Patent ID: 7335471

Claim:
A method for producing a RNA of interest, or proteins coded by this RNA of interest, comprising: synthesizing RNA with mutated RNA polymerases of phage origin, the peptide chain of which is modified, with respect to the wild-type RNA polymerases from which they originate, by substitution, or deletion, or addition of at least one amino acid, this modification having the effect of reducing the sensitivity of said RNA polymerases to the initial transcribed sequence (ITS) contained in a DNA sequence coding for an RNA of interest, starting from determined nucleotide sequences comprising a DNA sequence coding for said RNA of interest and the transcription of which is placed under the control of a promoter recognized by the abovementioned wild-type RNA polymerases and mutated RNA polymerases, said method having a production yield of said RNA greater than the yield obtained in the case of use of the wild-type RNA polymerases in the presence of the same non-consensual ITS as that contained in the DNA sequence coding for said RNA of interest, wherein said mutated RNA polymerases are selected from the group consisting of: those comprising the mutated T7 RNA polymerase represented by SEQ ID NO: 2, comprising a leucine in position 266 substituted for the proline in the wild-type T7 RNA polymerase, or of the proline situated in the homologous position in the wild-type RNA polymerases of other bacteriophages, selected from the group consisting of the proline situated in positions 267 in T3, 289 in KII, and 239 in SP6.