Patent ID: 8877446

Claim:
A method for detecting interaction between a bait protein and a prey protein in cells, comprising the steps of: (i) providing cells comprising a first construct and a second construct, wherein the first construct comprises a polynucleotide encoding a first fusion protein which comprises the bait protein, a first fluorescent protein and a CBD (cellulose-binding domain) protein, and wherein the second construct comprises a polynucleotide encoding a second fusion protein which comprises the prey protein and a second fluorescent protein; (ii) expressing the fusion proteins and allowing formation of inclusion bodies in the cells; and (iii) measuring fluorescence co-localization of the first and second fluorescent proteins in the inclusion bodies in the cells, wherein the fluorescence co-localization in the inclusion bodies of the cells indicates the interaction of the bait protein and the prey protein; and wherein the second fusion protein, when expressed in the absence of the first fusion protein, does not form inclusion bodies in the cells, and wherein the CBD protein facilitates formation of inclusion bodies.