Patent ID: 7566814

Claim:
Plant cells containing a nucleic acid construct comprising a germline-specific plant promoter operatively associated with a site-specific recombinase coding sequence, wherein the genome thereof comprises a transcriptionally active selectable marker flanked by two recombination target sites; wherein said site-specific recombinase coding sequence operatively associated with said germline-specific plant promoter is selective for said recombination target sites flanking said selectable marker; wherein said germline-specific plant promoter is selected from the group consisting of the LAT52 gene promoter from tomato, the LAT56 gene promoter from tomato, the LAT59 gene promoter from tomato, the pollen-specific promoter of the Brassica S locus glycoprotein gene, and the pollen-specific promoter of the NTP303 gene; and wherein said site-specific recombinase coding sequence encodes a recombinase selected from the group consisting of Cre recombinase, FLP recombinase, and the R gene product of Zygosaccharomyces.