Patent ID: 8383410

Claim:
A method for in vitro detection of the risk of humoral transplant rejection in a patient who has received an organ transplant, said risk being linked to an increase in the presence of complement component C4d deposited on erythrocytes contained in a blood sample taken from the patient in comparison to a value obtained on the blood sample of said patient prior to transplantation or on a normal sample, comprising (i) quantitative determination of a complement component C4d deposited on erythrocytes contained in a blood sample taken from the patient, by reference to the value measured on an internal standard, wherein the internal standard consists of a support of micro-particles or stabilized erythrocytes coated with a known quantity of complement component C4d in the form of C4d fragments or molecules containing it provided that the C4d binding site is accessible for detection, the known quantity consistent with that observed on erythrocytes of transplant patients with humoral transplant rejection, (ii) comparison of the quantitative determination in step (i) with a value obtained on the blood sample of said patient prior to transplantation or on a normal sample.