Patent ID: 8062851

Claim:
A method for identifying a candidate compound that modulates protein-protein interaction between a Factor Inhibiting ATF4-mediated Transcription (FIAT) polypeptide and an activating transcription factor-4 (ATF4) polypeptide, the method comprising: (a) providing a first polypeptide that: (i) comprises a FIAT polypeptide or a fragment thereof; and (ii) displays ATF4-binding ability; (b) providing a second polypeptide that: (i) comprises an ATF4 polypeptide or a fragment thereof; and (ii) displays FIAT binding ability; (c) contacting the first and second polypeptides in the presence of a test compound; (d) determining the level of binding between the first and second polypeptides in the presence of the test compound; and (e) comparing the level of binding between the first and second polypeptides in the presence of the test compound with a level of binding between the first and second polypeptides in the absence of the test compound, wherein a different level of binding in the presence of the test compound than in its absence indicates that the test compound is a candidate compound that modulates protein-protein interaction between the FIAT polypeptide and the ATF4 polypeptide.