Patent ID: 8305579

Claim:
A method of probing multiple targets in a sample comprising: (a) providing the sample containing the multiple targets; (b) binding at least one probe comprising a binder coupled to an enzyme to one or more target present in the sample; (c) reacting the bound probe in step (b) with an enzyme substrate coupled to a fluorescent signal generator; (d) observing a signal from the fluorescent signal generator of step (c); (e) after observing the signal, inactivating both the fluorescent signal generator and the enzyme by applying a solution comprising an oxidizing agent; (f) binding at least one probe comprising a binder coupled to an enzyme to one or more target present in the sample, after step (e); (g) reacting the bound probe in step (f) with an enzyme substrate coupled to a fluorescent signal generator; (h) observing a signal from the fluorescent signal generator of step (g).