Patent ID: 8889393

Claim:
A method of serial recovery of two different nucleic acid species from a biological sample, comprising: in a first binding step, homogenizing and enzymatically digesting the biological sample in a combined lysis and nucleic acid binding buffer, thereby generating a lysate comprising a first nucleic acid species and a second nucleic acid species, followed by selectively binding the first nucleic acid species to a first solid phase by contacting the lysate in the combined lysis and nucleic acid binding buffer with the first solid phase that selectively binds the first nucleic acid species; separating the first solid phase with the bound first nucleic acid species from an unbound portion of the lysate; in a second binding step, binding the second nucleic acid species to a second solid phase, different from the first solid phase, by contacting an unbound portion of the lysate in the combined lysis and nucleic acid binding buffer with the second solid phase that binds the first and second nucleic acid species; enzymatically digesting the first nucleic acid species bound to the second solid phase; and isolating the second nucleic acid species from the second solid phase.