Patent ID: 8758989

Claim:
A method for detecting an enzyme, or an inhibitor thereof, within a test sample, the method comprising: contacting a test sample with a plurality of substrate conjugates to form an incubation mixture that includes the substrate conjugates and the test sample, said substrate conjugates each comprising a substrate joined to a reporter, wherein an enzyme-catalyzed reaction of said substrate conjugate forms a product conjugate, said product conjugate comprising a product of the enzyme-catalyzed reaction joined to the reporter, said reporter being capable of directly or indirectly generating an optically detectable detection signal; applying said incubation mixture to a chromatographic medium, said chromatographic medium comprising a first detection zone within which said product conjugate preferentially binds, said chromatographic medium further comprising a second detection zone within which said substrate conjugate preferentially binds, said incubation mixture migrating through said chromatographic medium to reach said first detection zone and said second detection zone, wherein the binding of the product conjugate generates a first detection signal within said first detection zone, and wherein the binding of the substrate conjugate generates a second detection signal within said second detection zone; and optically determining the presence or intensity of said first detection signal within said first detection zone and said second detection signal within said second detection zone.