Patent ID: 7951959

Claim:
At least one of a diastereomeric compound of the general formula I or formula II wherein formula I compounds are Ia1, Ia2, Ia3 and Ia4 and formula II compounds are IIa1, IIa2, IIa3 and IIa4 wherein each of R 1 and R 2 is the same or different and is independently selected from the group consisting of an aliphatic and heteroaliphatic group; each of L 1 to L 4 is the same or different and is independently selected from the group consisting of a divalent linear (—(CH 2 ) o —, o=1 to 15), crossed, or cyclic alkylene group which can be substituted by at least one atom selected from the group consisting of oxygen, substituted nitrogen, and sulfur; X 1 is selected from the group consisting of —OH, —SH, —NH 2 , —NH—NH 2 , —F, —Cl, —Br, —I, —NHS (hydroxysuccinimidyl/sulfosuccinimidyl), —O-STP (4-sulfo-2,3,5,6-tetrafluorophenoxy), —O-TFP (2,3,5,6-tetrafluorophenoxy), —O-benzotriazole, -benzotriazole, —NR-L-OH, —NR-L-O-phosphoramidite, —NR-L-SH, —NR-L-NH 2 , —NR-L-NH—NH 2 , —NR-L-CO 2 H, —NR-L-CO 2 —NHS, —NR-L-CO 2 -STP, —NR-L-CO 2 -TFP, —NR-L-CO-benzotriazole, —NR-L-CHO, —NR-L-maleimide, and —NR-L-NH—CO—CH 2 —I; R is —H or is equal to R 1 or R 2 ; L is equal to L 1 to L 4 ; each of X 2 and X 3 is the same or different and is independently selected from the group consisting of hydrogen, alkyl-, tert-alkyl-, aryl-, carboxyaryl-, dicarboxyaryl-, heteroaryl-, cycloalkyl-, heterocycloalkyl-, alkyloxy-, alkylmercapto-, aryloxy, arylmercapto, hydroxy-, amino-, nitro-, and cyano-residues, or is a solubilizing or ionizable substituent selected from the group consisting of —SO 3 − , —PO 3 2− , —CO 2 − , tert-ammonium, cyclodextrine, sugar, and combinations thereof; with the proviso that in Formula I, one of X 2 or X 3 is —SO 3 − ; in Formula II, one of X 4 or X 5 is hydrogen and the other is —SO 3 − ; M + is a number of Na + , K + , Ca 2+ , ammonia, or other cation(s) needed to compensate the negative charge brought by the cyanine; and n is an integer from 1 to 3.