Patent ID: 7943299

Claim:
A method for determining the presence of at least one single nucleotide polymorphism at a nucleotide position in a first nucleotide sequence comprising providing a microarray having a plurality of second nucleotide sequences fixed at a predetermined site on said microarray, wherein each of said second nucleotide sequences is complementary to the first nucleotide sequence except for a single substitution of a different nucleotide at a position corresponding to the nucleotide position of the single nucleotide polymorphism in the first nucleotide sequence and wherein each of said second nucleotide sequences is between 15-30 nucleotides in length; hybridization of nucleotides of the first nucleotide sequence with said plurality of complementary second nucleotide sequences of the microarray, wherein said hybridization is performed in the presence of a p53 polypeptide and at a temperature of about 37° C.; and determining the presence of hybridization between nucleotides of said first nucleotide sequence and said plurality of complementary second nucleotide sequences of the microarray to indicate the presence of said at least one single nucleotide polymorphism.