Patent ID: 8377655

Claim:
A quantitative PCR assay which differentiates between expression level of type 1 insulin-like growth factor receptor (IGF-IR), insulin receptor isoform A (IR-A), or insulin receptor isoform B (IR-B) within rodent tissues or cells comprising (a) providing sense and antisense primer pairs specific to IGF-IR, wherein said primer pairs are set forth in SEQ ID NO:8 and SEQ ID NO:9; (b) providing sense and antisense primer pairs specific to IR-A, wherein said primer pairs are set forth in SEQ ID NO:5 and SEQ ID NO:6; (c) providing sense and antisense primer pairs specific to IR-B, wherein said primer pairs are set forth in SEQ ID NO:5 and SEQ ID NO:7; (d) performing amplification of IGF-IR, IR-A, and IR-B; and (e) measuring the amount of IGF-IR, IR-A, and IR-B obtained relative to each other.