Patent ID: 8557748

Claim:
An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method comprising: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a treatment for binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.: wherein, in Formula (I), R 1 represents a trivalent linear or branched aliphatic group or aromatic group; R 2 represents a substituted or unsubstituted C 1 to C 20 alkylene group, a substituted or unsubstituted C 1 to C 20 arylene group, a substituted or unsubstituted C 1 to C 20 arylalkylene group or a substituted or unsubstituted C 1 to C 20 alkylarylene group; R 3 represents a hydrogen atom or a C 1 to C 10 alkyl group; X represents an alkoxy group (—OR 4 ), a halogen atom or an acyloxy group (—OOCR 5 ), wherein R 4 and R 5 each independently represent a hydrogen atom or a C 1 to C 10 alkyl group; n represents 1, 2 or 3; and m represents an integer of (3−n).