Patent ID: 7638296

Claim:
A method for obtaining human erythropoietin comprising: (a) culturing and expanding mammalian cells selected from the group consisting of CHO, COS, BHK, Namalwa, and HeLa cells which express recombinant human erythropoietin in culture medium comprising serum; (b) culturing said cells of step (a) in culture medium consisting of: (i) DMEM (Dulbecco's modified Eagle's medium); (ii) F12 medium; (iii) insulin; and (iv) NaHCO 3 , glucose, lactose, galactose, ethanolamine, pyruvate, glutamine, tryptophan, asparagine, and serine as additives; (c) separating supernatant containing human erythropoietin from said cells of step (b); and (d) concentrating said supernatant and recovering human erythropoietin from said supernatant.