Patent ID: 7759120

Claim:
A method comprising: inserting an implantable medical device having a lumen into a protective sleeve; inserting the protective sleeve into a tube through an open end of the tube, wherein the tube defines a longitudinal axis; sealing the open end of the tube with a self-sealable membrane fabricated from a self-sealable material, wherein the self-sealable membrane provides a selective liquid barrier at the open end of the tube; delivering a preparation media comprising calcium to said lumen of the implantable medical device by penetrating said self-sealable membrane with a delivery device; after a first application period, removing any residue preparation media from said lumen through said self-sealable membrane with a removal device; subsequent to removing any residue preparation media, delivering a blood centrifugation product comprising blood plasma to said lumen of the implantable medical device by penetrating said self-sealable membrane with the delivery device; after a second application period, removing any residue of the blood centrifugation product from said lumen through said self-sealable membrane with the removal device; subsequent to removing any residue centrifugation product, delivering cells to said lumen of the implantable medical device by penetrating said self-sealable membrane with the delivery device; withdrawing the delivery device to re-establish the liquid barrier at said self-sealable membrane; and centrifuging the implantable medical device with the cells in the lumen by rotating the tube substantially around the longitudinal axis.