Patent ID: 7552698

Claim:
A triploid breeding method for obtaining triploids of cold-warm water shrimp or tropical shrimp which comprises (a) concentrating fertilized eggs from the cold-warm water shrimp after spawning at a temperature of 15-16° C., and (a1) subjecting the fertilized eggs to heat shock treatment by exposing the fertilized eggs to seawater having a temperature of 30-31° C. for a duration of 8-10 minutes, wherein the fertilized eggs were concentrated by allowing the fertilized eggs to settle and then removing a layer of seawater above the fertilized eggs, to obtain meiosis I (MI) triploids, or (a2) collecting the concentrated fertilized eggs with a sieve cloth, and subjecting to treatment with a revulsant 0.5-0.6% (w/v) PC solution at 30-34 minutes after spawning for a duration of 15-18 minutes; or (b) concentrating fertilized eggs from the tropical shrimp after spawning at 27-28° C., and (b1) subjecting the fertilized eggs to cold shock treatment by exposing the fertilized eggs to seawater having a temperature of 5-10° C. for a duration of 15-20 minutes, or (b2) treating the fertilized eggs with a seawater solution comprising 200-400 μM 6-DMAP for a duration of 10-12 minutes.