Patent ID: 7872243

Claim:
Method for detecting an energy transfer between a donor fluorescent compound and an acceptor fluorescent compound present in a measurement medium, comprising the following steps: (i) exciting the measurement medium by a light beam at a wavelength λ 1 , wherein λ 1 is the wavelength at which said donor fluorescent compound is excited, (ii) measuring the signal resulting from the fluorescence emitted at a wavelength λ 3 , wherein λ 3 is the wavelength at which the fluorescence of the acceptor fluorescent compound is emitted, (iii) measuring the signal resulting from the fluorescence emitted at a wavelength λ 2 , wherein λ 2 is the wavelength at which the fluorescence of the donor fluorescent compound is emitted, and (iv) correcting the signal resulting from the fluorescence emitted by the acceptor fluorescent compound at the wavelength λ 3 by the signal resulting from the fluorescence emitted by the donor fluorescent compound at the wavelength λ 2 , wherein the donor fluorescent compound and the acceptor fluorescent compound are different, the exciting light beam is polarized, and the signal resulting from the fluorescence emitted at the wavelength λ 3 is measured in a plane different from the polarization plane of the exciting light.