Patent ID: 8445247

Claim:
A process to stabilize the perhydrolysis activity of an enzyme when present in a formulation comprised of said enzyme and a carboxylic acid ester, the process comprising: (a) providing an aqueous formulation comprising: (i) at least one enzyme structurally classified as a CE-7 enzyme and having perhydrolysis activity and a signature motif comprising: (1) an RGQ motif at amino acid residues 118-120, (2) a GXSQG motif at residues 179-183, and (3) an HE motif at residues 298-299 when aligned to reference sequence SEQ ID NO:1 using CLUSTALW, said enzyme further having at least 30% amino acid identity to SEQ ID NO:1, (ii) at least one oligosaccharide excipient, and (iii) optionally at least one surfactant; and (b) spray-drying the aqueous formulation of (a) to produce an enzyme powder which substantially retains the perhydrolysis activity of the at least one enzyme when present in a formulation comprised of a carboxylic acid ester and the enzyme powder.