Patent ID: 8106203

Claim:
A composition for reacting with a peroxidase enzyme to produce chemiluminescence which comprises: an aqueous buffer solution having a pH in the range of 5-10.5; a peroxide; and a compound of formula I wherein R 1 and R 2 are independently selected from alkyl, substituted alkyl, aryl, substituted aryl, aralkyl and substituted aralkyl groups of 1-20 carbon atoms and wherein R 1 and R 2 can be joined together to form a ring, wherein R 3 is selected from alkyl, substituted alkyl, aryl, substituted aryl, aralkyl and substituted aralkyl groups, wherein each of R 4 to R 11 is independently selected from hydrogen, alkyl, alkoxy, halogen, amino, or amino substituted with one or two alkyl or aryl groups, and wherein pairs of adjacent groups can be joined together as a carbocyclic or heterocyclic ring system comprising at least one 5 or 6-membered fused ring.