Patent ID: 6998516

Claim:
A method for producing transformed sunflower cotyledons comprising: obtaining a cotyledon from a germinated sunflower seedling; incubating the cotyledon at a temperature between about 0° C. and about 10° C.; contacting the cotyledon with a culture of Agrobacterium in an infiltration media comprising 6-benzylaminopurine, one or more cytokinins, and one or more carbohydrates; culturing the Agrobacterium -contacted cotyledon in a first media to produce transformed cotyledon tissue, wherein the first media has a high osmotic pressure and said first media comprises at least one carbohydrate selected from the group consisting of glucose, sucrose, mannitol, fructose, maltose, mannose, or xylose at a concentration of about 5% (w/v) to about 30% (w/v); inducing shoot growth from the transformed cotyledon tissue in a second media, wherein the second media has a low osmotic pressure; and incubating the transformed cotyledon tissue thus produced in a selection media comprising at least one selection agent.