Patent ID: 8168412

Claim:
A method for producing an optically-active amine compound in a reaction system comprising at least three enzymes and a ketone compound, wherein said enzymes are: (A) a transaminase which uses an α-amino acid as an amino-group donor and which acts on the ketone compound to convert the ketone compound into the optically-active amine compound, and to convert the α-amino acid into an α-keto acid; (B) an α-keto acid reductase, which uses a reduced β-nicotinamide adenine dinucleotide (NADH) or a reduced β-nicotinamide adenine dinucleotide phosphate (NADPH) as a coenzyme and which reduces, to an α-hydroxy acid, the α-keto acid produced from reaction with the transaminase of (A) and which does not act on the ketone compound; and (C) an enzyme that converts oxidized β-nicotinamide adenine dinucleotide (NAD+) produced from the NADH resulting from action of the α-keto acid reductase of (B) to NADH, or that converts oxidized β-nicotinamide adenine dinucleotide phosphate (NADP+) produced from the NADPH resulting from the action of the α-keto acid reductase (B) into NADPH; to convert the ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point.