Patent ID: 7993824

Claim:
A method for determining whether a human immunodeficiency virus type 1 virus (HIV-1) has an increased likelihood of having a reduced susceptibility to treatment with amprenavir, comprising: detecting the presence or absence of a mutation in a protease associated with reduced susceptibility to treatment with amprenavir at amino acid position 11, 34, 76, 83, 91 or 95 of an amino acid sequence of said protease, wherein the mutation at position 11 is isoleucine (I) or leucine (L), the mutation at amino acid position 34 is glutamine (Q), the mutation at position 76 is valine (V), the mutation at position 83 is aspartic acid (D), the mutation at position 91 is alanine (A), valine (V), or serine (S), and the mutation at position 95 is phenylalanine (F), and determining whether the HIV-1 has an increased likelihood of having a reduced susceptibility to treatment with amprenavir, wherein the presence of said mutation indicates that the HIV-1 has an increased likelihood of having a reduced susceptibility to treatment with amprenavir, and wherein the level of susceptibility, mutations, and amino acid position number are compared to SEQ ID NO:1, the protease sequence of the NL4-3 reference strain.