Patent ID: 7776554

Claim:
A method of detecting a ligand that reacts with a receptor immobilized on polydiacetylene from a sample, the method comprising steps of: i) mixing a diacetylene monomer represented by formula I below and 20-30 mol % of a diacetylene monomer represented by formula II to form a polydiacetylene supramolecule of a liposome, a micelle, a Langmuir Blodgett film or a Langmuir Schaeffer film; ii) immobilizing the receptor on the supramolecule by employing the thiol functional group of the receptor; iii) polymerizing the supramolecule by irradiating UV light on the supramolecule of the above step ii); and iv) reacting the polymerized supramolecule of the above step iii) with the sample, wherein a color transition indicates the presence of the ligand in the sample, and wherein formula I and formula II are as follows: wherein R 1 is a C 1 to C 10 alkyl group, R 2 is a C 1 to C 10 alkyl group, R 3 is wherein n represents an integer of 1 to 10, and R 4 is