Patent ID: 7309587

Claim:
A process for producing an N-acetylglucosamine-containing complex carbohydrate, which comprises: allowing (a) a culture of a transformant capable of producing a protein selected from the group consisting of (i) a protein comprising the amino acid sequence of SEQ ID NO:1 and (ii) a protein which has a homology of at least 95% with the amino acid sequence of SEQ ID NO:1, and which has β1,3-N-acetylglucosaminyltransferase activity, or a treated culture of the transformant as an enzyme source, (b) uridine 5′-diphosphate N-acetylglucosamine and (c) an acceptor complex carbohydrate comprising an oligosaccharide having galactose in its non-reducing terminal to be present in an aqueous medium, producing and accumulating the N-acetylglucosamine-containing complex carbohydrate in the aqueous medium, and recovering the N-acetylglucosamine-containing complex carbohydrate from the aqueous medium, wherein the treated culture is selected from the group consisting of a concentrated culture, a dried culture, cells obtained by centrifuging the culture, dried cells and freeze-dried cells which comprise whole cells and has the enzymatic activity of the culture.