Patent ID: 7132108

Claim:
A method for immunizing poultry from infection from Salmonella enteritidis which comprises administering to poultry a total dose of killed S. enteritidis of about 0.15 mg to about 15 mg, said dose administered as a single dose of a suspension of microspheres comprising killed S. enteritidis encapsulated in a porous polymeric shell comprising poly(lactide-coglycolide) copolymers; wherein the S. enteritidis is released from the microspheres in a biphasic pattern upon administration wherein about 20% to about 50% of the encapsulated S. enteritidis is released in a first phase in an initial burst within the first 7 to about 15 days of the administration of the suspension and the remainder of the S. enteritidis is released in a second phase over a period of about 10 to about 15 weeks.