Patent ID: 8450066

Claim:
A method for determining whether a gene product has an activity of interest comprising: (a) treating a well of a mufti-well plate with a transfection reagent comprising a cationic lipid preparation, wherein said well includes a coating comprising a polycationic polymer; (b) adding to said well (i) a first cell preparation, (ii) a first vector selected from a library of vectors, at least two members of said library comprising genes which encode different test proteins, and (iii) a second vector comprising a gene which encodes a reporter protein, wherein said reporter protein affects or regulates a biological process in said cell; (c) incubating the mufti-well plate to allow cells to incorporate the first and the second vectors; (d) expressing said different test proteins and said reporter protein in a transfected cell; (e) measuring abundance and/or activity of said reporter protein by observation of an indicator of said biological process in said transfected cell, wherein said abundance and/or activity of said reporter protein is modulated by the presence of a protein that modulates said reporter protein; (f) screening said library for one or more members which encode test proteins that modulate said reporter protein; and (g) repeating (a) to (e) in an additional well of said mufti-well plate with a further cell preparation having the same or different genetic background as said first cell preparation.