Patent ID: 8377900

Claim:
A method for regulating the expression of a target gene in a host cell, said method comprising the steps of: (a) introducing into said host cell an expression vector comprising: a nucleic acid molecule, the nucleic acid molecule encoding a biologically active chimeric transactivator protein comprising: (i) a functional DNA binding domain of a bacteriophage λ repressor protein comprising amino acids 1-131 thereof (SEQ ID NO.: 8) and further comprising an amino acid mutation at at least one position selected from V92 and S93, such that the binding domain is not capable of dimerization; (ii) bacterial DNA gyrase B subunit (Gyr B); and (iii) a transcription activation domain; wherein the nucleic acid molecule is operatively linked to an expression control sequence; and a target gene, wherein said target gene is operatively linked to at least an operator DNA sequence that is recognized by the DNA binding domain of the λ repressor protein; (b) allowing expression of said biologically active chimeric transactivator encoded by said expression vector; (c) adding an effective amount of coumermycin and novobiocin into said cell, wherein addition of coumermycin to said cell produces dimerization of the chimeric transactivator protein, which then binds to the operator DNA sequence and activates expression of said target gene product, and addition of novobiocin inhibits coumermycin-induced dimerization of the chimeric transactivator protein.