Patent ID: 7893249

Claim:
A process comprising the steps of: a) synthesizing a nucleic acid molecule comprising one or more ribonucleotides, using a method selected from the group consisting of solid phase phosphoramidite, solution phase phosphoramidite, solid phase H-phosphonate, solution phase H-phosphonate, hybrid phase phosphoramidite, and hybrid phase H-phosphonate-based synthetic methods; b) contacting said nucleic acid molecule from step (a) with aqueous alkylamine, trialkylamine, or alkylamine and trialkylamine, under conditions suitable for the removal of any 2′-amino protecting groups, exocyclic amino (base) protecting groups and/or phosphate protecting groups, which may be individually present or absent, from said molecule; c) contacting reaction mixture having said nucleic acid molecule from step (b) with a polar solvent and trialkylamine•hydrogen fluoride under conditions suitable for the removal of a 2′-OH protecting group; d) loading reaction mixture having said nucleic acid molecule from step (c) onto a chromatography media in a suitable buffer; e) applying a purification gradient using a suitable elution buffer, analyzing the fractions and allowing for the pure fractions to be pooled and desalted.