Patent ID: 8071341

Claim:
A yeast cell comprising docosapentaenoic acid and docosahexaenoic acid in an esterified form as part of triacylglycerols in the yeast cell; a polynucleotide which encodes a Î”5 elongase operably linked to a promoter which directs expression of the polynucleotide in the yeast cell; a polynucleotide which encodes a Î”4 desaturase operably linked to a promoter which directs expression of the polynucleotide in the yeast cell; an exogenous polynucleotide which encodes a desaturase which desaturates an acyl-CoA substrate in a yeast cell; and a total fatty acid content in the yeast cell which includes 2.5% (w/w) C20 Ï‰3 long-chain polyunsaturated fatty acids (LC-PUFAs) and 0.1% (w/w) docosapentaenoic acid.