Patent ID: 8778610

Claim:
A method of preparing a plurality of amplifiable DNA molecules, comprising: providing a plurality of DNA molecules, said plurality having molecules comprising one or more regions that are GC-poor and having molecules comprising one or more regions that are GC-rich; subjecting the plurality to a first temperature such that the GC-poor regions are substantially denatured and such that the GC-rich regions are undenatured or are denatured only in part; subjecting the plurality to a second temperature such that at least part of the GC-poor regions incompletely renature and such that at least part of the GC-rich regions substantially completely renature, thereby producing renatured amplifiable GC-rich molecules; ligating an adaptor onto the end of at least some of the renatured GC-rich molecules to produce adaptor-ligated molecules, wherein the adaptor is further defined as a stem-loop oligonucleotide comprising an inverted repeat and a loop.