Patent ID: 8377660

Claim:
A method for producing an optically active compound, comprising: reacting a nucleophile with a mixture of R- and S-stereoisomers of an azolide substrate by enzyme-catalyzed kinetic resolution so as to produce the optically active compound, wherein the azolide substrate contains an azole group used as a leaving group and an acyl group directly bonded to a nitrogen atom of the azole group, wherein the azolide substrate is represented by Formula (I): wherein X represents a halogen atom, methyl, ethyl, methoxy, etjpxu. —OH, —NH 2 , —NHCOCH 3 , —SH, or —SCH 3 ; R 1 is different from X and represents a straight-chain or branched saturated or unsaturated C 1 -C 20 aliphatic group, an aryl an aryloxy group or a C 3 -C 12 heterocyclic group containing one to three heteroatoms selected from the group consisting of O, S and N, each group being optionally substituted with one to three substituents selected from the group consisting of a halogen atom, an aryl group, an aryloxy group, —NH 2 , —CF 3 , —CN, —NO 2 , —OH, —SH, —COOH, —SCF 3 , —OCF 3 , —CONH 2 , —COC 6 H 5 , a C 1 -C 6 alkyl group and a C 1 -C 6 alkoxy group; R 2 represents an azole group optionally substituted with one substituent or two substituents selected from the group consisting of a halogen atom, an aryl group, an aryloxy group, —NH 2 , —CF 3 , —CN, —NO 2 , —OH, —SH, —COOH, —SCF 3 , —OCF 3 , —CONH 2 , a C 1 -C 6 alkyl group and a C 1 -C 6 alkoxy group; and n is 0 or 1.