Patent ID: 7494789

Claim:
A method for producing a sense RNA molecule, comprising: providing a single stranded cDNA molecule having 5′ and 3′ ends; attaching an oligodeoxynucleotide tail to the 3′ end of said single stranded cDNA molecule; providing a double stranded RNA polymerase promoter having a sense strand and antisense strand, wherein the sense strand comprises a single stranded 3′ overhang comprising a sequence complementary to said oligodeoxynucleotide tail; annealing said double stranded RNA polymerase promoter to said oligodeoxynucleotide tail by complementary base pairing with said 3′ overhang sequence; ligating the 5′ end of the antisense strand of said double stranded RNA polymerase promoter to the 3′ end of said oligodeoxynucleotide tail; and initiating RNA transcription using an RNA polymerase which recognizes said double stranded promoter, thus producing a sense RNA molecule (sRNA).