Patent ID: 8722363

Claim:
A method for producing a host cell comprising an isoprenoid compound, comprising: a) providing a host cell that comprises heterologous nucleic acid encoding an isoprenoid synthase, and heterologous nucleic acid encoding one or more protein(s) comprising a cytochrome P450 polypeptide, wherein: the isoprenoid synthase catalyzes production of an isoprenoid compound that is a diterpene or sesquiterpene; and a cytochrome P450 polypeptide(s) catalyzes hydroxylation, oxidation, demethylation or methylation of a diterpene or sesquiterpene; b) culturing the host cell under conditions suitable for expressing the isoprenoid synthase and the cytochrome P450 polypeptide(s) and under conditions for producing a diterpene or a sesquiterpene isoprenoid compound not normally produced by the host cell to produce a diterpene or sesquiterpene isoprenoid compound not normally produced by the host cell, wherein the synthase and the cytochrome P450 polypeptide(s) together catalyze the formation of a diterpene or sesquiterpene isoprenoid compound in the host cell, wherein the diterpene or sesquiterpene isoprenoid compound is not normally produced by the cell.