Patent ID: 8227213

Claim:
A method for transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage from a N-acetyl-D-galactosamine donor substrate to an acceptor substrate containing N-acetyl-D-glucosamine, which comprises reacting the N-acetyl-D-galactosamine donor substrate, the acceptor substrate containing N-acetyl-D-glucosamine, and a polypeptide selected from the group consisting of the following: i) a polypeptide consisting of the amino acid sequence shown in SEQ ID NO: 2; ii) a polypeptide having the amino acid sequence covering amino acids 36 to 500 shown in SEQ ID NO: 2; iii) a polypeptide having the amino acid sequence covering amino acids 189 to 500 shown in SEQ ID NO: 2; iv) a polypeptide consisting of an amino acid sequence sharing at least 90% identity with the amino acid sequences described in i) to iii) described above, which transfers N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage; and v) a polypeptide encoded by a nucleic acid sequence that hybridizes with the nucleic acid sequence shown in SEQ ID NO: 1 under high stringency conditions of hybridization at 50° C., 2 ×SSC, and 50% formamide and washing at 68° C., 0.2 ×SSC, and 0.1% SDS, which transfers N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage.