Patent ID: 8062871

Claim:
A method for producing adipyl-CoA, comprising culturing a host microbial organism in which at least one exogenous nucleic acid is introduced under conditions and for a sufficient period of time to produce adipyl-CoA, wherein said host microbial organism comprises at least one exogenous nucleic acid encoding a set of adipyl-CoA pathway enzymes that are expressed in a sufficient amount to produce adipvl-CoA, wherein said set of adipyl-CoA pathway enzymes convert succinyl-CoA and acetyl-CoA to 3-oxoadipyl-CoA, 3-oxoadipyl-CoA to 3-hydroxyadipyl-CoA, 3-hydroxyadipyl-CoA to 5-carboxy-2pentenoyl-CoA and 5-carboxy-2-pentenoyl-CoA to adipyl-CoA.