Patent ID: 8148316

Claim:
A process to disinfect a hard surface or inanimate object using an enzymatically-produced peroxycarboxylic acid composition, said process comprising: (a) providing a set of reaction components, said components comprising: (1) a formulation comprising a mixture of: (I) at least one carboxylic acid ester selected from the group consisting of monoacetin, diacetin, triacetin, monopropionin, dipropionin, tripropionin, monobutyrin, dibutyrin, tributyrin, and mixtures thereof; (II) an enzyme powder comprising (i) at least one enzyme structurally classified as a CE-7 enzyme and having perhydrolysis activity and a signature motif comprising: (A) an RGQ motif at amino acid residues 118-120, (B) a GXSQG motif at amino acid residues 179-183, and (C) an HE motif at amino acid residues 298-299 when aligned to reference sequence SEQ ID NO:1 using CLUSTALW, (ii) at least one excipient, and (iii) optionally at least one surfactant; and (III) at least one buffer; wherein said at least one buffer enhances the stability of said at least one enzyme when present in said formulation as compared to the perhydrolase activity of said at least one enzyme when the at least one excipient is not present; and (2) a source of peroxygen in water; (b) combining said reaction components whereby a peroxycarboxylic acid product is produced; (c) optionally diluting said peroxycarboxylic acid product; and (d) contacting said hard surface or inanimate object with the peroxycarboxylic acid produced in step (b) or step (c) whereby said surface or said inanimate object is disinfected.