Patent ID: 8343722

Claim:
A method based on fluorescence long (FL-DNA) analysis for determining the presence of colorectal tumors in a human subject, which comprises: a) extracting DNA from human stool samples; b) amplifying by PCR: i) p53 gene fragments corresponding to exons 5-8 using the following pairs of primers labeled with fluorescent markers: (SEQ ID NO: 1) 1) ctcttcctgcagtactcccctgc and (SEQ ID NO: 2) gccccagctgctcaccatcgcta, (SEQ ID NO: 3) 2) gattgctcttaggtctggcccctc and (SEQ ID NO: 4) ggccactgacaaccacccttaacc, (SEQ ID NO: 5) 3) gcgttgtctcctaggttggctctg and (SEQ ID NO: 6) caagtggctcctgacctggagtc, (SEQ ID NO: 7) 4) acctgatttccttactgcctctggc and (SEQ ID NO: 8) gtcctgcttgcttacctcgcttagt, and ii) APC gene fragments using the following pairs of primers labeled with fluorescent markers: (SEQ ID NO: 9) 1) aactaccatccagcaacaga and (SEQ ID NO: 10) taatttggcataaggcatag, (SEQ ID NO: 11) 2) cagttgaactctggaaggca and (SEQ ID NO: 12) tgacacaaagactggcttac, (SEQ ID NO: 13) 3) gatgtaatcagacgacacag and (SEQ ID NO: 14) ggcaatcgaacgactctcaa, (SEQ ID NO: 15) 4) cagtgatcttccagatagcc and (SEQ ID NO: 16) aaatggctcatcgaggctca; c) quantifying the amplified fragments and identifying the amplified fragments as amplicons; d) calculating the total amount of different amplicons; and e) comparing the total amount of amplicons with a reference value determined on the basis of a case series comprising healthy subjects and patients affected by colorectal tumors, wherein a total amount of amplicons higher than the reference value is indicative of the presence of colorectal tumors in said human subject.