Patent ID: 7592420

Claim:
A composition comprising a protease inhibitor selected from the group consisting of: a Bowman-Birk inhibitor (BBI) comprising SEQ ID NO:7 wherein residue 13 is Ala or is changed to Ser, Gly or Gln, and wherein residues 15-21 and/or 42-48 have been replaced with a variant sequence; and a Soybean Trypsin Inhibitor (STI) comprising SEQ ID NO:12 wherein residues 40-85 and/or 137-144 have been replaced with a variant sequence; wherein said variant sequence is selected from the group consisting of: a VEGF binding peptide consisting of YNLYGWT (SEQ ID NO:77); a complement protein c2 binding peptide consisting of GRKIPIQ (SEQ ID NO:78); a complement protein c3 binding peptide consisting of ARSNLDE (SEQ ID NO:79); a complement protein c4 binding peptide consisting of QRALPIL (SEQ ID NO:80); a complement protein c5 binding peptide consisting of GRLHMKT (SEQ ID NO:81); a complement factor B binding peptide consisting of KRKIVLD (SEQ ID NO:82); a membrane metalloprotease 2 binding peptide consisting of AAMFGPA (SEQ ID NO:83); a membrane metalloprotease 12 binding peptide consisting of GALGLFG (SEQ ID NO:84); a cotton binding peptide consisting of EPLIHQR (SEQ ID NO:85); and a cotton binding peptide consisting of SAFRGPT (SEQ ID NO:86), wherein said protease inhibitor is produced in a filamentous fungal cell according to a method comprising, a) introducing a DNA construct into a filamentous fungal cell, wherein said DNA construct comprises a promoter showing transcription activity in the filamentous fungal cell and which is operably linked to a heterologous DNA sequence encoding the protease inhibitor, b) culturing the filamentous fungal cell under suitable culture conditions to allow expression of the heterologous DNA sequence, and c) producing the protease inhibitor.