Patent ID: 8519227

Claim:
A method for obtaining at least one transformed rice plant having roots and leaves in 50 days or less comprising: pre-culturing intact rice seeds on a medium containing an auxin for 4 to 5 days to obtain precultured rice seeds; wherein the pre-culturing step is completed before the seeds dedifferentiate, wherein the pre-cultured seeds are seeds or germinated seeds; infecting the precultured intact rice seeds with Agrobacterium containing a recombinant DNA of interest and incubating the seeds for 2 to 5 days in the dark to obtain infected rice seeds; selecting for seeds containing the DNA of interest by placing, for 14 days, the infected rice seeds on a medium containing a substance which selects for seeds containing the DNA of interest; and regenerating the selected seeds to obtain at least one transformed rice plant having roots and leaves.