Patent ID: 7371558

Claim:
An E. coli strain comprising: a) a disrupted endogenous phosphoenolpyruvate-glucose phosphotransferase system comprising one or more of: i) a genetically disrupted endogenous ptsH gene preventing expression of active phosphocarrier protein; ii) a genetically disrupted endogenous ptsl gene preventing expression of active phosphoenolpyruvate-protein phosphotransferase; and iii) a genetically disrupted endogenous crr gene preventing expression of active glucose-specific IIA component; b) a genetically up regulated endogenous galP gene encoding active galactose-proton symporter, said up regulation resulting in an increased galactose-proton symporter activity; wherein the up regulation is produced by a) by introducing additional copies of said gene into host cell followed by integration or b) by replacing native regulatory sequence with strong non-native promoter or altered native promoter c) a genetically up regulated endogenous glk gene encoding active glucokinase, said up regulation resulting in an increased glucokinase activity; wherein the up regulation is produced by a) by introducing additional copies of said gene into host cell followed by integration or b) by replacing native regulatory sequence with strong non-native promoter or altered native promoter, and d) a genetically down regulated endogenous gapA gene encoding active glyceraldehyde 3-phosphate dehydrogenase, said down regulation resulting in a reduced glyceraldehyde 3-phosphate dehydrogenase activity; whereby said E. coli strain is capable of bioconverting a suitable carbon source to 1,3-propanediol.