Patent Description:
Many current medicines suffer from poor absorption, distribution, metabolism and/or excretion (ADME) properties that prevent their wider use or limit their use in certain indications. Poor ADME properties are also a major reason for the failure of drug candidates in clinical trials. While formulation technologies and prodrug strategies can be employed in some cases to improve certain ADME properties, these approaches often fail to address the underlying ADME problems that exist for many drugs and drug candidates. One such problem is rapid metabolism that causes a number of drugs, which otherwise would be highly effective in treating a disease, to be cleared too rapidly from the body. A possible solution to rapid drug clearance is frequent or high dosing to attain a sufficiently high plasma level of drug. This, however, introduces a number of potential treatment problems such as poor patient compliance with the dosing regimen, side effects that become more acute with higher doses, and increased cost of treatment. A rapidly metabolized drug may also expose patients to undesirable toxic or reactive metabolites.

Another ADME limitation that affects many medicines is the formation of toxic or biologically reactive metabolites. As a result, some patients receiving the drug may experience toxicities, or the safe dosing of such drugs may be limited such that patients receive a suboptimal amount of the active agent. In certain cases, modifying dosing intervals or formulation approaches can help to reduce clinical adverse effects, but often the formation of such undesirable metabolites is intrinsic to the metabolism of the compound.

In some select cases, a metabolic inhibitor will be co-administered with a drug that is cleared too rapidly. Such is the case with the protease inhibitor class of drugs that are used to treat HIV infection. The FDA recommends that these drugs be co-dosed with ritonavir, an inhibitor of cytochrome P450 enzyme 3A4 (CYP3A4), the enzyme typically responsible for their metabolism (see <NPL>). Ritonavir, however, causes adverse effects and adds to the pill burden for HIV patients who must already take a combination of different drugs. Similarly, the CYP2D6 inhibitor quinidine has been added to dextromethorphan for the purpose of reducing rapid CYP2D6 metabolism of dextromethorphan in a treatment of pseudobulbar affect. Quinidine, however, has unwanted side effects that greatly limit its use in potential combination therapy (see <NPL>; and FDA label for quinidine at www. accessdata.

In general, combining drugs with cytochrome P450 inhibitors is not a satisfactory strategy for decreasing drug clearance. The inhibition of a CYP enzyme's activity can affect the metabolism and clearance of other drugs metabolized by that same enzyme. CYP inhibition can cause other drugs to accumulate in the body to toxic levels.

A potentially attractive strategy for improving a drug's metabolic properties is deuterium modification. In this approach, one attempts to slow the CYP-mediated metabolism of a drug or to reduce the formation of undesirable metabolites by replacing one or more hydrogen atoms with deuterium atoms. Deuterium is a safe, stable, non-radioactive isotope of hydrogen. Compared to hydrogen, deuterium forms stronger bonds with carbon. In select cases, the increased bond strength imparted by deuterium can positively impact the ADME properties of a drug, creating the potential for improved drug efficacy, safety, and/or tolerability. At the same time, because the size and shape of deuterium are essentially identical to those of hydrogen, replacement of hydrogen by deuterium would not be expected to affect the biochemical potency and selectivity of the drug as compared to the original chemical entity that contains only hydrogen.

Over the past <NUM> years, the effects of deuterium substitution on the rate of metabolism have been reported for a very small percentage of approved drugs (see, e.g., <NPL>; <NPL> ("Foster"); <NPL>; <NPL> ("Fisher")). Many of the examples in these references report a local deuterium isotope effect (an effect on the rate of metabolism at a specific site of deuteration in the substrate) rather than the effect of deuteration on the overall metabolic stability of the drug, i.e., the overall substrate consumption via metabolism. The reported results of those studies measuring deuterium substitution's effect on overall metabolic stability are variable and unpredictable. For some compounds deuteration caused decreased metabolic clearance in vivo. For others, there was no change in metabolism. Still others demonstrated increased metabolic clearance. The variability in deuterium effects has also led experts to question or dismiss deuterium modification as a viable drug design strategy for inhibiting adverse metabolism (see Foster at p. <NUM> and Fisher at p.

The effects of deuterium modification on a drug's metabolic properties are not predictable even when deuterium atoms are incorporated at known sites of metabolism. Only by actually preparing and testing a deuterated drug can one determine if and how the rate of metabolism will differ from that of its non-deuterated counterpart. See, for example, <NPL>). Many drugs have multiple sites where metabolism is possible. The site(s) where deuterium substitution is required and the extent of deuteration necessary to see an effect on metabolism, if any, will be different for each drug.

Ruxolitinib phosphate, is a heteroaryl-substituted pyrrolo[<NUM>,<NUM>-d]pyrimidines also known as <NUM>(R)-cyclopentyl-<NUM>-[<NUM>-(<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)-<NUM>-pyrazol-<NUM>-yl]propanenitrile phosphate and as (R)-<NUM>-(<NUM>-(<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-cyclopentylpropanenitrile phosphate, inhibits Janus Associated Kinases (JAKs) JAK1 and JAK2. These kinases mediate the signaling of a number of cytokines and growth factors important for hematopoiesis and immune function. JAK signaling involves recruitment of STATs (signal transducers and activators of transcription) to cytokine receptors, activation and subsequent localization of STATs to the nucleus leading to modulation of gene expression.

Ruxolitinib phosphate is currently approved for the treatment of patients with intermediate or high-risk myelofibrosis, including primary myelofibrosis, post-polycythemia vera myelofibrosis and post-essential thrombocythemia myelofibrosis. Ruxolitinib phosphate is also currently in clinical trials for the treatment of essential thrombocythemia, pancreatic cancer, prostate cancer, breast cancer, leukemia, non-Hodgkin's lymphoma, multiple myeloma and psoriasis.

Three metabolites in humans have been identified as active, that resulting from hydroxylation at the <NUM>-position on the cyclopentyl moiety, that resulting from hydroxylation at the <NUM>-position on the cyclopentyl moiety and the ketone resulting from further oxidation at the <NUM>-position on the cyclopentyl moiety. (See <NPL>; FDA Prescribing Information and <CIT>).

The most common hematologic adverse reactions associated with the dosing of ruxolitinib are thrombocytopenia and anemia. The most common non-hematologic adverse reactions are bruising, dizziness and headache.

Despite the beneficial activities of ruxolitinib, there is a continuing need for new compounds to treat the aforementioned diseases and conditions.

As defined in the attached claims, this invention relates to pharmaceutically acceptable salts of a novel deuterated derivative of ruxolitinib. This invention also provides compositions comprising a pharmaceutically acceptable salt of this invention and the use of such compositions in methods of treating diseases and conditions that are beneficially treated by administering an inhibitor of Janus-associated kinase with selectivity for subtypes <NUM> and <NUM> (JAK1/JAK2).

The term "treat" means decrease, suppress, attenuate, diminish, arrest, or stabilize the development or progression of a disease (e.g., a disease or disorder delineated herein), lessen the severity of the disease or improve the symptoms associated with the disease.

"Disease" means any condition or disorder that damages or interferes with the normal function of a cell, tissue, or organ.

It will be recognized that some variation of natural isotopic abundance occurs in a synthesized compound depending upon the origin of chemical materials used in the synthesis. Thus, a preparation of ruxolitinib will inherently contain small amounts of deuterated isotopologues. The concentration of naturally abundant stable hydrogen and carbon isotopes, notwithstanding this variation, is small and immaterial as compared to the degree of stable isotopic substitution of the compound of this invention (the deuterated derivative of ruxolitinib defined in the attached claims). See, for instance, <NPL>; <NPL>.

In the compound of this invention any atom not specifically designated as a particular isotope is meant to represent any stable isotope of that atom. Unless otherwise stated, when a position is designated specifically as "H" or "hydrogen", the position is understood to have hydrogen at its natural abundance isotopic composition. Also, when a position is designated specifically as "D" or "deuterium", the position is understood to have deuterium at an abundance that is at least <NUM> times greater than the natural abundance of deuterium, which is <NUM>% (i.e., at least <NUM>% incorporation of deuterium).

The term "isotopic enrichment factor" as used herein means the ratio between the isotopic abundance and the natural abundance of a specified isotope.

In other embodiments, the compound of this invention has an isotopic enrichment factor for each designated deuterium atom of at least <NUM> (<NUM>% deuterium incorporation), at least <NUM> (<NUM>% deuterium incorporation), at least <NUM> (<NUM>% deuterium incorporation), or at least <NUM> (<NUM>% deuterium incorporation).

The term "isotopologue" refers to a species in which the chemical structure differs from the compound of this invention only in the isotopic composition thereof.

The term "compound," when referring to the compound of this invention, refers to a collection of molecules having an identical chemical structure, except that there may be isotopic variation among the constituent atoms of the molecules. Thus, it will be clear to those of skill in the art that a compound represented by a particular chemical structure containing indicated deuterium atoms, will also contain lesser amounts of isotopologues having hydrogen atoms at one or more of the designated deuterium positions in that structure. The relative amount of such isotopologues in the compound of this invention will depend upon a number of factors including the isotopic purity of deuterated reagents used to make the compound and the efficiency of incorporation of deuterium in the various synthesis steps used to prepare the compound. However, as set forth above the relative amount of such isotopologues in toto will be less than <NUM>% of the compound. In other embodiments, the relative amount of such isotopologues in toto will be less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, or less than <NUM>% of the compound.

As defined in the attached claims, the invention relates to pharmaceutically acceptable salts of the compound of the invention. A salt of the compound of this invention is formed between an acid and a basic group of the compound or a base and an acidic group of the compound. A pharmaceutically acceptable salt may be a pharmaceutically acceptable acid addition salt.

The term "pharmaceutically acceptable," as used herein, refers to a component that is, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and other mammals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. A "pharmaceutically acceptable salt" means a non-toxic salt that, upon administration to a recipient, is capable of providing, either directly or indirectly, the compound of this invention. A "pharmaceutically acceptable counterion" is an ionic portion of a salt that is not toxic when released from the salt upon administration to a recipient.

Acids commonly employed to form pharmaceutically acceptable salts include inorganic acids such as hydrogen bisulfide, hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid and phosphoric acid, as well as organic acids such as para-toluenesulfonic acid, salicylic acid, tartaric acid, bitartaric acid, ascorbic acid, maleic acid, besylic acid, fumaric acid, gluconic acid, glucuronic acid, formic acid, glutamic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, lactic acid, oxalic acid, para-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid and acetic acid, as well as related inorganic and organic acids. Thus, as defined in the attached claims, thepharmaceutically acceptable salts of the present invention are sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caprate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, butyne-<NUM>,<NUM>-dioate, hexyne-l,<NUM>-dioate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, terephthalate, sulfonate, xylene sulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, β-hydroxybutyrate, glycolate, maleate, tartrate, methanesulfonate, propanesulfonate, naphthalene-<NUM>-sulfonate, naphthalene-<NUM>-sulfonate and mandelate salts. In one embodiment, pharmaceutically acceptable acid addition salts include those formed with mineral acids such as hydrochloric acid and hydrobromic acid, and especially those formed with organic acids such as maleic acid.

The compound of the present invention contains an asymmetric carbon atom. Accordingly, the compound of the present invention may exist as an individual stereoisomer that is substantially free from the other possible stereoisomer. The term "substantially free from the other stereoisomer" as used herein means less than <NUM>% of the other stereoisomer, preferably less than <NUM>% of other stereoisomer, more preferably less than <NUM>% of other stereoisomer and most preferably less than <NUM>% of other stereoisomer is present. Methods of obtaining or synthesizing an individual enantiomer for a given compound are known in the art and may be applied as practicable to final compounds or to starting material or intermediates.

The term "mammal" as used herein includes a human or a non-human animal, such as mouse, rat, guinea pig, dog, cat, horse, cow, pig, monkey, chimpanzee, baboon, or rhesus. In one embodiment, the mammal is a non-human animal. In another embodiment, the mammal is a human.

The term "stable compounds," as used herein, refers to compounds which possess stability sufficient to allow for their manufacture and which maintain the integrity of the compound for a sufficient period of time to be useful for the purposes detailed herein (e.g., formulation into therapeutic products, intermediates for use in production of therapeutic compounds, isolatable or storable intermediate compounds, treating a disease or condition responsive to therapeutic agents).

"D" and "d" both refer to deuterium. "Stereoisomer" refers to both enantiomers and diastereomers. "Tert" and "t-" each refer to tertiary. "US" refers to the United States of America.

"Substituted with deuterium" refers to the replacement of one or more hydrogen atoms with a corresponding number of deuterium atoms.

Throughout this specification, a variable may be referred to generally (e.g.,"each R") or may be referred to specifically (e.g., R<NUM>, R<NUM>, R<NUM>, etc.). Unless otherwise indicated, when a variable is referred to generally, it is meant to include all specific embodiments of that particular variable.

The present invention in one embodiment provides a pharmaceutically acceptable salt of the compound of Formula I as defined in the attached claims.

Thus, the present invention in one embodiment provides a pharmaceutically acceptable salt of a compound of Formula I:
<CHM>
wherein:
Y<NUM>,Y<NUM> and Y<NUM> are each hydrogen and the compound is the compound (Cmpd) set forth in the table below:.

The synthesis of the compound of Formula I may be readily achieved by synthetic chemists of ordinary skill by reference to the Exemplary Synthesis and Examples disclosed herein. Relevant procedures analogous to those of use for the preparation of the compound of Formula I and intermediates thereof are disclosed, for instance, in <CIT> and in <NPL>.

Such methods can be carried out utilizing corresponding deuterated and optionally, other isotope-containing reagents and/or intermediates to synthesize the compounds delineated herein, or invoking standard synthetic protocols known in the art for introducing isotopic atoms to a chemical structure.

The compounds of Formula I may be prepared in a manner analogous to those syntheses presented in <CIT> and in <NPL> using appropriately deuterated starting materials.

Other deuterated derivatives of ruxolitinib may be prepared as shown in the schemes below. <CHM>
<CHM>.

Scheme <NUM> discloses an exemplary preparation of compound <NUM> (wherein Y<NUM>, each Y<NUM> and each Y<NUM> are deuterium and Y<NUM>, each Y<NUM>, Y<NUM>, Y<NUM> and Y<NUM> are hydrogen). In a manner analogous to that described in <CIT>, commercially available, <NUM>-chloro-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidine <NUM> (Aldrich) is treated with sodium hydride and SEM chloride to afford <NUM>, which is reacted with commercially available <NUM> to provide <NUM>. Instead of <NUM>, <NUM>-bromo-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidine may also be used in the first step to provide the SEM-protected <NUM>-bromo-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidine (analogous to <NUM>) which can be reacted with <NUM> to provide <NUM>. Reaction of <NUM> with <NUM>, prepared as disclosed in Scheme 2a below, is performed in a manner analogous to that described in <NPL>, to give <NUM>. The reaction is performed in the presence of chiral ligand <NUM>, prepared as described in Lin, Q. <NUM> is converted to <NUM> by treatment with NH<NUM>OH and I<NUM>. The SEM protecting group of <NUM> is then deprotected with LiBF<NUM> and NH<NUM>OH to give compound <NUM>.

As shown in Scheme 2a, commercially available <NUM> is treated with phosphonium ylide <NUM> and DCl/D<NUM>O to provide <NUM>, which is treated with <NUM> and DiBAl-H to afford <NUM>. <CHM>
<CHM>.

Compounds analogous to <NUM> may also be prepared. For example, as shown in Scheme 2b, commercially available <NUM> may be converted to <NUM> in a manner analogous to that disclosed in Scheme 2a. As another example, as shown in Scheme 2c, commercially available <NUM> may be converted to <NUM> in a manner analogous to that disclosed in Scheme 2a and Scheme 2b. <NUM> may be converted, in a manner similar to that disclosed in Scheme <NUM>, to a compound of formula I wherein Y<NUM> and each Y<NUM> are deuterium and Y<NUM>, each Y<NUM>, each Y<NUM>, Y<NUM>, Y<NUM> and Y<NUM> are hydrogen. Likewise, <NUM> may be converted, in a manner similar to that disclosed in Scheme <NUM>, to a compound of formula I wherein Y<NUM> and each Y<NUM> are deuterium and Y<NUM>, each Y<NUM>, each Y<NUM>, Y<NUM>, Y<NUM> and Y<NUM> are hydrogen.

The specific approaches and compounds shown above are not intended to be limiting. The suitability of a chemical group in a compound structure for use in the synthesis of another compound is within the knowledge of one of ordinary skill in the art.

Additional methods of synthesizing the compound of Formula I and its synthetic precursors, including those within routes not explicitly shown in schemes herein, are within the means of chemists of ordinary skill in the art. Synthetic chemistry transformations and protecting group methodologies (protection and deprotection) useful in synthesizing the applicable compounds are known in the art and include, for example, those described in <NPL>); <NPL>); <NPL>); and <NPL>) and subsequent editions thereof.

The invention also provides pyrogen-free pharmaceutical compositions comprising an effective amount of the pharmaceutically acceptable salt of the compound of Formula I; and a pharmaceutically acceptable carrier. The carrier(s) are "acceptable" in the sense of being compatible with the other ingredients of the formulation and, in the case of a pharmaceutically acceptable carrier, not deleterious to the recipient thereof in an amount used in the medicament.

Pharmaceutically acceptable carriers, adjuvants and vehicles that may be used in the pharmaceutical compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat.

If required, the solubility and bioavailability of the compound of the present invention in pharmaceutical compositions may be enhanced by methods well-known in the art. One method includes the use of lipid excipients in the formulation. See "<NPL>; and "<NPL>.

Another known method of enhancing bioavailability is the use of an amorphous form of the compound of this invention optionally formulated with a poloxamer, such as LUTROL™ and PLURONIC™ (BASF Corporation), or block copolymers of ethylene oxide and propylene oxide. See <CIT>; and <CIT>and <CIT>.

The pharmaceutical compositions of the invention include those suitable for oral, rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration. In certain embodiments, the compound of the invention is administered transdermally (e.g., using a transdermal patch or iontophoretic techniques). Other formulations may conveniently be presented in unit dosage form, e.g., tablets, sustained release capsules, and in liposomes, and may be prepared by any methods well known in the art of pharmacy. See, for example, <NPL>).

Such preparative methods include the step of bringing into association with the molecule to be administered ingredients such as the carrier that constitutes one or more accessory ingredients. In general, the compositions are prepared by uniformly and intimately bringing into association the active ingredients with liquid carriers, liposomes or finely divided solid carriers, or both, and then, if necessary, shaping the product.

In certain embodiments, the compound is administered orally. Compositions of the present invention suitable for oral administration may be presented as discrete units such as capsules, sachets, or tablets each containing a predetermined amount of the active ingredient; a powder or granules; a solution or a suspension in an aqueous liquid or a non-aqueous liquid; an oil-in-water liquid emulsion; a water-in-oil liquid emulsion; packed in liposomes; or as a bolus, etc. Soft gelatin capsules can be useful for containing such suspensions, which may beneficially increase the rate of compound absorption.

In the case of tablets for oral use, carriers that are commonly used include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried cornstarch. When aqueous suspensions are administered orally, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening and/or flavoring and/or coloring agents may be added.

Compositions suitable for oral administration include lozenges comprising the ingredients in a flavored basis, usually sucrose and acacia or tragacanth; and pastilles comprising the active ingredient in an inert basis such as gelatin and glycerin, or sucrose and acacia.

Compositions suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampules and vials, and may be stored in a freeze dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets.

Such injection solutions may be in the form, for example, of a sterile injectable aqueous or oleaginous suspension. This suspension may be formulated according to techniques known in the art using suitable dispersing or wetting agents (such as, for example, Tween <NUM>) and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example, as a solution in <NUM>,<NUM>-butanediol. Among the acceptable vehicles and solvents that may be employed are mannitol, water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono- or diglycerides. Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant.

The pharmaceutical compositions of this invention may be administered in the form of suppositories for rectal administration. These compositions can be prepared by mixing the compound of this invention with a suitable non-irritating excipient which is solid at room temperature but liquid at the rectal temperature and therefore will melt in the rectum to release the active components. Such materials include, but are not limited to, cocoa butter, beeswax and polyethylene glycols.

The pharmaceutical compositions of this invention may be administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other solubilizing or dispersing agents known in the art. See, e.g.: <CIT>.

Topical administration of the pharmaceutical compositions of this invention is especially useful when the desired treatment involves areas or organs readily accessible by topical application. For topical application topically to the skin, the pharmaceutical composition should be formulated with a suitable ointment containing the active components suspended or dissolved in a carrier. Carriers for topical administration of the compound of this invention include, but are not limited to, mineral oil, liquid petroleum, white petroleum, propylene glycol, polyoxyethylene polyoxypropylene compound, emulsifying wax, and water. Alternatively, the pharmaceutical composition can be formulated with a suitable lotion or cream containing the active compound suspended or dissolved in a carrier. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate <NUM>, cetyl esters wax, cetearyl alcohol, <NUM>-octyldodecanol, benzyl alcohol, and water. The pharmaceutical compositions of this invention may also be topically applied to the lower intestinal tract by rectal suppository formulation or in a suitable enema formulation. Topically-transdermal patches and iontophoretic administration are also included in this invention.

Application of the subject therapeutics may be local, so as to be administered at the site of interest. Various techniques can be used for providing the subject compositions at the site of interest, such as injection, use of catheters, trocars, projectiles, pluronic gel, stents, sustained drug release polymers or other device which provides for internal access.

Thus, according to yet another embodiment, the compound of this invention may be incorporated into compositions for coating an implantable medical device, such as prostheses, artificial valves, vascular grafts, stents, or catheters. Suitable coatings and the general preparation of coated implantable devices are known in the art and are exemplified in <CIT>; <CIT>; and <CIT>. The coatings are typically biocompatible polymeric materials such as a hydrogel polymer, polymethyldisiloxane, polycaprolactone, polyethylene glycol, polylactic acid, ethylene vinyl acetate, and mixtures thereof. The coatings may optionally be further covered by a suitable topcoat of fluorosilicone, polysaccharides, polyethylene glycol, phospholipids or combinations thereof to impart controlled release characteristics in the composition. Coatings for invasive devices are to be included within the definition of pharmaceutically acceptable carrier, adjuvant or vehicle, as those terms are used herein.

According to another embodiment, the invention provides a method of coating an implantable medical device comprising the step of contacting said device with the coating composition described above. It will be obvious to those skilled in the art that the coating of the device will occur prior to implantation into a mammal.

According to another embodiment, the invention provides a method of impregnating an implantable drug release device comprising the step of contacting said drug release device with the compound of this invention or the composition of this invention. Implantable drug release devices include, but are not limited to, biodegradable polymer capsules or bullets, non-degradable, diffusible polymer capsules and biodegradable polymer wafers.

According to another embodiment, the invention provides an implantable medical device coated with the compound of this invention or a composition comprising the compound of this invention, such that said compound is therapeutically active.

According to another embodiment, the invention provides an implantable drug release device impregnated with or containing the compound of this invention or a composition comprising the compound of this invention, such that said compound is released from said device and is therapeutically active.

Where an organ or tissue is accessible because of removal from the subject, such organ or tissue may be bathed in a medium containing a composition of this invention, a composition of this invention may be painted onto the organ, or a composition of this invention may be applied in any other convenient way.

In another embodiment, a composition of this invention further comprises a second therapeutic agent. The second therapeutic agent may be selected from any compound or therapeutic agent known to have or that demonstrates advantageous properties when administered with a compound having the same mechanism of action as ruxolitinib. Such agents include those indicated as being useful in combination with ruxolitinib.

Preferably, the second therapeutic agent is an agent useful in the treatment or prevention of a disease or condition selected from myelofibrosis, including primary myelofibrosis, polycythemia vera, post-polycythemia vera myelofibrosis, chronic idiopathic myelofibrosis, post-essential thrombocythemia myelofibrosis, and essential thrombocythemia, pancreatic cancer, prostate cancer, breast cancer, leukemia, non-Hodgkin's lymphoma, multiple myeloma, psoriasis and alopecia areata.

In one embodiment, the second therapeutic agent is selected from lenalidomide, panobinostat, capecitabine, exemestane, and combinations thereof.

In another embodiment, the invention provides separate dosage forms of the compound of this invention and one or more of any of the above-described second therapeutic agents, wherein the compound and second therapeutic agent are associated with one another. The term "associated with one another" as used herein means that the separate dosage forms are packaged together or otherwise attached to one another such that it is readily apparent that the separate dosage forms are intended to be sold and administered together (within less than <NUM> hours of one another, consecutively or simultaneously).

In the pharmaceutical compositions of the invention, the compound of the present invention is present in an effective amount. As used herein, the term "effective amount" refers to an amount which, when administered in a proper dosing regimen, is sufficient to treat the target disorder.

The interrelationship of dosages for animals and humans (based on milligrams per meter squared of body surface) is described in <NPL>. Body surface area may be approximately determined from height and weight of the subject. See, e.g., <NPL>.

In one embodiment, an effective amount of the compound of this invention can range from <NUM> to <NUM>, such as <NUM> to <NUM>, such as <NUM> to <NUM>. Examples of ranges are from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, from <NUM> to <NUM>, and from <NUM> to <NUM>. In one embodiment, a dose of <NUM>, <NUM>, <NUM>, and <NUM> is administered once a day. In one embodiment a dose of <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> is administered twice a day.

Effective doses will also vary, as recognized by those skilled in the art, depending on the diseases treated, the severity of the disease, the route of administration, the sex, age and general health condition of the subject, excipient usage, the possibility of co-usage with other therapeutic treatments such as use of other agents and the judgment of the treating physician. For example, guidance for selecting an effective dose can be determined by reference to the prescribing information for ruxolitinib.

For pharmaceutical compositions that comprise a second therapeutic agent, an effective amount of the second therapeutic agent is between about <NUM>% and <NUM>% of the dosage normally utilized in a monotherapy regime using just that agent. Preferably, an effective amount is between about <NUM>% and <NUM>% of the normal monotherapeutic dose. The normal monotherapeutic dosages of these second therapeutic agents are well known in the art. See, e.g., <NPL>); <NPL>).

It is expected that some of the second therapeutic agents referenced above will act synergistically with the compound of this invention. When this occurs, it will allow the effective dosage of the second therapeutic agent and/or the compound of this invention to be reduced from that required in a monotherapy. This has the advantage of minimizing toxic side effects of either the second therapeutic agent of the compound of this invention, synergistic improvements in efficacy, improved ease of administration or use and/or reduced overall expense of compound preparation or formulation.

Disclosed herein is a method of inhibiting one or more of Janus Associated Kinases (JAKs) JAK1 and JAK2 in a cell, comprising contacting a cell with a pharmaceutically acceptable salt of the compound of Formula I.

Also disclosed herein is the pharmaceutically acceptable salt of this invention, or a composition of this invention, for use in a method of treating a disease that is beneficially treated by ruxolitinib in a subject in need thereof, said method comprising the step of administering to the subject an effective amount of the pharmaceutically acceptable salt of the compound of this invention or a composition of this invention. The subject may be a patient in need of such treatment. Such diseases are well known in the art and are disclosed in, but not limited to the following patent: <CIT>. Such diseases include, but are not limited to, diseases involving the immune system including, for example, organ transplant rejection (e.g., allograft refection and graft versus host disease); autoimmune diseases such as multiple sclerosis, rheumatoid arthritis, juvenile arthritis, type I diabetes, lupus, psoriasis, inflammatory bowel disease, ulcerative colitis, Crohn's disease, myasthenia gravis, immunoglobulin nephropathies, autoimmune thyroid disorders; allergic conditions such as asthma, food allergies, atopic dermatitis and rhinitis; viral diseases such as Epstein Barr virus (EBV), hepatitis B, hepatitis C, HIV, HTLV <NUM>, varicella-zoster virus (VZV) and human papilloma virus (HPV); skin disorders such as psoriasis (for example, psoriasis vulgaris), atopic dermatitis, skin rash, skin irritation, skin sensitization (e.g., contact dermatitis or allergic contact dermatitis; cancer, including those characterized by solid tumors (e.g., prostate cancer, renal cancer, hepatic cancer, pancreatic cancer, gastric cancer, breast cancer, lung cancer, cancers of the head and neck, thyroid cancer, glioblastoma, Kaposi's sarcoma, Castleman's disease, melanoma), hematological cancers (e.g., lymphoma, leukemia such as acute lymphoblastic leukemia, or multiple myeloma), and skin cancer such as cutaneous T-cell lymphoma (CTCL) and cutaneous B-cell lymphoma (examples of which include Sezary syndrome and mycosis fungoides; myeloproliferative disorders (MPDs) such as polycythemia vera (PV), essential thrombocythemia (ET), myeloid metaplasia with myelofibrosis (MMM), chronic myelomonocytic leukemia (CMML), hypereosinophilic syndrome (HES), systemic mast cell disease (SMCD); inflammation and inflammatory diseases, such as inflammatory diseases of the eye (e.g., iritis, uveitis, scleritis, conjunctivitis, or related disease), inflammatory diseases of the respiratory tract (e.g., the upper respiratory tract including the nose and sinuses such as rhinitis or sinusitis or the lower respiratory tract including bronchitis, chronic obstructive pulmonary disease, and the like), inflammatory myopathy such as myocarditis; systemic inflammatory response syndrome (SIRS) and septic shock; ischemia reperfusion injuries or a disease or condition related to an inflammatory ischemic event such as stroke or cardiac arrest; anorexia; cachexia; fatigue such as that resulting from or associated with cancer; restenosis; sclerodermitis; fibrosis; conditions associated with hypoxia or astrogliosis such as, for example diabetic retinopathy, cancer or neurodegeneration; gout; increased prostate size due to, e.g., benign prostatic hypertrophy or benign prostatic hyperplasia.

In one embodiment, the invention provides the pharmaceutically acceptable salt of this invention, or a composition of this invention, for use in a method of treating a disease or condition selected from myelofibrosis, including primary myelofibrosis, post-polycythemia vera myelofibrosis, post-essential thrombocythemia myelofibrosis, essential thrombocythemia or a combination thereof; pancreatic cancer; prostate cancer; breast cancer; leukemia; non-Hodgkin's lymphoma; multiple myeloma; psoriasis and a combination thereof in a subject in need thereof, said method comprising the step of administering to the subject an effective amount of the pharmaceutically acceptable salt of this invention or a composition of this invention.

In a particular embodiment, the pharmaceutically acceptable salt of this invention, or a composition of this invention, is for use in a method of treating a disease or condition selected from myelofibrosis, including primary myelofibrosis, post-polycythemia vera myelofibrosis and post-essential thrombocythemia myelofibrosis in a subject in need thereof.

Identifying a subject in need of such treatment can be in the judgment of a subject or a health care professional and can be subjective (e.g. opinion) or objective (e.g. measurable by a test or diagnostic method).

In another embodiment, any of the above methods of treatment comprises the further step of co-administering to the subject in need thereof one or more second therapeutic agents. The choice of second therapeutic agent may be made from any second therapeutic agent known to be useful for co-administration with ruxolitinib. The choice of second therapeutic agent is also dependent upon the particular disease or condition to be treated. Examples of second therapeutic agents that may be employed in the methods of this invention are those set forth above for use in combination compositions comprising a compound of this invention and a second therapeutic agent.

In particular, combination therapies may include co-administering the compound of Formula I and a second therapeutic agent to a subject in need thereof for treatment of the following conditions (with the particular second therapeutic agent indicated in parentheses following the indication: myelofibrosis (lenalidomide or panobinostat); pancreatic cancer (capecitabine); and breast cancer (exemestane).

The term "co-administered" as used herein means that the second therapeutic agent may be administered together with the compound of this invention as part of a single dosage form (such as a composition of this invention comprising the compound of the invention and a second therapeutic agent as described above) or as separate, multiple dosage forms. Alternatively, the additional agent may be administered prior to, consecutively with, or following the administration of the compound of this invention. In such combination therapy treatment, both the compound of this invention and the second therapeutic agent(s) are administered by conventional methods. The administration of a composition of this invention, comprising both the compound of the invention and a second therapeutic agent, to a subject does not preclude the separate administration of that same therapeutic agent, any other second therapeutic agent or the compound of this invention to said subject at another time during a course of treatment.

Effective amounts of these second therapeutic agents are well known to those skilled in the art and guidance for dosing may be found in patents and published patent applications referenced herein, as well as in <NPL>);<NPL>), and other medical texts. However, it is well within the skilled artisan's purview to determine the second therapeutic agent's optimal effective-amount range.

In one embodiment of the invention, where a second therapeutic agent is administered to a subject, the effective amount of the compound of this invention is less than its effective amount would be where the second therapeutic agent is not administered. In another embodiment, the effective amount of the second therapeutic agent is less than its effective amount would be where the compound of this invention is not administered. In this way, undesired side effects associated with high doses of either agent may be minimized. Other potential advantages (including without limitation improved dosing regimens and/or reduced drug cost) will be apparent to those of skill in the art.

Step <NUM>. Diethyl <NUM>,<NUM>,<NUM>,<NUM>-dg-cyclopentane-<NUM>,<NUM>-dicarboxylate (<NUM>). To a solution of diethyl malonate (<NUM>, <NUM> mmol) in ethanol (<NUM>) was added a <NUM> wt% solution of sodium ethoxide in ethanol (<NUM>, <NUM> mmol) followed by <NUM>,<NUM>,<NUM>,<NUM>-tetradeutero-<NUM>,<NUM>-dibromobutane (<NUM>, <NUM>, <NUM> mmol, CDN Isotopes, <NUM> atom %D). The resulting solution was stirred at reflux for two hours then cooled to room temperature and diluted with excess water. The majority of the ethanol was then removed via distillation and the resulting aqueous solution was extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with brine, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> as a yellow oil which was carried forward without purification. (<NUM>, <NUM>%).

Step <NUM>. <NUM>,<NUM>,<NUM>,<NUM>- d<NUM>-Cyclopentane-<NUM>-carboxylic acid(<NUM>). To a solution of <NUM> (<NUM>, <NUM> mmol) in ethanol (<NUM>) was added a <NUM> solution of sodium hydroxide (<NUM>). Additional water (<NUM>) was then added and the reaction stirred at reflux for three hours. Upon cooling to room temperature, the reaction was diluted with excess water and the majority of ethanol was removed via distillation. The aqueous solution was rendered acidic (pH<<NUM>) with 1N HCl and subsequently extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The resulting light orange solid was transfered to a pressure flask and water (<NUM>) was added. The pressure flask was sealed and the reaction stirred at <NUM> for <NUM> hours then was cooled to room temperature. The reaction was diluted with 1N HCl and extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as an amber oil which was used without purification.

Step <NUM>. <NUM>,<NUM>,<NUM>-d<NUM>-N-Methoxy-N-methylcyclopentanecarboxamide (<NUM>). <NUM> (<NUM>, <NUM> mmol) in acetonitrile (<NUM>) at <NUM> was added N,O-dimethylhydroxylamine hydrochloride (<NUM>, <NUM> mmol), TBTU (<NUM>, <NUM> mmol) and N,N-diisopropylethylamine (<NUM>, <NUM> mmol). The reaction stirred at room temperature for <NUM> hours, then was diluted with 1N HCl and extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with sat. NaHCO<NUM>, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The reulting product was purified by column chromatography (SiO<NUM>, <NUM>-<NUM>% ethyl acetate/hexanes) to afford <NUM> (<NUM>, <NUM>%) as a clear oil. MS (ESI) <NUM> [(M + H)+].

Step <NUM>. <NUM>,<NUM>,<NUM>,<NUM>- d<NUM>-Cyclopentane-<NUM>-carboxaldehyde(<NUM>). To a solution of <NUM> (<NUM>, <NUM> mmol) in THF (<NUM>) at <NUM> was added dropwise a <NUM> solution of LiAlH<NUM> in THF (<NUM>, <NUM> mmol). The reaction stirred at <NUM> for one hour then was quenched by sequential dropwise addition of water (<NUM>µL), <NUM>% NaOH (<NUM>µL) and water (<NUM>). The quenched reaction stirred at room temperature for <NUM> minutes then was filtered through Celite® and concentarted under reduced pressure. The resulting oil was diluted with 1N HCl and extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as a clear oil which was used without purification.

Step <NUM>. <NUM>-(<NUM>,<NUM>,<NUM>,<NUM>- d<NUM>-cyclopentyl)acrylonitrile (<NUM>). <NUM> (<NUM>, <NUM> mmol) was then added dropwise as a solution in THF (<NUM>). The reaction was stirred at room temperature for <NUM> hours then diluted with excess water and extracted with diethyl ether (<NUM> x <NUM>) and ethyl acetate (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, ><NUM>%) as a light orange oil which was used without purification.

Step <NUM>. (+/-)-(<NUM>-(<NUM>-(<NUM>-Cyano-<NUM>-(<NUM>,<NUM>,<NUM>-d<NUM>-cyclopentyl)ethyl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)methyl pivalate ((+/~)<NUM>). <NUM> (<NUM>, <NUM> mmol, preparation described in <NPL>) in acetonitrile (<NUM>) was added <NUM> (<NUM>, <NUM> mmol) followed by DBU (<NUM>µL, <NUM> mmol). The reaction stirred at room temperature for <NUM> hours then was concentrated under reduced vacuum. The resulting crude mixture was diluted with water and extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with 1N HCl, dried (Na<NUM>SO<NUM>), filtered and concentrated underreduced pressure. Purification via normal phase column chromatography (SiO<NUM>, <NUM>-<NUM>% ethyl acetate/hexanes) followed by reverse phase column chromatography (C18, <NUM>-<NUM>% acetonitrile/water containing <NUM>% formic acid) afforded (+/)<NUM> (<NUM>, <NUM>%) as a white foam. <NUM> NMR (DMSO-d<NUM>, <NUM>) δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (td, J = <NUM>, <NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> (s, <NUM>); MS (ESI) <NUM> [(M + H)+].

Step <NUM>. (R)-( <NUM>-(<NUM>-(<NUM>-cyano-<NUM>-(<NUM>,<NUM>,<NUM>-tetradeuterocyclopentyl)ethyl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)methyl pivalate ((R)-<NUM>). Racemic compound (+/-)<NUM> (<NUM>) was dissolved in acetonitrile at a concentration of <NUM>/mL and subjected to chiral separation by preparative HPLC on a Daicel ChiralPak AD column (<NUM> x <NUM>, <NUM>) with <NUM>µL of (+/-)<NUM> solution per injection using an isocratic method: <NUM>% isopropanol (+ <NUM>% diethylamine)/ <NUM>% hexane (+ <NUM>% diethylamine) at a flow rate of <NUM>/min. Under these conditions baseline separation was achieved with (S)-<NUM> eluting at <NUM> minutes and (R)-<NUM> eluting at <NUM> minutes. Fractions containing each enantiomer were pooled and concentrated yielding <NUM> of (S)-<NUM> as a colorless film and <NUM> of (R)-<NUM> as a colorless film.

Step <NUM>. (R)-<NUM>-(<NUM>-(<NUM>-Pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-(<NUM>,<NUM>,<NUM>,<NUM>-tetradeuterocyclopentyl)propanenitrile (Compound <NUM>). Compound (R)-<NUM> (<NUM>, <NUM> mmol, <NUM> equiv) was dissolved in methanol (<NUM>) in a <NUM> scintillation vial. Sodium hydroxide (<NUM> of a <NUM> solution, <NUM> mmol, <NUM> equiv) was added and the reaction was stirred at room temperature for <NUM> hours. The reaction was diluted with water (<NUM>) and brine (<NUM>). The aqueous mixture was extracted with ethyl acetate (<NUM> x <NUM>). The combined organic layers were washed with brine (<NUM>), dried over sodium sulfate, filtered, and evaporated. The crude material was purified using an Analogix automated chromatography system eluting with <NUM> to <NUM>% methanol in dichloromethane. Product fractions were pooled and evaporated yielding compound <NUM> as a white foam. The chiral purity was found to be ><NUM>% ee (Chiralpak OD <NUM> x <NUM>, <NUM>, <NUM> % (hexane + <NUM>% diethylamine) + <NUM>% (isopropanol + <NUM>% diethylamine), <NUM>/min, <NUM> retention time = <NUM>).

Step <NUM>. Diethyl <NUM>,<NUM>,<NUM>,<NUM>-d<NUM>-cyclopentane-<NUM>,<NUM>-dicarboxylate (<NUM>). <NUM>, <NUM>, <NUM> mmol, CDN Isotopes, <NUM> atom %D). The resulting solution was stirred at reflux for two hours then cooled to room temperature and diluted with excess water. The majority of the ethanol was then removed via distillation and the resulting aqueous solution was extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with brine, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> as a yellow oil which was carried forward without purification. (<NUM>, <NUM>%).

Step <NUM>. <NUM>,<NUM>,<NUM>,<NUM>-d<NUM>-Cyclopentane-<NUM>-carboxylic acid (<NUM>). <NUM> (<NUM>, <NUM> mmol) in ethanol (<NUM>) was added a <NUM> solution of sodium hydroxide (<NUM>). Additional water (<NUM>) was then added and the reaction stirred at reflux for three hours. Upon cooling to room temperature, the reaction was diluted with excess water and the majority of ethanol was removed via distillation. The aqueous solution was rendered acidic (pH<<NUM>) with 1N HCl and subsequently extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The resulting light orange solid was transfered to a pressure flask and water (<NUM>) was added. The pressure flask was sealed and the reaction stirred at <NUM> for <NUM> hours then was cooled to room temperature. The reaction was diluted with 1N HCl and extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as an amber oil which was used without purification.

Step <NUM>. <NUM>,<NUM>,<NUM>,<NUM>-d<NUM>-N-Methoxy-N-methylcyclopentanecarboxamide (<NUM>). <NUM> (<NUM>, <NUM> mmol) in acetonitrile (<NUM>) at <NUM> was added N,O-dimethylhydroxylamine hydrochloride (<NUM>, <NUM> mmol), TBTU (<NUM>, <NUM> mmol) and N,N-diisopropylethylamine (<NUM>, <NUM> mmol). The reaction stirred at room temperature for <NUM> hours, then was diluted with 1N HCl and extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with sat. NaHCO<NUM>, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The reulting product was purified by column chromatography (SiO<NUM>, <NUM>-<NUM>% acetone/hexanes) to afford <NUM> (<NUM>, <NUM>%) as a clear oil. MS (ESI) <NUM> [(M + H)+].

Step <NUM>. <NUM>,<NUM>,<NUM>,<NUM>- d<NUM>-Cyclopentane-<NUM>-carboxaldehyde(<NUM>). To a soultion of <NUM> (<NUM>, <NUM> mmol) in THF (<NUM>) at <NUM> was added dropwise a <NUM> solution of LiAlH<NUM> in THF (<NUM>, <NUM> mmol). The reaction stirred at room temperature for one hour then was quenched at <NUM> by sequential dropwise addition of water (<NUM>µL), <NUM>% NaOH (<NUM>µL) and water (<NUM>). The quenched reaction stirred at room temperature for <NUM> minutes then was filtered through Celite® and concentarted under reduced pressure. The resulting oil was diluted with 1N HCl and extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as a clear oil which was used without purification.

Step <NUM>. <NUM>-(<NUM>,<NUM>,<NUM>,<NUM>-d<NUM>-Cyclopeniyl)acrylonitrile (<NUM>). <NUM> (<NUM>, <NUM> mmol) was then added dropwise as a solution in THF (<NUM>). The reaction was stirred at room temperature for <NUM> hours then diluted with excess <NUM>:<NUM> water/brine and extracted with MTBE (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The resulting oil was dissolved in CH<NUM>Cl<NUM> (<NUM>) and washed with NaHSO<NUM> (<NUM> x <NUM>). The organic layer was dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as a light orange oil which was used without purification.

Step <NUM>. (+/-)-(<NUM>-(<NUM>-(<NUM>-Cyano-<NUM>-(<NUM>. <NUM>-d<NUM>-cyclopentyl)ethyl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)methyl pivalate ((+/-)<NUM>). <NUM> (<NUM>, <NUM> mmol, preparation described in <NPL>) in acetonitrile (<NUM>) was added <NUM> (<NUM>, <NUM> mmol) followed by DBU (<NUM>µL, <NUM> mmol). The reaction stirred at room temperature for <NUM> hours then was concentrated under reduced vacuum. The resulting crude mixture was diluted with water and extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with 1N HCl, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. Purification via normal phase column chromatography (SiO<NUM>, <NUM>-<NUM>% ethyl acetate/hexanes) afforded (+/-)<NUM> (<NUM>, <NUM>%) as a white foam. <NUM> NMR (DMSO-d<NUM>, <NUM>) δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (td, J = <NUM>, <NUM>, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> (q, J = <NUM>, <NUM>), <NUM> (dd, J = <NUM>, <NUM>, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> (s, <NUM>). ; MS (ESI) <NUM> [(M + H)+].

Step <NUM>. (R)-(<NUM>-(<NUM>-(<NUM>-Cyano-<NUM>-(<NUM>. <NUM>-d<NUM>-cyclopentyl)ethyl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)methyl pivalate ((R)-<NUM>). (+/-)<NUM> is achieved following the analytical method developed for the all protio analog (<NPL>). Single enantiomers of (+/-)<NUM> are obtained via chiral HPLC using a ChiralCel OD-H column (<NUM> x <NUM>, <NUM>) and a mobile phase of <NUM>% ethanol <NUM>% hexanes at a flow rate of <NUM>/min. The all protio (R) enantiomer has been shown to be the second eluting peak with a retention time of <NUM> minutes. The all protio (S)-enantiomer elutes first at <NUM> minutes. The deuterated analogs (R)-<NUM> and (S)-<NUM> are expected to have very similar retention times to the respective all protio enantiomers. Scaling this method to a semi-preparative ChiralCel OD-H allows for the separation of larger quantities of (R)-<NUM>.

Step <NUM>. (R)-<NUM>-(<NUM>-(<NUM>-Pyrrolo[<NUM>-d]pyrimidin-<NUM>-yl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-(<NUM>,<NUM>,<NUM>-d<NUM>-cyclopentyl)propanenitrile (Compound <NUM>). Treatment of (R)-<NUM> with aqueous sodium hydroxide in methanol in a manner analogous to the procedure described in <NPL> for the all protio analog affords Compound <NUM>.

Compound <NUM> may also be prepared from (+/-)<NUM> via (R)-<NUM> using substantially the same conditions disclosed above for the preparation of Compound <NUM> from (+/-)<NUM> via (R)-<NUM>.

Step <NUM>. Diethyl <NUM>,<NUM>,<NUM>,<NUM>,<NUM>,<NUM>,<NUM>,<NUM>-d<NUM>-Cyclopentane-<NUM>,<NUM>-dicarboxylate (<NUM>). <NUM>, <NUM>, <NUM> mmol, CDN Isotopes, <NUM> atom %D). The resulting solution was stirred at reflux for two hours then cooled to room temperature and diluted with excess water. The majority of the ethanol was then removed via distillation and the resulting aqueous solution was extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with brine, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> as a yellow oil (<NUM>, <NUM>%) which was carried forward without purification.

Step <NUM>. Perdeuterocyclopentane-<NUM>-carboxylic acid (<NUM>). To a solution of <NUM> (<NUM>, <NUM> mmol) in ethanol (<NUM>) was added a <NUM> solution of sodium hydroxide (<NUM>). Additional water (<NUM>) was then added and the reaction stirred at reflux for three hours. Upon cooling to room temperature, the reaction was diluted with excess water and the majority of ethanol was removed via distillation. The aqueous solution was rendered acidic (pH<<NUM>) with 1N HCl and subsequently extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The resulting light orange solid was transfered to a pressure flask and D<NUM>O (<NUM>) was added. The pressure flask was sealed and the reaction stirred at <NUM> for <NUM> hours then was cooled to room temperature. The reaction was diluted with 1N HCl and extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as a yellow oil which was used without purification.

Step <NUM>. N-Methoxy-N-methyl(cyclopentane-d<NUM>)carboxamide(<NUM>). To a solution of <NUM> (<NUM>, <NUM> mmol) in acetonitrile (<NUM>) at <NUM> was added N,O-dimethylhydroxylamine hydrochloride (<NUM>, <NUM> mmol), TBTU (<NUM>, <NUM> mmol) and N,N-diisopropylethylamine (<NUM>, <NUM> mmol). The reaction stirred at room temperature for <NUM> hours, then was diluted with 1N HCl and extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with sat. NaHCO<NUM>, dried (Na<NUM>SO<NUM>), filtered and concentrated under reduced pressure. The reulting product was purified by column chromatography (SiO<NUM>, <NUM>-<NUM>% ethyl acetate/hexanes) to afford <NUM> (<NUM>, <NUM>%) as a clear oil. MS (ESI) <NUM> [(M + H)+].

Step <NUM>. Perdeuterocyclopentane-<NUM>-carboxaldehyde (<NUM>). To a solution of <NUM> (<NUM>, <NUM> mmol) in THF (<NUM>) at <NUM> was added dropwise a <NUM> solution of LiAlH<NUM> in THF (<NUM>, <NUM> mmol). The reaction stirred at room temperature for one hour then was quenched at <NUM> by sequential dropwise addition of D<NUM>O (<NUM>), <NUM>% NaOD/D<NUM>O (<NUM>) and D<NUM>O (<NUM>). The quenched reaction stirred at room temperature for <NUM> minutes then was filtered through Celite® and concentrated under reduced pressure. The resulting oil was diluted with 1N DCl/D<NUM>O and extracted with diethyl ether (<NUM> x <NUM>). The organic layers were combined, dried (MgSO<NUM>), filtered and concentrated under reduced pressure to afford <NUM> (<NUM>, <NUM>%) as a clear oil which was used without purification.

Step <NUM>. <NUM>-(Perdeuterocyclopentyl)acrylonitrile (<NUM>). To a solution of diethyl cyanomethylphosphonate (<NUM>, <NUM> mmol) in THF (<NUM>) at <NUM> was added dropwise a <NUM> solution of potassium tert-butoxide in THF (<NUM>, <NUM> mmol). The reaction stirred at <NUM> for <NUM> hour. Aldehyde <NUM> (<NUM>, <NUM> mmol) was then added dropwise as a solution in THF (<NUM>). The reaction was stirred at room temperature for <NUM> hours then diluted with excess <NUM>:<NUM> water/brine and extracted with MTBE (<NUM> x <NUM>). The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated underreduced pressure. The organic layers were combined, dried (Na<NUM>SO<NUM>), filtered and concentrated underreduced pressure to afford <NUM> (<NUM>, <NUM>%) as a light orange oil which was used without purification.

Step <NUM>. (+/-)-(<NUM>-(<NUM>-(<NUM>-Cyano-<NUM>-(cyclopentyl-d<NUM>)ethyl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)methyl pivalate((+/-)<NUM>). To a solution of <NUM> (<NUM>, <NUM> mmol, preparation described in <NPL>) in acetonitrile (<NUM>) was added <NUM> (<NUM>, <NUM> mmol) followed by DBU (<NUM>µL, <NUM> mmol). The reaction stirred at room temperature for <NUM> hours then was concentrated under reduced vacuum. The resulting crude mixture was diluted with water and extracted with ethyl acetate (<NUM> x <NUM>). The organic layers were combined, washed with 1N HCl, dried (Na<NUM>SO<NUM>), filtered and concentrated underreduced pressure. Purification via normal phase column chromatography (SiO<NUM>, <NUM>-<NUM>% ethyl acetate/hexanes) afforded (+/-)<NUM> (<NUM>, <NUM>%) as a white foam. <NUM> NMR (DMSO-d<NUM>, <NUM>) δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (d, J = <NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (dd, J = <NUM>, <NUM>, <NUM>), <NUM> - <NUM> (m, <NUM>), <NUM> (s, <NUM>). ; MS (ESI) <NUM>[(M + H)+].

Step <NUM>. (R)-(<NUM>-(<NUM>-(<NUM>-Cyano-<NUM>-(cyclopentyl-d<NUM>)ethyl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)methyl pivalate ((R)-<NUM>). (+/-)<NUM> is achieved following the analytical method developed for the all protio analog (<NPL>). Single enantiomers of (+/-)<NUM> are obtained via chiral HPLC using a ChiralCel OD-H column (<NUM> x <NUM>, <NUM>) and a mobile phase of <NUM>% ethanol <NUM>% hexanes at a flow rate of <NUM>/min. The all protio (R) enantiomer has been shown to be the second eluting peak with a retention time of <NUM> minutes. The all protio (S)-enantiomer elutes first at <NUM> minutes. The deuterated analogs (R)-<NUM> and (S)-<NUM> are expected to have very similar retention times to the respective all protio enantiomers. Scaling this method to a semi-preparative ChiralCel OD-H allows for the separation of larger quantities of (R)-<NUM>.

Step <NUM>. (R)-<NUM>-(<NUM>-(<NUM>-Pyrrolo[<NUM>,<NUM>-d]pyrimidin-<NUM>-yl)-<NUM>-pyrazol-<NUM>-yl)-<NUM>-(cyclopentyl-d<NUM>)propanenitrile (Compound <NUM>). (R)-<NUM> with aqueous sodium hydroxide in methanol in a manner analogous to the procedure described in <NPL> for the all protio analog affords Compound <NUM>.

Microsomal Assay: Human liver microsomes (<NUM>/mL) are obtained from Xenotech, LLC (Lenexa, KS). β-nicotinamide adenine dinucleotide phosphate, reduced form (NADPH), magnesium chloride (MgCl<NUM>), and dimethyl sulfoxide (DMSO) are purchased from Sigma-Aldrich.

Determination of Metabolic Stability: <NUM> stock solutions of test compounds are prepared in DMSO. The <NUM> stock solutions are diluted to <NUM>-<NUM> in acetonitrile (ACN). The <NUM>/mL human liver microsomes are diluted to <NUM>/mL in <NUM> potassium phosphate buffer, pH <NUM>, containing <NUM> MgCl<NUM>. The diluted microsomes are added to wells of a <NUM>-well deep-well polypropylene plate in triplicate. A <NUM>µL aliquot of the <NUM>-<NUM> test compound is added to the microsomes and the mixture is pre-warmed for <NUM> minutes. Reactions are initiated by addition of pre-warmed NADPH solution. The final reaction volume is <NUM> and contains <NUM>/mL human liver microsomes, <NUM>-<NUM> test compound, and <NUM> NADPH in <NUM> potassium phosphate buffer, pH <NUM>, and <NUM> MgCl<NUM>. The reaction mixtures are incubated at <NUM>, and <NUM>µL aliquots are removed at <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> minutes and added to shallow-well <NUM>-well plates which contain <NUM>µL of ice-cold ACN with internal standard to stop the reactions. The plates are stored at <NUM> for <NUM> minutes after which <NUM>µL of water is added to the wells of the plate before centrifugation to pellet precipitated proteins. Supernatants are transferred to another <NUM>-well plate and analyzed for amounts of parent remaining by LC-MS/MS using an Applied Bio-systems API <NUM> mass spectrometer. The same procedure is followed for the non-deuterated counterpart of the compound of Formula I and the positive control, <NUM>-ethoxycoumarin (<NUM>). Testing is done in triplicate.

Data analysis: The in vitro t<NUM>/<NUM>s for test compounds are calculated from the slopes of the linear regression of % parent remaining (ln) vs incubation time relationship. <MAT> k = -[slope of linear regression of % parent remaining(ln) vs incubation time].

Data analysis is performed using Microsoft Excel Software.

Claim 1:
A pharmaceutically acceptable salt of a compound of Formula I:
<CHM>
wherein:
Y<NUM>, Y<NUM> and Y<NUM> are each hydrogen and the compound (Cmpd) is that set forth in the table below:

<TAB>

wherein when a position is designated specifically as "D" that position has at least <NUM>% incorporation of deuterium,
any atom not designated as "D" (deuterium) is present at its natural isotopic abundance,
and wherein the salt is selected from sulfate, pyrosulfate, bisulfate, sulfite, bisulfate, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caprate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, butyne-<NUM>,<NUM>-dioate, hexyne-<NUM>,<NUM>-dioate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, terephthalate, sulfonate, xylene sulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, <NUM>-hydroxybutyrate, glycolate, maleate, tartrate, methanesulfonate, propanesulfonate, naphthalene-<NUM>-sulfonate, naphthalene-<NUM>-sulfonate, and mandelate.