Patent ID: 8895246

Claim:
A method for detecting a target nucleic acid which comprises: detecting a sequence complementary to an arbitrary third base sequence by a hybridization with a sequence complementary to the third base sequence in a transcription product comprising: step 1 of subjecting the target nucleic acid as a template to reverse transcription using a first primer comprising a first base sequence complementary to a first sequence F1 of the target nucleic acid, a second base sequence complementary to a promoter sequence of RNA polymerase, and the arbitrary third base sequence which can hybridize with a base sequence complementary thereto, wherein the first base sequence, the second base sequence, and the third base sequence are in sequential order from the 3′ end of the first primer wherein, the template is a double stranded DNA; step 2 of subjecting the template of the target nucleic acid obtained in step 1 to digestion with an enzyme; step 3 of subjecting the reverse transcription product obtained from step 1 as a template to elongation using a second primer comprising a fourth base sequence identical to a second sequence of the target nucleic acid, wherein the second sequence of the target nucleic acid is located at a position closer to the 5′ end than the first sequence of the target nucleic acid; and step 4 of subjecting the elongation product obtained from step 3 to transcription with RNA polymerase to produce the transcription product and detecting a base sequence within the transcription product by a hybridization with a probe having the third base sequence, the base sequence detected within the transcription product being complementary to the third base sequence, wherein the sequence complementary to the third base sequence which is transcribed with RNA polymerase by a double-stranded DNA produced from the elongation reaction as a template, and wherein the promoter sequence is a double-stranded DNA constructed the first base sequence and the elongation product.