Patent ID: 6841352

Claim:
A method for determining whether a first test polypeptide interacts with a second test polypeptide in the presence or absence of one or more test compounds, comprising: providing a test yeast haploid cell recombinantly expressing a first fusion protein and a second fusion protein, said first fusion protein containing said first test polypeptide and said second fusion protein containing said second test polypeptide, wherein said test yeast haploid cell is either sterile or capable of mating depending upon the presence or absence of a protein-protein interaction between said first test polypeptide and said second test polypeptide; and determining whether said test yeast haploid cell is capable of mating to form a yeast diploid cell in the presence or absence of one or more test compounds, thereby detecting the presence or absence of protein-protein interaction between said first test polypeptide and said second test polypeptide in the presence or absence of said one or more test compounds.