Patent ID: 7252962

Claim:
A method for detecting acetylcholine esterase sensitivity to insecticides in insects, comprising: homogenizing a single insect in a neutral to acid pH phosphate buffer and forming an insect homogenisate, providing a solution of acetylcholine iodide in a neutral to acid pH phosphate-buffered water-miscible organic solvent, providing a solution of 5,5-dithio-bis-(2-nitrobenzoic acid) in the phosphate buffer, providing a solution of propoxur in the phosphate buffer, introducing the insect homogenisate into at least one first well and at least one second well of an assay plate, then introducing the acetylcholine iodide and 5,5-dithio-bis-(2-nitrobenzoic acid) solutions into the at least one first well and 5,5-dithio-bis-(2-nitrobenzoic acid), acetylcholine iodide and propoxur solutions into the at least one second well, detecting a difference in yellow coloration between samples in the first and second cells, and correlating the yellow coloration difference observed in the first and second cells with acetylcholine esterase sensitivity to insecticide in the single insect.