Patent ID: 8236355

Claim:
A method for inhibiting COX-2 activity, said method comprising contacting a cell expressing COX-2 with an effective amount of a COX-2 inhibitor comprising a mixture of denatured plasma proteins and at least one metal ion or metal salt thereof, wherein the COX-2 inhibitor is manufactured by: (a) mixing plasma with sodium bicarbonate (NaHCO 3 ) and trypsin incubating said mixture for sufficient time and at a temperature of no more than 80° C. to produce a precipitate of denatured plasma proteins; (b) resolubilising said precipitate in the presence of an aqueous solution at a temperature of between about 80° C. and about 150° C., wherein either before, during or after the resolubilising step at least one metal ion or metal salt thereof is admixed; and (c) separating the COX-2 inhibitor from the resolubilised precipitate in step (b), which COX-2 inhibitor comprises denatured plasma proteins and the at least one metal ion or metal salt thereof.