Patent ID: 7879546

Claim:
A method of diagnosing risk of HPV 18-induced neoplasia comprising the steps of: quantifying a first level of one or more HPV 18 mRNA from a sample collected from a patient infected with HPV, wherein the one or more HPV 18 mRNA is selected from the group consisting of E2 mRNA, E4 mRNA, E6 mRNA, and E7 mRNA; quantifying a second level of one or more HPV 18 mRNA from a sample collected from said patient, wherein the one or more HPV 18 mRNA is selected from the group consisting of E2 mRNA, L1 mRNA, and L2 mRNA; and determining a ratio of the quantified first and second levels of HPV 18 mRNA, wherein the ratio is selected from the group consisting of (E6+E7)/L1, (E6+E7)/(L1+L2), (E2+E4+E6+E7)/(L1+L2), and (E6+E7)/E2, wherein a ratio of greater than 2 is indicative of HPV 18-induced cell transformation and risk of neoplasia.