Patent ID: 7608258

Claim:
A method of vascularizing an area of tissue injury in a mammal comprising: drawing autologous blood from the mammal; adding an anticoagulant to the autologous blood; fractioning the autologous blood in a disposable separating tube using a centrifugation device to isolate a platelet-rich plasma composition; titrating the platelet-rich plasma composition to obtain a pH of about 6.5 to 8.0 creating a buffered platelet-rich plasma; and administering the buffered platelet-rich plasma to the area of tissue injury of the mammal, whereby platelet growth factors are released from the buffered platelet-rich plasma, wherein no exogenous activator is administered to the mammal or added to the platelet-rich plasma composition prior to its introduction to tissue with a compromised blood supply, and wherein the tissue with has a compromised blood supply comprises a tendinosis.