Patent ID: 7422670

Claim:
A method for performing gel electrophoresis, comprising: a) obtaining a first running buffer or a second running buffer, wherein the obtaining is based on a molecular weight of a protein, and wherein the molecular weight of the protein used to obtain the first running buffer is larger than the molecular weight of the protein used to obtain the second running buffer; and b) performing electrophoresis to separate the protein using an electrophoresis gel and the obtained running buffer; wherein the electrophoresis gel comprises a separating gel between 8 and 25 (wt/vol)% acrylamide and a gel buffer having a pH between 6 and 8, wherein said gel buffer comprises bis(2-hydroxyethyl)iminotris(hydroxymethyl)methane; and wherein the first running buffer comprises 3-(N-morpholino)propanesulfonic acid (MOPS); and wherein the second running buffer comprises 2-(N-morpholino)ethanesulfonic acid (MES).