Patent ID: 8605971

Claim:
A method for identifying an optimal antibody titer for an immunoassay comprising: receiving pixelized images of a plurality of tissue sample sets, each tissue sample set comprising a plurality of different tissue samples each harboring a concentration of an analyte and each stained with a respective antibody titer dilution for detection of the analyte concentration, different tissue sample sets having different respective antibody titer dilutions; performing a quantitative analysis of the pixelized images of the plurality of tissue sample sets, resulting in a respective selectivity value indicative of a staining intensity of each of the different tissue samples of each of the plurality of tissue sample sets; determining from pixel intensities of the pixelized images of each of the different tissue samples of each of the plurality of tissue sample sets, a respective sensitivity value, and for each tissue sample set of the plurality of tissue sample sets, a dynamic range for the analyte concentration; and identifying from the respective selectivity values and the respective sensitivity values of each of the different tissue samples of each of the plurality of tissue sample sets, an optimal antibody titer dilution that provides for an optimal dynamic range across all of the different tissue samples.