Patent ID: 8202523

Claim:
A composition for eliciting antibodies specific for a protein comprising an epitope recognized by antibody 2G12 in a subject, said composition comprising a glycosylated polypeptide comprising at least two N-linked high mannose oligosaccharides that are recognized by antibody 2G12, and a pharmaceutically acceptable excipient, wherein greater than 50% of the N-linked glycans on the polypeptide are the high-mannose oligosaccharides, and wherein the high-mannose oligosaccharides are Man 9 GlcNAc 2 , Man 8 GlcNAc 2 , or a combination thereof, and wherein the polypeptide is expressed by a mutant fungus having disrupted och1, mnn1 and mnn4 genes of Saccharomyces cerevisiae or disrupted homolog genes of och1, mnn1 and mnn4 genes of Saccharomyces cerevisiae , and the polypeptide is isolated from the mutant fungus.