Patent ID: 8877495

Claim:
A method of preparing a therapeutic composition for transiently reconstituting hematopoiesis in a human, comprising: a) culturing a starting cell population including enriched human hematopoietic stem cells ex vivo in a culture medium comprising a cytokine and growth factor mixture to produce a final cell population comprising at least 50% expanded myeloid progenitor cells, wherein the expanded myeloid progenitor cells are CD34 + CD90 neg and comprise common myeloid progenitor cells, granulocyte/macrophage progenitor cells, and megakaryocyte/erythroid progenitor cells, and wherein the starting cell population is obtained from multiple, MHC-mismatched donors; and b) resuspending said expanded myeloid progenitor cells in a pharmaceutically acceptable medium suitable for intravenous administration to a human.