Patent ID: 8372602

Claim:
A method of producing a polypeptide comprising: culturing a cell comprising an expression vector, wherein the expression vector comprises the following operably linked elements: a transcription promoter; a DNA construct encoding a polypeptide, wherein the polypeptide comprises: a DNA segment encoding a polypeptide consisting of an amino acid sequence as shown in SEQ ID NO:2 from amino acid number 20 (Cys) to amino acid number 538 (Ser), wherein the polypeptide further comprises a WSXWS motif as shown in SEQ ID NO:3, and wherein a Trp residue in the WSXWS motif (SEQ ID NO:3) is mannosylated; a transcription terminator, and wherein the promoter is operably linked to the DNA construct, and the DNA construct is operably linked to the transcription terminator, wherein the cell expresses a polypeptide encoded by the DNA construct; and isolating the polypeptide produced by the cell.