Patent ID: 7754453

Claim:
A method for detecting the production of a primer extension product in a primer extension reaction mixture, said method comprising: a) producing a primer extension mixture comprising a nucleic acid polymerase, a FET labeled probe, said FET labeled probe comprising a nucleic acid intercalator bonded to a FET labeled oligonucleotide, wherein said nucleic acid intercalator is covalently bonded to the 3′ end of said FET labeled oligonucleotide; b) subjecting said primer extension mixture to primer extension reaction conditions; c) detecting a change in a fluorescent signal from said FET labeled oligonucleotide probe to obtain an assay result; and d) employing said assay result to determine whether a primer extension product is present in said mixture, wherein said mixture includes a nucleic acid polymerase having 3′→5′ exonuclease activity.