Patent ID: 7060469

Claim:
A method for elongating a functional acceptor, comprising the steps of: providing a functional acceptor, wherein the functional acceptor has at least two sugar units selected from the group consisting of GlcUA, GlcNAc, GalNAc, and hexosamine, and wherein the functional acceptor is attached to a substrate selected from the group consisting of silica, silicon, glass, polymers, organic compounds, metals and combinations thereof; providing a recombinant, soluble hyaluronic acid synthase having an empty acceptor site and being capable of elongating the functional acceptor, wherein the hyaluronic acid synthase is encoded by a nucleotide sequence capable of hybridizing at 68° C. In 5×SSC/5× Denhardt's solution/1.0% SDS, followed with washing in 0.2×SSC/0.1% SDS at room temperature to a complement of the hyaluronic acid synthase nucleotide sequence as set forth in SEQ ID NO: 2; and providing UDP-GlcUA and UDP-GlcNAc sugars such that the hyaluronic acid synthase elongates the functional acceptor.