Patent ID: 7381547

Claim:
A method of detecting Helicobacter pylori in a specimen, the method comprising: (a) performing a multiplex polymerase chain reaction, wherein a plurality of DNA fragments representing a plurality of loci in Helicobacter pylori are amplified by a plurality of forward and reverse primers, wherein for each pair of forward and reverse primer, a primer is internal, the primers selected from the group consisting of AACGC CGTGA GTTCG TCGTA TCG (SEQ ID NO: 6), CATAT AGCCG CTTTT TCTGG TGTCT TTA (SEQ ID NO: 7), CCTCA CGCCA TCAGT CCCAA AAAT (SEQ ID NO: 8), TGATA GGCAA GCAGA CAACG AA (SEQ ID NO: 9), GATGT GTGTG TCAAT ACCAC CAGC (SEQ ID NO: 10), GCAGG ACCCA CGCTA AGATT GT (SEQ ID NO: 11), CAGGT CGCCT TCGCA ATGAG TA (SEQ ID NO: 12), ACGGG AGGCA GCAGT AGGGA ATA (SEQ ID NO: 13), GCCGC GTGGA GGATG AAGGT (SEQ ID NO: 14), TCATG CCTTT ATCGC CTTTT CTCC (SEQ ID NO: 15), GTGGA AAAAG GCGGT ATCGG TCAA (SEQ ID NO: 16), CGATC GCTTT GAGAG GTGCT TTTT (SEQ ID NO: 17), CTAGA GCCTA TGAGT GGGGA ATCTT T (SEQ ID NO: 18), ATCCG TTCCC TTAAC CATAG TGCT (SEQ ID NO: 19), and TGCTA ACTAA CCCCC CGCTA TGGC (SEQ ID NO: 20); and (b) determining that a specimen is positive for Helicobacter pylori if a number of amplified fragments is sufficient to detect Helicobacter pylori in the specimen.