide and a targeting agent, comprising one-pot method for preparation and purification of said conjugate without a column purification step, comprising the steps of: (A) contacting a solution comprising; (i) unbound radioiodine; (ii) a radioiodinated aminopolycarboxylate-appended peptide (APC-peptide) that is not conjugated to a targeting agent; and (iii) a radioiodinated aminopolycarboxylate-appended peptide that is conjugated to the targeting agent, wherein the aminopolycarboxylate-appended peptide is selected from the group consisting of: X-Gly-D-Tyr-D-Lys((1-(p-CSNH)benzyl)DTPA)-OH; X-D-Ala-D-Tyr-D-Tyr-D-Lys(DTPA); [X-D-Ala-D-Tyr-D-Tyr-D-Lys(½ DTPA)] 2 ; X-Lys(X)-Lys((1-(p-CSNH)benzyl)DTPA)-D-Tyr-D-Tyr-D-Lys((1-(p-NH)benzyl)DTPA)-OH; X-Lys(X)-Lys((1-(p-CSNH)benzyl)DTPA)-D-Tyr-D-Lys((1-(p-CSNH)benzyl)DTPA)-OH; X-Asp-D-Tyr-D-Lys((1-(p-CSNH)benzyl)DTPA)-OH; X-Lys(MCC)-Asp-D-Tyr-D-Lys((1-(p-CSNH)benzyl)DTPA)-OH; X-Asp-D-Tyr-D-Lys-((1-(p-CSNH)benzyl)DTPA)-OH; and X-Lys(X)-Asp-D-Tyr-D-Lys((1-(p-CSNH)benzyl)DTPA)-OH wherein X is a cross-linker comprising a linking moiety that forms a covalent link with the targeting agent; with an anion exchange resin; and (B) filtering the solution through a filter capable of trapping anion-exchange resin particles; wherein the anion exchange resin binds to both unbound radioiodine and radioiodinated APC-peptide and wherein purified radioiodinated APC-peptide conjugated to targeting agent is obtained in the filtrate.