Patent ID: 7842501

Claim:
A method for producing cytotoxic T cells specific to CD40L antigenic peptides comprising the steps of: (a) isolating CD8+ T cells from a subject; (b) loading antigen presenting cells having Class I MHC molecules on their surface with CD40L antigenic peptides wherein the CD40L antigenic peptides are one or more of the peptides selected from the group consisting of: FELQPGASV (SEQ ID NO:40), QLTVKRQRL (SEQ ID NO:41), QMIGSALFA (SEQ ID NO:42), SQAPFIASL (SEQ ID NO:43), ISMKIFMYL (SEQ ID NO:44), VTLENGKQLL (SEQ ID NO:45), VISEASSKT (SEQ ID NO:46), GVFELQPGA (SEQ ID NO:47), SMKIFMYLL (SEQ ID NO:48), and RQGLYYIYA (SEQ ID NO:49); (c) culturing the CD8+ T cells with the antigen presenting cells for a period of time sufficient for activation of precursor CD8+ T cells specific for the antigenic peptides; (d) expanding in culture the activated CD8+ T cells in culture; and (e) collecting CD8+ T cells from the culture.