Patent ID: 8852891

Claim:
An in vitro method for preparing a CMP-N-acetylneuraminic acid from N-acetyl-D-glucosamine, the method comprising: reacting a reaction mixture containing (i) cytidine 5′-monophosphate (CMP), (ii) nucleotide triphosphate (NTP) or ATP, (iii) acetyl phosphate (Pi), (iv) N-acetyl-D-glucosamine and (v) pyruvic acid, in the presence of (a) N-acetylglucosamine-2-epimerase (GlcNAc 2-epimerase), (b) N-acetylneuraminic acid aldolase (NeuNAc aldolase), (c) cytidine 5′-monophosphate kinase (CMP kinase), (d) acetate kinase and (e) CMP-N-acetylneuraminic acid synthetase (CMP-NeuNAc synthetase); and recovering said CMP-N-acetylneuraminic acid.