Patent ID: 7892562

Claim:
A method of stimulating a Th-1 immune response to human immunodeficiency virus type 1 (HIV-1) in a mammalian subject, which method comprises: i) Administering to the mammalian subject at least three doses of an immunogenic composition wherein the immunogenic composition comprises a whole inactivated HIV-1 virus, wherein the virus has been inactivated by exposure to ultraviolet radiation and psoralen, lacks CD55 and CD59 in the viral membrane and has been subjected to desialation, and wherein the time between administration of the first and second doses of the immunogenic composition is from 1 to 20 days and the time between administration of the second and third doses is from 120 to 180 days; further wherein each dose of the immunogenic composition is a ten fold dilution in concentration of the previous dose; and ii) Administering to the mammalian subject an adjuvant to boost the Th-1 immune response, wherein the time between the first and second administration of the adjuvant is from 1 to 20 days and the time between the second and third administration is from 120 to 180 days.