Patent ID: 8772234

Claim:
A pharmaceutical preparation comprising: a) a polypeptide comprising a bone morphogenetic protein-10 (BMP10) propeptide; and b) a pharmaceutically acceptable carrier, wherein the BMP10 propeptide comprises an amino acid sequence at least 98% identical to the amino acid sequence of SEQ ID NO: 1, wherein the BMP10 propeptide contains no more than 20 contiguous amino acids of a mature portion of a BMP10 polypeptide and comprises an alteration in the amino acid sequence that alters glycosylation of the BMP10 propeptide relative to the naturally occurring BMP10 propeptide when produced in a mammalian cell, and wherein said BMP10 propeptide has one or more of the following characteristics: i) binds to mature BMP10 with a Kd of at least 10 −5 M; and ii) inhibits BMP10 signaling in a cell.