Patent ID: 8831893

Claim:
Method for estimation of kinetic characteristics for the formation of a complex AB by interaction of reactants A and B, comprising the steps of: a) providing a lateral flow substrate matrix, wherein the substrate matrix comprises at least a first spot and a second spot having immobilized reactant B, wherein the second spot is positioned in a direction downstream of the first spot; b) causing a fluid sample with an amount of reactant A to flow through the substrate matrix so that the sample is sequentially contacted with the first spot and the second spot and the complex AB is formed at each of the first spot and the second spot; c) measuring a first signal related to the amount of reactant A captured by the immobilized reactant B in the complex AB on the first spot; d) measuring a second signal related to the amount of reactant A captured by the immobilized reactant B in the complex AB on the second spot; e) estimating the ratio of the first signal to the second signal; and f) based on the ratio obtained in step e) estimating kinetic characteristics comprising the formation rate constant (kon) and/or the dissociation rate constant (koff) for the formation and/or dissociation, respectively, of the complex AB.