Patent ID: 7527936

Claim:
A method for identifying a compound that inhibits angiogenesis, the method comprising the steps of: (i) contacting human ubiquitin hydrolase specific protease 37 (USP37) polypeptide with the compound, (ii) measuring USP37 polypeptide for ubiquitin hydrolase activity in the presence of the compound, wherein the USP37 polypeptide has ubiquitin hydrolase activity in the absence of the compound, (iii) contacting an endothelial cell with said compound identified in step (ii), (iv) measuring a change in angiogenesis phenotype in the endothelial cell by measuring VEGF-R2 surface expression, ανβ5 integrin expression, endothelial cell tube formation or haptotaxis in the presence of said compound wherein inhibition of ubiquitin hydrolase activity in the presence of said compound as compared with activity in the absence of said compound, and reduction in VEGF-R2 surface expression, ανβ5 integrin expression, endothelial cell tube formation or haptotaxis, identifies said compound as a compound that inhibits angiogenesis.