Patent ID: 7754697

Claim:
A double-stranded synthetic DNA gene, comprising multiple copies of a structural gene region, wherein the structural gene region comprises a nucleotide sequence which consists of greater than 20 consecutive nucleotides and which is identical to a nucleotide sequence of a target gene in a eukaryotic cell, wherein one of the copies is placed in the sense orientation and another of the copies is placed in the antisense orientation operably under the control of a single promoter sequence which is operable in the cell, wherein the copy of the structural gene region placed in the sense orientation and the copy of the structural gene region placed in the antisense orientation are arranged so as to form an interrupted palindrome sequence which is operably under the control of the single promoter sequence, and wherein the structural gene region placed in the sense orientation and the structural gene region placed in the antisense orientation are separated by a sequence of nucleotides that is 50-100 nucleotides in length or 100-500 nucleotides in length.