Patent ID: 8445234

Claim:
A method of making a VEGF-D polypeptide said method comprising the steps of: culturing a host cell transformed or transfected with a vector comprising a nucleic acid sequence encoding said polypeptide operably associated with a promoter sequence such that the nucleic acid sequence encoding said polypeptide is expressed; and isolating said polypeptide from said host cell or from the growth medium in which said host cell is cultured, wherein said VEGF-D polypeptide is selected from the group consisting of: (a) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO: 5, wherein said fragment and said polypeptide lack amino acids of SEQ ID NO: 5 that are N-terminal to residue 92 of SEQ ID NO: 5 and lack amino acids that are C-terminal to residue 205 of SEQ ID NO: 5; (b) the polypeptide of (a) that comprises at least amino acids 101-196 of SEQ ID NO: 5; or (c) a polypeptide that lacks amino acids of SEQ ID NO: 5 that are N-terminal to residue 93 of SEQ ID NO: 5 and lack amino acids that are C-terminal to residue 201 of SEQ ID NO: 5.