Patent ID: 8309347

Claim:
A cell expansion system comprising: a) a first fluid circulation path comprising i) a first fluid flow path having at least opposing ends, a first opposing end of said first fluid flow path fluidly associated with a first inlet port of a hollow fiber membrane containing cell growth chamber and a second opposing end of said first fluid flow path fluidly associated with a first outlet port of said cell growth chamber, wherein said first fluid flow path is fluidly associated with an intracapillary portion of said hollow fiber membrane, and ii) a first fluid controller operably associated with said first fluid flow path; b) a second fluid circulation path comprising i) a second fluid flow path having at least opposing ends, a first opposing end of said second fluid flow path fluidly associated with a second inlet port of said cell growth chamber and a second opposing end of the second fluid flow path fluidly associated with a second outlet port of said cell growth chamber, wherein the second fluid flow path is fluidly associated with an extracapillary portion of said hollow fiber membrane; and ii) a second fluid controller operably associated with said second fluid flow path; c) a first fluid inlet path fluidly associated with said first fluid circulation path to allow fluid into said first fluid circulation path; d) a first fluid outlet path fluidly associated with at least one of said first or second fluid circulation paths; e) at least one fluid connector path having at least opposing ends, a first opposing end of said fluid connector path fluidly associated with said first fluid circulation path and a second opposing end of said fluid connector path fluidly associated with said second fluid circulation path, wherein the at least one fluid connector path comprises a third fluid controller; and f) a second fluid connector flow path having opposing ends, one end of said second fluid connector flow path fluidly associated with said first fluid flow path and a second end of said second fluid connector flow path fluidly associated with said second fluid flow path, wherein the second fluid connector flow path comprises a fourth fluid controller.