Patent ID: 7816099

Claim:
A method for identifying inhibitors of ATPases or kinases catalyzing a biochemical reaction that produces ADP, comprising: (a) contacting an ATPase or kinase with a substrate and ATP in the absence of a test compound and under conditions such that at least a portion of said ATP is converted to ADP; (b) reacting the ADP with D-glucose in the presence of an ADP-dependent glucokinase, a glucose-6-P dehydrogenase, NAD(P), resazurin, diaphorase and at least 1 mM of magnesium ion, under conditions such that fluorescent resorufin is produced; (c) determining the activity of said ATPase or kinase by measuring the fluorescence emitted by said resorufin; (d) repeating step (a) in the presence of a test compounds, followed by steps (b) and (c); and (e) comparing the measurements of fluorescence in the absence and in the presence of the test compound; wherein a reduction in the fluorescence in the presence of the test compound indicates that the test compound is an inhibitor of said ATPase or kinase.