Patent ID: 6870032

Claim:
A boxed set comprising reagents for the early diagnosis of a flaviviral infection, wherein the reagents comprise: (A) a first antibody, called a capture antibody, which is unlabeled and is either (1) a polyclonal antibody with high affinity for NS1 protein of a flavivirus in hexameric form, wherein the polyclonal antibody is preselected by immunocapture with the NS1 protein in hexameric form or (2) a mixture of purified anti-NS1 monoclonal antibodies with high affinity for the NS1 protein of the flavivirus in hexameric form, wherein the monoclonal antibodies are preselected by immunocapture with NS1 protein in hexameric form; (B) a second antibody, called a revelation antibody, which is either (1) a polyclonal antibody directed against NS1 protein in hexameric form or (2) a mixture of monoclonal antibodies directed against NS1 protein in hexameric form, wherein the revelation antibody is conjugated to a label; (C) at least one positive control comprising an NS1 protein of a flavivirus, wherein the NS1 protein is in hexameric form; and, (D) at least one negative control comprising an uninfected human serum.