Patent ID: 8808648

Claim:
A disposable device for holding and processing a sample for use in an apparatus for analyzing a liquid sample by nucleic acid amplification, comprising: a device body having: (1) a sheet comprising a flat plane, the sheet comprising: (i) a back side comprising a plurality of back side ribs disposed on the back side of the sheet, and (ii) a front side on which a structured surface is arranged, the structured surface comprising: a plurality of unitary front side ribs configured to increase stiffness of the device body, the front side ribs comprising a width which is about 30% to about 300% of a thickness of the sheet and a height which is about 60% to about 200% of the thickness of the sheet, the front side ribs comprising: flat tops, the plurality of front side ribs disposed on the front side of the sheet, (2) a sealing cover comprising a thickness of less than 1mm, the sealing cover attached to the flat tops of the front side ribs and covering the structured surface of the front side of the sheet, together comprising: (i) one or more chambers one of which comprises a sample preparation chamber having an elongated concave body recessed into the front side of the sheet and comprising a cylindrical opening situated below the plane of the sheet, the cylindrical opening configured to receive a sample transfer tip, (ii) one or more channels one of which comprises an inlet channel connected to an amplification chamber for providing the amplification chamber with liquid, and (iii) one or more air pockets between the front side of the sheet and the sealing cover, the air pockets thermally insulating the front side of the sheet from the sealing cover.