Patent Description:
In some detection apparatuses, such as flow cytometers, the analytes (such as cells or particles) suspended in a liquid flow or a gas flow are arranged in a row and kept advancing sequentially in the center of a flow channel of the flow chamber. The light source irradiates the analytes which sequentially pass through the flow channel in a vertical direction, resulting in forward-angle scattered light and side-angle scattered light. If the analytes are marked with a fluorescent dye, it will also generate fluorescence signals which scatter to various angles in the space. The scattered light including fluorescent is collected and then converted into electrical signals through a photoelectric conversion device, and finally converted into digital signals that can be processed by computers which can be used to analyze the characteristics of the analytes.

Light energy collecting includes two steps of work, the first is to collect the light emitted by the analytes, and the second is to converge the collected light to convert it into the subsequent optical path for the photoelectric conversion process. Wherein, in the application field of flow cytometry, the fluorescence signal is the key information for analyzing the analytes, the more fluorescent light energy is collected and utilized, the higher the reliability of the signal and the more accurate the analysis, which is also beneficial to the identification and analysis of smaller-scale analytes. Therefore, improving the collection efficiency of the light collection optical system is the key to improving the reliability of the detection apparatus.

<CIT> is directed to a flow cytometer including a laser diode ("LD") based optical subsystem for impinging a beam of light upon particles passing through a viewing zone, a composite microscope objective for gathering and imaging light scattered from or fluoresced by particles passing through the viewing zone, a fluidic subsystem for supplying a liquid sheath flow to the viewing zone, a peristaltic pump for injecting into the liquid sheath flow a liquid sample flow carrying particles that passes together with the liquid sheath flow through the viewing zone, a multimode optical fiber that receives scattered and fluoresced light from the viewing zone that the composite microscope objective gathers and images, and a wavelength division multiplexer for optically separating into color bands light received via the optical fiber.

<CIT> is directed to a light collecting system and a cell analyzer. The light collecting system comprises a first planoconvex lens, a second planoconvex lens, a first double-bonding lens and a second double-bonding lens. The flat surface of the first planoconvex lens is a light incident face. The convex surface of the first planoconvex lens is a light outgoing face. The second planoconvex lens has a common optical axis with the first planoconvex lens. The flat surface of the second planoconvex lens is a light incident face. The convex surface of the second planoconvex lens is a light outgoing face. The first double-bonding lens has the common optical axis with the first planoconvex lens and the second planoconvex lens. The outer surface of a minus lens of the first double-bonding lens is a light incident face. The outer surface of a plus lens is a light outgoing face. The second double-bonding lens is located on a light path formed after light passes through the first planoconvex lens, the second planoconvex lens and the first double-bonding lens and is polymerized. The outer surface of a plus lens of the second double-bonding lens is a light incident face. The outer surface of a minus lens of the second double-bonding lens is a light outgoing face. Through combination of two planoconvex lenses and two double-bonding lenses, the light collecting system achieves larger numerical aperture, meets requirements for working distance, and reduces processing and assembling process difficulty.

<CIT> is directed to a flow cytometer fluorescence collection lens and an optical path system thereof, the lens realizing a large numerical aperture through the combination of a plano-convex lens, a positive meniscus lens, a biconvex lens and two doublet lenses, and can collect fluorescence scattering light in a half-angle range of <NUM> degrees; the lens meets the requirement that the working distance is larger than <NUM>, and can be compatible with a flow chamber used on a common flow cytometer; good chromatic aberration correction capability is realized on the wavelength range of <NUM> to <NUM>; parallel light can be output, the beam diameter is within <NUM>, the lens meets the use requirement that the dichroic mirror and the color filter are sensitive to the incident angle of the light beam, meanwhile, the color filter and the dichroic mirror achieve light beam transmission with the small size, the coating cost and the instrument cost are greatly saved, and the lens is simple, easy to obtain and suitable for common flow cytometry.

<CIT> is directed to a reflective fluorescence collection device for a flow cytometer, which comprises an objective lens group and a light splitting component consisting of a plano-convex reflection lens and a meniscus aspheric lens, and a forward collection component consisting of an aspheric lens, fluorescence collected by the objective lens group and the light splitting assembly is detected by a light splitting module composed of a spectroscope, a focus lens and a detector, a reflective fluorescence receiving structure is utilized, the NA1. <NUM>-<NUM> numerical aperture can be achieved only by using two lenses, and meanwhile the problem that a flow cytometer uses multiple laser spaces for space separation is solved.

However, the current light energy collecting system cannot solve the problems of correcting chromatic aberration, system complexity or poor reliability, and high cost at the same time.

Some embodiments of the present disclosure provide a light energy collecting system and a detection apparatus, to correct chromatic aberration, simplify system structure, and improve system reliability and stability.

In some embodiments, a light energy collecting system comprising: a flow chamber, comprising a first side and a second side opposite to each other; a reflector, glued on the first side of the flow chamber; and a cemented doublet, comprising a first outer side which is planar and cemented to the second side of the flow chamber; the cemented doublet comprising a negative focal power lens and a positive focal power lens cemented to each other, the refractive index of the negative focal power lens is greater than the refractive index of the positive focal power lens, and the Abbe number of the negative focal power lens is smaller than the Abbe number of the positive focal power lens.

The technical solutions of embodiments of the present disclosure have the following advantages: the light energy collecting system provided by some embodiments of the present disclosure, wherein a cemented doublet is arranged which comprises a negative focal power lens and a positive focal power lens cemented together, and the refractive index of the negative focal power lens is greater than that of the positive focal power lens, and the Abbe number of the negative focal power lens is smaller than the Abbe number of the positive focal power lens, thereby utilizing the dispersion properties of positive and negative focal power lenses with different refractive indices and Abbe numbers, the dispersion can be compensated for each other to achieve the purpose of correcting or eliminating chromatic aberration, which is beneficial to obtain a smaller focused spot and correspondingly improve the concentration of light energy.

Moreover, the first outer side of the cemented doublet is planar such that the first outer side of the cemented doublet can be fully glued to the second side of the flow chamber, which is beneficial to improve the bonding strength of the cemented doublet and the flow chamber, and correspondingly prevents the mis-alignment between the cemented doublet and the flow chamber during use, which affects the focusing effect, and prevents dust from entering the optical surface due to partial degumming and cracking on the edge which is difficult to remove, thereby improving the reliability of the light energy collecting system. In addition, the negative focal power lens and the positive focal power lens of the cemented doublet are cemented to each other, so that no additional structure is required for fixing and assembly, and there is no need to coat the first outer side of the cemented doublet and the second side of the flow chamber, which is beneficial to simplify the structure of light energy collecting system, reduce the cost, thereby improving the stability of the light energy collecting system.

In some embodiments, the cemented doublet further comprising a second outer side opposite to the first outer side, and the second outer side is aspheric.

In some embodiments, the cemented doublet further comprising a second outer side opposite to the first outer side, and the second outer side is aspheric, compared with the solution of correcting the aberration with only one aspherical lens, some embodiments of the present disclosure adds an additional lens (that is, the lens of which the outer side is used as the first outer side) glued with the aspheric lens, which is beneficial to further correct chromatic aberration, thereby helping obtain a smaller focused spot and correspondingly improving the concentration of light energy.

In some embodiments, the cemented surface between the negative focal power lens and the positive focal power lens is spherical.

In some embodiments, the negative focal power lens is a plano-concave lens, a plane of the negative focal power lens is configured as the first outer side of the cemented doublet, and a concave surface of the negative focal power lens is cemented with the positive focal power lens; the cemented doublet further comprising a second outer side opposite to the first outer side, a side of the positive focal power lens opposite to the negative focal power lens is configured as the second outer side of the cemented doublet.

In some embodiments, the positive focal power lens is a plano-convex lens, a plane of the positive focal power lens is configured as the first outer side of the cemented doublet, and a convex surface of the positive focal power lens is cemented with the negative focal power lens; the cemented doublet further comprising a second outer side opposite to the first outer side, a side of the negative focal power lens opposite to the positive focal power lens is configured as the second outer side of the cemented doublet.

In some embodiments, the difference between the refractive index of the negative focal power lens and the refractive index of the positive focal power lens is greater than or equal to <NUM>.

In some embodiments, the difference between the Abbe number of the positive focal power lens and the Abbe number of the negative focal power lens is greater than or equal to <NUM>.

In some embodiments, a second outer side of the cemented doublet is coated with an anti-reflection film.

In some embodiments, a flow channel is arranged inside the flow chamber; the first side and the second side are both parallel to the flow channel.

In some embodiments, the reflector is a plano-convex mirror, and a plane of the reflector is glued on the first side of the flow chamber.

In some embodiments, the surface of the reflector opposite to the flow chamber is aspheric.

In some embodiments, a detection apparatus, comprising: the light energy collecting system provided by the present disclosure; and a photoelectric detection module, configured to detect the light energy collected by the light energy collecting system and convert it into electrical signals.

The technical solutions of embodiments of the present disclosure have the following advantages: the detection apparatus provided by some embodiments of the present disclosure, wherein the light energy collecting system is arranged, the light energy collecting system can effectively correct the chromatic aberration to obtain a smaller focused spot, resulting high light energy collecting efficiency, which is beneficial to improve the efficiency of the photoelectric detection module to detect and photoelectrically convert the light energy collected by the light energy collecting system, thereby improving the detection and analysis efficiency of the detection apparatus; moreover, the reliability and stability of the light energy collecting system are high, and the structure is simple and the cost is low, which is beneficial to improve the reliability and stability of the detection apparatus, simplify the structure of the detection device and reduce the cost.

In some embodiments, the detection apparatus is a flow cytometer.

The accompanying drawings, which comprise a part of this specification, illustrate several embodiments and, together with the description, serve to explain the principles and features of the disclosed embodiments. In the drawings:.

It can be seen from the prior art that the current light energy collecting system cannot solve the problems of correcting chromatic aberration, system complexity or poor reliability, and high cost at the same time. Combining two light energy collecting systems, we analyze the reasons why the problems of correcting chromatic aberration, system complexity or poor reliability, and high cost cannot be solved at the same time.

<FIG> is a schematic diagram of a structure of a light energy collecting system.

Referring to <FIG>, the light energy collecting system shown in <FIG> comprises:.

In the light energy collecting system shown in <FIG>, only a single aspherical lens is used to correct part of the aberration. However, a single aspheric lens cannot effectively correct chromatic aberration, resulting in a limited effect on improving the concentration of the spot.

Moreover, the first lens <NUM> is a meniscus aspheric lens, and the meniscus aspheric lens is only glued to the flow cell <NUM> at the peripheral non-transparent part, but the effective aperture is not glued to the flow cell <NUM>, resulting in a small glued area and poor bonding strength, which makes that the first lens <NUM> easy to peel off from the flow cell <NUM> by an external force, or dust easy to enter into the glued surface which is difficult to remove when there are local small cracks on the glued surface, thereby further affect the convergence of light energy; furthermore, the meniscus aspheric lens is only glued with the flow cell <NUM> at the peripheral non-transparent part, to increase the transmittance of light energy, it is necessary to coat the two light-passing surfaces of the meniscus aspheric lens and the surface of the flow cell <NUM> opposite to the meniscus aspheric lens, thereby resulting in more surfaces need to be coated with anti-reflection films and easily leading to higher costs.

<FIG> is a schematic diagram of a structure of another light energy collecting system.

The light energy collecting system shown in <FIG> comprises: a prismatic glass test tube, comprising a flow channel <NUM>'; a reflector <NUM>', comprising a first side optically coupled to the prismatic glass test tube; corrector plate <NUM>', comprising a flat surface and an aspheric surface opposite to each other, the flat surface optically coupled to a second side of the prismatic glass test tube; and a color-compensating doublet lens <NUM>, inserted between the corrector plate <NUM>' and an image plane <NUM>', and the color compensating doublet lens <NUM> is spaced apart from the corrector plate <NUM>'.

In the light energy collecting system shown in <FIG>, the residual chromatic aberration introduced by the aspheric corrector plate <NUM>' is corrected by the color-compensating doublet lens <NUM>. However, the color compensation doublet lens <NUM> is set separately from the prismatic glass test tube, and an additional structure is required to fix and assemble the color compensation doublet lens <NUM>, which results in a relatively complicated process and structure as well as high requirements on the stability of the system, thereby leading to high cost and poor system reliability.

To solve the technical problem, some embodiments of the present disclosure provide a light energy collecting system, wherein a cemented doublet is arranged which comprises a negative focal power lens and a positive focal power lens cemented together, and the refractive index of the negative focal power lens is greater than that of the positive focal power lens, and the Abbe number of the negative focal power lens is smaller than the Abbe number of the positive focal power lens, thereby utilizing the dispersion properties of positive and negative focal power lenses with different refractive indices and Abbe numbers, the dispersion can be compensated for each other to achieve the purpose of correcting or eliminating chromatic aberration, which is beneficial to obtain a smaller focused spot and correspondingly improving the concentration of light energy.

Moreover, the first outer side of the doublet is planar such that the first outer side of the doublet can be fully glued to the second side of the flow chamber, which is beneficial to improve the bonding strength of the cemented doublet and the flow chamber, and correspondingly prevents the problem mis-alignment between the cemented doublet and the flow chamber during use, which affects the focusing effect, and prevents dust from entering the optical surface due to partial degumming and cracking on the edge which is difficult to remove, thereby improving the reliability of the light energy collecting system. In addition, the negative focal power lens and the positive focal power lens of the cemented doublet are cemented to each other, so that no additional structure is required for fixing and assembly, and there is no need to coat the first outer side of the cemented doublet and the second side of the flow chamber, which is beneficial to simplify the structure of light energy collecting system, reduce the cost, thereby improving the stability of the light energy collecting system.

To make the above objects, features, and advantages of the embodiments of the present disclosure more comprehensible, specific embodiments of the present disclosure will be described in detail below in conjunction with the accompanying drawings. Referring to <FIG>, shows a schematic structural diagram of an embodiment of the light energy collecting system of the present disclosure.

As shown in <FIG>, in an embodiment, a light energy collecting system comprising: a flow chamber <NUM>, comprising a first side <NUM> and a second side <NUM> opposite to each other; a reflector <NUM>, glued on the first side <NUM> of the flow chamber <NUM>; and a cemented doublet <NUM>, comprising a first outer side <NUM> which is planar and cemented to the second side <NUM> of the flow chamber <NUM>; the cemented doublet <NUM> comprising a negative focal power lens <NUM> and a positive focal power lens <NUM> cemented to each other, the refractive index of the negative focal power lens <NUM> is greater than the refractive index of the positive focal power lens <NUM>, and the Abbe number of the negative focal power lens <NUM> is smaller than the Abbe number of the positive focal power lens <NUM>.

The flow chamber <NUM> is configured to provide a flow channel <NUM> for the analytes so that the analytes (such as cells or particles, etc.) can be arranged in a row and sequentially advance in the center of the flow chamber <NUM>, and a light source can irradiate the analytes passing sequentially in the direction perpendicular to the flow direction of the analytes, and then scattered light is generated which can be collected by the light energy collecting system. Wherein, when the analytes are marked with a fluorescent dye, fluorescence can also be generated when the light source irradiates the analytes, and correspondingly, the light energy collecting system can also collect fluorescent signals.

Specifically, in an embodiment, there is a flow channel <NUM> inside the flow chamber <NUM>, the flow channel <NUM> is configured to provide a channel of flowing for the liquid flow or gas flow containing the analytes so that the light source can irradiate the analytes in a direction perpendicular to the flow channel <NUM>.

In an embodiment, the analytes can be cells or particles, etc..

In this embodiment, the flow channel <NUM> is disposed in the center of the flow chamber <NUM>.

In this embodiment, the flow chamber <NUM> comprises a first side <NUM> and a second side <NUM> opposite to each other, the first side <NUM> and the second side <NUM> are both parallel to the flow channel <NUM>, so that the light source can irradiate the analytes in a direction perpendicular to the flow channel <NUM> to generate scattered light, thereby the reflector <NUM> and the cemented doublet <NUM> correspondingly on the first side <NUM> and the second side <NUM> of the flow chamber <NUM> respectively can collect scattered light (including fluorescence).

In this embodiment, the first side <NUM> and the second side <NUM> of the flow chamber <NUM> are both planar, which is not only convenient for manufacturing, but also facilitates the bonding between the flow chamber <NUM> and the reflector <NUM> and the cemented doublet <NUM> respectively, further improving the bonding strength and reliability between flow chamber <NUM> and reflector <NUM> and cemented doublet <NUM>.

The material of the flow chamber <NUM> is a light-transmitting material. As an example, the material of the flow chamber <NUM> is glass, such as fused silica (F_silica). In other embodiments, based on actual design requirements, other suitable materials can also be used for the flow chamber.

The reflector <NUM> is configured to reflect and converge the scattered light generated by the analytes flowing in the flow chamber <NUM>, which facilitates the correction of aberration and chromatic aberration by the cemented doublet <NUM> to obtain a smaller focused spot.

In this embodiment, the reflector <NUM> is a plano-convex mirror, and a plane of the reflector <NUM> is glued on the first side <NUM> of the flow chamber <NUM>, thus, the plane of the reflector <NUM> can be glued to the flow chamber <NUM> on the entire surface, thereby increasing the glued area of the reflector <NUM> and the flow chamber <NUM>, improving the bonding strength and stability between the reflector <NUM> and the flow chamber <NUM>, and correspondingly improving the reliability of the light energy collecting system.

In this embodiment, the surface of the reflector <NUM> opposite to the flow chamber <NUM> is aspheric, compared with the spherical surface of the reflector on the side opposite to the flow chamber, the aspherical surface allows the reflector <NUM> to reflect and converge the scattered light generated on the analytes while reducing aberrations, thereby improving the thinness of the reflector <NUM> and reduce the volume of the light energy collection system accordingly.

More specifically, in an embodiment, the convex surface of the reflector <NUM> is aspheric.

Correspondingly, in this embodiment, the reflector <NUM> is relatively thin. In an embodiment, the thickness of the reflector <NUM> is <NUM>. In other embodiments, based on actual design requirements, the thickness of the reflector can also be other values.

In other embodiments, based on actual design requirements, the surface of the reflector on the side opposite to the flow chamber can also be a spherical surface, which is beneficial to save costs.

In this embodiment, the convex surface of the reflector <NUM> is coated with an internal reflection film, thereby improving the reflective effect of the reflector <NUM> on the scattered light generated on the analytes.

In this embodiment, the material of the reflector <NUM> is optical glass, such as D-k9 and the like.

The cemented doublet <NUM> is configured to transmit the light reflected by the reflector <NUM> to correct aberrations and chromatic aberrations, to obtain a smaller focused spot, thereby improving the concentration of light energy.

In this embodiment, the cemented doublet <NUM> comprises a negative focal power lens <NUM> and a positive focal power lens <NUM> cemented together, and the refractive index of the negative focal power lens <NUM> is greater than that of the positive focal power lens <NUM>, and the Abbe number of the negative focal power lens <NUM> is smaller than the Abbe number of the positive focal power lens <NUM>, thereby utilizing the dispersion properties of positive and negative focal power lenses with different refractive indices and Abbe numbers, the dispersion can be compensated for each other to achieve the purpose of correcting or eliminating chromatic aberration, which is beneficial to obtain a smaller focused spot and correspondingly improve the concentration of light energy.

Moreover, the first outer side <NUM> of the cemented doublet <NUM> is planar such that the first outer side <NUM> of the cemented doublet <NUM> can be fully glued to the second side <NUM> of the flow chamber <NUM>, which is beneficial to improve the bonding strength of the cemented doublet <NUM> and the flow chamber <NUM>, and correspondingly prevents the mis-alignment between the cemented doublet <NUM> and the flow chamber <NUM> during use, which affects the focusing effect, and prevents dust from entering the optical surface due to partial degumming and cracking on the edge which is difficult to remove, thereby improving the reliability of the light energy collecting system.

In addition, the negative focal power lens <NUM> and the positive focal power lens <NUM> of the cemented doublet <NUM> are cemented to each other, so that no additional structure is required for fixing and assembly, and there is no need to coat the first outer side <NUM> of the cemented doublet <NUM> and the second side <NUM> of the flow chamber <NUM>, which is beneficial to simplify the structure of light energy collecting system and reduce the cost, thereby improving the stability of the light energy collecting system.

In this embodiment, the cemented surface <NUM> of the negative focal power lens <NUM> and the positive focal power lens <NUM> is arranged opposite to the second side <NUM> of the flow chamber <NUM>, to allow the light reflected by the reflector <NUM> to pass through the negative focal power lens <NUM> and the positive focal power lens <NUM> sequentially, or pass through the positive focal power lens <NUM> and the negative focal power lens <NUM> sequentially, thus, the negative focal power lens <NUM> and the positive focal power lens <NUM> can cooperate to reduce chromatic aberration.

In this embodiment, the cemented doublet <NUM> further comprising a second outer side <NUM> opposite to the first outer side <NUM>, and the second outer side <NUM> is aspheric, compared with the solution of correcting the aberration with only one aspherical lens, some embodiments of the present disclosure adds an additional lens (that is, the lens of which the outer side is used as the first outer side) glued with the aspheric lens by setting the cemented doublet <NUM>, which is beneficial to further correct chromatic aberration, thereby helping obtain a smaller focused spot and correspondingly improving the concentration of light energy.

It should be noted that, in this embodiment, the optical axis of the cemented doublet <NUM> is basically coincident with the optical axis of the reflector <NUM>, so that the cemented doublet <NUM> can transmit the light reflected by the reflector <NUM>, and then the aberrations and chromatic aberrations can be corrected by the cemented doublet <NUM>.

In this embodiment, the second outer side <NUM> of the cemented doublet <NUM> is coated with an anti-reflection film, which is configured to reduce or eliminate the reflected light of the cemented doublet <NUM> on the second outer surface <NUM>, thereby increasing the amount of light transmitted and reducing or eliminating stray light.

Moreover, in this embodiment, since the first outer side <NUM> is planar and cemented to the second side <NUM> of the flow chamber <NUM>, and the negative focal power lens <NUM> and the positive focal power lens <NUM> are cemented to each other, there is no need to coat the cemented surface between the first outer side <NUM> and the flow chamber <NUM>, as well as the cemented surface between the negative focal power lens <NUM> and the positive focal power lens <NUM>, which is beneficial to simplify the process and save costs.

In this embodiment, the cemented surface <NUM> between the negative focal power lens <NUM> and the positive focal power lens <NUM> is spherical. The spherical lens cementing process is relatively mature, which is beneficial to improve the cementing reliability between the negative focal power lens <NUM> and the positive focal power lens <NUM>, and is also beneficial to save costs.

In other embodiments, based on actual design requirements, the cemented surface between the negative focal power lens and the positive focal power lens can also be an aspheric surface.

In this embodiment, the negative focal power lens <NUM> and the positive focal power lens <NUM> are cemented together by optical glue.

In this embodiment, the cemented doublet <NUM> comprises a negative focal power lens <NUM> and a positive focal power lens <NUM> cemented together, and the refractive index of the negative focal power lens <NUM> is greater than that of the positive focal power lens <NUM>, and the Abbe number of the negative focal power lens <NUM> is smaller than the Abbe number of the positive focal power lens <NUM>, the dispersion ability of the negative focal power lens <NUM> is different from that of the positive focal power lens <NUM>, therefore, the chromatic aberration can be compensated through the combination of two types of lenses with different focal powers, which have strong dispersion ability and weak dispersion ability, and the correction effect on chromatic aberration can be improved, thereby significantly reducing the focused spot and improving the concentration of light energy.

It should be noted that the difference between the refractive index of the negative focal power lens <NUM> and the refractive index of the positive focal power lens <NUM> should not be too small, otherwise it is easy to cause the correction effect of the cemented doublet <NUM> on chromatic aberration to be insignificant. For this reason, in this embodiment, the difference between the refractive index of the negative focal power lens <NUM> and the refractive index of the positive focal power lens <NUM> is greater than or equal to <NUM>.

It should also be noted that the difference between the Abbe number of the positive focal power lens <NUM> and the Abbe number of the negative focal power lens <NUM> should not be too small, otherwise the correction effect of the cemented doublet <NUM> on chromatic aberration is insignificant. In this embodiment, the difference between the Abbe number of the positive focal power lens <NUM> and the Abbe number of the negative focal power lens <NUM> is greater than or equal to <NUM>.

As an example, the material of the negative focal power lens <NUM> is crown glass, and the material of the positive focal power lens <NUM> is flint glass, thereby the dispersion ability of the negative focal power lens <NUM> is greater than the dispersion ability of the positive focal power lens <NUM>, and the refractive index of the negative focal power lens <NUM> is greater than the refractive index of the positive focal power lens <NUM>.

In other embodiments, the materials of the negative focal power lens and the positive focal power lens can also be other materials that satisfy the relationship between the above-mentioned refractive index and Abbe number.

As an example, the negative focal power lens <NUM> is a plano-concave lens, a plane of the negative focal power lens <NUM> is configured as the first outer side <NUM> of the cemented doublet <NUM>, and a concave surface of the negative focal power lens <NUM> is cemented with the positive focal power lens <NUM>.

That is to say, in this embodiment, the plane of the negative focal power lens <NUM> is cemented with the second side <NUM> of the flow chamber <NUM>, thus the entire plane of the negative focal power lens <NUM> is cemented on the second side <NUM> of the flow chamber <NUM> to increase the cemented area between the cemented doublet <NUM> and the flow chamber <NUM>, thereby improving the bonding strength and reliability between the cemented doublet <NUM> and the flow chamber <NUM>, and correspondingly improving the reliability of the light energy collecting system.

Correspondingly, in this embodiment, the concave surface of the negative focal power lens <NUM> is spherical, that is, the negative focal power lens <NUM> is a spherical lens, which is beneficial to reduce the cost.

As an example, the thickness of the negative focal power lens <NUM> is <NUM>, and the thickness of the negative focal power lens <NUM> is relatively thin, which is conducive to the thinning of the light energy collecting system.

The negative focal power lens <NUM> is made of optical glass. As an example, the material of the negative focal power lens <NUM> is H-ZLAF2A. In other embodiments, the material of the negative focal power lens can also be other suitable materials.

Correspondingly, in this embodiment, a side of the positive focal power lens <NUM> opposite to the negative focal power lens <NUM> is configured as the second outer side <NUM> of the cemented doublet <NUM>. That is to say, the side of the positive focal power lens <NUM> opposite to the negative focal power lens <NUM> is in contact with air and is an aspheric surface, which is conducive to the thinning of the positive focal power lens <NUM> and improves the correcting effect of positive focal power lens <NUM> on aberration.

Correspondingly, in this embodiment, the side of the positive focal power lens <NUM> cemented with the negative focal power lens <NUM> is a convex surface. Moreover, in this embodiment, the side of the positive focal power lens <NUM> cemented with the negative focal power lens <NUM> is a spherical convex surface, to achieve a close fit with the negative focal power lens <NUM>.

Correspondingly, in this embodiment, the surface of the positive focal power lens <NUM> opposite to the negative focal power lens <NUM> is coated with an anti-reflective film.

As an example, the thickness of the positive focal power lens <NUM> is <NUM>, and the positive focal power lens <NUM> is relatively thin, which is conducive to thinning the light energy collecting system.

The positive focal power lens <NUM> is made of optical glass. As an example, the material of the positive focal power lens <NUM> is H-K50. In other embodiments, the material of the positive focal power lens <NUM> can also be other suitable materials.

Referring to <FIG>, a schematic diagram of a focused light spot obtained by the light energy collecting system provided in this embodiment is shown. Wherein, <FIG> are focused spots of image plane (IMA) <NUM> and object plane (OBJ) - <NUM>, image plane <NUM> and object plane <NUM>, and image plane -<NUM> and object plane <NUM> respectively, at scale <NUM>.

As shown in <FIG>, the focused light spot obtained by the light energy collecting system provided by this embodiment is small, which improves the concentration of light energy.

Referring to <FIG>, a graph of axial chromatic aberration obtained by the light energy collecting system provided by this embodiment is shown. As shown in <FIG>, the abscissa is the spot size (unit: mm/millimeter), and the ordinate is the normalized radius of the lens (Normalized Pupil Coordinate). It can be seen from <FIG> that at each position of the ordinate, the range of the light spot of each wavelength is small, that is to say, the light energy collecting system provided by this embodiment can effectively reduce chromatic aberration and obtain a smaller light spot.

<FIG> is a structural schematic diagram of another embodiment of the light energy collecting system provided by the present disclosure. The similarities between this embodiment and the foregoing embodiments will not be repeated here, and the differences between this embodiment and the foregoing embodiments are:.

In this embodiment, the positive focal power lens <NUM> is a plano-convex lens, a plane of the positive focal power lens <NUM> is configured as the first outer side <NUM> of the cemented doublet <NUM>, and a convex surface of the positive focal power lens <NUM> is cemented with the negative focal power lens <NUM>.

That is to say, in this embodiment, the plane of the positive focal power lens <NUM> is cemented with the second side <NUM> of the flow chamber <NUM>, thus the entire plane of the positive focal power lens <NUM> is cemented on the second side <NUM> of the flow chamber <NUM> to increase the cemented area between the cemented doublet <NUM> and the flow chamber <NUM>, thereby improving the bonding strength and reliability between the cemented doublet <NUM> and the flow chamber <NUM>, and correspondingly improving the reliability of the light energy collecting system.

Correspondingly, in this embodiment, the convex surface of the positive focal power lens <NUM> is spherical, that is, the positive focal power lens <NUM> is a spherical lens, which is beneficial to reduce the cost.

In this embodiment, the thickness of the positive focal power lens <NUM> is <NUM>, and the positive focal power lens <NUM> is relatively thin, which is conducive to thinning the light energy collecting system. In other embodiments, based on actual design requirements, the positive focal power lens may also have other thicknesses.

Correspondingly, in this embodiment, a side of the negative focal power lens <NUM> opposite to the positive focal power lens <NUM> is configured as the second outer side <NUM> of the cemented doublet <NUM>. That is to say, the side of the negative focal power lens <NUM> opposite to the positive focal power lens <NUM> is in contact with air and is an aspheric surface, which is conducive to the thinning of the negative focal power lens <NUM> and improves the correcting effect of negative focal power lens <NUM> on aberration and chromatic aberration.

Correspondingly, in this embodiment, the side of the negative focal power lens <NUM> cemented to the positive focal power lens <NUM> is a concave surface. Moreover, in this embodiment, the side of the negative focal power lens <NUM> cemented to the positive focal power lens <NUM> is a spherical concave surface, to realize a close fit with the positive focal power lens <NUM>.

Correspondingly, in this embodiment, the surface of the negative focal power lens <NUM> opposite to the positive focal power lens <NUM> is coated with an anti-reflective film.

As an example, the thickness of the negative focal power lens <NUM> is <NUM>, and the negative focal power lens <NUM> is relatively thin, which is conducive to thinning the light energy collecting system. In other embodiments, the negative focal power lens can also have other thicknesses.

In this embodiment, in the cemented doublet <NUM>, the positional relationship and collocation mode between the negative focal power lens <NUM> and the positive focal power lens <NUM> are different from those in the foregoing embodiments, thereby providing more options for the cemented doublet <NUM>, correspondingly improving the structural freedom and flexibility of the light energy collecting system.

Referring to <FIG>, a schematic diagram of a focused light spot obtained by the light energy collecting system provided by this embodiment. Wherein, <FIG> are focused spots of the image plane (IMA) <NUM> and object plane (OBJ) - <NUM>, image plane <NUM> and object plane <NUM>, and image plane -<NUM> and object plane <NUM> respectively, at scale <NUM>.

Correspondingly, the embodiment of the present disclosure also provides a detection apparatus. <FIG> is a functional block diagram of an embodiment of the detection apparatus provided by the present disclosure.

As shown in <FIG>, in this embodiment, the detection apparatus, comprising: the light energy collecting system <NUM> provided by the foregoing embodiments; and a photoelectric detection module <NUM>, configured to detect the light energy collected by the light energy collecting system <NUM> and convert it into electrical signals.

The detection apparatus provided by the embodiments of the present disclosure, wherein the light energy collecting system <NUM> is arranged, the light energy collecting system <NUM> can effectively correct the chromatic aberration to obtain a smaller focused spot, resulting in high light energy collecting efficiency, which is beneficial to improve the efficiency of the photoelectric detection module <NUM> to detect and photoelectrically convert the light energy collected by the light energy collecting system <NUM>, thereby improving the detection and analysis efficiency of the detection apparatus; moreover, the reliability and stability of the light energy collecting system <NUM> are high, and the structure is simple and the cost is low, which is beneficial to improve the reliability and stability of the detection apparatus, simplify the structure of the detection device and reduce the cost.

As an example, the detection apparatus is a flow cytometer, which is configured to detect and analyze the characteristics of the cells. In other embodiments, the detection apparatus can also be other types of detection apparatuses, such as air particle detectors, etc..

The light energy collecting system <NUM> is configured for collecting and converging the light generated by the analytes, so that the light can be detected by the photoelectric detection module <NUM>.

For the detailed description of the light energy collecting system <NUM>, reference may be made to the corresponding descriptions in the foregoing embodiments, and no further details are given here.

The photoelectric detection module <NUM> is configured to detect the light energy collected by the light energy collecting system <NUM> and convert it into electrical signals, thereby realizing photoelectric conversion. The photoelectric detection module <NUM> includes a photoelectric conversion device, such as a photomultiplier tube (Photo Multiplier Tube, PMT) and the like.

In a specific implementation, the detection apparatus may also include other structures, such as a signal processing module, which is configured to analyze and process the electrical signals to analyze the characteristics of the analytes. The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present disclosure. A variety of modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the scope of the present disclosure. Accordingly, the present disclosure will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claim 1:
A light energy collecting system (<NUM>), comprising:
a flow chamber (<NUM>, <NUM>), comprising a first side (<NUM>, <NUM>) and a second side (<NUM>, <NUM>) opposite to each other;
a reflector (<NUM>), glued on the first side (<NUM>) of the flow chamber (<NUM>, <NUM>); and
a cemented doublet (<NUM>, <NUM>), comprising a first outer side (<NUM>) which is planar and cemented to the second side (<NUM>, <NUM>) of the flow chamber (<NUM>, <NUM>); the cemented doublet (<NUM>, <NUM>) comprising a negative focal power lens (<NUM>, <NUM>) and a positive focal power lens (<NUM>, <NUM>) cemented to each other, the refractive index of the negative focal power lens (<NUM>, <NUM>) is greater than the refractive index of the positive focal power lens (<NUM>, <NUM>), and the Abbe number of the negative focal power lens (<NUM>, <NUM>) is smaller than the Abbe number of the positive focal power lens (<NUM>, <NUM>).