Patent ID: 8076132

Claim:
A method of stabilizing or regressing a tumor in a patient comprising the steps of: collecting monocyte cells from the patient afflicted with the tumor, said tumor selected from the group consisting of gastric carcinoma, epipharyngial carcinoma, sigmoid carcinoma, melanoma, rectal carcinoma, breast carcinoma, pelvic carcinoma, endometrial carcinoma, and mesothelioma; culturing the monocyte cells with IL-4 and GM-CFS to form immature dendritic cells from the monocyte cells; combining the immature dendritic cells and a leukocyte cultured medium adjuvant to form a composition, said leukocyte cultured medium adjuvant comprising at least three cytokines selected from the group consisting of eotaxin, FGF, G-CSF, GM-CSF, IFNγ, IP10, IL1β, IL1ra, IL2, IL4, IL5, IL6, IL7, IL8, IL9, IL10, IL12, IL13, IL15, IL17, MCP1, MIP1α, MIP1β, PDGFbb, RANTES, TNFα, and VEGF; and administering intratumorally a therapeutically effective amount of the composition to the patient.