Patent ID: 8003409

Claim:
A fluorescence measurement method comprising: forming semiconductor nanoparticles from a material that determines a defect fluorescence of the semiconductor nanoparticles irrespective of a grain-size of the semiconductor nanoparticles; providing a fluorescent reagent comprising the semiconductor nanoparticles; binding sample biopolymers to probe biopolymers which have been immobilized on a substrate; electrostatically binding the semiconductor nanoparticles to sample biopolymers bound to the probe biopolymers; removing unbound probes from the sample polymers bound to the probe biopolymers and/or unbound semiconductor nanoparticles; and measuring said defect fluorescence of the semiconductor nanoparticles, which is exhibited due to the presence of a defect level mainly on a surface site of the semiconductor nanoparticles, thereby detecting an amount of the sample biopolymers bound to the probe biopolymers.