Patent ID: 7892797

Claim:
A method for PCR amplification of long nucleic acid target region sequences, which method comprises: (a) providing a nucleic acid sample including the amplification target; (b) adding oligonucleotide primers, a single thermally stable DNA polymerase of Tba DNA polymerase or Tba exo* DNA polymerase and deoxynucleoside triphosphates to form a reaction mixture; and (c) incubating said reaction mixture under thermal cycling conditions to promote amplification of said target by extension of primers to form multiple amplified PCR products using an extension time, in minutes, less than a quarter of the length of the largest PCR product in kb (¼×length of PCR product); wherein said amplification is performed in the absence of other enzymes or proteins or peptides, and further wherein said amplified PCR product is 10-50 kb in length.