Patent ID: 8268554

Claim:
A method for detecting an analyte in a sample, comprising: (a) contacting said sample with at least one set of at least first, second and third proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte, the nucleic acid domain of said third proximity probe being a splint which is capable of hybridizing at least to the nucleic acid domains of said first and second proximity probes, wherein when all of the at least three proximity probes bind to said analyte, the nucleic acid domains of said first and second proximity probes are directly or indirectly conjugatable by means of a ligation reaction templated by said hybridized splint of said third proximity probe; (b) conjugating the nucleic acids of said first and second proximity probes; and (c) detecting said conjugation thereby detecting said analyte.