Patent ID: 7399589

Claim:
A method for detecting or quantifying a target nucleic acid on a microarray having a plurality of sample detection sites, comprising: (a) hybridizing the target nucleic acid to a microarray-bound biomolecule probe, forming an immobilized RNA:DNA hybrid; (b) hybridizing a detectably-labeled biomolecule probe to a non-hybridized portion of the microarray-bound biomolecule probe, forming an immobilized RNA:DNA hybrid complex comprising the target nucleic acid, a detectably-labeled biomolecule probe, and a microarray-bound biomolecule probe; (c) detecting the target nucleic acid by measuring the immobilized RNA:DNA hybrid complex by binding the complex to a detectable antibody specifically reactive with an RNA:DNA hybrid; (d) detecting the detectably-labeled biomolecule probe; and (e) repeating steps (a)–(c) on each of the plurality of sample detection sites, wherein the detectably-labeled biomolecule probe is distinguishable from the detectable antibody.