Patent ID: 7723080

Claim:
A method of preparing a sialyl Lewis x determinant, the method comprising contacting a recombinant murine α(1,3)fucosyltransferase (Fuc-TVII) enzyme catalytic domain with a GDP-fucose donor substrate and a sialyl-N-acetyl-lactosamine acceptor substrate in an enzyme bioreactor under conditions that allow the addition of an α1,3 linked fucose to the sialyl-N-acetyl-lactosamine acceptor substrate, wherein the recombinant murine Fuc-TVII enzyme comprises a catalytic domain is encoded by a nucleic acid sequence segment that is identical to a polynucleotide that is amplified using murine cDNA shown in SEQ ID NO:1 as a template by a 5′ primer as shown in SEQ ID NO:3 and a 3′ primer as shown in SEQ ID NO:4.