Patent ID: 8101345

Claim:
A method for identifying a modified antisense oligonucleotide that is not proinflammatory via a TREM-2 mediated pathway comprising: contacting a cell with the modified antisense oligonucleotide wherein: the cell functionally expresses a TREM-2 extracellular domain, an intracellular signaling domain, and a reporter construct comprising a cis-acting DNA consensus element sensitive to activation of the intracellular domain, and wherein the cis-acting DNA consensus element is functionally linked to a reporter gene coding sequence; and wherein the modified antisense oligonucleotide does not comprise a CpG motif, and wherein said modified antisense oligonucleotide specifically hybridizes to a target sequence other than TREM-2; assaying for activation of the reporter gene; and identifying the modified antisense oligonucleotide as not being proinflammatory via a TREM-2 mediated pathway when said reporter gene is not activated.