Patent ID: 6893814

Claim:
A sensor for detection of tetrameric multivalent neuraminidase within a sample, where a positive detection indicates presence of a target virus containing tetrameric multivalent neuraminidase within said sample, said sensor comprising: a surface; at least two different recognition molecules each bound through a spacer group of a defined length to an individual trifunctional linker moiety situated movably at said surface through an alkyl chain, said recognition molecules having a binding affinity with tetrameric multivalent neuraminidase, wherein at least one individual trifunctional linker moiety includes a single fluorescence donor label thereon for generating a fluorescent signal upon a predetermined binding event and at least one individual trifunctional linker moiety includes a single fluorescence acceptor label thereon; and, a means for measuring for change in fluorescent properties in response to binding between said two different recognition molecules and said tetrameric neuraminidase where a change in fluorescence indicates presence of said target virus.