Patent ID: 8497117

Claim:
A method for producing a cell culture substrate, comprising the steps of: preparing a patterning substrate comprising a base material and a cell culture patterning layer formed on the base material and containing a cell adhesion material, wherein the cell adhesion material has adhesive properties to cells and is decomposed or denatured upon energy irradiation of a photocatalyst; preparing a photocatalyst-containing substrate comprising a base body and a photocatalyst-containing layer formed on the base body and containing a photocatalyst; arranging the cell culture patterning layer of the patterning substrate and the photocatalyst-containing layer of the photocatalyst-containing substrate to face each other, irradiating an energy from a predetermined direction onto the cell culture patterning layer, and thereby forming a cell adhesion-inhibiting portion and a cell adhesion portion in a pattern, wherein the cell adhesion-inhibiting portion is the cell adhesion material decomposed or denatured by action of the photocatalyst and the cell adhesion portion is other than the cell adhesion-inhibiting portion; and applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method whereby the cell-containing liquid is selectively applied onto only the cell adhesion portion.