Patent ID: 8648187

Claim:
A method for the separation of double-stranded nucleic acid from single-stranded nucleic acids, which method comprising: a) mixing a sample containing said double-stranded nucleic acid and said single-stranded nucleic acid with a solution containing (1) a chaotropic salt and (2) one or more non-ionic detergent having a combined concentration of from 0.5% to 10% to generate a mixture; b) applying said mixture to a first mineral support for the double-stranded nucleic acid to bind; c) collecting a flow-through which contains unbound single-stranded nucleic acid; d) diluting the one or more non-ionic detergent of said flow-through to 0.1%; and e) applying the adjusted flow-through from step (d) to a second mineral support for the single-stranded nucleic acid to bind.