Patent ID: 7579171

Claim:
A method of generating ascorbic acid, comprising: a) obtaining a recombinant yeast capable of converting an ascorbic acid precursor into ascorbic acid, wherein the yeast is functionally transformed with a coding region encoding a first enzyme selected from D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO), or L-gulono-1,4-lactone oxidase, b) culturing the recombinant yeast in a medium comprising an ascorbic acid precursor, thereby forming ascorbic acid, and c) isolating the ascorbic acid, wherein the yeast is selected from S. cerevisiae strain GRF18U; S. cerevisiae strain W3031B; K. lactis strain PM6-7A; or Z. bailii strain ATCC 60483.