Patent ID: 7928292

Claim:
A method for producing a transgenic plant, comprising: (a) vortexing a solution comprising a germinating plant seedling, and at least one Agrobacterium strain that harbors a plasmid vector carrying a desired polynucleotide; (b) cultivating said Agrobacterium -transformed seedling in or on tissue culture medium; (c) producing a callus from said transformed seedling, and inducing shoot and root formation from said callus to produce a plantlet; (d) transferring said transformed plantlet to soil, and exposing said transformed plantlet to conditions that promote growth into a plant; and (e) screening said plant to determine if said desired polynucleotide is integrated into the genome of at least one cell of said plant, wherein said plant is stably transformed; wherein the vector comprises a T-DNA or P-DNA that comprises (i) the desired polynucleotide and (ii) a selectable marker gene operably linked to a terminator that is not naturally expressed in plants.