Patent ID: 8703815

Claim:
A method for assaying for the presence of transthyretin (TTR) in a sample to be assayed that comprises the steps of: a) admixing a binding/reaction sufficient amount of a covalent TTR kinetic stabilizer compound of Formula II with a sample to be assayed, wherein the depicted structure Ar is a hydrocarbyl aromatic ring structure, R 1 is a halide, a methyl, monofluoro-, difluoro- or trifluoromethyl group; R 2 is H, OH or NH 2 ; n is one; L is a linker that is HC═CH; X is a reactive substituent that reacts with an amine in an aqueous environment to bond the amine to the depicted compound that is a carboxylic ester or thioester, an azetidin-2-one, a Michael acceptor, an epoxide, aziridine or thienyl group or a □-haloacetyl group, and L and X are in a 1,3-substitution relationship on the ring to which they are bonded; and Z is H, or a second linker, L 2 , that is an X group that is the same as or different from the previously mentioned X group, a NR 3 R 4 group, where R 3 and R 4 are the same or different and are H, methyl or ethyl, a 1,3-diketo group or a metal ion chelating group; b) maintaining that admixture for a time period sufficient for said compound to bind to TTR present in the sample, react therewith and form a covalently-bound reacted compound, and c) determining the presence of said reacted compound.