Patent ID: 8354244

Claim:
A method for detecting visceral Hemangiosarcoma in canine subjects comprising: obtaining a blood sample from a canine subject; obtaining a serum portion from said blood sample; providing a substrate having a 3′-derivative of thymidine; providing a phosphate donor for releasing a phosphate radical; incubating said serum portion in a solution comprising said substrate and said phosphate donor to cause a phosphorylation reaction resulting in the transfer of said phosphate radical from said phosphate donor to said substrate, producing a phosphorylated substrate; measuring a concentration of said phosphorylated substrate; determining a level of Thymidine kinase activity based on said concentration of said phosphorylated substrate; and determining said canine subject has Hemangiosarcoma with approximately 80% sensitivity if said level of activity of Thymidine kinase is higher than about 8 units per liter.