Patent ID: 7163702

Claim:
A method for recovering Cucumaria frondosa saponins which comprises: i) extracting (a) freeze dried form of water which had been used to cook Cucumaria frondosa in the industrial processing of Cucumaria frondosa , wherein said water contains Cucumaria frondosa residues, or (b) dried and powdered tissues of Cucumaria frondosa with a mixture of chloroform and methanol under reflux to obtain an organic extract; ii) evaporating the extract; iii) extracting the evaporated extract with ethyl acetate and water one or more times to obtain an aqueous phase; iv) combining the aqueous phase from each ethyl acetate and water extraction; v) performing chromatography of the aqueous phase on Teflon or other non-polar resin and Silica gel columns; and vi) collecting the eluate to obtain Cucumaria frondosa saponins.