Patent ID: 8796200

Claim:
A method of preparing a contrast agent for medical use comprising the steps of: providing an adhesin comprising an amino acid sequence selected from the group consisting of the following sequences, DraE SEQ ID NO: 1 wherein said adhesin has one or more of the following mutations and is deleted N-terminally by up to 18 amino acids and/or C-terminally by up to 10 amino acids: T7 (N, F, C, S, V, R, A, I, L, Y); E17 (S, P, K, G, D, R, N, H, Q); R22 (T, A, S, N, K); D25 (S, G, N, A, T, K, R, H, Q, M); T27 (K, R, L, V, Y, P, N, O); V28 (W, F); A29 (K, R, S, E, Q, F, G, L, H, P, N, T, W); T31 (G, D, S, N); Q34 (D, G, S, N, L, V, T, A); D37N; A38 (S, T, L); A39 (Q, S, D, M, G, F); I41 (V); Q47 (T, N, C, S, G, A, P); D52 (G, N, S, C, P, Q, Y, H, K, R, T); N84 (D, S, H); R86V; T88 (M, L); T95 (L, M, Y, F, C, W, Q, N, E, S, I, H); F100 (Y, V); V105 (S, A, T, R, M, V, P, N, E, Q, G, K, H); I111 (C, V, H, Y, T, M, F) I114 (V, L, A, C); Y115 (T, W, E, V); V116 (A, S, L); G118 (P, S), combining the adhesin with a suitable carrier for use as a contrast agent in medical treatment.