Patent ID: 6982087

Claim:
A South African Arbovirus No. 86 (S.A.AR86) genomic RNA, said S.A.AR86 genomic RNA comprising: (a) S.A.AR86 nonstructural protein (nsp) and/or S.A.AR86 structural protein coding sequences, wherein the S.A.AR86 nonstructural protein and/or structural protein coding sequences encode an attenuating mutation selected from the group consisting of: (i) an attenuating mutation in the cleavage domain between the nsp1 and nsp2 coding sequences; (ii) an attenuating mutation that results in a termination codon at nsp3 amino acid position 537, (iii) an attenuating mutation comprising a substitution mutation at nsp3 amino acid position 385, (iv) an attenuating mutation comprising an insertion of at least 8 amino acids following nsp3 amino acid position 385, (v) an attenuating mutation comprising a substitution at E2 amino acid position 243, and (vi) a combination of the attenuating mutations of (i) to (v) (b) a heterologous nucleotide sequence; and (c) an alphavirus capsid enhancer sequence operatively associated with said heterologous nucleotide sequence.