Patent ID: 7247486

Claim:
A method for using an analytical device having a reusable centrifugal valve and an active surface, the method comprising: introducing a sample into a reaction chamber of the analytical device having said reusable centrifugal valve so that said sample contacts an active surface present in said reaction chamber, wherein said centrifugal valve includes an actuator and a bias means that exerts a counteracting force to hold said actuator in a stationary position; spinning the analytical device at a rotational speed to actuate the centrifugal valve from a stationary closed position to an open actuated position, the open actuated position opening the valve, thereby allowing the sample to exit from the reaction chamber through the valve; introducing a wash fluid into the reaction chamber; and spinning the analytical device at a rotational speed to actuate the valve from a closed position to the open actuated position, the open actuated position opening the valve and allowing the wash fluid to exit from the reaction chamber through the valve.