Patent ID: 8293516

Claim:
A recombinant microorganism produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the recombinant microorganism is prepared by: inserting, into the genome of a parental microorganism, [i] a transcription initiation regulatory region or [ii] both the transcription initiation regulatory region and a ribosome-binding site, such that said [i] or [ii] is operably linked to the upstream of a Bacillus subtilis prsA gene or prsA gene that encodes a polypeptide with chaperone activity, thereby enhancing the expression of said prsA gene, wherein said transcription initiation regulatory region is not the original transcription initiation regulatory region of said prsA gene, or inserting, into the genome of a parental microorganism, a gene fragment prepared by operably linking a transcription initiation regulatory region or both the transcription initiation regulatory region and a ribosome-binding site to the upstream of the Bacillus subtilis prsA gene or a prsA gene that encodes a polypeptide with chaperone activity, thereby enhancing the expression of said prsA gene, wherein said transcription initiation regulatory region is not the original transcription initiation regulatory region of said prsA gene; and deleting or inactivating one or more genes selected from the group consisting of an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, and a dltE gene.