Patent ID: 7828947

Claim:
A method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus comprising in the following order, an apparatus preparing step of preparing the apparatus comprising: a substrate; a first spacer disposed at one end of the substrate; a first thin film disposed on the substrate via the first spacer; a second spacer disposed at one end of the first thin film; a second thin film disposed on the first thin film via the second spacer; and a cover disposed at one end of the second thin film, wherein: a first chamber is formed between the substrate and the first thin film; the first thin film comprises a first through hole penetrating both surfaces thereof; a second chamber is formed between the first thin film and the second thin film; the second thin film comprises a second through hole penetrating both surfaces thereof; the cover has an inlet connected to the second through hole; the first through hole overlaps the second through hole in plan view; and the first chamber is connected to the inlet via the first through hole and the second through hole, a first electrolytic solution pouring step of pouring an electrolytic solution to the first chamber; a lipid solution pouring step of pouring a lipid solution through the second chamber to at least one of the first through hole or the second through hole; and a second electrolytic solution pouring step of pouring the electrolytic solution to the inlet to form the artificial lipid membrane inside the through hole to which the lipid solution is poured.