Patent ID: 8153162

Claim:
A pharmaceutical composition comprising: (a) an AM preparation prepared from pulverized or ground amniotic membrane isolated from placenta that is frozen or previously frozen, comprising: i. high molecular weight hyaluronan (HA) that is cross-linked by a covalent bond to the heavy chain of inter-α-trypsin inhibitor (IαI), the high molecular weight HA having a molecular weight greater than 1000 kDa; ii. tumor necrosis factor-stimulated gene 6 (TSG-6); iii. pentraxin (PTX-3); and iv. thrombospondin (TSP-1); and (b) a pharmaceutically acceptable diluent, excipient, or carrier; wherein the ratio of total protein to HA in the pharmaceutical composition is less than 100 parts protein: 1 part HA.