Patent ID: 7575885

Claim:
A method of determining whether a candidate compound is a modulator of tyrosylprotein sulfotransferase activity comprising: (a) contacting a candidate compound with a purified or recombinant tyrosylprotein sulfotransferase under conditions suitable for causing transfer of sulfate from a sulfate donor to a tyrosine of a protein, polypeptide or peptide, wherein said tyrosylprotein sulfotransferase is encoded by a polynucleotide selected from the group consisting of: (i) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 82-1194 of SEQ ID NO:2; (ii) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 289-1086 of SEQ ID NO:2; (iii) a polynucleotide encoding a polypeptide comprising the amino acid sequence encoded by nucleotides 82-1194 of SEQ ID NO:2; and (iv) a polynucleotide encoding a polypeptide comprising the amino acid sequence encoded by nucleotides 289-1086 of SEQ ID NO:2, and wherein the polynucleotides of (i) and (ii) hybridize under hybridization conditions comprising: incubation at 68° C. overnight followed by washing twice with two times SSC, 0.1% SDS at 22° C., and washing twice with 0.1 times SSC, 0.1% SDS at 50° C.; and (b) determining whether said candidate compound modulates the activity of said tyrosylprotein sulfotransferase.