Patent ID: 7575888

Claim:
A method of measuring the biological activity of a human chymase, comprising the steps of: a. contacting the human chymase with a human Secretory Leukocyte Protease Inhibitor in an assay mixture; b. incubating the assay mixture under a condition wherein the human chymase cleaves the human Secretory Leukocyte Protease Inhibitor; and c. measuring the level of human Secretory Leukocyte Protease Inhibitor cleavage by human chymase in the assay mixture, wherein said level of human Secretory Leukocyte Protease Inhibitor cleavage by chymase is measured as a ratio of human Secretory Leukocyte Protease Inhibitor fragments resulting from chymase cleavage to the full length Secretory Leukocyte Protease Inhibitor present in the assay mixture; and d. correlating said level of human Secretory Leukocyte Protease Inhibitor cleavage to said biological activity of said human chymase.