Patent ID: 6864062

Claim:
A method for detecting autoantibodies that are present in Alzheimer's disease comprising: (a) obtaining a protein preparation consisting essentially of an antigen that is immunologically reactive with a monoclonal antibody produced by the hybridoma cell line identified as ATCC No. HB9205, said preparation being substantially free of immunoglobulin G, a bovine microtubule associated protein preparation, and a sample being tested for the presence of said autoantibodies; (b) electrophoresing said protein preparation and said bovine microtubule associated protein preparation on separate lanes on a gel; (c) transferring said electrophoresed protein preparation and said bovine microtubule associated protein preparation to a membrane; (d) contacting said membrane with a sample being tested for the presence of said autoantibodies such that an autoantibody complex can form with antigen present in said protein preparation and/or with antigen present in said bovine microtubule associated protein preparation; and (e) detecting said autoantibodies by the formation of said complex(es).