Patent ID: 7883861

Claim:
A high-throughput assay system for rapidly identifying a population of primitive hematopoietic cells having a proliferative status suitable for transplantation into a patient, comprising: (a) a target cell population comprising primitive hematopoietic cells; (b) a cell growth medium comprising serum and methyl cellulose; (c) a proliferation agent comprising one or more growth factors, one or more cytokines, or combinations thereof; (d) a reagent capable of reacting with ATP and generating luminescence in the presence of ATP; and (e) a plate; wherein the target cell population, the cell growth medium, the proliferation agent, and the reagent capable of reacting with ATP and generating luminescence in the presence of ATP are combined in an order to determine the proliferative status of the target population of primitive hematopoietic cells by detecting the level of luminescence generated from the reagent that reacted with the ATP, and wherein the level of luminescence detected indicates the amount of ATP in the population of primitive hematopoietic cells, and the amount of ATP in the population of primitive hematopoietic cells indicates the proliferative status of the population of primitive hematopoietic cells and the suitability of the cell population for transplantation into a recipient patient.