Patent ID: 8384899

Claim:
A method of detecting optical signals, comprising: irradiating a first area of a substrate with a first excitation source that emits a first excitation wavelength or wavelength range and simultaneously irradiating a second area of the substrate that is spatially offset from the first area of the substrate with a second excitation source that emits a second excitation wavelength or wavelength range, the first excitation wavelength or wavelength range different from the second excitation wavelength or wavelength range; and detecting a first emission wavelength or wavelength range emitted from the first area of the substrate by a first camera of a TDI detection system, and simultaneously detecting a second emission wavelength or wavelength range emitted from the second area of the substrate by a second camera of the TDI detection system, the first emission wavelength or wavelength range corresponding to a first nucleotide present in the first area of the substrate and the second emission wavelength range corresponding to a second nucleotide present in the second area of the substrate, the first nucleotide being different from the second nucleotide.