Patent ID: 8273562

Claim:
An isolated DNA selected from the group consisting of: (a) a DNA comprising the nucleotide sequence of SEQ ID No: 1; (b) a DNA that hybridizes under stringent conditions with a DNA comprising a nucleotide sequence complementary to the nucleotide sequence of SEQ ID No: 1, and wherein said stringent conditions comprise washing at a salt concentration of 0.1×SSC and 0.1% SDS at 37° C., and wherein said DNA encodes a protein having L-isoleucine dioxygenase activity; (c) a DNA that encodes a protein comprising the amino acid sequence of SEQ ID No: 2; (d) a DNA that encodes a protein comprising the amino acid sequence of SEQ ID No: 2, but which includes one to 13 substitutions, deletions, insertions, additions, or inversions of one to 13 amino acids and wherein said protein has L-isoleucine dioxygenase activity; and (e) a DNA that encodes a protein comprising an amino acid sequence that is at least 98% homologous to the amino acid sequence of SEQ ID NO: 2, and has L-isoleucine dioxygenase activity.