Patent ID: 8852909

Claim:
An engineered ketoreductase polypeptide derived from a wild-type Lactobacillus ketoreductase of sequence of SEQ ID NO: 2, 4, or 98, which is capable of stereoselectively reducing acetophenone to (S)-1-phenethanol with a percent stereomeric excess of at least 90%, wherein the engineered polypeptide comprises: an amino acid sequence that is at least 90% identical to the sequence of SEQ ID NO: 2, 4, or 98; an alanine or proline residue at a position corresponding to position 190 of SEQ ID NO: 2, 4, or 98; and an isoleucine, leucine, or methionine residue at a position corresponding to position 196 of SEQ ID NO: 2, 4, or 98.