Patent ID: 8298334

Claim:
A method of crystallizing T1 lipase from Geobacillus sp. strain T1, wherein the method comprises the following steps: a. Providing purified mature T1 lipase from Geobacillus sp. strain T1 to be crystallized, wherein said purified mature T1 lipase is prepared by expressing a T1 lipase gene in plasmid pGEX/T1S as a Glutathione-S-Transferase (GST) fusion protein, cleaving the expressed GST fusion protein by thrombin, and purifying said mature T1 lipase from GST and thrombin, wherein said purified mature T1 lipase has an estimated molecular weight of 43,000 Da; b. Preparing a crystallization mix comprising a buffer comprising 1 to 2M NaCl, 0.1M NaH 2 PO 4 , 0.1M KH 2 PO 4 , and 1.0M MES pH 6.5, and 0.5 to 4.5 mg/mL of purified mature T1 lipase from step (a); and c. Obtaining T1 lipase crystals from the crystallization mix of step (b) by a hanging-drop methodology, wherein crystals of the lipase are formed between about 1 hour and 1 day.