Patent ID: 8883492

Claim:
A cell culture apparatus for culturing cells using a culture medium, comprising: a plurality of flow channels for delivering the culture medium and the cells; a drive base arranged within a first space; a culture vessel connected to the drive base through the flow channels; a holding member for holding the culture vessel; a refrigerating unit which is formed below the drive base to form a refrigerating space, the refrigerating unit configured to store the culture medium and the cells; an intermediate chamber for separating the first space and the refrigerating space, wherein a temperature of the intermediate chamber is lower than a temperature of the first space, and higher than a temperature of the refrigerating space; and a detachable flow channel holding member that holds the flow channels connecting the refrigerating unit with the drive base, and connects the first space, the intermediate chamber, and the refrigerating space, wherein the detachable flow channel holding member is a seal for spatially separating the first space, the refrigerating space, and the intermediate chamber.