Patent ID: 8017355

Claim:
A method for expressing a recombinant polypeptide in a Pseudomonas fluorescens host cell comprising: a) providing a Pseudomonas fluorescens host cell; b) transforming the Pseudomonas fluorescens host cell with at least one nucleic acid construct encoding a recombinant polypeptide operably linked to a mannitol-inducible promoter comprising the promoter of an endogenous Pseudomonas fluorescens mtlEFGKDYZ operon; c) growing the Pseudomonas fluorescens host cell in a growth medium; d) adding a carbon source to the growth medium; e) expressing the recombinant polypeptide of interest by inducing the mannitol-inducible promoter; and, f) isolating the expressed recombinant polypeptide; wherein the Pseudomonas fluorescens host cell has a mutation or deletion of a gene selected from the group consisting of mtlD, mtlY, and mtlZ, or a combination thereof.