Patent ID: 7738695

Claim:
A method for high-resolution image recording of at least one object with a microscope, comprising the steps of: positioning the at least one object in a receptacle arranged in an optical axis of the microscope, generating at least two first data sets per object, wherein: (a) the at least two first data sets represent intermediate images of the at least one object with at least two different predetermined orientations relative to the optical axis of the microscope, (b) the at least two different predetermined orientations of the object are provided by controlled movement of the at least one object relative to the receptacle, and (c) the controlled movement comprises a rotation of the at least one object by an influence of electrical field forces, said object being rotated around at least one of a predetermined axis and a predetermined rotation angle, and evaluating the data sets to obtain quantitative three-dimensional information of the at least one object, wherein said quantitative three-dimensional information represents a three-dimensional shape of the at least one object.