Patent ID: 8877444

Claim:
A method for identifying the variety/line of a plant of the genus Saccharum , comprising a step of genotyping at least one simple sequence repeat selected from simple sequence repeat polymorphisms in DNA sequences of SEQ ID NOs: 1, 2, 3, 5, 6, 10, and 12, wherein the step of genotyping is carried out by the steps of: (a) carrying out PCR amplification using DNA extracted from a sugarcane to be identified as a template and a primer set consisting of a forward and a reverse primer that specifically amplify a region containing a simple sequence repeat in a DNA sequence of SEQ ID NOs: 1, 2, 3, 5, 6, 10, or 12; (b) determining the molecular weights of amplified DNA fragments; and (c) performing genotyping of the region containing a simple sequence repeat based on distribution of molecular weights.