Patent ID: 7601536

Claim:
A vector, comprising: (1) a first polynucleotide, comprising the consensus sequence [SEQ ID NO:84][A/C/G]-[A/C/T]-[A/C/T]-[G/T]-A-[C/G]-NNNNNN-A-[G/T]-A-[A/C/T]-[A/G]-TCCTG-[C/G/T]-[A/C/G]-N (SEQ ID NO:84), which is (i) nicked when exposed to an enzyme involved in bacterial-mediated plant transformation and (ii) not identical to a bacterial border sequence; (2) a second polynucleotide, which is a bacterial T-DNA border; and (3) a desired polynucleotide positioned between the first and second polynucleotides.