Patent ID: 7192722

Claim:
A method of preparing a hapten of Formula I suitable for use in raising an antibody capable of binding with at least one structural epitope of a phenethanolamine, the method comprising the steps of: preparing a phenethanolamine derivative of the formula D by reacting octopamine hydrochloride with Z 1 X 1 , in which Z 1 is a crosslinker, the crosslinker being —R—X 2 , in which R is a bivalent link and X 2 is a functional group containing at least one hetero-atom capable of linking the R of the crosslinker with a moiety selected from the group comprising an antigenticity-conferring carrier material and a detectable labelling agent, and X 1 is a halide; and reacting the phenethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E, in which Z 2 is H; Y is independently H or CH 3 ; W is independently H or CH 3 ; n is independently 1 or 2; and m is independently 0 or 1