Patent ID: 7183046

Claim:
A method for determining whether a compound has the potential to inhibit cytokinesis by determining the compound's ability to inhibit the function of a CYK-4 protein comprising amino acid residues 360–536 of SEQ ID NO:2 to promote GTP hydrolysis by a Rho family GTPase, the method comprising: (i) incubating the Rho family GTPase with GTP for a period of time sufficient to allow saturation of the Rho family GTPase's GTP binding sites; (ii) adding a CYK-4 protein comprising amino acid residues 360–536 of SEQ ID NO:2 to the Rho family GTPase and the GTP in the presence and absence of the compound, wherein the CYK-4 protein, in the absence of the compound, stimulates GTP hydrolysis by the Rho family GTPase; and (iii) determining an amount of GTP that is hydrolyzed in the presence and absence of the compound; wherein the compound is determined to have the potential to inhibit cytokinesis if the compound inhibits the CYK-4 stimulated GTP hydrolysis determined in (iii).