Patent ID: 8603734

Claim:
A method of detecting prostate cancer in a subject, comprising: a. obtaining a serum sample from the subject; b. adding to the sample up to eight isotopically labeled peptides identical to native biomarker peptides derived from protease digestion of the biomarkers prostate specific antigen (KLK3), prostatic acid phosphatase (ACPP), metalloproteinase inhibitor 1 (TIMP1), membrane metalloendopeptidase (MME), and gamma-glutamyltranspeptidase 1 (GGT1), wherein the up to eight isotopically labeled peptides include SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8; c. detecting the presence of the isotopically labeled peptides and unlabeled, native biomarker peptides using mass spectrometry, wherein the presence of the native biomarker peptides in the sample is indicative of prostate cancer.