Patent ID: 7329515

Claim:
A method for the synthesis of 3′-amino oligonucleotides comprising the steps of: (a) providing a solid support, wherein said solid support is selected from the group of compounds having the same structure: wherein R1, R2, R3 and R4 are independent selected from the group consisting of H, alkyl with up to 4 carbon atoms, heteroalkyl with up to 4 carbon atoms, phenyl, alkoxy with up to 4 carbon atoms, heteroalkoxy with up to 4 carbon atoms, carboxy, alkyloxycarbonyl with up to 4 carbon atoms in the alkyl chain, alkylcarbamoyl with up to 4 carbon atoms in the alkyl chain, halo, cyano, nitro, sulfo and alkylsulfonyl with up to 4 carbon atoms in the alkyl chain; R 5 is H or an acid labile protective group that is removed in the first deblocking step of a solid phase oligonucleotide synthesis; and Y is an organic spacer group comprising a straight or branched chain of one or more methylene groups, wherein the chain is optionally interrupted by one or more moieties independently selected from the group consisting of oxygen atoms, carbonyl groups, amide groups, ureido groups, urethane groups and aryl groups; (b) synthesizing an oligonucleotide pursuant to standard techniques for solid phase oligonucleotide synthesis (SPOS) wherein the oligonucleotide chain is assembled on the solid support provided in step (a); (c) cleaving the oligonucleotide from the solid support, and, (d) deprotecting the oligonucleotide compound completely, or deprotecting the oligonucleotide compound completely except for the 5′ terminal end protective group.