Patent ID: 8642282

Claim:
A method of reducing activity of a nitric oxide synthase expressed by a human melanocyte or melanoma cell, said method comprising providing a compound selected from compounds of a formula wherein R 1 is selected from H, halogen, alkyl, and substituted alkyl moieties; R 2 is selected from H, alkyl, aryl, substituted aryl and substituted alkyl moieties; Z is NH; and R 3 is selected from alkyl, substituted alkyl, arylalkylaminoalkyl, substituted arylalkylaminoalkyl, arylalkyl, substituted arylalkyl, aminoalkyl, and substituted aminoalkyl moieties, said substituents selected from halo and haloalkyl moieties, or salts thereof; and contacting at least one of a human melanocyte and a human melanoma cell expressing a nitric oxide synthase with an amount of said compound effective to reduce nitric oxide synthase activity, thereby reducing ultra-violet radiation induced nitric oxide production in a human melanocyte or melanoma cell.