Patent ID: 8703419

Claim:
A method for determining the methylation status of the 32 nd residue of the nucleic acid sequence of SEQ ID NO: 41 in genomic DNA, the method comprising the steps of: a) isolating genomic DNA from a clinical sample obtained from a liver cancer suspicious subject; and b) using a primer set to detect the methylation status, wherein said primer set is selected from the group consisting of: i) primer set 1 comprising a sense primer 20-30 nucleotides in length comprising the nucleic acid sequence of SEQ ID NO: 5 and an antisense primer 23-30 nucleotides in length comprising the nucleic acid sequence of SEQ ID NO: 6; ii) primer set 2 comprising a sense primer 24-30 nucleotides in length comprising the nucleic acid sequence of SEQ ID NO: 11 and an antisense primer 23-30 nucleotides in length comprising the nucleic acid sequence of SEQ ID NO: 12; and iii) primer set 3 comprising primer set 2 and a primer 21-30 nucleotides in length comprising the nucleic acid sequence of SEQ ID NO: 13.