Patent ID: 7547662

Claim:
A method of making a library of binding agent polypeptides comprising: generating a collection of random oligonucleotides, each random oligonucleotide comprising a random sequence of from about 6 to about 30 nucleotides (n), wherein n is A, C, G or T; substituting each random oligonucleotide into a generic binding agent nucleic acid present within an expression vector to generate a library of expression vectors that encode binding agent polypeptides; and placing the library of expression vectors into a population of host cells to generate a library of host cells that express a library of binding agent polypeptides; wherein the generic binding agent nucleic acid comprises the nucleic acids of SEQ ID NO:1 or the generic binding agent nucleic acid encodes a binding agent polypeptide with a sequence comprising the amino acids 1-26, the amino acids 34-53, the amino acids 59-74, the amino acids 79-101, the amino acids 110-116 and the amino acids 123-134 of SEQ ID NO:2.