Patent ID: 7465539

Claim:
A method for amplifying a nucleic acid, comprising: preparing a composition comprising a DNA polymerase comprising at least one thermostable DNA polymerase which lacks 3′-exonuclease activity, at least one thermostable DNA polymerase which exhibits 3′-exonuclease activity, and a nucleic acid comprising a sequence to be amplified, wherein the ratio of DNA polymerase activity of the at least one thermostable DNA polymerase which lack 3′-exonuclease activity to DNA polymerase activity of the at least one thermostable DNA polymerase which exhibits 3′-exonuclease activity from about 100:1 up to about 600:1; and subjecting the composition to conditions effective for amplifying the nucleic acid sequence, wherein the nucleic acid sequence to be amplified is a nucleic acid sequence 6 kb or more in length.