Patent ID: 7659072

Claim:
A method for amplifying a target nucleic acid sequence comprising a target nucleic acid: a) hybridizing a Riboprimer to a single stranded DNA template comprising the target nucleic acid sequence, wherein said Riboprimer comprises: i) only ribonucleotides, or ii) only purine ribonucleotides and only pyrimidine nucleotides, wherein at least one of the pyrimidine nucleotides is a pyrimidine 2′-deoxyribonucleotide having a non-canonical substituent, which substituent is neither an H nor an OH, on the 2′-position of the deoxyribose sugar moiety; b) extending the Riboprimer with a DNA polymerase having strand displacement activity; c) cleaving the annealed Riboprimer with an RNase H enzyme such that another Riboprimer hybridizes to the template and repeats primer extension by strand displacement, whereby multiple copies of the complementary sequence of the target sequence are produced; and d) attaching the multiple copies of the complementary sequence of the target sequence onto a solid substrate to make a microarray of the amplified products.