Patent ID: 8058008

Claim:
A method for screening a library of compounds to detect a biologically active compound by detecting intracellular translocation of a subunit of a component of an intracellular pathway affecting intracellular processes, which subunit exhibits a biological activity of the component, comprising: (a) culturing one or more cells containing a nucleotide sequence coding for a hybrid polypeptide comprising a luminophore linked to the subunit under conditions permitting expression of the nucleotide sequence, (b) incubating the one or more cells with at least one compound of the library of compounds, (c) screening the library of compounds to determine whether the at least one compound of the library of compounds has a biological function or biological effect on the subunit in the one or more cells, wherein translocation of the subunit in response to the at least one compound of the library of compounds determines that the at least one compound has a biological function or biological effect on the subunit, and (d) measuring the light emitted from the luminophore in the incubated one or more cells and determining a variation with respect to the emitted light from said luminophore, such variation being indicative of the translocation of the subunit in said one or more cells and said translocation being indicative that said at least one compound of the library of compounds to be screened is biologically active with the component.