Patent ID: 7067267

Claim:
A method for isolating pyrrole-containing biological compounds from a sample, the method comprising: 1) contacting the sample with a biotinylated Ehrlich's reagent wherein the biotinylated Ehrlich's reagent comprises a cleavable bond attached through the amine moiety of Ehrlich's reagent and the cleavable bond links the Ehrlich's reagent to biotin such that a reaction product is formed between the pyrrole-containing biological compound and the biotinylated Ehrlich's reagent; 2) contacting the reaction product with avidin or streptavidin attached to a solid support, such that the reaction product is bound to the solid support through biotin-avidin/streptavidin binding; and 3) isolating the reaction product bound to the support by cleaving the cleavable bond of the Ehrlich's reagent.