Patent ID: 8361536

Claim:
A method for fixing an antibody on the surface of a medical instrument, characterized in that the method includes {circle around (1)} pre-treating the instrument surface, {circle around (2)} preparing holes, {circle around (3)} post-treating the instrument surface and {circle around (4)} fixing the antibody; first, preparing a multicrystal phase structure which has the same size holes in the surface of the instrument by chemical corrosion, electrochemical corrosion, anodic oxidation, micro-arc oxidation, or micro-arc nitridation; and then immersing the bare metal scaffold which has holes in the surface into a buffer solution containing the antibody, adjusting the pH value of the antibody buffer solution, fixing the antibody on the surface of the instrument by the attraction between positive and negative charge and hole effect; and finally, confirming the effectiveness of the fixed antibody by artificial simulation hemodynamics and detecting method of antibody activity on the scaffold surface.