Patent ID: 7060467

Claim:
A method for producing a recombinant protein containing repeating units comprising: (a) providing a first pool of polynucleotides comprising codons, said pool of polynucleotides comprising at least two tandem repeats of sequences encoding a portion of said recombinant protein, wherein said tandem repeats contain degenerate nucleotide sequences encoding for a portion of said recombinant protein in accordance with the degeneracy of the genetic code; (b) providing a second pool of polynucleotides, at least some of which are complementary to the polynucleotides in said first pool of polynucleotides; (c) combining said first pool of polynucleotides and said second pool of polynucleotides under conditions whereby the polynucleotides will anneal; (d) extending the 3′ ends of said annealed polynucleotides under conditions wherein said annealed polynucleotides act as primers for their complements; (e) denaturing the extended polynucleotides; (f) repeating steps (c)–(e) at least once, whereby the products of step (e) provide the polynucleotides for annealing in step (c) of the next cycle; (g) if necessary, adding one or more linker oligonucleotides to the end of the products of (f), said linker oligonucleotides containing at least one restriction enzyme cleavage site; (h) inserting the products of (f) or (g) into a suitable vector; (i) introducing said vector into a suitable host cell; and (j) maintaining said host cell under conditions allowing for expression of said recombinant protein.