Patent ID: 7932047

Claim:
A method for detecting neoplastic disorders from a solubilized body sample of a human subject, the method comprises the steps of: (a) obtaining a liquid-based cytological (LBC) body sample in a preservative solution comprising an alcohol and metal ions from a human subject, (b) solubilizing the body sample in a lysis medium, and (c) determining the overexpression of a marker molecule selected from the group consisting of: i) a cyclin dependent kinase inhibitor selected from the group consisting of p16 INK4a , p13.5, p14, p15 INK4b , p18 INK4c , p19 INK4d , p21 WAF1/CIP1 , and p27 KIP1 ; and ii) the cell cycle regulatory protein p14 ARF ; in the solubilized body sample by comparing the level of the marker molecule within said solubilized body sample with the level of the marker molecule present in a solubilized healthy human body sample.