Patent ID: 8741612

Claim:
A non-naturally occurring microbial organism comprising an isoprene biosynthetic pathway for the conversion of dimethylallyl diphosphate (DMAPP) to isoprene, wherein the isoprene biosynthetic pathway comprises the conversion of DMAPP to 2-methyl-3-buten-2-ol followed by the conversion of 2-methyl-3-buten-2-ol to isoprene and wherein the isoprene biosynthetic pathway comprises an exogenous nucleic acid encoding at least one 2-methyl-3-buten-2-ol synthase capable of converting DMAPP to 2-methyl-3-buten-2-ol and an exogenous nucleic acid encoding at least one 2-methyl-3-buten-2-ol dehydratase capable of converting 2-methyl-3-buten-2-ol to isoprene and wherein the isoprene biosynthetic pathway is expressed at a sufficient level to produce isoprene.