Patent ID: 7611837

Claim:
A kit for detecting haemagglutinin gene-containing influenza A subtype H5 virus in a biological sample comprising: (i) an isolating agent for isolating RNA molecules of the H5 virus from the biological sample and producing isolated RNA molecules therefrom; (ii) a nucleic acid amplifying agent for amplifying the isolated RNA molecules of the H5 virus and producing amplified target molecules for detection, wherein the nucleic acid amplifying agent includes: (a) a first purified and isolated DNA molecule consisting of: (1) a first DNA sequence consisting of any one of the DNA sequences of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3; and (2) a second DNA sequence encoding a promoter DNA sequence of a RNA polymerase; wherein the DNA sequences of SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3 are bindable at regions between 1107 to 1132, 1060 to 1140 and 1040 to 1160, of the haemagglutinin gene, respectively; and (b) a second purified and isolated DNA molecule consisting of: (1) a third DNA sequence encoding at least a portion of the RNA sequence of the H5 virus and consisting of any one of the DNA sequences of SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 9, SEQ ID NO. 10, and SEQ ID NO. 11; and (2) a fourth DNA sequence encoding a fifth DNA sequence for binding to a detection molecule; wherein the DNA sequences of SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO.7, SEQ ID NO.9, SEQ ID NO. 10 and SEQ ID NO. 11 are bindable at regions between 914 to 940, 866 to 961, 846 to 981, 1017 to 1042, 970 to 1063 and 950 to 1083, of the haemagglutinin gene, respectively; and (iii) a nucleic acid detecting agent for detecting the target molecule; wherein the nucleic acid detecting agent includes the detection molecule.