Patent ID: 8436170

Claim:
A compound of Formula Ia or Ib: wherein: X is O, S, or —C(CH 3 ) 2 —; A is —OR 1 or —NR 1 R 1a ; B is ═O or ═N + R 4 R 4a ; C is —OR 4 or —NR 4 R 4a ; R 1 R 1a , R 4 and R 4a are each independently H or alkyl, unsubstituted or substituted with -L-SO 3 − , -L-PO 3 2− , a water-soluble polymer, or with -L-R x ; or one or more pair of R 1 and R 1a or R 4 and R 4a together with the nitrogen to which they are attached form a saturated or unsaturated ring optionally substituted by any of -L-SO 3 − , -L-PO 3 2− and -L-R x ; or at least one of R 1 , R 1a , R 4 , or R 4a is an enzyme substrate or a protecting group; R 2 , R 3 , R 5 , R 6 , R 7 , R 8 and R 9 are each independently H, halogen, CN, alkyl, alkenyl, alkoxy, alkylthio, alkylamino, dialkylamino, alkylaminocarbonyl, dialkylaminocarbonyl, aryl, heteroaryl, -L-PO 3 2− , L-SO 3 − , -L-R x , a reactive sulfonamide or an unreactive sulfonamide; or one or more pair of R 2 and R 1a , R 3 and R 4a , R 4 and R 5 , R 5 and R 6 , R 8 and R 9 , or R 9 and R 1 , together with the atoms to which they are attached form one or more fused saturated or unsaturated rings that are optionally substituted by at least one halogen, CN, alkyl, heteroalkyl, alkenyl, alkoxy, alkylthio, alkylamino, dialkylamino, alkylaminocarbonyl, dialkylaminocarbonyl, aryl, heteroaryl, -L-PO 3 2− , L-SO 3 − , -L-R x , a reactive sulfonamide or an unreactive sulfonamide; R 7 ′ is H, OH, CN, or C 1 -C 6 alkoxy; or R 7 ′ in combination with R 7 forms a 5- or 6-membered spirolactone or spirosultone ring; R x is a reactive group; L is a bond or (Q) n ; each Q is independently NR d , S(O) t , O, C(═X′), (C═X′), substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, substituted or unsubstituted cycloalkyl, or substituted or unsubstituted heterocycloalkyl, wherein t is 0-2, and no more than two NR d are adjacent and no two O are adjacent; each X′ is independently NR d , S or O; n is 1-20; each R d is H, substituted or unsubstituted alkyl; provided that at least one of R 2 , R 3 , R 5 and R 9 is a reactive sulfonamide, wherein one or more pair of R 2 and R 1a , R 3 and R 4a , R 4 and R 5 , R 5 and R 6 , R 8 and R 9 , or R 9 and R 1 , together with the atoms to which they are attached form one or more fused saturated or unsaturated rings that are optionally substituted by at least one halogen, CN, alkyl, heteroalkyl, alkenyl, alkoxy, alkylthio, alkylamino, dialkylamino, alkylaminocarbonyl, dialkylaminocarbonyl, aryl, heteroaryl, -L-PO 3 2− , -L-SO 3 − , -L-R x , a reactive sulfonamide or an unreactive sulfonamide.