Patent ID: 8709801

Claim:
A kit for the capture of circulating tumor cells in a body fluid sample or a serum-containing sample, comprising: (a) a solid support; (b) endothelium or epithelium cells that are attached to the solid support; (c) white blood cells for capturing the circulating tumor cells in the body fluid sample or the serum-containing sample, which are attached on the cells of (b) or placed in a separate container; and (d) one or more inflammation-inducing agents, wherein the one or more inflammation-inducing agents induce an inflammation reaction on said attached endothelium or epithelium cells of (b) and white blood cells of (c); whereby upon adding the body fluid sample or serum-containing sample to the solid support the white-blood cells of (c) and the endothelium or epithelium cells of (b) capture circulating tumor cells contained in the body fluid sample or the serum-containing sample; and wherein the white blood cells are neutrophils, eosinophils, basophils, lymphocytes, monocytes or macrophages, wherein the one or more inflammation-inducing agent are tumor necrosis factors or interleukins, and wherein the body fluid sample or the serum-containing sample is pre-diluted more than 10 times.