Patent ID: 6852705

Claim:
A method for obtaining an immunogenic response comprising administering to a bovine or porcine: (a) a DNA vaccine or immunogenic or immunological composition against a pathogen of a bovine or porcine comprising: (i) a plasmid containing and expressing a nucleotide sequence encoding an immunogen of a pathogen of the bovine or porcine; and (ii) a cationic lipid containing a quaternary ammonium salt, of formula in which R 1 is a saturated or unsaturated linear aliphatic radical having 12 to 18 carbon atoms, R 2 is another aliphatic radical containing 2 or 3 carbon atoms, and X a hydroxyl or amine group; and (b) an inactivated, attenuated live, subunit or recombinant vaccine or immunogenic or immunological composition against a bovine or porcine pathogen, wherein (a) and (b) are administered together in a combination or sequentially.