Patent ID: 7399630

Claim:
A cell culture vessel for culturing adherent cells, said vessel comprising a polymer surface, a hyaluronic acid (HA) layer bonded to said polymer surface, and one or more peptides covalently bound to the HA layer, wherein said peptides promote adherence of cells that do not adhere to the HA layer in the absence of said peptides, and wherein each of said one or more peptides comprises: (a) one or more peptide sequences selected from the group consisting of SEQ ID NO:2 (EFIKY), SEQ ID NO:3 (EEEKV), SEQ ID NO:4 (GHK), SEQ ID NO:6 (KKLVY), SEQ ID NO:8 (AFAFK), SEQ ID NO:10 (KMLIY), SEQ ID NO:11 (KSYYY), SEQ ID NO:12 (AKKMV), SEQ ID NO:14 (AFIPV), SEQ ID NO:15 (KKMYY), and SEQ ID NO:16 (AIKKK); (b) a chemically or recombinantly prepared peptide multimer that includes at least two repeats of any one or a combination of SEQ ID NOs:2-4, 6, 8, 10-12, and 14-16; or (c) a cell adhesion promoting variant of any one or more of SEQ ID NOs:2-4, 6, 8, 10-12, and 14-16, said variant having a conservative substitution of a single amino acid residue, wherein said variant has the ability to promote cell attachment or growth.