Patent ID: 8211684

Claim:
A method for preparing a solution of stabilized thermolysin (EC 3.4.24.27), the method comprising: (a) the first step of mixing a solid preparation comprising thermolysin with an aqueous solvent and making a first solution, wherein the first solution comprises a first buffer salt capable of maintaining a pH in the range of 4.5 to 9, one or more second salts, and thermolysin, wherein in the first solution the aggregate concentration of the first and second salts is in the range of about 0.1 mM to 500 mM, (b) the second step of adding to the first solution a measured amount of a third salt, wherein the third salt is selected from the group consisting of NaCl, NaBr, NaNO 3 , Nal, KCl, LiCl, MgCl 2 , CaCl 2 , and a mixture thereof, and (c) dissolving the third salt in the first solution, thereby obtaining a second solution in which the dissolved thermolysin is in a stabilized form.