Patent ID: 7906334

Claim:
A method for reproducing coniferous somatic embryos by somatic embryogenesis comprising growing on a nutrient medium an immature embryogenic culture derived from an explant, the nutrient medium selected from the group consisting of induction medium, maintenance medium and prematuration medium, and the nutrient medium comprising lactose, wherein the coniferous somatic embryos are Pinus taeda somatic embryos or hybrids thereof, and wherein when the nutrient medium is the induction medium, growing the immature embryonic culture comprises inducing the explant to form an embryogenic tissue, wherein when the nutrient medium is the maintenance medium, growing the immature embryonic culture comprises, growing and maintaining the embryogenic culture, and wherein when the nutrient medium is the prematuration medium, growing the immature embryonic culture further comprises transferring the embryogenic culture from the prematuration medium to maturation medium for subsequent development of mature embryos capable of germination.