Patent ID: 7625719

Claim:
A method for detecting chemotaxis of cells using a chemotactic detecting apparatus which comprises i) a first well and a second well which are connected to each other via a channel, ii) one or more tubes for injecting/sucking a sample which are provided for said first and second wells and iii) a space in common at the top ends of said tubes in which a liquid is held to maintain said liquid in said wells and said channel under a definite pressure, said method comprising the steps of: (a) filling up the whole apparatus with said liquid not affecting cells and chemoattractants; (b) injecting a cell suspension at said first well through said tube; (c) sucking an appropriate amount of said liquid through said tube of said second well located oppositely across the channel to adjust the position of the cells in said first well; (d) returning the level of said liquid to the original position by injecting said liquid; (e) injecting a chemotactic factor at said second well through said tube; and (f) detecting cells passing by own actions through said channel.