Patent ID: 8828701

Claim:
An isolated variant of a parent cellobiohydrolase, comprising a substitution at one or more positions corresponding to positions 254, 285, 286, 330, 342, and 360 of SEQ ID NO: 2, wherein the variant has cellobiohydrolase activity and wherein the parent cellobiohydrolase is selected from the group consisting of: (a) a polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) the mature polypeptide genomic coding sequence of SEQ ID NO: 1, (ii) the mature polypeptide cDNA coding sequence contained in SEQ ID NO: 1, or (iii) the full-length complementary strand of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.; (c) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide genomic coding sequence of SEQ ID NO: 1, or the mature polypeptide cDNA coding sequence contained in SEQ ID NO: 1; and (d) a fragment of the mature polypeptide of SEQ ID NO: 2, which has cellobiohydrolase activity.