Patent ID: 7893253

Claim:
A method of preparing a reactive sugar, comprising the steps of i. Providing a sample comprising a reducing sugar ii. Providing a solid support (solid) covalently attached to a linker comprising a capture group comprising an —NH 2 group, wherein said linker optionally is attached to said solid support via a spacer iii. Reacting said reducing sugar with said —NH 2 group, thereby obtaining an immobilised sugar, iv. Reacting free —NH 2 groups with a capping agent, wherein the capping agent comprises a reactive group capable of reacting with an —NH 2 group; v. Reducing C═N bonds with a reducing agent; and vi. obtaining an reactive sugar of the structure SugarCH n —NH— linked to a solid support via a linker and optionally a spacer, wherein n is 1 or 2, wherein step iv is performed prior to step v.