Patent ID: 7094536

Claim:
A method of generating multiple copies of the complementary sequence of an RNA sequence of interest, said method comprising the steps of: (a) extending a first primer hybridized to a target RNA with an RNA-dependent DNA polymerase, whereby a complex comprising a first primer extension product and the target RNA is produced; (b) cleaving RNA in the complex of step (a) with an enzyme that cleaves RNA from an RNA/DNA hybrid; (c) extending a second primer hybridized to the first primer extension product with a DNA-dependent DNA polymerase, whereby a complex comprising the first primer extension product and a second primer extension product is produced; (d) denaturing the complex of step (c); and (e) hybridizing to the second primer extension product a propromoter polynucleotide comprising a propromoter and a region which hybridizes to the second primer extension product under conditions which allow transcription to occur by RNA polymerase, such that RNA transcripts are produced comprising sequences complementary to the target RNA; whereby multiple copies of the complementary sequence of the RNA sequence of interest are generated.