Patent ID: 7253334

Claim:
A method of cloning a non-human mammal, said method comprising the steps of: (a) permeabilizing a non-human mammalian cell, thereby generating a permeabilized cell having pores in its plasma membrane or a partial plasma membrane; (b) incubating said permeabilized cell in an extract from a mitotic cell under conditions that allow chromatin condensation and nuclear envelope breakdown of said permeabilized cell; (c) inserting said cell formed in step (b) into a nucleated or enucleated oocyte, thereby forming a reconstituted oocyte; and (d) transferring said reconstituted oocyte or an embryo formed from said reconstituted oocyte into the uterus of a host mammal under conditions that allows said reconstituted oocyte or said embryo to develop into a fetus.