Patent Description:
Microscopy is widely used in research as well as in industrial applications. Spectral data of microscopic objects is often of interest and extending the spectral range of microscopy applications from the visible part of the spectral range into covering also the short-wave infrared, SWIR, range can often provide valuable information. However, extending the spectral range of microscopy instrumentation to span from visible up to SWIR is challenging because of the varying behavior of detectors and optical components across the wide spectral range.

Imaging detectors typically have a good sensitivity only in a limited spectral range, either dedicated for imaging in the visible range or the infrared range. Consequently, instrumentation intended to cover the range from visible to SWIR typically requires two different imaging detectors, one for visible and one for infrared. These systems require switching between the two detectors in order to cover the full range. Because of differences in pixel pitch and the numbers of pixels between the detectors, it is challenging, and sometimes impossible, to achieve a perfect pixel-to-pixel correspondence of the field-of-view for the two detectors. This is further complicated by difficulties in positioning the two detectors at the exact same location, especially in microscopic applications in which even very small shifts may be significant to cause problems. Further, also the microscope objectives need to be switched between applications in the visible and the SWIR part of the range. This introduces differences in chromatic aberrations, zoom, and other imaging distortions. Especially in applications where imaging in both the visible and the SWIR range of the same object is required, all these artefacts need to be dealt with, for example by digital image processing such as resampling, rescaling, and/or dewarping, in order to align the spatial information in the respective images.

Moreover, the need for replacing components when switching between different wavelength ranges is time consuming. As a result, the work flow of the personnel working with the instrumentation is slowed down. Furthermore, the need for replacing components also limits the data acquisition rate, and this limitation is especially pronounced in applications where data acquisition in both the visible and SWIR is needed for the same object.

The increased number of components further results in bulky and costly instrumentation. Therefore, there is a need in the art for an improved solution for imaging of microscopic objects covering the spectral range from visible to SWIR. The document "<NPL> discloses a known device for imaging a microscope object in SWIR.

An objective of the present inventive concept is to mitigate, alleviate or eliminate one or more of the above-identified deficiencies in the art and disadvantages singly or in any combination. These and other objects are at least partly met by the invention as defined in the independent claims.

According to a first aspect of the present inventive concept there is provided a device for imaging of a microscopic object, the device comprising:.

The term "imaging" of a microscopic object is herein regarded to include also acquisition of the interference pattern. By acquisition of the interference pattern formed by interference between the scattered light from the microscopic object and the non-scattered light, information about the three-dimensional shape of the microscopic object is acquired in terms of a digital hologram. Such information enables generation of a visual image of the microscopic object, by means of holographic reconstruction.

By the term "light sensitive area" is here meant an area reacting to light impinging onto the area, by generating an electrical signal as a response to the light intensity. Arrays of light sensitive areas may be arranged on detectors, configured to allow read-out of the detected light for image acquisition. Given as non-limiting examples, light sensitive areas may be found on photodiodes, photo-multiplier tubes (PMT), and pixels on image sensors such as charge-coupled devices (CCD) and complementary metal oxide semiconductors (CMOS). By way of example, an array of light sensitive areas may be in the form of pixels on a CCD or CMOS sensor, but may alternatively comprise a plurality of any other type of light sensitive areas. Given as other non-limiting examples, an array of light sensitive areas may be of quantum dot image sensor type.

By the phrase "sensitive to detect light spanning a wavelength range" is here meant that the light sensitive area, as a reaction to light impinging onto the area, has the capability of generating an electrical signal as a response to light intensity for light of all wavelengths that fall within the defined wavelength range. Further, the light sensitive area may or may not be sensitive to light of wavelengths that fall outside of the defined wavelength range. A light sensitive area is considered sensitive to light of a certain wavelength if the light sensitive area has a quantum efficiency of <NUM>% or greater, for that wavelength. It should be understood that if a light sensitive area is sensitive to detect light of a specific wavelength and a spectral filter blocking the wavelength is arranged in front of the light sensitive area, such that light with that specific wavelength is prevented from reaching the light sensitive area, the light sensitive area as such is still regarded as being sensitive to the specific wavelength.

In this context the term "light" should be allowed a wider interpretation, not limited to visible electromagnetic radiation but may also include for example ultra-violet light and infra-red light.

By the "visible" part of the wavelength spectrum is here meant electromagnetic radiation within the wavelength range of <NUM>-<NUM>.

By the "infrared (IR)" part of the wavelength spectrum is here meant electromagnetic radiation within the wavelength range of <NUM> to <NUM>.

By the "near infrared (NIR)" part of the wavelength spectrum is here meant electromagnetic radiation within the wavelength range of <NUM>-<NUM>.

By the "short wave infrared (SWIR)" part of the wavelength spectrum is here meant electromagnetic radiation within the wavelength range of <NUM>-<NUM>.

By the term "light source" is here meant any unit, device and/or element at which light is generated. By way of example, the light source may be, but is not limited to a laser, a laser diode, a light emitting diode, or a combination thereof.

Generation of light at a plurality of wavelengths within the wavelength range may be accomplished by a single light source being configured to generate all the wavelengths. Generation of light at a plurality of wavelengths within the wavelength range may alternatively be accomplished by a number of light sources combined, wherein each light source is configured to generate light at one or a few wavelengths within the wavelength range, each of the light sources being configured to generate light at different wavelengths such that light at the plurality of wavelengths is generated by the combination of the number of light sources. It is conceivable that the one or more light sources may be configured to generate light also at other wavelengths not comprised within the plurality of wavelengths.

By the term "scattered light" is here meant elastically scattered light, thus light scattered such that the direction and/or phase and/or intensity of light may change but the energy of the photons, and thus the wavelength of the light is substantially unchanged, apart from a slight Doppler shift that may result from the movement of the sample. In other words, the scattering process does not involve any net energy transfer between the light and the scattering sample, in terms of e.g. change in electronic energy states of the atoms or molecules in the sample. Given as non-limiting examples, the elastic scattering may be, but is not limited to, Rayleigh or Mie scattering.

It is an insight of the inventive concept that imaging of microscopic objects over a wide range of wavelengths may be improved by using an array of light sensitive areas sensitive to a wide range of wavelengths and using detection of interference patterns to avoid microscope objectives or other optical components in an imaging system. This implies that a common setup may be used over a wide range of wavelengths.

An advantage with the present inventive concept is that with an array of light sensitive areas sensitive to detect light spanning the full range of intended use of the device, the need for switching between different arrays of light sensitive areas in order to detect light from the visible part to the SWIR part of the wavelength range, is eliminated.

Another advantage with the present inventive concept is that a microscope objective is not required in order to detect the interference pattern, thereby also eliminating the need for switching between different microscope objectives or other optical components in order to detect light from the visible part to the SWIR part of the wavelength range. Further, aberrations otherwise introduced in the imaging path by optical components are eliminated as well. Moreover, since a microscope objective is not required, the present inventive concept allows for the device to be made compact as well as at a relatively lower cost than objective-based devices. Thus, by the present arrangement, a compact, low cost device for aberration-free, multi-spectral imaging of microscopic objects covering the full spectral wavelength range without the need for switching components when switching between the different wavelengths, may be provided.

Another advantage is that no re-alignment is required during operation, since the same array of light sensitive areas may be used for detection of interference patterns for all wavelengths. This may facilitate combining information for different wavelengths into a multi-spectral image of the microscopic object. This also allows for a more user-friendly device to be provided.

Consequently, with no requirement of replacing components during operation throughout the intended wavelength range of the device, a device with increased acquisition speed may be provided. Further, by the present arrangement, simultaneous detection at the plurality of wavelengths may be enabled.

According to an embodiment, the device may be further configured to detect the interference patterns at the plurality of wavelengths such that spatial information of the microscopic object in the interference patterns for each wavelength in the plurality of wavelengths are aligned with each other on the array of light sensitive areas, enabling combination of the information from each of the plurality of wavelengths into multi-spectral information of the microscopic object.

By the spatial information of the microscopic object in the interference patterns for each wavelength in the plurality of wavelengths being aligned with each other is here meant that, once the images of the microscopic object have been reconstructed, the spatial positions of the microscopic object with respect to the array of light sensitive areas match for the plurality of wavelengths. In other words, the images acquired at the plurality of wavelengths may be superimposed with one another such that corresponding parts of the microscopic object will overlap. By way of example, such alignment may be achieved by, aligning the light at the plurality of wavelengths, from the at least one light source, onto a common path prior to being directed towards the microscopic object. Given as non-limiting examples, light at the plurality of wavelengths may be aligned onto a common path by means of dichroic mirrors or an arrangement of optical fibers.

An advantage with this embodiment is that the monochromatic images acquired at the plurality of wavelengths show essentially the same perspective of the microscopic object. Thus, image transformation, such as resampling, rescaling, and dewarping, is not required in order to align the information in the respective images. By the present arrangement superimposing of the monochromatic images for combining them into a multi-spectral image may be facilitated.

According to an embodiment, the at least one light source is configured to generate light having a spectral line-width equal to or less than <NUM> and being at least partially coherent, for each wavelength of the plurality of wavelengths.

Coherent light may be advantageous as it improves the interference visibility. A coherent light source may be a laser. However, it should be understood that also partially coherent light may provide an interference pattern with sufficient visibility. A partially coherent light source may e.g. be a light emitting diode, LED, emitting light through a pinhole onto the microscopic object. A coherent light source may provide better interference visibility but may be more expensive while a partially coherent light source may provide a worse interference visibility but may be less expensive. It should further be realized that temporal coherence of the generated light is not important. Thus, the generated light may be at least partially spatially coherent, but need not be temporally coherent. The at least partially spatially coherent light may create an interference pattern, formed by interference between light scattered by the microscopic object and non-scattered light, at the array of light sensitive areas.

A narrow spectral line-width may be an advantage because it may facilitate matching the line-width of the light from the light source with the spectral band or bands of interest to probe or measure at.

Another advantage of a narrow spectral line-width is that, if the plurality of wavelengths is acquired sequentially one by one, the requirement for spectral filtering in front of the array of light sensitive areas may be eliminated.

According to an embodiment, the at least one light source comprises one or more lasers and/or one or more laser diodes and/or one or more light emitting diodes, LEDs.

According to an embodiment, the array of light sensitive areas is of Indium Gallium Arsenide, InGaAs, type.

An advantage with this embodiment is that light sensitive areas of InGaAs type may provide relatively high quantum efficiency within the full spectral range in which the devices is intended to operate. High quantum efficiency may provide improved image quality of the detected interference patterns.

Another advantage with this embodiment is that it may allow for the use of lower power light sources than otherwise required in order to provide good image quality of the detected interference patterns.

According to an embodiment, the array of light sensitive areas is of quantum dot image sensor type.

An advantage with this embodiment is that light sensitive areas of quantum dot image sensor type may provide relatively high quantum efficiency within the full spectral range in which the devices is intended to operate. High quantum efficiency may provide improved image quality of the detected interference patterns.

Yet another advantage with this embodiment is that the wavelength range in which the quantum dots are sensitive to light can be tuned within the visible and infrared wavelength range. Such tuning may be achieved by selection of material and particle size, thereby adjusting what wavelength range is absorbed by the quantum dots. In the present manner, quantum dot image sensors may be tailor-made to match the desired wavelength range for visible/SWIR applications.

Yet another advantage with this embodiment is that it may provide a small pitch of the light sensitive areas. By small pitch of the light sensitive areas, higher image resolution of the acquired interference patterns may be provided.

According to an embodiment, a pitch of the light sensitive areas in the array of light sensitive areas is not larger than <NUM>.

By the term "pitch" is here meant the distance from a center of a light sensitive area to the center of a consecutive light sensitive area. In other words, the pitch includes a width of a light sensitive area as well as a separation between two consecutive light sensitive areas. The resolution of the acquired images is related to the pitch of the light sensitive areas. In order to obtain images with high spatial resolution, the pitch of the light sensitive areas needs to be sufficiently small. By way of example, arrays of light sensitive areas with a pitch of the light sensitive areas of <NUM>, preferably <NUM>, and more preferably <NUM> or smaller may be suitable for use in the device for imaging of microscopic objects.

According to an embodiment, the device is further configured to, by the at least one light source, sequentially illuminate the microscopic object with the generated light at the plurality of wavelengths one by one, and, by the array of light sensitive areas, sequentially detect the interference patterns for imaging the microscopic object at the plurality of wavelengths.

The array of light sensitive areas may acquire image frames, each image frame corresponding to a single exposure of the array of light sensitive areas. The interference pattern for light at a single wavelength may be detected in each image frame. Thus, the interference patterns at the plurality of wavelengths may be detected and acquired in consecutive image frames. The present embodiment is suitable for imaging stationary or quasi-stationary microscopic objects, where movements of the microscopic object are so slow that the microscopic object may be considered stationary (movement is smaller than size of a pixel) during the sequential acquisition of the plurality of wavelengths.

An advantage with this embodiment is that as the microscopic object is illuminated by light at individual wavelengths one by one, and thus the individual interference patterns are detected and acquired one by one, the acquisition may utilize full resolution of the array of light sensitive areas for each of the plurality of wavelengths. By the present arrangement high resolution images may be provided for each of the plurality of wavelengths.

Another advantage with this embodiment is that, since the microscopic object is illuminated by light at individual wavelengths one by one, leakage of information between the interference patterns at different wavelengths may be avoided.

According to an embodiment, the device is further configured to, by the at least one light source, simultaneously illuminate the microscopic object with the generated light at the plurality of wavelengths, and, by the array of light sensitive areas, simultaneously detect the interference patterns for imaging the microscopic object at the plurality of wavelengths.

The interference patterns for the plurality of wavelengths may thus be detected and acquired in the same image frame.

An advantage with this embodiment is that it may provide a significantly faster acquisition of all of the plurality of wavelengths, since all of the plurality of wavelengths are acquired at the same time. Increased image acquisition speed makes the present embodiment suitable for imaging stationary as well as non-stationary microscopic objects.

According to an embodiment, an array of filters is arranged on the array of light sensitive areas, wherein the array of filters comprises a plurality of subsets of filters, each of the filters in the subset of filters being arranged in front of a light sensitive area, wherein each of the subsets of filters is configured to transmit light at one of the plurality of wavelengths, and wherein each of the subsets of filters are further configured to transmit light at a different wavelength than other subsets, such that each of the wavelengths of the plurality of wavelengths is transmitted through a corresponding subset of filters.

It should be understood that with the present embodiment, not all of the light sensitive areas of the array of light sensitive areas are utilized for detecting the interference pattern at a single wavelength. Rather, light at a specific wavelength only reaches a subset of light sensitive areas such that not the full resolution of the array of light sensitive areas is used for each of the plurality of wavelengths.

By way of example, the filters in the array of filters may be, but are not limited to, interference filters or absorption filters or a combination thereof. Given as non-limiting examples, the filters in the array of filters may be made of thin films, absorbing materials or nanoparticles.

An advantage with the present embodiment is that it allows simultaneous detection of all of the plurality of wavelengths by a single array of light sensitive areas. By detection of all of the plurality of wavelengths using a single array of light sensitive areas, a more compact device for imaging of microscopic objects may be provided. Further, by the present arrangement, capability of acquiring images of a full volume with a single acquisition may be provided.

According to an embodiment, the device may further comprise a processor configured to perform digital holographic reconstruction on the interference patterns detected by the array of light sensitive areas to generate a monochromatic image of the microscopic object at each of the wavelengths of the plurality of wavelengths.

The processor may be arranged internally in the device. Alternatively, the processor may be arranged in an external unit, such that the digital holographic reconstruction may take place elsewhere. Data of the interference patterns detected by the array of light sensitive areas may be transferred to the processor, either by a wired connection or wirelessly. As yet another alternative, the processor for performing digital holographic reconstruction may be distributed among physical units, such that parts of the digital holographic reconstruction may be performed in different physical units.

It should be understood that any suitable algorithm for performing the digital holographic reconstruction may be used, as known to the person skilled in the art, including a Gerchberg-Saxton algorithm or multi-acquisition (multi-depth and/or multi-wavelength) for phase retrieval, or reconstruction based on angular spectrum diffraction by means of Gabor wavelet transform. Further, algorithms for performing digital holographic reconstruction may include theories of diffraction, such as Fresnel, Fraunhofer and or Kirchhoff theories, or may be based on deep machine learning methods.

According to an embodiment, the processor is further configured to combine the monochromatic images of the microscopic object generated for each of the wavelengths of the plurality wavelengths, thereby forming an aligned multi-spectral image of the microscopic object.

The non-scattered light from the light source may be passed along a common light path with the light being scattered by the microscopic object. Thus, the interference pattern may be formed within a wavefront passing the microscopic object in a so-called in-line holography set-up. However, according to an alternative, the non-scattered light may be passed along a separate reference light path, which is combined with the light having been scattered by the microscopic object for reaching the array of light sensitive areas.

An advantage is that the in-line holographic set-up may be realized by a simple arrangement. Such a set-up may be preferred as an optical measurement method since it may be robust. Further advantages are that such a set-up may be compact and realized at low cost.

According to a second aspect of the present inventive concept there is provided a method for imaging a microscopic object, the method comprising:.

Effects and features of the second aspect are largely analogous to those described above in connection with the first aspect. It is further noted that the inventive concepts relate to all possible combinations of features unless explicitly stated otherwise.

In cooperation with attached drawings, the technical contents and detailed description of the present inventive concept are described thereinafter according to a preferable embodiment, being not used to limit the claimed scope. This inventive concept may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided for thoroughness and completeness, and fully convey the scope of the inventive concept to the skilled person.

<FIG> illustrates a device <NUM> for imaging microscopic objects <NUM>, according to an embodiment of the inventive concept. The device comprises a light source <NUM>, which in the present embodiment is a laser configured to generate light at a plurality of wavelengths within a wavelength range of at least <NUM>-<NUM>, including wavelengths in the visible part as well as in the SWIR part of the spectrum.

The light source <NUM> may be tunable for selecting a desired wavelength within the wavelength range. Thus, the light source <NUM> may be configured to sequentially output different wavelengths within the wavelength range. The light source <NUM> may for instance be a tunable laser diode.

The light source <NUM> is configured such that the spectral line-width for each of the generated wavelengths does not exceed <NUM>, in order to avoid spectral overlap between the generated wavelengths. It should be realized that other spectral line-widths of the generated wavelengths may be provided, such as spectral line-widths that do not exceed <NUM> or spectral line-widths that do not exceed <NUM>. In particular, when the light source is a laser, a narrow spectral line-width may be provided.

The light from the light source <NUM> is guided by an optical fiber <NUM> to an output <NUM>. The output <NUM> is arranged such that the light exiting the output <NUM> is directed towards a microscopic object <NUM>. In this embodiment, an objective slide <NUM> is used on which microscopic objects <NUM> are arranged, and the microscopic objects <NUM> are thereby illuminated on the objective slide <NUM> from a first side of the objective slide <NUM>.

It should be understood that, although the light source <NUM> is herein described as a laser, it is conceivable that the light source may alternatively be a laser diode or a light emitting diode, LED.

As the microscopic objects <NUM> are illuminated, at least part of the light is scattered by the microscopic objects <NUM>, forming scattered light, whereas some of the light is non-scattered and passes through the microscopic objects <NUM> and the objective slide <NUM>. The scattered and non-scattered light is transmitted to a detector <NUM> comprising an array of light sensitive areas <NUM>, the detector <NUM> being arranged on a second side of the objective slide <NUM>, opposite to the first side. At the detector <NUM>, an interference pattern is formed by interference between light being scattered by the microscopic objects <NUM> and non-scattered light from the light source <NUM>. By the present arrangement, non-scattered light may be transmitted along a common light path with the light scattered by the microscopic objects <NUM>, in a so called in-line holography set-up. In alternative embodiments, however, scattered light and non-scattered light may be transmitted along separate light paths, being combined at the array of light sensitive areas <NUM> to form the interference pattern. Also, it should be realized that the array of light sensitive areas <NUM> may alternatively be arranged on the side of the microscopic objects <NUM> and the objective slide <NUM> from which the microscopic objects <NUM> are illuminated, with light transmitted through the microscopic objects <NUM> being reflected by a mirror towards the array of light sensitive areas <NUM> such that a reflective set-up is used instead of a transmissive set-up.

The array of light sensitive areas <NUM> is configured to detect the interference pattern, for each wavelength of the plurality of wavelengths. Each of the light sensitive areas <NUM> are sensitive to detect light spanning the wavelength range of at least <NUM>-<NUM>, thereby being able to detect light in the full range of wavelengths produced by the light source <NUM>.

The array of light sensitive areas <NUM> of the detector may selectably be of either InGaAs type or quantum dot image sensor type. However, it should be realized that the array of light sensitive areas <NUM> may be formed by another image sensor type that is sensitive to detect light spanning the wavelength range of at least <NUM>-<NUM>.

As the spatial resolution of the detected interference patterns is related to the pitch of the light sensitive areas <NUM> in the array of light sensitive areas <NUM>, it is preferable to use a detector <NUM> with an array of light sensitive areas <NUM> having a small pitch, not larger than <NUM>. Provided as non-limiting examples, suitable detectors may have a pitch of <NUM>, <NUM>, or <NUM>.

The device <NUM> is configured to, by the light source <NUM>, sequentially illuminate the microscopic objects <NUM> with the generated light at the plurality of wavelengths one by one. The device <NUM> is further configured to, by the array of light sensitive areas <NUM>, sequentially detect the interference patterns for imaging the microscopic objects <NUM> at the plurality of wavelengths. More specifically, the device <NUM> is configured such that each illumination event of the light source <NUM> is synchronized with a corresponding detection event of the array of light sensitive areas <NUM>. In an alternative embodiment, the microscopic objects <NUM> may be illuminated by light at all of the plurality of wavelengths simultaneously, and the interference patterns of light at all of the plurality of wavelengths may be detected simultaneously. Given as a non-limiting example, the interference patterns at the plurality of wavelengths may be separated by means of an array of filters arranged in front of the array of light sensitive area <NUM>, thereby allowing simultaneous detection of the individual interference patterns by the same detector <NUM>. Such an array of filters is described in more detail in relation to <FIG>.

In the present embodiment, the light source <NUM> generates light at the plurality of wavelengths and the light at the plurality of wavelengths exit the light source through a common exit and the plurality of wavelengths are pre-aligned on a common light path. The illumination therefore follows the same path for each of the plurality of wavelengths. Further, no objective is used for imaging that may cause chromatic aberrations, and the interference patterns for each of the plurality of wavelength are detected by the same detector <NUM> with the array of light sensitive areas <NUM>. Hence, the device <NUM> is configured to detect the interference patterns at the plurality of wavelengths such that spatial information of the microscopic objects <NUM> in the interference patterns for each wavelength of the plurality of wavelengths are aligned with each other on the array of light sensitive areas <NUM>. The present arrangement enables combination of the information from each of the plurality of wavelengths into multi-spectral information of the microscopic objects <NUM>.

The device <NUM> further comprises a processor <NUM> configured to perform digital holographic reconstruction on the interference patterns detected by the array of light sensitive areas <NUM>. The digital holographic reconstruction for each of the plurality of wavelengths detected generate a three-dimensional monochromatic image of the microscopic objects <NUM> on the objective slide <NUM>. For the digital holographic reconstruction, the processor <NUM> may utilize any suitable algorithm as known to the person skilled in the art, including a Gerchberg-Saxton algorithm or multi-acquisition (multi-depth and/or multi-wavelength) for phase retrieval, or reconstruction based on angular spectrum diffraction by means of Gabor wavelet transform.

The processor <NUM> is further configured to optionally combine the monochromatic images of the microscopic objects <NUM> generated for each of the wavelengths, to form an aligned multi-spectral image of the microscopic objects <NUM>. Since the spatial information of the microscopic objects <NUM> in the interference patterns for each of the wavelengths are aligned with each other on the array of light sensitive areas <NUM>, combination of the monochromatic images may be performed without further intermediate image transformation such as resampling, rescaling, or dewarping, that may otherwise be required to align the image views of the different wavelengths.

The processor <NUM> may be implemented as a general-purpose processor, which may be provided with instructions, e.g. through computer programs for performing digital holographic reconstruction and for providing any other functionality of the processor <NUM>. Thus, the processor <NUM> may for instance be a central processing unit (CPU).

The processor <NUM> may alternatively be implemented as firmware arranged e.g. in an embedded system, or as a specifically designed processor, such as an Application-Specific Integrated Circuit (ASIC) or a Field-Programmable Gate Array (FPGA).

It should be understood that the signal-to-noise ratio, SNR, of the detected interference patterns may decrease as the distance increases between the microscopic objects <NUM> and the array of light sensitive areas <NUM> of the detector <NUM>. Thus, in order to ensure a good SNR, it may be preferable to have the objective slide <NUM> with the microscopic objects <NUM> arranged at a minimum distance from the array of light sensitive areas <NUM> of the detector <NUM>. Thus, in a practical set-up it would be advantageous to have the objective slide <NUM> arranged in immediate proximity to the detector <NUM>. In this respect, the illustrations in <FIG> as well as in subsequent figures are to be interpreted as schematic illustrations, wherein the objective slide <NUM> is illustrated at a distance away from the detector <NUM> for clear illustrational purposes only.

In the present embodiment, the microscopic objects <NUM> have been placed on a transparent objective slide <NUM> for easy handling of the microscopic objects <NUM> into and out from the device <NUM>. However, it is conceivable that also other solutions for introducing and removing microscopic objects <NUM> to and from the location at which imaging of the microscopic objects <NUM> are performed. By way of example, the microscopic objects <NUM> can be placed on rotating disks, rolls of tape, or in flow channels through which liquid or gaseous flow may pass.

<FIG> illustrates a device <NUM> for imaging microscopic objects <NUM>, according to an embodiment of the inventive concept. The device <NUM> comprises a number of light sources <NUM>0a, <NUM>0b, 210d, each of which is a laser configured to generate light at an individual wavelength or at several different wavelengths in an individual wavelength interval within a wavelength range of at least <NUM>-<NUM>, including wavelengths in the visible and in the SWIR part of the spectrum. For illustrational purposes, the number of light sources 210a, 210b, 210c is illustrated as being three in <FIG>. However, it should be understood that the number of light sources may vary between different embodiments, and therefore may be two or more.

Each of the light sources 210a, 210b, 210c is configured such that the spectral line-width of the generated wavelength does not exceed <NUM>, in order to avoid spectral overlap between the generated wavelengths. It should be realized that other spectral line-widths of the generated wavelengths may be provided, such as spectral line-widths that do not exceed <NUM> or spectral line-widths that do not exceed <NUM>. In particular, when the light source is a laser, a narrow spectral line-width may be provided.

The light from each of the light sources 210a, 210b, 210c is guided by respective optical fibers 212a, 212b, 212c to respective outputs 214a, 214b, 214c. The outputs 214a, 214b, 214c are arranged such that the light exiting the outputs 214a, 214b, 214c is directed towards a microscopic object <NUM>. In this embodiment, an objective slide <NUM> is used on which microscopic objects <NUM> are arranged, and the microscopic objects <NUM> are thereby illuminated on the objective slide <NUM>, at slightly different angles from a first side of the objective slide <NUM>.

At least part of the light is scattered by the microscopic objects <NUM>, whereas non-scattered light passes through the microscopic objects <NUM> and the objective slide <NUM>. The scattered and non-scattered light is transmitted to a detector <NUM> on the opposite side of the objective slide <NUM>, the detector <NUM> comprising an array of light sensitive areas <NUM>. At the detector <NUM>, an interference pattern is formed for each of the wavelengths between light being scattered by the microscopic objects <NUM> and non-scattered light from the light sources 210a, 210b, 210c.

The array of light sensitive areas <NUM> is configured to detect the interference pattern for each wavelength. Each of the light sensitive areas <NUM> are sensitive to detect light spanning the wavelength range of at least <NUM>-<NUM>, thereby being able to detect light in the full range of wavelengths produced by the light sources 210a, 210b, 210c.

The array of light sensitive areas <NUM> may selectably be of either InGaAs type or quantum dot image sensor type or another type that is sensitive to detect light spanning the wavelength range of at least <NUM>-<NUM>, similarly as was described in relation to the embodiment illustrated in <FIG>.

The device <NUM> further comprises a processor <NUM> configured to perform digital holographic reconstruction on the interference patterns detected by the array of light sensitive areas <NUM>, thereby generating a three-dimensional monochromatic image of the microscopic objects <NUM> on the objective slide <NUM>, for each of the wavelengths.

Since the light from the different light sources 210a, 210b, 210c reaches the detector <NUM> with slightly different angles of incidence, the interference patterns may not necessarily be detected such that spatial information of the microscopic objects <NUM> for each of the wavelengths are aligned with each other. The processor <NUM> may therefore be further configured to perform intermediate image transformation such as resampling, rescaling, and/or dewarping. Image transformation may facilitate subsequent optional combination of the monochromatic images of the microscopic objects <NUM>, to form an aligned multispectral image of the microscopic objects <NUM>. Alternatively, it is conceivable that the acquired data be transferred to an external unit such as a computer where processing such as image transformation and/or image combination may be performed.

It should however be realized that, since the same detector <NUM> is used for detecting image information relating to different wavelengths, relatively simple image transformations may be used for forming an aligned multispectral image of the microscopic objects <NUM>.

<FIG> illustrates a device <NUM> for imaging microscopic objects <NUM>, according to an embodiment of the inventive concept. The device <NUM> shares a number of features with device <NUM> illustrated in <FIG>, all of which will not be explicitly repeated in this section. The device <NUM> comprises a number of light sources 310a, 310b, 310c, of the same type as described for device <NUM>.

The light from each of the light sources 310a, 310b, 310c is guided by optical fibers 312a, 312b, 312c, which are combined to a common output <NUM>, from which light from the light sources 310a, 310b, 310c may exit, following a common aligned light path. The output <NUM> is arranged such that the light exiting the output <NUM> illuminates the microscopic objects <NUM> on the objective slide <NUM>.

Scattered and non-scattered light is transmitted to a detector <NUM> comprising an array of light sensitive areas <NUM>, of the same type as described for device <NUM>. The array of light sensitive areas <NUM> is configured to detect the interference pattern for each wavelength.

In the present embodiment, the light from the light sources 310a, 310b, 310c are combined onto a common, aligned light path, prior to exiting the output <NUM>. The illumination therefore follows the same path for each of the wavelengths. Hence, the device <NUM> is configured to detect the interference patterns at the plurality of wavelengths such that spatial information of the microscopic objects <NUM> in the interference patterns for each of the wavelengths are aligned with each other on the array of light sensitive areas <NUM>. The present arrangement facilitates combination of the information from each of the wavelengths into multi-spectral information of the microscopic objects <NUM>.

The processor <NUM> may further be configured to combine the monochromatic images of the microscopic objects <NUM> for each of the wavelengths, to form an aligned multi-spectral image of the microscopic objects <NUM>. Such combination may be performed without further intermediate image transformation, since the spatial information of the microscopic objects <NUM> in the interference patterns for each of the wavelengths are aligned with each other.

<FIG> illustrates a detector <NUM> and an array <NUM> of filters <NUM>, according to an embodiment of the inventive concept. The array <NUM> of filters <NUM> is arranged above the array of light sensitive areas <NUM> such that light passes the array <NUM> of filters <NUM> before reaching the array of light sensitive areas <NUM>. The array <NUM> of filters <NUM> may be arranged close to the array of light sensitive areas <NUM>, such as being arranged directly on the array of light sensitive areas <NUM> or being monolithically integrated with the array of light sensitive areas <NUM>.

The array <NUM> of filters <NUM> comprises a plurality of subsets 442a, 442b, 442c of filters <NUM>, each of the filters <NUM> in the subset 442a, 442b, 442c of filters <NUM> being arranged in front of a light sensitive area <NUM>. Each of the subsets 442a, 442b, 442c of filters <NUM> is configured to transmit light at one of the plurality of wavelengths, so that each of the subsets 442a, 442b, 442c of filters <NUM> transmit light at a different wavelength than other subsets 442a, 442b, 442c. By the present arrangement, each of the wavelengths of the plurality of wavelengths is transmitted through a corresponding subset 442a, 442b, 442c of filters <NUM>, such that some of the light sensitive areas <NUM> detect the interference pattern for light at a first wavelength, some other light sensitive areas <NUM> detect the interference pattern for light as a second wavelength, and so on. In the manner described above, a detector assembly may be provided with which light at different wavelengths may be detected by different light sensitive areas <NUM>, thereby allowing simultaneous detection of the individual interference patterns by the same detector <NUM>.

Each of the embodiments illustrated in <FIG>, <FIG> may optionally be provided with the present array <NUM> of filters <NUM>, thereby enabling simultaneous detection of the plurality of wavelengths.

<FIG> illustrates a schematic block diagram shortly summarizing the method for imaging a microscopic object, as previously described in relation to the operation of the devices <NUM>, <NUM>, <NUM>. It should be understood that the steps of the method, although listed in a specific order herein, may be performed in any order suitable.

The method may comprise generating S502 light at a plurality of wavelengths within a wavelength range of at least <NUM>-<NUM>, by at least one light source, the plurality of wavelengths comprising at least one wavelength in a visible part of the wavelength range and at least one wavelength in a short-wave infrared, SWIR, part of the wavelength range.

The method may further comprise illuminating S504 the microscopic object with the generated light such that at least part of the light is scattered by the microscopic object, forming scattered light. It should be understood that there may be implementations of the methods in which the illuminating S504 the microscopic objects may be performed sequentially by the plurality of wavelengths. It should be understood that there may be implementations of the methods in which the illuminating S504 the microscopic objects may be performed simultaneously by the plurality of wavelengths.

The method may further comprise transmitting S506 the scattered light and non-scattered light, from the same light source, to an array of light sensitive areas, such that an interference pattern is formed by interference between the scattered light and the non-scattered light.

The method may further comprise detecting S508, by the array of light sensitive areas sensitive to detect light spanning the wavelength range of at least <NUM>-<NUM>, interference patterns at each of the plurality of wavelengths. It should be understood that there may be implementations of the methods in which the detecting S508 interference patterns may be performed sequentially at the plurality of wavelengths. It should be understood that there may be implementations of the methods in which the detecting S508 interference patterns may be performed simultaneously at the plurality of wavelengths.

In the above the inventive concept has mainly been described with reference to a limited number of examples. However, as is readily appreciated by a person skilled in the art, other examples than the ones disclosed above are equally possible within the scope of the inventive concept, as defined by the appended claims.

Claim 1:
A device (<NUM>, <NUM>, <NUM>) for imaging of a microscopic object (<NUM>), the device (<NUM>, <NUM>, <NUM>) comprising:
an array of light sensitive areas (<NUM>, <NUM>, <NUM>, <NUM>), each of the light sensitive areas (<NUM>, <NUM>, <NUM>, <NUM>) being sensitive to detect light spanning a wavelength range of at least <NUM>-<NUM>;
at least one light source (<NUM>, <NUM>, <NUM>) configured to generate light at a plurality of wavelengths within the wavelength range, the plurality of wavelengths comprising at least one wavelength in a visible part of the wavelength range and at least one wavelength in a short-wave infrared, SWIR, part of the wavelength range, and arranged to illuminate the microscopic object (<NUM>) with the generated light such that at least part of the light is scattered by the microscopic object (<NUM>), forming scattered light;
wherein the device (<NUM>, <NUM>, <NUM>) is configured to transmit the scattered light and non-scattered light, from the same light source (<NUM>, <NUM>, <NUM>), to the array of light sensitive areas (<NUM>, <NUM>, <NUM>, <NUM>), wherein the array of light sensitive areas (<NUM>, <NUM>, <NUM>, <NUM>) is configured to detect an interference pattern formed by interference between the scattered light and the non-scattered light, for each wavelength in the plurality of wavelengths.