Patent ID: 8420323

Claim:
A nucleic acid amplification method, comprising: (a) providing a reaction solution containing (i) at least one type of deoxynucleotide triphosphate, (ii) a DNA polymerase having a strand displacement activity, (iii) a single-strand template DNA, or a double-strand template DNA including said single-strand template DNA as one strand, (iv) a forward primer that anneals to the single-strand template DNA, and (v) a reverse primer that anneals to a forward primer extension product; and (b) incubating the reaction solution to amplify the single-strand template DNA or the double-strand template DNA, wherein the forward primer includes a tag sequence at a 5′ end region, and the tag sequence is complementary to a portion of the single-stranded template DNA, said portion being present 5′ upstream of a portion of the single-stranded template DNA where a 3′ end region of the forward primer anneals.