Patent ID: 8420387

Claim:
A method of making an intein modified protein including: identifying a plurality of cysteine, serine or threonine sites within the protein, whether native or introduced; analyzing the local sequence of each cysteine, serine or threonine site in the plurality of cysteine, serine or threonine sites with a support vector machine; identifying an active site of the protein, wherein a respective distance separates the active site and each of the cysteine, serine or threonine sites in the plurality of cysteine, serine or threonine sites, and identifying the respective distance between the active site and each of the cysteine, serine or threonine sites in the plurality of cysteine, serine or threonine sites; and identifying a secondary structure of the protein at each of the cysteine, serine or threonine sites in the plurality of cysteine, serine or threonine sites; inserting an intein into the protein at a site immediately before one of the cysteine, serine or threonine sites in the plurality of cysteine, serine or threonine sites that is ranked 0.75 or higher by the support vector machine, within ten angstroms of the active site, and within ten amino acids from a loop-β-sheet junction or within ten amino acids from a loop-α-helix junction; and screening the intein modified protein to determine if the intein modified protein has a permissive or switching phenotype.