Patent ID: 8299217

Claim:
A recombinant polypeptide comprising a variant Cry3 amino acid sequence, wherein said variant comprises one or more amino acid substitutions in the amino acid sequence of SEQ ID NO:2, and wherein the one or more amino acid substitutions are selected from the group consisting of: a) an alanine, glutamine, or histidine residue at the position 154 of SEQ ID NO:2; b) a glutamic acid or a lysine residue at the position 155 of SEQ ID NO:2; c) a glycine residue at the position 158 of SEQ ID NO:2; d) an alanine, isoleucine, leucine, phenylalanine, threonine, or valine residue at the position 160 of SEQ ID NO:2; e) a methionine or tryptophan residue at the position 315 of SEQ ID NO:2; f) an alanine, glutamine, glutamic acid, or threonine residue at the position 316 of SEQ ID NO:2; g) an isoleucine or leucine residue at the position 482 of SEQ ID NO:2; h) a glutamine, lysine, or serine residue at the position 483 of SEQ ID NO:2; and i) an asparagine residue at the position 519 of SEQ ID NO:2; and wherein said variant has pesticidal activity.