Patent ID: 8518641

Claim:
A method for locating DNA damage in a tissue sample comprising: 1) obtaining a sample of DNA from the tissue sample; 2) exposing said DNA to at least one selected DNA-damage binding factor which binds to DNA at a site of damage whereby a precise location of the damage can be located, which damage binding factor is selected from one of a DNA repair enzyme or an antibody which recognizes DNA damage and is tagged with or comprises a given binding molecule; 3) shearing the DNA to produce fragments thereof; 4) precipitating fragments that include, or are associated with the tagged DNA-damage binding factor by binding the said tag to a selected binding partner in order to isolate fragments of DNA that include, or are associated with, said DNA-damage binding factor; 5) optionally amplifying said precipitated fragments and then labeling said precipitated fragments; and 6) exposing said labeled precipitated fragments to a genome-wide microarray under conditions that enable the fragments to hybridize to the array whereby the location of any, or the, DNA damage can be determined having regard to the location of the hybridization fragments with respect to the array; wherein step 2) may be carried out either before or after step 3).