Patent ID: 7869957

Claim:
A method of refining a biosystem reaction network, wherein the steps of said method are performed on a suitably programmed computer programmed to execute the steps comprising: (a) providing a mathematical representation of an actual biosystem; (b) reconciling said mathematical representation of said actual biosystem with a legacy data set or empirical data set; (c) determining differences between observed behavior of said actual biosystem and in silico behavior of said reconciled mathematical representation of said actual biosystem under similar conditions; (d) modifying a structure by deletion of a gene of a reaction network of said reconciled mathematical representation of said actual biosystem; (e) determining differences between said observed behavior of said actual biosystem and in silico behavior of said modified mathematical representation of said actual biosystem under similar conditions; and (f) providing a visual output to a user of accuracy or coverage of said modified mathematical representation, wherein accuracy indicates the percentage of said modified mathematical representation predictions in agreement with experimental data and coverage indicates the percentage of correct experimental changes predicted by said modified mathematical representation, thereby refining a biosystem reaction network.