Patent Description:
GLP-<NUM> receptor (GLP-1R), is a member of the glucagon receptor subfamily in the G-protein coupled receptor B family (secretin family) of a <NUM>-time transmembrane, and it is widely distributed in pancreas and extra-pancreatic tissues, such as central nervous system, cardiovascular, gastrointestinal tract, lung, kidney, thyroid, skin, lymphocyte, mesenchymal stem cells, etc..

Glucagon-like peptide-<NUM> (GLP-<NUM>) is a natural ligand of the GLP-1R receptor and is a polypeptide compound. It has two forms, namely GLP-<NUM> (<NUM>-<NUM>) and GLP-<NUM> (<NUM>-<NUM>) amide, which differ only by one amino acid sequence. About <NUM>% of the circulating activity of GLP-<NUM> comes from GLP-<NUM> (<NUM>-<NUM>) amide. GLP-<NUM> is expressed by the proglucagon gene. In islet α cells, the main expression product of the proglucagon gene is glucagon, while in the L cells of the intestinal mucosa, the prohormone convertase <NUM> (PC1) cleaves proglucagon to its carboxy-terminal peptide chain sequence, namely GLP-<NUM>. The combination of GLP-<NUM> and GLP-1R receptor can promote the synthesis and secretion of insulin, and also stimulate the proliferation of β cells and inhibit their apoptosis.

GLP-<NUM> exerts the hypoglycemic effect mainly through the following aspects.

GLP-<NUM> can act on islet β cells, promote the transcription of insulin gene, insulin synthesis and secretion, stimulate the proliferation and differentiation of islet β cells, inhibit the apoptosis of islet β cells and increase the number of islet β cells.

In addition, GLP-<NUM> can also act on islet α cells to strongly inhibit the release of glucagon, and act on islet δ cells to promote the secretion of somatostatin, and the somatostatin can also act as a paracrine hormone to participate in the inhibition of glucagon secretion.

Studies have shown that GLP-<NUM> can significantly improve the blood glucose of animal models or patients with type <NUM> diabetes through various mechanisms, wherein, the effect of promoting the regeneration and repair of islet β cells and increasing the number of islet β cells is particularly significant.

GLP-<NUM> has a glucose concentration-dependent hypoglycemic effect, and only when the blood glucose level rises, GLP-<NUM> exerts the hypoglycemic effect, and when the blood glucose level is normal, it will not further decrease.

GLP-<NUM> produces the effect of reducing body weight through various ways, including inhibiting gastrointestinal peristalsis and secretion of gastric juice, suppressing appetite and food intake, and delaying the emptying of gastric contents. GLP-<NUM> can also act on the central nervous system (especially hypothalamus), so that the human body can have a sense of fullness and decreased appetite.

Novo Nordisk recently announced the phase III clinical results of semaglutide (a modified long-acting GLP-<NUM> polypeptide), showing that in all randomized patients, after <NUM> weeks of treatment in obese patients, the mean baseline body weight of the semaglutide <NUM> treatment group decreased by <NUM>% from <NUM>, and the body weight of the placebo group decreased by <NUM>%; semalutide <NUM> group had <NUM>% of patients with ≥<NUM>% weight loss and placebo group had <NUM>% of patients with ≥<NUM>% weight loss.

Just because GLP-<NUM> can significantly improve metabolic diseases by acting on GLP-1R receptor, many domestic and foreign companies have developed various modified or unmodified GLP-<NUM> short-acting (three times a day) or long-acting (once a day or once a week) polypeptide drugs, which include: exenatide, liraglutide, albiglutide, dulaglutide, beinaglutide, lixisenatide, semaglutide, etc..

However, the clinical application of GLP-<NUM> polypeptides and their modifications also faces many problems. Natural GLP-<NUM> is easily degraded by dipeptidyl peptidase IV (DPP-IV) in the body, and its plasma half-life is less than <NUM> minutes. Continuous intravenous infusion or continuous subcutaneous injection is necessary to produce curative effect, which greatly limits the clinical application of GLP-<NUM>. Although the modified GLP-<NUM> can prolong the half-life, its oral bioavailability is low, and there are still great challenges in oral administration. Therefore, there is an urgent need to develop small-molecule GLP-1R receptor agonist drugs that can be administered orally.

<CIT> discloses a compound with the following general formula and a candidate drug PF-<NUM> and Ref-<NUM> (compounds 4A-<NUM> and 3A-<NUM> in the original literature, which are used as reference compounds hereinafter).

Surprisingly, compared with PF-<NUM> and Ref-<NUM>, most of the compounds in the present disclosure not only show good activity, but also show better pharmacokinetic properties in vivo, and are more suitable for drug development.

<CIT> discloses the compound with the following general formula and the following compound Ref-<NUM> (embodiment <NUM> in the original literature, which is used as a reference compound hereinafter).

Surprisingly, compared with compound Ref-<NUM>, the compounds of the present disclosure not only have high activity, but also show better pharmacokinetic properties.

The technical purpose of the present disclosure is to provide a class of compounds with GLP-1R receptor agonistic activity.

According to one aspect of the present disclosure, the present disclosure provides a compound represented by the following formula (IV) or a pharmaceutically acceptable salt thereof
<CHM>
wherein,.

Preferably, in the structure of the compound represented by formula (IV) of the present disclosure, the R<NUM> is -F, -Cl, -Br, -CN, -C<NUM>-<NUM> alkyl, -C<NUM>-<NUM> alkoxy, -C<NUM>-<NUM> alkenyl or -C<NUM>-<NUM> alkynyl;.

Furthermore preferably, the compound according to the present disclosure is selected from one of the following compounds:
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>.

According to another aspect of the present disclosure, the present disclosure provides a pharmaceutical composition, comprising a therapeutically effective amount of the above compounds represented by formula (IV) or the pharmaceutically acceptable salt thereof, or any of the above listed compounds or a pharmaceutically acceptable salt thereof, as an active ingredient, and a pharmaceutically acceptable excipient.

According to another aspect of the present disclosure, the present disclosure provides the above compound or the pharmaceutically acceptable salt thereof or the above pharmaceutical composition for use in treating metabolism-related diseases by activating a GLP-1R receptor.

In specific embodiments, the metabolism-related diseases are selected from any one of glucose intolerance, hyperglycemia, dyslipidemia, type <NUM> diabetes (T1D), type <NUM> diabetes (T2D), hypertriglyceridemia, syndrome X, insulin resistance, impaired glucose tolerance (IGT), diabetic dyslipidemia, hyperlipidemia, arteriosclerosis, atherosclerosis, hypertension, obesity, non-alcoholic fatty liver, non-alcoholic steatohepatitis, hepatic fibrosis, cirrhosis and lethargy.

The present disclosure synthesizes a novel class of GLP-1R receptor agonist compounds, which are confirmed by pharmacological experiments to have good agonistic activity on GLP-1R receptor, and therefore can be used for the treatment of GLP-1R receptor-related metabolic diseases. In addition, the compound of the present disclosure also exhibits excellent drug metabolism properties.

Before proceeding with the description, it should be understood that the terms used in this specification and appended claims should not be construed as being limited to ordinary and dictionary meanings, but should be construed in accordance with the meaning and concept corresponding to the technical aspects of the present disclosure on the basis of the principle that allows the inventors to appropriately define the terms for optimum interpretation.

According to the present disclosure, all terms cited herein have the same meanings as understood by those skilled in the art, unless otherwise stated.

As used herein, the term "salt" refers to compounds containing cations and anions that can be produced by protonation of proton-acceptable sites and/or deprotonation of proton-available sites. It is worth noting that protonation of the proton-acceptable site leads to the formation of cationic substances whose charge is balanced by the presence of physiological anions, while deprotonation of the proton-available site leads to the formation of anionic substances whose charge is balanced by the presence of physiological cations.

The term "pharmaceutically acceptable salt" means that the salt is pharmaceutically acceptable. Examples of pharmaceutically acceptable salts include, but are not limited to: (<NUM>) acid addition salts, formed with inorganic acids, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, etc.; or formed with organic acids, such as glycolic acid, pyruvic acid, lactic acid, malonic acid, malic acid, maleic acid, fumaric acid, tartaric acid, citric acid, <NUM>-(<NUM>-hydroxybenzoyl)benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, <NUM>,<NUM>-ethanedisulfonic acid, <NUM>-hydroxyethanesulfonic acid, benzenesulfonic acid, <NUM>-chlorobenzenesulfonic acid, <NUM>-naphthalenesulfonic acid, p-toluenesulfonic acid, camphoric acid, dodecylsulfuric acid, gluconic acid, glutamic acid, salicylic acid, cis-muconic acid, etc.; or (<NUM>) base addition salts, formed with any one of the conjugate bases of the above inorganic acids, wherein the conjugate base includes a cation component selected from Na+, K+, Mg<NUM>+, Ca<NUM>+, NHxR<NUM>-x+, wherein NHxR<NUM>-x+ (R is C<NUM>-<NUM> alkyl, and subscript x is an integer selected from <NUM>, <NUM>, <NUM>, <NUM> or <NUM>) represents the cation in the quaternary ammonium salt. It should be understood that all salts involving pharmaceutically acceptable salts include solvent addition form (solvate) or crystalline form (polymorph) as defined herein for the same acid addition salts.

The term "C<NUM>-M alkyl" refers to an alkyl containing <NUM>-M carbon atoms, for example, wherein M is an integer having the following values: <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>. For example, the term "C<NUM>-<NUM> alkyl" refers to an alkyl containing <NUM>-<NUM> carbon atoms. Examples of alkyl include, but are not limited to, lower alkyl including methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, pentyl, isopentyl, neopentyl, hexyl, heptyl and octyl.

The term "C<NUM>-M cycloalkyl" refers to a cycloalkyl containing <NUM>-M carbon atoms, for example, wherein M is an integer with the following values: <NUM>, <NUM>, <NUM>, <NUM>, <NUM>. For example, the term "C<NUM>-<NUM> cycloalkyl" refers to a cycloalkyl containing <NUM>-<NUM> carbon atoms. Examples of cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, etc..

The term "aryl" refers to an aromatic system, which may be a single ring or polyaromatic rings that are fused or linked together such that at least a portion of the fused or linked rings form a conjugated aromatic system. Aryl groups include, but are not limited to: phenyl, naphthyl, tetrahydronaphthyl. Aryl may be optionally substituted, such as aryl or heterocycle that may be substituted by <NUM>-<NUM> groups selected from the group consisting of halogen, -CN, -OH, -NO<NUM>, amino, alkyl, cycloalkyl, alkenyl, alkynyl, alkoxy, aryloxy, substituted alkoxy, alkylcarbonyl, alkylcarboxy, alkylamino, or arylthio.

The term "substituted" means that the reference group may be substituted by one or more additional groups, and the additional groups are individually and independently selected from alkyl, cycloalkyl, aryl, heteroaryl, heteroalicyclic hydrocarbon, hydroxy, alkoxy, alkylthio, arylthio, alkylsulfinyl, arylsulfinyl, alkylsulfonyl, arylsulfonyl, cyano, halogen, carbonyl, thiocarbonyl, nitro, haloalkyl, fluoroalkyl and amino, including monosubstituted and disubstituted amino groups and protected derivatives thereof.

The compound or the pharmaceutically acceptable salt thereof provided by the present disclosure and the pharmaceutical composition comprising the compound provided by the present disclosure may be in various forms, such as tablets, capsules, powders, syrups, solution forms, suspensions and aerosols, and may be present in a suitable solid or liquid carrier or diluent and in a suitable sterile apparatus for injection or infusion.

Various dosage forms of the pharmaceutical composition of the present disclosure can be prepared according to conventional preparation methods in the field of pharmacy. For example, the unit dose of the formulation contains <NUM>-<NUM> of the compound or the pharmaceutically acceptable salt thereof provided by the present disclosure, preferably, the unit dose of the formulation contains <NUM>-<NUM> of the compound.

The compound and the pharmaceutical composition of the present disclosure can be clinically used in mammals, including humans and animals, and can be administered via oral, nasal, dermal, pulmonary or gastrointestinal routes. Oral administration is most preferred. The optimal daily dose is <NUM>-<NUM>/kg body weight in a single dose or <NUM>-<NUM>/kg body weight in divided doses. Regardless of the method of administration, the optimal dose for an individual should depend on the specific treatment. It is common to start with a small dose and gradually increase the dose until the most suitable dose is found.

In the present disclosure, the term "effective amount" may refer to an effective amount of the dose and time period required to achieve the desired effect. The effective amount may vary depending on certain factors, such as the type of disease or condition of the disease at the time of treatment, the configuration of the particular subject organ being administered, the size of the individual patient or the severity of the disease or symptom. The effective amount of a particular compound can be determined empirically by those skilled in the art without excessive experiments.

A typical formulation is prepared by mixing the compound of the present disclosure and a carrier, diluent or excipient. Suitable carrier, diluent or excipient is well known to those skilled in the art and includes substances such as carbohydrates, waxes, water-soluble and/or swellable polymers, hydrophilic or hydrophobic substances, gelatin, oils, solvents, water, etc..

The particular carrier, diluent or excipient employed will depend upon the mode and purpose of use of the compound of the present disclosure. Solvents are generally selected on the basis of solvents considered safe and effective for administration to mammals by those skilled in the art. In general, safe solvents are non-toxic aqueous solvents such as water, and other non-toxic solvents that are soluble or miscible in water. Suitable aqueous solvents include one or more of water, ethanol, propylene glycol, polyethylene glycol (e.g., PEG400, PEG300), etc. The formulation may also include one or more buffers, stabilizers, surfactants, wetting agents, lubricants, emulsifiers, suspensions, preservatives, antioxidants, opacifiers, glidants, processing aids, colorants, sweeteners, spices, flavorings or other known additives to enable the drug to be manufactured or used in an acceptable form.

When the compound of the present disclosure is used in combination with at least one other drug, the two or more drugs can be used separately or in combination, preferably in the form of a pharmaceutical composition. The compound or the pharmaceutical composition of the present disclosure can be administered separately or in combination in any known form of oral administration, intravenous injection, rectal administration, vaginal administration, transdermal absorption, other local or systemic administration form to the subject.

The pharmaceutical composition may also include one or more buffers, stabilizers, surfactants, wetting agents, lubricants, emulsifiers, suspensions, preservatives, antioxidants, opacifiers, glidants, processing aids, colorants, sweeteners, spices, flavorings or other known additives to enable the pharmaceutical composition to be manufactured or used in an acceptable form.

The drug of the present disclosure is preferably administered orally. Solid dosage forms for oral administration may include capsules, tablets, powder or granule formulations. In solid dosage forms, the compound or the pharmaceutical composition of the present disclosure is mixed with at least one inert excipient, diluent or carrier. Suitable excipients, diluents or carriers include substances such as sodium citrate or dicalcium phosphate, or starch, lactose, sucrose, mannitol, silicic acid, etc.; binders such as carboxymethylcellulose, alginate, gelatin, polyvinylpyrrolidone, sucrose, gum arabic, etc.; wetting agents such as glycerin, etc.; disintegrants such as agar, calcium carbonate, potato or tapioca starch, alginic acid, specific complex silicate, sodium carbonate, etc.; solution blockers such as paraffin, etc.; absorption promoters such as quaternary ammonium compounds, etc.; adsorbents such as kaolin, bentonite, etc.; lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycol, sodium dodecyl sulfate, etc. In the case of capsules and tablets, the dosage form may also include a buffer. Similar types of solid compositions may also be used as fillers in soft and hard filled gelatin capsules, using lactose and high molecular weight polyethylene glycols as excipients.

Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups and elixirs. In addition to the compound or the pharmaceutical composition thereof of the present disclosure, the liquid dosage form may contain inert diluents commonly used in the art, such as water or other solvents; solubilizers and emulsifiers such as ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, <NUM>,<NUM>-butanediol, dimethyl formamide; oils (e.g., cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil, sesame oil); glycerin; tetrahydrofurfuryl alcohol; fatty acid esters of polyethylene glycol and dehydrated sorbitol; or mixtures of several of these substances, etc..

In addition to these inert diluents, the composition may also include excipients, such as one or more of wetting agents, emulsifiers, suspensions, sweeteners, flavorings and spices.

In the case of suspensions, in addition to the compound or the pharmaceutically acceptable salt thereof provided by the present disclosure or the pharmaceutical composition containing the same of the present disclosure, suspensions may further contain carriers such as suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol, dehydrated sorbitol ester, microcrystalline cellulose, AlO(OH), bentonite, agar and tragacanth gum, or mixtures of several of these substances, etc..

The compound or the pharmaceutically acceptable salt thereof provided by the present disclosure or the pharmaceutical composition containing the same of the present disclosure may be administered in other topical delivery forms, including creams, powders, sprays and inhalants. The drug may be mixed under sterile conditions with a pharmaceutically acceptable excipient, diluent or carrier and any one preservative, buffer or propellant as desired. Ophthalmic formulations, ophthalmic ointments, powders and solutions are also intended to be within the scope of the present disclosure.

In addition, the present disclosure also covers kits (such as pharmaceutical packaging). Provided kits may contain the pharmaceutical composition or the compound described herein and a container (e.g., vial, ampoule, bottle, syringe and/or sub-package or other suitable container). In some embodiments, provided kits may optionally further contain a second container, comprising a pharmaceutically acceptable excipient for diluting or suspending the pharmaceutical composition or the compound described herein. In some embodiments, the pharmaceutical compositions or the compounds described herein disposed in a first container and a second container are combined to form a unit dosage form.

In some embodiments, the kits described herein further contain instructions for using the compound or the pharmaceutical composition contained in the kit. The kits described herein may also include information required by regulatory agencies, such as the U. Food and Drug Administration (FDA). In some embodiments, the information contained in the kit is prescription information. In some embodiments, the kits and instructions are provided for use in treating a proliferative disease in a subject in need thereof and/or preventing a proliferative disease in a subject in need thereof. The kits described herein may contain one or more additional pharmaceutical agents as separate compositions.

The present disclosure is described in further detail hereinafter in connection with specific embodiments, but the present disclosure is not limited to the following embodiments, and embodiments are intended to better illustrate certain specific implementations of the present disclosure and are not to be construed as limiting the scope of the present disclosure in any way. The conditions not indicated in the embodiments are conventional conditions. Unless otherwise specified, the reagents and instruments used in the following embodiments are all commercially available products.

The structures of the compounds in the following embodiments are determined by nuclear magnetic resonance (NMR) or/and mass spectrometry (MS). NMR shifts (δ) are given in units of <NUM>-<NUM> (ppm). The determination of NMR uses a Bruker AVANCE-<NUM> nuclear magnetic instrument, and the determination solvent is deuterated dimethyl sulfoxide (DMSO-d<NUM>), deuterated chloroform (CDCl<NUM>), deuterated methanol (CD<NUM>OD), and the internal standard is tetramethylsilane (TMS).

The determination of MS uses a FINNIGAN LCQAd (ESI) mass spectrometer (manufacturer: Thermo, model: Finnigan LCQ advantage MAX).

Column chromatography generally uses Yantai Huanghai silica gel <NUM>-<NUM> mesh silica gel as a carrier.

The temperature of the reaction in the embodiment is room temperature, <NUM>-<NUM>, unless otherwise specified.

The elution system of the column chromatography adopted in the embodiment includes: A: dichloromethane and methanol system, B: n-hexane and ethyl acetate system, C: petroleum ether and ethyl acetate system, D: acetone and petroleum ether system, and the volume ratio of the solvent is adjusted according to the polarity of the compound.

Abbreviations used in experiments: D: deuterium; h: hour; min: minute; EA: ethyl acetate; DMF: N,N-dimethylformamide; mL: milliliter; mmol: millimole; Cs<NUM>CO<NUM>: cesium carbonate; PdCl<NUM>(dtbpf): [<NUM>,<NUM>'-bis(diphenylphosphino)ferrocene]palladium dichloride; Boc: tert-butoxycarbonyl; THF: tetrahydrofuran; DCM: dichloromethane; DIPEA: diisopropylethylamine; TFA: trifluoroacetic acid; TEA: triethylamine; THF: tetrahydrofuran; TBD: <NUM>,<NUM>,<NUM>-triazabicyclo[<NUM>. <NUM>]dec-<NUM>-ene; N: moles per liter.

The following embodiments <NUM>, <NUM>-<NUM>, <NUM>-<NUM>, <NUM>-<NUM> are not according to the invention and are present for illustration purposes only.

Compound <NUM> was prepared using starting material <NUM>-<NUM> according to the following route.

Starting material <NUM>-<NUM> (<NUM>, <NUM> mmol) was dissolved in dioxane (<NUM>), then intermediate <NUM>-<NUM> (<NUM>, <NUM> mmol), Cs<NUM>CO<NUM> (<NUM>, <NUM> mmol), Pd(dppf)Cl<NUM> (<NUM>, <NUM> mmol) were added thereto, heated to <NUM> and reacted overnight. After the reaction was completed, the mixture was cooled to room temperature, extracted by adding <NUM> of water and <NUM> of EA, dried, and the organic phase was concentrated, and subjected to column chromatography to obtain intermediate <NUM>-<NUM> (<NUM>, <NUM>%).

Intermediate <NUM>-<NUM> (<NUM>, <NUM> mmol) was added to methanol (<NUM>), then <NUM>% Pd/C (<NUM>, <NUM>%) was added thereto, and reacted overnight at room temperature. After the reaction was completed, the mixture was filtered, and the filtrate was concentrated to obtain the hydrogenated product <NUM>-<NUM> (<NUM>, <NUM>%). <NUM>H NMR (<NUM>, CDCl<NUM>): <NUM>-<NUM> (m, <NUM>), <NUM> (t, J= <NUM>, <NUM>), <NUM> (t, J= <NUM>, <NUM>), <NUM> (brs, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (brs, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>);.

Intermediate <NUM>-<NUM> (<NUM>, <NUM> mmol), substituted benzyl bromide compound <NUM>-<NUM> (<NUM>, <NUM> mmol) were dissolved in acetonitrile (<NUM>), then potassium carbonate (<NUM>, <NUM> mmol) was added thereto, and reacted at room temperature overnight. After the reaction was completed, the mixture was concentrated, added with <NUM> of water, extracted twice with EA, dried over anhydrous sodium sulfate, and concentrated to obtain a crude product of <NUM>-<NUM> as a white solid (<NUM>, <NUM>%).

<NUM>H NMR (<NUM>, CDCl<NUM>): <NUM> (t, J= <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (brs, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (brs, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

Intermediate <NUM>-<NUM> (<NUM>, <NUM> mmol) was added to DCM (<NUM>), then TFA (<NUM>) was added thereto, and reacted at room temperature for <NUM> hours. After the reaction was completed, the mixture was concentrated, added with EA, and the pH was adjusted to <NUM> with saturated sodium bicarbonate aqueous solution. The phases were separated, and the organic phase was dried and concentrated to obtain intermediate <NUM>-<NUM> (<NUM>), which was directly put into the next reaction without purification.

Intermediate <NUM>-<NUM> (<NUM>, <NUM> mmol), chlorinated compound <NUM>-<NUM> (<NUM>, <NUM> mmol, synthesized with reference to <CIT>) were dissolved in dioxane (<NUM>), and DIPEA (<NUM>, <NUM> mmol) was added thereto, heated to <NUM> and reacted. After the reaction was completed, H<NUM>O (<NUM>) and EA (<NUM>) were added for extraction, and the organic phase was dried and concentrated, and purified by column chromatography to obtain intermediate <NUM>-<NUM> (<NUM>, <NUM>%).

<NUM>H NMR (<NUM>, CDCl<NUM>): <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

Intermediate <NUM>-<NUM> (<NUM>, <NUM> mmol) was added to a mixed solvent of MeCN/THF (<NUM>/<NUM>), then <NUM> N <NUM>,<NUM>,<NUM>-triazabicyclo[<NUM>. <NUM>]dec-<NUM>-ene (TBD) (<NUM>) was added thereto, and the reaction was carried out overnight at room temperature. Additional <NUM>. 97N TBD (<NUM>) was added thereto, and the reaction was continued for <NUM> hours. The mixture was concentrated to remove the solvent, added with water (<NUM>). The pH of the system was adjusted to <NUM>-<NUM> with 1N citric acid aqueous solution, and the mixture was added with EA (<NUM>), extracted to obtain the organic phase, dried and concentrated, purified by column chromatography to obtain compound <NUM> (<NUM>, <NUM>%).

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

According to the similar synthesis method of embodiment <NUM>, the starting material (<NUM>-fluoro-<NUM>-hydroxyphenyl)phenylboronic acid was replaced with <NUM>-<NUM> (<NUM>-hydroxyphenyl) to prepare compound <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

According to the similar synthetic method of embodiment <NUM>, except adopting <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>-fluorobenzene in step <NUM> of embodiment <NUM> instead of compound <NUM>-<NUM> (<NUM>-(bromomethyl)-<NUM>-fluorobenzonitrile), compound <NUM> was prepared in the same method as in embodiment <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

According to the similar synthesis method of embodiments <NUM> and <NUM>, the starting material (<NUM>-fluoro-<NUM>-hydroxyphenyl)phenylboronic acid was replaced with <NUM>-<NUM> (<NUM>-hydroxyphenyl) to prepare compound <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

According to the similar synthesis method of embodiments <NUM> and <NUM>, the starting material (<NUM>-hydroxy-<NUM>-methoxyphenyl)phenylboronic acid was replaced with <NUM>-<NUM> (<NUM>-hydroxyphenyl) to prepare compound <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM> of compound <NUM> was dissolved in <NUM> of methanol, added with <NUM> of Pd/C, and hydrogenated overnight at room temperature, filtered and concentrated to obtain compound <NUM> (<NUM>).

According to the similar synthetic method of embodiment <NUM>, except adopting <NUM>-(bromomethyl)-<NUM>-fluoro-<NUM>-fluorobenzene in step <NUM> of embodiment <NUM> instead of compound <NUM>-<NUM> (<NUM>-(bromomethyl)-<NUM>-fluorobenzonitrile), compound <NUM> was prepared in the same method as in embodiment <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

According to the similar synthetic method of embodiment <NUM>, except adopting <NUM>-(bromomethyl)-<NUM>-chloropyridine in step <NUM> of embodiment <NUM> instead of compound <NUM>-<NUM> (<NUM>-(bromomethyl)-<NUM>-fluorobenzonitrile), compound <NUM> was prepared in the same method as in embodiment <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>). MS m/z (ESI): <NUM> [M+<NUM>].

According to the similar synthetic method of embodiment <NUM>, except adopting <NUM>-chloro-<NUM>-(<NUM>-chloroethyl)-<NUM>-fluorobenzene in step <NUM> of embodiment <NUM> instead of compound <NUM>-<NUM> (<NUM>-(bromomethyl)-<NUM>-fluorobenzonitrile), compound <NUM> was prepared in the same method as in embodiment <NUM>.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (t, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (q, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>).

According to the similar synthesis method of embodiment <NUM>, compound <NUM> was prepared.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>HNMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>HNMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>);.

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (brs, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>H NMR (<NUM>, DMSO-d<NUM>): <NUM> (brs, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM> (d, J= <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

This experiment aims to verify the agonistic activity of the compound of the present disclosure on human GLP-1R receptor.

Compounds were diluted <NUM>-fold starting at <NUM> from DMSO using Bravo for a total of <NUM> points.

The reference compound polypeptide GLP-<NUM> (<NUM>-<NUM>) was diluted <NUM>-fold starting from <NUM> in DMSO using Bravo for a total of <NUM> points.

TopSeal-A was unloaded and read on EnVision.

The specific test data are shown in Table <NUM> below.

Conclusion: The compounds of the present disclosure show good GLP-1R receptor agonistic activity.

The drug concentrations in the plasma of rats were tested at different times after the administration of the compounds by gavage using rats as test animals. The pharmacokinetic behavior of the compounds of the present disclosure in rats was studied and their pharmacokinetic characteristics were evaluated. In each group of embodiments, <NUM> rats with similar body weight were selected and administered orally at a dose of <NUM>/kg for a single administration. Blood was collected from animals at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> time points after administration. The LC-MS/MS analysis method was used to detect the content of the compound in plasma, and the lower limit of quantification of the method was <NUM> ng/mL. Concentration data in plasma was counted using the metabolic kinetic data analysis software WinNonlin <NUM>, and the pharmacokinetic parameters were calculated using the non-compartmental analysis (NCA), as shown in Table <NUM> below.

Experimental drugs: Compounds of the present disclosure and reference compounds.

Drug configuration: A certain amount of drug was taken, added with <NUM>% Klucel LF+<NUM>% Tween <NUM> aqueous solution to prepare a clear solution or a uniform suspension.

Administration: Rats were administered by gavage after overnight fasting at a dose of <NUM>/kg.

Operation: Rats were administered by gavage, and blood was collected from the tail vein before administration and <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> after administration, placed in heparinized sample tubes, centrifuged at <NUM>, <NUM> rpm for <NUM> to separate plasma, stored at -<NUM>, and fed <NUM> hours after administration.

Determination of the content of test compounds in rat plasma after drug administration by gavage: <NUM>µL of plasma samples were thawed at room temperature, added with <NUM>µL of internal standard working solution (<NUM> ng/mL, acetonitrile, tolbutamide), vortexed for about <NUM> and then centrifuged for <NUM> at <NUM> and <NUM> rpm. <NUM>µL of supernatant was mixed with <NUM>µL of <NUM>% acetonitrile in water, and then the sample was injected for LC/MS/MS analysis.

The results of pharmacokinetic parameters are shown in Table <NUM>.

Conclusion: Compared with the reference compounds PF-<NUM>, Ref-<NUM> and Ref-<NUM>, the compounds of the present disclosure have better absorption, higher drug exposure in blood, and have excellent drug metabolism properties.

The drug concentrations in the plasma of cynomolgus monkeys were tested at different times after intravenous injection and oral administration of the compounds using cynomolgus monkeys as test animals. The pharmacokinetic behavior of the compounds of the present disclosure in cynomolgus monkeys was studied and their pharmacokinetic characteristics were evaluated. In each group of embodiments, <NUM> cynomolgus monkeys with similar body weight were selected, administered at a dose of <NUM>/kg by intravenous injection and at a dose of <NUM>/kg by oral administration for a single administration.

Age of cynomolgus monkeys at the time of administration: about <NUM>-<NUM> years old; body weight: <NUM>-<NUM> at the beginning of administration; <NUM> monkeys; sex: male.

Intravenous group: The final concentration of <NUM>/mL was prepared for intravenous administration, and the preparation solvent was <NUM>% DMSO + <NUM>% PEG400 + <NUM>% water, and the preparation was in a clarified solution.

Oral group: The final concentration of <NUM>/mL was prepared for oral administration, and the preparation solvent was <NUM>% DMSO + <NUM>% PEG400 + <NUM>% water, and the preparation was in homogeneous suspension.

Blood was collected from animals at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> time points after administration. The LC-MS/MS analysis method was used to detect the content of the compound in plasma, and the lower limit of quantification of the method was <NUM> ng/mL. Concentration data in plasma was counted using the metabolic kinetic data analysis software WinNonlin <NUM>, and the pharmacokinetic parameters were calculated using the non-compartmental analysis (NCA), as shown in Table <NUM> below.

Determination of the content of test compounds in the plasma of cynomolgus monkeys after drug administration: After blood samples were collected, placed in a marked ice-bath centrifuge tube, and rapidly centrifuged to separate the plasma. Centrifugation conditions: <NUM> rpm, <NUM> minutes, <NUM>, and plasma was stored at -<NUM> or less for testing.

Claim 1:
A compound represented by the following formula (IV) or a pharmaceutically acceptable salt thereof:
<CHM>
wherein,
R<NUM> is halogen, -CN, -OH, deuterium, -C<NUM>-<NUM> alkyl, -C<NUM>-<NUM> alkoxy, -C<NUM>-<NUM> alkenyl, or -C<NUM>-<NUM> alkynyl; the -C<NUM>-<NUM> alkyl, -C<NUM>-<NUM> alkoxy, -C<NUM>-<NUM> alkenyl or -C<NUM>-<NUM> alkynyl is substituted by <NUM>-<NUM> F;
subscript m is an integer of <NUM> or <NUM>;
A is phenyl, or pyridyl;
R<NUM> is halogen, -OH, deuterium, -C<NUM>-<NUM> alkyl or -C<NUM>-<NUM> alkoxy;
subscript n is an integer of <NUM> or <NUM>;
B is
<CHM>
R<NUM> is -C<NUM>-<NUM> alkyl;
subscript o is an integer of <NUM>;
or a compound selected from one of the following compounds, or a pharmaceutically acceptable salt thereof:
<CHM>
<CHM>