Patent ID: 8057990

Claim:
A method for screening a compound or a salt thereof that alters a cell stimulating activity, comprising: measuring an intracellular calcium concentration increase activity of a GPR30 protein, (i) when a tacrine compound is brought in contact with the protein in a cell in the absence of a test compound, and (ii) when a tacrine compound is brought in contact with the protein in a cell in the presence of the test compound, wherein said GPR30 protein comprises the amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence having at least 80% homology to the amino acid sequence represented by SEQ ID NO: 1; comparing the intracellular calcium concentration increase activity between (i) and (ii); and selecting the compound that alters the intracellular calcium concentration increase activity; wherein the tacrine compound is a compound represented by formula: (in the formula, ring A represents an optionally substituted benzene; R 1 represents an optionally substituted amino; and each of R 2 and R 3 independently represents an optionally substituted hydrocarbon group, an optionally substituted heterocyclic group, or acyl; or R 2 and R 3 may form an optionally substituted 3- to 10-membered homo- or heterocyclic group with a carbon atom mutually bound and adjacent thereto) or a salt thereof.