Patent ID: 8318172

Claim:
A method of producing or enhancing an immunological response in a subject against a target moiety, wherein the target moiety is TNFα, the method comprising: providing an unnatural immunogen, which unnatural immunogen comprises one or more unnatural amino acids, wherein the unnatural immunogen is an unnatural mTNFα, and the one or more unnatural amino acids is or are selected from the group consisting of: a pNO 2 Phe 11 -mTNFα, a pNO 2 Phe 19 -mTNFα, a pNO 2 Phe 21 -mTNFα, a pNO 2 Phe 42 -mTNFα, a pNO 2 Phe 49 -mTNFα, a pNO 2 Phe 86 -mTNFα, a pNO 2 Phe 104 -mTNFα, a pNO 2 Phe 113 -mTNFα; or wherein the unnatural immunogen is an unnatural hTNFα, and the one or more unnatural amino acids is or are selected from the group consisting of: a pNO 2 Phe 11 -hTNFα, a pNO 2 Phe 19 -hTNFα, a pNO 2 Phe 21 -hTNFα, a pNO 2 Phe 42 -hTNFα, a pNO 2 Phe 49 -hTNFα, a pNO 2 Phe 87 -hTNFα, a pNO 2 Phe 105 -hTNFα, and a pNO 2 Phe 114 -hTNFα; wherein the pNO 2 Phe unnatural amino acid residues are numbered relative to the mature mTNFα and hTNFα polypeptides; and, administering the unnatural immunogen to the subject, wherein the subject produces one or more antibodies against the unnatural immunogen, which antibodies are cross-reactive against TFNα; thereby producing or enhancing the immunological response against TFNα.