Patent Description:
Deterioration in thinking, memory, behavior, and the ability to perform activities of daily living falls under the broader domain of dementia. Alzheimer's disease (AD) dementia is the most common form of dementia. Other major forms include Lewy body dementia (LBD), vascular dementia (VD), Parkinson's disease (PD) dementia, dementia associated with schizophrenia, and frontotemporal dementia (FTD). Although they are pathologically different from each other, they have symptoms that are common and mixed forms often co-exist. Behavioral and psychological symptoms are one of the common symptoms observed across the forms of dementia which represent a heterogeneous group of non-cognitive symptoms and behaviors. Behavioral and psychological symptoms of dementia (BPSD) are also known as neuropsychiatric symptoms. BPSD constitutes a major component of the dementia syndrome irrespective of its subtype. It is estimated that BPSD affects up to <NUM>% of all dementia subjects over the course of their illness, and is independently associated with poor outcomes, including distress among patients and caregivers, early and long-term institutionalization, misuse of medication, and increased health care costs. They are clinically as relevant as cognitive symptoms because they strongly correlate with the degree of functional and cognitive impairment. BPSD includes agitation/aggression, aberrant motor behavior, aberrant vocalizations, anxiety, elation, dysphoria, irritability, depression, apathy, disinhibition, delusions, hallucinations, and sleep or appetite changes. Clinical evidences suggest that there is a broad overlap between the behaviorally relevant circuits and networks associated with these symptoms. The Neuropsychiatric Inventory (NPI) scale is widely used to assess neuropsychological symptoms in patients with dementias.

Psychosis symptoms are common across dementia types with a prevalence of <NUM>% to <NUM>%. In addition to occurring in patients with AD, psychosis also occurs in patients with other dementias of a wide variety of etiologies (<NPL>). Among patients with AD psychosis, it is reported that an increased occurrence of severe psychosis is associated with an increased presence of delusions and hallucinations as well as symptoms of agitation/aggression. Currently, no pharmacological treatment is approved for patients with AD psychosis, particularly, patients experiencing severe psychotic symptoms.

Agitation/aggression is highly prevalent in patients with dementia which involves emotional distress for patients and caregivers, risk of institutionalization, and faster rate of disease state progression. Individual items of agitation/aggression, aberrant motor behavior, aberrant vocalizations, irritability/lability, anxiety, and disinhibition from the NPI may represent agitation/aggression. Currently, no pharmacological treatment is approved for the treatment of agitation/aggression in patients with dementia. Apathy is also commonly observed in dementia and is a primary cause of caregiver distress. Apathy is characterized by lack of motivation, decreased initiative, akinesia, and emotional indifference. Currently, no pharmacological treatment is approved for the treatment of apathy in patients with dementia. Depression is also common in the dementia population. Although antidepressants are used for the management, evidence for utility is mixed. A high prevalence of sleep disorders is also observed in demented patients.

Combination of non-pharmacological and careful use of pharmacological interventions is the recommended therapeutic strategy for managing BPSD. Published literature indicates that modest symptomatic benefit is associated with short-term treatment of patients with BPSD using atypical antipsychotic agents like risperidone, olanzapine, and aripiprazole. However, benefits for longer-term treatment are less clear (<NPL>). Moreover, the modest benefits must be balanced against significant safety concerns associated with these drugs, including risks of accelerated cognitive decline, stroke, and death, particularly with longer-term use. Atypical antipsychotics have been associated with statistically significant acceleration of cognitive deterioration in patients with AD. Pimavanserin, a <NUM>-HT2A receptor antagonist which was recently approved for PD psychosis had shown statistically significant effect on Neuropsychiatric Inventory Nursing Home Version (NPI-NH) psychosis scale at week <NUM>, however, no effect was for agitation in AD study (<NPL>; NCT02992132). Citalopram (<NUM> daily dose) for agitation in AD study showed a significant decrease in agitation in <NUM> patients with AD (<NPL>). Worsening of cognition and QT interval prolongation were seen in citalopram treatment group.

Given the modest efficacy of current therapies, there is an urgent need to identify novel pharmacological mechanisms through which one can address the current limitations like side effects, cognitive worsening while treating BPSD.

<CIT> discloses process for large scale production of <NUM>-[(<NUM>-bromophenyl)sulfonyl]-<NUM>-methoxy-<NUM>-[(<NUM>-methyl-<NUM>-piperazinyl)methyl]-<NUM>-indole dimesylate monohydrate.

<CIT> discloses a combination of pure <NUM>-HT<NUM> receptor (<NUM>-HT<NUM>R) antagonists and acetylcholinesterase inhibitors for the treatment of cognitive disorders.

<CIT> discloses a combination of pure <NUM>-HT<NUM>R antagonists and NMDA antagonist for the treatment of cognitive disorders.

<CIT> discloses a combination of pure <NUM>-HT<NUM>R antagonists, acetylcholinesterase inhibitors and NMDA antagonist for the treatment of cognitive disorders.

The above patent publications do not disclose Masupirdine, in combination with an acetylcholinesterase inhibitor or NMDA antagonist for use in a method of treating behavioral and psychological symptoms in a patient with dementia.

<CIT> discloses the uses of a pure <NUM>-HT<NUM>R antagonist, specifically <NUM>-[(<NUM>-Bromophenyl)sulfonyl]-<NUM>-methoxy-<NUM>-[(<NUM>-methyl-<NUM>-piperazinyl)methyl]-<NUM>-indole or a pharmaceutically acceptable salt thereof. However, this patent application did not disclose the combination of <NUM>-HT<NUM>R antagonists and acetylcholinesterase inhibitors for the treatment of behavioral and psychological symptoms in patients with dementia.

Co-treatment of 5HT<NUM>R antagonist, SUVN-<NUM>, with donepezil and memantine significantly potentiated the procognitive effects when compared with a combination of memantine and donepezil in object recognition task (<NPL>). Co-treatment of SUVN-<NUM> with donepezil and memantine significantly potentiated the procognitive effects as compared to memantine and donepezil treatment alone in the Morris water maze task (<NPL>). SUVN-<NUM> is in development as a novel approach in the symptomatic treatment of AD dementia as a triple therapy with SUVN-<NUM> added to background treatment with donepezil and memantine (<NPL>; and <NPL>). The above publications do not disclose Masupirdine in combination with donepezil or memantine for use in a method of treating behavioral and psychological symptoms in a patient with dementia.

The <NUM>-HT<NUM>R antagonists had shown the activity on one of the NPI domain that is apathy (<CIT>). There is no information on the potential utility on other important domains of NPI in this said <CIT>. The NPI domains though are clubbed under single scale but the causative biological reasons for each of these symptoms are different. A person skilled in the art would not expect that if a <NUM>-HT<NUM>R antagonist is active in one domain, that means it will show activity in other domains as well. The effect of donepezil is mainly observed in the NPI domain namely apathy (<NPL>). Further, donepezil was evaluated for the treatment of agitation/aggression in patients with Alzheimer's disease, however, donepezil failed to show significant effect compared to placebo (<NPL>).

The NMDA receptor antagonist, memantine is expected to work by blocking the glutamate in the brain (<NPL>) whereas the <NUM>-HT<NUM>R antagonists work by increasing the glutamate levels in the brain in addition to other mechanisms including increasing acetylcholine levels (<NPL>). Thus given these contrasting mechanisms it is not anticipated that the combination of a pure <NUM>-HT<NUM>R antagonist and NMDA receptor antagonist would show coherence or positive effects in their pharmacological activity in the domains of BPSD.

Masupirdine (<NUM>-[(<NUM>-bromophenyl)sulfonyl]-<NUM>-methoxy-<NUM>-[(<NUM>-methyl-<NUM>-piperazinyl)methyl]-<NUM>-indole) is a pure <NUM>-HT<NUM>R antagonist with high selectivity over closely related serotonin subtypes.

The instant invention provides masupirdine or the pharmaceutically acceptable salt(s) thereof, in combination with donepezil or memantine, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia. The present invention is based on the unexpected and surprising finding that the combination of pure <NUM>-HT<NUM>R antagonist, masupirdine, and the compounds which act as acetylcholinesterase inhibitor such as donepezil or NMDA receptor antagonist, memantine addresses in patients with dementia an array of behavioral and psychological symptoms like agitation/aggression, delusions, hallucinations, or delusions and hallucinations. Based on these results, one can infer that such combined administration and/or co-treatment of masupirdine with donepezil or memantine, results in improved therapeutic efficacy in humans. Further, the pure <NUM>-HT<NUM>R antagonist, masupirdine or the pharmaceutically acceptable salt(s) thereof of the instant invention is more effective in combination with donepezil or memantine in the treatment of agitation/aggression, delusions, hallucinations, or delusions and hallucinations.

Any subject-matter falling outside the scope of the claims is provided for information purposes only. Any references in the description to methods of treatment refer to the compounds, pharmaceutical compositions and medicaments of the present invention for use in a method for treatment of the human or animal body by therapy.

The present invention provides masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil or memantine, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia, comprising administering an effective dose of masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil or memantine, or a pharmaceutically acceptable salt thereof.

In an aspect, the present invention relates to masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil or memantine, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations in a patient with Alzheimer's disease dementia, dementia associated with schizophrenia, Parkinson's disease dementia, Lewy body dementia, vascular dementia, and frontotemporal dementia, comprising administering an effective dose of masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil or mementine, or a pharmaceutically acceptable salt thereof.

In an embodiment, the present invention relates to masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In an embodiment, the present invention relates to masupirdine, or a pharmaceutically acceptable salt thereof, in combination with memantine, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof, in combination with memantine, or a pharmaceutically acceptable salt thereof.

Unless otherwise stated, the following terms used in the specifications and claims have the meanings as given below:
The term, "<NUM>-HT<NUM> receptor antagonist" as used herein refers to a ligand or drug that shows affinity towards <NUM>-HT<NUM> receptor, blocks or inhibits the function/ binding of <NUM>-HT<NUM> receptor agonist.

The term, "pure <NUM>-HT<NUM> receptor antagonist" as used herein refers to a <NUM>-HT<NUM> receptor antagonist which has very high selectivity (><NUM> fold) over closely related serotonin subtypes like <NUM>-HT1A, <NUM>-HT1B, <NUM>-HT1D, <NUM>-HT2A, <NUM>-HT2C, <NUM>-HT<NUM>, <NUM>-HT5A, and <NUM>-HT<NUM>.

Example of pure <NUM>-HT<NUM> receptor antagonist is (<NUM>-[(<NUM>-Bromophenyl)sulfonyl]-<NUM>-methoxy-<NUM>-[(<NUM>-methyl-<NUM>-piperazinyl)methyl]-<NUM>-indole).

The international nonproprietary name (INN) for (<NUM>-[(<NUM>-Bromophenyl)sulfonyl]-<NUM>-methoxy-<NUM>-[(<NUM>-methyl-<NUM>-piperazinyl)methyl]-<NUM>-indole) is masupirdine.

Example of pharmaceutically acceptable salt of above pure <NUM>-HT<NUM> receptor antagonist, masupirdine is dimesylate monohydrate.

Masupirdine dimesylate monohydrate is also known as SUVN-<NUM> which has the chemical structure as shown below.

Masupirdine is commonly administered as masupirdine dimesylate monohydrate. The compound, masupirdine dimesylate monohydrate and its preparation were described in <CIT>, <CIT> and in article <NPL>.

The term, "active ingredient" or "active compound(s)" or "compound(s)" as used herein refers to a <NUM>-HT<NUM> receptor antagonist or an acetylcholinesterase inhibitor or NMDA receptor antagonist. Preferably the <NUM>-HT<NUM> receptor antagonist is (<NUM>-[(<NUM>-Bromophenyl)sulfonyl]-<NUM>-methoxy-<NUM>-[(<NUM>-methyl-<NUM>-piperazinyl)methyl]-<NUM>-indole) or a pharmaceutically acceptable salt, the acetylcholinesterase inhibitor is donepezil, or pharmaceutically acceptable salts thereof and NMDA receptor antagonist is memantine or pharmaceutically acceptable salts thereof.

The term, "acetylcholinesterase inhibitor" as used herein is a chemical or drug that inhibits acetylcholinesterase enzyme from breaking down acetylcholine, thereby increases levels of the neurotransmitter acetylcholine. Example of an acetyl cholinesterase inhibitor is donepezil. Preferably, the acetylcholinesterase inhibitor is donepezil, or pharmaceutically acceptable salts thereof.

Donepezil is a drug approved for the treatment of mild, moderate, and severe dementia of AD. Donepezil is a reversible inhibitor of the enzyme acetylcholinesterase and sold under trade name Aricept® as hydrochloride salt.

The term, "NMDA receptor antagonist" as used herein refers to class of compounds which act on glutamatergic system by inhibiting the NMDA receptor. Example of NMDA receptor antagonist is memantine. Memantine is a drug approved for treatment of moderate to severe dementia of the AD. Memantine is sold under trade name Namenda® and Namenda XR® as hydrochloride salt.

The phrase, "an effective amount" or "an effective dose" is defined as an amount of a compound of the present invention that (i) treats the particular disease, condition, or disorder, (ii) eliminates one or more symptoms of the particular disease, condition or disorder and (iii) delays the onset of one or more symptoms of the particular disease, condition or disorder described herein.

The term, "pharmaceutically acceptable salt(s)" as used herein refers to salts of the active compound and are prepared by reaction with the appropriate organic or inorganic acids or acid derivatives, depending on the particular substituents found on the compounds described herein. The pharmaceutically acceptable salts include but are not limited to, dimesylate, dihydrochloride, oxalate, succinate, and tartrate and alike. Preferably, the pharmaceutically acceptable salts are dihydrochlorides and dimesylates. More preferably, the pharmaceutically acceptable salt is dimesylate or hydrochloride.

The term, "patient(s)" as used herein refers to an animal. Preferably the term "patient(s)" refers to a mammal. The term mammal includes animals such as mice, rats, dogs, rabbits, pigs, monkeys, elephants, camels, horses, and humans. More preferably the term "patient(s)" refers to humans.

The term "behavioral and psychological symptoms", also known as neuropsychiatric symptoms, refer to a heterogeneous group of non-cognitive symptoms and behaviors occurring in patients with dementia. BPSD constitute a major component of the dementia syndrome irrespective of its subtype. Behavioral and psychological symptoms include domains like agitation/aggression, delusions and/or hallucinations, aberrant motor behavior, aberrant vocalizations, anxiety, elation/euphoria, irritability, depression/dysphoria, apathy, disinhibition, sleep and night time behavior or appetite and eating change. It also refers to any physical or verbal behavior of dementia patients which has the effect of hurting or repelling others, and includes aggressive behaviors such as beating, kicking, biting, and screaming.

The term "dementia" as used herein includes Alzheimer's disease (AD) dementia, dementia associated with schizophrenia, Parkinson's disease (PD) dementia, Lewy body dementia (LBD), vascular dementia, and frontotemporal dementia (FTD).

The term "agitation/aggression" as used herein refers to "agitation and/or aggression".

The term "delusion and/or hallucinations" as used herein refers to "delusions or hallucinations, or delusions and hallucinations.

The term, "Alzheimer's disease" as used herein refers to dementia that causes problems with memory, thinking, and behavior. Alzheimer's disease can be mild, moderate or severe.

The term, "treatment" or "treating" as used herein refers to any treatment of a disease in a mammal, including: (a) slowing or arresting the development of clinical symptoms; and/or (b) causing the regression of clinical symptoms.

In one embodiment, the present invention relates to masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations in a patient with dementia comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to masupirdine, or a pharmaceutically acceptable salt thereof, in combination with memantine, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations in a patient with dementia comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In embodiments, the dementia is selected from Alzheimer's disease dementia, dementia associated with schizophrenia, Parkinson's disease dementia, Lewy body dementia, vascular dementia, and frontotemporal dementia.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with Alzheimer's disease dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia associated with schizophrenia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with Parkinson's disease dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with Lewy body dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with vascular dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with frontotemporal dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with donepezil, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in patients with Alzheimer's disease dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia associated with schizophrenia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with Parkinson's disease dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with Lewy body dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with vascular dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with frontotemporal dementia, comprising administering an effective dose of masupirdine or a pharmaceutically acceptable salt thereof in combination with memantine, or a pharmaceutically acceptable salt thereof.

In some embodiments, the pharmaceutically acceptable salt of masupirdine is selected from mesylate, hydrochloride, oxalate, succinate, and tartrate salts.

In certain embodiments, the pharmaceutically acceptable salt of masupirdine is dimesylate monohydrate.

In embodiments, the pharmaceutically acceptable salt of donepezil is donepezil hydrochloride.

In embodiments, the pharmaceutically acceptable salt of memantine is memantine hydrochloride.

In preferred embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression in a patient with dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating delusions and/or hallucinations in a patient with dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating delusions in a patient with dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating hallucinations in a patient with dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression in a patient with Alzheimer's disease dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating delusions and/or hallucinations in a patient with Alzheimer's disease dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating delusions in a patient with Alzheimer's disease dementia.

In preferred embodiment, the present invention relates to a combination for use in a method of treating hallucinations in a patient with Alzheimer's disease dementia.

In another embodiment, the present invention relates to a combination for use in a method of treating agitation/aggression in a patient with Alzheimer's disease dementia, comprising administering an effective dose of masupirdine dimesylate monohydrate and donepezil hydrochloride or memantine hydrochloride.

In another embodiment, the present invention relates to a combination for use in a method of treating delusions and/or hallucinations in a patient with Alzheimer's disease dementia, comprising administering an effective dose of masupirdine dimesylate monohydrate and donepezil hydrochloride or memantine hydrochloride.

In another embodiment, the present invention relates to a combination for use in a method of treating delusions in a patient with Alzheimer's disease dementia, comprising administering an effective dose of masupirdine dimesylate monohydrate and donepezil hydrochloride or memantine hydrochloride.

In another embodiment, the present invention relates to a combination for use in a method of treating hallucinations in a patient with Alzheimer's disease dementia, comprising administering an effective dose of masupirdine dimesylate monohydrate and donepezil hydrochloride or memantine hydrochloride.

In another embodiment, the present invention relates to a pharmaceutical combination comprising masupirdine dimesylate monohydrate and donepezil hydrochloride.

In another embodiment, the present invention relates to a pharmaceutical combination comprising masupirdine dimesylate monohydrate and memantine hydrochloride.

In another embodiment, the present invention relates to the said pharmaceutical combination for use in the treatment of agitation/aggression in a patient with Alzheimer's disease dementia.

In another embodiment, the present invention relates to the said pharmaceutical combination for use in the treatment of delusions and/or hallucinations in a patient with Alzheimer's disease dementia.

The active ingredients of the present invention can be administered in all possible routes of administration.

The active ingredients of the present invention may be administered by oral, nasal, local, or parenteral routes.

The active ingredients of the present invention can be administered by the same or different route of administration. For instance, the masupirdine of the instant invention can be administered orally and the donepezil or memantine can be administered transdermally.

The active ingredients of the present invention are normally administered by formulating the active ingredients into a pharmaceutical composition in accordance with standard pharmaceutical practice.

The pharmaceutical compositions may be formulated in a conventional manner using one or more pharmaceutically acceptable excipients. The pharmaceutically acceptable excipients are diluents, disintegrants, binders, lubricants, glidants, polymers, coating agents, solvents, co-solvents, preservatives, wetting agents, thickening agents, antifoaming agents, sweetening agents, flavouring agents, antioxidants, colorants, solubilizers, plasticizer, dispersing agents and the like. Excipients are selected from microcrystalline cellulose, mannitol, lactose, pre-gelatinized starch, sodium starch glycolate, corn starch or derivatives thereof, povidone, crospovidone, calcium stearate, glyceryl monostearate, glyceryl palmitostearate, talc, colloidal silicon dioxide, magnesium stearate, sodium lauryl sulfate, sodium stearyl fumarate, zinc stearate, stearic acid or hydrogenated vegetable oil, gum arabica, magnesia, glucose, fats, waxes, natural or hardened oils, water, physiological sodium chloride solution or alcohols, for example, ethanol, propanol or glycerol, sugar solutions, such as glucose solutions or mannitol solutions and the like or a mixture of the various excipients.

The active ingredients of the invention may be formulated in the form of pills, tablets, coated tablets, capsules, powder, granules, pellets, patches, implants, films, semi-solids, liquids, gels, aerosols, emulsions, elixirs and the like. Such pharmaceutical compositions and processes for preparing same are well known in the art.

The pharmaceutical composition may contain <NUM> to <NUM> %, <NUM> to <NUM> % and <NUM> to <NUM> % by weight of the active compounds of the instant invention or pharmaceutically acceptable salt thereof. The amount of the active compounds or its pharmaceutically acceptable salt in the pharmaceutical composition(s) can range from <NUM> to <NUM> or from <NUM> to <NUM> or from <NUM> to <NUM> or from <NUM> to <NUM> or in any range falling within the broader range of <NUM> to <NUM>.

The pharmaceutical composition can be conventional formulations such as immediate release formulations, modified release formulations such as sustained release formulations, delayed release formulations, and extended release formulations or new delivery systems such as orally disintegrating formulations and transdermal patches.

The dose of the active compounds can vary depending on factors such as age and weight of patient, nature, route of administration and severity of the disease to be treated and such other factors.

In yet another aspect, the <NUM>-HT<NUM> receptor antagonist, masupirdine or a pharmaceutically acceptable salt thereof can be administered with donepezil, or a pharmaceutically acceptable salt thereof or memantine, or a pharmaceutically acceptable salt thereof at a daily dose of <NUM> to <NUM>; such as <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM>, preferably at a daily dose of <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM> and most preferably at a daily dose of <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>.

In yet another aspect, donepezil, or a pharmaceutically acceptable salt thereof can be co-administered with masupirdine or a pharmaceutically acceptable salt thereof at a daily dose of <NUM> to <NUM>; <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>, preferably at a daily dose of <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, or <NUM> and most preferably at a daily dose of <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>.

In yet another aspect, donepezil, or a pharmaceutically acceptable salt thereof can be co-administered with masupirdine or a pharmaceutically acceptable salt thereof at a daily dose of <NUM> to <NUM>; such as <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>, preferably at a daily dose of <NUM>, <NUM>, <NUM> or <NUM> and most preferably at a daily dose of <NUM>, <NUM> or <NUM>.

In yet another aspect, memantine, or a pharmaceutically acceptable salt thereof can be co-administered with masupirdine or a pharmaceutically acceptable salt thereof at a daily dose of <NUM> to <NUM>; such as <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>, preferably at a daily dose of <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM>.

In yet another aspect, the treatment comprises administering to the patient <NUM> to <NUM> of masupirdine or a pharmaceutically acceptable salt thereof, per day.

In yet another aspect, the treatment comprises administering to the patient <NUM> to <NUM> of donepezil or a pharmaceutically acceptable salt thereof, per day.

In yet another aspect, the treatment comprises administering to the patient <NUM>, <NUM> or <NUM> of donepezil or a pharmaceutically acceptable salt thereof, per day.

In yet another aspect, the treatment comprises administering to the patient <NUM> to <NUM> of memantine or a pharmaceutically acceptable salt thereof, per day.

In yet another aspect, the treatment comprises administering to the patient <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM>, <NUM> or <NUM> of memantine or a pharmaceutically acceptable salt thereof, per day.

In yet another aspect, the treatment comprises administering the <NUM>-HT<NUM> receptor antagonist, masupirdine or a pharmaceutically acceptable salt thereof and donepezil, or a pharmaceutically acceptable salt thereof or memantine, or a pharmaceutically acceptable salt thereof, to the patient one to three times per day, one to three times per week or one to three times per month. Preferably, the treatment comprises administering the compound to a patient once a day, twice a day or thrice a day. More preferably, the treatment comprises administering the compound to a patient once a day or twice a day.

A stable CHO cell line expressing recombinant human <NUM>-HT<NUM> receptor and pCRE-Luc reporter system was used for cell-based assay. The assay offers a nonradioactive based approach to determine binding of a compound to GPCRs. In this specific assay, the level of intracellular cAMP which is modulated by activation or inhibition of the receptor is measured. The recombinant cells harbor luciferase reporter gene under the control of cAMP response element.

The above cells were grown in <NUM> well clear bottom white plates in Hams F12 medium containing <NUM>% fetal bovine serum (FBS). Prior to the addition of compound or standard agonist, cells were serum starved overnight. Increasing concentrations of test compound were added along with <NUM> of serotonin in OptiMEM medium to the cells. The incubation was continued at <NUM> in CO<NUM> incubator for <NUM>. Medium was removed and cells were washed with phosphate buffered saline. The cells were lysed and luciferase activity was measured in a Lumino meter. Luminescence was plotted against the compound concentrations using GraphPad Prism software. EC<NUM> value of the compound was defined as the concentration required in reducing the luciferase activity by <NUM>%. The Kb value was calculated by feeding the concentration of agonist used in the assay and its EC<NUM> value in the same software.

Masupirdine exhibits antagonistic activity in CRE-Luc based reporter gene assay on the human recombinant <NUM>-HT<NUM> receptor with no detectable agonist activity. The Kb value of masupirdine is <NUM> ± <NUM>.

Compound was tested at MDS pharma services and Novascreen according to the following procedures.

Masupirdine selectively binds to <NUM>-HT<NUM> receptor when tested by the in-vitro radioligand binding technique on the human recombinant <NUM>-HT<NUM> receptor. The Ki value of masupirdine is <NUM>.

Compound was tested according to the following procedures.

Masupirdine binds weakly to <NUM>-HT2A receptors when tested by the in-vitro radioligand binding technique on the human recombinant <NUM>-HT2A receptor. The Ki value of masupirdine is <NUM> ± <NUM>.

Masupirdine was also evaluated for its <NUM>-HT<NUM> receptor selectivity over closely related serotonin subtypes like <NUM>-HT1A, <NUM>-HT1B, <NUM>-HT1D, <NUM>-HT2A, <NUM>-HT2C, <NUM>-HT<NUM>, <NUM>-HT5A, and <NUM>-HT<NUM> in commercial panel at Novascreen.

Masupirdine has shown selectivity of more than <NUM>-fold, over these receptor subtypes.

Male Swiss albino mice of weight range <NUM>-<NUM> (Resident) and <NUM>-<NUM> (Intruder) were used. Resident mice were habituated individually with an ovariectomized female mouse in each cage for at least <NUM> weeks and intruders were habituated socially for at least <NUM> days. β-Estradiol at a dose of <NUM>/kg, s. was administered to ovariectomized female mice during habituation. After habituation for <NUM> weeks, the intruder was exposed to resident mice for a period of <NUM> in the home cage of resident animal and duration of attack was recorded for two days (day <NUM> & <NUM>). During this <NUM> exposure period, ovariectomized female mice were removed from resident cage, resident animals were randomized based on their duration of attack. On day <NUM> (from initial exposure), resident animals were administered respective treatments. Masupirdine (<NUM>/kg, p. ) was administered <NUM> prior to the trial and donepezil (<NUM>/kg, i. ) or memantine (<NUM>/kg, i. ) was administered <NUM> prior to the trial. After post dose interval resident mice were exposed to same intruder for <NUM> and duration of attack was recorded. Data were analyzed using GraphPad Prism software.

Vehicle treated group showed equal duration of attack when compared with its basal. Group treated with masupirdine (<NUM>/kg, p. ) alone showed equal duration of attack when compared with its basal. Group treated with donepezil (<NUM>/kg, i. ) or memantine (<NUM>/kg, i. ) alone showed decrease in duration of attack when compared with its basal; however it was not statistically significant. Group treated with the combination of masupirdine and donepezil or memantine showed significant decrease in duration of attack when compared with its basal (p<<NUM>), demonstrating synergistic effect when administered as combination (<FIG>).

Combined anti-aggressive like activity of masupirdine and donepezil or memantine was better than either alone treatment in resident-intruder task in Swiss albino mice.

DOI induced head twitch response is considered to be a behavioral model of hallucination (<NPL>). Male Wistar rats of <NUM>-<NUM> were weighed and randomized according to their body weights. On day <NUM>, rats were first habituated to the arena for <NUM>. On day <NUM>, masupirdine was administered <NUM> before trial. Donepezil was administered <NUM> before the trial. <NUM> before the trial, rats were subjected to habituation and immediately following the habituation, DOI, <NUM>/kg s. c was administered. The rats were then placed in the arena and head twitches were recorded for <NUM>. Data were analyzed using GraphPad Prism software.

Rats treated with DOI (<NUM>/kg, s. ) showed an increase in head twitches in comparison to the vehicle treated rats. Masupirdine significantly reduced the number of head twitches at a dose of <NUM>/kg, p. Masupirdine in combination with donepezil significantly reduced the number of head twitches in comparison to the standard of care (donepezil) (<FIG>).

Conclusion: Masupirdine in combination with donepezil can attenuate visual hallucinations.

Male Wistar rats (<NUM>-<NUM> body weight) were stereotaxically implanted with a microdialysis guide cannula in prefrontal cortex (PFC; AP: +<NUM>, ML: -<NUM>, DV: -<NUM>) under isoflurane anesthesia. Co-ordinates were taken according to atlas for the rat brain (Paxinos and Watson <NUM>) with reference points taken from bregma and vertical from the skull. The rats were allowed to recover individually for four days in a round bottom Plexiglas bowl with free access to feed and water.

Sixteen hours before start of the study, a pre-equilibrated microdialysis probe (<NUM> dialysis membrane) was inserted into PFC through the guide cannula. On the day of study, probe was perfused with artificial cerebrospinal fluid (aCSF; NaCl <NUM> mmol, KCl <NUM> mmol, MgCl<NUM> <NUM> mmol, CaCl<NUM>. <NUM><NUM>O <NUM> mmol, pH <NUM>) at a flow rate of <NUM>µL/min and a stabilization period of <NUM> was maintained. Four basal samples were collected at <NUM> intervals prior to the treatment of masupirdine (<NUM>/kg, p. ) or vehicle. Donepezil (<NUM>/kg, s. ) or memantine (<NUM>/kg, s. i) or vehicle was administered <NUM> after administration of masupirdine. Dialysate samples were collected for an additional period of <NUM> post treatment of masupirdine (<NUM>/kg, p. Dialysates were stored below -<NUM> prior to analysis.

Norepinephrine and dopamine levels in dialysates were quantified using LC-MS/MS method.

Area under the curve (AUC) values for percent change in neurotransmitter levels were calculated and the statistical significance between the mean AUC value after combination treatment was compared against donepezil treatment using students unpaired t-test. Statistical significance was considered at a p value less than <NUM>.

Treatment with donepezil (<NUM>/kg, s. ) produced increase in cortical norepinephrine levels with AUC value of <NUM> ± <NUM>, whereas combination of masupirdine (<NUM>/kg, p. o) and donepezil (<NUM>/kg, s. ) produced significantly higher AUC (<NUM> ± <NUM>) compared to donepezil alone. These results indicate that the combination produced about <NUM> % higher increase in cortical norepinephrine levels (<FIG>).

Similarly, treatment with donepezil (<NUM>/kg, s. ) produced increase in cortical dopamine levels with AUC value of <NUM> ± <NUM>, whereas combination of masupirdine (<NUM>/kg, p. ) and donepezil (<NUM>/kg, s. ) produced significantly higher AUC (<NUM> ± <NUM>) compared to donepezil alone. Results from this study indicate that the combination produced about <NUM> % higher increase in cortical dopamine levels (<FIG>).

Treatment with memantine (<NUM>/kg, s. ) produced increase in cortical norepinephrine levels with AUC value of <NUM> ± <NUM>, whereas combination of masupirdine (<NUM>/kg, p. ) and memantine (<NUM>/kg, s. ) produced significantly higher AUC values (<NUM> ± <NUM>) compared to memantine alone. These results indicate that combination produced about <NUM>% higher increase in cortical norepinephrine levels (<FIG>).

Similarly, treatment with memantine (<NUM>/kg, s. ) produced increase in cortical dopamine levels with AUC value of <NUM> ± <NUM>, whereas combination of masupirdine (<NUM>/kg, p. ) and memantine (<NUM>/kg, s. ) produced significantly higher AUC values (<NUM> ± <NUM>) compared to memantine alone. Results from this study indicate that combination produced about <NUM>% higher increase in cortical dopamine levels (<FIG>).

Claim 1:
Masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil or memantine, or a pharmaceutically acceptable salt thereof, for use in a method of treating agitation/aggression, delusions, hallucinations, or delusions and hallucinations, in a patient with dementia, comprising administering an effective dose of masupirdine, or a pharmaceutically acceptable salt thereof, in combination with donepezil or memantine, or a pharmaceutically acceptable salt thereof.