Patent ID: 7097999

Claim:
A method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium containing glucose and an oligosaccharide, allowing said substance to accumulate in the medium, and collecting said target substance from the medium, wherein the microorganism is a mutant or recombinant strain of a microorganism in which maltose assimilation is controlled by an interaction between IIA Glc protein of glucose PTS and MalK, and wherein said interaction in the mutant or recombinant strain is reduced or eliminated because said MalK protein has a mutation selected from the group consisting of substituting a Thr residue for the Ala residue at the position corresponding to position 124 in E. coli MalK and substituting a Gln residue for the Leu residue at the position corresponding to position 327 in E. coli MalK; and/or said IIA Glc protein has a mutation selected from the group consisting of substituting a Ser residue for the Gly residue at the position corresponding to position 47 in E. coli IIA Glc protein and substituting a Thr residue for the Ala residue at the position corresponding to position 76 in E. coli IIA Glc protein; and wherein said strain can take up glucose and maltose.