Patent Description:
MALDI-analysis is a useful tool for solving structural problems in biochemistry, immunology, genetics and biology. Samples are ionized in the gas phase and a time of flight (TOF) analyzer is used to measure ion masses. TOF analysis begins when ions are formed and are accelerated to a constant kinetic energy as they enter a drift region. They arrive at a detector following flight times that are proportional to the square root of their masses. A mass spectrum is created because ions of different mass arrive at the detector at different times.

Mass spectrometry can be a powerful tool in the fields of drug discovery and development, genotyping, and proteome research. In addition MALDI has already been used for characterization and identification of bacteria and microorganisms. Current trends in research are to analyze larger and larger numbers of samples using quantities of individual samples ranging from the micro-mole levels to ato-mole levels. As a result, samples are also becoming smaller and needs exists for efficient and reliable acquisition of the correct amount of micro-organisms and accurately depositing a sample of the acquired amount on a target plate used in the MALDI-instrument.

In a typical MALDI TOF MS operation, the sample to be analyzed is spotted or deposited on a metal plate (also called target plate or MALDI-plate), reagents are added (matrix) that support ionization, and then they are dried to form crystals. In these instruments, the target plate is positioned in a fixed position in the MALDI-instrument. The target plate has a plurality of depositing spots (e.g. from <NUM> to <NUM> depositing spots on a single target plate) and these depositing spots have a fixed orientation with regard to the edges of the target plate. The target plate is positioned on an X-Y stage so that an obtained sample of a colony of microorganisms can be deposited on a selected depositing spot. A high voltage potential is maintained between the target plate and a metal grid. This voltage can be maintained or pulsed, depending upon the desired results and a vacuum is created in the chamber. A laser is fired into the sample/matrix and a plume of ions is formed. The voltage difference is used to accelerate the ions up a flight tube so that they can be analyzed. The analysis directly relates the time of flight to the mass of the ionized component.

Several parameters can affect the quality of the results, including flatness of the target, amount and type of matrix, concentration of the sample, conductivity of the sample target, accuracy of placement on the depositing spot, as well as other variables.

In particular, an important aspect is the handling of the sample and the concentration of the sample. It is known that a suspension is made from a sample of a colony of microorganisms and that a researcher pipettes a drop of the obtained suspension containing the sample onto a depositing spot of the target plate by hand. To provide a correct analysis, however, the suspension has to contain a sufficient concentration of the sample from the start.

In preparing such a suspension of a sample of microorganisms, a handheld device comprising a drive. The drive comprises a housing with a rotary drive motor contained therein, and a connector which is configured for the releasable attachment of the sample acquisition device to the drive. The sample acquisition device comprises a sample collection region which is first brought into contact with biological material (mostly grown on a culture dish) to be analyzed. Thereafter the sample collection region is attached to the rotary drive, brought into contact with a liquid medium contained in a tube and the rotary drive is activated for a certain period of time such that a sample of the biological material in the sample acquisition device is released in the liquid medium. After the sample collection region is removed from the tube the suspension tube holds a suspension containing the sample which for example can be used for performing MALDI-analysis.

However, the efficiency of release of the sample from the sample acquisition region can in some cases be insufficient to perform a correct analysis of the microorganism suspended in the liquid medium. This may result in a prepared suspension that is unusable, resulting in lost of time and money. In addition since the sample collection region is rotated within the tube the tube must be of a sufficient dimension to allow such rotation without the sample acquisition region contacting the inner wall of the tube which might be detrimental for the release of the microorganism in the liquid medium. Such a relative large dimensioned tube thus contains a relatively large volume of liquid medium, leading to a corresponding lengthy time of further processing such liquid suspension medium. For example, the time in which the liquid suspension containing the sample has to incubate in an incubator is proportional to the amount of suspension liquid. Therefore, there is a need for a method in which the preparation of a suspension of a sample of microorganisms is performed automatically leading to a much more reproducible manner of preparing such a suspension. In addition there is a need for reliably and reproducibly releasing the sample on a depositing spot of a target plate.

By way of background information, <CIT> discloses a method and system for harvesting bacteria to determine a minimum inhibitory concentration (MIC) of an antibiotic during culture. According to <CIT>, the bacterial colony is collected at a calculated volume to avoid repeatedly diluting a suspension or adding additional bacteria to the suspension.

In order to solve at least one of the problems mentioned above the present invention provides a method according to claim <NUM>,in which an initial amount of a suspension medium is dispensed into a suspension tube in a suspension tube holder. A first picking tool (<NUM>) in a transferring device is used to automatically introduce a sample of microorganisms into the suspension. The picking tool is oscillated in a vertical movement for a period of time, after which the turbidity of the suspension is measured. In response to the measured turbidity, the method does at least one of: a) dispense an additional amount of the suspension medium into the suspension tube; b) obtain and release a further sample of the microorganisms in the suspension tube, followed by more vertical oscillation; or c) provide, with a controller, an indication that the suspension tube is ready for use. It appears that the problems mentioned above for the greater part find their cause therein that the steps are performed manually and these steps are therefore prone to undesired variance and mistakes, leading to incorrect results from the MALDI-instrument, additional costs and loss of time. By automating each of the steps these problems can be overcome to at least a large extent. In the present field it has been taken for granted that at least some of the steps only could be performed manually, however in contrast hereto the present invention provides the possibility for the first time to automate all the steps necessary for locating and selecting a colony of microorganisms and identifying microorganisms in said selected colony using MALDI.

The disclosure will be further explained with reference to <FIG>, in which a non-limiting exemplary apparatus is shown, which <FIG> will also be used to explain exemplary embodiments of the disclosed methods.

The first picking tool (<NUM>) as defined in the description corresponds to the second picking tool (<NUM>) as defined in the appended set of claims. The second or further picking tool (<NUM>) as defined in the description corresponds to the first picking tool (<NUM>) as defined in the appended set of claims. In an example not falling under the scope of the claimed invention, MALDI or MALDI-TOF-MS is used to identify microorganisms. In a MALDI TOF MS operation a sample of a colony of microorganisms is spotted or deposited on a target plate which is held in a fixed position in the MALDI-instrument. Such a target plate has a plurality of depositing spots (e.g. from <NUM> to <NUM> depositing spots on a single target plate) and these depositing spots have a fixed orientation with regard to the edges of the target plate. The target plate is positioned on an X-Y stage so that an obtained sample of a colony of microorganisms can be deposited on a selected depositing spot, wherein the location where a specific sample has been deposited is indicated by the X-Y parameters and is stored in a memory of a central control computer.

Although not depicted in detail in <FIG>, the target plate is positioned below a transfer track <NUM> at a position indicated by B. A sample can be transferred along the transfer track <NUM> from a culture dish <NUM> and/or a suspension tube <NUM> to above the target plate at position B, where the sample is lowered to be deposited on a depositing spot of the target plate.

Although the disclosure will be described detailed below with reference to preparing a suspension containing a sample and depositing a drop of said suspension on a depositing spot of a target plate, the present disclosure also relates to directly depositing a sample obtained (picked) from a culture dish onto a depositing spot of a target plate.

In general, in the latter method a colony of microorganisms is automatically located and detected on a culture dish. A sample of said selected colony of microorganisms is obtained in an automated way, e.g. by a picking tool which is brought into contact with the colony. At least some of said sample of said selected colony of microorganisms is directly deposited on a target plate in an automated way, by lowering the picking tool such that the sample of the colony of microorganisms present on the picking tool comes into contact with the target plate, and moving the picking tool in a plane parallel to the plane of the target plate such that the sample of the colony of microorganisms is deposited on the depositing spot, in particular covering at most approximately half of said one of the depositing spots of the target plate. Thereafter the target plate with said sample is automatically transferred into an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. The embodiment described in this paragraph recites directly depositing a picked colony onto a depositing spot on a target plate and is not part of the present invention.

More detailed this method comprises the following automated steps:.

In case the same colony of microorganisms is to be subjected to another analysis or test, such as but not limited to an Antibiotic Susceptibility Test (AST) a second sample of said selected colony of microorganisms can be obtained in a manner similar to obtaining the initial sample. Since the location of the colony of microorganisms on the culture dish has already been selected, and is therefore "known" to the central control computer, it is possible to easily and reproducibly obtain a second sample from the same colony. At least some of the second sample of said selected colony of microorganisms is then transferred and deposited on a test culture dish, which can for example be automatically positioned at another position below the transfer track <NUM>. Thereafter said test culture dish is automatically transferred to an apparatus for performing a susceptibility test or another additional analysis. The above paragraph describes a step in which a picked colony is directly deposited onto a test culture dish and is not part of the present invention.

Since for performing characterization and identification of microorganisms normally a plurality of colonies is grown on a culture dish, and in addition a plurality of different culture dishes is used, the disclosure provides the possibility to identify each culture dish separately, e.g. by means of a bar code, and furthermore each colony of interest on a single culture dish is selected and given an identification mark. Hereto before the automated step of locating and selecting a colony of microorganisms on a culture dish, the method according to the disclosure comprises a step of providing a culture dish comprising a number of colonies of microorganisms, obtaining an initial image of said culture dish including all the colonies of microorganisms, displaying said initial image of said culture dish including all the colonies of microorganisms on a display, and selecting at least one colony of microorganisms in said initial image. In this manner a researcher or analyst can select colonies of interest based on thorough education and knowledge. Since each culture dish is provided with an individual identification identifying said culture dish, such as a bar code, the initial image of said culture dish including all colonies is stored, and information regarding said at least one selected colony of microorganisms is stored (preferably with links given in the (electronic) initial image), storing all said information and identifications of the culture dish in a memory of a central control computer allows a very correct further processing. In this manner the only manual operation is the act of selecting the interesting colonies, whereas all relevant data are processed in an automated way. Optionally the researcher or analyst can manually enter processing instructions regarding the processing to which a selected colony of microorganisms of said culture dish is to be subjected, said processing instructions being also stored in said memory of said central control computer for later use. After this manual act all the further steps are performed completely automated in a reliable and efficient manner. For this automated further processing the culture dish is automatically positioned on a stage for a culture dish of a picking tool device comprising an imaging device. An image of said culture dish positioned in said picking tool device is obtained, and together with the identification of said culture dish it is possible to compare this image obtained by the imaging device of said picking tool device with the stored initial image of said culture dish and thus derive information regarding the location of the selected colony of microorganisms and optionally regarding the processing instructions regarding the processes to be performed on said selected colony of microorganisms. By comparing the image of the culture dish when it is placed in the picking tool device with the initial image, the location of the selected colonies can be obtained automatically, for example by computerized image comparison. Furthermore each target plate is provided with an identifying mark and optionally each depositing spot of said target plate has an individual identification mark or location identifier, so that after storing the identifying mark of said target plate and depositing spots together with the properties of the suspension with a link to the identity of the culture dish from which the selected colony of microorganisms was obtained in the memory of said central control computer a correct linking of the obtained MALDI results to the specific colony of microorganism under test is possible in a correct and automated way. The above paragraph describes a method for automatically identifying and selecting a plurality of colonies of microorganisms and is not part of the present invention.

Disclosed herein is a method in which a suspension is made from a sample of a colony of microorganisms picked from a culture dish together with an apparatus for performing such a method.

<FIG> schematically shows an apparatus <NUM> for the automatic preparation of a suspension of a sample of a microorganism according to the disclosure. Said apparatus <NUM> comprises a stage <NUM> for a culture dish <NUM> comprising a microorganism <NUM> on a nutritional layer <NUM>, such as a layer of agar gel.

The apparatus <NUM> further comprises a first picking tool <NUM> and a further picking tool <NUM>. A positioning device <NUM> comprises a picking tool holder <NUM> for, in the as shown in <FIG> releasably holding a picking tool, as shown in <FIG> the picking tool holder <NUM> holds the first picking tool <NUM>. The positioning device <NUM> is arranged for positioning the first picking tool <NUM> in a starting position (shown in solid lines in <FIG>) above the culture dish <NUM> and is arranged for automatically lowering and raising the first picking tool <NUM> towards and away from the culture dish <NUM>, such that the first picking tool <NUM> can be positioned in a position (indicated with broken lines <NUM>') in which it contacts the microorganism <NUM> and picks up a sample of said microorganism. After the first picking tool <NUM> has picked up a sample the positioning device <NUM> raises and positions the first picking tool <NUM> in a transfer position, which, as shown in <FIG>, may be identical to the starting position. Or the starting and transfer positions may be different from each other.

The apparatus <NUM> further comprises a suspension tube holder <NUM> for holding a suspension tube <NUM> which can contain a suspension medium which is dispensed from an automatic suspension medium dispenser <NUM>, which, as shown in <FIG>,has a dispensing nozzle <NUM> for automatically dispensing a suspension medium <NUM> in the suspension tube <NUM> held in the suspension tube holder <NUM>. The suspension tube holder <NUM> may be a rotatable suspension tube holder for rotating the suspension tube <NUM> around a vertical axis A.

A transferring device <NUM> is incorporated in the apparatus <NUM> for automatically transferring a picking tool from the transfer position of the positioning device <NUM> to a position above the suspension tube <NUM> held in the suspension tube holder <NUM>. As shown in <FIG>, the transferring device <NUM> comprises a transfer holder <NUM> with a grasping means <NUM> for releasably holding a picking tool. The transferring device <NUM> may in a manner known per se be mounted on a transfer track <NUM>, such as a rail, for linear movement thereon as indicated by the arrows. In this manner the transferring device <NUM> may be moved to the positioning device <NUM>, such that the grasping means <NUM> can take over the picking tool from the positioning device <NUM>, the picking tool holder <NUM> thereof releasing the picking tool after the grasping means <NUM> has grasped the picking tool. As shown in <FIG> the second or further picking tool <NUM> having previously picked up a sample <NUM> of the microorganism <NUM> is positioned above the suspension tube <NUM> by the transferring device <NUM> in a starting position indicated by solid lines. The transferring device <NUM> is arranged for lowering the second picking tool <NUM> into the suspension medium <NUM> contained in the suspension tube <NUM>, in which position the second picking tool <NUM>' with the sample <NUM> is submerged in the suspension medium <NUM> as indicated by broken lines in <FIG>. In this position the transferring device <NUM> is activated for oscillating the second picking tool <NUM> in a linear vertical movement for a period of time which is sufficient for the sample <NUM> to be released from the second picking tool <NUM>. Thereafter the transferring device <NUM> positions the second picking tool <NUM> in a waiting position above the suspension tube <NUM>, which waiting position is , as shown in <FIG>, identical to the starting position or the waiting position and the starting position may be different from each other.

The apparatus <NUM> further is provided with a turbidity meter <NUM> for performing measurements of the turbidity of the suspension medium <NUM> contained in the suspension tube <NUM> held in the suspension tube holder <NUM>. As generally known in the art a turbidity meter can provide measurement values which are a measure of the concentration of material, in the present case the concentration of a microorganism suspended in the suspension medium. As shown in <FIG> the turbidity meter <NUM> may comprise a laser <NUM> which transmits laser light towards and through the suspension medium and a sensor <NUM> which detects the amount of laser light transmitted through the suspension medium. In addition there is a further sensor (not indicated in the drawing) which is e.g. arranged perpendicular to the path of the laser light to detect the amount of laser light which has been scattered by the suspension.

The operation of the device is controlled by a controller <NUM>, for example comprising a microprocessor, which is communicatively connected ( as indicated by the signal lines) to the positioning device <NUM>, the transferring device <NUM>, the automatic suspension medium dispenser <NUM>, and the turbidity meter <NUM> for automatically controlling the movement of the positioning device <NUM>, the movement of the transferring device <NUM>, the operation of the automatic suspension medium dispenser <NUM> and the operation of the turbidity meter <NUM>, respectively. In addition the controller <NUM> might be directly communicatively connected to other parts of the apparatus such as for example the picking tool holder <NUM>, the transfer holder <NUM>, the laser <NUM> and the sensor <NUM>.

As shown in <FIG>, the controller <NUM> is arranged for controlling the turbidity meter <NUM> such that the turbidity measurement of the suspension medium <NUM> is started before the second picking tool <NUM> is submerged in the suspension medium <NUM>. In addition the controller <NUM> controls the rotatable suspension tube holder <NUM> for starting the rotation of the suspension tube <NUM> held in the holder <NUM> before the second picking tool <NUM> is submerged in the suspension medium <NUM>, and for maintaining the rotation of the suspension tube <NUM> during the measurement of the turbidity of the suspension medium <NUM>. As shown in <FIG>, the controller <NUM> controls the turbidity meter <NUM> such that the measurement of the turbidity is performed during the total period of time during which the second picking tool <NUM> is oscillated. In this manner the turbidity meter <NUM> provides an on-line measurement value to the controller <NUM> which value is indicative of the measured turbidity, and thus the concentration of the microorganism, during the period of time during which the second picking tool is oscillated.

The controller <NUM> comprises a memory in which a first and a second threshold value are stored, in which said first threshold value is equal to or greater than the second threshold value. If the turbidity measurement value provided by the turbidity meter is equal to or between the first and second threshold value, the concentration/amount of microorganism in the suspension medium is sufficient to allow the suspension tube with the suspension to be further processed. In that case the controller <NUM> provides a signal that the suspension tube can be processed further. In addition in this situation the second picking tool <NUM> can be discarded e.g. by transferring the transferring device to a position C in which the grasping means <NUM> are activated to release the second picking tool <NUM>.

In case the final measurement value of the turbidity meter is above the first threshold value previously stored in a memory of the controller <NUM> then the concentration of the microorganism is too high to allow the suspension tube to be processed further. In that situation the controller <NUM> controls the automatic suspension medium dispenser <NUM> to supply an additional amount of suspension medium into the suspension tube <NUM>. This additional amount of suspension medium is based on the initial amount of suspension medium, the final measurement value and the value of the first and/or second threshold value such that the addition of the additional amount of suspension medium to the suspension medium already present in the suspension tube <NUM> will lead to a concentration of microorganism in the suspension medium which satisfies the requirement for further processing, as can be confirmed by an additional or further measurement of the turbidity by the turbidity meter <NUM>.

In case the final measurement value of the turbidity meter <NUM> is below the second threshold value, meaning that the concentration of microorganism in the suspension medium is too low, the controller <NUM> controls the apparatus <NUM> such that an additional sample of microorganism is picked up by the first picking tool <NUM> (alternatively the second or another picking tool can be used for picking up an additional sample). Thus the controller <NUM> in this case controls the positioning of the transfer device <NUM> such that the second picking tool <NUM> is discarded as described above. Then (or simultaneously) the first picking tool <NUM> in the picking tool holder <NUM> of the positioning device <NUM> is lowered from the starting position above the culture dish <NUM> towards the culture dish and into contact with the microorganism <NUM> to pick up an additional sample of said microorganism. Thereafter the first picking tool <NUM> is automatically raised with the additional sample of the microorganism away from the culture dish to the transfer position. Then said transferring device automatically transfers the first picking tool with the additional sample of the microorganism from the transfer position of the positioning device <NUM> to a position above the suspension tube <NUM>. The first picking tool <NUM> with the additional sample of the microorganism is lowered into the suspension medium <NUM> and is oscillated by the transferring device <NUM> in a linear vertical movement for a period of time for releasing the additional sample of said microorganism in the suspension medium. Again the turbidity is measured during the oscillation, and the measured value is compared with the first and second threshold value stored in the memory of the controller <NUM>. In this case the controller <NUM> can be arranged for controlling the movement of the transferring device <NUM> such that the first picking tool <NUM> is raised to the waiting position if during oscillation the on-line measurement value of the turbidity performed by the turbidity meter <NUM> is equal to or lower than the first threshold value and equal to or greater than the second threshold value.

Suspension tubes which are particularly useful in the apparatus have a substantially circular cross-section with a diameter of about <NUM> to about <NUM>, preferably about <NUM>. In these relatively small suspension tubes the controller <NUM> can control the automatic suspension medium dispenser <NUM> such that the supplied initial amount of suspension medium is about <NUM>,<NUM> - <NUM>, preferably about <NUM>.

The oscillation of the transferring device <NUM> is controlled by the controller <NUM> such that the picking tool oscillates at a frequency between about <NUM> to about <NUM>, preferably about <NUM> to about <NUM>, most preferably about <NUM>, with an amplitude of about <NUM> to about <NUM>, preferably about <NUM> to about <NUM>. The controller is furthermore arranged for controlling the oscillation of the transferring device <NUM> such that the period of time during which the picking tool oscillates is about <NUM> seconds to about <NUM> seconds, preferably about <NUM> seconds.

The apparatus <NUM> in addition comprises a conveyor <NUM> of which the end position can form the stage <NUM> for the culture dish or as shown in <FIG> a conveyor <NUM> and a stage <NUM> which are mutually positioned such that a culture disk can be transported onto the stage and removed from the stage by appropriate operation of the conveyor <NUM>. The conveyor <NUM> is controlled by the controller <NUM> for automatically positioning and removing a culture dish comprising said microorganism on and from the stage, respectively. Please note that different, not shown, means for automatically positioning and removing a culture dish on and from the stage, respectively, can be used. In particular the controller <NUM> is arranged for allowing a culture dish to be automatically removed from the stage by the automatic culture dish positioning and removing device only after the signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing has been provided. This ensures that it is always possible to pick up an additional sample, if necessary.

As shown in <FIG> the apparatus <NUM> further comprises an automatic suspension tube positioning and removing device <NUM> for automatically positioning and removing a suspension tube in and from the suspension tube holder, respectively. As shown in <FIG>, automatic suspension tube positioning and removing device <NUM> comprises grasping means <NUM> for releasably grasping a suspension tube <NUM>'. Again, the controller <NUM> is arranged for being communicatively connected to the automatic suspension tube positioning and removing device <NUM>, <NUM> for controlling the operation of the automatic suspension tube positioning and removing device, and for automatically positioning a suspension tube in the suspension tube holder. The controller <NUM> in particular is arranged for automatically removing a suspension tube holder from the suspension tube holder by the automatic suspension tube positioning and removing device <NUM>, <NUM> only after the signal that the suspension tube with the suspension can be removed from the suspension tube holder for further processing has been provided. As shown in <FIG> the automatic suspension tube positioning and removing device <NUM> is movable along the rail <NUM> independent of the movement of the transferring device <NUM>. In the position indicated by C empty suspension tubes can be fetched and suspension tubes with a suspension medium containing a sufficient concentration of microorganism can be handed over to equipment for further processing, such as an incubator. Please note that the position C can for example be formed by a multitrack system which can lead the suspension tube positioning and removing device <NUM> and the transferring device <NUM> to different location at which different components are present or processes can be performed.

The sample suspension thus prepared is used for performing characterization or identification of the microorganisms using MALDI and optionally used for other analysis, such as AST. For identifying microorganisms using MALDI a drop of said sample suspension is obtained; and this drop is transferred onto said target plate. A drop can be obtained by using another picking tool which is held by the grasping means <NUM> and which is automatically lowered in the suspension. When this picking tool is raised out of the suspension a drop of suspension will stick on the tip of this picking tool, which can be transferred along the track to position B, where the picking tool with the drop is lowered until the drop contacts the depositing spot on the target plate, and at least a part of the drop will remain on the depositing spot after the picking tool has been raised away from the target plate. Alternatively a pipetting tool, to be described below, can be used to pick up an amount of suspension from the suspension tube, transfer this amount to position B and deposit a drop of suspension on the target plate. After a drop of suspension has been deposited on the target plate, and in particular when this drop has been allowed to dry, a drop of a MALDI matrix solution is automatically overlaid on the amount or portion of the sample deposited on the target plate. For performing other tests or another analysis a second drop of said sample suspension can be obtained in a similar way, and such a drop may be automatically transferred to and deposited on e.g. a test culture dish which is further transferring in an automated way for performing a susceptibility test or another additional analysis.

Each suspension tube comprises an unique identifying mark, which is stored together with the properties of the suspension with a link to the identity of the culture dish from which the selected colony of microorganisms was obtained in the memory of said central control computer for the purpose of amongst other things correctly and in a fast manner link the obtained results of analysis with the culture dish and colony pertaining to said results.

Claim 1:
A method for automatically preparing a suspension of a sample of microorganisms, the method comprising:
dispensing, with an automatic suspension medium dispenser (<NUM>), an initial amount of a suspension medium (<NUM>) into a suspension tube (<NUM>) held in a suspension tube holder (<NUM>); transferring, with a transferring device (<NUM>), a sample of a colony of microorganisms (<NUM>) from a first picking tool (<NUM>) to the suspension tube (<NUM>) by submerging the first picking tool (<NUM>) in the suspension medium (<NUM>), wherein the first picking tool (<NUM>) is releasably held in a grasping means (<NUM>) of a transfer holder (<NUM>) of the transferring device (<NUM>);
oscillating, with the transferring device (<NUM>), the first picking tool (<NUM>) in a linear vertical movement for a period of time;
after the period of time during which the first picking tool (<NUM>) is oscillated has elapsed, measuring, with a turbidity meter (<NUM>), the turbidity of the suspension medium (<NUM>) contained in the suspension tube (<NUM>); and
depending on the measured turbidity, (a) dispensing, with the automatic suspension medium dispenser (<NUM>), an additional amount of the suspension medium (<NUM>) into the suspension tube (<NUM>), (b) obtaining a further sample of the colony of microorganisms (<NUM>) with the first picking tool (<NUM>) or a second picking tool (<NUM>) releasably held in the grasping means (<NUM>) of the transfer holder (<NUM>) of the transferring device (<NUM>) and releasing the further sample of the colony of microorganisms (<NUM>) in the suspension tube (<NUM>) by vertically oscillating the first picking tool (<NUM>) or the second picking tool (<NUM>), or (c) providing, with a controller, an indication that the suspension tube (<NUM>) is ready for further use.