Patent ID: 7534569

Claim:
A method of generating a single-stranded nucleic acid molecule, the method comprising: (a) combining in a mixture under conditions suitable for a polymerase extension reaction: (i) a polymerase, (ii) an initial polynucleotide comprising a 5′ portion and a 3′ portion, wherein the polynucleotide forms the nucleic acid molecule 5′ end; and (iii) a plurality of overlapping template oligonucleotides each having a 5′ template portion and a 3′ portion, wherein the 3′ portion of each template oligonucleotide is sufficiently complementary to the 3′ portion of one of: the initial polynucleotide or a 3′ portion of an extension polynucleotide formed by a polymerase extension reaction of the initial polynucleotide, (b) hybridizing the initial polynucleotide or the extension polynucleotide and one of the template oligonucleotides; (c) incubating the mixture for sufficient time to allow an extension polynucleotide to be synthesized; (d) adding a competimer that competes with the template oligonucleotide in step (b); (e) denaturing the extension polynucleotide and template oligonucleotide; and (f) repeating steps (b), (c), (d), and (e) to generate the single-stranded nucleic acid molecule, wherein the number of repeated cycles equals the number of different template oligonucleotides.