Patent ID: 8415134

Claim:
A process for purifying a poxvirus composition from cell culture supernatant and cultured packaging cells infected with a wild type, an attenuated, and/or a recombinant poxvirus with no targeted infection specificity, comprising the steps of: a) recovering poxyiral particles from the cell culture supernatant and from cultured packaging cells disrupted by high speed homogenizer, to obtain a poxyiral particle mixture, b) clarifying said poxyiral particle mixture, thereby removing cellular debris, wherein said clarifying comprises depth filtration, to obtain a clarified poxyiral particle mixture, c) concentrating said clarified poxyiral particle mixture, wherein said concentrating comprises microfiltration, to obtain a concentrated poxyiral particle mixture, and d) diafiltrating said concentrated poxyiral mixture to obtain a composition of a wild type, an attenuated, and/or a recombinant poxvirus with no targeted infection specificity wherein said process is free from animal products and does not use nuclease, and wherein said poxvirus composition comprises intracellular mature virus (“IMV”) and extracellular enveloped virus (“EEV”) with more than 1% EEV.