Patent ID: 8758999

Claim:
A method of amplifying an archaea thermosome polynucleotide, if present, from at least one archaea organism comprising: a) providing a sample comprising at least one archaea thermosome polynucleotide target; b) adding a primer set for amplifying an archaea thermosome polynucleotide wherein the primer set comprises: i) one or more upstream primers comprising a polynucleotide comprising at least 10 contiguous nucleotides of: GGX 1u CCX 2u X 3u X 4u X 5u GGX 6u X 7u TX 8u GAX 9u AAX 10u ATG (SEQ ID NO:1); and one or more downstream primers comprising a polynucleotide comprising at least 10 contiguous nucleotides of: GCX 1d AX 2d X 3d TCX 4d TCX 5d ATX 6d CCX 7d TTX 8d TG (SEQ ID NO:2); or ii) one or more upstream primers comprising a polyucleotide comprising at least 10 contiguous nucleotides of a sequence reverse complementary to: GGX 1u CCX 2u X 3u X 4u X 5u GGX 6u X 7u TX 8u GAX 9u AAX 10u ATG (SEQ ID NO:1); and one or more downstream primers comprising a polynucleotide comprising at least 10 contiguous nucleotides of a sequence reverse complementary to: GCX 1d AX 2d X 3d TCX 4d TCX 5d ATX 6d CCX 7d TTX 8d TG(SEQ ID NO:2) wherein X 1u is I or C; X 2u is I or G; X 3u is A or C; X 4u is A or G; X 5u is A or G; X 6u is I or C; X 7u is I or A; X 8u is I or G; X 9u is C or T; X 10u is A or G; X 1d is I or C; X 2d is I or T; X 3d is I or G; X 4d is A or G; X 5d is I or G; X 6d is I or G; X 7d is C or T; and X 8d is C or T; and wherein the polynucleotide corresponds to a portion of an archaea thermosome polynucleotide; c) incubating the sample under conditions and with reagents for DNA amplification: wherein each primer is annealed to the thermosome polynucleotide target at a position enabling amplification of the thermosome polynucleotide target and a DNA polymerase amplifies the thermosome polynucleotide target.