Patent ID: 8221804

Claim:
A method of treating a disease, condition, or disorder selected from the group consisting of a cardiac disorder; atherosclerosis; a neurodegenerative disorder; a cerebrovascular disorder; a neuropsychiatric disorder; diabetes: and a combination thereof, the method comprising steps of: administering to a subject in need thereof an effective amount of a composition comprising a botanical extract prepared from a botanical raw material selected from the group consisting of coffea , ginkgo, garlic, bacopa, butcher's broom, flaxseed oil, ginseng, grape seed, St. John's wort, artichoke powder, tea leaf, tobacco, cocoa, and a combination thereof, wherein the composition comprising the botanical extract is prepared by a process comprising steps of: (a) solubilizing the botanical raw material in a polar solvent below a pH of about 5 to yield a solubilized botanical material; (b) partitioning the solubilized botanical material between an aqueous phase and an organic phase corresponding to the polar solvent above a pH of about 10 to yield a partitioned botanical material in the organic phase; (c) separating the partitioned botanical material into a plurality of fractions; (d) assessing each fraction of the plurality of fractions to identify an activity inhibiting demethylation of a protein phosphatase 2A (PP2A) enzyme in at least one fraction; and (e) collecting the at least one fraction containing the activity inhibiting demethylation of the protein phosphatase 2A (PP2A) enzyme or the combination of fractions containing the activity inhibiting demethylation of the protein phosphatase 2A (PP2A) enzymes to constitute the composition, wherein the composition is further characterized as having the following properties: (1) the botanical extract is essentially free of chlorogenic acid; and (2) the botanical extract contains at least 5 units per microliter of the activity inhibiting demethylation of the protein phosphatase 2A (PP2A) enzyme and thereby stimulates methylation of the protein phosphatase 2A (PP2A) enzyme.