Patent ID: 8551755

Claim:
An isolated glucoamylase variant comprising a catalytic domain and a starch binding domain (SBD), said SBD comprising one or more amino acid substitutions at a position corresponding to position: 494, 495, 501, 502, 503, 508, 511, 517, 518, 519, 520, 525, 527, 531, 533, 535, 536, 537, 538, 539, 540, 545, 546, 547, 549, 551, 561, 563, 567, 569, 577, 579, and 583 of SEQ ID NO: 2 or corresponding to an equivalent position in a parent glucoamylase; wherein the equivalent position in a parent glucoamylase is determined by sequence identity and said parent glucoamylase has at least 80% amino acid sequence identity and less than 100% amino acid sequence identity with SEQ ID NO:2 and wherein the parent glucoamylase comprises the sequence of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, or 9.