Patent ID: 8581210

Claim:
A fluorescence intensity compensation method comprising: emitting light onto a microparticle multiply labeled with a plurality of fluorochromes having fluorescence wavelength ranges that overlap each other in order to excite the fluorochromes and receiving fluorescence generated by the excited fluorochromes using photodetectors having different reception wavelength ranges; and computing fluorescence intensities of the fluorochromes by compensating detection values of the photodetectors under a predetermined constraint condition imposed on the computed fluorescence intensities, wherein the constraint condition is that each of the computed fluorescence intensity of each of the fluorochromes and an autofluorescence intensity is higher than or equal to a predetermined minimum value, wherein the constraint condition further includes a condition that each of the computed fluorescence intensity of each of the fluorochromes and the autofluorescence intensity is lower than or equal to a predetermined maximum value, and wherein in computing fluorescence intensities, an approximation of a measured spectrum using a linear sum of a simple staining spectrum and an autofluorescence spectrum is performed using a least-squares method.