Patent ID: 8399189

Claim:
A method for simultaneous scanning and genotyping of a target nucleic acid comprising the steps of mixing the target nucleic acid with a first primer and a second primer to form a mixture, the primers configured for amplifying the target nucleic acid, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, wherein the probe element is 5′ of the template-specific primer region, amplifying the target nucleic acid to generate an amplicon, generating a melting curve for the amplicon by measuring fluorescence from a dsDNA binding dye as the mixture is heated, adjusting the mixture to favor hairpin formation by the probe element binding intramolecularly to the target nucleic acid, and generating a melting curve for the probe element by measuring fluorescence from the dsDNA binding dye as the mixture is heated.