Patent ID: 8431355

Claim:
A process for screening glycoform specific antibodies capable of binding to at least one given glycoform of a second glycoprotein among antibodies elicited against a first glycoprotein, said first glycoprotein being pituitary or blood human TSH from healthy subjects, said second glycoprotein being a recombinant human TSH produced by mammalian cells and said second glycoprotein being itself a glycoform of the first glycoprotein, said process comprising the following steps: checking that a panel of antibodies elicited against the first glycoprotein bind to said recombinant human TSH, said antibodies being classified in pools, each pool being characterized in that two antibodies selected from the same pool can not bind to the same glycoprotein at the same time, (1) contacting said panel of antibodies elicited against the first glycoprotein with at least one glycoform of said recombinant human TSH, (2) determining the binding affinity between said antibodies and the first glycoprotein, or said recombinant human TSH, or at least one glycoform of said recombinant human TSH, and recovering antibodies recognizing said recombinant human TSH, or at least one glycoform of said recombinant human TSH with a higher affinity than that displayed with the first glycoprotein, wherein said glycoform of said recombinant human TSH is selected from a group of glycoforms of said recombinant human TSH, each glycoform of said group corresponding to a determined glycosylation state being: a) essentially more sialylated, more branched and less fucosylated than the recombinant human TSH, or b) essentially more sialylated, less branched and less fucosylated than the recombinant human TSH.