Patent ID: 8298806

Claim:
A method of preparing an immunogenic composition comprising: (a) providing a culture comprising non-invasive Helicobacter pylori cells transformable with a nucleic acid sequence, that can chronically colonize the mucosa of a mammal, and that are not a DapE mutant strain; (b) introducing into said culture at least one non- Helicobacter nucleic acid sequence encoding a non- Helicobacter immunogen of interest linked to a secretory signal peptide and a promoter capable of controlling expression of the non- Helicobacter sequence in the Helicobacter pylori cells under suitable conditions to produce transformed Helicobacter pylori cells; (c) selecting one or more of the transformed Helicobacter pylori cells of step (b) that express said non- Helicobacter immunogen to provide an immunogenic composition; and (d) formulating the immunogenic composition comprising the one or more transformed Helicobacter pylori cells that express said non- Helicobacter immunogen for administration to a mammal.