Patent ID: 8889382

Claim:
A method for cultivating microorganisms of the order Thraustochytriales comprising the steps of: cultivating microorganisms selected from the group comprising Ulkenia sp. SAM 2179 or Schizochytrium sp. SR 21 in a fermentation medium containing CaCO 3 as an essential means for pH value stabilization, where content of CaCO 3 , in said fermentation medium is 7.5 g/l±0.5 g/l; wherein the pH value of the fermentation medium is set prior to the start of the fermentation in the range of 5 to 7 by adding a corresponding acid or base; isolating PUFAs from said microorganisms and/or said fermentation medium wherein the microorganisms bring forth a production of more than 10 wt % docosahexaenoic acid (DHA) per unit of weight of dry biomass.