Patent ID: 7659060

Claim:
A method for identifying a nucleotide polymorphism, the method comprising: (a) hybridizing a labeled oligonucleotide to a nucleic acid containing a specific nucleotide polymorphic site in a sample, wherein the oligonucleotide has a sequence complementary to a wild type or mutant sequence at the specific nucleotide polymorphic site in the nucleic acid, wherein the label of the oligonucleotide is Texas Red™ (1H, 5H, 11H, 15H-xantheno[2,3,4-ij:5,6,7-i′j′]diquinolizin-18-ium, 9-(2-sulfo-4-chlorosulfonphenyl)-2,3,6,7,12,13,16,17-octahydro-, inner salt), and wherein the Texas Red™ is attached to the 3′ end of the oligonucleotide; (b) allowing a nucleic acid-specific label to act on a mixture of the oligonucleotide and the nucleic acid, wherein the nucleic acid-specific label is SYBR™ Green I; (c) detecting an interaction between the label of the oligonucleotide and the nucleic acid-specific label, wherein the interaction is fluorescence resonance energy transfer; and (d) identifying a nucleotide polymorphism, wherein the identification utilizes a difference due to the nucleotide polymorphism in conditions under which the hybridized oligonucleotide is separated into a single strand, and wherein the identification can distinguish a nucleic acid containing a specific nucleotide polymorphism derived from a heterozygote from a nucleic acid containing a specific nucleotide polymorphism derived from a homozygote.