Patent ID: 7193126

Claim:
A method for generating Drosophila fly stocks carrying a mutant allele of a wild-type transgene of interest, the method comprising: a. misexpressing the wild-type transgene of interest in Drosophila flies, using a GAL4/UAS expression system; b. determining the phenotypic consequence of such misexpression, and selecting flies in which the phenotype is displayed as carriers of the transgene of interest; c. mutagenizing the wild-type transgene in the selected flies to produce carriers of a mutant transgene that differs in structure from the wild-type transgene; d. causing the mutant transgene to be expressed in the selected flies; e. determining whether expression of the mutant transgene produces a new dominant phenotype in the flies; f. selecting male flies for mating which carry the mutant transgene and display any such new dominant phenotype; and g. mating said male flies en masse to female flies to establish stable stocks of Drosophila flies carrying a mutant allele of the transgene of interest.