Patent ID: 8283130

Claim:
A method of detecting the presence of an antibody against Anaplasma phagocytophilum in a biological sample of a mammal comprising: (i) immobilizing an isolated polypeptide antigen onto a surface, wherein said immobilized isolated polypeptide antigen has the amino acid sequence set forth in SEQ ID NO: 13 or SEQ ID NO: 15; (ii) contacting said immobilized isolated polypeptide antigen with the biological sample of the mammal, under conditions that allow formation of an antibody-antigen complex, said biological sample containing an antibody against Anaplasma phagocytophilum , wherein said antibody binds to said immobilized isolated polypeptide antigen and forms a complex of the antibody and the polypeptide antigen; and (iii) detecting the formation of said antibody-polypeptide antigen complex, wherein said detected antibody-polypeptide antigen complex is indicative of the presence of said antibody against Anaplasma phagocytophilum in said biological sample.