Patent ID: 8105797

Claim:
A method of increasing the sensitivity of a diagnostic test for diagnosing Mycobacterium tuberculosis infection in a human, wherein said diagnostic test comprises contacting T cells from said human with a Mycobacterium tuberculosis antigen which is not Rv3879c, and determining whether any of the said T-cells recognise said antigen; said method additionally comprising (i) contacting T-cells from said human with one or more of (a) a peptide consisting of the sequence of SEQ ID NO: 1; (b) a peptide consisting of the sequence of at least 8 consecutive amino acids of the sequence shown in SEQ ID NO: 1; or (c) a peptide consisting of a sequence which is capable of binding to a T-cell receptor which recognises a peptide as defined in (a) or (b); and (ii) determining whether any of the said T-cells recognise said peptide, wherein the method is optionally carried out in vitro.