Patent ID: 8840776

Claim:
A method for estimation of concentration of an analyte in a sample, comprising: a) bringing the sample into contact with a sensor strip, the sensor strip including a reference electrode coated with a reference layer, and a working electrode coated with a working layer, and being configured such that the sample flows from the reference electrode to the working electrode, the reference layer being nonreactive to the analyte, the working layer including a mediator for enhancement of electron transfer, and a biorecognition element reactive to the analyte; b) applying a first voltage between the reference electrode and the working electrode, such that an electrical potential at the reference electrode is higher than that at the working electrode, and recording first information of a reaction current flowing through the sample as a result of application of the first voltage within a time period of not greater than 5 seconds from beginning of application of the first voltage, wherein the first information is related to hematocrit of the sample and is unrelated to the analyte in the sample; c) estimating the hematocrit of the sample by analyzing the recorded first information using a predetermined current-hematocrit function; d) applying a second voltage between the reference electrode and the working electrode, such that an electrical potential at the reference electrode is lower than that at the working electrode, and recording second information of a reaction current flowing through the sample as a result of application of the second voltage, wherein the second information is related to the concentration of the analyte in the sample; and e) estimating the concentration of the analyte in the sample based on the hematocrit of the sample estimated in step c) and the second information recorded in step d).