Patent ID: 8802373

Claim:
A method comprising: providing a sample potentially containing one or more target nucleotide sequences; forming primary products in a reaction process comprising a polymerase reaction, wherein each primary product comprises a 5′ primer-specific portion, a target nucleotide sequence-specific portion, and a 3′ primer-specific portion, wherein the 5′ primer-specific portion and the 3′ primer-specific portion differ from each other and are not the same as or complementary to the target-specific portion; providing one or more oligonucleotide primer sets, each oligonucleotide primer set characterized by a first and second oligonucleotide primer, wherein said first oligonucleotide primer is capable of hybridizing to the 3′ primer-specific portion of a primary product and said second oligonucleotide primer is capable of hybridizing to a 3′ primer specific portion of a complement of the primary product; and forming secondary products in a polymerase reaction mixture comprising the one or more oligonucleotide primer sets, wherein said secondary products are complementary to each of the formed primary products or complements thereof.