Patent ID: 7344712

Claim:
A method for providing a urinary tract tissue graft composition, comprising: providing a tissue culture frame; providing a segment of small intestinal submucosa having a mucosal surface and a serosal surface; positioning the segment of small intestinal submucosa in the tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame; isolating and culturing at least one bone marrow stromal cell from a tissue specimen of a subject; and seeding the at least one bone marrow stromal cell on a surface of the segment of small intestinal submucosa to form a urinary tract tissue graft composition; and culturing the urinary tract tissue graft composition under conditions that allow the at least one bone marrow stromal cell to differentiate into a smooth muscle-like cell and exhibit three-dimensional growth and matrix penetrance.