Patent ID: 7112405

Claim:
A method of oligonucleotide-mediated nucleic acid sequence alteration comprising: providing an altered cell or cell-free extract comprising a target nucleic acid sequence, wherein the altered cell or cell-free extract has been altered such that it exhibits increased expression or protein activity relative to unaltered cell or cell-free extract of at least one protein selected from the group consisting of RAD10, RAD51, RAD52, RAD54, RAD55, MRE11, PMS1, XRS2, and combinations thereof, and further wherein said altered cell or cell-free extract exhibits decreased expression or protein activity relative to unaltered cell or cell-free extract of at least one other protein selected from the group consisting of RAD10, RAD51, RAD52, RAD54, RAD55, MRE11, PMS1, XRS2, and combinations thereof; providing an oligonucleotide adapted for altering at least one nucleotide in said target nucleic acid sequence; and introducing said oligonucleotide to the cell or cell-free extract under suitable conditions to allow recombination with the target nucleic acid sequence.