Patent ID: 7408026

Claim:
A method for producing a desired protein or domain thereof, which comprises admixing: (I) a first oligopeptide, said first oligopeptide comprising a fragment of said desired protein or domain thereof, and having a C-terminal thioester; and (II) a second oligopeptide, said second oligopeptide comprising a fragment of said desired protein or domain thereof, and having an N-terminal cysteine amino acid residue having an unoxidized sulfhydryl side chain and a free amino group that is capable of forming a β-aminothioester linkage with said C-terminal thioester that rearranges to form an amide bond therein between; wherein said admixing is conducted under conditions sufficient to permit the formation of an amide bond between the C-terminus of said first oligopeptide and the N-terminus of said second oligopeptide.