Patent ID: 8088392

Claim:
An immunogenic composition comprising macro-molecular structures containing multi-units of fusion protein comprising a capsid protein, a chaperone protein and a desired antigen or antigens or epitope(s) joined together by peptide bond(s); wherein the said capsid protein has the intrinsic ability of self-assembling into virus-like particles; and the said macro-molecular structures are obtained by: (a) recombinantly producing the said fusion protein comprising a capsid protein, a chaperone protein and a desired antigen(s) joined together by peptide bonds by an expression system; (b) separating and purifying of said fusion protein in the presence of chaotropic agent, thereby obtaining purified fusion protein in denatured form; (c) refolding and reassembling of the denatured fusion protein into macro-molecular structures containing multi-units of fusion protein by a process involving removing out chaotropic agents presented in the denatured fusion protein sample; the said macro-molecular structures are morphologically different from a virus or virus-like particles; wherein the said macro-molecular structures induce immune responses to said desired antigen(s) in a mammal to whom the macro-molecular structures are administered.