Patent ID: 8849380

Claim:
A method of imaging human skin in vivo comprising: capturing a plurality of narrow spectral band illumination reflectance images of the human skin using a different combination of narrow spectral band illumination and detection filtering for each image; generating a chromophore absorption distribution map by processing the plurality of captured narrow spectral band illumination reflectance images to extract therefrom a distribution of at least one selected chromophore that absorbs at least one of an excitation and an emission of an endogenous target fluorophore, wherein processing the plurality of captured narrow spectral band illumination reflectance images includes determining a ratio between at least one of the plurality of captured narrow spectral band illumination reflectance images and a reference image exhibiting negligible absorption by the at least one selected chromophore; and generating a normalized fluorescence image by normalizing a fluorescence reflectance image of the endogenous target fluorophore with respect to the chromophore absorption distribution map.