Patent ID: 6852849

Claim:
An isolated polynucleotide encoding at least a first monomer of a non-oligomerizing tandem fluorescent protein, wherein the non-oligomerizing tandem fluorescent protein comprises a first monomer of an Aequorea green fluorescent protein (GFP) or a fluorescent protein related to an Aequorea GFP operatively linked to at least a second monomer of an Aequorea GFP or a fluorescent protein related to an Aequorea GFP, and wherein said first monomer of the non-oligomerizing tandem fluorescent protein comprises a mutation of an amino acid residue corresponding to A206K, L221K, F223R, or a combination thereof of SEQ ID NO: 6 or SEQ ID NO: 10, wherein the numbering of the amino acid residues conforms to that in native Aequorea GFP (SEQ ID NO: 2), and wherein the propensity of the tandem fluorescent protein to form intermolecular oligomers is reduced or inhibited as compared to a monomer of an Aequorea GFP or a fluorescent protein related to an Aequorea GEP.