Patent ID: 7704730

Claim:
A method for measuring analytes in a sample, the method comprising: a) contacting a first dilution of said sample with one or more binding surfaces in a measurement chamber; b) immobilizing an amount of a first analyte on said one or more binding surface(s) wherein at least one of said one or more binding surface(s) comprises a first binding reagent that binds the first analyte; c) binding a first detection reagent to said first analyte immobilized on said at least one of said one or more binding surface(s); d) contacting a second dilution of said sample with said one or more binding surfaces in said measurement chamber, said second dilution having a dilution factor less than said first dilution; e) immobilizing an amount of a second analyte on said one or more binding surface(s) wherein at least one of said one or more binding surface(s) comprises a second binding reagent that binds the second analyte; f) binding a second detection reagent to said second analyte immobilized on said at least one of said one or more binding surface(s); and g) measuring amounts of said first detection reagent and said second detection reagent bound to said binding surface(s).