Patent ID: 7833522

Claim:
A method to treat a patient having a wound area, comprising the steps of: providing a plurality of epidermal and/or dermal cells, wherein said epidermal and/or dermal cells comprise a cell diameter; providing a spraying apparatus comprising a gas inlet, a barrel interconnected with said gas inlet, and reservoir assembly interconnected with said barrel; wherein said reservoir assembly further comprises a container; a first tubular member comprising a proximal portion disposed within said container and a distal portion extending outwardly from said container; a needle valve interconnected with said distal end of said first tubular member, wherein said needle valve is formed to include a first orifice comprising a first diameter between about 50 to about 100 times said cell diameter; a second tubular member having a proximal portion and a distal portion, wherein said needle valve is disposed within said proximal portion of said second tubular member, and wherein said distal portion of said second tubular member is disposed within said barrel, and wherein said distal end of said second tubular member is formed to comprise a second orifice comprising a second diameter between about 100 to 250 times said cell diameter; preparing a therapeutic mixture comprising said epidermal and/or dermal cells and a liquid carrier disposing said therapeutic mixture in said reservoir assembly; interconnecting a source of pressurized gas to said gas inlet, wherein said pressurized gas comprises a pressure of between about 10 to about 44 psi at said gas inlet; directing pressurized gas through said barrel; mixing said therapeutic mixture and said pressurized gas in said barrel; emitting from said barrel a spray comprising said pressurized gas and said therapeutic mixture; and directing said spray onto said wound area to deposit a layer of epidermal and/or dermal cells thereon.