Patent ID: 7920908

Claim:
A method of imaging a highly light scattering medium comprising: a) illuminating the medium with multiple wavelengths of filtered, processed light; b) calibrating an image by iteratively integrating parameters for each reconstruction point, wherein calibrating comprises; i) arbitrarily assigning, at each pixel within an image, parameter values to each known component comprising the medium, wherein parameter values are selected from a plurality of values; ii) executing an algorithm to converge a calibration function comprising calculating calibration coefficients by fixing one or more parameters at one value and solving for other one or more parameters, thereby obtaining average calibration loop pixel parameter values; iii) estimating convergence between the fixed one or more parameter values and the other one or more parameter values; iv) adjusting calibration by imposing one or more spatial characteristics for the other one or more parameter values to smooth said other one or more parameter values to form fixed one or more parameter values; and v) repeating steps (ii), (iii), and (iv) until one or more predetermined conditions are satisfied; c) reconstructing local concentration variations of absorbing/light-scattering components comprising the medium, comprising comparing collected wavelengths; and d) converting reconstructed data to form output images.