Patent ID: 7790429

Claim:
A process for the preparation of short-chain alkyl esters of fatty acids in solvent-free system comprising: providing a fatty acid source, stepwise adding a short-chain free alcohol or any other alcohol donor, to said fatty acid source in the presence of a lipase preparation and allowing the reaction to proceed under suitable conditions, until said fatty acid source triglycerides are converted to fatty acid alkyl esters, wherein said lipase preparation comprises at least two lipases, said lipases being separately or jointly immobilized on a suitable support and wherein at least one of said lipases has increased affinity for partial glycerides is derived from any one of Candida antarctica B, Alcaligenes sp. and Penicillium camembertii and at least one of said lipases is sn-1,3 positional specific and is derived from any one of Thermomyces lanuginose, Rhizomucor miehei, Mucor javanicus, Pseudomonas sp., Penicillium roqueforti, Aspergillus niger, Acromobacter sp. and Burkholderia sp.