Patent ID: 8173384

Claim:
A method for analyzing a heparin sample, comprising: contacting the sample with heparinase I, heparinase II and heparinase III; determining an amount of a signature component in the sample, and comparing the amount of the signature component in the sample to a reference database for a heparin to analyze an activity of the heparin sample, wherein the signature component is selected from the group consisting of: ΔUH NAc,6S GH NS,3S,6S ; ΔUH NS,6S GH NS,3S,6S ; ΔUH NAc,6S GH NS,3S ; ΔUH NS,6S GH NS,3S ; ΔU 2S H NS,6S I 2S H NS,6S I 2S H NS,6S IH NAc,6S GH NS,3S,6S ; ΔU 2S H NS,6S IH NS,6S I 2S H NS,6S IH NAc,6S GH NS,3S,6S ; ΔU 2S H NS,6S GH NS,6S I 2S H NS,6S IH NAc,6S GH NS,3S,6S ; ΔU 2S H NS,6S I 2S H NS,6S I 2S H NS,6S IH NAc,6S GMan 3S,6S ; I 2S H NS,6S I 2S H NS,6S IH NAc,6S GH NS,3S,6S ; ΔU 2S H NS,6S I 2S H NS,6S IH NAc,6S GH NS,3S,6S ; I 2S H NS,6S I 2S H NS,6S IH NAc,6S GMan 3S,6S ;ΔU 2S H NS,6S IH NAc,6S GH NS,3S,6S ;I NS,6S IH NAc,6S GH NS,3S,6S ; ΔU 2S H NS,6S I 2S H NS,6S ; ΔU 2S H NS,6S ,IH NS,6S ; ΔU 2S H NS,6S GH NS,6S ; ΔU 2S H NS,6S I 2S Man 6S ; IH NAc,6S GMan 3S,6S ; H NAc,6S GMan 3S,6S ; H NS,6S GH NS,3S,6S I 2S H NS,6S,OMe ; H NS,6S GH NS,6S I 2S H NS,6S,OMe ; ΔUH NS,6S I 2S H NS,6S,OMe ; H NS,6S GH NS,6S ; H NS,6S GH NS,3S,6S ; ΔU 2S H NS,6S,OMe ; ΔU 2S H NS,6S ; ΔU 2S H NS ; ΔUH NS,6S ; ΔU 2S H NAc,6S ; ΔUH NS ; ΔU 2S H NAc ; ΔUH NAc,6S and ΔUH NS,3S,6S .