Patent Description:
According to Law No. <NUM> of <NUM>/<NUM>/<NUM> laying down rules on hygiene in the production and trade of beer, the designation as "beer" is reserved for the product obtained from the alcoholic fermentation with strains of Saccharomyces carlsbergensis or Saccharomyces cerevisiae of a wort prepared with malt, whether or not roasted, of barley or wheat or mixtures thereof and water, bittered with hops or derivatives thereof, or both. Furthermore, according to the same Law, barley or wheat malt - which is more expensive in that it already contains the set of enzymes necessary for saccharification, i.e., the transformation of starch into fermentable sugars - can be replaced with other cereals, whether or not broken or ground or in the form of flakes, as well as with starchy and sugary raw materials which generally are less expensive than malt. In addition to economic reasons, the replacement of malt with unmalted cereals and other substitutes meets the need to diversify the "beer" product by imparting organoleptic characteristics different from those of conventional beers.

Cereals such as wheat, rye, barley, oats, spelt, kamut or hybridised strains thereof and derivative products are known to contain proteins called gliadins which, together with glutenins, form the viscoelastic complex known as gluten. By way of example, Table <NUM> below shows the protein and gluten protein contents of some cereals (<NPL>;<NPL>; <NPL>:.

Celiac disease is an autoimmune disease that affects genetically predisposed individuals when they consume gluten-containing foods and for which gliadin is responsible. In predisposed individuals, the intake of gluten-containing foods may lead to intestinal symptoms (diarrhoea, abdominal pain and distension, vomiting, flatulence) or extra-intestinal symptoms (anaemia, rickets, osteoporosis, lack of concentration, development of other autoimmune diseases, vitamin and mineral deficiencies with all the resulting diseases). The autoimmune basis of the disease, when combined with the introduction of even minimal amounts of gluten, leads to inflammatory processes which cause atrophy, in the most serious cases permanent atrophy, of the intestinal villi, making it difficult to absorb nutrients.

There are currently no treatments for celiac disease and those who suffer from it must necessarily completely exclude gluten from their diet. Celiacs must therefore use foods in their diet that are specifically produced, prepared and/or processed a) to reduce the gluten content in one or more gluten-containing ingredients, b) to replace gluten-containing ingredients with other ingredients that are naturally gluten-free. Generally, since the removal of gluten from cereals containing it exhibits significant technical difficulties and economic constraints, the second option is preferred.

Beers produced from traditional raw materials therefore have gluten contents generally exceeding <NUM>/kg by weight, a value chosen on a preventive basis as a safety threshold in order to prevent adverse effects caused by high gluten ingestion and laid down in Community legislation for the labelling of gluten-free products. In fact, according to Commission Implementing Regulation (EU) No. <NUM>/<NUM>, the term 'gluten-free' is only allowed where the gluten content in the food sold to the final consumer does not exceed <NUM>/kg by weight.

In addition, it should be noted that the gluten content in craft beers may be higher than that of industrial beers (<NPL>), since the Italian legislation establishes that craft beer, during production, is not subjected to microfiltration steps (Law No. <NUM>, art. <NUM>, paragraph 4a of <NUM>/<NUM>/<NUM>) which, in addition to clarifying the beer, allow the removal of a large part of the protein component.

Several gluten-free "beers" are available on the market, which are produced by replacing barley or wheat malt (and other cereals, whether or not malted, containing gluten protein) with substitute starchy sources such as gluten-free cereals (rice, maize, millet), pseudocereals (quinoa, buckwheat) or other ingredients capable of providing fermentable sugars, such as chestnuts or pumpkin. However, according to the aforementioned Italian legislation on beer production, barley and/or wheat cannot be replaced for more than <NUM>% of the dry wort extract with other cereals or starchy and sugary substances, and therefore if only gluten-free raw materials are used as the raw material, the final product cannot be defined as "beer", but as "alcoholic beverage based on". Furthermore, these gluten-free "beers" have organoleptic characteristics that are significantly different from those of beers produced with conventional ingredients (<NPL>) - changes often unwelcome by regular consumers - and also increased susceptibility to drop in quality. By way of example, rice beer generally has low alcohol content, poor froth stability, lack of body (ELM and Brenna OV strains; "<NPL>) and, due to the higher lipid content in rice compared to barley, greater susceptibility to oxidation of free unsaturated fatty acids and to the development of rancid notes (<NPL>).

<NPL> discloses a process of producing a gluten-free beer with a mixture of a plurality of gluten-containing cereal grains with water.

<CIT> discloses a method to produce a beer with an increased level of free amino acid content comprising adding grists from malted gluten containing cereals together with adjuncts of non-malted cereals and comprising the step of boiling the hops with the wort for <NUM>.

<NPL>" discloses a step of adding a heating step when using unmalted barley.

If beer is not a staple food, it should be noted that most of the gluten introduced in the diet is that contained in cereal derivatives such as pasta and baked goods. In this case, too, wheat flours are replaced by rice flour, white and yellow maize, pulses (beans, lentils, lupines, soya beans), buckwheat and, again, the use of gluten-free raw materials leads to substantial organoleptic changes as well as technological problems due to the need to compensate for the absence of gluten. In fact, the gluten lattice, which is formed only following the hydration of the semolina/flour and the kneading of the dough, traps the starch granules, thus conferring resistance to the pasta when it is cooked and preventing excessive leaching of the amylose into the cooking water, and at the same time retains the carbon dioxide bubbles that develop during bread leavening, thus allowing the formation of an alveolate and compact texture (the crumb). Gluten properties are linked to the ability of glutenins to confer elasticity and toughness and to the ability of gliadins to confer extensibility and viscosity to the dough. In the absence of gluten, the structuring function of gluten is partially replaced by modifying the formulations by adding thickeners, emulsifiers, or pre-gelatinised starches.

Spent grains represent the most abundant by-product of the brewing industry (<NUM>% of the total) and it has been calculated that the brewing industry annually produces about <NUM> million tons of them in the European Union alone. Spent grains consist of the husk (glumella), which initially covers the barley grain, the pericarp, and the seed coverings, which remain after the mashing step.

Disposal of the spent grains is very expensive. Currently, spent grains can be partially used in animal feed, in particular in an amount not exceeding <NUM>% of the dairy cows' daily ration, because their high content of moisture (about <NUM>-<NUM>%) and nutrients on the one hand makes them susceptible to microbial growth and, on the other, makes their storage and transport uneconomical.

However, the analysis of the composition of spent grains has opened up interesting prospects for the use of this by-product as food. Their complete composition, on a dry matter basis, is as follows: dietary fibre <NUM>-<NUM>%, of which <NUM>-<NUM>% insoluble and <NUM>-<NUM>% soluble; protein <NUM>-<NUM>%; sugars <NUM>-<NUM>%; lipids <NUM>-<NUM>%; ash <NUM>-<NUM>% (the most represented minerals include phosphorus, calcium, magnesium, and sodium, representing <NUM>, <NUM>, <NUM> and <NUM>/kg, respectively); tocopherols, tocotrienols, and B vitamins; phenolic compounds, mainly ferulic acid (<NUM>/kg dry matter) and p-coumaric acid (<NUM>/kg dry matter) (<NPL>).

Due to their high nutritional value and the ability to turn them into flour, spent grains represent a raw material suitable for use in food formulations that include a kneading step in the production process (pasta, bread, snacks, biscuits, and other baked goods). However, their gluten content is higher than that laid down in Community legislation for products labelled as gluten-free, preventing their use in food production for celiacs.

Therefore, one object of the present invention is to provide a method which allows gluten-free beer to be produced on a large scale, with a limited number of operating steps, starting from gluten-containing cereals, while retaining the main organoleptic characteristics of conventional beers.

Another object of the invention is to provide a method capable of obtaining gluten-free spent grains, while preserving their high nutritional value, which are suitable for use in human food production, in particular of flours and derivatives thereof.

In consideration of these objects, a method of producing gluten-free beer, a method of producing gluten-free spent grains, and a method of producing gluten-free flour form the object of the invention, as defined in the attached independent claims.

Further features and advantages of the invention are identified in the attached dependent claims and illustrated in detail in the following description.

The attached independent and dependent claims form an integral part of the present specification.

As will be apparent from the following detailed description, the present invention provides a method of producing gluten-free beer which is defined by a combination of operating conditions which, while starting from raw materials with a high gluten content, surprisingly allow a controlled degradation of the beer protein fraction, more specifically of the gluten protein complex, without however affecting the qualitative properties of this beverage, in particular the formation and consistency of the froth.

Advantageously, therefore, the method which forms the object of the invention allows a gluten-free beer or a beer with an extremely low gluten content to be obtained, without the need for detoxification procedures which require the use of exogenous enzymes or preliminary grain treatments.

Therefore, one object of the present invention is a method of producing gluten-free beer, wherein the method employs a mixture of a plurality of gluten-containing cereal grains as the starting material and comprises the steps of:.

characterised in that the mixture of a plurality of gluten-containing cereal grains employed as the starting material comprises barley malt in an amount of between <NUM>% and <NUM>% by weight and one or more unmalted cereals in a total amount of between <NUM>% and <NUM>% by weight.

The term "gluten-free beer", as used herein, refers to a beer beverage containing gluten in a total amount of less than <NUM> milligrams of gluten per kilogram of beer (< <NUM>/kg).

The term "spent grains", as used herein, refers to the solid residue of the wort consisting of the outer shells of the malted cereal grains and other parts that have not undergone solubilisation in the mashing process.

The term "gluten-free spent grains", as used herein, refers to spent grains containing gluten in a total amount of less than <NUM> milligrams of gluten per kilogram of spent grains (< <NUM>/kg).

Within the scope of the present description, the term "barley malt" refers to the product obtained by subjecting the barley kernel (grain) to the malting process, i.e., the induction of kernel germination followed by drying. As is well known in the art, malting enables starch reserves in the barley kernel to be transformed into simple fermentable sugars.

The term "unmalted cereals", as used herein, refers to cereal grains which have not undergone the malting process.

The method which forms the object of the invention is based on a sequence of successive steps carried out at predetermined temperatures and for specified periods of time, wherein steps (i) to (iv), also known as the mashing, are responsible for the production of a wort containing fermentable sugars, step (v) concerns the boiling of the wort and its aromatization and bittering with hops, and step (vi) provides for the alcoholic fermentation of the aforementioned hop-treated wort.

According to the invention, the starting material employed in the method consists of a mixture of a plurality of gluten-containing cereal grains, which comprises barley malt in an amount of between <NUM>% and <NUM>% by weight, e.g., <NUM>%, <NUM>%, <NUM>%, or <NUM>% by weight, and one or more unmalted cereals in a total amount of between <NUM>% and <NUM>% by weight, e.g., <NUM>%, <NUM>%, <NUM>%, or <NUM>% by weight.

In a preferred embodiment, the mixture employed as the starting material in the method of the invention consists of barley malt in an amount of between <NUM>% and <NUM>% by weight, e.g., <NUM>%, <NUM>%, <NUM>%, or <NUM>% by weight, and one or more unmalted cereals in a total amount of between <NUM>% and <NUM>% by weight, e.g., <NUM>%, <NUM>%, <NUM>%, or <NUM>% by weight.

Preferably, the one or more unmalted cereals in the mixture according to the invention are selected from the group consisting of soft wheat, durum wheat, spelt, and any combination thereof.

Optionally, the plurality of cereal grains in the mixture according to the invention are wholly or partly in the form of ground grains. In this form, the contact surface between the starchy raw material and the water is increased, thereby advantageously increasing the efficiency of the sugar extraction process and the solubilisation of the sugars during the production of the wort (mashing).

The grinding of the plurality of cereal grains in the mixture is preferably coarse so that, at the end of the mashing, the residual solid fraction (spent grains) deposits on the bottom of the mashing tun and acts as a filtering element for the sugary wort. In fact, too much grinding has the disadvantage of forming particles that remain suspended in the sugary wort.

The selection of the most suitable cereal grain grinding techniques for use within the scope of the present invention falls well within the skills of those of ordinary skill in the art.

Preferably, in step (i) the water temperature is between <NUM> and <NUM>, e.g., <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM>.

According to the invention, a water temperature in the above range favours the extraction and subsequent solubilisation of the substances contained in the plurality of cereal grains employed as the starting material, including the various enzymes occurring in barley malt.

Preferably, in step (ii) the aqueous mixture is kept at a temperature of <NUM> for between <NUM> and <NUM> minutes, e.g., <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM> minutes.

Step (ii), also called "protein rest", and subsequent step (iii) of the method of the invention, respectively, enable the activation of protease and amylolytic enzymes contained in barley malt and the consequent degradation of protein and starch molecules occurring in the cereal grains of the starting material.

Advantageously, the present inventors have found that, due to the particular combination of the operating temperature and time parameters which characterize the mashing step of the method according to the invention, a partial protein degradation occurs in the aqueous mixture resulting in the elimination or in an extremely significant reduction of the gluten protein complex, without at the same time impairing the formation of froth in the final product and/or changing the consistency and persistence thereof. As is well known in the art, froth is indeed a fundamental characteristic of beer, as it can retain scents, delay oxidation, and reduce carbon dioxide evaporation when the beverage is poured.

In one embodiment, step (iii) of the method according to the invention comprises:.

In the above embodiment, step (a) activates the beta-amylase enzyme, which acts by hydrolyzing the starch chains starting from the ends of these molecules, resulting in the production of maltose and dextrins, whereas in step (b) the alpha-amylase enzyme, on the other hand, catalyzes the cleavage of glucosidic bonds in the starch molecule at random points along the chain.

In another embodiment, the method according to the invention further comprises, after step (iii) and before step (iv), a step of further heating the wort to a temperature comprised between <NUM> and <NUM> and keeping it at said temperature for at least <NUM> minutes, preferably <NUM> - <NUM> minutes. A temperature of <NUM> or <NUM> is particularly preferred.

The high temperature of the above-mentioned step advantageously leads to the degradation of the enzyme molecules contained in the wort, thereby blocking and inhibiting the enzymatic reactions mediated by the same.

Preferably, step (iv) of filtering the wort is carried out by recovering the wort after the passage through the spent grains deposited on a sieve lying on a perforated stainless-steel support.

Preferably, step (v) of boiling the clear wort together with hops at a temperature of <NUM> takes place for between <NUM> and <NUM> minutes, more preferably between <NUM> and <NUM> minutes.

There are several advantages brought about by step (v) of boiling with hops, the so-called hopping, including denaturation of the protein substances contained in the wort, including enzyme molecules, also due to the formation of tannin-protein complexes, resulting in the precipitation of these substances and clarification of the wort; sterilization of the wort and its concentration by evaporation; increased reducing power of the wort mediated by the production of melanoidins and its flavouring.

According to one embodiment of the method of the invention, at the end of the boiling step, the wort is cooled to a temperature comprised between <NUM> and <NUM> and subjected to a method for removing the impurities due to hop residues and coagulated proteins.

Methods suitable for use in the aforementioned purification step are known and described in the state of the art, therefore the selection and use thereof are well within the skills of those of ordinary skill in the art. As a non-limiting example, the whirlpool procedure is mentioned, i.e., a method of circular movement of the wort that favours the decanting of the solid parts in a single central area of the tun where the wort is temporarily placed.

Preferably, the one or more yeasts belonging to the Saccharomyces genus employed in the fermentation step (vi) are selected from either Saccharomyces cerevisiae or Saccharomyces carlsbergensis.

In one embodiment, the method according to the invention comprises subjecting the gluten-free beer obtained in step (vi) to further fermentation, after bottling.

Preferably, the further fermentation step in the bottle comprises the steps of adding sucrose sugar to the gluten-free beer, more preferably in a concentration by weight comprised between <NUM>% and <NUM>% w/v based on the total volume of the beer, and inoculating in said beer the Saccharomyces cerevisiae yeast at a dose preferably comprised between <NUM><NUM> and <NUM><NUM> colony-forming units per millilitre of beer (cfu/ml).

This so-called "re-fermentation" step in the bottle, by favouring the trapping in the bottle, until uncorking, of the carbon dioxide developed, advantageously allows the beverage stability to be enhanced and the degree of effervescence and the amount of froth to be increased.

The preferred embodiments of the method according to the invention described above can be combined with each other as required, and the implementation of these combinations falls within the skills of the person skilled in the art.

A method of producing gluten-free spent grains, comprising the sequence of steps (i) to (iv) as defined above in relation to the method of producing gluten-free beer and using the same starting material as the aforementioned method, also falls within the scope of the invention.

It is understood that the embodiments described above in relation to the method of producing gluten-free beer also apply to the method of producing gluten-free spent grains according to the invention.

As will be explained in more detail in the following experimental section, surprisingly, the method of the present invention makes it possible to obtain spent grains characterized by an extremely low gluten content, in a total amount of less than <NUM>/kg by weight, but with a particularly high content of antioxidants, especially phenolic compounds, which makes them particularly suitable to be used for food consumption by people suffering from celiac disease.

Therefore, the use of gluten-free spent grains obtainable with the method which forms the object of the invention, for the production of human food products, is also described.

Preferred human food products are selected from the group consisting of flour, bread, pasta, piade, piadine, pinse, pizza, frise, friselle, croutons, taralli, scaldatelli, biscuits, sweet cakes, savoury cakes, and any combination thereof.

A further object of the invention is a method of producing gluten-free flour, comprising the step of carrying out the method as previously defined to obtain gluten-free spent grains, and the subsequent steps of drying the gluten-free spent grains thus obtained and grinding the dried gluten-free spent grains.

Preferably, the drying step is carried out at room temperature, even more preferably by forced ventilation, for example up to a moisture content of less than <NUM>%.

Preferably, the grinding of the dried spent grains is carried out by using a knife mill, with heat dissipation.

The examples that follow are provided for illustration purposes and do not limit the scope of the invention as defined in the appended claims.

Some operating examples of the method of producing gluten-free beer and of the method of producing gluten-free spent grains forming the object of the present invention are given below.

The barley malt normally used in brewing has a total protein content ranging from <NUM>% to <NUM>% by weight.

In order to perform the method according to the invention under extreme conditions in terms of gluten content, mixtures of a plurality of cereal grains having the following composition were sent for brewing:.

The above described mixtures of barley malt and unmalted cereals were coarsely ground and then mashed under the following conditions:.

Subsequently, the sparging of the spent grains was carried out by using water at a temperature of <NUM>. Within the scope of the present description, the term "sparging of the spent grains" refers to the washing of the spent grains after filtration and the collection of the resulting solution in order to maximize sugar recovery. The temperature is established so as to avoid the extraction of tannins, which would give an unpleasant bitter taste, different from that given by hops.

The wort was then boiled at a temperature of <NUM> for <NUM> minutes for worts obtained from the cereal mixtures b) and c) or at a temperature of <NUM> for <NUM> minutes for worts obtained from the cereal mixtures a) and b).

The final wort density was in the range of <NUM> to <NUM> for worts obtained from the cereal mixtures b) and c), whereas the wort density value was <NUM> for worts obtained from the cereal mixtures a) and b).

During the boiling of the wort, the following were added:.

After whirlpooling and cooling, the worts were inoculated with commercial strains of Saccharomyces cerevisiae (approximately <NUM><NUM> cfu/ml) and fermented as follows: an initial tumultuous fermentation at <NUM>, followed by racking and slow fermentation at <NUM>-<NUM> until constant density values of around <NUM> were reached. Subsequently, the beers were bottled and subjected to a third fermenting step (re-fermentation in the bottle):.

The beers thus produced have not undergone filtration and pasteurization treatments.

It is clear from <FIG> that the gluten-free beer produced with the method of the invention has a satisfactory amount of froth (positive evaluation element).

The method of the invention as described in the preceding paragraph up to the sparging of the spent grains was followed for the production of gluten-free spent grains.

The gluten-free spent grains thus recovered, shown in <FIG>, which had a moisture content of approximately <NUM>%, were dried under forced ventilation at <NUM>-<NUM> until moisture values of less than <NUM>% were reached.

The dried gluten-free spent grains, as shown in <FIG>, were ground by using a knife mill, resulting in the production of flours (<FIG>) having the particle size distribution shown in Table <NUM> below:.

The gluten-free spent grain flours obtained with the method of the invention were used to produce bread by replacing <NUM>% of commercial gluten-free flour with these flours and applying a <NUM>% hydration level.

<FIG> shows the section of a piece of bread made from <NUM>% gluten-free spent grain flour resulting from the method of the invention employing a mixture of barley malt and soft wheat as the starting material.

For the preparation of biscuits, the present inventors replaced <NUM>% ("biscuits <NUM>%") and <NUM>% ("biscuits <NUM>%") of commercial gluten-free flour with the gluten-free spent grain flours produced with the method of the invention and added other ingredients such as eggs, olive oil, chemical yeast, and flavourings. The biscuits were baked in the oven at <NUM> for <NUM>-<NUM> minutes depending on the thickness. <FIG>, respectively, show biscuits produced by replacing commercial gluten-free flour with <NUM>% and <NUM>% gluten-free spent grain flour resulting from the method according to the invention employing a mixture of barley malt and soft wheat as the starting material. The biscuits in <FIG> have a thickness of about <NUM> and were baked at <NUM> for <NUM> minutes.

Tables <NUM> to <NUM> below show the physicochemical characteristics and gluten content of gluten-free beers and gluten-free spent grains, respectively, produced with the method according to the invention, as well as, by way of example, the physicochemical characteristics and gluten content of bread and biscuits produced with the flours from said gluten-free spent grains. The data are shown as mean ± standard deviation.

The data show gluten contents below the detection threshold of the method applied for beers and in any case far below the threshold value of <NUM>/kg by weight for spent grains and related products. It should be noted that the gluten-free beers, spent grains, bread, and biscuits produced with the methods forming the object of the invention also have high contents of antioxidants (phenolic compounds).

Tables <NUM> to <NUM> show the extremely positive sensory evaluations for the gluten-free beers obtained with the method of the invention but also for baked goods made with the gluten-free spent grain flours according to the invention.

In the following description, the term "Beers*" refers to gluten-free beers produced with the method of the invention by applying the following conditions: addition of the flour mixture to the water at a temperature of <NUM>; protein rest: <NUM> for <NUM> minutes; re-fermentation in the bottle with the addition of sucrose (<NUM>/l).

By contrast, the term "Beers**" refers to gluten-free beers produced with the method of the invention under the following conditions: addition of the flour mixture to the water at a temperature of <NUM>; protein rest: <NUM> for <NUM> minutes; re-fermentation in the bottle with the addition of sucrose (<NUM>/l) and commercial strains of Saccharomyces cerevisiae yeast (approximately <NUM><NUM> cfu/ml).

Table <NUM> shows the physicochemical characteristics, gluten content, and total phenolic content of the beers produced with the method of the invention.

Table <NUM> shows the moisture content, gluten content, and total phenolic content of the gluten-free spent grains obtained with the method of the invention.

Table <NUM> shows the physicochemical characteristics, gluten content and total phenolic content of bread produced by replacing <NUM>% of commercial gluten-free flour with the gluten-free spent grain flours obtained with the method of the invention.

The letters "L", "a", "b", respectively, denote the colour coordinates according to the international standard CIE Lab: "L" is the lightness expressed as a percentage (%) (<NUM>% is white, <NUM>% is black); "a" and "b" are two colour ranges from red (positive values) to green (negative values) and from yellow (positive values) to blue (negative values), respectively.

Table <NUM> shows the physicochemical characteristics, gluten content, and total phenolic content of biscuits produced by replacing <NUM>% (biscuits <NUM>%) and <NUM>% (biscuits <NUM>%) of commercial gluten-free flour with the gluten-free spent grain flours obtained with the method of the invention.

The letters "L", "a", "b", respectively, denote the colour coordinates according to the international standard CIE Lab as previously defined with reference to Table <NUM>.

Table <NUM> shows the sensory characteristics of the gluten-free beers obtained with the method of the invention. During the evaluation, the following parameters were considered with the related rating scale:.

Table <NUM> shows the sensory characteristics of bread produced by replacing <NUM>% of commercial gluten-free flour with the gluten-free spent grain flours obtained with the method of the invention.

Table <NUM> shows the sensory characteristics of biscuits produced by replacing <NUM>% (biscuits <NUM>%) and <NUM>% (biscuits <NUM>%) of commercial gluten-free flour with the gluten-free spent grain flours obtained with the method of the invention.

Claim 1:
A method of producing gluten-free beer, wherein the method employs a mixture of a plurality of gluten-containing cereal grains as the starting material and comprises the steps of:
(i) contacting the mixture of a plurality of gluten-containing cereal grains with water at a temperature comprised between <NUM> and <NUM>, the grains being optionally in ground form;
(ii) heating the aqueous mixture of step (i) at a rate of about <NUM> per minute until a temperature of <NUM> is reached and keeping the aqueous mixture at said temperature for between <NUM> and <NUM> minutes;
(iii) further heating the aqueous mixture of step (ii) to a temperature comprised between <NUM> and <NUM> and keeping the aqueous mixture at said temperature for between <NUM> and <NUM> minutes, thereby obtaining a wort;
(iv) filtering the wort of step (iii) to separate the liquid fraction from the spent grains, thereby obtaining a clear wort;
(v) boiling the clear wort of step (iv) with hops at a temperature of <NUM> for between <NUM> and <NUM> minutes, thereby obtaining a hop-treated wort;
(vi) fermenting the hop-treated wort of step (v) with one or more yeasts belonging to the Saccharomyces genus at a temperature comprised between <NUM> and <NUM>, to obtain a gluten-free beer,
characterized in that the mixture of a plurality of gluten-containing cereal grains employed as the starting material comprises barley malt in an amount of between <NUM>% and <NUM>% by weight and one or more unmalted cereals in a total amount of between <NUM>% and <NUM>% by weight.