Patent ID: 8071750

Claim:
A composition comprising an invasive cleavage detection assay, wherein said invasive cleavage detection assay comprises two or more probe sets, wherein each probe set comprises a first and second oligonucleotide configured to form an invasive cleavage structure to detect at least a single nucleotide polymorphism in a position of a 5â€² UTR sequence of HCV and wherein said first oligonucleotide comprises a 5â€² portion and a 3â€² portion, wherein said 3â€² portion is configured to hybridize to said 5â€² UTR sequence of HCV, and wherein said 5â€² portion is configured to not hybridize to said 5â€² UTR sequence of HCV, wherein said first oligonucleotide is a sequence selected from the group consisting of SEQ ID NOs: 2, 33, 34, 36, and 63, wherein nucleotide positions in said 5â€² UTR are numbered in accordance with the consensus sequences SEQ ID NOS:64, 65, 66, 67, 68 and 69, and wherein said position of said at least a single nucleotide polymorphism is selected from the group consisting of: âˆ’245, âˆ’167, âˆ’163, âˆ’155, âˆ’144, âˆ’118, and âˆ’80, wherein position âˆ’245 is at nucleotide 30 in SEQ ID NOS:64-69; wherein position âˆ’167 is at nucleotide 108 of SEQ ID NOS:64-69; wherein position âˆ’163 is at nucleotide 112 of SEQ ID NOS:64-69; wherein position âˆ’155 is at nucleotide 120 of SEQ ID NOS:64-69; wherein position âˆ’144 is at nucleotide 131 in SEQ ID NO:69 and is not present in SEQ ID NOS:64-68; wherein position âˆ’118 is at nucleotide 157 in SEQ ID NOS:64-68 and nucleotide 160 in SEQ ID NO:69; and wherein position âˆ’80 is at nucleotide 195 in SEQ ID NOS:64-68 and nucleotide 198 in SEQ ID NO:69.