Patent ID: 8603491

Claim:
A recombinant DNA comprising: a transcription promoter and, downstream of said promoter and under transcriptional control thereof; a spacer sequence positioned between the transcription promoter and; a cDNA of a genomic RNA of a Salmonidae alphavirus, wherein the spacer sequence is defined by general formula (I) below: 5′ X 1 CTGANGARX 2 B 2 X′ 2 YGAAAX 3 B 3 X′ 3 TH 3′ (I) (SEQ ID NO: 29) in which A, T, G, and C have their usual meaning; H represents C, T or A; R represents A or G; Y represents C or T; N represents A, T, G or C; X 1 represents an oligonucleotide of at least 3 nucleotides, of sequence complementary to that of the 5′ end of the genome of said alphavirus; X 2 represents an oligonucleotide of at least 3 nucleotides, of any sequence; B 2 represents an oligonucleotide of 4 or 5 nucleotides, of any sequence; X′ 2 represents an oligonucleotide complementary to X 2 ; X 3 represents an oligonucleotide of at least 2 nucleotides, of any sequence; B 3 represents an oligonucleotide of 4 to 5 nucleotides, of any sequence; X′ 3 represents an oligonucleotide complementary to X 3 .