Patent ID: 8093004

Claim:
A method for detecting a single nucleotide polymorphism A46G in a β2AR gene in a human subject suffering from asthma and identifying the human subject as a good responder to a short acting β2 agonist based on the genotype at nucleotide position 46 of the beta 2AR gene said method comprising the steps of: (a) isolating genomic DNA from the human subject, (b) amplifying the genomic DNA using oligonucleotide PCR primers set forth in SEQ ID NOS. 2 and 3, (c) sequencing the PCR amplified genomic DNA obtained in step (b) (d) detecting the single nucleotide polymorphism A46G in the sequenced PCR amplified genomic DNA from step (c), (e) identifying specific β2AR genotypes AA, AG and GG, and (f) based on the information from step (e), identifying the human subject to be a good responder to the short acting β2 agonist if said subject has GG genotype and a poor responder to the short acting β2 agonist if said subject has the AA genotype.