Patent ID: 7741459

Claim:
A solid phase comprising a surface first chemical moiety which participates in covalent carboxyl bond formation with a second chemical moiety conjugated to a tag oligonucleotide rendered partially double stranded by annealing an α-tag oligonucleotide to the tag oligonucleotide to provide a 3′ overhang portion of the tag oligonucleotide wherein the tag oligonucleotide is employed as a substrate for ligase-mediated covalent bonding to a single-stranded target nucleic acid molecule, such that the single-stranded target nucleic acid molecule is ligated to the tag oligonucleotide, wherein the tag oligonucleotide comprises the second chemical moiety conjugated to a known oligonucleotide sequence via a molecule comprising mc+n atoms, from about 1 to about 100, wherein m is the number of repeats of size c and n is the number of atoms not included in the repeats.