Patent ID: 8580097

Claim:
A method for separating a complex, the method comprising: (1) introducing in a capillary, between a leading buffer zone and a trailing buffer zone, wherein neither the leading buffer nor the trailing buffer comprises an ion selected from a 2-(N-morpholino)ethane sulfonate ion and a glutamate ion: (a) a blood-derived sample suspected of containing an analyte, (b) one or more mobility-changing complex forming substances (CFS) and (c) one or more labeled CFSs; (2) contacting the analyte, the one or more mobility-changing CFSs, and the one or more labeled CFSs, wherein a complex is formed comprising the analyte, at least one of the one or more mobility-changing CFSs, and at least one of the one or more labeled CFCs; and (3) separating the complex from the labeled CFS not part of said complex and coexisting substances in the blood-derived sample by performing isotachophoresis in the presence of a 2-(N-morpholino)ethane sulfonate ion and/or a glutamate ion between the leading buffer zone and the trailing buffer zone.