Patent ID: 8575326

Claim:
A method for detecting anogenital cancer or preliminary stages thereof in a sample, comprising determining the methylation status of ASTN1 (astrotactin 1) and ZNF671 (zinc finger protein, transcription factor), wherein ASTN1 and/or ZNF671 are methylated in a positive sample, and wherein primer pairs are used which comprise the following sequences: (a) CGTAAGCGTTGTTAGCGTAGC (ASTN1_F) (SEQ ID No.: 1) and CGCGAAATCGAAACGAAAACG (ASTN1_R); (SEQ ID No.: 2) (b) CGGAGGACGTAGTATTTATTCGC (ZNF671_F) (SEQ ID No.: 11) and CTACGTCCCCGATCGAAACG (ZNF671_R). (SEQ ID No.: 12)