Patent ID: 8247206

Claim:
A recombinantly produced chimeric fusion protein consisting of: (1) one or two fusion modules, each fusion module consisting of: a) a polypeptide sequence consisting of an insert-in-flap (IF) domain of an Escherichia coli SlyD chaperone or an Escherichia coli SlpA chaperone, b) a polypeptide sequence consisting of a subsequence of SEQ ID NO: 3, the subsequence starting with any amino acid located between amino acid number 1 to 20 of SEQ ID NO: 3 and ending with any amino acid located between amino acid number 70 to 89 of SEQ ID NO: 3, wherein the polypeptide sequence is fused to the N-terminal amino acid of the polypeptide sequence a), and c) a polypeptide sequence consisting of a subsequence of SEQ ID NO: 3, the subsequence starting with any amino acid located between amino acid number 90 to 97 of SEQ ID NO: 3 and ending with any amino acid located between amino acid number 103 to 107 of SEQ ID NO: 3, wherein the polypeptide sequence is fused to the C-terminal amino acid of the polypeptide sequence a); and (2) optionally a target polypeptide located at the N-terminal end, at the C-terminal end, or at the N-terminal and the C-terminal ends of the one or two fusion modules; and (3) optionally a hexa-histidine sequence tag consisting of the amino acid sequence of SEQ ID NO: 24 for facilitating purification and refolding of the recombinantly produced chimeric fusion protein; wherein the one or two fusion modules and the target polypeptide are optionally separated by a linker sequence and wherein the target polypeptide and the hexa-histidine sequence tag are optionally separated by a spacer sequence, and wherein the recombinantly produced chimeric fusion protein has protein folding activity.