Patent ID: 7741286

Claim:
A conjugate formed by a method comprising covalently coupling a modified kringle 5 peptide with human serum albumin, wherein the modified kringle 5 peptide comprises a kringle 5 peptide and a maleimido group coupled to the kringle 5 peptide, optionally via a linking group; wherein the kringle 5 peptide is a peptide fragment of amino acid residues 443 to 546 of SEQ ID NO: 1, of between 4 and 104 amino acids, and optionally having an amino acid substitution at Cys 523 ; wherein the maleimido group and optional linking group is optionally coupled to the kringle 5 peptide by one or more additional amino acids; wherein the kringle 5 peptide is optionally coupled to an N-terminal protecting group; wherein the kringle 5 peptide has an α-C-terminal carboxy, an α-C-terminal carboxamide, or an α-C-terminal alkylcarboxamide; wherein said maleimido group reacts with a thiol group of Cys 34 of said human serum albumin to form a conjugate that has a 1:1 loading of said kringle 5 peptide to said human serum albumin.