Patent ID: 8486651

Claim:
A method for identifying a potential therapeutic agent for the treatment of a disorder associated with Eyes Absent dysregulation, comprising the steps of: i) providing an Eya protein or fragment thereof, wherein the Eya protein or fragment possesses relevant biochemical activity, and a substrate capable of being dephosphorylated by the Eya protein or fragment; ii) delivering a test molecule to the Eya protein or fragment, wherein the test molecule is a small molecule, peptide, polypeptide, or antibody; iii) determining the effect of the test molecule on the phosphatase activity of the Eya protein or fragment; and iv) comparing the phosphatase activity of the Eya protein or fragment in the presence of a test molecule to that of a reference standard; wherein a test molecule that increases or decreases Eya phosphatase activity is a potential therapeutic agent for the treatment of a disorder associated with Eya dysregulation.