Patent ID: 8715976

Claim:
A method for preparing a mutant E. coli having the ability to produce propanol in high concentration and high yield, the method comprising: (a) introducing or amplifying an ilvA gene in an E. coli having threonine-producing ability, wherein the ilvA gene is a threonine dehydratase-encoding gene; (b) introducing or amplifying an ackA gene, which encodes acetate kinase A or propionate kinase III that converts 2-ketobutyrate to propionate, atoD and atoA genes, which encodes acetyl-CoA:acetoacetyl-CoA synthase that converts propionate to propinyl-CoA, and an adhE third gene, which encodes an alcohol dehydrogenase that converts propinyl-CoA to propanol, in the E. coli; (c) introducing or amplifying an leuA gene, which encodes (R)-citramalate synthase that converts acetyl-CoA and pyruvate to 2-ketobutyrate, in the E. coli ; and (d) disrupting or inactivating genes encoding acetolactate synthase III and acetolactate synthase I in the E. coli.