Patent ID: 8669075

Claim:
A method for immobilization of a vascular endothelial growth factor (VEGF) on a solid substrate comprising: performing PCR reaction with PCR primers of SEQ ID NO: 1 and SEQ ID NO:2 to amplify a nucleic acid sequence encoding VEGF; ligating together the amplified nucleic acid sequence encoding VEGF to a nucleic acid sequence encoding maltose binding protein (MBP) to make a nucleic acid sequence encoding a fusion protein of VEGF and MBP, wherein the fusion protein is a VEGF linked to a carboxyl terminal of MBP; preparing the fusion protein from the nucleic acid sequence encoding the fusion protein; and immobilizing the fusion protein on the hydrophobic surface by physical adsorption of amino terminal containing hydrophobic domain exposed on the surface of MBP on the hydrophobic surface of a solid substrate.