Patent ID: 8206977

Claim:
A library of tricistronic prokaryotic display vector constructs comprising: a regulatable prokaryotic promoter; a first nucleic acid sequence encoding a gIIp phage coat protein or C-terminal domain thereof; a second nucleic acid sequence encoding a first immunoglobulin (Ig) polypeptide; a third nucleic acid sequence encoding a second immunoglobulin (Ig) polypeptide; a nucleic acid sequence encoding a first associating agent fused to or comprised within said nucleic acid sequence encoding the gIIp phage coat protein or C-terminal domain thereof, wherein said first associating agent comprises a cysteine residue; and a nucleic acid sequence encoding a second associating agent fused to or comprised within said nucleic acid encoding the first immunoglobulin (Ig) polypeptide, wherein said second associating agent comprises a cysteine residue, wherein said first, second and third nucleic acid sequences are under the control of said promoter, and wherein upon expression of said tricistronic vector, (i) said gIIIp phage coat protein or C-terminal domain thereof and said first Ig polypeptide associate via their respective associating agents and (ii) said first and second Ig polypeptides self-associate.