Patent ID: 8481294

Claim:
A mutant formate dehydrogenase, having improved durability and comprising any one of the following amino acid sequences: A) an amino acid sequence having a substitution of the 99 th valine with isoleucine, a substitution of the 153 rd valine with isoleucine, a substitution of the 155 th histidine with glutamine, and a substitution of the 298 th asparagine with tyrosine; B) an amino acid sequence having a substitution of 73 rd glycine with alanine, a substitution of the 99 th valine with isoleucine, a substitution of the 153 rd valine with isoleucine, and a substitution of the 155 th histidine with glutamine; and C) an amino acid sequence having a substitution of the 73 th glycine with alanine, a substitution of the 99 th valine with isoleucine, a substitution of the 153 rd valine with isoleucine, and a substitution of the 159 th glutamine with leucine; in the formate dehydrogenase consisting of the amino acid sequence shown in SEQ ID NO: 2.