Patent ID: 8389699

Claim:
A kit for sequencing a polynucleotide sequence, the kit comprising: (a) a cationically charged nucleic acid terminator, the cationically charged nucleic acid terminator comprising a labeled compound of structure (I): Z—X—S—B-L (I) wherein Z is selected from the group consisting of a mono-phosphate, a di-phosphate, a tri-phosphate, a thiophosphate, and a boranophosphate; X is selected from the group consisting of O, CH 2 , S, and NH; S is selected from the group consisting of a sugar and a sugar analogue; B is selected from the group consisting of a naturally occurring base, a synthetic base, and a nucleobase; L is a linker that is selected from the group consisting of alkyl, alkenyl, and alkynyl; wherein L is substituted with a cationic moiety comprising more than one charged units that are covalently linked together which imparts a positive charge to structure (I), and wherein at least one of L, B, S, X, or Z is substituted with a reporter moiety; each of the charged units is an imidazolium moiety; and (b) a discriminatory polymerase that is exonuclease minus.