Patent ID: 8802431

Claim:
An isolated population of basement cells, wherein said basement cells are phase-dark as evidenced by light microscopy and have all of the following seven characteristics: i) the cells exhibit protein and/or gene expression of brachury, ii) the cells exhibit protein and/or gene expression of alpha-sarcomeric actin, iii) the cells exhibit no protein expression of vimentin, iv) the cells exhibit no protein expression of desmin, v) the cells exhibit no antigen expression of SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, and Oct-4, vi) the cells exhibit gene expression of Basonuclin 1 (BNC1), nephronectin (NPNT), fibrinogen alpha chain (FGA), annexin A8 (ANXA8), matrix Gla protein (MGP), zona pellucida glycoprotein 4 (ZP4), thrombomodulin (THBD), homeobox B7 (HOXB7), heart and neural crest derivatives expressed 1 (HAND1), desmocollin 3 (DSC3), interleukin 6 signal transducer (IL6ST), aquaporin 1 (AQP1), Frizzled-related protein (FRZB), bradykinin receptor B1 (BDKRB1), growth hormone receptor (GHR) and kinase insert domain receptor (Flk-1) (KDR), and vii) the cells have down regulated gene expression of oct-4 and nanog compared to undifferentiated hBS cells.