Patent ID: 7217507

Claim:
A method of determining a property of a target that binds to a ligand, which method comprises: (i) providing one or more ligands, (ii) immobilizing each of the ligands in a first matrix, wherein each of the ligands has a different position within the first matrix, (iii) contacting the ligands within the first matrix with one or more targets wherein at least one target binds to at least one ligand, thereby forming one or more target-ligand complexes within the first matrix, (iv) transferring in a transfer buffer, at least a portion of the target of at least one target-ligand complex within the first matrix to a second matrix so that the ligand of the target-ligand complex remains in the first matrix, wherein the position of the target within the second matrix identifies the position of the ligand within the first matrix, and (v) detecting the target on the second matrix, whereupon a property of the target that binds to a ligand is determined, wherein said property is a biological property, a chemical property, a physical property, a biochemical property or a property which is any combination thereof.