Patent ID: 7935541

Claim:
An immunochromatography method comprising, developing an analyte and a labeling substance which is modified with a first binding substance against the analyte in a mixed state on a porous carrier, wherein the porous carrier comprises a detection site and a non-detection site, capturing the analyte and the labeling substance at the detection site on the porous carrier having a second binding substance against the analyte or a substance capable of binding to the first binding substance against the analyte, wherein the labeling substance is non-specifically adsorbed on the porous carrier at both the detection site and the non-detection site, and performing amplification by adding an amplification solution containing a compound comprising silver and a reducing agent for silver ions, so as to detect the analyte, wherein a fragmented antibody is used as at least one of the binding substances, a density of the non-specifically adsorbed labeling substance is 10 6 /mm 2 or less; and a ratio of the number of the non-specifically adsorbed labeling substance in the detection site and that in the non-detection site of the porous carrier is between 0.4 to 2.5 before amplification.