Patent Description:
Crohn's Disease is a chronic, relapsing inflammatory disease of the gastrointestinal tract, characterized by segmental transmural inflammation and granulomatous changes. Typical presentations include the discontinuous involvement of various portions of the gastrointestinal tract and the development of complications including strictures, abscesses or fistulas. Because its cause is unknown, medical management of Crohn's disease is largely empirical and is designed to reduce inflammation. Medical therapy includes corticosteroids, antibiotics, immunosuppressant drugs, and anti-TNFα agents. Due to the therapeutic failures and serious side effects of present therapies, alternatives are needed.

An important role in the pathogenesis of IBD is played by TGF-β1, a multifunctional cytokine capable of regulating the growth, differentiation, and function of immune and non-immune cells. A diminished ability to mount an efficient counter-regulatory TGF-β1 response to inflammatory stimuli is believed to be relevant in the pathogenesis of disease such as IBD. TGF-β1 acts as a potent negative regulator of mucosal inflammation and that the inhibition of its activity results in the development of colitis which shows immunomorphological similarities with Crohn's disease or ulcerative colitis.

In the inflamed intestine of patients with IBD there is marked over expression of Smad7 (a protein that serves as substrates for TGF-β1 receptors) and a reduction of Smad <NUM> phosphorylation, a crucial step in the TGF-β1 mediated signal transduction. Thus, in IBD, high levels of Smad7 may lead to a defective TGF-β1 signaling resulting in an over-expression of pro-inflammatory molecules genes and TGF-β1 does not exert its anti-inflammatory role.

Antisense oligodeoxynucleotide drugs are short chains of DNA nucleotides that inhibit protein translation by specifically binding to a small segment of messenger RNA (mRNA) responsible for driving the production of disease-causing proteins. The sequence of an antisense drug is designed to be complementary to its mRNA target such that, upon hybridization, the resulting double-stranded segment is recognized by the cell as abnormal and is destroyed, thereby preventing translation of the message into the protein product.

Antisense therapeutics, however, are typically administered parenterally which can lead to adverse reactions due to systemic effects. Such administration may also be unable to localize at the site of needed treatment. Therefore, there is a need for a topical-like application of antisense treatments for the treatment of IBD and related diseases using tablet based formulations.

<CIT> relates to antisense oligonucleotidic sequences (ODN) against Smad7 suitably modified, and their uses in medical field as therapeutic biological agents, in particular in the treatment of chronic inflammatory bowel disease, such as Crohn's disease and ulcerative colitis. <CIT> relates to modified release pharmaceutical formulations and methods for enhanced mucosal drug absorption. The formulation comprises initial population(s) of particles comprising both drug and penetration enhancer which are released at a first location in the gastrointestinal tract, and a subsequent population or populations of particles comprising a penetration enhancer(s) having a delayed release due to a polymeric coating or matrix. This penetration enhancer is released at an additional location(s) in the intestine downstream from the first location and enhances absorption of the drug when it reaches the additional location(s).

This disclosure is directed, at least in part, to pharmaceutical formulations for oral administration of antisense oligonucleotides, such as antisense oligonucleotides against SMAD7.

In an embodiment, a pharmaceutical tablet formulation for oral administration of an antisense oligonucleotide is provided that comprises an intra-granular phase, wherein the intra-granular phase includes an antisense oligonucleotide represented by SEQ ID NO <NUM>, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable filler, and which includes an extra-granular phase comprising a disintegrant. Contemplated oligonucleotides include those represented by SEQ ID NO <NUM>, wherein at least one, or in certain embodiments, all, the internucleotide linkages are O, O- linked phosphorothioates.

The present disclosure provides for a tablet that includes a disclosed antisense oligonucleotide, and comprises an enteric coating. Such a tablet may, for example, include a filler, a disintegrant, and/or a lubricant. For example, provided herein is an oral dose form, such as a tablet, that comprises about <NUM> to about <NUM> of an antisense oligonucleotide, e.g. <NUM> of an oligonucleotide represented by SEQ ID NO <NUM> or a pharmaceutically acceptable salt thereof.

In an embodiment, provided herein is a tablet for oral use comprising: about <NUM>% to about <NUM>% by weight of an antisense oligonucleotide represented by SEQ ID NO <NUM> or a pharmaceutically acceptable salt thereof; about <NUM>% to about <NUM>% by weight mannitol; and about <NUM>% to about <NUM>% by weight microcrystalline cellulose.

For example, the disclosure provides a pharmaceutically acceptable tablet for oral use comprising an intra-granular phase and extra-granular phase, wherein for example, the intra-granular phase comprises about <NUM>% to about <NUM>%, by weight (for example about <NUM>% by weight) of an antisense oligonucleotide represented by SEQ ID NO <NUM> or a pharmaceutically acceptable salt thereof, about <NUM>% by weight mannitol, about <NUM>% by weight microcrystalline cellulose, about <NUM>% by weight hydropropylmethyl cellulose, and about <NUM>% by weight sodium starch glycolate, and for example, the extra-granular phase comprises about <NUM>% by weight microcrystalline cellulose, about <NUM>% by weight sodium starch glycolate, and about <NUM>% by weight magnesium stearate, where the tablet may further comprise an enteric coating.

Also provided herein are methods for treating Crohn's disease, ulcerative colitis, and chronic inflammatory bowel disease comprising administering to the patient in need thereof a tablet, oral dose or pharmaceutical formulation disclosed herein. For example, upon orally administering the pharmaceutical formulation, tablet or oral dosage form to a patient, the pharmaceutical formulation, tablet or oral dosage form may be substantially delivered to the terminal ileum and/or right colon of the patient.

The present disclosure is generally directed to pharmaceutical compositions that include an antisense oligonucleotide, such as that depicted in <FIG>. Contemplated compositions include oligonucleotides that act against Smad7, and may be administered orally. Disclosed compositions may, when administered orally, deliver an effective amount of an antisense oligonucleotide to the intestinal system of a patient, e.g. deliver an effective amount of an antisense oligonucleotide to the terminal ileum and/or right colon of a patient.

Contemplated antisense oligonucleotides include those comprising SEQ ID NO: <NUM> GTC* GCC CCT TCT CCC C*GC AGC, where C* represents <NUM>-methyl-<NUM>'-deoxycytidine. In some embodiments, at least one of the internucleotide linkages of a contemplated antisense oligonucleotide is a O,O-linked phosphorothioate, for example, each of the <NUM> internucleotide linkages of SEQ ID NO: <NUM> may be a O,O-linked phosphorothioate. In some embodiments, contemplated compositions disclosed herein may include a pharmaceutically acceptable salt of the antisense oligonucleotide of SEQ ID NO : <NUM>, that optionally may include <NUM> to <NUM> O,O-linked phosphorothioate internucleotide linkages. Contemplated salts of oligonucleotides include those that are fully neutralized. Oligonucleotides may include naturally occurring nucleobases, sugars, and covalent internucleoside (backbone) linkages as well as non-naturally occurring portions. An exemplary antisense oligonucleotide, referred herein as AS1, is shown in <FIG>.

In some embodiments, contemplated herein are compositions suitable for oral delivery of an antisense oligonucleotide e.g., tablets, that include an enteric coating, e.g., a gastro-resistant coating, such that the compositions may deliver the antisense compound to e.g. the terminal ileum and right colon of a patient. For example, such administration may result in a topical effect, substantially topically applying the antisense compound directly to an affected portion of the intestine of a patient. Such administration, may, in some embodiments, substantially avoid unwanted systemic absorption of the antisense compound.

For example, a tablet for oral administration is provided that comprises granules (e.g., is at least partially formed from granules) that include a disclosed antisense compound, e.g., AS1, and pharmaceutically acceptable excipients. Such a tablet may be coated with an enteric coating. Contemplated tablets may include pharmaceutically acceptable excipients such as fillers, binders, disintegrants, and/or lubricants, as well as coloring agents, release agents, coating agents, sweetening, flavoring such as wintergreen, orange, xylitol, sorbitol, fructose, and maltodextrin, and perfuming agents, preservatives and/or antioxidants.

In some embodiments, contemplated pharmaceutical formulations include an intra-granular phase that includes a contemplated antisense compound, e.g. that depicted in SEQ ID NO. <NUM>, or a pharmaceutically acceptable salt, e.g. AS1, and a pharmaceutically acceptable filler. For example, AS1 and a filler may be blended together, with optionally other excipients, and formed into granules. In some embodiments, the intragranular phase may be formed using wet granulation, e.g. a liquid (e.g., water) is added to the blended antisense compound and filler, and then combination is dried, milled and/or sieved to produce granules. One of skill in the art would understand that other processes may be used to achieve an intragranular phase.

In some embodiments, contemplated formulations include an extra-granular phase, which may include one or more pharmaceutically acceptable excipients, and which may be blended with the intragranular phase to form a disclosed formulation.

A disclosed formulation may include a intragranular phase that includes a filler. Exemplary fillers include, but are not limited to, cellulose, gelatin, calcium phosphate, lactose, sucrose, glucose, mannitol, sorbitol, microcrystalline cellulose, pectin, polyacrylates, dextrose, cellulose acetate, hydroxypropylmethyl cellulose, partially pregelatinized starch, calcium carbonate, and others including combinations thereof.

In some embodiments, a disclosed formulation may include a intragranular phase and/or a extragranular phase that includes a binder, which may generally function to hold the ingredients of the pharmaceutical formulation together. Exemplary binders include invention may be, but are not limited to, the following: starches, sugars, cellulose or modified cellulose such as hydroxypropyl cellulose, lactose, pregelatinized maize starch, polyvinyl pyrrolidone, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, low substituted hydroxypropyl cellulose, sodium carboxymethyl cellulose, methyl cellulose, ethyl cellulose, sugar alcohols and others including combinations thereof.

Contemplated formulations, e.g., that include an intragranular phase and/or an extragranular phase, may include a disintegrant such as but are not limited to, starch, cellulose, crosslinked polyvinyl pyrrolidone, sodium starch glycolate, sodium carboxymethyl cellulose, alginates, corn starch, crosmellose sodium, crosslinked carboxymethyl cellulose, low substituted hydroxypropyl cellulose, acacia, and others including combinations thereof. For example, an intragranular phase and/or an extragranular phase may include a disintegrant.

In some embodiments, a contemplated formulation includes an intra-granular phase comprising a disclosed antisense compound and excipients chosen from: mannitol, microcrystalline cellulose, hydroxypropylmethyl cellulose, and sodium starch glycolate or combinations thereof, and an extra-granular phase comprising one or more of: microcrystalline cellulose, sodium starch glycolate, and magnesium stearate or mixtures thereof.

In some embodiments, a contemplated formulation may include a lubricant, e.g. an extra-granular phase may contain a lubricant. Lubricants include but are not limited to talc, silica, fats, stearin, magnesium stearate, calcium phosphate, silicone dioxide, calcium silicate, calcium phosphate, colloidal silicon dioxide, metallic stearates, hydrogenated vegetable oil, corn starch, sodium benzoate, polyethylene glycols, sodium acetate, calcium stearate, sodium lauryl sulfate, sodium chloride, magnesium lauryl sulfate, talc, and stearic acid.

In some embodiments, the pharmaceutical formulation comprises an enteric coating. Generally, enteric coatings create a barrier for the oral medication that controls the location at which the drug is absorbed along the digestive track. Enteric coatings may include a polymer that disintegrates a different rates according to pH. Enteric coatings may include for example, cellulose acetate phthalate, methyl acrylate-methacrylic acid copolymers, cellulose acetate succinate, hydroxylpropylmethyl cellulose phthalate, methyl methacrylate-methacrylic acid copolymers, ethylacrylate-methacrylic acid copolymers, methacrylic acid copolymer type C, polyvinyl acetate-phthalate, and cellulose acetate phthalate.

Exemplary enteric coatings include Opadry® AMB, Acryl-EZE®, Eudragit® grades. In some embodiments, an enteric coating may comprise about <NUM>% to about <NUM>%, about <NUM>% to about <NUM>%, <NUM> to about <NUM>%, about <NUM>% to about <NUM>%, about <NUM>% to about <NUM>%, or about <NUM> to about <NUM>%, of a contemplated tablet by weight. For example, enteric coatings may include an ethylacrylate-methacrylic acid copolymer.

For example, a tablet is provided that comprises or consists essentially of about <NUM>% to about <NUM>%, e.g. about <NUM>% to about <NUM>%, or about <NUM>% to about <NUM>%, by weight of an antisense oligonucleotide or a pharmaceutically acceptable salt thereof (e.g. AS1). Such a tablet may include for example, about <NUM>% to about <NUM>% by weight of mannitol, e.g. about <NUM>% to about <NUM>% by weight mannitol, e.g. about <NUM>% by weight mannitol; and/or about <NUM>% to about <NUM>% by weight of microcrystalline cellulose, or about <NUM>% to about <NUM>% by weight of microcrystalline cellulose. For example, a disclosed tablet may comprise a intra granular phase that includes about <NUM>% to about <NUM>%, e.g. about <NUM>% to about <NUM>% by weight, or alternatively, about <NUM> to about <NUM>% by weight AS1, about <NUM>% to about <NUM>%, or alternatively, about <NUM>% to about <NUM>% by weight mannitol, about <NUM>% to about <NUM>% microcrystalline cellulose, about <NUM>% to about <NUM>%, or about <NUM>% to about <NUM>% hydroxypropylmethylcellulose, and about <NUM>% to about <NUM>%, e.g. about <NUM>% to about <NUM>% sodium starch glycolate by weight.

Exemplary formulations include dosage forms that include or consist essentially of about <NUM> to about <NUM> of AS1, for example, tablets that include about <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM> of AS1 are contemplated herein.

In an exemplary embodiment, a pharmaceutically acceptable tablet for oral administration is provided that includes a intra-granular phase that may comprise about <NUM>% by weight AS1 (or salt thereof), about <NUM>% by weight mannitol, about <NUM>% by weight microcrystalline cellulose, about <NUM>% by weight hydropropylmethylcellulose, and about <NUM>% by weight sodium starch glycolate; and an extra-granular phase that may comprise about <NUM>% by weight microcrystalline cellulose, about <NUM>% by weight sodium starch glycolate, and about <NUM>% by weight magnesium stearate. The tablet may also include an enteric coating.

In another exemplary embodiment, a pharmaceutically acceptable tablet for oral administration is provided that includes or consists essentially of: a intra-granular phase that may comprise or consist essentially of about <NUM>% to about <NUM>%, e.g., about <NUM>% by weight AS1 (e.g. wherein the internucleotide linkages are each O,O-linked phophorothioates, and/or salt thereof, e.g. a sodium salt), about <NUM>% by weight mannitol, about <NUM>% by weight microcrystalline cellulose, about <NUM>% by weight hydropropylmethylcellulose, and about <NUM>. % by weight sodium starch glycolate; and an extra-granular phase that may comprise about <NUM>% by weight microcrystalline cellulose, about <NUM>% by weight sodium starch glycolate, and about <NUM>% by weight magnesium stearate.

Disclosed tablets may also include an enteric coating, e.g., a disclosed tablet may include about <NUM>%, about <NUM>%, <NUM>%, <NUM>% by weight of an enteric coating, e.g. AcyrlEZE®.

The rate at which point the coating dissolves and the active ingredient is released is its dissolution rate. In an embodiment, a contemplated tablet may have a dissolution profile, e.g. when tested in a USP/EP Type <NUM> apparatus (paddle) at <NUM> rpm and <NUM> in a phosphate buffer with a pH of <NUM>, of about <NUM>% to about <NUM>% of the oligonucleotide releasing after about <NUM> minutes to about <NUM> minutes, for example after <NUM> minutes. In another embodiment, a contemplated tablet may have a dissolution profile, e.g. when tested in a USP/EP Type <NUM> apparatus (paddle) at <NUM> rpm and <NUM> in diluted HCl with a pH of <NUM>, where substantially none of the oligonucleotide is released after <NUM> minutes. A contemplated tablet, in another embodiment, may have a dissolution profile, e.g. when tested in USP/EP Type <NUM> apparatus (paddle) at <NUM> rpm and <NUM> in a phosphate buffer with a pH of <NUM>, of about <NUM>% to about <NUM>%, or not more than about <NUM>%, of the oligonucleotide releasing after <NUM> minutes.

Disclosed formulations, e.g. tablets, in some embodiments, when orally administered to the patient may result in minimal plasma concentration of the oligonucleotide in the patient. In another embodiment, disclosed formulations, when orally administered to a patient, topically deliver to the terminal ileum and/or right colon of a patient, e.g. to an affected or diseased intestinal site of a patient.

Also provided herein are methods of treating Crohn's disease, ulcerative colitis, and/or chronic inflammatory bowel disease in a patient in need thereof administering a disclosed formulation.

The examples that follow are intended in no way to limit the scope of this invention but are provided to illustrate the methods of the present invention. Many other embodiments of this invention will be apparent to one skilled in the art.

Wet granules were prepared by dispensing each intra-granular component into an appropriate container. All of the intra-granular materials were screened through a <NUM> sieve and blended in a food processor bowl for around <NUM> minutes. Water granulating fluid was added slowly using a syringe. The wet mass was passed through <NUM> hand screen and dried in an oven at <NUM> for up to <NUM> minutes. After drying, the granules were screened through <NUM> hand screen. A mortar and pestle was employed to reduce the size of the coarse granules. The granules were blended with the extra-granular excipients except magnesium stearate using a Turbula blender for <NUM> minutes at 42rpm. Magnesium stearate was added to the blend and further mixed for <NUM> minutes at 42rpm. Formulations were compressed on a Manesty F3 single punch compression machine. An overall manufacturing process flow diagram for the AS1 tablets may be seen in <FIG>.

The mannitol based <NUM> dose strength formulation made at a <NUM> batch size had a moisture content of the dry mix of <NUM>% and a moisture content of the dried granules of <NUM>%. The mannitol based <NUM> dose strength formulation had <NUM> of water added, a granulation time of <NUM> minutes, and a drying time of <NUM> minutes.

The mannitol based <NUM> dose strength made at a <NUM> batch size had a moisture content of <NUM>% for the dried granules, <NUM> of water was added, <NUM> minutes of granulation time, and had a drying time of <NUM> minutes.

The compression IPC results for the mannitol based <NUM> dose strength formulations are as follows: average weight of <NUM>, a hardness of <NUM>. 0Kp, a thickness of <NUM>, a friability of <NUM>%, and a disintegration of <NUM> minutes.

Table 1A includes tablet compositions for three dose strengths: <NUM>, <NUM>, and <NUM>. Tablet weight was <NUM> for all formulations.

Formulations were assessed for particle size distribution, density, Carr's index and angle of repose. Particle size analysis indicated that particles are bigger in size (<NUM>) for <NUM> dose strength formulation, whereas <NUM> and <NUM> dose strength formulations exhibited usual particle size distribution. <FIG> depicts the particle size distribution for the <NUM> dose strength with the composition indicated in Table <NUM>. <FIG> depicts the particle size distribution of the <NUM> dose strength with the composition indicated in Table <NUM>. Finally, <FIG> depicts the particle size distribution for the <NUM> dose strength with the composition as indicated in Table <NUM>. Table <NUM> presents the powder characterization results for the three dose strengths.

A <NUM>% coating solution was prepared for Acryl-EZE® coatings. The required quantities of water and Acryl-EZE® were dispensed in appropriate containers. While mixing, Acryl-EZE® was added slowly to the vortex. The dispersion was stirred for 45mins and passed through a <NUM> sieve. The spraying was continued until a weight gain of <NUM>% or <NUM>% was obtained.

A batch formula for <NUM> tables of AS1 is depicted below:.

An AS1 tablet at <NUM> dose strength was manufactured generally following the procedure in Example <NUM>, as shown in Table <NUM>. The tablet weight for all the formulations was <NUM>.

This analytical test procedure describes a dissolution analysis of AS <NUM> enteric coated tablets by HPLC. Dissolution is preformed following the Ph. Procedure for delayed release solid dosage forms, using Method A. Apparatus used was Ph. /USP apparatus <NUM> (paddle).

The dissolution conditions for the HPLC are as follows: the media consists of pH <NUM> HCl (<NUM> mins), pH <NUM> (<NUM> mins), pH <NUM> (<NUM> mins); a temperature of <NUM>; a rate of <NUM> rpm; sample to recycle <NUM>, sample size <NUM>; sample times <NUM> in pH <NUM> HCl, <NUM>, <NUM> pH <NUM>, <NUM>, <NUM>, <NUM>, <NUM> pH <NUM>; and a <NUM> Disteck in-line filter.

Media was adjusted in each vessel during the dissolution at each state as follows: the initial volume <NUM> pH <NUM> HCl; at <NUM> mins, <NUM> of <NUM> Na<NUM>PO<NUM> and <NUM> <NUM> pH <NUM> Na<NUM>HPO<NUM> was added followed by the adjustment of the pH to pH <NUM>±<NUM> with <NUM> NaOH; at <NUM> mins, followed by the adjustment of the pH to <NUM>±<NUM> with <NUM> NaOH.

The chromatographic conditions are as follows: a Dionex HPLC analytical column, DNAPac-<NUM>, <NUM> x <NUM>; a flow rate of <NUM>/min; a column temperature of <NUM>; detection of UV at <NUM>; an injection volume of <NUM>µl; a needle wash of water; a mobile phase of A) <NUM>% v/v acetonitrile in <NUM> Tris (pH <NUM>) and B) <NUM>% v/v acetonitrile in <NUM> Tris and <NUM> LiCl (pH <NUM>); an HPLC run time of <NUM> mins; and an elution period of AS1 at approximately <NUM> minutes. The gradient is presented in Table <NUM>.

Preparation of <NUM> Tablets working standard solutions: <NUM> of AS1 reference standard was placed into <NUM> volumetric flask and dissolved with water to make <NUM> solution. <NUM> of the solution was diluted with water to make a <NUM> solution yielding a final concentration of AS1 of <NUM>/ml.

Preparation of <NUM> tablets working standard solutions: <NUM> of AS1 is placed in a <NUM> volumetric flask and dissolved with water to <NUM> volume yielding a final concentration of AS1 of <NUM>/ml.

Table <NUM> presents the dissolution results for the <NUM>, <NUM>, and <NUM> batches, with a <NUM>% weight gain Acryl-EZE® coating. <FIG> is a graph of the dissolution profiles for tablets with three dose sizes of AS1 formulations in three different mediums.

Tablets with <NUM>% coating of Acryl-EZE® are made using tablets as described above in Batch <NUM>, and the final coating is conducted until the weight gain of the tablets into the coating pan is <NUM>% of the starting value. <FIG> depicts the dissolution profile.

The objectives of the study were to identify the potential effects of AS1 on cardiovascular, respiratory, and central nervous systems of conscious, unrestrained, cynomolgus monkeys when administered a single dose by oral administration or intravenous administration. The intravenous administration in this study was included to investigate potential effects associated with systemic exposure. The intravenous dose formulation was administered as bulk powder and for oral administration as enteric coated tablets contained within gelatin capsules. Four experimentally non-naive cynomolgus male monkeys, <NUM> to <NUM> years of age, and weighing <NUM> to <NUM> were assigned to a single group.

All dose levels represent the quantity of AS1, corrected for purity. The control oral doses was two capsules containing placebo tablets. Approximate dose level, achieved by administration of two capsules, each containing a tablet with <NUM> AS1 per tablet (corrected for purity), and based on body weights that ranged from <NUM> to <NUM> on Day <NUM>. Approximate dose level, achieved by administration of two capsules, each containing tablet with <NUM> AS1 per tablet (corrected for purity), and based on body weights that ranged from <NUM> to <NUM> on Day <NUM>.

Blood samples for toxicokinetic analysis were collected prestudy and <NUM> and <NUM> hours post dose for oral doses and <NUM> minutes, <NUM> and <NUM> hours post dose for intravenous doses. The samples were processed to plasma under refrigerated conditions and the plasma was stored at -<NUM> until analyzed. The samples were analyzed using the AS1 specific hybridization assay.

On Day <NUM>, all animals received the oral control article dose, followed by doses of AS1 on Days <NUM> and <NUM> via oral gavage. For the oral doses, the placebo and AS1 containing tablets were contained within appropriate size gelation capsules to facilitate administration via the oral route. On Day <NUM>, all animals received the intravenous control dose (phosphate-buffered saline) by slow push injection, followed by intravenous doses of AS1 on Days <NUM> and <NUM>.

Cardiovascular data and body temperature data were recorded via telemetry at frequent intervals prior to and for <NUM> hours following each dose administration. Respiratory function was assessed by measurement of blood gas parameters in arterial samples collected prior to dosing and <NUM>, <NUM>, and <NUM> hours following each dose, as well as by determining respiration rate (visually) at those same time points. Neurologic function was assessed by performing a comprehensive neurologic examination of all animals prior to the study within approximately <NUM> hours after the end of the telemetry recording period following the last oral dose of test article and again within approximately <NUM> hours after the end of the telemetry recording period following the last intravenous dose. Animals were also observed for clinical signs (mortality/morbidity, cage side observations, food consumption, and body weight).

Despite a very low LLOQ for the assay (<NUM> ng/ml), AS1 was not detected in any plasma samples following oral doses up to nearly <NUM>/kg. In contrast, mean maximum plasma concentrations following IV injections were <NUM>,<NUM> and <NUM>,<NUM> ng/ml for the <NUM> and <NUM>/kg doses, respectively. Clearance of AS1 from the plasma following IC dosing exhibited kinetics similar to what has been reported for structurally related oligonucleotides (i.e., an approximate <NUM>-hour half-life).

The purpose of this study was to evaluate the potential toxicity and toxicokinetics of AS1 when administered once daily orally to mice for <NUM> days followed by a <NUM> day recovery period. The AS1 formulation was prepared as a formulation containing a gastro-protective coating in the form of small coated beads AS1 was layered onto inert beads which were then coated with Eudragit® S <NUM> to mimic the oral formulations suitable for human use. Three groups administered different dose levels were used (<NUM>/kg/day (low); <NUM>/kg/day; <NUM>/kg/day).

There were no gender-related differences in plasma or tissue levels. Despite a very low LLOQ for the assay, AS1 was detected in only two plasma samples from very low dose (<NUM>/kg/day) animals collected on Day <NUM> and not in any samples collected on Day <NUM>. At the higher dose levels of <NUM> and <NUM>/kg/day, AS1 was quantifiable in most plasma samples, and the levels were generally dose related. However, even at the highest dose level, the plasma levels did not exceed <NUM> ng/ml in any animal (in samples collected at various times between <NUM> and <NUM> hours post-dose). AS1 concentrations were very high in gastrointestinal (GI) tissues, with mean maximal concentrations at the highest dose level (following the first dose) of approximately <NUM>, <NUM>, <NUM>, <NUM>, <NUM> and <NUM>µg/gram of tissue for large intestine, small intestine, forestomach, glandular stomach, esophagus and rectum, respectively.

Extensive clearance from the GI tract tissues was evident by <NUM> hours after the first dose. There was no apparent accumulation of AS1 with daily dosing.

Maximal mean concentrations in the two major organs of systemic uptake, kidney and liver, following the first dose of <NUM>/kg were only <NUM> and <NUM>µg/gram, which is over <NUM> times lower than the range of concentrations measured in GI tissues.

Following the lowest dose of <NUM>/kg, the highest kidney and liver mean concentrations were only <NUM> and <NUM>µg/gram. There was no evidence for accumulation of AS1 in internal organs over the <NUM> day dosing period.

Systemic exposure to AS1 in mice was very low following oral administration of high doses (up to <NUM>/kg/day) of AS1, delivered in gastro-protected formulation and there was no accumulation in GI tissues or internal tissues when administered daily for <NUM> consecutive days.

To examine the therapeutic effect of AS1 on the course of the intestinal inflammation in the TNBS-induced colitis model, mice were treated with a single dose of AS1 or Smad7 sense oligonucleotide one day following intra-rectal administration of TNBS. Single doses of <NUM> or <NUM>µg/mouse ameliorated weight loss and markedly reduced the severity histological manifestations of colitis.

Claim 1:
A pharmaceutical tablet formulation for oral administration of an antisense oligonucleotide comprising:
an intra-granular phase comprising an antisense oligonucleotide comprising the nucleotide sequence of SEQ ID NO: <NUM> or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable filler;
an extra-granular phase comprising a disintegrant; and
an enteric coating comprising an ethylacrylate-methacrylic acid copolymer,
wherein the pharmaceutical tablet formulation, upon oral administration to a patient, delivers the antisense oligonucleotide substantially to the terminal ileum and/or right colon of the patient.