Patent ID: 7741295

Claim:
A method of determining whether a test compound is an inhibitor of a Sir2 enzyme, the method comprising combining the test compound with the Sir2 enzyme, NAD + and an acetylated peptide substrate of Sir2 in a reaction mixture under conditions and for a time sufficient to deacetylate the peptide in the absence of the test compound; quantifying 2′/3′-O-acetyl-ADP-ribose produced from the acetylated peptide; then comparing the quantity of 2′/3′-O-acetyl-ADP-ribose produced with a quantity of 2′/3′-O-acetyl-ADP-ribose produced under the same conditions but without the test compound; wherein the presence of less 2′-O-acetyl-ADP-ribose and/or 3′-O-acetyl-ADP-ribose than without the test compound indicates that the test compound is an inhibitor of the Sir2 enzyme.