Patent ID: 8512978

Claim:
A method of treating a slide having a biological tissue deposited thereon to replace oil on the slide with water, the method comprising; (a) contacting the oil on the slide with an exchanging composition, wherein the exchanging composition consists essentially of a surfactant dissolved in a non-polar organic solvent thereby forming a microemulsion; and (b) rinsing the microemulsion from the slide with water, wherein the surfactant has the following structural formula: R 1 —X—(CH 2 CH 2 O) n —R 2 , wherein R 1 is selected from the group consisting of a hydrocarbon from about C4 to about C20, a polypropylene oxide, a polysiloxane, and a fluoroalkane; R 2 is selected from the group consisting of a hydrocarbon from about C1 to C20, an alkyl carboxylic acid, an alkyl sulfonate, an alkyl amine, an alkyl amine oxide, an alkyl quaternary amine, a polypropylene oxide, a polysiloxane, a fluoroalkane and a hydrogen; X is a linking group comprising an ether, ester, carbonate, benzyl, or sorbitol; and n is from about 5 to about 30.