Patent ID: 7432048

Claim:
A method for cleaving multiple oligonucleotides comprising: a) providing: i. a plurality of first oligonucleotides attached to a single solid support; ii. a second oligonucleotide attached to the same solid support as said plurality of first oligonucleotides; iii. a target nucleic acid, wherein said target nucleic acid comprises a first region and a second region, said second region downstream of and contiguous to said first region, wherein at least a portion of each of said first oligonucleotides is completely complementary to said first region of said target nucleic acid, and wherein said second oligonucleotide comprises a 3′ portion and a 5′ portion, wherein said 5′ portion is completely complementary to said second region of said target nucleic acid; and iv. a cleavage agent comprising a FEN-1 endonuclease from an archaebacterial species; and b) exposing said solid support to said cleavage agent under conditions such that: i. a first invasive cleavage structure is formed, said first invasive cleavage structure comprising a first one of said plurality of first oligonucleotides, said second oligonucleotide, and said target nucleic acid; ii. said cleavage agent cleaves said first one of said plurality of first oligonucleotides in said first invasive cleavage structure to produce a first cleavage fragment; iii. after cleavage of said first one of said plurality of first oligonucleotides, a second invasive cleavage structure is formed, said second invasive cleavage structure comprising a second one of said plurality of first oligonucleotides and the same second oligonucleotide as was used in said first invasive cleavage structure; iv. said cleavage agent cleaves said first oligonucleotide in said second cleavage structure to produce a second cleavage fragment.