Patent ID: 7741027

Claim:
A mixture of nucleic acid oligomers for specifically amplifying a plurality of nucleotide sequences in distinct regions of the HIV-1 genome in pol and gag gene sequences, comprising: oligomers specific for a region in the pol sequence, referred to as a RT1 sequence, that include an oligonucleotide in a size range of 18 to 23 nucleotides that contains the sequence consisting of SEQ ID NO:14 or an oligonucleotide that contains a 5′ promoter sequence covalently attached to a sequence in a size range of 18 to 23 nucleotides that contains the sequence consisting of SEQ ID NO:14, and an oligonucleotide in a size range of 19 to 20 nucleotides that contains the sequence consisting of SEQ ID NO:64, oligomers specific for a region in the pol sequence, referred to as a RT4 sequence, that include an oligonucleotide consisting of SEQ ID NO:16 or an oligonucleotide that contains a 5′ promoter sequence covalently attached to the sequence consisting of SEQ ID NO:16, and an oligonucleotide consisting of SEQ ID NO:22, and oligomers specific for a region in the gag sequence, referred to as a Gag2 sequence, that include an oligonucleotide consisting of SEQ ID NO:12 or an oligonucleotide that contains a 5′ promoter sequence covalently attached to the sequence consisting of SEQ ID NO:12, and an oligonucleotide consisting of SEQ ID NO:18.