Patent ID: 8344103

Claim:
A method for preparation of a peptide by liquid phase synthesis, comprising providing a solvent system, the solvent system comprising a first solvent or mixed solvent A and a second solvent or mixed solvent B, wherein the solvent system can change phase states reversibly in response to a change in temperature between a first phase state, which is a homogenously compatibilized mixed solvent system in which the first solvent or mixed solvent A and the second solvent or mixed solvent B are homogenously compatibilized and mixed, and a second phase state, in which the first solvent or mixed solvent A is present in a separate phase from the second solvent or mixed solvent B such that the solvent system is separated into two or more phases, combining the solvent system with a compound comprising a carrier, the compound having solubility in the first solvent or mixed solvent A of the solvent system, dissolving a compound comprising one or more amino acids in the first solvent or mixed solvent A, in the second solvent or mixed solvent B, or in said solvent system in the first phase state, forming said solvent system in the first phase state that is homogenously compatibilized by placing the solvent system at a first temperature, extending said compound comprising one or more amino acids by introducing an amino acid to the carboxy end of said compound to form a peptide product, wherein the solvent system is preferentially at a second temperature lower than the first temperature and wherein the solvent system at the second temperature remains in the first phase state that is homogenously compatibilized, changing the temperature condition so that the solvent system transitions from the first phase state to the second phase state, in which the first solvent or mixed solvent A is present in a separate phase from the second solvent or mixed solvent B, and recovering the peptide product from one of the separate phases.