Patent ID: 8241866

Claim:
A kit for use in a method for preparing a measurement sample for detecting live cells, injured cells and dead cells of a microorganism in a test sample by flow cytometry, which comprises the following elements: an enzyme selected from lipolytic enzymes and proteases, which has an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, a topoisomerase poison selected from the group consisting or amsacrine, camptothecin, doxorubicin, ellipticine, etoposide, mitoxantrone, saintopin, topotecan CP-115,953 and ethidium monoazide and/or a DNA gyrase poison selected from the group consisting of ciprofloxacin, ofloxacin, enoxacin, pefloxacin, fleroxacin, norfloxacin, nalidixic acid, oxolinic acid and piromidic acid, and a first stain agent which stains live cells in different color from injured cells and dead cells, and a second stain agent which stains live cells and injured cells in different color from dead cells.