Patent ID: 7052846

Claim:
A kit for genotyping, comprising a plurality of amplification primer pairs, wherein at least one of the primers has the formula: T ms -L-X wherein, T ms is an organic group detectable by mass spectrometry, comprising carbon, at least one of hydrogen and fluoride, and optional atoms selected from oxygen, nitrogen, sulfur, phosphorus and iodine; L is an organic group which allows a T ms -containing moiety to be cleaved from the remainder of the compound, wherein the T ms -containing moiety comprises a functional group which supports a single ionized charge state when the compound is subjected to mass spectrometry and is selected from tertiary amine, quaternary amine and organic acid; and X is a nucleic acid fragment wherein L is conjugated to X at a location other than the 3′ end of X; and each primer pair associates with a different locus; the compound also having the formula: wherein G is (CH 2 ) 1-6 wherein a hydrogen on one and only one of the CH 2 groups is replaced with —(CH 2 ) c -Amide-T 4 ; T 2 and T 4 are organic moieties of the formula C 1-25 N 0-9 O 0-9 H α F β wherein the sum of α and β is sufficient to satisfy the otherwise unsatisfied valencies of the C, N, and O atoms; R 1 is hydrogen or C 1-10 alkyl; c is an integer ranging from 0 to 4; and n is an integer ranging from 1 to 50 such that when n is greater than 1, then G, c, Amide, R 1 and T 4 are independently selected.