Patent ID: 8153412

Claim:
An isolated variant of a parent Bacillus sp. TS-23 alpha-amylase, wherein the variant comprises an amino acid sequence with at least 90% amino acid sequence identity to SEQ ID NO: 2, wherein the variant has amylase activity, and wherein said variant comprises a truncation of the C-terminus, the modification S243Q, A, E, or D, and optionally any one or more of modifications (a) to (i), wherein each amino acid position corresponds to a position in SEQ ID NO: 1: (a) R180 deletion or S181 deletion or R180 and S181 deletion; (b) M201L; (c) Q87 to E or R; (d) N225 to E or R; (e) N272 to E or R; (f) N282 to E or R; (g) T182 deletion; (h) G183 deletion; or (i) Q98R, M201L, S243Q, R309A, Q320R, Q359E, and K444E.