Patent ID: 8178343

Claim:
A weighted gene cluster diagnosis apparatus, comprising a gene chip, said gene chip comprising a target gene cluster, said target gene cluster comprising a plurality of target genes related to a cancer, wherein said target gene cluster, an internal control and a blank control are obtained in arrays with each gene in triplicate on a nylon film to obtain a labeled specific sequence; a preprocessing unit, said preprocessing unit purifying mRNA obtained from a sample and labeling said mRNA to obtain probes after reverse-transcribing said mRNA into cDNA; a hybridizing region, said hybridizing region processing hybridization to said probes in said gene chip; a color development region, said color development region processing color development by applying diaminobenzidine (DAB) to said probes after said hybridization; and an analyzing unit, said analyzing unit having an analysis software, said analyzing unit having an image capturing mode and a data transferring mode, said analyzing unit having a procedure comprising steps of: capturing an image of said gene chip after said color development; processing an automatic analysis to said image of said gene chip; processing a gene weighted calculation to a plurality of detection values obtained after said automatic analysis; and multiplying a number of gene spots having positive reaction with weighted values for said gene spots to obtain a total score.