Patent Description:
In study fields of genetic engineering and protein engineering technologies, a nucleic acid molecule is a main subject of study, and isolation and linearization of nucleic acid are basic technologies of nucleic acid research. The nucleic acid is a basic unit representing genetic characteristics of a life entity, and nucleic acid detection is advanced biological detection performed in a molecular level and has remarkable advantages of high sensitivity, high specificity, no window period and so on, compared with a conventional morphological detection, cytological detection, immunological detection and the like. The nucleic acid detection includes technologies such as qualitative polymerase chain reaction (PCR), molecular hybridization, real-time fluorescence quantification PCR and so on, and first critical aspects of these nucleic acid detection technologies are to complete extraction of the nucleic acid of a biological sample. Therefore, effectively and accurately extracting a nucleic acid template becomes a premise of subsequent nucleic acid detection. At present, nucleic acid extraction in China mainly adopts a conventional manual manufacture and preparation method, which is low in efficiency, high in cost, and poor in repeatability and stability.

<CIT> shows an apparatus with a pipetting mechanism and an heater for a sample in a row of wells. <CIT> shows a heating plate for well plate with an adapted form for the wells.

An objective of the present invention is to provide a nucleic acid extraction instrument and particularly to a fully automatic nucleic acid extraction instrument.

The nucleic acid extraction instrument of the present invention includes a base, an outer housing connected with the base, an instrumental main body positioned inside the outer housing and mounted to the base. The instrumental main body includes an electrical power pack, a main control device, a first motor set, a second motor set, and a third motor set.

Further, the first motor set may output a movement in a direction approximately parallel to a certain plane of the base, and the second motor set and the third motor set may output a movement in a direction approximately perpendicular to the plane of the base.

Further, the first motor set may be fixed to the base, the first motor set may include a motor fixed on the base and incapable of generating a relative displacement relative to the base, a ball screw coaxially connected with a shaft of the motor via a connector, a sliding block connected to the ball screw, and a guide rail playing a guiding role for the sliding block.

Further, the second motor set and the third motor set may be fixed to a sliding block of the first motor set and reciprocate along an axial direction of the motor under a drive of the first motor set.

Further, the second motor set and the third motor set may be fixed to the sliding block of the first motor set via a motor bracket. The motor bracket may include a first panel and a second panel, which are approximately perpendicular to each other. The first panel may be configured to fix the second motor set and the third motor set, and the second panel may be fixed to the sliding block on the first motor set.

Further, when the sliding block reciprocates along an axial line of the ball screw, the sliding block may drive the motor bracket, the second motor set, and the third motor set to reciprocate between a first position and a second position in a plane parallel to a bottom plate and a lengthwise direction of an operating platform.

Further, the nucleic acid extraction instrument may further include a bottom plate, an inner housing, a portal frame, and an operating platform, which are fixed to the base.

As shown in <FIG>, a nucleic acid extraction instrument <NUM> in the present invention includes a base <NUM> positioned at a bottom, an outer housing <NUM> positioned on the base, and an instrumental main body <NUM> positioned inside the outer housing <NUM> and fixed to the base <NUM>. The base <NUM> is made of a material (such as cast iron, steel, a concrete-filled steel plate and so on) with relatively high specific weight and low cost, so that the base has a relatively high mass, thereby effectively preventing the nucleic acid extraction instrument from vibrating and moving in a running process. The outer housing <NUM> is preferably made of a stainless steel plate with a thickness of <NUM>-<NUM> processed and manufactured by techniques such as cold rolling, cold cutting and so on. In other solutions, the outer housing <NUM> may also be made of a plastic material manufactured by injection molding to reduce the cost. A plurality of mutually parallel or crisscross tendons or ribs may be designed on the outer housing <NUM> to improve the strength of the outer housing <NUM>. Preferably, according to different selected materials, the outer housing <NUM> may be treated by techniques such as paint spraying, computer inkjet, computer carving, etching, electroplating and so on, so that the outer housing has a better appearance. The side of the outer housing <NUM> facing an operator, i.e. the front side of the nucleic acid extraction instrument <NUM>, is designed with an instrumental panel <NUM> and a door <NUM> capable of being opened or closed for multiple times. A display screen and a plurality of operating keys may be designed on the instrumental panel <NUM>, thus to facilitate operation by the operator. Preferably, a touch liquid crystal display screen is designed on the instrumental panel <NUM>, thereby facilitating the operator to operate by directly touching the liquid crystal display screen.

As shown in <FIG>, the instrumental main body <NUM> includes a bottom plate <NUM> fixed to the aforementioned base <NUM>, an inner housing <NUM>, a portal frame <NUM>, an operating platform <NUM>, a power pack <NUM>, a main control device <NUM>, a first motor set <NUM>, a second motor set <NUM>, and a third motor set <NUM>. The bottom plate <NUM> provides at least one plane for supporting the aforementioned assembly. Preferably, the bottom plate <NUM> is a steel plate with a thickness of <NUM>-<NUM>, which should have a moderate rigidity and can keep at least one face be of a plane shape without easily being bent, twisted, partially upwarped and the like. To save the cost, under a condition of meeting the above requirements, the bottom plate <NUM> may be made of hard plastic by an injection molding process or may be made of hard wood or bamboo by a woodworking process. In one embodiment, when the bottom plate <NUM> has a sufficient thickness, rigidity, and mass, the bottom plate <NUM> may also serve as the base <NUM>. Wherein, in one preferred embodiment, the inner housing <NUM>, the portal frame <NUM>, the operating platform <NUM>, the power pack <NUM>, and the first motor set <NUM> are fixed on the bottom plate <NUM>, respectively. To save a space, the main control device <NUM> is disposed on a back face of the power pack <NUM> (as shown in <FIG>). As shown in <FIG> and <FIG>, the first motor set <NUM> is fixed on an upper surface of the bottom plate <NUM>, and a lengthwise direction or an axial line of the first motor set <NUM> is parallel to the upper surface of the bottom plate. The second motor set <NUM> and the third motor set <NUM> are fixed to a motor bracket <NUM> at first and then installed on the first motor set <NUM> by the motor bracket <NUM>. The second motor set <NUM> and the third motor set <NUM> are arranged adjacently and in parallel, axial lines of the second motor set <NUM> and the third motor set <NUM> are mutually parallel and are perpendicular to the upper surface of the bottom plate <NUM>, respectively. A guide rail or a slide track <NUM> is designed on the portal frame <NUM>, a sliding block <NUM> is designed at the motor bracket <NUM> corresponding to the guide rail <NUM>, and the sliding block <NUM> is limited to slide in the guide rail <NUM>. Such a design can not only improve the stability of the motor bracket <NUM> in movement but also improve a protection function for the motor bracket <NUM> by the nucleic acid extraction instrument of the present invention in transporting and conveying processes. In one implementing mode, the portal frame <NUM> includes at least one cross beam <NUM> and two vertical beams <NUM> and <NUM> for supporting the crossbeam <NUM>. Wherein the guide rail <NUM> is designed on the cross beam <NUM>, and the vertical beams <NUM> and <NUM> are fixed to the bottom plate <NUM> or the upper surface of the base <NUM>, respectively.

As shown in <FIG>, the operating platform <NUM> includes a flat and approximately cuboid-shaped operating panel <NUM> adjacent to a front face of the nucleic acid extraction instrument <NUM>. The operating panel <NUM> should have suitable rigidity and strength and cannot be deformed easily. The operating panel <NUM> may be made of a stainless steel plate by a cold working process or may be made of hard plastic by an injection molding process. The operating panel <NUM> includes a platform approximately parallel to the upper surface of the bottom plate <NUM>, and operations of extracting nucleic acid are basically completed on this platform. The platform includes two lengthwise sides approximately parallel to the front face of the nucleic acid extraction instrument <NUM>. When the door <NUM> is in an open state, the operator can enter from the door <NUM> with two hands or one hand, the operations of extracting the nucleic acid are performed on the operating platform <NUM>. The first motor set <NUM> is installed behind the operating platform <NUM> (that is a position further away from the front face of the nucleic acid extraction instrument than the operating platform <NUM>, referring to <FIG> and <FIG>). The lengthwise direction or an axial direction of the first motor set <NUM> is approximately parallel to a lengthwise side of the operating platform <NUM>.

The power pack <NUM> includes a component (unmarked) for converting a <NUM> V or <NUM> V general voltage into a voltage suitable for driving various electronic components inside the nucleic acid extraction instrument of the present invention and at least one set of power supply management assembly <NUM>. To save the space, the power supply management assembly <NUM> is fixed above the power pack <NUM> (as shown in <FIG> and <FIG>). Please refer to <FIG>, the power supply management assembly <NUM> includes at least one printed circuit board <NUM>, a plurality of electronic components such as capacitors and resistors, at least one power supply regulator <NUM>, and a heat radiator <NUM>. The heat radiator <NUM> is closely against the power supply regulator <NUM>. The power supply regulator <NUM> includes a heat emitting electronic component for converting an instantaneous overload voltage or overload current possibly generated during a running process of the nucleic acid extraction instrument into heat energy to be consumed. The heat radiator <NUM> is configured to timely transmit the heat energy generated by the power supply regulator <NUM>, thereby cooling the power supply regulator <NUM> to avoid burning the power supply regulator <NUM> due to excessive temperature.

As shown in <FIG>, the motor bracket <NUM> includes a first panel <NUM> and a second panel <NUM> which are approximately perpendicular to each other, wherein the second motor <NUM> and the third motor set <NUM> are fixed on the first panel <NUM>, and the second panel <NUM> is fixed to the first motor set <NUM>. The motor bracket <NUM> may be made of a steel plate by a cold working process or may be made of hard plastic by an injection molding process. The motor bracket <NUM> is integrally approximately of an "L" shape. To improve the strength of the motor bracket <NUM>, save materials, and reduce the weight, at least one tendon or rib <NUM> is disposed at the corner where the first panel <NUM> and the second panel <NUM> form an angle of approximately <NUM>°. As shown in <FIG> and <FIG>, the first motor set <NUM> includes a motor <NUM> which is fixed on the upper surface of the bottom plate <NUM> and cannot generate a relative displacement relative to the bottom plate <NUM>, a ball screw <NUM> coaxially connected with a shaft of the motor <NUM> via a connector <NUM>, a sliding block <NUM> connected to the ball screw <NUM>, and a guide rail <NUM> playing a guiding role for the sliding block <NUM>. When the first motor <NUM> works, the shaft of the motor <NUM> drives the ball screw <NUM> by the connector to rotate along an axial line thereof. The ball screw <NUM> rotates along the axial line to drive the sliding block <NUM> to reciprocate along the axial line of the ball screw <NUM>. Because the second panel <NUM> of the motor bracket <NUM> is fixed to the sliding block <NUM> of the first motor set <NUM>, when the sliding block <NUM> reciprocates along the axial line of the ball screw <NUM>, the sliding block <NUM> drives the motor bracket <NUM>, the second motor set <NUM>, and the third motor set <NUM> reciprocate between a position "A" and a position "B" in a plane parallel to the bottom plate <NUM> and a lengthwise direction of the operating platform <NUM> (as shown in <FIG>).

As shown in <FIG>, the nucleic acid extraction instrument in the present invention includes a magnetic bar frame <NUM> fixedly connected to a sliding block of the second motor set <NUM> and a magnetic bar sleeve frame <NUM> fixedly connected to a sliding block of the third motor set <NUM>. The magnetic bar frame <NUM> is downward provided with a plurality of rows of magnetic bars <NUM>, and each row has a plurality of magnetic bars (preferably <NUM>*<NUM> magnetic bars). The magnetic bar sleeve frame <NUM> is downward provided with channels <NUM> with a number (such as four) corresponding to the number of the rows of the magnetic bars. One magnetic bar sleeve may be inserted in each channel <NUM>.

<FIG> show a running process of a nucleic acid extraction instrument <NUM> in the present invention. When the nucleic acid extraction instrument <NUM> is in a starting position, the magnetic bar frame <NUM> is separated from the magnetic bar sleeve frame <NUM> and is spaced a sufficiently large distance from the operating platform <NUM>. The nucleic acid extraction instrument <NUM> in the present invention sequentially runs according to the following steps. A first step: adding a sample -a deep well plate (also named as <NUM>-well plate) in which the sample is added is placed at a specified position of the operating platform <NUM>, and then the magnetic bar sleeves <NUM> are fixed to the channels <NUM> of the magnetic bar sleeve frame <NUM>. A second step: lysing- the third motor set <NUM> is started to allow the magnetic bar sleeve frame <NUM> to drive the magnetic bar sleeves <NUM> to descend until the magnetic bar sleeves <NUM> enter a specified row of wells of the deep well plate <NUM>, the third motor set <NUM> then drives the magnetic bar sleeve frame <NUM> and the magnetic bar sleeves <NUM> together to vibrate for a preset time according to a frequency selected from (<NUM>-<NUM>) Hz (preferably, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM>), the ends, which are far away from the magnetic bar sleeve frame <NUM>, of the magnetic bar sleeves <NUM> stir a mixture of the sample, a lysing solution, and magnetic balls (placed in the wells of the deep well plate in advanced) in the wells of the deep well plate <NUM>, so that nucleic acid in the sample is separated from the sample (that is, the nucleic acid is lysed) and adsorbed on the magnetic balls. A third step: attracting-the second motor set <NUM> is started and drives the magnetic bar frame <NUM> to descend until the magnetic bars <NUM> enter specified positions of the magnetic bar sleeves <NUM> to rest for a preset time, so that all the magnetic balls are attracted on outer surfaces of the magnetic bar sleeves <NUM> together with the nucleic acid lysed from the sample, and then the magnetic bar frame <NUM> and the magnetic bar sleeves <NUM> ascend together to ensure that the magnetic bar sleeves <NUM> and the magnetic bars <NUM> enter a specified row of washing wells of the deep well plate <NUM> for washing to remove impurities. A fourth step: eluting-the magnetic bar frame <NUM> and the magnetic bar sleeves <NUM> enter into eluting wells from the washing wells together, and the nucleic acid is released from the magnetic balls under an action of an eluting solution. A fifth step: recovering the magnetic balls-the magnetic balls attracted on the outer surfaces of the magnetic bar sleeves <NUM> are transferred into magnetic ball recovering wells from the eluting wells, and the magnetic bars <NUM> leave from the magnetic bar sleeves <NUM>. A sixth step: discarding the magnetic balls-the magnetic bar sleeves <NUM> vibrate in the magnetic ball recovering wells, so that the magnetic balls all fall off from the outer surfaces of the magnetic bar sleeves <NUM>. A seventh step: cleaning-discharging the magnetic bar sleeves <NUM>, removing the deep well plate, and returning the instrument to the starting position. Wherein, in the lysing and eluting steps, the sample mixed solution in the deep well plate is required to be heated to reach a preset temperature range.

As shown in <FIG>, <FIG>, and <FIG>, the first motor set <NUM>, the second motor set <NUM>, and the third motor set <NUM> are approximately identical in structures, and each adopts a step motor and a ball screw which have a high precision. A repeating precision (that is, a displacement error made by each cycle) of the step motors adopted by the present invention is less than <NUM>. As described above, the first motor set <NUM> is used for driving the second motor set <NUM> and the third motor set <NUM> to move between the first position and the second position according to a preset program. The second motor set <NUM> is used for driving the magnetic bar frame <NUM> to move upward or downward. The third motor set <NUM> is used driving the magnetic bar sleeve frame <NUM> to move upward or downward and vibrate. Because the third motor set <NUM> needs to drive the magnetic bar sleeve frame <NUM> to vibrate at a high frequency, the higher the vibrating frequency is, the larger the impulse of the inertia generated by the mass of the magnetic bar sleeve frame per se to the third motor set <NUM> is, and the larger the interference is. Therefore, the probability of out of step of the motor (under one pulse, the case that the displacement error output by the step motor exceeds a design value is called as the out of step of the motor) is also larger. To avoid the motor to step out, a coder <NUM> is disposed at the third motor set <NUM> according to the present invention. The coder <NUM> includes a component for monitoring a running condition of the step motor and a component for sending a correction instruction to the step motor. When an error between a stroke generated after the step motor receives one pulse and a pre-designed value is greater than a preset value (such as <NUM> micrometers), the coder sends one correction instruction to timely correct the stroke output by the step motor according to each pulse, thereby avoiding the out of step of the motor in advance and improving the precision that the third motor set <NUM> drives the magnetic bar sleeve frame <NUM> to vibrate.

In addition to the running precision of the motor sets, factors influencing a movement precision of the magnetic bar sleeve frame <NUM> and the magnetic bar frame <NUM> further include an initial positioning precision of the magnetic bar sleeve frame <NUM> and the magnetic bar frame <NUM> and a position precision of the maximal stroke. As such, a technical solution adopted by the present invention is as follows: a photoinduction arm <NUM> is fixed at an outer side of the sliding block <NUM> of each motor set, and a photocoupling sensor <NUM> is respectively disposed at an initial position and a maximal stroke position of the sliding block <NUM>. The photocoupling sensor <NUM> monitors the position of the sliding block <NUM> by detecting the position of the photoinduction arm <NUM>. Therefore, after the photocoupling sensor <NUM> is adopted, the first motor set <NUM> can accurately control the positions of the second motor set and the third motor set (that is, the magnetic bar sleeve frame <NUM> and the magnetic bar frame <NUM>) corresponding to the positions where lysing, washing, eluting, and recovering of the magnetic balls are performed in the deep well plate <NUM>; and the second motor set and the third motor set can accurately control the lowest position and the highest position of the magnetic bar sleeve frame <NUM> and the magnetic bar frame <NUM>.

As shown in <FIG>, a plurality of rows of movable heating plates <NUM> is disposed on the operating platform <NUM>. Each row of the heating plates <NUM> includes a plurality of heating well walls <NUM> closely matching the shape of the bottom of the deep well plate <NUM>. The heating plates <NUM> are preferably made of a metal material, a heating component (unmarked) such as a resistance wire is disposed inside the heating plates <NUM> in a penetrating manner, and a heat insulating component (unmarked) is disposed around the heating plates <NUM>. A plurality of mutually independent compression springs <NUM> is disposed under the heating plates <NUM> to promote all the heating well walls <NUM> of the heating plates <NUM> to attach to the bottom of the deep well plate <NUM> more uniformly, thereby making each well heated more uniformly. This greatly improves the consistency of lysing and eluting efficiencies of the samples in respective wells in the deep well plate <NUM>, thereby greatly reducing an error of nucleic acid extraction efficiencies in respective wells.

As shown in <FIG>, <FIG>, <FIG>, and <FIG>, the operating platform <NUM> includes at least one set of clamping tools for automatically clamping and releasing the deep well plate <NUM> placed on the operating platform <NUM>. The clamping tools includes motors <NUM> (the number of the motors corresponds to the number of the deep well plates) positioned below the bottom plate <NUM> (that is, a back face of the operating platform <NUM>), a supporting portion <NUM> positioned inside the operating platform <NUM>, a plurality of clamping arms <NUM>, and a microswitch <NUM>. The clamping arms <NUM> include a shaft <NUM>, an up-down movement of the supporting portion pushes the clamping arms <NUM> to rotate around the shaft <NUM>, clamping heads <NUM> of the clamping arms tightly clamp or release the deep well plate <NUM>. The motor <NUM> includes a cam <NUM>. When the motor <NUM> is started, the cam <NUM> pushes the supporting portion <NUM> to move upward or downward. The supporting portion <NUM> further pushes the clamping arms <NUM> to rotate clockwise or anticlockwise according to a lever principle, and the clamping arms <NUM> tightly clamp or release outer walls of the deep well plate <NUM>. Torsional springs (unmarked) are disposed at the clamping arms, and when no deep well plate <NUM> is placed on the operating platform <NUM>, the torsional springs make the clamping arms keep a releasing state to reduce the obstruction of placing the deep well plate <NUM> onto the operating platform <NUM>.

As shown in <FIG>, <FIG>, <FIG>, <FIG>, <FIG>, <FIG>, <FIG>, the microswitch <NUM> is positioned near one end of the operating platform <NUM>. The microswitch <NUM> includes a spring leaf <NUM> and a button switch <NUM>. <FIG> is a view showing that the microswitch is in a turning-off state, and <FIG> is a view showing that the microswitch is in a turning-on state. When the deep well plate <NUM> is placed to a predetermined position of the operating platform <NUM>, side walls of the deep well plate <NUM> push the spring leaf <NUM> to compress the button switch <NUM>, thereby turning on the microswitch <NUM> to start the motor <NUM> to work. The motor <NUM> drives the cam <NUM> to push the supporting portion <NUM> to move upward. The supporting portion <NUM> further pushes the clamping arms <NUM> to rotate according to the lever principle to make the clamping arms <NUM> tightly clamp the outer walls of the deep well plate <NUM>, thereby precisely fixing the deep well plate <NUM> to a position on the operating platform <NUM>. When there is a need for removing the deep well plate <NUM> from the operating platform <NUM> after the whole process of extracting the nucleic acid finishes, the operator sends an instruction of releasing the deep well plate <NUM> by the instrumental panel <NUM>. The motor <NUM> drives the cam <NUM> to rotate, and the supporting portion <NUM> moves downward under the action of springs and self-gravity. Under the action of the torsional springs, the clamping arms <NUM> reversely rotate to return to a state of releasing the deep well plate <NUM> and do not exert a clamping force to the deep well plate <NUM>. Therefore, the operator can easily remove the deep well plate <NUM> from the operating platform <NUM>.

As shown in <FIG>, <FIG> and <FIG>, a cross flow fan <NUM> used for radiating heat to outside of the instrument is disposed at the end, close to the instrumental panel <NUM>, of the operating platform <NUM>. The cross flow fan <NUM> is hidden below the operating panel <NUM> of the operating platform <NUM>. An air outlet of the cross flow fan <NUM> is positioned in the bottom of the nucleic acid extraction instrument of the present invention.

As shown in <FIG>, <FIG> and <FIG>, the instrumental main body <NUM> of the nucleic acid extraction instrument in the present invention is basically enclosed by the base <NUM> and the outer housing <NUM>, while the bottom of the base <NUM> is provided with an air outlet for the cross flow fan <NUM>. The nucleic acid extraction instrument of the present invention is further provided with the inner housing <NUM> and the operating panel <NUM>. The inner housing <NUM> and the operating panel <NUM> basically limit the deep well plate <NUM> in a relatively closed space. The above structure design is very beneficial to preventing an external environment from influencing the cleanness inside the instrument.

As shown in <FIG>, <FIG>, the door <NUM> of the nucleic acid extraction instrument <NUM> in the present invention includes a door handle <NUM> and a door shaft <NUM>. Wherein, the outer housing <NUM> is provided with a permanent magnetic block (not illustrated) at a position corresponding to the door handle <NUM>, and when the door <NUM> is closed, the permanent magnetic block attracts the door handle <NUM>, thereby making the door <NUM> kept in a closed state. The door shaft <NUM> includes a door middle shaft <NUM> fixed to the base <NUM> and door shaft sleeves <NUM> fixed to two ends of the bottom of the door <NUM>. Two ends of the door middle shaft <NUM> are assembled into the door shaft sleeves <NUM>, respectively, and door shaft torsional springs <NUM> are disposed at the positions where the door middle shaft <NUM> is joined with the door shaft sleeves <NUM>. The door shaft torsional springs <NUM> enable joints between the door middle shaft <NUM> and the door shaft sleeves <NUM> to keep having a certain damping coefficient, thereby increasing a hand feeling when the door <NUM> is opened or closed and improving a pleasant feeling of the operator.

<FIG> shows a working principle and steps of the nucleic acid extraction instrument in the present invention. A first step: adding a sample (such as whole blood) into the deep well plate. A second step: heating and lysing the sample under a mechanical vibration of the magnetic bar sleeves. A third step: making the magnetic bars enter into the magnetic bar sleeves and adsorbing the magnetic balls and nucleic acid. A fourth step: washing away the impurities under an action of the washing solution. A fifth step: eluting the nucleic acid from the magnetic bar sleeves under an action of an eluting solution. A sixth step: recovering the magnetic balls. A seventh step: removing the magnetic bars from the magnetic bar sleeves and making the magnetic balls fall off from the magnetic bar sleeves under an action of the mechanical vibration of the magnetic bar sleeves and fall into the magnetic ball recovering wells.

<FIG> shows steps for fixing the deep well plate and heating the sample by the nucleic acid extraction instrument in the present invention. A first step: placing the deep well plate. A second step: triggering the microswitch. A third step: responding to a CPU signal. A fourth step: starting the motor. A fifth step: moving the supporting portion upward. A sixth step: tightly clamping by the clamping arms. A seventh step: making the heating plates tightly attach to the deep well plate.

Claim 1:
A nucleic acid extraction instrument (<NUM>) for extracting a nucleic acid, comprising: a base (<NUM>), an outer housing (<NUM>) connected with the base, an instrumental main body (<NUM>) positioned inside the outer housing (<NUM>) and mounted to the base (<NUM>); wherein the instrumental main body (<NUM>) comprises an electrical power pack (<NUM>), a main control device (<NUM>), a first motor set (<NUM>), a second motor set (<NUM>), and a third motor set (<NUM>),
the nucleic acid extraction instrument (<NUM>) further comprising:
an operating platform (<NUM>); and
a plurality of rows of movable heating plates (<NUM>) disposed on the operating platform (<NUM>); wherein each of the rows of movable heating plates (<NUM>) includes a plurality of heating well walls (<NUM>) closely matching the shape of a bottom of a deep well plate (<NUM>); further wherein a plurality of mutually independent compression springs (<NUM>) is disposed under the movable heating plates (<NUM>).