Patent ID: 8105767

Claim:
A transgenic buffalo green monkey kidney cell line expressing human decay accelerating factor (BGMK-hDAF), obtained by a method comprising: a) transfecting a plurality of buffalo green monkey kidney cells (BGMK) with a vector comprising a sequence encoding human decay accelerating factor (hDAF); b) contacting said transfected BGMK cells with a selection medium; and c) selecting transfected BGMK-hDAF cells to obtain a stable transgenic BGMK-hDAF cell line which has a property selected from the group consisting of i) increased sensitivity of detection of one or more enteroviruses compared to a control BGMK cell lacking expression of said hDAF, and ii) permissiveness to echovirus selected from the group consisting of echovirus-6 and echovirus-11; wherein said one or more enteroviruses include polioviruses, Coxsackie A viruses, Coxsackie B viruses, echoviruses, and enterovirus types 68, 69, 70 and 71.