Patent ID: 8809006

Claim:
An in vitro method for determining thrombin potential and plasmin potential generated in a single enzymatic reaction mixture in a single well, the method comprising: adding (i) a trigger molecule for inducing thrombin generation; (ii) a thrombin-specific substrate, which, upon cleavage by thrombin, produces a measurable, thrombin-specific signal; (iii) a trigger molecule for inducing plasmin generation; (iv) a plasmin-specific substrate, which, upon cleavage by plasmin, produces a measurable, plasmin-specific signal; (v) a phospholipid-containing surface; and (vi) calcium ions; to a test sample comprising a blood product, thereby generating the single enzymatic reaction mixture; simultaneously measuring thrombin-specific and plasmin-specific signals in the single enzymatic reaction mixture in the single well; and determining the thrombin potential and the plasmin potential generated in the single enzymatic reaction mixture in the single well.