Patent ID: 8790883

Claim:
A method for identifying a polypeptide that inhibits the NFκB signaling pathway comprising: a) contacting a cell that contains an NFκB signaling pathway with a candidate polypeptide, wherein said candidate polypeptide comprises (i) a candidate sequence of amino acid residues to be evaluated for its ability to inhibit oligomerization between NF-κB essential modulator (NEMO, IκB kinase-γ/IKK-γ) subunits, (ii) a linker comprising the amino acid residues of SEQ ID NO: 40 or SEQ ID NO: 41, and (iii) a sequence of amino acid residues that permits a cell to internalize the candidate polypeptide, b) detecting NF-κB signaling pathway activity in said cell, c) comparing said NF-κB signaling pathway activity with that in a control cell that was not contacted with the candidate polypeptide, and d) selecting a polypeptide that inhibits NF-κB signaling pathway activity compared to that in the control cell thereby identifying a polypeptide that inhibits the NF-κB signaling pathway.