Patent ID: 7888098

Claim:
A process for the purification of a VLP from a recombinant bacterial host expressing said VLP, wherein said VLP comprises recombinant coat protein of an RNA bacteriophage, the process comprising the steps of: (a) homogenizing said bacterial host; (b) clarifying the homogenate obtained by said homogenizing; (c) purifying said VLP from the clarified homogenate obtained by said clarifying in a first chromatography comprising the steps of: (i) binding said VLP to a first chromatography matrix, wherein said first chromatography matrix is a tentacle anion exchange matrix; (ii) washing said first chromatography matrix; and (iii) eluting said VLP from said first chromatography matrix; and (d) further purifying said VLP from the eluate obtained by said first chromatography in a second chromatography, wherein said second chromatography is performed on a second chromatography matrix, wherein said second chromatography matrix is a hydroxyapatite matrix; wherein said steps are performed in the given order.