Patent ID: 6893865

Claim:
A method for producing a recombinant polynucleotide encapsidated in an adeno-associated virus (AAV) particle, comprising culturing a mammalian cell which produces AAV rep and cap gene products under conditions that are permissive for encapsidation of a recombinant polynucleotide into an AAV particle, wherein said mammalian cell contains the recombinant polynucleotide which comprises a heterologous gene operably linked to an encapsidation element which promotes encapsidation of said heterologous gene into an AAV particle, wherein said encapsidation element is other than an AAV inverted terminal repeat (ITR) or its sequence, and wherein said encapsidation element comprises the nucleotide sequence GGTTGG(X)nGCXCGCTCGCTCGCTX (SEQ ID NO:7), wherein X is any nucleotide and n is an integer from 1 to about 20; and wherein the heterologous gene is a foreign gene to AAV; whereby the recombinant polynucleotide encapsidated in the AAV particle is produced.