Patent ID: 7833741

Claim:
A method of making a heterologous polypeptide comprising: (i) propagating a modified T7 phage in an E. coli cell, wherein the modified T7 phage comprises, in a 5′ to 3′ orientation, a promoter nucleic acid sequence, which is operably linked to a nucleic acid sequence encoding a surface protein of the modified T7 phage, which is operably linked to a nucleic acid sequence comprising a termination codon, which is operably linked to a nucleic acid sequence encoding the heterologous polypeptide; wherein the E. coli cell comprises a suppressor construct comprising an inducible promoter nucleic acid sequence, which is operably linked in a 5′ to 3′ orientation to a nucleic acid sequence encoding a suppressor tRNA that binds to the termination codon of the modified T7 phage; wherein expression of the suppressor tRNA is not induced; and (ii) inducing the inducible promoter of the suppressor construct; whereby the suppressor tRNA is expressed in the E. coli cell so that the heterologous polypeptide is expressed and displayed on the surface of the modified T7 phage.