Patent ID: 7435549

Claim:
A method of identifying at least one epitope binding domain capable of binding to a predetermined epitope comprising: (a) displaying on the surface of a biological display system a panel of recombinant polypeptides comprised of: (1) an N-terminal blocking domain at the N-terminus of said recombinant polypeptides, (2) a C-terminal anchoring domain at the C-terminus of said recombinant polypeptides, said C-terminal anchoring domain mediates anchoring of said recombinant polypeptides to the surface of said display system, and (3) at least one epitope binding domain positioned between said N-terminal blocking domain and said C-terminal anchoring domain; (b) exposing said recombinant polypeptides to said predetermined epitope; and (c) identifying a subset of said recombinant polypeptides that comprise an epitope binding domain capable of binding to said predetermined epitope.