Patent ID: 8765438

Claim:
An isolated polypeptide having xylanase activity and comprising an amino acid sequence, said amino acid sequence having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 1, and which polypeptide has i) one or two amino acid substitutions in a position selected from the group consisting of: 12 and 13; and ii) one or more further amino acid substitutions in a position selected from the group consisting of: 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175, and 179, wherein said positions are determined as the position corresponding to the position of B. subtilis xylanase sequence shown as SEQ ID NO: 1 by alignment; wherein the amino acid modification in position 12 is an amino acid substitution to any one different amino acid residue selected from the group consisting of: isoleucine, alanine, leucine, asparagine, lysine, aspartic acid, methionine, cysteine, glutamic acid, threonine, glutamine, tryptophan, valine, proline, serine, tyrosine, arginine, and histidine; and wherein the amino acid substitution in position 13 is an amino acid substitution to any one different amino acid residue selected from the group consisting of: alanine, leucine, asparagine, lysine, aspartic acid, methionine, cysteine, glutamic acid, threonine, glutamine, tryptophan, valine, proline, serine, tyrosine, arginine, and histidine.