Patent ID: 7264934

Claim:
A method for parallel sequencing of a plurality of nucleic acid templates, comprising: a) immobilizing a plurality of primed nucleic acid templates on a support structure, wherein each primed template includes a template and a primer, and is localized to an identifiable, discrete location on said support structure; b) initiating a plurality of complexation reactions on said support structure, by forming a reaction mixture, said reaction mixture including said plurality of primed templates, a nucleic acid polymerizing enzyme, and at least one nucleotide to form a plurality of closed complexes, each comprising one of said plurality of primed templates, said nucleic acid polymerizing enzyme, and one of said at least one nucleotide, wherein said nucleotide contains a base that complements the template base at the site of polymerization; c) removing unbound nucleotide and other unbound components of said reaction mixture; d) adding a divalent cation to complete the reaction; e) detecting, at each of said identifiable, discrete locations, incorporated nucleotide or other reaction product for each closed complex; f) removing said divalent cation and other end products; and g) repeating steps b) through f) for the sequencing of each additional nucleotide of said plurality of primed templates.