Patent ID: 8466410

Claim:
A method for measuring a fluorescent light amount, whereby a specimen is irradiated with excitation light, and fluorescent light generated from the specimen is measured by a light-receiving element via a receiving optical system, said method comprising: a step of acquiring a relationship between a light-amount value of the fluorescent light generated from the specimen per unit area and an output value of the light-receiving element; a step of irradiating the specimen with excitation light having a light-amount value per specified unit area traceable to a national standard, and designated on a surface of the specimen beforehand; a step of acquiring the output value of the light-receiving element when the specimen is irradiated with the excitation light by the step of irradiation step; and a step of computing an absolute value of a ratio of a light-amount of the excitation light per unit area with which the specimen is irradiated to the light-amount value of fluorescent light generated from the specimen per specified unit area, or the number of molecules contained in the specimen per unit area, based on the relationship between the output value of the light-receiving element acquired by the step of acquiring the output value, and the relationship acquired by the step of acquire a relationship, and the light-amount value per specified unit area.