Patent ID: 8383338

Claim:
A method for uniform enrichment of a population of nucleic acid molecules in a sample, comprising: a) providing a sample of nucleic acid molecules comprising a plurality of target nucleic acid sequences, b) hybridizing the sample to nucleic acid probes complementary to the target nucleic acid sequences under hybridization conditions, wherein at least one of the target nucleic acid sequences exhibits enrichment bias for a complementary probe, and wherein the frequency of individual nucleic acid probe sequences is increased for a target nucleic acid sequence having below-average enrichment and decreased for a target nucleic acid sequence having above-average enrichment such that the nucleic acid probes provide uniform hybridization among said plurality of target nucleic acid sequences, and c) separating non-hybridized nucleic acid sequences from hybridized target nucleic acid sequences, thereby uniformly enriching a population of nucleic acid molecules in a sample.