Patent ID: 8257949

Claim:
A self-priming poly-sialyltransferase fusion protein comprising a bi-functional sialyltransferase comprising an amino acid sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 8 and having α-2,3 and α-2,8 activity and a poly-sialyltransferase comprising an amino acid sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 3 and having α-2,8 and/or α-2,9 activity, wherein the self-priming poly-sialyltransferase fusion protein transfers sialic acid moieties from a donor substrate to an acceptor substrate to produce a poly-sialylated product and wherein the poly-sialylated product comprises an oligosaccharide that comprises at least three sialic acid moieties.