Patent ID: 8389795

Claim:
A transgenic mouse comprising: (a) a first transgene comprising (i) a regulatory gene encoding a regulatory protein, and (ii) a transcription terminator at which site transcription terminates, said transcription terminator situated 3′ to said regulatory gene, wherein said first transgene has integrated into an endogenous gene of said mouse such that said endogenous gene is mutated and said regulatory gene is positioned for expression under control of the promoter of said endogenous gene, said promoter being operably linked to said regulatory gene upon integration of said transgene into said endogenous gene, and said first transgene mutagenizes said endogenous gene; and (b) a second transgene comprising a gene operably linked to regulatory sequence regulated by said regulatory protein, wherein said second transgene is integrated into the genome of said mouse, wherein said regulatory protein modulates expression of said gene operably linked to said regulatory sequence.