Patent ID: 7919279

Claim:
A high-throughput method for determining a killer cell immunoglobulin-like receptor (KIR) genotype of a subject, comprising: co-amplifying a plurality of target sequences of a plurality of query KIR genes and a target sequence of at least one anchor KIR gene selected from the group consisting of 3DL3, 3DL2, 2DL4, and 3DP1, wherein the target sequences are suspected of comprising single nucleotide polymorphisms (SNPs) and wherein the amplification is by using a plurality of 3′ primers and a plurality of 5′ primers, wherein each pair of 3′ primer and 5′ primer are selected to amplify a sequence flanking the SNP, extending a plurality of extension primers in an extension reaction, wherein each extension primer is complementary to a nucleic acid sequence of an amplified target sequence at a 5′ end of a SNP wherein the extension primer for 3DL3 is SEQ ID NO:196 or SEQ ID NO:203, the extension primer for 3DL2 is SEQ ID NO: 211 or SEQ ID NO: 197, the extension primer for 3DL3 is SEQ ID NO:194 OR SEQ ID NO:195, and the extension primer for 3DP1 is SEQ ID NO:214, and detecting in the extension reaction the presence or absence of the plurality of SNPs of the plurality of query KIR genes and the presence of a SNP of the at least one anchor KIR gene by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, wherein detection of the presence or absence of the plurality of SNPs of query KIR genes is indicative of the KIR genotype of the subject.