Patent ID: 6936427

Claim:
A method to determine whether a first substance interacts with a second substance or to determine the affinity of the first substance for a second substance which method comprises contacting said first substance coupled to a first demitope with said second substance coupled to a second demitope complementary to the first demitope, wherein said substances are heterologous to the demitopes, and their interaction and affinities are not known, in the presence of a reporter, wherein said first and second demitopes, when assembled, form a paratope which binds said reporter, and wherein said reporter generates an immediate detectable signal when bound to the paratope, and determining the presence, absence or magnitude of the signal produced; wherein the presence of the signal indicates said first and second substances interact and the absence of signal indicates the first and second substance do not interact, or the magnitude of the signal is a measure of the affinity of interaction of said first substance and said second substance.