Patent ID: 8158407

Claim:
A method of coating a hybrid vascular graft comprising a hemodialysis access graft or a femoral artery graft with a confluent monolayer of endothelial cells, said method comprising: (a) immobilizing a plurality of multipotent stem cells on at least one surface of a specimen comprising a hemodialysis access vascular graft or a femoral artery graft; and (b) placing said specimen in a system under conditions effective to promote said stem cells to form confluent monolayer on said surface of said specimen, wherein said system comprises: a contoller having at least one input for receiving information and feedback information and an output for outputting control signals; and a pressure/flow loop subsystem coupled to said controller, comprising: a specimen holder, an eternal flow loop system coupled to said specimen holder, and an output for outputting said feedback information, wherein said pressure/flow loop subsystem receives said control signals and is capable of independent controlling pressure and flow rate at said specimen in accordance with said control signals, when said specimen holder contains said specimen; and (c) placing said specimen in an environment that promotes said stem cells to differentiate into endothelial cells.