Patent ID: 7932502

Claim:
A fluorescence observation apparatus comprising: a light source section that emits excitation light for exciting a first fluorescent substance and a second fluorescent substance; a fluorescence detection section that detects first fluorescent light emitted when the first fluorescent substance is excited by the excitation light and second fluorescent light emitted when the second fluorescent substance is excited by the excitation light; a correction value calculation section that calculates a correction value for canceling a color mixture between the first fluorescent light and the second fluorescent light based on feature values corresponding to characteristics of the first fluorescent substance and the second fluorescent substance; a detected image generation section that generates a first detected image corresponding to a detection result of the first fluorescent light in the fluorescence detection section and a second detected image corresponding to a detection result of the second fluorescent light in the fluorescence detection section; and an image correction section that corrects a luminance value of each pixel of the first detected image to a luminance value corresponding to intensity of the first fluorescent light and corrects a luminance value of each pixel of the second detected image to a luminance value corresponding to intensity of the second fluorescent light based on the correction value.