Patent ID: 7172894

Claim:
An isolated gene comprising a DNA sequence that encodes a polypeptide having an amino acid sequence selected from the group consisting of: (a) SEQ ID NO: 1; (b) a polypeptide having 95% or more sequence homology to SEQ ID NO: 1, and having at least the ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol; (c) a polypeptide encoded by DNA of SEQ ID NO: 2; (d) a polypeptide encoded by DNA having 95% or more homology to SEQ ID NO: 2, and having at least the ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol; (e) a polypeptide encoded by DNA that hybridizes under stringent conditions with the complementary sequence to SEQ ID NO: 2, the stringent conditions comprising conducting the hybridization in a solution containing 50% formamide under a high ion concentration of 6Ã—SSC at 65Â° C., and then washing under a low ion concentration of 0.1Ã—SSC at 65Â° C. wherein the polypeptide has at least the ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol; (f) a polypeptide having 90% or more sequence homology with SEQ ID NO:1, wherein the polypeptide has at least the ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol and wherein the polypeptide is obtained from a microorganism belonging to the genus Leifsonia ; and (g) a polypeptide having 90% or more sequince homology with SEQ ID NO: 1, wherein the polypeptide has at least the ability to reduce 2,2,2-trifluoroacetophenone to 2,2,2-trifluoro-1-phenylethanol and wherein the polypeptide is obtained from Leifsonia sp. S-749.