Patent ID: 8124379

Claim:
A method for producing a secreted polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of a polypeptide, wherein the host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence, the 3′ end of the first nucleotide sequence is immediately upstream of the second nucleotide sequence, and the first nucleotide sequence is selected from the group consisting of: (i) a nucleotide sequence encoding a signal peptide amino acid sequence which has at least 80% identity with SEQ ID NO:1 and no more than 18 amino acids and (ii) a nucleotide sequence having at least 90% identity with SEQ ID NO:2 and no more than 54 nucleotides; and (b) isolating the secreted polypeptide from the cultivation medium; wherein the signal peptide retains the ability to direct the polypeptide into or across a cell membrane.