Patent ID: 8304387

Claim:
A lyophilized composition comprising a keratinocyte growth factor (KGF), histidine, mannitol, sucrose, and polysorbate 20, wherein reconstitution of the lyophilized composition with water yields a reconstituted solution having a pH of about 6.5 and comprising about 10 mM histidine, about 4% mannitol, about 2% sucrose, and about 0.01% (w/v) polysorbate 20, and wherein the KGF stimulates keratinocyte proliferation and comprises the amino acid sequence of SEQ ID NO: 2 or a variant thereof selected from the group consisting of SEQ ID NO: 3 (ΔN23), ΔN15, ΔN16, ΔN18, ΔN24, ΔN25, ΔN26, ΔN27, C(1,15)S, ΔN3/C(15)S, ΔN3/C(15)-, ΔN8/C(15)S, ΔN8/C(15)-, C(1,15)S/R(144)E, C(I,15)S/R(144)Q, ΔN23/R(144)Q, C(I,15,40)S, C(1,15,102)S, C(I, 15,102,106)S, ΔN23/N(137)E, ΔN23/K(139)E, ΔN23/K(139)Q, ΔN23/R(144)A, ΔN23/R(144)E, ΔN23/R(144)L, ΔN23/K(147)E, ΔN23/K(147)Q, ΔN23/K(153)E, ΔN23/K(153)Q, ΔN23/Q(152)E/K(153)E, R(144)Q, and H(116)G.