Patent ID: 8496579

Claim:
A method for generating high-resolution microscopic images in the microendoscopy based on laser-induced object reaction radiation, comprising the following steps: focusing pulsed excitation radiation from a laser system into an object by means of a transmission focusing optics unit comprising a transmission system and miniature focusing optics having a high numerical aperture greater than 0.55 which are rigidly connected to the end of the transmission system to trigger a local reaction radiation of the object in the micrometer to nanometer range; executing a scanning movement by the distal end of the transmission focusing optics unit in at least two dimensions for the successive spatially changed position of the excitation radiation relative to the object and for locally allocated receiving high resolution object reaction radiation according to the locally changed position of the object present at the distal end of the miniature focusing optics; and transmitting the object reaction radiation which is scanned in a locally progressive manner by the transmission focusing optics unit to an image-generating system with a photon detectors wherein a third scanning process is carried out orthogonal to the two-dimensional scanning movement by axial movement of the transmission focusing optics unit for changing the depth of an image recording of the object reaction radiation generated from the two-dimensional scanning movement into a focal plane predetermined by the miniature focusing optics.