Patent ID: 6911204

Claim:
A method for treating a B cell mediated malignancy in a patient, said method comprising: administering to said patient a composition comprising two chimeric proteins; wherein (1) the first chimeric protein comprises at least a portion of a V H region and at least a portion of an immunoglobulin constant region, (2) the second chimeric protein comprises at least a portion of a V L region and at least a portion of an immunoglobulin constant region, (3) wherein said V H and said V L region are isolated from a malignant B cell clone from said patient having said B cell mediated malignancy; and (4) wherein said chimeric proteins are produced in insect cells by a baculovirus expression vector wherein (a) the gene encoding said first chimeric protein is operatively linked to an AcNPV p10 promoter and a honey bee melittin secretory signal sequence, or is operatively linked to an AcNPV polyhedrin promoter and a human placental alkaline phosphatase secretory signal sequence; and (b) the gene encoding said second chimeric protein is operatively linked to an AcNPV p10 promoter and a honey bee melittin secretory signal sequence, or is operatively linked to an AcNPV polyhedrin promoter and a human placental alkaline phosphatase secretory signal sequence; and wherein said first and said second chimeric proteins are not operatively linked to the same promoter.