Patent ID: 8216789

Claim:
A method for detection of a malignancy associated with breast cancer in a test specimen of bodily fluid, the method comprising: (a) contacting the specimen with at least two antigens selected from the group consisting of p53, IGFBP2, Topo2α, cathepsin D, cyclin B, cyclin D1, MUC1, HER-2/neu and CEA; (b) incubating the specimen and the antigens for a duration and under conditions that are sufficient for the formation of immunocomplexes; (c) detecting the presence or absence of immunocomplex formation between the antigens and antibodies specific for the antigens in the specimen, and (d) determining the presence or absence of the malignancy, wherein presence of immunocomplex formation is indicative of malignancy; and wherein the at least two antigens comprise p53, cyclin B, HER-2/neu and CEA.