Patent ID: 8652823

Claim:
An isobutanol tolerant Lactobacillus bacterial cell comprising: (a) an engineered isobutanol biosynthetic pathway, wherein the Lactobacillus bacterial cell comprises heterologous genes encoding an acetolactate synthase, an acetohydroxy acid isomeroreductase, an acetohydroxy acid dehydratase or dihydroxyacid dehydratase, a branched-chain keto acid decarboxylase, and a branched-chain alcohol dehydrogenase that perform the following substrate to product conversions: (i) pyruvate to acetolactate catalyzed by the acetolactate synthase, (ii) acetolactate to 2,3-dihydroxyisovalerate catalyzed by the acetohydroxy acid isomeroreductase, (iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate catalyzed by the acetohydroxy acid dehydratase or dihydroxyacid dehydratase, (iv) α-ketoisovalerate to isobutyraldehyde catalyzed by the branched-chain keto acid decarboxylase, and (v) isobutyraldehyde to isobutanol catalyzed by the branched-chain alcohol dehydrogenase; and (b) at least one gene encoding a heterologous fatty acid cistrans isomerase, wherein the isobutanol tolerant Lactobacillus bacterial cell has increased concentration of membrane unsaturated trans fatty acids as compared with that of the corresponding Lactobacillus bacterial cell that lacks the at least one gene encoding a heterologous fatty acid cistrans isomerase.