Patent ID: 7319029

Claim:
A process for producing a thermostable T1 lipase as a fusion protein that lacks a signal peptide, wherein said process comprises culturing an Escherichia coli harboring a recombinant plasmid that comprises a polynucleotide sequence that encodes, as a fusion protein, a thermostable T1 lipase that lacks a signal peptide and glutathione-S-transferase GST, wherein primers of SEQ ID NOS. 2-4 are used for amplification of the open reading frame of the thermostable T1 lipase and its cloning into the recombinant plasmid, wherein the polynucleotide sequence is from a Geobacillus Strain T1, and wherein the Geobacillus Strain T1 is that which is deposited as Geobacillus sp. T1-DSM 17139.