Patent ID: 7915052

Claim:
An immunoassay method for determining presence of bovine blood in a sample, the method comprising the steps of: combining the sample with a first monoclonal antibody having an affinity for a thermostable bovine blood antigen, wherein the monoclonal antibody is selected from the group consisting of 1B4, 1H9, 2B11, 3D6, 6E1, 6F10, 6G12 and 7F6 produced by hybridoma cell lines deposited as ATCC Nos. PTA-9870, PTA-9869, PTA-9868, PTA-9867, PTA-9982, PTA-9983, PTA-9985 and PTA-9984, respectively; and detecting the formation of a complex between the monoclonal antibody and the bovine blood antigen, thereby detecting the presence of bovine blood in the sample by detecting a label that is attached to (a) the monoclonal antibody; (b) a second monoclonal antibody that is also selected from the group consisting of 1B4, 1H9, 2B11, 3D6, 6E1, 6F10, 6G12 and 7F6; or to (c) a secondary antibody having affinity for the first monoclonal antibody.