Patent ID: 7329491

Claim:
A method for purification of a nucleic acid comprising the steps of: (a) adsorbing the nucleic acid onto a substrate by contacting the substrate with a composition comprising: (i) an aqueous buffer, (ii) one or more salts having a concentration of about 1 to 10 M, (iii) a water-miscible, non-acidic organic compound selected from the group consisting of acetylacetone, dimethylsulfoxide, methylethylketone, methylpropylketone, isobutylmethylketone, gamma-butyrolactone, gamma-valerolactone, propylene carbonate, and N-methyl-2-pyrrolidone, and (iv) the nucleic acid; (b) contacting the substrate from step (a) with a solution comprising one or more salts having a concentration less than the concentration in step (a), thereby desorbing the nucleic acid from the substrate; and (c) separating the solution containing the desorbed nucleic acid from the substrate, thereby purifying the nucleic acid.