Patent ID: 7241582

Claim:
A vertical immunodiffusion enzyme assay (VIDEA) method, said method comprising a) providing a diagnostic device comprising at least one surface coated, by a method comprising i) providing a solution containing one or more PRRS virus proteins and antigens from PRRS virus infected cells, said proteins and antigens having been extracted or eluted from said cells by use of a detergent containing solution; and ii) contacting said solution with a surface for a period of time to allow said proteins and antigens to adsorb to said surface; and said surface having been overlaid with a layer of agar or agarose on said at least one surface; b) contacting the surface of the agar or agarose with a porous material comprising an antibody containing sample derived from a subject; c) incubating said device for a period sufficient to allow diffusion of material from said sample to said one or more surface, said period optionally occurring after removal of the porous material; and d) detecting the presence of antibodies at the surface of the device after removal of the agar or agarose.