Patent Description:
With the improvement of the living standard of people, pesticide residues, viruses, nutritional elements or other aspects of some edible food materials are usually required to be detected in daily life, so as to qualitatively or quantitatively obtain the conditions of the food materials. For example, due to the pesticide abuse problem, fruits, vegetables and agricultural and sideline products purchased daily by people may have the problem of excessive pesticide residue content, and if the problem of excessive pesticide residue content of the foods cannot be found in time, great harm may be caused after people ingest the foods. For another example, currently advocated breast feeding is best feeding for infants only when breast milk has normal nutritional value, but in cases of diseases, medicine taking, surgery or other cases of the mother, the milk secreted by the mother may have reduced content of nutritional elements and even produce viruses, thereby affecting the growth and health of the infants.

Among detection methods, the method for detection by using a microfluidic biochip is rapid, the size is small, and the method is suitable for household use. In order to make sample introduction of the microfluidic biochip more accurate and easy to control, a driving device may be used to drive sample liquid into the microfluidic biochip; at this point, the quality of docking between the driving device and the microfluidic biochip directly affects the effect and accuracy of the driven sample introduction. <CIT> discloses a microfluidic chip device for detecting activated monocytes from a sample of blood. The microfluidic chip device contains a microfluidic chip and a sensor. The microfluidic chip has a flow channel coupled to a sample inlet and an outlet. The sensor has an inner surface coated with anti-CD1 <NUM> c antibodies or VLA-<NUM> receptor substrate, the sensor fluidly coupled to the flow channel, the sensor configured to shear white cells in a volume of blood flowing through the flow channel and the sensor. <CIT> discloses a pesticide residue detecting pen. The detecting pen contains a microfluidic chip, a micro syringe pump, a control system, a semiconductor light source, a photosensitive detector and a casing. The microfluidic chip is detachably connected to the micro syringe pump, and the control System controlling the micro syringe pump, the semiconductor light source, and the photosensitive detector, the micro syringe pump, the control system, the semiconductor light source, and the photosensitive detector are all located in the housing. <CIT> discloses a detachable plankton microfluidic chip for high-power microscopic image acquisition. The detachable plankton microfluidic chip contains a cover sheet, a substrate and a clamp, the clamp comprises an upper clamp body and a lower clamp body, and the upper clamp body is provided with an upper light transmission hole The lower clip body is provided with a lower light transmission hole, and more than one detection channel is arranged on the substrate. <CIT> discloses a modular sample single channel detection chip assembly suitable for a handheld SPR detector. The modular sample single channel detection chip assembly contains a liquid sample collection and storage device, a sensor chip, and a handheld SPR detector housing connection device. The liquid sample collection storage device contains a liquid sample collection device and a liquid sample storage device. <CIT> discloses a device for analyzing a liquid sample possibly mixed with one or more reagents. The device includes (a) a system for taking a liquid sample which can be moved in one direction, vertical and a horizontal direction with a view to dipping, taking and bringing said sample into a measuring cuvette, and subjecting it to an analytical reaction, and (b) a photometric analyzer capable of carrying out an analytical determination of the sample. <CIT> discloses a smart home capable of detecting food safety. The smart home includes a home, a food safety detector and a microfluidic chip. The food safety detector is embedded in the household, the microfluidic chip and the food safety detection a detachable connection.

An object of a first aspect of the present invention is to overcome at least one of the drawbacks of the prior art, and to provide a microfluidic detection system suitable for a refrigerator, which has a good sample introduction effect and realizes accurate sample introduction control.

A further object of the first aspect of the present invention is to prolong the service life of the microfluidic detection system.

Another object of the first aspect of the present invention is to improve the reliability and sealing effect of sealed docking between a microfluidic biochip and a sample liquid driving device.

An object of a second aspect of the present invention is to provide a refrigerator having the above microfluidic detection system.

According to a first aspect of the present invention, there is provided a microfluidic detection system for a refrigerator, including:.

According to the invention, an end surface of an extended tip of the plug pin is a continuous and smooth hemispherical surface, and a pin hole of the plug pin for fluid communication with the sealed docking mechanism is formed on the circumferential side of a section of the plug pin located inside the sealed docking mechanism.

Optionally, a sealing ring for abutting contact or pressing contact with the plug pin is formed on the sealed docking mechanism, a through hole for the plug pin to pass through is formed in the middle of the sealing ring, and a sealing reinforcing rib protruding towards the radial inner side of the through hole is formed on the circumferential wall of the through hole.

Optionally, the outer wall of an end section of the plug pin for insertion into the sealed docking mechanism is tapered in an insertion direction of the plug pin; and
the pin hole of the plug pin for fluid communication with the sealed docking mechanism is formed on the end surface of the extended tip of the plug pin.

Optionally, the plug pin is inserted into the microfluidic biochip through the communication port, and a starting end of the plug pin extending into the microfluidic biochip is open; and
a matching interface between the plug pin and the communication port is sealed by sealing gum.

Optionally, the sealed docking mechanism is connected between the microfluidic biochip and the sample liquid driving device, a through channel penetrating through the sealed docking mechanism is formed in the sealed docking mechanism, and the sample liquid driving device and the communication port are both communicated with the through channel;
the sample liquid driving device is provided with a connecting pipeline connected with the sealed docking mechanism, the sealed docking mechanism includes a first connecting piece and a second connecting piece which are sequentially arranged between the sample liquid driving device and the microfluidic biochip, a first connecting column and a second connecting column protruding and extending in opposite directions are formed on two opposite sides of the first connecting piece respectively, the first connecting column is inserted into the connecting pipeline, the second connecting column is inserted into the second connecting piece, and the plug pin is in insertion-connection with the second connecting piece.

Optionally, the microfluidic detection system further includes:.

Optionally, the elastic pressing mechanism is a spring, one end of the spring abuts against a fixedly arranged end plate, the other end of the spring abuts against the sealed docking mechanism, and the end plate and the microfluidic biochip are located on two opposite sides of the sealed docking mechanism respectively, such that the spring generates the elastic acting force towards the microfluidic biochip on the sealed docking mechanism.

Optionally, the chip mounting mechanism includes two oppositely arranged elastic clamping jaws, so as to apply opposite acting forces to the microfluidic biochip clamped between the two elastic clamping jaws; and
the microfluidic detection system further includes a cantilever button which is suspended on one side of the microfluidic biochip, the cantilever button abuts against the inner sides of the two elastic clamping jaws at the same time, and acting forces which promote the elastic clamping jaws to elastically deform in directions of departing from each other are applied to the two elastic clamping jaws when the cantilever button is subjected to acting forces towards the elastic clamping jaws, so as to relieve the clamping effect of the two elastic clamping jaws on the microfluidic biochip.

According to a second aspect of the present invention, there is further provided a refrigerator including the microfluidic detection system according to any one of the above-mentioned solutions.

The microfluidic detection system according to the present invention includes the sealed docking mechanism, the sealed docking mechanism and the sample liquid driving device form the fluid-tight connection, and particularly, the plug pin protruding and extending outward is fixedly connected to the communication port of the microfluidic biochip in the present invention, the internal flow channel of the plug pin is in sealed communication with the communication port, and the plug pin is inserted into the inside of the sealed docking mechanism and forms the fluid-tight connection with the sealed docking mechanism. That is, the sealed docking mechanism is simultaneously in fluid-tight connection with the sample liquid driving device and the plug pin, thus realizing a sealed communication relationship between the sample liquid driving device and the communication port of the microfluidic biochip, avoiding that the problems of air leakage, liquid leakage, or the like, are generated to influence the pressure in a main channel formed by sequential communication of the sample inlet, the detection pool and the communication port, so as to influence entrance of the sample liquid into the main channel, guaranteeing a better sample introduction effect of the microfluidic detection system, and facilitating accurate control over a sample introduction process using the sample liquid driving device.

Further, in the present application, the pin hole is formed in the circumferential side of the section of the plug pin located inside the sealed docking mechanism, the end surface of the extended tip of the plug pin is designed to be the continuous and smooth hemispherical surface, and when the microfluidic biochip is hermetically docked with the sealed docking mechanism, the contact surface between the plug pin and the sealed docking mechanism is a smooth spherical surface, such that the sealed docking mechanism cannot be scratched or punctured, the sealed docking mechanism is guaranteed to keep a good sealed docking function for a long time, and the service life of the microfluidic detection system is prolonged.

Further, the microfluidic detection system according to the present application further includes the elastic pressing mechanism, and the elastic acting force towards the microfluidic biochip can be applied to the sealed docking mechanism by the elastic pressing mechanism, such that the sealed docking mechanism is promoted to be always kept in a state of being tightly and hermetically docked with the sample liquid driving device and the microfluidic biochip at the same time, and the problems of looseness, breakage, or the like, caused by long-time use of other docking mechanisms are avoided, thereby guaranteeing a long-term and reliable fluid-tight communication relationship between the sample liquid driving device and the communication port of the microfluidic biochip, and improving the sealing effect therebetween.

According to the following detailed description of specific embodiments of the present invention in conjunction with drawings, those skilled in the art will better understand the aforementioned and other objects, advantages and features of the present invention.

Some specific embodiments of the present invention will be described below in detail in an exemplary rather than restrictive manner with reference to the drawings. Identical reference numerals in the drawings represent identical or similar components or parts. Those skilled in the art should understand that these drawings are not necessarily drawn to scale. In the drawings:.

The present invention firstly provides a microfluidic detection system for a refrigerator, which is used for qualitatively or quantitatively detecting a preset detection parameter of sample liquid; the preset detection parameter may be, for example, a pesticide residue parameter for indicating whether a pesticide residue content exceeds the standard and/or a specific value of the pesticide residue content, a nutrient parameter for indicating whether a nutritional element meets the standard and/or a specific content of the nutritional element, a specific substance parameter for indicating whether a specific harmful substance (for example, a specific virus) exceeds the standard and/or a specific content thereof, or the like.

<FIG> is a schematic structural diagram of the microfluidic detection system for a refrigerator according to one embodiment of the present invention, <FIG> is a schematic exploded structural diagram of the microfluidic detection system for a refrigerator according to one embodiment of the present invention, <FIG> is a schematic structural diagram of an internal structure of the microfluidic detection system according to one embodiment of the present invention, and <FIG> is a schematic exploded structural diagram of the internal structure of the microfluidic detection system according to one embodiment of the present invention. For ease of understanding, a sample cup <NUM> is also shown in <FIG>.

Referring to <FIG>, the microfluidic detection system <NUM> according to the present invention includes a microfluidic biochip <NUM>, a sample liquid driving device <NUM> and a detection mechanism <NUM>. It may be appreciated by those skilled in the art that specific selection of the microfluidic biochip <NUM> and the detection mechanism <NUM> used in the microfluidic detection system may vary when the preset detection parameters detected by the microfluidic detection system vary. For example, when the microfluidic detection system is used for pesticide residue detection, the microfluidic biochip <NUM> thereof can be a microfluidic pesticide residue detection chip capable of providing detection conditions for pesticide residue liquid, and the detection mechanism <NUM> thereof can be a pesticide residue detection mechanism capable of detecting pesticide residue parameters of the pesticide residue liquid.

<FIG> is a schematic structural diagram of the microfluidic biochip in one embodiment of the present invention; referring to <FIG>, the microfluidic biochip <NUM> has a sample inlet <NUM>, a communication port <NUM> and a detection pool <NUM> formed therein, and the sample inlet <NUM>, the detection pool <NUM> and the communication port <NUM> communicate in sequence by means of a microfluidic channel <NUM> to form a main channel. The microfluidic channel <NUM> in the present invention means a micro flow channel or a capillary flow channel having a flow area within a preset size range, so as to have a suitable capability of holding liquid therein. The sample inlet <NUM> and the communication port <NUM> may be formed at an end portion of the microfluidic biochip <NUM>. Further, the sample inlet <NUM> and the communication port <NUM> are preferably formed at different end portions of the microfluidic biochip <NUM>.

The sample liquid driving device <NUM> is in sealed communication with the communication port <NUM> by means of a sealed docking mechanism <NUM>, and used to impel sample liquid in contact with the sample inlet <NUM> to enter the microfluidic channel and flow to the detection pool <NUM> by means of the microfluidic channel. Specifically, the sample liquid driving device <NUM> may form a negative pressure in the main channel by pumping air outwards, such that the sample liquid in contact with the sample inlet <NUM> is allowed to flow into the microfluidic channel <NUM> under the action of the negative pressure.

The detection mechanism <NUM> is used for detecting the detection pool <NUM>, so as to obtain the preset detection parameter of the sample liquid. Specifically, the detection pool <NUM> may be provided therein with a detection reagent in advance, or the detection reagent may be manually or automatically added to the detection pool <NUM>, such that the detection mechanism <NUM> detects the detection pool <NUM> after the sample liquid in the detection pool <NUM> reacts with the detection reagent therein.

In a specific embodiment, when the detection mechanism <NUM> is a pesticide residue detection mechanism for detecting the pesticide residue parameters of the pesticide residue liquid, an enzyme inhibition rate method can be used to rapidly and qualitatively detect whether pesticide residues in the sample liquid exceed the standard. At this point, the microfluidic biochip <NUM> further includes a reaction pool <NUM> formed therein, and the reaction pool <NUM> is located on the main channel formed by sequentially communicating the sample inlet <NUM>, the detection pool <NUM>, and the communication port <NUM>, and is communicated between the sample inlet <NUM> and the detection pool <NUM>, such that the sample liquid firstly reacts with a reaction reagent in the reaction pool <NUM> and then flows into the detection pool <NUM>. The reaction pool <NUM> is communicated with the sample inlet <NUM> through the microfluidic channel <NUM>, and the reaction pool <NUM> is communicated with the detection pool <NUM> through the microfluidic channel <NUM>. The reaction reagent and the detection reagent for pesticide residue detection may be an enzyme reagent and a color developing agent respectively. The reaction pool <NUM> is configured to allow the sample liquid to react with the enzyme reagent therein, and the sample liquid after the reaction with the enzyme reagent flows into the detection pool <NUM> to react with the color developing agent in the detection pool <NUM>. The detection mechanism <NUM> may be selected as a photoelectric detection mechanism and may include a light source <NUM> and a photosensitive element <NUM> arranged on two opposite sides of the microfluidic biochip <NUM> respectively and directly facing the detection pool <NUM>, light emitted from the light source <NUM> is irradiated to the detection pool <NUM>, and light transmitted through the detection pool <NUM> is introduced into the photosensitive element <NUM>, which facilitates judgment of the change in an absorbance in the detection pool <NUM> using a light intensity signal received by the photosensitive element <NUM>, and then facilitates calculation of a pesticide residue inhibition rate. Further, the detection mechanism <NUM> further includes a heating sheet <NUM> for supplying heat to the detection pool <NUM> and a temperature controller <NUM> for controlling the heating power of the heating sheet <NUM> to be constant, such that the sample liquid and the detection reagent in the detection pool <NUM> can react sufficiently and rapidly.

<FIG> is a schematic exploded structural diagram of the sample liquid driving device, the microfluidic biochip and related structures in one embodiment of the present invention, <FIG> is a schematic sectional structural diagram of the sample liquid driving device, the microfluidic biochip and related structures in one embodiment of the present invention, <FIG> is a schematic enlarged diagram of part B in <FIG>, <FIG> is a schematic exploded sectional diagram of the microfluidic biochip and the sealed docking mechanism in one embodiment of the present invention, and <FIG> is a schematic enlarged diagram of part A in <FIG>. Particularly, the communication port <NUM> of the microfluidic biochip <NUM> is fixedly provided with a plug pin <NUM> protruding and extending outward, an internal flow channel <NUM> of the plug pin <NUM> is in sealed communication with the communication port <NUM>, and the plug pin <NUM> is inserted into the inside of the sealed docking mechanism <NUM> and forms a fluid-tight connection with the sealed docking mechanism <NUM>, and the sealed docking mechanism <NUM> is in fluid-tight connection with the sample liquid driving device <NUM>, so that the sample liquid driving device <NUM> is in sealed communication with the communication port <NUM>.

The applicant recognizes that, since the microfluidic biochip <NUM> is generally disposable, the microfluidic biochip <NUM> is required to be replaced frequently, and if the microfluidic biochip <NUM> is directly in fluid-tight connection with the sample liquid driving device <NUM>, certain damage may be caused to the structure of the sample liquid driving device <NUM> (for example, a connecting pipeline <NUM> thereof for being connected with the microfluidic biochip <NUM> having low structural strength and poor abrasion resistance) after a long time, thereby affecting the service life thereof. For this reason, in the present application, the sealed docking mechanism <NUM> is particularly designed between the microfluidic biochip <NUM> and the sample liquid driving device <NUM>. The sealed docking mechanism <NUM> and the sample liquid driving device <NUM> form the fluid-tight connection, and particularly, the plug pin <NUM> protruding and extending outward is fixedly connected to the communication port <NUM> of the microfluidic biochip <NUM> in the present invention, the internal flow channel <NUM> of the plug pin <NUM> is in sealed communication with the communication port <NUM>, and the plug pin <NUM> is inserted into the inside of the sealed docking mechanism <NUM> and forms the fluid-tight connection with the sealed docking mechanism <NUM>. That is, the sealed docking mechanism <NUM> is simultaneously in fluid-tight connection with the sample liquid driving device <NUM> and the plug pin <NUM>, thus realizing a sealed communication relationship between the sample liquid driving device <NUM> and the communication port <NUM> of the microfluidic biochip <NUM>, avoiding that the problems of air leakage, liquid leakage, or the like, are generated to influence the pressure in the main channel formed by sequential communication of the sample inlet <NUM>, the detection pool <NUM> and the communication port <NUM>, so as to influence entrance of the sample liquid into the main channel, guaranteeing a better sample introduction effect of the microfluidic detection system <NUM>, and facilitating accurate control over a sample introduction process using the sample liquid driving device <NUM>.

Specifically, the plug pin <NUM> may be provided with a pin hole <NUM> for fluidly connecting the internal flow channel <NUM> thereof with the inside of the sealed docking mechanism <NUM>, and the pin hole <NUM> is formed in a section of the plug pin <NUM> located inside the sealed docking mechanism <NUM>; that is, the pin hole of the plug pin <NUM> is located inside the sealed docking mechanism <NUM>, so as to guarantee a smooth and good fluid communication relationship therebetween, improve the sealing performance therebetween to a great extent, and avoid the problems of air leakage, liquid leakage, or the like, at the connection therebetween.

In some embodiments, the sealed docking mechanism <NUM> is provided with a sealing ring for abutting contact or pressing contact with the plug pin <NUM>, and a through hole <NUM> for the plug pin <NUM> to pass through is formed in the middle of the sealing ring. The outer diameter of the plug pin <NUM> may be uniform, the plug pin <NUM> has the outer diameter slightly less than the diameter of the through hole <NUM> to be in pressing contact with the sealing ring, or the plug pin <NUM> has the outer diameter equal to the diameter of the through hole <NUM> to be in abutting contact with the sealing ring, thereby realizing good sealing between the sealed docking mechanism <NUM> and the plug pin <NUM>.

Further, a sealing reinforcing rib <NUM> protruding towards the radial inner side of the through hole <NUM> may be formed on the circumferential wall of the through hole <NUM>, so as to improve the sealing performance between the sealing ring and the plug pin <NUM>. Specifically, the cross section of the sealing reinforcing rib <NUM> may have an arc-shaped convex shape, a saw-tooth shape, or another suitable shape.

<FIG> is a schematic exploded sectional diagram of the microfluidic biochip and the sealed docking mechanism in another embodiment of the present invention. In some other embodiments, the outer diameter of the plug pin <NUM> may alternatively be uneven, and the outer circumferential wall of an end section of the plug pin <NUM> for insertion into the sealed docking mechanism <NUM> is tapered in an insertion direction of the plug pin <NUM> (i.e., the direction of outward extension of the plug pin <NUM> from the microfluidic biochip <NUM>), so as to form a reliable sealed connection relationship between the plug pin <NUM> and the sealed docking mechanism <NUM> (specifically, the sealing ring of the sealed docking mechanism <NUM>) by using the shape of the outer circumferential wall of the plug pin <NUM>. In these embodiments, in order to reduce friction between the outer circumferential wall of the plug pin <NUM> and the sealed docking mechanism <NUM>, the pin hole <NUM> of the plug pin <NUM> for fluid communication with the sealed docking mechanism <NUM> may be formed at an end surface of an extended tip of the plug pin <NUM>.

In order to avoid damage to the sealed docking mechanism <NUM> after frequent insertion and removal of the plug pin <NUM>, the structural strength of the sealed docking mechanism <NUM> may be increased, which, however, has a higher requirement for a material of the sealed docking mechanism <NUM>, and even if a material having higher mechanical strength is adopted, the sealed docking mechanism <NUM> may still be structurally damaged after the plug pin <NUM> is inserted and removed a limited number of times. For this reason, the applicant of the present application improves the structure of the plug pin <NUM> from another perspective. Referring to <FIG>, the end surface <NUM> of the extended tip of the plug pin <NUM> is a continuous and smooth hemispherical surface, and the pin hole <NUM> of the plug pin <NUM> for fluid communication with the sealed docking mechanism <NUM> is formed on the circumferential side <NUM> of a section of the plug pin <NUM> located inside the sealed docking mechanism <NUM>. Thus, when the microfluidic biochip <NUM> provided with the plug pin <NUM> is hermetically docked with the sealed docking mechanism <NUM>, the contact surface between the plug pin <NUM> and the sealed docking mechanism <NUM> is a smooth spherical surface, such that friction between the plug pin <NUM> and the sealed docking mechanism <NUM> is reduced, the sealed docking mechanism <NUM> cannot be scratched or punctured, the sealed docking mechanism <NUM> is guaranteed to keep a good sealed docking function for a long time, the service life of the microfluidic detection system <NUM> is prolonged, and meanwhile, the requirement for the structural strength of the sealed docking mechanism <NUM> is reduced.

It should be noted that the extended tip of the plug pin <NUM> means an end of the plug pin <NUM> extending into the sealed docking mechanism <NUM>. Further, the pin hole <NUM> may be formed at the circumferential side <NUM> of a section of the plug pin <NUM> close to the extended tip thereof, and thus, the fluid communication relationship between the plug pin <NUM> and the sealed docking mechanism <NUM> may be guaranteed even if the section of the plug pin <NUM> inserted into the sealed docking mechanism <NUM> is not long.

In some embodiments, the plug pin <NUM> is inserted into the microfluidic biochip <NUM> through the communication port <NUM>, and a starting end of the plug pin <NUM> extending into the microfluidic biochip <NUM> is open to be communicated with the microfluidic channel <NUM>, so as to be communicated with the communication port <NUM>. The matching interface between the plug pin <NUM> and the communication port <NUM> can be sealed by sealing gum <NUM> to enhance the sealing performance between the plug pin <NUM> and the microfluidic biochip <NUM>.

In some alternative embodiments, the plug pin <NUM> and the microfluidic biochip <NUM> may also be integrally formed.

Further, the part of the sealing gum <NUM> exposed outside the communication port <NUM> may be tapered along the insertion direction of the microfluidic biochip <NUM> (i.e., the direction of outward extension of the plug pin <NUM> from the microfluidic biochip <NUM>). A concave hole <NUM> with a shape matched with the shape of the tapered part of the sealing gum <NUM> is formed on the sealed docking mechanism <NUM>, such that after the plug pin <NUM> is inserted into the sealed docking mechanism <NUM>, the concave hole <NUM> and the sealing gum <NUM> form auxiliary sealing, thus further improving the sealing performance between the sealed docking mechanism <NUM> and the plug pin <NUM>.

In some embodiments, the sealed docking mechanism <NUM> is connected between the microfluidic biochip <NUM> and the sample liquid driving device <NUM>, a through channel <NUM> penetrating through the sealed docking mechanism <NUM> is formed in the sealed docking mechanism <NUM>, and the sample liquid driving device <NUM> and the communication port <NUM> are both communicated with the through channel <NUM>.

Specifically, the sample liquid driving device <NUM> may have a connecting pipeline <NUM> connected with the sealed docking mechanism <NUM>. The communication port <NUM> may be formed on the top of the microfluidic biochip <NUM>, and the sample liquid driving device <NUM> may be adjacently provided on the transverse side of the microfluidic biochip <NUM>, so as to prevent the sample liquid driving device <NUM> from being adversely affected by liquid leakage which may be generated by the microfluidic biochip <NUM>. The connecting pipeline <NUM> may be communicated with the top of the sample liquid driving device <NUM> to be bridged between the sample liquid driving device <NUM> and the microfluidic biochip <NUM>.

Further, the sealed docking mechanism <NUM> includes a first connecting piece <NUM> and a second connecting piece <NUM> sequentially arranged between the sample liquid driving device <NUM> and the microfluidic biochip <NUM>, a first connecting column <NUM> and a second connecting column <NUM> protruding and extending in opposite directions are formed on two opposite sides of the first connecting piece <NUM> respectively, and the first connecting column <NUM> is inserted into the connecting pipeline <NUM>, so as to realize the fluid-tight connection between the sample liquid driving device <NUM> and the sealed docking mechanism <NUM>; the second connecting column <NUM> is inserted into the second connecting piece <NUM>, and the plug pin <NUM> is in insertion-connection with the second connecting piece <NUM>, so as to realize the fluid-tight connection between the plug pin <NUM> and the sealed docking mechanism <NUM>. The first connecting column <NUM> and the connecting pipeline <NUM> may be in insertion-connection by means of abutting contact or pressing contact, and the second connecting column <NUM> and the second connecting piece <NUM> may be in insertion-connection by means of abutting contact or pressing contact, so as to improve the sealing performance. The sealing ring may be formed on the second connecting piece <NUM>. When the microfluidic biochip <NUM> is mounted upwards in the vertical direction, the first connecting column <NUM> extends upwards vertically, the second connecting column <NUM> extends downwards vertically, the second connecting piece <NUM> is located below the first connecting piece <NUM>, and the sealing ring is formed at the bottom of the second connecting piece <NUM>.

In some embodiments, the microfluidic detection system <NUM> further includes a chip mounting mechanism <NUM> for fixing the microfluidic biochip <NUM> after the plug pin <NUM> and the sealed docking mechanism <NUM> form the fluid-tight connection, such that the microfluidic biochip <NUM> keeps a state of fluid-tight connection with the sample liquid driving device <NUM>. It should be noted that "fixed" herein means that the microfluidic biochip <NUM> is immovable rather than undetachable after mounted on the chip mounting mechanism <NUM>. Preferably, to facilitate replacement of the microfluidic biochip <NUM>, the microfluidic biochip <NUM> is detachably mounted on the chip mounting mechanism <NUM>.

Further, the microfluidic detection system <NUM> further includes an elastic pressing mechanism <NUM> for applying an elastic acting force to the sealed docking mechanism <NUM>, such that the sealed docking mechanism <NUM> is elastically and hermetically docked with the sample liquid driving device <NUM> and the microfluidic biochip <NUM> at the same time. Specifically, the elastic acting force towards the microfluidic biochip <NUM> can be applied to the sealed docking mechanism <NUM> by the elastic pressing mechanism <NUM>, and the force of reaction of the microfluidic biochip <NUM> to the sealed docking mechanism <NUM> is used for promoting the sealed docking mechanism <NUM> to be elastically and hermetically docked with the sample liquid driving device <NUM>, such that the sealed docking mechanism <NUM> is always kept in a state of being tightly and hermetically docked with the sample liquid driving device <NUM> and the microfluidic biochip <NUM> at the same time, and the problems of looseness, breakage, or the like, caused by long-time use of other docking mechanisms are avoided, thereby guaranteeing a long-term and reliable fluid-tight communication relationship between the sample liquid driving device <NUM> and the communication port <NUM> of the microfluidic biochip <NUM>, and improving the sealing effect therebetween.

In some embodiments, the elastic pressing mechanism <NUM> may be a spring, one end of the spring abuts against a fixedly arranged end plate <NUM>, the other end of the spring abuts against the sealed docking mechanism <NUM>, and the end plate <NUM> and the microfluidic biochip <NUM> are located on two opposite sides of the sealed docking mechanism <NUM> respectively, such that the spring generates the elastic acting force towards the microfluidic biochip <NUM> on the sealed docking mechanism <NUM>. Specifically, in a mounted state of the microfluidic biochip <NUM>, the spring is in a compressed state to generate the elastic acting force for urging the sealed docking mechanism <NUM> to have a tendency to move towards the microfluidic biochip <NUM>. The number of the elastic pressing mechanisms <NUM> may be two or more, so as to increase the elastic acting force acting on the microfluidic biochip <NUM>, and to make the elastic acting force applied to the microfluidic biochip <NUM> more balanced, thereby avoiding inclination and further improving the sealed connection effect. Specifically, the end plate <NUM> may be formed on the chip mounting mechanism <NUM>, the chip mounting mechanism <NUM> is fixed on a bracket <NUM>, the bracket <NUM> is fixed on a support plate <NUM>, and the support plate <NUM> is fixedly provided in a housing <NUM>.

In some embodiments, the microfluidic detection system <NUM> further includes a guide rod <NUM> sleeved with the spring to prevent the spring from being displaced. One end of the guide rod <NUM> is fixedly connected with the sealed docking mechanism <NUM>, and the other end of the guide rod is in contact with a Hall switch <NUM> after the plug pin <NUM> and the sealed docking mechanism <NUM> form the fluid-tight connection, such that the Hall switch <NUM> is prompted to generate a trigger signal for indicating that the microfluidic biochip <NUM> is mounted in place, so as to prompt a user, thus avoiding structural damage caused by excessive mounting of the microfluidic biochip <NUM>, and meanwhile improving the use experience of the user.

Specifically, the guide rod <NUM> may pass through the end plate <NUM> and be limited and supported by the end plate <NUM>. When the microfluidic biochip <NUM> is mounted upwards in the vertical direction, the head of the guide rod <NUM> above the end plate <NUM> may be provided with an expanded portion, and when the microfluidic biochip <NUM> is not mounted, the spring is in a natural state, and the guide rod <NUM> and the sealed docking mechanism <NUM> move downwards under the action of the gravity thereof until the expanded portion of the guide rod <NUM> abuts against the end plate <NUM>, thereby supporting the guide rod <NUM> and the sealed docking mechanism <NUM> to prevent further downward movement thereof.

In some embodiments, the chip mounting mechanism <NUM> includes two oppositely arranged elastic clamping jaws <NUM>, so as to apply opposite acting forces to the microfluidic biochip <NUM> clamped between the two elastic clamping jaws <NUM>.

Further, the microfluidic detection system <NUM> further includes a cantilever button <NUM> suspended on one side of the microfluidic biochip <NUM>, and the cantilever button <NUM> abuts against the oppositely arranged inner sides of the two elastic clamping jaws <NUM> at the same time, so as to apply outward acting forces to the inner sides of the two elastic clamping jaws <NUM> when the cantilever button <NUM> is subjected to an acting force towards the microfluidic biochip <NUM>, such that the two elastic clamping jaws <NUM> elastically deform towards outer side directions departing from each other. That is, when the microfluidic biochip <NUM> is required to be disassembled, a user only needs to press the cantilever button to release the clamping effect of the two elastic clamping jaws <NUM> on the microfluidic biochip <NUM>, so as to release the microfluidic biochip <NUM>, and the operation is quite simple and convenient; the cantilever button <NUM> has a quite simple structure and a quite ingenious design.

After the clamping effect of the two elastic clamping jaws <NUM> on the microfluidic biochip <NUM> is released, under the action of an elastic deformation restoring force of the elastic pressing mechanism <NUM>, an acting force in a direction opposite to the mounting direction of the microfluidic biochip <NUM> is applied to the sealed docking mechanism <NUM>, the sealed docking mechanism <NUM> drives the microfluidic biochip <NUM> to move in the direction opposite to the mounting direction of the microfluidic biochip <NUM>, and the microfluidic biochip <NUM> can be ejected by a predetermined distance under the action of inertia, such that the user can take out the microfluidic biochip conveniently.

In some embodiments, the sample liquid driving device <NUM> may be a micro injection pump, and may form the negative pressure in the main channel by pumping air outwards, such that the sample liquid in contact with the sample inlet <NUM> enters the main channel under the action of the negative pressure. Specifically, the sample liquid driving device <NUM> includes a driving motor <NUM>, a vertically extending injector <NUM>, a lead screw <NUM>, a slider <NUM>, and a piston <NUM>.

The injector <NUM> is fixed on the bracket <NUM>, and the top of the injector <NUM> is in sealed communication with the communication port <NUM> on the top of the microfluidic biochip <NUM> through the connecting pipeline <NUM>. The lead screw <NUM> extends vertically and is connected with the driving motor <NUM> to be rotated under the driving of the driving motor <NUM>. The lead screw <NUM> penetrates through the slider <NUM>, and the slider is in threaded connection with the lead screw <NUM> to move up and down along the lead screw <NUM> with the rotation of the lead screw <NUM>. Specifically, the bracket <NUM> may be provided with a vertically extending guide groove, and the slider <NUM> is located in the guide groove; thus, the movement of the slider <NUM> in the up-down direction is guided through the guide groove. The piston <NUM> is provided inside the injector <NUM> and fixedly connected with the slider <NUM>, so as to be driven by the slider <NUM> to move in the up-down direction, such that the negative pressure is generated in the main channel when the piston moves downwards, and then, the sample liquid in contact with the sample inlet <NUM> is impelled to flow into the microfluidic channel and flows into the detection pool <NUM> through the microfluidic channel, and the sample liquid in the main channel is impelled to flow to the sample inlet <NUM> when the piston moves upwards.

In some embodiments, the microfluidic detection system <NUM> further includes a sample stage <NUM>, the sample stage <NUM> is used for placing the sample cup <NUM>, and the sample cup <NUM> is used for containing the sample liquid. The sample stage <NUM> is configured to be controllably or operatively moved to transport the sample cup <NUM> placed thereon by the sample stage <NUM> to a position allowing the sample liquid in the sample cup <NUM> to be in contact with the sample inlet <NUM> of the microfluidic biochip <NUM>. Thus, sample loading of the microfluidic biochip <NUM> is realized. The user is only required to place the sample cup <NUM> on the sample stage <NUM>, or after placing the sample cup <NUM> on the sample stage <NUM>, the user moves the sample stage <NUM> to a position where the sample liquid is in contact with the sample inlet <NUM> of the microfluidic biochip <NUM>, such that the sample loading operation is quite convenient, and time and labor are saved. In addition, in the present application, the sample stage <NUM> is configured to be movable, thus omitting complex structures, such as a sample liquid delivery pump, a delivery pipeline, a sampling needle, or the like, such that the microfluidic detection system <NUM> has a quite simple structure, and thus is suitable for being integrated on a refrigerator to facilitate family use. Specifically, the sample stage <NUM> is preferably provided below the microfluidic biochip <NUM>, and the sample inlet <NUM> is preferably provided at the lower end of the microfluidic biochip <NUM>, such that the sample inlet <NUM> is conveniently in contact with the sample liquid in the sample cup <NUM> placed on the sample stage <NUM>.

Further, the microfluidic detection system <NUM> further includes a lifting mechanism <NUM> for driving the sample stage <NUM> to move up and down, such that the sample stage <NUM> is switched between a detection position allowing the sample liquid in the sample cup <NUM> placed on the sample stage <NUM> to be in contact with the sample inlet <NUM> and an initial position at a preset distance below the detection position. That is, the sample stage <NUM> may be automatically lifted and lowered by the lifting mechanism <NUM>.

<FIG> is a schematic structural diagram of the lifting mechanism and the sample stage in a disassembled state in one embodiment of the present invention. In some embodiments, the lifting mechanism <NUM> may include a lifting motor <NUM>, a transmission lead screw <NUM>, and a nut <NUM>. The lifting motor <NUM> is used to output a driving force. The transmission lead screw <NUM> is vertically provided and connected with an output shaft of the lifting motor <NUM> to be rotated under the driving of the lifting motor <NUM>. The transmission lead screw <NUM> penetrates through the nut <NUM>, and the nut is in threaded connection with the transmission lead screw <NUM> to move up and down along the transmission lead screw <NUM> with the rotation of the transmission lead screw <NUM>. The sample stage <NUM> is fixedly connected with the nut <NUM>, such that the nut <NUM> drives the sample stage <NUM> to move up and down.

Further, the lifting mechanism <NUM> further includes a slide rail <NUM> and a slider <NUM>. The slide rail <NUM> is provided beside the transmission lead screw <NUM> in parallel with the transmission lead screw <NUM>, the slider <NUM> is movably provided on the slide rail <NUM>, and the sample stage <NUM> is fixedly connected with the slider <NUM>, so that the sample stage <NUM> is guided to move up and down through the cooperation of the slide rail <NUM> and the slider <NUM>. Specifically, the slider <NUM> is driven to move synchronously when the sample stage <NUM> moves in the up-down direction under the action of a driving module, the slider <NUM> is limited on the slide rail <NUM>, and the slide rail <NUM> has guiding and limiting effects on the movement of the slider <NUM>, such that the sample stage <NUM> is indirectly guided and limited, the sample stage <NUM> is prevented from being shifted or jammed in a moving process, and the movement stability of the sample stage <NUM> is improved. Specifically, the sample stage <NUM> may include a horizontal connecting plate <NUM> through which the transmission lead screw <NUM> penetrates and which is fixedly connected with the nut <NUM>, and a vertical connecting plate <NUM> extending upwards perpendicular to the horizontal connecting plate <NUM>, the vertical connecting plate <NUM> being fixedly connected with the slider <NUM>.

In some embodiments, the lifting mechanism <NUM> further includes a limit switch <NUM>, and the limit switch <NUM> is provided close to an upper portion of the transmission lead screw <NUM> to cause the lifting motor <NUM> to stop operation when the sample stage <NUM> moves upwards to touch the limit switch <NUM>. The position of the limit switch <NUM> is set such that the sample stage <NUM> is located at the detection position thereof when the lifting motor <NUM> stops operation under the trigger of the limit switch <NUM>. The sample stage <NUM> may be kept at the detection position thereof when the lifting motor <NUM> does not operate. In the present application, the detection position of the sample stage <NUM> is positioned by the limit switch <NUM>, the positioning is accurate, and the problem that the sample stage <NUM> exceeds the detection position thereof and continues to move to cause structural damage to the sample stage <NUM>, the microfluidic biochip <NUM>, or the like, can be avoided.

In some embodiments, the sample stage <NUM> may include a support stage <NUM> and an oscillator <NUM>. The support stage <NUM> is used for supporting the sample cup <NUM>. Specifically, the support stage <NUM> may be a horizontally placed support plate, and a groove for placing the bottom of the sample cup <NUM> therein may be provided on the support plate, so as to prevent the sample cup <NUM> from toppling or shaking during the moving process of the sample stage <NUM>, thereby improving the stability of the placement of the sample cup <NUM>. The support stage <NUM> is fixedly connected with the horizontal connecting plate <NUM>.

The oscillator <NUM> is provided on the support stage <NUM>, and is used to oscillate the sample cup <NUM> after the sample cup <NUM> is placed on the support stage <NUM>, such that buffer liquid and a sample in the sample cup <NUM> are fully mixed to generate the sample liquid, thereby fully dissolving a to-be-detected substance on the sample into the buffer liquid to obtain the sample liquid with a suitable concentration. The buffer fluid may be pre-loaded into the sample cup <NUM> by means of manual addition or may be automatically delivered to the sample cup <NUM> by a driving device after the sample cup <NUM> is placed on the sample stage <NUM>.

In some embodiments, the sample stage <NUM> further includes a weighing sensor <NUM>, and the weighing sensor <NUM> is provided below the support stage <NUM> for weighing the weight of the sample in the sample cup <NUM>, thereby allowing a buffer liquid driving device <NUM> to deliver a preset amount of buffer liquid matched with the weight of the sample to the sample cup <NUM>. In general, the sample is extracted at will by a home user, for example, a small vegetable leaf is torn off at will, and therefore, in order to guarantee the accuracy of a measurement result, the quantity of the buffer liquid input into the sample cup <NUM> is required to be matched with the quantity of the sample, so as to generate the sample liquid with a proper concentration. In the present application, the weight of the sample can be automatically and accurately obtained by the weighing sensor <NUM> provided below the support stage <NUM>, such that the buffer liquid driving device <NUM> is automatically controlled to input the matched amount of buffer liquid into the sample cup <NUM>, thus guaranteeing the accuracy of the measurement result, avoiding various problems of inconvenient use, a complex operation, a large error, or the like, caused by manual weighing of the sample by the user, and further improving the automation degree of the microfluidic detection system and the use experience of the user.

It should be noted that, in some alternative embodiments, the sample stage <NUM> may be fixed, and the microfluidic pesticide residue detection chip <NUM> may be configured to be movable, which can also facilitate the sampling operation.

In some embodiments, the microfluidic detection system <NUM> further includes the housing <NUM>. The housing <NUM> is provided with an operation stage <NUM> opened towards the front side thereof, and the sample stage <NUM> is at least partially located in the operation stage <NUM> to facilitate the user to perform operations of placing the sample cup <NUM>, taking out the sample cup <NUM>, or the like, in the operation stage <NUM>. A water disposal pan <NUM> located at a lower portion of the operation stage <NUM> may be provided in the operation stage <NUM> to receive possibly dripping liquid, thereby preventing contamination of the operation stage <NUM>. At least some sections of the microfluidic biochip <NUM>, the detection mechanism <NUM>, a buffer liquid bottle <NUM>, and the buffer liquid driving device <NUM> are arranged in the housing <NUM>. Further, the housing <NUM> is provided with a first structural connecting piece <NUM> for being connected with a cabinet or a door of the refrigerator, and a first electrical connecting piece <NUM> for forming an electrical connection between the microfluidic detection system <NUM> and an electrical control device of the refrigerator <NUM>, so as to allow the microfluidic detection system <NUM> to be mounted to the cabinet or door of the refrigerator as a whole.

In some embodiments, the microfluidic detection system <NUM> further includes the buffer liquid bottle <NUM> and the buffer liquid driving device <NUM>. The buffer liquid bottle <NUM> is provided in the housing <NUM> and is used for containing the buffer liquid. The buffer liquid driving device <NUM> is provided in the housing <NUM> and is communicated with the buffer liquid bottle <NUM> to controllably drive the buffer liquid in the buffer liquid bottle <NUM> into the sample cup <NUM> placed on the sample stage <NUM>, such that the buffer liquid is mixed with the sample in the sample cup <NUM> to generate the sample liquid. Specifically, the buffer liquid bottle <NUM> is communicated with the buffer liquid driving device <NUM> through an inlet pipe <NUM>. An outlet pipe <NUM> of the buffer liquid driving device <NUM> extends to the sample stage <NUM>. This arrangement is adopted mainly for a solid sample as the detected sample, and the buffer liquid is required to dissolve the to-be-detected substance on the solid sample to form the sample liquid; or, the sample is a liquid sample, but has a too high concentration, and the sample is required to be diluted using the buffer liquid to produce the sample liquid. For example, during pesticide residue detection, the detected sample is usually a solid food residue piece, such as a skin, a leaf, or the like, the sample is required to be placed in the buffer liquid, and the pesticide residue on the sample is dissolved in the buffer liquid to form the sample liquid.

Specifically, the buffer liquid driving device <NUM> may be a peristaltic pump, a diaphragm pump or other suitable types of driving devices. The peristaltic or diaphragm pump generates large vibrations in the radial direction thereof when in operation, and in order to prevent the vibrations from being transmitted to the microfluidic biochip <NUM>, an elastic damping piece <NUM> may be provided on the radial outer side of the peristaltic or diaphragm pump. The elastic damping piece <NUM> may be fitted over the buffer liquid driving device <NUM> and supported in the housing <NUM> by the clamping effect of the bracket <NUM> and a fixed block <NUM>, and the fixed block <NUM> may be fixed on the support plate <NUM>.

In some embodiments, the microfluidic detection system <NUM> further includes a circuit board <NUM>, a display device <NUM>, and a switch button <NUM>, the circuit board <NUM> being provided within the housing <NUM> and electrically connected with the first electrical connecting piece <NUM> on the housing <NUM>. The electrical components of the microfluidic detection system <NUM> (for example, the lifting mechanism <NUM>, the buffer liquid driving device <NUM>, the sample liquid driving device <NUM>, the display device <NUM>, the switch button <NUM>, or the like) are all electrically connected to the circuit board <NUM> directly or indirectly. The display device <NUM> is provided on the front side of the housing <NUM> and electrically connected to the circuit board <NUM> for displaying the detection result of the detection mechanism <NUM>. The switch button <NUM> is provided on the front side of the housing <NUM> and electrically connected to the circuit board <NUM> for activating and/or deactivating the detection function of the microfluidic detection system <NUM>. That is, the user can start, pause, or stop the detection function of the microfluidic detection system <NUM> by operating the switch button <NUM>.

In some embodiments, the housing <NUM> may include a rear shell <NUM> at the rear side and a front panel <NUM> connected to the front side of the rear shell <NUM>. An accommodating cavity is defined between the rear shell <NUM> and the front panel <NUM> after the rear shell and the front panel are assembled. The support plate <NUM> and the bracket <NUM> are further provided in the accommodating cavity of the housing <NUM>. The support plate <NUM> is fixedly connected to the rear shell <NUM>, and at least a part of the structure of the lifting mechanism <NUM> (for example, the non-movable part of the lifting mechanism) and the buffer liquid driving device <NUM> are fixed on the support plate <NUM>. The bracket <NUM> is fixedly connected to the front side of the support plate <NUM>, and the microfluidic biochip <NUM> and the sample liquid driving device <NUM> are directly or indirectly supported on the bracket <NUM>. Thus, the lifting mechanism <NUM>, the buffer liquid driving device <NUM>, the microfluidic biochip <NUM>, and the sample liquid driving device <NUM> can be stably supported by the support plate <NUM> and the bracket <NUM> in the accommodating cavity formed between the rear shell <NUM> and the front panel <NUM>.

In some embodiments, the lifting mechanism <NUM> may be provided on the transverse side of the sample stage <NUM>, the buffer liquid driving device <NUM> may be provided on one side of the microfluidic biochip <NUM> in the transverse direction and located above the lifting mechanism <NUM>, the sample liquid driving device <NUM> is located on the other side of the microfluidic biochip <NUM> in the transverse direction, and the buffer liquid bottle <NUM> is located on a side of the sample liquid driving device <NUM> away from the microfluidic biochip <NUM>. For the microfluidic biochip <NUM>, the sample stage <NUM>, the lifting mechanism <NUM>, the buffer liquid driving device <NUM>, the sample liquid driving device <NUM> and the buffer liquid bottle <NUM> with such a layout, the size features of each module in the vertical direction and the transverse direction are fully utilized, such that the layout of the modules is more compact, and the occupied space is reduced as far as possible. Moreover, the modules are only arranged side by side in the vertical direction and the transverse direction, such that the thickness of the microfluidic detection system <NUM> in the front and rear direction is reduced as far as possible, and the microfluidic detection system is more suitable for being integrated on the refrigerator.

Further, a partition <NUM> extending transversely may be provided between the buffer liquid driving device <NUM> and the lifting mechanism <NUM> to avoid that leaked liquid possibly generated by the buffer liquid driving device <NUM> drops on the lifting mechanism <NUM> to affect the normal operation of the lifting mechanism <NUM>. The partition <NUM> may be fixed on the support plate <NUM>.

The present invention further provides a refrigerator, and <FIG> is a schematic structural diagram of the refrigerator according to one embodiment of the present invention. The refrigerator <NUM> according to the present invention includes the microfluidic detection system <NUM> according to any one of the above embodiments, so as to integrate the microfluidic detection system <NUM> on the refrigerator <NUM>. The refrigerator <NUM> is frequently used in daily life, and mainly configured to store food materials, and when the microfluidic detection system <NUM> is integrated on the refrigerator <NUM>, a user can conveniently perform a detection operation of a food material sample by using the microfluidic detection system <NUM>.

Further, the refrigerator <NUM> further includes a cabinet <NUM> and a door <NUM>, the cabinet <NUM> defines a storage space therein, and the door <NUM> is connected to the cabinet <NUM> and configured to open and/or close the storage space. The microfluidic detection system <NUM> is preferably provided on the door <NUM>, such that the operation is convenient, an original storage space in the cabinet <NUM> cannot be occupied, and the storage capacity of the refrigerator <NUM> cannot be influenced.

<FIG> is a schematic exploded structural diagram of the door in one embodiment of the present invention. In some embodiments, a hollowed window <NUM> is provided on the front side of the door <NUM>, and the sample stage <NUM> of the microfluidic detection system <NUM> is exposed on the front side of the door <NUM> through the hollowed window <NUM>, such that the user can be allowed to place the sample cup on the sample stage <NUM> without opening the door <NUM>, thus avoiding the problem that cold leakage is serious due to the door <NUM> being required to be opened during each time of detection, guaranteeing the heat preservation performance of the refrigerator <NUM>, and saving energy consumption.

Specifically, the door <NUM> may include a panel <NUM> for forming a front portion of the door, a door liner <NUM> for forming a rear portion of the door, and a foamed heat insulation layer (not shown) provided between the panel <NUM> and the door liner <NUM>, and the hollowed window <NUM> is formed in the panel <NUM>. A pre-embedded box <NUM> is pre-embedded between the panel <NUM> and the door liner <NUM> before the foamed heat insulation layer is formed, and the microfluidic detection system <NUM> is provided in the pre-embedded box <NUM>. That is, the pre-embedded box <NUM> is pre-provided between the panel <NUM> and the door liner <NUM> before the door <NUM> is foamed, so as to reserve a space for mounting the microfluidic detection system <NUM> between the panel <NUM> and the door liner <NUM>.

Further, the pre-embedded box <NUM> is attached to the rear surface of the panel <NUM>, and the front side of the pre-embedded box <NUM> is open and directly faces the hollowed window <NUM>, such that the microfluidic detection system <NUM> is allowed to be mounted in the pre-embedded box <NUM> from front to back through the hollowed window <NUM>, thus improving the mounting convenience of the microfluidic detection system <NUM>.

Specifically, the pre-embedded box <NUM> can be provided with a second structural connecting piece <NUM> matched and connected with the first structural connecting piece <NUM> and a second electrical connecting piece <NUM> electrically connected with the first electrical connecting piece <NUM>, and the second electrical connecting piece <NUM> is electrically connected with the electrical control device of the refrigerator <NUM>. Thus, the microfluidic detection system <NUM> is mounted on the door <NUM> as a whole by arranging the corresponding structural connecting pieces and electrical connecting pieces on the pre-embedded box <NUM> and the housing <NUM>, such that the whole microfluidic detection system <NUM> is connected with the refrigerator <NUM> in terms of both structure and circuit. Thus, the assembly process of the microfluidic detection system <NUM> is simplified, and the disassembly or maintenance of the microfluidic detection system <NUM> is facilitated.

The refrigerator <NUM> according to the present application is a refrigerator in a broad sense, and includes not only a so-called refrigerator in a narrow sense, but also a storage device having a refrigerating, freezing or other storage function, for example, a refrigerating box, a freezer, or the like.

Claim 1:
A microfluidic detection system (<NUM>) for a refrigerator (<NUM>), comprising:
a microfluidic biochip (<NUM>) that has a sample inlet (<NUM>), a communication port (<NUM>) and a detection pool (<NUM>) formed therein, wherein the sample inlet, the detection pool and the communication port communicate in sequence by means of a microfluidic channel (<NUM>);
a sample liquid driving device (<NUM>), which is in sealed communication with the communication port by means of a sealed docking mechanism (<NUM>), and is used to impel sample liquid in contact with the sample inlet to enter the microfluidic channel and flow to the detection pool by means of the microfluidic channel; and
a detection mechanism (<NUM>), which is used for detecting the detection pool so as to obtain a preset detection parameter of the sample liquid,
wherein the communication port of the microfluidic biochip is fixedly provided with a plug pin (<NUM>) protruding and extending outward, an internal flow channel (<NUM>) of the plug pin is in sealed communication with the communication port, and the plug pin is inserted into the inside of the sealed docking mechanism and forms a fluid-tight connection with the sealed docking mechanism, and the sealed docking mechanism is in fluid-tight connection with the sample liquid driving device, so that the sample liquid driving device is in sealed communication with the communication port;
characterized in that an end surface (<NUM>) of an extended tip of the plug pin is a continuous and smooth hemispherical surface, and a pin hole (<NUM>) of the plug pin for fluid communication with the sealed docking mechanism is formed on the circumferential side (<NUM>) of a section of the plug pin located inside the sealed docking mechanism.