Patent ID: 7829664

Claim:
An isolated polynucleotide whose nucleotide sequence is shown in SEQ ID NO:20 and is modified from SEQ ID NO:6 in that one or more codons of SEQ ID NO:6 encoding the amino acid sequence LLATMG is replaced with a codon selected from the group consisting of GCA/C/G/T, AAC/T, GAC/T, TGC/T, GAC/T, GAA/G, TTC/T, GGA/C/G/T, CAC/T, ATA/C/T, AAA/G, CTA/C/G/T, TTA/G, ATG, AAC/T, CCA/C/G/T, CAA/G, AGA/G, CGA/C/G/T, AGC/T, TCA/C/G/T, ACA/C/G/T, GTA/C/G/T, TGG, TAC/T, CAA/G and GAA/G, wherein said modified, isolated polynucleotide encodes a human pro-insulin leader, a glucagon-like peptide-1 (GLP-1), and a furin cleavable site between the human pro-insulin leader and the GLP-1.