Patent ID: 8263326

Claim:
A method for screening for an agent that modulates NF-κB activity, comprising the steps of: (a) contacting a candidate agent with a polypeptide comprising SEQ ID NO: 18, wherein the polypeptide enhances ubiquitination of phosphorylated IκB, under conditions and for a time sufficient to permit interaction between the polypeptide and candidate agent; and (b) subsequently evaluating the ability of the polypeptide to enhance ubiquitination of phosphorylated IκB, relative to the ability of the polypeptide to enhance ubiquitination of phosphorylated IκB in the absence of said candidate agent using a negative control, wherein said negative control is a mutant polypeptide consisting of SEQ ID NO:18, wherein F box residues 122-168 are deleted; and therefrom identifying an agent that modulates NF-κB activity.