Patent ID: 7303906

Claim:
A method of preparing an Escherichia coli culture for transformation at increased efficiency as compared to E. coli K12 MG1655, the method comprising culturing the E. coli to an optical density at 650 nm of at least 0.4 in a medium, wherein the E. coli is an multi-deletion series strain lacking bases 263080-324632, 1398351-1480278, 2556711-2563500, 2754180-2789270, 2064327-2078613, 3451565-3467490, 2464565-2474198, 1625542-1650865, 4494243-4547279, 3108697-3134392, 1196360-1222299, 564278-585331, 15388-20563, 263080-324632, 564278-585331, 602639-608573, 1398351-1480278, 2064327-2078613, 2507650-2515969, 2556711-2563500, 2754180-2789270, 379293-387870, 389121-399029, 2992959-2996892, 3182796-3189712, 3451565-3467490, 2464565-2474198, 1625542-1650785, 4494243-4547279, 3108697-3134392, 1196360-1222299, 687074-688268, 1386912-1396646, 2099418-2135739, 2284410-2288200, 3359747-3365277, 3648921-3651343, 1128637-1140209, 1960605-1977294, 1995085-2021700, 4553059-4594581, 522062-529348, 728616-738185, 1525914-1531648, 3616623-3623309, 3759620-3767868, 1041253-1049768, 1085329-1096603, 2163172-2175230, 3578769-3582674, and 3718262-3719704 of the E. coli strain K12 MG1655.