Patent ID: 8703728

Claim:
A method of reducing target messenger RNA levels in an animal comprising administering to said animal an oligomeric compound comprising a sequence of linked nucleosides and nucleoside analogs which is 100% complementary to said target messenger RNA and has the structure: T 1 -(Nu 1 -L 1 ) n1 -(Nu 2 -L 2 ) n2 -(L 3 -NU 3 ) n3 -T 2 wherein: each Nu 1 and Nu 3 is, independently, a high affinity modified nucleoside,wherein at least one of Nu 1 and Nu 3 is a bicyclic nucleoside analog; each Nu 2 is a 2′-deoxy nucleoside; each L 1 , L 2 is a phosporothioate internucleoside linking group; each L 3 is a phosphorothioate internucleoside linking group except that one of L 3 is a single bond when attached to L 2 1 each T 1 and T 2 is, independently, H or an optionally linked conjugate group; n1 is from 1 to about 3; n2 is from 12 to about 16; and n3 is from 1 to about 3 wherein the total of n1, n2 and n3 is 14 to 21.