Patent ID: 6946586

Claim:
A method for screening for overexpression of a transcription factor polynucleotide in plants, said method comprising: (a) providing a first pool of donor vectors, wherein each donor vector member comprises a LexA DNA binding domain fused to a GAL4 transcription activation domain, and a second pool of receptor vectors, wherein each receptor vector member comprises a LexA DNA binding site operably linked to a transcription factor polynucleotide; (b) transforming a first plant with a member of said donor vector pool; (c) transforming a second plant with a member of said receptor vector pool; (d) crossing said first and second transformed plants to generate a hybrid plant, wherein the hybrid plant comprises the member of said donor vector pool and the member of said receptor vector pool; and (e) characterizing the phenotype of said hybrid plant so as to identify a hybrid plant overexpressing the transcription factor polynucleotide.