Patent ID: 7629326

Claim:
A method of repairing a specific sequence comprising a I-SceI recognition site in chromosomal DNA of a cell in vitro, comprising the steps of: (a) inducing in the chromosomal DNA of the cell a double stranded cleavage by a chimeric restriction endonuclease at a site of interest in the specific sequence, said site of interest comprising a I-SceI recognition site, said chimeric restriction endonuclease comprising a I-SceI DNA binding domain and a FokI DNA cleavage domain; and (b) introducing targeting DNA into the cell under conditions appropriate for introduction of the targeting DNA into the site of interest, wherein said targeting DNA comprises (1) DNA homologous to the region surrounding the site of interest and (2) DNA which repairs the specific sequence upon recombination between said targeting DNA and the chromosomal DNA.