Patent ID: 7323320

Claim:
A process for assembling a polynucleotide from a plurality of different oligonucleotides comprising: (a) providing the plurality of different oligonucleotides that together comprise the polynucleotide sequence, wherein each of said oligonucleotides has an overlapping sequence region corresponding to a sequence region in a next oligonucleotide, and wherein a plurality of the different oligonucleotides further comprises flanking sequence regions at the 3′ and 5′ ends thereof, wherein said flanking sequence regions comprise primer binding sites that are the same for each oligonucleotide and a sequence segment having a restriction enzyme recognition sequence for removing the primer binding sites; (b) amplifying each oligonucleotide using a pair of primers complementary to the primer binding sites of the flanking sequence regions; (c) cleaving the oligonucleotides to remove the primer binding sites; and (d) assembling the cleaved oligonucleotides through the overlapping sequence regions to form a full length polynucleotide.