Patent ID: 7323347

Claim:
A process of fabricating a sensing surface, comprising the steps of: (a) forming a base layer, said base layer is composed of a monolayer of colloidal particles chosen from a group consisting of proteins, peptides, colloidal PTFE particles, colloidal polyethylene particles, colloidal polypropylene particles wherein said particles are of a diameter in the range of 0.5 nm to 100 nm; (b) epoxy-group activating said monolayer by covalent coupling of a monolayer of a bisoxirane, said epoxy-group activated monolayer is of a thickness not to exceed 1 nm; (c) reacting a fraction of said epoxy groups with thioglycolate to incorporate carboxyl groups and the remaining fraction are hydrolyzed to yield hydroxyl groups; and (d) binding ligands to said monolayer.