Patent ID: 7947493

Claim:
A nucleic acid construct comprising: a DNA dependent RNA polymerase promoter; a 2A cardiovirus polynucleotide sequence encoding a 2A polypeptide, wherein the 2A polynucleotide is operably linked to the promoter, wherein the 2A polypeptide is selected from the group consisting of SEQ ID NOs: 43, 44, 45, 46, 47, 48, 49, and 50; a first foreign polynucleotide encoding a detectable polypeptide, wherein the first foreign polynucleotide is immediately downstream of and operably linked to the 2A polynucleotide; an IRES polynucleotide sequence, wherein the IRES sequence is immediately downstream of the first foreign polynucleotide; and a second foreign polynucleotide encoding a detectable polypeptide, wherein the second foreign polynucleotide is immediately downstream of and operably linked to the IRES sequence.