Patent ID: 7794966

Claim:
A method of reducing an influence of a non-analyte glycated amine during a determination of an amount of a glycated protein as an analyte, comprising: (a) pretreating a sample by adding a first fructosyl amino acid oxidase (FAOD) to the sample so that the first FAOD acts on a non-analyte glycated amine that is present in the sample and different from a glycated protein as an analyte, thereby reducing an influence of the non-analyte glycated amine on a determination of an amount of the glycated protein as the analyte; (b) adding a protease to the sample, thereby degrading the glycated protein as the analyte contained in the sample with the protease; (c) after step (b), causing a redox reaction to occur without performing either of the following: (1) adding an additional amount of the first FAOD, and (2) adding a second FAOD that is different from the first FAOD, or a separate FAOD so that in the redox reaction, the first FAOD added in the pretreatment acts on the degradation product of the glycated protein; measuring the redox reaction; and determining the amount of the glycated protein based on a result of the measurement of the redox reaction.