Patent ID: 8518669

Claim:
A method for producing a recombinant oxalate decarboxylase, comprising: a step of culturing a recombinant bacterium obtained by transforming a bacterium belonging to the genus Bacillus or to the species Escherichia coli with a recombinant expression plasmid vector comprising an α-amylase promoter having the DNA sequence set forth in any one of SEQ ID Nos. 2 to 4 and an oxalate decarboxylase gene originating in a microorganism that is provided under the control of the α-amylase promoter in a medium having a manganese concentration of 0.1 mM to 1 mM when the bacterium belongs to the genus Bacillus or a manganese concentration of 1 mM to 5 mM when the bacterium belongs to the species Escherichia coli ; and a step of recovering the oxalate decarboxylase thus produced.