Patent ID: 6998249

Claim:
A method of determining a level of toxicity of a chemical compound comprising: a) culturing cells in the presence of a plurality of concentrations of said chemical compound; b) measuring a first indicator of cell health at four or more concentrations of said chemical compound; c) measuring a second indicator of cell health at four or more concentrations of said chemical compound; d) measuring a third indicator of cell health at four or more concentrations of said chemical compound; e) determining the level of toxicity of said chemical compound from the measurements taken in steps (b), (c) and (d) by i) performing a dose response analysis of for each indicator of cell health from the measurements taken in steps (b), (c) and (d); ii) identifying, from the dose response analyses, the highest concentration of said chemical compound at which no measurable toxic effect was observed for each measured indicator of cell health; iii) selecting, as the toxic concentration (C tox ), a concentration less than or equal to the highest concentration of said chemical compound at which no measurable toxic effect was observed for all measured indicators of cell health, as a level of toxicity for the chemical compound.