Patent ID: 8784799

Claim:
An adeno-associated virus (AAV) particle comprising: an AAV capsid; and a vector genome comprising in the 5′ to 3′ direction: (i) a 5′ AAV terminal repeat; (ii) a first heterologous nucleotide sequence; (iii) an AAV inverted terminal repeat that forms a hairpin structure, does not comprise a functional AAV terminal resolution site (trs), and is resolved by Rep protein to a lesser extent as compared with the 5′ AAV terminal repeat and a 3′ AAV terminal repeat; (iv) a separate heterologous nucleotide sequence; and (v) the 3′ AAV terminal repeat; wherein the first and the separate heterologous nucleotide sequences are essentially self-complementary and form a double-stranded nucleic acid sequence that encodes a factor IX polypeptide.