Patent ID: 7914983

Claim:
A method for human gene expression analysis, comprising: (i) generating oligonucleotide probes by a method comprising: (a) selecting a gene-specific target region sequence for each gene of interest for a signature set of genes the expression of which is indicative of a disease or condition; (b) synthesizing anti-sense oligonucleotide probes that complement each of said selected target sequence for each of said at least one known gene of interest; (c) mixing said oligonucleotide probes with isolated total RNA or mRNA to allow hybridization to occur; (d) adding a labeled dideoxy nucleotide to the 3′ end of hybridized oligonucleotide probes by polymerase reaction; and (e) recovering said oligonucleotide probes by removal or hydrolysis of said RNA; (ii) providing test oligonucleotides on a solid support, said test oligonucleotides being complements in sequence to the probe oligonucleotides, wherein said solid support is a microarray slide, and wherein said test oligonucleotides are arranged to form a microarray; (iii) hybridizing said probe oligonucleotides generated from step (i) above with said test oligonucleotides; and (iv) detecting and measuring signal intensity of said labels from said hybridized probe oligonucleotides on said solid support to determine the expression level of each of said at least one gene of interest wherein said signal intensity is proportional to the expression level of each gene.