Patent ID: 8765393

Claim:
A method of selective removal of endotoxins from an aqueous system comprising one or more antibiotics and/or proteins wherein said method comprises the use of an affinity ligand-matrix conjugate of the structure Z-Spacer-Ligand wherein the ligand is tris(2-aminoethyl)amine; wherein the ligand is attached to a support matrix Z through the spacer, wherein said endotoxins are lipopolysaccharides (LPS) from gram-negative bacteria, and wherein the method comprises: contacting the affinity ligand-matrix conjugate with the aqueous system under conditions such that endotoxins in the aqueous system bind directly to the ligand, and recovering the aqueous system that has been contacted with the affinity ligand matrix conjugate and from which endotoxins have been removed; wherein essentially all of the protein and/or antibiotic remains in the aqueous system.