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Dataset Card

Dataset Summary

The dataset contains fluorescence microscopy images of cells stained with DAPI (Which stains the nucleus, channel 1) and CyclinB1 (which stains cytoplasm and nucleus, if it stains the nucleus it indicates the cell is in G2 or early prophase, channel 3). The goal of this project is to analyze whether Cyclin B1 is in the nucleus to determine where the cell is in the cell cycle.

Each cell has two corresponding TIFF images:

  • A DAPI image showing the nucleus
  • A CyclinB1 image showing cell cycle protein levels

The dataset was created for OPALS Group 19 project focused on understanding how CyclinB1 shows where the cell is in the cell cycle.

Data Fields

  • image — A PIL Image
  • source — The source of the data

Data Collection

Images were collected using fluorescence microscopy.
Cells were stained with:

  • DAPI to visualize nuclei
  • CyclinB1 to measure cell cycle progression

The dataset was prepared manually by organizing images and creating a JSON metadata file linking each cell’s channels.


Intended Uses

This dataset can be used for:

  • Cell segmentation
  • Cell cycle classification
  • Training or testing microscopy image models
  • Educational demonstrations of fluorescence imaging

Limitations

  • Small dataset size (fewer than 1,000 samples)
  • Only two fluorescence channels
  • No segmentation masks included
  • Images may vary slightly in brightness or focus

Citation

If you use this dataset, please cite the dataset creator or link to the Hugging Face dataset page.

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