Patent ID: 8722967

Claim:
A method for increasing seed size or weight in a plant, said method comprising introducing into the plant an isolated nucleic acid molecule encoding a plant cytokinin oxidase wherein the isolated nucleic acid molecule is expressed in a seedling or in the endosperm, embryo or aleurone of a plant seed, wherein the isolated nucleic acid molecule encoding the cytokinin oxidase is selected from the group consisting of: (a) an isolated nucleic acid molecule comprising the DNA sequence as set forth in SEQ ID NO:1 or 25, or the complement thereof, (b) an isolated nucleic acid molecule comprising the RNA sequence encoding the amino acid sequence of SEQ ID NO:2, or the complement thereof, (c) an isolated nucleic acid molecule encoding the protein comprising the amino acid sequence as set forth in SEQ ID NO:2, or the complement thereof, (d) an isolated nucleic acid molecule as defined in any of (a) to (c) characterized in that said nucleic acid molecule is DNA, genomic DNA, cDNA, synthetic DNA or RNA wherein T is replaced by U, and (e) an isolated nucleic acid molecule specifically hybridising under high stringency conditions to the nucleic acid molecule of (c) wherein the high stringency conditions are hybridization at 4×SSC at 65° C., followed by washing in 0.1×SSC at 65° C., wherein the isolated nucleic acid molecule increases embryo size or weight when expressed in the embryo.