Patent ID: 8754014

Claim:
A method for selecting an oocyte, that when fertilized produces a viable embryo with a high implantation rate leading to pregnancy, comprising the steps of measuring the expression level of 45 genes, using a technique selected from the group consisting of hybridization, amplification, electrophoresis, immunoassay, and immunohistochemical assay, in a cumulus cell surrounding said oocyte, wherein said genes are WNT6, LRCH4, PAX8, CABP4, PDE5A, BCL2L11, PCK1, TCF20, SLAMF6, EPOR, CACNG6, NLRP1, PECAM1, NOS1, ATF3, KRTAP8, GRIK5, SLC24A3, SLC5A12, SLCA10A2, SLCO1A2, SLC25A5, MG29, NLGN2, PRKACA, FOSB, SIAT6, LOXL2, PRF1, ADPRH, APBB3, EGR3, CNR2, IFITM1, PLA2G5, CAMTA1, SOX4, NFIB, NFIC, RBMS1, GOS2, FAT3, SLC40A1, GPC6 and IGF1R, and comparing said expression levels of said genes in said cumulus cell to reference values obtained from a control, wherein the control is a sample comprising cumulus cells associated with an oocyte, wherein detecting differences in expression levels of said genes in said cumulus cell and said control is predictive of implantation rate, and selecting an oocyte for fertilization and implantation based on a positive prediction of said high implantation rate.