Patent ID: 8222028

Claim:
An isolated mini-chromosome comprising a centromere, wherein the centromere comprises: (a) at least five first repeated nucleotide sequences that hybridize under conditions comprising 0.5×SSC and 0.25% SDS at 65° C. for 15 minutes, followed by a wash at 65° C. for a half hour to any one of: the nucleotide sequence of SEQ ID NO:70 or the nucleotide sequence of SEQ ID NO:71, and (b) at least a second nucleotide sequence comprising a nucleotide sequence that hydridizes under conditions comprising 0.5×SSC and 0.25% SDS at 65° C. for 15 minutes, followed by a wash at 65° C. for a half hour to any one of: the nucleotide sequence of SEQ ID NO:77 or the nucleotide sequence of SEQ ID NO:78, wherein the centromere that confers an ability to the mini-chromosome to segregate to daughter cells through cell division.