Patent ID: 7544776

Claim:
A functional engineered fluorescent protein whose amino acid sequence is at least 85% identical to the amino acid sequence of Aequorea green fluorescent protein set forth in SEQ ID NO:2 and which differs at least from SEQ ID NO:2 by i) comprising the substitutions T203Y; S65G; S72A; V68L; and either of H148Q or H148G; and ii) further comprising at least one substitution selected from the group consisting of R96K; V150S, T, Q, NA, C, M, or G; I152L, V, F, S, T, Q, N, A, C, M, or G; F165L; H181F, W, K or R; Q183R, N, S, or C; and L201S, T, Q, N, V, I, A, C, M, or G; wherein said functional engineered fluorescent protein has a different fluorescent property and altered anion binding affinity as compared to the Aequorea green fluorescent protein having the amino acid sequence set forth in of SEQ ID NO:2, and wherein said functional engineered fluorescent protein has fluorescent emission in the visible spectrum.