Patent ID: 8741582

Claim:
A method of detecting bladder cancer in a human, comprising the steps of: (a) obtaining a urine sample collected from a human, said human is suspected of suffering from bladder cancer; (b) concentrating said urine sample; (c) immobilizing a first anti-DEK antibody onto a solid surface, said first anti-DEK antibody recognizes amino acids 1-48 or 83-375 of DEK protein consisting of SEQ ID NO: 3; (d) adding said concentrated urine sample onto said solid surface after step (c) so as to allow said immobilized first anti-DEK antibody to capture DEK protein onto said solid surface; (e) removing unbound protein; (f) adding a second anti-DEK antibody, said second anti-DEK antibody recognizes amino acids 1-48 or 83-375 of DEK protein, so as to allow said second anti-DEK antibody to bind to said captured DEK protein to form a complex wherein said first anti-DEK antibody and said second anti-DEK antibody recognize a different region of DEK protein; and (g) detecting said complex, wherein the presence of said complex is indicative of occurrence of bladder cancer in said human.