Patent ID: 6949382

Claim:
An N-acetyl-L-cysteine cell culture medium (NAC medium) comprising; a buffered, serum-free solution having a pH value from about 6.8 to about 7.6, said solution containing: glucose; a biologically utilizable form of pantothenic acid or choline; at least one inorganic ion in a biologically utilizable form, wherein said ion is chloride ion, phosphate ion, calcium ion, magnesium ion, potassium ion, sodium ion, or iron ion; cumene hydroperoxide, wherein said cumene hydroperoxide is present in a concentration of about 5 μM to about 500 μM; deionized water, N-acetyl-L-cysteine (NAC); a mitogen wherein said mitogen stimulates said lymphocytes to grow; and optionally, at least one of a supplemental nutrient in a biological utilizable form wherein said supplemental nutrient is: a) an L-amino acid; b ) a vitamin; or c) at least one of pyruvate, adenine or inositol.