Patent ID: 7799533

Claim:
A method for screening for a compound to change binding of a polypeptide to a receptor, wherein the receptor comprises the amino acid sequence of SEQ ID NO: 3, and wherein the polypeptide comprises a partial sequence of an amino acid sequence selected from the group consisting of SEQ ID NO: 15, 38, 40, and 42, and wherein the method comprises at least one method selected from the group consisting of: (A) (i) contacting a labeled form of the polypeptide with said receptor protein in the presence or absence of a test compound; (ii) measuring the level of the binding of the labeled polypeptide to the receptor protein in both the presence and absence of the test compound; and (iii) making a comparison of the level of the binding of the peptide in the presence of the test compound and in the absence of the test compound; (B) (i) contacting a labeled form of the polypeptide with a cell containing said receptor protein or a membrane fraction of the cell containing said receptor protein in the presence or absence of a test compound; (ii) measuring the level of the binding of the labeled polypeptide to the receptor protein in both the presence and absence of the test compound; and (iii) making a comparison of the level of the binding of the peptide in the presence of the test compound and in the absence of the test compound; and (C) (i) contacting a labeled form of the polypeptide with the receptor protein expressed on a cell membrane during cultivation of a transformant harboring the DNA encoding the receptor protein in the presence and absence of a test compound; (ii) measuring the level of the binding of the labeled polypeptide to the receptor protein in both the presence and absence of the test compound; and (iii) making a comparison of the level of the binding of the peptide in the presence of the test compound and in the absence of the test compound.