Patent ID: 7396647

Claim:
A process for cleaving an analyte or a library of analytes at selected sites or sequences, said process comprising the steps of: (a) providing: (i) said analyte or library of analytes; (ii) a chimeric nucleic acid construct comprising at least a first segment comprised of four or more universal bases, and a second segment wherein the 5′ end of said first segment is attached to the 3′ end of said second segment; (iii) a restriction endonuclease; (iv) reagents for hybridization and endonucleolytic digestion; (b) hybridizing said composition (ii) with said analyte or library of analytes (i) to form hybrids between said second segment and said selected sites or sequences in said analyte or library of analyte (i); and (c) cleaving endonucleolytically said hybrids.