Patent ID: 7456020

Claim:
A method for causing splitting, loss or deletion of a chromosome in a yeast comprising: preparing a linear chromosome splitting vector (1) in an order consisting essentially of a target sequence (a), a marker gene sequence and (C 4 A 2 ) n sequence (x); preparing a linear chromosome splitting vector (2) in an order consisting essentially of a target sequence (b), a centromere sequence of a yeast chromosome and (C 4 A 2 ) n sequence (y); simultaneously introducing the chromosome splitting vectors (1) and (2) into a yeast; and selecting yeast on the basis of marker gene expression, wherein the chromosome splitting vectors (1) and (2) are obtained directly by a PCR reaction, wherein at least one primer used in each PCR reaction includes the (C 4 A 2 ) n sequence, and wherein n is each independently an integer of 6 to 10.