Patent ID: 8067212

Claim:
An isolated Bacillus pumilus strain that produces a high yield of tetramethylpyrazine wherein the Bacillus pumilus strain is characterized by the following properties: a rod-shaped bacterium with a length of 1.5 to 3.0 μm and a diameter of 0.6 to 0.7 μm; a colony color of milky-white; a capability of sporulation; a positive VP reaction; an ability to produce acid when cultured with glucose, arabinose, xylose, mannitol, or fructose; an ability to hydrolyze casein, gelatin, and Tween 80; an ability to produce a high yield of tetramethylpyrazine with glucose as a substrate in a fermentation medium; an inability to hydrolyze esculine; an ability to utilize citrate as the carbon source; an ability to live in 10% NaCl solution (w/w) and grow under 50° C.; and a 16s rDNA sequence that shares a similarity of 99% with other Bacillus pumilus strains wherein the isolated Bacillus pumilus strain is designated Bacillus pumilus RX3-17, which is deposited in China Center for Type Culture Collection with the deposit number CCTCC M 206043.