Patent ID: 7704694

Claim:
A method for detecting species in the genus Glycine in cases where even one species of the genus Glycine is contained in a subject to which the method is to be applied such as a food ingredient or product, which comprises the steps of: a) collecting 45S rRNA precursor gene sequences containing an ITS sequence of the genus Glycine and plants thought to be related species thereof and then selecting the region common to the genus Glycine in the 45S rRNA precursor gene sequences; b) selecting the base specific to the genus Glycine from the region; c) designing primers (A) and/or (B) having the specific base at the 3′ end thereof, which primers can hybridize under the stringent conditions to a nucleic acid molecule having a common nucleotide base sequence for all species in the genus Glycine in the 45S rRNA precursor gene sequence thereof, wherein the 3′ end of primer (A) can complementarily bind to a base in the ITS-1 Sequence of the genus Glycine when the primer hybridizes to the nucleic acid molecule while the 3′ end of primer (B) can complementarily bind to a base in the ITS-2 sequence of the genus Glycine when the primer hybridizes to the nucleic acid molecule; d) isolating DNA from the subject to which the method is to be applied; e) conducting PCR amplification for the isolated DNA using at least one member selected from the group consisting of the primers (A) and (B); and f) identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the genus Glycine.