Patent ID: 8853362

Claim:
A single-chain antibody comprising a variable light chain framework VL and a variable heavy chain framework VH of a human and naturally occurring antibody which is highly stable and soluble under reducing conditions having the general structure: NH 2 -VL-linker-VH-COOH; or NH 2 -VH-linker-VL-COOH wherein the single-chain antibody has the VH framework and the VL framework of AJ, BJ, CJ, DJ, EJ, FJ, or GJ; wherein A is the amino acid sequence (Seq. Id. No. 1) EIVMTQSPSTLSASVGDRVIITCRASQSISSWLAWYQQKPGKAPKLLIYK ASSLESGVPSRFSGSGSGAEFTLTISSLQPDDFATYYCQQYKSYWTFGQG TKLTVLG; B is the amino acid sequence (Seq. Id. No. 2) EIVLTQSPSSLSASVGDRVTLTCRASQGIRNELAWYQQRPGKAPKRLIYA GSILQSGVPSRFSGSGSGTEFTLTISSLQPEDVAVYYCQQYYSLPYMFGQ GTKVDIKR; C is the amino acid sequence (Seq. Id. No. 3) EIVMTQSPATLSVSPGESAALSCRASQGVSTNVAWYQQKPGQAPRLLIYG ATTRASGVPARFSGSGSGTEFTLTINSLQSEDFAAYYCQQYKHWPPWTFG QGTKVEIKR; D is the amino acid sequence (Seq. Id. No. 4) QSVLTQPPSVSAAPGQKVTISCSGSTSNIGDNYVSWYQQLPGTAPQLLIY DNTKRPSGIPDRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSSLSGVV FGGGTKILTVLG; E is the amino acid sequence (Seq. Id. No. 5) EIVLTQSPATLSLSPGERATLSCRASQTLTHYLAWYQQKPGQAPRLLTYD TSKRATGVPARFSGSGSGTDFTLTISSLEPEDSALYYCQQRNSWPHTFGG GTKLEIKR; F is the amino acid sequence (Seq. Id. No. 6) SYVLTQPPSVSVAPGQTATVTCGGNNIGSKSVHWYQQKPGQAPVLVVYDD SDRPSGIPERFSGSNSGNTATLTIRRVEAGDEADYYCQVWDSSSDHNVFG SGTKVEIKR; G is the amino acid sequence (Seq. Id. No. 7) LPVLTQPPSVSVAPGQTARISCGGNNIETISVHWYQQKPGQAPVLVVSDD SVRPSGIPERFSGSNSGNTATLTISRVEAGDEADYYCQVWDSSSDYVVFG GGTKLTVLG; H is the amino acid sequence (Seq. Id. No. 8) QVQLVQSGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSA ISGSGGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAAHV LRFLEWLPDAFDIWGQGTLVTVSS; I is the amino acid sequence (Seq. Id. No. 9) EIVLTQSPSSLSASLGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYA ASSSQSGVPSRFRGSESGTDFTLTISNLQPEDFATYYCQQSYRTPFTFGP GTKVETKR; and J is the amino acid sequence (Seq. Id. No. 10) VQLVQSGAEVKKPGASVKVSCTASGYSFTGYFLHWVRQAPGQGLEWMGRI NPDSGDTIYAQKFQDRVTLTRDTSIGTVYMELTSLTSDDTAVYYCARVPR GTYLDPWDYFDYWGQGTLVTVSS.