Patent ID: 7816090

Claim:
A method of analyzing a sample for one or more analytes of interest, comprising: providing an element having a base layer; a layer containing streptavidin; and a spreading layer, wherein the streptavidin may or may not be in the spreading layer; providing immunocomponents and labeled immunoreactants which may be in the spreading layer or may be combined with the sample; spotting the sample onto the spreading layer at three or more areas, and optionally the immunocomponents and a labeled immunoreactant, wherein each center of the three or more areas is substantially equidistant to the center formed by the three or more areas, and wherein each area contacts an adjacent area such that wash fluid flow in any direction will contact sample; washing the sample and the labeled immunoreactant by directing a wash fluid at the center formed by the three or more areas, whereby the wash fluid flows away from the center towards the periphery of the element and equally flows over each of the three or more areas; and taking a measurement at each of the three or more areas to determine the presence or concentration of the one or more analytes.