Patent ID: 8043635

Claim:
A method for inhibiting inflammatory activity in skin tissue of a mammal, said method comprising: providing a bioactive composition comprising an isolated bioactive fraction derived from cell juice of a Camellia sinensis plant and applying the bioactive composition to the skin tissue in an amount effective to inhibit inflammatory activity in the skin tissue, wherein said isolated bioactive fraction is produced by a process comprising: (i) providing fresh biomass of a Camellia sinensis plant, wherein said fresh biomass comprises fresh Camellia sinensis plant biomass that has not undergone conventional tea processing; (ii) separating the fresh biomass of the Camellia sinensis plant into cell juice and a cell walls component, wherein said separating comprises grinding, macerating, and pressing the fresh biomass to separate the fresh biomass into the cell juice and the cell walls component; (iii) treating the cell juice under conditions effective to yield a bioactive fraction, said conditions comprising subjecting the cell juice to microwave treatment to induce coagulation, thereby yielding a coagulated membrane fraction and a non-coagulated portion of the cell juice, wherein said bioactive fraction is the coagulated membrane fraction; and (iv) isolating said bioactive fraction from the treated cell juice.