Patent ID: 8105825

Claim:
A multiple inducible gene regulation system consisting of two orthogonal gene regulation systems, wherein each of said orthogonal gene regulation systems comprises: i) a first polynucleotide encoding a first polypeptide comprising: A) a DNA binding domain, and B) an ecdysone receptor ligand binding domain; and ii) a second polynucleotide encoding a second polypeptide comprising: C) a nuclear receptor ligand binding domain capable of forming a dimer with the ecdysone receptor ligand binding domain of said first polypeptide, and D) a transactivation domain; and iii) a third polynucleotide encoding a third polypeptide, wherein said third polynucleotide is operatively linked to a response element, wherein binding of the DNA binding domain of said first polypeptide to said response element results in expression of said third polypeptide.