Patent ID: 8703427

Claim:
A compound comprising a plurality of photosensitizing agents coupled to a carrier molecule obtainable by the process of (i) providing a photosensitizing agent; (ii) providing a carrier molecule; (iii) conjugating the photosensitizing agent and the carrier molecule in the presence of a first and a second polar aprotic solvent and an aqueous buffer, wherein the carrier molecule is a human or humanized scFv wherein the photosensitising agents are directly coupled at an amino acid residue of the carrier molecule so as to not sterically, chemically or photophysically interfere with each other when participating in chemical or photochemical reactions and which are coupled 3.5 to 7.5 angstroms, or 9 to 12 angstroms or 10-15 angstroms or 15-20 angstroms or 20-25 angstroms apart; and wherein the ratio of photosensitizing agent to carrier molecules is at least 3:1.