Patent ID: 7977063

Claim:
A method for determining an allergic response in a subject, said method comprising: a) incubating cells capable of degranulation with an aqueous solution comprising human IgE from the subject to obtain sensitized cells; b) incubating the sensitized cells obtained in step a) with a complete nutritional composition comprising an allergen component selected from the group consisting of milk, egg and fruit, between 2 and 50 wt. % protein based on dry matter, between 10 and 90 wt. % carbohydrate based on dry matter, and optionally, between 5 and 50 wt. % lipid based on dry matter; and c) determining the extent of degranulation, wherein an extent of degranulation of at least 30% relative to the extent of degranulation of a positive control provides indication of the allergic response in the subject to the allergen.