Patent ID: 8771957

Claim:
A method of sequencing one or more target polynucleotides, the method comprising the steps of: providing a predetermined coverage amount of fragments of the one or more target polynucleotides to form a population containing overlapping first-sized fragments of the one or more target polynucleotides; forming a number of separate mixtures from the population of first-sized fragments; fragmenting the first-sized fragments in the separate mixtures to form populations of second-sized fragments; incorporating oligonucleotide tags into the second-sized fragments in the separate mixtures to produce tagged second-sized fragments, whereby an oligonucleotide tag incorporated into a second-sized fragment in a separate mixture identifies that mixture; obtaining sequence reads by sequencing at least a portion of a plurality of the tagged second-sized fragments from at least some of the mixtures; and obtaining complete or partial nucleotide sequences of the one or more target polynucleotides by assembling at least a portion of the sequence reads, wherein the assembling comprises determining that second-sized fragment sequences comprising the same oligonucleotide tag have the same mixture of origin.