Patent ID: 8349885

Claim:
A method for preserving cells, tissues or organs using a composition comprising a compound of the following formula (1) or a pharmaceutically acceptable salt or stereoisomer thereof wherein X represents C or N, n is 0 or 1, and n is 1 when X is C and n is 0 when X is N, A represents a direct bond, C 3 -C 8 -cycloalkyl, phenyl, or 5˜6-membered heteroaryl or heterocycle, each of which includes 1˜3 heteroatoms selected from N, O and S atoms, R1 represents hydrogen, —C(O)—B—X′—R7 or —(CR5R6) m -B—X′—R7, m is an integer of 0 to 4, each of R5 and R6 independently represents hydrogen or C 1 -C 5 -alkyl, B represents a direct bond, C 3 -C 8 -cycloalkyl optionally containing oxo, or 3˜10-membered heterocycle or heteroaryl, each of which includes 1˜3 heteroatoms selected from O, S and N atoms, X′ represents a direct bond, —C(O)—, —SO 2 —, —CO 2 — or —C(O)NR5—, R7 represents hydrogen, C 1 -C 6 -alkyl, halogeno-C 1 -C 6 -alkyl, halogen, (CR5R6) m -phenyl, (CR5R6) m -hydroxy or (CR5R6) m -heterocycle where the heterocycle optionally contains oxo and is a 3˜10-membered ring including 1-3 heteroatoms selected from N, O and S atoms, R2 represents —(CR5R6) m -D-X″—R8, D represents a direct bond or a 3˜10-membered heterocycle or heteroaryl, each of which optionally contains oxo and is optionally fused, and includes 1˜4 heteroatoms selected from N, O and S atoms, X″ represents a direct bond, —C(O)—, —C(O)O—, —NR5C(O)—, —C(O)NR5- or —O—, R8 represents hydrogen, halogen, C 1 -C 6 -alkyl, halogeno-C 1 -C 6 -alkyl, tri(C 1 -C 6 -alkyl)silane or hydroxy-C 1 -C 6 -alkyl, R3 represents hydrogen, halogen, cyano, nitro, aryl-R9 or (CR5R6) m -D-R9, R9 represents hydrogen, halogen, C 1 -C 6 -alkyl, cyano, nitro or C 1 -C 6 -alkoxy, R4 represents —(CR5R6) m -Y-D-R10, Y represents a direct bond, —C(O)O— or —O—, and R10 represents hydrogen, nitro, halogen, C 1 -C 6 -alkyl, carboxy-C 1 -C 6 -alkyl, aryl or —C(O)O—R5, wherein each of said alkyl, alkoxy, aryl, cycloalkyl, heterocycle and heteroaryl is optionally substituted with one or more substituents selected from the group consisting of hydroxy, halogen, nitrile, amino, C 1 -C 6 -alkylamino, di(C 1 -C 6 -alkyl)amino, C 1 -C 6 -alkyl, halogeno-C 1 -C 6 -alkyl, C 1 -C 6 -alkylsulfonyl, aryl-C 1 -C 6 -alkoxy and oxo.