Patent ID: 8361782

Claim:
A method of assaying in a microfluidic device the amount of an analyte in a biological fluid, said microfluidic device having at least one sample inlet port, at least one air vent, and at least one reagent-containing chamber, comprising: (a) dispensing a sample of said biological fluid into said at least one sample inlet port of said microfluidic device, said sample moving by capillary force to a capillary stop through a capillary passageway communicating with said at least one sample inlet; (b) dispensing into said at least one inlet port of (a) a portion of a liquid different from said sample of (a), said liquid portion being sufficient to force said sample past said capillary stop, said liquid portion being dispensed in the form of a group of droplets having diameters in the range of 0.05 to 1 mm, said groups of droplets being separated by intervals when no droplets are dispensed, said liquid portion being added at a predetermined time after introducing said sample.