Patent ID: 7816129

Claim:
A recombinant expression vector for production of bacterial enzyme in a Trichoderma host, the vector comprising: (a) a promoter functional in said Trichoderma host and operably linked to a first DNA sequence, wherein said first DNA sequence encodes a signal sequence functional in said Trichoderma host and a functional domain or functional domains of the native amino acid sequence of Trichoderma reesei mannanase I (MANI), Trichoderma reesei cellobiohydrolase I (CBHI), Trichoderma reesei cellobiohydrolase II (CBHII), Trichoderma reesei endoglucanase I (EGI), or Trichoderma reesei endoglucanase II (EGII), wherein said functional domain or domains of said MANI consist of the native T. reesei amino acid sequence of: (1) the core domain of said MANI, (2) said MANI core domain and the hinge domain of said MANI, (3) the tail domain of said MANI, or (4) said MANI tail domain and said MANI hinge domain; wherein said functional domain or said domains of said CBHI consist of the native T. reesei amino acid sequence of: (1) the tail domain of said CBHI, or (2) the tail and hinge domains of said CBHI, wherein said functional domain or domains of said CBHII consist of the native T. reesei amino acid sequence of: (1) the core domain of said CBHII, (2) the core and hinge domains of said CBHII, (3) the tail domain of said CBHII, or (4) the tail and hinge domain of said CBHII, wherein said functional domain or domains of said EGI consist of the native T. reesei amino acid sequence of: (1) the core domain of said EGI, (2) the core and hinge domains of said EGI, (3) the tail domain of said EGI, or (4) the tail and hinge domain of said EGI; wherein said functional domain or domains of said EGII consist of the native T reesei amino acid sequence of: (1) the core domain of said EGII, (2) the core and hinge domains of said EGII, (3) the tail domain of said EGII, or (4) the tail and hinge domain of said EGII, and (b) a second DNA sequence which is fused in frame to said first DNA sequence, wherein said second DNA sequence encodes a bacterial enzyme wherein said domain or domains result in said bacterial enzyme being secreted from said host at levels that are higher than the levels achieved under the same conditions but using a recombinant expression vector that lacks said DNA sequence encoding said functional domain or domains.