Patent ID: 8470379

Claim:
A method of treating a disease associated with Helicobacter pylori in a patient comprising inhibiting the growth of Helicobacter pylori in the stomach of the patient by administering to the patient a therapeutically effective amount of a product prepared from rhizomes of Zingiber officinale , said product being prepared by a process comprising the following steps: a) preparing a crude extract from rhizomes of Zingiber officinale , said crude extract comprising 6-gingerol and 6-shogaol; b) introducing the crude extract to a normal phase chromatography column, and eluting the column with a first eluent, said first eluent having a polarity lower than that of a mixture of n-hexane and ethyl acetate in a weight ratio of 6:4, so that a first eluate resulting from elution of the first eluent is obtained; and c) removing the first eluent from the first eluate by evaporation, so that a first concentrated eluate is obtained and is used as the product; wherein step a) comprises steps i) to iv), or comprises step I), step I′), or step I″), wherein said steps i) to iv) are: i) shedding fresh rhizomes of Zingiber officinale and filtering the resulting mixture to obtain a filtrate and a residue; ii) extracting the filtrate with a first organic solvent, recovering the resulting extraction solution of the first organic solvent, and evaporating the first organic solvent from the extraction solution to obtain a first concentrated extraction solution; iii) extracting the residue with a second organic solvent, recovering the resulting extraction solution of the second organic solvent, and evaporating the second organic solvent from the extraction solution to obtain a second concentrated extraction solution; and iv) combining the first concentrated extraction solution and the second concentrated extraction solution to obtain the crude extract; said step I) is: I) extracting dried rhizomes of Zingiber officinale with an organic solvent, recovering the resulting extraction solution of the organic solvent, and evaporating the organic solvent from the extraction solution to obtain the crude extract; said step I′) is: I′) steam distilling dried rhizomes of Zingiber officinale , and concentrating the resulting distillate by evaporation to obtain the crude extract; and said step I″) is: I″) extracting powder of dried rhizomes of Zingiber officinale with supercritical CO 2 , recovering the resulting extraction solution of the supercritical CO 2 , and evaporating CO 2 from the extraction solution to obtain the crude extract, and wherein said first concentrated eluate is free of both 6-gingerol and 6-shogaol.