Patent Description:
In the continuing interest of synthesizing bioactive natural products, and taking into consideration the novel structure of the callyspongiolide macrolide, the inventors sought to establish a convergent and enantioselective synthesis of callyspongiolide to aid in structural determination and to further probe biological studies of not only callyspongiolide, but also analogs of the compound.

Reference will now be made in detail to certain examples of the disclosed subject matter, examples of which are illustrated in part in the accompanying drawings. While the disclosed subject matter will be described in conjunction with the enumerated claims, it will be understood that the exemplified subject matter is not intended to limit the claims to the disclosed subject matter.

A compound of the formula (I) and (II) is shown herein but not claimed:
<CHM>
<CHM>.

The invention is directed to a compound of the formula (III), or a pharmaceutically acceptable salt, polymorph, solvate or clathrate thereof, as well as methods of making and using compounds of the formula (III) to, among other things, treat various forms of cancer:
<CHM>
wherein:.

In some examples, the compound of the formula (III) is a compound of the formula:
<CHM>
provided the compound is not of formula (I).

Each R<NUM> is, independently, C<NUM>-C<NUM> alkyl (e.g., methyl, ethyl or propyl). In other examples, each R<NUM> is, independently, halo or OR<NUM>. In still other examples, each R<NUM> is, independently, C<NUM>-C<NUM> alkyl. And in other examples, R<NUM> is OR<NUM>.

A compound of the formula (IV) is:
<CHM>.

A compound of the formula (V) is:
<CHM>
wherein the substituents R<NUM>-R<NUM> and X<NUM>-X<NUM> are defined herein.

For reference (and not claimed), the compound of the formula (IV) and (V) can be synthesized from a compound of the formula (VI) and (VII), respectively:
<CHM>
<CHM>
wherein the substituents R<NUM>-R<NUM> and X<NUM>-X<NUM> are defined herein and P<NUM> and P<NUM> are, independently, a suitable oxygen protecting group. See <NPL>) for other commonly-used protecting groups for hydroxyl groups. It should be understood that the compound of formula (VI) can be modified to access compounds where X<NUM> is other than O by using, for example, a compound where the OP<NUM> group is replaced by a NR<NUM>P<NUM> group, wherein P<NUM> is a suitable amine protecting group.

For reference (and not claimed), the compounds of the formula (VI) and (VII) can be synthesized from a compound of the formula (VIII) and (IX), respectively:
<CHM>
<CHM>
wherein the substituents R<NUM>-R<NUM> and X<NUM>-X<NUM> are defined herein and P<NUM> and P<NUM> are, independently, a suitable oxygen protecting group. See<NPL>) for other commonly-used protecting groups for hydroxyl groups. It should be understood that the compound of formula (VIII) can be modified to access compounds where X<NUM> is other than O by using, for example, a compound where the OP<NUM> group is replaced by a NR<NUM>P<NUM> group, wherein P<NUM> is a suitable amine protecting group. In addition, it should be understood that one or more groups R<NUM> can also be on the ring comprising X<NUM>.

A compound of the formula (XI) is:
<CHM>.

The invention provides methods of making a compound of the formula (III) with the compound of the formula (X) and a compound of the formula (XI) to give a compound of the formula (III):
<CHM>
which method is defined in the claims.

Those of ordinary skill in the art will recognize that compounds described herein can contain chiral centers. All diastereomers of the compounds described herein are contemplated herein, as well as racemates.

Various examples of the present invention also contemplate pharmaceutical compositions comprising one or more compounds of the various examples of the present invention (e.g., compounds of the formula (III)) and one or more pharmaceutically acceptable carriers, diluents, excipients or combinations thereof. A "pharmaceutical composition" refers to a chemical or biological composition suitable for administration to a subject (e.g., mammal). Such compositions may be specifically formulated for administration via one or more of a number of routes, including but not limited to buccal, cutaneous, epicutaneous, epidural, infusion, inhalation, intraarterial, intracardial, intracerebroventricular, intradermal, intramuscular, intranasal, intraocular, intraperitoneal, intraspinal, intrathecal, intravenous, oral, parenteral, pulmonary, rectally via an enema or suppository, subcutaneous, subdermal, sublingual, transdermal, and transmucosal. In addition, administration can by means of capsule, drops, foams, gel, gum, injection, liquid, patch, pill, porous pouch, powder, tablet, or other suitable means of administration.

A "pharmaceutical excipient" or a "pharmaceutically acceptable excipient" comprises a carrier, sometimes a liquid, in which an active therapeutic agent is formulated. The excipient generally does not provide any pharmacological activity to the formulation, though it may provide chemical and/or biological stability, and release characteristics. Examples of suitable formulations can be found, for example, in<NPL>or), <NPL>, which is incorporated by reference in its entirety.

As used herein "pharmaceutically acceptable carrier" or "excipient" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents that are physiologically compatible. In one example, the carrier is suitable for parenteral administration. Alternatively, the carrier can be suitable for intravenous, intraperitoneal, intramuscular, sublingual, or oral administration. Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the pharmaceutical compositions of the invention is contemplated. Supplementary active compounds can also be incorporated into the compositions.

Pharmaceutical compositions may be sterile and stable under the conditions of manufacture and storage. The composition can be formulated as a solution, microemulsion, liposome, or other ordered structure suitable to high drug concentration. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol, and liquid polyethylene glycol), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants.

In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, or sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, monostearate salts and gelatin. Moreover, the compounds described herein can be formulated in a time release formulation, for example in a composition that includes a slow release polymer. The active compounds can be prepared with carriers that will protect the compound against rapid release, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers may be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, polylactic acid and polylactic, polyglycolic copolymers (PLG). Many methods for the preparation of such formulations are known to those skilled in the art.

Oral forms of administration are also contemplated herein. The pharmaceutical compositions of the present invention may be orally administered as a capsule (hard or soft), tablet (film coated, enteric coated or uncoated), powder or granules (coated or uncoated) or liquid (solution or suspension). The formulations may be conveniently prepared by any of the methods well-known in the art. The pharmaceutical compositions of the present invention may include one or more suitable production aids or excipients including fillers, binders, disintegrants, lubricants, diluents, flow agents, buffering agents, moistening agents, preservatives, colorants, sweeteners, flavors, and pharmaceutically compatible carriers.

For each of the recited examples, the compounds can be administered by a variety of dosage forms as known in the art. Any biologically-acceptable dosage form known to persons of ordinary skill in the art, and combinations thereof, are contemplated. Examples of such dosage forms include, without limitation, chewable tablets, quick dissolve tablets, effervescent tablets, reconstitutable powders, elixirs, liquids, solutions, suspensions, emulsions, tablets, multi-layer tablets, bi-layer tablets, capsules, soft gelatin capsules, hard gelatin capsules, caplets, lozenges, chewable lozenges, beads, powders, gum, granules, particles, microparticles, dispersible granules, cachets, douches, suppositories, creams, topicals, inhalants, aerosol inhalants, patches, particle inhalants, implants, depot implants, ingestibles, injectables (including subcutaneous, intramuscular, intravenous, and intradermal), infusions, and combinations thereof.

Other compounds which can be included by admixture are, for example, medically inert ingredients (e.g., solid and liquid diluent), such as lactose, dextrosesaccharose, cellulose, starch or calcium phosphate for tablets or capsules, olive oil or ethyl oleate for soft capsules and water or vegetable oil for suspensions or emulsions; lubricating agents such as silica, talc, stearic acid, magnesium or calcium stearate and/or polyethylene glycols; gelling agents such as colloidal clays; thickening agents such as gum tragacanth or sodium alginate, binding agents such as starches, arabic gums, gelatin, methylcellulose, carboxymethylcellulose or polyvinylpyrrolidone; disintegrating agents such as starch, alginic acid, alginates or sodium starch glycolate; effervescing mixtures; dyestuff; sweeteners; wetting agents such as lecithin, polysorbates or laurylsulphates; and other therapeutically acceptable accessory ingredients, such as humectants, preservatives, buffers and antioxidants, which are known additives for such formulations.

Liquid dispersions for oral administration can be syrups, emulsions, solutions, or suspensions. The syrups can contain as a carrier, for example, saccharose or saccharose with glycerol and/or mannitol and/or sorbitol. The suspensions and the emulsions can contain a carrier, for example a natural gum, agar, sodium alginate, pectin, methylcellulose, carboxymethylcellulose, or polyvinyl alcohol.

The amount of active compound in a therapeutic composition according to various examples of the present invention may vary according to factors such as the disease state, age, gender, weight, patient history, risk factors, predisposition to disease, administration route, pre-existing treatment regime (e.g., possible interactions with other medications), and weight of the individual. Dosage regimens may be adjusted to provide the optimum therapeutic response. For example, a single bolus may be administered, several divided doses may be administered over time, or the dose may be proportionally reduced or increased as indicated by the exigencies of therapeutic situation.

"Dosage unit form," as used herein, refers to physically discrete units suited as unitary dosages for the mammalian subjects to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of sensitivity in individuals. In therapeutic use for treatment of conditions in mammals (e.g., humans) for which the compounds of the present invention or an appropriate pharmaceutical composition thereof are effective, the compounds of the present invention may be administered in an effective amount. The dosages as suitable for this invention may be a composition, a pharmaceutical composition or any other compositions described herein.

For each of the recited examples, the dosage is typically administered once, twice, or thrice a day, although more frequent dosing intervals are possible. The dosage may be administered every day, every <NUM> days, every <NUM> days, every <NUM> days, every <NUM> days, every <NUM> days, and/or every <NUM> days (once a week). In one example, the dosage may be administered daily for up to and including <NUM> days, preferably between <NUM>-<NUM> days. In another example, the dosage may be administered twice a day for <NUM> days. If the patient requires treatment for a chronic disease or condition, the dosage may be administered for as long as signs and/or symptoms persist. The patient may require "maintenance treatment" where the patient is receiving dosages every day for months, years, or the remainder of their lives. In addition, the composition of this invention may be to effect prophylaxis of recurring symptoms. For example, the dosage may be administered once or twice a day to prevent the onset of symptoms in patients at risk, especially for asymptomatic patients.

The compositions described herein may be administered in any of the following routes: buccal, epicutaneous, epidural, infusion, inhalation, intraarterial, intracardial, intracerebroventricular, intradermal, intramuscular, intranasal, intraocular, intraperitoneal, intraspinal, intrathecal, intravenous, oral, parenteral, pulmonary, rectally via an enema or suppository, subcutaneous, subdermal, sublingual, transdermal, and transmucosal. The preferred routes of administration are buccal and oral. The administration can be local, where the composition is administered directly, close to, in the locality, near, at, about, or in the vicinity of, the site(s) of disease, e.g., inflammation, or systemic, wherein the composition is given to the patient and passes through the body widely, thereby reaching the site(s) of disease. Local administration can be administration to the cell, tissue, organ, and/or organ system, which encompasses and/or is affected by the disease, and/or where the disease signs and/or symptoms are active or are likely to occur. Administration can be topical with a local effect, composition is applied directly where its action is desired. Administration can be enteral wherein the desired effect is systemic (non-local), composition is given via the digestive tract. Administration can be parenteral, where the desired effect is systemic, composition is given by other routes than the digestive tract.

In some examples, the various examples of the present invention contemplate compositions comprising a therapeutically effective amount of one or more compounds of the various examples of the present invention. In some aspects, the various examples of the present invention contemplate a compound of the formulae (III) for use as a medicament for treating a patient in need of relief from cancer.

The term "therapeutically effective amount" as used herein, refers to that amount of one or more compounds of the various examples of the present invention that elicits a biological or medicinal response in a tissue system, animal or human, that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes alleviation of the symptoms of the disease or disorder being treated. In some examples, the therapeutically effective amount is that which may treat or alleviate the disease or symptoms of the disease at a reasonable benefit/risk ratio applicable to any medical treatment. However, it is to be understood that the total daily usage of the compounds and compositions described herein may be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically-effective dose level for any particular patient will depend upon a variety of factors, including the condition being treated and the severity of the condition; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, gender and diet of the patient: the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidentally with the specific compound employed; and like factors well known to the researcher, veterinarian, medical doctor or other clinician. It is also appreciated that the therapeutically effective amount can be selected with reference to any toxicity, or other undesirable side effect, that might occur during administration of one or more of the compounds described herein.

In some examples, the compounds of the various examples of the present invention have a half maximal inhibitory concentration (IC<NUM>) of from about <NUM> to about <NUM> (e.g., about <NUM> to about <NUM>, about <NUM> to about <NUM>, about <NUM> to about <NUM>, about <NUM> to about <NUM>, about <NUM> to about <NUM>, about <NUM> to about <NUM> or about <NUM> to about <NUM>).

Values expressed in a range format should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range were explicitly recited. For example, a range of "about <NUM>% to about <NUM>%" or "about <NUM>% to <NUM>%" should be interpreted to include not just about <NUM>% to about <NUM>%, but also the individual values (e.g., <NUM>%, <NUM>%, <NUM>%, and <NUM>%) and the sub-ranges (e.g., <NUM>% to <NUM>%, <NUM>% to <NUM>%, <NUM>% to <NUM>%) within the indicated range. The statement "about X to Y" has the same meaning as "about X to about Y," unless indicated otherwise. Likewise, the statement "about X, Y, or about Z" has the same meaning as "about X, about Y, or about Z," unless indicated otherwise.

In this document, the terms "a," "an," or "the" are used to include one or more than one unless the context clearly dictates otherwise. The term "or" is used to refer to a nonexclusive "or" unless otherwise indicated. In addition, it is to be understood that the phraseology or terminology employed herein, and not otherwise defined, is for the purpose of description only and not of limitation. Any use of section headings is intended to aid reading of the document and is not to be interpreted as limiting. Further, information that is relevant to a section heading may occur within or outside of that particular section. Furthermore, all publications, patents, and patent documents referred to in this document are provided for reference herein in their entirety.

In the methods described herein, the steps can be carried out in any order without departing from the principles of the invention, except when a temporal or operational sequence is explicitly recited. Furthermore, specified steps can be carried out concurrently unless explicit claim language recites that they be carried out separately. For example, a claimed step of doing X and a claimed step of doing Y can be conducted simultaneously within a single operation, and the resulting process will fall within the literal scope of the claimed process.

The term "about" as used herein can allow for a degree of variability in a value or range, for example, within <NUM>%, within <NUM>%, or within <NUM>% of a stated value or of a stated limit of a range.

The term "substituted" as used herein refers to a group (e.g., alkyl, aryl, and heteroaryl) or molecule in which one or more hydrogen atoms contained thereon are replaced by one or more substituents. The term "substituent" as used herein refers to a group that can be or is substituted onto a molecule or onto a group. Examples of substituents include, but are not limited to, a halogen (e.g., F, Cl, Br, and I); an oxygen atom in groups such as hydroxyl groups, alkoxy groups, aryloxy groups, aralkyloxy groups, oxo(carbonyl) groups, carboxyl groups including carboxylic acids, carboxylates, and carboxylate esters; a sulfur atom in groups such as thiol groups, alkyl and aryl sulfide groups, sulfoxide groups, sulfone groups, sulfonyl groups, and sulfonamide groups; a nitrogen atom in groups such as amines, hydroxylamines, nitriles, nitro groups, N-oxides, hydrazides, azides, and enamines; and other heteroatoms in various other groups. Non-limiting examples of substituents that can be bonded to a substituted carbon (or other) atom include F, Cl, Br, I, OR, OC(O)N(R)z, CN, NO, NO<NUM>, ONO<NUM>, azido, CF<NUM>, OCF<NUM>, R, O (oxo), S (thiono), C(O), S(O), methylenedioxy, ethylenedioxy, N(R)<NUM>, SR, SOR, SO<NUM>R, SO<NUM>N(R)<NUM>, SO<NUM>R, C(O)R, C(O)C(O)R, C(O)CH<NUM>C(O)R, C(S)R, C(O)OR, OC(O)R, C(O)N(R)<NUM>, OC(O)N(R)<NUM>, C(S)N(R)<NUM>, (CH<NUM>)<NUM>-<NUM>N(R)C(O)R, (CH<NUM>)<NUM>-<NUM>N(R)N(R)<NUM>, N(R)N(R)C(O)R, N(R)N(R)C(O)OR, N(R)N(R)CON(R)<NUM>, N(R)SO<NUM>R, N(R)SO<NUM>N(R)<NUM>, N(R)C(O)OR, N(R)C(O)R, N(R)C(S)R, N(R)C(O)N(R)<NUM>, N(R)C(S)N(R)<NUM>, N(COR)COR, N(OR)R, C(=NH)N(R)<NUM>, C(O)N(OR)R, or C(=NOR)R, wherein R can be, for example, hydrogen, alkyl, acyl, cycloalkyl, aryl, aralkyl, heterocyclyl, heteroaryl, or heteroarylalkyl.

The term "alkyl" as used herein refers to substituted or unsubstituted straight chain and branched alkyl groups and cycloalkyl groups having from <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>), <NUM> to about <NUM> carbon atoms (C<NUM>-C<NUM>), <NUM> to <NUM> carbons (C<NUM>-C<NUM>), <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>), or, in some examples, from <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>). Examples of straight chain alkyl groups include those with from <NUM> to <NUM> carbon atoms such as methyl, ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl, n-heptyl, and n-octyl groups. Examples of branched alkyl groups include, but are not limited to, isopropyl, iso-butyl, sec-butyl, t-butyl, neopentyl, isopentyl, and <NUM>,<NUM>-dimethylpropyl groups. Representative substituted alkyl groups can be substituted one or more times with any of the groups listed herein, for example, amino, hydroxy, cyano, carboxy, nitro, thio, alkoxy, and halogen groups.

The term "cycloalkyl" as used herein refers to substituted or unsubstituted cyclic alkyl groups such as, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl groups. In some examples, the cycloalkyl group can have <NUM> to about <NUM>-<NUM> ring members, whereas in other examples the number of ring carbon atoms range from <NUM> to <NUM>, <NUM>, <NUM>, or <NUM>. In some examples, cycloalkyl groups can have <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>). Cycloalkyl groups further include polycyclic cycloalkyl groups such as, but not limited to, norbornyl, adamantyl, bornyl, camphenyl, isocamphenyl, and carenyl groups, and fused rings such as, but not limited to, decalinyl.

The term "acyl" as used herein refers to a group containing a carbonyl moiety wherein the group is bonded via the carbonyl carbon atom. The carbonyl carbon atom is also bonded to another carbon atom, which can be part of a substituted or unsubstituted alkyl, aryl, aralkyl cycloalkyl, cycloalkylalkyl, heterocyclyl, heterocyclylalkyl, heteroaryl or heteroarylalkyl group. In the special case wherein the carbonyl carbon atom is bonded to a hydrogen, the group is a "formyl" group, an acyl group as the term is defined herein. An acyl group can include <NUM> to about <NUM>-<NUM>, <NUM>-<NUM>, <NUM>-<NUM> or <NUM>-<NUM> additional carbon atoms bonded to the carbonyl group. An acryloyl group is an example of an acyl group. An acyl group can also include heteroatoms within the meaning here. A nicotinoyl group (pyridyl-<NUM>-carbonyl) is an example of an acyl group within the meaning herein. Other examples include acetyl, benzoyl, phenylacetyl, pyridylacetyl, cinnamoyl, and acryloyl groups. When the group containing the carbon atom that is bonded to the carbonyl carbon atom contains a halogen, the group is termed a "haloacyl" group. An example is a trifluoroacetyl group.

The term "aryl" as used herein refers to substituted or unsubstituted cyclic aromatic hydrocarbons that do not contain heteroatoms in the ring. Thus aryl groups include, but are not limited to, phenyl, azulenyl, heptalenyl, biphenyl, indacenyl, fluorenyl, phenanthrenyl, triphenylenyl, pyrenyl, naphthacenyl, chrysenyl, biphenylenyl, anthracenyl, and naphthyl groups. In some examples, aryl groups contain about <NUM> to about <NUM> carbons (C<NUM>-C<NUM>) or from <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>) in the ring portions of the groups. Aryl groups can be unsubstituted or substituted, as defined herein. Representative substituted aryl groups can be mono-substituted or substituted more than once, such as, but not limited to, <NUM>-, <NUM>-, <NUM>-, <NUM>-, or <NUM>-substituted phenyl or <NUM>-<NUM> substituted naphthyl groups, which can be substituted with carbon or non-carbon groups such as those listed herein.

The term "aralkyl" and "arylalkyl" as used herein refers to alkyl groups as defined herein in which a hydrogen or carbon bond of an alkyl group is replaced with a bond to an aryl group as defined herein. Representative aralkyl groups include benzyl and phenylethyl groups.

The term "heterocyclyl" as used herein refers to substituted or unsubstituted aromatic and non-aromatic ring compounds containing <NUM> or more ring members, of which, one or more is a heteroatom such as, but not limited to, N, O, and S. Thus, a heterocyclyl can be a cycloheteroalkyl, or a heteroaryl, or if polycyclic, any combination thereof. In some examples, heterocyclyl groups include <NUM> to about <NUM> ring members, whereas other such groups have <NUM> to about <NUM> ring members. In some examples, heterocyclyl groups include heterocyclyl groups that include <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>), <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>), <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>), <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>) or <NUM> to <NUM> carbon atoms (C<NUM>-C<NUM>). A heterocyclyl group designated as a C<NUM>-heterocyclyl can be a <NUM>-ring with two carbon atoms and three heteroatoms, a <NUM>-ring with two carbon atoms and four heteroatoms and so forth. Likewise a C<NUM>-heterocyclyl can be a <NUM>-ring with one heteroatom, a <NUM>-ring with two heteroatoms, and so forth. The number of carbon atoms plus the number of heteroatoms equals the total number of ring atoms. A heterocyclyl ring can also include one or more double bonds. A heteroaryl ring is an example of a heterocyclyl group. The phrase "heterocyclyl group" includes fused ring species including those that include fused aromatic and non-aromatic groups. Representative heterocyclyl groups include, but are not limited to piperidynyl, piperazinyl, morpholinyl, furanyl, pyrrolidinyl, pyridinyl, pyrazinyl, pyrimidinyl, triazinyl, thiophenyl, tetrahydrofuranyl, pyrrolyl, oxazolyl, imidazolyl, triazyolyl, tetrazolyl, benzoxazolinyl, and benzimidazolinyl groups.

The term "alkoxy" as used herein refers to an oxygen atom connected to an alkyl group, including a cycloalkyl group, as are defined herein. Examples of linear alkoxy groups include but are not limited to methoxy, ethoxy, propoxy, butoxy, pentyloxy, and hexyloxy. Examples of branched alkoxy include but are not limited to isopropoxy, sec-butoxy, tert-butoxy, isopentyloxy, and isohexyloxy. Examples of cyclic alkoxy include but are not limited to cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, and cyclohexyloxy. An alkoxy group can include one to about <NUM>-<NUM> or about <NUM>-<NUM> carbon atoms bonded to the oxygen atom, and can further include double or triple bonds, and can also include heteroatoms. For example, an allyloxy group is an alkoxy group within the meaning herein. A methoxyethoxy group is also an alkoxy group within the meaning herein, as is a methylenedioxy group in a context where two adjacent atoms of a structure are substituted therewith.

The term "amine" as used herein refers to primary, secondary, and tertiary amines having, e.g., the formula N(group)<NUM> wherein each group can independently be H or non-H, such as alkyl, and aryl. Amines include but are not limited to alkylamines, arylamines, arylalkylamines; dialkylamines, diarylamines, diaralkylamines, and heterocyclylamines; and ammonium ions.

The terms "halo," "halogen," or "halide" group, as used herein, by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.

As used herein, the term "salts" and "pharmaceutically acceptable salts" refer to derivatives of the disclosed compounds wherein the parent compound is modified by making acid or base salts thereof. Examples of pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic groups such as amines; and alkali or organic salts of acidic groups such as carboxylic acids. Pharmaceutically acceptable salts include the conventional non-toxic salts or the quaternary ammonium salts of the parent compound formed, for example, from non-toxic inorganic or organic acids. For example, such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, and nitric; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, <NUM>-acetoxybenzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, and isethionic.

Pharmaceutically acceptable salts can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods. In some instances, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in <NPL>, the disclosure of which is hereby incorporated by reference.

The term "solvate" means a compound, or a salt thereof, that further includes a stoichiometric or non-stoichiometric amount of solvent bound by non-covalent intermolecular forces. Where the solvent is water, the solvate is a hydrate.

The present invention can be better understood by reference to the following examples which are offered by way of illustration. The present invention is not limited to the examples given herein and is defined in the appended claims.

The following syntheses and retrosyntheses of callyspongiolide are provided for reference only and are not claimed.

The retrosynthesis of callyspongiolide is outlined in Scheme <NUM>.

A late-stage Sonogashira coupling of vinyl iodide <NUM> with either enantiomer of enyne <NUM> would allow access to both antipodes of the proposed molecule. The macrolactone is constructed by macrolactonization of seco acid <NUM>, which is accessible via ring-opening of lactone <NUM>. The "congested" olefin of <NUM> would be formed through a modified Julia olefination using a sulfone derived from known diol <NUM> and an aldehyde obtained from known allyl alcohol <NUM>. Wittig olefination with the aldehyde derived from olefin <NUM> gives enyne <NUM>. Stereoselective Corey-Bakshi-Shibata (CBS) reduction of a hindered ketone derived from commercially available aldehyde <NUM> would provide access to both isomers.

The synthesis of callyspongiolide begins from known chiral alcohol <NUM> as shown in Scheme <NUM>, which is prepared in four steps from commercially available (S)-(+)-<NUM>-bromo-<NUM>-methyl-<NUM>-propanol. Treatment of allyl alcohol <NUM> with acryloyl chloride and triethylamine gave diene <NUM>, which was then subjected to ring closing metathesis using Grubbs II catalyst to give α,β-unsaturated lactone <NUM>. <NUM>,<NUM>-addition using Me<NUM>CuLi<NUM>I delivered the methyl substituent stereoselectively to the bottom face of the molecule, providing the corresponding <NUM>,<NUM>-anti-substituted lactone <NUM> in <NUM>% yield as a single diastereomer (determined by <NUM>H NMR). Treatment of the tert-butyl diphenylsilyl (TBDPS) ether with tetrabutyl ammonium fluoride (TBAF) smoothly afforded the corresponding alcohol, which opon oxidation using Dess-Martin periodinane DMP in the presence of NaHCO<NUM> furnished aldehyde <NUM> in <NUM>% yield.

Known diol <NUM> is readily prepared in four steps from commercially available <NUM>-butyne-<NUM>,<NUM>-diol as described by Crimmins et al. Selective Mitsunobu displacement with <NUM>-phenyl-<NUM>H-tetrazole-<NUM>-thiol (PTSH, <NUM>) followed by protection of the latent secondary alcohol provided sulfide <NUM>. See Scheme <NUM>. Oxidation using m-chrloroperbenzoic acid (m-CPBA) afforded sulfone <NUM>, which was now set for the modified Julia olefination reaction. Metallation using lithium hexamethyldisilazide (LHMDS) in dimethyl formamide (DMF) at -<NUM> followed by addition of aldehyde <NUM> gave the desired trans-disubstituted olefin <NUM> in <NUM>% yield with a trans/cis ratio of <NUM>:<NUM> as determined by <NUM>H NMR.

The lactone <NUM> was opened using N,O-dimethylhydroxylamine hydrochloride and iPrMgCl in tetrahydrofuran (THF) at -<NUM> to provide the corresponding Weinreb amide (Scheme <NUM>). The secondary alcohol was protected as the tert-butyl dimethyl silyl (TBS) ether with TBSCI and trimethylamine in either DMF or dichloromethane (DCM) to obtain TBS ether <NUM> in <NUM>% yield over two steps. Reduction using diisobutyl aluminum hydride (DIBAL-H) followed by treatment with the Ohira-Bestmann reagent and K<NUM>CO<NUM> in MeOH gave the homologated alkyne <NUM>. The terminal acetylene moiety was alkylated by metallation with n-BuLi in THF at -<NUM> followed by treatment with ethyl chloroformate to give alkynyl ester <NUM>. Selective removal of the triethyl silyl (TES) ether using pyridinium p-toluenesulfonate (PPTS) in methanol at <NUM> followed by LiOH-mediated ester hydrolysis provided the corresponding seco acid. Yonemitsu's variation for macrolactonization proved highly effective, whereby the mixed anhydride is not preformed but rather the seco acid is directly added to <NUM>,<NUM>,<NUM>-trichlorobenzoyl chloride, <NUM>-dimethylaminopyridine (DMAP), and amine base from the beginning to furnish alkynyl lactone <NUM> in <NUM>% yield over <NUM> steps. Reduction of the alkynyl moiety was accomplished by using Lindlar catalyst poisoned with quinoline under a hydrogen balloon. Removal of the TBS ether was ineffective using tetra-n-butylammonium fluoride (TBAF), even at elevated temperatures. Alternatively, use of HF·pyridine was highly efficient and gave the corresponding alcohol. Chlorosulfonyl isocyanate was used to install the carbamate moiety in <NUM>% yield over both steps. Removal of the para-methoxybenzyl (PMB) protecting group was accomplished using <NUM>,<NUM>-dichloro-<NUM>,<NUM>-dicyanobenzoquinone (DDQ) to provide the corresponding alcohol, which was oxidized using DMP and then subjected to Takai olefination to furnish vinyl iodide <NUM> in <NUM>% yield over three steps.

Commercially available <NUM>-bromo-<NUM>-hydroxybenzaldehyde (<NUM>) was protected as the TBS phenol (Scheme <NUM>) followed by reverse prenylation using <NUM>-methyl-<NUM>-butenylmagnesium chloride to give alcohol <NUM> in <NUM>% yield over <NUM> steps. The racemic alcohol was oxidized using pyridinium chlorochromate (PCC)/SiO<NUM> in DCM to give the corresponding ketone, which was subjected to chiral reduction using Corey's (S)-Me-CBS catalyst to furnish alcohol (S)-<NUM> in <NUM>% yield and <NUM>% ee (determined by chiral HPLC). Synthesis and X-ray crystallography of the <NUM>-nitrobenzoate derivative confirmed the stereochemical outcome. Triethylsilyl triflate (TESOTf) protected the hindered alcohol. Ozonolysis of the terminal olefin gave a mixture of products, including silyl deprotection. Nicolau's conditions for oxidative cleavage of olefins provided the corresponding aldehyde, which was then subjected to Wittig olefination with commercially available phosphonium bromide <NUM> to give the corresponding TMS-protected enyne in a trans/cis ratio of <NUM>:<NUM>. Global deprotection of all silyl protecting groups was achieved using TBAF to afford enyne (S)-<NUM> in <NUM>% yield over <NUM> steps. In the same manner, (R)-<NUM> was prepared using the same chemistry while employing (R)-Me-CBS during the chiral reduction.

Sonogashira coupling of vinyl iodide <NUM> with (S)-<NUM> gave the desired dienyne (S)-<NUM> in <NUM>% yield over <NUM> steps (Scheme <NUM>). Similarly, coupling with the (R)-enantiomer furnished dienyne (R)-<NUM> in <NUM>% yield over <NUM> steps.

The synthetic methods presented herein provide access to analogs of the compound of formula (I) and/or (II), such as compounds <NUM>-<NUM>:
<CHM>
<CHM>
<CHM>
<CHM>
Compounds <NUM> to <NUM> do not fall within the scope of the claims.

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added Et<NUM>N (<NUM>, <NUM> mmol, <NUM> equiv. ) and acryloyl chloride (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> minutes, silica gel was added and the mixture was concentrated via rotary evaporation. The crude product was purified by column chromatography (<NUM>% EA/HX) to give <NUM> (<NUM>% yield) of diene as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> <NUM> (c <NUM>, chloroform).

A deep violet solution of diene (<NUM>, <NUM> mmol) and Grubbs II catalyst (<NUM>, <NUM> mmol, <NUM> mol %) in DCM (<NUM>) was heated to reflux while covered in aluminum foil. The reaction was quenched with ethyl vinyl ether (<NUM>, <NUM> mmol, <NUM> equiv) after <NUM> hrs. and left stirring <NUM> hr. Concentration via rotary evaporation followed by column chromatography purification (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of α,β-unsaturated lactone as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> (c <NUM>, chloroform).

To a tan suspension of Cul (<NUM>, <NUM> mmol, <NUM> equiv. ) in Et<NUM>O (<NUM>) at <NUM> was added MeLi (<NUM> DEM, <NUM>, <NUM> mmol, <NUM> equiv. After stirring <NUM> minutes, the clear solution was cooled to -<NUM> and a clear solution of lactone (<NUM>, <NUM> mmol) in Et<NUM>O (<NUM>) was added slowly via cannula. The flask was allowed to warm to -<NUM> over the course of <NUM> hrs. and then quenched with sat. The crude product was extracted with EA (x3), washed in brine and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of lactone as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (sep, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>).

To a clear solution of TBDPS ether (<NUM>, <NUM> mmol) in THF (<NUM>) was added TBAF (<NUM> THF, <NUM>, <NUM> mmol, <NUM> equiv. After <NUM> minutes, the reaction was quenched with sat. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of alcohol as a clear oil.

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added portionwise NaHCO<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) and DMP (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> minutes, the ice bath was removed and the reaction stirred for a further <NUM> hrs. at room temperature. The reaction was quenched with a <NUM>:<NUM> solution of sat. NaHCO<NUM>/Na<NUM>S<NUM>O<NUM> solution and left stirring vigorously <NUM> minutes. The crude product was then extracted with DCM (x3), washed in brine, and dried over MgSO<NUM>. Purification through a short silica plug (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of aldehyde as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>.

To a clear solution of diol (<NUM>, <NUM> mmol), PPh<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ), and PTSH (<NUM>, <NUM> mmol, <NUM> equiv. ) in THF (<NUM>) at -<NUM> was DIAD (<NUM>, <NUM> mmol, <NUM> equiv. After <NUM> hr. , silica gel was added and the mixture concentrated via rotary evaportation. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of sulfide as a clear oil.

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added Et<NUM>N (<NUM>, <NUM> mmol, <NUM> equiv. ) and TESOTf (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> minutes the reaction was quenched with sat. NaHCO<NUM> and the flask warmed to room temperature. The crude product was extracted with DCM (x3), washed in brine, and dried over MgSO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of sulfide as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of sulfide (<NUM>, <NUM> mmol) in DCM (<NUM>) was added portionwise NaHCO<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) and m-CPBA (<NUM> wt %, <NUM>, <NUM> mmol) sequentially. After <NUM> hrs, the flask was cooled to <NUM> and carefully quenched with sat. NaHSO<NUM>. The crude product was extracted with DCM (x3), washed in sat. NaHCO<NUM>, brine, and dried over MgSO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of sulfone as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>); <NUM>C NMR (<NUM>, CDCI<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>. ; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of sulfone (<NUM>, <NUM> mmol, <NUM> equiv. ) in DMF (<NUM>) at -<NUM> was added LHMDS (<NUM> THF, <NUM>, <NUM> mmol, <NUM> equiv. ) slowly over <NUM> minutes. After stirring <NUM> minutes, a clear solution of aldehyde (<NUM>, <NUM> mmol) in DMF (<NUM>) was added slowly via cannula. After <NUM> hrs, the reaction was quenched with sat. NH<NUM>CI and flask warmed to room temperature. The crude product was diluted with H<NUM>O and extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield, <NUM>:<NUM> trans/cis) of lactone as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a cloudy mixture of lactone (<NUM>, <NUM> mmol) and amine salt (<NUM>, <NUM> mmol, <NUM> equiv. ) in THF (<NUM>) at -<NUM> was added iPrMgCl (<NUM> THF, <NUM>, <NUM> mmol, <NUM> equiv. After <NUM> minutes, the reaction was quenched with sat. NH<NUM>CI and flask warmed to room temperature. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of Weinreb amide as a clear oil.

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DMF (<NUM>) at <NUM> was added Et<NUM>N (<NUM>, <NUM> mmol, <NUM> equiv. ) and TBSCI (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> hours, the reaction was highly diluted with H<NUM>O. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of TBS ether as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>, -<NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of aldehyde (<NUM>, <NUM> mmol) in THF (<NUM>) at -<NUM> was added DIBAL-H (<NUM> HX, <NUM>, <NUM> mmol, <NUM> equiv. After <NUM> minutes the reaction was quenched with a few drops of MeOH, diluted with EA, quenched with potassium sodium tartrate and flask warmed to room temperature. After stirring vigorously <NUM> hrs. , the crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (quantitative) of aldehyde as a clear oil.

To a white mixture of aldehyde (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) in MeOH (<NUM>) was added Ohira-Bestmann reagent (<NUM>, <NUM> mmol, <NUM> equiv. After <NUM> hrs. , the reaction was quenched with H<NUM>O. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification through a short silica plug (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of aldehyde as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of alkyne (<NUM>, <NUM> mmol) in THF (<NUM>) at - <NUM> was added n-BuLi (<NUM> HX, <NUM>, <NUM> mmol, <NUM> equiv) dropwise. After stirring <NUM> minutes, a neat solution of ethyl chloroformate (<NUM>, <NUM> mmol, <NUM> equiv. ) was added dropwise. The reaction was quenched with sat. NH<NUM>Cl after <NUM> hr. and the flask was warmed to room temperature. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of alkyne as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM> Hz, <NUM>), <NUM> (d, J=<NUM> Hz, <NUM>), <NUM> (q, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (m, <NUM>), <NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (S, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of TES ether (<NUM>, <NUM> mmol) in EtOH (<NUM>) at <NUM> was added PPTS (<NUM>, <NUM> mmol, <NUM> equiv. ) in one portion. After <NUM> hrs. , the reaction was quenched with sat. NaHCO<NUM> and flask warmed to room temperature. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>.

To a clear solution of alkynyl ester in THF/H<NUM>O (<NUM>:<NUM>, <NUM>) was added LiOH·H<NUM>O (<NUM>, <NUM> mmol, <NUM> equiv. ) in one portion. After <NUM> hours, the reaction was quenched with <NUM> N HCI and the crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>.

To a cloudy mixture of <NUM>,<NUM>,<NUM>-trichlorobenzoyl chloride (<NUM>, <NUM> mmol, <NUM> equiv. ), DIPEA (<NUM>, <NUM> mmol, <NUM> equiv. ), and DMAP (<NUM>, <NUM> mmol, <NUM> equiv. ) in PhMe (<NUM>) was added a clear solution of seco acid in PhMe (<NUM>) slowly via cannula. After <NUM> hrs. , the reaction was quenched with sat. NaHCO<NUM>. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of alkynyl lactone as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>, -<NUM>; <MAT> <NUM> (c <NUM>, chloroform).

A black mixture of alkynyl lactone (<NUM>, <NUM> mmol), quinoline (<NUM>, <NUM> mmol, <NUM> equiv. ), and Lindlar catalyst (<NUM>) in EA/<NUM>-Hexene (<NUM>:<NUM>, <NUM>) was flushed with Ar (x5), H<NUM> (x5), and left vigorously stirring under an H<NUM> balloon. After <NUM> hrs. , the reaction mixture was passed through celite and thoroughly rinsed with EA. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of a,b-unsaturated lactone as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>) <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dq, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, - <NUM>, -<NUM>; <MAT> <NUM> (c <NUM>, chloroform).

HF·py (<NUM>%, <NUM>) was added dropwise to a clear solution of TBS ether (<NUM>, <NUM> mmol) in MeOH (<NUM>) at <NUM> in a plastic vial. After <NUM> hrs. , the reaction was carefully quenched with sat. NaHCO<NUM> and the vial was warmed to room temperature. The crude product was extracted with EA (x3), washed in sat. NaHCO<NUM>, brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of alcohol as a clear oil.

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added neat chlorosulfonyl isocyanate (<NUM>, <NUM> mmol, <NUM> equiv. ) dropwise. After <NUM> minutes, the reaction was slowly quenched with THF/H2O (<NUM>:<NUM>, <NUM>) and flask warmed to room temperature. Following <NUM> hrs. of vigorous stirring, the reaction was quenched with sat. NaHCO<NUM>. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of carbamate as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dq, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear/yellow biphasic mixture of PMB ether (<NUM>, <NUM> mmol) in DCM/pH <NUM> phosphate buffer (<NUM>:<NUM>, <NUM>) was added DDQ (<NUM>, <NUM> mmol, <NUM> equiv. After <NUM> hr. , additional DDQ was added (<NUM>, <NUM> mmol, <NUM> equiv. The reaction was quenched following <NUM> hrs. and crude product extracted with EA (x3), washed in sat. NaHCO<NUM>, brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of alcohol as a clear oil.

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added NaHCO<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) and DMP (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. The ice bath was removed after addition, reaction left stirring <NUM> hrs. , then quenched with <NUM> sat. Na<NUM>S<NUM>O<NUM>/sat. NaHCO<NUM> (<NUM>:<NUM>). The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification through a short silica plug (<NUM>% EA/HX) gave <NUM> of aldehyde as a white solid.

A yellow solution of aldehyde (<NUM>, <NUM> mmol) and CHI<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) in THF (<NUM>) was added via cannula to a green suspension of CrCl<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) in THF (<NUM>). After <NUM> hrs. , the reaction was quenched with H<NUM>O and crude product extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification through a short silica plug (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of vinyl iodide as a white solid.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>-<NUM> (m, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM> (brs, <NUM>), <NUM> (ddd, J=<NUM>, <NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dq, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>); further spectroscopic data was not collected due to concern of decomposition.

To a grey mixture of phenol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added DIPEA (<NUM>, <NUM> mmol, <NUM> equiv. ) and TBSCI (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> hrs. , the yellow solution was quenched with sat. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of TBS phenol as a clear oil.

To a clear solution of aldehyde (<NUM>, <NUM> mmol) in THF (<NUM>) at <NUM> was added a grey solution of <NUM>-methyl-<NUM>-butenylmagnesium chloride (<NUM> THF, <NUM>, <NUM> mmol, <NUM> equiv. After <NUM> minutes, the reaction was quenched with sat. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of alcohol as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (t, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>, -<NUM>.

To a cloudy suspension of alcohol (<NUM>, <NUM> mmol) and SiO<NUM> (<NUM>) in DCM (<NUM>) was added PCC (<NUM>, <NUM> mmol, <NUM> equiv. ) portionwise. After <NUM> hrs. , the reaction was filtered through celite and the filter cake was washed with DCM. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of ketone as a clear oil.

To a faint-yellow solution of ketone (<NUM>, <NUM> mmol) and (S)-Me-CBS (<NUM>, <NUM> mmol, <NUM> equiv. ) in PhMe (<NUM>) at <NUM> was added BMS (<NUM>, <NUM> mmol, <NUM> equiv. ) dropwise. After <NUM> hrs. , the reaction was quenched with MeOH dropwise, flask warmed to room temperature, and concentrated via rotary evaporation. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of alcohol as a clear oil. <MAT> (c <NUM>, chloroform). Chiral HPLC: Chiralpak IC3 (<NUM> x <NUM>), <NUM>% IPA/HX, flow = <NUM>/min, T = <NUM>, UV = <NUM>, Rt major = <NUM>, Rt minor = <NUM>.

Prepared in the same manner as alcohol (S)-<NUM> above employing (R)-Me-CBS catalyst. <MAT> -<NUM> (c <NUM>, chloroform).

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) was added Et<NUM>N (<NUM>, <NUM> mmol, <NUM> equiv. ), <NUM>-nitrobenzoyl chloride (<NUM>, <NUM> mmol, <NUM> equiv. ), and DMAP (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> hrs. , the reaction was quenched with sat. NaHCO<NUM>. The crude product was extracted with DCM (x3), washed in brine, and dried over MgSO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of benzoate as an opaque solid.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (d, J=<NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of alcohol (<NUM>, <NUM> mmol) in DCM (<NUM>) at <NUM> was added Et<NUM>N (<NUM>, <NUM> mmol, <NUM> equiv. ) and TESOTf (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. After <NUM> minutes, the reaction was quenched with sat. NaHCO<NUM> and flask warmed to room temperature. The crude product was extracted with DCM (x3), washed in brine, and dried over MgSO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of TES ether as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (t, J=<NUM>, <NUM>), <NUM> (quint, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, -<NUM>, -<NUM>; <MAT> -<NUM> (c <NUM>, chloroform).

Prepared in the same manner as TES ether (S)-<NUM> above. <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of olefin (<NUM>, <NUM> mmol) in acetone/H<NUM>O (<NUM>) was added <NUM>,<NUM>-lutidine (<NUM>, <NUM> mmol, <NUM> equiv. ), NMO (<NUM>, <NUM> mmol, <NUM> equiv. ), and OsO<NUM> (<NUM>% in H<NUM>O, <NUM>, <NUM> mmol, <NUM> equiv. ) sequentially. The yellow solution was left stirring overnight while covered with aluminum foil. After <NUM> hrs. , Phl(OAc)<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) was added and reaction left stirring additional <NUM> minutes. The reaction was quenched with Na<NUM>S<NUM>O<NUM> and left vigorously stirring <NUM> minutes. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of aldehyde as a clear oil.

To a cream-colored suspension of phosphonium bromide (<NUM>, <NUM> mmol, <NUM> equiv. ) in THF (<NUM>) at -<NUM> was added LHMDS (<NUM> THF, <NUM>, <NUM> mmol, <NUM> equiv. After <NUM> minutes, the flask was warmed to - <NUM> for an additional <NUM> minutes, then recooled to -<NUM>. A clear solution of aldehyde (<NUM>, <NUM> mmol) in THF (<NUM>) was then added slowly via cannula. The flask was then slowly warmed to room temperature and left stirring overnight. After <NUM> hrs. , the reaction was quenched with sat. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> of TMS-enyne as a clear oil with a <NUM>:<NUM> trans/cis ratio as determined by <NUM>H NMR.

To a clear solution of tri-silyl ether (<NUM>, <NUM> mmol) in THF (<NUM>) was added TBAF (<NUM> THF, <NUM>, <NUM> mmol, <NUM> equiv. After <NUM> hrs. , the reaction was quenched with sat. The crude product was extracted with EA (x3), washed in brine, and dried over Na<NUM>SO<NUM>. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of enyne as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM> (t, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>); <NUM>C NMR (<NUM>, CDCl<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> (c <NUM>, chloroform).

To a clear solution of vinyl iodide (<NUM>, <NUM> mmol) and enyne (<NUM>, <NUM> mmol, <NUM> equiv. ) in degassed PhMe (<NUM>) was added pyrrolidine (<NUM>, <NUM> mmol, <NUM> equiv. ) and Cul (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially to give a faint-red solution. After stirring <NUM> minutes, Pd(PPh<NUM>)<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) was was added to produce a faint-yellow solution. Following <NUM> minutes, the reaction was passed through a short plug of silica and eluted with Et<NUM>O. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield over <NUM>-steps) of callyspongiolide, (S)-<NUM> as a clear oil.

Rf = <NUM> (<NUM>% EA/HX); <NUM>H NMR (<NUM>, DMSO-d<NUM>) d (ppm): <NUM> (brs, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (td, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (t, J=<NUM>, <NUM>), <NUM> (app td, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (dd, J=<NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, J=<NUM>, <NUM>); <NUM>C NMR (<NUM>, DMSO-d<NUM>) d (ppm): <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>; <MAT> <NUM> (c <NUM>, chloroform).

To a clear solution of vinyl iodide (<NUM>, <NUM> mmol) and enyne (<NUM>, <NUM> mmol, <NUM> equiv. ) in degassed PhMe (<NUM>) was added pyrrolidine (<NUM>, <NUM> mmol, <NUM> equiv. ) and Cul (<NUM>, <NUM> mmol, <NUM> equiv. ) sequentially to give a faint-red solution. After stirring <NUM> minutes, Pd(PPh<NUM>)<NUM> (<NUM>, <NUM> mmol, <NUM> equiv. ) was was added to produce a faint-yellow solution. Following <NUM> minutes, the reaction was passed through a short plug of silica and eluted with Et<NUM>O. Purification by flash chromatography (<NUM>% EA/HX) gave <NUM> (<NUM>% yield) of callyspongiolide, (R)-<NUM> as a clear oil.

Claim 1:
A compound of the formula (III):
<CHM>
or a pharmaceutically acceptable salt, polymorph, solvate or clathrate thereof
wherein:
each dashed bond independently represents a double bond in the configuration shown;
R<NUM> is N(R<NUM>)<NUM>, wherein each R<NUM> independently represents H, alkyl, aryl, alkaryl, or arylalkyl;
X<NUM>, X<NUM>, X<NUM> and X<NUM> are each O;
each R<NUM> is, independently, C<NUM>-C<NUM>alkyl;
each R<NUM> is, independently, H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR<NUM>,
S(O)pR<NUM> or N(R<NUM>)<NUM>;
R<NUM> is OR<NUM> or N(R<NUM> )<NUM>; and
each R<NUM> is, independently, H, halo, alkyl, aryl, alkaryl, arylalkyl, heterocyclyl, OR<NUM>, S(O)pR<NUM> or N(R<NUM>)<NUM>; and
wherein the compound of the formula (III) is not a compound of the formula (I) or (II):
<CHM>
<CHM>