Patent ID: 8846767

Claim:
A method of reducing a number of viable microorganisms in a flow path of a microfluidic device, comprising: a) mixing an antimicrobially active agent including diacetone alcohol between about 10% and about 70% by volume and an inactive agent comprising water between about 30% and about 90% to produce an aqueous miscible antimicrobial composition, wherein said diacetone alcohol is the only antimicrobially active agent effective for reducing said number of viable microorganisms in said microfluidic device by contact with said aqueous miscible antimicrobial composition; b) flowing an amount of said aqueous miscible antimicrobial composition within said flow path of said microfluidic device; c) contacting said number of viable microorganisms in said flow path of said microfluidic device with said aqueous miscible antimicrobial composition; and d) reducing said number of viable microorganisms in said flow path of said microfluidic device by contact of said aqueous miscible antimicrobial composition with said viable microorganisms.