Patent ID: 7816144

Claim:
A method of analyzing an enoxaparin preparation, comprising: providing an isolated hexasaccharide fraction from a size fractioned enoxaparin preparation; analyzing the hexasaccharide fraction using strong anion exchange high performance liquid chromatography (SAX-HPLC) to determine if one or more chains shown in the following table is present: Identification Hexasaccharide A ΔU 2S H NS6S I 2S H NS6S GH NS6S B ΔU 2S H NS6S I 2S H NS6S I 2S (60-65%) ΔU 2S H NS6S I 2S H NS6S GH NS6S (35-40%) C ΔU 2S H NS6S I 2S H NS6S I 2S H NS (1,6) (56%) ΔU 2S H NS6S I 2S H NS6S I 2S Man NS (1,6) (44%) D ΔU 2S H NS6S I 2S H NS6S I 2S H NS6S E ΔU 2S H NS6S I 2S H NS6S I 2S Man NS6S and if one or more of said chains is present, processing said preparation, wherein processing includes one or more of selecting, accepting, processing into drug product, shipping, formulating, labeling, packaging or selling said preparation, thus analyzing the enoxaparin preparation.