Patent ID: 6900014

Claim:
A method for determining the presence or absence of elite event MS-B2 in Brassica plant material, said method comprising performing a polymerase chain reaction (PCR) assay on a genomic DNA sample from Brassica plant material, using an MS-B2 specific primer pair, wherein the first member of said primer pair comprises 21-23 consecutive nucleotides of nucleotides 1-234 of SEQ ID NO:8, or the complement thereof, and wherein the second member of said primer pair comprises 21-23 consecutive nucleotides of nucleotides 235-415 of SEQ ID NO:8, or the complement thereof; wherein said MS-B2 specific primer pair, when used in said PCR assay on a genomic DNA sample from transgenic Brassica plant material comprising an MS-B2 specific region, produces a MS-B2 specific DNA fragment; wherein said MS-B2 specific primer pair, when used in said PCR assay on a genomic DNA sample from non-transgenic Brassica plant material not comprising an MS-B2 specific region, does not produce said MS-B2 specific DNA fragment; wherein production of said MS-B2 specific DNA fragment in said PCR assay is indicative of the presence of elite event MS-B2 in said plant materials; and wherein no production of said MS-B2 specific DNA fragment in said PCR assay is indicative of the absence of elite event MS-B2 in said plant material.