Patent ID: 8058004

Claim:
A process for creating a mixture of oligonucleotide sequences in solution comprising: (a) synthesizing in situ or spotting a plurality of oligonucleotide sequences on a microarray device or bead device each having a solid or porous surface, wherein the plurality of oligonucleotide sequences are attached to the solid or porous surface and wherein each oligonucleotide sequence comprises a fragment of a target polynucleotide sequence and further comprises two flanking sequences, one at the 3â€² end and the other at the 5â€² end of each fragment, wherein each flanking sequence is from about 7 to about 50 bases and comprising a primer region and a sequence segment having a restriction enzyme cleavable site; (b) amplifying each oligonucleotide sequence using primers complementary to the primer regions of the flanking sequence to form a plurality of double stranded oligonucleotide sequences; and (c) cleaving the primer regions from the plurality of double stranded oligonucleotide sequences at the restriction enzyme cleavable sites, thereby to produce a plurality of fragments of the target polynucleotide sequence, wherein the plurality of fragments together comprise the target polynucleotide sequence.