# EDGAR Filing Document

**Accession Number:** 0001870144
**File Stem:** 9999999995-25-003015
**Filing Date:** 2025-9
**Character Count:** 1504
**Document Hash:** 6b95e82997a63c8827e0f7a69bba95a7
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**9999999995-25-003015.hdr.sgml**: 20250922

**ACCESSION NUMBER**: 9999999995-25-003015

**CONFORMED SUBMISSION TYPE**: EFFECT

**PUBLIC DOCUMENT COUNT**: 1

**FILED AS OF DATE**: 20250919

**DATE AS OF CHANGE**: 20250922

**EFFECTIVENESS DATE**: 20250919

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Cardio Diagnostics Holdings, Inc.
- **CENTRAL INDEX KEY:** 0001870144
- **STANDARD INDUSTRIAL CLASSIFICATION:** IN VITRO & IN VIVO DIAGNOSTIC SUBSTANCES [2835]
- **ORGANIZATION NAME:** 03 Life Sciences
- **EIN:** 870925574
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** EFFECT
- **SEC ACT:** 1933 Act
- **SEC FILE NUMBER:** 333-283419
- **FILM NUMBER:** 251328305

**BUSINESS ADDRESS:**
- **STREET 1:** 311 W. SUPERIOR STREET
- **STREET 2:** SUITE 444
- **CITY:** CHICAGO
- **STATE:** IL
- **ZIP:** 60645
- **BUSINESS PHONE:** 855-226-9991

**MAIL ADDRESS:**
- **STREET 1:** 311 W. SUPERIOR STREET
- **STREET 2:** SUITE 444
- **CITY:** CHICAGO
- **STATE:** IL
- **ZIP:** 60645

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** Mana Capital Acquisition Corp.
- **DATE OF NAME CHANGE:** 20210629

### UNITED STATES
### SECURITIES AND EXCHANGE COMMISSION
**Washington, D.C. 20549**

## Notice of Effectiveness

**Date:** September 19, 2025

**Accession Number:** 0001079973-25-001464

**Form Type:** POS AM

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**CIK:** 0001870144

**Company Name:** Cardio Diagnostics Holdings, Inc.

**File Number:** 333-283419