Patent ID: 8029991

Claim:
A method for the isolation of a nucleic acid from a starting material containing a nucleic acid, comprising: digesting the starting material with a lysis buffer which contains neither a chaotropic nor an antichaotropic component, to obtain a digested component containing said nucleic acid; optionally, preliminarily filtering said digested component containing said nucleic acid; adding of a member selected from the group consisting of (i) an alcohol, (ii) a detergent and (iii) a mixture of an alcohol and a detergent; binding the nucleic acid to a solid phase, to obtain a bound nucleic acid; washing the bound nucleic acid with a wash buffer; eluting of the bound nucleic acid with a wash buffer, an elution buffer, a combination thereof, or with water, to obtain said nucleic acid; wherein said alcohol is added and wherein said alcohol is water-soluble; wherein said alcohol comprises at least one salt of a polyvalent cation.