Patent ID: 8435730

Claim:
A method for freezing semen comprising: (a) introducing ejaculate comprising sperms cells into a semen extender composition to provide a cryogenic solution, the semen extender composition comprising: (i) about 0.1 wt. % to about 6 wt. % phospholipid obtained from a non-animal source; (ii) about 0.0001 wt. % to about 1 wt. % anionic surfactant comprising a sulfate; (iii) about 0.5 wt. % to about 3 wt. % carbohydrate; (iv) about 3 wt. % to about 14 wt. % freeze agent; and (v) biological buffer to provide the composition with a pH of about 6.9 to about 7.5, and wherein the composition comprises a sufficient amount of water so that the composition exhibits an osmolality of about 250 mOsM to about 350 mOsM, wherein the composition is substantially free of animal products; and (b) freezing the cryogenic solution to provide a frozen solution.