Patent ID: 8497080

Claim:
A method for determining an EGFR signaling profile of a tissue section, comprising the steps of: (a) staining the tissue section with: (i) a reagent which detects EGFR; (ii) a second reagent which detects an EGFR effector molecule; and (iii) a third reagent which detects a subcellular compartment; (b) obtaining a high resolution digital image of: (i) the EGFR; (ii) the EGFR effector molecule; and (iii) the subcellular compartment; and (c) analyzing the digital image so obtained to obtain an amount of: (i) the EGFR; (ii) the EGFR effector molecule present in the subcellular compartment; and (iii) the EGFR effector molecule present outside the subcellular compartment; (d) determining a ratio of the amount obtained in step c(ii) to the amount obtained in step c(iii); (e) comparing the EGFR amount obtained in step c(i) to an expression level of EGFR in a control specimen; (f) comparing the ratio determined in step (d) to a corresponding ratio obtained for the control specimen; thereby determining the EGFR signaling profile.