Patent ID: 8058035

Claim:
A method for producing an L-amino acid, the method comprising: culturing in a medium a bacterium belonging to the genus Escherichia or Pantoea , or a coryneform bacterium, and collecting the L-amino acid from the medium or bacterial cells; wherein said bacterium is able to produce an L-amino acid, and the activities of succinate dehydrogenase and Î±-ketoglutarate dehydrogenase are decreased in said bacterium by disrupting the genes encoding for succinate dehydrogenase and Î±-ketoglutarate dehydrogenase on the chromosome of the bacterium; wherein the gene encoding succinate dehydrogenase is selected from the group consisting of the sdhA gene, the sdhB gene, the sdhC gene and the sdhD gene, and combinations thereof; wherein the sdhA gene is selected from the group consisting of: (a) a DNA comprising the nucleotide sequence of nucleotides 1258 to 3021 in SEQ ID NO: 1, and (b) a DNA which is able to hybridize to a DNA comprising a sequence fully complementary to the entire nucleotide sequence of nucleotides 1258 to 3021 in SEQ ID NO: 1 under stringent conditions comprising washing at 0.1Ã—SSC, 0.1% SDS at 60Â°; wherein the sdhB gene is selected from the group consisting of: (c) a DNA comprising the nucleotide sequence of nucleotides 3039 to 3752 in SEQ ID NO: 1, and (d) a DNA which is able to hybridize to a DNA comprising a sequence fully complementary to the entire nucleotide sequence of nucleotides 3039 to 3752 in SEQ ID NO: 1 under stringent conditions comprising washing at 0.1Ã—SSC, 0.1% SDS at 60Â° C.; wherein the sdhC gene is selected from the group consisting of: (e) a DNA comprising the nucleotide sequence of nucleotides 527 to 913 in SEQ ID NO: 1, and (f) a DNA which is able to hybridize to a DNA comprising a sequence fully complementary to the entire nucleotide sequence of nucleotides 527 to 913 in SEQ ID NO: 1 under stringent conditions comprising washing at 0.1Ã—SSC, 0.1% SDS at 60Â° C.; and wherein the sdhD gene is selected from the group consisting of: (g) a DNA comprising the nucleotide sequence of nucleotides 910 to 1254 in SEQ ID NO: 1, and (h) a DNA which is able to hybridize to a DNA comprising a sequence fully complementary to the entire nucleotide sequence of nucleotides 910 to 1254 in SEQ ID NO: 1 under stringent conditions comprising washing at 0.1Ã—SSC, 0.1% SDS at 60Â° C.