Patent ID: 8131480

Claim:
A computer-implemented method for diversity analysis of a database comprising related amino acid sequences characterized by at least one shared sequence motif and synthesis of a polypeptide library based on said analysis, comprising the steps of: (a) aligning said related amino acid sequences; (b) generating a first dataset representing a subset of the related amino acid sequences by applying to said amino acid sequences a predetermined combination of two or more filters based on parameters of the amino acid sequences; (c) analyzing said first dataset for positional amino acid usage frequency within said shared sequence motif; (d) generating a second dataset that models positional amino acid diversity within the shared sequence motif for amino acid sequences characterized by said predetermined combination of filters, by applying a minimum threshold amino acid usage frequency to the positional amino acid usage frequency obtained in step (c) at one or more amino acid positions within said shared sequence motif (e) synthesizing a physical library of related amino acid sequences that is designed with the aid of the second dataset, characterized in that: synthesis of the physical library comprises synthesizing a collection of degenerate oligonucleotide sequences that physically represent the combinatorial positional amino acid diversity of the second dataset and cloning the oligonucleotide sequences into a suitable template.