Patent ID: 8765179

Claim:
A process for coating a surface, comprising the following steps: (a) sequentially depositing on a surface at least one layer of alternate adsorbed polyelectrolytes to provide a coated surface presenting complementary amino and carboxylic reactive groups, wherein a first (or conversely second) polyelectrolyte is a cationic polymer comprising said amino groups and a second (or conversely first) polyelectrolyte is an anionic polymer comprising said carboxylic groups, (b) reacting said complementary reactive groups of the coated surface in the presence of a coupling agent, as to form amide bonds between said complementary reactive groups giving rise to a cross-linked polyelectrolyte multilayer film, and (c) treating said cross-linked polyelectrolyte multilayer film with a protein containing solution as to incorporate said protein on and inside said cross-linked polyelectrolyte multilayer film, wherein the obtained polyelectrolyte multilayers film has a thickness ranging from 500 nm to 20 μm.