Patent ID: 8822185

Claim:
A method of increasing the weight percent of at least one polyunsaturated fatty acid in the total fatty acids of an oleaginous Yarrowia , comprising: a) providing an oleaginous Yarrowia comprising: 1) genes encoding a functional polyunsaturated fatty acid biosynthetic pathway, said genes comprising at least one gene encoding an enzyme selected from the group consisting of delta-15 desaturase, delta-6 desaturase, and delta-9 elongase; and 2) a disruption in a native gene encoding a peroxisome biogenesis factor 3 protein, wherein the disruption comprises a gene knockout comprising a nucleotide sequence having at least 95% identity with sequence 1-862 of SEQ ID NO:82; and b) growing said Yarrowia under conditions as to increase the weight percent of at least one polyunsaturated fatty acid in the total fatty acids of said Yarrowia , wherein said increase is with respect to the weight percent of the at least one polyunsaturated fatty acid in the total fatty acids of the Yarrowia in which no native gene encoding a PEX protein has been disrupted.