Patent ID: 7354753

Claim:
An isolated variant glucoamylase, wherein the variant glucoamylase comprises an amino acid sequence having at least 95% identity to SEQ ID NO:2, wherein the variant comprises an alteration of an amino acid corresponding to position 313 in the amino acid sequence of SEQ ID NO:2 and an alteration of one or more of the following positions: 59, 66, 72, 119, 189, 223, 227, 340, 342, 352, 379, 386, 393, 395, 402, 408, 416, 425, 427, 444, 486, 490, 494, and wherein the alteration is independently: (i) an insertion of an amino acid downstream of the amino acid which occupies the position, (ii) a deletion of the amino acid which occupies the position, or (iii) a substitution of the amino acid which occupies the position with a different amino acid, and wherein the variant has glucoamylase activity.