Patent ID: 7560249

Claim:
A method for the production of a heterologous protein by a fungus which method comprises a batch phase, an induction phase and a feed phase, said batch phase comprising growing fungal cells to a cell density of at least 5/gl on a batch medium comprising any carbon source, without specific preference; and said induction phase and feed phase comprising: (a) subsequently growing the fungal cells to a cell density of from 10 to 90 g/l, using a feed medium comprising an inducer and a carbon source wherein 50-100 wt. % of said carbon source is ethanol; then (b) creating limiting growth conditions by a method selected from the group comprising reducing the oxygen concentration in the fermenter medium to from 0 to below 30%; overfeeding the medium with ethanol until the ethanol level in the medium is at or above the growth limiting concentration for the cells of the strain that is grown in the medium; or decreasing the level of other essential ingredients for growth of the cells said ingredients being selected from nitrogen, phosphor, sulfur and vitamins; and then (c) further growing the cells on a medium comprising a carbon source wherein 50-100 wt. % of said carbon source is ethanol; with the proviso that the method does not relate to expression methods wherein ethanol is both used as inducer and as the carbon source.