Patent ID: 8470580

Claim:
A nucleic acid construct comprising a cassette sequence disposed between a first recombination site and a second recombination site, said cassette sequence comprising a first nucleotide sequence encoding a first portion of an antidote protein to a poison protein wherein said first portion of said antidote protein is inactive and a site for the integration of a DNA insert, said antidote protein being reconstituted as functional antidote protein to the poison protein following insertion of a second nucleotide sequence encoding a second portion of said antidote protein into the cassette, wherein the antidote and poison proteins are selected from the group consisting of the following coupled proteins: CcdA/CcdB protein, Kis/Kid protein, Phd/Doc protein, RelB/RelE protein, PasB/PasA protein, PasC/PasA protein and mazE/mazF protein.