Patent ID: 8173370

Claim:
A multiplex method for determining the presence or absence of one or more RhD exons in a fetal nucleic acid, comprising: simultaneously amplifying fetal nucleic acid from a pregnant female with two or more amplification primers yielding amplification products, contacting the amplification products with extension primers under extension conditions, thereby generating extension products, and determining the presence or absence of an RhD exon in the fetal nucleic acid based on the extension products; wherein: the extension primers independently comprise a nucleotide sequence of; gGATAAAGATCAGACAGCAAC (SEQ ID NO: 16) cTGCAGACAGACTACCACATGAAC (SEQ ID NO: 18) tTGTCACCACGCTGACTGCTA and (SEQ ID NO: 21) CTTGCTGGGTCTGCTTGGAGAGATCA (SEQ ID NO: 22) and a nucleotide in lowercase text in the table is not complementary to a RhD sequence nucleotide.