Patent ID: 7326567

Claim:
A plasmid system comprising in separate containers: (a) a first universal transfer vector comprising the following, first multiple cloning site, which multiple cloning site comprises the following restriction sites: Bss HII, Pme I, Sna B1, Hin dIII, Asp 718, Kpn I, Pae R71, Xho I, Sal I, Acc I, Hinc II, Cla I, Eco RV, Eco RI, Pst I, Eco O109I, Eco O109I, Apa I, Xma I, Bsp EI, Bam H1, Dsa I, Eag I, Ecl XI, Not I, Sac II, Xma III, Xba I, Sac I, Mlu I, Bcl I, Bsr GI, Bss HII; wherein said restriction sites are in said orientation or in a reverse orientation; (b) a second universal transfer vector comprising the following, second multiple cloning site, which multiple cloning site comprises the following restriction sites: Bss HII, Sgr AI, Xma I, Rsr II, Spe I, Sna B1, Hin dIII, Asp 718, Kpn I, Pae R71, Xho I, Sal I, Acc I, Hinc II, Cla I, Eco RV, Eco RI, Pst I, Eco O109I, Eco O109I, Apa I, Bsp EI, Bam H1, Dsa I, Eag I, Ecl XI, Not I, Sac II, Xma III, Xba I, Sac I, Nde I, Msc I, Nru I, Pac I, Bss HII; wherein said restriction sites are in said orientation or in a reverse orientation; and (c) an amplifiable vector comprising the following, third multiple cloning site, which multiple cloning site comprises the following restriction sites: Sgr AI, Srf I, Xma I, Spe I, Sac II, Rsr II, Pac I, Nru I, Not I, Nde I, Msc I, Mlu I, Kpn I, Fse 1, Bss HII, Bsr GI, Bsp EI, Bcl I, Bbv C1, Pme I, Bss HII, Asc I, Xba I; wherein said restriction sites are in said orientation or in a reverse orientation; and wherein the first universal transfer vector, the second universal transfer vector and the amplifiable vector are in separate containers.