Patent ID: 7897366

Claim:
A method for simultaneously expressing a cofactor regenerating enzyme and a target protein using the cofactor on the cell surface, the method comprising the steps of: (a) preparing a recombinant vector for regenerating the cofactor comprising DNA having a gene encoding an enzyme for regenerating the cofactor linked to a gene encoding outer membrane protein C (OmpC) so that the enzyme regenerating the cofactor is expressed in the form fused with OmpC; (b) preparing a recombinant vector for expressing the target protein comprising DNA having a gene encoding the target protein linked to a gene encoding OmpC so that the target protein using the cofactor is expressed in the form fused with OmpC; (c) transforming the recombinant vector for expressing the target protein and the recombinant vector for regenerating the cofactor into E. coli ; and (d) surface expressing the enzyme regenerating the cofactor and the target protein by culturing the transformed cell.