Patent ID: 7968286

Claim:
A method for determining a titer of neutralizing antibodies directed against adenoviruses in a human- or animal-derived blood sample, the method comprising: obtaining a serum sample of the human or animal; culturing host cells which can be infected with a recombinant adenovirus; admixing the serum sample, the host cell culture and the recombinant adenovirus, wherein the recombinant adenovirus comprises a transgene encoding luciferase; incubating the admixture for about 24 hours; determining the activity of luciferase in the admixture; and comparing the activity of luciferase in the admixture to a serum-free control, thereby determining the titer of neutralizing antibodies in the blood sample; wherein the titer of neutralizing antibodies is determinable with 1000 host cells.