Patent ID: 8663941

Claim:
A method for diagnosing an ischemic state in myocardium of a subject showing signs and symptoms of acute coronary syndrome but not fulfilling the diagnostic criteria for a myocardial infarction of an amount of troponin T determined in a sample of the subject greater than 0.1 ng/ml, comprising: contacting a portion of a sample from a subject with an antibody having specific binding affinity for soluble fms-like tyrosine kinase-1 (sFLT-1), thereby forming a complex between the antibody and sFLT-1, the antibody having a detectable label; separating the complex formed in said step of contacting from antibody not comprising the complex; quantifying a signal from the detectable label of the antibody comprising the complex formed in said step of contacting, the signal being proportional to an amount of sFLT-1 in the sample, whereby an amount of sFLT-1 in the sample is calculated; comparing the amount of sFLT-1 determined in said step of quantifying to a sFLT-1 reference amount; and providing a diagnosis of an ischemic state in myocardium of the subject if the amount of sFLT-1 in the sample calculated in said step of quantifying is greater than the sFLT-1 reference amount.