Patent ID: 8753688

Claim:
A bear bile macromolecular extract with anti-hepatitis C virus function, characterized in that the extract is prepared by the following method: add water to dissolve bear bile powder to obtain aqueous solution of bear bile in concentration of 10˜100 mg/ml, centrifuge the solution by a molecular sieve filter membrane with molecular weight cut-off of 100,000 at the centrifugal force of 4000×g for 10˜60 minutes, or centrifuge fresh bear bile by a molecular sieve filter membrane with molecular weight cut-off of 100,000 at the centrifugal force of 4000×g for 1˜2 hours; filter to obtain a sediment, dissolve the sediment in water to obtain a solution, add the solution to a pretreated cross-linked dextran column having a dry particle size of 40 μm-120 μm and a globular protein fractionation range of 4×10 3 -1.5×10 5 , separate the solution by using water or buffer as elution solvent, collect the eluent, including the void volume eluent, with absorption at 280 nm, freeze-dry the collected eluent to obtain the bear bile macromolecular extract.