Patent Description:
The present disclosure relates to the technical field of medical equipment, and in particular, to a fully automatic light initiated chemiluminescence detector.

Chemiluminescence immunoassay (CLIA) is a technology which combines a chemiluminescence assay technology having high sensitivity with immunoreaction having high specificity and is used for detection analysis of various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs. It is the latest immunoassay technology developed after radioimmunoassay, enzyme immunoassay, fluorescence immunoassay and time-resolved fluorescence immunoassay.

Light initiated chemiluminescence is one of the methods for implementing the chemiluminescence assay. Light initiated chemiluminescence can be used to study the interaction between biomolecules, and it is mainly used for clinical detection of diseases. This technology integrates research in polymer microparticle technology, organic synthesis, protein chemistry, clinical detection and other related fields. Compared with the traditional enzyme-linked immunoassay method, it has such characteristics as being homogeneous-phase, high in sensitivity, simple in operation and easy to automate. Therefore, it has a very broad application prospect.

At present, the light initiated chemiluminescence analyzers in existence have the following problems: (<NUM>) low detection flux, so they cannot meet requirements of clinical detection in large-scale hospitals; (<NUM>) relatively long detection time, so a report cannot be provided in a short time; (<NUM>) low degree of automation, so there are relatively large individual differences due to manual operation; and (<NUM>) the false-reportin detection results due to the "HOOK" effect cannot be avoided. The HD-HOOK effect often occurs in immunoassays, and its incidence accounts for about <NUM>% of positive samples. Due to the existence of the HD-HOOK effect, it cannot be accurately distinguished whether the result is obtained because the concentration of the detected sample exceeds the linear range of the detection kit or because the concentration of the detected sample is indeed the value, which leads to an increase in the experimental misdiagnosis, especially the false negative rate. <CIT> discloses an automatic analyzer includes a reaction unit configured for holding a reaction container and carrying the reaction container to a determined operation position, the operation position including a detection operation position; a detection unit configured for detecting analyte in the reaction container of the reaction unit in the detection operation position; a bound-free ("B/F") unit configured for removing unbound components of a reaction system; and a dispensing unit configured for dispensing reagent and/or a sample to the reaction container, wherein the reaction unit includes an incubation position for incubating a solution in the reaction container. <CIT> discloses a container supply device having: a tilted transportation path for transporting containers, which are arranged in a line, from the upstream to a container stand-by position located downstream; a partitioning member provided with a first partitioning plate which enters into and exits from the transportation track of the tilted transportation path, and a second partitioning plate which is located upstream the first partitioning plate and which is at an exit position relative to the transportation track when the first partitioning plate enters the transportation track and is at an enter position relative to the transportation track when the first partitioning plate exits the transportation track; and a driving means which pushes and pulls the partitioning member and which brings the partitioning member into or out of the transportation track. <CIT> provides a container supply unit comprising a container storage part, a container discharge part and a container alignment part. The container alignment part comprises an alignment rail. The alignment rail has a conveyance speed securing region and a posture stabilizing region. The conveyance speed securing region is inclined to a horizontal direction. The posture stabilizing region is provided successively downstream from the conveyance speed securing region in a conveyance direction, and set to a smaller angle of inclination to the horizontal direction than the conveyance speed securing region. Then a conveyance position is provided downstream from the posture stabilizing region in the conveyance direction and arranged in parallel with the horizontal direction.

The goal underlying the present invention is to provide an improved or ameliorated system of a chemiluminescence detector. The goal can be reached by the subject matter of the present invention and as further exemplified by the description and the claims.

The subject matter in accordance with the claims and/or the description attains this goal.

The present disclosure provides a fully automatic light initiated chemiluminescence detector which can effectively avoid problems such as individual differences, manual errors, non-standard actions and other uncertainties in the manual operation and improve the accuracy of chemical analysis. We were surprised to find that the fully automatic light initiated chemiluminescence detector of the present disclosure can effectively solve the problem of false-reporting detection results due to the "HOOK" effect.

The fully automatic light initiated chemiluminescence detector comprises a sample loading module, a sampling arm module, an incubation disk module, a reagent disk module, a reagent arm module and a detection module. A sample in the sample loading module is transferred to a reaction cuvette by means of the sampling arm module. After the reaction cuvette arrives at a specified position, the reagent arm module transfers a reagent in the reagent disk module to the reaction cuvette. The sample and the reagent in the reaction cuvette are mixed to be incubated at the incubation disk by a mixing mechanism. After the incubation, the detection module sends light on a substance to be detected in the reaction cuvette to initiate a reaction between the sample and the reagent, and detects a luminescence signal generated after the initiation. The fully automatic light initiated chemiluminescence detector further comprises a cuvette sorting module and a cuvette delivering module located between the cuvette sorting module and the incubation disk module. The cuvette sorting module is configured to sort disordered reaction cuvettes into ordered reaction cuvettes, which are delivered to the incubation disk module in sequence via the cuvette delivering module. The cuvette delivering module comprises a slide rail, a cuvette holding device slidably connected to the slide rail, a transmission device for actuating a movement of the cuvette holding device along the slide rail, a slide channel located below the cuvette holding device, and a reset device enabling the reset of the cuvette holding device. The cuvette holding device is configured to control a movement of the reaction cuvette, so that the cuvette is delivered horizontally directly from the cuvette sorting module through the slide channel to the incubation disk module.

In an embodiment, the cuvette sorting module locates behind the sampling arm module. The cuvette sorting module has a cuvette accommodation part for placing the reaction cuvette and a cuvette off-loading device set at the bottom of the cuvette accommodation part. The cuvette off-loading device comprises a reaction cuvette rotating disk. A cuvette groove, which matches the reaction cuvette, is provided at the circumferential edge at the top of the reaction cuvette rotating disk.

In an embodiment, the cuvette off-loading device includes a first actuating part to actuate the off-load of the reaction cuvette from the cuvette groove. The first actuating part is set at the side of the reaction cuvette rotating disk and includes a toggle wheel to actuate the offload of the reaction cuvette from the cuvette groove. A first cuvette off-loading groove, which matches the reaction cuvette, is provided on the toggle wheel.

Preferably, the first actuating part includes a cuvette off-loading member to actuate the off-load of the reaction cuvette from the cuvette groove. The cuvette off-loading member is connected to the toggle wheel via a first connecting shaft, and a second cuvette off-loading groove, which matches the reaction cuvette, is provided on the cuvette off-loading member. The second cuvette off-loading groove is parallel to the first cuvette off-loading groove.

In an embodiment, the first actuating part includes a linkage mechanism to actuate the periodic rotation of the toggle wheel, and the bottom a toggle groove corresponding to the cuvette groove is provided at a circumferential edge at the bottom of the reaction cuvette rotating disk. The linkage mechanism includes:.

In an embodiment, the first connecting shaft is sheathed with a torsion spring to realize automatic resetting of the toggle wheel. A first end of the torsion spring is fastened to the reaction cuvette slide channel base, and a second end of the torsion spring is fastened to the toggle wheel.

In an embodiment, a reaction cuvette slide channel, which leads to the cuvette groove, is provided inside of the reaction cuvette slide channel base. A longitudinal guide groove is provided on the reaction cuvette slide channel, and a horizontal guide groove is provided at an end of the longitudinal guide groove on the reaction cuvette slide channel.

In an embodiment, the reaction cuvette rotating disk is rotatable in a tray. The bottom of the tray is fastened to the reaction cuvette slide channel base, and a first actuating device, which actuates the rotation of the rotating disk to rotate, is provided at the bottom of the tray.

In an embodiment, a second actuating device is provided near to the horizontal guide groove on the reaction cuvette slide channel base, and the second actuating device controls the movement of the reaction cuvette from a start of the horizontal guide groove to an end of the horizontal guide groove.

In an embodiment, the cuvette delivering module locates between the cuvette sorting module and the incubation disk module. The cuvette delivering module includes a slide rail, a cuvette holding device, a transmission device, a slide channel and a reset device. The cuvette holding device is slidably connected to the slide rail; the transmission device can actuate the movement of the cuvette holding device along the slide rail; the slide channel locates below the cuvette holding device, and the reaction cuvette locates inside the slide channel; the cuvette holding device can control the movement of the reaction cuvette; and the reset device can enable the reset of the cuvette holding device.

Preferably, the cuvette holding device includes a slider, a reaction cuvette claw, an electromagnet and a first photoelectric sensor. The slide rail is slidably engaged with the slider; the claw is set at one side of the slider and slides vertically relative to the slider; the electromagnet is set above the claw and is fixedly connected to the slider; and the first photoelectric sensor is set at one side of the claw and is fixedly connected to the slider.

In an embodiment, the sample loading module includes a baseplate, several test tube racks and a moving device. The test tube racks and the moving device are set on the baseplate, and the test tube racks are moved to specified positions by means of the moving device.

In an embodiment, the moving device includes an X-axis pushing mechanism. The X-axis pushing mechanism includes a circulation pushing member, which actuates the operation of a synchronous belt by means of a motor.

In an embodiment, blocking pieces are provided with intervals on the synchronous belt along the direction of the synchronous belt and the circulation pushing member pushes the test tube racks forward section by section by means of the synchronous belt having the blocking pieces.

In an embodiment, the moving device further includes a Y-axis pushing mechanism. The Y-axis pushing mechanism is a conveyor device, which is driven by a motor and conveys the test tube rack to the circulation pushing member.

In an embodiment, the X-axis pushing mechanism further includes a recycling pushing member to recycle a sampled test tube.

In an embodiment, the recycling pushing member locates opposite to the circulation pushing member, and the moving direction of the test tube rack pushed by the recycling pushing member is counter to the moving direction of the test tube rack pushed by circulation pushing member.

In an embodiment, the conveyor device includes a first conveyor belt and a second conveyor belt, conveying directions of which are counter to each other. The second conveyor belt conveys the test tube rack to the circulation pushing member, and the sampled test tube rack is conveyed to the recycling pushing member by the first conveyor belt.

Preferably, a sample loading module further includes a test tube barcode scanner, a test tube type discriminator, and a positioning device to determine a position for sampling a test tube. A scanning direction of the test tube barcode scanner and a discrimination direction of the test tube type discriminator face the test tube rack.

In an embodiment, the first conveyor belt and the second conveyor belt, conveying directions of which are counter to each other, are respectively set at two ends of the sample loading module. The test tube rack is set on the conveyor belt. A moving device includes a circulation pushing member and a recycling pushing member. The circulation pushing member and the recycling pushing member are set between the first conveyor belt and the second conveyor belt, and are respectively located at opposite sides of the rail sample loading module. The test tube rack is conveyed from the second conveyor belt to the circulation pushing member, and the circulation pushing member pushes the test tube rack to a test tube scanning component; after scanning and sampling, the test tube rack is transported to the first conveyor belt; and then the test tube rack is pushed back to the second conveyor belt by recycling pushing member.

In an embodiment, the sampling arm module includes a frame and an arm assembly. The arm assembly is set at an upper portion of the frame, and a vertical motion assembly and a rotary motion assembly, which are connected to the arm assembly, are provided on the frame. The vertical motion assembly enables the vertical movement of the arm assembly relative to the frame, and the rotary motion assembly enables the rotation of the arm assembly relative to the frame.

In an embodiment, the arm assembly includes a connecting arm. One end of the connecting arm is fixedly connected to a sample needle, and the other end of the connecting arm is fixedly connected to a spline shaft. The sample needle and the spline shaft locate on the same side of the connecting arm.

In an embodiment, the incubation disk module includes a first incubation disk and a second incubation disk, and both incubation disks rotate in motor-driven manner. A cuvette removing and discarding module is provided between the first incubation disk and the second incubation disk, the cuvette removing and discarding module includes a cuvette pushing rail and a cuvette discarding rail, and the cuvette pushing rail and the cuvette discarding rail are switched by means of a linear motor.

In an embodiment, the reagent disk module includes an open device and a first rotating part, which is set at the bottom of the open device and drives the open device to rotate. The side wall of the open device is provided with a scanning part, and a scanning device is capable of identifying an attribute of a reagent inside the open device by means of the scanning part.

In an embodiment, the bottom a rotation connecting partis provided at the bottom of the open device. The rotation connecting part is connected to the first rotating part, and a fixation seat is fixedly set at the rotation connecting part.

In an embodiment, a positioning device to obtain the position of a reagent compartment is provided on the first rotating part.

In an embodiment, the positioning device includes a sensor, which is fixed on a support body, and a sensor light-blocking sheet, which is set at the bottom of a rotation shaft.

In an embodiment, the reagent in a reagent disk is transferred to the reaction cuvette by means of a fluid system under the control of the reagent arm module. The fluid system includes:.

Preferably, the dispensing and rinsing system includes:.

In an embodiment, the acid cleaning system includes a sample needle cleaning tank and reagent needle cleaning tanks. An inlet of the sample needle cleaning tank and an inlet of the reagent needle cleaning tanks are respectively connected to each of outlets of acid cleaning liquid pumps. Inlets of the acid cleaning liquid pumps are respectively connected to first check valves, and the first check valves are all connected to an acid cleaning liquid bottle. An outlet of the sample needle cleaning tank and an outlet of the reagent needle cleaning tanks are respectively connected to the waste discharging system.

In an embodiment, the waste discharging system includes a negative pressure tank. A liquid inlet of the negative pressure tank is respectively connected to an outlet of a sample needle cleaning tank, and outlets of reagent needle cleaning tanks. A liquid outlet of the negative pressure tank is connected to an inlet of a waste liquid discharging pump via a third solenoid valve. An outlet of the waste liquid discharging pump is connected to an inlet of a waste liquid bottle. An air outlet of the negative pressure tank is connected to an inlet of the negative pressure pump.

In an embodiment, the detection module includes a light path detection system and a control system. The light path detection system includes:.

The second actuating part actuates the opening and closing of the excitation light pathway and the signal light pathway at the same time. When the excitation light pathway is opened, the signal light pathway is closed, and when the excitation light pathway is closed, the signal light pathway is opened.

In an embodiment, the excitation light pathway switch includes a second rotating part. Through holes are provided at a circumferential side wall of a first end of the second rotating part, which through holes are used to realize conduction of the excitation light, and a second end of the second rotating part is fastened to output shaft of a first end of the second actuating part.

Preferably, there are two through holes, and the two through holes at the circumferential side wall of the first end of the second rotating part are opposite to each other.

In an embodiment, the light path detection system includes an excitation unit. The excitation unit includes a first housing base. The first end of the second rotating part extends through one side wall of the first housing base into the interior of the first housing base; the through holes are set inside the first housing base; the circumferential side wall of the second rotating part is rotatably connected to one side wall of the first housing base; and the top and the bottom of the first housing base are both provided with an excitation light channel.

In an embodiment, a transflective lens, which transmits the excitation light and reflects luminescence signals generated by the substance to be detected after the excitation with the excitation light, is set at a position at the side of the second rotating part and away from the excitation unit.

In an embodiment, a first lens, which focuses the luminescence signals generated by the substance to be detected, is set at the side of the transflective lens, and the first lens is close to the signal light pathway switch.

Preferably, tan optical filter is provided at the side of the first lens close to the signal light pathway switch.

In an embodiment, the transflective lens, the first lens and the optical filter are all set inside a second housing base. The top of the second housing base is fastened to the bottom of the first housing base; a first opening is provided on one side wall of the second housing base, which first opening transmits the luminescence signals generated by the substance to be detected and leads to a signal light pathway switch; and a second opening is provided at the bottom of the second housing base, which second opening is set to match the substance to be detected.

In an embodiment, the excitation unit includes a laser to emit the excitation light, and the excitation light emitted by the laser excites, multiple times, the substance to be detected, such that the substance to be detected generates a plurality of luminescence signals. The light path detection system further includes the receiving and detecting unit. The receiving and detecting unit includes a detector, which detects, multiple times, the luminescence signals generated by the substance to be detected and records corresponding detection results.

In an embodiment, a second lens to focus the excitation light is set at a position between the laser and the second rotating part.

In an embodiment, the signal light pathway switch includes:.

In an embodiment, the crank linkage device includes:.

The first rotating component and the second rotating component are both rotatably connected to the baffle.

In an embodiment, when the excitation light is conducted to excite the substance to be detected, the fourth opening is staggered with the first opening and the third opening, and at this time, the signal light pathway switch is in a closed state.

In an embodiment, when the luminescence signals generated by the substance to be detected enter the receiving and detecting unit, the first opening and the third opening are aligned with the fourth opening, and a signal light pathway switch is in an opened state; and at this time, the excitation light pathway switch blocks the excitation light.

In an embodiment, the fully automatic light initiated chemiluminescence detector further includes an emergency position. A corresponding sensor is set on the emergency position. When the sensor senses that an emergency sample is placed at the emergency position, the sampling arm module sucks the sample at the emergency position first to perform detection of the emergency sample. After sucking of the emergency sample is finished, sucking of the sample in the ordinary rail sample loading module is continued.

In an embodiment, the fully automatic light initiated chemiluminescence detector further includes a sample diluent compartment. There are at least two kinds of sample diluents in the sample diluent compartment, and the sampling arm module sucks the diluent to dilute the sample.

Compared with the existing technologies, the fully automatic light initiated chemiluminescence detector of the present disclosure has the following advantages.

The above technical features can be combined in various suitable ways or replaced by equivalent technical features, as long as the objective of the present disclosure can be achieved.

The present disclosure will be described in a more detailed way below based on embodiments, which are only non-limiting embodiments, and with reference to the accompanying drawings, in which:.

In the accompanying drawings, same components are indicated by same reference signs. The accompanying drawings are not drawn according to actual proportions.

In the drawings, indications of respective reference signs are as follows:
<NUM>. rail sample loading module; <NUM>. emergency position; <NUM>. sampling arm module; <NUM>. sample diluent compartment; <NUM>. first incubation disk; <NUM>. first reagent arm; <NUM>. fluid system; <NUM>. first reagent disk; <NUM>. second reagent disk; <NUM>. control system; <NUM>. second reagent arm; <NUM>. light path detection system; <NUM>. second incubation disk; <NUM>. cuvette removing and discarding module; <NUM>. third reagent arm; <NUM>. cuvette delivering module; <NUM>. cuvette sorting module; <NUM>. frame; <NUM>. second check valve; <NUM>. sample needle external rinsing water pump; <NUM>. first solenoid valve; <NUM>. sample needle dispensing pump; <NUM>. sample needle internal rinsing water pump; <NUM>. reagent needle dispensing pump; <NUM>. reagent needle; <NUM>. second solenoid valve; <NUM>. reagent needle internal rinsing water pump; <NUM>. air filter; <NUM>. clean water tank; <NUM>. first liquid level sensor; <NUM>. water inlet pump; <NUM>. water inlet valve; <NUM>. reagent needle cleaning tank; <NUM>. sample needle cleaning tank; <NUM>. acid cleaning liquid pump; <NUM>. first check valve; <NUM>. acid cleaning liquid bottle; <NUM>. second liquid level sensor; <NUM>. silencer; <NUM>. pressure sensor; <NUM>. negative pressure pump; <NUM>. negative pressure tank; <NUM>. third liquid level sensor; <NUM>. third solenoid valve; <NUM>. waste liquid discharging pump; <NUM>. recycling pushing member; <NUM>. first conveyor belt; <NUM>. test tube rack; <NUM>. first positioning device; <NUM>. test tube type discriminator; <NUM>. circulation pushing member; <NUM>. test tube barcode scanner; <NUM>. second conveyor belt; <NUM>. excitation unit; <NUM>. excitation light pathway switch; <NUM>. second actuating part; <NUM>. signal light pathway switch; <NUM>. detection member; <NUM>. optical filter; <NUM>. first lens; <NUM>. transflective lens; <NUM>. laser; <NUM>. second lens; <NUM>. lens holder; <NUM>. laser holder; <NUM>. excitation light channel; <NUM>. first housing base; <NUM>. second rotating part; <NUM>. second housing base; <NUM>. second opening; <NUM>. crank linkage device; <NUM>. baffle; <NUM>. third opening; <NUM>. fourth opening; <NUM>. first rotating component; <NUM>. second rotating component; <NUM>. through hole; <NUM>. reaction cuvette; <NUM>. reaction cuvette claw; <NUM>. stepper motor; <NUM>. synchronous belt wheel; <NUM>. first synchronous belt; <NUM>. idler wheel; <NUM>. slide rail; <NUM>. electromagnet; <NUM>. first photoelectric sensor; <NUM>. slide channel; <NUM>. reset device; <NUM>. slider; <NUM>. sliding groove; <NUM>. cuvette groove; <NUM>. cuvette accommodation part; <NUM>. reaction cuvette rotating disk; <NUM>. reaction cuvette slide channel; <NUM>. first actuating part; <NUM>. first actuating device; <NUM>. longitudinal guide groove; <NUM>. horizontal guide groove; <NUM>. friction wheel; <NUM>. electromagnet guide part; <NUM>. limit bar; <NUM>. first cuvette off-loading groove; <NUM>. reaction cuvette slide channel base; <NUM>. second cuvette off-loading groove; <NUM>. sample needle; <NUM>. connecting arm; <NUM>. spline shaft; <NUM>. rotary motion assembly; <NUM>. vertical motion assembly; <NUM>. first motor; <NUM>. first driving wheel; <NUM>. second synchronous belt; <NUM>. moving block; <NUM>. second motor; <NUM>. second driven wheel; <NUM>. rotating block; <NUM>. toggle wheel; <NUM>. cuvette off-loading member; <NUM>. first connecting shaft; <NUM>. linkage mechanism; <NUM>. torsion spring; <NUM>. first toggle bar; <NUM>. first rotating shaft; <NUM>. first linking bar; <NUM>. second linking bar; <NUM>. second connecting shaft; <NUM>. fixation seat; <NUM>. test container; <NUM>. open device; <NUM>. refrigeration device; <NUM>. scanning part; <NUM>. first rotating part; <NUM>. support body; <NUM>. sensor; <NUM>. rotation shaft; <NUM>. sensor light-blocking sheet; and <NUM>. second positioning device.

The present disclosure will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted that, as long as there is no conflict, respective embodiments in the present disclosure and respective features in the respective embodiments can be combined with one another, and the obtained technical solutions are within the protection scope of the present disclosure.

As shown in <FIG>, a fully automatic light initiated chemiluminescence detector includes a sample loading module <NUM>, a sampling arm module <NUM>, an incubation disk module, a reagent disk module, and a reagent arm module. The sample loading module <NUM> locates at one side of a frame <NUM>, and the sampling arm module <NUM> locates at one side of the sample loading module <NUM>. The sampling arm module <NUM> transfers a sample in the sample loading module <NUM> to a reaction cuvette <NUM> located on the incubation disk module. After the reaction cuvette 160is rotated along with an incubation disk and arrives at a specified position, the reagent arm module located between the reagent disk module and the incubation disk module transfers a reagent in the reagent disk module to the reaction cuvette. The sample and the reagent in the reaction cuvette <NUM> are mixed in the incubation disk, and transferred to a detection module after the incubation.

In an embodiment, the fully automatic light initiated chemiluminescence detector of the present disclosure further includes a cuvette sorting module <NUM>. As shown in <FIG>, the cuvette sorting module <NUM> locates behind the sampling arm module <NUM>, and the cuvette sorting module <NUM> includes a cuvette accommodation part <NUM> for accommodating the reaction cuvette and a cuvette off-loading device located at the bottom of the cuvette accommodation part <NUM>. An opening is set on the side of the cuvette accommodation part <NUM>, and the opening extends along the side of the cuvette accommodation part <NUM> from the top to the bottom. The opening transits in an arc shape along the side of the cuvette accommodation part <NUM> from the top to the middle, so that a volume of the cuvette accommodation part <NUM> gradually increases from the top to the middle. An inner wall of the cuvette accommodation part <NUM> is set to be smooth. Under action of the smooth inner wall of the cuvette accommodation part <NUM>, the reaction cuvette <NUM> is enabled to enter the cuvette off-loading device quickly.

The cuvette off-loading device includes a tray fastened to the bottom of the cuvette accommodation part <NUM> and a reaction cuvette rotating disk <NUM> set in the tray. A cuvette groove, is provided at a circumferential edge of the top of the reaction cuvette rotating disk <NUM> (a position where the reaction cuvette rotating disk <NUM> is in contact with the reaction cuvette <NUM>), which matches the reaction cuvette <NUM>. A first actuating part <NUM> to actuate the off-load of the reaction cuvette <NUM> from the cuvette groove <NUM> is set at one side of the reaction cuvette rotating disk <NUM>. The reaction cuvette rotating disk <NUM> is driven to rotate by a first actuating device <NUM>. Under action of rotation the reaction cuvette rotating disk <NUM>, the reaction cuvette <NUM> located at the bottom of the cuvette accommodation part <NUM> can be placed in the cuvette groove <NUM> in sequence, and then under action of the first actuating part <NUM>, the reaction cuvette <NUM> is offloaded from the cuvette groove <NUM> uniformly.

As shown in <FIG>, the first actuating part <NUM> includes a toggle wheel <NUM> to actuate the reaction cuvette <NUM> to be offloaded from the cuvette groove <NUM>, and the toggle wheel <NUM> is provided thereon with a first cuvette off-loading groove <NUM> which matches the reaction cuvette <NUM>. During rotation of the reaction cuvette rotating disk <NUM>, the reaction cuvette <NUM> can be offloaded from the cuvette groove <NUM> in sequence by means of the first cuvette off-loading groove <NUM>.

As shown in <FIG>, the first actuating part <NUM> further includes a cuvette off-loading member <NUM> to actuate the off-load of the reaction cuvette <NUM> from the cuvette groove <NUM>. The cuvette off-loading member <NUM> is connected to the toggle wheel <NUM> via a first connecting shaft <NUM> (a central shaft for rotation of the toggle wheel <NUM>). A second cuvette off-loading groove <NUM>, which matches the reaction cuvette <NUM>, is provided on the cuvette off-loading member <NUM>. The second cuvette off-loading groove <NUM> is parallel to the first cuvette off-loading groove <NUM>. Under combined action of the second cuvette off-loading groove <NUM> and the first cuvette off-loading groove <NUM>, the reaction cuvette <NUM> can be offloaded from the cuvette groove <NUM> smoothly. The first cuvette off-loading groove <NUM> matches an annular flange on the top of the reaction cuvette <NUM>, and the second cuvette off-loading groove <NUM> matches the bottom of a cuvette body of the reaction cuvette <NUM>. In the process of the offload of the reaction cuvette <NUM>, tilting of the reaction cuvette <NUM> can be prevented, so that the reaction cuvette <NUM> can be offloaded smoothly in a lying position.

As shown in <FIG> and <FIG>, the first actuating part <NUM> further includes a linkage mechanism <NUM> to actuate the periodic rotation of the toggle wheel. During anticlockwise rotation of the reaction cuvette rotating disk <NUM>, periodical anticlockwise rotation of the toggle wheel can be realized.

A toggle groove <NUM> corresponding to the cuvette groove <NUM> (as shown in <FIG> and <FIG>) is provided at a circumferential edge at the bottom of the reaction cuvette rotating disk <NUM>. As shown in <FIG>, the linkage mechanism <NUM> includes a first toggle bar <NUM>. A first end of the first toggle bar <NUM> is slidable in the toggle groove <NUM>, and a second end of the first toggle bar <NUM> is fastened to a first rotating shaft <NUM>. The first rotating shaft <NUM> is rotatably connected to a reaction cuvette slide channel base <NUM>, and the reaction cuvette slide channel base <NUM> is fixedly set at the side of the reaction cuvette rotating disk <NUM>. A first linking bar <NUM> is fastened to the first rotating shaft <NUM>, and a free end of the first linking bar <NUM> is fastened to a first end of a second linking bar <NUM>, a second end of the second linking bar <NUM> being connected to the toggle wheel <NUM> via a second connecting shaft <NUM>. When the reaction cuvette rotating disk <NUM> rotates anticlockwise, the first toggle bar <NUM> is driven to rotate clockwise periodically under action of the toggle groove <NUM>. A specific process is as follows. During anticlockwise rotation of the reaction cuvette rotating disk <NUM>, when the first end of the first toggle bar <NUM> locates in the toggle groove <NUM> and is not in contact with a groove wall of the toggle groove <NUM> (as shown in <FIG>), the first toggle bar <NUM> does not rotate; when the first end of the first toggle bar <NUM> is in contact with the groove wall of the toggle groove <NUM> (as shown in <FIG>), the first toggle bar <NUM> starts to rotate clockwise; and when the first end of the first toggle bar <NUM> is in contact with the groove wall of the toggle groove <NUM> again, the first toggle bar <NUM> starts to rotate clockwise again, so that periodical clockwise rotation of the first toggle bar <NUM> is realized. When the first toggle bar <NUM> rotates clockwise, the first rotating shaft <NUM> and the first linking bar <NUM> are driven to rotate; the first linking bar <NUM> drives the second linking bar <NUM> to move horizontally; and the second linking bar <NUM> actuates the toggle wheel <NUM> to rotate anticlockwise via the second connecting shaft <NUM>. The first toggle bar <NUM> rotates periodically so as to actuate the periodic rotation of the toggle wheel <NUM>.

The first connecting shaft <NUM> is sheathed with a torsion spring <NUM> realize automatic resetting of the toggle wheel <NUM>. A first end of the torsion spring <NUM> is fastened to the reaction cuvette slide channel base <NUM>, and a second end of the torsion spring <NUM> is fastened to the toggle wheel <NUM>. The toggle wheel <NUM> rotates anticlockwise so as to actuate the first cuvette off-loading groove <NUM> and the second cuvette off-loading groove <NUM> to jointly offload the reaction cuvette <NUM>. Under action of the torsion spring <NUM>, the toggle wheel <NUM> is enabled to rotate back clockwise so as to reset.

A limit bar <NUM> to ensure the normal reset of the toggle wheel <NUM> is further set on the toggle wheel <NUM>. When the toggle wheel <NUM> rotates back clockwise to a normal state, a free end of the limit bar <NUM> is blocked by a side wall of the reaction cuvette slide channel base <NUM>, so as to prevent excessive rotation of the toggle wheel <NUM> when the toggle wheel <NUM> rotates back clockwise to reset.

As shown in <FIG> and <FIG>, a reaction cuvette slide channel <NUM>, which leads to the cuvette groove <NUM>, is provided inside of the reaction cuvette slide channel base <NUM>. A longitudinal guide groove <NUM> is provided on the reaction cuvette slide channel <NUM>. A horizontal guide groove <NUM>, which is in communication with the longitudinal guide groove <NUM>, is transversely set at the bottom of the longitudinal guide groove <NUM>. A second actuating device to actuate the reaction cuvette <NUM> in the horizontal guide groove <NUM> sliding along the horizontal guide groove <NUM> to an end thereof in turn, is set at one side of the horizontal guide groove <NUM>.

In an embodiment, the second actuating device includes a driving motor and a friction wheel <NUM> which is fastened to an output shaft of the driving motor and is used to control the movement of the reaction cuvette <NUM>. The driving motor drives the friction wheel <NUM> to rotate, and during rotation of the friction wheel <NUM>, a circumferential side surface of the friction wheel <NUM> is in contact with a flange of the reaction cuvette <NUM>. By means of an acting force between the friction wheel <NUM> and the reaction cuvette <NUM>, the reaction cuvette <NUM> is driven to move along a horizontal guide groove <NUM> to the end thereof in an upright position.

Preferably, an electromagnet guide part <NUM> to control the reaction cuvette to be offloaded from the horizontal guide groove <NUM> uniformly is set at the end of the horizontal guide groove <NUM>, and a contact sensor is set at a start of the electromagnet guide part <NUM>.

The reaction cuvette in the horizontal guide groove <NUM> stops moving when being in contact with the contact sensor of the electromagnet guide part <NUM>.

The cuvette sorting module <NUM> is capable of sorting disordered reaction cuvettes into ordered reaction cuvettes, and the ordered reaction cuvettes are delivered to the first incubation disk <NUM> in sequence via a cuvette delivering module <NUM>, which improves the detection efficiency of the samples. The reaction cuvette <NUM> is first put into the cuvette accommodation part <NUM> through the opening, and the first actuating device actuates the reaction cuvette rotating disk <NUM> to rotate anticlockwise, so that the reaction cuvette <NUM> in the cuvette accommodation part <NUM> enters the cuvette groove <NUM> in sequence. The reaction cuvette rotating disk <NUM> rotates anticlockwise to drive the toggle wheel <NUM> to rotate anticlockwise periodically, so that the reaction cuvette <NUM> in the cuvette groove <NUM> can be transported to the reaction cuvette slide channel <NUM> in sequence, in a lying position, and uniformly; and then the reaction cuvette <NUM> enters the longitudinal guide groove <NUM> and the horizontal guide groove <NUM> sequentially. When a malfunction caused by a stuck cuvette occurs at the reaction cuvette rotating disk <NUM>, idling of a first motor is realized under action of a friction clutch in the first actuating device, so that the reaction cuvette <NUM> stuck at the reaction cuvette rotating disk 172can be taken out conveniently. When the quantity of the reaction cuvette <NUM> in the longitudinal guide groove <NUM> reaches a level where a sensor indicates that the longitudinal guide groove <NUM> is filled therein with the reaction cuvette <NUM>, the reaction cuvette rotating disk <NUM> stops rotating. A second motor drives the friction wheel <NUM> to move, and ensures that the friction wheel <NUM> is in contact with the reaction cuvette <NUM>, so as to control the reaction cuvette <NUM> to move from a start of the horizontal guide groove <NUM> to the end thereof in sequence, in an upright position, and uniformly. After the reaction cuvette <NUM> at the end of the horizontal guide groove <NUM> is in contact with a contact sensor, the reaction cuvette <NUM> stops moving, so as to achieve the objective of sorting disordered reaction cuvettes <NUM>.

In an embodiment, the fully automatic light initiated chemiluminescence detector of the present disclosure further includes the cuvette delivering module. As shown in <FIG> and <FIG>, the cuvette delivering module locates between the cuvette sorting module <NUM> and the incubation disk module, and includes a slide rail <NUM>, a cuvette holding device, a transmission device, a slide channel and a reset device <NUM>. The cuvette holding device is slidably connected to the slide rail <NUM>. The transmission device locates above the slide rail <NUM>, and can control the movement of the cuvette holding device along the slide rail <NUM>. The slide channel <NUM> locates below the cuvette holding device, and the reaction cuvette <NUM> locates inside the slide <NUM>. The cuvette holding device can control the movement of the reaction cuvette <NUM> inside the slide <NUM>. The reset device <NUM> can enable the reset of the cuvette holding device. Preferably, the reset device <NUM> is fixed above one end of the slide rail <NUM>.

The cuvette holding device includes: a slider <NUM>, a reaction cuvette claw <NUM>, an electromagnet <NUM> and a first photoelectric sensor <NUM>. The slider <NUM> is slidably engaged with the slide rail <NUM>. The reaction cuvette claw <NUM> is set at one side of the slider <NUM> and the reaction cuvette claw <NUM> slides vertically relative to the slider <NUM>. The reaction cuvette claw <NUM> is provided with a sliding groove <NUM> which allows a stop screw to pass through, and the stop screw passes through the sliding groove <NUM> and connects the reaction cuvette claw <NUM> to the slider <NUM>. An electromagnet <NUM>, which controls the reaction cuvette claw <NUM> to slide vertically relative to the slider <NUM>, is set above the reaction cuvette claw <NUM>. The electromagnet <NUM> is fixedly connected to the slider <NUM>. When the electromagnet <NUM> is powered on, the reaction cuvette claw <NUM> goes away from electromagnet <NUM> and moves downward along the sliding groove <NUM>. When the electromagnet <NUM> is not powered on, the reaction cuvette claw <NUM> is adsorbed at a lower end of the electromagnet <NUM>. The first photoelectric sensor <NUM> is set at one side of the reaction cuvette claw <NUM>, and is used to detect a position of the reaction cuvette claw <NUM>.

As shown in <FIG>, the bottom of the reaction cuvette <NUM> is in a hemispherical shape, and the cuvette body of the reaction cuvette <NUM> is in a cylindrical shape. A cylindrical projection is provided in the center of the bottom of the reaction cuvette <NUM>. A cuvette rim projecting outward is provided around the circumference of the opening of the reaction cuvette <NUM>, and a diameter of the cuvette rim is greater than a diameter of the cuvette body of the reaction cuvette <NUM>. A limit groove is set at the bottom of the reaction cuvette claw <NUM>, and the limit groove engages the reaction cuvette <NUM>. The limit groove has a length that is the same as the diameter of the cuvette rim. When the reaction cuvette <NUM> is moved, the cuvette rim of the reaction cuvette <NUM> is engaged in the limit groove, so that the reaction cuvette <NUM> moves with the movement of the reaction cuvette claw <NUM>. Since the reaction cuvette claw <NUM> moves to the cuvette rim of the reaction cuvette when the electromagnet is powered on, the reaction cuvette <NUM> only moves horizontally in the slide <NUM> along with the reaction cuvette claw <NUM>, rather than moving vertically, dropping of the reaction cuvette does not occur. Moreover, the reaction cuvette claw has a simple structure, and is easy to assemble and process.

Preferably, the cuvette holding device and the slide rail <NUM> are set to be perpendicular to each other. An idler wheel <NUM> and a synchronous belt wheel <NUM> are respectively set at two ends of the slide rail <NUM> above the slide rail <NUM>, and the idler wheel <NUM> and the synchronous belt wheel <NUM> are connected together via a first synchronous belt <NUM>. The cuvette holding device is vertically connected to the first synchronous belt <NUM> and the first synchronous belt <NUM> is driven to rotate by a stepper motor <NUM>. When the first synchronous belt <NUM> rotates, the cuvette holding device is driven to move along the slide rail <NUM> and between the cuvette sorting module <NUM> and the incubation disk module.

Preferably, the slide <NUM> locates below the reaction cuvette claw <NUM>, and is parallel to the slide rail <NUM>. The reaction cuvette claw <NUM> drives, by means of induction of the electromagnet <NUM>, the reaction cuvette <NUM> to pass through the slide <NUM> and arrive at the incubation disk module. The reaction cuvette <NUM>, which is sorted by the cuvette sorting module <NUM>, locates at one end of the slide rail <NUM>, and at this time, the electromagnet <NUM> is started. After sensing starting of the electromagnet <NUM>, the first photoelectric sensor <NUM> drives a reaction cuvette claw <NUM> to move downward, and then an opening at the bottom of the reaction cuvette claw <NUM> engages the cuvette rim of the reaction cuvette <NUM>. Meanwhile, the stepper motor <NUM> drives the first synchronous belt <NUM> to rotate so as to drive a reaction cuvette moving device to move along the slide rail <NUM> toward the first incubation disk <NUM>. The reaction cuvette <NUM> passes through the slide <NUM>, and arrives at the first incubation disk <NUM>.

Preferably, the reset device <NUM> is a second photoelectric induction sensor including an N-shaped groove which allows the cuvette holding device to pass through. After the electromagnet <NUM> is powered off, the reaction cuvette claw <NUM> moves upward until the first photoelectric sensor <NUM> detects that the reaction cuvette claw <NUM> passes through a U-shaped groove of the first photoelectric sensor. The reaction cuvette claw <NUM> is absorbed on the electromagnet <NUM> and is kept away from the reaction cuvette <NUM>. At this time, the stepper motor <NUM> moves so as to drive the synchronous belt wheel <NUM> to rotate in a direction opposite to a direction of rotation when the reaction cuvette is delivered, and the first synchronous belt <NUM> drives the cuvette holding device to move in a direction opposite to a direction of rotation when the reaction cuvette is delivered. A process from delivering the reaction cuvette to resetting is completed when the cuvette holding device returns to the N-shaped groove of the reset device <NUM> which is the second photoelectric sensor, i.e., the cuvette holding device returns to the other end of the incubation disk.

By means of the cuvette delivering module <NUM>, the reaction cuvette <NUM> is capable of moving horizontally directly from the cuvette sorting module <NUM>, through the slide <NUM>, to the first incubation disk <NUM>, so that problems such as ahigh error rate, cuvette dropping and low working efficiency caused when a three-dimensional mechanical arm gripper to grab the reaction cuvette <NUM> are avoided. In addition, the motor is a stepper motor, so that the belt wheel operates more stably when a belt transmission device is controlled for transmission.

In an embodiment, as shown in <FIG>, the sample loading module <NUM> includes several test tube racks <NUM> and a moving device, which are set on a baseplate of the sample loading module <NUM>. The test tube racks <NUM> are moved to specified positions by means of the moving device.

In an embodiment, the moving device includes an X-axis pushing mechanism. The X-axis pushing mechanism includes a circulation pushing member <NUM>, which actuates the operation of a further synchronous belt by means of a motor.

In an embodiment, blocking pieces are provided with intervals on the further synchronous belt along the direction of the further synchronous belt. The circulation pushing member <NUM> pushes the test tube racks forward section by section by means of the further synchronous belt with the blocking pieces. The circulation pushing member <NUM> may synchronously push two test tube racks in two adjacent rows to move at the same time, so that one test tube is sampled while the other test tube is scanned, which can greatly improve the detection efficiency of the light initiated chemiluminescence detector.

In an embodiment, the moving device further includes a Y-axis pushing mechanism. The Y-axis pushing mechanism is a conveyor device. The conveyor device is driven by a motor, and conveys the test tube rack <NUM> to the circulation pushing member <NUM>.

In an embodiment, the X-axis pushing mechanism further includes a recycling pushing member <NUM> to recycle a sampled test tube.

Preferably, the recycling pushing member <NUM> locates opposite to the circulation pushing member <NUM>. The moving direction of the test tube rack pushed by the recycling pushing member <NUM> is counter to the direction of the test tube rack pushed by the circulation pushing member <NUM>.

In an embodiment, the conveyor device includes a first conveyor belt <NUM> and a second conveyor belt <NUM>, conveying directions of which are counter to each other. The second conveyor belt <NUM> conveys the test tube rack <NUM> to the circulation pushing member <NUM>, and the sampled test tube rack <NUM> is conveyed to the recycling pushing member <NUM> by the first conveyor belt <NUM>.

In an embodiment, the sample loading module <NUM> further includes a test tube barcode scanner <NUM>, a test tube type discriminator <NUM> and a first positioning device 104to determine a position for sampling a test tube. A scanning direction of the test tube barcode scanner <NUM> and a discrimination direction of the test tube type discriminator <NUM> face the test tube rack. The test tube barcode scanner <NUM> and the test tube type discriminator <NUM> are respectively used to collect barcode information of the test tube and discriminating a type of the test tube, so that relevant information is recorded, which ensures that detection is performed orderly. The first positioning device <NUM> positions a sampling position for the test tube to be sampled, so as to ensure that the test tube to be sampled arrives at a sampling position accurately. After sample barcode scanning and the test tube type discrimination to the test tube on the test tube rack <NUM> are completed, each sample is pushed to a sampling position.

Preferably, the test tube rack <NUM> is conveyed to circulation pushing member <NUM> by the second conveyor belt <NUM>, and circulation pushing member <NUM> pushes the test tube rack <NUM> forward. When the test tube rack <NUM> passes by the test tube barcode scanner <NUM> and the test tube type discriminator <NUM>, barcode scanning and type discriminating are performed automatically. Then the test tube rack <NUM> moves on, and when the first positioning device <NUM> senses that the test tube rack arrives at the sampling position, sampling is performed by the sampling arm module <NUM>. After the sampling is finished, the test tube rack <NUM> moves on and arrives at the first conveyor belt <NUM>, and then the test tube rack <NUM> is pushed toward the second conveyor belt <NUM> by the recycling pushing member <NUM>.

In an embodiment, the test tube type discriminator <NUM> is provided with a first sensor and a second sensor. The first sensor locates above the second sensor. The first sensor and the second sensor are both provided with a detection rod which extends toward the test tube rack. The detection rod of the first sensor has a length that is greater than a length of the detection rod of the second sensor. There are two types of test tubes on the test tube rack <NUM>, i.e., a test tube having a diameter of <NUM> and a test tube having a diameter of <NUM>. Since the detection rod of the first sensor has a length that is greater than a length of the detection rod of the second sensor, when the test tube having a diameter of <NUM> passes by the test tube type discriminator <NUM>, both the detection rod of the first sensor and the detection rod of the second sensor are capable of touching the test tube, and two sensor signals are sensed at this time; however, when the test tube having a diameter of <NUM> passes by the test tube type discriminator <NUM>, since the detection rod of the second sensor, i.e., the shorter detection rod is incapable of touching the test tube, only the sensor signal of the longer detection rod, i.e., the first sensor, is sensed at this time. Thus, based on the number of the sensed sensor signal, it is determined whether the type of the test tube passing by is the test tube having a diameter of <NUM> or the test tube having a diameter of <NUM>.

The sample loading module in the present disclosure is a rail sample loading module, so that a sample to be detected can be added randomly without stopping the detection, which improves the detection efficiency. Moreover, the test tub racks in two adjacent rows can be pushed forward synchronously, so that sampling is performed to one test tube while barcode scanning is performed to a next tube, which improves the speed of sample loading and thereby improves the speed of sample detection.

As shown in <FIG>, in an embodiment, the sampling arm module <NUM> includes a frame and an arm assembly. The arm assembly is set at an upper portion of the frame. A vertical motion assembly <NUM> and a rotary motion assembly <NUM> are provided on the frame. The vertical motion assembly <NUM> enables the vertical movement of the arm assembly vertically relative to the frame, and the rotary motion assembly <NUM> enables the rotation of the arm assembly relative to the frame.

The arm assembly includes a connecting arm <NUM>. One end of the connecting arm <NUM> is fixedly connected to a sample needle <NUM> which is perpendicular to the connecting arm <NUM>, and the other end of the connecting arm <NUM> is fixedly connected to a spline shaft <NUM> which is perpendicular to the connecting arm <NUM>. The spline shaft <NUM> may transfer motion in a linear direction, and may also transfer torque in a circumferential direction. The sample needle <NUM> is fixedly connected with the spline shaft <NUM> via the connecting arm <NUM>, so that the sample needle <NUM> is capable of moving with the vertical movement or rotary movement of the spline shaft <NUM>.

In an embodiment, the vertical motion assembly <NUM> includes a first motor <NUM>; the first motor <NUM> is connected to a first driving wheel <NUM>; the first driving wheel <NUM> is connected to a first driven wheel via a second synchronous belt <NUM>; a moving block <NUM> is fixed on the second synchronous belt <NUM>; and a lower end of the spline shaft <NUM> extends through the moving block <NUM>. When the first motor <NUM> is started, the first motor <NUM> drives the first driving wheel <NUM> to rotate so as to drive the second synchronous belt <NUM> to rotate; the moving block <NUM> moves vertically with rotation of the second synchronous belt <NUM>; and the spline shaft <NUM> moves with vertical movement of the moving block <NUM> so as to drive the sample needle <NUM>, which is fixedly connected with the spline shaft <NUM> via the connecting arm <NUM>, to move vertically.

Preferably, blocking rings are provided at both an upper end and a lower end of the moving block <NUM> to prevent the spline shaft <NUM> from moving vertically relative to the moving block.

In an embodiment, the rotary motion assembly <NUM> includes a second motor <NUM>; the second motor <NUM> is connected to a second driving wheel; the second driving wheel is connected to a second driven wheel 311via a third synchronous belt; a rotating block is provided at an upper portion of the second driven wheel <NUM>; the spline shaft <NUM> is sheathed by both the second driven wheel and the rotating block <NUM>. When the second motor <NUM> is started, the second motor <NUM> drives the third synchronous belt to rotate, and the third synchronous belt drives the second driven wheel <NUM> to rotate. The second driven wheel <NUM> rotates so as to drive the spline shaft <NUM> to rotate. Since the spline shaft <NUM> is capable of rotating inside the rotating block <NUM> relatively, the spline shaft <NUM> moves with rotation of the second driven wheel <NUM> so as to drive the sample needle <NUM>, which is fixedly connected with the spline shaft <NUM> via the connecting arm <NUM>, to rotate.

Preferably, a flat key is set between the second driven wheel <NUM> and the spline shaft <NUM> so as to prevent the spline shaft <NUM> from rotating relative to the second driven wheel <NUM>.

The sample needle <NUM> of the sampling arm module <NUM> is capable of moving with the vertical movement or rotation of the spline shaft <NUM>. This structure enables the sample needle <NUM> to load a sample or a reagent in different positions; since there is only one stage of rotation, i.e., the rotation of the spline shaft, this structure is small, simple, convenient to assemble and maintain, and low in cost; and since a combination of the rotation and the vertical movement is used, a motion trail of the sample needle <NUM> is definite, so that a high speed, a low error rate and a high precision can be achieved.

As shown in <FIG>, in an embodiment, the incubation disk module includes a first incubation disk <NUM> and a second incubation disk <NUM>, and each of the incubation disks is driven to rotate by a motor. A cuvette removing and discarding module 14is provided between the first incubation disk <NUM> and the second incubation disk <NUM>. The cuvette removing and discarding module <NUM> includes a cuvette pushing rail and a cuvette discarding rail, and the cuvette pushing rail and the cuvette discarding rail are switched by means of a linear motor. When an incubation time for a reaction cuvette in the first incubation disk <NUM> is up, the reaction cuvette <NUM> is rotated to a reaction cuvette removing position of the first incubation disk <NUM>. Meanwhile, a reaction cuvette in the second incubation disk <NUM> is also rotated to the reaction cuvette removing position. At this time, the cuvette removing and discarding module <NUM> is started and switches a rail to the cuvette discarding rail, so as to discard the reaction cuvette in the second incubation disk <NUM>; and then the cuvette removing and discarding module <NUM> switches the rail to the cuvette pushing rail, so as to push a detection reaction cuvette in the first incubation disk <NUM> to the second incubation disk <NUM>.

As shown in <FIG>, in an embodiment, the reagent disk module includes an open device <NUM> and a first rotating part <NUM> which is set at the bottom of the open device <NUM> and drives the open device <NUM> to rotate. The side wall of the open device <NUM> is provided with a scanning part <NUM>, and a scanning device is capable of identifying an attribute of a reagent inside the open device <NUM> by means of the scanning part <NUM>. Preferably, open device <NUM> is a reagent compartment.

Specifically, the scanning part <NUM> is a through hole which is set in a side surface of the open device <NUM>. The reagent inside the open device <NUM> is set in a test container <NUM>, and a label on the test container <NUM> can be seen via the through hole. The test container <NUM> may be a reagent bottle, a test tube, a beaker or a flask.

Further, the scanning part <NUM> is at the same level with the label on the test container <NUM> to facilitate scanning of the label on the test container <NUM> by the scanning device, such as a card reader or a scanner, so that a step of manual scanning and identifying by an operator is avoided, which reduces the risk of errors.

In addition, the scanning device transmits scanned information of the test container <NUM> at a current position (i.e., a reagent suctioning port) to a controller. The controller determines whether a reagent in the test container <NUM> at the current position is a desired reagent. If the reagent is the desired reagent, a reagent arm operates; and if the reagent is not the desired reagent, the first rotating part <NUM> is driven to rotate so as to rotate a test container <NUM> at a next position to the current position, and the above process is repeated. Therefore, by means of the above automatic scanning function, consistency of results can be maintained.

Further, a fixing device is provided in the reagent compartment. The test container <NUM> is set on the fixing device, and the fixing device and the test container <NUM> rotate along with the reagent compartment.

In an embodiment, a rotation connecting part is provided at the bottom of the reagent compartment. The rotation connecting part is connected to the first rotating part <NUM>, and the fixing device is fixedly connected to the rotation connecting part. The rotation connecting part is a swivel plate, and a center of the bottom of the swivel plate is connected to the first rotating part <NUM>.

In an embodiment, a second positioning device <NUM> to read a position of the reagent compartment is provided on the first rotating part. Specifically, the second positioning device <NUM> includes a sensor <NUM> which is fixed on a support body <NUM> and a sensor light-blocking sheet <NUM> which is set at the bottom of a rotation shaft <NUM>. An initial position of the swivel plate can be determined by the sensor <NUM> and the sensor light-blocking sheet <NUM>, and a desired test container can be positioned to the current position based on the initial position.

Specifically, the support body <NUM> is in a shape of a flat board. A synchronous belt together with a big synchronous wheel and a small synchronous wheel connected to the synchronous belt are respectively provided at an upper portion of the support body <NUM>. The big synchronous wheel is connected to the rotation shaft <NUM>, and the small synchronous wheel is driven by a motor. Certainly, the rotation shaft <NUM> can be connected to the motor via a gear mechanism or a chain mechanism, and details are not repeated herein.

Specifically, the sensor <NUM> includes a zero-position sensor and a position sensor set below the zero-position sensor. The sensor light-blocking sheet <NUM> includes a zero-position light-blocking sheet and a coded disk, and the zero-position light-blocking sheet and the coded disk are respectively fixed at an upper end and a lower end of the big synchronous wheel. The zero-position light-blocking sheet, when rotating with the rotation shaft <NUM>, passes by the zero-position sensor intermittently, and can calibrate a zero position of rotation. Multiple grooves are provided at equal angles on the coded disk, and each of the grooves corresponds to one test container. When the coded disk rotates with the rotation shaft, different grooves pass by the position sensor, so that position information of the test container can be obtained.

Preferably, a refrigeration device <NUM> is set outside the open device <NUM>, and the bottom of the refrigeration device <NUM> is connected to the first rotating part <NUM>. A rotation gap required for relative rotation is formed between the refrigeration device <NUM> and the open device <NUM>. The reagent disk module is provided with a refrigeration device, so that it is unnecessary to take out an unspent reagent and store it in another device, and the unspent reagent can be directly stored in the detector until the reagent is used up. Besides, there is a great amount of water evaporation when the reagent is positioned on an analyzer in the existing technologies, which affects the detection result. An instrument system of the present disclosure ensures a small amount of evaporation for the reagent.

In an embodiment, fixation seats <NUM> is provided in the reagent compartment, and each of the fixation seats <NUM> fixes one of the above test containers <NUM>. The first rotating part <NUM> drives the reagent compartment and the test containers <NUM> inside the reagent compartment to rotate together to make a test container <NUM> arrive at the reagent suctioning port, so that the reagent arm sucks the reagent in the test container <NUM>.

In an embodiment, the reagent in a reagent disk is transferred to the reaction cuvette by means of fluid system7 under the control of the reagent arm module. As shown in <FIG>, the fluid system <NUM> includes: a dispensing and rinsing system <NUM>, which dispenses the sample and the reagent and rinses the sample needle <NUM> and a reagent needle <NUM>;.

In an embodiment, the dispensing and rinsing system <NUM> includes: a sample allocating unit to allocate the sample; a reagent allocating unit to allocate the reagent; an inner rinsing unit to rinse the inner wall of the sample needle <NUM> and the inner wall of the reagent needle <NUM>; and an outer rinsing unit to rinse the outer wall of the sample needle <NUM> and the outer wall of the reagent needle <NUM>.

In an embodiment, the dispensing and rinsing system <NUM> further includes a water supplying unit to supply water for rinsing to the inner rinsing unit and the outer rinsing unit.

In an embodiment, the sample allocating unit includes a sample needle dispensing pump <NUM> and a sample needle <NUM>, and an outlet of the sample needle dispensing pump <NUM> is connected to an inlet of the sample needle <NUM>.

In an embodiment, the reagent allocating unit includes a reagent needle dispensing pump <NUM> and a reagent needle <NUM>, and an outlet of the reagent needle dispensing pump <NUM> is connected to an inlet of the reagent needle <NUM>. It is understandable that there may be one or more reagent allocating units, and three reagent allocating units are disclosed in the present embodiment. As shown in <FIG>, two reagent allocating units (in an area circled in <FIG>) allocate the reagent, and another reagent allocating unit (in the area circled in <FIG>) allocates a general-purpose liquid.

The inner rinsing unit includes a sample needle internal rinsing water pump <NUM> and a reagent needle internal rinsing water pump <NUM>. An inlet of the sample needle internal rinsing water pump <NUM> and an inlet of the reagent needle internal rinsing water pump <NUM> are both connected to an outlet of the water supplying unit. An outlet of the sample needle internal rinsing water pump <NUM> is connected to a first solenoid valve <NUM>, and the first solenoid valve <NUM> is connected to an inlet of the sample needle dispensing pump <NUM>. An outlet of the reagent needle internal rinsing water pump <NUM> is connected to a second solenoid valve <NUM>, and the second solenoid valve <NUM> is connected to an inlet of the reagent needle dispensing pump <NUM>.

In an embodiment, the acid cleaning system <NUM> includes a sample needle cleaning tank <NUM> and reagent needle cleaning tanks <NUM>. An inlet of the sample needle cleaning tank <NUM> and an inlet of the reagent needle cleaning tanks <NUM> are respectively connected to each of outlets of acid cleaning liquid pumps <NUM>. Inlets of the acid cleaning liquid pumps <NUM> are respectively connected to first check valves <NUM>, and the first check valves <NUM> are all connected to an acid cleaning liquid bottle <NUM>. An outlet of the sample needle cleaning tank <NUM> and an outlet of the reagent needle cleaning tanks <NUM> are respectively connected to the waste discharging system <NUM>.

During water rinsing, high-pressure water passes through the sample needle dispensing pump <NUM> and flows out of an inner space of the sample needle <NUM>, so as to rinse the inner wall of the sample needle <NUM>; and water flowing out of the sample needle <NUM> passes through a sample needle cleaning tank <NUM> and further cleans the outer wall of the sample needle <NUM>. During internal water rinsing of the reagent needle <NUM>, the high-pressure water passes through the reagent needle dispensing pump <NUM> and flows out of the reagent needle <NUM>, so as to rinse the inner wall of the reagent needle <NUM>; and water flowing out of the reagent needle <NUM> passes through a reagent needle cleaning tank <NUM> and further cleans the outer wall of reagent needle <NUM>. However, the sample needle <NUM> has a small inner diameter, a water flow going out of the inner space of the sample needle 301per unit time is not sufficient to clean the sample needle <NUM> thoroughly. Thus, a sample needle external rinsing water pump <NUM> is added to increase the water flow in the sample needle cleaning tank <NUM>, so as to clean the sample needle <NUM> thoroughly. The reagent needle <NUM> has a larger inner diameter, and water flow per unit time used in internal rinsing only is sufficient to clean reagent needle <NUM> thoroughly.

During performing of acid cleaning, a cleaning liquid passes through an acid cleaning liquid pump <NUM> and flows into the sample needle cleaning tank <NUM>, and the sample needle 301sucks and discharges the cleaning liquid repeatedly for <NUM> times in the sample needle cleaning tank <NUM>, so as to achieve the purpose of pickling the sample needle <NUM>. When the reagent needle is pickled, the cleaning liquid passes through the acid cleaning liquid pump <NUM> and flows into the reagent needle cleaning tank <NUM>, and the reagent needle sucks and discharges the cleaning liquid repeatedly for <NUM> times in reagent needle cleaning tank <NUM>, so as to achieve the purpose of pickling the reagent needle <NUM>.

In an embodiment, the waste discharging system <NUM> includes a negative pressure tank44. A liquid inlet of the negative pressure tank44 is connected to the acid cleaning system <NUM>. A liquid outlet of the negative pressure tank44 is connected to an inlet of a waste liquid discharging pump <NUM> via a third solenoid valve <NUM>. An outlet of the waste liquid discharging pump <NUM> is connected to an inlet of a waste liquid bottle. An air outlet of the negative pressure tank44 is connected to an inlet of the negative pressure pump <NUM>. A second check valve20 is set between the air outlet of the negative pressure tank <NUM> and the inlet of negative pressure pump <NUM>. An outlet of the waste liquid discharging pump <NUM> is merged with a fourth branch A0. A third check valve <NUM> is set upstream from a position where the waste liquid discharging pump <NUM> is merged with the fourth branch A0, so that when a flow in the waste liquid discharging pump <NUM> is overly large, the third check valve <NUM> is capable of preventing the waste liquid from flowing back.

Working processes of respective sections of the fluid system are described respectively below.

During dispersing of the sample, the first solenoid valve <NUM> is closed, and sample needle dispensing pump is driven by a motor to suck and disperse the sample by means of the sample needle <NUM>.

During dispersing of the reagent, the second solenoid valve <NUM> is closed, and the reagent needle dispensing pump is driven by a motor to suck and disperse the reagent by means of the reagent needle <NUM>. It is understandable that if it is required to disperse the general-purpose liquid, one of the reagent dispersing units disperses the general-purpose liquid. Likewise, the second solenoid valve <NUM> is closed, a general-purpose liquid needle dispensing pump is driven by a motor to suck and disperse the general-purpose liquid by means of a general-purpose liquid needle.

When a water level in a clean water tank <NUM> reaches a first water level, a water inlet valve <NUM> is opened, and a water inlet pump <NUM> is turned on to take in water; and when the water level in the clean water tank <NUM> rises to a second water level, the water inlet pump <NUM> is turned off to stop taking in water, and the water inlet valve <NUM> is closed. When the water level in the clean water tank <NUM> rises to a third water level, a water level alarm is given, and it is required to check by a human. During internal rinsing of the sample needle, the first solenoid valve <NUM> is opened, and the sample needle internal rinsing water pump <NUM> is turned on; the high-pressure water passes through the sample needle dispensing pump <NUM> and flows out of the inner space of the sample needle <NUM> so as to rinse the inner wall of the sample needle <NUM>; and water following out of the sample needle <NUM> passes through the sample needle cleaning tank <NUM> and further rinses the outer wall of the sample needle <NUM>. During internal rinsing of the reagent needle <NUM>, the second solenoid valve <NUM> is opened, and the reagent needle internal rinsing water pump <NUM> is turned on, and the high-pressure water passes through the reagent needle dispensing pump <NUM> and flows out of the inner space of the reagent needle <NUM> so as to rinse the inner wall of the reagent needle <NUM>; and water flowing out of the reagent needle <NUM> passes through the reagent needle cleaning tank <NUM> and further rinses the outer wall of the reagent needle <NUM>.

During external rinsing of the sample needle <NUM>, the sample needle external rinsing water pump <NUM> is started, and the high-pressure water flows into the sample needle cleaning tank <NUM>. The sample needle <NUM> sucks and discharges the water repeatedly in the sample needle cleaning tank <NUM>, so as to clean the inner wall and the outer wall of the sample needle 301thoroughly.

When a liquid level of an acid cleaning liquid in the acid cleaning liquid bottle is lower than a liquid level of a second liquid level sensor <NUM>, the second liquid level sensor <NUM> will give an alarm to remind that it is required to add the acid cleaning liquid; and after the acid cleaning liquid is added, the alarm about the liquid level is canceled automatically. When the sample needle <NUM> is pickled, the corresponding acid cleaning liquid pump <NUM> is turned on. The cleaning liquid passes through the acid cleaning liquid pump <NUM> and flows into the sample needle cleaning tank <NUM>, and the sample needle <NUM> sucks and discharges the cleaning liquid repeatedly for <NUM> times in the sample needle cleaning tank <NUM>, so as to achieve the purpose of pickling the sample needle <NUM>. When the reagent needle is pickled, the corresponding acid cleaning liquid pump <NUM> is turned on. The cleaning liquid passes through the acid cleaning liquid pump <NUM> and flows into the reagent needle cleaning tank <NUM>, and the reagent needle sucks and discharges the cleaning liquid repeatedly for <NUM> times in reagent needle cleaning tank <NUM>, so as to achieve the purpose of pickling the reagent needle <NUM>.

When an air pressure in the negative pressure tank <NUM> is higher than -<NUM>/m<NUM>, the negative pressure pump <NUM> is turned on. Air flows out through a silencer <NUM>, and when the air pressure in the negative pressure tank <NUM> is lower than -<NUM>/m<NUM>, the negative pressure pump <NUM> is turned off. When a liquid level of the waste liquid is higher than an alarm liquid level of a third liquid level sensor <NUM>, the waste liquid discharging pump <NUM> is turned on, and the third solenoid valve <NUM> is opened so as to start to discharge the waste liquid. When the liquid level of the waste liquid is lower than the alarm liquid level, the third solenoid valve <NUM> is closed, and the waste liquid discharging pump <NUM> is turned off. The third solenoid valve <NUM> is opened <NUM> later after water rinsing is performed in the reagent needle cleaning tank <NUM> and the sample needle cleaning tank <NUM>, and discharging of waste water produced by rinsing starts; and after the rinsing is finished, the third solenoid valve <NUM> is closed. The third solenoid valve <NUM> is opened <NUM> later after acid cleaning is performed in the reagent needle cleaning tank <NUM> and the sample needle cleaning tank <NUM>, and discharging of cleaning liquid waste water produced by acid cleaning starts; and after the acid cleaning is finished, the third solenoid valve <NUM> is closed.

By disposing a dispensing and rinsing system, an acid cleaning system and a waste discharging system, the fluid system of the present disclosure realizes multiple functions, such as storage of a sample liquid, sample loading, and collection of a waste liquid, and has high degree of automation; and moreover the fluid system has functions of real-time monitoring and automatic protection, so that safety and reliability of the system can be improved.

In an embodiment, the detection module includes a light path detection system <NUM> and a control system <NUM>. As shown in <FIG>, the light path detection system <NUM> includes an excitation unit <NUM>, an excitation light pathway switch <NUM>, a signal light pathway switch124 and a detection member <NUM>. The excitation light pathway switch <NUM> is used to control conduction or blocking of an excitation light emitted by the excitation unit <NUM>. The excitation light pathway switch <NUM> is connected to the signal light pathway switch <NUM> via a second actuating part <NUM>. The second actuating part <NUM> controls the excitation light pathway switch <NUM> and the signal light pathway switch <NUM> to move synchronously in opposite directions, and is capable of controlling opening and closing of the excitation light pathway and the signal light pathway at the same time. When the excitation light pathway is opened, the signal light pathway is closed; and when the excitation light pathway is closed, the signal light pathway is opened.

When it is required to excite a substance to be detected by the excitation light, the second actuating part <NUM> rotates and actuates the excitation light pathway switch <NUM> to rotate, so that the excitation light pathway is opened (as shown in <FIG>); and meanwhile the second actuating part <NUM> actuates the signal light pathway switch <NUM> to rotate, so that the signal light pathway switch <NUM> in a closed state (as shown in <FIG>). Likewise, when it is required to receive and detect luminescence signals generated by the substance to be detected, the second actuating part <NUM> rotates again and actuates the excitation light pathway switch <NUM> to rotate, so that the excitation light pathway switch <NUM> blocks the excitation light (as shown in <FIG>); and meanwhile the second actuating part <NUM> actuates the signal light pathway switch <NUM> to rotate, so that the signal light pathway switch <NUM> is in an opened state (as shown in <FIG>). The second actuating part <NUM> controls opening and closing of the excitation light pathway and the signal light pathway at the same time. When the excitation light pathway is opened, the signal light pathway is closed; and when the excitation light pathway is closed, the signal light pathway is opened.

In an embodiment, output shafts are respectively provided at two ends of the second actuating part <NUM>.

In a preferred embodiment, the second actuating part <NUM> is a rotating electromagnet.

In an embodiment, as shown in <FIG> and <FIG>, the excitation light pathway switch <NUM> includes a second rotating part <NUM>. Through holes <NUM> are provided at a circumferential side wall of a first end of the second rotating part <NUM>, which through holes are used to realize conduction of the excitation light, and a second end of the second rotating part <NUM> is fastened to output shaft of a first end of the second actuating part <NUM>. When it is required to control the excitation light to excite the substance to be detected, the second actuating part <NUM> actuates the second rotating part <NUM> to rotate, and the excitation light pathway switch <NUM> opens the excitation light pathway, so that the excitation light goes through the through holes <NUM> (as shown in <FIG>). When it is required to control the excitation light to be blocked, the second actuating part <NUM> actuates the second rotating part <NUM> to rotate again, so that the excitation light pathway switch <NUM> is capable of preventing the excitation light from going through the through holes <NUM> (as shown in <FIG>).

In a preferred embodiment, there are two through holes <NUM>, and the two through holes <NUM> at the circumferential side wall of the first end of the second rotating part <NUM> are opposite to each other.

Preferably, the second rotating part <NUM> is in a shape of a tube.

In a preferred embodiment, the excitation unit <NUM> includes a first housing base <NUM>. The first end of the second rotating part <NUM> extends through one side wall of the first housing base <NUM> into the interior of the first housing base <NUM>. The two through holes <NUM> are set inside the first housing base <NUM>. The circumferential side wall of the second rotating part <NUM> is rotatably connected to one side wall of the first housing base <NUM>. The top and the bottom of the first housing base <NUM> are both provided with an excitation light channel <NUM>. When it is required to control the excitation light to excite the substance to be detected, the second actuating part <NUM> rotates, and the output shaft of a first end of the second actuating part <NUM> actuates the second rotating part <NUM> to rotate, so that the two through holes <NUM> are aligned with the two excitation light channels <NUM> (as shown in <FIG>). The excitation light emitted by the excitation unit <NUM> goes through the two excitation light channels <NUM> to excite the substance to be detected. At this time, the signal light pathway switch <NUM> is in the closed state (as shown in <FIG>).

In a preferred embodiment, the light path detection system <NUM> further includes a light path component, and the light path component is set below the excitation unit <NUM>.

In an embodiment, as shown in <FIG>, a transflective lens <NUM>, which transmits the excitation light and reflects the luminescence signals generated by the substance to be detected after the excitation with the excitation light, is set at the side of the second rotating part <NUM> and away from the excitation unit <NUM>. The transflective lens <NUM> is capable of not only transmitting the excitation light having a target wavelength and blocking the excitation light not having a target wavelength but also reflecting the luminescence signals having a target wavelength generated by the substance to be detected.

A first lens <NUM>, which focuses the luminescence signals generated by the substance to be detected, is set at the side of the transflective lens <NUM>, and the first lens <NUM> is close to the signal light pathway switch <NUM>. The luminescence signals generated by the substance to be detected that is reflected by the transflective lens <NUM> is capable of passing through the first lens <NUM> and enters the detection member <NUM>.

In a preferred embodiment, an optical filter <NUM> is provided at the side of the first lens <NUM> close to the signal light pathway switch <NUM>. The luminescence signals generated by the substance to be detected, after being reflected by the transflective lens <NUM>, pass through the first lens <NUM> and the optical filter <NUM> sequentially and enter the detection member <NUM>. The optical filter <NUM> is capable of extracting signals having a desired wavelength in the luminescence signals generated by the substance to be detected and blocks stray light signals having a wavelength other than the desired wavelength.

In a preferred embodiment, as shown in <FIG>, the transflective lens <NUM>, the first lens <NUM> and the optical filter <NUM> are all set inside a second housing base <NUM>. The top of the second housing base <NUM> is fastened to the bottom of the first housing base <NUM>. A first opening is provided on one side wall of the second housing base <NUM>, which first opening transmits the luminescence signals generated by the substance to be detected and leads to the signal light pathway switch <NUM>. A second opening <NUM> is provided at the bottom of the second housing base138, which second opening is set to match the substance to be detected.

In an embodiment, as shown in <FIG>, the excitation unit <NUM> includes a laser <NUM> to emit the excitation light. The excitation light emitted by the laser <NUM> excites, multiple times, the substance to be detected, such that the substance to be detected generates a plurality of luminescence signals.

In a preferred embodiment, as shown in <FIG>, a second lens <NUM> to focus the excitation light is set at a position between the laser <NUM> and the second rotating part <NUM>.

In a preferred embodiment, the laser <NUM> is set on a laser holder <NUM>. The second lens <NUM> is set on a second lens holder <NUM>. A top of the second lens holder <NUM> is fastened to the bottom of the laser holder <NUM>, and the bottom of the second lens holder <NUM> is fastened to the top of the first housing base <NUM>.

In the above embodiment, as shown in <FIG>, the signal light pathway switch <NUM> includes a baffle <NUM> and a crank linkage device <NUM>. The baffle <NUM> is fastened to the detection member <NUM>, and a third opening <NUM>, which corresponds to the first opening, is provided on the baffle <NUM>. The crank linkage device140 is set at one side of the baffle <NUM> close to the first opening.

In the above embodiment, as shown in <FIG> and <FIG>, the crank linkage device <NUM> includes a first rotating component <NUM>, which is fastened to output shaft of the second end of the second actuating part <NUM>, and a second rotating component <NUM>. A fourth opening <NUM> is set on the second rotating component145. The first rotating component <NUM> and the second rotating component <NUM> are both rotatably connected to the baffle <NUM>. When the output shaft of the second end of the second actuating part <NUM> actuates the first rotating component144to rotate anticlockwise around a rotation center thereof, the first rotating component <NUM> actuates the second rotating component <NUM> to rotate clockwise around a rotation center thereof, so that the fourth opening <NUM> is aligned with the third opening <NUM> (as shown in <FIG>). In this way, the third opening <NUM> is in communication with the detection member <NUM>, and the luminescence signals generated by the substance to be detected enter the detection member <NUM> for detection. At the same time, the output shaft of a first end of the second actuating part <NUM> actuates the second rotating part <NUM> to rotate anticlockwise so as to make the two through holes <NUM> and the two excitation light channels <NUM> staggered with each other (i.e., the two through holes <NUM> and the two excitation light channels <NUM> are not in communication with each other). At this time, the excitation light pathway switch <NUM> blocks the excitation light (as shown in <FIG>), so as to ensure that a process of detecting the luminescence signals generated by the substance to be detected and a process of exciting the substance to be detected with the excitation light do not interfere with each other, thereby improving accuracy of detection information.

In an embodiment, when the excitation light is conducted to excite the substance to be detected (as shown in <FIG>), the fourth opening <NUM> is staggered with the first opening and the third opening <NUM> (i.e., the fourth opening 143is not in communication with the first opening and the third opening <NUM>), and at this time, the signal light pathway switch <NUM> is in the closed state (as shown in <FIG>).

In the above embodiment, when the luminescence signals generated by the substance to be detected enter the detection member <NUM>, the first opening and the third opening <NUM> are aligned with the fourth opening <NUM> (i.e., the fourth opening <NUM> is aligned with the first opening and the third opening <NUM>), and the signal light pathway switch <NUM> is in the opened state (as shown in <FIG>). At this time, the excitation light pathway switch <NUM> blocks the excitation light (as shown in <FIG>).

In the above embodiment, the detection member <NUM> includes a detector, and the detector detects, multiple times, the luminescence signals generated by the substance to be detected and records corresponding results. The detector is a single photon counter, a photomultiplier tube or a silicon photocell.

In a preferred embodiment, the detector is the single photon counter.

In an embodiment, when the substance to be detected is a solution obtained after a chemiluminescence immunoreaction is performed, the excitation light emitted by the laser <NUM> in the excitation unit <NUM> may be used to excite, multiple times, the substance to be detected, so as to make the substance to be detected generate multiple chemiluminescence signals. The detection member <NUM> performs multiple times of collecting and obtains readings, converts the above chemiluminescence signals into digital signals for corresponding processing (a process of detecting the chemiluminescence signals by the detection member <NUM> includes collecting the chemiluminescence signals and obtaining the readings and then performing corresponding processing to the chemiluminescence signals), and records corresponding detection results, which can improve the detection efficiency and the accuracy of the detection information.

In a preferred embodiment, when the substance to be detected is the solution obtained after a chemiluminescence immunoreaction is performed, the excitation light emitted by the laser <NUM> in the excitation unit <NUM> is used to excite the substance to be detected twice, so as to generate two chemiluminescence signals, and the detector in the detection member <NUM> records the readings for the two chemiluminescence. After two readings are obtained, a processing unit processes the two readings. When an increasing range between the second reading and the first reading is larger than a maximum value of a standard curve, it can be determined that the immunoassay has a Hook risk. Based on the readings for the two chemiluminescence, an increasing range for a difference between the second reading and the first reading is indicated by A. Standard curves are respectively made based on first reading for a series of known standard substances containing a target antigen (or an antibody) and the increasing ranges A between two readings. The first reading for the substance to be detected containing the target antigen (or an antibody) and the increasing range A between two readings are compared with the standard curves, so that a concentration of the substance to be detected can be determined.

Light signals generated by exciting a sample detection liquid with the excitation light are transmitted to the detection member <NUM> via a light path member, and the detection member <NUM> converts the light signals into electric signals. The excitation light pathway switch and the signal light pathway switch of the present disclosure move synchronously in opposite directions, so as to ensure that a process of exciting a reactant in the reaction cuvette with the excitation light and a process of detecting chemiluminescence signals of the reactant do not interfere with each other, thereby improving accuracy of detection information for the chemiluminescence signals of the reactant and shortening a detection period. The present disclosure can also prevent problems of a time difference and hole-skipping detection in the detection process.

In an embodiment, the fully automatic light initiated chemiluminescence detector further includes an emergency position <NUM>. The emergency position <NUM> is provided thereon with a corresponding sensing device. When an emergency sample is placed at the emergency position <NUM>, the sensing device senses the emergency sample, and detection of the emergency sample will be performed first. At this time, the sampling arm module <NUM> temporarily stops sucking the sample from the test tube in the rail sample loading module <NUM>, but sucks a sample liquid from the emergency sample and transfers the sample liquid to the liquid cuvette <NUM>. After sucking of the emergency sample is finished, the sampling arm module <NUM> continues to suck the sample in an ordinary rail sample loading device.

In an embodiment, the fully automatic light initiated chemiluminescence detector further includes a sample diluent compartment <NUM>. There are at least two kinds of sample diluents in the sample diluent compartment <NUM>, and the sampling arm module <NUM> sucks the diluent to dilute the sample.

In an embodiment, the fully automatic light initiated chemiluminescence detector completes the detection through the following process. The rail sample loading module <NUM> pushes the sample to be detected to a sample sucking position. The cuvette sorting module <NUM> sorts the disordered reaction cuvettes <NUM> into ordered reaction cuvettes, and the cuvette delivering module <NUM> delivers the reaction cuvette 160to a cuvette feeding position of the first incubation disk <NUM>. Then, the first incubation disk <NUM> rotates, and brings the reaction cuvette <NUM> from the cuvette feeding position to a sample adding position. Subsequently, the sampling arm module <NUM> is controlled by the fluid system to rotate so as to suck the sample from the test tube arriving at the sample sucking position in the rail sample loading module <NUM>, and then the sampling arm module <NUM> rotates to the sample adding position of the first incubation disk <NUM> so as to add the sample into the reaction cuvette <NUM> at the sample adding position. If there is an emergency sample, the sampling arm module <NUM> will suck the emergency sample first, and continues previous sucking of the sample in the rail sample loading module <NUM> after sucking of the emergency sample is finished. After that, the sampling arm module <NUM> sucks the diluent to dilute the sample. Then, the first incubation disk <NUM> continues to rotate so as to rotate the reaction cuvette <NUM> at the sample addition position to a reagent position; and meanwhile the first reagent disk8 rotates so as to rotate the reagent thereon to a first reagent sucking position. The first reagent arm <NUM> is controlled by the fluid system to suck a certain amount of a first reagent from the reagent sucking position of the first reagent disk <NUM>, rotates to the reagent position of the first incubation disk <NUM>, and adds the first reagent into the reaction cuvette <NUM>.

After that, the first incubation disk <NUM> continues to rotate so as to rotate the reaction cuvette <NUM> from the first reagent position to a second reagent position; and meanwhile, the first reagent disk <NUM> rotates so as to rotate the reagent to a second reagent sucking position. The second reagent arm 15is controlled by the fluid system <NUM> to suck a certain amount of a second reagent from the reagent sucking position of the first reagent disk <NUM>, and rotates to the second reagent position of the first incubation disk <NUM> so as to add the second reagent into the reaction cuvette <NUM>; and the first incubation disk <NUM> rotates the reaction cuvette <NUM> containing a mixed solution to a mixing position. Then, a mixing mechanism mixes the sample in the reaction cuvette <NUM> uniformly. After that, the reaction cuvette <NUM> is rotated and incubated in the first incubation disk <NUM> for a certain period. After incubation time is up, the reaction cuvette <NUM> is rotated to a reaction cuvette removing position of the first incubation disk <NUM> exactly. Meanwhile, the second incubation disk <NUM> rotates and rotates to a reaction cuvette removing position; and the cuvette removing and discarding module <NUM> is started, and the rail is switched to the cuvette discarding rail so as to discard the reaction cuvette <NUM> on the second incubation disk <NUM>. Then, the rail is switched to the cuvette pushing rail for the cuvette removing and discarding module <NUM>, so that the reaction cuvette <NUM> to be detected in the first incubation disk <NUM> is removed to the second incubation disk <NUM>. The reaction cuvette <NUM> is rotated to a reagent adding position along with the second incubation disk <NUM>; meanwhile, the second reagent disk <NUM> rotates, and rotates the reagent to a third reagent sucking position of the second reagent disk <NUM>; and the third reagent arm <NUM> rotates and is controlled by the fluid system <NUM> to suck a third reagent from the reagent bottle, and then rotates to the reagent adding position of the second incubation disk so as to discharge the reagent into the reaction cuvette <NUM>. After that, the reaction cuvette <NUM> is rotated with the second incubation disk <NUM>. After incubation time is up, the reaction cuvette <NUM> is transferred to a light path detection system <NUM>, and the light path detection system <NUM> performs optical detection to the sample in the reaction cuvette <NUM>. The sample is excited by the excitation light emitted by the excitation unit to generate luminescence signals, and collecting the chemiluminescence signals and obtaining the readings are performed multiple times; the above chemiluminescence signals are converted into digital signals for corresponding processing (a process of detecting the chemiluminescence signals by the detection member <NUM> includes collecting the chemiluminescence signals and obtaining readings and then performing corresponding processing to the chemiluminescence signals), and then the processed digital signals are transmitted to the control system <NUM>, so that the control system <NUM> performs detection analysis to received information.

After that, the reaction cuvette <NUM> is rotated along with the second incubation disk <NUM>, and is rotated to a reagent reaction cuvette discarding and feeding position of the second incubation disk <NUM>. Meanwhile, the cuvette removing and discarding module <NUM> is started and switches the rail to the cuvette discarding rail, so as to push out and discard a detected reaction cuvette <NUM>. Now, the entire sample detection process is completed.

The entire process is operated automatically, so that problems, such as individual differences, manual errors, non-standard actions and other uncertainties, in the manual operation can be effectively avoided, and the accuracy of the chemiluminescence immunoassay can be improved.

Claim 1:
A fully automatic light initiated chemiluminescence detector, comprising a sample loading module (<NUM>), a sampling arm module (<NUM>), an incubation disk module, a reagent disk module, a reagent arm module and a detection module,
wherein a sample in the sample loading module is transferred to a reaction cuvette (<NUM>) by means of the sampling arm module (<NUM>); after the reaction cuvette (<NUM>) arrives at a specified position, the reagent arm module transfers a reagent in the reagent disk module to the reaction cuvette (<NUM>); the sample and the reagent in the reaction cuvette (<NUM>) are mixed to be incubated at the incubation disk by a mixing mechanism; and after the incubation, the detection module sends light on a substance to be detected in the reaction cuvette (<NUM>) to initiate a reaction between the sample and the reagent, and detects a luminescence signal generated after the initiation,
characterized in that the fully automatic light initiated chemiluminescence detector further comprises a cuvette sorting module (<NUM>) and a cuvette delivering module (<NUM>) located between the cuvette sorting module (<NUM>) and the incubation disk module, the cuvette sorting module (<NUM>) being configured to sort disordered reaction cuvettes into ordered reaction cuvettes, which are delivered to the incubation disk module in sequence via the cuvette delivering module (<NUM>), and
wherein the cuvette delivering module (<NUM>) comprises a slide rail (<NUM>), a cuvette holding device slidably connected to the slide rail (<NUM>), a transmission device for controlling a movement of the cuvette holding device along the slide rail (<NUM>), a slide channel (<NUM>) located below the cuvette holding device, and a reset device (<NUM>) enabling the reset of the cuvette holding device, the cuvette holding device being configured to control a movement of the reaction cuvette (<NUM>), so that the cuvette (<NUM>) is delivered horizontally directly from the cuvette sorting module (<NUM>) through the slide channel (<NUM>) to the incubation disk module.