Patent ID: 8828958

Claim:
A single-stranded DNA expression cassette comprising in 5′ to 3′ order, a promoter, a primer binding site and an expression termination sequence, having one or more of the following sequences inserted between the promoter and the primer binding site: [SEQ ID NO: 18] TTTGATGACC TTTGCTGACC ATACAATTGC GATATCGTGG [SEQ ID NO: 71] TTTGATGACC TTTGCTGACC ATACAATTGC GATAT [SEQ ID NO: 72] TTTGATGACC TTTGCTGACC ATACAATTGC [SEQ ID NO: 76] TGACCTTTGC TGACCATACA ATTGCGATAT CGTGG [SEQ ID NO: 77] TTTGCTGACC ATACAATTGC GATATCGTGG [SEQ ID NO: 78] TGACCATACA ATTGCGATAT CGTGG [SEQ ID NO: 79] ATACAATTGC GATATCGTGG [SEQ ID NO: 84] ATACAATTGC GATATC [SEQ ID NO: 85] ATACAATTGC GATATCGT.