Patent ID: 8334123

Claim:
A bacillus composition comprising 10 5 to 10 12 CFU/g of isolated bacillus spore cells, wherein (i) the bacillus spore cells are reach a vegetative cell growth point of 0.4 OD 630 after less than 18 hours and 19 hours in the presence of a 4 mM bile salt medium and a 6 mM bile salt medium, respectively, to produce bacillus vegetative cells; and (ii) the bacillus vegetative cells produce phytase in an amount of at least 1.25 times more than bacillus cell DSM 19467 after incubating for 4 hours at 37° C. in a non-selective heart infusion broth, and wherein each of the 4 mM bile salt medium and the 6 mM bile salt medium comprises non-selective veal infusion broth medium supplemented with a bile salt mixture comprising 60% taurodeoxycholate, 30% glycodeoxycholate and 10% deoxycholate.