Patent ID: 8911937

Claim:
A method for detecting the methylation status of a CpG-containing nucleic acid in a sample comprising the steps of: a) providing said sample comprising a CpG-containing nucleic acid, b) denaturing said CpG-containing nucleic acid, thereby producing denatured CpG-containing nucleic acid, c) treating said denatured CpG-containing nucleic acid with bisulphite which modifies at least one unmethylated cytosine in said CpG-containing nucleic acid, thereby producing treated CpG-containing nucleic acid, d) amplifying said treated CpG-containing nucleic acid by means of at least one methylation-independent oligonucleotide primer, which comprises within the first 10 nucleotides of the 5′-end, at least one CpG dinucleotide in a region which hybridizes to the CpG-containing nucleic acid, wherein said at least one methylation-independent oligonucleotide primer hybridizes to both methylated and unmethylated CpG-containing nucleic acids after modification, and e) analyzing the amplified CpG-containing nucleic acid by melting curve analysis.