Patent Description:
The present invention relates to a reliable, specific and sensitive serological diagnosis test of syndrome (SARS)-associated coronavirus infections, in particular a novel strain of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS CoV-<NUM>) infection and a SARS-CoV infection.

Coronavirus is a virus containing single-stranded RNA, of positive polarity, of approximately <NUM> kilobases which replicates in the cytoplasm of the host cells; the <NUM>' end of the genome has a capped structure and the <NUM>' end contains a polyA tail. This virus is enveloped and comprises, at its surface, structures called spicules.

The genome comprises the following open reading frames or ORFs, from its <NUM>' end to its <NUM>' end: ORF1a and ORF1b corresponding to the proteins of the transcription-replication complex, and ORF-S, ORF-E, ORF-M and ORF-N corresponding to the structural proteins S, E, M and N. It also comprises ORFs corresponding to proteins of unknown function encoded by: the region situated between ORF-S and ORF-E and overlapping the latter, the region situated between ORF-M and ORF-N, and the region included in ORF-N.

The S protein is a membrane glycoprotein (<NUM>-<NUM> kDa) which exists in the form of spicules or spikes emerging from the surface of the viral envelope. It is responsible for the attachment of the virus to the receptors of the host cell and for inducing the fusion of the viral envelope with the cell membrane.

The small envelope protein (E), also called sM (small membrane), which is a nonglycosylated transmembrane protein of about <NUM> kDa, is the protein present in the smallest quantity in the virion. It plays a powerful role in the coronavirus budding process which occurs at the level of the intermediate compartment in the endoplasmic reticulum and the Golgi apparatus.

The M protein or matrix protein (<NUM>-<NUM> kDa) is a more abundant membrane glycoprotein which is integrated into the viral particle by an M/E interaction, whereas the incorporation of S into the particles is directed by an S/M interaction. It appears to be important for the viral maturation of coronaviruses and for the determination of the site where the viral particles are assembled.

The N protein or nucleocapsid protein (<NUM>-<NUM> kDa) which is the most conserved among the coronavirus structural proteins is necessary for encapsidating the genomic RNA and then for directing its incorporation into the virion. This protein is probably also involved in the replication of the RNA.

When the host cell is infected, the reading frame (ORF) situated in <NUM>' of the viral genome is translated into a polyprotein which is cleaved by the viral proteases and then releases several nonstructural proteins such as the RNA-dependent RNA polymerase (Rep) and the ATPase helicase (Hel). These two proteins are involved in the replication of the viral genome and in the generation of transcripts which are used in the synthesis of the viral proteins. The mechanisms by which these subgenomic mRNAs are produced are not completely understood; however, recent facts indicate that the sequences for regulation of transcription at the <NUM>' end of each gene represent signals which regulate the discontinuous transcription of the subgenomic mRNAs.

The proteins of the viral membrane (S, E and M proteins) are inserted into the intermediate compartment, whereas the replicated RNA (+ strand) is assembled with the N (nucleocapsid) protein. This protein-RNA complex then combines with the M protein contained in the membranes of the endoplasmic reticulum and the viral particles form when the nucleocapsid complex buds into the endoplasmic reticulum. The virus then migrates across the Golgi complex and eventually leaves the cell, for example by exocytosis. The site of attachment of the virus to the host cell is at the level of the S protein.

Coronaviruses are responsible for <NUM> to <NUM>% of colds in humans and for respiratory and digestive infections in animals, especially cats (FIPV: Feline infectious peritonitis virus), poultry (IBV: Avian infectious bronchitis virus), mice (MHV: Mouse hepatitis virus), pigs (TGEV: Transmissible gastroenterititis virus, PEDV: Porcine Epidemic diarrhea virus, PRCoV: Porcine Respiratory Coronavirus, HEV: Hemagglutinating encephalomyelitis Virus) and bovines (BCoV: Bovine coronavirus).

In <NUM>, a new coronavirus named SARS CoV-<NUM> that causes COVID-<NUM>, was isolated, in association with cases of severe acute respiratory syndrome. The complete genome sequence of SARS CoV-<NUM> is available at GenBank accession no.

The sequence of SARS CoV-<NUM> has been compared to other coronaviruses. Overall, the genome of SARS CoV-<NUM> has <NUM>% nucleotide identity with bat SARS-like-CoVZXC21 and <NUM>% with that of human SARS-CoV (also referred as SARS-CoV-<NUM> in the present application). The organization of the genome is comparable with human SARS-CoV.

New reagents for the detection and diagnosis of SARS CoV-<NUM> and SARS-CoV, which are sufficiently sensitive and specific are needed. The present invention fulfills this need.

The invention as set out in the appended claims is directed to methods and kits for the detection and diagnosis of a SARS-associated coronavirus, such as a SARS CoV-<NUM> infection and SARS-CoV infection, using the N protein of SARS-CoV-<NUM> expressed and purified from E. coli strain BL21 (DE3) pDIA17 transformed with recombinant plasmid pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter or pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter deposited at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, <NUM>, Rue du Docteur Roux, <NUM> Paris, FR, on May <NUM>, <NUM>, under the deposit numbers CNCM I-<NUM> and CNCM I-<NUM>, respectively, also referred as N_SARS2 protein, SARS-CoV-<NUM> N and N_SARS2 in the present application; in some embodiments, N_SARS2 protein is used with the N protein of SARS-CoV, also referred as N_SARS1 protein, SARS-CoV-<NUM> N and N_SARS1 in the present application.

The invention is directed to a method for the detection of a SARS-associated coronavirus infection in a biological sample comprising providing a N_SARS2 protein according to the present disclosure; providing a biological sample from an individual or patient suspected to be infected with a SARS-CoV coronavirus; contacting said N_SARS2 protein with said biological sample; and visualizing the antigen-antibody complexes formed. Preferably, the method comprises an ELISA. Preferably, the N_SARS2 protein comprises or consists of the sequence of SEQ ID NO:<NUM>.

The invention is directed to a method for the detection of a SARS-associated coronavirus infection in a biological sample comprising providing a N_SARS1 protein and a N_SARS2 protein according to the present disclosure; providing a biological sample from an individual or a patient suspected to be infected with a SARS CoV-<NUM> coronavirus; contacting said N_SARS1 and N_SARS2 proteins with said biological sample; and visualizing the antigen-antibody complexes formed. Preferably, the method comprises an ELISA.

The invention is directed to a kit for the detection of a SARS-CoV coronavirus infection in a biological sample comprising a N_SARS2 protein according to the present disclosure. Preferably, the kit comprises serum from an animal immunized with a N_SARS2 protein. Preferably, the kit comprises a N_SARS1 protein.

The invention is directed to compositions, kits, uses, and methods for the detection and diagnosis of SARS-CoV-<NUM> and SARS infections as set out in the appended set of claims.

The indirect ELISA test was used with serum from COVID-<NUM> patients with confirmed infections, as well as from various symptomatic patients (untested for SARS CoV-<NUM> RNA) and presumptively negative patients.

The test was able to detect anti- SARS CoV-<NUM> antibodies in the serum of all of COVID-<NUM> patients with confirmed infections, as well as in the serum of many of the symptomatic patients (untested for SARS CoV-<NUM> RNA). Anti- SARS CoV-<NUM> antibodies were not detected in the serum of presumptively negative patients. These experiments showed that the N protein of SARS-CoV ("N_SARS1") was able to bind to antibodies present in the serum of SARS CoV-<NUM> infected individuals. Thus, the antibodies generated against the N protein of SARS CoV-<NUM> ("N_SARS2") by the patients could bind to the N_SARS1 protein. This allows the use of the N_SARS1, for example using an indirect ELISA, for the detection of SARS CoV-<NUM> via immunoassay.

The N_SARS2 protein of SARS CoV-<NUM> was produced recombinantly in E. coli strain BL21 (DE3) pDIA17 transformed with recombinant plasmid pETM11/N-nCov E. coli <NUM> - (His)<NUM>-Nter or pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter deposited at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, <NUM>, Rue du Docteur Roux, <NUM> Paris, FR, on May <NUM>, <NUM>, under the deposit numbers CNCM I-<NUM> and CNCM I-<NUM>, respectively. To achieve expression, DNA encoding the N_SARS2 was codon optimized for expression in bacteria and modified to express a His<NUM> N-terminal label. The DNA was transformed into E. coli and the protein produced was purified using a nickel column.

An indirect ELISA was used to compare plates coated with N_SARS2 to plates coated with N_SARS1 using serum from COVID-<NUM> patients with confirmed infections, as well as from various symptomatic patients (untested for SARS CoV-<NUM> RNA) and presumptively negative patients. Both N_SARS2 and N_SARS1 were able to detect anti- SARS CoV-<NUM> antibodies in the serum of all of COVID-<NUM> patients with confirmed infections, as well as in the serum of many of the symptomatic patients (untested for SARS CoV-<NUM> RNA). Anti- SARS CoV-2antibodies were not detected in the serum of presumptively negative patients. The difference between the two proteins was an increased sensitivity with the N_SARS2 protein (about <NUM>%). These results were surprising.

Rabbit hyper immune serum against N_SARS1 and N_SARS2 proteins was generated. Rabbit hyper immune serum against N_SARS1 and N_SARS2 proteins could bind to both the N_SARS1 and N_SARS2 proteins.

Alpaca were immunized with N_SARS2. Binding of plasma and serum VHH from the immunized alpaca to N_SARS2 protein was also detected.

These results allow for the use of the N_SARS2 protein or the N_SARS1 and N_SARS2 proteins for the detection of SARS-CoV and SARS CoV-<NUM> via immunoassay, as defined in the claims. The high correlation of immunoassay tests with N_SARS1 and N_SARS2 proteins with low cross-reactivity and high sensitivity indicates that these proteins can be used for immunodiagnostics of patients with of SARS-CoV and SARS CoV-<NUM>. The invention provides methods and kits containing said N_SARS2 protein produced from expression vectors or said N_SARS1 and N_SARS2 proteins according to the present disclosure.

Herein disclosed is a recombinant vector for expression of N protein of SARS-CoV or of N protein of SARS-CoV-<NUM>. The recombinant vector can be a vector for eukaryotic or prokaryotic expression, such as a plasmid, a phage for bacterium introduction, a YAC able to transform yeast, a viral vector and especially a retroviral vector, or any expression vector. An expression vector as defined herein is chosen to enable the production of an N protein or polyepitope, either in vitro or in vivo.

The expression vector expressing the N_SARS1 protein according to the present disclosure may beone of the expression vectors described in <CIT>.

For example, the recombinant vector expressing the N_SARS1 protein comprises an N cDNA cloned into the Expression Vector pIVEX2. <NUM> or pIVEX2. <NUM>, as described in <CIT>.

For example, the expression vector is pIV2.3N, particularly as contained in the bacteria transformed with plV2.3N. These bacteria pIV2.3N/DH5|alpha| were deposited under the terms of the Budapest Treaty at the Collection Nationale de Culture de Microorganismes (CNCM) on Oct. <NUM>, <NUM>, under the number I-<NUM>.

The recombinant vector for expression of N-SARS2 is pETM11/N-nCov E. coli <NUM> - (His)<NUM>-Nter as contained in the bacteria strain Bacterium_N-Cov_Ecoli_PETM11_coli3 deposited at the CNCM on May <NUM>, <NUM>, under the number I-<NUM>. or is pETM11/N-nCov E. coli <NUM>-(HIS)<NUM>-Nter as contained in the bacteria strain Bacterium_N-Cov_Ecoli_PETM11_coli4 deposited at the CNCM on May <NUM>, <NUM>, under the number I-<NUM>.

The expression vector according to the present disclosure may encode a protease cleavage site, such as TEV cleavage site, inserted between N protein coding sequence and a protein purification Tag, such as polyHis tag. For example, the expression vector encodes a His tag. For example, a protease cleavage site is positioned to remove the His tag, for example, after purification.

The expression vector according to the present disclosure can comprise transcription regulation regions (including promoter, enhancer, ribosome binding site (RBS), polyA signal), a termination signal, a prokaryotic or eukaryotic origin of replication and/or a selection gene. The features of the promoter can be easily determined by the man skilled in the art in view of the expression needed, i.e., constitutive, transitory or inducible (e.g. IPTG), strong or weak, tissue-specific and/or developmental stage-specific promoter. The vector can also comprise sequence enabling conditional expression, such as sequences of the Cre/Lox system or analogue systems.

The expression vector according to the present disclosure may be a plasmid, a phage for bacterium introduction, a YAC able to transform yeast, a viral vector, or any expression vector. An expression vector as defined herein is chosen to enable the production of a protein or polyepitope, either in vitro or in vivo.

The nucleic acid molecules according to the present disclosure can be obtained by conventional methods, known per se, following standard protocols such as those described in <NPL>). For example, they may be obtained by amplification of a nucleic sequence by PCR or RT-PCR or alternatively by total or partial chemical synthesis.

The vectors according to the present disclosure are constructed and introduced into host cells by conventional recombinant DNA and genetic engineering methods which are known per se. Numerous vectors into which a nucleic acid molecule of interest may be inserted in order to introduce it and to maintain it in a host cell are known per se; the choice of an appropriate vector depends on the use envisaged for this vector (for example replication of the sequence of interest, expression of this sequence, maintenance of the sequence in extrachromosomal form or alternatively integration into the chromosomal material of the host), and on the nature of the host cell.

Preferably, the N_SARS2 protein according to the present disclosure comprises the amino acid sequence of the SARS CoV-<NUM> protein of GenBank/NCBI accession number QHO62884. <NUM> accessed on July <NUM>, <NUM>.

Preferably, the N_SARS1 protein according to the present disclosure comprises the amino acid sequence of the SARS-CoV N protein of GenBank/NCBI accession number NP_828858. <NUM> accessed on June <NUM>, <NUM> or YP_009825061. <NUM> accessed on July <NUM>, <NUM>.

Preferably, the N_SARS2 protein according to the present disclosure comprises the following amino acid sequence. <IMG>
<IMG>.

Preferably, the N_SARS1 protein according to the present disclosure is one of the N proteins described in <CIT>, particularly SEQ ID NO:<NUM>.

In one embodiment, the N_SARS1 protein according to the present disclosure is produced by bacteria pIV2.3N/DH5|alpha| transformed with plV2.3N, which were deposited under the terms of the Budapest Treaty at the Collection Nationale de Culture de Microorganismes (CNCM) on Oct. <NUM>, <NUM>, under the number I-<NUM>. The address of CNCM is: Collection Nationale de Culture de Microorganismes, Institut Pasteur, <NUM> rue du Dr Roux, <NUM> Paris CEDEX <NUM>, France.

The N_SARS2 protein according to the present disclosure is produced by bacteria E. coli strain BL21 (DE3) pDIA17 transformed with the expression vector pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter or with the expression vector pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter. These two strains (Bacterium_N-Cov_Ecoli_PETM11_coli3 and Bacterium_N-Cov_Ecoli_PETM11_coli4) were deposited under the terms of the Budapest Treaty at the Collection Nationale de Culture de Microorganismes (CNCM) on May <NUM>, <NUM>, under the numbers I-<NUM> and I-<NUM> respectively.

In one embodiment the expression vector according to the present disclosure is contained within one of the following bacterial strains:.

These strains were deposited under the terms of the Budapest Treaty at the Collection Nationale de Culture de Microorganismes (CNCM) on Oct. <NUM>, <NUM>, under the numbers I-<NUM>, I-<NUM>, <NUM>-<NUM>, and I-<NUM> on <NUM>/<NUM>/<NUM> and I-<NUM> on <NUM>/<NUM>/<NUM>.

The disclosure encompasses "isolated or purified" N_SARS1 & N_SARS2 proteins. The terms "isolated or purified" mean modified "by the hand of humans" from the natural state; in other words if an object exists in nature, it is said to be isolated or purified if it is modified or extracted from its natural environment or both. For example, a polynucleotide or a protein/peptide naturally present in a living organism is neither isolated nor purified; on the other hand, the same polynucleotide or protein/peptide separated from coexisting molecules in its natural environment, obtained by cloning, amplification and/or chemical synthesis is isolated for the purposes of the present disclosure. Furthermore, a polynucleotide or a protein/peptide which is introduced into an organism by transformation, genetic manipulation or by any other method, is "isolated" even if it is present in said organism. The term purified as used in the present disclosure means that the proteins/peptides according to the disclosure are essentially free of association with the other proteins or polypeptides, as is for example the product purified from the culture of recombinant host cells or the product purified from a non-recombinant source. Various techniques can be used to obtain purified protein according to the disclosure as for example metal chelate binding chromatography and gel filtration.

Production of the N_SARS1 & N_SARS2 proteins can be achieved by any technique known to the skilled artisan, for example, as detailed in the examples or as described in in <CIT>.

In one embodiment, a method for producing SARS-CoV-<NUM> N protein comprises the following steps:.

Preferably, inducing the production of SARS-CoV-<NUM> N protein is performed by adding IPTG.

Preferably, recovering the SARS-CoV-<NUM> N protein is performed by pelleting and breakage of bacteria (bacteria cells). More preferably, recovering the SARS-CoV-<NUM> N protein is performed by pelleting and breakage of bacteria, and further recovering the soluble fraction of broken bacteria.

Preferably, purifying SARS-CoV-<NUM> N protein is performed by metal chelate affinity chromatography, and/or gel filtration.

Preferably, the SARS-CoV-<NUM> N protein made by the method disclosed herein is soluble.

The N_SARS2 protein or N_SARS2 and N_SARS1 proteins according to the present disclosure can be used for the detection and diagnosis of a SARS-associated coronavirus infection (serological diagnosis (detection of specific antibodies)), in particular by an immunoassay, such as an immunoenzymatic method (e.g., ELISA).

The invention is directed to methods for identifying a patient infected with a SARS-associated coronavirus, comprising providing a serum sample from the patient, contacting the serum with an N_SARS2 protein or N_SARS2 and N_SARS1 proteins, and visualizing the antigen-antibody complexes. Preferably, the antigen-antibody complexes are visualized by EIA, ELISA, RIA, or by immunofluorescence. In a preferred embodiment, the SARS-associated coronavirus infection is identified as SARS CoV-<NUM> infection. In some embodiments, the patient has been shown to be infected by SARS-CoV or SARS CoV-<NUM> by a nucleic acid detection test, such as a PCR or other nucleic acid amplification test. In some embodiments, the patient has been identified as being infected with a SARS-associated coronavirus, but lacks detection of the virus by PCR or another nucleic acid amplification technique.

The invention is directed to a composition comprising an N_SARS2 protein according to the present disclosure or the use of an N_SARS2 protein according to the present disclosure for detection of antibodies against a SARS coronavirus and diagnosis of a SARS coronavirus infection, in a biological sample. Preferably the SARS coronavirus is a SARS CoV-<NUM> coronavirus.

The ability of N_SARS2 to bind with high affinity to antibodies directed against a SARS-CoV allows its use in such methods, particularly for diagnostics of a SARS-CoV infection. Preferably the SARS CoV is a SARS-CoV-<NUM> (SARS-CoV) coronavirus or SARS-CoV-<NUM> coronavirus.

The ability of N_SARS1 to bind with high affinity to anti-SARS CoV-<NUM> antibodies allows its use in such methods, particularly for diagnostics of a SARS CoV-<NUM> infection.

Preferably, the patient has been shown to be infected by SARS-CoV or SARS CoV-<NUM> by a nucleic acid detection test, such as a PCR or other nucleic acid amplification test.

In one embodiment of the detection and diagnosis methods according to the invention, the N_SARS2 protein or N_SARS1 and N_SARS2 proteins is attached to an appropriate support, in particular a microplate or a bead.

In one embodiment, the method comprises bringing a biological sample from a subject, preferably a human, infected with a SARS-CoV or SARS CoV-<NUM> coronavirus into contact with an N_SARS2 protein or N_SARS1 and N_SARS2 proteins according to the present disclosure, which is attached to an appropriate support, in particular a microplate or bead, to allow binding to occur; washing the support to remove unbound antibodies; adding a detection reagent that binds to the immunoglobulins bound to N_SARS2 protein or N_SARS1 and N_SARS2 proteins; and detecting the N_SARS2 protein or N_SARS1 and N_SARS2 protein-antibody complexes formed.

In one embodiment, the method for the detection of antibodies directed to a SARS-associated coronavirus in a biological sample comprises providing a N_SARS2 protein according to the present disclosure; providing a biological sample from a patient infected with a SARS-CoV coronavirus; contacting said N_SARS2 protein with said biological sample; and visualizing the antigen-antibody complexes formed. Preferably, the method comprises an ELISA. Preferably, the N_SARS2 protein comprises or consists of the sequence of SEQ ID NO:<NUM>. Preferably the patient is infected with a SARS-CoV-<NUM> coronavirus.

In one embodiment, the method for the detection of antibodies against a SARS-associated coronavirus in a biological sample comprises providing N_SARS2 and N_SARS1 proteins; providing a biological sample from a patient infected with a SARS CoV-<NUM> coronavirus; contacting said N_SARS2 and N_SARS1 proteins with said biological sample; and visualizing the antigen-antibody complexes formed. Preferably, the method comprises an ELISA.

Preferably, the protein-antibody complexes are detected with an antibody or an antibody fragment that binds to human immunoglobulins.

Preferably, the detection reagent comprises a label is selected from a chemiluminescent label, an enzyme label, a fluorescence label, and a radioactive (e.g., iodine) label. Most preferably, the detection reagent is a labeled antibody or antibody fragment that binds to human immunoglobulins.

Preferred labels include a fluorescent label, such as FITC, a chromophore label, an affinity-ligand label, an enzyme label, such as alkaline phosphatase, horseradish peroxidase, luciferase or β galactosidase, an enzyme cofactor label, a hapten conjugate label, such as digoxigenin or dinitrophenyl, a Raman signal generating label, a magnetic label, a spin label, an epitope label, such as the FLAG or HA epitope, a luminescent label, a heavy atom label, a nanoparticle label, an electrochemical label, a light scattering label, a spherical shell label, semiconductor nanocrystal label, wherein the label can allow visualization with or without a secondary detection molecule.

Preferred labels include suitable enzymes such as horseradish peroxidase, alkaline phosphatase, beta-galactosidase, luciferase or acetylcholinesterase; members of a binding pair that are capable of forming complexes such as streptavidin/biotin, avidin/biotin or an antigen/antibody complex including, for example, rabbit IgG and anti-rabbit IgG; fluorophores such as umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, tetramethyl rhodamine, eosin, green fluorescent protein, erythrosin, coumarin, methyl coumarin, pyrene, malachite green, stilbene, lucifer yellow, Cascade Blue, Texas Red, dichlorotriazinylamine fluorescein, dansyl chloride, phycoerythrin, fluorescent lanthanide complexes such as those including Europium and Terbium, cyanine dye family members, such as Cy3 and Cy5, molecular beacons and fluorescent derivatives thereof, as well as others known in the art; a luminescent material such as luminol; light scattering or plasmon resonant materials such as gold or silver particles or quantum dots; or radioactive material include <NUM>C, <NUM>I, <NUM>I, <NUM>I, <NUM>P, <NUM>P, <NUM>S, or<NUM>H.

In one embodiment of the detection and diagnosis methods according to the invention,, the antibody or an antibody fragment that binds to human immunoglobulins binds specifically to IgG, IgA, and IgM. In one embodiment, the antibody or an antibody fragment that binds to human immunoglobulins binds specifically to IgG, IgA, or IgM.

The term "antibodies" is meant to include polyclonal antibodies, monoclonal antibodies, fragments thereof, such as F(ab')<NUM> and Fab fragments, single-chain variable fragments (scFvs), single-domain antibody fragments (VHHs or Nanobodies), bivalent antibody fragments (diabodies), as well as any recombinantly and synthetically produced binding partners.

In a preferred embodiment of the detection and diagnosis methods according to the invention, the antibody is a VHH, preferably an alpaca serum.

Preferably, the method comprises comparing the results obtained with a patient serum to positive and negative controls.

The method can comprise the use of N_SARS2 protein or N_SARS2 and N_SARS1 proteins to detect novel coronaviruses that do not cross-react with seasonal (non-pathogenic ) coronaviruses.

Preferably, the N_SARS2 protein comprises the amino acid sequence of the SARS CoV-<NUM> N protein of GenBank/NCBI accession number QHO62884. <NUM> accessed on July <NUM>, <NUM>.

Preferably, the N_SARS1 protein comprises the amino acid sequence of the SARS-CoV N protein of GenBank/NCBI accession number NP_828858. <NUM> accessed on June <NUM>, <NUM> or YP_009825061. <NUM> accessed on July <NUM>, <NUM>,.

In one embodiment of the detection and diagnosis methods according to the invention, the N_SARS2 protein comprises or consists of the amino acid sequence of SEQ ID NO:<NUM>.

In one embodiment of the detection and diagnosis methods according to the invention, the N_SARS1 protein is one of the N proteins described in <CIT>, particularly that of SEQ ID NO:<NUM>.

A visualizing molecule may be a radioactive atom, a dye, a fluorescent molecule, a fluorophore, an enzyme; a visualizing particle may be for example: colloidal gold, a magnetic particle or a latex bead.

The subject of the present invention is also a method for the detection of a SARS-associated coronavirus infection, from a biological sample, by indirect IgG ELISA using the N_SARS2 protein or N_SARS2 and N_SARS1 proteins according to the present disclosure, which method is characterized in that the plates are sensitized with an N_SARS2 protein or N_SARS1 and N_SARS2 proteins solution at a concentration of between <NUM> and <NUM>µg/ml, preferably to <NUM>µg/ml, in a <NUM> PBS buffer pH <NUM>, phenol red at <NUM>/l.

In one embodiment, microtiter plates are coated by incubation overnight at <NUM> with <NUM>µg/ml of N proteins. Plates are washed with <NUM>% Tween <NUM> in PBS buffer. Serum or purified Igs are diluted in PBS containing <NUM>% gelatin and <NUM>% Tween. After <NUM> incubation at <NUM>, plates are washed again. The bound antibodies are detected by adding a rabbit polyclonal anti-IgGs (obtained by immunizing rabbits with IgGs isolated on protein A and protein G columns) followed by Alkaline Phosphatase labeled goat anti-rabbit immunoglobulins. Enzymatic activity is quantified using pNPP (para-NitroPhenylPhosphate, SigmaAldrich) substrate according to the manufacturer's protocol.

According to one variant of the tests for detecting SARS-associated coronaviruses, these tests combine an ELISA using the N protein according to the present disclosure, and another ELISA using the S protein.

The invention is directed to a SARS-associated coronavirus detection kit, characterized in that it comprises a N_SARS2 protein or N_SARS2 and N_SARS1 proteins, as described above.

Preferably, the N_SARS1 protein comprises the amino acid sequence of the SARS-CoV N protein of GenBank/NCBI accession number NP_828858. <NUM> accessed on June <NUM>, <NUM> or YP_009825061. <NUM> accessed on July <NUM>, <NUM>.

In one embodiment, the invention comprises a kit for the detection of a SARS-CoV coronavirus infection, which kit contains a N_SARS2 protein and reagents for detection of antigen-antibody complexes.

Preferably, the kit contains a serum of an animal immunized with N_SARS2 and/or N_SARS1 proteins.

Most preferably, the serum is a rabbit or alpaca serum from an animal immunized with N_SARS2 and/or N_SARS1 proteins.

Most preferably, the serum is a rabbit or alpaca serum from an animal immunized with N_SARS2.

Preferably, the kit of the invention comprises an N_SARS2 protein comprising the amino acid sequence of the SARS CoV-<NUM> N protein of GenBank/NCBI accession number QHO62884. <NUM> accessed on July <NUM>, <NUM>.

Preferably, the kit of the invention comprises an N_SARS1 protein comprising the amino acid sequence of the SARS-CoV N protein of GenBank/NCBI accession number NP_828858. <NUM> accessed on June <NUM>, <NUM> or YP_009825061. <NUM> accessed on July <NUM>, <NUM>.

Preferably, the kit of the invention comprises an N_SARS2 protein that comprises or consists of the amino acid sequence of SEQ ID NO:<NUM>.

Preferably, the kit of the invention comprises an N_SARS1 protein that is one of the N proteins described in <CIT>, particularly that of SEQ ID NO:<NUM>.

In one embodiment, the kit comprises both N_SARS1 and N_SARS2 proteins and an N_SARS1 (N1) and/or N_SARS2 (N2) immune serum.

In one embodiment, the kit is a Simple/Rapid test designed for use where a preliminary screening test result is required. The tests can be a test based on agglutination, immuno-dot, immuno-chromatographic and/or immuno-filtration techniques. Preferably, the test is quick and easy to perform, preferably from about <NUM> minutes to about <NUM> hours, and requires little or no additional equipment.

Preferably, the kit can be stored at room temperature for extended period of time.

SARS-CoV-<NUM> recombinant N protein (N_SARS1) was produced using E. coli strain BL21 (DE3) pDIA17 transformed with the expression vector plV2.3N deposited under at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, <NUM>, Rue du Docteur Roux, <NUM> Paris, FR, on October <NUM><NUM>, under the deposit number CNCM I-<NUM> and purified as previously disclosed in <CIT> (see in particular the protocol disclosed in example <NUM>).

cDNAs encoding the native nucleoprotein antigen (N_SARS2) from <NUM>-nCoV (SARS-CoV-<NUM>) was designed base on the Genbank MN908947 sequence publicly available from NBCBI on 20th January <NUM>. This sequence was then processed to generate an optimized nucleotide sequences for high expression in E coli. Optimization process includes codon adaptation, mRNA de novo synthesis and stability, transcription and translation efficiency. Bsa1 and Xho1/EcoR1/Not1 restriction sites were then added at the <NUM>' and <NUM>' ends, respectively, of the nucleotide sequences. The resulting optimised cDNA named "N-Ecoli optimized gene" was synthesized. The Bsa1-Xho1 fragment of the "N-Ecoli optimized gene" has been inserted into Nco1/Xho1-digested pETM-<NUM> vector and the resulting pETM11-Necoli_2019-nCoV (= pETM11/N-nCov E. coli) has been used to produce a fusion polypeptide between the SARS-CoV-<NUM> protein and a N-terminally located poly-histidine tag (<NUM> histidine), separated by a TEV cleavage site.

The resulting His6-N_2019-nCoV (N_SARS2) polypeptide has the sequence :
<IMG>.

Nucleoprotein coding sequences (WT-CoV-<NUM> SARS DNA and E. coli optimized CoV-<NUM> SARS DNA) are cloned into pETM11 vector (EMBL; <NPL>) or pIVEX2-<NUM> (Roche vector) vectors. The N-recombinant Nucleoprotein of CoV-<NUM>-SARS is produced in E. coli BL21 (DE3) pDIA17 as a fusion protein comprising an N- or C-terminal (His)<NUM> polyhistidine label. Concerning the production of N-recombinant Nucleoprotein with a (His)<NUM> N-terminal label, the following recombinant vectors are used for the transformation of E. coli strain BL21 (DE3) pDIA17 :.

coli strain BL21 (DE3) pDIA17 transformed with recombinant plasmid pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter or pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter (Bacterium_N-Cov_Ecoli_PETM11_coli3 and Bacterium_N-Cov_Ecoli_PETM11_coli4) were deposited at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, <NUM>, Rue du Docteur Roux, <NUM> Paris, FR, on May <NUM>, <NUM> , under the deposit numbers CNCM I-<NUM> and CNCM I-<NUM>, respectively. Cultures in Thomson flasks shaken in LB medium (IPTG induction) and NZytech medium (self-inducible) of E. coli BL21 (DE3) pDIA17 strains transformed by the pETM11 vector or by the pIVEX <NUM> vector.

The Thomson flasks are <NUM> notched flasks allowing cultures of <NUM> litre of medium to be aerated under good aeration conditions in stirrers.

The <NUM> strains of E. coli BL21 (DE3) pDIA17 transformed by the pETM11 vector (DMSO no. <NUM>, <NUM>, <NUM>, <NUM>) are spread on an agar LB Petri dish containing <NUM>µg/ml kanamycin and <NUM>µg/ml chloramphenicol. The <NUM> strains of E. coli BL21 (DE3) pDIA17 transformed by the vector pIVEX2. <NUM> (DMSO n° <NUM>, <NUM>) are spread on an agar LB Petri dish containing <NUM>µg/ml ampicillin and <NUM>µg/ml chloramphenicol. All plates of LB Agar Petri LB are incubated overnight at <NUM> in an oven.

From each of the <NUM> Petri LB agar plates are inoculated with a platinum handle, <NUM> pre-cultures of <NUM> of LB medium in <NUM> Thomson flasks (LB medium plus antibiotics appropriate to each recombinant vector pETM11 and pIVEX2. These pre-cultures are shaken at <NUM> rpm in a Multitron Infors shaker for <NUM> at <NUM>.

From the <NUM> LB pre-cultures of BL21 (DE3) pDIA17 strains transformed by the pETM11 vector (DMSO No. <NUM>, <NUM>, <NUM>, <NUM>) are seeded at an initial cell density equivalent to DOA600 = <NUM>, cultures of <NUM> of LB medium containing <NUM>µg/ml kanamycin and <NUM>µg/ml chloramphenicol.

From the <NUM> LB pre-cultures of BL21 (DE3) pDIA17 strains transformed by the pIVEX <NUM> vector (DMSO No. <NUM>, <NUM>) are seeded at an initial cell density equivalent to DOA600 = <NUM>, cultures of <NUM> of LB medium containing <NUM>µg/ml ampicillin and <NUM>µg/ml chloramphenicol.

All these cultures in LB medium are placed under agitation at <NUM> rpm and <NUM>. When the cell density, equivalent to DOA600 = <NUM> is reached the cultures are induced by addition of <NUM> IPTG and the temperature is maintained at <NUM>.

After <NUM> hours at <NUM> in the presence of the inducer the cultures are stopped. A <NUM> sample of each culture is centrifuged and will be used for analysis on SDS-Page of the total soluble and insoluble protein fractions. The remainder of each culture is centrifuged (<NUM> at <NUM> rpm) and the pellets stored at -<NUM>.

From the <NUM> LB pre-cultures of BL21 (DE3) pDIA17 strains transformed by the pETM11 vector (DMSO No. <NUM>, <NUM>, <NUM>, <NUM>) are seeded at an initial cell density equivalent to DOA600 = <NUM>, <NUM> cultures in NZytech (self-inducible) medium containing <NUM>µg/ml kanamycin and <NUM>µg/ml chloramphenicol.

From the <NUM> LB pre-cultures of BL21 (DE3) pDIA17 strains transformed by the pIVEX <NUM> vector (DMSO No. <NUM>, <NUM>) are seeded at an initial cell density equivalent to DOA600 = <NUM>, <NUM> cultures in NZytech (self-inducible) medium containing <NUM>µg/ml ampicillin and <NUM>µg/ml chloramphenicol.

Cultures in NZytech medium (self-inducible) are carried out at <NUM> with stirring at <NUM> rpm.

After <NUM> hours at <NUM>, the cultures are placed at <NUM>.

After <NUM> hours of culture at this temperature of <NUM>, the bacterial cultures are stopped. A <NUM> sample of each culture is centrifuged and will be used for analysis on SDS-Page of the total soluble and insoluble protein fractions. The remainder of each culture is centrifuged (<NUM> at <NUM> rpm) and the pellets stored at -<NUM>. Cultures in BioPod F200 microfermenters in high cell density HDM medium (IPTG induction) of E. coli BL21 (DE3) pDIA17 strains transformed by the pETM11 vector or by the pIVEX <NUM> vector:
The HDM medium is a complex culture medium developed by our Platform specifically designed for the large production of E. coli biomass in a bioreactor during batch culture. This buffered medium does not require a regulation of the pH value in culture.

Microfermenters are miniaturized bioreactors allowing to realize <NUM> cultures in high density medium (HDM medium). These micro-fermenters are equipped with mass flow meters and sinter allowing a very efficient micro-bubbling by air progressively enriched with oxygen according to the bacterial growth. These bioreactors are also equipped with Peltier system and PT1000 probe which allow a very reliable regulation of the growth temperature and fast passages from <NUM> to <NUM> during the induction phase. This system of miniaturized bioreactors is a tool for optimizing the culture conditions allowing with a high rate of reliability a scale-up of <NUM> cultures to larger volume reactors (<NUM> and <NUM> in our Platform).

The <NUM> strains of E. coli BL21 (DE3) pDIA17 transformed by the pETM11 vector (DMSO n° <NUM> and <NUM>) are spread on an agar LB Petri dish containing 50µg/ml kanamycin and 30µg/ml chloramphenicol.

The <NUM> strains of E. coli BL21 (DE3) pDIA17 transformed by the vector pIVEX2. <NUM> (DMSO no. <NUM>, <NUM>) are spread on an agar LB Petri dish containing 100µg/ml ampicillin and 30µg/ml chloramphenicol. All LB agar plates are incubated overnight at <NUM> in an oven.

About <NUM> of antibiotic-free LB medium is deposited on each of the agar plates. The bacterial mat of each LB plate is scraped off with a sterile rake. Each bacterial suspension collected is used to inoculate a micro-fermentor containing <NUM> of HDM medium plus antibiotics appropriate for E. coli BL21 (DE3) pDIA17 strains transformed by the recombinant pETM11 or pIVEX2. <NUM> vectors. The initial cell density of the bioreactors is equivalent to A600= <NUM> to <NUM>.

The cultures are grown at a temperature of <NUM>, and aeration is set at <NUM> VVM. When the cell density equivalent to DOA600 = <NUM> to <NUM> is reached, the temperature is lowered to <NUM> and IPTG (<NUM>) is added to the cultures.

After <NUM> hours of culture at <NUM> in the presence of the inducer, the bacterial cultures are stopped. A <NUM> sample of each culture is centrifuged and will be used for analysis on SDS-Page of the total soluble and insoluble protein fractions. The remainder of each culture is centrifuged (<NUM> at <NUM> rpm) and the pellets stored at -<NUM>.

Take the <NUM> pellet with <NUM> buffer A: <NUM> phosphate, <NUM> NaCl, <NUM> imidazole pH8 with <NUM> Roche EDTA free protease tablet and <NUM>µl benzonase in the blender/wait incubation at room temperature for approx.

- 1st STEP OF PURIFICATION: AFFINITY IMAC (AKTAPure): <NUM> column Nickel <NUM>.

▪ <NUM> New <NUM> Protino Ni-NTA column (Macherey Nagel) mounted on AKTA Pure (room temperature).

▪ Loading the <NUM> crude extract onto the <NUM> IMAC column at a rate of <NUM>/min with the AKTA pump.

▪ Histogram peak integration for protein quantity estimation
Peak of the fractions from A5 to C12, i.e. <NUM> at <NUM>,<NUM>/ml. Estimated quantity on unicorn162 mg
▪ Fraction analysis at this stage on SDS-Page (<FIG>).

DO280 = <NUM>. For <NUM>/l, the OD is <NUM> <NUM> / <NUM> = <NUM>/ml. Either for <NUM>: <NUM> x <NUM>/ml = <NUM> total
The elution volume will be injected in <NUM> x <NUM> on <NUM> gel filtration columns with <NUM> loops. The columns are installed on the <NUM> pure AKTAs. <NUM> runs of gel filtration will be performed per column.

Equilibration of the columns with <NUM> Phosphate buffer, <NUM> NaCl pH8 Flow rate <NUM>/min.

N_SARS2 purified protein (concentration <NUM>/ml in phosphate <NUM> NaCl <NUM> pH8). Pool gel filtration <NUM> split into <NUM> parts : <NUM> filtered without antiproteases <NUM> aliquots of <NUM> stored at +<NUM> (box in cold room; <NUM> filtered with antiproteases <NUM> aliquots of <NUM> stored at +<NUM> (box in cold room); <NUM> unfiltered <NUM> aliquots of <NUM>,<NUM> stored at -<NUM> (box in freezer room); <NUM> unfiltered + glycerol <NUM>%final <NUM> aliquots of <NUM> stored at -<NUM> (box in freezer room).

coli optimized CoV-<NUM> SARS DNA cloned into pETM-<NUM> expression vector gave highest protein production yields in E. Unexpectedly, the clones pETM11/N-nCov E. coli <NUM> - (His)<NUM>-Nter and pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter were able to achieve high level production without protein aggregation.

The preparation of monospecific polyclonal antibodies directed against SARS-CoV-<NUM> recombinant N protein (N1 protein) has been disclosed previously in <CIT> (see in particular the rabbit immunization protocol disclosed in example <NUM>). Monospecific polyclonal antibodies directed against SARS-CoV-<NUM> recombinant N protein (N2 protein) were prepared in alpaca (Lama pacos) according to the following protocol. Monospecific polyclonal antibodies directed against SARS-CoV-<NUM> and SARS-CoV-<NUM> recombinant N proteins are useful as positive controls in SARS-CoV immunodiagnostic assays.

125µg of SARS-CoV-<NUM> recombinant Nucleoprotein (N) prepared according to example <NUM>, in a total volume of <NUM>µl was mixed with <NUM>µl of Freund complete adjuvant for the first immunization, and with <NUM>µl of Freund incomplete adjuvant for the following immunizations. One young adult alpaca (Lama pacos) named ANU was immunized at days <NUM>, <NUM> and <NUM> with the immunogen. The alpaca was bled at day <NUM>. The immune response was monitored by titration of serum and plasma samples by ELISA on coated Nucleoprotein as disclosed below.

Five mgs of SARS-CoV-<NUM> recombinant N protein were bound on Cyanogen Bromide-activated sepharose 4B (GE Healthcare Life science) according to the manufacturer's instructions. Twenty ml of plasma were incubated with the beads for 2hours, the beads were washed extensively with PBS until the OD280 =<NUM>. Then Glycine/HCl O. <NUM> pH <NUM> was added and the OD280 was monitored. Each fractions of <NUM> were neutralized with 100µl of Tris <NUM>. The positive fractions were pooled and dialysed against PBS. An ELISA was performed by coating the Nucleoprotein as disclosed below.

Microtiter plates were coated by incubation overnight at <NUM> with <NUM>µg/ml of SARS-CoV-<NUM> recombinant N protein. Plates were washed with <NUM>% Tween <NUM> in PBS buffer. Serum or purified Igs were diluted in PBS containing <NUM>% gelatin and <NUM>% Tween. After <NUM> incubation at <NUM>, plates were washed again. The bound alpaca antibodies were detected by adding a rabbit polyclonal anti-alpaca IgGs (obtained by immunizing rabbits with alpaca IgGs isolated on protein A and protein G columns) followed by Alkaline Phosphatase labeled goat anti-rabbit immunoglobulins. Enzymatic activity was quantified using pNPP (para-NitroPhenylPhosphate, SigmaAldrich) substrate according to the manufacturer's protocol.

<FIG> shows the binding of serum and plasma of alpaca ANU and <FIG> the binding of purified anti-N2 polyclonal antibodies on SARS-CoV-<NUM> nucleoprotein (N2 protein). The titer of the serum is very high (about <NUM>/<NUM>) and as low as <NUM>µg of polyclonal Ig can detect the bound SARS-CoV-<NUM> nucleoprotein. The lecture was performed after <NUM>.

Plates are coated with <NUM>µl of SARS-CoV-<NUM> N protein at <NUM>µg/ml or SARS-CoV-<NUM> N protein at <NUM>µg/ml on PBS1X over-night at <NUM>. Plates are washed <NUM> times with <NUM>µl PBS Tween <NUM>,<NUM>% (PBST), blocked with <NUM>µl PBST <NUM>% fat-free milk (PBSTL) for <NUM> at <NUM> and blocking solution is removed. 50µl of patient test serum diluted in PBSTL, at varying dilutions (<NUM>/<NUM> generally) is then added and incubated for 1hr at <NUM>. Plates are washed <NUM> times with <NUM>µl PBST. <NUM>µl peroxidase-conjugated anti-human Ig (Ig total, IgG specific, IgM specific or IgA specific) diluted according to manufacturer's instructions in PBSTL is then added and incubated for <NUM> at <NUM>. Plates are washed <NUM> times with <NUM>µl PBST. <NUM>µl TMB substrate prepared according to manufacturer's instructions is then added and incubated for <NUM> minutes RT in the dark. <NUM>µl H3PO4 are then added on the plate are read at <NUM> and <NUM>.

Human serum samples were tested in indirect ELISA using SARS-CoV-<NUM> N protein compared to SARS-CoV-<NUM> N protein. Tested samples were from the following groups:.

Various serum dilutions were tested (<NUM>/<NUM>; <NUM>/<NUM>; <NUM>/<NUM>; <NUM>/<NUM>). The results presented in <FIG> show that both SARS-CoV-<NUM> N and SARS-CoV-<NUM> N can detect SARS-CoV <NUM> infection with good sensitivity and specificity in patients with mild or severe SARS CoV-<NUM> infection. SARS-CoV-<NUM> N which has already been confirmed to have no cross reactivity with other seasonal coronaviruses is unexpectedly able to cross react with serum of patients with mild or severe SARS-CoV-<NUM> (COVID) infection.

The robustness of the indirect ELISA assay was confirmed using SARS-CoV-<NUM> N protein on cohorts of <NUM> COVID patients and <NUM> negative individuals (Table <NUM>).

The indirect ELISA assays allows the detection of <NUM> % of the COVID patients with a good specificity.

Rabbit hyperimmune monospecific polyclonal antibodies directed against SARS-CoV-<NUM> virions were tested in the indirect ELISA assay disclosed in example <NUM> using <NUM>µg/ml, <NUM>µg/ml or <NUM>µg/ml of SARS-CoV-<NUM> or <NUM>µg/ml of SARS-CoV-<NUM> recombinant N protein. The results presented in <FIG> shows that SARS-CoV-<NUM> N is unexpectedly able to cross react with anti SARS-CoV-<NUM> antibodies and can detect anti SARS-CoV-<NUM> antibodies with a good sensitivity and a good specificity.

White <NUM>- or <NUM>-well plates with flat bottom (Fluoronunc C96 Maxisorp, Nunc) were coated by adsorption with either <NUM>µg/mL of Nucleoprotein produced as in Example <NUM>, or Spike protein, in <NUM>µL/well of phosphate buffer saline pH <NUM> (Sigma) for <NUM> hours at room temperature or overnight at <NUM>. Adsorption on Maxisorp® plates favours charge interaction. Alternatives to adsorption are covalent binding of targets through carboxylic, amine or sulfhydryl moieties or glycosylation at the functionalized well surface or coating with poly-lysine. Wells were emptied eventually but not necessarily neutralize with BSA (<NUM>/mL) or skimmed milk <NUM>%. Wells were washed <NUM> to <NUM> times with <NUM>µL of PBS/Tween <NUM><NUM>%. Dilutions of serum (typically <NUM>/<NUM>), plasma or body fluid were incubated from <NUM> to <NUM> hour at room temperature in their respective antigen-coated wells, <NUM>µL/well in phosphate buffer saline with eventually skimmed milk <NUM>%, bovine serum albumin <NUM>/mL, bovine serum <NUM>-<NUM>% and/or Tween <NUM><NUM>%. Wells were washed three to six times with <NUM>µL PBS/Tween <NUM><NUM>%. Purified anti-IgG nanobody-nanoKAZ fusion protein at <NUM> ng/mL (<NUM> RLU. mL-<NUM>) in phosphate buffer saline with eventually skimmed milk <NUM>%, bovine serum albumin <NUM>/mL or bovine serum <NUM>-<NUM>% and/or Tween <NUM><NUM>%, was loaded (<NUM>µL/well) and incubated <NUM>-<NUM> at room temperature. Wells were washed four times with <NUM>µL of PBS/Tween <NUM><NUM>%. Plates can be stored at this step in PBS until measurements. Just before reading, each wells were emptied and loaded with <NUM>µL of furimazine (<NUM>-benzyl-<NUM>-(furan-<NUM>-ylmethyl)-<NUM>-phenylimidazo[<NUM>,<NUM>-a]pyrazin-<NUM>(<NUM>)-one) at <NUM>. The plate was orbitally shaked for <NUM> seconds and the light emission intensity was integrated <NUM>-<NUM> sec per well using a multi-well plate luminometer (LB960 Centro, Berthold).

IgGs specific of protein N of SARS-CoV-<NUM> have been titrated by luciferase linked immunosorbent assay in serums of the CORSER cohort (n=<NUM> ; IcareB), in serums of the observation and monitoring protocol of the Intensive Medicine and Resuscitation service of Assistance Publique des Hôpitaux de Paris (APHP) Cochin (n=<NUM>) in longitudinal follow-up and in serums and plasma of healthy donors of Etablissement Français du Sang (EFS) sampled in December <NUM> (frozen plasmas n=<NUM>, frozen serums n=<NUM>). Results are shown in <FIG>.

IgGs specific of protein Spike (S) of SARS-CoV-<NUM> have been titrated in serums from the observation and monitoring protocol of the Intensive Medicine and Resuscitation service of Assistance Publique des Hôpitaux de Paris (APHP) Cochin (n=<NUM>) and in frozen serums from healthy donors of EFS (n=<NUM>). Results are shown in <FIG>.

The correlation between the titration of IgG specific of protein N of SARS-CoV-<NUM> and IgG specific of protein S of SARS-CoV-<NUM> in serum from APHP-Cochin (n=<NUM>) and EFS (n=<NUM>) is shown in <FIG>.

Then it was compared the IgG of serums from patients (APHP-Cochin n=<NUM>) and frozen serums of healthy donors (EFS n=<NUM>) which were specific for SARS-CoV-<NUM> N protein and SARS CoV-<NUM> N protein. Results are shown in <FIG>.

Claim 1:
A method for the detection of a severe acute respiratory syndrome (SARS)-associated coronavirus infection in a biological sample comprising:
- providing a severe acute respiratory syndrome-associated coronavirus <NUM> (SARS-CoV-<NUM>) nucleocapsid (N) protein expressed and purified from E. coli strain BL21 (DE3) pDIA17 transformed with recombinant plasmid pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter or pETM11/N-nCov E. coli <NUM> -(His)<NUM>-Nter deposited at the Collection Nationale de Cultures de Microorganismes (CNCM) at the Institut Pasteur, <NUM>, Rue du Docteur Roux, <NUM> Paris, FR, on May <NUM>, <NUM>, under the deposit numbers CNCM I-<NUM> and CNCM I-<NUM>, respectively;
- providing a biological sample from an individual or a patient suspected to be infected with a severe acute respiratory syndrome-associated coronavirus (SARS-CoV);
- contacting said SARS-CoV-<NUM> N protein with said biological sample; and
- visualizing the antigen-antibody complexes formed.