Patent ID: 7029842

Claim:
A method for identifying a complete coding sequence of a gene of interest from a gene library, wherein the gene encodes a polypeptide carrying a signal sequence for secretion or partial secretion, the method comprising the steps of: (a) providing a genomic DNA library or a cDNA library; (b) inserting by in vitro transposition into a gene in said library a transposon comprising a polynucleotide encoding a promoterless and secretion signal-less secretion reporter; wherein there is a continuous open reading frame between the transposon and the polynucleotide encoding the secretion reporter; (c) introducing the library comprising the inserted transposon into a host cell; (d) screening for and selecting a host cell that secretes or partially secretes the secretion reporter; (e) identifying the coding sequence of the gene of interest into which the transposon was inserted in the selected host cell, by sequencing DNA flanking the inserted transposon; and (f) identifying the complete coding sequence of the gene of interest identified in step (e) by sequencing.