Patent ID: 8350013

Claim:
A method for reducing turbidity and impurities in an eluate comprising a product, the method comprising: (a) providing a load fluid comprising a product and one or more impurities, wherein the product is an Fc-containing monoclonal antibody; (b) contacting the load fluid with a medium, wherein the medium is a Protein A chromatography column, and wherein the medium can bind the product under conditions suitable for binding the product, thereby providing a bound medium; (c) contacting the bound medium with one or more wash solutions, wherein at least one first wash solution comprises arginine in a concentration of about 0.5 M to about 1.0 M, wherein the pH of the wash solution is greater than 5.0 and less than about 8.0, thereby providing a washed medium; (d) contacting the bound medium with a second wash solution; (e) contacting the washed medium with an elution solution under conditions suitable for eluting the product, and (f) collecting an eluate comprising the product, wherein the ratio of the product to host cell protein in the eluate is increased and the eluate has reduced turbidity compared to an eluate recovered in a corresponding method in which no detectable amount of arginine is used in the one or more wash solutions in step (c).