Patent ID: 8148137

Claim:
A mutant Escherichia coli ( E. coli ) strain for increasing production of L-valine, in which: (a) ilvA, leuA and panB are attenuated or deleted; (b) lacI gene is deleted; (c) feedback inhibition of ilvH gene is removed, wherein the removal of feedback inhibition of ilvH is induced by substitution of residues encoding glycine 14 and serine 17 to encode a small subunit of acetolactate synthase; (d) attenuators of ilvGMEDA and ilvBN operons are substituted with tac promoter; (e) aceF and pfkA genes are attenuated or deleted; (f) the mutant strain comprises an expression vector containing ilvB, ilvN, ilvC, ilvE and ilvD genes from E. coli; (g) the mutant strain overexpresses lrp gene of SEQ ID NO: 46; and (h) the mutant strain overexpresses a branched-chain amino acid exporter gene of SEQ ID NO:45.