Patent ID: 8124025

Claim:
An article for testing a coagulation process in a whole blood sample including red blood cells, the article comprising: one or more permeable membranes having a first area onto which a whole blood sample is applied and including a first pore, a second area from which a detectable signal is obtained and including a second pore, wherein the first pore has a smaller size rating than the second pore, and wherein the first pore has a size rating effective to exclude a red blood cell included in the whole blood sample; a coagulation initiator provided on the first area in a manner effective to stimulate coagulation of the whole blood sample external to the first pore when the whole blood is applied to the first area; and a substrate associated with the one or more membranes, wherein the substrate reacts with a coagulation cascade component in the blood to produce a detectable signal on the second area; and wherein the substrate is physically separated from the coagulation initiator.