Patent ID: 7951333

Claim:
A microfluidic device comprising: a body of revolution having a rotation center and a circumference; a microfluidic structure disposed in the body of revolution comprising a plurality of chambers, channels connecting the chambers to each other and forming a path of fluid flow between the chambers, and valves disposed in the channels to control the fluid flow, the microfluidic structures transmitting fluid using centrifugal force due to rotation of the body of revolution, wherein one of the plurality of chambers receives a fluid biological sample; beads having a first probe to capture a target molecule from a fluid biological sample, the first probe selectively binds to the target molecule; and a fluid containing a second probe selectively binding to the target molecule and including a substance emitting an optical signal, wherein the microfluidic structure comprises: a sample chamber which contains a sample solution containing the fluid biological sample; a buffer chamber which contains a buffer solution; a mixing chamber which is fluid connected to the sample chamber, the buffer chamber through the channel; which receives a solution containing the second probe and a bead solution containing the beads; which comprises an inlet to receive a fluid and an outlet to discharge the fluid, the outlet being disposed with a greater distance from the rotation center of the body of revolution than the inlet, and the outlet being provided with a valve to control a flow of the fluid discharged from the mixing chamber; and in which the sample and the beads are brought into contact with each other and the second probe to produce a bead-target molecule-second probe complex, if the fluid biological sample contains the target molecule; and separates the bead-target molecule-second probe complex; a waste chamber which is fluid connected to the outlet of the mixing chamber through a channel, the waste chamber receiving the fluid discharged from the mixing chamber by changes of phases of a valve; and an optical signal emission chamber connected to the outlet of the mixing chamber through a channel, in which a substrate solution is brought into contact with the bead-target molecule-second probe complex to generate an optical signal.