Patent ID: 7524639

Claim:
A method for the ex vivo determination of a level of severity of extrapyramidal symptoms caused by a compound in a subject, the compound having unknown extrapyramidal side effects in the subject to be treated with the compound, the method comprising: (a) applying the compound to a non-neuronal cell line that expresses a 5-HT 2A receptor; (b) determining a pattern of differential internalization of the receptor in the cell line, wherein the pattern of internalization is classified into: (i) Type A, which exhibits internalization of the receptor to a recycling endosome in the cell line and loss of the receptor from a cell surface with the internalized receptor being localized to the recycling endosome; (ii) Type B, which exhibits internalization of the receptor to the recycling endosome in the cell line and loss of the receptor from the cell surface along with appearance of the receptor in puncta within the cell line; and (iii) Type C, which exhibits no visible internalization of the receptor in the cell line, such that Type A predicts low extrapyramidal symptoms, Type B predicts moderate extrapyramidal symptoms and Type C predicts high extrapyramidal symptoms, wherein the compound is an antipsychotic drug.