Patent ID: 7736849

Claim:
A method of detecting human cytomegalovirus in an individual consisting of: (a) isolating a nucleic acid from a sample from the individual; (b) providing an amplification reagent and a primer pair selected from the group consisting of an oligonucleotide consisting of, (i) a nucleic acid sequence consisting of at least fifteen continuous bases selected from the nucleic acid sequence of SEQ ID No: 1, SEQ ID No: 2, SEQ ID No: 3, SEQ ID No: 4, SEQ ID No: 5, SEQ ID No: 6 and SEQ ID No: 12, and (ii) a nucleic acid sequence fully complementary to the nucleic acid sequence of (i), wherein the primer pair is SEQ ID NOs: 5 and 6 or SEQ ID NOs: 12 and 6; (c) amplifying a target fragment corresponding to said primer pair; and (d) identifying the amplified fragment by gel electrophoresis followed by staining with ethidium bromide or by an oligonucleotide consisting of (iii) a nucleic acid sequence consisting of at least ten continuous bases selected from the nucleic acid sequence of SEQ ID No: 7, SEQ ID No: 8 and SEQ ID No: 11, and (iv) a nucleic acid sequence fully complementary to the nucleic acid sequence of (iii), wherein the oligonucleotide is used as a probe.