Patent ID: 8426152

Claim:
A method of detecting changes in the refractive index at the surface of a localized surface plasmon resonance (LSPR) detection system, the method comprising: a) generating an insoluble product from an enzymatic substrate using an immobilized enzyme, wherein the insoluble product accumulates on a LSPR supporting surface, and the enzyme is immobilized by attachment to an antibody; and b) detecting changes in the refractive index via the reflected or transmitted light of the surface arising from the presence of the insoluble product using LSPR, wherein the surface comprises nanoparticles, wherein each nanoparticle is from about 5 nm to about 1000 nm in size, and each nanoparticle comprises a transition metal selected from the group consisting of Au, Ag, Ta, Pt, Pd, Rh and Cu, and glass or a polymer selected from the group consisting of polystyrene, polymethacrylate and polyacrylate.