Patent ID: 8592176

Claim:
A method for forming a stratified structure of epithelial cells, comprising: (1) obtaining vascular endothelial cells, fibroblast cells and epithelial cells from a human organ, wherein the endothelial cells, the fibroblast cells and the epithelial cells are orthotopic with regard to the originating organ, and wherein the organ is not skin; (2) transplanting the vascular endothelial cells on the fibroblast cells, wherein the number of vascular endothelial cells is one-tenth of the number of fibroblast cells; (3) irradiating the combination of the vascular endothelial cells and the fibroblast cells with 20Gy or less X-ray or γ-ray to form a feeder layer; and (4) transplanting the epithelial cells on the feeder layer and culturing said epithelial cells thereon to form a stratified structure of the epithelial cells in the presence of at least one of collagen, elastin, proteoglycan, fibronectin, laminin and tenascin.