Patent ID: 7888123

Claim:
A method for introducing a transgene into chromosomal DNA of a cell of Labyrinthulomycota by homologous recombination comprising, (a) introducing into a cell of Labyrinthulomycota a recombinant vector comprising at least: (1) a polynucleotide, wherein the polynucleotide has the polynucleotide sequence set forth as SEQ ID NO: 1, the polynucleotide is homologous to a part of chromosomal DNA in the cell of Labyrinthulomycota, and the polynucleotide undergoes homologous recombination with the part of the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream of the selection marker gene, and (3) a transgene having a promoter sequence located upstream and a terminator sequence located downstream of the transgene, (b) selecting a cell of Labyrinthulomycota expressing the selection marker gene.