Patent ID: 7125488

Claim:
A composition for use as a stationary phase in chromatography comprising an inorganic substrate that is modified with at least one silane having the formula R 1 δ -Q α -(CH 2 ) β SiR 2 γ X 3-γ , wherein R 1 is hydrogen, C 1 –C 100 substituted or unsubstituted hydrocarbyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein the substituents are selected from C 1 –C 12 hydrocarbyl, hydroxyl, alkoxy, halogen, amino, nitro, sulfo, and carbonyl; α is 0 or 1; β is 0–30; γ is 0, 1 or 2; δ is 0–3; R 2 is C 1 –C 100 substituted or unsubstituted hydrocarbyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl; wherein the substituents are selected from C 1 –C 12 hydrocarbyl, hydroxyl, alkoxy, halogen, amino, nitro, sulfo, and carbonyl; Q is independently selected from —NHC(O)—, —C(O)NH—, —OC(O)NH—, —NHC(O)O—, —NHC(O)NH—, —NCO, —CHOHCHOH—, CH 2 OCHCH 2 O—, —(CH 2 CH 2 O) n —, —(CH 2 CH 2 CH 2 O) n —, —C(O)—, —C(O)O—, —OC(O)—, CH 3 C(O)CH 2 —, —S—, —SS—, —CHOH—, —O—, —SO—, —SO 2 —, —SO 3 —, —OSO 3 —, —SO 2 NH—, —SO 2 NMe—, —NH—, —NMe—, —NMe 2 + —, —N[(CH 2 ) n ] 2 + —, —CN, —NC, —CHOCH—, —NHC(NH)NH—, —NO 2 , —NO, —OPO 3 —, where n is 1–30; and X is a leaving group. wherein said inorganic substrate is equilibrated in an atmosphere having a defined relative humidity of less than 40% prior to being modified with the at least one silane, sufficiently to minimize anion exchange activity and enhance stability to base and acid treatment.