Patent ID: 7396467

Claim:
A method of preparing a chromatography column, which method comprises the steps of (a) providing an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; (b) providing essentially spherical droplets of the polysaccharide solution; (c) forming a gel of the essentially spherical droplets; (d) cross-linking the gel by adding a cross-linking agent to form a porous cross-linked polysaccharide chromatography matrix; and (e) packing said porous cross-linked polysaccharide chromatography matrix in a chromatography column; wherein said porous cross-linked polysaccharide chromatography matrix allows higher flow rate of said column than matrix made from non-substituted polysaccharide.