Patent ID: 7598083

Claim:
A soluble composition, suitable for producing a final volume of cell culture media, wherein the composition comprises the following components in the following amounts per liter of the final volume of cell culture media: anhydrous CaCl 2 , 5-200 mg; anhydrous MgCl 2 , 15-50 mg; anhydrous MgSO 4 , 20-80 mg; FeSO 4 .7H 2 O, 0.05-0.50 mg; Fe(NO 3 ) 3 .9H 2 O, 0.01-0.08 mg; ZnSO 4 .7H 2 O, 0.40-1.20 mg; ferric ammonium citrate, 0.04-200 mg; KCl, 280-500 mg; NaCl, 5000-7500 mg; NaH 2 PO 4 .H 2 O, 30-100 mg; Na 2 HPO 4 , 30-100 mg; CuSO 4 .5H 2 O, 0.001-0.005 mg; CoCl 2 .6H 2 O, 0.001-0.10 mg; (NH 4 ) 6 Mo 7 O 24 4H 2 O, 0.001-0.005 mg; MnSO 4 .H 2 O, 0.000070-0.0080 mg; NiSO 4 .6H 2 O, 0.000025-0.0005 mg; Na 2 SeO 3 , 0.004-0.07 mg; Na 2 SiO 3 .9H 2 O, 0.02-0.4 mg; SnCl 2 .2H 2 O, 0.000025-0.0005 mg; NH 4 VO 3 , 0.0001-0.0025 mg; D-Glucose, 500-8000 mg; sodium pyruvate, 0.0-1000 mg; sodium hypoxanthine, 0.0-20.0 mg; glycine, 0.0-150 mg; L-alanine, 0.0-150 mg; L-arginine.HCl, 200-5000 mg; L-asparagine.H 2 O, 40-250 mg; L-aspartic acid, 20-1000 mg; L-cysteine.HCl H 2 O, 25.0-250 mg; L-cystine.2HCl, 15-150 mg; L-glutamic acid, 0-1000 mg; L-histidine.HCl.H 2 O, 100-500 mg; L-isoleucine, 50-1000 mg; L-leucine, 50-1000 mg; L-lysine.HCl, 100-1000 mg; L-methionine, 50-500 mg; L-ornithine.HCl, 0-100 mg; L-phenylalanine, 25-1000 mg; L-proline, 0-1000 mg; L-serine, 50-500 mg; L-taurine, 0-1000 mg; L-threonine, 50-600 mg; L-tryptophan, 2-500 mg; L-tyrosine.2Na.2H 2 O, 25-250 mg; L-valine, 100-1000 mg; d-biotin, 0.04-1.0 mg; D-calcium pantothenate, 0.1-5.0 mg; choline chloride, 1-100 mg; folic acid, 1-10 mg; i-Inositol, 10-1000 mg; nicotinamide, 0.5-30 mg; p-aminobenzoic acid, 0.1-20 mg; riboflavin, 0.05-5.0 mg; thiamine.HCl, 0.5-20 mg; thymidine, 0-3.0 mg; vitamin B 12 , 0.05-5.0 mg; linoleic acid, 0.01-2.0 mg; DL-α-lipoic acid, 0.03-1.0 mg; pyridoxine.HCl, 0.5-30 mg; putrescine.2HCl, 0.025-0.25 mg; and ethanolamine.HCl, 2-100 mg.