Patent ID: 7981665

Claim:
A method for making a support for performing an assay, comprising: pre-blocking a binding polymer to provide a pre-blocked binding polymer of formula (I); attaching the pre-blocked binding polymer of the formula (I) directly or indirectly to a substrate; the polymer having a plurality of maleic anhydride reactive groups (m) for attaching a biomolecule and a plurality of inactive groups (n), where X is a divalent NH, O, or S; R is H, or a substituted or an unsubstituted, linear or branched, alkyl group, an oligo(ethylene oxide), an oligo(ethylene glycol), or a dialkyl amine; R′ is a residue of a first unsaturated monomer that has been copolymerized with maleic anhydride; the relative ratio (m:n) of the maleic anhydride reactive groups to the inactive groups is from 0.5 to 10 in the attached pre-blocked binding polymer, and the attached pre-blocked binding polymer does not contain a photoreactive group.