Patent ID: 7435587

Claim:
A method of growing cells under static or variable pressure comprising steps of: a. obtaining an amount of cells; b. determining the interstitial fluid pressure range experienced by said cells under in vivo conditions; c. seeding said cells in a fluid growth media within an apparatus comprising (i) a cell culture cassette comprising a cassette frame; two side walls secured to each side of the cassette frame to form a cell growing chamber; and ports for adjusting fluid pressure or flow, for providing culture media or cell culture, or for sampling cell culture or culture media within the cell growing chamber; (ii) an adapter comprising two side assemblies with each side assembly comprising a frame with a rigid support that fits over the cell culture cassette, wherein said side assemblies are attached at one end and positioned over the two side walls of the cell culture cassette; and (iii) a means to secure the two side assemblies over the two side walls of the cell culture cassette; d. applying static or dynamic hydrodynamic pressure via the ports to the cell culture cassette within said interstitial fluid pressure range; and e. providing an amount of nutrient supply to the cell culture cassette to support cell growth or proliferation.