Patent ID: 7887690

Claim:
A DNA separation device for separating, by electrophoresis, a first sample DNA including a first base sequence, and a second sample DNA including a second base sequence that is partially different from the first base sequence, which are contained in a sample solution, wherein a positive electrode and a negative electrode are provided on end portions at both ends of a capillary type hermetically-sealed flow path, respectively, so that the electrodes are immersed in a buffer solution; a conjugate DNA solution which is obtained by adding, in a buffer solution, a conjugate DNA that is obtained by combining a marker DNA having a specific base sequence with a non-electrophoresis material which performs electrophoresis at a speed that is negligible low relative to the electrophoresis speeds of the first sample DNA and the second sample DNA, is filled in the hermetically-sealed flow path; a presample solution including a first DNA complex which is obtained by combining the first sample DNA with a first ligand DNA that includes a first probe having a base sequence complementary to the first base sequence, and a second probe having a base sequence complementary to the base sequence of the marker DNA, and a second DNA complex which is obtained by combining the second sample DNA with a second ligand DNA that includes a third probe having a base sequence complementary to the second base sequence, and a fourth probe having a base sequence that is at least one base different from the base sequence of the second probe, is injected into the hermetically-sealed flow path; and voltage is applied to the positive electrode and the negative electrode to make the first DNA complex and the second DNA complex perform electrophoresis in the conjugate DNA solution, thereby separating the DNA complexes.