Patent ID: 8349336

Claim:
An isolated antigenic S. pyogenes polypeptide or peptide consisting of a) amino acids 1-48, 22-46, 22-410, 25-48, 49-70, or 181-223, of SEQ ID NO: 166, or a polypeptide consisting of at least 95% sequence identity to amino acids 1-48, 22-46, 22-410, 25-48, 49-70, or 181-223 of SEQ ID NO: 166, or a peptide consisting of amino acids 4-20, 80-89, 96-103, 105-119, 123-129, 153-160, 227-233, 236-243, 248-255, 261-269, 274-279, 283-299, 305-313, 315-332, 339-344, 349-362, 365-373, 380-388, 391-397, or 402-407 of SEQ ID NO: 166, or b) amino acids 22-410 of SEQ ID NO: 166 with one or more amino acid substitutions selected from A214D, Y3095, T317N, V318C and K319Q, 181-223 of SEQ ID NO: 166 with an A214D amino acid substitution, 305-313 of SEQ ID NO: 166 with a Y309S amino acid substitution, or 315-332 of SEQ ID NO: 166 with one or more amino acid substitutions selected from T317N, V318C and K319Q.