Patent ID: 8318694

Claim:
A process for preparing an oligosaccharide derivative of the formulae (1), (3) to (6) from an oligosaccharide mixture, the process being characterized in that the process comprises the steps of (a) introducing a lipophilic group into oligosaccharides of the mixture to obtain a mixture of oligosaccharide derivatives, and (b) treating the oligosaccharide derivative mixture by serotonin affinity column chromatography wherein R 1 is 2-carboxyphenyl, 3-carboxyphenyl, 4-carboxyphenyl, p-ethoxycarbonylphenyl or 2-pyridyl, —R 2 of formula (4) is -Asn-R 3 , and R 2 of formula (6) is hydroxyl, -Asn or -Asn-R 3 , wherein Asn is asparagine, R 3 is a carbamate-type or amide-type protective group, and Ac is acetyl.