Patent ID: 7250279

Claim:
An isolated recombinant polypeptide which is: (a) a polypeptide encoded by a polynucleotide comprising the sequence of SEQ ID NO:1; (b) a polypeptide comprising a polypeptide sequence comprising at least 95% identity along the entire length of the polypeptide sequence of SEQ ID NO:2 and which is encoded by a polynucleotide which hybridizes to the complement of SEQ ID NO:1 under stringent conditions comprising overnight incubation at 42° C. in a solution comprising: 50% formamide, 5×SSC (150mM NaCl, 15mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 microgram/ml denatured, sheared salmon sperm DNA; followed by washing the filters in 0.1×SSC at 65° C.; and which possesses pyruvate dehydrogenase phosphatase activity; (c) a polypeptide comprising at least 95% identity along the entire length of the polypeptide sequence of SEQ ID NO:2 and which is encoded by a polynucleotide which hybridizes to the complement of SEQ ID NO:1 under stringent conditions comprising overnight incubation at 42° C. in a solution comprising: 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 microgram/ml denatured, sheared salmon sperm DNA; followed by washing the filters in 0.1×SSC at 65° C. and which possesses pyruvate dehydrogenase phosphatase activity; or (d) the polypeptide sequence of SEQ ID NO:2.