Patent ID: 7947463

Claim:
A method to quantitate immunoglobulin steroid hormone response inhibitor in a sample comprising: treating a sample to effectively remove steroid hormones from said sample; conducting an immunoglobulin steroid hormone inhibition assay by adding the treated sample to a first group of steroid-hormone responsive tumor cells which have been transferred to serum-free media or steroid hormone depleted serum, said cells being from a cultured cell line selected from the group consisting of: T47D; MCF-7A; ZR-75-1; GH3; GH1; GH4C1; or H-301; conducting an immunoglobulin steroid hormone inhibition positive control assay by adding a known amount of plasma immunoglobulin selected from the group consisting of plasma IgA or plasma IgM to a second group of said selected steroid-hormone responsive tumor cells which have been transferred to serum-free media or steroid hormone depleted serum; determining an amount of said added treated sample at which said treated sample inhibits steroid-hormone mediated cell growth in said inhibition assay; and comparing said amount of said added treated sample to said amount of plasma immunoglobulin added to said positive control assay to quantitate an amount of immunoglobulin steroid hormone response inhibitor in said treated sample.