Patent ID: 7618653

Claim:
A method for preparing a biological nerve guide, comprising: providing a natural animal tissue membrane; crosslinking and fixing the membrane; applying an active reagent to block specific active groups in the proteins of the membrane; applying a guanidine compound to replace the specific hydrogen bonding in the spiral chains of the protein molecules in the membrane and alter its specific conformation; tanning the membrane; coupling a polypeptide or glycosaminoglycan to an inner surface of the membrane to form an active layer; cutting the membrane; positioning the cut membrane onto a rod-shaped mold so that the cut membrane assumes a cylindrical configuration having an outer surface; and attaching a spiral support to the outer surface of the cut membrane; wherein the polypeptide is the polypeptide obtained from the condensation of 16 lysines (K16), glycine (G), arginine (R), asparagic acid (D), serine (S), proline (P) and cysteine (C), and the glycosaminoglycan is hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparin, acetylheparin sulfate or keratan sulfate.