Patent Description:
Signal transducer and activator of transcription (STAT) proteins are transcription factors which transduce signals from various extracellular cytokines and growth factors to a nucleus. Seven (<NUM>) subtypes of STAT proteins (i.e., STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6) are currently known, and generally they consist of about <NUM> - <NUM> amino acids. In addition, each subtype of STAT proteins contains several conserved domains which play an important role in exhibiting the function of STAT proteins. Specifically, five (<NUM>) domains from N-terminus to C-terminus of STAT proteins have been reported including coiled-coiled domain, DNA binding domain, linker domain, SH2 domain and transactivation domain (TAD)). Further, X-ray crystalline structures of STAT1, STAT3, STAT4 and STAT5 have been reported since <NUM> (<NPL>; <NPL>; <NPL>; <NPL>). In general, receptors to which cytokines and growth factors bind are categorized into Class I and Class II. IL-<NUM>, IL-<NUM>, IL-<NUM>, IL-<NUM>, IL-<NUM>, G-CSF, GM-CSF, LIF, thrombopoietin, etc., bind to Class I receptors, while INF-α, INF-γ, IL-<NUM>, etc., bind to Class II receptors (<NPL>; <NPL>; <NPL>). Among them, the cytokine receptors involved in the activation of STAT proteins can be classified depending on their structural forms of extracellular domains into a gp-<NUM> family, an IL-<NUM> family, a growth factor family, an interferon family and a receptor tyrosine kinase family. Interleukin-<NUM> family cytokines are representative multifunctional cytokines which mediate various physiological activities. When interleukin-<NUM> cytokine binds to IL-<NUM> receptor which is present on the cell membrane surface, it attracts gp-<NUM> receptor to form an IL-<NUM>-gp-<NUM> receptor complex. At the same time, JAK kinases (JAK1, JAK2, JAK3 and Tyk2) in the cytoplasm are recruited to a cytoplasmic region ofgp130 to be phosphorylated and activated. Subsequently, latent cytoplasmic STAT proteins are attracted to a receptor, phosphorylated by JAK kinases and activated. Tyrosine-<NUM> adjacent to the SH2 domain located in the C-terminus of STAT proteins is phosphorylated, and the activated tyrosine-<NUM> of each STAT protein monomer binds to the SH2 domain of another monomer in a reciprocal manner, thereby forming a homo- or heterodimer. The dimers are translocalized into a nucleus and bind to a specific DNA binding promoter to promote the transcription. Through its transcription process, various proteins (Myc, Cyclin D1/D2, Bcl-xL, Mcl, survivin, VEGF, HIF-<NUM>, immune suppressors, etc.) associated with cell proliferation, survival, angiogenesis and immune evasion are produced (<NPL>; <NPL>).

In particular, STAT3 protein is known to play a crucial role in the acute inflammatory response and the signal transduction pathway of IL-<NUM> and EGF (<NPL>; <NPL>). According to the recent clinical report, STAT3 protein is constantly activated in patients with solid cancers occurring in prostate, stomach, breast, lung, pancreas, kidney, uterine, ovary, head and neck, etc., and also in patients with blood cancer such as acute and chronic leukemia, multiple myeloma, etc. Further, it has been reported that the survival rate of a patient group with activated STAT3 is remarkably lower than that of a patient group with inactivated STAT3 (<NPL>; <NPL>; <NPL>). Meanwhile, STAT3 was identified to be an essential factor for the growth and maintenance of murine embryonic stem cells in a study employing a STAT3 knockout mouse model. Also, a study with a tissue-specific STAT3-deficient mouse model reveals that STAT3 plays an important role in cell growth, apoptosis, and cell motility in a tissue-specific manner (<NPL>). Moreover, since apoptosis induced by anti-sensing STAT3 was observed in various cancer cell lines, STAT3 is considered as a promising new anticancer target. STAT3 is also considered as a potential target in the treatment of patients with diabetes, immune-related diseases, hepatitis C, macular degeneration, human papillomavirus infection, non-Hodgkin's lymphoma, tuberculosis, etc. Meanwhile, newly identified Th17 cells have been reported through a number of recent articles to be associated with various autoimmune diseases (<NPL>). Based on these reports, a control of the differentiation and function of Th17 cells is considered as a good target in the treatment of related diseases. In particular, since STAT3-dependent IL-<NUM> and IL-<NUM> signal transductions are known as important factors in the differentiation of Th17 cells (<NPL>; <NPL>), an inhibition of the function of STAT3 is expected to be effective in the treatment of diseases associated with Th17 cells such as systemic lupus erythematosus, uveitis, rheumatoid arthritis, autoimmune thyroid disease, inflammatory bowel disease, psoriasis and psoriatic arthritis (<NPL>).

Recently, IL-<NUM> and IL-<NUM> antibodies are under clinical studies on the treatment of arthritis and psoriasis associated with Th17 cells and exhibit a clinical efficacy (<NPL>; <NPL>). This also confirms that the inhibition of STAT3 signal transduction is an effective therapeutic method for such diseases.

In contrast, while having intracellular response pathways of identical cytokines and growth factors to those of STAT3, STAT1 increases inflammation and congenital and acquired immunities to inhibit the proliferation of cancer cells or cause pro-apoptotic responses, unlike STAT3 (<NPL>).

In order to develop STAT3 inhibitors, the following methods can be considered: i) inhibition of the phosphorylation of STAT3 protein by IL-<NUM>/gp-<NUM>/JAK kinase, ii) inhibition of the dimerization of activated STAT3 proteins, and iii) inhibition of the binding of STAT3 dimer to nuclear DNA. Small molecular STAT3 inhibitors are currently under development. Specifically, OPB-<NUM> and OPB-<NUM> are under clinical studies on patients with solid cancers or blood cancers by Otsuka Pharmaceutical Co. Further, S3I-<NUM> (<NPL>), S3I-M2001 (<NPL>), LLL-<NUM> (<NPL>), Stattic (<NPL>), STA-<NUM> (<NPL>), SF-<NUM>-<NUM> (<NPL>) and STX-<NUM> (<NPL>), etc. have been reported to be effective in a cancer cell growth inhibition experiment and in animal model (in vivo Xenograft model). Furthermore, although peptide compounds mimicking the sequence of amino acid of pY-<NUM> (STAT3) adjacent to the binding site to SH2 domain or the amino acid sequence of gp-<NUM> receptor in which JAK kinases bind were studied (<NPL>), the development of the peptide compounds has not been successful due to the problems such as solubility and membrane permeability.

<CIT> relates to compounds that bind to androgen receptors and/or modulate activity and/or amount of androgen receptors and methods for making and using such compounds.

Accordingly, it is an object of the present invention to provide novel heterocyclic derivatives for the inhibition of the activation of STAT3 protein.

It is another object of the present invention to provide uses of the heterocyclic derivatives for the prevention or treatment of diseases associated with the activation of STAT3 protein.

In accordance with one aspect of the present invention, there is provided a compound selected from the group consisting of a heterocyclic derivative represented by formula (I), and a pharmaceutically acceptable salt and a stereoisomer thereof:
<CHM>
wherein.

In accordance with another aspect of the present invention, there is provided a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof for use in preventing or treating diseases associated with the activation of STAT3 protein.

In accordance with a further aspect of the present invention, there is provided a pharmaceutical composition for use in preventing or treating diseases associated with the activation of STAT3 protein, comprising a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof as active ingredients.

The heterocyclic derivative represented by formula (I) above, or a pharmaceutically acceptable salt or a stereoisomer thereof has an excellent inhibitory effect on the activation of STAT3 protein, and thus it can be used for the prevention or treatment of diseases associated with the activation of STAT3 protein.

The present invention will be further described in detail herein below.

In the specification of the present invention, the term "halogen" refers to fluoro, chloro, bromo or iodo, unless specified otherwise.

The term "alkyl" refers to a linear or branched hydrocarbon moiety, unless specified otherwise.

The terms "haloalkyl", "haloalkoxy", "halophenyl", etc., respectively refer to alkyl, alkoxy, and phenyl substituted with at least one halogen.

The term "carbocycle" refers to an aromatic or non-aromatic hydrocarbon ring, which may be saturated or unsaturated, and a monocyclic or polycyclic radical. The term "carbocyclyl" refers to a radical of "carbocycle", and is used as a term inclusive of "cycloalkyl" and "aryl". The term "cycloalkyl" refers to a saturated hydrocarbon radical, which may be monocyclic or polycyclic. The term "aryl" refers to an aromatic hydrocarbon ring, which may be monocyclic or polycyclic.

The terms "carbocycle", "carbocyclyl", "cycloalkyl" and "aryl" may refer to, for example, a monocycle or polycycle having <NUM> to <NUM> carbon atoms, and will be indicated as "C<NUM>-<NUM> carbocycle", "C<NUM>-<NUM> carbocyclyl", "C<NUM>-<NUM> cycloalkyl", and "C<NUM>-<NUM> aryl", respectively.

The term "heterocycle" refers to an aromatic or non-aromatic ring having at least one heteroatom, which may be saturated or unsaturated, and a monocycle or polycycle. The term "heterocyclyl" refers to a radical of "heterocycle", which is used as a term inclusive of "heterocycloalkyl" and "heteroaryl". The term "heterocycloalkyl" refers to a saturated ring radical having at least one heteroatom, which may be monocyclic or polycyclic. The term "heteroaryl" refers to an aromatic ring radical having at least one heteroatom, which may be monocyclic or polycyclic.

The term "heteroatom" may be selected from N, O and S.

The terms "heterocycle", "heterocyclyl", "heterocycloalkyl" and "heteroaryl" may refer to, for example, a mono- or polycycle having <NUM> to <NUM> heteroatoms and/or carbon atoms, and will be indicated as "<NUM>- to <NUM>-membered heterocycle", "<NUM>- to <NUM>-membered heterocyclyl", "<NUM>- to <NUM>-membered heterocycloalkyl", and "<NUM>- to <NUM>-membered heteroaryl".

The terms "chain" refers to a saturated or unsaturated C<NUM>-<NUM> hydrocarbon chain not containing any heteroatoms in the chain, for example, ethylene, propylene, butylene and - CH<NUM>-CH=CH-; or a saturated or unsaturated C<NUM>-<NUM> hydrocarbon chain containing at least one heterogroup selected from the group consisting of -O-, -NH-, -N=, -S-, -S(=O)- and -S(=O)<NUM>-in the chain, for example, -CH<NUM>-O-CH<NUM>-, -CH<NUM>-O-CH<NUM>-O-CH<NUM>-, -CH<NUM>-CH=CH-NH- and - CH<NUM>-CH<NUM>-S(=O)<NUM>-CH<NUM>-O-, unless specified otherwise. The chain may be substituted with at least one selected from the group consisting of halogen, C<NUM>-<NUM>alkyl and C<NUM>-<NUM>alkoxy.

In a preferred embodiment of the compound of formula (I), RB is halogen, C<NUM>-<NUM>alkyl, C<NUM>-<NUM>alkoxy, haloC<NUM>-<NUM>alkyloxy, C<NUM>-<NUM>alkenyloxy, C<NUM>-<NUM>carbocyclyl-oxy, or <NUM>- to <NUM>-membered heterocyclyl-C<NUM>-<NUM>alkoxy.

In a preferred embodiment of the compound of formula (I),.

Preferable examples of the compound according to the present invention are listed below, and a pharmaceutically acceptable salt and a stereoisomer thereof are also included in the scope of the present invention:.

The above-listed names of the compounds are described in accordance with the nomenclature method provided by ChemBioDraw Ultra software (Version <NUM>. <NUM>) of PerkinElmer.

The present invention provides a pharmaceutically acceptable salt of a heterocyclic derivative represented by formula (I) above. The pharmaceutically acceptable salt should have low toxicity to humans, and should not have any negative impact on the biological activities and physicochemical properties of parent compounds. Examples of the pharmaceutically acceptable salt may include an acid addition salt between a pharmaceutically usable free acid and a basic compound represented by formula (I), an alkaline metal salt (sodium salt, etc.) and an alkaline earth metal salt (potassium salt, etc.), an organic base addition salt between an organic base and carboxylic acid represented by formula (I), amino acid addition salt, etc..

Examples of a suitable form of salts according to the present invention may be a salt with an inorganic acid or organic acid, wherein the inorganic acid may be hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, perchloric acid, bromic acid, etc., and the organic acid may be acetic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, fumaric acid, maleic acid, malonic acid, phthalic acid, succinic acid, lactic acid, citric acid, gluconic acid, tartaric acid, salicylic acid, malic acid, oxalic acid, benzoic acid, embonic acid, aspartic acid, glutamic acid, etc. The organic base which may be used for the preparation of the organic base addition salt may include tris(hydroxymethyl)methylamine, dicyclohexylamine, etc. Amino acids which may be used for the preparation of amino acid addition base may include natural amino acids such as alanine, and glycine.

The salts may be prepared using a conventional method. For example, the salts may be prepared by dissolving the compound represented by formula (I) in a water-miscible solvent such as methanol, ethanol, acetone, and <NUM>,<NUM>-dioxane, adding a free acid or a free base, and then crystallizing the resultant thereafter.

Additionally, the compounds of the present invention may have a chiral carbon center, and thus they may be present in the form of an R or S isomer, a racemic compound, an individual enantiomer or a mixture, an individual diastereomer or a mixture, and all these stereoisomers and a mixture thereof may belong to the scope of the present invention.

Additionally, the compounds of the present invention may also include a hydrate or solvate of the heterocyclic derivative represented by formula (I). The hydrate or solvate may be prepared using a known method, and they are preferred to be non-toxic and watersoluble, and in particular, they are preferably water or a hydrate or solvate having <NUM>-<NUM> molecules of alcoholic solvent (especially ethanol, etc.) bound thereto.

The present invention also provides a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof for use in preventing or treating diseases associated with the activation of STAT3 protein.

Further, the present invention provides a pharmaceutical composition for use in preventing or treating diseases associated with the activation of STAT3 protein, comprising a compound selected from the group consisting of a heterocyclic derivative represented by formula (I) above, and a pharmaceutically acceptable salt and a stereoisomer thereof as active ingredients.

Specifically, the diseases associated with the activation of STAT3 protein is selected from the group consisting of solid cancers, hematological or blood cancers, radio- or chemo-resistant cancers, metastatic cancers, inflammatory diseases, immunological diseases, diabetes, macular degeneration, human papillomavirus infection and tuberculosis.

More specifically, the diseases associated with the activation of STAT3 protein are selected from the group consisting of breast cancer, lung cancer, stomach cancer, prostate cancer, uterine cancer, ovarian cancer, kidney cancer, pancreatic cancer, liver cancer, colon cancer, skin cancer, head and neck cancer, thyroid cancer, osteosarcoma, acute or chronic leukemia, multiple myeloma, B- or T-cell lymphoma, non-Hodgkin's lymphoma, autoimmune diseases comprising rheumatoid arthritis, psoriasis, hepatitis, inflammatory bowel disease, Crohn's disease, diabetes, macular degeneration, human papillomavirus infection, and tuberculosis.

In particular, a heterocyclic derivative represented by formula (I) above, or a pharmaceutically acceptable salt or a stereoisomer thereof has an excellent inhibitory effect on the activation of STAT3 protein, and thus the present invention also provides a composition for the inhibition of STAT3 protein comprising the same as an active ingredient.

The pharmaceutical composition of the present invention, in addition to the heterocyclic derivative represented by formula (I) above, the pharmaceutically acceptable salt thereof, or the stereoisomer thereof, may further include as active ingredients, common and non-toxic pharmaceutically acceptable additives, for example, a carrier, an excipient, a diluent, an adjuvant, etc., to be formulated into a preparation according to a conventional method.

The pharmaceutical composition of the present invention may be formulated into various forms of preparations for oral administration such as tablets, pills, powders, capsules, syrups, or emulsions, or for parenteral administration such as intramuscular, intravenous or subcutaneous injections, etc., and preferably in the form of a preparation for oral administration.

Examples of the additives to be used in the pharmaceutical composition of the present invention may include sweeteners, binders, solvents, solubilization aids, wetting agents, emulsifiers, isotonic agents, absorbents, disintegrating agents, antioxidants, preservatives, lubricants, fillers, flavoring agents, etc. For example, they may include, lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, silica, talc, stearic acid, stearin, magnesium stearate, magnesium alluminosilicate, starch, gelatin, gum tragacanth, alginic acid, sodium alginate, methylcellulose, sodium carboxymethylcellulose, agar, water, ethanol, polyethylene glycol, polyvinylpyrrolidone, sodium chloride, calcium chloride, orange essence, strawberry essence, vanilla flavor, etc..

The pharmaceutical composition of the present invention may be formulated into a preparation for oral administration by adding additives to active ingredients, wherein the additives may include cellulose, calcium silicate, corn starch, lactose, sucrose, dextrose, calcium phosphate, stearic acid, magnesium stearate, calcium stearate, gelatin, talc, surfactants, suspension agents, emulsifiers, diluents, etc..

The pharmaceutical composition of the present invention may be formulated into a preparation for injection by adding additives to the active ingredients, for example, water, a saline solution, a glucose solution, an aqueous glucose solution analog, alcohol, glycol, ether, oil, fatty acid, fatty acid ester, glyceride, surfactants, suspension agents, emulsifiers, etc..

The compound of the present invention may be administered preferably in an amount ranging from <NUM> to <NUM>,<NUM>/day based on an adult subject with <NUM> body weight. The compound of the present invention may be administered once daily or a few divided doses. The dosage of the compound of the present invention may vary depending on the health conditions, age, body weight, sex of the subject, administration route, severity of illness, etc., and the scope of the present invention will not be limited to the dose suggested above.

Hereinafter, the present invention is described more specifically by the following examples, but these are provided only for illustration purposes and the present invention is not limited thereto.

The definition of the abbreviations used in the following examples is as follows.

<NUM>-Fluoro-<NUM>-methoxybenzaldehyde (<NUM>, <NUM> mmol) was dissolved in conc. H<NUM>SO<NUM> (<NUM>), and <NUM>% HNO<NUM> aqueous solution (<NUM>, <NUM> mmol) and conc. H<NUM>SO<NUM> (<NUM>, <NUM> mmol) were slowly added at -<NUM>. The reaction mixture was stirred at -<NUM> for <NUM> hours and poured into ice water. The precipitate was filtered and dissolved in CH<NUM>Cl<NUM> and neutralized with sat. NaHCO<NUM> aqueous solution. The organic extract was dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : CH<NUM>Cl<NUM> = <NUM> : <NUM>) to obtain <NUM>-fluoro-<NUM>-methoxy-<NUM>-nitrobenzaldehyde (<NUM>, <NUM>%) as a white solid. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (s, <NUM>).

<NUM>-Fluoro-<NUM>-methoxy-<NUM>-nitrobenzaldehyde (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF(<NUM>), and methyl <NUM>-mercaptoacetate (<NUM>µL, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added. The reaction mixture was stirred at <NUM> for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure to obtain methyl <NUM>-methoxy-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>) as a yellow solid without purification. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>).

Methyl <NUM>-methoxy-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in a mixture of MeOH/H<NUM>O (<NUM>, <NUM>/<NUM> v/v), and Zn (<NUM>, <NUM> mmol) and NH<NUM>Cl (<NUM>, <NUM> mmol) were added at room temperature. The reaction mixture was stirred at room temperature for <NUM> hours, filtered through Celite, and concentrated under reduced pressure. The residue was purified by flash column chromatography (amine silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain methyl-<NUM>-amino-<NUM>-methoxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as a yellow solid. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

Methyl <NUM>-amino-<NUM>-methoxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>), and <NUM> solution of BBr<NUM> in CH<NUM>Cl<NUM> (<NUM>, <NUM> mmol) was added at <NUM>. The reaction mixture was stirred at room temperature for <NUM> minutes, H<NUM>O was added, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-amino-<NUM>-hydroxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as a gray solid.

Methyl <NUM>-amino-<NUM>-hydroxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF (<NUM>), and <NUM>,<NUM>-dibromoethane (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added at room temperature. The reaction mixture was stirred at <NUM> for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM>%) as a gray solid.

Methyl <NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>), CH<NUM>SO<NUM>Cl (<NUM>µL, <NUM> mmol) was added dropwise at <NUM>. The reaction mixture was stirred at room temperature for <NUM> hour, H<NUM>O was added, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-g was repeated except for using methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM> mmol) to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylic acid (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-amino-<NUM>-hydroxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMA (<NUM>), and <NUM>-bromo-<NUM>-methyl propanoyl bromide (<NUM>µL, <NUM> mmol) and DIPEA (<NUM>µL, <NUM> mmol) were added. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-(<NUM>-bromo-<NUM>-methylpropanamido)-<NUM>-hydroxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-(<NUM>-bromo-<NUM>-methylpropanamido)-<NUM>-hydroxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMA(<NUM>) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) was added. The reaction mixture was stirred at <NUM> for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>,<NUM>-dimethyl-<NUM>-oxo-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM>%) as a gray solid.

Methyl <NUM>,<NUM>-dimethyl-<NUM>-oxo-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in THF (<NUM>), and <NUM> solution of BH<NUM>-THF complex (<NUM>, <NUM> mmol) was added at <NUM>. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>,<NUM>-dimethyl-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM>%) as a gray solid.

Methyl <NUM>,<NUM>-dimethyl-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in pyridine (<NUM>) and CH<NUM>SO<NUM>Cl (<NUM>µL, <NUM> mmol) was added dropwise at <NUM>. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>,<NUM>-dimethyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-g was repeated except for using methyl <NUM>,<NUM>-dimethyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylate (<NUM>, <NUM> mmol) as a starting material to obtain <NUM>,<NUM>-dimethyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylic acid (<NUM>, <NUM>%).

Methyl <NUM>-amino-<NUM>-hydroxybenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in pyridine (<NUM>), and CH<NUM>SO<NUM>Cl (<NUM>µL, <NUM> mmol) was added dropwise. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-hydroxy-<NUM>-(methylsulfonamido)benzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-hydroxy-<NUM>-(methylsulfonamido)benzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) as a starting material was dissolved in DMA (<NUM>µL), and <NUM>,<NUM>-dibromopropane (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazephine-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-g was repeated except for using methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazephine-<NUM>-carboxylate (<NUM>, <NUM> mmol) as a starting material to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazephine-<NUM>-carboxylic acid (<NUM>, <NUM>%).

Ethyl <NUM>-aminobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in pyridine (<NUM>), and <NUM>-methylbut-<NUM>-enoyl chloride (<NUM>µL, <NUM> mmol) was added. The reaction mixture was stirred at room temperature for <NUM> hour, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain ethyl <NUM>-(<NUM>-methylbut-<NUM>-enamido)benzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as a colorless liquid. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (q, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (t, <NUM>, J=<NUM>).

Ethyl <NUM>-(<NUM>-methylbut-<NUM>-enamido)benzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>), and AlCl<NUM> (<NUM>, <NUM> mmol) was added at <NUM>. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain ethyl <NUM>,<NUM>-dimethyl-<NUM>-oxo-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM>%) as a yellow solid. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (q, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (t, <NUM>, J=<NUM>).

The synthesis procedure of Intermediate <NUM>-c was repeated except for using ethyl <NUM>,<NUM>-dimethyl-<NUM>-oxo-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM> mmol) as a starting material to obtain ethyl <NUM>,<NUM>-dimethyl-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM>%). <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (q, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>).

The synthesis procedure of Intermediate <NUM>-f was repeated except for using ethyl <NUM>,<NUM>-dimethyl-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM> mmol) as a starting material to obtain ethyl <NUM>,<NUM>-dimethyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM>%). <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (q, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>).

The synthesis procedure of Intermediate <NUM>-g was repeated except for using ethyl <NUM>,<NUM>-dimethyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM> mmol) as a starting material to obtain <NUM>,<NUM>-dimethyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylic acid (<NUM>, <NUM>%).

<NUM>-bromo-<NUM>-fluorobenzaldehyde (<NUM>, <NUM> mmol) was dissolved in conc. H<NUM>SO<NUM> (<NUM>, <NUM> mmol), and <NUM>% HNO<NUM> (<NUM>, <NUM> mmol) was added at <NUM>. The reaction mixture was stirred for <NUM> hours, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was recrystallized using i-Pr<NUM>O to obtain <NUM>-bromo-<NUM>-fluoro-<NUM>-nitrobenzaldehyde (<NUM>, <NUM>%) as a white solid.

<NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>).

<NUM>-bromo-<NUM>-fluoro-<NUM>-nitrobenzaldehyde (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF (<NUM>), and methyl <NUM>-mercaptoacetate (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added, followed by heating at <NUM> for <NUM> hours. The reaction mixture was cooled to room temperature and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-bromo-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as a yellow solid. <NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>).

<NUM>-bromo-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMSO (<NUM>) and Cu<NUM>O (<NUM>, <NUM> mmol), sodium azide (<NUM>, <NUM> mmol) were added at room temperature. The mixture was stirred at <NUM> for <NUM> hour and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-amino-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%). as an off-white solid.

Methyl <NUM>-amino-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMA (<NUM>) and Boc<NUM>O (<NUM>, <NUM> mmol), DIPEA (<NUM>, <NUM> mmol) were added at room temperature. The mixture was stirred at <NUM> for <NUM> hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-((tert-butoxycarbonyl)amino)-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%), as an off-white solid. <NUM>H-NMR(<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>).

Methyl <NUM>-((tert-butoxycarbonyl)amino)-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in a mixture solvent of MeOH/H<NUM>O (<NUM>, <NUM>/<NUM> v/v), and Zn (<NUM>, <NUM> mmol) and NH<NUM>Cl (<NUM>, <NUM> mmol) were added thereto, and an ultrasonic reaction was conducted at <NUM> for <NUM> hours. The reaction mixture was filtered through Celite and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-amino-<NUM>-((tert-butoxycarbonyl) amino) benzo [b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%) as an off-white solid.

Methyl <NUM>-amino-<NUM>-((tert-butoxycarbonyl) amino) benzo [b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in pyridine (<NUM>), and methanesulfonyl chloride (<NUM>µL, <NUM> mmol) was slowly added thereto at <NUM>. The reaction mixture was warmed to room temperature, followed by stirred for <NUM> hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-((tert-butoxycarbonyl)amino)-<NUM>-(methylsulfonamido)benzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM>%), as an off-white solid.

Methyl <NUM>-((tert-butoxycarbonyl)amino)-<NUM>-(methylsulfonamido) benzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMA (<NUM>), and <NUM>,<NUM>-dibromoethane (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) were slowly added at room temperature. The mixture was stirred for <NUM> hour, and the reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-(tert-butyl) <NUM>-methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>,<NUM>(<NUM>H)-dicarboxylate (<NUM>, <NUM>%), as an off-white solid. <NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>).

<NUM>-(tert-butyl) <NUM>-methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>,<NUM>(<NUM>H)-dicarboxylate (<NUM>, <NUM> mmol) was dissolved in THF/H<NUM>O (<NUM>, <NUM>/<NUM> v/v), and LiOH·H<NUM>O (<NUM>, <NUM> mmol) was added. After stirring at room temperature for <NUM> hours, the reaction mixture was concentrated under a reduced pressure. The residue was diluted in H<NUM>O (<NUM>), and acidified with 1N HCl (pH <NUM>-<NUM>). The precipitate was filtered to obtain <NUM>-(tert-butoxycarbonyl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxylic acid (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-bromo-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF(<NUM>), and Pd(OAc)<NUM> (<NUM>, <NUM> mmol), PPh<NUM> (<NUM>, <NUM> mmol), TEA (<NUM>, <NUM> mmol) and methyl acrylate (<NUM>, <NUM> mmol) were added. The reaction mixture was stirred at <NUM> for <NUM> minutes, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was recrystallized with diethyl ether to obtain methyl (E)-<NUM>-(<NUM>-methoxy-<NUM>-oxoprop-<NUM>-en-<NUM>-yl)-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate as a white solid.

(E)-methyl <NUM>-(<NUM>-methoxy-<NUM>-oxoprop-<NUM>-en-<NUM>-yl)-<NUM>-nitrobenzo[b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in anhydrous MeOH(<NUM>), and <NUM>% Pd-C (<NUM>, <NUM> mmol) was added. The reaction mixture was charged with H<NUM> gas and stirred at <NUM> for <NUM> days. The residue was filtered through Celite and concentrated under reduced pressure to obtain methyl <NUM>-oxo-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid without purification.

Methyl <NUM>-oxo-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in THF (<NUM>), and <NUM> solution of BH<NUM>-THF complex (<NUM>, <NUM> mmol) was added at <NUM>. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM>%) as a gray solid.

Methyl <NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylatee (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>), and CH<NUM>SO<NUM>Cl (<NUM>µL, <NUM> mmol) and DIPEA (<NUM>µL, <NUM> mmol) were added at <NUM>. The reaction mixture was stirred for <NUM> hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-h was repeated except for using methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylate(<NUM>, <NUM> mmol) to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoline-<NUM>-carboxylic acid (<NUM>, <NUM> %), as a white solid. LC/MS ESI (+): <NUM> (M+<NUM>).

Unpurified tert-butyl <NUM>-(chlorocarbonyl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>(<NUM>H)-carboxylate (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM>/MeOH (<NUM>, <NUM>/<NUM> v/v), and TFA (<NUM>) was slowly added. After stirring at room temperature for <NUM> hours, the reaction mixture was concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in MeOH (<NUM>), and formaldehyde (<NUM>µL, <NUM> mmol) and sodium cyanoborohydride (<NUM>, <NUM> mmol) and AcOH (<NUM>µL, <NUM> mmol) were added thereto at room temperature. The mixture was stirred for <NUM> hours, and the reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated under a reduced pressure. The residue was separated on reversed-phase silica by column chromatography (<NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O = <NUM> : <NUM>), and fractions including the product were combined and evaporated to obtain methyl <NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in THF/H<NUM>O (<NUM>, <NUM>/<NUM> v/v), and LiOH·H<NUM>O (<NUM>, <NUM> mmol) was added thereto. After stirring at room temperature for <NUM> hours, the reaction mixture was concentrated under a reduced pressure. The residue was diluted in H<NUM>O (<NUM>), and acidified with 1N HCl (pH <NUM>-<NUM>). The precipitate was filtered to obtain <NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxylic acid (<NUM>, <NUM>%) as a white solid.

<NUM>-Chloro-<NUM>-methylphenol (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF (<NUM>), and <NUM>-chloro-<NUM>,<NUM>-diethoxypropane (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added. The reaction mixture was stirred at <NUM> for <NUM> hours, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain <NUM>-chloro-<NUM>-(<NUM>,<NUM>-diethoxypropoxy)-<NUM>-methylbenzene (<NUM>, <NUM>%) as a colorless liquid.

<NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (dd, <NUM>, J=<NUM>, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>).

Sodium methane sulfinate (<NUM>, <NUM> mmol) was dissolved in TFA(<NUM>) and stirred at <NUM> for <NUM> minutes. A solution of <NUM>-chloro-<NUM>-(<NUM>,<NUM>-diethxypropoxy)-<NUM>-methylbenzene (<NUM>, <NUM> mmol) in CH<NUM>Cl<NUM> (<NUM>) was added to the reaction mixture for a period of <NUM> hour, and stirred at room temperature for <NUM> minutes. The reaction mixture was basified with sat. NaHCO<NUM> aqueous solution (pH=<NUM>-<NUM>), and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex: EtOAc = <NUM> : <NUM>) to obtain <NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM>%) as an off-white solid.

<NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

<NUM>-Chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM> mmol) was dissolved in anhydrous <NUM>,<NUM>-dichloroethane (<NUM>), and N-bromosuccinimide (<NUM>, <NUM> mmol) and AIBN (<NUM>, <NUM> mmol) were added. The reaction mixture was refluxed at <NUM> for <NUM> hours, cooled to room temperature, H<NUM>O was added, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM>%) as a yellow solid.

<NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>-(Bromomethyl)-<NUM>-chloro-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM> mmol) was dissolved in anhydrous CH<NUM>CN (<NUM>), and <NUM>-methylmorpholine N-oxide (<NUM>, <NUM> mmol) and a molecular sieve (<NUM>) were added. The reaction mixture was stirred at room temperature for <NUM> hours, H<NUM>O was added to the reaction mixture, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was recrystallized with Et<NUM>O to obtain <NUM>-chloro-<NUM>-(methylsulfonyl)chromane-<NUM>-carbaldehyde (<NUM>, <NUM>%) as a pale yellow solid.

<NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

<NUM>-Chloro-<NUM>-(methylsulfonyl)chromane-<NUM>-carbaldehyde (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF (<NUM>), and methyl <NUM>-mercapto acetate (<NUM>µL, <NUM> mmol) and Cs<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added. The reaction mixture was stirred at <NUM> for <NUM> hours, cooled to room temperature, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was recrystallized with CH<NUM>Cl<NUM> and Et<NUM>O to obtain methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

<NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

Methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in THF (<NUM>)/H<NUM>O (<NUM>), and LiOH·H<NUM>O (<NUM>, <NUM> mmol) was added. The reaction mixture was stirred at <NUM> for <NUM> hours, 1N HCl (<NUM>) was added, and extracted with CH<NUM>Cl<NUM> and MeOH. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM>%) as an off-white solid.

<NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (bs, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

The synthesis procedure of Intermediate <NUM>-a was repeated except for using <NUM>-chloro-<NUM>-methylphenol (<NUM>, <NUM> mmol) to obtain <NUM>-chloro-<NUM>-(<NUM>,<NUM>-diethoxybutoxy)-<NUM>-methylbenzene (<NUM>, <NUM> %) as a colorless liquid.

The synthesis procedure of Intermediate <NUM>-b was repeated except for using <NUM>-chloro-<NUM>-(<NUM>,<NUM>-diethoxybutoxy)-<NUM>-methylbenzene (<NUM>, <NUM> mmol) to obtain <NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrobenzo[b]oxepine (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-c was repeated except for using <NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrobenzo[b]oxepine (<NUM>, <NUM> mmol) to obtain <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrobenzo[b]oxepine (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-d was repeated except for using <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrobenzo[b]oxepine (<NUM>, <NUM> mmol) to obtain <NUM>-chloro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrobenzo[b]oxepine-<NUM>-carbaldehyde (<NUM>, <NUM>%), as a white solid.

The synthesis procedure of Intermediate <NUM>-e was repeated except for using <NUM>-chloro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrobenzo[b]oxepine-<NUM>-carbaldehyde (<NUM>, <NUM> mmol) to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b]oxepine-<NUM>-carboxylic acid (<NUM>, <NUM> %) as a white solid.

To a solution of <NUM>-bromo-<NUM>-fluorobenzaldehyde (<NUM>, <NUM> mmol) in DMF (<NUM>) were added <NUM>-Ethylhexyl <NUM>-mercaptopropionate (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for 36hours. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-ethylhexyl <NUM>-((<NUM>-bromo-<NUM>-formylphenyl)thio)propanoate (<NUM>, <NUM> %) as a light brown oil.

A solution of <NUM>,<NUM>-diethoxybut-<NUM>-ene (<NUM>, <NUM> mmol) in <NUM>-borabicyclo(<NUM>. <NUM>)nonane (<NUM>, <NUM> mmol) was stirred for 1hour and concentrated. The residue was dissolved in benzene (<NUM>)/EtOH (<NUM>). 2N Na<NUM>CO<NUM> aqueous solution (<NUM>, <NUM> mmol) and Pd(PPh<NUM>)<NUM> (<NUM>, <NUM> mmol) were added and the reaction mixture at <NUM> for 90minutes. The reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated. The residue was purified by normal phase column chromatography (EtOAc:n-Hex=<NUM>:<NUM>) to give <NUM>-ethylhexyl <NUM>-((<NUM>-(<NUM>,<NUM>-diethoxybutyl)-<NUM>-formylphenyl)thio)propanoate (<NUM>, <NUM> %) as crude. To a solution of <NUM>-ethylhexyl <NUM>-((<NUM>-(<NUM>,<NUM>-diethoxybutyl)-<NUM>-formylphenyl)thio)propanoate (<NUM>, <NUM> mmol) in EtOH (<NUM>) was added NaBH<NUM> (<NUM>, <NUM> mmol). The reaction mixture was stirred for <NUM> minutes. NH<NUM>Cl was added, and the resulting mixture was stirred for <NUM> minutes and filtered through celite. The filtrate was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, and concentrated. The residue was purified by normal phase column chromatography (EtOAc:n-Hex=<NUM>:<NUM>) to give <NUM>-ethylhexyl <NUM>-((<NUM>-(<NUM>,<NUM>-diethoxybutyl)-<NUM>-(hydroxymethyl)phenyl)thio)propanoate (<NUM>, <NUM> %) as a colorless oil. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM> ), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (brs, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>).

Sodium methanesulfinate (<NUM>, <NUM> mmol) was dissolved in TFA (<NUM>) and the reaction mixture was stirred for <NUM> minutes. After cooling to <NUM>, a solution of <NUM>-ethylhexyl <NUM>-((<NUM>-(<NUM>,<NUM>-diethoxybutyl)-<NUM>-(hydroxymethyl)phenyl)thio)propanoate (<NUM>, <NUM> mmol) in CH<NUM>Cl<NUM> (<NUM>µl) was added dropwise and the reaction mixture was stirred at <NUM> for 1hour. The reaction mixture was extracted with EtOAc, and the organic extract was washed with sat. NaHCO<NUM> aqueous solution and brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-((<NUM>-(methylsulfonyl)-<NUM>-((<NUM>,<NUM>,<NUM>-trifluoroacetoxy)methyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalen-<NUM>-yl)thio)propanoate (<NUM>, <NUM> %) as a colorless oil. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (q, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>).

To a solution of <NUM>-ethylhexyl <NUM>-((<NUM>-(methylsulfonyl)-<NUM>-((<NUM>,<NUM>,<NUM>-trifluoroacetoxy)methyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalen-<NUM>-yl)thio)propanoate (<NUM>, <NUM> mmol) in DMF (<NUM>µl) was added <NUM> solution of potassium-t-butoxide in THF (<NUM>µl, <NUM> mmol) at -<NUM>~-<NUM>. The reaction mixture was stirred for 10minutes. tert-butyl <NUM>-bromoacetate (<NUM>µl, <NUM> mmol) was added at -<NUM>~-<NUM> and the reaction mixture was stirred at room temperature for 1hour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain tert-butyl <NUM>-((<NUM>-(hydroxymethyl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalen-<NUM>-yl)thio)acetate (<NUM>, <NUM> %) as a white amorphous. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (q, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

To a solution of tert-butyl <NUM>-((<NUM>-(hydroxymethyl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalen-<NUM>-yl)thio)acetate (<NUM>, <NUM> mmol) in CH<NUM>Cl<NUM> (<NUM>µl) was added Dess-Martin periodinane (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for 1hour. The reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated to give tert-butyl <NUM>-((<NUM>-formyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalen-<NUM>-yl)thio)acetate (<NUM>, <NUM> %) as crude. The crude was used the next step without purification. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

To a solution of tert-butyl <NUM>-((<NUM>-formyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalen-<NUM>-yl)thio)acetate (<NUM>, <NUM> mmol) in DMF (<NUM>µl) was added Cs<NUM>CO<NUM> (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> hour. The reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated to give tert-butyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphtho[<NUM>,<NUM>-b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> %) as crude. The crude was used the next step without purification. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

To a solution of tert-butyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphtho[<NUM>,<NUM>-b]thiophene-<NUM>-carboxylate (<NUM>, <NUM> mmol) in CH<NUM>Cl<NUM> (<NUM>) was added TFA (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for 30minutes. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphtho[<NUM>,<NUM>-b]thiophene-<NUM>-carboxylic acid (<NUM>, <NUM> %) as a white amorphous.

To a suspension of methyl <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) in THF (<NUM>)/CH<NUM>CN (<NUM>) was added NaH (<NUM>, <NUM> mmol). After 1hour, CH<NUM>I (<NUM>, <NUM> mmol) was added and the reaction mixture was stirred for 3days. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> %) as an off-white amorphous.

To a suspension of methyl <NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) in THF (<NUM>µl)/H<NUM>O (<NUM>µl) was added LiOHH<NUM>O (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> hour. The reaction mixture was concentrated and the residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM> %) as a white amorphous.

<NUM>-Bromo-<NUM>-methylbenzoate (<NUM>, <NUM> mmol) was dissolved in anhydrous <NUM>,<NUM>-dichloroethane (<NUM>), and N-bromosuccinimide (<NUM>, <NUM> mmol) and AIBN (<NUM>, <NUM> mmol) were added at room temperature. The mixture was refluxed at <NUM> for <NUM> hour, followed by cooling to room temperature and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-bromo-<NUM>-(bromomethyl)benzoate (<NUM>, <NUM>%) as a colorless liquid.

Glycolaldehyde diethyl acetal (<NUM>, <NUM> mmol) was dissolved in anhydrous THF (<NUM>), and NaH (<NUM>, <NUM> mmol) was added at <NUM>. The mixture was stirred at <NUM> for <NUM> minutes. Methyl <NUM>-bromo-<NUM>-(bromomethyl)benzoate (<NUM>, <NUM> mmol) in THF (<NUM>) was added at <NUM>. The mixture was stirred at <NUM> for <NUM> hours and extracted with EtOAc. The organic extract was washed with water, dried over anhydrous Na<NUM>SO<NUM>, and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain methyl <NUM>-bromo-<NUM>-((<NUM>,<NUM>-diethoxyethoxy)methyl)benzoate (<NUM>, <NUM>%) as a colorless liquid. <NUM>H-NMR(<NUM>, CDCl<NUM>): δ <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (t, <NUM>, J=<NUM>, <NUM>).

Methyl <NUM>-bromo-<NUM>-((<NUM>,<NUM>-diethoxyethoxy)methyl)benzoate (<NUM>, <NUM> mmol) was dissolved in DMF (<NUM>), and sodium methanethiolate (<NUM>, <NUM> mmol) was added at room temperature. The mixture was stirred at <NUM> for <NUM> hours, followed by cooling to room temperature and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-((<NUM>,<NUM>-diethoxyethoxy)methyl)-<NUM>-(methylthio)benzoic acid (<NUM>, <NUM>%) as a colorless liquid.

<NUM>-((<NUM>,<NUM>-diethoxyethoxy)methyl)-<NUM>-(methylthio)benzoic acid (<NUM>, <NUM> mmol) was dissolved in THF (<NUM>), and <NUM> solution of LiAlH4 in THF (<NUM>, <NUM> mmol) was added at room temperature. The mixture was stirred at room temperature for <NUM> hour and H<NUM>O was added at <NUM>. The reaction mixture was filtered through Celite and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain (<NUM>-((<NUM>,<NUM>-diethoxyethoxy)methyl)-<NUM>-(methylthio)phenyl)methanol (<NUM>, <NUM>%) as a colorless liquid. <NUM>H-NMR(<NUM>, CDCl<NUM>): δ <NUM> (d, <NUM>, J=<NUM>), <NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (brs, <NUM>), <NUM> (t, <NUM>, J=<NUM>, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM> (brs, <NUM>), <NUM> (t, <NUM>, J=<NUM>, <NUM>).

Sodium methanesulfinate (<NUM>, <NUM> mmol) was dissolved in TFA (<NUM>) and stirred at room temperature for <NUM> minutes. (<NUM>-((<NUM>,<NUM>-diethoxyethoxy)methyl)-<NUM>-(methylthio)phenyl)methanol (<NUM>, <NUM> mmol) in CH<NUM>Cl<NUM> (<NUM>) was slowly added at room temperature. The mixture was stirred at room temperature for <NUM> minutes and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under a reduced pressure. The mixture was dissolved in THF (<NUM>)/MeOH (<NUM>)/H<NUM>O (<NUM>) and LiOHH<NUM>O (<NUM>, <NUM> mmol) was added at room temperature. The mixture was stirred at room temperature for <NUM> minutes and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated to obtain (<NUM>-(methylsulfonyl)-<NUM>-(methylthio)isochroman-<NUM>-yl)methanol (<NUM>, <NUM>%)%) as a colorless liquid.

(<NUM>-(Methylsulfonyl)-<NUM>-(methylthio)isochroman-<NUM>-yl)methanol (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>) and Dess-Martin Periodinane (<NUM>, <NUM> mmol) was added at room temperature. The mixture was stirred at room temperature for <NUM> minutes and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain <NUM>-(methylsulfonyl)-<NUM>-(methylthio)isochromane-<NUM>-carbaldehyde (<NUM>, <NUM>%) as a colorless liquid.

A mixture of <NUM>-(Methylsulfonyl)-<NUM>-(methylthio)isochroman-<NUM>-carbaldehyde (<NUM>, <NUM> mmol), magnesium oxide (<NUM>, <NUM> mmol) and <NUM>-chloroacetic acid (<NUM>, <NUM> mmol) was stirred at <NUM> for <NUM> hours and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]isochromene-<NUM>-carboxylic acid (<NUM>, <NUM>%) as a white solid.

<NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM> mmol) was dissolved in THF (<NUM>), and LDA(<NUM>, <NUM> mmol) was slowly added at -<NUM> and stirred for <NUM> hour. NFS (<NUM>, <NUM> mmol) was added, followed by stirring at -<NUM> for <NUM> hours. The reaction was quenched with H<NUM>O, and the reaction mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain <NUM>-chloro-<NUM>-fluoro-<NUM>-methyl-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM>%) as a a white solid
<NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>).

The synthesis procedure of Intermediate <NUM>-c was repeated except for using <NUM>-chloro-<NUM>-fluoro-<NUM>-methyl-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM> mmol) to obtain <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>-fluoro-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM>%). <NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (q, <NUM>, J=<NUM>, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

The synthesis procedure of Intermediate <NUM>-d was repeated except for using <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>-fluoro-<NUM>-(methylsulfonyl)chromane (<NUM>, <NUM> mmol) to obtain <NUM>-chloro-<NUM>-fluoro-<NUM>-(methylsulfonyl)chromane-<NUM>-carbaldehyde (<NUM>, <NUM>%).

<NUM>-chloro-<NUM>-fluoro-<NUM>-(methylsulfonyl)chromane-<NUM>-carbaldehyde (<NUM>, <NUM> mmol) was dissolved in anhydrous DMF (<NUM>), and methyl <NUM>-mercaptoacetate (<NUM>, <NUM> mmol) and Cs<NUM>CO<NUM> (<NUM>, <NUM> mmol) were added, followed by heating at <NUM> for <NUM> hours. The reaction mixture was cooled to room temperature and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-fluoro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM>%) as a yellow solid.

To a solution of <NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene (<NUM>, <NUM> mmol) in acetic anhydride (<NUM>µl, <NUM> mmol) was added chromium oxide(VI) (<NUM>, <NUM> mmol) at <NUM>. The reaction mixture was stirred at <NUM> for 2hours. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydronaphthalen-<NUM>(<NUM>H)-one (<NUM>, <NUM> %) as a white amorphous.

A suspension of <NUM>-chloro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydronaphthalen-<NUM>(<NUM>H)-one (<NUM>, <NUM> mmol) in DAST (<NUM>, <NUM> mmol) was stirred at <NUM> overnight. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-chloro-<NUM>,<NUM>-difluoro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene (<NUM>, <NUM> %) as a white amorphous.

To a solution of <NUM>-chloro-<NUM>,<NUM>-difluoro-<NUM>-methyl-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene (<NUM>, <NUM> mmol) and N-bromosuccinimide (<NUM>, <NUM> mmol) in <NUM>,<NUM>-dichloroethane (<NUM>µl) was added AIBN (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> hour. The reaction mixture was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>,<NUM>-difluoro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene (<NUM>, <NUM> %) as a white amorphous.

A suspension of <NUM>-(bromomethyl)-<NUM>-chloro-<NUM>,<NUM>-difluoro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene (<NUM>, <NUM> mmol), N-methyl morpholine-N-oxide (<NUM>, <NUM> mmol) and molecular sieve (4Å) in CH<NUM>CN (<NUM>) was stirred at <NUM> for <NUM> hour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-chloro-<NUM>,<NUM>-difluoro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene-<NUM>-carbaldehyde (<NUM>, <NUM> %) as a light brown amorphous.

To a solution of <NUM>-chloro-<NUM>,<NUM>-difluoro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphthalene-<NUM>-carbaldehyde (<NUM>, <NUM> mmol) and Cs<NUM>CO<NUM> (<NUM>, <NUM> mmol) in DMF (<NUM>µl) was added methyl thioglycollate (<NUM>µl, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> hour. The reaction mixture was cooled to <NUM> and LiOHH<NUM>O (<NUM>, <NUM> mmol) was added and stirred for <NUM> hour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>,<NUM>-difluoro-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphtho[<NUM>,<NUM>-b]thiophene-<NUM>-carboxylic acid (<NUM>, <NUM> %) as an off-white amorphous.

To a solution of <NUM>-chloro-<NUM>-(<NUM>,<NUM>-diethoxypropoxy)-<NUM>-methylbenzene (<NUM>, <NUM> mmol) in THF (<NUM>)/H<NUM>O (<NUM>) was added Oxone (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> overnight and filtered. The filtrate was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated to give <NUM>-(<NUM>-chloro-<NUM>-methylphenoxy)propanoic acid (<NUM>, <NUM> %) as a white amorphous.

<NUM>-(<NUM>-chloro-<NUM>-methylphenoxy)propanoic acid (<NUM>, <NUM> mmol) was dissolved in trifluoromethansulfonic acid (<NUM>, <NUM> mmol) and the reaction mixture was stirred for <NUM> hours. Ice chip was added slowly and the resulting mixture was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-chloro-<NUM>-methylchroman-<NUM>-one (<NUM>, <NUM> %) as a white amorphous.

The synthesis procedure of Intermediate <NUM>-c was repeated except for using <NUM>-chloro-<NUM>-methylchroman-<NUM>-one (<NUM>, <NUM> mmol) to obtain <NUM>-(bromomethyl)-<NUM>-chlorochroman-<NUM>-one (<NUM>, <NUM> % ) as an ivory solid.

The synthesis procedure of Intermediate <NUM>-d was repeated except for using <NUM>-(bromomethyl)-<NUM>-chlorochroman-<NUM>-one (<NUM>, <NUM> mmol) to obtain <NUM>-chloro-<NUM>-oxochromane-<NUM>-carbaldehyde (<NUM>, <NUM>%) as a white solid.

The synthesis procedure of Intermediate <NUM>-d was repeated except for using <NUM>-chloro-<NUM>-oxochromane-<NUM>-carbaldehyde (<NUM>, <NUM> mmol) to obtain <NUM>-oxo-<NUM>,<NUM>-dihydro-<NUM>-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as an off white solid.

To a solution of methyl <NUM>-oxo-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) in EtOH (<NUM>) was added NaBH<NUM> (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for 2hours. H<NUM>O was added and insoluble white solid was filtered to give methyl <NUM>-hydroxy-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

To a solution of methyl <NUM>-hydroxy-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) in toluene (<NUM>µl) was added SOCl<NUM> (<NUM>µl, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> hours. After cooling, the reaction mixture was concentrated to give methyl <NUM>-chloro-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white amorphous without purification. <NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

A solution of methyl <NUM>-chloro-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol), pyrazole (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) in DMA (<NUM>µl) was stirred at <NUM> for 1hour and then stirred at <NUM>. After 1hour, the reaction mixture was stirred at <NUM> for 1hour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-(<NUM>H-pyrazol-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> %) as a white amorphous.

To a suspension of methyl <NUM>-(<NUM>H-pyrazol-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) in THF (<NUM>µl)/H<NUM>O (<NUM>µl) was added LiOHH<NUM>O (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for 1hour. The reaction mixture was cooled to room temperature. 1N-HCl was added and insoluble white solid was filtered to give <NUM>-(<NUM>H-pyrazol-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM>%) as a white solid. LC/MS ESI (-): <NUM> (M-<NUM>).

To a solution of <NUM>-Pyrrolidinone (<NUM>µl, <NUM> mmol) in DMA (<NUM>) was added NaH (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> minutes. methyl <NUM>-chloro-<NUM>,<NUM>-dihydro-<NUM>-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was added and stirred for 1hour. The reaction mixture was cooled to <NUM>. LiOHH<NUM>O (<NUM>, <NUM> mmol) was added and stirred for 1hour. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-(<NUM>-oxopyrrolidin-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM> %) as a white amorphous.

Methyl <NUM>-hydroxy-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>), and PBr<NUM> (<NUM>, <NUM> mmol) was added. After stirring at room temperature for <NUM> hours, and the reaction mixture was concentrated under a reduced pressure to obtain crude solid compound of methyl <NUM>-bromo-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>. <NUM>H-NMR (<NUM>, DMSO-d<NUM>): δ <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>).

Methyl <NUM>-bromo-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMA (<NUM>), and NaCN (<NUM>, <NUM> mmol) was added at room temperature. The mixture was stirred at <NUM> for <NUM> hours, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-cyano-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as an off-white solid.

Methyl <NUM>-cyano-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in THF/H<NUM>O (<NUM>, <NUM>/<NUM> v/v), and LiOH·H<NUM>O (<NUM>, <NUM> mmol) was added. After stirring at room temperature for <NUM> hours, the reaction mixture was concentrated under a reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain methyl <NUM>-cyano-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-bromo-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in DMA (<NUM>), The mixture was stirred at <NUM> for <NUM> hours, and extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, and concentrated under a reduced pressure. The residue was purified by flash column chromatography (silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain methyl <NUM>-azido-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM>%) as a white solid.

Methyl <NUM>-azido-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylate (<NUM>, <NUM> mmol) was dissolved in THF/H<NUM>O (<NUM>, <NUM>/<NUM> v/v), and LiOH·H<NUM>O (<NUM>, <NUM> mmol) was added. The reaction mixture was stirred at <NUM> for <NUM> hours, 1N HCl (<NUM>) was added, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure to obtain <NUM>-azido-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM>%) as an off-white solid. LC/MS ESI (-): <NUM> (M-<NUM>).

<NUM>,<NUM>-Dichloropyridine-<NUM>-amine (<NUM>, <NUM> mmol) and <NUM>-chlorophenol (<NUM>, <NUM> mmol) were dissolved in sulfolane (<NUM>) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol) was added. The reaction mixture was stirred at <NUM> for <NUM> hours, cooled to room temperature, H<NUM>O was added, and extracted with EtOAc. The organic extract was washed with 1N NaOH aqueous solution and brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain <NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-amine (<NUM>, <NUM>%) as a white solid.

<NUM>-(Methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxylic acid (<NUM>, <NUM> mmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>), and DMF (<NUM>µL, <NUM> mmol) and (COCl)<NUM> (<NUM>µL, <NUM> mmol) were added. The reaction mixture was stirred at <NUM> for <NUM> hours and concentrated under reduced pressure to obtain <NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carbonyl chloride. To the residue, <NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-amine (<NUM>, <NUM> mmol) and <NUM>,<NUM>-Dioxane (<NUM>) were added and the reaction mixture was stirred at <NUM> for <NUM> hours and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxamide (<NUM>, <NUM>%) as a white solid.

Compounds from Examples <NUM> to <NUM> were synthesized through the synthesis route of Example <NUM>, and data of these compounds are listed as follows.

To a solution of <NUM>,<NUM>-dichloropyridin-<NUM>-amine (<NUM>, <NUM> mmol) in sulfolane (<NUM>µl) were added p-cresol (<NUM>, <NUM> mmol) and K<NUM>CO<NUM> (<NUM>, <NUM> mmol). The reaction mixture was stirred at <NUM> for <NUM> hours. The reaction mixture was extracted with EtOAc. The organic extract was washed with 1N-NaOH and brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) and solidification with ACN/ether/Hex to obtain <NUM>-chloro-<NUM>-(p-tolyloxy)pyridin-<NUM>-amine (<NUM>, <NUM> %) as a light brown amorphous. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (brs, <NUM>), <NUM> (s, <NUM>).

The synthesis procedure of Example <NUM>-b was repeated except for using <NUM>-chloro-<NUM>-(p-tolyloxy)pyridin-<NUM>-amine (<NUM>, <NUM> mmol) to obtain N-(<NUM>-chloro-<NUM>-(p-tolyloxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxamide (<NUM>, <NUM> %) as a white amorphous.

The racemate of N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxamide (<NUM>, <NUM> mmol) obtained in Example <NUM> was separated by preparative HPLC (Daicel Chiralpak IA, dichloromethane/ethanol=<NUM>/<NUM>, <NUM>/min, <NUM>, <NUM>) into (S)-N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxamide (<NUM>, <NUM>%) and (R)-N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxamide (<NUM>, <NUM>%).

<NUM>H-NMR (<NUM>, DMSO-d<NUM>): <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM> (s, <NUM>), <NUM>-<NUM> (m, <NUM>), <NUM>-<NUM> (m, <NUM>).

HPLC: Daicel Chiralpak IA, <NUM> I. x <NUM> L, dichloromethane/ethanol=<NUM>/<NUM>, <NUM>/min, <NUM>, <NUM>, tR= <NUM>.

Compounds from Examples <NUM> to <NUM> were synthesized through the synthesis route of Example <NUM> and <NUM>, and data of these compounds are listed as follows.

tert-butyl <NUM>-((<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)carbamoyl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>(<NUM>H)-carboxylate (<NUM>, <NUM>µmol) was dissolved in CH<NUM>Cl<NUM> (<NUM>µl), and TFA (<NUM>µl, <NUM> mmol) was added at <NUM>. The reaction mixture was stirred at room temperature for <NUM> hours. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydrothieno[<NUM>,<NUM>-g]quinoxaline-<NUM>-carboxamide (<NUM>, <NUM> %) as white amorphous.

To a solution ofN-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphtho[<NUM>,<NUM>-b]thiophene-<NUM>-carboxamide (<NUM>, <NUM> mmol) in acetic anhydride (<NUM>, <NUM> mmol) was added chromium oxide(VI) (<NUM>, <NUM> mmol) at <NUM>. The reaction mixture was stirred at <NUM> for <NUM> hours. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(methylsulfonyl)-<NUM>-oxo-<NUM>,<NUM>,<NUM>,<NUM>-tetrahydronaphtho[<NUM>,<NUM>-b]thiophene-<NUM>-carboxamide (<NUM>, <NUM> %) as a white amorphous.

To a suspension of <NUM>-(<NUM>H-pyrazol-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxylic acid (<NUM>, <NUM> mmol) in CH<NUM>Cl<NUM> (<NUM>) were added (COCl)<NUM> (<NUM>µl, <NUM> mmol) and DMF (<NUM>µl, <NUM>µmol). The reaction mixture was stirred at <NUM> for 1hour, and concentrated under reduced pressure to obtain <NUM>-(<NUM>H-pyrazol-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carbonyl chloride. To the residue, <NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-amine (<NUM>, <NUM> mmol) and <NUM>,<NUM>-dioxane (<NUM>) was added. The reaction mixture was stirred at <NUM> overnight. The reaction mixture was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN : <NUM>% formic acid in H<NUM>O) to obtain N-(<NUM>-chloro-<NUM>-(<NUM>-chlorophenoxy)pyridin-<NUM>-yl)-<NUM>-(<NUM>H-pyrazol-<NUM>-yl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>,<NUM>-g]chromene-<NUM>-carboxamide (<NUM>, <NUM> %) as a white solid.

To a suspension of <NUM>-bromo-<NUM>-chloro-<NUM>-nitrobenzene (<NUM>, <NUM> mmol), <NUM>,<NUM>,<NUM>,<NUM>-tetramethyl-<NUM>-(prop-l-en-<NUM>-yl)-<NUM>,<NUM>,<NUM>-dioxaborolane (<NUM>, <NUM> mmol) and Na<NUM>CO<NUM> (<NUM>, <NUM> mmol) in DME (<NUM>)/H<NUM>O (<NUM>) was added Pd(PPh<NUM>)<NUM> (<NUM>, <NUM> mmol). The reaction mixture was refluxed overnight. Pd(PPh<NUM>)<NUM> (<NUM>, <NUM> mmol) was more added and stirred for 4hours. After cooling to room temperature, the reaction mixture was filtered through celite. The filtrate was concentrated and the residue was extracted with EtOAc. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated. The residue was purified by NH-silica column chromatography (hexane only) to give <NUM>-chloro-<NUM>-nitro-<NUM>-(prop-<NUM>-en-<NUM>-yl)benzene (<NUM>) as a crude yellow oil. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (s, <NUM>), <NUM> (s, <NUM>).

To a solution of <NUM>-chloro-<NUM>-nitro-<NUM>-(prop-<NUM>-en-<NUM>-yl)benzene (<NUM>, <NUM> mmol) in Et<NUM>O (<NUM>) was added 33wt% HBr in ACN (<NUM>, <NUM> mmol). The reaction mixture was stirred at room temperature for <NUM> days. NaHCO<NUM> aqueous solution was added under ice bath and the resulting mixture was extracted with Et<NUM>O. The organic extract was washed with sat. NaHCO<NUM> aqueous solution and brine, dried over anhydrous Na<NUM>SO<NUM>, filtered and concentrated. The residue was purified by flash column chromatography (silica gel, n-Hex: EtOAc = <NUM> : <NUM>) to obtain <NUM>-(<NUM>-bromopropan-<NUM>-yl)-<NUM>-chloro-<NUM>-nitrobenzene (<NUM>, <NUM>% in <NUM> steps) as an ivory solid. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (t, <NUM>, J=<NUM>), <NUM> (s, <NUM>).

<NUM>-(<NUM>-Bromopropan-<NUM>-yl)-<NUM>-chloro-<NUM>-nitrobenzene (<NUM>, <NUM> mmol) and chlorobenzene (<NUM>, <NUM> mol) were dissolved in <NUM>,<NUM>-dichloroethane (<NUM>), and AlCl<NUM> (<NUM>, <NUM> mmol) was added. The reaction mixture was stirred at <NUM> for <NUM> hours, H<NUM>O was added, and extracted with CH<NUM>Cl<NUM>. The organic extract was washed with brine, dried over anhydrous Na<NUM>SO<NUM> and concentrated under reduced pressure. The residue was purified by reversed-phase column chromatography (C18-silica gel, <NUM>% formic acid in CH<NUM>CN: <NUM>% formic acid in H<NUM>O) to obtain <NUM>-chloro-<NUM>-(<NUM>-(<NUM>-chlorophenyl)propan-<NUM>-yl)-<NUM>-nitrobenzene (<NUM>, <NUM>%) as a yellow oil. <NUM>H-NMR (<NUM>, CDCl<NUM>): δ <NUM> (t, <NUM>, J=<NUM> ), <NUM> (t, <NUM>, J=<NUM> ), <NUM> (t, <NUM>, J=<NUM> ), <NUM> (d, <NUM>, J=<NUM>), <NUM> (d, <NUM>, J=<NUM>), <NUM> (s, <NUM>).

<NUM>-chloro-<NUM>-(<NUM>-(<NUM>-chlorophenyl)propan-<NUM>-yl)-<NUM>-nitrobenzene (<NUM>, <NUM> mmol) was dissolved in MeOH/THF/H<NUM>O (<NUM>, <NUM>/<NUM>/<NUM> v/v), and Zn (<NUM>, <NUM> mmol) and NH<NUM>Cl (<NUM>, <NUM> mmol) were added at room temperature. The reaction mixture was ultrasonificated at <NUM> for <NUM> minutes, cooled to room temperature, filtered through Celite, and concentrated under reduced pressure. The residue was purified by flash column chromatography (amine silica gel, n-Hex : EtOAc = <NUM> : <NUM>) to obtain <NUM>-chloro-<NUM>-(<NUM>-(<NUM>-chlorophenyl)propan-<NUM>-yl)aniline (<NUM>, <NUM>%) as a yellow oil.

The synthesis procedure of Example <NUM>-b was repeated except for using <NUM>-chloro-<NUM>-(<NUM>-(<NUM>-chlorophenyl)propan-<NUM>-yl)aniline (<NUM>, <NUM> mmol) to obtain N-(<NUM>-chloro-<NUM>-(<NUM>-(<NUM>-chlorophenyl)propan-<NUM>-yl)phenyl)-<NUM>-(methylsulfonyl)-<NUM>,<NUM>-dihydro-<NUM>H-thieno[<NUM>',<NUM>':<NUM>,<NUM>]benzo[<NUM>,<NUM>-b][<NUM>,<NUM>]oxazine-<NUM>-carboxamide (<NUM>, <NUM>%) as a white solid.

Experiments were performed as shown below for the compounds prepared in Examples above.

A human prostate cancer cell line (LNCaP stable cell line; plasmid pSTAT3-TA-luc), which contains a stably operating STAT3 promoter, was cultured in RPMI1640 medium (Cat No. <NUM>, Life Technologies) containing <NUM>% fetal bovine serum (FBS) (Cat No. SH30396, Thermo Scientific) and <NUM>µg/mL G-<NUM> solution (Cat No. <NUM><NUM><NUM><NUM>, Roche). The reporter gene assay using LNCaP stable cell line was performed in RPMI1640 medium containing <NUM>% DCC-FBS without G-<NUM> solution. LNCaP stable cells were plated in two (<NUM>) white <NUM>-well plates with <NUM>,<NUM> cells/<NUM>µL in each well. The cells were cultured at <NUM>, under <NUM>% CO<NUM> for <NUM> hours, and then treated with the compounds listed in Examples which were diluted in various concentrations. Subsequently, IL-<NUM> was added to each well with a final concentration of <NUM> ng/mL. Upon completion of the treatment with the compounds and IL-<NUM>, the cells were cultured at <NUM>, under <NUM>% CO<NUM> for <NUM> hours. The plates were observed under microscope and drug precipitation and particular findings were investigated and recorded.

The luciferase assay and the cell viability assay were performed respectively with one of the two plates. For the luciferase assay, the liquid media in the <NUM>-well plate was removed, and then, <NUM>µL of passive cell lysis buffer was added to each well. After shaking the plate for <NUM> minutes, luciferase activities of each well were measured in a PHERAstar™ microplate reader (BMG LABTECH) using a luciferase assay system (Cat No. E1501, Promega). For the cell viability assay, the <NUM>-well plate was placed at room temperature for <NUM> minutes, added with <NUM>µL/well of CellTiter-Glo solution (Cat No. G7573, Promega), and shaken for <NUM> minutes in order to measure cytotoxicity caused by the compounds listed in Examples with a PHERAstar™ microplate reader (BMG LABTECH). Wells without <NUM>% DMSO and stimulation were used as a negative control and wells with <NUM>% DMSO and stimulation were used as a positive control.

A human osteosarcoma cell line (U2OS stable cell line; pGL4-STAT1-TA-luc), which contains a stably operating STAT <NUM> promoter, was cultured in McCoy <NUM>'A medium (Cat No. <NUM>, Life Technologies) containing <NUM>% FBS (Cat No. SH30396, Thermo Scientific) and <NUM>µg /mL G418 solution (Cat No. <NUM><NUM><NUM><NUM>, Roche). The reporter gene assay using U2OS stable cell line was performed in McCoy <NUM>'A medium containing <NUM>% FBS without G-<NUM> solution. U2OS stable cells were plated in two (<NUM>) white <NUM>-well plates with <NUM>,<NUM> cells/<NUM>µL in each well. The cells were cultured at <NUM>, under <NUM>% CO<NUM> for <NUM> hours, and then treated with the compounds listed in Examples which were diluted in various concentrations. Subsequently, IFN-γ was added to each well with a final concentration of <NUM> ng/mL. Upon completion of the treatment with the compounds and IFN-γ, the cells were cultured at <NUM>, under <NUM>% CO<NUM> for <NUM> hours. The plates were observed under microscope and drug precipitation and particular findings were investigated and recorded.

The luciferase assay and the cell viability assay were performed respectively with one of two plates. For the luciferase assay, the liquid media in the <NUM>-well plate was removed, and then, <NUM>µL of passive cell lysis buffer was added to each well. After shaking the plate for <NUM> minutes, luciferase activities of each well were measured in a PHERAstar™ microplate reader (BMG LABTECH) using a luciferase assay system (Cat No. E1501, Promega). For the cell viability assay, the <NUM>-well plate was placed at room temperature for <NUM> minutes, added with <NUM>µL/well of CellTiter-Glo solution (Cat No. G7573, Promega), and shaken for <NUM> minutes in order to measure cytotoxicity caused by the compounds listed in Examples with a PHERAstar™ microplate reader (BMG LABTECH). Wells without <NUM>% DMSO and stimulation were used as a negative control and wells with <NUM>% DMSO and stimulation were used as a positive control.

The results of evaluation on the inhibitory effect of the compounds listed in the Examples on the dimerization of STAT3 and STAT1 obtained via the STAT3 and STAT1 reporter gene assays are shown in Table <NUM> below.

As shown in Table <NUM>, the compounds according to the present invention exhibited excellent inhibitory effects against the activity of STAT3 protein but showed almost no inhibitory effect against the activity of STAT1 protein.

The inhibitory effects of the compounds of the present invention against the growth of cancer cells were evaluated as shown below. The cancer cell lines including stomach cancer cell line (NCI-N87) and breast cancer cell line (MDA-MB-<NUM>) were cultured under the protocol provided by each supplier. Each type of cells to be used in experiments was subcultured in a <NUM>-well plate by counting the exact number of cells using Tali™ Image-based Cytometer (Life Technologies). In a <NUM>-well plate, NCI-N87 was employed with <NUM>,<NUM> cells/well; and MDA-MB-<NUM> was employed with <NUM>,<NUM> cells/well. The cells were treated with the compounds listed in Examples which were diluted in various concentrations. Upon completion of the compounds treatment, NCI-N87 cells were cultured at <NUM> under <NUM>% CO<NUM> for <NUM> hours, and MDA-MB-<NUM> cells were cultured at <NUM> in air for <NUM> hours. Subsequently, the cells were observed under microscope and drug precipitation and particular findings were investigated and recorded. And then, the <NUM>-well plate was placed at room temperature for <NUM> minutes, added with <NUM>µL/well of CellTiter-Glo solution (Cat No. G7573, Promega) and shaken for <NUM> minutes, followed by being subjected to the measurement using PHERAstar™ microplate reader (BMG LABTECH) according to the supplier's general luminometer protocol. Wells where only culture liquid added without cell plating were used as a negative control, whereas wells where culture liquid containing <NUM>% DMSO instead of the compounds listed in Examples were used as a positive control.

The results of the inhibitory effects of the compounds prepared in Examples against the growth of cancer cells are shown in Tables <NUM> to <NUM> below.

Claim 1:
A compound selected from the group consisting of a heterocyclic derivative represented by formula (I), and a pharmaceutically acceptable salt and a stereoisomer thereof:
<CHM>
wherein
X<NUM> is -C(-Rx)(-Rx")-, -C(-Rx')(-Rx")-, or -N(Rx)-;
X<NUM> is -C(-Rx")(-Rx")-, -C(=O)-, -N(-Rx")-, or -O-;
Rx is
<CHM>
Xs is =O or =NH;
Rx' is haloC<NUM>-<NUM>alkyl, cyano, nitro, amino, azido, or a <NUM>- to <NUM>-membered heterocyclyl containing <NUM> to <NUM> heteroatoms selected from N and O and unsubstituted or substituted with oxo;
Rx" is hydrogen, halogen, C<NUM>-<NUM>alkyl, or C<NUM>-<NUM>alkoxycarbonyl;
Y is -CH= and Z is -S-;
Lx is a saturated C<NUM>-<NUM> hydrocarbon chain not containing or containing O in the chain, and unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, C<NUM>-<NUM>alkyl and C<NUM>-<NUM>alkoxy;
A is benzene or a <NUM>- to <NUM>-membered heteroaryl containing <NUM> to <NUM> nitrogen atoms; B is a monocyclic- or bicyclic-saturated or unsaturated C<NUM>-<NUM>carbocycle or <NUM>- to <NUM>-membered heterocycle;
Rc is =O;
RN is hydrogen;
LB is -[C(-RL)(-RL')]m-, -O-, -NH- or -N(C<NUM>-<NUM>alkyl)-, wherein m is <NUM> or <NUM>, RL and RL' are each independently hydrogen, hydroxy, halogen or C<NUM>-<NUM>alkyl, or RL and RL' are linked together to form C<NUM>-<NUM>alkylene;
RA is halogen, C<NUM>-<NUM>alkoxycarbonylamino-C<NUM>-<NUM>alkoxy, aminoC<NUM>-<NUM>alkoxy, or <NUM>- to <NUM>-membered heterocyclyl;
RB is halogen, C<NUM>-<NUM>alkyl, C<NUM>-<NUM>alkoxy, haloC<NUM>-<NUM>alkyloxy, C<NUM>-<NUM>alkenyloxy, C<NUM>-<NUM>carbocyclyl-oxy, <NUM>- to <NUM>-membered heterocyclyl-C<NUM>-<NUM>alkoxy, or a haloC<NUM>-<NUM>alkyl group;
p is an integer of <NUM> to <NUM>, and, when p is <NUM> or higher, RA moieties are the same as or different from each other;
q is an integer of <NUM> to <NUM>, and, when q is <NUM> or higher, RB moieties are the same as or different from each other; and
each of said heteroaryl, heterocycle and heterocyclyl moieties independently contains <NUM> to <NUM> heteroatoms selected from the group consisting of O, N and S.