Patent ID: 7122304

Claim:
A method of producing one or more cDNA molecules comprising: (a) contacting a sample comprising a cell or a virus with a solid medium, wherein: (i) the sample comprises mRNA and genomic DNA; (ii) the mRNA comprises an mRNA-template of interest; and (iii) wherein the solid medium comprises: a matrix; and a composition for inhibiting degradation of the mRNA template, wherein: the composition comprises a detergent or surfactant; and the composition is sorbed to the matrix then dried prior to contact with the sample; (b) sorbing at least a portion of the mRNA template to the solid medium; (c) eluting the mRNA from the solid medium while retaining the genomic DNA; and (d) contacting the mRNA with one or more reverse transcriptases under conditions sufficient to synthesize one or more cDNA molecules complementary to all or a portion of the mRNA template of interest.