Patent ID: 8148170

Claim:
An apparatus comprising: (a) a solid substrate with an exposed surface; (b) a compound provided as a layer, bound to the solid substrate; and (c) a plurality of recombinant single chain antibodies (scFv's) specific for a target molecule and bound to the compound on the solid substrate, each scFv comprising: (i) an antibody variable light chain (V L ) polypeptide specific for the target molecule, (ii) an antibody variable heavy chain (V H ) polypeptide specific for the target molecule, and (iii) a linker polypeptide covalently linking the antibody variable light chain (V L ) polypeptide to the antibody variable heavy chain (V H ) polypeptide, the linker polypeptide having an amino acid sequence comprising one or more amino acids with sidechains that bind to the compound, wherein the scFv is capable of binding the target molecule to the solid substrate, when provided to the apparatus.