Patent ID: 8592194

Claim:
An isolated glucoamylase variant comprising one or more amino acid substitutions at a position corresponding to residue position: 10, 14, 15, 23, 42, 45, 46, 59, 60, 61, 67, 68, 72, 73, 97, 98, 99, 102, 108, 110, 113, 114, 122, 124, 125, 133, 140, 144, 145, 147, 152, 153, 164, 175, 182, 204, 205, 214, 216, 219, 228, 229, 230, 231, 239, 240, 241, 244, 263, 264, 265, 268, 269, 276, 284, 291, 300, 301, 303, 310, 311, 313, 316, 338, 342, 344, 346, 349, 359, 361, 364, 379, 382, 390, 391, 393, 394, 408, 410, 415, 417, 418, 430, 431, 433, 436, 442, 443, 444, 448 and 451 of SEQ ID NO: 2 or SEQ ID NO: 3 or an equivalent position in a parent glucoamylase; wherein the equivalent position in a parent glucoamylase is determined by sequence identity and said parent glucoamylase has at least 80% amino acid sequence identity with the sequence of SEQ ID NO: 2, and wherein said parent glucoamylase comprises the sequence of SEQ ID NOs: 1, 4, 5, 6, 7, 8, or 9.