Patent ID: 8518671

Claim:
A method for detecting a target nucleic acid in a sample comprising: a) synthesizing a nucleic acid comprising a copy of the target nucleic acid or a complement of the target nucleic acid using a reaction mixture that comprises (i) a first primer comprising a tagging sequence and a sequence complementary to a first sequence of the target nucleic acid or the complement of the target nucleic acid, (ii) a second primer comprising at least one non-natural base and a sequence complementary to a second sequence of the target nucleic acid or the complement of the target nucleic acid, and (iii) a labeled, non-natural nucleotide triphosphate having a non-natural base that is complementary to the non-natural base of the second primer; b) contacting the synthesized nucleic acid with an oligonucleotide bound to a solid support, wherein the oligonucleotide comprises a molecular recognition sequence that is complementary to the tagging sequence of the first primer; and c) detecting specific hybridization of the synthesized nucleic acid and the oligonucleotide bound to the solid support, thereby detecting the target nucleic acid.