Patent ID: 7750119

Claim:
A method of producing substantially pure 2S canola protein, consisting the steps of: solubilizing canola proteins from canola oil seed meal with an aqueous saline solution having a pH of about 5 to 6 and under salt concentration of about 0.25 to 0.35 M which favors selective binding of 2S canola protein to a cation-exchange medium to form a canola protein solution, separating the canola protein solution from residual canola oil seed meal, contacting the canola protein solution with the cation-exchange medium under conditions wherein the 2S canola protein is bound to the cation-exchange medium in preference to other canola proteins, separating the bound 2S canola protein from unbound canola proteins and impurities, and separating the bound 2S canola protein from the cation-exchange medium.