Patent ID: 7923213

Claim:
A method of detecting an analyte in a sample comprising: (a) contacting said sample with a solid support and an activator compound conjugate, wherein said solid support comprises a chemiluminescent compound and a first specific binding partner for the analyte, wherein said chemiluminescent compound comprises a chemiluminescent acridan moiety and a linking moiety that links the chemiluminescent acridan moiety to the solid support, and said activator compound conjugate comprises a peroxidase enzyme conjugated to a second specific binding partner for the analyte; (b) allowing the analyte to bind to said first specific binding partner and to said second specific binding partner thereby forming a bound activator compound conjugate; (c) after step (b), contacting said bound activator compound conjugate with a trigger solution comprising a peroxide and an enhancer compound thereby activating said chemiluminescent compound and producing chemiluminescence; (d) detecting the chemiluminescence produced in step (c) thereby detecting the analyte.