Patent ID: 7541429

Claim:
An isolated peptide ligand for an individual class I MHC molecule, the peptide consisting of a sequence selected from the group consisting of SEQ ID NOS: 29-32 and 34-41 and being isolated by a method comprising the steps of: providing a cell line containing a construct that encodes an individual soluble class I MHC molecule, the cell line being able to naturally process proteins into peptide ligands capable of being loaded into antigen binding grooves of class I MHC molecules; culturing the cell line under conditions which allow for expression of the individual soluble class I MHC molecules from the construct, such conditions also allowing for endogenous loading of a peptide ligand into the antigen binding groove of each individual soluble class I MHC molecule prior to secretion of the individual soluble class I MHG molecules from the cell; isolating the secreted individual soluble class I MHC molecules having the endogenously loaded peptide ligands bound thereto; and separating the peptide ligands from the individual soluble class I MHC molecules.