Patent Description:
The present disclosure relates to the technical field of medical detection, and more particularly, to an enricher, an enriching system, a sample preparation system, and a sample detection system.

Medical detection is to detect samples such as the collected blood, body fluid, secretion, excrement, exfoliation and so on via visual observation, microscopy, physical, chemical, instrumental or molecular biology methods. In order to improve the detection rate of the formed components in the samples, the samples need to be enriched.

At present, enriching methods mainly include centrifugal precipitation and natural precipitation. The centrifugal precipitation method is that the sample is centrifuged to remove the supernatant and the precipitate is taken for detection; the natural precipitation method is that the sample is left standing for a certain period of time to form a precipitation, and then the supernatant is removed and a suction liquid is taken for detection. The aforementioned different enriching methods require respective corresponding sample processing devices to transfer samples or process samples, thereby increasing the cost of enriching and further increasing the detection cost of medical detection. In addition, in the aforementioned enriching methods, the enriching processing time is relatively long due to the need to transfer samples or process samples.

Therefore, how to reduce the detection cost of medical detection is an urgent problem to be solved by those skilled in the art. <CIT> describes a membrane filter structure and a cytology smear method. <CIT> describes a cell extracting method using a sample container including a pipette. <CIT> relates to a membrane filter device for plating a target cell on a slide. <CIT> relates to a cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to method of using such a cell capture system.

Accordingly, the technical problem to be solved by the present disclosure is how to reduce the detection cost of medical detection, and for this reason, the present disclosure provides an enricher, an enriching system, a sample preparation system, and a sample detection system.

To achieve the aforementioned object, the present disclosure provides the following technical solutions:
An enricher as set out in claim <NUM>.

An enriching system includes a suction mechanism and any one of the aforementioned enrichers. The suction mechanism is configured to be connected to the suction connection portion, the suction mechanism is configured to be capable of generating the negative pressure in the enriching cavity of the enricher.

A sample preparation system includes a sample transferring mechanism and any one of the aforementioned enriching system. The sample transferring mechanism is configured to transfer the retentate on the blocking member to a detection carrier.

A sample detection system includes a microscope and any one of the aforementioned sample preparation systems. The microscope is configured to microscopically examine the sample.

It can be seen from the aforementioned technical solutions that when the enricher of the present disclosure is in application, the enriching cavity is in communication with the suction mechanism via the suction connection portion, and the portion of the enriching housing provided with the blocking member is in contact with the sample. The suction mechanism operates to generate the negative pressure in the enriching cavity, and under an action of the negative pressure, the sample penetrates the blocking member to form the suction liquid entering the enriching cavity, and the retentate is retained on the blocking member. Compared with the prior art, the enricher is capable of being in contact with the sample directly to complete the enriching processing of the sample, and does not require additional sample transfer device or sample processing device, thereby reducing the cost of sample enriching and further reducing the cost of medical detection. In addition, since this enricher is adopted, there is no need to transfer samples or process samples additionally, such that the time for sample enriching processing is shortened.

In order to explain the examples of the present disclosure or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the examples or the prior art. Obviously, the accompanying drawings in the following description are only some examples of the present disclosure. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without creative efforts.

In the <FIG> is an enricher, <NUM> is a suction mechanism, <NUM> is a sample receiving device, <NUM> is a sample transferring mechanism, <NUM> is a detection carrier, <NUM> is a staining mechanism, <NUM> is an enricher storage mechanism, <NUM> is a waste recycling mechanism , <NUM> is a microscope, <NUM> is a sample adding member, <NUM> is a chromatography column, <NUM> is an enriching housing, <NUM> is a suction connection portion, <NUM> is a blocking member, <NUM> is a support portion, <NUM> is a mounting cavity, <NUM> is a cushion body member, <NUM> is an annular support surface, <NUM> is a sidewall, <NUM> is a bottom wall, <NUM> is a sidewall, <NUM> is a filtrate suction channel, <NUM> is an airflow channel, <NUM> is a power mechanism, <NUM> is a controller, <NUM> is a timer, <NUM> is a pressure sensor, 101A is a first housing, 101B is a second housing, 104A is a support column, and 104B is a limiting protrusion.

The core of present disclosure is to provide an enricher, an enriching system, a sample preparation system, a sample detection system, an enriching method, a sample preparation method and a sample detection method, so as to reduce the detection cost of medical detection.

In addition, the examples shown below do not have any limiting effect on the content of the invention described in the claims. In addition, the whole content of the composition shown in the following example is not limited to what is necessary for the solution of the invention described in the claim.

Referring to <FIG> (wherein <FIG>, <FIG>, <FIG>, <FIG> show embodiments of the present invention), an enricher disclosed in the example of the present disclosure includes:.

When the enricher <NUM> of the present disclosure is in use, the enriching cavity <NUM> is in communication with the suction mechanism <NUM> via the suction connection portion <NUM>, and a portion of the enriching housing <NUM> provided with the blocking member <NUM> is in contact with the sample. The suction mechanism <NUM> operates to form the negative pressure in the enriching cavity <NUM>, and under an action of the negative pressure, the sample penetrates the blocking member <NUM> to form the suction liquid entering the enriching cavity <NUM>, and the retentate is retained on the blocking member <NUM>. Different retentate can be obtained according to different selected blocking members <NUM>, that is, a preset retentate can be obtained. Compared with the prior art, the enricher <NUM> can complete the enriching processing of the sample by directly contacting the sample, which does not require additional sample transferring device or sample processing device, thereby reducing the cost of sample enriching and further reducing the cost of medical detection. In addition, there is no need to transfer samples or additionally process samples when using the enricher <NUM>, such that the time for sample enriching processing is shortened.

It should be noted that, a shape of the enriching housing <NUM> in the present disclosure is not specifically limited. It may be a regular structure or an irregular structure. The regular structure may be a cubic column structure, a cylindrical structure, a conical structure, and the so on. Taking the enriching housing <NUM> having a cylindrical structure as an example, the enriching housing <NUM> includes a bottom wall <NUM> and a sidewall <NUM> extending from an edge of the bottom wall <NUM> in the axial direction. The sidewall <NUM> rotates circumferentially to form a closed structure. The sidewall <NUM> has a cylindrical structure, a conical structure, or a cubic column structure. Of course, other structure types are not excluded, and it is within the protection scope of the present disclosure as long as the structure has a structure configured to bear and enclose the enriching cavity <NUM>. In <FIG>, <FIG>, <FIG> and <FIG>, the sidewall <NUM> has a cylindrical structure. In <FIG>, <FIG> and <FIG>, the sidewall <NUM> has a conical structure. When the sidewall <NUM> has a conical structure, along the axial direction of the enriching housing <NUM>, a cross-section of the enriching cavity <NUM> gradually decreases from top to bottom. This arrangement facilitates the mounting of subsequent components.

An inner surface of a housing wall of the enriching housing <NUM> corresponds to the enriching cavity <NUM>, and an outer surface of the housing wall of the enriching housing <NUM> corresponds to the outside. When the enriching housing <NUM> includes a bottom wall <NUM> and a sidewall <NUM>, the sidewall <NUM> also has an outer surface and an inner surface correspondingly, and the bottom wall <NUM> also has an outer surface and an inner surface correspondingly. Thus, the outer surface includes the outer surface in correspondence with the bottom wall <NUM> and the outer surface in correspondence with the sidewall <NUM>.

The enriching housing <NUM> can be made of plastic, resin, glass and so on, it is within the protection scope of the present disclosure as long as it has a structure enclosing the enriching cavity <NUM>.

Referring to <FIG>, in order to facilitate the extraction of the suction liquid in the enriching cavity <NUM> for corresponding detection, in the example of the present disclosure, the housing wall of the enriching housing <NUM> is provided with a suction channel <NUM> in communication with the enriching cavity <NUM>. The suction liquid in the enriching cavity <NUM> can be suctioned through the suction channel <NUM>. An opening of the suction channel <NUM> is provided on the inner surface of the enriching housing <NUM> or the outer surface of the enriching housing <NUM>. When the opening of the suction channel <NUM> is provided on the inner surface of the enriching housing <NUM>, an annular support surface <NUM> is provided at the opening to support subsequent operation components for the convenience of operation.

The suction connection portion <NUM> serves to communicate the suction mechanism <NUM> and the enriching cavity <NUM>. The suction connection portion <NUM> is a hole, an opening, a joint and other structures that are in communication with the enriching cavity <NUM>, which can be arranged on the housing wall of the enriching housing <NUM>. The specific positions of the enriching housing <NUM> may vary depending on different structures thereof, but its overall function is to communicate the suction mechanism <NUM> and the enriching cavity <NUM>, and to generate the negative pressure in the enriching cavity <NUM> under an action of the suction mechanism <NUM>. Preferably, the position of the suction connection portion <NUM> is aligned with the blocking member <NUM> directly. As such, the actual volume of the enriching cavity <NUM> is the largest, and the ability to contain the suction liquid is the strongest.

The blocking member <NUM> serves to enrich the sample. When the negative pressure is generated in the enriching cavity <NUM>, the sample is capable of penetrating the blocking member <NUM> to form the suction liquid entering the enriching cavity <NUM>, and the retentate is retained on the blocking member <NUM>. The blocking member <NUM> is provided on the enriching housing <NUM>, and is connected to the enriching housing <NUM> by adhesive, heat fusion or latching. The blocking member <NUM> is a filter membrane or a filter screen. The filter membrane or the filter screen is selected depending on the sample to be enriched. When the filter membrane is selected, a filter membrane with microporous is usually adopted, and the pore size of the filter microporous of the filter membrane is usually determined according to the formed component to be detected in the sample. The pore size correspondence of different formed component and the filter membrane with microporous can be classified according to experience, and then be selected during specific operations. The blocking member <NUM> can be one layer or more layers. Referring to <FIG>, when a multi-layer blocking member <NUM> is provided at an end of the enriching cavity, the multi-layer blocking members have apertures of different sizes, and a mounting method of the multi-layer blocking members is that the blocking member with a large aperture is sleeved on the blocking member with a small aperture.

Referring to <FIG>, the blocking member <NUM> serves to enrich the sample, that is, to retain the retentate on the blocking member <NUM>. When the enriching is completed, the retentate needs to be transferred to the sample carrier, so as to complete further detection. As such, in order to prevent the blocking member <NUM> from breaking or recessing into the enriching cavity under the action of the negative pressure, a cushion body member <NUM> is provided. The cushion body member <NUM> is provided as an elastic member capable of being in a compressed state when subjected to an external force. When the retentate is transferred, the sample carrier will be in contact with the blocking member <NUM>. During the contact process, since the cushion body member <NUM> is capable of being in a compressed state when subjected to the external force, the blocking member <NUM> can be deformed, which can effectively avoid the damage caused by the rigid contact between the detection carrier <NUM> and the blocking member <NUM>, thereby facilitating the transfer of retentate. The cushion body member <NUM> can be connected to the enriching housing <NUM> by adhesion, heat fusion, latching, snapping and so on. Alternatively, referring to <FIG>, <FIG>, <FIG>, <FIG>, <FIG>, <FIG>, <FIG>, a mounting cavity <NUM> is formed on the enriching housing <NUM>, and the cushion body member <NUM> is provided in the mounting cavity <NUM>. A part where the mounting cavity <NUM> is mounted with a blocking member <NUM>, and the cushion body member <NUM> is configured to be in contact with the blocking member <NUM>.

Referring to <FIG>, <FIG>, <FIG>, <FIG>, in order to further facilitate the transfer of the retentate on the blocking member <NUM>, at least a portion of the cushion body member <NUM> protrudes from the outer surface of the enriching housing <NUM>. When the retentate on the blocking member <NUM> is transferred, since at least a portion of the cushion body member <NUM> protrudes from the outer surface of the enriching housing <NUM>, the blocking member <NUM> in contact with the cushion body member <NUM> protrudes from the outer surface of the enriching housing under the protrusion action of the cushion body member <NUM>, such that the blocking member <NUM> will not be recessed inwardly. Besides, since the blocking member <NUM> is provided as an elastic member, when the blocking member <NUM> is in contact with the detection carrier <NUM> and transfers the retentate to the detection carrier, it is equivalent to an elastic extrusion process, most of the retentate on the blocking member <NUM> can be transferred to the detection carrier, thereby further improving the transferring effect. In this embodiment, since the positions of the suction connection portion <NUM> and the blocking member <NUM> on the enriching housing <NUM> can be adjusted relatively, the outer surface can be the overall outer surface of the housing wall of the enriching housing <NUM>. The names of the housing wall of different portions in different structures are slightly different, which are all within the scope of the present disclosure.

In this embodiment, the cushion body member <NUM> is provided as a sponge, and the sponge has the effect of absorbing moisture. By providing the sponge, the suction liquid in the enriching cavity <NUM> can be effectively prevented from flowing backward and the retentate is kept moisturizing, which further improves the enriching effect of the retentate.

In this embodiment, a support component <NUM> is further provided to support the cushion body member <NUM>, and the support component <NUM> is provided as a sheet, a honeycomb, a column and so on, as long as the structure can achieve supporting the cushion body member <NUM>, it is within the scope of protection of the present disclosure.

In this embodiment, the support component <NUM> is configured to be integrally formed with the enriching housing <NUM>, or the support component <NUM> and the enriching housing <NUM> are detachable structures. Referring to <FIG>, the support component <NUM> and the enriching housing <NUM> shown in the figures form an integral structure. Referring to <FIG>, the support component <NUM> and the enriching housing <NUM> shown in the figures are detachable structures.

Referring to <FIG>, when the support component <NUM> and the enriching housing <NUM> form an integral structure, the support component <NUM> is formed by the housing wall of the enriching housing <NUM> extending in the radial direction and/or the axial direction of the enriching housing <NUM>. Taking the enriching housing <NUM> having a cylindrical structure as an example and referring to <FIG>, <FIG>, <FIG>, and <FIG>, the support component <NUM> can be regarded as the bottom wall <NUM> (when the enriching housing does not have a bottom wall) extending from the sidewall <NUM> of the enriching housing in the radial direction, or can be regarded as the bottom wall <NUM> (when the enriching shell has a bottom wall) extending in the axial direction. When the support component <NUM> and the enriching housing <NUM> form the integral structure, the mounting cavity <NUM> is configured to be formed by enclosing the support component <NUM> and the sidewall <NUM>. For example, the support component <NUM> extends as the bottom wall <NUM> or extends as a support rib or a support grid, which may enclose with the sidewall <NUM> to form the mounting cavity <NUM>. When the support component <NUM> extends to form the bottom wall <NUM>, the bottom wall <NUM> may also be recessed to form the mounting cavity <NUM>, while the support component <NUM> is provided with a communication hole <NUM> and is in communication with the enriching cavity <NUM> via the communication hole <NUM>.

Referring to <FIG>, when the support component <NUM> and the enriching housing <NUM> are detachable structures, the support component <NUM> is connected to the housing wall of the enriching housing <NUM>. Specifically, the fixing method may be that the support component <NUM> is snapped with the housing wall of the enriching housing <NUM> or limited by other limiting members. Referring to <FIG>, <FIG>, when the support component <NUM> is snapped on the inner wall of the sidewall <NUM>, the mounting cavity <NUM> is formed by the sidewall <NUM> and the support component <NUM>.

In this example, both the bottom wall and the sidewall are the housing walls of enriching housing. Both the bottom wall and the sidewall have the inner surface and the outer surface respectively, or the inner surface of sidewall is called inner wall, the outer surface of sidewall is called the outer wall and so on, which are also referred to as the housing wall in this example.

In this example, the aforementioned description describes in detail the situation in which the support component <NUM> supports the cushion body member <NUM>, that is, both the support component <NUM> and the cushion body member <NUM> are provided in the aforementioned description. It should be noted that, as shown in <FIG>, <FIG>, <FIG> and <FIG>, only the support component <NUM> may be provided individually. At this time, the support component <NUM> is equivalent to the cushion body member <NUM>, and it can also realize the function of the cushion body member <NUM> individually, that is, to prevent the situations that the blocking member <NUM> is recessed, which is not beneficial to transfer the retentate, the blocking member <NUM> is subjected to a force and broken under an action of a negative pressure suction, and so on. Especially when the support component <NUM> is configured to be detachably connected to the enriching housing <NUM>, the support component <NUM> can be made of the same material as the cushion body member <NUM>, such as an elastic member, specifically a sponge. The sponge is mounted on the enriching housing <NUM> in a manner similar to the aforementioned cushion body member <NUM>. At this time, the support component <NUM> can also be provided as at least a portion thereof extends from the outer surface of the enriching housing <NUM>.

In conclusion, those skilled in the art should understand that although the names of the support component <NUM> and the cushion body member <NUM> in this example are different, in the case where both of them are provided, the support component <NUM> plays the role of supporting the cushion body member <NUM>, but when only the cushion body member <NUM> or the support component <NUM> is provided individually, although the names of both of them are inconsistent, it should be understood that as long as it plays a role in preventing the blocking member <NUM> from recessing or breaking, both of them are the same component, and the substantial scopes thereof are the same.

Referring to <FIG>, an enriching system of the present disclosure includes a suction mechanism <NUM> and any one of the aforementioned enrichers <NUM> in the first example. The suction mechanism <NUM> is configured to be connected to the suction connection portion <NUM>, the suction mechanism <NUM> is configured to be capable of generating the negative pressure in the enriching cavity <NUM> of the enricher <NUM>.

When the enriching system is in use, the suction connection portion <NUM> is connected to the suction mechanism <NUM>, and the enricher <NUM> is moved such that the blocking member <NUM> of the enricher <NUM> is placed below a liquid level of the sample. The suction mechanism <NUM> operates to generate the negative pressure in the enriching cavity <NUM>. The sample penetrates through the blocking member <NUM> to form the suction liquid under the action of the negative pressure and enters the enriching cavity <NUM>, and the retentate is retained on the blocking member <NUM>. Compared with the prior art, when adopting the enriching system of the present disclosure, the enricher <NUM> is capable of being in contact with the sample directly to complete the enriching processing of the sample, and does not require additional sample transfer device or sample processing device, thereby reducing the cost of sample enriching and further reducing the cost of medical detection. In addition, since this enricher <NUM> is adopted, there is no need to transfer samples or process samples additionally, such that the time for sample enriching processing is shortened.

The aforementioned suction mechanism <NUM> may be a device capable of generating the negative pressure, such as a syringe, a vacuum generator, and so on. The present disclosure preferably adopts a vacuum generator, which is convenient to realize automatic control.

In order to improve the operation accuracy, the enriching system further includes: a power mechanism <NUM> configured to drive the enricher <NUM> to move. The power mechanism <NUM> enables the enricher <NUM> to move below the liquid level of the sample, and when the enriching is completed, the power mechanism <NUM> enables the enricher <NUM> to be moved out of the liquid level of the sample.

In order to further improve operation accuracy and automation of operation, the enriching system further includes:
a controller <NUM> pre-storing a target distance of the enricher <NUM>. When the enricher <NUM> moves to the target distance, the controller <NUM> controls the power mechanism <NUM> to stop moving. At this time, a distance measuring device configured to measure the operation distance of the enricher <NUM> is further included.

It should be noted here that this target distance can be converted into a target operation time of the power mechanism <NUM> or the target number of steps of the power mechanism <NUM>. When the target distance is converted into the target operation time, the target operation time of the enricher <NUM> is pre-stored and the operation time of the power mechanism <NUM> is recorded. When the operation time of the moving enricher <NUM> is equal to the target time, the power mechanism <NUM> is controlled to stop moving. At this time, an enricher timer for recording the operation time of the enricher <NUM> is further included.

When the target distance is converted into the target number of steps, the target number of steps of the enricher <NUM> is pre-stored, and the number of operation steps of the power mechanism <NUM> is recorded. When the number of operation steps that the enricher <NUM> moves is equal to the target number of steps, the power mechanism <NUM> is controlled to stop moving. At this time, a counter configured to record the number of operation steps of the power mechanism <NUM> is further included.

Further, in order to further optimize the aforementioned solutions, a timer <NUM> is further included, and the timer <NUM> is configured to record the operation time of the suction mechanism <NUM>.

The controller <NUM> further pre-stores the target operation time of the suction mechanism <NUM>. When the operation time of the suction mechanism <NUM> reaches the target operation time, the controller <NUM> controls the suction mechanism <NUM> to stop operating.

Since the enriching system does not need to carry out the unlimited suction treatment during the operation process, by pre-storing the target operation time, the operation process of the suction mechanism <NUM> can be effectively controlled, which is convenient for automatic control and reduces the work intensity of the operator.

In order to ensure the safety during the operation, a pressure sensor <NUM> configured to collect the operation pressure of the suction mechanism <NUM> is further included.

The controller <NUM> further pre-stores a target pressure of the suction mechanism <NUM>. When the operation pressure reaches the target operation pressure, the operation of the suction mechanism <NUM> is stopped.

When the operation pressure of the suction mechanism <NUM> exceeds the target pressure, it means that the blocking member <NUM> is blocked. At this time, regardless of whether the operation time of the suction mechanism <NUM> reaches the target operation time, the operation of the suction mechanism <NUM> is stopped.

Referring to <FIG>, the sample preparation system disclosed in the example of the present disclosure includes a sample transferring mechanism <NUM> and any one of the enriching systems according to the second example. The sample transferring mechanism <NUM> is configured to transfer the retentate on the blocking member <NUM> to a detection carrier <NUM>.

When using the sample preparation system in the example of the present disclosure, the sample transferring mechanism <NUM> transfers the retentate on the enricher <NUM> after enriching by the enriching system to the detection carrier <NUM> to prepare the sample specimen. Due to adopting the sample preparation system of the present disclosure and adopting the samples after enriching processing by the enriching system, the number of times of sample transfer is reduced during the period, and thus the cost is reduced. Since there are fewer intermediate stages, the sample preparation accuracy can be improved and the detection rate of the sample can be improved.

The sample transferring mechanism <NUM> is configured to drive the enricher <NUM> to move along the vertical direction and/or the horizontal direction to be in contact with the detection carrier <NUM>.

The sample preparation system in the example of the present disclosure further includes an elution container configured to contain an elution solution to elute the retentate retained on the blocking member <NUM> of the enricher <NUM> into the elution solution to form a concentrated suspension. The sample transferring mechanism <NUM> is provided as a sample suction member to add the concentrated suspension to the detection carrier <NUM>. As such, the sample preparation can be performed according to different detections.

Because in some detection processes, the sample needs to be stained to facilitate detection, the sample preparation system in the example of the present disclosure further includes a sample staining mechanism <NUM> configured to stain the retentate on the detection carrier <NUM>. The staining mechanism <NUM> is a dry staining mechanism <NUM> or a wet staining mechanism <NUM>. The dry staining mechanism <NUM> is capable of performing dry staining on the sample, and the wet staining mechanism <NUM> is capable of performing wet staining on the sample.

The sample detection system in the example of the present disclosure further includes an enricher storage mechanism <NUM> and/or a waste recycling mechanism <NUM>. Since the enricher <NUM> is a disposable consumable, a plurality of enrichers <NUM> can be stored by providing the enricher storage mechanism <NUM>, and after application, the enricher <NUM> can be replaced with a new enricher, which is convenient for operation. The waste recycling mechanism <NUM> is configured to recycle the used enricher <NUM>, and the problem of medical pollution can be reduced by recycling the used enricher <NUM>.

Referring to <FIG>, the sample detection system disclosed in the example of the present disclosure includes a microscope <NUM> and any one of the sample preparation systems according to the third example, and the microscope <NUM> is configured to microscopically examine the specimen.

The specimen prepared by the sample preparation system in the third example is subjected to microscopic examination using the microscope <NUM>. Since the samples are subjected to enriching processing, the detection efficiency of the samples can be improved. In addition, since the number of sample transfers is reduced during the enriching process, the time for sample detection can be saved, and the shorter the time elapsed before the sample is microscopically examined, the higher and more accurate the detection rate will be during the microscopic examination.

The sample detection system in the example of the present disclosure further includes a sample adding member <NUM> and a dry chemical detection mechanism. The sample adding member <NUM> is configured to suck the suction liquid or a sample liquid in the enriching cavity <NUM>, and will add the suction liquid or the sample liquid to a chemical detection carrier. The dry chemical detection mechanism is configured to perform color recognition on the chemical detection carrier.

The aforementioned sample adding member <NUM> can absorb the suction liquid in the enriching cavity <NUM> after exiting the suction mechanism <NUM> after the enriching process; or absorb the suction liquid in the enriching cavity <NUM> during the enriching process. Referring to <FIG>, during the enriching process, the sample adding member <NUM> is connected to the suction connection portion <NUM> of the enricher <NUM>. The sample adding member <NUM> is connected to the suction mechanism <NUM>, and the enricher <NUM> is moved such that the blocking member <NUM> of the enricher <NUM> is completely immersed under the liquid level of the sample in the sample receiving device <NUM>. The suction mechanism <NUM> operates, such that a negative pressure state is formed in the inner cavity of the sample adding member <NUM>, and the sample in the sample receiving device <NUM> enters the enriching cavity <NUM> through the blocking member <NUM>, and then enters the inner cavity of the sample adding member <NUM>. When dry chemical detection of the suction liquid is required, the suction mechanism <NUM> drives the sample adding member <NUM> to exit the enricher <NUM>, and then the sample adding member <NUM> adds the suction liquid in the cavity to a chemical detection carrier <NUM> for color recognition.

Referring to <FIG>, when the enricher <NUM> is provided with a suction channel <NUM>, the sample adding member <NUM> is connected to the suction connection portion <NUM> of the enricher <NUM>, and the outer surface of the sample adding member <NUM> blocks the opening of the suction channel <NUM> on the inner surface of the enriching housing <NUM>. The sample adding member <NUM> is connected to the suction mechanism <NUM>, and the enricher <NUM> is moved such that the blocking member <NUM> of the enricher <NUM> is completely immersed below the liquid level of the sample in the sample receiving device <NUM>. The suction mechanism <NUM> operates, such that the negative pressure state is formed in the inner cavity of the sample adding member <NUM>. The sample in the sample receiving device <NUM> enters the enriching cavity <NUM> through the blocking member <NUM>. When the suction liquid needs to be sucked after the enriching is completed, the enricher <NUM> is rotated, such that the opening of the suction channel <NUM> located on the inner surface of the enriching housing <NUM> is opened. At this time, the suction mechanism <NUM> operates because the enriching cavity <NUM> is in communication with the outside. The suction liquid in the enriching cavity <NUM> is capable of entering the inner cavity of the sample adding member <NUM> smoothly. When dry chemical detection of the suction liquid is required, the suction mechanism <NUM> drives the sample adding member <NUM> to exit the enricher <NUM>, and then the sample adding member <NUM> adds the suction liquid in the cavity to the chemical detection carrier <NUM> for color recognition.

Referring to <FIG>, the sample detection system in the example of the present disclosure further includes a chromatography column <NUM> configured to separate target molecules in the pumped liquid by column chromatography. In use, the chromatography column <NUM> is placed in the enriching cavity <NUM>.

Referring to <FIG>, the enriching method disclosed in the example of the present disclosure, the applied enriching system includes a suction mechanism <NUM> and an enricher <NUM>. The suction mechanism <NUM> is configured to be connected to the suction connection portion <NUM> and generate the negative pressure in the enriching cavity <NUM> of the enricher <NUM>. The enricher in the enriching system includes an enriching housing <NUM>, a suction connection portion <NUM> and a blocking member <NUM>. The enriching housing <NUM> encloses to form an enriching cavity <NUM> configured to receive the suction liquid. The suction connection portion <NUM> is configured to be in communication with the suction mechanism <NUM> and the enriching cavity <NUM>, such that the negative pressure is formed in the enriching cavity <NUM> under an action of a vacuuming mechanism. The blocking member <NUM> is provided on the enriching housing <NUM>. When the negative pressure is formed in the enriching cavity <NUM>, the sample can form the suction liquid through the blocking member <NUM> and enter the enriching cavity <NUM>, and the retentate is retained on the blocking member <NUM>.

Specifically, the enriching method includes the following steps:
In S1, the suction connection portion <NUM> of the enricher <NUM> is connected to the suction mechanism <NUM>.

In the connecting process, the suction connection portion <NUM> is connected to the suction mechanism <NUM> via moving the enricher <NUM>, or the suction connection portion <NUM> is connected to the suction mechanism <NUM> via moving the suction mechanism <NUM>, or the suction connection portion <NUM> is connected to the suction mechanism <NUM> by moving the enricher <NUM> and the suction mechanism <NUM> simultaneously. The aforementioned moving process can be performed manually or by the power mechanism <NUM>.

In S2, the enricher <NUM> is moved to be immersed in the sample liquid in the sample receiving device <NUM>.

The enricher <NUM> is moved by manual operation, and moved by automatic control. When automatic control is required to move, the step S2 includes that the target distance of the enricher <NUM> is pre-stored, and when the enricher <NUM> moves the target distance, the enricher <NUM> is stopped moving. As a structural support, the enriching system includes a controller <NUM> and a power mechanism <NUM>. The power mechanism <NUM> is configured to drive the enricher <NUM> to move, and the enricher <NUM> is capable of being moved below the liquid level of the sample via the power mechanism <NUM>. When the enriching is completed, the enricher <NUM> is capable of being moved out of the liquid level of the sample via the power mechanism <NUM>. The controller <NUM> pre-stores the target distance of the enricher <NUM>. When the enricher moves the target distance, the power mechanism <NUM> is controlled to stop moving. At this time, a distance measuring device configured to measure the operation distance of the enricher <NUM> is further provided.

It should be noted here that this target distance can be converted into the target operation time of the power mechanism <NUM> or the target number of steps of the power mechanism <NUM>. When the target distance is converted into the target operation time, step S2 includes that the target operation time of the enricher <NUM> is pre-stored and the operation time of the power mechanism <NUM> is recorded, and when the operation time that the enricher <NUM> moves is equal to the target time, the power mechanism <NUM> is controlled to stop moving. In this time, the enriching system includes a timer <NUM> configured to record the operation time of the enricher <NUM>.

When the target distance is converted into the target number of steps, step S2 includes that the target number of steps of the enricher <NUM> is pre-stored and the number of operation steps of the power mechanism <NUM> is recorded, and when the number of operation steps that the enricher <NUM> moves is equal to the target number of steps, the power mechanism <NUM> is controlled to stop moving. At this time, the enriching system includes a counter configured to record the number of operation steps of the power mechanism <NUM>.

In S3, the suction mechanism <NUM> is operated, such that the sample liquid enters the enriching cavity <NUM> through the blocking member <NUM>.

The operation of the suction mechanism <NUM> is performed by manual operation, performed by automatic control. When the operation is performed by automatic control, the step S3 includes that the target operation time of the suction mechanism <NUM> is pre-stored, and when the operation time of the suction mechanism <NUM> reaches the target operation time, the operation of the suction mechanism <NUM> is stopped. The enriching system includes a timer <NUM> configured to record the operation time of the suction mechanism <NUM>. The controller <NUM> also pre-stores the target operation time of the suction mechanism <NUM>. When the operation time of the suction mechanism <NUM> reaches the target operation time, the suction mechanism <NUM> is controlled to stop operating. Since the enriching system does not need to perform unlimited suction during the operation, the operation process of the suction mechanism <NUM> can be effectively controlled by pre-storing the target operation time, which facilitates automatic control and reduces the operator's work intensity.

In order to further ensure the safety of the device, the step S3 further includes that an early warning pressure of the suction mechanism <NUM> is pre-stored, the operation pressure of the suction mechanism <NUM> is collected, and when the operation pressure reaches the early warning pressure, the operation of the suction mechanism <NUM> is stopped. The enriching system includes a pressure sensor <NUM> configured to collect the operation pressure of the suction mechanism <NUM>. The controller <NUM> also pre-stores the target pressure of the suction mechanism <NUM>. When the operation pressure reaches the target operation pressure, the operation of the suction mechanism <NUM> is stopped. When the operation pressure of the suction mechanism <NUM> exceeds the target pressure, it means that the blocking member <NUM> is blocked. At this time, regardless of whether the operation time of the suction mechanism <NUM> reaches the target operation time, the suction mechanism <NUM> stops and continues to operate.

In this example, after step S2 and before step S3, the enriching method further includes that air is blown into the sample liquid in the sample container. Clean gas is first blown into the sample liquid via the air generator, such that the sample liquid forms convection under an action of an airflow, such that the sample liquid becomes a suspension, and the formed components originally deposited at the bottom under an action of the gravity are distributed in each layer of the sample liquid, such that more formed components can be collected during subsequent enriching.

There are various ways of blowing air. A blowing unit may be provided individually to be inserted into the sample liquid to blow air, or it may be the aforementioned enricher, which discharges clean air from the enriching cavity to the sample liquid via a suction mechanism, it is also possible to use the enricher as shown in <FIG>, and air is blown through the air flow channel on the enricher.

Referring to <FIG>, the sample preparation method disclosed in the example of the present disclosure includes any one of the enriching methods according to the fifth example, and after step S3, the enriching method further includes:
In S4, the retentate retained by the blocking member <NUM> is transfer to the detection carrier <NUM>.

In this step, the retentate retained on the blocking member <NUM> is transferred to the detection carrier <NUM> by manual operation, or the retentate retained on the blocking member <NUM> is transferred to the detection carrier <NUM> by automatic control. The transferring of the retentate is realized by moving the enricher <NUM>, or by moving the detection carrier <NUM>.

In an example of the present disclosure, the step S4 includes that the enricher <NUM> is moved in a vertical direction, and the blocking member <NUM> of the enricher <NUM> is in contact with the detection carrier <NUM>; and/or the enricher <NUM> is moved in a horizontal direction, and the blocking member <NUM> of the enricher <NUM> is in contact with the detection carrier <NUM>. Specifically, the aforementioned steps are realized by providing the sample transferring mechanism <NUM>.

Further, in order to carry out different detections, the step S4 further includes that the retentate retained on the blocking member <NUM> of the enricher <NUM> is eluted in an elution solution to form a concentrated suspension. The concentrated suspension is added to the detection carrier <NUM>. The corresponding sample preparation system is realized by providing the elution vessel. The elution container is configured to contain an elution solution to elute the retentate retained on the blocking member <NUM> of the enricher <NUM> into the elution solution to form a concentrated suspension. The sample transferring mechanism <NUM> is provided as a sample suction member to add the concentrated suspension onto the detection carrier <NUM>. In this way, sample preparation can be performed according to different detections.

Since in some detection processes, the sample needs to be stained to facilitate detection, after step S4, the sample preparation method further includes that the retentate on the detection carrier <NUM> is stained. The corresponding sample preparation system is realized by providing a sample staining mechanism <NUM> configured to stain the retentate on the pair of detection carriers <NUM>. The staining mechanism <NUM> is a dry staining mechanism <NUM> or a wet staining mechanism <NUM>. The dry staining mechanism <NUM> is capable of performing dry staining on the sample, and the wet staining mechanism <NUM> is capable of performing wet staining on the sample.

In order to facilitate subsequent operations after the sample preparation is completed, the step S4 further includes that the enricher <NUM> is discarded and the enricher <NUM> is replaced with a new one. The discarded enricher <NUM> is stored in the waste recycling mechanism <NUM>, and the problem of medical pollution can be reduced by recycling the used enricher <NUM>. Further, a plurality of enrichers <NUM> may be pre-stored to facilitate replacement of new enricher <NUM>.

Referring to <FIG>, a sample detection method disclosed in the example of the present disclosure includes any one of the specimen preparation methods according to the sixth example, and after step S4 of specimen preparation method, the method further includes:
In step S5, microscopic examination is performed on the detection carrier <NUM>. The microscopic examination is performed by the microscope <NUM>.

Further, in addition to being capable of performing microscopic examination, the sample after enriching in the fifth example can also be performed by other detections. For example, this sample detection method further includes dry chemical detection. The dry chemical detection includes that the suction liquid or the sample liquid in the enriching cavity <NUM> is sucked, and is added to the chemical detection carrier <NUM> to perform chemical color detection. After the enriching processing is completed, the suction mechanism <NUM> exits and the suction liquid in the enriching cavity <NUM> is sucked. Or during the enriching processing, the suction liquid in the enriching cavity <NUM> is sucked. Referring to <FIG>, during the enriching process, the sample adding member <NUM> is connected to the suction connection portion <NUM> of the enricher <NUM>. The sample adding member <NUM> is connected to the suction mechanism <NUM>, and the enricher <NUM> is moved such that the blocking member <NUM> of the enricher <NUM> is completely immersed under the liquid level of the sample in the sample receiving device <NUM>. The suction mechanism <NUM> operates, such that a negative pressure state is formed in the inner cavity of the sample adding member <NUM>, and the sample in the sample receiving device <NUM> enters the enriching cavity <NUM> through the blocking member <NUM>, and then enters the inner cavity of the sample adding member <NUM>. When dry chemical detection of the suction liquid is required, the suction mechanism <NUM> drives the sample adding member <NUM> to exit the enricher <NUM>, and then the sample adding member <NUM> adds the suction liquid in the cavity to the chemical detection carrier <NUM> for color recognition.

Referring to <FIG>, when the enricher <NUM> is provided with a suction channel <NUM>, the sample adding member <NUM> is connected to the suction connection portion <NUM> of the enricher <NUM>, and the outer surface of the sample adding member <NUM> blocks the opening of the suction channel <NUM> located on the inner surface of the enriching housing <NUM>. The sample adding member <NUM> is connected to the suction mechanism <NUM>, and the enricher <NUM> is moved such that the blocking member <NUM> of the enricher <NUM> is completely immersed below the liquid level of the sample in the sample receiving device <NUM>. The suction mechanism <NUM> operates, such that the negative pressure state forms in the inner cavity of the sample adding member <NUM>, and the sample in the sample receiving device <NUM> enters the enriching cavity <NUM> through the blocking member <NUM>. When the suction liquid needs to be sucked after the enriching is completed, the enricher <NUM> is rotated, such that the opening of the suction channel <NUM> located on the inner surface of the enriching housing <NUM> is opened. At this time, the suction mechanism <NUM> operates because the enriching cavity <NUM> is in communication with the outside. The suction liquid in the enriching cavity <NUM> is capable of entering the inner cavity of the sample adding member <NUM> smoothly. When dry chemical detection of the suction liquid is required, the suction mechanism <NUM> drives the sample adding member <NUM> to exit the enricher <NUM>, and then the sample adding member <NUM> adds the suction liquid in the cavity to the chemical detection carrier <NUM> for color recognition.

Further, in addition to being capable of performing microscopic examination, the sample after enriching in the fifth example can also be performed by other detections. The sample detection method further includes chromatographic column <NUM> processing. The chromatographic column <NUM> processing includes that the chromatographic column <NUM> is placed in the suction liquid of the enriching cavity <NUM>, and the target molecules in the suction liquid are separated by column chromatography.

As shown in <FIG>, the difference between the enricher in this example and the enricher in the first example is that the enriching housing <NUM> includes a first housing 101A and a second housing 101B. The suction connection portion <NUM> is provided on the first housing 101A, the second housing 101B is configured to be detachably connected to the first housing 101A. After the enriching housing <NUM> is configured in two parts, the support component <NUM> is provided as a support column 104A or a limiting protrusion 104B, or the support component <NUM> includes both the support column <NUM> and the limiting protrusion 104B for better support of the cushion body member <NUM>. The support column 104A is formed by the inner wall of the first housing 101A extends in the axial direction. The cross section of the support column 104A has a grid shape to facilitate mold preparation, which is specifically similar to the shape of a Chinese character "<IMG>" or a Chinese character "<IMG>". The limiting protrusion 104B is formed by the inner wall of the second housing 101B extending in the radial direction and is configured to support the middle portion of the cushion body member <NUM>. It forms a ring shape protruding from the inner wall of the second housing 101B and is configured to support the peripheral portion of the cushion body member <NUM>. In this example, the first housing 101A and the second housing 101B are arranged by sleeving with each other. Specifically, the inner wall of the second housing 101B is sleeved around the outer wall of the first housing 101A in an interference fit to prevent disengaging from each other, or the two are glued together to prevent disengaging from each other. In this example, the enriching housing <NUM> is split into a plurality of parts, which is beneficial to the subsequent processes such as the production, processing, and assembly of the enricher. First, after the enriching housing is split, the production mold of each constituent component has a simple structure, such that the mold processing cost is low, the service life is long, and each constituent component has a simple structure in the production process to be demoulded easily, which facilitates mass production and high yield rate, and facilitates assemble and processing.

As shown in <FIG>, the difference between the enricher in this example and the enricher in the first example is that the sidewall of the enriching housing <NUM> is provided with an airflow channel <NUM>, and the airflow channel <NUM> is isolated from and not in communication with the enriching cavity <NUM>. The inlet and the outlet of the airflow channel <NUM> are both provided on the enriching housing. The airflow channel <NUM> is in communication with the air generator. The air generator generates airflow to enter the airflow channel <NUM>. When the enricher in this example extends into the sample liquid for enriching, the air generator is configured to blow clean gas into the sample liquid before enriching, such that the sample liquid forms convection under the action of the airflow, such that the sample liquid forms the suspension. The formed components originally deposited at the bottom under the action of the gravity are distributed in each layer of the sample liquid, such that more formed components can be collected during subsequent enriching. In this example, the inlet of the airflow channel <NUM> may be arranged at the top end or the sidewall of the enriching housing <NUM>, and the outlet can be arranged at the sidewall or the bottom end of the enriching housing <NUM>. The positions of the inlet and the outlet can be arbitrarily combined. The airflow channel <NUM> communicate the inlet and the outlet. In this example, although a negative pressure generator is capable of blowing air through the enriching cavity, providing the air flow channel <NUM> individually to blow air is more fluent and effective, and will not damage the connection of the blocking member.

In the description of the present disclosure, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present disclosure and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or be construed and operated in a specific orientation, therefore should not be construed as a limitation of the present disclosure. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

Claim 1:
An enricher (<NUM>), comprising:
an enriching housing (<NUM>) enclosing an enriching cavity (<NUM>) configured to receive a suction liquid, wherein the enriching housing (<NUM>) is provided with a suction connection portion (<NUM>) configured to be in communication with a suction mechanism (<NUM>) and the enriching cavity (<NUM>), such that a negative pressure is generated in the enriching cavity (<NUM>) under an action of the suction mechanism (<NUM>); wherein the enriching housing (<NUM>) includes a bottom wall (<NUM>) and a sidewall (<NUM>) extending from an edge of the bottom wall (<NUM>) in an axial direction, the enriching housing (<NUM>) is further provided with a cushion body member (<NUM>) ;and
a blocking member (<NUM>) provided on the cushion body member (<NUM>), wherein when negative pressure is generated in the enriching cavity (<NUM>), a sample is capable of penetrating the blocking member (<NUM>) to form the suction liquid entering the enriching cavity (<NUM>), and a retentate is retained on the blocking member (<NUM>); wherein the cushion body member (<NUM>) is configured to prevent the blocking member(<NUM>) from recessing or breaking;
characterized in that a middle portion of the cushion body member (<NUM>) protrudes beyond an outer surface of the bottom wall (<NUM>).