Patent ID: 8728983

Claim:
A minimized phage display vector comprising at least a cloning cassette, a phage display cassette and a bacterial cassette, said minimized phage display vector characterized in that the cloning cassette comprises a nucleic acid sequence encoding a polypeptide corresponding at least to the intra-domain loop of a constant domain of an antibody, said minimized phage display vector having a size of 3300 base pairs or less, said minimized phage display vector characterized in that the cloning cassette comprises at least a nucleic acid sequence encoding a promoter and a nucleic acid sequence encoding a signal peptide, characterized in that said promoter is selected from the group consisting of Lac, Tac, Trp, Tet, T7, SP6, characterized in that said signal peptide is selected from the group consisting of pelB, geneIII, phoA, malE, dsbA, said minimized phage display vector characterized in that the cloning cassette further comprises a nucleic acid sequence encoding a cloning site, said minimized phage display vector characterized in that the phage display cassette comprises a nucleic acid sequence comprising at least the C-terminal domain of M13 gene III protein corresponding to the SEQ ID No 132 or 133, said minimized phage display vector characterized in that the phage display cassette further comprises a nucleic acid sequence encoding a stop codon, a nucleic acid sequence encoding a phage replication origin, and a nucleic acid sequence encoding an encapsulation or packaging sequence, and said minimized phage display vector characterized in that the bacterial cassette comprises at least a nucleic acid sequence encoding a promoter, a nucleic acid sequence encoding a positive selective marker and a nucleic acid sequence encoding a replication origin, characterized in that said promoter is a beta lactamase promoter, characterized in that said positive selective marker is an ampicillin or a chloramphenicol resistance gene.