Patent ID: 8758744

Claim:
A prebiotic composition synthesized from an isolated polypeptide having an enzymatic glycosyltransferase activity capable of forming dextrans having α(1→2) linkages from saccharose, α-D-fluoroglucose, paranitrophenyl-α-D glucopyranoside, α-D-glucopyranoside-α-D sorbofuranoside or 4-O-α-D galactopyranosylsucrose, wherein said isolated polypeptide has at least one glucan binding domain and a catalytic activity domain located downstream of the glucan binding domain; and a suitable carrier; wherein said polypeptide is obtained by inserting a nucleic acid sequence comprising: a) two sequences encoding catalytic domains having at least 90% identity with SEQ ID NO: 3; and b) a sequence encoding the glucan binding domain, the latter being located between the two sequences in a) into a host cell; and purifying the polypeptide from a cell extract wherein said polypeptide retains its enzymatic activity.