Patent ID: 8306757

Claim:
A method for nucleic acid sequencing, comprising: disposing a plurality of template nucleic acids in at least 10 5 reaction volumes for at least 10 5 sensors, the sensors comprising field-effect transistors having floating gates coupled to the reaction volumes to detect hydrogen ions liberated through incorporation of a nucleotide into one or more primers that are hybridized to at least one of the plurality of template nucleic acids; introducing a known nucleotide into the reaction volumes; coupling circuitry to field-effect transistors of the sensors to measure pH changes within the reaction volumes within a pre-specified pH range that is less than a maximum pH range detectable by the field-effect transistors, the measured pH change based on liberation of hydrogen ions detected by the field-effect transistors resulting from incorporation of the introduced known nucleotide into the one or more primers, and the circuitry further to generate output signals indicating the measured pH changes; and analyzing the output signals generated by the circuitry to determine at least a portion of sequences corresponding to a portion of the template nucleic acids.