Patent ID: 7910323

Claim:
A method of identifying the effect of a drug agent on a rate of in vivo metabolism of one or more sugars or fatty acids in an individual, the method comprising: (a) administering a drug agent to the individual; (b) administering a known quantity of 2 H-labeled sugars or 2 H-labeled fatty acids to an individual for a sufficient time for the 2 H-labeled sugars or 2 H-labeled fatty acids to be metabolized in the individual to produce 2 H-labeled water; (c) obtaining one or more bodily tissues or fluids at one or more times from the individual, wherein the one or more bodily tissues or fluids comprise a portion of the 2 H-labeled water; (d) determining a quantity of 2 H present in the portion of the 2 H-labeled water to quantify an amount of 2 H released into body water; and (e) calculating a ratio of the amount of 2 H released into body water to the known quantity of 2 H administered as 2 H-labeled sugars or 2 H-labeled fatty acids to determine the rate of in vivo metabolism of the one or more of sugars or fatty acids in the individual to whom the drug agent has been administered; and (f) comparing the rate of in vivo metabolism of the one or more sugars or fatty acids in the individual to whom the drug agent has been administered to the rate of in vivo metabolism of the one or more sugars or fatty acids in an individual to whom the drug has not been administered to identify the effect of the drug agent on the rate of in vivo metabolism of one or more sugars or fatty acids in an individual.