Patent ID: 7238526

Claim:
A method for producing recombinant AAV, said method comprising the steps of: infecting a helper-containing host cell useful for production of recombinant adeno-associated virus (AAV), wherein said cell has stably integrated in its chromosome at least two copies of an AAV rep gene and AAV cap gene under the control of regulatory sequences comprising AAV P5 which is located in the native AAV position upstream of the AAV rep gene with a recombinant hybrid virus, the recombinant hybrid virus comprising: (1) a selected transgene operatively linked to regulatory sequences controlling the transgene's expression, (2) AAV sequences comprising the 5′ and 3′ ITRs of an AAV, wherein the 5′ ITR flanks one side of the transgene and regulatory sequences of (1), and the 3′ ITR flanks the other side, and (3) an adenovirus cis-element selected from the group consisting of cis-elements required for replication of adenovirus genomes or genes and cis-elements required for encapsidation of adenovirus virions, said cis-elements flanking each AAV sequence of (2); whereby recombinant AAV is produced by the cell.