Patent ID: 7521226

Claim:
A diagnostic kit for detecting an amine or a hydrolytic enzyme within a test sample, the kit comprising: a plurality of reactive complexes that each comprises a substrate joined to a reporter and a specific binding member, said substrate being cleavable by a hydrolytic enzyme to release said reporter; and a chromatographic medium for the receipt of said reactive complexes, reporters, or both, said chromatographic medium defining a first enzyme detection zone within which is immobilized a receptive material that has an affinity for said specific binding member for generating an enzyme detection signal, wherein the presence or quantity of a hydrolytic enzyme is determinable from said enzyme detection signal, said chromatographic medium further defining an amine detection zone positioned downstream from said first enzyme detection zone, wherein a chemichromic dye is contained within said amine detection zone, said chemichromic dye being capable of undergoing a color change in the presence of an amine, wherein the presence or quantity of an amine is determinable from said color change.