Patent Description:
Anorectic treatment with fenfluramine has been associated with the development of cardiac valvulopathy and pulmonary hypertension, including the condition cardiac fibrosis which led to the withdrawal of fenfluramine from world-wide markets. Interaction of fenfluramine's major metabolite norfenfluramine with the <NUM>-HT2B receptor is associated with heart valve hypertrophy. In the treatment of epilepsy, the known cardiovascular risks of fenfluramine are weighed against beneficial anticonvulsive activity. <CIT> describes compositions of fenfluramine and methods for their production.

The present disclosure provides methods of preparing a fenfluramine active pharmaceutical ingredient. Aspects of the subject methods include reacting a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition; and reductively aminating the <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition with ethylamine using a borohydride reducing agent to produce a fenfluramine composition. Also provided are fenfluramine compositions and pharmaceutical ingredients produced according to the subject methods that include a reduced amount of one or more minor components such as impurities or reaction side products. In some cases, the compositions include a pharmaceutically acceptable salt of fenfluramine having less than <NUM>% by weight in total of trifluoro methyl regioisomers. Also provided are pharmaceutical compositions including the subject fenfluramine compositions.

These and other objects, advantages, and features of the invention will become apparent to those persons skilled in the art upon reading the details of the metabolism-resistant fenfluramine analogs and methods of using the same as more fully described below.

The present disclosure is best understood from the following detailed description when read in conjunction with the accompanying drawings. Included in the drawings are the following figures.

As used herein, the term "subject" refers to a mammal. Exemplary mammals include, but are not limited to, humans, domestic animals (e.g., a dog, cat, or the like), farm animals (e.g., a cow, a sheep, a pig, a horse, or the like) or laboratory animals (e.g., a monkey, a rat, a mouse, a rabbit, a guinea pig, or the like). In certain embodiments, the subject is human. "Patient" refers to human and non-human subjects, especially mammalian subjects.

As used herein, the terms "treatment," "treating," and the like, refer to obtaining a desired pharmacologic and/or physiologic effect. The effect may be prophylactic in terms of completely or partially preventing a disease or symptom thereof and/or may be therapeutic in terms of a partial or complete cure for a disease and/or adverse effect attributable to the disease. As used herein, the terms "treating," "treatment," "therapeutic," or "therapy" do not necessarily mean total cure or abolition of the disease or condition. Any alleviation of any undesired signs or symptoms of a disease or condition, to any extent can be considered treatment and/or therapy. Furthermore, treatment may include acts that may worsen the patient's overall feeling of well-being or appearance. "Treatment," as used herein, covers any treatment of a disease in a mammal, in some cases in a human, and includes: (a) preventing the disease or medical condition from occurring, such as, prophylactic treatment of a subject; (b) ameliorating the disease or medical condition, such as, eliminating or causing regression of the disease or medical condition in a patient; (c) suppressing the disease or medical condition, for example by, slowing or arresting the development of the disease or medical condition in a patient; or (d) alleviating a symptom of the disease or medical condition in a patient.

As used herein, the term pKa refers to the negative logarithm (p) of the acid dissociation constant (Ka) of an acid, and is equal to the pH value at which equal concentrations of the acid and its conjugate base form are present in solution.

The term "salt" refers to an ionic compound that result from the neutralization reaction of an acid and a base, and is composed of at least one cation (positively charged ion) and at least one anion (negative ion). In some embodiments, a salt is electrically neutral (without a net charge). Where applicable, the salt is a pharmaceutically acceptable salt, although this is not required for salts of intermediate compounds that are not intended for administration to a patient. By way of example, salts of the present compounds include those wherein the basic compound is protonated by an inorganic or organic acid to form a conjugate acid cation, with the conjugate base of the inorganic or organic acid as the anionic component of the salt. Salts of interest include, but are not limited to, hydrochloride salts. It is understood that for any of the structures depicted herein, such structures may also include any convenient salt forms.

The term "pharmaceutically acceptable" means approved by a regulatory agency of the Federal or a state government or listed in the U. Pharmacopeia or other generally recognized pharmacopeia for use in mammals, such as humans.

The term "pharmaceutically acceptable salt" means a salt which is acceptable for administration to a patient, such as a mammal (salts with counterions having acceptable mammalian safety for a given dosage regime). Such salts can be derived from pharmaceutically acceptable inorganic or organic bases and from pharmaceutically acceptable inorganic or organic acids. "Pharmaceutically acceptable salt" refers to pharmaceutically acceptable salts of a compound, which salts are derived from a variety of organic and inorganic counter ions well known in the art and include, by way of example only, sodium, and the like; and when the molecule contains a basic functionality, salts of organic or inorganic acids, such as hydrochloride, and the like. Pharmaceutically acceptable salts of interest include, but are not limited to, hydrochloride salts.

The term "active pharmaceutical ingredient" (API) refers to a substance or mixture of substances intended to be used in the manufacture of a drug product and that, when used in the production of a drug, becomes an active ingredient in the drug product. Such substances are intended to furnish pharmacological activity or other direct effect in the diagnosis, cure, mitigation, treatment or prevention of disease or to affect the structure and function of the body.

"Solvate" refers to a complex formed by combination of solvent molecules with molecules or ions of the solute. The solvent can be an organic compound, an inorganic compound, or a mixture of both. Some examples of solvents include, but are not limited to, methanol, N,N-dimethylformamide, tetrahydrofuran, dimethylsulfoxide, and water. When the solvent is water, the solvate formed is a hydrate.

"Stereoisomer" and "stereoisomers" refer to compounds that have same atomic connectivity but different atomic arrangement in space. Stereoisomers include cis-trans isomers, E and Z isomers, enantiomers, and diastereomers.

"Tautomer" refers to alternate forms of a molecule that differ only in electronic bonding of atoms and/or in the position of a proton, such as enol-keto and imine-enamine tautomers, or the tautomeric forms of heteroaryl groups containing a -N=C(H)-NH- ring atom arrangement, such as pyrazoles, imidazoles, benzimidazoles, triazoles, and tetrazoles. A person of ordinary skill in the art would recognize that other tautomeric arrangements of the groups described herein are possible.

It will be appreciated that the term "or a salt or solvate or stereoisomer thereof" is intended to include all permutations of salts, solvates and stereoisomers, such as a solvate of a pharmaceutically acceptable salt of a stereoisomer of subject compound. It is understood that the term "or a salt thereof" is intended to include all permutations of salts. It is understood that the term "or a pharmaceutically acceptable salt thereof" is intended to include all permutations of salts. It is understood that the term "or a solvate thereof" is intended to include all permutations of solvates. It is understood that the term "or a stereoisomer thereof" is intended to include all permutations of stereoisomers. It is understood that the term "or a tautomer thereof" is intended to include all permutations of tautomers. Thus for example it follows that it is intended to include a solvate of a pharmaceutically acceptable salt of a tautomer of a stereoisomer of subject compound.

"Pharmaceutically effective amount" and "therapeutically effective amount" refer to an amount of a compound sufficient to treat a specified disorder or disease or one or more of its symptoms and/or to prevent the occurrence of the disease or disorder. In reference to tumorigenic proliferative disorders, a pharmaceutically or therapeutically effective amount comprises an amount sufficient to, among other things, cause the tumor to shrink or decrease the growth rate of the tumor.

The term "vehicle" refers to a diluent, adjuvant, excipient, or carrier with which a compound of the invention is formulated for administration to a mammal.

Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limits of that range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range, and each range where either, neither or both limits are included in the smaller ranges is also encompassed within the invention, subject to any specifically excluded limit in the stated range.

Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, some potential and preferred methods and materials are now described. It is understood that the present disclosure supercedes any disclosure of a referenced publication to the extent there is a contradiction.

It must be noted that as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to "a compound" includes a plurality of such compounds and reference to "the method" includes reference to one or more methods and equivalents thereof known to those skilled in the art, and so forth.

The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed.

Before the present compounds and methods are described, it is to be understood that this invention is not limited to particular compounds and methods described, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims.

As summarized above, the present disclosure provides methods of preparing a fenfluramine active pharmaceutical ingredient. Aspects of the present disclosure include fenfluramine compositions and pharmaceutical ingredients produced according to the subject methods where particular undesirable minor components of interest are substantially eliminated from the composition. The subject methods provide for a combination of steps to produce a crude composition that achieves a desirable minimum threshold for undesirable minor components, such as difficult to purify regioisomers, reaction side products and reagents. Active pharmaceutical ingredients for pharmaceutical formulations are prepared via controlled and reproducible methods to achieve highly pure compositions of active agent which provide high levels of safety, efficacy and quality in the resulting pharmaceutical formulations. In some cases, impurities or undesirable minor components in pharmaceutical compositions can cause drug product instability, loss of potency and toxicity. The substantial elimination of such minor components from the subject fenfluramine compositions provides a composition that is suitable for use in pharmaceutical compositions as the active pharmaceutical ingredient (API). The subject compositions can be produced efficiently with a reduced need for purification, eliminating purification steps or improving the outcome of method steps such as those steps involving removal of difficult to remove regioisomers of fenfluramine.

The term "composition", when used in the context of the subject methods, describes a material that is a starting material or a product of one or more steps of the subject methods and which can contain a mixture of components. The composition can be referred to by its predominant or target component, e.g., a fenfluramine composition. In general terms, a composition can include, in addition to a predominant target component, a mixture of other components, such as target isomers (e.g., a stereoisomer or regioisomer), impurities, reaction side products, starting materials, carry-over components from previous steps, reagents, solvents, and the like. As used herein, the term "crude composition" refers to the material produced in the performance of a chemical reaction procedure which has not been subjected to additional purification steps, e.g., separate post-reaction procedure steps, such as chromatography or recrystallization steps. In the preparation of a crude composition, the material can be subjected to simple steps, e.g., such as aqueous washes, solvent extractions and/or filtrations, which are considered an integral part of the reaction procedure, because such steps are commonly used to terminate a chemical reaction and/or to "work-up" a reaction product. Such reaction workup steps are not considered to be additional purification steps, as described above, but are merely part of the preparation of a crude composition.

Aspects of the subject methods include preparation of a fenfluramine composition from a <NUM>-(<NUM>-(trifluoromethyl)phenyl)-propan-<NUM>-one precursor composition via reductive amination (Scheme <NUM>).

Any convenient methods of reductive amination may be utilized to convert the ketone (<NUM>) to fenfluramine (<NUM>) via an imine intermediate (1a), e.g., via a Schiff base formed between ethylamine (e.g., Et-NH<NUM>) and the ketone (<NUM>). Methods and reagents of interest include, but are not limited to, those methods and reagents described by <NPL>. In some embodiments, the reductive amination reaction is performed under conditions that comprise contacting the <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition with a solution of <NUM>% by weight of ethylamine/water and about <NUM> equivalents or more of sodium triacetoxyborohydride dissolved in methanol as solvent. In certain cases, the reaction (e.g., scheme <NUM>) is performed at an industrial scale (e.g., as described herein). In certain instances, the yield of the reaction (e.g., scheme <NUM>) is <NUM>% or more, such as <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more or <NUM>% or more.

Any convenient reducing agents can be used in the reductive amination step of the subject methods, e.g., to reduce the Schiff base intermediate to the secondary amine product, fenfluramine. In some instances, the reducing agent is a borohydride reducing agent. As used herein, the term "borohydride reducing agent" is meant to include any reducing agent that includes a BH- group, such as any convenient borohydride, cyanoborohydride or triacetoxyborohydride reducing agent having the formula MBR<NUM>H, where each R is independently H, alkyl, cyano or acetoxy and M is a metal such as Na, Li or K. In some instances, the reducing agent is a cyanoborohydride reducing agent. In some instances, the reducing agent is a triacetoxyborohydride reducing agent. In some cases, the reducing agent is selected from sodium borohydride, sodium cyanoborohydride, sodium triacetoxyborohydride, lithium triethylborohydride, nickel borohydride, potassium borohydride and calcium borohydride. In certain instances the borohydride reducing agent is sodium triacetoxyborohydride (STAB; Na(CH<NUM>COO)<NUM>BH).

The <NUM>-(<NUM>-(trifluoromethyl)phenyl)-propan-<NUM>-one (<NUM>) composition can be prepared from any convenient precursor composition. In some instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)-propan-<NUM>-one (<NUM>) composition is prepared from <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>), e.g., according to Scheme <NUM> via a Daikin-West reaction. As such, aspects of the subject methods include reacting the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition.

The Daikin-west reaction provides for the conversion of an enolizable carboxylic acid to a corresponding methyl ketone by reaction with an acetylation agent (e.g., acetic anhydride and a catalyst). In some cases, the catalyst is a nucleophilic catalyst. Any convenient nucleophilic catalyst can be used in junction with acetic anhydride in the preparation of ketone (<NUM>) via Scheme <NUM>. In some embodiments, the catalyst is N-methylimidazole (i.e., <NUM>-methylimidazole). The catalyst and the acetic anhydride may combine to form an acetylating agent in situ. It is understood that a variety of other acetylating agents and precursor reagents for producing an acetylation agent in situ may be utilized in the reaction step. In some cases, the method step includes addition of a preformed acetylating agent directly to the acid (<NUM>). Methods and reagents of interest that find use in the preparation of ketone (<NUM>) include, but are not limited to, those described by <NPL>. In some embodiments, the reaction is performed under conditions that include contacting the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition with about <NUM> equivalents of <NUM>-methylimidazole and about <NUM> equivalents or more of acetic anhydride, optionally in a solvent. In certain instances, the yield of the reaction (e.g., scheme <NUM>) is <NUM>% or more, such as <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more or <NUM>% or more.

The ketone (<NUM>) can be optionally purified before use in the step outlined in Scheme <NUM> using any convenient method. In some cases, the ketone (<NUM>) is purified via formation of a bisulfite adduct. As used here, the terms "bisulfite adduct" and "bisulfite addition compound" are used interchangeably to refer to the product of addition of a bisulfite ion to a ketone compound. The bisulfite adduct of ketone (<NUM>) can be a solid which provides for a more facile removal of impurities from the adduct composition than is possible from the corresponding parent ketone composition.

Aspects of the subject methods include a combination of the individual steps described herein, e.g., a combination of steps as step forth in Scheme <NUM>. Before or after any of the steps described an optional additional purification step (e.g., crystallization step) may be performed. In some embodiments, the method includes reacting a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition; and reductively aminating the <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition with ethylamine using a borohydride reducing agent to produce a fenfluramine composition.

The <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>) composition can be prepared from any convenient precursor composition. In some instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition is prepared from a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition, e.g., according to the reaction of Scheme <NUM>. As such, aspects of the subject method includes hydrolyzing a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile (<NUM>) composition to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>) composition.

Hydrolysis of the nitrile (<NUM>) to the acid (<NUM>) can be achieved using any convenient methods. In some cases, the hydrolysis of the nitrile (<NUM>) is achieved via acid-catalyzed hydrolysis. In certain instances, the hydrolysis of the nitrile (<NUM>) is achieved via base-catalyzed hydrolysis. Hydrolysis may proceed via an amide intermediate (4a) under aqueous acidic conditions. In some embodiments of the method, hydrolysis of the nitrile (<NUM>) to the acid (<NUM>) is performed under aqueous acidic conditions. In certain instances, the yield of the reaction (e.g., scheme <NUM>) is <NUM>% or more, such as <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more or <NUM>% or more.

In some cases, the method includes hydrolyzing a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile (<NUM>) composition to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>) composition; and reacting a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>) composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one (<NUM>) composition (see e.g., Scheme <NUM>).

Aspects of the subject methods include a combination of the steps described herein e.g., a combination of steps as described in Scheme <NUM>. Before or after any of the steps described, an optional additional purification step (e.g., crystallization step) may be performed. In some embodiments, the method includes hydrolyzing a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile (<NUM>) composition to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>) composition; reacting the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid (<NUM>) composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one (<NUM>) composition; and reductively aminating the <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one (<NUM>) composition with ethylamine using a borohydride reducing agent to produce a fenfluramine (<NUM>) composition.

In some embodiments of the method, the fenfluramine composition (e.g., a crude fenfluramine composition) that is produced has the following profile: <NUM>% or more by weight of fenfluramine or a salt thereof, such as <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, or even more by weight of the fenfluramine or salt thereof; <NUM>% or less by weight of <NUM>-fenfluramine regioisomer or a salt thereof, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less by weight of <NUM>-fenfluramine regioisomer or a salt thereof; <NUM>% or less by weight of <NUM>-fenfluramine regioisomer or a salt thereof, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less by weight of <NUM>-fenfluramine regioisomer or a salt thereof; and <NUM>% or less by weight of fenfluramine reduced alcohol side product, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM>% or less by weight of fenfluramine reduced alcohol side product.

In some embodiments, the method is a method of preparing fenfluramine free base. As such, the fenfluramine composition can include fenfluramine free base. Fenfluramine free base that is prepared according to the subject methods may be converted to any convenient salt form, e.g., a salt of the conjugate acid of the secondary amino group of fenfluramine (fenfluramine. H+X-), using a variety of methods. The formation of a fenfluramine salt can be performed as part of the reductive amination step of Scheme <NUM> (e.g., in situ), or salt formation can be performed in an optional subsequent step. In some cases, the salt form is a pharmaceutically acceptable salt of fenfluramine. Salts of interest include, but are not limited to, a hydrochloride salt. In certain instances, the pharmaceutically acceptable salt form of fenfluramine is a hydrochloride salt.

The subject methods provide for substantial elimination of one or more undesirable minor components from the crude fenfluramine composition or fenfluramine salt composition, such that final additional purification steps can be achieved easily with high efficiency and/or high yield to produce a high quality active pharmaceutical composition.

One or more additional purification steps may be performed on the crude fenfluramine composition (e.g., that includes a free base or a salt form of fenfluramine) prepared according to the subject methods. In certain instances, the purification step includes crystallization of fenfluramine or the salt form of fenfluramine from the crude composition. The crystalline fenfluramine salt form can have a desirable polymorphism, high crystallinity, water solubility and/or stability. In some cases, the subject methods provide for a crystalline fenfluramine hydrochloride salt that is a single polymorph that is free-flowing, non-hygroscopic and having a high melting temperature.

In some embodiments of the method, the composition produced comprises a pharmaceutically acceptable salt of fenfluramine and has following purity profile: <NUM>% or more of the pharmaceutically acceptable salt of fenfluramine, such as <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, <NUM>% or more, or even more by weight of the pharmaceutically acceptable salt of fenfluramine; <NUM>% or less by weight of <NUM>-fenfluramine; <NUM>% or less by weight of <NUM>-fenfluramine regioisomer or a salt thereof, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less by weight of <NUM>-fenfluramine regioisomer or a salt thereof; <NUM>% or less by weight of <NUM>-fenfluramine regioisomer or a salt thereof, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less by weight of <NUM>-fenfluramine regioisomer or a salt thereof; and <NUM>% or less by weight of fenfluramine reduced alcohol side product, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM> % or less by weight of fenfluramine reduced alcohol side product. In certain embodiments, the composition produced according to the subject methods is a fenfluramine active pharmaceutical ingredient comprising a pharmaceutically acceptable salt of fenfluramine and having <NUM>% or less by weight in total of trifluoromethyl regioisomers, such as <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less by weight of trifluoromethyl regioisomers. In certain embodiments, the fenfluramine active pharmaceutical ingredient has a purity profile comprising: at least <NUM>% (e.g., at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, or more) by weight of a pharmaceutically acceptable salt of fenfluramine; less than <NUM>% by weight (e.g., less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% by weight) of <NUM>-fenfluramine; less than <NUM>% by weight (e.g., less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% by weight) of <NUM>-fenfluramine; and less than <NUM>% by weight (e.g., less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% by weight) of fenfluramine alcohol.

The subject methods provide for the preparation of a racemic mixture of enantiomers of fenfluramine. The enantiomers of fenfluramine may be referred to as: dexfenfluramine (i.e., (S)-N-ethyl-<NUM>-[<NUM>-(trifluoromethyl)phenyl]-propan-<NUM>-amine, (+)-fenfluramine or (S)-fenfluramine); and levofenfluramine (i.e., (2R)-N-ethyl-<NUM>-[<NUM>-(trifluoromethyl)phenyl]-<NUM>-propanamine, (-)-fenfluramine or (R)-fenfluramine). The fenfluramine enantiomers or salts thereof can be separated from each other using any convenient methods. Methods of interest for separation and purification of fenfluramine enantiomers include, but are not limited to, chiral resolution by crystallization and chiral column chromatography. As such, in some embodiments, the method further includes performing a chiral separation of a racemic fenfluramine composition, or a salt thereof, to produce a non-racemic fenfluramine composition comprising a predominant stereoisomer of fenfluramine. By non-racemic is meant a composition having an enantiomeric excess of at least <NUM>%, such as at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>% or at least <NUM>% of one stereoisomer, e.g., a predominant stereoisomer. As used herein, the term "predominant stereoisomer" is meant to encompass a composition including only one stereoisomer or a composition that includes stereoisomer mixtures.

In some cases, the active pharmaceutical ingredient composition is a non-racemic composition including (S)-fenfluramine or a pharmaceutically acceptable salt thereof as the predominant stereoisomer. In some cases, the active pharmaceutical ingredient composition is a non-racemic composition including (R)-fenfluramine or a pharmaceutically acceptable salt thereof as the predominant stereoisomer. In some cases, the non-racemic composition that is produced includes only one stereoisomer.

As summarized above, the compositions of the subject methods, e.g., starting material compositions, intermediate compositions and final fenfluramine compositions may provide for the substantial elimination of one or more minor components, which is achieved by the subject methods to produce compositions that find use as an active pharmaceutical ingredient (API), or precursor thereof, for pharmaceutical compositions. The subject methods provide for the substantial elimination of undesirable minor components is a variety of ways. As used herein, by "substantially eliminate" is meant the achievement of a desirable minimum threshold for a minor component of interest, such that the minor component, if present, is present at a level at or below the threshold. As used herein, the term "substantially devoid" refers to a composition where a minor component of interest is either not present or is present at a level at or below the minimum threshold. The desirable minimum threshold for a minor component of interest may vary according to the nature of the component and whether the composition in an intermediate composition or a fenfluramine composition of interest. In some instances, the desirable minimum threshold of a minor component of interest that is achieved is <NUM>% by weight or less, such as <NUM>% or less, <NUM>% or less, or <NUM>% or less, <NUM>% or less, <NUM>% or less <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less,<NUM>% or less, or <NUM>% or less. In certain instances, the minor component of interest is completely eliminated from the compositions of interest, i.e., the composition is devoid of the minor component (e.g., is not detected or is below the detectable limit of the component).

In some cases, the particular combination of steps utilized in the subject method works to eliminate a minor component of interest. In certain instances, purification of an intermediate composition, e.g., via crystallization, achieves substantial elimination of a minor component that would be difficult to remove if the minor component, e.g., a regioisomer, was carried forward to a later step in the synthesis. In certain instances, the performance of a particular method step provides for a selectivity of reaction, whereby a minor component of interest is not transformed by the reaction conditions as the major component is, and thus may be more easily removed, e.g., as a regioisomer of the starting material rather than the product of a reaction or particular method step. In some cases, the particular combination of steps utilized in the subject methods avoids the use of one or more chemical reagents, solvents and/or reactants that is required via conventional methods and which are lead to undesirable minor components in the product compositions. Minor components of interest which may be substantially eliminated include but are not limited to, product isomers, side products, aldehydes, ketones, peroxides, metals (e.g., heavy metal and metal catalysts), nitrate/nitrite, trace solvents, and organic acids. Various minor components and details of their substantial elimination from the subject compositions are now described in greater below. Minor components of interest that may be substantially eliminated according to the subject methods include any impurities, by-products, starting materials and minor components described herein, including but not limited to, acetate impurity, dimer impurity, Acetamide impurity, <NUM>-((<NUM>-trifluoromethyl)phenyl)acetone, fenfluramine regioisomers, Fenfluramine Alcohol, N-(<NUM>-(trifluoromethyl)-benzyl)ethanamine, norfenfluramine and any one of the impurities of Table <NUM>.

In some instances, a regioisomer of fenfluramine, or a precursor thereof, can be present as a minor component of any one of the subject compositions that find use in the subject methods. Fenfluramine and synthetic precursors thereof can include a <NUM>-trifluoromethyl substituted phenyl group. As used here, the terms , "trifluoromethyl regioisomer" and "trifluoromethyl-phenyl regioisomer" are used interchangeably to refer to an isomer(s) of fenfluramine, or any one of the synthetic precursors described herein, where the trifluoromethyl substituent is located at either the <NUM>-position or the <NUM>-position of the substituted phenyl ring rather than at the <NUM>-position corresponding to fenfluramine. As such, the terms "<NUM>-trifluoromethyl regioisomer" and "<NUM>-trifluoromethyl regioisomer" can be used herein to describe particular minor components of any intermediate composition or final composition that finds use in the subject methods.

The <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition starting material of the subject methods can include regioisomers. In some cases, the regioisomers derive from the method of preparation of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile from trifluoromethyl benzene. In some instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition comprises at least <NUM>% by weight, such as at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, or even more by weight of trifluoromethyl-phenyl regioisomers (e.g., a combined total of <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile and <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile). In some instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition comprises at least <NUM>% by weight, such as at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, or even more by weight of <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile). In some instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition comprises at least <NUM>% by weight, such as at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, or even more by weight of <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile). In certain instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition includes minor regioisomer components that are carried over to the next composition, e.g., a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition. As such, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition produced as an intermediate in the subject method can also include regioisomers (e.g., <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid and <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid) at the same levels as are described herein for the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition starting material.

The subject methods provide for removal of <NUM>- and/or <NUM>-regioisomers as minor components of an intermediate composition in various ways. In some embodiments, the method includes purifying the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition to produce a composition substantially devoid of one or both of the trifluoromethyl-phenyl regioisomers. In certain instances, the composition is also substantially devoid of benzaldehyde that is present in the acetonitrile starting material. In certain instances, the composition is also substantially devoid of trifluoromethyl-benzaldehyde that is present in the acetonitrile starting material. In certain instances, purifying the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition to remove a portion or all of the minor regioisomer components can be achieved via crystallization of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid. As used herein, the term "substantially devoid of a trifluoromethyl-phenyl regioisomer" means less than <NUM>% by weight, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, or even less. Any convenient methods of crystallization or recrystallization can be utilized in the subject methods.

After purification, e.g., crystallization, of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition, the composition can include less than <NUM>% by weight, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, or even less of <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid. After purification, e.g., crystallization, of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition, the composition can include less than <NUM>% by weight, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, or even less of <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid. After purification, e.g., crystallization, of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition, the composition can include less than <NUM>% by weight, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, or even less of benzaldehyde.

In some embodiments, the method includes reacting the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition, where the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid is selectively converted to the ketone in the presence of unreacted <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid. The subject method provides for facile removal of <NUM>-regioisomer that is present because this regioisomer is not carried through the reaction at the same rate as the target <NUM>-trifluoromethyl compound. In some cases, the method further comprises removing unreacted <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid regioisomer from the <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition. As such, in some instances, the crude <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition is substantially devoid (e.g., includes less than <NUM>% by weight, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, or even less) of <NUM>-regioisomer of the ketone product.

Removal of regioisomer minor components present in the acetonitrile starting material may be achieved in stages during performance of the synthetic method. In some embodiments, a first portion of the regioisomer minor components present in the starting material are removed from the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition, e.g., via crystallization. In certain instance, a second portion of the regioisomer minor components present that are carried through intermediate compositions of the subject method are removed via selective reaction of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid, e.g., as described herein. In certain instances, a third portion of the regioisomer minor components present that are carried through intermediate compositions of the subject method are removed via purification of a fenfluramine composition.

Depending on the method of preparation of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile, the starting material composition can include benzaldehyde or trifluorobenzaldehyde as a minor component. It is undesirable to have such a minor component present in a pharmaceutical active ingredient. In some instances, the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetonitrile composition comprises at least <NUM>% by weight, such as at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, at least <NUM>%, or even more by weight of benzaldehyde or trifluorobenzaldehyde as a minor component. In some instances, any benzaldehyde or trifluorobenzaldehyde that is present as a minor component is substantially removed during purification, e.g., crystallization, of the <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid from the composition as described herein. In certain instances, benzaldehyde is not present in the <NUM>-(<NUM>-(trifluoromethyl)phenyl)-acetonitrile starting material composition due to its method of preparation.

The subject methods can include a particular combination of steps for preparation of the ketone (<NUM>) that provide for one or more advantages over other possible methods. <FIG> illustrates a variety of synthetic pathways that could be used for preparation of ketone (<NUM>). In certain cases, the particular method that finds use in the subject methods is preparation of ketone (<NUM>) from nitrile (<NUM>) via acid (<NUM>).

In the subject methods, minor components (e.g., acetate and dimer impurities) formed during the Dakin-West reaction (e.g., as described in Scheme <NUM>) can be subsequently substantially eliminated. In certain cases, these minor components are removed using a distillation procedure. In certain instances, these minor components are removed via a procedure including isolation of the product ketone (<NUM>) as the bisulfite salt (e.g., as described herein). The acetate and dimer impurities are shown below. <CHM>
In some instances, use of the bisulfite isolation procedure improves the purity of the ketone by a factor of at least <NUM>% (e.g., at least <NUM>%, at least <NUM>%, or more) by removing these and other impurities. In some embodiments, the subject methods provide for substantial elimination of the acetate impurity from the ketone (<NUM>) composition. In some embodiments, the subject methods provide for substantial elimination of the dimer impurity from the ketone (<NUM>) composition.

<FIG> illustrates a diazonium route to prepare ketone (<NUM>) from an Aryl Nitro starting material. The diazonium route has a disadvantage due to the potential formation of genotoxic intermediates shown as boxed compounds (e.g., N-hydroxyaryl, N-nitrosamine and Nitro compound). In some cases, removal of such impurities and/or demonstrating their absence is costly and time consuming and sometimes difficult to achieve technically. Aspects of the subject methods include a synthetic route that substantially eliminates the undesirable minor components that are possible via the route shown in <FIG>, thereby circumventing the potential for such toxic and/or undesirable compounds to be present in the subject compositions.

In some cases, the subject methods provide for elimination of isomer (e.g., a regioisomer) by-products of the <NUM>-trifluoroaniline starting material described in <FIG>. Such by-products can be present in <NUM>-trifluoroaniline compositions, carried through synthetic steps, and be difficult to substantially eliminate from downstream compositions. In some instances of the subject methods, crystallization of the Acid (<NUM>) resulting from hydrolysis of the nitrile (<NUM>) provides crystalline Acid (<NUM>) which provides for a facile removal of such isomers early in synthesis. Removing impurities and/or undesirable isomers early in a synthesis can be preferred, especially if such impurities are carried through to final product compositions, as purification of a final product at the end of a synthesis is more costly (e.g., in losses of valuable product) and impacts cost of goods more greatly than removing such minor components early in synthesis before raw materials are invested along the process.

The subject methods include a particular synthetic pathway and combination of chemical reactions (e.g., as described above) that provides for the elimination of certain undesirable reagents and/or solvents (e.g., Class <NUM> or Class <NUM> solvents that have known or they have strongly suspected carcinogenic activity and/or are environmental hazards). Class <NUM> and <NUM> solvents of interest which can be eliminated from the fenfluramine composition by practicing the subject methods include, but are not limited to, any solvent listed on the International Conference on Harmonization (ICH) Q3C list and guidance for Industry (February <NUM>, Revision <NUM>, US Dept. HHS), such as acetonitrile, benzene and substituted benzenes, carbon tetrachloride, chloroform, cyclohexane, <NUM>,<NUM>-dichloroethane, <NUM>,<NUM>-dichloroethane, <NUM>,<NUM>-dimethoxyethane, DMF, <NUM>,<NUM>-dioxane, methanol, methylbutyl ketone, N-methylpyrrolidinone, pyridine, toluene, <NUM>,<NUM>,<NUM>-trichloroethane, <NUM>,<NUM>,<NUM>-trichloroethene, and xylene. The subject methods also provide for the elimination of a variety of undesirable and/or toxic reagents from the fenfluramine composition that is produced by practicing the subject methods. For example, by including a reductive amination step according to the method depicted in Scheme <NUM>, alternative synthetic pathways that require use of potentially toxic metal catalysts are avoided. By eliminating the use of such reagents and/or solvents from the synthetic pathway of the subject methods, potentially toxic minor components are eliminated from the fenfluramine composition. As such, the subject fenfluramine composition can be referred to as being substantially devoid of the minor component of interest. In some instances, one or more potential heavy metal components such as Pb, As, Cd, Hg, Pb, Co, Mo, Se and V are substantially eliminated. In certain instances, one or more Class <NUM> solvents are substantially eliminated (e.g., below an acceptable threshold limit as adopted under ICH Q3C). In certain instances, benzene solvent is substantially eliminated, e.g., below a concentration limit of <NUM> ppm. In certain instances, carbon tetrachloride solvent is substantially eliminated, e.g., below a concentration limit of <NUM> ppm. In certain instances, <NUM>,<NUM>-dichloroethane solvent is substantially eliminated, e.g., below a concentration limit of <NUM> ppm. In certain instances, <NUM>,<NUM>-dichloroethane solvent is substantially eliminated, e.g., below a concentration limit of <NUM> ppm. In certain instances, <NUM>,<NUM>,<NUM>-trichloroethane solvent is substantially eliminated, e.g., below a concentration limit of <NUM> ppm. A minor component can be considered completely eliminated from the subject compositions when the fenfluramine is produced via a method where the minor component is not used in any synthetic step or present in a starting material.

As used herein, the terms "fenfluramine alcohol" and "reduced alcohol side product" are used interchangeable to refer to the product of ketone reduction to alcohol that can occur in the reductive amination step of Scheme <NUM>, depicted below.

The subject methods provide for substantial elimination of fenfluramine alcohol from the subject compositions. In some instances, the crude fenfluramine composition has less than <NUM>% by weight of the reduced alcohol side product, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% , less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% or even less. In some instances, the crude fenfluramine composition has <NUM>% or less by weight of the reduced alcohol side product, such as <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less.

Norfenfluramine is a potential impurity of compositions that include fenfluramine. The subject methods provide for substantial elimination of norfenfluramine from the subject compositions. In some instances, the crude fenfluramine composition has less than <NUM>% by weight of norfenfluramine, such as less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% , less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>%, less than <NUM>% or even less. In some instances, the crude fenfluramine composition includes has <NUM>% or less by weight of norfenfluramine, such as <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, <NUM>% or less, or even less.

Fenfluramine and the fenfluramine compositions described herein may be employed in a variety of methods. Aspects of the present disclosure include a method that includes administering to a subject in need thereof a therapeutically effective amount of a fenfluramine pharmaceutical composition (e.g., as described herein) to treat or prevent a disease or condition of interest. By "therapeutically effective amount" is meant the concentration of a compound that is sufficient to elicit the desired biological effect (e.g., treatment or prevention of epilepsy). Diseases and conditions of interest include, but are not limited to, epilepsy, a neurological related diseases, obesity and obesity related diseases.

In some embodiments, the subject method includes administering to a subject a subject composition to treat a neurological related disease. Neurological related diseases of interest include, but are not limited to, epilepsy, and Dravet syndrome. In certain embodiments, the subject is human. In certain instances, the subject suffers from Dravet syndrome. In certain embodiments, the compound is administered as a pharmaceutical preparation.

Thus, according to a still further aspect of the present disclosure, there is provided a method of stimulating one or more <NUM>-HT receptors in the brain of a patient by administering an effective dose of a fenfluramine composition to said patient, said one or more <NUM>-HT receptors being selected from one or more of <NUM>-HT<NUM>, <NUM>-HT1A, <NUM>-HT1B, <NUM>-HT1C, <NUM>-HT1D, <NUM>-HT1E, <NUM>-HT1F, <NUM>-HT<NUM>, <NUM>-HT2A, <NUM>-HT2B, <NUM>-HT2C, <NUM>-HT<NUM>, <NUM>-HT<NUM>, <NUM>-HT<NUM>, <NUM>-HT5A, <NUM>-HT5B, <NUM>-HT<NUM>, and <NUM>-HT<NUM> amongst others. In some instances, the <NUM>-HT receptor is <NUM>-HT2B. In certain embodiments of this aspect of the invention, the patient has been diagnosed with Dravet Syndrome. In some instances, the method is a method of treating Dravet Syndrome that includes of stimulating one or more <NUM>-HT receptors in the brain of a patient by administering an effective dose of a fenfluramine composition to said patient, said one or more <NUM>-HT receptors being selected from one or more of <NUM>-HT1D, <NUM>-HT2A and <NUM>-HT2C, among others.

There are a number of genetic mutations that are indicative of Dravet Syndrome. Mutations in the SCNlA (such as partial or total deletion mutations, truncating mutations and/or missense mutations e.g. in the voltage or pore regions S4 to S6), SCNl B (such as the region encoding the sodium channel β1 subunit), SCN2A, SCN3A, SCN8A, SCN9A, GABRG2 (such as the region encoding the γ2 subunit), GABRD (such as the region encoding the δ subunit) and/or PCDH19 genes have been linked to Dravet Syndrome.

Thus, according to a further aspect of the present invention, there is provided a method of treating a patient that exhibits a mutation in one, some or all of the above genes by administering to that patient an effective dose of a fenfluramine comp. In certain embodiments of this aspect of the invention, the patient has been diagnosed with Dravet Syndrome.

In embodiments of the invention, any effective dose of the fenfluramine composition can be employed. However, surprisingly low doses of fenfluramine compositions are found by the inventors to be efficacious, particularly for inhibiting or eliminating seizures in epilepsy patients. Thus, in some cases, in a preferred embodiment of the invention, a daily dose of less than about <NUM>/kg/day such as, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, less than about <NUM>/kg/day, such as about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day, about <NUM>/kg/day or about <NUM>/kg/day to about <NUM>/kg/day, about <NUM>/kg/day, or about <NUM>/kg/day is employed. Put differently, a preferred dose is less than about <NUM>/kg/day to about <NUM>/kg/day. In some cases, the dose is less than about <NUM>/kg/day to about <NUM>/kg/day, such as less than about <NUM>/kg/day to about <NUM>, mg/kg/day, less than about <NUM>/kg/day to about <NUM>/kg/day, less than about <NUM>/kg/day to about <NUM>/kg/day, less than about <NUM>/kg/day to about <NUM>/kg/day, or less than about <NUM>/kg/day to about <NUM>/kg/day.

As indicated above the dosing is based on the weight of the patient. However, for convenience the dosing amounts may be preset such as in the amount of <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM>. In certain instances, the dosing amount may be preset such as in the amount of about <NUM> to about <NUM>, such as about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, or about <NUM>. The dosing amounts described herein may be administered one or more times daily to provide for a daily dosing amount, such as once daily, twice daily, three times daily, or four or more times daily, etc. In certain embodiments, the dosing amount is a daily dose of <NUM> or less, such as <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, about <NUM>, or about <NUM>. In general the smallest dose which is effective should be used for the particular patient. In some cases, the dose is generally well below the dosing used in weight loss.

Administration of the subject pharmaceutical compositions may be systemic or local. In certain embodiments, administration to a mammal will result in systemic release of fenfluramine (for example, into the bloodstream). Methods of administration may include enteral routes, such as oral, buccal, sublingual, and rectal; topical administration, such as transdermal and intradermal; and parenteral administration. Suitable parenteral routes include injection via a hypodermic needle or catheter, for example, intravenous, intramuscular, subcutaneous, intradermal, intraperitoneal, intraarterial, intraventricular, intrathecal, and intracameral injection and non-injection routes, such as intravaginal rectal, or nasal administration. In certain embodiments, the compositions of the present disclosure are administered orally. In certain embodiments, it may be desirable to administer one or more compounds of the invention locally to the area in need of treatment. This may be achieved, for example, by local infusion during, topical application, by injection, by means of a catheter, by means of a suppository, or by means of an implant, said implant being of a porous, non-porous, or gelatinous material, including membranes, such as sialastic membranes, or fibers.

The dose of fenfluramine administered in the methods of the present invention can be formulated in any pharmaceutically acceptable dosage form including, but not limited to oral dosage forms such as tablets including orally disintegrating tablets, capsules, lozenges, oral solutions or syrups, oral emulsions, oral gels, oral films, buccal liquids, powder e.g. for suspension, and the like; injectable dosage forms; transdermal dosage forms such as transdermal patches, ointments, creams; inhaled dosage forms; and/or nasally, rectally, vaginally administered dosage forms. Such dosage forms can be formulated for once a day administration, or for multiple daily administrations (e.g. <NUM>, <NUM> or <NUM> times a day administration).

In some embodiments, the subject method includes administering to a subject an appetite suppressing amount of the subject compound to treat obesity. Any of the methods of administration and dosage forms of the subject compositions may be utilized in treating obesity.

Combination therapy includes administration of a single pharmaceutical dosage formulation which contains the subject composition and one or more additional agents; as well as administration of the subject composition and one or more additional agent(s) in its own separate pharmaceutical dosage formulation. For example, a subject composition and an additional agent active with appetite suppressing activity (e.g., phentermine or topiramate) can be administered to the patient together in a single dosage composition such as a combined formulation, or each agent can be administered in a separate dosage formulation. Where separate dosage formulations are used, the subject composition and one or more additional agents can be administered concurrently, or at separately staggered times, e.g., sequentially.

In some embodiments, the subject method is an in vitro method that includes contacting a sample with a subject composition. The protocols that may be employed in these methods are numerous, and include but are not limited to, serotonin release assays from neuronal cells, cell-free assays, binding assays (e.g., 5HT2B receptor binding assays); cellular assays in which a cellular phenotype is measured, e.g., gene expression assays; and assays that involve a particular animal model for a condition of interest (e.g., Dravet syndrome).

Also provided are pharmaceutical preparations that include fenfluramine active pharmaceutical ingredient compositions prepared according to the subject methods. Pharmaceutical preparations are compositions that include a compound (either alone or in the presence of one or more additional active agents) present in a pharmaceutically acceptable vehicle. In some embodiments, the pharmaceutical composition includes a fenfluramine composition (e.g., as described herein) formulated in a pharmaceutically acceptable excipient.

The choice of excipient will be determined in part by the particular compound, as well as by the particular method used to administer the composition. Accordingly, there is a wide variety of suitable formulations of the pharmaceutical composition of the present invention.

The dosage form of fenfluramine employed in the methods of the present invention can be prepared by combining the fenfluramine composition with one or more pharmaceutically acceptable diluents, carriers, adjuvants, and the like in a manner known to those skilled in the art of pharmaceutical formulation.

The subject compositions can be formulated into preparations for injection by dissolving, suspending or emulsifying them in an aqueous or nonaqueous solvent, such as vegetable or other similar oils, synthetic aliphatic acid glycerides, esters of higher aliphatic acids or propylene glycol; and if desired, with conventional additives such as solubilizers, isotonic agents, suspending agents, emulsifying agents, stabilizers and preservatives.

In some embodiments, formulations suitable for oral administration can include (a) liquid solutions, such as an effective amount of the compound dissolved in diluents, such as water, or saline; (b) capsules, sachets or tablets, each containing a predetermined amount of the active ingredient (fenfluramine), as solids or granules; (c) suspensions in an appropriate liquid; and (d) suitable emulsions. Tablet forms can include one or more of lactose, mannitol, corn starch, potato starch, microcrystalline cellulose, acacia, gelatin, colloidal silicon dioxide, croscarmellose sodium, talc, magnesium stearate, stearic acid, and other excipients, colorants, diluents, buffering agents, moistening agents, preservatives, flavoring agents, and pharmacologically compatible excipients. Lozenge forms can include the active ingredient in a flavor, usually sucrose and acacia or tragacanth, as well as pastilles including the active ingredient in an inert base, such as gelatin and glycerin, or sucrose and acacia, emulsions, gels, and the like containing, in addition to the active ingredient, such excipients as are described herein.

The subject formulations can be made into aerosol formulations to be administered via inhalation. These aerosol formulations can be placed into pressurized acceptable propellants, such as dichlorodifluoromethane, propane, nitrogen, and the like. They may also be formulated as pharmaceuticals for non-pressured preparations such as for use in a nebulizer or an atomizer.

In some embodiments, formulations suitable for parenteral administration include aqueous and non-aqueous, isotonic sterile injection solutions, which can contain anti-oxidants, buffers, bacteriostats, and solutes that render the formulation isotonic with the blood of the intended recipient, and aqueous and non-aqueous sterile suspensions that can include suspending agents, solubilizers, thickening agents, stabilizers, and preservatives. The formulations can be presented in unit-dose or multi-dose sealed containers, such as ampules and vials, and can be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid excipient, for example, water, for injections, immediately prior to use. Extemporaneous injection solutions and suspensions can be prepared from sterile powders, granules, and tablets of the kind previously described.

Formulations suitable for topical administration may be presented as creams, gels, pastes, or foams, containing, in addition to the active ingredient, such carriers as are appropriate. In some embodiments the topical formulation contains one or more components selected from a structuring agent, a thickener or gelling agent, and an emollient or lubricant. Frequently employed structuring agents include long chain alcohols, such as stearyl alcohol, and glyceryl ethers or esters and oligo(ethylene oxide) ethers or esters thereof. Thickeners and gelling agents include, for example, polymers of acrylic or methacrylic acid and esters thereof, polyacrylamides, and naturally occurring thickeners such as agar, carrageenan, gelatin, and guar gum. Examples of emollients include triglyceride esters, fatty acid esters and amides, waxes such as beeswax, spermaceti, or carnauba wax, phospholipids such as lecithin, and sterols and fatty acid esters thereof. The topical formulations may further include other components, e.g., astringents, fragrances, pigments, skin penetration enhancing agents, sunscreens (e.g., sunblocking agents), etc..

For an oral pharmaceutical formulation, suitable excipients include pharmaceutical grades of carriers such as mannitol, lactose, glucose, sucrose, starch, cellulose, gelatin, magnesium stearate, sodium saccharine, and/or magnesium carbonate. For use in oral liquid formulations, the composition may be prepared as a solution, suspension, emulsion, or syrup, being supplied either in solid or liquid form suitable for hydration in an aqueous carrier, such as, for example, aqueous saline, aqueous dextrose, glycerol, or ethanol, preferably water or normal saline. If desired, the composition may also contain minor amounts of non-toxic auxiliary substances such as wetting agents, emulsifying agents, or buffers.

By way of illustration, the fenfluramine composition can be admixed with conventional pharmaceutically acceptable carriers and excipients (i.e., vehicles) and used in the form of aqueous solutions, tablets, capsules, elixirs, suspensions, syrups, wafers, and the like. Such pharmaceutical compositions contain, in certain embodiments, from about <NUM>% to about <NUM>% by weight of the active compound, and more generally from about <NUM>% to about <NUM>% by weight of the active compound. The pharmaceutical compositions may contain common carriers and excipients, such as corn starch or gelatin, lactose, dextrose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride, and alginic acid. Disintegrators commonly used in the formulations of this invention include croscarmellose, microcrystalline cellulose, corn starch, sodium starch glycolate and alginic acid.

Particular formulations of the present disclosure are in a liquid form. The liquid may be a solution or suspension and may be an oral solution or syrup which is included in a bottle with a pipette which is graduated in terms of milligram amounts which will be obtained in a given volume of solution. The liquid solution makes it possible to adjust the solution for small children which can be administered anywhere from <NUM> to <NUM> and any amount between in half milligram increments and thus administered in <NUM>, <NUM>, <NUM>, <NUM>, etc..

A liquid composition will generally consist of a suspension or solution of the compound or pharmaceutically acceptable salt in a suitable liquid carrier(s), for example, ethanol, glycerine, sorbitol, non-aqueous solvent such as polyethylene glycol, oils or water, with a suspending agent, preservative, surfactant, wetting agent, flavoring or coloring agent. Alternatively, a liquid formulation can be prepared from a reconstitutable powder.

The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how to make and use the present invention, and are not intended to limit the scope of what the inventors regard as their invention nor are they intended to represent that the experiments below are all or the only experiments performed. Efforts have been made to ensure accuracy with respect to numbers used (e.g. amounts, temperature, etc.) but some experimental errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, molecular weight is weight average molecular weight, temperature is in degrees Centigrade, and pressure is at or near atmospheric. By "average" is meant the arithmetic mean. Standard abbreviations may be used, e.g., s or sec, second(s); min, minute(s); h or hr, hour(s); aa, amino acid(s); i. , intramuscular(ly); i. , intraperitoneal(ly); s. , subcutaneous(ly); and the like.

Many general references providing commonly known chemical synthetic schemes and conditions useful for synthesizing the disclosed compounds are available (see, e.g.,<NPL>; or <NPL>).

Compounds as described herein can be purified by any purification protocol known in the art, including chromatography, such as HPLC, preparative thin layer chromatography, flash column chromatography and ion exchange chromatography. Any suitable stationary phase can be used, including normal and reversed phases as well as ionic resins. In certain embodiments, the disclosed compounds are purified via silica gel and/or alumina chromatography. See, e.g., <NPL>; and <NPL>.

During any of the processes for preparation of the subject compounds, it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups as described in standard works, such as<NPL>, in <NPL>, in "<NPL>, in "<NPL>, in <NPL>, and/or in <NPL>. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

The subject compounds can be synthesized via a variety of different synthetic routes using commercially available starting materials and/or starting materials prepared by conventional synthetic methods. A variety of examples of synthetic routes that can be used to synthesize the compounds disclosed herein are described in the schemes below.

Table <NUM> summarizes the chemical and physical properties of Fenfluramine HCl.

Scheme <NUM> shows a <NUM>-step route of synthesis used to manufacture initial clinical supplies of Fenfluramine HCl from ketone (<NUM>). The batch size is <NUM> performed in laboratory glassware (kilo lab). No chromatography is required and the process steps are amenable to scale-up. In process <NUM> there is one isolated intermediate Fenfluramine Free Base (<NUM>) starting from commercially supplied <NUM>-(<NUM>-(trifluoromethyl)phenyl) acetone (Ketone <NUM>). All steps are conducted under cGMPs starting from Ketone (<NUM>).

Scheme <NUM> shows a <NUM>-step route of synthesis to Fenfluramine HCl that can be used for commercial supply. Route <NUM> utilizes the same <NUM>-step process used by Route <NUM> to convert Ketone (<NUM>) to Fenfluramine HCl with the exception that Ketone (<NUM>) is synthesized under cGMP conditions starting from <NUM>-(Trifluoromethyl)-phenyl acetic acid (Acid <NUM>). Bisulfate Complex (<NUM>) is an isolatable solid and can be purified before decomplexation to Ketone (<NUM>). In-situ intermediates which are oils are shown in brackets. Batch sizes of <NUM> are performed. Commercial batch sizes of <NUM> are performed in fixed pilot plant equipment. Steps <NUM>-<NUM> of Scheme <NUM> to manufacture Ketone (<NUM>) have been demonstrated on a <NUM> scale to provide high purity ketone (<NUM>) of ><NUM>% (GC & HPLC). Conversion of Ketone (<NUM>) to Fenfluramine using either Route <NUM> or <NUM> has provided similar purity profiles. <CHM>
Starting materials are designated by enclosed boxes. Bracketed and non bracketed compounds respectively indicate proposed in-situ and isolated intermediates. NMI = N-Methyl Imidazole.

A solution of ethylamine, water, methanol, and <NUM>-(<NUM>-(trifluoromethyl)phenyl) acetone (Ketone <NUM>) was treated with sodium triacetoxyborohydride and stirred for <NUM> at <NUM> at which time HPLC analysis (IPC-<NUM>; In Process Control No. <NUM>) showed the reaction to be complete and sodium hydroxide solution was added until pH ><NUM>. Toluene was added and the phases separated, and the aqueous phase (IPC-<NUM>) and organic phase (IPC-<NUM>) are checked for remaining Fenfluramine and Fenfluramine alcohol and the organic phase was reduced. Purified water was added and the pH adjusted to < <NUM> using conc. HCl and the phases were separated. The aqueous phase was washed with toluene and the toluene phase (IPC-<NUM>) and the aqueous phase (IPC-<NUM>) was checked for Fenfluramine and Fenfluramine alcohol content. The aqueous phase containing product is pH adjusted to ><NUM> using sodium hydroxide solution. The basic aqueous phase was extracted with MTBE until removal of Fenfluramine from the aqueous phase was observed by HPLC (<<NUM>/ml) (IPC-<NUM>). The organic phase was dried over sodium sulfate and filtered. The filtrate was concentrated in vacuo to give the intermediate product Fenfluramine Free Base <NUM> as a pale yellow oil tested per specifications described herein which showed by NMR the material to contain <NUM> % toluene giving an active yield of <NUM>% with a purity of <NUM> % by HPLC (<NUM> % Fenfluramine alcohol).

To a flask was charged ethanol and acetyl chloride. The solution was stirred slowly overnight before ethyl acetate was added. The HCl in ethyl acetate solution formed was polish filtered into a clean carboy and retained for later use. To a vessel was added Fenfluramine free base <NUM> and MTBE. The Fenfluramine solution in MTBE was collected in two carboys before the vessel was cleaned and checked for particulate residue. The Fenfluramine solution was polish filtered into a vessel and cooled and HCl in ethyl acetate solution was added giving a final pH of <NUM>-<NUM>. The batch was stirred for <NUM> and filtered. The product was dried under vacuum at <NUM>. The product (<NUM>% yield) was tested per IPC-<NUM> had a purity of <NUM>% by HPLC and GC headspace analysis showed MTBE (<NUM> ppm) and EtOAc (<NUM> ppm) to be present. The product was then tested per specifications shown herein.

Procedure: Charge acetic anhydride, (<NUM>. 8vol, <NUM>. 0wt, <NUM>. ) to a vessel and commence stirring. Cool the solution to -<NUM> to <NUM>, targeting -<NUM>. Charge <NUM>-methylimidazole, (<NUM>. 2vol, <NUM>. 21wt, <NUM>. ) to the mixture at -<NUM> to <NUM>. Caution: very exothermic. If necessary, adjust the temperature to <NUM> to <NUM>. Charge ZX008 acid, (<NUM>. 00wt, <NUM>. ) to the mixture at <NUM> to <NUM>. Caution: exothermic. Stir the mixture at <NUM> to <NUM> until ≤<NUM>% area ZX008 acid by HPLC analysis, typically <NUM> to <NUM> hours. Charge <NUM>% w/w sodium chloride solution (<NUM>. 0vol) to the mixture at <NUM> to <NUM>, <NUM> to <NUM> minutes. Caution: very exothermic which will be slightly delayed. Warm the mixture to <NUM> to <NUM> over <NUM> to <NUM> minutes and continue stirring for a further <NUM> to <NUM> minutes at <NUM> to <NUM>. Charge TBME, (<NUM>. 0vol, <NUM>. 7wt) to the mixture and stir for <NUM> to <NUM> minutes at <NUM> to <NUM>. Separate the aqueous layer and retain the organic layer. Back-extract the aqueous layer with TBME, (2x3.0vol, 2x2.2wt) at <NUM> to <NUM> retaining each organic layer. Adjust the pH of the combined organic layer to pH <NUM> to <NUM>, targeting <NUM> by charging <NUM>%w/w sodium hydroxide solution (<NUM> to <NUM>. 3vol) at <NUM> to <NUM>. Caution: exothermic. Separate the aqueous layer and retain the organic layer. Wash the organic layer with <NUM>%w/w sodium hydrogen carbonate solution (2x3.0vol) at <NUM> to <NUM>. Determine the residual ZX008 acid content in the organic layer by HPLC analysis, pass criterion ≤<NUM> % area ZX008 acid. Wash the organic layer with purified water, (2x3.0vol) at <NUM> to <NUM>. Concentrate the organic layer under reduced pressure to ca. 2vol at <NUM> to <NUM>, targeting <NUM>.

Determine the w/w assay of ZX008 ketone (WIP) in the mixture by <NUM>-NMR analysis for information only and calculate the contained yield of ZX008 ketone (WIP) in the mixture. Note: This step can be removed from the process since the process is robust and consistently delivers <NUM> to <NUM>%th yield. The achieved yield was factored into the charges of the subsequent steps.

Charge n-heptane, (<NUM>. 0vol, <NUM>. 7wt) to the mixture at <NUM> to <NUM>, targeting <NUM>. Concentrate the mixture to ca. 2vol at <NUM> to <NUM>, targeting <NUM>. Determine the TBME content in the mixture by <NUM>-NMR analysis, (pass criterion ≤ <NUM>%w/w TBME vs. ZX008 ketone). Charge n-heptane, (<NUM>. 4vol, <NUM>. 6wt) at <NUM> to <NUM>, targeting <NUM>, vessel A. To vessel B, charge sodium metabisulfite, (<NUM>. 82wt, <NUM>. ) at <NUM> to <NUM>. To vessel B, charge a solution of sodium hydrogen carbonate, (<NUM>. 16wt, <NUM>. ) in purified water, code RM0120 (<NUM>. 0vol) at <NUM> to <NUM> followed by a line rinse with purified water, code RM0120 (<NUM>. 4vol) at <NUM> to <NUM>. Caution: gas evolution. Heat the contents of vessel B to <NUM> to <NUM>, targeting <NUM>. Charge the contents from vessel A to vessel B followed by a line rinse with n-heptane, (<NUM>. 8vol, <NUM>. 5wt) at <NUM> to <NUM>, targeting <NUM>. Stir the mixture for <NUM> to <NUM> hours at <NUM> to <NUM>, targeting <NUM>. Charge n-heptane, code RM0174 (<NUM>. 2vol, <NUM>. 2wt) to the mixture with the temperature being allowed to cool to <NUM> to <NUM> at the end of the addition. Cool the mixture to <NUM> to <NUM> at approximately constant rate over <NUM> to <NUM> minutes. Stir the mixture at <NUM> to <NUM> for <NUM> to <NUM> hours.

Sample the mixture to determine the residual ZX008 ketone content by <NUM>-NMR analysis, (pass criterion ≤<NUM>%mol, target <NUM>%mol ZX008 ketone vs. ZX008 ketone bisulfite adduct). Filter the mixture and slurry wash the filter-cake with n-heptane, (2x2.0vol, 2x1.4wt) at <NUM> to <NUM>. Dry the solid at up to <NUM> until the water content is ≤<NUM>%w/w water by KF analysis according to AKX reagent. At least <NUM> hours. Determine the w/w assay of the isolated ZX008 ketone bisulfite adduct by <NUM>-NMR analysis and calculate the contained yield of ZX008 ketone bisulfite adduct.

Yields and Profiles: The yield for the stage <NUM> Demonstration batch is summarized Table below. Input: <NUM> uncorr. , acid, <NUM>% area (QC, HPLC), <NUM>-isomer not detected, <NUM>-isomer <NUM>% area, RRT1. <NUM> (previously not observed) <NUM>% area as per the preparative method. The analytical data is summarized in Table 1A below.

Procedure: Charge toluene, (<NUM>. 0vol, <NUM>. 3wt), and purified water, (<NUM>. 0vol) to the vessel and commence stirring. If necessary, adjust the temperature to <NUM> to <NUM> and charge ZX008 ketone bisulfite adduct, (<NUM>. 00wt corrected for %w/w assay ) to the mixture at <NUM> to <NUM>. Charge <NUM>%w/w sodium hydroxide solution to the mixture at <NUM> to <NUM> adjusting the pH of the mixture to pH <NUM> to <NUM>, targeting <NUM> (<NUM> to <NUM>.

Separate the lower aqueous layer and retain the top organic layer. Wash the organic layer with purified water, (<NUM>. 0vol) at <NUM> to <NUM>. Concentrate the organic layer under reduced pressure to ca. 2vol at <NUM> to <NUM>, targeting <NUM>. Charge methanol, (<NUM>. 0vol, <NUM>. 0wt) to the mixture at <NUM> to <NUM>, targeting <NUM>. Re-concentrate the mixture under reduced pressure to ca. 2vol at <NUM> to <NUM>, targeting <NUM>. Repeat steps <NUM> and <NUM> once before continuing with step <NUM>. Cool the mixture to <NUM> to <NUM>. Clarify the mixture into a tared, suitably-sized drum followed by a methanol (<NUM>. 0vol, <NUM>. 8wt) line rinse at <NUM> to <NUM>. Determine the w/w assay of ZX008 ketone (WIP) in the mixture by <NUM>-NMR analysis and calculate the contained yield of ZX008 ketone (WIP) in the mixture. Determine the toluene content in the mixture by <NUM>-NMR analysis.

Yields and Profiles: The yield for the step <NUM> Demonstration batch is summarized in Table 1B below. Input: <NUM> corr. Ketone bisulfite adduct, <NUM>%w/w assay (NMR, using DMB as internal standard in d<NUM>-DMSO), (<NUM>. 00eq, <NUM>. for w/w assay) for input calculation.

Procedure: Charge the ZX008 ketone (corr. for assay, <NUM>. 00wt, <NUM>. isolated as solution in MeOH in stage <NUM>) to a vessel. Charge methanol, code RM0036 (<NUM>. 0vol, <NUM>. 0wt) to the mixture at <NUM> to <NUM>. Cool the solution to <NUM> to <NUM>. Charge 70wt% aqueous ethylamine solution (<NUM>. 3vol, <NUM>. 6wt, <NUM>. 0eq) to the mixture at <NUM> to <NUM>, over <NUM> to <NUM> minutes, followed by a line rinse with methanol (<NUM>. 0vol, <NUM>. Warm the mixture to <NUM> to <NUM> and stir the mixture for a further <NUM> to <NUM> minutes at <NUM> to <NUM>. Adjust the mixture to <NUM> to <NUM> if required, targeting <NUM>. Charge sodium triacetoxyborohydride (<NUM>. 4wt, <NUM>. ) to the mixture in approximately <NUM> portions, keeping the mixture at <NUM> to <NUM>, targeting <NUM>. Addition time <NUM> to <NUM> hours. Caution: Exothermic. Stir the mixture at <NUM> to <NUM> until complete by HPLC analysis, pass criterion ≤<NUM>%area ZX008 ketone, typically <NUM> to <NUM> hours. Adjust the pH of the mixture to pH><NUM> by charging <NUM>%w/w aqueous sodium hydroxide solution (<NUM> to <NUM>. 0vol) to the mixture at <NUM> to <NUM>. Addition time <NUM> to <NUM> minutes. Caution: Exothermic. If necessary, adjust the temperature to <NUM> to <NUM>. Extract the mixture with toluene (3x3.0vol, 3x2.6wt) at <NUM> to <NUM>, retaining and combining the top organic layer after each extraction. Wash the combined organic layer with purified water, (<NUM>. 0vol) at <NUM> to <NUM>. Heat the mixture to <NUM> to <NUM>, targeting <NUM>. Concentrate the mixture under reduced pressure at constant volume maintaining ca. 5vol by charging the organic layer at approximately the same rate as the distillation rate at <NUM> to <NUM>, targeting <NUM>. Cool the mixture to <NUM> to <NUM>. Charge purified water (<NUM>. 0vol) to the mixture at <NUM> to <NUM>. Adjust the pH of the mixture to <NUM>≤pH≤<NUM> at <NUM> to <NUM> by charging concentrated hydrochloric acid, <NUM>. Do not delay from this step until neutralization.

Separate the layers at <NUM> to <NUM> retaining the bottom aqueous layer. Wash the aqueous layer with toluene, (<NUM>. 0vol, <NUM>. 6wt) at <NUM> to <NUM> retaining the aqueous layer. Adjust the pH of the aqueous layer to pH><NUM> by charging <NUM>%w/w sodium hydroxide solution at <NUM> to <NUM>. <NUM> to <NUM>. Caution: Exothermic. Charge TBME, code RM0002 (<NUM>. 0vol, <NUM>. 5wt) to the basic aqueous layer. Separate the layers at <NUM> to <NUM> retaining the top organic layer. Back-extract the aqueous layer with TBME (2x2.0vol, 2x1.5wt) at <NUM> to <NUM> retaining the organic layers. Wash the combined organic layer with purified water, (2x1.0vol) at <NUM> to <NUM>. Concentrate the combined organic layers under reduced pressure at <NUM> to <NUM>, targeting <NUM> to ca. Determine the residual toluene content of the mixture by <NUM>-NMR analysis. Sample for determination of residual water content by KF analysis, AKX reagent. Charge TBME (<NUM>. 7vol, <NUM>. 4wt) to the mixture at <NUM> to <NUM>. Cool the solution to <NUM> to <NUM>, targeting <NUM>. Charge concentrated hydrochloric acid (<NUM>. 54vol, <NUM>. 46wt) maintaining the temperature <<NUM>. Caution: Exothermic. Line rinse with TBME (<NUM>. 0vol, <NUM>. If necessary, adjust the temperature to <NUM> to <NUM> and stir the mixture at <NUM> to <NUM> for a further <NUM> to <NUM> hours. Filter the mixture and wash the filter-cake with TBME (2x4.4vol, 2x3.3wt) at <NUM> to <NUM>. Dry the solid at up to <NUM> until the TBME content is ≤<NUM>%w/w TBME by <NUM>-NMR analysis. <NUM> to <NUM> hours.

Yields and Profiles: The yield for the step <NUM> Demonstration batch is summarized in Table 1C below. Input: <NUM> corr. Ketone, <NUM>% w/w assay (NMR, using TCNB as internal standard in CDCl<NUM>), (<NUM>. 00eq, <NUM>. for w/w assay) for input calculation. <FIG> and Table 1D shows an exemplary HPLC chromatogram of a crude preparation of fenfluramine hydrochloride (<NUM> UV absorbance).

Procedure: Charge Fenfluramine. HCl (crude) (<NUM>. 00wt, <NUM>. ) and TBME (<NUM>. 0vol, <NUM>. 4wt) to the vessel and commence stirring. Heat the suspension to reflux (<NUM> to <NUM>). Charge ethanol (<NUM>. 0vol, <NUM>. 9wt) maintaining the temperature at <NUM> to <NUM>. Addition time <NUM> minutes. Stir at <NUM> to <NUM> for <NUM> to <NUM> minutes and check for dissolution. Stir the solution at <NUM> to <NUM> for <NUM> to <NUM> minutes, targeting <NUM> to <NUM>. Clarify the reaction mixture through a <NUM> in-line filter at <NUM> to <NUM>, followed by a line rinse with TBME (1vol, <NUM>. Cool the solution to <NUM> to <NUM>. Charge Fenfluramine. HCl, code FP0188 (<NUM>. Check for crystallization. Allow the suspension to cool to <NUM> to <NUM>, target <NUM> over <NUM> to <NUM> hours at an approximately constant rate. Stir the mixture at <NUM> to <NUM>, target <NUM> for <NUM> to <NUM> hours. Filter the mixture and wash the filter-cake with clarified TBME (2x3.0vol, 2x2.2wt) at <NUM> to <NUM>. Dry the solid at up to <NUM> until the TBME content is ≤<NUM>%w/w TBME and the ethanol content is <<NUM>%w/w EtOH by <NUM>-NMR analysis. <NUM> to <NUM> hours. Determine the w/w assay of the isolated Fenfluramine. HCl by <NUM>-NMR analysis.

Yields and Profiles: The yield for the stage <NUM> Demonstration batch is summarized in Table 1E below. Input: <NUM> uncorr. Fenfluramine. HCl crude (<NUM>. 00eq, <NUM> wt uncorr. ) for input calculation. <FIG> shows an exemplary HPLC chromatogram of a crystallized fenfluramine hydrochloride sample (<NUM> UV absorbance).

Table <NUM> summarizes the in-process controls (IPCs) by IPC number as cited in the narrative procedures above used for Process <NUM>.

This section provides information and specification controls for the starting materials used to produce clinical supplies of fenfluramine per the routes shown herein.

Table <NUM> provides a list of the intermediates for the Route <NUM> synthesis. Both routes share the same intermediate Fenfluramine Free Base (<NUM>). Fenfluramine Free Base (<NUM>) was treated as an isolated intermediate in the Route <NUM> process however the Route <NUM> process uses fixed equipment where both Ketone (<NUM>) and Fenfluramine Free Base <NUM>, both non-isolatable oils, are telescoped as a solution and controlled as in-situ intermediates. The Bisulfate Complex (<NUM>) is isolated as a solid thus is amenable to treatment as an isolated intermediate and released as such. Crude Fenfluramine HCl can be isolated as an intermediate before recrystallization.

A Specification and Testing Strategy for Intermediates is used. Additional tests and acceptance criteria are be added based upon review of data from the primary stability batches and process validation critical parameter studies. Analytical reference standards are used in full characterization of each intermediate. HPLC methods to determine assay and impurities are the same as the drug substance release method and are validated for Accuracy, Precision: Repeatability, Intermediate Precision, Selectivity / Specificity, Detection limit, Quantitation limit, Linearity, Range, and Robustness.

Fenfluramine HCl is developed as a single polymorph Form <NUM>. A polymorphism and pre-formulation study has been conducted. Under a wide range of solvents and conditions crystalline material is produced of the same polymorph Form <NUM> based on a well-defined XRPD pattern and a consistent reproducible endotherm by DSC analysis. A summary of the chemophysical properties of Fenfluramine HCl from this study is provided below. Tabulated data includes example diffractograms, DSCs, and micrographs.

The input Fenfluramine HCl (from precipitative isolation) was characterized to provide reference data and also to determine if the salt was of the same form as that identified from previous salt formations. The XRPD pattern of the salt reveals a crystalline solid that visually matches the reflection patterns obtained from formal crystallization of Fenfluramine HCl and has been arbitrarily termed Form <NUM>. Comparison of the µATR-FTIR data for the salt from various batches gave profiles that had a <NUM>% match.

Thermal data analysis matched previous data obtained with only one major endotherm on the DSC thermograph peaking at <NUM> that matches the beginning of potential decomposition shown in a TGA thermograph. This also matches the reported melting point quoted for the reference standard.

Isolation of the amorphous form has been shown to be difficult, with attempts using three common methods (rapid solvent evaporation, anti-solvent precipitation and lyophilization) all yielding highly crystalline solids that very closely share the same XRPD pattern of the input Form <NUM>.

Stability analysis of the salt after one week at <NUM>/<NUM>% RH, three weeks at <NUM>/<NUM>% RH, and under photostability conditions revealed that the input Form <NUM> has been maintained with no new impurities observed at <NUM>% threshold.

Results from DSC heat cycling analysis of Fenfluramine HCl are comparable to results generated when the material was held at <NUM>. No crystallization event was noted and the amorphous was not generated but rather Form <NUM> was returned.

Holding Fenfluramine HCl at approximately <NUM> for several hours causes a melt and evaporation event to take place with recombination and cooling to provide a white solid. Analysis of the white solid by XRPD, DSC and <NUM>H NMR indicates no change in chemical or physical form, purity, or dissociation.

Forced degradation studies carried out have proven Fenfluramine HCl to be stable under a range of conditions. Thermal modulation of Fenfluramine HCl repeatedly yielded the input Form <NUM>.

Impurities in a drug substance can be organic impurities (process impurities or drug substance-related degradants), inorganic impurities (salt residues or metals) and residual solvents; some of these impurities must be evaluated as to whether or not they are genotoxic agents. These impurities are taken into consideration and controlled in Fenfluramine HCl preparation by using either compendia or validated analytical methods per the specifications or by separate "for information only" testing. The following sections address the actual and potential impurities in Fenfluramine HCl.

No impurities reported in cGMP drug substance batches intended for use in humans have exceeded the ICHQ3A qualification thresholds of <NUM>% (Table <NUM>) All impurities > <NUM>% are identified and handled as described in ICH Q3A unless they are genotoxic impurities.

Table <NUM> lists the known potential impurities arising from the route of synthesis. All of these impurities are controlled to below ICHQ3A qualification threshold of <NUM>% by either process changes and/or control of starting material input purities.

No change in impurity profile is observed upon long-term storage based on forced degradation studies under the ICH Q1A(R2) conditions of heat (solid, solution), acid, base, oxidizing, and ICH Q1B photostability conditions (solid, solution). Fenfluramine HCL is stable for <NUM> days as a solid at <NUM> (<NUM> parent area %), as a solution in water-acetonitrile at <NUM> (<NUM> parent area %), as a solution in acid, base, or photosensitizing conditions at ambient. Only oxidizing conditions (peroxide conditions) produced degradation of Fenfluramine HCl to <NUM>% after <NUM> day producing several new related substances at ~<NUM>% each consistent by LC-MS with +<NUM> oxidation by-products.

Table <NUM> in the Batch Analysis section summarizes the solvents used in the process and the resulting amounts found in drug substance. All solvents used in the GMP steps are controlled at ICH Q3A limits using a suitably qualified Head-Space (HS) GC method.

Heavy Metals conform to either USP <<NUM>> or ICP method USP <<NUM>> as well as ICH Q3D.

The ICH guidelines Q3A and Q3B are not sufficient to provide guidance on impurities that are DNA-reactive. The <NPL>) and the <NPL>" (ICH Guideline M7) are taken into consideration in controlling for potential genotoxic impurities. The diazonium route to prepare ketone (<NUM>) described in <FIG> has a disadvantage due to the potential formation of genotoxic intermediates shown as boxed compounds (e.g., N-hydroxyaryl, N-nitrosamine and Nitro compound). Muller et al. (Regulatory Toxicology and Pharmacology <NUM> (<NUM>) <NUM>-<NUM>) list potential functional alert groups that can be genotoxic. Safety guidances and regulations indicate that analysis of a process and identification of potential genotoxic agents, and control of such impurities at sub <NUM> parts per million levels is critical for safety. Often removal of such impurities and/or demonstrating their absence is costly and time consuming and sometimes difficult to achieve technically. For these reasons, selecting synthetic routes that circumvent the potential for such toxic intermediates is important. Because of the potential problems with the diazo route discussed above, as well as potential safety issues using diazo (shock-sensitive) intermediates, as well as the lower purity profiles with this route, this route is less preferred than the preferred route to ketone (<NUM>) starting from Nitrile (<NUM>). This route produces no potential genotoxic agents and leads to high purity Ketone (<NUM>) after isolation by distillation or via the bisulfite salt adduct - hydrolysis sequence.

Additionally, attempts to remove isomer by-products present in commercial supplies of Aniline were unsuccessful whereas crystallization the Acid (<NUM>) resulting from hydrolysis of the nitrile (<NUM>) provides crystalline Acid (<NUM>) which can be purified to remove isomers early in synthesis. Removing impurities and/or isomers early in a synthesis is preferred if it is known such impurities track to final product, as the need to crystalize a final product at the end of a synthesis is more costly in losses and impacts cost of goods more greatly than removing early in synthesis before raw materials are invested along the process.

The impurities formed during the Dakin-West chemistry and their subsequent removal using the distillation or via isolation of the product ketone as the bisulfite salt are described. The two major impurities found are shown below.

Table <NUM> shows a table of analytical data for crude Ketone (<NUM>) isolated from Dakin-West reaction before and after bisulfite purification. In entry <NUM> crude Ketone isolated directly from the Dakin-West step (pre-bisulfite treatment) is <NUM>% purity (e.g. about <NUM>%) and contains <NUM>% (e.g., about <NUM>%) and <NUM>% (e.g., about <NUM>%) respectively of impurities having RRTs <NUM> and <NUM>, which are proposed to be the acetate and dimer impurities (e.g., depicted above), respectively. In entry <NUM> which is post bisulfite treatment these are other impurities are removed leading to an overall purity of <NUM>% (e.g., about <NUM>%). Other entries shown in Table <NUM> provide other examples of this impurity enhancement by bisulfite treatment of crude Dakin-West ketone. The last two entries use pure Fluorchem ketone as input to the salt formation step and re-isolation of ketone thus illustrating that the salt formation and re-isolation does not produce any impurities itself. Additionally use of bicarbonate extraction procedure during reaction workup provides an improvement in purity of the resulting composition as it serves to remove any unreacted acid. Crude Ketone (<NUM>) made by the Diazo route showed similar improvements in purity when treated with bisulfite and isolated.

<NUM> of water and <NUM> of <NUM>% (w/w) aqueous hydrochloric acid are put in a flask equipped with stirrer and dropping funnel. <NUM> Grams (<NUM> moles) of m-trifluoromethylaniline are added after having cooled to <NUM> degree C with an ice bath and then, at <NUM> degree C, an aqueous solution containing <NUM> (<NUM> moles) of sodium nitrite in <NUM> of water is slowly added. The reaction mixture is stirred for <NUM> minutes and then is poured during <NUM> minutes into a mixture made by <NUM> of water, <NUM> (<NUM> moles) of cuprous chloride, <NUM> (<NUM> moles) of cupric chloride dihydrate, <NUM> of acetone, <NUM> (<NUM> moles) of sodium acetate trihydrate and <NUM> (<NUM> moles) of isopropenyl acetate while keeping the reaction temperature at <NUM> degree C. After further <NUM> minutes of stirring, the reaction mixture is brought to <NUM> degree C. , <NUM> of methylene chloride are added and the two layers are separated.

The aqueous layer is discarded while the organic layer is concentrated under vacuum until an oil is obtained which is treated with <NUM> of sodium metabisulfite, <NUM> of water and <NUM> of heptane under stirring at room temperature for <NUM> hours. The suspension is filtered, the bisulfite complex is washed on the filter with <NUM> of heptane and then suspended in a two-phase mixture made by <NUM> of methylene chloride and <NUM> of a <NUM>% (w/v) aqueous solution of sodium hydroxide. The layers are separated after one hour of stirring at room temperature, the aqueous phase is discarded while the organic layer is washed with water and evaporated under vacuum to give pure ketone.

Claim 1:
A method of preparing a fenfluramine active pharmaceutical ingredient, the method comprising:
reacting a <NUM>-(<NUM>-(trifluoromethyl)phenyl)acetic acid composition with acetic anhydride and a catalyst to produce a <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition; and
reductively aminating the <NUM>-(<NUM>-(trifluoromethyl)phenyl)propan-<NUM>-one composition with ethylamine using a borohydride reducing agent to produce a fenfluramine composition.