Patent ID: 8506781

Claim:
A method for separating a mixture of proteins by electrophoresis, said method comprising (i) loading a gel with a sample of said mixture wherein said gel comprises crosslinked polyacrylamide in a buffer solution comprising triethanolamine, said gel either: having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane, and having about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane; or having about 0.03 mM to about 0.3 mM of tris(hydroxymethyl)aminomethane, and being devoid of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane; or being devoid of tris(hydroxymethyl)aminomethane, and having about 0.03 mM to about 0.3 mM of bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane, and (ii) imposing a voltage across said gel to cause said proteins to migrate through said gel at differential rates until separated into bands within said gel, said gel having been cast and stored for at least three days prior to steps (i) and (ii).