Patent ID: 7982003

Claim:
An isolated recombinant bifunctional endolysin fusion protein, wherein said fusion protein comprises an endolysin comprising contiguous amino acid residues 1-443 of SEQ ID NO:2 from Group B Streptococcus B30 bacteriophage, wherein the endolysin comprises at least a D-alanyl-L-alanine endopeptidase amino terminus (CHAP) domain, and an Acm glycolase (lysozyme-like) domain, further comprising four mutations C26S, H91A, D158A and E185A, wherein the C-terminal end of the endolysin is fused in-frame to the N-terminal end of the lysostaphin comprising contiguous amino acid residues 1-246 of SEQ ID NO:4, wherein the lysostaphin further comprises two mutations at residues 125 and 232 to glutamine, wherein the fusion protein has antimicrobial activity and kills Staphylococcus aureus bacteria and Streptococcus agalactiae, S. dysgalactia , and S. uberis bacteria.