Patent ID: 7537886

Claim:
A method for the quantitation or detection of one or more target nucleic acid molecules in a sample during nucleic acid synthesis comprising: mixing one or more a target nucleic acid molecules with one or more fluorescently labeled oligonucleotides, wherein said one or more oligonucleotides are labeled with only a single type of fluorescent label and said oligonucleotide undergoes a detectable change in fluorescence upon hybridization of said one or more oligonucleotides to said one or more target nucleic acid molecules; incubating said mixture under conditions sufficient to synthesize one or more nucleic acid molecules complementary to all or a portion of said one or more target nucleic acid molecules, said one or more synthesized nucleic acid molecules comprising said one or more oligonucleotides; and detecting the presence or absence or quantifying the amount of said one or more synthesized nucleic acid molecules by measuring said fluorescent label; wherein said fluorescent label is selected from the group consisting of JOE (2′7′-dimethoxy-4′5′-dichloro-6-carboxyfluorescein), FAM (5-carboxyfluorescein), TAMRA (N,N,N′,N′-tetramethyl-6-carboxyrhodamine), or ROX (6-carboxy-X-rhodamine).