Patent ID: 7329796

Claim:
A method for preparing an ungulate animal, said method comprising: a) forming a first embryo by nuclear transfer of an ungulate cell obtained from a cell culture of a differentiated cell in the G1 stage of the cell cycle, or a nucleus thereof, into a first enucleated metaphase II-arrested oocyte obtained from the same species as the ungulate cell and activating the reconstituted oocyte; b) forming a second embryo by nuclear transfer of a cell obtained from said first embryo, or a nucleus thereof, into a second enucleated oocyte obtained from the same species as the cell in step (a) wherein said cell obtained from said first embryo is not expanded in culture; and c) transferring said second embryo into the uterus of an ungulate of the same species as the cell in step (a) so as to produce a fetus that undergoes full fetal development and parturition to generate said ungulate animal.