Patent ID: 7888086

Claim:
A method to immobilize a molecule of interest, comprising the steps of: (a) contacting a sample comprising (i) a fusion protein, the fusion protein comprising (1) a mutant dehalogenase having at least 85% sequence identity to SEQ ID NO:82 and having substrate binding activity, wherein the mutant dehalogenase comprises a substitution at an amino acid residue corresponding to residue 106 in the Rhodococcus dehalogenase having the amino acid sequence of SEQ ID NO:82, and wherein the amino acid substitution results in the mutant dehalogenase forming a bond with the dehalogenase substrate that is more stable than the bond formed between the polypeptide of SEQ ID NO:82 and the dehalogenase substrate and (2) a second protein, and (ii) a molecule of interest, wherein the molecule of interest is bound to the second protein with (iii) a solid support comprising a dehalogenase substrate; and (b) immobilizing the molecule of interest on the solid support as a result of a the bond formed between the mutant dehalogenase and the dehalogenase substrate.