Patent ID: 8158367

Claim:
A cancer diagnostic method, comprising providing a sample containing antibodies from a subject suspected of having cancer, incubating the sample with Gb5 and one or more of Globo H, Bb2, Bb3, and Bb4 to allow binding of antibodies in the sample to the Gb5 and the one or more of Globo H, Bb2, Bb3, and Bb4, measuring both the amount of Gb5-bound antibodies and the amount of Globo H-bound, Bb2-bound, Bb3-bound, or Bb4-bound antibodies, and determining whether the subject has the cancer based on the amounts of all of the bound antibodies, wherein the cancer is both Globo H-positive and Gb5-positive; and a higher ratio of the amount of Globo H-bound, Bb2-bound, Bb3-bound, or Bb4-bound antibodies to the amount of Gb5-bound antibodies, as compared with that of a cancer-free subject, indicates that the subject has the cancer.