Patent ID: 8563276

Claim:
A method of enzymatic cleavage of polysaccharides comprising a first sequence [→4)-β-D-GlcpA-(1→4)-α-L-Rhap3 sulfate-(1→] n and a second sequence [→4)-α-L-IdopA-(1→4)-α-L-Rhap3 sulfate-(1→] m , the first and the second sequences being respectively constituted of two monosaccharide units joined by a glycoside bond, comprising: supplying a solution of ulvan polysaccharides having said first sequence and said second sequence; supplying a genus Ochrobactrum microorganism able to produce an enzymatic substance of the class of the lyases which are adapted to cause cleavage of the glycoside bond according to a reaction of β elimination, according to the schematic mechanism: said enzymatic substance having an enzymatic activity which is tolerant of an increase in degradation products in the solution; and contacting the enzymatic substance with the ulvan polysaccharides under such conditions that the enzymatic substance is effective to cause said cleavage substantially without loss of enzymatic activity due to a production of polysaccharide degradation products.