Patent ID: 7977079

Claim:
A DNA construct encoding an engineered bovine pancreatic ribonuclease A variant, wherein said variant comprises an amino acid sequence which differs from the amino acid sequence of the native bovine ribonuclease A of SEQ ID NO: 1 solely by three or more amino acid substitutions at positions corresponding to positions of SEQ ID NO: 1 selected from the group consisting of 38, 39, 67 and 85-94, wherein at least one of the three or more amino acid substitutions is at a position corresponding to any one of positions 85-94 of SEQ ID NO: 1, wherein at least two of the three or more amino acid substitutions are at positions corresponding to positions 38, 39 or 67 of SEQ ID NO: 1, and wherein the engineered bovine pancreatic ribonuclease A variant exhibits enhanced cytotoxic activity relative to the native bovine ribonuclease A of SEQ ID NO: 1.