Patent ID: 7811753

Claim:
A method for repairing and amplifying degraded DNA fragments in vitro comprising: I) contacting said degraded DNA fragments with a reaction mixture comprising: a) a plurality of substantially purified enzymes, said enzymes having endonuclease activity, DNA N-glycosylase activity, AP lyase activity, polymerase activity, 3′-diesterase activity, polynucleotide kinase activity, and DNA ligase activity, and b) a reaction buffer, wherein said reaction buffer further comprises ATP, wherein said contacting is under conditions such that said degraded DNA fragments are repaired, 5′ end phosphorylated, and ligated together to generate repaired concatamerized DNA; II) purifying said repaired concatamerized DNA from said plurality of substantially purified enzymes to generate purified repaired concatamerized DNA; and III) amplifying said purified repaired concatamerized DNA using a whole genome amplification method, wherein said whole genome amplification method employs random primers.