Patent ID: 8137937

Claim:
A method for the conversion of a cytosine base in a nucleic acid to an uracil base comprising the steps of a1) binding the nucleic acid to a solid phase capable of binding nucleic acids and incubating the solid phase bound nucleic acid in the presence of sulfite ions thereby deaminating the cytosine base in the nucleic acid, or a2) incubating the nucleic acid in the presence of sulfite ions thereby deaminating the cytosine base in the nucleic acid and binding the nucleic acid to a solid phase, b) optionally washing the solid phase bound nucleic acid, c) contacting the solid phase bound nucleic acid with a solution with an alkaline pH thereby desulfonating the base deaminated in step a1) or a2) in the nucleic acid and converting it to an uracil base and simultaneously eluting the nucleic acid.