Patent ID: 8158358

Claim:
A method for gender identification of eagles with probe-based real-time PCR, comprising: (a) providing a DNA of an eagle; (b) performing a probe-based real-time PCR using the DNA as a template, a universal primer pair P2 and P8 as a primer pair and a first probe and a second probe as probes, wherein the 5′ ends of the first probe and the second probe are labeled with a first fluorescent dye and the second fluorescent dye, respectively, the 3′ ends of the first probe and the second probe are both labeled with quencher dyes and the first fluorescent dye is different from the second fluorescent dye, and wherein a sequence of the first probe is a sequence with about 15-38 nucleotides in length of SEQ ID No. 1 or the complementary sequence thereof and a sequence of the second probe is a sequence with about 15-44 nucleotides in length of SEQ ID No. 2 or the complementary sequence thereof; and (c) analyzing a result of the probe-based real-time PCR, wherein if the result is positive for both the first and the second fluorescent dye, the eagle is a female, and if the result is positive for only the first fluorescent dye, the eagle is a male.