Patent Description:
Medical in vitro diagnosis plays a quite important role in today's medical industry, by means of which changes of various biological indicators in body fluid can be qualitatively or quantitatively measured so as to provide advice on disease diagnosis or treatment indicators and the like, for example, the test of glycosylated hemoglobin (HbA1c) in blood is essential for the diagnosis and control of diabetes.

The glycosylated hemoglobin is a binding product of hemoglobin and blood glucose in erythrocytes in human blood, and when the glucose concentration in the blood is high, the content of HbAlc formed by the human body is also relatively high. The average lifetime of the erythrocytes in the human body is <NUM> days, and before death of the cells, the content of HbAlc in the blood remains relatively constant, therefore the glycosylated hemoglobin test can usually reflect the blood glucose control condition of a patient in the past <NUM> to <NUM> weeks, and is not affected by occasional elevation or reduction of the blood glucose.

A variety of designs are also available in the prior art for testing the concentrations of analytes, for example:
As shown in <FIG>, <CIT> discloses a reaction vessel. The reaction vessel includes a reaction channel and a liquid reagent storage portion, wherein a drying reagent is deployed on the reaction channel; the liquid reagent storage portion is used for storing a buffer solution or other liquid reagent; the liquid reagent storage portion includes a storage body <NUM>' which is sealed by a sealing element or a thin film <NUM>'; and the thin film <NUM>' has a distal end <NUM>', from which the thin film can be directly torn to separate the thin film from the storage body so that the reagent in the storage body is released into the reaction channel.

To release the liquid reagent, it needs to separate the thin film <NUM>' manually which is originally sealed on the storage body <NUM>', and then remove the thin film. Although the thin film can be torn off in the above manner to release the liquid reagent, it is very difficult to tear off the thin film from the storage body <NUM>' through a segment of extended distal end <NUM>', and the thin film is likely to be broken or incompletely torn off in the case of manual tearing with an excessive or insufficient force, such that the liquid reagent cannot be completely released, and the liquid reagent is insufficient during the test, resulting in a deviation of a test result; and on the other hand, in a non-test period, the thin film <NUM>' is exposed to the air, thereby being prone to the risk of tear-off or damage by human or other factors.

As shown in <FIG>, <CIT> discloses a "reagent container and delivery method thereof". The reagent container includes a reagent storage cavity <NUM>' and a sealing element <NUM>' sealed on the reagent storage cavity, wherein the sealing element is configured to be a folded arrangement of two layers, one layer being used for sealing the reagent storage cavity, the other layer extending to the outside of the reagent storage cavity to form an extension segment <NUM>', and the extension segment <NUM>' being used for tearing off the film and releasing the reagent in the reagent storage cavity. To release the liquid reagent, it needs to manually separate the sealing element <NUM>', which is originally sealed on the reagent storage cavity <NUM>', and the thin film is likely to be broken or incompletely torn off in the case of an excessive or insufficient force, such that the liquid reagent cannot be completely released, and the liquid reagent is insufficient during the test, resulting in a deviation of a test result.

As shown in <FIG>, <CIT> discloses a "reaction vessel for testing glycated hemoglobin concentration". The reaction vessel includes a first area used for containing a blood sample of a kit, a second area used for containing a washing solution, a test area and a reagent bag, wherein a reagent and the washing solution in the reagent bag are separately stored and are sealed by an aluminum foil <NUM>'. When the reagent bag is inserted into the reaction vessel, the aluminum foil is torn off by the reaction vessel, the reagent and the washing solution in the reagent bag are temporarily stored in a first reaction area and a second reaction area of the reaction vessel respectively, and sequentially react with the blood sample through rotation of the reaction vessel, thereby solving the storage and distribution problems of the reagent.

During testing with the reaction vessel, firstly, a release portion <NUM>' on the aluminum foil needs to be manually folded, so that the release portion <NUM>' is aligned with a holder in a test cassette, and the aligned reagent bag is inserted into the test cassette, so that the test cassette can cut off the release portion to separate the aluminum foil from the reagent bag. This design is relatively complex, the operation steps are troublesome, and moreover, manual alignment is required for the insertion, and failure of insertion into place is liable to occur which entails repeated insertion. On the other hand, the reaction vessel and the reagent bag are of a separate design, and due to the open design of the reaction vessel, in the case of improper operation, a foreign matter is very likely to drop into the reaction vessel, which affects the accuracy of the test result. Furthermore, in the non-test period, the release portion <NUM>' is exposed to the air, thereby being prone to the risk of tear-off or damage by human or other factor.

The reaction vessel in the prior art includes a sampling needle, a reagent storage device, a reaction portion, a test area and the like. In a test reaction of the reaction vessel, the reagent in the reagent storage device is released to the reaction portion to participate in the test reaction. During the process of releasing the reagent to the reaction portion, as the reaction vessel is relatively small and structurally compact, and the distance between the reagent storage device and the wallboard of the reaction vessel is relatively small, an adsorption force for the reagent liquid is likely to be generated between the wallboard and a reagent release opening of the reagent storage device, such that a part of the reagent is left in a gap between the reagent release opening and the wallboard. In addition, in order to enable the reagent to enter the reaction portion more smoothly, a flow directing tip is further designed at the position of the reagent release opening of the reagent storage device, so that the reagent is guided by the flow directing tip after being released from the reagent release opening and finally enters the reaction portion under the action of gravity. Although the flow directing tip can better achieve flow guide, the flow directing tip is prone to a liquid suspension phenomenon when the reagent is released, so that a part of the reaction reagent is left on the flow directing tip. Furthermore, as the distance between the flow directing tip and the wallboard is relatively small, there is also a part of the reagent absorbed between the flow directing tip and the wallboard. Due to the partial residue of the reaction reagent, the reaction is not sufficient enough, resulting in reduced accuracy of the test result.

<CIT> discloses a biochemical assay cartridge which includes an insertion-type solution cartridge for supplying the sample and a reaction cartridge into which the insertion-type solution cartridge is inserted and received.

<CIT> describes a reagent vessel which is inserted into a measuring cassette for measuring a biological sample.

The technical problem to be solved by the present invention is to provide a testing system in view of the above problems in the prior art.

In order to solve the above problem, according to the present invention, there is provided a testing system according to claim <NUM>.

Preferably, the ejection rod is movable relative to the test cassette.

Preferably, the ejection rod is arranged on a bottom plate of the test cassette.

Preferably, the ejection rod is arranged on an inner side panel of the test cassette.

Preferably, an opening is formed in the reagent reaction vessel, and the ejection rod is configured to penetrate through the opening to cooperate with the push rod.

Preferably, a movable plate is arranged on an inner side face of the test cassette.

Preferably, the movable plate includes a substrate and an elastic element, and the substrate is connected with the test cassette through the elastic element.

Preferably, the substrate is a heating plate.

Preferably, an elastic sheet is arranged on one inner side face of the test cassette.

Preferably, a groove is formed in an inner side face of the test cassette opposite to the movable plate.

The reagent reaction vessel mentioned herein can also be called a reaction vessel, biological sample reaction vessel, biological sample analysis vessel or test reaction vessel.

Compared with the prior art, the present invention has the following beneficial effects:.

The present invention will be further described in detail below in conjunction with the drawings and embodiments, but the protection scope of the present invention is not limited thereto.

As shown in <FIG>, a testing system of the present invention is used for quickly testing the concentration of an analyte in a biological sample, and includes a reagent reaction vessel <NUM> and an external device, the external device is a device other than a body of the reagent reaction vessel <NUM>, wherein the external device is also called a test device, a test cassette <NUM> is arranged on the external device or the test device, and the reagent reaction vessel <NUM> and the test cassette <NUM> are cooperatively used. The reagent reaction vessel <NUM> serves as a reaction container and can achieve storage of a plurality of reagents, sequential addition of a plurality of reagents, waste liquid recycle and other functions. The test cassette <NUM> is used for placing the reagent reaction vessel <NUM>, the test cassette <NUM> is installed on the test device, the test device provides mechanical power and control for rotation of the reagent reaction vessel <NUM>, uniform mixing of the reagents in the reagent reaction vessel <NUM>, reagent release and the like, moreover, an optical device is further arranged on the external device, and the optical device is used for testing the concentration of the analyte in a liquid sample.

As shown in <FIG> and <FIG>, a reagent storage portion <NUM>, a reagent release portion <NUM>, a reaction portion and a test area <NUM> are arranged in the reagent reaction vessel <NUM>. The reagent storage portion <NUM> and the reagent release portion <NUM> are both packaged in the reagent reaction vessel <NUM>. Preferably, a cavity is formed in the reagent reaction vessel <NUM>, and the reagent storage portion <NUM> is contained in the cavity. The reagent storage portion <NUM> is an independent component and is packaged in the reagent reaction vessel <NUM>, which is beneficial for fast assembly of the reagent reaction vessel. The reagent storage portion <NUM> is used for sealing and storing a solid particle, powder or liquid reagent, and the reagent release portion <NUM> can, through cooperation with the test cassette <NUM> in the test device, quickly open the reagent storage portion <NUM> to release the reagent into the reaction portion of the reagent reaction vessel <NUM>. The reaction portion is used for temporary storage, mixing and reaction of the biological sample and the reagent, and an intermediate product or a final product of the reaction is tested through the test area <NUM>.

As shown in <FIG>, the reagent storage portion <NUM> includes at least one reagent containing cavity, and the reagent storage portion <NUM> is sealed by a sealing element <NUM>, namely, the reagent containing cavity is sealed by the sealing element <NUM>. The reagent storage portion <NUM> is also called a reagent storage device, the reagent storage portion <NUM> includes a plurality of reagent containing cavities, and the plurality of reagent containing cavities are arranged in an array. The number of the reagent containing cavities is set according to the categories of the reagents needed in the test reaction and the addition sequence, for example, one, two or more, the reagent containing cavities are independent from each other, namely the plurality of reagent containing cavities are provided with gaps therebetween and are distributed in the array. For example, two reagent containing cavities can be horizontally arranged in a row, can also be longitudinally arranged in a column and can also be diagonally; and as another example, the plurality of reagent containing cavities can also be distributed at intervals in a staggered manner. The capacity of the reagent containing cavities is also set according to the dosage needed in the test reaction.

Preferably, the reagent storage portion includes at least two columns of reagent containing cavities, and each column includes at least one reagent containing cavity. Preferably, as shown in <FIG>, the reagent storage portion <NUM> includes two reagent containing cavities, and the two reagent containing cavities are horizontally arranged on left and right sides. That is, a reagent containing cavity <NUM> and a reagent containing cavity <NUM> are distributed in one row and two columns on the left and right sides, and the bottoms of the reagent containing cavities are approximately located on the same horizontal line. Therefore, when the sealing element <NUM> is torn off, the reagents in the reagent containing cavity <NUM> and the reagent containing cavity <NUM> can be released into the reagent reaction vessel <NUM> together.

As shown in <FIG>, in an embodiment of the reagent storage portion <NUM>, the two reagent containing cavities are longitudinally arranged, namely, a reagent containing cavity <NUM> and a reagent containing cavity <NUM> are distributed in two rows and one column up and down, when the sealing element <NUM> is partially torn off, the reagent containing cavity <NUM> located in the lower row preferentially releases the reagent, and the sealing element <NUM> is further torn off according to the requirement of the reaction time, so that the reagent containing cavity <NUM> located in the upper row releases the reagent. The reagents can be added in sequence by controlling the tear-off degree of the sealing element <NUM> so as to control the implementation of the reaction.

As shown in <FIG>, in another embodiment of the reagent storage portion, four reagent containing cavities are arranged in an array, namely, a reagent containing cavity <NUM>, a reagent containing cavity <NUM>, a reagent containing cavity <NUM> and a reagent containing cavity <NUM> are arranged in two rows and four columns. With the torn-off degree of the sealing element <NUM> being controlled, the reagents in the reagent containing cavity <NUM> and the containing cavity <NUM> in the lower row are preferentially released, and then the reagents in the cavities in the upper row are released.

Preferably, the reagent storage device includes a reagent containing cavity used for storing a solid particle reagent or a powder particle reagent. The solid particle reagent or the powder particle reagent is sealed in the reagent storage device, thereby avoiding random movement of the reagent in the reagent reaction vessel, avoiding the problem that a drying reagent needs to be fixed in the prior art, and meanwhile prolonging the useful life and storage life of the reagent at normal temperature in a sealed state, so that this reagent is distinctive from other reagents, so the reagent storage device is particularly suitable for reagents that are difficult to store under the normal temperature.

Preferably, the solid particle reagent or the powder particle reagent is a freeze-dried solid particle reagent or a freeze-dried powder particle reagent. Preferably, the solid particle reagent is a latex freeze-dried pellet. The latex freeze-dried pellet achieves maximum protection of the reactivity of the latex antibody on the one hand, and greatly prolong the useful life and storage life of the reagent under the normal temperature on the other hand.

Preferably, the reagent storage device further includes a reagent containing cavity used for storing a liquid reagent. The reagent containing cavity can be used for storing a solid particle, powder particle or liquid reagent, and it can be set according to test requirement.

Preferably, as shown in <FIG>, an injection hole <NUM> is formed in at least one reagent containing cavity, the injection hole <NUM> communicates the reagent containing cavity with the external space, and the reagent containing cavity and the injection hole <NUM> are both sealed by the sealing element <NUM>. Preferably, the injection hole <NUM> is formed in the reagent containing cavity used for storing a powder reagent or a liquid reagent. In order to seal the liquid reagent or the powder reagent more simply, in a sealing process, the reagent containing cavity is firstly sealed by the sealing element <NUM> and the injection hole <NUM> is exposed at the outside, then the liquid reagent or the powder reagent is injected to the reagent containing cavity, and thereafter the injection hole <NUM> is sealed so as to guarantee the sealing effect and reduce the difficulty of sealing the liquid reagent or the powder reagent. The reagent containing cavity and the injection hole can be sealed by the same sealing element in different steps and at different time, or can be separately sealed by two sealing elements, for example, the injection hole is sealed by a sealing stopper, and the like.

Preferably, as show in <FIG>, a cavity <NUM> used for mounting a desiccant is further arranged on the back of the reagent storage portion <NUM>. When the reagent storage portion <NUM> is installed in the reagent reaction vessel <NUM>, the desiccant is installed in the cavity <NUM> by means of cooperation between the cavity <NUM> and the inner wall of a panel <NUM>.

Preferably, the reagent storage portion <NUM> includes two reagent containing cavities, and the two reagent containing cavities are respectively used for storing a solid particle reagent and a liquid reagent. The embodiment is illustrated in detail by using an example that the reagent storage portion <NUM> includes two reagent containing cavities <NUM>, <NUM>, the reagent containing cavity <NUM> is used for storing the solid particle reagent, the reagent containing cavity <NUM> is used for storing the liquid reagent, and the injection hole <NUM> is formed in an upper end of the reagent containing cavity <NUM>. In the sealing process, the solid particle reagent is placed in the reagent containing cavity <NUM> at first, cavity openings of the reagent containing cavity <NUM> and the reagent containing cavity <NUM> are sealed by the sealing element <NUM>, then the liquid reagent is injected into the reagent containing cavity <NUM>, and thereafter the injection hole <NUM> is sealed by the sealing element <NUM>.

Preferably, the bottom of the reagent containing cavity is designed into the shape of an inclined plane. When the reagent is released, the reagent can flow out conveniently and quickly, thereby reducing the residual reagent in the containing cavity as much as possible and guaranteeing the accuracy of controlling the volume of the reagent.

As shown in <FIG>, the reagent storage portion <NUM> is sealed by the sealing element <NUM>, namely the reagent is sealed in the reagent containing cavity, the sealing element <NUM> can be torn off from the reagent containing cavity under the action of an external force, the sealing element <NUM> can be torn off from left to right, from bottom to top or the like, description is made herein using an example that the sealing element is torn off from bottom to top, as shown in <FIG>. The sealing element <NUM> can be an aluminum foil, a thin film or other sealing element conventionally used in the prior art.

As shown in <FIG>, <FIG>, the reaction vessel includes the reagent storage portion <NUM> installed in the reaction vessel, a reagent release site is arranged on the reagent storage portion <NUM>, the reaction vessel <NUM> includes a wallboard facing to the reagent release site, a flow guide rib <NUM> is arranged on the wallboard, the flow guide rib <NUM> is in contact with liquid drops on a tail end of the reagent release site, and the flow guide rib <NUM> is used for guiding the flow of the liquid drops on the tail end of the reagent release site. The reagent release site refers to a position where the liquid reagent flows out from the reagent storage device and leaves the reagent storage device, namely, a liquid passage of the liquid reagent on the reagent storage device. Correspondingly, the tail end of the reagent release site refers to the tail end of the liquid passage. The wallboard is a backboard <NUM> or an isolating plate <NUM> on the reaction vessel <NUM> mentioned below, when the flow guide rib <NUM> is installed on the backboard <NUM>, the reagent release portion adopts the manual mode in the prior art, and when the flow guide rib <NUM> is installed on the isolating plate <NUM>, the reagent release portion adopts the automatic mode herein. When the liquid reagent is released from the reagent storage device into the reaction vessel, a part of liquid drops remain on the tail end of the reagent release site or is adsorbed between the tail end of the reagent release site and the wallboard, and the liquid drops are caused to leave the reagent release site along the flow directing direction of the flow guide rib through the contact between the flow guide rib and the liquid drops on the tail end of the reagent release site, thereby avoiding the local residue of the liquid drops of the reagent, ensuring the accuracy of controlling the volume of the reagent and ensuring more sufficient reaction.

In the embodiment shown in <FIG>, the reagent release site includes a reagent release opening <NUM>. The bottom of the reagent release opening <NUM> is the tail end of the reagent release site. In the embodiment shown in <FIG>, the reagent release site further includes a flow guide plate <NUM> connected below the reagent release opening <NUM>. The flow guide plate <NUM> is used for guiding the flow of the liquid, so that the liquid regent leaves the reagent storage portion <NUM> more smoothly, the flow guide plate <NUM> is in the shape of a sharp corner, and the bottom of the flow guide plate <NUM> is the tail end of the reagent release site.

Preferably, the flow guide rib <NUM> is in contact with the tail end of the reagent release site. Therefore, the contact surface of the liquid drops on the tail end of the reagent release site and the flow guide rib <NUM> is increased, and accordingly the flow of the liquid drops is directed more quickly. As shown in <FIG>, the flow guide rib includes a contact end <NUM> and a guide end <NUM>, which are connected successively, the contact end <NUM> is in contact with the liquid drops on the tail end of the reagent release site or in contact with the tail end of the reagent release site, and the guide end <NUM> extends downward from the contact end <NUM>. Preferably, the flow guide rib is in the shape of a thin strip. The contact end <NUM> is used for contacting the liquid drops and directing the flow of the liquid drops onto the guide end <NUM>, the guide end <NUM> is used for guiding the liquid drops on the contact end <NUM> to a specified position of the reaction vessel <NUM>. The area of the cross section of the guide end <NUM> is successively reduced from top to bottom, and the bottom of the guide end is formed into a tip, which is conducive to directing the flow of the liquid drops so as to reduce the liquid suspension phenomenon on the bottom of the guide end as much as possible. Due to the contact of the contact end and the liquid drops, the flow of the liquid drops can be quickly directed. The closer the contact end to the tail end of the reagent release site, the larger the contact surface between the contact end and the liquid drops is, and the more obvious the flow directing function of the flow guide rib is.

As shown in <FIG>, the reaction vessel <NUM> further includes a reaction portion, and the flow guide rib <NUM> projects into the reaction portion. That is, the guide end <NUM> of the flow guide rib <NUM> projects into the reaction portion and comes into contact with the liquid reagent in the reaction portion. By means of the extension design of the flow guide rib <NUM>, the liquid drops can be quickly and accurately guided into the reaction portion. Meanwhile, after the flow of the large liquid drops adsorbed to the tail end of the reagent release site are directed by the flow guide rib, tiny liquid points may be suspended on the bottom of the guide end of the flow guide rib, the liquid drops suspended on the guide end come into contact with the liquid reagent in the reaction portion and then are taken away, thereby avoiding the residual small liquid drops on the flow guide rib. In the embodiment shown in <FIG>, the contact end <NUM> of the flow guide rib <NUM> extends upward, which is conducive to quickly guiding the liquid in the reagent storage device to enter the reaction portion.

In the embodiment shown in <FIG>, the reaction vessel <NUM> includes a side plate, and the flow guide rib <NUM> forms a certain angle with respect to the side plate. The flow guide rib <NUM> is obliquely arranged or arranged to be parallel to the side plate. The side plate is a left side plate <NUM> or a right side plate <NUM> on the reaction vessel <NUM> mentioned below. Preferably, the flow guide direction of the flow guide rib <NUM> is consistent with the flow direction of the liquid in the reagent storage device. When the flow direction of the liquid reagent released from the reagent storage device is vertical to the horizontal plane, the flow guide rib is arranged to be vertical to the horizontal plane; when the reagent storage device of the reaction vessel releases the reagent, the reaction vessel inclines at an angle relative to the horizontal plane, at this time, the flow direction of the liquid reagent forms an inclination angle with respect to the side plate for release, the flow guide rib is obliquely arranged, and the inclination direction of the flow guide rib is the flow direction of the liquid in the reagent storage device. The flow guide direction of the flow guide rib is consistent with the flow direction of the liquid in the reagent storage device, which is conducive to reducing the resistance in a flow directing process to enable the reagent to flow down quickly. Preferably, the flow guide rib is arched. Preferably, the longitudinal section of the flow guide rib is a triangle. The triangle is a rounded triangle. The triangle is selected from a right triangle, an obtuse triangle or an acute triangle. In the embodiment shown in <FIG>, the longitudinal section of the flow guide rib <NUM> is a right triangle, one right angle side of the right triangle is fixedly arranged on the wallboard, the portion on the flow guide rib <NUM> capable of contacting the liquid drops is the contact end <NUM>, and the portion below the contact end <NUM> is the guide end <NUM>. In the embodiment shown in <FIG>, the longitudinal section of the flow guide rib <NUM> is an obtuse triangle, the maximum hypotenuse of the obtuse triangle is fixedly arranged on the wallboard, the portion on the flow guide rib <NUM> capable of contacting the liquid drops is the contact end <NUM>, the portion below the contact end <NUM> is the guide end <NUM>, and the portion above the contact end <NUM> is an extension portion of the contact end <NUM>.

As shown in <FIG>, the flow guide rib <NUM> includes an installation surface <NUM>, a flow guide surface <NUM> opposite to the installation surface and two side flow guide surfaces <NUM> adjacent to the installation surface, and the installation surface <NUM> is fixedly arranged on the wallboard. The flow guide surface <NUM> faces to the reagent release site, the flow guide surface <NUM> is a smooth curved surface, and the junctions of the two side flow guide surfaces <NUM> and the wallboard are smooth curved surfaces. Due to the design of the smooth curved surfaces, the flow directing of the flow guide rib is smoother, and the resistance is smaller.

As shown in <FIG>, the reagent release portion <NUM> includes a push rod <NUM>, the push rod <NUM> is connected to the sealing element <NUM>, and the push rod <NUM> is used for cooperation with an external device to separate the sealing element from the reagent storage portion. As shown in <FIG>, namely, the sealing element <NUM> is adhered or fixed to the push rod <NUM>, when the external device acts on the push rod <NUM>, the push rod <NUM> cooperates with the inner wall of the reagent reaction vessel <NUM> to generate movement relative to the reagent storage portion <NUM>, the push rod <NUM> drives, while moving, the sealing element <NUM> to move relative to the reagent storage portion <NUM>, namely, an action of tearing off the sealing element <NUM>, so that the reagent in the reagent containing cavity is released into the reagent reaction vessel <NUM>. The movement of the push rod <NUM> relative to the reagent storage portion <NUM> can be implemented in a manner of from left to right, from right to left, from bottom to top, etc..

Preferably, as shown in <FIG>, one end of the sealing element <NUM> seals the reagent storage portion <NUM>, and the other end of the sealing element is adhered to the push rod <NUM> after being folded.

Preferably, a force-bearing portion cooperating with the external device is arranged on the push rod <NUM>. The force-bearing portion is used for bearing the thrust provided by the external device.

Preferably, the push rod <NUM> is entirely contained in the reagent reaction vessel <NUM>, and a thrust part of the external device needs to project into the reagent reaction vessel <NUM> or act on the force-bearing portion of the push rod <NUM> in other manner. The push rod <NUM> is entirely contained in the reagent reaction vessel, a human hand cannot touch the push rod in the reagent reaction vessel, the push rod <NUM> cannot be caused to operate manually with bare hands, and the push rod <NUM> can only be caused to operate by using an external tool. Therefore, the possibility of earlier leakage of the reagent in a non-test period caused by manual tear-off or damage of the sealing element <NUM> due to misoperation or other reason is avoided.

Preferably, as shown in <FIG>, a chute <NUM> is formed in the reagent reaction vessel <NUM>. On the one hand, the chute <NUM> embeds the push rod <NUM> in a slide way, and on the other hand, the chute <NUM> is in slide fit with the push rod <NUM>. In an embodiment, the chute <NUM> is formed the backboard <NUM>, the chute <NUM> can be designed to only consist of the backboard <NUM> and two groove sides, and in this case, two side parts of the push rod <NUM> are partially contained in the chute <NUM>.

Preferably, as shown in <FIG>, the isolating plate <NUM> is arranged on the chute <NUM>, and the isolating plate <NUM> isolates the chute <NUM> from the inner space of the reagent reaction vessel <NUM>. In an embodiment, the chute <NUM> is a columnar body with at least one open end, four side faces of the columnar body are respectively formed by part of the backboard <NUM>, two side edges of the chute <NUM> and the isolating plate <NUM>, that is to say, the chute <NUM> is hermetically connected with the isolating plate <NUM>, the isolating plate <NUM> isolates the chute <NUM> from the inner space of the reagent reaction vessel <NUM>, and the height of the isolating plate <NUM> is designed in such a way that the reaction liquid will not flow to the outside of the reagent reaction vessel. The chute <NUM> is designed into an independent area, the push rod <NUM> or an external thrust part is isolated from the reagent, therefore it can be avoided that a part of reagent is taken away by the contact of the push rod <NUM> or the external thrust part and the reaction reagent.

Preferably, as shown in <FIG>, at least one limiting projection <NUM> is arranged on at least one side face of the push rod <NUM>. The limiting projection <NUM> allows the push rod <NUM> to be installed in the reagent reaction vessel <NUM> more stably. Preferably, there are two limiting projections <NUM>, which are respectively arranged on left and right side faces of the push rod <NUM>. Preferably, the limiting projections <NUM> are arranged on the upper parts of the side faces of the push rod <NUM>. When the push rod <NUM> is installed in the reagent reaction vessel <NUM>, the limiting projections <NUM> on the push rod <NUM>, squeezed by the reagent reaction vessel <NUM>, causes the push rod <NUM> to generate elastic deformation, therefore the friction force between the push rod <NUM> and the reagent reaction vessel <NUM> is increased, so that the push rod <NUM> can be installed in the reagent reaction vessel <NUM> stably, and the resistance during movement of the push rod <NUM> is also increased.

Preferably, as shown in <FIG>, at least one hollowed-out slot <NUM> is arranged at a position of the push rod <NUM> close to an edge, the limiting projection <NUM> is arranged on the outer side wall of the slot <NUM>, and the limiting projection <NUM> and the slot <NUM> are arranged in pairs. In a specific embodiment, there are two limiting projections <NUM>, the two limiting projections <NUM> are respectively arranged on the left and right side faces of the push rod <NUM>, correspondingly, there are also two hollowed-out slots <NUM>, which are respectively arranged at positions on the left side and the right side of the push rod close to edges, namely the outer side of the left side slot <NUM> is the left side face of the push rod <NUM>, the outer side wall of the right side slot <NUM> is the right side face of the push rod <NUM>, that is to say, the limiting projections <NUM> are arranged on the outer side walls of the slots <NUM>. Due to the arrangement of the hollowed-out slots <NUM>, the push rod <NUM> is more liable to generate deformation during action, thereby avoiding inflexible slide of the push rod <NUM> due to overlarge friction force.

If the push rod <NUM> is in a deformation state for a long time, the push rod is liable to lose certain elasticity, and thus the fastening effect is poor. In order to solve the above problem, preferably, as shown in <FIG>, a limiting groove <NUM> cooperating with the limiting projection <NUM> is formed in the reagent reaction vessel <NUM>. Preferably, the limiting groove <NUM> for clamping the limiting projection <NUM> is formed in the chute <NUM>, and the number of the limiting groove <NUM> is the same as that of the limiting projection <NUM>. When the push rod <NUM> is in an initial state, namely, a normal state, the limiting groove <NUM> clamps the limiting projection <NUM>, and the push rod <NUM> generates no deformation at this time; and when the push rod <NUM> is caused to slide by an external force, the push rod <NUM> generates deformation at the limiting projection <NUM> and leaves the limiting groove <NUM>.

Preferably, as shown in <FIG>, a groove <NUM> is formed in the push rod. The groove <NUM> is formed in the upper end of the push rod <NUM>, the groove <NUM> is an adhesion groove for adhering the sealing element <NUM>. Before the sealing element is adhered, the plane where the bottom of the groove <NUM> is located is slightly lower than the plane where the notch of the groove <NUM> is located, and after the sealing element <NUM> is adhered, the sealing element <NUM> fills the groove <NUM>, so that the push rod has a flat surface. The probability that the sealing element <NUM> is stripped off due to being higher than the surface of the push rod <NUM> when the push rod <NUM> is pushed is avoided.

Preferably, the force-bearing portion of the push rod <NUM> is the bottom surface or the back of the push rod <NUM>, when the force-bearing portion is the bottom of the push rod, the external force acts on the bottom surface of the push rod, so that the push rod <NUM> tears off the sealing element <NUM> from bottom to top, and when the force-bearing portion is the back of the push rod, the external force acts on the back of the push rod and also causes the push rod <NUM> to tear off the sealing element <NUM> from bottom to top. The force-bearing portion of the push rod <NUM> can also be an upper bottom surface, the left side face or the right side face of the push rod, when the force-bearing portion of the push rod is the upper bottom surface, the push rod can be pulled from above; when the force-bearing portion of the push rod is the left side face, the push rod can be pushed from left to right; and when the force-bearing portion of the push rod is the right side face, the push rod can be pushed from right to left.

In another embodiment, the force-bearing portion of the push rod <NUM> is in magnetic connection with an external thrust part, namely, the force-bearing portion of the push rod <NUM> and the external thrust part are magnetic components that attract each other, such as iron blocks, magnets or the like. The push rod <NUM> and the external thrust part are isolated by the reagent reaction vessel <NUM>, and the external thrust part drives the push rod <NUM> to operate under magnetic action.

Preferably, an opening is formed in the reagent reaction vessel <NUM>, the force-bearing portion is exposed in the opening, and the force-bearing portion receives external thrust through the opening. Namely, the external thrust part can project into the opening, come into contact with the push rod <NUM> in the reagent reaction vessel <NUM> and cause the push rod <NUM> to operate, and the setting of the position of the opening is associated with the setting of the force-bearing portion of the push rod <NUM>.

In an embodiment, as shown in <FIG>, the force-bearing portion of the push rod <NUM> is the bottom surface of the push rod, the foregoing opening <NUM> is formed in the bottom plate <NUM> of the reagent reaction vessel <NUM>. Preferably, as shown in <FIG>, a chamfer <NUM> is arranged on the opening <NUM>, and the push rod <NUM> is completely contained in the chute <NUM> and at a distance from the opening <NUM> so as to avoid collision during misoperation. The external thrust part is introduced from the chamfer <NUM> of the opening <NUM> and projects into the chute <NUM> to contact the push rod <NUM> and bring the push rod <NUM> and the external thrust part into slide fit with the chute <NUM>.

In another embodiment, as shown in <FIG>, the force-bearing portion of the push rod <NUM> is the back of the push rod <NUM>, and the foregoing opening <NUM> is formed in the backboard <NUM> of the reagent reaction vessel <NUM>. Preferably, a convex edge or a recess <NUM> is arranged on the back of the push rod <NUM>, the convex edge or the recess <NUM> is the force-bearing portion, and the convex edge or the recess <NUM> is exposed in the opening <NUM>. The external thrust part comes into contact with the convex edge or the recess <NUM> and brings the push rod <NUM> into slide fit with the chute <NUM>.

The reaction portion includes at least one reaction area, and the reaction area receives a reagent released by the reagent storage portion <NUM>. The reaction portion includes a plurality of reaction areas, the setting of the number and positions of the reaction areas is related to the number of the reagent containing cavities in the reagent storage portion <NUM> and the reaction steps, for example, two reagents released at the same time can be temporarily stored in one reaction area and can also be temporarily stored in two independent reaction areas respectively; if a drying reagent is further deployed in the reaction area, other reaction area can also be arranged separately, the reaction areas are communicated with each other, and the reagents in the reaction areas can be mixed by rotating the reagent reaction vessel.

As shown in <FIG> and <FIG>, a test area <NUM> is arranged in the reaction portion, the test area <NUM> can be arranged on a flow passage of any reagent of the reagent reaction vessel <NUM>, and the test area <NUM> is generally made of a transparent material, so that transmission light or scattered light emitted by an optical device can enter the reagent reaction vessel <NUM>. A flow guide element is arranged between the reagent storage portion <NUM> and the reaction portion, more specifically, the flow guide element is arranged between the reagent containing cavity and the corresponding reaction area, and the flow guide element enables the reagent in the reagent containing cavity to be released into the reaction area quickly and accurately.

Preferably, as shown in <FIG> and <FIG>, the reaction portion includes at least one reaction area, wherein at least one reaction area is a first reaction area <NUM>, the first reaction area <NUM> is used for temporarily storing a solid particle reagent, the first reaction area <NUM> includes a supporting portion and a blocking portion, a gap <NUM> is formed between the supporting portion and the blocking portion, and the maximum width of the gap <NUM> is smaller than the minimum width of the solid particle reagent. The gap between the supporting portion and the blocking portion is used for preventing the solid particle reagent in the first reaction area from entering other reaction area(s), so that the solid particle reagent can be temporarily stored on the supporting portion stably. In an embodiment, the supporting portion is a step <NUM>, and the blocking portion is a baffle, marked as a first baffle <NUM>. The first baffle <NUM> is a vertical baffle, and the gap <NUM> is formed between the bottom of the first baffle <NUM> and the supporting portion. Preferably, the solid particle reagent is a latex freeze-dried pellet reagent. The minimum width of the latex freeze-dried pellet reagent, namely the diameter of the latex freeze-dried pellet is larger than the maximum width of the gap <NUM>, so that the latex freeze-dried pellet reagent is blocked by the gap <NUM>.

Preferably, as shown in <FIG> and <FIG>, the first reaction area <NUM> further includes a second baffle <NUM>, the second baffle <NUM> is obliquely arranged, the second baffle <NUM> and the blocking portion from a second gap <NUM>, the minimum width of the second gap <NUM> is larger than the maximum width of the solid particle reagent. The second baffle <NUM> and the blocking portion are used for directing the flow of the solid particle reagent, so that the solid particle reagent can smoothly enter the first reaction area <NUM>. In a specific embodiment, the maximum width of the latex freeze-dried pellet reagent, namely the diameter of the latex freeze-dried pellet is smaller than the minimum width of the second gap <NUM>, so that the latex freeze-dried pellet reagent can conveniently enter the first reaction area <NUM>.

Preferably, the first baffle <NUM> and the second baffle <NUM> are baffles with radians. On the one hand, the solid particle reagent can enter quickly, and difference of diameters of solid particles is considered to avoid that the solid particles are clamped between the first baffle <NUM> and the second baffle <NUM> and cannot drop onto the step <NUM>.

Preferably, the reaction portion further includes a second reaction area, and the second reaction area is used for temporarily storing a liquid reagent. Preferably, a flow guide element is arranged on the second reaction area, and the flow guide element includes a first flow directing plate and a second flow directing plate.

In a specific embodiment, as shown in <FIG> and <FIG>, the reaction portion includes a first reaction area <NUM> and a second reaction area <NUM>, the first reaction area <NUM> is used for receiving the solid particles released by the reagent containing cavity <NUM>, and the second reaction area <NUM> is used for receiving the liquid reagent released by the reagent containing cavity <NUM>. A flow guide element is arranged on the second reaction area <NUM>, and the flow guide element includes a first flow directing plate <NUM> and a second flow directing plate <NUM>, so that the liquid reagent quickly flows into the second reaction area <NUM>. The first reaction area <NUM> includes the step <NUM> for temporarily storing the reagent, the first baffle <NUM> and the second baffle <NUM>, the first baffle <NUM> and the second baffle <NUM> are used for guiding the solid particles into the step <NUM>, furthermore the gap <NUM> is formed between the first baffle <NUM> and the step <NUM>, and the gap <NUM> can prevent the solid particles from entering the second reaction area <NUM> and allow the liquid reagent to flow into the first reaction area <NUM>.

As shown in <FIG>, the reagent reaction vessel <NUM> includes a top plate <NUM>, the bottom plate <NUM>, a left side plate <NUM>, a right side plate <NUM>, a panel <NUM> and the backboard <NUM>. The reagent reaction vessel <NUM> is approximately a square box body and is made of a plastic material.

Preferably, as shown in <FIG>, one end of the left side plate <NUM> or the right side plate <NUM> of the reagent reaction vessel <NUM> is an inclined plane <NUM>. Preferably, an extension portion <NUM> is arranged on the backboard <NUM> of the reagent reaction vessel <NUM>, and the extension portion <NUM> extends to the outside of the inclined plane <NUM>. Preferably, the inclined plane <NUM> is arranged at a lower left corner of the reagent reaction vessel <NUM>. In an embodiment, the backboard <NUM> is square, the panel <NUM> is pentagonal, the left side plate <NUM> comprises a side face and an inclined plane, the extension portion <NUM> is a right triangle, and the hypotenuse of the right triangle is the connection between the backboard <NUM> and the extension portion <NUM>. Due to the arrangement of the inclined plane <NUM> and the extension portion <NUM>, it is convenient to manually distinguish the front and back surfaces of the reagent reaction vessel <NUM>, thereby avoiding inverted insertion of the reagent reaction vessel <NUM> into the test cassette <NUM>, and on the other hand, when the reagent reaction vessel cooperates with the test cassette, the front and back surfaces of the reagent reaction vessel can be automatically identified to avoid misoperation.

Preferably, as shown in <FIG>, the reagent reaction vessel <NUM> comprises a locating projection <NUM>. The locating projection <NUM> is arranged on the panel <NUM>, the locating projection <NUM> is an inverted triangle, as shown in <FIG>, the locating projection <NUM> is used for firmly locating the reagent reaction vessel <NUM> in a corresponding groove of the test cassette <NUM>, and the locating projection <NUM> is clamped by the groove, so that the reagent reaction vessel <NUM> is fastened in the test cassette <NUM>, thereby avoiding the displacement of the reagent reaction vessel <NUM> relative to the test cassette <NUM> during rotation.

Preferably, as shown in <FIG>, a handle <NUM> is arranged on the top plate <NUM> of the reagent reaction vessel <NUM>, during testing, it is convenient for the user to quickly insert the reagent reaction vessel <NUM> into the test cassette <NUM> by holding the handle <NUM>, and after the test is completed, the reagent reaction vessel <NUM> is quickly pulled out. Preferably, a notch <NUM> is formed in the backboard <NUM> of the reagent reaction vessel <NUM>, and due to the arrangement of the notch <NUM>, the injection molding of the reagent reaction vessel <NUM> is more convenient.

In a specific embodiment, as shown in <FIG>, <FIG> and <FIG>, the reagent storage portion <NUM>, the reagent release portion <NUM>, the reaction portion, the test area <NUM>, a sampling bar <NUM> and a liquid absorption pad <NUM> are arranged in the reagent reaction vessel <NUM>, and the reagent reaction vessel <NUM>, the reagent storage portion <NUM>, the reagent release portion <NUM> and the sampling bar <NUM> are made of a plastic material. The sampling bar <NUM> is used for collecting a liquid sample, such as blood, urine or the like, and adding the liquid sample to the reagent reaction vessel <NUM>. After the test is completed, the liquid absorption pad <NUM> is used for recycling waste liquid so as to avoid leakage of the liquid to cause pollution.

As shown in <FIG>, <FIG> and <FIG>, the reagent reaction vessel <NUM> is a hollow cavity and can be divided into a box body <NUM> and an upper cover <NUM>, the box body <NUM> includes the backboard <NUM>, the bottom plate <NUM>, the left side plate <NUM> and the right side plate <NUM>, the upper cover <NUM> includes the top plate <NUM> and the panel <NUM>, and the upper cover <NUM> and the box body <NUM> are hermetically connected by welding or in other manner, thereby facilitating the assembly of the reagent reaction vessel <NUM>. The liquid absorption pad <NUM> is arranged above the inclined plane <NUM>, the sampling bar <NUM> and the reagent storage portion <NUM> are arranged, next to the liquid absorption pad <NUM>, successively in the direction toward the right side plate <NUM>, the reaction portion and the reagent release portion <NUM> are both arranged below the reagent storage portion <NUM>, and the test area <NUM> is arranged in the reaction portion. The sampling bar <NUM> is arranged in the reagent reaction vessel <NUM> through an opening, and the sampling bar <NUM> is in clearance fit with the opening, thereby avoiding the entry of foreign matters during sampling. The inclined plane <NUM> is arranged on the left side of the sampling bar <NUM>, which is beneficial for sufficient contact between the sample on the sampling bar <NUM> and the reaction liquid, and avoids that the sample cannot be contacted due to too little reaction liquid. The layout of the liquid absorption pad <NUM>, the reagent storage portion <NUM>, the sampling bar <NUM>, the reaction portion and the reagent release portion <NUM> is not limited to that described above. Preferably, the second flow directing plate <NUM> is connected with the upper end of the first baffle <NUM>, the upper end of the first flow directing plate <NUM> is further connected with a sampling needle guide plate <NUM>, and the sampling needle guide plate <NUM> is arranged in a vertical direction. As shown in <FIG> and <FIG>, the box body <NUM> and the upper cover <NUM> both comprise sampling needle fixing parts <NUM>, and the sampling needle fixing parts <NUM> are used for avoiding shaking of the box body and the upper cover.

In a specific embodiment, the reagent storage portion and the reagent reaction vessel are connected in such a manner as shown in <FIG>, wherein locating elements are arranged on the reagent storage portion <NUM>, the locating elements constitute a cavity for containing the reagent storage portion <NUM>, so that the reagent storage portion <NUM> is fixedly installed in the reagent reaction vessel <NUM>. The cavity can also adopt conventional technical means in the prior art. In a specific embodiment, as shown in <FIG>, <FIG>, a locating column <NUM> and a supporting plate <NUM> are arranged on the panel <NUM>, left side locating plates <NUM> and right side locating plates <NUM> are a plurality of dispersive locating elements, which are dispersively arranged on the box body <NUM> and the upper cover <NUM>, and the above locating elements are used for fixedly connecting the reagent storage portion <NUM>. As shown in <FIG> and <FIG>, the locating elements of the reagent storage portion <NUM> include a mounting hole <NUM>, a left locating element <NUM>, a right locating element <NUM> and a lower locating element <NUM>. As shown in <FIG>, the mounting hole <NUM> is sleeved on the locating column <NUM> for fixing the upper end of the reagent storage portion <NUM>; and the left locating element <NUM> and the right locating element <NUM> are respectively in limiting connection with the left side locating plates <NUM> and the right side locating plates <NUM>, and the lower locating element <NUM> is placed on the supporting plate <NUM>. Therefore, the reagent storage portion <NUM> is stably installed in the reagent reaction vessel <NUM>, and the displacement of the reagent storage portion <NUM> during movement or shaking of the reagent reaction vessel <NUM> is avoided.

The preferred embodiments and implementations mentioned above can be randomly selected and combined according to requirement to achieve the ultimate objective of fast sample concentration testing.

As shown in <FIG>, an ejection rod <NUM> is arranged in the test cassette <NUM>, and the ejection rod <NUM> comes into contact with the force-bearing portion through the opening <NUM> in the reagent reaction vessel and provides an acting force of the external device.

Preferably, the ejection rod <NUM> is movable relative to the test cassette <NUM>. The ejection rod <NUM> may be fixedly installed on the box body, and may also be movable relative to the box body. If the ejection rod <NUM> is movable, the external device controls a movement area and a movement position of the ejection rod <NUM>, for example, the movement of the ejection rod <NUM> can be controlled by a motor, and conventional technology in the prior art can also be adopted. If the ejection rod <NUM> is fixed to the box body, when the reagent reaction vessel <NUM> is inserted into the test cassette <NUM> of the external device, the ejection rod <NUM> and the push rod <NUM> are brought into cooperation by means of an insertion force so as to drive the push rod <NUM> to operate.

Preferably, the ejection rod <NUM> is arranged on the bottom plate of the test cassette <NUM> or the ejection rod <NUM> is arranged on inner side panel of the test cassette <NUM>. As shown in <FIG>, when the test cassette <NUM> cooperates with the force-bearing portion which is the bottom surface of the push rod <NUM>, the opening <NUM> is formed in the bottom plate <NUM> of the reagent reaction vessel <NUM>, and the ejection rod <NUM> is arranged on the bottom plate of the test cassette <NUM>. Preferably, a chamfer is arranged on the upper end part of the ejection rod <NUM>, so that the ejection rod <NUM> can conveniently project into the opening <NUM>. When the test cassette <NUM> cooperates with the force-bearing portion which is the back of the push rod <NUM>, the opening <NUM> is formed in the backboard <NUM> of the reagent reaction vessel <NUM>, and the ejection rod <NUM> is arranged on the inner side panel of the test cassette <NUM>.

Preferably, as shown in <FIG>, a movable plate is arranged in the test cassette <NUM>, the movable plate includes a substrate <NUM> and an elastic element <NUM>, and the substrate <NUM> is fixedly connected to the inner wall of the test cassette <NUM> through the elastic element <NUM>. Preferably, two elastic elements <NUM> are provided, each elastic element <NUM> includes an installation surface <NUM> and two elastic arms <NUM>, the installation surface <NUM> is fixedly connected with the inner side face of the test cassette <NUM>, and the two elastic arms <NUM> are fixedly connected with the substrate <NUM> respectively.

Preferably, the substrate <NUM> is a heating plate, which is marked as a first heating plate. That is, the movable plate serves as both a heating element and an elastic fastener.

Preferably, as shown in <FIG>, an elastic sheet <NUM> is arranged on one inner side face of the test cassette <NUM>. Preferably, the elastic sheet <NUM> is arranged on one inner side face adjacent to the movable plate. The elastic sheet <NUM> comprises an elastic arm <NUM>, one end of the elastic arm <NUM> is fixed to the elastic sheet <NUM>, the other end of the elastic arm <NUM> is in the shape of a smooth curved surface, and the elastic arm <NUM> is installed facing to the inner hollow cavity of the test cassette <NUM>. The elastic sheet <NUM> is used for cooperating with the inclined plane <NUM> on the reagent reaction vessel <NUM>, therefore one side of the reagent reaction vessel <NUM> provided with no inclined plane is tightly fit to the inner side wall of the test cassette <NUM>. As the elastic sheet <NUM> has certain elasticity, in the case of inverted insertion of the reagent reaction vessel <NUM> into the test cassette <NUM>, the reagent reaction vessel <NUM> cannot be completely fit into the test cassette <NUM> due to the effect of the elastic sheet <NUM>, therefore correct insertion of the reagent reaction vessel <NUM> can be identified by means of the cooperation of the elastic sheet <NUM> and the inclined plane <NUM>.

Preferably, as shown in <FIG>, a groove <NUM> is formed in one inner side face of the test cassette <NUM>. Preferably, the groove <NUM> is formed in the inner side face opposite to the movable plate. The groove <NUM> is used for clamping the locating projection <NUM> on the reagent reaction vessel <NUM>, so that the reagent reaction vessel <NUM> can be clamped on the groove <NUM>, thereby avoiding the displacement of the reagent reaction vessel <NUM> during rotation or shaking of the test cassette <NUM>.

In a specific embodiment, as shown in <FIG>, <FIG>, the test cassette <NUM> is fixed to the external device to achieve uniform mixing or rotation, the test cassette <NUM> includes a box body with an open end, an optical aperture <NUM> is formed in the box body, the ejection rod <NUM>, a first heating plate <NUM> and a second heating plate <NUM> are arranged in the box body, the first heating plate <NUM> is the movable plate, the ejection rod <NUM> comes into contact with the force-bearing portion through the opening <NUM> and provides external thrust, and the first heating plate <NUM> and the second heating plate <NUM> are used for heating the reagent so as to satisfy the requirement of the reaction temperature.

As shown in <FIG>, when the reagent reaction vessel <NUM> is inserted into the test cassette <NUM>, the reagent reaction vessel <NUM> presses the first heating plate <NUM>, the first heating plate <NUM> causes the elastic element <NUM> to generate deformation under the action of pressure, and then the reagent reaction vessel <NUM> can be quickly inserted, and the locating projection <NUM> is buckled into the groove <NUM>. Meanwhile, the inclined plane <NUM> of the reagent reaction vessel <NUM> compresses the elastic sheet <NUM>, so that the test area <NUM> on the reagent reaction vessel <NUM> is aligned with the optical aperture <NUM> in the test cassette <NUM>. During insertion of the reagent reaction vessel <NUM> into the test cassette <NUM>, the ejection rod <NUM> projects into the opening <NUM> to contact the push rod <NUM>, when the reagent reaction vessel <NUM> moves from top to bottom, the push rod <NUM> moves from bottom to top to drive the sealing element <NUM> to move from bottom to top, so that the sealing element <NUM> is torn off from the reagent storage portion <NUM> to release the reagent, as shown in <FIG>.

At present, there are many methods for testing glycosylated hemoglobin available in the market, wherein the commonly used test methods include ion exchange chromatography, affinity chromatography, high pressure liquid phase, immunization, ion capture and electrophoresis methods and the like. The immunization method means that after erythrocytes are dissolved, HbAlc is measured based on the interaction of antigen molecules and special antibodies.

The test of HbAlc by the immunoagglutination method includes the following two test steps: respectively testing the concentration of total hemoglobin Hb and the concentration of glycated hemoglobin HbAlc in a sample. The test of the total hemoglobin (Hb) includes: oxidizing ferrous ions in the hemoglobin by using potassium ferricyanide to generate methemoglobin, carrying out the reaction of the methemoglobin with thiocyanate to generate thiocyanic acid methemoglobin, and testing the light absorption value at <NUM> to obtain the concentration of Hb. The test of the glycated hemoglobin (HbA1c) includes: a lectin containing a plurality of HbAlc immunoreaction binding sites competes with HbAlc in the blood to combine with an anti-HbAlc antibody marked on a latex microsphere, wherein the combination of the former will lead to a change of the turbidity of the reaction liquid, and the concentration of the HbAlc in the blood can be obtained by testing the light absorption value at <NUM>. The higher the concentration of the HbAlc in the blood, the lower the turbidity is, the smaller the light absorption value is, and the variations of the light absorption value and the concentration of the HbAlc are obtained by a calibration curve.

Therefore, in the test of the HbAlc by using the immunization method, a thiocyanate liquid reagent (Buffer), a latex pellet marked with the anti-HbAlc antibody, a potassium ferricyanide drying reagent (drying object) and a lectin drying reagent (drying object) containing a plurality of HbAlc immunoreaction binding sites need to be used. As shown in <FIG>, the thiocyanate liquid reagent <NUM> is stored in the reagent containing cavity <NUM>, the latex pellet <NUM> marked with the anti-HbAlc antibody is stored in the reagent containing cavity <NUM>, the potassium ferricyanide drying reagent <NUM> is cured on the second reaction area <NUM>, and the lectin drying reagent <NUM> is cured in the first reaction area <NUM>.

The latex pellet is a small latex freeze-dried pellet, a specific HbAlc antibody is connected to the small latex pellet through a covalent binding method in advance, and the latex pellet is quickly frozen to the small pellet having the same volume by using the freeze drying technology, thereby maximally protecting the reactivity of the latex antibody and greatly prolonging the useful life and storage life at normal temperature. The concentration of the HbAlc in the blood is tested by using the reagent reaction vessel of the present invention. The steps of testing the HbAlc through the immunization method are as follows:.

After the test of the HbAlc is ended, the external device calculates and outputs a test structure.

The reagent reaction vessel of the present invention is not limited to the test of the HbAlc in the above blood sample, can also be applied to the test of other biological samples, such as urine, saliva, spinal fluid and the like, and can also be applied to the test of the concentration of C-reactive protein, cholesterol, blood fat, blood glucose and other analytes.

Claim 1:
A testing system, comprising a reagent reaction vessel (<NUM>) and a test device, wherein
a reagent storage portion (<NUM>) and a push rod (<NUM>) movable relative to the reagent storage portion (<NUM>) are packaged in the reagent reaction vessel (<NUM>), the reagent storage portion (<NUM>) comprises at least one reagent containing cavity (<NUM>, <NUM>), and the reagent containing cavity (<NUM>, <NUM>) is being sealed by a sealing element (<NUM>);
the push rod (<NUM>) is connected to the sealing element (<NUM>), and the push rod (<NUM>) is configured to be used for cooperation with the test device to separate the sealing element (<NUM>) from the reagent storage portion (<NUM>); and
the test device comprises a test cassette (<NUM>), an ejection rod (<NUM>) is arranged in the test cassette (<NUM>), and the ejection rod (<NUM>) is configured to cooperate with the push rod (<NUM>) to separate the sealing element (<NUM>) from the reagent storage portion (<NUM>), the push rod (<NUM>) being entirely contained in the reagent reaction vessel (<NUM>).