Patent ID: 7722865

Claim:
A purified preparation of recombinant human N-acetylgalactosamine-6-sulfatase (GALNS) enzyme, said GALNS enzyme comprising an amino acid sequence at least 95% identical to amino acids 27 to 522 of SEQ ID NO: 4, and (a) having a purity of at least about 95% as determined by Coomassie Blue staining when subjected to SDS-PAGE under non-reducing conditions, (b) having at least about 90% conversion of the cysteine residue at position 53 to C α -formylglycine (FGly), and (c) having between 0.5 to 0.8 bis-phosphorylated oligomannose chains per monomeric protein chain, wherein at least 70% of said GALNS enzyme is in a precursor form as determined by Coomassie Blue staining when subjected to SDS-PAGE under reducing conditions.