Patent ID: 7354715

Claim:
A method of detecting mutant DNA wherein said method comprises the steps of: a) providing at least one DNA sample derived from at least one tissue sample; b) digesting said at least one DNA sample with at least one restriction enzyme to obtain a plurality of restriction fragments; c) providing more than two upstream RF primers that bind upstream of a targeted restriction site on said DNA sample, and more than two downstream RF primers that bind downstream of said targeted restriction site on said DNA sample, said primers binding in orientations that permit generation of an amplicon between any said upstream RF primer and any said downstream RF primer only when a mutation is present in said DNA sample that removes a restriction site in said DNA sample, and wherein said amplicon is not produced in the absence of a mutation; d) subjecting said restriction fragments and said RF primers to PCR amplification conditions to obtain a PCR sample; and e) assaying said PCR sample for the presence of said amplicon that indicates the presence of said mutation that removed the restriction site from said DNA sample.