Patent Description:
The present disclosure relates to a class of multi-targeted inhibitors of IRAK4 and BTK, and a use thereof in the preparation of a medicament for treating IRAK4- and BTK-related diseases. The present disclosure specifically relates to a compound represented by formula (II), an isomer thereof or a pharmaceutically acceptable salt thereof.

Interleukin-<NUM> receptor-associated kinase <NUM> (IRAK4) is a serine/threonine-specific protein kinase, a member of the tyrosine like kinase (TLK) family, and a key node in the innate immune response involving interleukin-<NUM>, <NUM>, <NUM> receptors and Toll-like receptors. After binding with interleukin receptor or Toll-like receptor, extracellular signal molecules recruit to form MyD88: IRAK4: IRAK1/<NUM> multiprotein complex, leading to phosphorylation of IRAK1/<NUM> and mediating a series of downstream signal transduction, thus activating p38, JNK and NF-κ B signaling pathways, and finally leading to the expression of proinflammatory cytokines. Clinicopathological studies have shown that individuals with IRAK4 mutation have a protective effect on chronic lung disease and inflammatory bowel disease. IRAK4 deficiency itself is non-lethal, individuals can survive to adulthood, and the risk of infection decreases with age. Therefore, IRAK4 has become an important therapeutic target, which can be widely used in the treatment of inflammatory diseases, immune diseases, tumor diseases and other diseases. As shown in the following figure, BAY-<NUM> and BAY-<NUM> are small molecule IRAK4 inhibitors developed by Bayer Company, at present, clinical research on immune and tumor diseases has been carried out.

Activated B-cell-like diffuse large B-cell lymphoma (ABC-DLBCL) is a highly invasive and poorly prognostic DLBCL, which is usually characterized by abnormalities of B-cell receptor (BCR) pathway and myeloid-like differentiation factor <NUM> (MyD88) pathway, which further leads to the continuous activation of nuclear factor κ B protein (NF-κ B) signaling pathway. CD79 mutation is a common abnormal mutation in BCR pathway, and BTK inhibitors such as Ibrutinib can inhibit the abnormal activation of NF-κ B signaling pathway caused by CD79 mutation, thus inhibiting the proliferation of ABC-DLBCL cells. The abnormal MyD88 pathway is mainly MyD88L265P point mutation, which accounts for about <NUM>%, IRAK4 inhibitors can effectively block the abnormally activated MyD88 signaling pathway and further block the abnormal activation of the NP-κB pathway. However, ABC-DLBCL patients with MyD88L265P mutations have a poor response to BCR inhibitors due to abnormal MyD88 signaling pathway, and a large number of research data from Bayer, Nimbus and AstraZeneca indicate that the combination of IRAK4 inhibitor and BTK inhibitor can significantly improve the in vivo efficacy of Ibrutinib in ABC-DLBCL xenotransplantation animal model. If the abnormality of BCR pathway and MyD88 pathway can be effectively inhibited at the same time, it will be a more effective way to treat ABC-DLBCL, therefore, developing RAK4 and BTK dual-target inhibitors can obtain double benefits in blocking NF-κ B pathway, which is a very efficient and effective strategy in terms of therapeutic mechanism and provides a potentially effective new therapeutic method for ABC-DLBCL patients.

<CIT>) discloses IRAK4 inhibitors, specifically discloses compounds represented by formula (I) therein or pharmaceutically acceptable salts thereof; <CIT>) discloses IRAK4 inhibitors, specifically discloses compounds represented by formula (I) or (II) therein, or pharmaceutically acceptable salts thereof; <CIT>) discloses IRAK4 inhibitors, specifically discloses compounds represented by formula (I) therein or pharmaceutically acceptable salts thereof; <NPL>) (publication date: May, <NUM>) discloses IRAK4 inhibitors for inflammation.

Content of the present inventionThe present invention is defined by the appended claims. Especially, the present invention provides a compound of formula (II), or a pharmaceutically acceptable salt thereof, as defined in claim <NUM>, a pharmaceutical composition as defined in claim <NUM> and a compound for use in the treatment of diseases related to IRAK4 and BTK as defined claim <NUM>.

The present disclosure provides a compound represented by formula (II), an isomer thererof or a pharmaceutically acceptable salt thereof,
<CHM>
wherein,.

In some embodiments of the present disclosure, the R<NUM> is selected from H, F, Cl, Br, I, OH, NH<NUM>, CN, C<NUM>-<NUM> alkyl, cyclopropyl and -C(=O)-NH<NUM>, wherein the C<NUM>-<NUM> alkyl, cyclopropyl and -C(=O)-NH<NUM> are optionally substituted by <NUM>, <NUM> or <NUM> Ra, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from H, F, Cl, Br, I, OH, NH<NUM>, CN, C<NUM>-<NUM> alkyl and cyclopropyl, wherein the C<NUM>-<NUM> alkyl and cyclopropyl are optionally substituted by <NUM>, <NUM> or <NUM> Ra, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from CN, CH<NUM>, CF<NUM>,
<CHM>
and -C(=O)-NH<NUM>, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from CN, CH<NUM>, CF<NUM>,
<CHM>
and
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the Rb is each independently selected from H, D, F, Cl, Br, I, OH, NH<NUM>, CN, CH<NUM>, CH<NUM>CH<NUM>, CH<NUM>CH<NUM>CH<NUM>, CH(CH<NUM>)<NUM>, COOH,
<CHM>
wherein the OH, NH<NUM>, CH<NUM>, CH<NUM>CH<NUM>, CH<NUM>CH<NUM>CH<NUM>, CH(CH<NUM>)<NUM>,
<CHM>
are optionally substituted by <NUM>, <NUM> or <NUM> R, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the Rb is each independently selected from H, D, F, Cl, OH, OCH<NUM>, CN, CH<NUM>, CH<NUM>OH, CH<NUM>NH<NUM>-, COOH,
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from thienyl, phenyl, pyridyl, cyclopropyl, cyclohexyl
<CHM>
wherein the thienyl, phenyl, pyridyl, cyclopropyl, cyclohexyl,
<CHM>
are optionally substituted by <NUM>, <NUM>, <NUM>, <NUM> or <NUM> Rb, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from
<CHM>
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from C<NUM>-<NUM> alkyl, wherein the C<NUM>-<NUM> alkyl is optionally substituted by <NUM>, <NUM> or <NUM> Rc, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from CH<NUM>CH<NUM>, CH<NUM>CH<NUM>CH<NUM>, CH(CH<NUM>)<NUM>, CH<NUM>CH<NUM>CH<NUM>CH<NUM>, CH<NUM>CH(CH<NUM>)<NUM> and CH<NUM>CH<NUM>CH(CH<NUM>)<NUM>, wherein the CH<NUM>CH<NUM>, CH<NUM>CH<NUM>CH<NUM>, CH(CH<NUM>)<NUM>, CH<NUM>CH<NUM>CH<NUM>CH<NUM>, CH<NUM>CH(CH<NUM>)<NUM> and CH<NUM>CH<NUM>CH(CH<NUM>)<NUM> are optionally substituted by <NUM>, <NUM> or <NUM> Rc, and the other variables are as defined in the present disclosure.

The present disclosure also provides a compound represented by formula (II), an isomer thererof or a pharmaceutically acceptable salt thereof,
<CHM>
wherein,.

In some embodiments of the present disclosure, the Rb is independently each selected from H, F, Cl, Br, I, OH, NH<NUM>, CN, CH<NUM>,
<CHM>
wherein the CH<NUM>,
<CHM>
are optionally substituted by R, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the Rb is each independently selected from H, F, OH, CN, CH<NUM>, CH<NUM>OH, CH<NUM>NH<NUM>,
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from phenyl, pyridyl, cyclopropyl, cyclohexyl,
<CHM>
wherein the phenyl, pyridyl, cyclopropyl, cyclohexyl,
<CHM>
are optionally substituted by <NUM>, <NUM> or <NUM> Rb, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from
<CHM>
<CHM>
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from
<CHM>
and the other variables are as defined in the present disclosure.

The present disclosure also provides a compound represented by formula (I), an isomer thererof or a pharmaceutically acceptable salt thereof,
<CHM>
wherein,.

In some embodiments of the present disclosure, the R<NUM> is selected from H, F, Cl, Br, I, OH, NH<NUM>, CN and C<NUM>-<NUM> alkyl, wherein the C<NUM>-<NUM> alkyl is optionally substituted by <NUM>, <NUM> or <NUM> Ra, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is CH<NUM>, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the Rb is selected from H, F, Cl, Br, I, OH, NH<NUM>, CN, CH<NUM>,
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from C<NUM>-<NUM> cycloalkyl and <NUM>-<NUM> membered heterocycloalkyl, wherein the C<NUM>-<NUM> cycloalkyl and <NUM>-<NUM> membered heterocycloalkyl are optionally substituted by <NUM>, <NUM> or <NUM> Rb, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the R<NUM> is selected from morpholinyl, piperidinyl, piperazinyl, tetrahydropyranyl and cyclopropyl, wherein the morpholinyl, piperidinyl, piperazinyl, tetrahydropyranyl and cyclopropyl are optionally substituted by <NUM>, <NUM> or <NUM> Rb, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the L<NUM> is selected from C<NUM>-<NUM> alkyl, wherein the C<NUM>-<NUM> alkyl is optionally substituted by <NUM>, <NUM> or <NUM> Rc, and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the L<NUM> is
<CHM>
and the other variables are as defined in the present disclosure.

In some embodiments of the present disclosure, the compound, the isomer or the pharmaceutically acceptable salt thereof, and the compound is selected from:
<CHM>
<CHM>
<CHM>
and
<CHM>
wherein, L<NUM> is selected from C<NUM>-<NUM> alkyl, and R<NUM>, R<NUM> and Rb are as defined in the present disclosure.

There are also some embodiments of the present disclosure obtained by an arbitrary combination of the above variables.

The present disclosure also provides a compound represented by the following formula, an isomer thereor or a pharmaceutically acceptable salt thereof,
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
<CHM>
and
<CHM>.

The present disclosure also provides a pharmaceutical composition, comprising a therapeutically effective amount of the compound described above, the isomer thereof or the pharmaceutically acceptable salt thereof as an active ingredient and a pharmaceutically acceptable carrier.

The present disclosure also provides the compound described above, the isomer thereof or the pharmaceutically acceptable salt thereof, or the pharmaceutical composition described above for use in the treatment of diseases related to IRAK4 and BTK.

The compound of the present disclosure generally exhibits good inhibitory activity against IRAK4 and BTK. The compound of the present disclosure generally exhibits a good activity of inhibiting cell TNF-α production in THP-<NUM> cells, a good activity of inhibiting cell proliferation in OCI-LY10, OCI-LY3 and TMD-<NUM> cells, and a good in vivo efficacy in subcutaneous xenograft tumor model of human B-cell lymphoma OCI-LY10 cells.

Unless otherwise specified, the following terms and phrases when used herein have the following meanings. A specific term or phrase should not be considered indefinite or unclear in the absence of a particular definition, but should be understood in the ordinary sense. When a trading name appears herein, it is intended to refer to its corresponding commodity or active ingredient thereof.

The term "pharmaceutically acceptable" is used herein in terms of those compounds, materials, compositions, and/or dosage forms, which are suitable for use in contact with human and animal tissues within the scope of reliable medical judgment, with no excessive toxicity, irritation, an allergic reaction or other problems or complications, commensurate with a reasonable benefit/risk ratio.

The term "pharmaceutically acceptable salt" refers to a salt of the compound of the present disclosure that is prepared by reacting the compound having a specific substituent of the present disclosure with a relatively non-toxic acid or base. When the compound of the present disclosure contains a relatively acidic functional group, a base addition salt can be obtained by bringing the neutral form of the compound into contact with a sufficient amount of base in a pure solution or a suitable inert solvent. The pharmaceutically acceptable base addition salt includes a salt of sodium, potassium, calcium, ammonium, organic amine or magnesium, or similar salts. When the compound of the present disclosure contains a relatively basic functional group, an acid addition salt can be obtained by bringing the neutral form of the compound into contact with a sufficient amount of acid in a pure solution or a suitable inert solvent. Examples of the pharmaceutically acceptable acid addition salt include an inorganic acid salt, wherein the inorganic acid includes, for example, hydrochloric acid, hydrobromic acid, nitric acid, carbonic acid, bicarbonate, phosphoric acid, monohydrogen phosphate, dihydrogen phosphate, sulfuric acid, hydrogen sulfate, hydroiodic acid, and phosphorous acid; and an organic acid salt, wherein the organic acid includes, for example, acetic acid, propionic acid, isobutyric acid, maleic acid, malonic acid, benzoic acid, succinic acid, suberic acid, fumaric acid, lactic acid, mandelic acid, phthalic acid, benzenesulfonic acid, p-toluenesulfonic acid, citric acid, tartaric acid, and methanesulfonic acid; and salts of amino acid (such as arginine), and a salt of an organic acid such as glucuronic acid. Certain specific compounds of the present disclosure contain both basic and acidic functional groups, thus can be converted to any base or acid addition salt.

The pharmaceutically acceptable salt of the present disclosure can be prepared from the parent compound that contains an acidic or basic moiety by conventional chemical method. Generally, such salt can be prepared by reacting the free acid or base form of the compound with a stoichiometric amount of an appropriate base or acid in water or an organic solvent or a mixture thereof.

The compounds of the present disclosure may exist in specific geometric or stereoisomeric forms. The present disclosure contemplates all such compounds, including cis and trans isomers, (-)-and (+)-enantiomers, (R)- and (S)-enantiomers, diastereomers isomers, (D)-isomers, (L)-isomers, and racemic and other mixtures thereof, such as enantiomers or diastereomeric enriched mixtures, all of which are within the scope of the present disclosure. Additional asymmetric carbon atoms may be present in substituents such as alkyl. All these isomers and their mixtures are included within the scope of the present disclosure.

Unless otherwise specified, D in the present disclosure represents deuterium (<NUM>H).

Unless otherwise specified, the term "enantiomer" or "optical isomer" refers to stereoisomers that are mirror images of each other.

Unless otherwise specified, the term "cis-trans isomer" or "geometric isomer" is caused by the inability to rotate freely of double bonds or single bonds of ring-forming carbon atoms.

Unless otherwise specified, the term "diastereomer" refers to a stereoisomer in which a molecule has two or more chiral centers and the relationship between the molecules is not mirror images.

Unless otherwise specified, "(+)" refers to dextrorotation, "(-)" refers to levorotation, and or "(±)" refers to racemic.

Unless otherwise specified, the absolute configuration of a stereogenic center is represented by a wedged solid bond (<IMG>) and a wedged dashed bond (<IMG>), and the relative configuration of a stereogenic center is represented by a straight solid bond (<IMG>) and a straight dashed bond (<IMG>), a wave line (<IMG>) is used to represent a wedged dashed bond (<IMG>) or a wedged dashed bond (<IMG>), or the wave line (<IMG>) is used to represent a straight solid bond (<IMG>) and a straight dashed bond (<IMG>).

Unless otherwise specified, when double bond structure, such as carbon-carbon double bond, carbon-nitrogen double bond, and nitrogen-nitrogen double bond, exists in the compound, and each of the atoms on the double bond is connected to two different substituents (including the condition where a double bond contains a nitrogen atom, the lone pair of electrons attached on the nitrogen atom is regarded as a substituent connected), if the atom on the double bond in the compound is connected to its substituent by a wave line (<IMG>), this refers to the (Z) isomer, (E) isomer or a mixture of two isomers of the compound. For example, the following formula (A) means that the compound exists as a single isomer of formula (A-<NUM>) or formula (A-<NUM>) or as a mixture of two isomers of formula (A-<NUM>) and formula (A-<NUM>); the following formula (B) means that the compound exists in the form of a single isomer of formula (B-<NUM>) or formula (B-<NUM>) or in the form of a mixture of two isomers of formula (B-<NUM>) and formula (B-<NUM>). The following formula (C) means that the compound exists as a single isomer of formula (C-<NUM>) or formula (C-<NUM>) or as two a mixture of two isomers of formula (C-<NUM>) and formula (C-<NUM>). <CHM>
<CHM>
<CHM>.

Unless otherwise specified, the term "tautomer" or "tautomeric form" means that at room temperature, the isomers of different functional groups are in dynamic equilibrium and can be transformed into each other quickly. If tautomers possibly exist (such as in solution), the chemical equilibrium of tautomers can be reached. For example, proton tautomer (also called prototropic tautomer) includes interconversion through proton migration, such as keto-enol isomerization and imine-enamine isomerization. Valence tautomer includes some recombination of bonding electrons for mutual transformation. A specific example of keto-enol tautomerization is the tautomerism between two tautomers of pentane-<NUM>, <NUM>-dione and <NUM>-hydroxypent-<NUM>-en-<NUM>-one.

Unless otherwise specified, the terms "enriched in one isomer", "enriched in isomers", "enriched in one enantiomer" or "enriched in enantiomers" refer to the content of one of the isomers or enantiomers is less than <NUM>%, and the content of the isomer or enantiomer is greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%, or greater than or equal to <NUM>%.

Unless otherwise specified, the term "isomer excess" or "enantiomeric excess" refers to the difference between the relative percentages of two isomers or two enantiomers. For example, if the content of one isomer or enantiomer is <NUM>%, and the content of the other isomer or enantiomer is <NUM>%, the isomer or enantiomer excess (ee value) is <NUM>%.

Optically active (R)- and (S)-isomer, or D and L isomer can be prepared using chiral synthesis or chiral reagents or other conventional techniques. If one kind of enantiomer of certain compound of the present disclosure is to be obtained, the pure desired enantiomer can be obtained by asymmetric synthesis or derivative action of chiral auxiliary followed by separating the resulting diastereomeric mixture and cleaving the auxiliary group. Alternatively, when the molecule contains a basic functional group (such as amino) or an acidic functional group (such as carboxyl), the compound reacts with an appropriate optically active acid or base to form a salt of the diastereomeric isomer which is then subjected to diastereomeric resolution through the conventional method in the art to obtain the pure enantiomer. In addition, the enantiomer and the diastereoisomer are generally isolated through chromatography which uses a chiral stationary phase and optionally combines with a chemical derivative method (such as carbamate generated from amine).

The compound of the present disclosure may contain an unnatural proportion of atomic isotope at one or more than one atom(s) that constitute the compound. For example, the compound can be radiolabeled with a radioactive isotope, such as tritium (<NUM>H), iodine-<NUM> (<NUM>I) or C-<NUM> (<NUM>C). For another example, deuterated drugs can be formed by replacing hydrogen with heavy hydrogen, and the bond formed by deuterium and carbon is stronger than that of ordinary hydrogen and carbon; compared with non-deuterated drugs, deuterated drugs have the advantages of reduced toxic and side effects, increased drug stability, enhanced efficacy, extended biological half-life of drugs, etc. All isotopic variations of the compound of the present disclosure, whether radioactive or not, are encompassed within the scope of the present disclosure.

The term "optional" or "optionally" means that the subsequent event or condition may occur but not requisite, that the term includes the instance in which the event or condition occurs and the instance in which the event or condition does not occur.

The term "substituted" means one or more than one hydrogen atom (s) on a specific atom are substituted with the substituent, including deuterium and hydrogen variables, as long as the valence of the specific atom is normal and the substituted compound is stable. When the substituent is an oxygen (i.e., =O), it means two hydrogen atoms are substituted. Positions on an aromatic ring cannot be substituted with a ketone. The term "optionally substituted" means an atom can be substituted with a substituent or not, unless otherwise specified, the type and number of the substituent may be arbitrary as long as being chemically achievable.

When any variable (such as R) occurs in the constitution or structure of the compound more than once, the definition of the variable at each occurrence is independent. Thus, for example, if a group is substituted with <NUM>-<NUM> R, the group can be optionally substituted with up to two R, wherein the definition of R at each occurrence is independent. Moreover, a combination of the substituent and/or the variant thereof is allowed only when the combination results in a stable compound.

When the number of a linking group is <NUM>, such as -(CRR)<NUM>-, it means that the linking group is a single bond.

When a substituent is vacant, it means that the substituent does not exist, for example, when X is vacant in A-X, the structure of A-X is actually A. When the enumerative substituent does not indicate by which atom it is linked to the group to be substituted, such substituent can be bonded by any atom thereof. For example, when pyridyl acts as a substituent, it can be linked to the group to be substituted by any carbon atom on the pyridine ring.

When the enumerative linking group does not indicate the direction for linking, the direction for linking is arbitrary, for example, the linking group L contained in
<CHM>
is -M-W-, then -M-W- can link ring A and ring B to form
<CHM>
in the direction same as left-to-right reading order, and form
<CHM>
in the direction contrary to left-to-right reading order. A combination of the linking groups, substituents and/or variables thereof is allowed only when such combination can result in a stable compound.

Unless otherwise specified, when a group has one or more linkable sites, any one or more sites of the group can be linked to other groups through chemical bonds. When the linking site of the chemical bond is not positioned, and there is H atom at the linkable site, then the number of H atom at the site will decrease correspondingly with the number of chemical bond linking thereto so as to meet the corresponding valence. The chemical bond between the site and other groups can be represented by a straight solid bond (<IMG>), a straight dashed bond (<IMG>) or a wavy line
<CHM>
For example, the straight solid bond in -OCH<NUM> means that it is linked to other groups through the oxygen atom in the group; the straight dashed bonds in
<CHM>
means that it is linked to other groups through the two ends of nitrogen atom in the group; the wave lines in
<CHM>
means that the phenyl group is linked to other groups through carbon atoms at position <NUM> and position <NUM>;
<CHM>
means that it can be linked to other groups through any linkable sites on the piperidinyl by one chemical bond, including at least four types of linkage, including
<CHM>
Even though the H atom is drawn on the -N-,
<CHM>
still includes the linkage of
<CHM>
merely when one chemical bond was connected, the H of this site will be reduced by one to the corresponding monovalent piperidinyl.

Unless otherwise specified, the term "C<NUM>-<NUM> alkyl" refers to a linear or branched saturated hydrocarbon group consisting of <NUM> to <NUM> carbon atoms. The C<NUM>-<NUM> alkyl includes C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM> and C<NUM> alkyl. It can be monovalent (such as methyl), divalent (such as methylene) or multivalent (such as methine). Examples of C<NUM>-<NUM> alkyl include but are not limited to methyl (Me), ethyl (Et), propyl (including n-propyl and isopropyl), butyl (including n-butyl, isobutyl, s-butyl, and t-butyl), pentyl (including n-pentyl, isopentyl and neopentyl), and hexyl.

Unless otherwise specified, the term "C<NUM>-<NUM> alkyl" refers to a linear or branched saturated hydrocarbon group consisting of <NUM> to <NUM> carbon atoms. The C<NUM>-<NUM> alkyl includes C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>, C<NUM>, C<NUM> and C<NUM> alkyl. Examples of C<NUM>-<NUM> alkyl include but are not limited to ethyl (Et), propyl (including n-propyl and isopropyl), butyl (including n-butyl, isobutyl, s-butyl, and t-butyl), and pentyl (including n-pentyl, isopentyl and neopentyl).

Unless otherwise specified, the term "C<NUM>-<NUM> alkyl" refers to a linear or branched saturated hydrocarbon group consisting of <NUM> to <NUM> carbon atoms. The C<NUM>-<NUM> alkyl group includes C<NUM>-<NUM> and C<NUM>-<NUM> alkyl; it can be monovalent (such as methyl), divalent (such as methylene) or multivalent (such as methine). Examples of C<NUM>-<NUM> alkyl include but are not limited to methyl (Me), ethyl (Et), propyl (including n-propyl and isopropyl), etc..

Unless otherwise specified, the term "C<NUM>-<NUM> alkoxy" refers to an alkyl containing <NUM> to <NUM> carbon atoms that are connected to the rest of the molecule through an oxygen atom. The C<NUM>-<NUM> alkoxy includes C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>, C<NUM> and C<NUM> alkoxy. Examples of C<NUM>-<NUM> alkoxy include, but are not limited to, methoxy, ethoxy, propoxy (including n-propoxy and isopropoxy), etc..

Unless otherwise specified, "C<NUM>-<NUM> cycloalkyl" refers to a saturated cyclic hydrocarbon group consisting of <NUM> to <NUM> carbon atoms, including monocyclic and bicyclic systems, wherein the bicyclic systems include spiro ring, fused ring and bridged ring. The C<NUM>-<NUM> cycloalkyl includes C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM>, C<NUM>-<NUM> or C<NUM>-<NUM> cycloalkyl, the C<NUM>-<NUM> cycloalkyl can be monovalent, divalent or multivalent. Examples of C<NUM>-<NUM> cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, norbornyl, and [<NUM>. <NUM>] dicyclooctyl.

Unless otherwise specified, the term "<NUM>-<NUM> membered heterocycloalkyl" by itself or in combination with other terms refers to a saturated cyclic group consisting of <NUM> to <NUM> ring atoms, wherein <NUM>, <NUM>, <NUM> or <NUM> ring atoms are heteroatoms independently selected from O, S and N, and the rest are carbon atoms, wherein nitrogen atoms are optionally quaternized, and nitrogen and sulfur heteroatoms can be optionally oxidized (i.e., NO and S(O)p, p is <NUM> or <NUM>). The <NUM>-<NUM> membered heterocycloalkyl includes monocyclic and bicyclic systems, wherein the bicyclic systems include spiro ring, fused ring and bridged ring. In addition, with regard to the "<NUM>-<NUM> membered heterocycloalkyl", a heteroatom may occupy the connection position of the heterocycloalkyl with the rest of the molecule. The <NUM>-<NUM> membered heterocycloalkyl includes <NUM>-<NUM> membered, <NUM>-<NUM> membered, <NUM>-<NUM> membered, <NUM>-<NUM> membered, <NUM>-membered, <NUM>-membered, and <NUM>-membered heterocycloalkyl. Examples of <NUM>-<NUM> membered heterocycloalkyl include, but are not limited to, azetidinyl, oxetanyl, thietanyl, pyrrolidinyl, pyrazolidinyl, imidazolidinyl, tetrahydrothienyl (including tetrahydrothiophen-<NUM>-yl and tetrahydrothiophen-<NUM>-yl), tetrahydrofuranyl (including tetrahydrofuran-<NUM>-yl), tetrahydropyranyl, piperidinyl (including <NUM>-piperidinyl, <NUM>-piperidinyl and <NUM>-piperidinyl), piperazinyl (including <NUM>-piperazinyl and <NUM>-piperazinyl), morpholinyl (including <NUM>-morpholinyl and <NUM>-morpholinyl), dioxinyl, dithianyl, isoxazolidinyl, isothiazolidinyl, <NUM>,<NUM>-oxazinyl, <NUM>,<NUM>-thiazinyl, hexahydropyridazinyl, homopiperazinyl, homopiperidinyl or dioxacycloheptyl.

The term "leaving group" refers to a functional group or atom which can be replaced by another functional group or atom through a substitution reaction (such as affinity substitution reaction). For example, representative leaving groups include triflate; chlorine, bromine, and iodine; sulfonate group, such as mesylate, tosylate, p-bromobenzenesulfonate and p-toluenesulfonates; acyloxy, such as acetoxy and trifluoroacetoxy.

The term "protecting group" includes, but is not limited to "amino protecting group", "hydroxy protecting group" or "thio protecting group". The term "amino protecting group" refers to a protecting group suitable for blocking the side reaction on the nitrogen of an amino. Representative amino protecting groups include, but are not limited to: formyl; acyl, such as alkanoyl (e.g., acetyl, trichloroacetyl or trifluoroacetyl); alkoxycarbonyl, such as tert-butoxycarbonyl (Boc); arylmethoxycarbonyl such as benzyloxycarbonyl (Cbz) and <NUM>-fluorenylmethoxycarbonyl (Fmoc); arylmethyl, such as benzyl (Bn), trityl (Tr), <NUM>,<NUM>-bis-(<NUM>'-methoxyphenyl)methyl; silyl, such as trimethylsilyl (TMS) and tert-butyldimethylsilyl (TBS). The term "hydroxy protecting group" refers to a protecting group suitable for blocking the side reaction on hydroxy. Representative hydroxy protecting groups include, but are not limited to: alkyl, such as methyl, ethyl, and tert-butyl; acyl, such as chain alkanoyl (e.g., acetyl); arylmethyl, such as benzyl (Bn), p-methoxybenzyl (PMB), <NUM>-fluorenylmethyl (Fm), and diphenylmethyl (benzhydryl, DPM); silyl, such as trimethylsilyl (TMS) and tert-butyl dimethyl silyl (TBS).

The compounds of the present disclosure can be prepared by a variety of synthetic methods known to those skilled in the art, including the specific embodiments listed below, the embodiments formed by their combination with other chemical synthesis methods, and equivalent alternatives known to those skilled in the art, preferred implementations include but are not limited to the embodiments of the present disclosure.

The solvent used in the present disclosure is commercially available. The following abbreviations are used in the present disclosure: DMSO refers to dimethyl sulfoxide; EtOH refers to ethanol; MeOH refers to methanol; M refers to mol/L; N/A refers to not tested; MgCl<NUM> refers to magnesium chloride; EGTA refers to ethylenebis(oxyethylenenitrilo)tetraacetic acid; and Na<NUM>VO<NUM> refers to sodium vanadate.

The compounds of the present disclosure are named according to the conventional naming principles in the art or by ChemDraw® software, and the commercially available compounds use the supplier catalog names.

The following embodiment further illustrates the present disclosure. The present disclosure has been described in detail herein, and its specific embodiments have also been disclosed; for one skilled in the art, it is obvious to make various modifications and improvements to the embodiments of the present disclosure without departing from the scope of the present disclosure.

Ethyl succinyl chloride (<NUM>) was added to acetonitrile (<NUM>) and the mixture was stirred evenly; (trimethylsilyl)diazomethane (<NUM>, <NUM>) was added dropwise to the reaction system and the mixture was stirred at <NUM> for <NUM> hours. Then, hydrobromic acid acetic acid solution (<NUM>, <NUM>% content) was added dropwise to the reaction system at <NUM>, and the mixture was stirred at <NUM> for <NUM> hours. The reaction mixture was concentrated under reduced pressure to remove acetonitrile; the residue was poured into ethyl acetate (<NUM>), and washed with saturated sodium bicarbonate aqueous solution (<NUM>×<NUM>). The organic phase was separated and dried over an appropriate amount of anhydrous sodium sulfate. The desiccant was removed by filtration, and the filtrate was concentrated to dryness under reduced pressure to obtain a crude product. The crude product was purified by column chromatography (eluent: petroleum ether-petroleum ether: ethyl acetate = <NUM>:<NUM>) to obtain compound A1.

Palladium acetate (<NUM>), cesium carbonate (<NUM>) and tri-o-tolylphosphine (<NUM>) were added to a mixed solution of <NUM>-bromo-<NUM>-methylpyridine (<NUM>), ethyl oxazole-<NUM>-carboxylate (<NUM>) and N,N-dimethylformamide (<NUM>). The mixture was replaced with nitrogen three times, and stirred at <NUM> for <NUM> hours. Then the reaction mixture was cooled to room temperature, and filtered by celite. The filtrate was concentrated to dryness under reduced pressure to obtain a crude product. The crude product was purified by column chromatography (eluent: petroleum ether: ethyl acetate = <NUM>:<NUM>-<NUM>:<NUM>) to obtain compound A2-<NUM>.

Compound A2-<NUM> (<NUM>) was dissolved in methanol (<NUM>) and water (<NUM>) and the mixture was stirred evenly; sodium hydroxide (<NUM>) was added to the reaction system and the mixture was stirred at <NUM> for <NUM> hours. Methanol was removed by concentration under reduced pressure, and the aqueous phase was extracted with tert-butyl methyl ether (<NUM> × <NUM>). The aqueous phase was separated, and the pH value was adjusted to <NUM> with <NUM> hydrochloric acid. The aqueous phase was concentrated to dryness under reduced pressure, and toluene (<NUM>) was added to the residue and stirred evenly. The mixture was filtered, and the filtrate was concentrated to dryness under reduced pressure to obtain compound A2. LCMS (ESI) m/z = <NUM>[M+H]+. <NUM>H NMR (<NUM>, MeOH-d<NUM>) δ = <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>).

Each fragment compound in Table <NUM> below was synthesized with reference to the synthesis steps of compound A2.

Each intermediate in Table <NUM> below was a commercially available reagent.

<NUM>-Chloro-<NUM>-nitro-pyridin-<NUM>-amine (<NUM>) was dissolved in tetrahydrofuran (<NUM>) and then piperidine (<NUM>) was added. The mixture was stirred at <NUM> for <NUM> hours, the reaction mixture was concentrated to dryness under reduced pressure; ethyl acetate (<NUM>) was added to the residue and the mixture was slurried. Then the mixture was filtered, and the filtrate was collected. The filtrate was concentrated to dryness under reduced pressure to obtain a crude product, then the crude product was purified by column chromatography (petroleum ether: ethyl acetate = <NUM>:<NUM>-<NUM>:<NUM>) to obtain compound WX001-<NUM>.

A mixture of compound WX001-<NUM> (<NUM>) and intermediate A1 (<NUM>) was replaced with nitrogen three times, and then stirred at <NUM> for <NUM> hours. The reaction mixture was cooled to room temperature, then poured into water (<NUM>), and dichloromethane (<NUM>×<NUM>) was added for extraction. The organic phases were combined and dried over an appropriate amount of anhydrous sodium sulfate. The desiccant was removed by filtration, and the filtrate was concentrated to dryness under reduced pressure to obtain a crude product. The crude product was purified by column chromatography (eluent: dichloromethane: methanol = <NUM>:<NUM>- <NUM>:<NUM>) to obtain compound WX001-<NUM>.

Raney nickel (<NUM>) was added to a solution of WX001-<NUM> (<NUM>) in EtOH (<NUM>) and the mixture was stirred under H<NUM> (<NUM> Psi) at <NUM> for <NUM> hour. The mixture was filtered to remove the catalyst, and the filtrate was concentrated to dryness under reduced pressure to obtain compound WX001-<NUM>.

Compound WX001-<NUM> (<NUM>), A2 (<NUM>), O-(<NUM>-azabenzotriazol-l-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (<NUM>) and N,N-diisopropylethylamine (<NUM>) were added to dichloromethane (<NUM>) and the mixture was stirred at <NUM> for <NUM> hours. When the reaction was completed, the reaction mixture was poured into saturated sodium bicarbonate aqueous solution (<NUM>) and the mixture was stirred evenly. The organic phase was separated and dried over an appropriate amount of anhydrous sodium sulfate. The desiccant was removed by filtration, and the filtrate was concentrated to dryness under reduced pressure to obtain a crude product. The crude product was purified by column chromatography (pure petroleum ether, petroleum ether: ethyl acetate = <NUM>:<NUM>, ethyl acetate: methanol= <NUM>:<NUM>) to obtain compound WX001-<NUM>.

Compound WX001-<NUM> (<NUM>) was dissolved in anhydrous tetrahydrofuran (<NUM>) and the reaction mixture was cooled to <NUM>. A solution of magnesium methyl bromide (<NUM>, <NUM>) in ether was added dropwise to the reaction system and the mixture was stirred at <NUM> for <NUM> minutes. The reaction mixture was poured into saturated ammonium chloride aqueous solution (<NUM>), and the mixture was extracted with ethyl acetate (<NUM>×<NUM>). The organic phases were combined and dried over an appropriate amount of anhydrous sodium sulfate. The desiccant was removed by filtration, and the filtrate was concentrated to dryness under reduced pressure to obtain a crude product. The crude product was purified by column chromatography (pure petroleum ether, petroleum ether: ethyl acetate = <NUM>:<NUM>, ethyl acetate: methanol= <NUM>:<NUM>), and purified by machine purification (column: Welch Xtimate C18 <NUM>*<NUM>*<NUM>; mobile phase: A: aqueous solution containing <NUM> NH<NUM>HCO<NUM>, B: acetonitrile; gradient: B%: <NUM>%-<NUM>%, <NUM> minutes) to obtain compound WX001. LCMS (ESI) m/z = <NUM> [M+H]+. <NUM>H NMR (<NUM>, DMSO-d<NUM>) δ = <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>(d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM>(m, <NUM>), <NUM>-<NUM>(m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (br s, <NUM>), <NUM> (s, <NUM>).

Each of the embodiment in the following Table <NUM> was synthesized with reference to the synthesis step of Embodiment <NUM>, except that the B4 (piperidine ring) of Step <NUM> in Embodiment <NUM> was replaced by the corresponding B fragment of the corresponding Fragment <NUM>, and the synthesis step may undergo conventional operations such as removal of Boc, hydrolysis, formation of tertiary alcohols with esters using methyl Grignard reagents or Suzuki coupling, etc..

WX016-<NUM> was synthesized with reference to the synthesis step of embodiment <NUM>, except that the piperidine in step <NUM> was replaced with <NUM>,<NUM>-dimethylpiperidine.

Compound WX016-<NUM> (<NUM>) was dissolved in a solution of sodium hydroxide (<NUM>) in water (<NUM>), then methanol (<NUM>) was added and the reaction was carried out at <NUM> for <NUM> hours. The pH value of the reaction mixture was adjusted to <NUM>-<NUM> with <NUM> hydrochloric acid, and then the mixture was extracted with ethyl acetate (<NUM>×<NUM>), and the organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. Then the residue was purified by machine purification (column: Welch Xtimate C18 <NUM>*<NUM>*<NUM>; mobile phase: [aqueous solution containing (<NUM>) NH<NUM>HCO<NUM>) - acetonitrile]; gradient: B%: <NUM>% - <NUM>%, <NUM>) to obtain compound WX016. LCMS (ESI) m/z: <NUM> [M+H]+. <NUM>H NMR (<NUM>, DMSO-d<NUM>) δ= <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>).

Each of the embodiments in the following Table <NUM> was synthesized with reference to the synthesis step of embodiment <NUM>, except that the <NUM>,<NUM>-dimethylpiperidine in step <NUM> was replaced with Fragment <NUM>.

WX021-<NUM> was synthesized with reference to the similar synthesis step of Embodiment <NUM>, except that in the step <NUM> of Embodiment <NUM>, piperidine was not used to substitute the chlorine atom.

WX020-<NUM> (<NUM>), B10 (<NUM>), toluene (<NUM>), ethanol (<NUM>) and water (<NUM>) were added to a reaction flask, then sodium bicarbonate (<NUM>) and tetrakis(triphenylphosphine)palladium (<NUM>) were added thereto. The mixture was replaced with nitrogen three times, and stirred at <NUM> for <NUM> hours. Then the mixture was filtered, and the filtrate was collected, then concentrated to dryness under reduced pressure. The crude product was purified by silica gel plate (eluent: dichloromethane: methanol = <NUM>:<NUM>) to obtain compound WX021. LCMS (ESI) m/z: <NUM>[M+H]+. <NUM>H NMR (<NUM>, DMSO-d<NUM>) <NUM>=<NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (d, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

Each of the embodiment in the following Table <NUM> was synthesized with reference to the synthesis step of Embodiment <NUM>, except that the B10 (phenyboronic acid) in the steps of Embodiment <NUM> was replaced by the corresponding B fragment of the corresponding Fragment <NUM>, and the synthesis steps may undergo simple operations such as hydrogenation and cyanohydrolysis to amide.

<NUM>-Amino-<NUM>-chloro-<NUM>-nitropyridine (<NUM>, <NUM> mmol) and ethyl bromoacetyl pyruvate (<NUM>, <NUM> mmol) were added to a reaction flask, and the mixture was replaced with nitrogen three times, and the reaction was stirred at <NUM> for <NUM> hours. Ethanol (<NUM>) was added to the reaction mixture, and the mixture was stirred for <NUM> hours, then filtered; the solid was collected, and concentrated under reduced pressure to dryness to obtain compound WX037-<NUM>.

WX037-<NUM> (<NUM>, <NUM> mmol) and anhydrous ethanol (<NUM>) were added to a reaction flask, then concentrated sulfuric acid (<NUM>, <NUM> mmol, <NUM>, <NUM>% purity) was added thereto, and the reaction mixture was stirred at <NUM> for <NUM> hours. The reaction mixture was concentrated under reduced pressure. Ethyl acetate (<NUM>) was added for dissolution, then pH value was adjusted to <NUM> with saturated sodium carbonate aqueous solution; the phases were separated, and the organic phase was dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by column chromatography (dichloromethane: methanol=<NUM>: <NUM>-<NUM>: <NUM>) to obtain WX037-<NUM>.

WX037-<NUM> (<NUM>, <NUM> mmol) and isopropyl acetate (<NUM>) were added to a reaction flask, then stannic chloride dihydrate (<NUM>, <NUM> mmol) was added thereto. The mixture was stirred at <NUM> for <NUM> hours. Ethyl acetate (<NUM>) was added to the reaction mixture, then the pH value was adjusted to <NUM> by adding ammonia water dropwise, and anhydrous sodium sulfate was added, and the sodium sulfate was stirred into a sand form; the mixture was filtered, and the filtrate was collected and concentrated to dryness under reduced pressure. The crude product was purified by column chromatography (eluent: dichloromethane: methanol=<NUM>: <NUM>-<NUM>: <NUM>) to obtain WX037-<NUM>.

A mixture of WX037-<NUM> (<NUM>, <NUM> mmol, <NUM> eq), A2 (<NUM>, <NUM> mmol), N,N-diisopropylethylamine (<NUM>, <NUM> mmol, <NUM>), <NUM>% ethyl acetate solution of tri-n-propyl cyclophosphoric anhydride (<NUM>, <NUM> mmol, <NUM>, <NUM>% purity) and THF (<NUM>) was added to a reaction flask. The mixture was stirred at <NUM> for <NUM> hours. Ethyl acetate (<NUM>) was added, then the pH value was adjusted to <NUM> with saturated sodium carbonate aqueous solution; the phases were separated, and the organic phase was collected and dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by column chromatography (dichloromethane: methanol=<NUM>: <NUM>-<NUM>: <NUM>) to obtain WX037-<NUM>.

WX037-<NUM> (<NUM>, <NUM> mmol), B19 (<NUM>, <NUM> mmol), potassium phosphate (<NUM>, <NUM> mmol) [methanesulfonic acid (<NUM>-dicyclohexylphosphine)-<NUM>,<NUM>-dimethoxy-<NUM>,<NUM>-triisopropyl-<NUM>,<NUM>-biphenyl)(<NUM>-amino-<NUM>,<NUM>-biphenyl-<NUM>-yl) palladium (II) (<NUM>, <NUM>µmol), tetrahydrofuran (<NUM>) and water (<NUM>) were added to a reaction flask, then the mixture was replaced with nitrogen three times, and stirred at <NUM> for <NUM> hours. Then the mixture was filtered, and the filtrate was collected, then concentrated to dryness under reduced pressure. The crude product was purified by column chromatography (dichloromethane: methanol=<NUM>: <NUM>-<NUM>: <NUM>) to obtain WX037-<NUM>.

WX037-<NUM> (<NUM>, <NUM>µmol), sodium hydroxide (<NUM>, <NUM>µL), methanol (<NUM>) were added to a reaction flask, and the mixture was replaced with nitrogen three times, then stirred at <NUM> for <NUM> hours. Methanol was concentrated to dryness under reduced pressure, then the pH was adjusted to <NUM> with 2N hydrochloric acid, and then the mixture was concentrated to dryness under reduced pressure. Then the crude product was purified by machine purification (column: Phenomenex Gemini NX-C18 (<NUM>*<NUM>*<NUM>); mobile phase: [aqueous solution containing (<NUM>) NH<NUM>HCO<NUM>) - acetonitrile]; gradient: B%: <NUM>% - <NUM>%, <NUM>) to obtain WX037.

<NUM>H NMR (<NUM>, DMSO-d<NUM>) δ=<NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (d, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (d, J=<NUM>, <NUM>), <NUM>-<NUM> (t, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (t, <NUM>), <NUM>-<NUM> (t, <NUM>), <NUM> (s, <NUM>).

Each of the embodiments in the following Table <NUM> was synthesized with reference to the synthesis step of embodiment <NUM>, except that the B-<NUM> in step <NUM> was replaced with Fragment <NUM>.

WX001-<NUM> (<NUM>, <NUM> mmol) was added to a flask containing ethyl bromopyruvate (<NUM>, <NUM> mmol, <NUM>) and the reaction mixture was stirred at <NUM> for <NUM> hours. The reaction mixture was poured into ethyl acetate (<NUM>) while hot, then stirred at <NUM> for <NUM> minutes, filtered under suction, and the filter cake was rinsed with ethyl acetate (<NUM> × <NUM>), and concentrated to dryness under reduced pressure to obtain WX040-<NUM>.

Raney nickel (<NUM>) was added to an argon-protected hydrogenation flask, then the flask was wetted with ethanol (<NUM>), and WX040-<NUM> (<NUM>, <NUM> mmol) was added to the reaction system; the mixture was stirred at <NUM> for <NUM> hours under <NUM> Psi hydrogen. The reaction mixture was filtered through celite under suction, and the filtrate was concentrated to dryness under reduced pressure to obtain WX040-<NUM>.

WX040-<NUM> (<NUM>, <NUM>µmol), A2 (<NUM>, <NUM>µmol), O-(<NUM>-azabenzotriazol-<NUM>-yl)-N,N,N,N-tetramethyluronium hexafluorophosphate (<NUM>, <NUM> mmol), N,N-diisopropylethylamine (<NUM>, <NUM> mmol, <NUM>µL) were added to a flask containing anhydrous dichloromethane (<NUM>), and the reaction mixture was stirred at <NUM> for <NUM> hours. The reaction mixture was poured into saturated ammonium chloride solution (<NUM>); the phases were separated, and the organic phase was dried, filtered and concentrated under reduced pressure. The crude product was eluted by column separation (petroleum ether to petroleum ether: ethyl acetate = <NUM>: <NUM> to pure ethyl acetate) to obtain WX040-<NUM>.

Methyl magnesium chloride (<NUM> mol/L, <NUM>) was added to a flask containing anhydrous tetrahydrofuran (<NUM>) under nitrogen protection, and WX040-<NUM> (<NUM>, <NUM>µmol) dissolved in anhydrous tetrahydrofuran (<NUM>) was added dropwise to the above solution at <NUM>, and the reaction was stirred at <NUM> for <NUM> hours. The reaction mixture was quenched by pouring into saturated ammonium chloride solution (<NUM>), and the mixture was extracted with ethyl acetate (<NUM> × <NUM>); the organic phases were dried, filtered and concentrated under reduced pressure. The crude product was purified by plate separation (ethyl acetate: methanol = <NUM>: <NUM>) to obtain WX040.

<NUM>H NMR (<NUM>, CD<NUM>OD-d<NUM>) δ=<NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM> <NUM>), <NUM> (s, <NUM>), <NUM> (d, J=<NUM> <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM>(m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

Each of the embodiments in the following Table <NUM> was synthesized with reference to the synthesis step of embodiment <NUM> and embodiment <NUM>, except that the piperidine at the bottom right was replaced with Fragment <NUM>.

WX042-<NUM> was synthesized with reference to the synthesis step of embodiment <NUM>, except that the A1 in step <NUM> was replaced with ethyl <NUM>-bromoacetoacetate.

Compound WX042-<NUM> (<NUM>, <NUM> mmol) was dissolved in tetrahydrofuran (<NUM>), and the mixture was cooled to -<NUM>; lithium aluminum tetrahydride (<NUM>) was added in batches to the reaction system, and the reaction was stirred at -<NUM> for <NUM> hour. The reaction mixture was poured into ammonium chloride aqueous solution (<NUM>), and the mixture was extracted with ethyl acetate (<NUM> × <NUM>), and the organic phases were combined, washed with saturated saline (<NUM>), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by column purification (dichloromethane: methanol=<NUM>: <NUM>-<NUM>: <NUM>) to obtain WX042-<NUM>.

Compound WX042-<NUM> (<NUM>) was dissolved in chloroform (<NUM>), and triethylamine (<NUM>) was added thereto, then the mixture was cooled to <NUM> and stirred for <NUM> minutes, then a solution of methanesulfonyl chloride (<NUM>) in chloroform (<NUM>) was slowly added dropwise. The reaction was slowly heated to <NUM> and stirred for <NUM> minutes. The reaction mixture was concentrated under reduced pressure to obtain WX042-<NUM>.

Compound WX042-<NUM> (<NUM>) and sodium methylsulfinate (<NUM>, <NUM>µmol) were dissolved in N,N-dimethylformamide (<NUM>), and potassium iodide (<NUM>) was added thereto. The reaction was carried out at <NUM> (0bar) for <NUM> hour in microwave instrument. The reaction mixture was diluted with <NUM> of ethyl acetate, then the mixture was poured into semi-saturated saline (<NUM>); the phases were separated, and the aqueous phase was extracted with ethyl acetate (<NUM> × <NUM>), and the organic phases were combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. Then the crude product was purified by machine purification (column: Welch Xtimate BEH C18 <NUM>*<NUM>*<NUM>; phase: A: aqueous solution containing <NUM> NH<NUM>HCO<NUM>, B: acetonitrile; gradient: B%: <NUM>%-<NUM>%, <NUM> minutes) and freeze-dried to obtain WX042.

<NUM>H NMR (<NUM>, DMSO-d<NUM>) δ= <NUM>(s, <NUM>), <NUM> (s, <NUM>), <NUM>(s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>(s, <NUM>), <NUM>(s, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM>(s, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM>(s, <NUM>).

Each of the embodiments in the following Table <NUM> was synthesized with reference to the synthesis step of embodiment <NUM>, except that the piperidine at the bottom right was replaced with Fragment <NUM>.

The IC<NUM> values were determined using <NUM>P isotope-labeled kinase activity assay (Reaction Biology Corp) to evaluate the inhibitory ability of the tested compounds on human IRAK4.

Buffer conditions: <NUM> Hepes (pH <NUM>), <NUM> MgCl<NUM>, <NUM> EGTA, <NUM>% Brij35, <NUM>/mL BSA, <NUM> Na<NUM>VO<NUM>, <NUM> DTT, <NUM>% DMSO.

Test procedure: At room temperature, the tested compound was dissolved in DMSO to prepare a <NUM> solution for later use. The substrate was dissolved in a newly prepared buffer solution, and the tested IRAK4 kinase was added thereto and mixed evenly. The DMSO solution dissolved with the tested compound was added to the above reaction mixture mixed evenly using acoustic technique (Echo <NUM>). After incubation for <NUM> minutes, <NUM>P-ATP was added to initiate the reaction. The reaction was carried out at room temperature for <NUM> minutes, and the reaction mixture was spotted on P81 ion exchange filter paper (Whatman # <NUM>-<NUM>). The filter paper was washed repeatedly with <NUM> % phosphoric acid solution, and the radioactivity of phosphorylated substrate residues on the filter paper was determined. The kinase activity data were expressed by comparing the kinase activity of the group containing the tested compound with the kinase activity of the blank group (only containing DMSO), and the IC<NUM> value was obtained by curve fitting by Prism4 software (GraphPad), and the experimental results are shown in Table <NUM>.

Conclusion: The compound of the present disclosure generally exhibits good inhibitory activity against IRAK4.

The IC<NUM> values were determined using <NUM>P isotope-labeled kinase activity assay (Reaction Biology Corp) to evaluate the inhibitory ability of the tested compounds on human BTK.

Test procedure: At room temperature, the tested compound was dissolved in DMSO to prepare a <NUM> solution for later use. The substrate was dissolved in a newly prepared buffer solution, and the tested BTK kinase was added thereto and mixed evenly. The compound dissolved in DMSO was added to the kinase reaction mixture through Echo <NUM> (Acoustic technology; Nanoliter range). After incubation for <NUM> minutes at room temperature, <NUM>P-ATP was added to initiate the reaction. The reaction was carried out at room temperature for <NUM> hours, and the radioactivity of the reaction liquid point was detected by filtration-binding method with P81 ion exchange filter paper. The kinase activity data were expressed by comparing the kinase activity of the group containing the tested compound with the kinase activity of the blank group (only containing DMSO), and the IC<NUM> value was obtained by curve fitting by Prism4 software (GraphPad), the experimental results are shown in Table <NUM>.

Conclusion: The compound of the present disclosure generally exhibits good inhibitory activity against BTK.

THP-<NUM> human acute single cell leukemia cells were purchased from ATCC (Cat # TIB-<NUM>) and cultured at <NUM> in <NUM>% CO<NUM> incubator. The composition of medium was RPMI1640 (Gibco, Cat # <NUM>-<NUM>), and the supplementary compositions were <NUM>% FBS (Gibco, Cat # <NUM>); <NUM>% PenStrep (Gibco, Cat # <NUM>); <NUM> <NUM>-Mercaptoethanol (Sigma, Cat # M6250).

TNF-α Elisa kit was used to detect the content of TNF-α in cell culture supernatant samples. TNF-α was produced by stimulating THP-<NUM> cells with <NUM> ng/mL LPS (Sigma, Cat # L6529).

THP-<NUM> cells cultured normally at logarithmic growth stage were seeded in a <NUM>-well plate (Corning # <NUM>) at a certain concentration (<NUM>*<NUM><NUM>/<NUM>µL) and then put into a cell incubator for incubation. After two hours, <NUM>µL of different concentrations of the compound to be tested (<NUM>* final concentration) were added and incubated in an incubator. After one hour, <NUM>µL of <NUM> ng/mL LPS was added and incubated in an incubator. After <NUM> hours, the culture supernatant samples were collected by centrifugation, and the content of TNF-α could be detected by TNF-α Elisa kit. Finally, OD signals (OD450-OD570) were read on envision board reader.

The OD450-OD570 signal value was converted into a percentage inhibition rate.

Inhibition rate%= (ZPE-sample)/(ZPE-HPE)*<NUM>.

"HPE" refers to the OD450-OD570 signal value of the control well without LPS stimulated cells, and "ZPE" refers to the OD450-OD570 signal value of the control well with LPS stimulated cells. The IC<NUM> value of the compound was calculated by XLFit in the excel add-in.

Equation: Y=Bottom + (Top-Bottom)/(<NUM>+(IC<NUM>/X)^HillSlope).

A summary of the test results is shown in Table <NUM>.

Conclusion: The compound of the present dsiclsoure generally exhibits better activity of inhibiting cell TNF-α generation in THP-<NUM> cell activity experiment.

OCI-LY10 human B-cell lymphoma cells were cultured in a <NUM>, <NUM>% CO<NUM> incubator. The composition of medium was IMDM (GIBCO, Cat # <NUM>); the supplementary compositions were <NUM>% FBS (Hyclone, Cat # SH30084. <NUM>); <NUM>% PenStrep (Thermo, Cat # SV30010).

TMD8 human B-cell lymphoma cells were cultured in a <NUM>, <NUM>% CO<NUM> incubator. The composition of medium was RPMI1640 (GIBCO, Cat # <NUM>-<NUM>); the supplementary compositions were <NUM> % FBS (Hyclone, Cat # SH30084. <NUM>); <NUM>% PenStrep (Thermo, Cat # SV30010).

The tumor cell lines OCI-LY10 and TMD8 were used to detect the effect of the compound on inhibiting tumor cell proliferation in vitro. The tumor cell line was cultured in a <NUM>, <NUM>% CO<NUM> incubator according to the culture conditions shown, and passaged regularly, then the cells in the logarithmic growth phase were taken, counted, and spread in a <NUM>-well plate (the cells in each well were adjusted to an appropriate concentration, a total of <NUM> cell suspensions per well was added). After incubating overnight in a <NUM>, <NUM> % CO<NUM> incubator, drugs with different concentration gradients (<NUM>µL of drug solution was added) were added and treated for <NUM> days, then <NUM>µL of CellTiter-Glo working solution was added to each well, and the cell plate was wrapped with aluminum foil to avoid light. The culture plate was shaken on an orbital shaker for <NUM> minutes to induce cell lysis, and placed at room temperature for <NUM> minutes to stabilize the luminescence signal, then the luminescence signal was detected on the <NUM> EnVision plate reader.

The Inhibition rate (IR) of the tested compound was calculated using the following formula: <MAT>.

The inhibition rates of different concentrations of the compounds were calculated in Excel, and then the inhibition curves were made by GraphPad Prism software and the related parameters were calculated, including the minimum inhibition rate, the maximum inhibition rate and IC<NUM>.

Experimental results are shown in Table <NUM>:.

Conclusion: The compounds of the present disclsoure generally exhibit good inhibitory activity on cell proliferation in OCI-LY10 and TMD-<NUM> cell lines, respectively.

The tumor cell line used in this experiment was provided by Nanjing Cobioer Biotechnology Co. See Table <NUM> below for specific information.

The tumor cell lines OCI-LY3 were used to detect the effect of the compound on inhibiting tumor cell proliferation in vitro. The OCI-LY3 cell line was cultured in the corresponding medium at <NUM> and <NUM>% CO<NUM>, and the logarithmic growth phase cells were used in the experimental plating. The cells were collected and centrifuged at <NUM> rpm for <NUM> minutes, and the culture medium was re-suspended and spread in a <NUM>-well plate. After incubating overnight in a <NUM>, <NUM> % CO<NUM> incubator, the cells with different concentration gradients (<NUM>µL of prepared diluent of the tested compound) were incubated for <NUM> hours, and the cell culture plates were incubated with CTG reagent at room temperature and away from light for <NUM> minutes, and then recovered to room temperature. <NUM>µL/hole of CTG solution was added into the biosafety cabinet away from light, and the plate shaker was shaken and mixed evenly away from light for <NUM> minutes, and incubated at room temperature away from light for <NUM> minutes. The luminescence values were read and recorded using the Perkin Elmer Envision <NUM> Multilabel Reader.

The results of luminescence values measured at each drug concentration were normalized with the luminescence values of the blank control group, and the ratio of this value to the DMSO group was taken as the cell inhibition rate (%). Using GraphPad software, the logarithm of drug concentration (log drug concentration) versus inhibition rate was plotted, and the software automatically fitted and calculated IC<NUM> value and <NUM>% confidence limit value by log (inhibitor) vs. normalized response algorithm of nonlinear regression.

Experimental results are shown in Table <NUM>.

Conclusion: The compound of the disclosure has a significant inhibition effect on cell proliferation in OCI-LY3 cell line.

SD rats were orally given the solvent, the positive drug dexamethasone (DEX, <NUM>/kg) and the tested compound, and LPS (<NUM>/kg) was intraperitoneally injected <NUM> hours after the administration. Two hours after LPS injection, the animals were euthanized by CO<NUM>, and blood samples were collected from the heart and placed in an anticoagulant tube containing EDTA-K<NUM>, then partial anticoagulated blood was centrifuged to separate the plasma and the plasma was frozen at -<NUM>.

The plasma was taken out of the refrigerator at -<NUM>, thawed at room temperature, and the concentration of TNF-α in the plasma was detected according to the ELISA kit instructions.

The experimental data were expressed by Mean ± standard error (Mean ± SEM), and the level of TNF-α was expressed by One-way ANOVA, and p <<NUM> was considered as a significant difference. The result of in vivo pharmacodynamic study of TNF-α secretion in SD rats induced by lipopolycollagen (LPS) are shown in <FIG>.

The results in <FIG> show that the SD rat orally administrated compound WX001 showed a significant inhibitory effect on TNF-α secretion induced by lipopolycollagen (LPS), and the efficacy at a dose of <NUM> mpk was equivalent to the efficacy of dexamethasone (DEX) at a dose of <NUM> mpk.

The objective of the experiment was to study the efficacy of WX001 as the test drug on human B-cell lymphoma OCI-LY <NUM> cell subcutaneous xenograft tumor in CB17 SCID mouse model.

OCI-LY10 human B-cell lymphoma cells were cultured in a <NUM>, <NUM>% CO<NUM> incubator. The composition of medium was IMDM (GIBCO, Cat # <NUM>); the supplementary compositions were <NUM> % FBS (Hyclone, Cat # SH30084. <NUM>); <NUM> % PenStrep (Thermo, Cat # SV30010).

OCI-LY10 tumor cells were cultured and passaged, and <NUM> (<NUM> × <NUM><NUM> cells) OCI-LY <NUM> cells were subcutaneously inoculated on the right back of each nude mouse (with Matrigel, volume ratio <NUM>:<NUM>), and the group administration was started when the average tumor volume reached <NUM><NUM>. The health status and death of animals were monitored every day, and routine examinations included observing the effects of tumor growth and drug treatment on daily behaviors of animals, such as behavioral activities, food intake and water intake, weight change (weight was measured twice a week), tumor size (tumor volume was measured twice a week), appearance signs or other abnormal conditions.

The experimental index was to investigate whether the tumor growth was inhibited, delayed or cured. Including the measurement of tumor volume (TV), and the calculation of the compound's anti-tumor efficacy using TGI (%) or the relative tumor proliferation rate T/C (%).

TV = <NUM>a × b<NUM>, a and b represented the long diameter and short diameter of the tumor, respectively.

T/C% = TRTV/CRTV × <NUM>% (TRTV: RTV in treatment group; CRTV: RTV in negative control group). Relative tumor volume (RTV) was calculated according to the results of tumor measurement, and the calculation formula was RTV = Vt/V<NUM>, wherein V<NUM> was the average tumor volume measured at the time of group administration (i.e., d<NUM>), Vt was the average tumor volume at a certain measurement, and TRTV and CRTV were the data taken at the same day.

The body weight of experimental animals was used as a reference index for indirect determination of drug toxicity. After <NUM> days of administration (PG-D1-D18), all mice in the experimental group showed no abnormality and showed good drug tolerance.

The effect of WX001 compound on the body weight of female CB17 SCID mouse model bearing human B-cell lymphoma OCI-LY10 cell subcutaneous xenograft tumor is shown in <FIG> and <FIG>. <FIG> shows the weight changes of mouse model bearing human B-cell lymphoma OCI-LY10 cell subcutaneous xenograft tumor after administration of WX compound. The data points represent the average body weight in the group, and the error lines represent the standard error (SEM). The relative weight change shown in <FIG> was calculated based on the animal weight at the beginning of administration. The data points represent the average body weight change percentage in the group, and the error lines represent the standard error (SEM).

<FIG> shows the tumor growth curve of mouse model bearing human B-cell lymphoma OCI-LY10 cell subcutaneous xenograft tumor after administration of WX001 compound. The data points represent the average tumor volume in the group, and the error lines represent the standard error (SEM).

In this study, we evaluated the in vivo efficacy of WX001 compound in human B-cell lymphoma OCI-LY10 cell subcutaneous xenograft tumor model. The tumor volume of each group at different time points is shown in <FIG>.

<NUM> days after the start of administration, the T/C value of the Ibrutinib (<NUM> mpk) group was <NUM>%, and the TGI value was <NUM> %, and the p value was <<NUM>. The WX001 (<NUM> mpk) group had a T/C value of <NUM> %, a TGI value of <NUM> %, and p<<NUM>; compared with the solvent control group, the WX001 (<NUM> mpk) group had a significant anti-tumor effect and was significantly better than the Ibrutinib (<NUM> mpk) group.

Claim 1:
A compound represented by formula (II), or a pharmaceutically acceptable salt thereof,
<CHM>
wherein,
R<NUM> is selected from H, F, Cl, Br, I, OH, NH<NUM>, CN, C<NUM>-<NUM> alkyl, cyclopropyl and -C(=O)-NH<NUM>, wherein the C<NUM>-<NUM> alkyl, cyclopropyl and -C(=O)-NH<NUM> are optionally substituted by <NUM>, <NUM> or <NUM> Ra;
R<NUM> is selected from thienyl, phenyl, pyridyl, cyclopropyl, cyclohexyl and
<CHM>
wherein the thienyl, phenyl, pyridyl, cyclopropyl, cyclohexyl and
<CHM>
are optionally substituted by <NUM>, <NUM>, <NUM>, <NUM> or <NUM> Rb;
T<NUM> is selected from CH<NUM>, NH and O;
R<NUM> is selected from C<NUM>-<NUM> alkyl, wherein the C<NUM>-<NUM> alkyl is optionally substituted by <NUM>, <NUM> or <NUM> Rc;
Ra is each independently selected from F, OH, NH<NUM> and CN;
Rb is each independently selected from H, D, F, Cl, Br, I, OH, NH<NUM>, CN, C<NUM>-<NUM> alkyl, COOH, -C(=O)-C<NUM>-<NUM> alkyl, -C(=O)-O-C<NUM>-<NUM> alkyl and -C(=O)-NH<NUM>, wherein the OH, NH<NUM>, C<NUM>-<NUM> alkyl, COOH, -C(=O)-C<NUM>-<NUM> alkyl, -C(=O) -O-C<NUM>-<NUM> alkyl and -C(=O)-NH<NUM> are optionally substituted by <NUM>, <NUM> or <NUM> R;
Rc is each independently selected from F, OH, NH<NUM>, CN, CH<NUM>, COOH and -SO<NUM>CH<NUM>;
R is each independently selected from F, OH, NH<NUM> and CH<NUM>.