Patent ID: 7504089

Claim:
A dye-labeled biomolecule of formula (XV) wherein Z is O, S, or NR 35 wherein R 35 is H or alkyl; R 1 -R 5 are each independently H, alkyl, halo, carboxyl, amino, or SO 3 − Cat + , wherein Cat + is a cation and at least one of R 1 -R 5 is SO 3 − Cat + ; R 6 and R 7 are each H, alkyl, or optionally, together with the group to which they are bonded, form a ring; m and n are each independently integers from 0 to 5; X and Y are each independently CR 19 R 20 , wherein R 19 and R 20 are each independently alkyl, or optionally form a ring together with the carbon atom to which they are bonded; R 8 is a member selected from the group consisting of alkyl, (CH 2 ) r R 25 , (CH 2 ) r R 18 , and R 30 —B, wherein r is an integer from 1 to 50, and R 25 is selected from the group consisting of hydroxyl, thioacetyl, sulfonato, and R 18 is selected from the group consisting of mercapto, amino, haloalkyl, phosporamidityl, N-hydroxy succinimidyl ester, sulfo N-hydroxy succinimidyl ester, isothiocyanato, iodoacetamidyl, and maleimidyl; and R 9 -R 12 and R 14 -R 17 are each independently H, alkyl, halo, amino, sulfonato, R 21 COOH, R 21 OR 22 , R 21 SR 22 , or R 21 COOR 22 wherein R 21 is a bond or alkylene and R 22 is alkyl, or optionally R 11 and R 12 together with the atoms to which they are bonded form an aromatic ring, or optionally R 16 and R 17 together with the atoms to which they are bonded form an aromatic ring; B is a biomolecule; and R 30 is (CH 2 ) r L; wherein r is an integer from 1 to 50, and L is a linking group.