Patent ID: 8486673

Claim:
A method of 1,3-propanediol production using crude glycerol, a by-product from biodiesel production, comprising: (a) inoculating a 1,3-propanediol-producing bacterial strain of Klebsiella pneumoniae in a seed culture medium containing the crude glycerol, (b) adding the resultant seed culture to a fermentation medium containing the crude glycerol, and fermenting under aerobic conditions at 30° C. to 37° C. to produce 1,3-propanediol, wherein a mixture of the crude glycerol and glucose is fed into the fermentation medium and the concentration of the glycerol in the fermentation medium is maintained at 10 to 40 g/L, wherein the crude glycerol from biodiesel production is added to the media in step (a) and in step (b) without any pretreatment, and (c) isolating and purifying 1,3-propanediol from fermentation medium.