Patent ID: 7544658

Claim:
A heat resistant V 1 ATPase, which is a V 1 portion of a V 0 V 1 -ATPase derived from the thermophile bacteria, Thermus thermophilus , and is a complex molecule having three A subunits, three B subunits and one D subunit, wherein: the A and B subunits are arranged alternately to form a hexamer cylinder, and the D subunit is embedded in the central cavity of the cylinder, each A subunit has the amino acid sequence of SEQ ID NO: 3, each B subunit has the amino acid sequence of SEQ ID NO: 4 and the D subunit has the amino acid sequence of SEQ ID NO: 5, wherein, the A subunits have two substitutions: an Ala residue for the 232 nd Ser residue and Ser residue for the 235 th Thr residue in SEQ ID NO:3; wherein, optionally the D subunit further comprises a joint material, and wherein, the joint material is optionally bound to at least one Cys residue substituted for the 48 th Glu residue and Cys residue substituted for the 55 th Gln residue in SEQ ID NO: 5.