Patent ID: 7183118

Claim:
A method for identifying and quantifying a glycopolypeptide in a sample, comprising: (a) derivatizing carbohydrate groups of glycopolypeptides in a polypeptide sample; (b) immobilizing said derivatized carbohydrate groups of said glycopolypeptides to a solid support; (c) cleaving said immobilized glycopolypeptides, thereby releasing non-glycosylated peptide fragments and retaining immobilized glycopeptide fragments; (d) labeling said immobilized glycopeptide fragments with an isotope tag; (e) releasing said glycopeptide fragments from said solid support, thereby generating released glycopeptide fragments; (f) analyzing said released glycopeptide fragments using mass spectrometry; (g) identifying a released glycopeptide fragment; (h) quantifying the amount of said glycopeptide fragment identified in step (g); (i) identifying the glycopolypeptide from which the glycopeptide fragment in step (g) was derived, thereby identifying and quantifying the glycopolypeptide.