Patent ID: 8114611

Claim:
A method or purifying a target molecule from a mixture comprising: (1) contacting the mixture comprising the target molecule with an affinity chromatography matrix under a set of pH and/or salt conditions, wherein the affinity chromatography matrix comprises: a) a solid support; b) a protein ligand bound to the solid support, wherein the protein ligand binds to the target molecule under the set of pH and/or salt conditions; c) an activating group bound to the solid support which interacts covalently with the protein ligand: and d) an associative group bound to the solid support, where the associative group interacts non-covalently with the protein ligand and wherein the activating group and the associative group are both linked separately to the solid support: and (2) altering the set of pH and/or salt conditions of (1) such that the target molecule no longer binds to the protein ligand of the matrix, thereby purifying the substance of interest.