Patent ID: 8133676

Claim:
A method of analysing genetic diversity of micro-organisms in a sample, wherein said sample comprises two or more member micro-organisms, said method comprising: a) isolating nucleic acid from the micro-organisms in said sample wherein the isolated nucleic acid comprises polymorphic gene sequences; b) providing at least two pairs of differently labelled primers, wherein a first primer pair with a first label is complementary to a first target sequence and at least a second primer with a second label is complementary to at least a second target sequence, and wherein said first and at least second target sequences are polymorphic gene sequences; c) amplifying the nucleic acid in a single multiplex PCR reaction; d) digesting the labelled amplified nucleic acid with a mixture of restriction enzymes to produce restriction fragments, and size sorting said fragments to produce a restriction fragment length profile; and e) analysing said restriction fragment length profile so obtained to genotype said two or more member micro-organisms, to allow analysis of the genetic diversity of the micro-organisms in the sample.