Patent ID: 7105300

Claim:
A method for sequencing a nucleic acid, the method comprising: (i) providing a nucleic acid template and a primer; (ii) incubating the nucleic acid template and the primer with a polymerase and one or more nucleotide analogs having formula (III), thereby adding at least one of the one or more nucleotide analogs to the primer, resulting In an extended primer, wherein R 1 comprises a nucleoside, a nucleotide, a nucleatide analog, or a nucleoside analog, R 2 comprises a chain terminating blocking group, which blocking group comprises a detectable label, and R 3 comprises a hydrogen or a negative charge, and wherein R 2 comprises a compound having formula (V) or formula (VI); wherein R 4 comprises one or more of a linker moiety and the detectable label: (iii) detecting the detectable label, thereby detecting the at least one of the one or more nucleotide analogs and removing the blocking group from the extended primer; or, removing the blocking group from the extended primer and detecting the detectable label, thereby detecting the at least one of the one or more nucleotide analogs; (iv) removing a 3′-phosphate from the extended primer, thereby producing an extendable primer; and, (v) repeating steps (ii) through (v), thereby sequencing the nucleic acid template.