Patent ID: 7384761

Claim:
A method of screening for a bioactive agent capable of interfering with the dephosphorylation of Rad9, comprising: (a) combining: (i) a cell cycle protein PP5, wherein said PP5 protein comprises an amino acid sequence having at least about 95% identity to the amino acid sequence set forth in SEQ ID NO:2 and has protein phosphatase activity; (ii) a candidate bioactive agent, wherein the candidate bioactive agent is selected from the group consisting of a peptide, polynucleotide, a lipid, and a small organic molecule; (iii) and phosphorylated Rad9, wherein Rad9 is capable of being dephosphorylated by an active PP5; and (b) determining the phosphorylation state of Rad9, wherein decreased dephosphorylation of said Rad9 in the presence of said bioactive agent as compared with a control indicates the bioactive agent interferes with dephosphorylation of Rad9.