Patent ID: 7074984

Claim:
A process for obtaining a cross-incompatible inbred maize plant comprising a gene cluster that encodes for a Teosinte Crossing Barrier (TCB) trait, which when crossed with a second inbred maize plant, produces a hybrid maize plant which is cross-incompatible and contains a gene cluster that encodes for a TCB trait, the process comprising the steps of: a) crossing a first donor parental maize plant comprising a gene cluster that encodes for a TCB trait with a second donor parental maize plant containing genes that encode for desirable traits in hybrid combination, wherein the gene cluster in said first donor parental maize plant comprises a Tcb locus and at least one modifier gene and further wherein said first donor parental maize plant is derived from a maize plant designated W22-TCB, seeds of which have been deposited as ATCC No. PTA-1601; b) collecting the seed resulting from the cross in step a); c) planting and growing the seed collected in step b) under plant growth conditions; d) obtaining DNA from each of the plant of the plant population grown in step c); e) analyzing the DNA obtained from each plant in step d) using at least one molecular marker including and between molecular markers umc1117 and bnlg490 shown in FIG. 3B to identify a Tcb locus and at least one modifier gene; f) selecting plants from said population having the Tcb locus and at least one modifier gene for further crossings and screenings; g) repeating steps d)â€“f) until a line is obtained which is homozygous for the gene cluster for cross-incompatability to provide such a trait in an inbred maize plant to be used in hybrid combination; and h) selecting plants from said population having the gene cluster for cross-incompatibility for further crossings and screenings until a line is obtained which is homozygous for the gene cluster for cross-incompatibility to provide such a trait in an inbred maize plant to be used in hybrid combination.