Patent ID: 8288530

Claim:
A method of preparing a macromolecular conjugated ligand comprising: (a) providing a compound of formula (I) wherein -A CH 2 COOPg) n is a group of the formula (III) wherein each of R 1 -R 8 is the same or different and is hydrogen, a C 1 -C 12 alkyl group, or a point of attachment of wherein R 1 and R 2 and/or R 3 and R 4 together form a C 5 -C 7 cycloalkyl group, R is Pg is a carboxyl-protecting group, and m is 0 to 3, (b) reacting the compound of formula (I) with a macromolecular compound in an organic solvent medium which is substantially free of water to obtain a carboxyl-protected macromolecular conjugated ligand, and (c) removing the carboxyl-protecting group to obtain a macromolecular conjugated ligand.