Patent ID: 8361753

Claim:
A method of amplifying nucleic acids, said method comprising: amplifying nucleic acid using a modified oligonucleotide primer, wherein said modified oligonucleotide primer comprises a modification group at one or two internucleotide linkages; wherein said modification groups are at the n, n-1, n-2, or n-3 positions, wherein n is the 3′ terminal internucleotide linkage; wherein the presence of said modification group impairs nucleic acid polymerase extension of said modified oligonucleotide primer; wherein said modification group can be non-reversibly thermally dissociated; and wherein said modified oligonucleotide primer has structure I as follows wherein: Nuc is a nucleoside within the primer sequence; U and Z are independently O, S, Se, NR 9 , or CR 9 R 10 ; R 9 and R 10 are each independently hydrogen or optionally substituted straight or branched hydrocarbyl having from 1-20 carbon atoms, wherein each may independently include at least one substituent selected from halo, oxo, hydroxyl, alkoxy, aryloxy, amino, amido or a detectable label; Y is O, S or Se; W is O, S, S(O), S(O) 2 , Se, C(O), C(S), C(O)NH, NH or NR 9 ; and Q is a modification group comprising one or more thermally cleavable groups.