Patent ID: 7923430

Claim:
A composition of recombinant human glycoproteins comprising the isolated recombinant human glycoproteins wherein in excess of 30 mole % of the oligosaccharide structures on the isolated recombinant human glycoproteins in the composition have a structure consisting of GlcNAcMan 5 GlcNAc 2 , and wherein the recombinant human glycoproteins are suitable substrates to be trimmed in vivo by the action of a mannosidase to form complex N-glycans, and wherein the isolated recombinant human glycoproteins are prepared from a host cell which is yeast or filamentous fungus genetically engineered to produce said 30 mole % of the oligosaccharide structures having a structure consisting of GlcNAcMan5GlcNAc2.