Patent ID: 7960610

Claim:
A method for providing a transgenic plant with independently segregating transgenes comprising the steps of: a) introducing a DNA molecule into a plant cell, wherein said DNA molecule is a DNA plasmid consisting in linked order of a first Agrobacterium Ti plasmid border region operably linked to first DNA region comprising at least one transgene of agronomic interest which is operably linked to a second Agrobacterium Ti plasmid border region which is operably linked to a second DNA region comprising a positive selectable marker transgene linked to at least one plasmid maintenance element, wherein said second DNA region is also operably linked to said first Agrobacterium Ti plasmid border region, and whereby the DNA molecule provides for the integration of one or both of the DNA regions into a plant genome; and b) regenerating said plant cell into a transgenic plant by positive selection provided by expression of said positive selectable marker transgene; and c) selecting said transgenic plant for the presence of said transgene of agronomic interest; and d) screening said transgenic plant by a DNA detection method that identifies the linkage of said transgene of agronomic interest and said positive selectable marker transgene; and e) growing said transgenic plant into a fertile plant.