Patent ID: 8158608

Claim:
A fraction from Zingiber officinale comprising pectic polysaccharides constituted by 1 to 2% of rhamnose, 21 to 24% of arabinose, 6 to 8% of xylose, 2 to 3% of mannose, 3 to 5% of galactose and 50 to 58% of glucose, optionally along with an antioxidant comprising a phenolic acid selected from the group consisting of 42 to 46% of gallic acid or 42-46% of tannic acid, 0.6 to 0.8% of gentisic acid, 0.5 to 0.6% of protacatechuic acid, 0.1 to 0.2% of vanillic acid, 0.05 to 0.1% of caffeic acid, 0.1 to 0.2% of syringic acid and 48 to 50% of cinnamic acid, wherein the fraction exhibits proton Potassium ATPase inhibition activity, wherein said fraction is obtained by a process comprising: a) pulverizing ginger rhizome to a particle size of 20 mesh to form a powder; b) refluxing the powder obtained from step a) with chloroform and petroleum ether in a ratio of 1:1 v/v at a ratio of 1:5 w/v to form a defatted powder followed by air drying the defatted powder; c) adding 70% ethanol in the air dried defatted powder obtained from step b) in the ratio of 1:3 w/v and vortexing for 60 minutes for 3 times to remove free phenolics and soluble sugars; d) centrifuging a mixture obtained from step c) at a speed of 5000 to 6000 g for a period of 10 to 15 minutes to form a residue and supernatant; e) lyophilizing the supernatant obtained from step d) to get a dry lyophilized powder (GRAOX) having proton potassium ATPase inhibition (PPI) and antioxidant activity (AOX); f) air drying the residue obtained from step d); g) treating the air dried residue obtained from step f) with 50 U/100 g of protease in 100 mM phosphate buffer pH 7.4 for 8 to 10 hours at 37° C. and centrifuged at 8000 to 10,000 g for 10 minutes to remove the protein contaminant and to obtain a deproteinated residue; h) boiling the deproteinated residue obtained from step g) in 50 mM acetate buffer pH 4.6 and treating with 0.25 μ/g thermoamylase for 30 to 40 minutes and centrifuging at 8000 to 10,000 g for 15 minutes; i) cooling a residue obtained from step h) with 7 μ/g glucoamylase in 50 mM acetate buffer pH 4 for 1 to 2 hours for complete removal of starchy polysaccharide followed by centrifugation at 8000 to 10,000 g for 15 minutes; j) boiling a residue obtained from step i) with 0.05% ammonium oxalate solution for 60 to 180 minutes at 70° C. to isolate a pectic polysaccharide (GRPP) rich fraction; k) concentrating the GRPP rich fraction obtained from step j) by flash evaporation to form a concentrate; l) dialyzing the concentrate obtained from step k) against distilled water in a dialysis membrane of 10,000 cut off to remove ammonium oxalate salts; m) lyophilizing a dialyzed sample obtained from step 1) to get a desired pectic polysaccharide with a yield of approximately 6% designated as GRPP.