Patent ID: 8551710

Claim:
A method for localized in situ detection of at least one target RNA in a sample of one or more cells that was formalin-fixed paraffin-embedded (FFPE), comprising: generating a cDNA complementary to an RNA in the sample; adding a ribonuclease to said sample to digest the RNA hybridized to said cDNA; contacting said sample with one or more padlock probes wherein said padlock probe(s) comprise terminal regions complementary to said cDNA and hybridizing said padlock probe to the cDNA at said complementary terminal regions; circularising said padlock probe(s) by ligating, directly or indirectly, the ends of said padlock probe(s); subjecting said circularized padlock probe(s) to rolling circle amplification (RCA); detecting the rolling circle amplification product(s).