Patent ID: 8470593

Claim:
An isolated host cell comprising a recombined amplifiable vector which is the product of a method comprising: (a) introducing one or more expression cassettes into a first multiple cloning site of a first universal transfer vector wherein said first universal transfer vector comprises the plasmid map set forth in FIG. 2 ; wherein the first multiple cloning site comprises nucleotides 620-766 of the nucleotide sequence set forth in SEQ ID NO: 2; and wherein said first multiple cloning site is in said orientation or in a reverse orientation; (b) introducing one or more expression cassettes, different from the cassettes introduced into said first universal transfer vector, into a second multiple cloning site of a second universal transfer vector which second multiple cloning site comprises nucleotides 620-772 of the nucleotide sequence set forth in SEQ ID NO: 1; wherein said second multiple cloning site is in said orientation or in a reverse orientation; and (c) introducing said cassettes from steps (a) and (b) into a third multiple cloning site of an amplifiable vector; wherein said amplifiable vector comprises the plasmid map set forth in FIG. 3 ; wherein said third multiple cloning site comprises nucleotides 5037-5183 of the nucleotide sequence set forth in SEQ ID NO: 3; wherein said third multiple cloning site is in said orientation or in a reverse orientation; and (d) introducing said amplifiable vector comprising said cassettes from steps (a) and (b) into a host cell.