Patent ID: 8143008

Claim:
A method for amplifying nucleic acid molecules comprising: a) providing a plurality of immobilized primers and immobilized first single stranded nucleic acid molecules; b) allowing the immobilized first single stranded nucleic acid molecules to anneal to primers in the plurality of immobilized primers; c) extending the primers that are annealed to the immobilized first single stranded nucleic acid molecules using the immobilized first single stranded nucleic acid molecules as template to provide second immobilized nucleic acid molecules; d) separating the template and second immobilized nucleic acid molecules using a chemical denaturant; e) allowing the template and second immobilized nucleic acid molecules to anneal to primers in the plurality of immobilized primers; f) extending the primers that are annealed to the template and second immobilized nucleic acid molecules in suitable conditions; and g) repeating steps d)-f) to generate multiple copies of the nucleic acid molecules.