Patent ID: 8369596

Claim:
A system for modifying image data from a biological probe array, wherein the system comprises: a computer, wherein the computer comprises a processor, wherein the processor is configured to access a non-transitory computer-readable storage medium, wherein the processor is configured to execute machine-readable instructions stored on the non-transitory computer-readable storage medium, and wherein the machine-readable instructions cause the processor to perform the steps of: (a) obtaining image data for a feature of a biological probe array, wherein the image data comprises a set of pixels; (b) determining an observed pixel intensity for each pixel in the set of pixels; (c) determining an initial feature intensity for the feature, wherein the initial feature intensity is based upon the observed pixel intensity; (d) determining a theoretical pixel intensity for each pixel in the set of pixels, wherein the theoretical pixel intensity is based, at least in part, upon the initial feature intensity, wherein determining the theoretical pixel intensity comprises determining an empirically based transfer function, and wherein determining the theoretical pixel intensity comprises multiplying the empirically based transfer function with the initial feature intensity; (e) determining an error for each pixel in the set of pixels, wherein the error is based upon the observed pixel intensity and the theoretical pixel intensity; and (f) determining an adjusted feature intensity, wherein the adjusted feature intensity is based upon the initial feature intensity and the error.