Patent ID: 8445292

Claim:
A method of rapid fluorescent labeling of N-glycans for analysis, said method comprising: contacting a compound of formula I: with N-glycans under conditions sufficient to form N-glycans labeled with a moiety: wherein: the wavy line indicates the point of attachment to the rest of the molecule; each Y is independently O═ or S═; Z is —O— or —S—; either the subscript n=1-4 and each R 1 is independently selected from the group consisting of C 1-8 alkyl, C 1-8 heteroalkyl and aryl, and wherein adjacent R 1 groups together with the benzene ring to which they are attached form a fused carbocyclic aromatic ring system selected from the group consisting of naphthalene, phenanthrene, anthracene, triphenylene and pyrene, each of which having from 1-4 R 5 substituents selected from the group consisting of —COOH, —COO 31 M + , sulfo, alkylsulfo, phosphono and alkylphosphono; or n is zero; each R 2 is independently selected from the group consisting of —CO 2 R a , —CN, —NO 2 , —N 3 , —N═C═O, —N═C═S, —NO, —N═C═NR a , —S (O)R a , —S(O) 2 R a , —S(O) 2 NR a R b , —C(O)NR a R b , —C(O)R a , —C(O)SR a , —C(═S)R a , —S—CN, sulfo, phosphono, alkylphosphono, and alkylsulfo, wherein R a and R b are each independently selected from the group consisting of —H, alkyl, aryl, arylalkyl, cycloalkyl, heteroalkyl and haloalkyl or optionally R a and R b when attached to the same nitrogen atom are combined to form a 5-or 6-membered ring having from 0-2 additional heteroatoms as ring members selected from O, N and S; R 3 is —H or C 1-8 alkyl; each R 4 is independently C 1-8 alkyl; the subscript m is an integer from 1-5; the subscript p is 1 or 2; and the subscript q is zero or an integer from 1-4.