Patent ID: 8427643

Claim:
A real-time polymerase chain reaction (PCR) monitoring apparatus, comprising: a condensing lens ( 103 ) for condensing an excitation light generated from a light source ( 100 ); a light tunnel ( 104 ) converting and transferring the excitation light condensed by the condensing lens ( 103 ) into a uniform surface light; a first band pass filter ( 105 ) for allowing a polarized light with a specific frequency of the surface light transferred from the light tunnel ( 104 ) that is matched with excitation properties of a fluorescent probe, to pass therethrough; a first polarizer ( 111 ) for polarizing the excitation light with a specific frequency passed through the first band pass filter ( 105 ); a polarizing beam splitter ( 108 ) for separating the polarized light with a specific frequency passed through first polarizer ( 111 ); a surface mirror ( 109 ) for transferring the polarized light divided from the polarizing beam splitter ( 108 ) to a sample, and reflecting the fluorescence generated from the sample to transfer the fluorescence to the polarizing beam splitter ( 108 ); a second polarizer ( 107 ) for polarizing the fluorescence so that the fluorescence reflected by the surface mirror ( 109 ) has the opposite component to the polarized light transferred to the polarizing beam splitter ( 108 ) and converted by the first polarizer ( 111 ); a second band pass filter ( 112 ) for allowing a fluorescence with a specific frequency of the polarized fluorescence transferred from the second polarizer ( 107 ) that is matched with an emitting properties of the fluorescent probe, to pass therethrough; a fluorescence detecting lens ( 113 ) for receiving the fluorescence passed through the second band pass filter ( 112 ); and a polarization converter ( 102 ) for converting the excitation light with a polarization component that would be removed by the first polarizer ( 111 ) into the excitation light with the polarization component capable of passing through the first polarizer ( 111 ), wherein the polarization converter 102 is provided between the light source 100 and the condensing lens 103 .