Patent ID: 6974678

Claim:
A method of measuring non-binding interactions between a first biomolecule and a second biomolecule to determine an osmotic second virial coefficient, the method comprising: flowing a solution through a micro-fluidic device comprising a solid support contained in a cavity, wherein the first biomolecule is immobilized on a surface of the solid support; injecting a sample comprising the second biomolecule into the flowing solution upstream of the cavity; and measuring the time (retention time, t R ) required for the second biomolecule to emerge from the cavity; wherein the cavity has an internal volume of 100 μl or less, and wherein the retention time (t R ) is a measure of a degree of non-binding interactions between the first biomolecule and the second biomolecule which correlates with the osmotic second virial coefficient for the first biomolecule and second biomolecule pair.