Patent ID: 7393664

Claim:
A method for producing a secreted polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence, the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence, and the first, nucleotide sequence is selected from the group consisting of, (i) a nucleotide sequence encoding a signal peptide comprising or consisting of the amino acid sequence of SEQ ID NO: 37; and (ii) a nucleotide sequence comprising or consisting of SEQ ID NO: 36; and (b) isolating the secreted polypeptide from the cultivation medium, wherein the fungal host cell produces at least about 25% more secreted polypeptide relative to the fungal host cell comprising a native signal peptide sequence operably linked naturally to the second nucleotide sequence encoding the polypeptide when cultured under identical production conditions.