Patent ID: 7105661

Claim:
Process for manufacturing a nucleic acid fragment comprising enzymatically incorporating at the 3′ OH end of the nucleic acid fragment a modified morpholino-nucleotide having as precursor a compound of formula: in which R 1 represents a nucleic base, m is 0 or land R 2 is selected from the group consisting of: —(CH 2 ) n —NH 2 —(CH 2 ) n —SH —(CH 2 ) n —COOH —(CH 2 ) n —OH —(CH 2 ) n —NH—R 3 —(CH 2 ) n —SR 3 —(CH 2 ) n —CO—R 3 and —(CH 2 ) n OR 3 in which n is an integer ranging from 1 to 12 and R 3 is selected from the group consisting of a label, a protein, an enzyme, a fatty acid, and a peptide.