Patent ID: 7390391

Claim:
A method for measuring a concentration of a test target, the method comprising: constructing a reaction system by supplying the test target to a reagent layer containing an oxidation-reduction enzyme and an electron mediator; measuring the concentration of the test target by utilizing; wherein the test target is glucose, the oxidation-reduction enzyme is CyGDH, and a Ru compound is used as the electron mediator, the method further comprising: applying constant voltage to the reagent layer; and computing the concentration of the test target on the basis of a response current value from the reagent layer; wherein the constant voltage is no greater than 300 mV and selected from a range from a standard oxidation-reduction potential (versus a standard hydrogen electrode) between an oxidant and a reductant of the Ru compound through a standard oxidation-reduction potential (versus a standard hydrogen electrode) between ferrocyanide ions and ferricyanide ions.