Patent ID: 7425530

Claim:
A method of production of a cyclic L-amino acid of formula (I) or a salt or derivative thereof: wherein said method comprises: (a) expressing a polynucleotide sequence selected from the group consisting of: a polynucleotide sequence comprising SEQ ID NO: 1, a polynucleotide sequence comprising SEQ ID NO: 2, and a polynucleotide sequence comprising SEQ ID NO:5; wherein SEQ ID NOs:1,2 and 5 are generated by mutagenesis and encode ornithine cyclodeaminase enzyme, and wherein the encoded ornithine cyclodeaminase enzyme is produced in a recombinant E.coli strain; (b) reacting an L-diamino acid of formula (II) a salt or derivative of an L-diamino acid of formula (II), or an enantiomeric mixture comprising an L-diamino acid of formula (II) and a corresponding D-diamino acid, the salts or derivatives thereof in variable proportions, with the produced enzyme of step (a); and (c) recovering the cyclic L-amino acid of formula (I) or a salt or derivative thereof in an enantiomeric excess of at least 80%; wherein: R 1 in formula (I) and formula (II) is selected from the group consisting of: a hydrogen atom, a linear or branched alkyl radical having from 1 to 6 carbon atoms and a linear or branched acyl radical having from 1 to 6 carbon atoms; X in formula (I) and formula (II) represents a saturated, or partially or totally unsaturated, linear or branched C 1 -C 9 hydrocarbon chain, optionally comprising in the chain and/or at the end of the chain one or several heteroatoms or heterogroups selected from the group consisting of: O, S, P, and NR 2 ; R 2 represents H or C 1 -C 4 alkyl or acyl group; the hydrocarbon chain is optionally being substituted by one or several identical or different radicals selected from the group consisting of: —R, —O R, —SR, ═ 0 , —C(O)OR, —C(S)OR, —C(O)NR′R″, —C(S)NR′RR″, —CN, —N O 2 , —NR′R″, —NR′C(O)R, —SiOR and -SiOR; R, R′, and R″, are identical or different and represent hydrogen or a linear or branched, saturated, or totally or partially unsaturated, hydrocarbon radical having from 2 to 20 carbon atoms; and R′ and R″ optionally form a ring with the atom carrying them.