Patent ID: 8609365

Claim:
A kit for detecting the presence of a chitin-containing microorganism in a sample suspected of containing the microorganism, comprising in combination: a) an aqueous solution of 0.1% 3-hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid tetrasodium salt in 5% acetic acid in water; b) a solution of 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho [1,2-d]thiazolium bromide in methanol diluted 1:10 in a solution of acetic acid, methanol and deionized water in a ratio of about 10:40:50, respectively; c) a solution of 0.25% sodium dodecyl sulfate in phosphate buffered saline for decolorizing; d) a solution of acetic acid, methanol, and deionized water in the ratio of about 10:40:50, respectively for rinsing agent; and e) a solution of phosphate buffered saline containing 0.05% Tween 20.