Patent ID: 7510844

Claim:
An assay method for monitoring binding efficiency of L104EA29YIg to CD80 or CD86 receptor in a patient, said method comprising the steps of: (a) obtaining a blood sample from a patient dosed with L104EA29YIg, the blood sample including leukocyte cells; (b) adding CD14-FITC to the blood sample; (c) adding labeled competing anti-receptor antibody selected from the group consisting of mAb FUN-1, mAb IT2.2, mAb HA5.2B7, mAb L307.A and mAb MAB 104 and non-competing anti-receptor antibody selected from the group consisting of mAb 2D4 and mAb 1420 to the blood sample; (d) lysing the blood sample and fixing leukocytes; (e) isolating leukocytes from the lysed blood sample; (f) measuring the total receptor expression and available receptor from the leukocyte cells.