Patent ID: 8470563

Claim:
A method of amplifying a nucleic acid molecule comprising: forming a reaction mixture comprising a target nucleic acid molecule and at least one polymerase, the at least one polymerase comprising (i) SEQ ID NO: 30 or a variant thereof having at least 95% sequence identity to SEQ ID NO: 30, wherein position 708 of the variant of SEQ ID NO: 30 is leucine (L) or lysine (K) and the variant of SEQ ID NO: 30 has blood resistant polymerase activity or blood resistant polymerase activity and faster elongating polymerase activity or a fragment of (i) comprising leucine or lysine at the position corresponding to position 708 of SEQ ID NO: 30, wherein the fragment has blood resistant polymerase activity or blood resistant polymerase activity and faster elongating polymerase activity; and amplifying the target nucleic acid molecule in the reaction mixture.