Patent ID: 7118923

Claim:
A method for detecting a selected analyte in test solution, comprising providing a sensor comprising: a test vessel; a semipermeable membrane having pores for retaining the analyte, wherein said semipermeable membrane divides said test vessel into a first volume and a second volume, wherein said pores are selected to prevent the analyte from passing into or through said membrane, wherein said membrane is chemically modified by attachment of membrane surface modifiers on at least a side facing said first volume but not within said pores, wherein said membrane supports a 100 kPa pressure load, said membrane is functionalized with a binder at the surface of said membrane in order for said membrane to act as a sensor; assay labels, disposed within said first volume, said assay labels having label modifiers, said label modifiers having a binding affinity for the membrane modifiers in the presence of the analyte, and a measurably different binding affinity for said membrane modifiers in the absence of analyte; a pressure source, for driving said test solution from said first volume into said second volume through said modified surface of said membrane contacting the test solution with said membrane and; removing labels not bound to said analyte and; detecting the presence or absence of said analyte by detecting the presence or absence of said labels on said membrane.