Patent ID: 8426578

Claim:
A composition for detecting ten or more nucleic acids of interest, the composition comprising: a single mixture, wherein the single mixture comprises: ten or more subsets of particles, a plurality of the particles in each subset being distinguishable from a plurality of the particles in every other subset, and the particles in each subset having associated therewith a different capture probe; ten or more subsets of n capture extenders, wherein n is at least two, wherein each subset of n capture extenders is capable of hybridizing to one of the nucleic acids of interest, and wherein the capture extenders in each subset are capable of hybridizing to one of the capture probes and thereby associating each subset of n capture extenders with a selected subset of the particles; wherein the capture probes and the capture extenders comprise only naturally occurring bases A, C, G, T, and/or U; ten or more subsets of one or more label extenders; and a label probe system comprising a label.