Patent ID: 7807390

Claim:
A method for identifying an agonist or antagonist of a human SP9155 receptor that modulates the ability of a polypeptide ligand comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4-11 and 13-18, wherein the carboxy-terminus of said polypeptide ligand is amidated, to bind to said receptor, comprising: (a) contacting the SP9155 receptor or a ligand-binding fragment thereof, which receptor or fragment thereof is capable of binding a polypeptide ligand comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 4-11 and 13-18, wherein the carboxy-terminus of said polypeptide ligand is amidated, in the presence of a known amount of labeled polypeptide ligand for the SP9155 receptor or ligand-binding fragment thereof, wherein said polypeptide ligand comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 4-11 and 13-18, wherein the carboxy-terminus of said polypeptide ligand is amidated, with a sample to be tested for the presence of said agonist or antagonist; and (b) measuring the amount of the ligand specifically bound to the receptor; whereby the sample is identified as containing said antagonist or an agonist by measuring substantially reduced binding of the labeled ligand to the receptor, compared to what would be measured in the absence of the sample.