Patent ID: 8497138

Claim:
A method for selecting a cell or cell colony which produces a polypeptide of interest, comprising: a) providing a medium comprising cells and a detection agent capable of binding to the polypeptide of interest; b) providing a solid phase having a capture agent disposed thereon, wherein the capture agent is capable of binding to the polypeptide of interest; c) contacting the medium with the solid phase having the capture agent disposed thereon; d) detecting a signal produced by the detection agent bound to the polypeptide which is captured by the capture agent on the solid phase, wherein the solid phase comprises a culture dish, a well or a plate; and e) selecting a cell or cell colony generating a level of the signal that indicates the cell or cell colony has produced the polypeptide of interest which has been captured adjacent the cell or cell colony by the capture agent disposed on the solid phase, and bound by the detection agent.