Patent ID: 8236544

Claim:
An isolated restriction endonuclease selected from the group consisting of: (a) a restriction endonuclease comprising an amino acid sequence having at least 95% sequence identity with amino acids 1 to 381 of SEQ ID NO: 4; (b) a restriction endonuclease encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity with nucleotides 1 to 1143 of SEQ ID NO: 3; and (c) a restriction endonuclease encoded by a polynucleotide that hybridizes under high stringency conditions with nucleotides 1 to 1143 of SEQ ID NO: 3 or its full-length complementary strand, wherein said high stringency conditions are hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washed three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.