Patent ID: 8084584

Claim:
A method for generating a nucleic acid encoding a mutant antibody, the method comprising: (a) identifying an antibody that is susceptible to deamidation, wherein the antibody includes a first region that is a complementarity determining region (CDR); (b) identifying an asparagine residue susceptible to deamidation within the first region; (c) identifying a glycine residue located adjacent and C-terminal to the asparagine residue; and (d) producing a recombinant nucleic acid molecule that encodes a mutant antibody, wherein the mutant antibody is identical to the antibody of (a) except for an alteration selected from the group consisting of (i) a deletion of the glycine residue, and (ii) a substitution of the glycine residue with an amino acid residue other than valine, isoleucine or proline, wherein the mutant antibody of (d) is less susceptible to deamidation than is the antibody of (a), and wherein the mutant antibody of (d) exhibits at least 70% of the antigen binding activity exhibited by the antibody of (a).