Patent ID: 6942976

Claim:
A method of the detection and identification of Anacardium species specific sequences from a sample, said method comprising the steps of: (i) amplifying 5S rRNA non-coding spacer region from Anacardium occidentale by polymerase chain reaction with a pair of primers, wherein said primers are a consensus forward primer consisting of SEQ ID NO: 1 and a reverse primer consisting of SEQ ID NO: 2, (ii) analyzing the PCR product by agarose gel electrophoresis wherein said analyzing comprises identifying, excising and purifying the PCR product from the agarose gel, (iii) cloning the purified band into an appropriate vector and transforming the vector into E. coli cells, (iv) analyzing the clones for the presence of the insert, and selecting a clone having the insert, (v) sequencing the selected clone using a T7 promoter primer consisting of SEQ ID NO: 3 and M-13 forward primer consisting of SEQ ID NO: 4, and (vi) analyzing the sequence by comparing it with the spacer sequences of bamboo and tea, and designing an Anacardium occidentale specific primer having 28 base pairs, wherein said primer consists of SEQ ID NO: 5.