Patent ID: 7691635

Claim:
A temperature-stable labeling reagent of formula (0): in which: R 1 represents H or an alkyl, aryl or substituted aryl group, R 2 represents a detectable label or at least two detectable labels interlinked by at least one multimeric structure, the detectable label being at least one label capable of directly or indirectly generating a detectable signal, and the detectable label being selected from the group consisting of an enzyme, a chromophore, a group with an electron density detectable by electron microscopy, a group with an electron density detectable by its electrical property, a radioactive molecule, and indirect systems, L is a linker arm comprising a linear chain of at least two covalent bonds and n is an integer equal to 0 or 1, R 3 and R 4 represent, independently of one another: H, NO 2 , Cl, Br, F, I, R 2 -(L) n -Y—X—, OR, SR, NR 2 , R, NHCOR, CONHR, COOR, —CO—NH—(CH 2 ) 3 —(O—CH 2 —CH 2 ) 3 —CH 2 —NH—R 2 , or —CO—NH—(CH 2 ) 3 —(O—CH 2 —CH 2 ) 4 —CH 2 —NH—R 2 with R=alkyl or aryl, A is a linker arm comprising at least one covalent double bond enabling the conjugation of the diazo function with the aromatic ring and u is an integer between 0 and 2, —Y—X— represents —CONH—, —NHCO—, —CH 2 O—, or —CH 2 S—, —Z— represents —NH—, —NHCO—, or —CONH—, m is an integer between 2 and 10, and p is an integer between 1 and 10.