Patent ID: 8058506

Claim:
A process of causing a targeted integration of DNA of interest into a plant cell nuclear genome, comprising: (i) providing plant cells with an amplification vector, or a precursor thereof, capable of replication in plant cells, said vector comprising: (a) DNA sequence(s) encoding a geminiviral origin of replication and functional in plant cells, (b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and (c) optionally, further DNA of interest; (ii) providing said plant cells with a replicase gene of the replicase involved in replicating the amplification vector on a non-replicating vector, whereby the replication of said amplification vector in said plant cells is transient; (iii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous recombination; and (iv) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.