Patent ID: 7431685

Claim:
A method of preparing a substance library for investigating the molecular interaction of soluble or suspendable substances with solid-phase bounded peptidic or peptoid target molecules wherein the substance library comprises a plurality of parallel capillaries arranged in a plate, the method comprising the following steps: a) applying an organosilane layer on an internal surface of the capillaries, said organosilane layer having functional groups for anchoring peptidic or peptoid target molecules; b) defining sample areas wherein each sample areas comprises a plurality of adjacent capillaries, and wherein defining said sample areas is performed by position dependent application of a protecting substance temporarily protecting the functional groups of the organosilane layer of the capillaries within said sample areas or by position-dependent application of a protected anchor molecule within said sample areas; c) saturating the capillaries in the plate outside the sample areas with a deactivation reagent such that neither synthesis nor coupling of target molecules can occur in the capillaries outside the sample areas; d) deprotecting all protected functional groups or anchor molecules in the sample areas; and e) synthesizing peptidic or peptoid target molecules in the sample areas by pipetting solutions into the capillaries of the sample areas; wherein said solutions contain amino acids for sequential synthesis of peptide or peptoid target molecules or wherein said solutions are coupling solutions for coupling complete peptide or peptoid target molecules.