Patent ID: 7304208

Claim:
A method of producing human serum albumin (HSA) in monocot plant seeds, comprising the steps of: (a) transforming a monocot plant cell with a chimeric gene comprising (i) a promoter from the gene of a maturation-specific monocot plant storage protein, wherein the promoter comprises SEQ ID NO.:8, (ii) a first DNA sequence, operably linked to the promoter, encoding a signal sequence, and (iii) a second DNA sequence, linked in translation frame with the first DNA sequence, encoding HSA, wherein the first DNA sequence and the second DNA sequence together encode a fusion protein comprising an N-terminal signal sequence and the HSA; (b) growing a monocot plant from the transformed monocot plant cell for a time sufficient to produce seeds containing the HSA; and (c) harvesting the seeds from the plant, wherein the HSA constitutes at least about 10% of the total soluble protein in the harvested seeds.