Patent ID: 7241607

Claim:
A method for producing a fructosyl transferase: from Penicillium citrinum KCTC 10225BP microorganism that hydrolyzes sucrose into fructooligosaccharides of formula I and neofructooligosaccharides of formula II: wherein n is an integer of 1 to 5, G represents glucose and F represents fructose, which comprises: seed culturing in a first medium at 26° C. to 28° C. for 2 days while agitating at a speed of 100 to 200 rpm a Penicillium citrinum KCTC 10225BP microorganism; mass-producing said microorganism in a fermentation medium at 26° C. to 28° C. for 72 hours while agitating at a speed of 200 to 600 rpm and injecting air at a rate of 0.5 to 1 v/vm; and collecting the produced microorganisms by centrifugation, washing the collected microorganisms twice with 0.85% physiological saline, and culturing the washed microorganisms in a sucrose solution having a Brix concentration of 60 to 77 at a temperature of 35° C. to 50° C. and a pH of 5 to 7 for 20 to 50 hours at a speed of 100 to 300 rpm, wherein said fructosyl transferase is produced.