Patent ID: 7432108

Claim:
An apparatus for analyzing a plurality of amino acids in a fluid sample by a user comprising: a container for supplying a buffer solution including a lithium ion; a control valve for controlling a lithium ion concentration of said buffer solution, wherein the lithium ion concentration of said buffer solution is controlled by changing the mixture ratio of a plurality of solutions by controlling the time said control valve is open and closed; an auto sampler for supplying said fluid sample; a single separation column for separating said plurality amino acids in said buffer fluid samples; a detector for detecting concentration of said buffer solution; a processor in communication with said control valve and said auto sampler; and an analysis program residing on said processor for causing said processor to perform the acts of: introducing said sample into said buffer solution; passing said sample in said buffer solution through a separation column; increasing the lithium ion concentration until Hylys elutes; fixing a lithium ion concentration of said buffer solution from a time Hylys elutes to a time His elutes; and displaying said analysis for said user.