Patent ID: 8628933

Claim:
A kit for a homogenous detection method, comprising (a) a first conjugate molecule comprising an analyte-specific binding partner R1 and possessing specific binding sites for a specific binding partner X, wherein R1 is capable of binding to an analyte or to an analyte-specific binding partner R2; (b) a second conjugate molecule comprising an analyte-specific binding partner R2 and possessing specific binding sites for a specific binding partner Y, wherein R2 is capable of binding to the analyte or to R1; (c) the specific binding partner X, which is associated with a first component of a signal-generating system; and (d) the specific binding partner Y, which is associated with a second component of the signal-generating system; wherein at least one of the conjugates comprising R1 or R2 possesses more than one binding site for the respective specific binding partner X or Y; wherein the first and second components of the signal-generating system enter into a detectable interaction when spatially close to each other and the detectable interaction comprises a direct energy transfer between the components; and wherein (i) both R1 and R2 are capable of binding specifically to the analyte, or (ii) only R1 is capable of binding specifically to the analyte and R2 can compete with the analyte in binding to R1.