Patent ID: 8470600

Claim:
A cell or tissue culture container for promoting undifferentiated cell growth of undifferentiated pluripotent mammalian embryonic stem cells, the container having a surface for exposure to a culture during use, where at least a part of the surface comprises a biocompatible material, wherein at least a part of an exposed face of the biocompatible material has a nano- and/or micrometer scale topographical structure comprising a plurality of protrusions arranged on grid points of a regular two-dimensional grid, wherein the cross-sectional dimensions of the protrusions occupy an area equal to or less than 25% of the total area of the structure, said area being measured in the plane of the exposed face of the structure, and wherein the density of protrusions is equal to or greater than 1 protrusions per 65 μm 2 , and wherein each of the protrusions of said topographical structure has a vertical height/depth dimension of about 2.4 μm and the maximum gap between any protrusions and its nearest neighbor (d;Y) is between about 2 μm-6 μm.