Patent ID: 8343566

Claim:
A process of preparing a canola protein isolate having a protein content of at least about 90 wt % (N×6.25) on a dry weight basis, which comprises: adding an initial quantity of a calcium salt to supernatant from the precipitation of a canola protein micellar mass to provide conductivity of about 1 to about 3.5 and insufficient to cause precipitation of calcium phytate, adjusting the pH of the resulting solution to about 2.0 to about 4.0 to form an acidified solution, partially concentrating the acidified solution to a protein concentration of about 50 g/L or less, adding a further quantity of a calcium salt to provide a conductivity of about 4 mS to about 30 mS, and to cause a precipitate to form, removing the precipitate from the resulting solution to provide a clear pH-adjusted solution, concentrating the clear, pH-adjusted solution to a protein content of at least about 50 g/L to produce a clear concentrated canola protein solution, optionally diafiltering the clear canola protein solution, optionally effecting a colour removal step, and drying the concentrated protein solution.