Patent ID: 7338789

Claim:
A method for in vitro transcription of mRNA and/or translation of polypeptides, the method comprising: synthesizing said mRNA and/or polypeptides in a transcription and/or translation reaction mix substantially free of polyethylene glycol, comprising: an extract from E. coli cells comprising membrane vesicles containing respiratory chain components; components of polypeptide and/or mRNA synthesis machinery; a template for transcription of said mRNA and/or translation of said polypeptide; monomers for synthesis of said mRNA and/or polypeptides; and co-factors, enzymes and other reagents necessary for said transcription and/or translation; magnesium at a concentration of from about 5 mM to about 20 mM; wherein oxidative phosphorylation, which is sensitive to electron transport chain inhibitors, is activated in said reaction mix.