Patent ID: 7556796

Claim:
A method of producing primary tumor material, the tumorigenicity of which depends on a recombinant human gene of interest, comprising the steps of: (a) providing a conditionally tumorigenic mouse cell comprising (i) one or more mutations such that both alleles of an endogenous tumor suppressor gene are absent or nonfunctional, and (ii) a recombinant oncogene operably linked to an inducible promoter, wherein (1) tumorigenicity of the conditionally tumorigenic mouse cell is dependent upon expression of the inducible recombinant oncogene, and (2) the inducible promoter is in the uninduced state; and (b) introducing into the cell a recombinant human gene of interest that functionally complements the recombinant oncogene; (c) introducing the cell of step (b) into an immunocompromised recipient mouse; (d) maintaining the recipient mouse for a suitable tumor latency period, in the absence of an inducer of the inducible promoter; and (e) harvesting primary tumor material from the directed complementation tumor.