Patent ID: 8394591

Claim:
A method for the generation of a physical map of at least part of a genome comprising the steps of: (a) providing a sample DNA; (b) generating an artificial chromosome clone bank wherein each artificial chromosome clone contains part of the sample DNA; (c) combining the artificial chromosome clones in a plurality of pools, wherein each clone is present in more than one pool of the plurality of pools, to create a library; (d) digesting the DNA of the clones in the plurality of pools with one or more restriction endonucleases to provide for a set of restriction fragments for each pool; (e) ligating adaptors to one or both sides of the restriction fragments, wherein at least one adaptor contains a pool-specific identifier or a degenerate identifier section, respectively, to provide adaptor-ligated restriction fragments; (f) determining the sequence of at least the pool-specific identifier and part of the restriction fragment of the adapter-ligated restriction fragments; (g) assigning the restriction fragment sequences determined in the adapter-ligated restriction fragments to the corresponding clones using the pool-specific identifiers; (h) building a contig based on sequence matching of the restriction fragment derived sections; (i) ordering the restriction fragments of step (h) to thereby build a clone-contig and generating a physical map.