Patent Description:
<CIT>, as shown in <FIG>, discloses a microscope apparatus <NUM> including a stage <NUM> for placing a sample, a camera <NUM> for imaging a sample placed on the stage <NUM>, a light source <NUM> for irradiating transmission light for bright field observation toward the stage <NUM>, and a light source <NUM> for irradiating light for fluorescence observation toward a stage <NUM>. In the microscope apparatus <NUM>, the front of the stage <NUM> is covered with a removable sample cover <NUM>, and the upper portion of the stage <NUM> is covered with an openable/closable lid <NUM>, so that external light is prevented from entering the stage <NUM> during imaging by fluorescence observation.

In the microscope apparatus of <CIT>, the light source <NUM> emits light from above the stage <NUM>, and the light source <NUM> emits light from below the stage <NUM>. The optical axis of light from above irradiated from the light source <NUM> coincides with the optical axis of an objective lens <NUM> installed on the stage <NUM>.

Further, <CIT> is concerned with a box-type motor-operated microscope.

In the microscope apparatus <NUM> of <CIT>, external light may enter from a slight gap when the sample cover <NUM> and the lid <NUM> are not sufficiently adhered. Therefore, a problem arises inasmuch as it is difficult to accurately capture light when detecting and capturing a weak light. In addition, heat generated from the camera <NUM> or the like inside the sample cover <NUM> and the lid <NUM> is transmitted to the stage <NUM> and affects the sample.

Furthermore, in the microscope apparatus, it is desired to efficiently adjust the position of the sample for imaging the sample. It is also desired to prevent the contrast of an image of the sample from being weakened and to clearly capture the image of the sample when light is irradiated from above.

A microscope apparatus <NUM> according to a first aspect of the present invention is defined in claim <NUM> and comprises: a sample setting unit <NUM> in which a sample is set; an imaging unit 10d configured to image the sample set in the sample setting unit <NUM>; a housing unit <NUM> on which the sample setting unit <NUM> is arranged, and which is configured to internally accommodate the imaging unit 10d; a first light source <NUM> configured to irradiate light for fluorescence excitation on the sample in the sample setting unit <NUM>; and a first cover <NUM> configured to be movable to a light shielded first position that covers the sample setting unit <NUM> and an open second position that opens the sample setting unit <NUM>; characterized in that: the microscope apparatus further comprises: a second cover (<NUM> configured to be movable within the first cover <NUM> so as to be in a closed state that covers the sample setting unit <NUM> and an open state that opens the sample setting unit <NUM>; and a second light source <NUM> arranged in a space covered with the second cover <NUM> and configured to irradiate light on the sample in the sample setting unit <NUM>, and in that: the first cover <NUM> at the light shielded first position covers the second cover <NUM> in the closed state, wherein the second cover <NUM> is configured to cover the sample setting unit <NUM> so as to insulate the sample setting unit <NUM>.

In the microscope apparatus <NUM> according to the first aspect of the present invention described above is provided with the first cover <NUM> that is movable to the first position that covers the sample setting unit <NUM>, and the second cover <NUM> that is movable to the closed state that covers the sample setting unit <NUM> in the first cover <NUM>. In this way, since the sample setting unit <NUM> is covered twice by the first cover <NUM> and the second cover <NUM>, even if a slight gap is generated between the first cover <NUM> and the housing unit, the arrival of external light to the sample setting unit <NUM> can be reliably suppressed by the second cover <NUM>. As a result, it is possible to reliably suppress entry of light from the outside into the sample setting unit <NUM> and accurately capture weak light. Note that covering the sample setting unit <NUM> with the first cover <NUM> also includes the situation where the sample setting unit <NUM> is covered with the first cover <NUM> after covering the sample setting unit <NUM> with the second cover <NUM>, in addition to directly covering the sample setting unit <NUM> with the first cover <NUM>. Since the sample setting unit <NUM> can be covered by the second cover <NUM>, it also is possible to suppress the heat generated from the imaging unit 10d and the like disposed inside the housing unit <NUM> from being transmitted to the sample setting unit <NUM>. In this way the influence on the sample by heat can be reduced. By providing the second light source <NUM> that irradiates the sample setting unit <NUM> with light in a state in which the second cover <NUM> is closed, imaging can be performed with the first cover <NUM> and the second cover <NUM> closed, since the second light source <NUM> can irradiate the sample setting unit <NUM> in a closed state in which the external light does not reach the sample setting unit <NUM>. In this way, it is possible to perform imaging with fluorescence without performing the operation of closing the first cover <NUM> and the second cover <NUM> after adjusting the position of the sample for imaging the sample with light irradiated from the second light source <NUM>. As a result, it is possible to suppress the sample from being displaced due to vibrations when closing the first cover <NUM> and the second cover <NUM>, and it is possible to suppress an increase in imaging time.

In the microscope apparatus <NUM> according to the first aspect, the second light source <NUM> is preferably provided on the second cover <NUM>. If configured in this way, light can be easily irradiated on the sample setting part <NUM> from the second light source <NUM> in the state in which the second cover 22is closed. Further, it is not necessary to provide a light guide member such as an optical fiber, so that the configuration of illumination can be simplified by arranging the second light source <NUM> directly on the second cover <NUM>.

In this case, the second light source <NUM> preferably has a planar shape, a linear shape, or a punctate shape. If comprised in this way, the second light source <NUM> can be compactly arranged on the second cover <NUM> since the second light source <NUM> of thin planar shape, linear shape, or punctate shape can be arranged on the second cover <NUM>. In the case of surface emission, the amount of light also can be easily increased, so that clear imaging can be performed. In the case of linear light emission or punctate light emission, it is only necessary to arrange a needed amount of light emitters, so that the apparatus configuration can be simplified.

In the configuration in which the second light source <NUM> is provided on the side of the second cover <NUM> facing the sample setting unit <NUM>, the second cover <NUM> preferably surrounds the second light source <NUM> in a frame shape, and the sample setting unit <NUM> includes a recess <NUM> which accommodates the projection <NUM> when the second cover <NUM> is in a closed state. If configured in this way, the protrusion part <NUM> of the second cover <NUM> will enter into the concavity <NUM> of the sample setting unit <NUM>, such that the gap through which light enters directly between the second cover <NUM> and the sample setting unit <NUM> is suppressed and it is possible to more effectively suppress light from entering the sample setting unit <NUM>.

The microscope apparatus <NUM> according to the first aspect is preferably configured so that the second cover <NUM> is in a closed state that covers the sample setting unit <NUM> when the first cover <NUM> is located at the first position, and the second cover <NUM> is in an open state in which the sample setting unit <NUM> is exposed when the first cover <NUM> is located at the second position. If configured in this way, the sample setting unit <NUM> is covered twice by the first cover <NUM> and the second cover <NUM> by having the first cover <NUM> located at the first position (position which covers the sample setting unit <NUM>) and the second cover <NUM> located in a closed state. The sample setting unit <NUM> also can be easily accessed by placing the first cover <NUM> in the second position (open position) and opening the second cover <NUM>.

In this case, preferably, the second cover <NUM> is configured to be closed after the first cover <NUM> moves relative to the first position with regard to the housing unit <NUM>, and to be open before the first cover <NUM> moves relative to the second position with regard to the housing unit <NUM>. If configured in this way, since the first cover <NUM> does not relatively move when the second cover <NUM> is in the closed state, the closed second cover <NUM> does not interfere with the relative movement of the first cover <NUM>.

The microscope apparatus <NUM> according to the first aspect is preferably provided with a controller <NUM> for controlling the first drive unit 10a that moves the first cover <NUM> relative to the housing unit <NUM>, and the second drive unit <NUM> that drives the second cover <NUM> to open and close. If configured in this way, since the first cover <NUM> and the second cover <NUM> can be moved in concert by the controller <NUM>, the work burden of the user can be reduced compared with when the first cover <NUM> and the second cover <NUM> are moved manually.

In this case, the controller <NUM> is preferably configured to control the light irradiation of the first light source <NUM> and the light irradiation of the second light source <NUM>. If configured in this way, the light of the first light source <NUM> for fluorescence and the light of the second light source <NUM> can be switched by the controller <NUM>, and the sample setting unit <NUM> can be irradiated.

In the microscope apparatus <NUM> according to the first aspect, the second light source <NUM> preferably includes at least one of a halogen lamp, a tungsten lamp, a mercury lamp, a xenon lamp, and a light emitting element. If configured in this way, light can be irradiated on the sample setting unit <NUM> with a halogen lamp, a tungsten lamp, a mercury lamp, a xenon lamp, or a light emitting element.

In the microscope apparatus <NUM> according to the first aspect, the second light source <NUM> is configured to irradiate the sample with light from a direction oblique to the optical axis of the objective lens <NUM> provided in the sample setting unit <NUM>. If configured in this way, the sample can be imaged with augmented contrast compared with when light is irradiated in parallel with the optical axis of the objective lens <NUM>.

In the microscope apparatus <NUM> according to the first aspect, the second light source <NUM> is preferably configured to emit light for bright field. If configured in this way, the light for bright field is irradiated on the sample setting unit <NUM> by the second light source <NUM>, and bright field imaging is performed in the state in which the first cover <NUM> and the second cover <NUM> are closed.

In the microscope apparatus <NUM> according to the first aspect, preferably, a plurality of fluorescent images are captured by the imaging unit 10d using the fluorescence light of the first light source <NUM>, and a super-resolution image which exceeds the resolution of the imaging unit 10d is acquired based on the plurality of fluorescent images. If configured in this way, since the fluorescent image can be imaged by the imaging unit 10d in the state which external light is reliably prevented from entering the sample setting unit <NUM>, a super-resolution image can be imaged even with weak light.

A microscope apparatus <NUM> according to a second aspect is provided with a sample setting unit <NUM> for setting a sample, an imaging unit 10d for imaging a sample set on the sample setting unit <NUM>, a housing unit <NUM> within which the imaging unit 10d is disposed and provided with the sample setting unit <NUM>, a first light source <NUM> for irradiating the sample setting unit <NUM> with light for fluorescence, a first cover <NUM> which is movable between a first position covering the sample setting unit <NUM> and a second position exposing the sample setting unit <NUM>, and a second cover <NUM> that covers the sample setting unit <NUM> so as to insulate the sample setting unit <NUM> within the first cover <NUM>.

In the microscope apparatus <NUM> according to the second aspect described above, the first cover <NUM> is movable to a first position covering the sample setting unit <NUM>, and the second cover <NUM> is movable to close and cover the sample setting unit <NUM> within the first cover <NUM>. In this way, since the sample setting unit <NUM> can be covered twice by the first cover <NUM> and the second cover <NUM>, even if a slight gap is generated between the first cover <NUM> and the housing, the arrival of external light to the sample setting unit <NUM> can be reliably suppressed by the second cover <NUM>. As a result, it is possible to reliably suppress entry of light from the outside into the sample setting unit <NUM> and accurately capture weak light. Note that covering the sample setting unit <NUM> with the first cover <NUM> also includes the situation where the sample setting unit <NUM> is covered with the first cover <NUM> after covering the sample setting unit <NUM> with the second cover <NUM>, in addition to directly covering the sample setting unit <NUM> with the first cover <NUM>. Since the sample setting unit <NUM> can be covered by the second cover <NUM> so as to be thermally insulated, heat generated from the imaging unit 10d or the like disposed inside the housing unit <NUM> is prevented from being transmitted to the sample setting unit <NUM>. In this way the influence on the sample by heat can be reduced.

A microscope apparatus <NUM> according to a third aspect includes a sample setting unit <NUM> for setting a sample, an imaging unit 10d for imaging a sample set on the sample setting unit <NUM>, a first light source <NUM> that irradiates light from below on the sample setting unit <NUM>, a second light source <NUM> irradiates light from above on the sample setting unit <NUM>, wherein the second light source <NUM> is configured to irradiate light on the sample from an oblique direction with respect to an optical axis of an objective lens provided in the sample setting unit <NUM>.

In the microscope apparatus <NUM> according to the third aspect described above, the second light source 221is configured to irradiate the sample with light from a direction oblique to the optical axis of the objective lens <NUM> provided in the sample setting unit <NUM>. In this way the sample can be imaged with enhanced contrast compared with when light is irradiated in parallel with the optical axis of the objective lens <NUM>. As a result, a clear image can be obtained when imaged by light from above.

In the microscope apparatus <NUM> according to the third aspect, the second light source <NUM> is preferably arranged such that the optical axis is inclined with respect to the optical axis of the first light source <NUM>. If configured in this way, the optical axis direction of the second light source <NUM> can be inclined easily.

In the microscope apparatus <NUM> according to the third aspect, the microscope apparatus <NUM> preferably includes a cover <NUM> that covers the sample setting unit <NUM>, and the second illumination <NUM> is provided on the cover <NUM>. If configured in this way, light can be easily irradiated on the sample setting unit <NUM> from the second light source <NUM> when the cover <NUM> is closed. By arranging the second light source <NUM> directly on the cover <NUM>, it is unnecessary to provide a light guide member such as an optical fiber, so that the configuration of light source can be simplified.

In this case, the second light source <NUM> is preferably provided on the cover <NUM> so as to be inclined. According to this configuration, the optical axis direction of the second light source <NUM> can be easily inclined with respect to the optical axis direction of the objective lens <NUM>.

In the microscope apparatus <NUM> according to the third aspect, the second light source <NUM> is preferably formed so as not to irradiate light from a portion through which the optical axis of the first light source <NUM> passes. According to this configuration, the optical axis of the light of the second light source <NUM> can be easily shifted with respect to the optical axis of the objective lens <NUM>.

In the microscope apparatus <NUM> according to the third aspect, the first light source <NUM> preferably emits light for fluorescence excitation, and the second light source <NUM> preferably emits bright field light. If configured in this way, a bright field image can be captured clearly since the optical axis of the bright field light can be inclined.

In the microscope apparatus <NUM> according to the third aspect, the second light source <NUM> preferably has a planar shape, a linear shape, or a punctate shape. If configured in this way, the second light source <NUM> can be arrange compactly since a second light source <NUM> of thin planar shape, a linear shape, or a punctate shape can be used. In the case of surface emission, the amount of light also can be easily increased, so that clear imaging can be performed. In the case of linear light emission or punctate light emission, it is only necessary to arrange a needed amount of light emitters, so that the apparatus configuration can be simplified.

In the microscope apparatus <NUM> according to the third aspect, the second light source <NUM> preferably includes at least one of a halogen lamp, a tungsten lamp, a mercury lamp, a xenon lamp, and a light emitting element. If configured in this way, light can be irradiated on the sample setting unit <NUM> with a halogen lamp, a tungsten lamp, a mercury lamp, a xenon lamp, or a light emitting element.

The second and third aspects are examples, which do not fall under the scope of the claims.

It is possible to reliably suppress the entry of external light into the sample setting unit, accurately capture weak light, and suppress the influence of heat on the sample. A clear image also can be captured when imaging is performed by light from above.

An overview of the microscope apparatus <NUM> according to the present embodiment will be described with reference to <FIG>.

The microscope apparatus <NUM> is an apparatus for enlarging and displaying a sample placed on the sample setting unit <NUM>. The sample is a biological sample, such as cells, collected from a subject (specimen donor).

As shown in <FIG>, the microscope apparatus <NUM> includes a housing unit <NUM> and a first cover <NUM>. The microscope apparatus <NUM> includes an imaging unit 10d and a sample setting unit <NUM>. The imaging unit 10d includes an objective lens <NUM>, a first light source <NUM>, and an imaging element <NUM>. The sample setting unit <NUM> is provided on the upper surface (the surface on the Z1 direction side) of the housing unit <NUM>. The objective lens <NUM>, the first light source <NUM>, and the imaging element <NUM> are provided inside the housing unit <NUM>. The microscope apparatus <NUM> includes a display unit <NUM>. The display unit <NUM> is provided on the front surface (the surface on the Y1 direction side) of the first cover <NUM>. The display surface 21a of the display unit <NUM> is disposed on the front side of the first cover <NUM>. The microscope apparatus <NUM> includes a first drive unit <NUM> that moves the first cover <NUM> relative to the housing unit <NUM>. The microscope apparatus <NUM> includes a second cover <NUM>. The second cover <NUM> is disposed in the inside of the first cover <NUM>. The second cover <NUM> is provided with a second light source <NUM>.

Hereinafter, two directions orthogonal to each other in a plane parallel to the installation surface of the microscope apparatus <NUM> (that is, a horizontal plane) are defined as an X direction and a Y direction, respectively. As shown in <FIG>, the microscope apparatus <NUM> has a substantially rectangular outer shape that extends along the X direction and the Y direction in plan view. The X direction is the left-right direction of the microscope apparatus <NUM>, and the Y direction is the front-rear direction of the microscope apparatus <NUM>. The Y1 direction is the front direction of the apparatus main body, and the Y2 direction is the rear direction of the apparatus main body. The vertical direction perpendicular to the horizontal plane is designated the Z direction. The Z1 direction is the upward direction, and the Z2 direction is the downward direction.

The first cover <NUM> is relatively movable together with the display unit <NUM> with respect to the housing unit <NUM> to a first position (see <FIG>) at which the sample setting unit <NUM> is covered by the first cover <NUM>, and a second position (see <FIG>) at which the cover <NUM> is open and the sample setting unit <NUM> is exposed. Specifically, the first cover <NUM> is relatively movable to the first position (light shielded position) and the second position (open position) by sliding relative to the housing unit <NUM> in a direction substantially parallel to the installation surface of the housing unit <NUM>. The sample is set on the sample setting unit <NUM> in a state where the first cover <NUM> is relatively moved to the second position with respect to the housing unit <NUM>. The sample in the sample setting unit <NUM> is imaged with the first cover <NUM> relatively moved to the first position with respect to the housing unit <NUM>.

The imaging unit 10d images the sample placed in the sample setting unit <NUM>. Specifically, the imaging unit 10d collects light from the sample via the objective lens <NUM> and images the sample with the imaging element <NUM>. Light from the first light source <NUM> irradiates the sample and the imaging unit 10d captures an image by fluorescence. For example, the imaging unit 10d irradiates laser light from the first light source <NUM> to excite the sample, and images the fluorescence given off from the sample. That is, the imaging unit 10d captures a fluorescent image. Light from the second light source <NUM> irradiates the sample and the imaging unit 10d captures a bright field image. That is, when the first cover <NUM> and the second cover <NUM> are closed, it is possible to capture an image by irradiating light from the second light source <NUM> and to narrowly restrict an imaging region for performing fluorescence observation from the captured image. When the imaging region is narrowly restricted, it is possible to stop the irradiation of the light of the second light source <NUM> and continue to perform imaging by fluorescence observation since the first cover <NUM> and the second cover <NUM> are closed.

The sample setting unit <NUM> is provided in the housing unit <NUM>. The housing unit <NUM> includes an internal imaging unit 10d.

The first light source <NUM> irradiates the sample setting unit <NUM> with light for fluorescence excitation. For example, the first light source <NUM> irradiates the sample setting unit <NUM> with a laser beam of a specific wavelength. That is, the first light source <NUM> irradiates light for fluorescence excitation that excites the sample.

The second cover <NUM> is provided separately from the first cover <NUM>. The second cover <NUM> covers the sample setting unit <NUM> within the first cover <NUM>. The second cover <NUM> also is movable in the first cover <NUM> between a closed state that covers the sample setting unit <NUM> and an open state that exposes the sample setting unit <NUM>. The second cover <NUM> covers the sample setting unit <NUM> within the first cover <NUM> so as to insulate the sample setting unit <NUM>. That is, it is preferable that the second cover <NUM> is formed with a material which has thermal insulation properties. For example, the second cover may be formed of a heat insulating material such as an ABS resin or a PCABS resin, a metal provided with a heat insulating material, or the like.

The second light source <NUM> is provided separately from the first light source <NUM>. The second light source <NUM> can irradiate the sample setting unit <NUM> with light when the second cover <NUM> is closed. That is, the second light source <NUM> is disposed in the space covered by the second cover <NUM> and can irradiate the sample setting unit <NUM> with light. The second light source <NUM> emits light when performing bright field imaging. The second light source <NUM> does not irradiate light when performing fluorescence imaging.

As described above, the first cover <NUM> is provided so as to move to the first position that covers the sample setting unit <NUM>, and the second cover <NUM> is provided so as to cover the sample setting unit <NUM> in the first cover <NUM>. In this way, since the sample setting unit <NUM> can be covered twice by the first cover <NUM> and the second cover <NUM>, even if a slight gap is generated between the first cover <NUM> and the housing, the arrival of external light to the sample setting unit <NUM> can be reliably suppressed by the second cover <NUM>. As a result, it is possible to reliably suppress entry of light from the outside into the sample setting unit <NUM> and accurately capture weak light. Since the sample setting unit <NUM> can be covered by the second cover <NUM>, it also is possible to suppress the heat generated from the imaging unit 10d and the like disposed inside the housing unit <NUM> from being transmitted to the sample setting unit <NUM>. In this way the influence on the sample by heat can be reduced. By providing the second light source <NUM> that irradiates the sample setting unit <NUM> with light in a state in which the second cover <NUM> is closed, imaging can be performed with the first cover <NUM> and the second cover <NUM> closed, since the second light source <NUM> can irradiate the sample setting unit <NUM> in a closed state in which the external light does not reach the sample setting unit <NUM>. In this way, it is possible to perform imaging with fluorescence without performing the operation of closing the first cover <NUM> and the second cover <NUM> after adjusting the position of the sample for imaging the sample with light irradiated from the second light source <NUM>. As a result, it is possible to suppress the sample from being displaced due to vibrations when closing the first cover <NUM> and the second cover <NUM>, and it is possible to suppress an increase in imaging time.

As shown in <FIG>, the first cover <NUM> is substantially parallel to the installation surface of the housing unit <NUM> and is relatively slidable with regard to the housing unit <NUM> in the longitudinal direction (X direction) of the housing unit <NUM>. Specifically, the first cover <NUM> is moved with respect to the hosing <NUM> and the installation surface in a state wherein the housing unit <NUM> does not move with respect to the installation surface. The first cover <NUM> is configured to be movable relative to the housing unit <NUM> in a direction substantially parallel to the display surface 21a of the display unit <NUM>. In other words, the first cover <NUM> can be moved relative to the housing unit <NUM> in a direction (X direction) that is substantially perpendicular to a side surface (side surfaces in the X1 direction and the X2 direction) intersecting the front surface of the housing unit <NUM>. The first cover <NUM> also can be moved relative to the sample setting unit <NUM> in the horizontal direction. In this way enlargement of the microscope apparatus <NUM> in the vertical direction can be avoided compared with when the first cover <NUM> is moved orthogonally to the vertical direction with respect to the sample setting unit <NUM>.

The first cover <NUM> is moved relative to the housing unit <NUM> by the first drive unit 10a via external control. For example, the first cover <NUM> is relatively moved to the first position (light-shielding position) and the second position (open position) by driving the first drive unit 10a based on a user operation or a program. The first drive unit 10a includes, for example, a motor and a belt-pulley mechanism.

As shown in <FIG>, a sample is placed in the sample setting unit <NUM>. The sample setting unit <NUM> is disposed on the upper surface (surface in the Z1 direction) of the housing unit <NUM>, which is substantially parallel to the installation surface of the housing unit <NUM>. In this way, when the first cover <NUM> is relatively moved to the second position (open position), the upper part of the sample setting unit <NUM> can be opened, so that the sample setting part <NUM> can be easily accessed.

The sample setting unit <NUM> is provided in the housing unit <NUM> at a position lower than the horizontal surface 20a of the first cover <NUM>. In this way the upper part of the sample setting unit <NUM> can be opened, and the user can easily perform the sample setting operation on the sample setting unit <NUM> from above the sample setting unit <NUM>.

The sample setting unit <NUM> is provided in a concave shape on the upper surface of the housing unit <NUM> so that a portion, except for one side in the horizontal direction and the upper side, is circumscribed by a wall. For example, the sample setting unit <NUM> is provided in a concave shape on the upper surface of the housing unit <NUM> so that portions other than the front side and the upper side of the housing unit <NUM> are surrounded by a wall. Specifically, the sample setting unit <NUM> includes a wall part <NUM> provided in the Y2 direction and a wall part <NUM> arranged so as to face the X direction. The sample setting unit <NUM> is surrounded by the wall part <NUM> and a pair of wall parts <NUM> on the X1 direction side, the X2 direction side, and the Y2 direction side. When the first cover <NUM> is located at the second position (open position), the sample setting unit <NUM> is open on the upper side and in one horizontal direction. For example, when the first cover <NUM> is located at the second position, the sample setting unit <NUM> is open upward (Z1 direction) and forward (Y1 direction).

The sample setting unit <NUM> is disposed near the end of the housing unit <NUM> in the direction in which the first cover <NUM> moves relative to the housing unit <NUM>. The sample setting unit <NUM> is disposed on the upper surface near the end in the X direction of the housing unit <NUM>. As shown in <FIG>, the sample setting unit <NUM> is disposed in the vicinity of the end of the housing unit <NUM> on the X1 direction side. In this way, enlargement of the microscope apparatus <NUM> can be avoided when the first cover <NUM> moves to the second position since the first cover <NUM> is moved to the second position (open position) by moving the first cover <NUM> relative to the casing <NUM> by a length corresponding to the width of the sample setting unit <NUM>.

The sample setting unit <NUM> includes a stage 11a. The stage 11a is movable in the horizontal direction (X direction and Y direction) and in the vertical direction (Z direction). The stage 11a can move independently in the X direction, the Y direction, and the Z direction. In this way it is possible to enlarge and view a desired position of the sample since the sample can be moved relative to the objective lens <NUM>.

As shown in <FIG>, the objective lens <NUM> is disposed in the vicinity of the stage 11a of the sample setting unit <NUM>. The objective lens <NUM> is arranged close to the lower side (Z2 direction) of the stage 11a of the sample setting unit <NUM>. The objective lens <NUM> is provided so as to face the sample setting unit <NUM> in the vertical direction (Z direction). The objective lens <NUM> is arranged so that the optical axis is substantially perpendicular to the sample setting surface on which the sample is place on the sample setting unit <NUM>. The objective lens <NUM> is arranged facing upward. The objective lens <NUM> can be moved relative to the sample setting unit <NUM> in the vertical direction (Z direction). The objective lens <NUM> is disposed so as to have a longitudinal direction in the vertical direction. That is, the objective lens <NUM> is disposed so as to have an optical axis in a substantially vertical direction. The objective lens <NUM> includes a plurality of lenses. The objective lens <NUM> can enlarge the sample at a predetermined magnification. The objective lens <NUM> includes an immersion lens. That is, the objective lens <NUM> is used by dripping of oil such as silicone oil or liquid such as glycerin or water. Note that the objective lens <NUM> need not be an immersion lens. The objective lens <NUM> also may be used without dripping liquid.

As shown in <FIG>, the first light source <NUM> can irradiate light on the sample. The first light source <NUM> irradiates light on the sample through the objective lens <NUM>. The first light source <NUM> irradiates light on the sample from the same side as the imaging element <NUM>. The first light source <NUM> can output light having a predetermined wavelength. The first light source <NUM> can output light having a plurality of different wavelengths. That is, the first light source <NUM> can output different types of light. The first light source <NUM> includes a light emitting element. The light emitting element includes, for example, an LED element or a laser element.

As shown in <FIG>, the imaging element <NUM> can image a sample based on the light emitted from the first light source <NUM>. Specifically, the imaging element <NUM> can capture a still image or a moving image of the sample based on light from the sample irradiated by light emitted from the first light source <NUM>. The imaging element includes, for example, a CCD element and a CMOS element. The imaging element <NUM> can perform high-sensitivity imaging. That is, the imaging element <NUM> can capture an image based on weak light. The imaging element <NUM> images the sample based on the light of the second light source <NUM> provided on the side opposite to the objective lens <NUM> (Z1 direction side) with respect to the sample setting unit <NUM>.

As shown in <FIG>, the display unit <NUM> can display an image captured by the imaging element <NUM>. The display unit <NUM> is provided integrally with the first cover <NUM>. The display unit <NUM> can display a screen for operating the microscope apparatus <NUM>. The display unit <NUM> can display a screen based on a program for imaging a sample. The display unit <NUM> can display a screen indicating the state of the microscope apparatus <NUM>. The display unit <NUM> can display a screen based on a signal from an external control unit. The display unit <NUM> is disposed on one side of the first cover <NUM> in the horizontal direction. For example, the display unit <NUM> is disposed on the front side (Y1 direction side) of the first cover <NUM>.

As shown in <FIG>, the first cover <NUM> includes a horizontal surface 20a, an intersecting surface 20b, and a pair of side surfaces 20c arranged to face each other in the X direction. The horizontal surface 20a is configured to extend in a direction (XY direction) substantially parallel to the installation surface of the housing unit <NUM> so as to cover the sample installation unit <NUM> of the housing unit <NUM> from above. The intersecting surface 20b is connected to the horizontal surface 20a, extends in a direction intersecting the horizontal surface 20a, and is configured to cover the sample setting unit <NUM> of the housing unit <NUM> from one side substantially parallel to the setting surface. Specifically, the intersecting surface 20b is configured to cover the sample setting unit <NUM> of the housing unit <NUM> from the front. In this way, when the first cover <NUM> is relatively moved to the second position (open position), the upper side and the front side of the sample setting unit <NUM> can be opened, so that the sample setting unit <NUM> can be easily accessed. As a result, work on the sample setting unit <NUM> can be performed more easily. The visibility of the display unit <NUM> can be improved by positioning the display part <NUM> at the intersecting surface 20b since the display unit <NUM> can be arranged at the front surface. The side surface 20c is connected to the lower side of both ends in the X direction of the horizontal surface 20a. The side surface 20c is formed so as to extend in the vertical direction. The side surface 20c is configured to cover the sample setting unit <NUM> of the housing unit <NUM> from the X direction side. The first cover <NUM> is formed in a substantially inverted L shape by the horizontal surface 20a and the intersecting surface 20b. The display unit <NUM> is disposed on the intersecting surface 20b.

As shown in <FIG>, the first cover <NUM> is configured to substantially cover the entire housing unit <NUM> when the first cover <NUM> is located at the first position (light-shielding position), since the first cover <NUM> is substantially parallel to the installation surface of the housing unit <NUM>, that is, by the display unit <NUM> arranged on the intersecting surface 20b of the first cover <NUM> in the longitudinal direction of the housing unit <NUM>. The display unit <NUM> is disposed on substantially the entire intersecting surface 20b. The intersecting surface 20b is configured to cover the entire surface on one side in the horizontal direction of the housing unit <NUM> when the first cover <NUM> is located at the first position. The display unit <NUM> is disposed across substantially the entire intersecting surface 20b of the first cover <NUM> in the horizontal direction (X direction) of the screen. The display unit <NUM> is disposed across substantially the entire intersecting surface 20b of the first cover <NUM> in the vertical direction of the screen (the direction along the Z direction). In this way, since the display part <NUM> can be positioned in the range which covers substantially the entire longitudinal direction (X direction) of the front surface of the housing unit <NUM>, the display part <NUM> can be enlarged. As a result, it is possible to make the display contents easy to see.

The display unit <NUM> is arranged to have a predetermined inclination relative to a direction (Z direction) perpendicular to the installation surface of the housing unit <NUM>. In other words, the intersecting surface 20b of the first cover <NUM> is disposed so as to have a predetermined inclination relative to a direction (Z direction) perpendicular to the installation surface. For example, the display unit <NUM> is arranged in a state of being inclined by approximately <NUM> degree to <NUM> degrees relative to a direction perpendicular to the installation surface. The display unit <NUM> is arranged such that the lower end (Z2 direction end) protrudes forward (Y1 direction) relative to the upper end (Z <NUM> direction end). In this way the display part <NUM> can be made easier to see compared with when the display unit <NUM> is positioned along the direction perpendicular to the installation surface. The portion of the first cover <NUM> where the display unit <NUM> is disposed has substantially the same inclination as the predetermined inclination.

The display unit <NUM> is disposed on the first cover <NUM> so as to have a predetermined inclination relative to the vertical direction, and to move relative to the sample setting unit <NUM> with the display unit <NUM> arranged at the predetermined inclination. In this way the display unit <NUM> can be relatively moved in a state having a predetermined inclination, so that the display unit <NUM> can be easily seen at any position.

The front surface (surface in the Y1 direction) of the housing unit <NUM> has substantially the same inclination as the predetermined inclination of the intersecting surface 20b. The surface of the housing unit <NUM> facing the portion of the first cover <NUM> having substantially the same inclination as the predetermined inclination has substantially the same inclination as the predetermined inclination. The front surface of the housing unit <NUM> and the display unit <NUM> are substantially parallel.

The second cover <NUM> is closed to cover the sample setting unit <NUM> when the first cover <NUM> is located at the first position (light-shielding position), and the second cover <NUM> is open to expose the sample setting unit <NUM> when the first cover <NUM> is located at the second position (open position). In this way the sample cover <NUM> can be covered twice by the first cover <NUM> and the second cover <NUM> by placing the first cover <NUM> in the first position and closing the second cover <NUM>. The sample setting unit <NUM> also can be easily accessed by placing the first cover <NUM> in the second position and opening the second cover <NUM>.

Specifically, the second cover <NUM> is closed after the first cover <NUM> moves relative to the housing unit <NUM> to the first position (light shielding position), and the second cover <NUM> is open before the first cover <NUM> is moved to the second position (open position) relative to the housing unit <NUM>. That is, when the second cover <NUM> is in the open state, the first cover <NUM> moves relative to the housing unit <NUM>. In this way, when the second cover <NUM> is in the closed state, the first cover <NUM> is not relatively moved, so that the second cover <NUM> in the closed state is prevented from interfering with the relative movement of the first cover <NUM>.

The second cover <NUM> is attached to the inside of the side surface 20c on one side (X1 direction side) of the first cover <NUM>. The second cover <NUM> is rotatable around a rotational axis line extending in the Y direction. The second cover <NUM> enters the closed state which covers the sample setting unit <NUM> by rotating in a downward direction. The second cover <NUM> enters the open state in which the sample setting unit <NUM> is exposed by rotating in an upward direction. The second cover <NUM> may be switched between an open state and a closed state by sliding and moving in a horizontal direction. The second cover <NUM> also may be switched between an open state and a closed state by translational movement in a vertical direction.

The second cover <NUM> is driven relative to the first cover <NUM> by the second drive unit <NUM> under external control. For example, the second cover <NUM> is moved based on a user operation or a program such that the second drive unit <NUM> is driven to switch between a closed state and an open state. The second drive unit <NUM> includes, for example, a motor and a belt-pulley mechanism. The second cover <NUM> is driven by the second drive unit <NUM> in cooperation with opening and closing of the first cover <NUM>.

As described above, the sample setting unit <NUM> can be shielded from light during imaging by providing the first cover <NUM> which is movable relative to the housing unit <NUM> to the first position at which the sample setting unit <NUM> is shielded from external light (light-shielding position) and the second position at which the sample setting unit <NUM> is exposed (open position) relative to the housing unit <NUM>. In this way the microscope apparatus <NUM> can be installed and used in a bright location such as an examination room or a pathology classroom without installing the microscope apparatus <NUM> in a dark room. When the first cover <NUM> integrally provided with the display unit <NUM> is moved relative to the first position and the second position, the first cover <NUM> moves together with the display unit <NUM> so as to avoid blocking access to the sample setting unit when the first cover <NUM> is moved to the second position. In this way operations such as arranging a sample on the sample setting unit <NUM> can be easily performed. When the first cover <NUM> is moved to the second position, the display unit <NUM> does not get in the way when accessing the sample setting unit <NUM>, and the display unit <NUM> therefore can be maximally enlarged. In this way the enlarged and displayed sample can be confirmed in detail.

Next, a specific structural example of the microscope system <NUM> will be described with reference to <FIG>.

As shown in <FIG>, the microscope system <NUM> includes a microscope apparatus <NUM> and a control unit <NUM>. The microscope apparatus <NUM> and the control unit <NUM> are connected to each other so that signals can be transmitted and received. For example, the microscope apparatus <NUM> and the control unit <NUM> are connected to be communicable with each other by wire or wirelessly.

The control unit <NUM> is configured to control the microscope apparatus <NUM>. The control unit <NUM> is configured by a computer, for example, and includes a CPU (Central Processing Unit), a memory, and the like. The control unit <NUM> controls the sample imaging process performed by the microscope apparatus <NUM>. The control unit <NUM> controls the movement of the first cover <NUM> of the microscope apparatus <NUM> between the first position (light shielding position) and the second position (open position). The control unit <NUM> controls the movement of the second cover <NUM> of the microscope apparatus <NUM> between the closed state and the open state. The control unit <NUM> controls the microscope apparatus <NUM> based on a program. The control unit <NUM> can perform image processing on an image captured by the microscope apparatus <NUM>. The control unit <NUM> can output the processed image to the microscope apparatus <NUM> and display it on the display unit <NUM> of the microscope apparatus <NUM>. The control unit <NUM> can display an image based on the program on the display unit <NUM> of the microscope apparatus <NUM>.

Next, a specific structural example of the second cover <NUM> of the microscope apparatus <NUM> will be described with reference to <FIG> and <FIG>.

As shown in <FIG>, the second cover <NUM> is formed in a plate shape. As shown in <FIG>, the second cover <NUM> also is provided with a second light source <NUM> on the side of the second cover <NUM> that faces the sample setting unit <NUM>. In this way it is possible to easily irradiate light from the second light source <NUM> to the sample setting unit <NUM> with the second cover <NUM> closed. Since a light guide member such as an optical fiber is rendered unnecessary by arranging the second light source <NUM> directly on the side of the second cover <NUM> that faces the sample setting unit <NUM>, the illumination configuration can be simplified.

The second light source <NUM> includes a light emitter having a planar shape, a linear shape, or a punctate shape. In this way a thin light emitting body having a planar shape, a linear shape, or a punctate shape can be disposed on the second cover <NUM>, and the second light source <NUM> can be disposed on the second cover <NUM> compactly.

The second cover <NUM> includes a protrusion <NUM> that surrounds the second light source <NUM> in a frame shape and is formed to protrude toward the sample setting unit <NUM>. As shown in <FIG>, the sample setting unit <NUM> includes a concavity <NUM> into which the protrusion <NUM> is accommodated when the second cover <NUM> is in a closed state. Accordingly, the protrusion <NUM> of the second cover <NUM> enters the concavity <NUM> of the sample setting unit <NUM>, thereby suppressing a gap where light directly enters between the second cover <NUM> and the sample setting unit <NUM>, and light is more effectively suppressed from entering the sample setting unit <NUM>.

The second light source <NUM> is arranged so that the optical axis is shifted from the optical axis of the first light source <NUM>. In this way the optical axis of the first light source <NUM> can be directed in a direction suitable for imaging light from below by the first light source <NUM>, and the second light source <NUM> can be directed in the direction suitable for imaging light from above. In this way both the imaging by the light from above and the imaging by the light from below can be captured clearly.

For example, the second light source <NUM> is arranged such that the optical axis is inclined with respect to the optical axis of the first light source <NUM>. In this way light can be irradiated from the direction suitable for both light sources, respectively, since the optical axis direction of the first light source <NUM> and the optical axis direction of the second light source <NUM> can be shifted mutually.

The second light source <NUM> irradiates the sample with light from a direction oblique to the optical axis of the objective lens <NUM> provided in the sample setting unit <NUM>. That is, the second light source <NUM> is provided on the cover <NUM> so as to be inclined. In this way the sample can be imaged with enhanced contrast compared with when light is irradiated in parallel with the optical axis of the objective lens <NUM>. Note that the second light source <NUM> may be arranged so as to irradiate light parallel to the optical axis of the objective lens <NUM>.

The second light source <NUM> may include at least one of a halogen lamp, a tungsten lamp, a mercury lamp, a xenon lamp, and a light emitting element. When a halogen lamp, a tungsten lamp, a mercury lamp, or a xenon lamp is used as the second light source <NUM>, the light may be guided to the sample setting unit <NUM> by an optical fiber, a mirror, or the like.

The second light source <NUM> is formed so as not to irradiate light from a portion through which the optical axis of the first light source <NUM> passes. In this way the optical axis of the light of the second light source <NUM> can be easily shifted relative to the optical axis of the light of the first light source <NUM>. That is, the optical axis of the second light source <NUM> may be parallel to the optical axis of the first light source <NUM> as long as it is deviated from the optical axis of the first light source <NUM>.

For example, as shown in <FIG>, the second light source <NUM> may be provided with a light-opaque light blocking member <NUM> substantially at the center of the light emitter. In this way it is possible to suppress the light irradiated in parallel with the optical axis of the objective lens <NUM> from reaching the sample setting unit <NUM>. The light blocking member <NUM> is, for example, a light shielding seal. The light blocking member <NUM> is formed of a resin material or a metal material.

The second light source <NUM> may be provided in linear form on both sides of the center of the second cover <NUM>, as shown in <FIG>. As shown in <FIG>, the second light source <NUM> also may be provided in a rectangular circumferential shape so as to surround the center of the second cover <NUM>. The second light source <NUM> may be provided in circular periphery shape so that the center of the second cover <NUM> may be circumscribed, as shown in <FIG>. Note that the shape and arrangement of the second light source <NUM> need not be bilaterally symmetrical or point symmetrical.

Next, a structural example of the optical system of the microscope apparatus <NUM> will be described with reference to <FIG> and <FIG>.

As shown in <FIG>, the microscope apparatus <NUM> includes an objective lens <NUM>, a first light source <NUM>, an imaging element <NUM>, a first optical element <NUM>, a filter 16a, second optical elements 16b, 16c, 16f, and <NUM>, lenses 16d, 16e, <NUM>, reflectors 17a, 17b, and 17d, and a lens 17c. Objective lens <NUM>, first light source <NUM>, imaging element <NUM>, first optical element <NUM>, filter 16a, second optical elements 16b, 16c, 16f and <NUM>, lenses 16d, 16e, and <NUM>, reflectors 17a, 17b and 17d, and the lens 17c are disposed inside the housing unit <NUM>.

The first optical element <NUM> is configured to reflect the light emitted from the first light source <NUM> in the optical axis direction of the objective lens <NUM>, and transmit the light from the sample. The first optical element <NUM> includes, for example, a dichroic mirror. That is, the first optical element <NUM> is configured to reflect the light having the wavelength irradiated from the first light source <NUM>, and transmit the wavelength of the light generated from the sample.

The filter 16a is configured to transmit light of a predetermined wavelength and block light of other wavelengths, or to block light of a predetermined wavelength and transmit light of other wavelengths. In other words, light having a desired wavelength is transmitted by the filter 16a and reaches the imaging element <NUM>.

The second optical elements 16b, 16c, 16f, and <NUM> are configured to reflect light from the sample toward the imaging element <NUM>. The second optical elements 16b, 16c, 16f, and <NUM> include a reflector. The second optical elements 16b, 16c, 16f, and <NUM> include, for example, mirrors.

The reflectors 17a, 17b, and 17d are configured to reflect the light from the first light source <NUM> toward the objective lens <NUM>. The reflectors 17a, 17b, and 17d include, for example, a mirror.

The light emitted from the first light source <NUM> is reflected by the reflector 17a and enters the reflector 17b. The light that has entered the reflector 17b is reflected and enters the reflector 17d through the lens 17c. The light that has entered the reflector 17d is reflected and enters the first optical element <NUM>. The light incident on the first optical element <NUM> is reflected and reaches the sample setting unit <NUM> via the objective lens <NUM> and irradiates the sample.

The light emitted from the sample based on the light of the first light source <NUM> enters the first optical element <NUM> through the objective lens <NUM>. The light incident on the first optical element <NUM> is transmitted and enters the second optical element 16b via the filter 16a. The light incident on the second optical element 16b is reflected and incident on the second optical element 16c. The light incident on the second optical element 16c is reflected and enters the second optical element 16f via the lenses 16d and 16e. The light incident on the second optical element 16f is reflected and incident on the second optical element <NUM>. The light incident on the second optical element <NUM> is reflected and reaches the imaging element <NUM> via the lens <NUM>. The imaging element <NUM> captures an enlarged image of the sample based on the received light.

The first light source <NUM> is arranged at a position where the direction is changed at least once so that the light from the first light source <NUM> travels in a substantially vertical direction (Z direction) and enters the objective lens <NUM>. That is, the first light source <NUM> is arranged at a position offset relative to the optical axis of the objective lens <NUM>. In this way, when the objective lens <NUM> is arranged in a substantially vertical direction, it is not necessary to provide the first light source <NUM> on an extension line of the objective lens <NUM> in the optical axis direction, and thus an increase of size of the microscope apparatus <NUM> in the vertical direction is avoided.

The imaging element <NUM> is disposed at a position where the light from the sample is altered from a direction substantially parallel to the optical axis of the objective lens 12so as to enter the imaging element <NUM>. That is, the imaging element <NUM> is disposed at a position offset relative to the optical axis of the objective lens <NUM>. In this way, since it is unnecessary to provide the imaging element <NUM> on an extension line in the optical axis direction of light from the sample, it is possible to suppress an increase of the size of the microscope apparatus <NUM> fin the vertical direction. Note that the direction of the light from the sample need not be changed from the direction substantially parallel to the optical axis of the objective lens <NUM> until the light enters the imaging element <NUM>.

As shown in <FIG>, the microscope apparatus <NUM> includes a substrate <NUM> disposed inside the housing unit <NUM>, and on which the objective lens <NUM>, first light source <NUM>, and imaging element <NUM> are arranged so that the optical axis is substantially perpendicular to the sample setting unit <NUM>. The substrate <NUM> is positioned so as to be substantially perpendicular relative to the installation surface of the housing unit <NUM> (refer <FIG>). The substrate <NUM> is disposed so as to be substantially parallel to the optical axis of the objective lens <NUM>. Specifically, the substrate <NUM> is disposed so as to extend along the XZ plane. In this way, since the objective lens <NUM>, the first light source <NUM>, and the imaging element <NUM> can be arranged on the common substrate <NUM>, deviation of the positional relationship of the parts of the optical system can be suppressed.

The housing unit <NUM> has an internal space that extends in one direction. The objective lens <NUM> is arranged so that the optical axis is substantially perpendicular to the longitudinal direction (X direction) of the housing unit <NUM>. The first light source <NUM> and the imaging element <NUM> are arranged on the same side (X2 direction side) relative to the objective lens <NUM> in the longitudinal direction (X direction) of the housing unit <NUM>. In this way an increase of the size of the microscope apparatus <NUM> in the vertical direction can be suppressed.

The first optical element <NUM> and the second optical elements 16b, 16c, 16f, and <NUM> are disposed on the substrate <NUM>. In this way, it is possible to suppress displacement of the relative positional relationship between the element <NUM> and the second optical elements 16b, 16c, 16f, and <NUM> since the first light source <NUM>, the first optical element <NUM>, and the second optical elements 16b, 16c, 16f, and <NUM> can be arranged on the common substrate <NUM>.

The sample setting unit <NUM> is attached to the substrate <NUM> by both ends. That is, the sample setting unit <NUM> is supported by two pillars extending from the substrate <NUM> in the horizontal direction. In this way shifting of an imaging position at the time of imaging is suppressed since the sample setting part <NUM> can be supported stably.

Next, an example of a connection structure between the housing unit <NUM> and the first cover <NUM> of the microscope apparatus <NUM> will be described with reference to <FIG>.

As shown in <FIG>, the housing unit <NUM> includes an engaging part 10b that protrudes upward (Z1 direction). The first cover <NUM> includes a concavity <NUM> that engages with the engaging part 10b of the housing unit <NUM>. The concavity <NUM> is formed so as to be recessed in the vertical direction. The concavity <NUM> is formed to extend in the X direction. As shown in <FIG>, the concavity <NUM> of the first cover <NUM> engages with the engaging part 10b of the housing unit <NUM>. In this way the first cover <NUM> is connected to the housing unit <NUM> so that a movement in the X direction is possible.

As shown in <FIG>, the substrate <NUM> disposed inside the housing unit <NUM> includes a connection terminal <NUM>, a flex cable <NUM>, and a connection terminal <NUM>. The connection terminal <NUM> can be connected to the substrate <NUM>. The flex cable <NUM> connects the connection terminals <NUM> and <NUM> to each other. The connection terminal <NUM> can be connected to a substrate provided on the first cover <NUM>.

As shown in <FIG>, the display unit <NUM> is electrically connected to the housing unit <NUM> so as to be movable with respect to the housing unit <NUM>. In this way electrical power can be supplied to the display unit <NUM> which moves relatively with the first cover <NUM> with regard too the housing unit <NUM>, and electrical signals can be sent and received to/from the display unit <NUM>.

Next, a structural example of the controller <NUM> of the microscope apparatus <NUM> will be described with reference to <FIG>.

As shown in <FIG>, the microscope apparatus <NUM> includes a substrate <NUM>. The substrate <NUM> is provided with a power source <NUM>, a controller <NUM>, and a plurality of fans <NUM>. The substrate <NUM> is disposed below the interior of the housing unit <NUM> (see <FIG>). The substrate <NUM> is arranged so that it may become horizontal. The power source <NUM> is supplied with external power. The power source <NUM> supplies the supplied power to each part of the microscope apparatus <NUM>. For example, the power source <NUM> supplies power to the first light source <NUM>, the second light source <NUM>, the imaging element <NUM>, the display unit <NUM>, the first drive unit 10a, the second drive unit <NUM>, the controller <NUM>, the fan <NUM>, and the like.

The controller <NUM> controls each part of the microscope apparatus <NUM>. For example, the controller <NUM> controls light irradiation by the first light source <NUM>. The controller <NUM> controls the drive of the first drive unit 10a. The controller <NUM> controls light irradiation by the second light source <NUM>. The controller <NUM> controls the drive of the second drive unit <NUM>. The controller <NUM> controls each part of the microscope apparatus <NUM> based on control by the control unit <NUM>. The controller <NUM> is disposed inside the housing unit <NUM> in a region (see <FIG>) that is partitioned from a region where the objective lens <NUM>, the first light source <NUM>, and the imaging element <NUM> are disposed. Specifically, it is partitioned by a partition member 10c. A substrate <NUM> is disposed above the partition member 10c. A substrate <NUM> is disposed below the partition member 10c. In this way the controller <NUM> can be disposed separately from the objective lens <NUM>, the first light source <NUM>, and the imaging element <NUM>, so that heat generated by the controller <NUM> is not transmitted to the objective lens <NUM>, the first light source <NUM>, and the imaging element <NUM>. The light shielding property of the objective lens <NUM>, the first light source <NUM>, and the imaging element <NUM> can be enhanced by a member that partitions the region in which the controller <NUM> is disposed.

As shown in <FIG>, the fan <NUM> is configured to cool the inside of the housing unit <NUM>. Specifically, the fan <NUM> is configured to be driven to take in air from the outside into the housing unit <NUM>, circulate the intake air, and discharge the circulated air from the exhaust port 193a. A pair of fans <NUM> are provided along the X direction. The fan <NUM> is provided on the lower side (Z2 direction side) of the rear surface side (Y2 direction side) of the housing unit <NUM>. The operation of the fan <NUM> is stopped during the imaging of the sample by the imaging element <NUM>. In this way it is possible to prevent vibration caused by the fan <NUM> from being transmitted to the imaging element <NUM>, the sample setting unit <NUM> and the like during imaging, so that the sample can be imaged with high accuracy. Note that the fan <NUM> does not have to stop operation during imaging of the sample by the imaging device <NUM>. In this way the inside of the housing unit <NUM> can be efficiently cooled even during imaging.

As shown in <FIG>, the controller <NUM> is connected to the control unit <NUM>. The control unit <NUM> includes a processing unit <NUM>, a storage unit <NUM>, and an interface <NUM>. The control unit <NUM> is connected to the input unit <NUM>. The controller <NUM> is connected to the processing unit <NUM> via the interface <NUM>. The processing unit <NUM> includes, for example, a CPU and controls the operation of the microscope apparatus <NUM>. The storage unit <NUM> includes, for example, an HDD (hard disk drive), an SSD (solid state drive), and the like, and stores information and programs executed by the control unit <NUM>. The input unit <NUM> receives user operations. The input unit <NUM> includes, for example, a mouse and a keyboard. The input unit <NUM> is connected to the processing unit <NUM> via the interface <NUM>.

Next, the configuration of the microscope apparatus <NUM> according to a first modification will be described with reference to <FIG>.

As shown in <FIG>, the microscope apparatus <NUM> includes a housing unit <NUM> and a first cover <NUM>. The housing unit <NUM> is provided with a sample setting unit <NUM>. A display unit <NUM> is integrally provided on the first cover <NUM>. The sample setting unit <NUM> is provided with a second cover <NUM> that covers the sample setting unit <NUM>. As shown in <FIG>, the first cover <NUM> is disposed on the front surface side (Y1 direction side) of the housing unit <NUM>. The first cover <NUM> has a flat plate shape extending along a plane (XZ plane) perpendicular to the installation surface of the housing unit <NUM>.

The first cover <NUM> is configured to be movable between a first position (light shielding position) and a second position (open position) by sliding along the vertical direction (Z direction). The moving direction of the first cover <NUM> is substantially parallel to the plane direction in which the display unit <NUM> extends. That is, when the display unit <NUM> is arranged with a predetermined angle with respect to the vertical direction (Z direction), the moving direction of the first cover <NUM> is a direction inclined with a predetermined angle relative to the vertical direction (Z direction). As shown in <FIG>, when the first cover <NUM> is positioned at the second position, the front side (Y1 direction side) of the sample setting unit <NUM> is opened. In this case, the second cover <NUM> is also opened. The sample setting unit <NUM> is disposed on the X1 direction side of the housing unit <NUM>. The sample setting unit <NUM> is disposed on the upper side (Z1 direction side) of the housing unit <NUM> in the vertical direction (Z direction).

Next, with reference to <FIG>, the structure of the microscope apparatus <NUM> of a second modification is described.

As shown in <FIG>, the microscope apparatus <NUM> includes a housing unit <NUM> and a first cover <NUM>. The housing unit <NUM> is provided with a sample setting unit <NUM>. The first cover <NUM> is integrally provided with a display unit <NUM>. The sample setting unit <NUM> is provided with a second cover <NUM> that covers the sample setting unit <NUM>. As shown in <FIG>, the first cover <NUM> is disposed on the front surface side (Y1 direction side) of the housing unit <NUM>. The first cover <NUM> has a flat plate shape extending along a plane (XZ plane) perpendicular to the installation surface of the housing unit <NUM>.

The first cover <NUM> is configured to be movable between a first position (light-shielding position) and a second position (open position) by sliding along the horizontal direction (X direction). As shown in <FIG>, when the first cover <NUM> is located at the second position, the front side (Y1 direction side) of the sample setting unit <NUM> is opened. In this case, the second cover <NUM> is also opened. The sample setting unit <NUM> is movable in the forward direction (Y1 direction). Accordingly, when the sample setting unit <NUM> is moved forward, the upper side (Z1 direction) of the sample setting unit <NUM> is also opened. The sample setting unit <NUM> is disposed on the X1 direction side of the housing unit <NUM>. The sample setting unit <NUM> is disposed on the upper side (Z1 direction side) of the housing unit <NUM> in the vertical direction (Z direction).

Next, with reference to <FIG>, and <FIG>, the structure of the microscope apparatus <NUM> of a third modification is described.

As shown in <FIG>, the microscope apparatus <NUM> includes a housing unit <NUM> and a first cover <NUM>. The housing unit <NUM> is provided with a sample setting unit <NUM>. A display unit <NUM> is integrally provided on the first cover <NUM>. The sample setting unit <NUM> is provided with a second cover <NUM> that covers the sample setting unit <NUM>. As shown in <FIG>, the first cover <NUM> is disposed on the front surface side (Y1 direction side) of the housing unit <NUM>. The first cover <NUM> has a flat plate shape that extends along a plane (XZ plane) perpendicular to the installation surface of the housing unit <NUM>.

The first cover <NUM> is configured to be movable between a first position (light shielding position) and a second position (open position) by sliding along the horizontal direction (X direction). As shown in <FIG>, when the first cover <NUM> is located at the second position, the front side (Y1 direction side) of the sample setting unit <NUM> is opened. In this case, the second cover <NUM> is also opened. The sample setting unit <NUM> is disposed on the X1 direction side of the housing unit <NUM>. The sample setting unit <NUM> is disposed near the center of the housing unit <NUM> in the vertical direction (Z direction).

As shown in <FIG>, the objective lens <NUM>, the first light source <NUM>, the imaging element <NUM>, the actuator 611a, the first optical element <NUM>, the filter 16a, the second optical element 16b, and a lens <NUM> are provided on the substrate <NUM> of the microscope apparatus <NUM>. The objective lens <NUM> is disposed below (Z2 direction) the sample setting unit <NUM>. The sample setting unit <NUM> is disposed such that the distance D1 between the installation surface of the housing unit <NUM> and the sample setting unit <NUM> is longer than the length D2 of the objective lens <NUM> in the optical axis direction. In this way since the optical axis of the objective lens <NUM> can be arranged in the vertical direction (Z direction), the objective lens <NUM> can be easily brought near the sample in the optical axis direction when the sample setting unit <NUM> is set in the horizontal direction.

Next, a structural example of the first light source <NUM> will be described with reference to <FIG>.

As shown in <FIG>, the first light source <NUM> includes a first light source 131a for fluorescence excitation, a second light source 131b for fluorescence excitation, a mirror 132a, a dichroic mirror 132b, and a fan <NUM>. The first light source 131a and the second light source 131b output light having different wavelengths. The first light source 131a outputs light in a specific wavelength region. The second light source 131b outputs light in a specific wavelength region different from that of the first light source <NUM>1a. Each of the first light source 131a and the second light source 131b can output a laser beam. Note that the light output from the first light source <NUM>1a and the second light source 131b may be light in the visible light region, or may be light in the far infrared region, the near infrared region, the near ultraviolet region, or the far ultraviolet region or light in the invisible light region.

The light output from the first light source 131a is reflected by the mirror 132a, passes through the dichroic mirror 132b, and output from the first light source <NUM>. The light output from the second light 131b is reflected by the dichroic mirror 132b and output from the first light source <NUM>. In this way the light output from the first light source 131a and the light output from the second light source 131b are output from the first light source <NUM> such that the optical axes thereof are coincident with each other.

The first light source 131a irradiates the sample with light having a wavelength for activating a part of a plurality of dyes bonded to the sample. The second light source 131b irradiates the sample with light having a wavelength for deactivating the plurality of dyes that have been activated. The imaging element <NUM> is configured so that the light emitted from the one part of the stain which became activated among several stains may be imaged. In this way an image can be captured based on light emission of a part of the stain in an active state. The imaging element <NUM> is configured to image the sample a plurality of times. The display unit <NUM> is configured to display an image obtained by combining a plurality of images captured by the imaging element <NUM>.

Some of the stains bound to the sample emit light. The stain is bound to each cell molecule. The fluorescent image captured by sequential excitation of stains multiple times, that is, the fluorescence position of the stain, are acquired more accurately. Then, a plurality of images are superimposed. In this case, the fluorescence position of the stain is obtained with high accuracy in units of one molecule. By superimposing the fluorescent images acquired with the positional accuracy for each molecule, it is possible to acquire a super-resolution image exceeding the resolution limit.

The fan <NUM> is disposed inside the housing unit <NUM> and is provided to cool the first light source <NUM>. Specifically, the fan <NUM> is configured to generate an air flow around the first light source <NUM> when driven to remove heat generated from the first light source <NUM>. The operation of the fan <NUM> is stopped during the imaging of the sample by the imaging device <NUM>. In this way it is possible to prevent vibration generated by the fan <NUM> from being transmitted to the imaging element <NUM>, the sample setting unit <NUM> and the like during imaging, and thus it is possible to image the sample with high accuracy. Note that the fan <NUM> does not have to stop operating during imaging of the sample by the imaging element <NUM>. In this way it also is possible to cool the first light source <NUM> efficiently during imaging.

Next, an example of display screens displayed on the display unit <NUM> will be described with reference to <FIG>.

In the example of the display screen shown in <FIG>, when the sample is being imaged in the microscope apparatus <NUM>, the display for control and the display for analysis are displayed on the display unit <NUM>. The control display includes a camera screen display, an imaging parameter setting display, a sample setting unit moving operation display, an imaging parameter monitor display, and a first cover opening/closing operation display. The analysis display includes a super-resolution image display and a super-resolution image analysis parameter setting display.

In the camera screen display, a real-time camera screen imaged by the imaging element <NUM> is displayed. In the imaging parameter setting display, imaging parameters of the imaging process in the microscope apparatus <NUM> are displayed. In the imaging parameter setting display, for example, a display for adjusting the power of the laser beam output from the first light source <NUM> is displayed. For example, an operation screen for moving the position of the sample setting unit <NUM> is displayed on the sample setting unit moving operation display. Monitor information is displayed on the imaging parameter monitor display. In the imaging parameter monitor display, for example, the position of the sample setting unit <NUM>, the power of the laser light of the first light source <NUM>, the temperature of the imaging element <NUM>, the imaging time, the time until the end of imaging, and the like are displayed. In the first cover opening/closing operation display, for example, an operation screen for moving the first cover <NUM> to the first position (light shielding position) and the second position (open position) is displayed.

A super-resolution image is displayed in the super-resolution image display. Note that the data of the super-resolution image has a size of about several thousand pixels square to tens of thousands of pixels square. Here, it is preferable that the area of the display unit <NUM> is larger since the display area of super-resolution image display can be increased as the size of the display unit <NUM> is larger. In the super-resolution image analysis parameter setting display, analysis parameters for super-resolution imaging are displayed. In the super-resolution image analysis parameter setting display, for example, the irradiation order of the laser light output from the first light source <NUM> and the number of images to be captured are displayed.

Next, an example of an operation screen displayed on the display unit <NUM> will be described with reference to <FIG>. In the example of <FIG>, an example of an operation screen for moving the stage 11a of the sample setting unit <NUM> will be described. In the example of <FIG>, an operation button for moving the stage 11a in the X direction and the Y direction (horizontal direction) and an operation button for moving the stage 11a in the Z direction (vertical direction) are displayed. The user can move the stage 11a by operating each operation button. The stage 11a can be moved coarsely by operating the outer operation buttons. Moreover, the stage 11a can be moved finely by operating each operation button on the inner side. Note that the stage 11a can also be moved by operating an external keyboard or mouse.

The image capture process operation of the microscope system <NUM> will be described with reference to <FIG>.

First, when the imaging button is turned ON by user operation in step S <NUM> of <FIG>, then, in step S2, the control unit <NUM> performs controls to stop the driving of the fan <NUM> and the fan <NUM> via the controller <NUM>. In step S3, the control unit <NUM> controls imaging of the sample by the imaging element <NUM>. Imaging of the sample is performed multiple times. ! !br0ken!! For example, in step S3, the sample is imaged about several thousand to tens of thousands of times.

In step S4, after the imaging is finished, the control unit <NUM> performs control for driving the fan <NUM> and the fan <NUM> via the controller <NUM>. Thereafter, the image capturing process operation is terminated.

The super-resolution image creation process operation of the microscope system <NUM> will be described with reference to <FIG>.

First, in step S11 of <FIG>, the control unit <NUM> images the fluorescence of the sample while irradiating light for fluorescence excitation from the first light source <NUM>. In step S12, the control unit <NUM> extracts a fluorescent spot of each captured image. Specifically, fluorescent spots are extracted from the captured image by Gaussian fitting. In step S <NUM>, the control unit <NUM> acquires the coordinates of the extracted spot. That is, the position of the pixel of the bright spot on the image is obtained. Specifically, the coordinates of each spot are acquired on a two-dimensional plane. Then, a bright spot region on the image is acquired. Specifically, regarding each fluorescent region on the captured image, each bright spot region of a breadth corresponding to a range is allocated to each bright spot when matching with a reference waveform within a predetermined range is obtained by Gaussian fitting. A bright spot region having the lowest level is assigned to the bright spot in the fluorescent region that matches the reference waveform at one point.

In step S14, the control unit <NUM> overlaps the bright spot areas of the images. Then, the control unit <NUM> creates a super-resolution image by superimposing the acquired bright spot region of each bright spot on all the images. Thereafter, the super-resolution image creation process is terminated.

Claim 1:
A microscope apparatus comprising:
a sample setting unit (<NUM>) in which a sample is set;
an imaging unit (10d) configured to image the sample set in the sample setting unit (<NUM>);
a housing unit (<NUM>) on which the sample setting unit (<NUM>) is arranged, and which is configured to internally accommodate the imaging unit (10d);
a first light source (<NUM>) configured to irradiate light for fluorescence excitation on the sample in the sample setting unit (<NUM>); and
a first cover (<NUM>) configured to be movable to a light shielded first position that covers the sample setting unit (<NUM>) and an open second position that opens the sample setting unit (<NUM>); characterized in that: the microscope apparatus further comprises:
a second cover (<NUM>) configured to be movable within the first cover (<NUM>) so as to be in a closed state that covers the sample setting unit (<NUM>) and an open state that opens the sample setting unit (<NUM>); and
a second light source (<NUM>) arranged in a space covered with the second cover (<NUM>) and configured to irradiate light on the sample in the sample setting unit (<NUM>), and in that: the first cover (<NUM>) at the light shielded first position covers the second cover (<NUM>) in the closed state,
wherein the second cover (<NUM>) is configured to cover the sample setting unit (<NUM>) so as to insulate the sample setting unit (<NUM>).