Patent ID: 8283169

Claim:
A method of forming a cell subset enriched dermal filler for a subject in need thereof, the method comprising: a) harvesting adipose fluid and tissue from a subject by liposuctioning said subject, thereby obtaining a harvested sample containing said adipose fluid and tissue; b) pre-testing the harvested sample, comprising determining from a first portion of the harvested sample a relative percentage of CD31, CD144, or CD34 cell subsets in the harvested sample; c) providing a chromatographic column having cell capture layers, wherein each cell capture layer is capable of capturing a cell of the cell subsets and each layer is provided in a cell capturing amount proportional to the determined relative percentage of step b); d) applying the harvested sample or a second portion thereof to the column to capture the cell subsets from the harvested sample or the second portion thereof, thereby providing a bound cell-captured sample portion and an unbound sample portion; e) discarding the unbound sample portion from the column; f) eluting the cell subsets of the bound cell-captured sample from the column, thereby collecting an isolate containing the cell subsets; and g) mixing the cell subsets isolate with adipose tissue thereby forming said cell subset enriched dermal filler.