Patent ID: 8304527

Claim:
A kit to execute a method of simultaneously screening for genetic mutations in different genes in a multitude of samples, the kit comprising: an antisense deoxyribonucleic acid (DNA) probe, wherein the antisense DNA probe: includes an extra deoxyribonucleotide at the 5′ end; and is configured to be mixed with a strand of a ribonucleic acid (RNA) to be tested to form a heteroduplex molecule within a sample; wherein the sample forms: a first population of fully hybridized wild-type heteroduplexes; a second population of mutant heteroduplexes having a single unhybridized ribonucleotide; and a third population of homologous heteroduplexes having at least one stretch of two or more unhybridized ribonucleotides; and a blocking adapter, wherein the blocking adapter: includes a dually phosphorylated sense strand; and is configured to protect a 3′ end of the RNA strand.