Patent ID: 8168172

Claim:
A process for the production of organic formulation of biopesticide containing Pseudomonas fluorescens comprising: a first step of preparing a Mother culture of P. fluorescens and inoculating in King's B broth, at 30±1° C. for 24-36 hours; a second step of liquid fermentation of the culture obtained through inoculating the Mother culture in King's B broth in a first substrate comprising 5-10% pongamia cake aqueous extract, 5-10% neem cake aqueous extract, 0.3-0.5% sugarcane molasses and King's B broth; followed by a third step of solid fermentation of the culture obtained through liquid fermentation in a second substrate comprising sterile Pongamia deoiled cake, Neem deoiled cake, and Wheat Bran.