Patent ID: 8765925

Claim:
An improved method for preparing Timosaponin BII, the method comprising: a) providing the decoction pieces of Chinese traditional medicine Rhizoma Anemarrhenae or fresh rhizome or fibrous root of Anemarrhena asphodeloides Bunge as raw material and extracting with a solvent selected from the group consisting of water, acetone, ethanol, and the mixture of at least two of them; b) separating the extract solution obtained from a) by one or more methods selected from the group consisting of resin adsorption, polyamide chromatography and reversed-phase column chromatography, and eluting by an eluant selected from the group consisting of water, acetone, ethanol and a mixture of at least two of them; c) combining the eluates of b), drying and obtaining Timosaponin BII; wherein the method for preparing Timosaponin BII does not use n-butanol, methanol or normal phase silica gel column chromatography.