Patent ID: 6861512

Claim:
A process for separating cis and trans isomers in a mixture of geometric isomers of an olefinic compound, the process comprising: (a) flowing a mobile phase through a column means containing a stationary phase comprising an organosilane having a pendant aliphatic functional group attached to a solid support, the mobile phase comprising an aliphatic hydrocarbon and a mixture of cis and trans isomers of a compound of the formula: where R 1 is selected from the group consisting of where each J, K and Q, independently, is straight or branched C1 to C15 alkyl; L is O, NH or CH 2 ; M is O or NH; and G is NH, O, S, SO, or SO 2 ; R 2 is straight or branched C5 to C15 alkyl; R 3 is selected from the group consisting of where E is N, O, S, SO, or SO 2 ; each A, B and D, independently, is straight or branched C1 to C15 alkyl; R 4 is selected from the group consisting of straight or branched C4 to C20 alkyl, and where each U and V, independently, is straight or branched C2 to C15 alkyl and W is hydrogen or straight or branched C1 to C5 alkyl; R 5 is selected from the group consisting of hydrogen, J′,-J′-OH,-J′-O—K′,-J -O—K′-OH, and -J′-O—PO(OH) 2 , where each J′ and K′, independently, is straight or branched C1 to C5 alkyl; R 6 is selected from the group consisting of hydroxy, halogen C1 to C5 alkoxy and C1 to C5 acyloxy; A 1 and A 2 , independently, are selected from the group consisting of where Z is straight or branched C1 to C10 alkyl; or pharmaceutically acceptable salts thereof, wherein constituents of the mobile phase preferentially interact with the stationary phase and produce an eluent stream, a first portion of which is substantially comprised of the cis isomer and a second portion of the eluent stream which is substantially comprised of the trans isomer; (b) collecting a first portion of the eluent stream substantially comprising the cis isomer; and (c) collecting a second portion of the effluent stream, substantially comprising the trans isomer.