# EDGAR Filing Document

**Accession Number:** 0001864290
**File Stem:** 0001752724-23-063466
**Filing Date:** 2023-3
**Character Count:** 51038
**Document Hash:** 0c59f38b58bcb8521d9d672183548651
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001752724-23-063466.hdr.sgml**: 20230322

**ACCESSION NUMBER**: 0001752724-23-063466

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 2

**CONFORMED PERIOD OF REPORT**: 20230131

**FILED AS OF DATE**: 20230322

**DATE AS OF CHANGE**: 20230322

**PERIOD START**: 20230430

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Pioneer Municipal High Income Opportunities Fund, Inc.
- **CENTRAL INDEX KEY:** 0001864290
- **IRS NUMBER:** 000000000
- **STATE OF INCORPORATION:** MD
- **FISCAL YEAR END:** 0430

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-23699
- **FILM NUMBER:** 23752364

**BUSINESS ADDRESS:**
- **STREET 1:** 60 STATE STREET
- **STREET 2:** 5TH FLOOR
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02109
- **BUSINESS PHONE:** 617-742-7825

**MAIL ADDRESS:**
- **STREET 1:** 60 STATE STREET
- **STREET 2:** 5TH FLOOR
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02109

## Nport-Ex

## Pioneer Municipal High Income Opportunities Fund, Inc.
Schedule of Investments \| January 31, 2023

&nbsp;&nbsp;**Ticker Symbol: MIO** 

------

### Schedule of Investments \| 1/31/23
(unaudited)

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;**Principal<br> Amount<br> USD ($)** |  | **Value** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**UNAFFILIATED ISSUERS — 140.8%** |  |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Commercial Mortgage-Backed<br> Security—0.5% of Net Assets** |  |
| 1500000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Housing Opportunities Commission of Montgomery County, Series 2021-A, 2.10%, 7/1/52 | $986880 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Commercial Mortgage-Backed Security<br> (Cost $1,240,572)** | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **$** **986880** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Municipal Bonds — 140.3% of Net Assets(a)** |  |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Alabama — 7.4%** |  |
| 18265000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Tuscaloosa County Industrial Development Authority, Hunt Refining Project, Series A, 5.25%, 5/1/44 (144A) | $16449094 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Alabama** | &nbsp;&nbsp; **$16449094** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Arizona — 7.0%** |  |
| 1000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Arizona Industrial Development Authority, Series A, 4.00%, 7/15/51 (144A) | $774670 |
| 950000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Arizona Industrial Development Authority, Series A, 4.00%, 7/15/56 (144A) | &nbsp;&nbsp; 717573 |
| 1675000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Arizona Industrial Development Authority, Doral Academy Nevada Fire Mesa, Series A, 5.00%, 7/15/49 (144A) | &nbsp;&nbsp; 1547432 |
| 8000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Maricopa County Industrial Development Authority, Commercial Metals Company, 4.00%, 10/15/47 (144A) | &nbsp;&nbsp; 6985280 |
| 7040000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Maricopa County Industrial Development Authority, Honorhealth, Series A, 3.00%, 9/1/51 | &nbsp;&nbsp; 5378349 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Arizona** | &nbsp;&nbsp; **$15403304** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Arkansas — 3.8%** |  |
| 9500000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Arkansas Development Finance Authority, Big River Steel Project, 4.50%, 9/1/49 (144A) | $8505920 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Arkansas** | &nbsp;&nbsp;&nbsp;&nbsp; **$8505920** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**California — 20.0%** |  |
| 1000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;California County Tobacco Securitization Agency, Golden Gate Tobacco Settlement, Series A, 5.00%, 6/1/47 | $941110 |
| 1000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;California Statewide Communities Development Authority, Baptist University, Series A, 5.00%, 11/1/41 (144A) | &nbsp;&nbsp; 1006740 |
| 1500000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;California Statewide Communities Development Authority, Loma Linda University Medical Center, Series A, 5.00%, 12/1/46 (144A) | &nbsp;&nbsp; 1463145 |

---

1Pioneer Municipal High Income Opportunities Fund, Inc. \| \| 1/31/23

------

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;**Principal<br> Amount<br> USD ($)** |  | **Value** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**California — (continued)** |  |
| 13315000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;California Statewide Communities Development Authority, Loma Linda University Medical Center, Series A, 5.25%, 12/1/56 (144A) | $13399950 |
| 750000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City of Oroville, Oroville Hospital, 5.25%, 4/1/39 | &nbsp;&nbsp; 772373 |
| 3865000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City of Oroville, Oroville Hospital, 5.25%, 4/1/49 | &nbsp;&nbsp; 3893021 |
| 8550000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City of Oroville, Oroville Hospital, 5.25%, 4/1/54 | &nbsp;&nbsp; 8567869 |
| 17000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Golden State Tobacco Securitization Corp., Series A1, 4.214%, 6/1/50 | &nbsp;&nbsp; 14098270 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total California** | &nbsp;&nbsp; **$44142478** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Colorado — 7.6%** |  |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Aerotropolis Regional Transportation Authority, 4.375%, 12/1/52 | $1664960 |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City & County of Denver, United Airlines, Inc. Project, 5.00%, 10/1/32 | &nbsp;&nbsp; 2005000 |
| 1,900,000(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cottonwood Highlands Metropolitan District No. 1, Series A, 5.00%, 12/1/49 | &nbsp;&nbsp; 1780072 |
| 7000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Dominion Water & Sanitation District, 5.875%, 12/1/52 | &nbsp;&nbsp; 7116480 |
| 3475000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Prairie Center Metropolitan District No 3, Series A, 5.00%, 12/15/41 (144A) | &nbsp;&nbsp; 3481637 |
| 1000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Rampart Range Metropolitan District No 5, 4.00%, 12/1/51 | &nbsp;&nbsp; 712360 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Colorado** | &nbsp;&nbsp; **$16760509** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Delaware — 0.4%** |  |
| 25000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Delaware State Economic Development Authority, Aspira of Delaware Charter, 4.00%, 6/1/52 | $19673 |
| 1015000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Delaware State Economic Development Authority, Aspira of Delaware Charter, 4.00%, 6/1/57 | &nbsp;&nbsp; 776617 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Delaware** | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **$796290** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Florida — 5.0%** |  |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Charlotte County Industrial Development Authority, Town & Country Utilities, Series B, 5.00%, 10/1/36 (144A) | $1730280 |
| 2425000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Florida Development Finance Corp., Glenridge On Palmer Ranch Project, 5.00%, 6/1/51 | &nbsp;&nbsp; 2007924 |
| 2450000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;South Broward Hospital District, Series A, 2.375%, 5/1/45 | &nbsp;&nbsp; 1687658 |
| 2455000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;South Broward Hospital District, Series A, 2.50%, 5/1/47 | &nbsp;&nbsp; 1689703 |

---

Pioneer Municipal High Income Opportunities Fund, Inc. \| \| 1/31/232

------

(unaudited) (continued)

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;**Principal<br> Amount<br> USD ($)** |  | **Value** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Florida — (continued)** |  |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;South Broward Hospital District, Series A, 3.00%, 5/1/51 | $1539020 |
| 3700000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;State of Florida Department of Transportation Turnpike System Revenue, Series B, 2.125%, 7/1/46 | &nbsp;&nbsp; 2324414 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Florida** | &nbsp;&nbsp; **$10978999** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Georgia — 2.6%** |  |
| 8,440,000(c) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Tender Option Bond Trust Receipts/Certificates, Series 2021, 2.884%, 7/15/50 (ST GTD) (144A) | $5848896 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Georgia** | &nbsp;&nbsp;&nbsp;&nbsp; **$5848896** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Illinois — 11.6%** |  |
| 3,050,000(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Chicago Board of Education, Series A, 5.00%, 12/1/35 | $3154859 |
| 4,260,000(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Chicago Board of Education, Series A, 5.00%, 12/1/42 | &nbsp;&nbsp; 4207687 |
| 2,255,000(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City of Chicago, Series A, 5.50%, 1/1/33 | &nbsp;&nbsp; 2293042 |
| 9500000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Metropolitan Pier & Exposition Authority, McCormick Place Expansion, 4.00%, 6/15/50 | &nbsp;&nbsp; 8523305 |
| 7230000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Metropolitan Pier & Exposition Authority, McCormick Place Expansion, 5.00%, 6/15/50 | &nbsp;&nbsp; 7348283 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Illinois** | &nbsp;&nbsp; **$25527176** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Iowa — 0.4%** |  |
| 1000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Iowa Finance Authority, Alcoa Inc. Projects, 4.75%, 8/1/42 | $989170 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Iowa** | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **$989170** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Minnesota — 0.6%** |  |
| 1430000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City of Rochester, Rochester Math & Science Academy, Series A, 5.125%, 9/1/38 | $1404861 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Minnesota** | &nbsp;&nbsp;&nbsp;&nbsp; **$1404861** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**New Jersey — 1.5%** |  |
| 1715000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;New Jersey Health Care Facilities Financing Authority, Atlanticare Health System, 3.00%, 7/1/51 | $1270335 |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Tobacco Settlement Financing Corp., Series B, 5.00%, 6/1/46 | &nbsp;&nbsp; 2018360 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total New Jersey** | &nbsp;&nbsp;&nbsp;&nbsp; **$3288695** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**New York — 19.3%** |  |
| 4300000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Chautauqua Tobacco Asset Securitization Corp., 5.00%, 6/1/48 | $4215290 |
| 4385000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Erie Tobacco Asset Securitization Corp., Asset-Backed, Series A, 5.00%, 6/1/45 | &nbsp;&nbsp; 4342378 |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Metropolitan Transportation Authority, Green Bond, Series C-1, 4.75%, 11/15/45 | &nbsp;&nbsp; 1998680 |

---

3Pioneer Municipal High Income Opportunities Fund, Inc. \| \| 1/31/23

------

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;**Principal<br> Amount<br> USD ($)** |  | **Value** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**New York — (continued)** |  |
| 3505000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nassau County Tobacco Settlement Corp., Asset-Backed, Series A3, 5.00%, 6/1/35 | $3331292 |
| 2895000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Nassau County Tobacco Settlement Corp., Asset-Backed, Series A3, 5.125%, 6/1/46 | &nbsp;&nbsp; 2724108 |
| 7030000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;New York Counties Tobacco Trust IV, Series A, 5.00%, 6/1/42 | &nbsp;&nbsp; 6606302 |
| 7915000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;New York Counties Tobacco Trust IV, Series A, 5.00%, 6/1/45 | &nbsp;&nbsp; 7370606 |
| 8325000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;TSASC, Inc., Series B, 5.00%, 6/1/48 | &nbsp;&nbsp; 7825500 |
| 6000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Westchester County Local Development Corp., Purchase Senior Learning Community, 4.50%, 7/1/56 (144A) | &nbsp;&nbsp; 4240500 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total New York** | &nbsp;&nbsp; **$42654656** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**North Carolina — 3.2%** |  |
| 5000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;County of Johnston NC Water & Sewer System Revenue, 2.25%, 4/1/51 | $3030400 |
| 4000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;North Carolina Department of Transportation, I-77 Hot Lanes Project, 5.00%, 6/30/54 | &nbsp;&nbsp; 4016600 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total North Carolina** | &nbsp;&nbsp;&nbsp;&nbsp; **$7047000** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Ohio — 8.0%** |  |
| 18590000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Buckeye Tobacco Settlement Financing Authority, Senior Class 2, Series B2, 5.00%, 6/1/55 | $17702699 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Ohio** | &nbsp;&nbsp; **$17702699** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Oklahoma — 2.7%** |  |
| 5900000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Tulsa Airports Improvement Trust, Series C, 5.50%, 12/1/35 | $5917641 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Oklahoma** | &nbsp;&nbsp;&nbsp;&nbsp; **$5917641** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Pennsylvania — 0.7%** |  |
| 1750000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Pennsylvania Economic Development Financing Authority, UPMC, Series A, 4.00%, 10/15/51 | $1640783 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Pennsylvania** | &nbsp;&nbsp;&nbsp;&nbsp; **$1640783** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Puerto Rico — 24.1%** |  |
| 18,673,960(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Commonwealth of Puerto Rico, Restructured Series A1, 4.00%, 7/1/37 | $16594241 |
| 1,796,000(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Commonwealth of Puerto Rico, Restructured Series A1, 4.00%, 7/1/41 | &nbsp;&nbsp; 1542495 |
| 3,624,000(b) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Commonwealth of Puerto Rico, Restructured Series A1, 4.00%, 7/1/46 | &nbsp;&nbsp; 3021727 |
| 3220000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Commonwealth Aqueduct & Sewer Authority, Series A, 5.00%, 7/1/37 (144A) | &nbsp;&nbsp; 3264951 |

---

Pioneer Municipal High Income Opportunities Fund, Inc. \| \| 1/31/234

------

(unaudited) (continued)

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;**Principal<br> Amount<br> USD ($)** |  | **Value** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Puerto Rico — (continued)** |  |
| 8500000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Commonwealth Aqueduct & Sewer Authority, Series A, 5.00%, 7/1/47 (144A) | $8346745 |
| 2435000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Commonwealth Aqueduct & Sewer Authority, Series B, 5.00%, 7/1/37 (144A) | &nbsp;&nbsp; 2471647 |
| 2720000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Electric Power Authority, Series Class V, 5.25%, 7/1/27 (AGM Insured) | &nbsp;&nbsp; 2775488 |
| 2000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Highway & Transportation Authority, Restructured Series A, 5.00%, 7/1/62 | &nbsp;&nbsp; 1925000 |
| 12844000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Sales Tax Financing Corp. Sales Tax Revenue, Restructured Series A1, 4.75%, 7/1/53 | &nbsp;&nbsp; 12205011 |
| 1000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Puerto Rico Sales Tax Financing Corp. Sales Tax Revenue, Restructured Series A1, 5.00%, 7/1/58 | &nbsp;&nbsp; 985250 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Puerto Rico** | &nbsp;&nbsp; **$53132555** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Texas — 4.6%** |  |
| 11510000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;City of Houston Airport System Revenue, 4.00%, 7/15/41 | $10237569 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Texas** | &nbsp;&nbsp; **$10237569** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Virginia — 5.4%** |  |
| 7480000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Tobacco Settlement Financing Corp., Series A1, 6.706%, 6/1/46 | $7072265 |
| 4750000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Virginia Small Business Financing Authority, Transform 66 P3 Project, 5.00%, 12/31/52 | &nbsp;&nbsp; 4785768 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Virginia** | &nbsp;&nbsp; **$11858033** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Wisconsin — 4.4%** |  |
| 8000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Public Finance Authority, Searstone CCRC Project, 4.00%, 6/1/41 (144A) | $6085040 |
| 5000000 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Wisconsin Health & Educational Facilities Authority, Aspirus, Inc. Obligated Group, 3.00%, 8/15/51 | &nbsp;&nbsp; 3618650 |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Wisconsin** | &nbsp;&nbsp;&nbsp;&nbsp; **$9703690** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**Total Municipal Bonds<br> (Cost $320,018,573)** | **$309990018** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**TOTAL INVESTMENTS IN UNAFFILIATED ISSUERS — 140.8%<br> (Cost $321,259,145)** | **$310976898** |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**OTHER ASSETS AND LIABILITIES — (40.8)%** | $(90101331) |
|  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**net assets — 100.0%** | **$220875567** |

---

5Pioneer Municipal High Income Opportunities Fund, Inc. \| \| 1/31/23

------

---

| | |
|:---|:---|
| AGM | &nbsp;&nbsp;Assured Guaranty Municipal Corp. |
| ST GTD | &nbsp;&nbsp;State Guaranteed. |
| (144A) | &nbsp;&nbsp;Security is exempt from registration under Rule 144A of the Securities Act of 1933. Such securities may be resold normally to qualified institutional buyers in a transaction exempt from registration. At January 31, 2023, the value of these securities amounted to $86,319,500, or 39.1% of net assets. |

---

(a) Consists of Revenue Bonds unless otherwise indicated.

(b) Represents a General Obligation Bond.

(c) The interest rate is subject to change periodically. The interest rate and/or reference index and spread shown at January 31, 2023.

Various inputs are used in determining the value of the Fund's investments. These inputs are summarized in the three broad levels below.

---

| |
|:---|
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Level 1 – unadjusted quoted prices in active markets for identical securities. |
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Level 2 – other significant observable inputs (including quoted prices for similar securities, interest rates, prepayment speeds, credit risks, etc.). |
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Level 3 – significant unobservable inputs (including the Adviser's own assumptions in determining fair value of investments). |

---

The following is a summary of the inputs used as of January 31, 2023, in valuing the Fund's investments:

---

| | | | | |
|:---|:---|:---|:---|:---|
|  | **Level 1** | &nbsp;&nbsp;**Level 2** | &nbsp;&nbsp;**Level 3** | &nbsp;&nbsp;**Total** |
| Commercial Mortgage-Backed Security | $— | &nbsp;&nbsp;&nbsp;&nbsp;$986880 | &nbsp;&nbsp;$— | &nbsp;&nbsp;&nbsp;&nbsp;$986880 |
| Municipal Bonds | &nbsp;&nbsp;&nbsp;— | &nbsp;&nbsp;&nbsp;309990018 | &nbsp;&nbsp;&nbsp;— | &nbsp;&nbsp;&nbsp;309990018 |
| **Total Investments in Securities** | **$—** | &nbsp;&nbsp;**$310976898** | &nbsp;&nbsp;**$—** | &nbsp;&nbsp;**$310976898** |

---

During the period ended January 31, 2023, there were no transfers in or out of Level 3.

Pioneer Municipal High Income Opportunities Fund, Inc. \| \| 1/31/236

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** Pioneer Municipal High Income Opportunities Fund, Inc.

- **b. Investment Company Act file number:** 811-23699

- **c. CIK number of Registrant:** 0001864290

- **d. LEI of Registrant:** 5493005BKX8X756MKL25

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 60 State Street

  - **City:** Boston

  - **State:** MA

  - **Foreign country:** US

  - **Zip / Postal Code:** 02109

  - **Telephone number:** 617-422-4947

**Item A.2. Information about the Series.**

- **a. Name of Series:** Pioneer Municipal High Income Opportunities Fund, Inc.

- **c. LEI of Series:** 5493005BKX8X756MKL25

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2023-04-30

- **b. Date as of which information is reported:** 2023-01-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $313777842.68

**Total Liabilities:** $92902275.42

**Net Assets:** $220875567.26

**Currency Risk Metrics (dv01):**

- 

**Credit Spread Risk - Investment Grade (dv01):**

- **3-Month:** 0.00000000 | **1-Year:** 0.00000000 | **5-Year:** -15066.00000000 | **10-Year:** 0.00000000 | **30-Year:** -61067.00000000

**Credit Spread Risk - Non-Investment Grade (dv01):**

- **3-Month:** -598.00000000 | **1-Year:** -1437.00000000 | **5-Year:** -19538.00000000 | **10-Year:** -7631.00000000 | **30-Year:** -147612.00000000

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000000000 | 11.99%               | -0.67%               | 7.00%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $-167249.26              | $22100018.61                               |
| Month 2  | $-15717560.87            | $13610414.12                               |
| Month 3  | $-7916314.67             | $21519026.93                               |

### Schedule of Portfolio Investments

| Name                           | Title                                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| PUBLIC FIN AUTH WI REVENUE     | Public Finance Authority                                              | CUSIP: 74442PTK1<br>LEI: 5493004S6D3NA627K012 | Long             | DBT              | MUN               | US        |   8000000 | PA      | $6085040.00   | 2.75%             | 2041-06-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| NEW YORK CNTYS NY TOBACCO TRUS | New York Counties Tobacco Trust IV                                    | CUSIP: 62947YAD3<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   7030000 | PA      | $6606301.90   | 2.99%             | 2042-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| SOUTH BROWARD FL HOSP DIST     | South Broward Hospital District                                       | CUSIP: 836753NM1<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2000000 | PA      | $1539020.00   | 0.70%             | 2051-05-01      | Fixed         | 3.00%                 | No            |                  2 | On Loan: No      |
| TOBACCO SETTLEMENT FING CORP N | Tobacco Settlement Financing Corp                                     | CUSIP: 888808HT2<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2000000 | PA      | $2018360.00   | 0.91%             | 2046-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| DOMINION WTR & SANTN DIST CO R | Dominion Water & Sanitation District                                  | CUSIP: 25755AAE1<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   7000000 | PA      | $7116480.00   | 3.22%             | 2052-12-01      | Fixed         | 5.88%                 | No            |                  2 | On Loan: No      |
| OROVILLE CA REVENUE            | City of Oroville CA                                                   | CUSIP: 687241AK5<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |    750000 | PA      | $772372.50    | 0.35%             | 2039-04-01      | Fixed         | 5.25%                 | No            |                  2 | On Loan: No      |
| MET PIER & EXPOSITION AUTH IL  | Metropolitan Pier & Exposition Authority                              | CUSIP: 592250DM3<br>LEI: 254900I3VDCQ58UGBR15 | Long             | DBT              | MUN               | US        |   7230000 | PA      | $7348282.80   | 3.33%             | 2050-06-15      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| CHAUTAUQUA NY TOBACCO ASSET SE | Chautauqua Tobacco Asset Securitization Corp                          | CUSIP: 162564BB3<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   4300000 | PA      | $4215290.00   | 1.91%             | 2048-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH AQUEDUCT &  | Puerto Rico Commonwealth Aqueduct & Sewer Authority                   | CUSIP: 745160UD1<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   3220000 | PA      | $3264951.20   | 1.48%             | 2037-07-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| NASSAU CNTY NY TOBACCO SETTLEM | Nassau County Tobacco Settlement Corp                                 | CUSIP: 63166MCA5<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   3505000 | PA      | $3331292.20   | 1.51%             | 2035-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO ELEC PWR AUTH PWR  | Puerto Rico Electric Power Authority                                  | CUSIP: 74526QPL0<br>LEI: 5493003BRB67HF8ST418 | Long             | DBT              | MUN               | US        |   2720000 | PA      | $2775488.00   | 1.26%             | 2027-07-01      | Fixed         | 5.25%                 | No            |                  2 | On Loan: No      |
| CHICAGO IL BRD OF EDU          | Chicago Board of Education                                            | CUSIP: 167505XL5<br>LEI: 54930099NR6T9V7LVB43 | Long             | DBT              | MUN               | US        |   3050000 | PA      | $3154859.00   | 1.43%             | 2035-12-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| MET TRANSPRTN AUTH NY REVENUE  | Metropolitan Transportation Authority                                 | CUSIP: 59261AG35<br>LEI: 5493000TKYODLXADQD60 | Long             | DBT              | MUN               | US        |   2000000 | PA      | $1998680.00   | 0.90%             | 2045-11-15      | Fixed         | 4.75%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH AQUEDUCT &S | Puerto Rico Commonwealth Aqueduct & Sewer Authority                   | CUSIP: 745160TG6<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2435000 | PA      | $2471646.75   | 1.12%             | 2037-07-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| CALIFORNIA STWD CMNTYS DEV AUT | California Statewide Communities Development Authority                | CUSIP: 13080SSJ4<br>LEI: 549300KTNI2GCJNX2U48 | Long             | DBT              | MUN               | US        |   1000000 | PA      | $1006740.00   | 0.46%             | 2041-11-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| PRAIRIE CENTER CO MET DIST #3  | Prairie Center Metropolitan District No 3                             | CUSIP: 739476AE9<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   3475000 | PA      | $3481637.25   | 1.58%             | 2041-12-15      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| OROVILLE CA REVENUE            | City of Oroville CA                                                   | CUSIP: 687241AL3<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   3865000 | PA      | $3893021.25   | 1.76%             | 2049-04-01      | Fixed         | 5.25%                 | No            |                  2 | On Loan: No      |
| OROVILLE CA REVENUE            | City of Oroville CA                                                   | CUSIP: 687241AM1<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   8550000 | PA      | $8567869.50   | 3.88%             | 2054-04-01      | Fixed         | 5.25%                 | No            |                  2 | On Loan: No      |
| NEW JERSEY ST HLTH CARE FACS F | New Jersey Health Care Facilities Financing Authority                 | CUSIP: 645790PE0<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1715000 | PA      | $1270334.80   | 0.58%             | 2051-07-01      | Fixed         | 3.00%                 | No            |                  2 | On Loan: No      |
| CALIFORNIA STWD CMNTYS DEV AUT | California Statewide Communities Development Authority                | CUSIP: 13080SMK7<br>LEI: 549300KTNI2GCJNX2U48 | Long             | DBT              | MUN               | US        |   1500000 | PA      | $1463145.00   | 0.66%             | 2046-12-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| TENDER OPTION BOND TRUST RECEI | Tender Option Bond Trust Receipts/Certificates                        | CUSIP: 88034YC32<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   8440000 | PA      | $5848896.45   | 2.65%             | 2050-07-15      | Variable      | 0.01%                 | No            |                  2 | On Loan: No      |
| ARIZONA ST INDL DEV AUTH EDU R | Arizona Industrial Development Authority                              | CUSIP: 04052BHZ0<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1675000 | PA      | $1547432.00   | 0.70%             | 2049-07-15      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| TUSCALOOSA CNTY AL INDL DEV AU | Tuscaloosa County Industrial Development Authority                    | CUSIP: 90068FAZ9<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |  18265000 | PA      | $16449093.70  | 7.45%             | 2044-05-01      | Fixed         | 5.25%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO SALES TAX FING COR | Puerto Rico Sales Tax Financing Corp Sales Tax Revenue                | CUSIP: 74529JPX7<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1000000 | PA      | $985250.00    | 0.45%             | 2058-07-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| ARIZONA ST INDL DEV AUTH EDU R | Arizona Industrial Development Authority                              | CUSIP: 04052BPT5<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |    950000 | PA      | $717573.00    | 0.32%             | 2056-07-15      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| NASSAU CNTY NY TOBACCO SETTLEM | Nassau County Tobacco Settlement Corp                                 | CUSIP: 63166MCB3<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2895000 | PA      | $2724108.15   | 1.23%             | 2046-06-01      | Fixed         | 5.12%                 | No            |                  2 | On Loan: No      |
| SOUTH BROWARD FL HOSP DIST     | South Broward Hospital District                                       | CUSIP: 836753NK5<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2455000 | PA      | $1689702.85   | 0.77%             | 2047-05-01      | Fixed         | 2.50%                 | No            |                  2 | On Loan: No      |
| NORTH CAROLINA DEPT TRANSN     | North Carolina Department of Transportation                           | CUSIP: 65820FAF4<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   4000000 | PA      | $4016600.00   | 1.82%             | 2054-06-30      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| PENNSYLVANIA ST ECON DEV FING  | Pennsylvania Economic Development Financing Authority                 | CUSIP: 70870JDJ4<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1750000 | PA      | $1640782.50   | 0.74%             | 2051-10-15      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| ARIZONA ST INDL DEV AUTH EDU R | Arizona Industrial Development Authority                              | CUSIP: 04052BPS7<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1000000 | PA      | $774670.00    | 0.35%             | 2051-07-15      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| TOBACCO SETTLEMENT FING CORP V | Tobacco Settlement Financing Corp/VA                                  | CUSIP: 88880NAT6<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   7480000 | PA      | $7072265.20   | 3.20%             | 2046-06-01      | Fixed         | 6.71%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH AQUEDUCT &  | Puerto Rico Commonwealth Aqueduct & Sewer Authority                   | CUSIP: 745160SJ1<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   8500000 | PA      | $8346745.00   | 3.78%             | 2047-07-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH             | Commonwealth of Puerto Rico                                           | CUSIP: 74514L3P0<br>LEI: 549300X44HXPLJU48U63 | Long             | DBT              | MUN               | US        |   3624000 | PA      | $3021727.44   | 1.37%             | 2046-07-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| IOWA ST FIN AUTH MIDWSTRN DISA | Iowa Finance Authority                                                | CUSIP: 46246SAE5<br>LEI: 549300NQVL49BTHOW391 | Long             | DBT              | MUN               | US        |   1000000 | PA      | $989170.00    | 0.45%             | 2042-08-01      | Fixed         | 4.75%                 | No            |                  2 | On Loan: No      |
| HOUSTON TX ARPT SYS REVENUE    | City of Houston TX Airport System Revenue                             | CUSIP: 442349GA8<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |  11510000 | PA      | $10237569.50  | 4.63%             | 2041-07-15      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| VIRGINIA SMALL BUSINESS FING A | Virginia Small Business Financing Authority                           | CUSIP: 928097AC8<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   4750000 | PA      | $4785767.50   | 2.17%             | 2052-12-31      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| CALIFORNIA CNTY CA TOBACCO SEC | California County Tobacco Securitization Agency                       | CUSIP: 13016NDF8<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1000000 | PA      | $941110.00    | 0.43%             | 2047-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| COTTONWOOD HIGHLANDS MET DIST  | Cottonwood Highlands Metropolitan District No 1                       | CUSIP: 221865AA6<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1900000 | PA      | $1780072.00   | 0.81%             | 2049-12-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| CALIFORNIA STWD CMNTYS DEV AUT | California Statewide Communities Development Authority                | CUSIP: 13080SML5<br>LEI: 549300KTNI2GCJNX2U48 | Long             | DBT              | MUN               | US        |  13315000 | PA      | $13399949.70  | 6.07%             | 2056-12-01      | Fixed         | 5.25%                 | No            |                  2 | On Loan: No      |
| DELAWARE ST ECON DEV AUTH CHRT | Delaware State Economic Development Authority                         | CUSIP: 246372BD7<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1015000 | PA      | $776617.10    | 0.35%             | 2057-06-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| DELAWARE ST ECON DEV AUTH CHRT | Delaware State Economic Development Authority                         | CUSIP: 246372BC9<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |     25000 | PA      | $19672.75     | 0.01%             | 2052-06-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| CHARLOTTE CNTY FL INDL DEV AUT | Charlotte County Industrial Development Authority/FL                  | CUSIP: 16080TAH8<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2000000 | PA      | $1730280.00   | 0.78%             | 2036-10-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| CHICAGO IL                     | City of Chicago IL                                                    | CUSIP: 167486WN5<br>LEI: T5UYO5SYLC8K73MJQX30 | Long             | DBT              | MUN               | US        |   2255000 | PA      | $2293041.85   | 1.04%             | 2033-01-01      | Fixed         | 5.50%                 | No            |                  2 | On Loan: No      |
| MARICOPA CNTY INDL DEV AUTH EX | Maricopa County Industrial Development Authority                      | CUSIP: 56678TAA7<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   8000000 | PA      | $6985280.00   | 3.16%             | 2047-10-15      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| WISCONSIN ST HLTH & EDUCTNL FA | Wisconsin Health & Educational Facilities Authority                   | CUSIP: 97712JDN0<br>LEI: 549300ISNGXS0VHGVD41 | Long             | DBT              | MUN               | US        |   5000000 | PA      | $3618650.00   | 1.64%             | 2051-08-15      | Fixed         | 3.00%                 | No            |                  2 | On Loan: No      |
| ARKANSAS ST DEV FIN AUTH INDL  | Arkansas Development Finance Authority                                | CUSIP: 04108WCC4<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   9500000 | PA      | $8505920.00   | 3.85%             | 2049-09-01      | Fixed         | 4.50%                 | No            |                  2 | On Loan: No      |
| GOLDEN ST TOBACCO SECURITIZATI | Golden State Tobacco Securitization Corp                              | CUSIP: 38122ND66<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |  17000000 | PA      | $14098270.00  | 6.38%             | 2050-06-01      | Fixed         | 4.21%                 | No            |                  2 | On Loan: No      |
| MET PIER & EXPOSITION AUTH IL  | Metropolitan Pier & Exposition Authority                              | CUSIP: 592250DN1<br>LEI: 254900I3VDCQ58UGBR15 | Long             | DBT              | MUN               | US        |   9500000 | PA      | $8523305.00   | 3.86%             | 2050-06-15      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| SOUTH BROWARD FL HOSP DIST     | South Broward Hospital District                                       | CUSIP: 836753NH2<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2450000 | PA      | $1687658.00   | 0.76%             | 2045-05-01      | Fixed         | 2.38%                 | No            |                  2 | On Loan: No      |
| MARICOPA CNTY AZ INDL DEV AUTH | Maricopa County Industrial Development Authority                      | CUSIP: 56678PBL0<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   7040000 | PA      | $5378348.80   | 2.44%             | 2051-09-01      | Fixed         | 3.00%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO SALES TAX FING COR | Puerto Rico Sales Tax Financing Corp Sales Tax Revenue                | CUSIP: 74529JPW9<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |  12844000 | PA      | $12205011.00  | 5.53%             | 2053-07-01      | Fixed         | 4.75%                 | No            |                  2 | On Loan: No      |
| ROCHESTER MN                   | City of Rochester MN                                                  | CUSIP: 77158CAB7<br>LEI: 549300006EX3INSHXA95 | Long             | DBT              | MUN               | US        |   1430000 | PA      | $1404860.60   | 0.64%             | 2038-09-01      | Fixed         | 5.12%                 | No            |                  2 | On Loan: No      |
| FLORIDA ST DEV FIN CORP SENIOR | Florida Development Finance Corp                                      | CUSIP: 34061WAY0<br>LEI: 549300HC854CNBLMWC48 | Long             | DBT              | MUN               | US        |   2425000 | PA      | $2007924.25   | 0.91%             | 2051-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| AEROTROPOLIS REGL TRANPRTN AUT | Aerotropolis Regional Transportation Authority                        | CUSIP: 008051AC8<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   2000000 | PA      | $1664960.00   | 0.75%             | 2052-12-01      | Fixed         | 4.38%                 | No            |                  2 | On Loan: No      |
| WESTCHESTER CNTY NY LOCAL DEV  | Westchester County Local Development Corp                             | CUSIP: 95737TEV4<br>LEI: 549300KOSU8OC75V9238 | Long             | DBT              | MUN               | US        |   6000000 | PA      | $4240500.00   | 1.92%             | 2056-07-01      | Fixed         | 4.50%                 | No            |                  2 | On Loan: No      |
| FLORIDA ST TURNPIKE AUTH TURNP | State of Florida Department of Transportation Turnpike System Revenue | CUSIP: 343137LE3<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   3700000 | PA      | $2324414.00   | 1.05%             | 2046-07-01      | Fixed         | 2.12%                 | No            |                  2 | On Loan: No      |
| Housing Opportunities Comm of  | Housing Opportunities Commission of Montgomery County                 | CUSIP: 613347PM9<br>LEI: 549300HDL0LLRHMDL092 | Long             | ABS-MBS          | CORP              | US        |   1500000 | PA      | $986880.00    | 0.45%             | 2052-07-01      | Fixed         | 2.10%                 | No            |                  2 | On Loan: No      |
| ERIE CNTY NY TOBACCO ASSET SEC | Erie Tobacco Asset Securitization Corp                                | CUSIP: 295095BB8<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   4385000 | PA      | $4342377.80   | 1.97%             | 2045-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| BUCKEYE OH TOBACCO SETTLEMENT  | Buckeye Tobacco Settlement Financing Authority                        | CUSIP: 118217CZ9<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |  18590000 | PA      | $17702699.30  | 8.01%             | 2055-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| TSASC INC NY                   | TSASC Inc/NY                                                          | CUSIP: 898526FR4<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   8325000 | PA      | $7825500.00   | 3.54%             | 2048-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| NEW YORK CNTYS NY TOBACCO TRUS | New York Counties Tobacco Trust IV                                    | CUSIP: 62947YAE1<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   7915000 | PA      | $7370606.30   | 3.34%             | 2045-06-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| TULSA OK MUNI ARPT TRUST TRUST | Tulsa Airports Improvement Trust                                      | CUSIP: 899661EK4<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   5900000 | PA      | $5917641.00   | 2.68%             | 2035-12-01      | Fixed         | 5.50%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH HIGHWAY & T | Puerto Rico Highway & Transportation Authority                        | CUSIP: 745197AA1<br>LEI: 549300J6QBXVWJXB7Y41 | Long             | DBT              | MUN               | US        |   2000000 | PA      | $1925000.00   | 0.87%             | 2062-07-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| RAMPART RANGE CO MET DIST #5   | Rampart Range Metropolitan District No 5                              | CUSIP: 75157WAC6<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   1000000 | PA      | $712360.00    | 0.32%             | 2051-12-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| DENVER CITY & CNTY CO SPL FACS | City & County of Denver CO                                            | CUSIP: 249271GV3<br>LEI: MMTY7HA2MSCBUO4F7J71 | Long             | DBT              | MUN               | US        |   2000000 | PA      | $2005000.00   | 0.91%             | 2032-10-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH             | Commonwealth of Puerto Rico                                           | CUSIP: 74514L3M7<br>LEI: 549300X44HXPLJU48U63 | Long             | DBT              | MUN               | US        |  18673960 | PA      | $16594241.07  | 7.51%             | 2037-07-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| JOHNSTON CNTY NC WTR & SWR REV | County of Johnston NC Water & Sewer System Revenue                    | CUSIP: 479357AS4<br>LEI: N/A                  | Long             | DBT              | MUN               | US        |   5000000 | PA      | $3030400.00   | 1.37%             | 2051-04-01      | Fixed         | 2.25%                 | No            |                  2 | On Loan: No      |
| PUERTO RICO CMWLTH             | Commonwealth of Puerto Rico                                           | CUSIP: 74514L3N5<br>LEI: 549300X44HXPLJU48U63 | Long             | DBT              | MUN               | US        |   1796000 | PA      | $1542494.60   | 0.70%             | 2041-07-01      | Fixed         | 4.00%                 | No            |                  2 | On Loan: No      |
| CHICAGO IL BRD OF EDU          | Chicago Board of Education                                            | CUSIP: 167505PL4<br>LEI: 54930099NR6T9V7LVB43 | Long             | DBT              | MUN               | US        |   4260000 | PA      | $4207687.20   | 1.91%             | 2042-12-01      | Fixed         | 5.00%                 | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2023-03-22

**Name of Applicant:** Pioneer Municipal High Income Opportunities Fund, Inc.

**Signature:** Anthony J. Koenig, Jr

**Name of Signer:** Anthony J. Koenig, Jr

**Title:** Managing Director, Chief Operations Officer & Treasurer of the Funds