Patent ID: 8715932

Claim:
A method for analyzing a nucleic acid comprising, fragmenting a sample of DNA and circularizing the fragments of DNA, attaching the circularized fragments of DNA to a substrate surface in regions of the substrate surface that are capable of being monitored by sensors wherein each region is capable of being monitored by one sensor and a plurality of regions capable of being monitored by a sensor each comprise one attached circularized fragment of DNA, forming colonies of DNA from the attached circularized fragments of DNA wherein each of the colonies is a DNA molecule that is comprised of seven to 100 regions wherein the regions have the same sequence of nucleotides, hybridizing a set of primer molecules to each of the colonies wherein each of the primer molecules in the set of primer molecules has the same sequence of nucleotides, providing reactants to the DNA colonies comprising a nucleoside polyphosphate, comprising four to six phosphates, an enzyme capable of extending a DNA molecule, and an enzyme capable of deconstructing a DNA molecule under conditions that allow DNA to be extended and deconstructed, wherein the primer molecule is extended by a nucleoside polyphosphate complementary to a base of the attached DNA colony and deconstructed to remove the complementary nucleoside from the primer molecule a plurality of times; monitoring the sensors to detect increases in reaction products to determine the identity of the nucleoside that is complementary to a base of the attached DNA colony; terminating the primer molecule with an exonuclease resistant nucleoside polyphosphate comprising three to six phosphates having a base that has been identified as complementary to a base of the attached DNA strand; repeating the elements of providing reactants to the DNA colonies and monitoring the sensors at least one time; and determining sequence information for the sample of DNA to be sequenced based on increases in reaction products detected by the sensors.