Patent ID: 8137928

Claim:
A method of producing a soluble truncated active eukaryotic glycosyltransferase in a prokaryotic microorganism, which method comprising the steps of (a) providing a prokaryotic microorganism that has an oxidizing intracellular environment, (b) expressing a nucleic acid that encodes a truncated eukaryotic glycosyltransferase in the prokaryotic microorganism, wherein the truncated eukaryotic glycosyltransferase is a truncated human GalNacT2 of SEQ ID NO: 33 having a truncation selected from the group consisting of Δ51 Δ445, or one or two truncations selected from the group consisting of Δ40, Δ53, Δ73, Δ94, and Δ445, and (c) growing the microorganism under conditions that allow expression of the soluble truncated active eukaryotic glycosyltransferase within a cellular compartment of the prokaryotic microorganism.