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Parse 100K PBMC Cytokines (IFN-γ vs PBS)
Balanced 100,000-cell subsample of the ~10 million cell cytokine stimulation dataset from Parse Biosciences.
In the original experiment, peripheral blood mononuclear cells (PBMCs) from twelve healthy donors were treated with either one of 90 different cytokines or a phosphate-buffered saline (PBS) control for 24 hours, yielding (90 + 1) × 12 = 1,092 experimental conditions.
For the brisc tutorials, the data is restricted to the interferon-gamma (IFN-γ)
versus PBS contrast and subsampled to 50,000 cells per condition, stratified by
cell type. The result is 100,000 cells × 40,352 genes of raw counts across 24
samples (12 donors × 2 conditions) and 18 annotated cell types, with obs
columns sample, donor, cell_type, treatment, and cytokine.
Source
- https://www.parsebiosciences.com/datasets/10-million-human-pbmcs-in-a-single-experiment/
- https://www.biorxiv.org/content/10.64898/2025.12.12.693897v1
All credit to Parse Biosciences.
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