Patent Description:
For anti-falling glass slides available in the market, including positively charged anti-falling glass slides and lysine-treated anti-falling glass slides, and so on, anti-falling treatment is performed on the surface of the glass slides to improve the anti-falling performance of the slides. Those anti-falling glass slides have good anti-falling effects for paraffin sections and frozen sections. However, even the very expensive anti-falling glass slides are not able to resolve the problem of falling of the hard tissue slice. Such anti-falling glass slides are quite expensive, but anti-falling effect of the hard tissue slice is not obvious, and falling rate is still as high as <NUM>% to <NUM>%.

Since the hard tissue slice has a high dyeing falling rate, the dyeing success rate of the hard tissue slice is greatly reduced, so that the development of dyeing of the hard tissue slice is hindered. Thus, high dyeing falling rate of the hard tissue slice has always been a major obstacle to the development and progress of the hard tissue slice.

Therefore, one of the important areas of research is to provide a technical means through which dyeing falling rate of the hard tissue slice is reduced, and the dyeing success rate of the hard tissue slice is improved.

<CIT>, <CIT>, <CIT>, or <CIT> disclose system for tissue treatment involving liquid.

Given that the foregoing problems exist in the prior art, the present invention provides a hard tissue slice anti-falling pretreatment device and an anti-falling pretreatment method.

According to the first aspect of the invention, the invention provides, according to claim <NUM>, a hard tissue slice anti-falling pretreatment device, comprising a box and a slice frame, a buffer plate is arranged in the box, and a plurality of small holes are evenly formed in the buffer plate; a box cover is arranged above the box, a bottom of the box is communicated with a liquid discharge pipe through a liquid outlet, and the liquid discharge pipe is provided with a valve;.

Preferably, the number of the layers of transverse support frames is in a range from <NUM> to <NUM>.

Preferably, the bottom of the box extends downwards from the liquid outlet to form a liquid discharge passage, a lower end of the liquid discharge passage is communicated with the liquid discharge pipe.

Preferably, a diameter of a lower end of the liquid discharge passage is gradually reduced , so that it equals to a diameter of the liquid discharge pipe.

Preferably, the box and the slice frame are made of glass, stainless steel or polytetrafluoroethylene.

Preferably, a valve seat is arranged on the liquid discharge pipe, the valve is sleeved on the valve seat, a through-hole is formed in the valve, when the valve is rotated inside the valve seat, the through-hole is communicated with or disconnected from the liquid discharge pipe.

Preferably, each of the plurality of small holes formed in the buffer plate has a diameter in a range from <NUM> to <NUM>.

According to the second aspect of the invention, the invention provides a method for anti-falling pre-treating hard tissue slice using the anti-falling pretreatment device described above, comprising the steps of:.

By adopting the above-mentioned technical solutions, compared to the prior art, the present invention has the following technical effects:.

List of reference numeral in the drawings:
<NUM> box, <NUM>-buffer plate, <NUM>- box cover, <NUM>-liquid discharge outlet, <NUM>-slice frame, <NUM>-longitudinal support frame, <NUM>-vertical support rod, <NUM>-second support rod, <NUM>-transverse support frame, <NUM>-first support rod, <NUM>-liquid discharge pipe, <NUM>-valve.

When making slices or ultrathin slices, it is difficult to make slices with uniform thickness due to the fact that tissue is soft or tissue is partially soft or hard. Therefore, it is necessary to allow the interior of the tissue to be soaked with a certain material, so that uniform hardening of the entire tissue can be achieved to facilitate cutting into slices. Of note, the material here is called an embedding agent. Generally, paraffin, collodion, charcoal wax, gelatin, for example, are adopted as the embedding agent for the slices observed by an optical microscope; while epoxy resin, polystyrene resin, isobutylene resin and water-soluble resin are adopted as the embedding agent for the slices observed by an electron microscope.

Slices for HE staining, special staining or immunohistochemical staining must be subjected to the plastic removal process in advance. The falling rate of the hard tissue slice during its dyeing process is as high as <NUM>% after it is subjected to plastic removal process by using the conventional method. Pretreatment process carried out before the dyeing process is improved by using a hard tissue slice anti-falling pretreatment device as shown in <FIG> for the purpose of achieve anti-falling of the hard tissue slice.

The invention is illustrated in full details with reference to particular embodiments, so as to better understand the present invention, however, the invention is not limited to the following embodiments.

As shown in <FIG>, this embodiment provides a hard tissue slice anti-falling pretreatment device, comprising a box <NUM> and a slice frame <NUM>.

As shown in <FIG>, a buffer plate <NUM> is arranged in the box <NUM> for carrying the slice frame <NUM>. In a particular embodiment, the buffer plate <NUM> is a plate-like structure in which a plurality of holes are formed, and it is horizontally fixed at a lower part of the box <NUM>, the buffer plate <NUM> divides the box <NUM> into an upper space and a lower space. Liquid flowing through the top of the box <NUM> flows from the upper space to the lower space through the holes in the buffer place <NUM>. Preferably, each of the plurality of small holes formed in the buffer plate <NUM> has a diameter in a range from <NUM> to <NUM>. In the present invention, the buffer plate <NUM> is arranged in the box <NUM>, and a plurality of holes are densely formed in the buffer plate, so that flow direction of plastic removal liquid, anhydrous ethanol-acetone mixture and anhydrous ethanol is changed when they are discharged; through the guidance of the small holes, liquid in the box <NUM> only forms small eddies with soft undulations when flowing downwards, and large fluid fluctuation will not be created inside the box, so that anti-falling of the hard tissue slice can be achieved. If the buffer plate <NUM> is not provided, liquid is discharged only though the liquid discharge outlet at the bottom of the box <NUM>. In this case, a larger fluid spiral will be generated, strong fluid fluctuation is created in the box, which results in the falling of the slice in subsequent operations.

In this embodiment, as shown in <FIG>, the buffer plate <NUM> is horizontally fixed at a distance of <NUM> from the bottom of the box. The buffer plate <NUM> has a thickness of <NUM>. The size is only a particular embodiment of the invention, and the invention is not limited to the above-mentioned size.

In a preferred embodiment, the box <NUM> is integrally formed with the buffer plate <NUM>.

In this embodiment, as shown in <FIG>, a box cover <NUM> is arranged above the box <NUM>, the bottom of the box <NUM> is communicated with a liquid discharge pipe <NUM> through a liquid outlet <NUM>, and the liquid discharge pipe <NUM> is provided with a valve <NUM>; as shown in <FIG>, a valve seat is arranged on the liquid discharge pipe <NUM>, the valve <NUM> is sleeved on the valve seat; as shown in <FIG>, a through-hole is formed in the valve <NUM>, a knob on the valve <NUM> is turned so that the valve <NUM> is rotated in the valve seat. If it is necessary to discharge the liquid from the box <NUM>, the valve <NUM> is rotated until the through-hole is communicated with the liquid discharge pipe. In addition, flow of the discharging liquid can be adjusted by turning the knob on the valve <NUM>, so as to further control the flow of fluid in the box <NUM>.

Specifically, as shown in <FIG>, the box has a length of <NUM>, a width of <NUM>, an internal width of <NUM>, a wall thickness of <NUM>, and a height of <NUM>; the above dimensions are only a specific embodiment of the present invention, and the present invention is not limited to the above-mentioned dimensions.

In this embodiment, as shown in <FIG>, the liquid discharge pipe <NUM> has a diameter of <NUM>, and the dimension is only a specific embodiment of the present invention, and the present invention is not limited to the above-mentioned dimension.

In this embodiment, as shown in <FIG>, the slice frame <NUM> comprises two longitudinal support frames <NUM> and a plurality of layers of transverse support frames <NUM> fixed between the two longitudinal support frames <NUM>. As shown in <FIG>, each layer of transverse support frame <NUM> comprises a plurality of parallel first support rods <NUM>; in particular, in <FIG>, each layer of transverse support frame <NUM> comprises four first support rods <NUM>, the distance between two adjacent first support rods <NUM> is <NUM>, the farthest distance between two first support rods <NUM> is <NUM>, and each of the transverse support frames has a length of <NUM>. As shown in <FIG>, the distance between two adjacent layers of transverse support frames <NUM> is <NUM>, each of the first support rods <NUM> has a height of <NUM>, a straight section of the first support rod <NUM> has a length of <NUM>. The above dimensions are only a specific embodiment of the present invention, and the present invention is not limited to the above-mentioned dimensions.

In this embodiment, as shown in <FIG>, two ends of each longitudinal support frame <NUM> are each provided with a vertical support rod <NUM>, a plurality of second support rods <NUM> are fixed in parallel between the two vertical support rods <NUM>, the same ends of the plurality of first supports rods <NUM> at the same layer are fixed on same second support rod <NUM>. As shown in <FIG>, each of the vertical support rods <NUM> has a height of <NUM>, a width of <NUM>, and the slice frame <NUM> has a total width of <NUM>. The above dimensions are only a specific embodiment of the present invention, and the present invention is not limited to the above-mentioned dimensions.

In this embodiment, as shown in <FIG>, one end of the first support rod <NUM> is fixed to a side, facing the first support rod <NUM>, of the second support rod <NUM>, and the other end of the first support rod <NUM> extends upwards to form a baffle and it is fixed to a lower surface of the second support rod <NUM>, the gradient of each first support rod <NUM> relative to the horizontal plane is <NUM>-<NUM> degrees, the end, to which the baffle is fixed, is lower than the other end, that is, the heights of the second support rod <NUM> at two ends of the same first support rod <NUM> can be the same or can be varied. In a preferred embodiment, the baffle at the lower side has a height of <NUM> to <NUM>, so as to ensure the slice will not easily slide out of a track. The baffle can be arc-shaped or can be perpendicular to the first support rods <NUM>.

In this embodiment, as shown in <FIG>, the number of the layers of transverse support frames <NUM> is in a range from <NUM> to <NUM>. Further preferably, the number is <NUM>, and the height between two layers is <NUM>. The height can be selected freely according to the number of the glass slides in different applications.

In this embodiment, as shown in <FIG>, in a preferred embodiment, the bottom of the box <NUM> extends downwards from the liquid discharge outlet <NUM> to form a liquid discharge passage, a diameter of a lower end of the liquid discharge passage is gradually reduced, so that it equals to a diameter of the liquid discharge pipe, the lower end of the liquid discharge passage is communicated with the liquid discharge pipe <NUM>.

In a particular embodiment, the box <NUM> and the slice frame <NUM> are made of glass, stainless steel or polytetrafluoroethylene. Furthermore, for the sake of costs, they are made of glass.

In a preferred embodiment, the length, width, and height of the box <NUM>, the length, width, and height of the slice frame <NUM>, and the number of layers can be selected according to the number of slices to be processed.

The invention provides a method for anti-falling pretreatment of hard tissue slice using the anti-falling pretreatment device provided in Example <NUM>, wherein, for example, the pretreatment is done in prior to the HE staining of the hard tissue slice, the method comprises the steps of:.

Claim 1:
A hard tissue slice anti-falling pretreatment device, comprising a box (<NUM>) and a slice frame (<NUM>), wherein the slice frame (<NUM>) is used for placing one or more slides on which the hard tissue slice is attached, a buffer plate (<NUM>) is arranged in the box (<NUM>), and a plurality of small holes are evenly formed in the buffer plate (<NUM>); a box cover (<NUM>) is arranged above the box (<NUM>), a bottom of the box (<NUM>) is communicated with a liquid discharge pipe (<NUM>) through a liquid outlet (<NUM>), and the liquid discharge pipe (<NUM>) is provided with a valve (<NUM>); the slice frame (<NUM>) is arranged on the buffer plate (<NUM>) and comprises two longitudinal support frames (<NUM>) and a plurality of layers of transverse support frames (<NUM>) fixed between the two longitudinal support frames (<NUM>); each layer of transverse support frame (<NUM>) comprises a plurality of first support rods (<NUM>), the gradient of each first support rod (<NUM>) relative to the horizontal plane is <NUM>-<NUM> degrees, the end, with the low horizontal height, of each first support rod (<NUM>) extends upwards to form a baffle, and the other end of each baffle is fixed to a second support rod (<NUM>);
two ends of each longitudinal support frame (<NUM>) are each provided with a vertical support rod (<NUM>), a plurality of second support rods (<NUM>) are fixed in parallel between the two vertical support rods (<NUM>), the same ends of the plurality of first supports rods (<NUM>) at the same layer are fixed on same second support rod (<NUM>).