Patent ID: 8574432

Claim:
An integrated microfluidic liquid chromatography device comprising: a substrate having a surface; at least two separation columns each having an inlet and an outlet, the at least two separation columns integrated into the substrate surface and adapted to perform separate and simultaneous chromatographic separations on at least two different samples, the chromatographic separations being of different types, at least one of the separation columns formed by depositing, using chemical vapor deposition (CVD), a polymer over neighboring etched regions of the substrate, one etched region being narrower and one etched region being wider, the depositing sufficient to coat a portion of a bottom of and seal over the narrower etched region and define an inner channel and then coat an inner surface of the inner channel from the polymer diffusing through the wider region until the wider etched region is sealed over by the deposition; and a detector array comprising at least two sample detectors integrated into the substrate surface and in fluid communication with the outlet of the at least two separation columns.