Patent ID: 7208283

Claim:
An in vitro method of determining if a test substance has an androgenic or anti-androgenic effect, said method comprising the steps of: a) exposing cells, which recombinantly express Ewing sarcoma protein (EWS) of SEQ ID NO: 2 or a fragment of said Ewing sarcoma protein comprising amino acids 319–656 and which express human androgen receptor (AR) or a fragment of said human androgen receptor comprising amino acids 325–918 of SEQ ID NO: 8, to said test substance to be tested in vitro; and b) measuring protein-protein interaction or protein-protein-DNA interaction in order to determine the effect of the test substance on binding of said Ewing sarcoma protein (EWS) or said fragment of said Ewing sarcoma protein with said human androgen receptor (AR) or said fragment of said human androgen receptor; wherein the androgenic or anti-androgenic effect of the test substance is indicated by an increase or decrease in the binding determined in step b) in the presence of the test substance in comparison to the binding without the test substance present.