Patent ID: 7741020

Claim:
An improved Lp-PLA 2 enzyme assay method for plasma or serum sample obtained from a patient treated orally with an Lp-PLA 2 inhibitor, wherein the improvement provides for detecting about 85% or greater inhibition of the enzyme by the Lp-PLA 2 inhibitor, the method comprising: (a) contacting 110 μL of a solution comprising: a solution comprising 90 mM 1-myristoryl-2-(4-nitrophenylsuccinyl) phosphatidylcholine contacted with a solution comprising 200 mM HEPES, 200 mM NaCl, 5 mM EDTA, 10 mM CHAPS, 10 mM sodium 1-nonanesulfonate at a pH 7.6 in a ratio of 0.66 μL to 110 μL; with at least one 25 μL plasma or serum sample from an animal; with 25 μL each of a p-nitrophenol standard solution comprising; 4, 3, 2, 1, 0.4 or 0.2 nmol/μL p-nitrophenol in methanol; and (b) preparing a blank comprised of 25 μL of phosphate buffered saline or H 2 O; and (c) determining Lp-PLA 2 activity using a colormetric measurement.