Patent ID: 8921809

Claim:
A microscopy device comprising: illumination means for generating an illumination beam; an imaging detector comprising a plurality of detector elements for spatially resolved acquisition of an fluorescence emission radiation emitted by an object to be examined and time-resolved acquisition of photons of the fluorescence emission radiation; an illumination beam path between the illumination means and the object to be examined; and a detection beam path between the object to be examined and the detector; wherein the illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path; wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined; wherein the microscopy device comprises evaluating and controlling means which are designed to discriminate between different signal components of the fluorescence emission radiation depending on an acquisition time of photons of the emission radiation; wherein the controlling and evaluating means are designed to carry out an imaging evaluation in parallel for at least two of the signal components; and wherein the illumination means comprise a pulsed laser.