Patent ID: 8143378

Claim:
A composition comprising a plurality of conjugates, each conjugate comprising one, two or three water-soluble polymers each covalently attached to a Factor VIII moiety polypeptide via a hydrolytically stable linkage, wherein: (i) the Factor VIII polypeptide is selected from the group consisting of Factor VIII, Factor VIIIa, Factor VIII:C, Factor VIII:vWF and B-domain deleted Factor VIII; (ii) the water-soluble polymer is selected from the group consisting of a poly(alkylene glycol), a poly(oxyethylated polyol), a poly(olefinic alcohol), a poly(vinylpyrrolidone), a poly(hydroxyalkylmethacrylamide), a poly(hydroxyalkylmethacrylate), a poly(saccharide), a poly(α-hydroxy acid), a poly(vinyl alcohol), a polyphosphazene, a polyoxazoline, a poly(N-acryloylmorpholine), and combinations thereof; and (iii) the composition is bioactive, comprising an in-vitro activity of at least 15% compared to that of a Factor VIII polypeptide composition in unconjugated form.