Patent ID: 8865445

Claim:
A DNA construct or recombinant expression vector comprising an isolated polynucleotide comprising a nucleotide sequence that encodes a GH61 polypeptide having cellulolytic enhancing activity, wherein the isolated polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of: (a) a GH61 polypeptide having at least 95% sequence identity to amino acids 20 to 334 of SEQ ID NO: 4; (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 58 to 1250 of SEQ ID NO: 3, (ii) the cDNA sequence of nucleotides 58 to 1250 of SEQ ID NO: 3, or (iii) the full-length complement of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.; the cDNA sequence of nucleotides 58 to 1250 of SEQ ID NO: 3; (c) a GH61 polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 58 to 1250 of SEQ ID NO: 3, or a GH61 polypeptide encoded by the cDNA of nucleotides 58 to 1250 of SEQ ID NO: 3; and (d) an isolated fragment of a GH61 polypeptide of (a), (b), or (c) that has cellulolytic enhancing activity.