Patent ID: 8524469

Claim:
A Gram staining method, consisting of a primary staining step and a counter-staining step, and the primary staining step and the counter-staining step further comprising the sub-steps of: adding 10˜30 μl primary stain obtained from a reagent kit on a clean slide; adding specimens, volume of which is equivalent to said primary stain and coating uniformly on said slide, wherein said specimens include Gram-negative bacteria and Gram-positive bacteria; adding 30˜60 μl counter-stain on said slide for incubating 10˜20 seconds immediately; rinsing said slide with water and then drying said slide; and examining said slide under a microscope, wherein results appear that color contrast is present on the center of the slide in between said Gram-negative bacteria performing light red color and said Gram-positive bacteria performing deep purple color; wherein said primary stain consists of 0.2 to 0.5 wt. % crystal violet, and 4 to 6 wt. % sodium chloride solvent, and said counter-stain consists of 0.2 to 0.5 wt. % basic fuchsin, 0.1 to 0.3 wt. % potassium iodide, 0.05 to 0.15 wt. % iodine and 90 to 96 wt. % ethyl alcohol.