Patent ID: 8574922

Claim:
A portable device for targeted cell separation and enumeration, comprising: a lid, wherein the center of said lid contains a hollowed one-way turning button with at least two stops, the edge of said lid is configured with at least two cuts, and the body of said lid comprises a single opening, wherein a strip membrane is supported on said opening through an appropriate flow-through structure; wherein said single opening and said strip membrane form a single sample loading area that is permeable through said strip membrane; and wherein said strip membrane is configured by said targeted cell's pore size and collects said targeted cells as retentate; a base, wherein the center of said base contains a spring projection, the edge of said base is configured with at least two protuberances, wherein said protuberances fit to said cuts, and the body of said base contains at least two independent rooms, wherein each of said independent rooms is sized at least such that said strip membrane can reside entirely inside each said independent room; and wherein, when said cuts and said protuberances are aligned, the said independent room in which said strip membrane resides entirely and said single sample loading area together form said reaction well that is isolated from other said independent rooms; said lid and said base are configured that said strip membrane can reside inside any of said independent rooms when said cuts and said protuberances are aligned; pressing said spring projection separates said lid and said base; and turning once of said button rotates said lid and carries said single sample loading area with said lid to next said independent room such that said single sample loading area and next said independent room form next said reaction well; whereby said portable device allows separation and enumeration to be performed in single said portable device by sequentially rotating said single sample loading area to different said independent rooms to form different said reaction wells.