Patent ID: 7923245

Claim:
A medium for culturing embryonic stem (ES) cells comprising: (a) insulin, transferrin, 2-mercaptoethanol, 2-ethanolamine, sodium selenite, oleic acid which has formed a complex with fatty acid-free bovine serum albumin, and LIF (leukemia inhibitory factor; and (b) the basal medium comprising the composition shown by the following Table III. TABLE III Components Concentration (mg/L) L-alanine 1.78~2.67 L-arginine 40~60 L-arginine HCl 75.8~113.7 L-asparagine H 2 O 13.002~19.503 L-Asparatic acid 6.66~9.99 L-cysteine HCl•H 2 O 7.024~10.536 L-cystine 2HCl 38.058~57.087 L-glutamic acid 6.94~10.41 L-glutamine 439.72~659.58 Glycine 15.5~23.25 L-histidine 3~30 L-hydroxyproline 4~6 L-isoleucine 52.748~79.122 L-leucine 54.58~81.87 L-lysine HCl 73.74~110.61 L-methionine 15.896~23.844 L-phenylalanine 30.392~45.588 L-proline 10.9~16.35 L-serine 24.9~37.35 L-threonine 44.42~66.63 L-tryptophan 7.808~11.712 L-tyrosine 33.888~50.832 L-valine 43.86~65.79 Glutathione 0.2~0.3 Para-aminobenzoic acid 0.2~0.3 Biotin 0.04148~0.06222 Calcium pantothenate 1.746~2.619 Choline chloride 4.992~7.488 Folic acid 2.06~3.09 Inositol 13.48~20.22 Niacinamide 1.8074~2.7111 Pyridoxal HCl 1.6~2.4 Pyridoxine HCl 0.2124~0.3186 Riboflavin 0.2076~0.3114 Thiamine HCl 1.868~2.802 Vitamin B 12 0.273~0.4095 Hypoxanthine 0.816~1.224 Linoleic acid 0.0168~0.0252 Lipoic acid (thioctic acid) 0.042~0.063 Putrecine dihydrochloride 0.0322~0.0483 Thymidine 0.146~0.219 Sodium chloride 5279.8~7919.7 Potassium chloride 284.72~427.08 Calcium chloride 86.644~129.966 (anhydrous) Calcium nitrate 4H 2 O 20~30 Magnesium chloride 11.444~17.166 (anhydrous) Magnesium sulfate 48.844~73.266 (anhydrous) Sodium dihydrogen 43.48~65.22 phosphate (anhydrous) Disodium monohydrogen 188.408~282.612 phosphate (anhydrous) Glucose (anhydrous) 1860.4~2790.6 Ferric nitrate 9H 2 O 0.04~0.06 Copper sulfate 5H 2 O 0.0005~0.00075 Ferrous sulfate 7H 2 O 0.1668~0.2502 Zinc sulfate 7H 2 O 0.1728~0.2592 Phenol red 5.248~7.872.