Patent ID: 8877904

Claim:
A method for purifying antibodies from a solution containing antibodies, the method comprising a. contacting a first solution containing antibodies to a mixed mode chromatography matrix comprising an n-alkyl acid comprising 2-10 carbons, wherein the n-alkyl acid is linked directly to a hydroxy-functionalized solid support, thereby binding at least a portion of the antibodies to the matrix; b. separating the matrix with bound antibodies from the first solution; c. eluting the bound antibodies by raising the pH of a solution in contact with the matrix and bound antibodies to pH 6-8, thereby producing an elution solution at pH 6-8 comprising eluted antibodies; d. contacting the elution solution comprising eluted antibodies to a solid support matrix, the solid support matrix comprising hydroxyapatite (HT) or fluorapatite (FT), wherein at least a portion of the antibodies bind to the solid support matrix; e. separating the solid support matrix and bound antibodies from the elution solution; and f. eluting the bound antibodies from the solid support matrix, thereby purifying the antibodies from the solution.