Patent ID: 8389685

Claim:
A plasmid vector comprising DNA which encodes a plasmid replication protein, wherein the amino acid sequence of said replication protein comprises the sequence of SEQ ID NO:2 except that, as compared to SEQ ID NO:2, the amino acid sequence of said replication protein comprises one or more substitutions selected from the group consisting of (a) Ala, Gly, Thr, Arg, Glu, Asn, or Gln at SEQ ID NO:2 amino acid residue position 48, (b) Gly, Ser, Thr, Cys or Val at SEQ ID NO:2 amino acid residue position 262, (c) Asn at SEQ ID NO:2 amino acid residue position 149, and (d) Glu at SEQ ID NO:2 amino acid residue position 198.