Patent Description:
Therapeutic agents that modulate or inhibit the activity of BET bromodomain-containing proteins such as BRD2, BRD3, BRD4, and BRDT have the potential to cure, treat, or improve the lives of patients suffering from diseases such as cancer, autoimmune, and cardiovascular diseases. In particular, BET bromodomain modulators or inhibitors have the potential to treat B-acute lymphocytic leukemia, Burkitt's lymphoma, Diffuse large cell lymphoma, Multiple myeloma, Primary plasma cell leukemia, Lung cancer, Bladder cancer, Breast cancer, Cervix cancer, Colon cancer, Gastric cancer, Glioblastoma, Prostate cancer, Ovarian cancer, and Neuroblastoma among others. Compounds for the treatment of such diseases and conditions are disclosed in <CIT>.

There is a large unmet need for compounds, including solid forms of derivatives of benzimidazole with high purity. Compounds that in addition to being efficacious also exhibit improved stability, solubility, and a pharmacokinetic and pharmacodynamics profile favorable for the treatment of diseases modulated by BET proteins containing bromodomains, and, importantly, can be made efficiently on a large scale to facilitate clinical and commercial use.

<CIT> describes substituted bicyclic compounds, which are useful for inhibition of BET protein function by binding to bromodomains.

The invention provides a process of preparing a solid crystalline form Compound I Form VII comprising the following steps:.

This and other embodiments of the present invention are set out in the appended claims.

The technical information set out below may in some respects go beyond the scope of the invention, which is defined by the appended claims. The additional technical information is provided to place the actual invention in a broader technical context and to illustrate possible related technical developments.

Any incidental references to methods for treatment of the human or animal body by surgery or therapy and diagnostic methods practiced on the human or animal body are not to be construed as claiming protection for such methods as such, but are instead to be construed as referring to products, in particular substances or compositions, for use in any of these methods.

Compound I is known to modulate or inhibit BET activity and is described in <CIT>. Compound I has the formula:
<CHM>.

The disclosure provides a solid form of Compound I, as well as methods for making the disclosed solid form of Compound I, intermediates used in its manufacture, pharmaceutical compositions comprising crystalline forms of Compound I, and methods for using such forms and pharmaceutical compositions in the treatment of diseases mediated by BET proteins. The details of one or more embodiments are set forth in the description below. Other features, objectives, and advantages will be apparent from the description and from the claims.

The disclosure provides a process of preparing Compound I that is suitable for scale up and manufacturing on large scale. A process of preparing Compound I is described in <CIT> , and we refer particularly to its description of Compound I and its synthesis. In comparison to the synthesis described in <CIT>, the process provided herein has certain advantages that make it suitable for scale-up of the preferred polymorphic form of Compound I (Form VII). For example, the process described herein uses less hazardous reagents, lower reaction temperatures, reagents better suited for scale-up, simplified work-up procedures, and streamlined isolation of intermediates by elimination of purification by column chromatography. All of these factors result in a manufacturing process that is better than prior methods in efficiency and quality, reduced environmental footprint, and reduced cost.

The invention provides a process of preparing a solid crystalline form Compound I Form VII, comprising the steps as set out in claim <NUM>.

In some embodiments, the process of preparing Compound I comprises starting materials A and G and intermediates B, C, D, E, and F:
<CHM>.

In some embodiments, the process of preparing Compound B comprises reacting Compound A (Step <NUM>):
<CHM>
with benzaldehyde in the presence of an acid. In some embodiments, the acid is acetic acid.

In some embodiments, step <NUM> is carried out in the presence of a solvent. In some embodiments, the solvent is methanol. In some embodiments, the solvent is ethanol. The use of methanol or ethanol as a solvent in step <NUM> provides a more efficient work up and isolation by eliminating the need for column chromatography.

In some embodiments of step <NUM>, NaHCO<NUM> solution is added to the reaction mixture after reaction completion, resulting in precipitation of the product, which simplifies the isolation and purification of Compound B.

In some embodiments of step <NUM>, Compound A and benzaldehyde are used in a <NUM>:<NUM> molar ratio.

In some embodiments, step <NUM> is conducted at a reduced temperature, and in some embodiments, the reaction temperature is <NUM>-<NUM>, or below <NUM>, or below <NUM>.

In some embodiments, the process of preparing Compound C comprises reacting Compound B (Step <NUM>):
<CHM>
with NaCNBH<NUM> or NaBH<NUM> in a solvent. In some embodiments, the solvent is an alcohol. In some embodiments, the solvent is methanol or ethanol.

In some embodiments, the solvent is ethanol, allowing for a reduction of the loading of NaCNBH<NUM> or NaBH<NUM> to <NUM> molar equivalents relative to Compound B, making the process more environmentally friendly and reducing the overall cost.

In some embodiments, the loading of NaCNBH<NUM> or NaBH<NUM> is equal to or less than one equivalent relative to Compound B, such as less than <NUM> equivalents, or less than <NUM> equivalents, or equal to or less than <NUM> equivalents relative to Compound B.

In some embodiments of Step <NUM>, the work-up and isolation is done by adding an HCl solution to quench the reaction followed by addition of water to precipitate the product (Compound C) in high purity (+<NUM>%), thereby simplifying the work-up and isolation process. In some embodiments, other agents can be used to quench the reaction, such as water or acetic acid.

In some embodiments, Step <NUM> is carried out at <NUM>-<NUM>, or <NUM>-<NUM>, or <NUM>-<NUM>.

The process of the invention comprises reacting Compound C with Compound G to produce Compound D.

In some embodiments, the process of preparing Compound D comprises reacting Compound C (Step <NUM>):
<CHM>
with
<CHM>
in the presence of a transition metal catalyst and a base, wherein the transition catalyst is a palladium catalyst.

In some embodiments, the palladium catalyst is [<NUM>,<NUM>'-bis(diphenylphosphino)ferrocene]dichloropalladium(II) (Pd(dppf)Cl<NUM>, dichloro(bis{di-tert-butyl[<NUM>-(dimethylamino)phenyl]phosphoranyl})palladium (PD-<NUM>), or tetrakis(tri(o-tolyl)phosphine)palladium(<NUM>).

In some embodiments the palladium catalyst is Pd(PPh<NUM>)<NUM>.

In some embodiments, the base is CsF. In some embodiments, the base is an alkali metal carbonate. In some embodiments, the base is K<NUM>CO<NUM>. In some embodiments, the base is an alkali metal phosphate. In some embodiments, the base is K<NUM>PO<NUM>.

The use of K<NUM>PO<NUM> is advantageous, compared to K<NUM>CO<NUM>, since K<NUM>PO<NUM> is well dispersed in the reactor and thus results in a reaction with less by-products, and the product (Compound D) of higher purity.

In some embodiments, the reaction is carried out in a solvent mixture comprising <NUM>,<NUM>-dioxane and water.

In some embodiments, the reaction is carried out at an elevated temperature, such as <NUM>-<NUM>, or <NUM>-<NUM>, or <NUM>-<NUM>.

The process of the invention comprises reacting Compound D with a first reagent to produce Compound E.

In some embodiments, the process of preparing Compound E comprises reacting Compound D (Step <NUM>):
<CHM>
with carbonyldiimidazole (CDI) in a solvent.

In some embodiments, the solvent is an aprotic solvent. In some embodiments the solvent is <NUM>,<NUM>-dioxane, DMSO, or DMF.

The advantage of using DMSO is that it is a safer (Class <NUM>) and more environmentally friendly solvent. The use of DMSO also allowed for the development of a simplified isolation procedure, involving precipitation of the product (Compound E) by addition of water to the reaction mixture. This eliminated a lengthy work-up process, including the use of chromatography, and thus provides Compound E in an efficient manner.

In some embodiments, CDI in a solvent is added potion wise to reduce the heat release during addition to the reaction mixture.

In some embodiments, the reaction is carried out at an elevated temperature, such as <NUM>-<NUM>, or <NUM>-<NUM>, or <NUM>-<NUM>, or <NUM>-<NUM>.

The process of the invention comprises reacting Compound E with a chlorination agent to produce Compound F.

In some embodiments, the process of preparing Compound F comprises reacting Compound E (step <NUM>):
<CHM>
with a chlorination reagent in the presence of a base.

In some embodiments, the chlorination reagent is phosphoryl chloride (POCl<NUM>).

In some embodiments, POCl<NUM> is used in excess (<NUM>-<NUM> molar equivalents relative to Compound E, or <NUM>-<NUM> equivalents, or <NUM>-<NUM> equivalents, or <NUM>-<NUM> equivalents).

In some embodiments, the base is an amine base.

In some embodiments, the amine base is N,N-diisopropylethylamine (DIPEA). In some embodiments, the amine base is trimethylamine or N, N-dimethylaniline.

In some embodiments, DIPEA was used in excess (<NUM>-<NUM> molar equivalents relative to Compound E, or <NUM> - <NUM> equivalents, or <NUM>-<NUM> equivalents).

In some embodiments, <NUM>-<NUM> molar equivalents, or <NUM>-<NUM> molar equivalents of POCl<NUM> relative to Compound E and <NUM>-<NUM> molar equivalents, or <NUM> molar equivalents of DIPEA are used relative to Compound E, reducing the formation of unwanted byproducts and simplifying the purification process of Compound E.

In some embodiments, step <NUM> is carried out at an elevated temperature such as <NUM>-<NUM> or <NUM>-<NUM>.

In some embodiments, Step <NUM> further comprises co-distillation of the crude reaction mixture with ethyl acetate. This is used as an efficient method to remove excess POCl<NUM> from the reaction mixture prior to quenching the reaction with NaHCO<NUM> solution.

In some embodiments, the product (Compound F) is isolated by crystallization from a mixture of ethyl acetate and non-polar co-solvent. In some embodiments the non-polar co-solvent is hexane, heptane, or toluene.

In some embodiments, the product (Compound F) is isolated by crystallization from an ethyl acetate/ n-heptane mixture.

In the invention, the process of preparing Compound I comprises reacting Compound F (Step <NUM>):
<CHM>
with methylamine. In some embodiments, methylamine <NUM> in THF is used.

In some embodiments, the reaction is carried out at <NUM>-<NUM>. This temperature avoids methylamine volatilization and pressure build-up.

In some embodiments, the reaction work-up comprises dissolving the crude product in an aqueous HCl solution (for example <NUM> N HCl) and washing with a non-polar solvent. In some embodiments, the non-polar solvent is dichloromethane, which efficiently removes remaining impurities from the previous step. Then, the crude product is neutralized with aqueous NaOH solution and the product is isolated.

As disclosed herein, the desired form (Form VII) of Compound I may be obtained from crystallization from ethanol (EtOH) and methyl tert-butyl ether (MTBE). Accordingly, the process of the invention comprises precipitating solid crystalline form Compound I Form VII from a solution comprising Compound I and ethanol, t-butylmethylether (MTBE), or a mixture thereof.

In some embodiments, to remove any remaining HCl, the dried Compound I can be dissolved in ethanol, treated with a solution of sodium hydroxide in ethanol, followed by addition of process water to precipitate the product.

In some embodiments, the crystallization was seeded with Compound I - Form VII to ensure formation of the desired polymorph.

In some embodiments, the precipitation is carried out by (<NUM>) cooling the ethanol solution of Compound I, (<NUM>) adding seed crystals of Compound I - Form VII, (<NUM>) stirring the mixture for about <NUM>, (<NUM>) cooling the mixture further and adding MTBE, (<NUM>) stirring for about <NUM>-<NUM> to precipitate Compound I - Form VII.

In some embodiments, MBTE can be added before the adding seed crystals of Compound I - Form VII.

Disclosed herein is a method of making a crystalline solid form of Compound I. Crystalline forms of the same compound typically have different properties, including hygroscopicity, solubility, and stability. Polymorphs with high melting points often have good thermodynamic stability which is advantageous in prolonging shelf-life for formulations containing the solid form of the compound. Polymorphs with lower melting points are typically less thermodynamically stable, but are favored by increased water solubility, often translating into increased bioavailability for the compound. Weakly hygroscopic polymorphs are often more stable to heat and humidity and resistant to degradation during storage. Anhydrous polymorphs are often favored as they can be consistently made without or with less variation in composition due to varying solvent and water content.

Compound I can be obtained in a solid crystalline form referred to as Form VII. Form VII is an anhydrate. Form VII, a polymorph of Compound I, is characterized by its XRPD and other data. As disclosed herein, Form VII of Compound I may be characterized by an X-ray powder diffractogram (XRPD) comprising a peak, in terms of <NUM>-theta, at <NUM> degrees ±<NUM> degrees θ, as determined on a diffractometer using a Cu-Kα radiation tube.

As disclosed herein, Form VII of Compound I may be characterized by an X-ray powder diffractogram (XRPD) comprising one or more peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determined on a diffractometer using a Cu-Kα radiation tube.

In some embodiments, Form VII of Compound I is characterized by an X-ray powder diffractogram (XRPD) comprising three or more peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determines on a diffractometer using a Cu-Kα radiation tube.

In some embodiments, Form VII of Compound I is characterized by an X-ray powder diffractogram (XRPD) comprising six or more peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determines on a diffractometer using a Cu-Kα radiation tube.

In some embodiments, Form VII of Compound I is characterized by an X-ray powder diffractogram (XRPD) comprising ten or more peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determines on a diffractometer using a Cu-Kα radiation tube.

In some embodiments, Form VII of Compound I is characterized by an X-ray powder diffractogram (XRPD) comprising peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determines on a diffractometer using a Cu-Kα radiation tube.

In some embodiments, Form VII of Compound I is characterized by an X-ray powder diffractogram (XRPD) pattern substantially as shown in <FIG>, as determines on a diffractometer using a Cu-Kα radiation tube.

In some embodiments, Form VII of Compound I is characterized by a differential scanning calorimetry (DSC) thermogram pattern with an endothermic peak at a temperature of about <NUM>.

In some embodiments, Form VII of Compound I is characterized by a differential scanning calorimetry (DSC) thermogram pattern substantially as shown in <FIG>.

In some embodiments, Form VII of Compound I is characterized by a thermogravimetric analysis (TGA) thermogram substantially as shown in <FIG>.

In some embodiments, Form VII of Compound I is characterized by an Infrared (IR) spectrum comprising IR bands at about <NUM>-<NUM> and <NUM>-<NUM>.

In some embodiments, Form VII of Compound I is characterized by an Infrared (IR) spectrum substantially as shown in <FIG>.

Also disclosed herein are intermediates useful in the synthesis of BET bromodomain inhibitors, such as Compound B:
<CHM>
and Compound C:
<CHM>.

Disclosed herein is a method of making a therapeutic agent (Compound I) that modulates or inhibits the activity of BET bromodomain-containing proteins such as BRD2, BRD3, BRD4, and BRDT, which has the potential to cure, treat, or improve the lives of patients suffering from diseases mediated by bromodomain-containing proteins, such as certain cancers, inflammatory diseases, and cardiovascular diseases.

A disease that is mediated, at least in part, by BET bromodomain-containing proteins in a subject in need thereof may be treated by a method
comprising administrating a therapeutically effective amount of Compound I in crystalline Form VII.

The disease may be selected from cancers, inflammatory diseases, and cardiovascular diseases.

The disease may be a cancer, including B-acute lymphocytic leukemia, Burkitt's lymphoma, diffuse large cell lymphoma, multiple myeloma, primary plasma cell leukemia, lung cancer, bladder cancer, breast cancer, cervical cancer, colon cancer, gastric cancer, glioblastoma, prostate cancer, ovarian cancer, and neuroblastoma.

The disease may be castration-resistant prostate cancer.

In one embodiment, the disease is metastatic castration-resistant prostate cancer.

The disease may be triple-negative breast cancer.

The disease may be estrogen-receptor positive breast cancer.

When treating a disease, at least in part, mediated by BET bromodomain-containing proteins in a subject in need thereof, the subject may benefit from combination drug treatment. For example, a form or forms of Compound I as described herein may be combined with one or more therapeutic agents in a single composition or in separately administered compositions that may be administered simultaneously, sequentially, or pursuant to a specified treatment regimen.

A form of Compound I as described herein may be administered sequentially with an additional therapeutic agent(s). Sequentially means that the for or forms of Compound I and the additional therapeutic agent(s) is (are) administered with a time separation of a few seconds (for example <NUM> sec. , <NUM> sec. , <NUM> sec. , <NUM> sec. or less), several minutes (for example <NUM>. or less, <NUM>. or less, <NUM>. or less), <NUM>-<NUM> hours, <NUM>-<NUM> days, or <NUM>-<NUM> weeks. When administered sequentially, the form or forms of Compound I as described herein and the additional therapeutic agent(s) may be administered in two or more administrations, and contained in separate compositions or dosage forms, which may be contained in the same or different package or packages.

A form or forms of Compound I as described herein may be combined with one or more therapeutic agent(s) used to treat cancer.

Compound I, having Form VII, as described herein, may be combined with one or more therapeutic agent(s) used to treat cancer.

Compound I, having Form VII, as described herein may be combined with a therapeutic agent selected from an androgen receptor antagonist, an androgen synthesis inhibitor, an aromatase inhibitor, a selective estrogen receptor modulator, a selective estrogen down-regulator, a poly ADP ribose polymerase (PARP) inhibitor, or an immunotherapeutic agent.

Compound I, having Form VII, as described herein may be combined with a therapeutic agent selected from Abiraterone (Zytiga), Enzalutamide (Xtandi), Apalutamide (ARN-<NUM>, Erleada), Darolutamide, Fulvestrant, Exemestane, Talazoparib, Olaperib, Veliparib, Rucaparib, Talazoparib, Niraparib, Pembrolizumab, Nivolumab, Durvalumab, and Rituximab.

The solid form of Form VII may have three or more characteristic XRPD peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determined on a diffractometer using a Cu-Kα radiation tube.

The solid form of Form VII may have six or more characteristic XRPD peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ , as determined on a diffractometer using a Cu-Kα radiation tube.

The solid form , such as anhydrate or Form VII, may have an XRPD pattern substantially similar to that shown in <FIG>.

Any of these solid forms may have a DSC thermogram pattern with an endothermic peak at a temperature of about <NUM>.

Also disclosed is a pharmaceutical composition comprising any of these solid forms and at least one pharmaceutically acceptable carrier.

Also disclosed is treating a cancer, comprising administrating to a subject in need of such treatment a therapeutically effective amount of any of these solid forms or pharmaceutical compositions. The cancer may be:.

Also disclosed is treating an inflammatory disease, comprising administrating to a patient in need of such treatment a therapeutically effective amount of any of these solid forms or pharmaceutical compositions.

Also disclosed is treating a cardiovascular disease, comprising administrating to a patient in need of such treatment a therapeutically effective amount of any of these solid forms or pharmaceutical compositions.

In the treatment of cancer, the subject may be a human.

Also disclosed is a process of preparing Form VII of Compound I:
<CHM>
comprising precipitating Form VII from a solution comprising Compound I and one or more solvents.

In said process of preparing Form VII of Compound I, the solvents may ethanol, t-butylmethylether (MTBE), or a mixture thereof.

In said process of preparing Form VII of Compound I, the precipitation may be carried out by.

optionally wherein MTBE is added before the adding the seed crystals of Compound I - Form VII to the mixture.

Also disclosed is a process of preparing Compound I
<CHM>
comprising steps (a)-(d):.

In some instances of said process of preparing Compound I:.

General methods: The FT-IR of compound I was obtained on a Nicolet <NUM> FT-IR spectrophotometer. The TGA analysis was conducted on a TA Q5000 instrument thermogravimetric analyzer. The DSC analysis was conducted on a TA Q2000 instrument differential scanning calorimeter from <NUM>-<NUM> at <NUM>/min. The XRPD pattern of Compound I was obtained on a Bruker instrument D8 advance diffractometer or similar, using mostly the following settings: <NUM> KV, <NUM> mA, Ka =<NUM> A (Cu-Ka radiation tube), scan scope <NUM>-<NUM> deg. <NUM> theta, <NUM> rpm, <NUM> deg. The NMR experiments were recorded on a Bruker AV400 MHz Spectrometer.

Compound A (<NUM>) was dissolved in methanol (<NUM>) and acetic acid (<NUM>) in a reactor. The solution was cooled to <NUM>-<NUM> and stirred for <NUM>-<NUM> hour, and benzaldehyde (<NUM>) was added dropwise over <NUM> hours. Once the reaction was complete (<NUM>-<NUM> hours), water (<NUM>) was added over <NUM> hours, and an NaHCO<NUM> solution (<NUM>% in water) was added dropwise over <NUM> hours, keeping the temperature low (<NUM>-<NUM>). The mixture was stirred for <NUM> hours. The solid was filtered off and washed with methanol/water <NUM>:<NUM>, followed by drying, leaving Compound B in <NUM>% yield and +<NUM>% purity by HPLC. <NUM>H-NMR (DMSO-d<NUM>): δ <NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>), <NUM>-<NUM> (<NUM>).

Compound B (<NUM>) was dissolved in ethanol (<NUM>) and NaBH<NUM> (<NUM>) was added in portions keeping the temperature between <NUM>-<NUM>. The reaction mixture was stirred for <NUM>-<NUM> until the reaction was complete as monitored by HPLC. An HCl solution (2N, <NUM>) was added, adjusting pH to <NUM>-<NUM>, followed by adding water (<NUM>) dropwise over <NUM> hours, keeping the temperature between <NUM>-<NUM>. The mixture was stirred for <NUM>-<NUM>, filtered and washed with an ethanol/water mixture (<NUM>:<NUM> ratio, <NUM>). The solid was dried at ~<NUM> for <NUM>-<NUM>, to afford Compound C (Yield: <NUM> (<NUM>%), Purity: <NUM>%). <NUM>H-NMR (DMSO-d<NUM>): δ <NUM>-<NUM> (<NUM>), <NUM> (<NUM>), <NUM>-<NUM> (<NUM>), <NUM> (<NUM>).

Compound C (<NUM>), Compound G (<NUM>), and potassium phosphate tribasic trihydrate (<NUM>) were mixed followed by addition of <NUM>,<NUM>-Dioxane (<NUM>) and water (<NUM>) and stirred for <NUM>-<NUM> hours. The resulting mixture was thoroughly purged with nitrogen. Tetrakis(triphenylphosphine)palladium(<NUM>) (<NUM>) was added, the solution thoroughly purged with nitrogen, and heated to <NUM>-<NUM> until the ratio of Compound C to Compound D was not more than <NUM>% measured by HPLC (<NUM>-<NUM> hours). After cooling to <NUM>-<NUM>, the reaction mixture was filtered, the solid washed with <NUM>,<NUM>-dioxane (<NUM>), the aqueous phase was removed, and the organic phase was concentrated. Water (<NUM>) was added dropwise at <NUM>-<NUM>, the mixture was cooled to <NUM>-<NUM>, and the mixture was stirred <NUM>-<NUM> hours until the amount of Compound D remaining in the mother liquors was not more than <NUM>%. Compound D was isolated by filtration and sequentially washed with <NUM>,<NUM>-dioxane/water (<NUM>:<NUM> ratio, <NUM>) and t-butylmethyl ether (<NUM>). The wet cake was mixed in methylene chloride (<NUM>) at <NUM>-<NUM> until all solids dissolved and silica gel (<NUM>) was added. After stirring for <NUM>-<NUM> hour, the mixture was cooled to <NUM>-<NUM>, filtered, washed with dicholomethane (<NUM>), then concentrated. The mixture was cooled and t-butylmethyl ether (<NUM>) was added dropwise at <NUM>-<NUM>. The reactor was cooled to <NUM>-<NUM> and stirre for <NUM>-<NUM> hours. The product was isolated by filtration, washed with methyl-t-butyl ether, and dried until the methylene chloride, t-butylmethyl ether, and moisture levels are not more than <NUM>%, to obtain Compound D (Yield: <NUM> (<NUM>%), Purity: <NUM>%). <NUM>H-NMR (DMSO-d<NUM>): δ <NUM>-<NUM> (<NUM>), <NUM>-<NUM> (<NUM>), <NUM> (<NUM>), <NUM>-<NUM> (<NUM>), <NUM>-<NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>). Alternatively, the wet cake obtained after washing sequentially with <NUM>,<NUM>-dioxane/water and MTBE can be purified using an ethanol (<NUM> vol. ) slurry at <NUM>-<NUM> for <NUM>-<NUM> hours, followed by drying until the moisture levels are not more than <NUM>% and the ethanol levels are not more than <NUM>%.

Carbonyldiimidazole solid (<NUM>) was added (CDI can be added portion wise) to a stirring mixture of Compound D (<NUM>) and dimethylsulfoxide (<NUM>). The mixture was heated to <NUM>-<NUM> for <NUM>-<NUM> hours or <NUM>-<NUM> until the ratio of Compound D to Compound E was NMT <NUM>%. The mixture was cooled to <NUM>-<NUM> and water was added over <NUM> hours. The resulting mixture was stirred at ambient temperature for <NUM>. The product was isolated by filtration and washed with water. The dimethylsulfoxide was verified to be NMT <NUM>% before drying using heat and vacuum. Drying was complete when the moisture level was NMT <NUM>%. Compound E was obtained Yield: <NUM>, <NUM>%, Purity: <NUM>%). <NUM>H-NMR (DMSO-d<NUM>): δ <NUM> (<NUM>), <NUM> (<NUM>), <NUM>-<NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>).

Compound E (<NUM>) and phosphorus oxychloride (<NUM>) were mixed and then treated with diisopropylethyl amine (DIPEA) (<NUM>) dropwise at <NUM>. The resulting mixture was heated to <NUM>-<NUM> for <NUM>-<NUM> hours. After the reaction was complete, the mixture was concentrated then cooled. Ethyl acetate was added and the mixture was concentrated under vacuum <NUM> times, co-distilling with ethyl acetate. Ethyl acetate (EtOAc) (<NUM>) was added to the concentrate, the mixture was cooled to ambient temperature, and then added to aqueous sodium bicarbonate (<NUM>%) slowly, keeping the micture <NUM>-<NUM>. The organic phase was separated and the organic layer was washed twice with aqueous sodium bicarbonate (<NUM>%) and then water. The organic phase was concentrated, ethyl acetate (<NUM>) was added, and the mixture was concentrated to assure that the moisture level was not more than <NUM>%. The mixture in ethyl acetate was decolorized with carbon (CUNO, contained <NUM> activated carbon). The mixture was concentrated and n-heptane (<NUM>) was added. The product was isolated by filtration and dried under vacuum. Drying was complete when residual moisture, ethyl acetate, and n-heptane were not more than <NUM>%. Compound F was obtained (<NUM>, <NUM>%, Purity: <NUM>%). <NUM>H-NMR (MeOH-d<NUM>): δ <NUM> (<NUM>), <NUM> (<NUM>), <NUM>-<NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>), <NUM> (<NUM>).

Compound F (<NUM>) was mixed with methylamine in tetrahydrofuran (THF) (<NUM>, <NUM>) and stirred at ambient temperature until the ratio of Compound F to Compound I was NMT <NUM>% by HPLC. After reaction completion, the mixture was concentrated under vacuum, water (<NUM>) was added dropwise to maintain ambient temperature, stirred for <NUM>-<NUM> hours, and the product isolated by filtration. The filter cake was washed with water. The wet cake was placed in a reactor and water (<NUM>) was added, and hydrochloric acid (<NUM>, <NUM>%) was added and stirred for <NUM> hours to dissolve the solids. The resulting solution was washed twice with methylene chloride to remove impurities. The aqueous solution was neutralized by adding a sodium hydroxide solution (<NUM>, <NUM>%) dropwise over <NUM> hours, and stirred for <NUM> hours. Compound I was isolated by filtration, washed with water, and dried under vacuum. If needed to remove any remaining hydrochloric acid, the dried material was dissolved in ethanol (<NUM>), treated with a solution of sodium hydroxide in ethanol (<NUM> NaOH in <NUM> ethanol), and stirred for <NUM> hour, followed by addition of <NUM> water to precipitate the product. Compound I was isolated by filtration, washed with water, and dried. If needed, Compound I may be further purified by a slurry in hot water.

Claim 1:
A process of preparing a solid crystalline form Compound I Form VII:
<CHM>
comprising the following steps:
(a) reacting
<CHM>
with Compound G:
<CHM>
to produce Compound D:
<CHM>
(b) reacting Compound D:
<CHM>
with a first reagent to produce Compound E:
<CHM>
(c) reacting Compound E:
<CHM>
with a chlorination agent to produce Compound F:
<CHM>
and
(d) reacting Compound F:
<CHM>
with methylamine, thereby providing Compound I; and
(e) precipitating solid crystalline form Compound I Form VII from a solution comprising Compound I and ethanol, t-butylmethylether (MTBE), or a mixture thereof, wherein the solid crystalline form Compound I Form VII has:
(i) three or more characteristic XRPD peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determined on a diffractometer using a Cu-Kα radiation tube; or
(ii) six or more characteristic XRPD peaks, in terms of <NUM>-theta, at <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, and <NUM> degrees, wherein each peak is ±<NUM> degrees θ, as determined on a diffractometer using a Cu-Ka radiation tube.