Patent ID: 8703498

Claim:
A method of characterizing a mixture of N-glycans, said method comprising steps of: (i) providing a glycan preparation, wherein the glycan preparation includes at least one charged N-glycan and a known quantity of a reference N-glycan, wherein the reference N-glycan is labeled with a labeling agent; (ii) separating the glycan preparation by anion-exchange chromatography to generate a plurality of sample fractions; (iii) separating a first portion of the plurality of sample fractions by at least one secondary chromatographic technique to obtain first separated fractions; (iv) separating a second portion of the plurality of sample fractions by at least one secondary chromatographic technique that differs from the secondary chromatographic technique from (iii) to obtain second separated fractions; (v) quantifying at least one N-glycan relative to the reference N-glycan in at least a portion of the first separated fractions, or in at least a portion of the second separated fractions, or both; and (vi) recording the result from (v) in a quality control record.