Patent ID: 8226986

Claim:
A method for treating disease, injury or a disorder in a biological material comprising contacting the biological material with an effective amount of a sterile, stable aqueous pharmaceutical composition comprising: a solution of deoxygenated water and sodium sulfide; wherein the sodium sulfide produces Na2S, H2S and HS—; wherein the concentration of H2S is in the range of 1.0 mg/mL to 15.0 mg/mL; wherein the solution is adjusted to a pH in the range of 7.5 to 8.5 by adding HCl to the solution; wherein the solution has an osmolarity in the range of 250-330 mOsmol/L; wherein the solution further comprises sulfide oxidation products selected from the group consisting of polysulfide, sulfite, sulfate and thiosulfate; wherein the oxidation products comprise sulfate in the range of 0%-1.0%, sulfite in the range of 0%-1.0%, polysulfide in the range of 0%-1% or thiosulfate in the range of 0%-1.0%; and wherein the solution is isotonic or near isotonic; and wherein the disease, injury or disorder can be treated with liquid or gaseous chalcogenide compositions; is a surgery; is risk for reperfusion injury; is an ischemic or hypoxic condition arising from stroke, cardiac arrest, myocardial infarction, or coronary artery bypass graft (CABG) surgery; is hemorrhagic shock, or; is hepatic injury.