Patent ID: 7622281

Claim:
A method of labeling and amplifying a nucleic acid molecule in a plurality of nucleic acid molecules, comprising the steps of: (a) attaching a unique antiprimer P1 to each of a plurality of nucleic acid molecules to be amplified; (b) hybridizing a molecule from step (a) to a solid surface in a plurality of solid surfaces, wherein each of said solid surfaces is attached to a single molecule of primer P1, which is complementary to the unique antiprimer P1 , to form a single captured DNA fragment on each solid surface; (c) extending P1 hybridized in step (b) to form a double stranded fragment, then denaturing the double stranded fragment to form a single stranded captured DNA fragment attached to each solid surface through primer P1; (d) labeling the single stranded captured DNA fragment from step (c) by nucleotide extension; (e) isolating the captured DNA fragment labeled in step (d) by the label; (f) attaching additional primers P1 to the solid surface after isolating the labeled, captured DNA fragment from step (e); and (g) amplifying the captured DNA fragment from step (e) using the additional primers attached in step (f).