Patent ID: 7341857

Claim:
An isolated esterase obtained from Ideonella sp. 0-0013 strain (FERM BP-08660) having the following properties: (1) Function, substrate specificity: hydrolyzes methyl 3-hydroxypalmitate to generate 3-hydroxypalmitic acid and methanol; (2) Optimal temperature for functioning: 37° C.; (3) Optimal pH and stable pH range: pH 7 or more to pH 10 or less; (4) Temperature stability: 97% of the enzyme is stable at 43° C.; (5) Inhibition, activation, and stabilization: Activated by sodium ion and potassium ion, and inhibited by strontium ion, iron ion (divalent), and methyl palmitate; (6) Molecular weight: About 46,500 Da (by SDS-PAGE), about 41,000 Da (by a gel filtration method); and (7) Isoelectric point: pI 4 (by polyacrylamide gel isoelectric focusing method).