Patent ID: 8691216

Claim:
A method of inducing epithelialization or neodermis formation at a wound of the skin of a subject, comprising: identifying a subject with a wound; providing adipose tissue to a self-contained adipose tissue processing unit comprising: a tissue collecting container configured to receive and fully or partially disaggregate adipose tissue, wherein said tissue collecting container is coupled to a cell collection container wherein said coupling defines a closed system in which said fully or partially disaggregated adipose tissue directed from tissue collecting container to cell collection container is not exposed to the external environment; and wherein said cell collection container, further comprises a cell concentrator that facilitates separation of the cells in a suspension; and an outlet that is structured to permit said concentrated population of cells comprising adipose-derived cells to be removed from said adipose tissue processing unit; obtaining a concentrated population of cells comprising adipose-derived regenerative cells from said self-contained adipose tissue processing unit; contacting said wound with a therapeutically effective amount of the concentrated population of adipose-derived cells comprising regenerative cells, wherein the concentrated population of adipose-derived cells has not been cultured prior to contacting the wound.