Patent ID: 6890728

Claim:
A method of making an autologous platelet gel containing a transfected gene, said method comprising: a) providing a centrifuge container for receiving and separating a first autologous anticoagulated blood sample; b) rotating said centrifuge container; c) providing a first aliquot of said first anticoagulated whole blood sample from a patient to whom said gel is to be applied to said rotating centrifuge container, whereby said whole blood separates radially into an outermost first fraction containing red blood cells, a second fraction comprising a buffy coat containing white blood cells, a third fraction comprising platelets, and a fourth fraction containing platelet poor plasma; d) withdrawing said buffy coat fraction from said rotating centrifuge container; e) transfecting said white blood cells in said isolated buffy coat fraction with a vector/gene combination; and f) combining said buffy coat fraction containing said transfected white blood cells with autologous thrombin and an autologous blood component.