Patent ID: 7749514

Claim:
A recombinant single-chain polypeptide comprising: a) a first amino acid sequence region comprising i) a first domain comprising a binding element comprising a galanin peptide able to preferentially interact with a galanin peptide receptor under physiological conditions; and ii) a second domain comprising a translocation element comprising a Clostridial neurotoxin translocation domain able to facilitate the transfer of a Clostridial toxin light chain across a vesicular membrane; b) a second amino acid sequence region comprising a therapeutic element comprising a Clostridial neurotoxin light chain having biological activity when released into the cytoplasm of said target cell; c) a third amino acid sequence region comprising an exogenous protease cleavage site from a tobacco vein mottling virus protease cleavage site, a human enterovirus 3c protease cleavage site, a subtilisin cleavage site, a SUMO/ULP-1 protease cleavage site, a papain cleavage site, or a non-human Caspase 3 protease cleavage site; wherein said first and second amino acid sequence regions are separated by said third amino acid sequence region.