Patent ID: 7615213

Claim:
A purified antibody, or antigen-binding fragment thereof, comprising a heavy chain immunoglobulin variable domain and a light chain immunoglobulin variable domain that binds to IL-13 with a K D of less than 10 −7 M as determined by surface plasmon resonance (SPR), wherein the light chain immunoglobulin variable domain comprises the amino acid sequence of: (i) KASESVDNYGKSLMH (SEQ ID NO:19), in CDR1, (ii) RASNLES (SEQ ID NO:20), in CDR2, and (iii) QQSNEDPWT (SEQ ID NO:21), in CDR3; and wherein the heavy chain immunoglobulin variable domain comprises the amino acid sequence of: (i) SYAMS (SEQ ID NO:22), in CDR1, (ii) SISSGGNTYYPDSVKG (SEQ ID NO:23), in CDR2, and (iii) LDGYYFGFAY (SEQ ID NO:24), in CDR3, or an amino acid sequence that differs by fewer than three conservative amino acid substitutions from the amino acid sequence of any of SEQ ID NO:19-24; and wherein the light chain immunoglobulin variable domain, with or without said conservative amino substitutions, contacts IL-13 with residues ASN31 (CDR1), TYR32 (CDR1), LYS34 (CDR1), ARG54 (CDR2), ASN96 (CDR3), ASP98 (CDR3), and TRP100 (CDR3), of SEQ ID NO:11, 12, or 35, and the heavy chain immunoglobulin variable domain, with or without said conservative amino substitutions, contacts IL-13 with residues ILE30, SER31 (CDR1), ALA33 (CDR1), TRP47, SER50 (CDR2), SER52 (CDR2), SER53 (CDR2), TYR58 (CDR2), LEU98 (CDR3), ASP99 (CDR3), GLY100 (CDR3), TYR101 (CDR3), TYR102 (CDR3), and PHE103 (CDR3), of SEQ ID NO:15, 16 or 36.