Patent ID: 7867781

Claim:
A method for detecting an analyte A in a sample, comprising: (i) incubating an incubation mixture comprising a sample with an analyte A-specific binding partner R1, which is associated with a solid phase, an analyte A-specific binding partner R2, which is associated with a label L1, and an analyte A-specific binding partner R3, which is associated with a label L2, wherein binding partners R2 and R3 are selected such that saturation of analyte A-binding sites of the binding partner R2 requires a) a higher analyte A concentration, b) a longer incubation, or c) a higher analyte A concentration and a longer incubation, than does saturation of analyte A-binding sites of the binding partner R3; and (ii) determining an L1-dependent measurement signal at time T1 and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal at time T2, wherein time T1 is earlier than time T2, time T2 is after addition of label L2, and time T1 occurs before 30% of time from addition of label L2 to time T2 has elapsed; or determining an L1-dependent measurement signal using a first measurement method and an L2-dependent measurement signal or an L1 plus L2-dependent measurement signal using a second measurement method, wherein the first and second measurement methods are different.