Patent ID: 7887890

Claim:
A method of manufacturing a bio-chip substrate, the method comprising: (a) preparing a plastic substrate having an Atomic Force Microscopic (AFM) surface roughness of R a <3 nm or R q <4 nm before being treated, wherein R a is an average roughness and R q is a root-mean-square roughness; (b) treating the plastic substrate with argon plasma to form a radical on the surface of the plastic substrate; and then (c) treating the plastic substrate with a surface-modifying monomer, wherein the radical is reacted with the surface-modifying monomer to form a compound on the surface of the plastic substrate, wherein the surface-modifying monomer is one of allyl-glycidylether having an epoxide group and a carbon-carbon double bond, and glycidylmethacrylate having an epoxide group and a carbon-carbon double bond, the plastic substrate being surface-modified with the epoxide group; one of hexanal, octanal, and nonanal each of which has an aldehyde group and a boiling point of 80° C. or higher, the plastic substrate being surface-modified with the aldehyde group; or one of allylamine having a primary amine group and a carbon-carbon double bond, and 3-methoxypropylamine which is an ether compound having a primary amine group, the plastic substrate being surface-modified with the amine group; and (d) preparing the plastic substrate treated in (c) as the bio-chip substrate to be used with fluorescent material-labeled biomolecules to be detected by fluorescent measurement.