Patent ID: 8268622

Claim:
A method for producing a non-transgenic, herbicide resistant or tolerant plant comprising: introducing into plant cells a recombinagenic oligonucleobase with a targeted mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses a EPSPS protein that is mutated at amino acid positions Thr 179 and Pro 183 in an Arabidopsis EPSPS protein (SEQ ID NO:19) or at an analogous amino acid residue in an EPSPS homolog wherein Thr 179 is changed to Ile and Pro 183 is changed to Thr or Ala; selecting a plant cell exhibiting improved tolerance to glyphosate as compared to a corresponding wild-type plant cell; and regenerating a nort-transgenic herbicide resistant or tolerant plant having as mutated EPSPS gene from said selected plant cell.