Patent ID: 7612195

Claim:
A retroviral vector for carrying a target gene specific insert into a cell in order to modify the expression of a target gene having a sense strand and an antisense strand, comprising: (a) a U6 promoter having a sequence of: (SEQ ID NO:7) ttcccatgattccttcatatttgcatatacgatacaaggctgttagagag ataattagaattaatttgactgtaaacacaaagatattagtacaaaatac gtgacgtagaaagtaataatttcttgggtagtttgcagtttttaaaatta tgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcga tttcttgcctttatatatcttgtggaaaggacgaaacaccg; and (b) a polylinker region comprising a nucleotide sequence of gatcc gctgggactcctttgcatg ttcaagaga catgcaaaggagtcccagc ttttt ggaa a (SEQ ID NO:4) (c) a target gene specific insert comprising double stranded RNA, wherein said double stranded RNA comprises a sense portion that is complementary to a portion of the antisense strand of the target gene, and an antisense portion that is complementary to the sense portion, so that the sense portion and antisense portion anneal, and the double stranded RNA folds back upon itself.