Patent ID: 6929939

Claim:
A subtilisin variant comprising three or more amino acid substitutions selected from the group of substitution sets consisting of: (a) the replacement isoleucine at position 122 with alanine, the replacement of isoleucine at position 79 with alanine, and the replacement of tyrosine at position 217 with any of leucine, methionine or serine; and (b) the replacement of isoleucine at position 122 with alanine, the replacement of asparagine at position 76 with aspartic acid, the replacement of isoleucine at position 79 with alanine, and the replacement of tyrosine at position 217 with any of leucine, methionine or serine; wherein, (i) said positions 76, 79, 122, and 217 correspond to positions 76, 79, 122, and 217 in the amino acid sequence of Bacillus amyloliquefaciens subtilisin set forth in SEQ ID NO:3, and (ii) the subtilisin variant produces a lesser immunogenic response by comparison with a subtilisin not comprising a substitution set of (a) or (b) measured by an in vitro reduction in allergenicity.