Patent ID: 7659102

Claim:
A composition comprising: (A) a polypeptide, wherein the polypeptide has an alpha-amylase activity and: (a) the polypeptide is encoded by a nucleic acid comprising: (i) a sequence having at least 94% sequence identity to SEQ ID NO:1, (ii) a fragment of (i) encoding an enzymatically active fragment; (iii) the nucleic acid of (i), wherein the nucleic acid sequence comprises the sequence of SEQ ID NO:1 or a polypeptide having the sequence of SEQ ID NO:2; (iv) a nucleic acid comprising a sequence that hybridizes under stringent conditions to the nucleotide sequence of SEQ ID NO:1, wherein the nucleic acid encodes a polypeptide having an alpha-amylase activity and the stringent conditions include a wash step comprising a wash in 0.2×SSC at a temperature of about 65° C. for about 15 minutes, and the nucleic acid has at least 94% sequence identity to SEQ ID NO:1; or (v) the nucleic acid of any of (i) to (iv), wherein the nucleic acid sequence is codon-optimized for increased expression in a host cell, wherein the codon optimization comprises replacing a codon with a different codon encoding the same amino acid as the replaced codon; or (b) the polypeptide comprises (i) an amino acid sequence having at least 94% sequence identity to SEQ ID NO:2, (ii) an enzymatically active fragment of (i); or (iii) the amino acid sequence of SEQ ID NO:2; or (B) a glycosylated version of the polypeptide of(A).