Patent ID: 8658175

Claim:
A monoclonal antibody or a binding fragment thereof which: (a) specifically binds to human EGFR; (b) competes for binding to human EGFR with an antibody comprising a V H sequence of SEQ ID NO:1 and a V L sequence of SEQ ID NO:2; (c) comprises six CDRs that altogether have up to seven amino acid substitutions, selected from one or more of Tables 3 to 14-3, as compared to the CDRs of SEQ ID NO:3 (CDR-H1), SEQ ID NO:4 (CDR-H2), SEQ ID NO:5 (CDR-H3), SEQ ID NO:6 (CDR-L1), SEQ ID NO:7 (CDR-L2), and SEQ ID NO:8 (CDR-L3), wherein any individual CDR has no more than three amino acid substitutions as compared to the corresponding CDR of SEQ ID NO:3 (CDR-H1), SEQ ID NO:4 (CDR-H2), SEQ ID NO:5 (CDR-H3), SEQ ID NO:6 (CDR-L1), SEQ ID NO:7 (CDR-L2), or SEQ ID NO:8 (CDR-L3); and (d) has a substitution in CDR-L1 selected from G30Y, G30W, G30F, G30T, G30M, G30S, G30C, G30H, G30K, G30Q and G30R (as defined by Kabat numbering) as compared to a CDR-L1 of SEQ ID NO:6.