Patent ID: 7462701

Claim:
A method for production and purification of a soluble heterologous fusion protein comprising a cellulose binding module (CBM), from transgenic plants or transgenic plant cells expressing said fusion protein, comprising (a) disrupting the transgenic plant material; (b) adding an extraction liquid to the plant material, thereby creating a mixture of soluble and insoluble plant material, so as to extract the soluble fusion protein from said disrupted plant material to the liquid phase to obtain a protein extract; (c) separating the insoluble plant material, comprising cell-wall material and solids, from said protein extract comprising said fusion protein of interest; (d) contacting said protein extract to a polysaccharide matrix which binds to said fusion protein; (e) washing the matrix with the bound fusion protein with one or more suitable aqueous solutions; and (f) eluting the fusion protein from said polysaccharide matrix by adjusting conditions effecting the release of said fusion protein from the matrix, thereby obtaining the soluble heterologous fusion protein.