Patent ID: 7052831

Claim:
A kit for performing a flow-through analytical test for detecting the presence of and distinguishing between Influenza A viral antigens and Influenza B viral antigens in a biological sample suspected of containing one or both of Influenza A viral antigens and Influenza B viral antigens, the kit comprising: (a) an analytical test device having a plurality of wells, wherein the wells do not communicate with each other; and wherein each well comprises: a filter stack, the filter stack comprising: (i) a porous membrane having an upper surface and a lower surface; and (ii) an absorbent material, wherein said lower surface of said porous membrane and said absorbent material are in physical contact and in fluid communication, and wherein upon contact of a biological fluid sample suspected of containing one or both of the Influenza A viral antigens and the Influenza B viral antigens with said porous membrane, said fluid is able to flow through said membrane into said absorbent material, such that at least a portion of said viral antigens present in said biological fluid sample bind to said porous membrane; (b) a first detection reagent capable of recognizing Influenza A viral antigens; (c) a second detection reagent capable of recognizing Influenza B viral antigens; and (d) a reagent capable of generating a signal upon detection of one or both of Influenza A viral antigens and Influenza B viral antigens.