Patent ID: 8540983

Claim:
A debriding composition obtained from bromelain, the debriding composition comprising a plurality of different proteolytic enzymes having molecular weights of about 23 kDa as determined by size exclusion chromatography, wherein the plurality of different proteolytic enzymes comprises: (a) stem bromelain comprising SEQ ID. No. 1; (b) ananain comprising SEQ ID No. 2; (c) stem bromelain comprising SEQ ID No. 3; and (d) cysteine proteinase precursor comprising SEQ ID No. 4, said debriding composition comprising bromelain inhibitors in an amount of less than 10% w/w of protein content of the debriding composition, wherein the composition consists essentially of a single protein peak after elution from an HPLC size exclusion column TSK-Gel 3000 SWXL , the single protein peak constituting proteins having molecular weights of about 23 kDa.