Patent ID: 8883498

Claim:
A method for producing skeletal muscle or skeletal progenitor cells from human pluripotent stem cells comprising: a) culturing human pluripotent stem cells in suspension culture such that an embryoid body is formed; b) culturing the embryoid body of step (a) on gelatin-coated culture dish in a serum-free medium, wherein the embryoid body adheres to the gelatin-coated culture dish; c) dissociating the embryoid body of step (b) such that dissociated cells are obtained; and d) culturing the dissociated cells of step (c) on a type I collagen-coated culture dish, wherein the dissociated cells of step (c) adhere to the type I collagen-coated culture dish, and in a differentiation medium comprising serum, thereby producing skeletal muscle or skeletal muscle progenitor cells.