Patent ID: 7586600

Claim:
A method of normalizing spectral sensitivity from an instrument, the instrument using a microplate having a plurality of reaction chambers, the instrument having a plurality of filters, the method comprising: depositing a first reference standard into a first of the plurality of reaction chambers in the microplate, the first reference standard outputting a first reference standard signal; depositing a sample aliquot and at least one dye into a second of the plurality of reaction chambers, the sample aliquot having at least a portion of a polynucleotide sample, the dye outputting a data signal representative of the polynucleotide sample; subjecting the sample aliquot and the first reference standard to a testing regimen, wherein the polynucleotide sample in the sample aliquot is amplified, the first reference standard signal is emitted from the first of the plurality of reaction chambers containing the first reference standard, and the data signal is emitted from the second of the plurality of reaction chambers; collecting and analyzing the first reference standard signal relative to a predetermined level to generate a normalization bias; and normalizing the data signal relative to the normalization bias.