Patent ID: 7602494

Claim:
A method of imaging fluorescence, in which an inverted fluorescence imaging apparatus provided with an immersion objective is used to image an observation phosphor for a constant term, comprising: an imaging position locating step of locating a vessel incorporating the observation phosphor at an imaging position of the fluorescence imaging apparatus; a liquid pouring step of pouring a preset amount of liquid between a top portion of the immersion objective in the fluorescence imaging apparatus and the vessel incorporating the observation phosphor to bring about a charged state; an imaging step of imaging the observation phosphor in the vessel in a state where a space between the top portion of the immersion objective and the vessel is charged with the liquid; and a repeated control step of repeating a series of operations including the liquid pouring step of a single operation and the imaging step of a preset number of times of operations performed after the liquid pouring step of the single operation, at preset time intervals.