Patent ID: 8900814

Claim:
A mutant reverse transcriptase comprising: a DNA interaction region having a substitution of an amino acid residue corresponding to at least one of an aspartate residue at position 124 and a glutamate residue at position 286, such that either the aspartate residue at position 124, the glutamate residue at osition 286 or both the as sartate residue at position 124 and the glutamate residue at position 286 are substituted, in a DNA interaction region of a wild-type reverse transcriptase wherein the substitution or substitutions are that of a positively-charged amino acid residue or a nonpolar amino acid residue for the original negatively-charged amino acid residue or residues, and such that the mutant reverse transcriptase has a positive effective charge larger than that of the DNA interaction region in the wild-type reverse transcriptase, wherein the wild-type reverse transcriptase comprises an amino acid sequence corresponding to SEQ ID NO: 2, and wherein the mutant reverse transcriptase exhibits a reverse transcriptase activity.