Patent ID: 7632983

Claim:
A method of producing a recombinant monoclonal antibody having effector function in a duckweed plant culture or a duckweed nodule culture, comprising the steps of: (a) culturing within a duckweed culture medium a duckweed plant culture or a duckweed nodule culture, wherein said duckweed plant culture or said duckweed nodule culture is stably transformed to express said monoclonal antibody, and wherein said monoclonal antibody is expressed from one or more nucleotide sequences comprising a coding sequence for a chain of the monoclonal antibody, an operably linked coding sequence for a signal peptide that directs secretion of the monoclonal antibody, an operably linked 5′ leader sequence consisting of SEQ ID NO:16, and an operably linked plant intron sequence that is inserted upstream of the coding sequence; and (b) collecting said monoclonal antibody from the duckweed culture medium, the duckweed plant culture, or the duckweed nodule culture, wherein said coding sequence for the chain of the monoclonal antibody comprises between 70-100% Lemna gibba -preferred codons or Lemna minor -preferred codons.