Patent ID: 8580537

Claim:
An isolated protein having transglutaminase activity, and comprising an amino acid sequence selected from the group consisting of: (A) the amino acid sequence of SEQ ID NO: 2, but having a mutation to cysteine at the 3-position and 283-position, and also having a mutation at a position selected from the group consisting of the 101-position, 157-position, 250-position, and combinations thereof; (B) the amino acid sequence selected from the group consisting of SEQ ID NO: 4, 6, 8, and 12, but having a mutation to cysteine at positions corresponding to positions in SEQ ID NO: 2 of the 3-position and 283-position, and also having a mutation at a position corresponding to a position in SEQ ID NO:2 selected from the group consisting of the 101-position, 157-position, 250-position, and combinations thereof; and (C) an amino acid sequence which is at least 95% homologous to the amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, 8, 10 and 12, but comprising a mutation at a position corresponding to a position in SEQ ID NO: 2 selected from the group consisting of the 101-position, 157-position, 250-position, and combinations thereof.