Patent ID: 7186383

Claim:
An apparatus, comprising: a fluidic cartridge including a first substrate, a second substrate and a flexible intermediate interlayer, wherein said flexible intermediate interlayer is sealedly interfaced between said first substrate and said second substrate to form therein a plurality of channels of capillary dimensions; at least an open reservoir, a first pump chamber, and a reaction chamber formed at least partially in said first substrate or said second substrate of said fluidic cartridge, wherein said open reservoir and said reaction chamber are each connected to said first pump chamber through channels in said plurality of channels and wherein said open reservoir and said reaction chamber are in fluid communication with each other via a channel in said plurality of channels; a fluid flow controlling structure, formed in said fluidic cartridge, restricting a flow of a fluid through said reaction chamber via said plurality of channels to one direction only; and a linear actuator providing a pumping action in said first pump chamber to pump said fluid to recirculate from said reaction chamber to said open reservoir via said first pump chamber and said plurality of channels.