# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001741773-23-000780
**Filing Date:** 2023-3
**Character Count:** 62299
**Document Hash:** 272d8c134b9e55ff4f7181a23fbfe420
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001741773-23-000780.hdr.sgml**: 20230301

**ACCESSION NUMBER**: 0001741773-23-000780

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 2

**CONFORMED PERIOD OF REPORT**: 20221231

**FILED AS OF DATE**: 20230301

**DATE AS OF CHANGE**: 20230301

**PERIOD START**: 20230930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751
- **IRS NUMBER:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 23691534

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Enterprise Fund (Series ID: S000010490)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028952 | Class T      | JAENX           |
| C000077737 | Class A      | JDMAX           |
| C000077738 | Class C      | JGRCX           |
| C000077739 | Class I      | JMGRX           |
| C000077740 | Class R      | JDMRX           |
| C000077741 | Class S      | JGRTX           |
| C000083535 | Class D      | JANEX           |
| C000114883 | Class N      | JDMNX           |

## Nport-Ex

### Janus Henderson Enterprise Fund

#### Schedule of Investments (unaudited)

#### December 31, 2022

---

| | | |
|:---|:---|:---|
| *Shares or*<br>*Principal Amounts* | *Shares or*<br>*Principal Amounts* | *Value* |
| Common Stocks– 98.0% | Common Stocks– 98.0% |  |
| Aerospace & Defense – 1.7% | Aerospace & Defense – 1.7% |  |
| L3Harris Technologies Inc | 1382480 | $287846161 |
| Airlines – 0.9% | Airlines – 0.9% |  |
| Ryanair Holdings PLC (ADR)\* | 2030937 | 151832850 |
| Auto Components – 0.5% | Auto Components – 0.5% |  |
| Visteon Corp\* | 682812 | 89332294 |
| Biotechnology – 3.1% | Biotechnology – 3.1% |  |
| Argenx SE (ADR)\* | 207746 | 78700417 |
| Ascendis Pharma A/S (ADR)\* | 999428 | 122060142 |
| BioMarin Pharmaceutical Inc\* | 1595564 | 165124918 |
| Sarepta Therapeutics Inc\* | 1073081 | 139049836 |
|  |  | 504935313 |
| Capital Markets – 4.7% | Capital Markets – 4.7% |  |
| Cboe Global Markets Inc | 908899 | 114039558 |
| Charles Schwab Corp | 2594459 | 216014656 |
| LPL Financial Holdings Inc | 1672814 | 361612202 |
| MSCI Inc | 181334 | 84351137 |
|  |  | 776017553 |
| Chemicals – 0.8% | Chemicals – 0.8% |  |
| Corteva Inc | 2178102 | 128028836 |
| Commercial Services & Supplies – 2.8% | Commercial Services & Supplies – 2.8% |  |
| Cimpress PLC\*<sup>,£</sup> | 2154740 | 59492371 |
| Rentokil Initial PLC | 4436094 | 27259709 |
| Rentokil Initial PLC (ADR) | 6444930 | 198568293 |
| Ritchie Bros Auctioneers Inc | 2948037 | 170484980 |
|  |  | 455805353 |
| Containers & Packaging – 0.9% | Containers & Packaging – 0.9% |  |
| Sealed Air Corp | 3101763 | 154715938 |
| Diversified Consumer Services – 0.4% | Diversified Consumer Services – 0.4% |  |
| Frontdoor Inc\* | 3437401 | 71497941 |
| Electric Utilities – 0.7% | Electric Utilities – 0.7% |  |
| Alliant Energy Corp | 2107613 | 116361314 |
| Electrical Equipment – 2.1% | Electrical Equipment – 2.1% |  |
| Regal Beloit Corp | 568491 | 68207550 |
| Sensata Technologies Holding PLC | 7037320 | 284166982 |
|  |  | 352374532 |
| Electronic Equipment, Instruments & Components – 8.2% | Electronic Equipment, Instruments & Components – 8.2% |  |
| Flex Ltd\* | 18018604 | 386679242 |
| National Instruments Corp | 5780684 | 213307240 |
| TE Connectivity Ltd | 3494938 | 401218882 |
| Teledyne Technologies Inc\* | 865827 | 346252876 |
|  |  | 1347458240 |
| Entertainment – 1.9% | Entertainment – 1.9% |  |
| Liberty Media Corp-Liberty Formula One\* | 5366846 | 320830054 |
| Equity Real Estate Investment Trusts (REITs) – 1.4% | Equity Real Estate Investment Trusts (REITs) – 1.4% |  |
| Lamar Advertising Co | 2400381 | 226595966 |
| Health Care Equipment & Supplies – 9.4% | Health Care Equipment & Supplies – 9.4% |  |
| Boston Scientific Corp\* | 10832825 | 501234813 |
| Cooper Cos Inc | 515501 | 170460716 |
| Dentsply Sirona Inc | 4421283 | 140773651 |
| ICU Medical Inc\*<sup>,£</sup> | 1241382 | 195492837 |
| STERIS PLC | 1002052 | 185068984 |
| Teleflex Inc | 1426156 | 356011322 |
|  |  | 1549042323 |
| Hotels, Restaurants & Leisure – 2.8% | Hotels, Restaurants & Leisure – 2.8% |  |
| Aramark | 6810731 | 281555620 |
| Entain PLC | 10762233 | 172521474 |
|  |  | 454077094 |
| Information Technology Services – 14.3% | Information Technology Services – 14.3% |  |
| Amdocs Ltd | 5484437 | 498535323 |
| Broadridge Financial Solutions Inc | 1636063 | 219445130 |
| Fidelity National Information Services Inc | 2652558 | 179976060 |
| Global Payments Inc | 1982747 | 196926432 |
| GoDaddy Inc\* | 5769561 | 431678554 |
| SS&C Technologies Holdings Inc | 8676112 | 451678391 |
| WEX Inc\*<sup>,£</sup> | 2342299 | 383317231 |
|  |  | 2361557121 |
| Insurance – 6.0% | Insurance – 6.0% |  |
| Intact Financial Corp | 3446752 | 496237577 |

---

------

---

| | | |
|:---|:---|:---|
| *Shares or*<br>*Principal Amounts* | *Shares or*<br>*Principal Amounts* | *Value* |
| Common Stocks– (continued) | Common Stocks– (continued) |  |
| Insurance– (continued) | Insurance– (continued) |  |
| Ryan Specialty Group Holdings Inc - Class A\* | 3055362 | $126828077 |
| WR Berkley Corp | 4969046 | 360603668 |
|  |  | 983669322 |
| Interactive Media & Services – 0.6% | Interactive Media & Services – 0.6% |  |
| Ziff Davis Inc\* | 1213356 | 95976460 |
| Internet & Direct Marketing Retail – 0% | Internet & Direct Marketing Retail – 0% |  |
| Wayfair Inc - Class A\*<sup>,#</sup> | 191417 | 6295705 |
| Life Sciences Tools & Services – 3.5% | Life Sciences Tools & Services – 3.5% |  |
| Avantor Inc\* | 8290749 | 174851896 |
| Illumina Inc\* | 651919 | 131818022 |
| PerkinElmer Inc | 1262833 | 177074443 |
| Waters Corp\* | 293482 | 100541064 |
|  |  | 584285425 |
| Machinery – 3.7% | Machinery – 3.7% |  |
| Ingersoll Rand Inc | 6369458 | 332804180 |
| Wabtec Corp | 2699268 | 269413939 |
|  |  | 602218119 |
| Multiline Retail – 0.4% | Multiline Retail – 0.4% |  |
| Dollar Tree Inc\* | 508758 | 71958732 |
| Oil, Gas & Consumable Fuels – 2.2% | Oil, Gas & Consumable Fuels – 2.2% |  |
| Magellan Midstream Partners LP | 7147831 | 358892594 |
| Pharmaceuticals – 1.3% | Pharmaceuticals – 1.3% |  |
| Catalent Inc\* | 3023857 | 136103804 |
| Elanco Animal Health Inc\* | 6520644 | 79682270 |
|  |  | 215786074 |
| Professional Services – 0.1% | Professional Services – 0.1% |  |
| Upwork Inc\* | 1795397 | 18743945 |
| Road & Rail – 3.1% | Road & Rail – 3.1% |  |
| JB Hunt Transport Services Inc | 2268577 | 395549086 |
| TFI International Inc | 1138418 | 114115020 |
|  |  | 509664106 |
| Semiconductor & Semiconductor Equipment – 8.8% | Semiconductor & Semiconductor Equipment – 8.8% |  |
| KLA Corp | 440646 | 166136761 |
| Lam Research Corp | 243093 | 102171988 |
| Microchip Technology Inc | 3891004 | 273343031 |
| NXP Semiconductors NV | 1815695 | 286934281 |
| ON Semiconductor Corp\* | 10126024 | 631560117 |
|  |  | 1460146178 |
| Software – 7.0% | Software – 7.0% |  |
| Atlassian Corp - Class A\* | 471028 | 60611883 |
| Ceridian HCM Holding Inc\* | 3398171 | 217992670 |
| Constellation Software Inc/Canada | 322346 | 503343589 |
| Dynatrace Inc\* | 2531693 | 96963842 |
| Nice Ltd (ADR)\* | 1185319 | 227936844 |
| Topicus.com Inc\* | 857331 | 45019693 |
|  |  | 1151868521 |
| Specialty Retail – 2.2% | Specialty Retail – 2.2% |  |
| Burlington Stores Inc\* | 749823 | 152034111 |
| CarMax Inc\* | 3515098 | 214034317 |
|  |  | 366068428 |
| Textiles, Apparel & Luxury Goods – 0.9% | Textiles, Apparel & Luxury Goods – 0.9% |  |
| Gildan Activewear Inc | 5342285 | 146378609 |
| Trading Companies & Distributors – 1.6% | Trading Companies & Distributors – 1.6% |  |
| Ferguson PLC | 2052528 | 260609480 |
| Total Common Stocks (cost $11,191,229,683) | Total Common Stocks (cost $11,191,229,683) | 16180870581 |
| Investment Companies– 1.7% | Investment Companies– 1.7% |  |
| Money Markets – 1.7% | Money Markets – 1.7% |  |
| Janus Henderson Cash Liquidity Fund LLC, 4.2633%<sup>ºº,£</sup>((cost $276,905,815) | 276888313 | 276943690 |
| Investments Purchased with Cash Collateral from Securities Lending– 0% | Investments Purchased with Cash Collateral from Securities Lending– 0% |  |
| Investment Companies – 0% | Investment Companies – 0% |  |
| Janus Henderson Cash Collateral Fund LLC, 4.1397%<sup>ºº,£</sup> | 451520 | 451520 |
| Time Deposits – 0% | Time Deposits – 0% |  |
| Royal Bank of Canada, 4.3100%, 1/3/23 | $112880 | 112880 |
| Total Investments Purchased with Cash Collateral from Securities Lending (cost $564,400) | Total Investments Purchased with Cash Collateral from Securities Lending (cost $564,400) | 564400 |
| Total Investments (total cost $11,468,699,898) – 99.7% | Total Investments (total cost $11,468,699,898) – 99.7% | 16458378671 |
| Cash, Receivables and Other Assets, net of Liabilities – 0.3% | Cash, Receivables and Other Assets, net of Liabilities – 0.3% | 46011741 |
| Net Assets – 100% | Net Assets – 100% | $16504390412 |

---

------

---

| | | |
|:---|:---|:---|
| ***Summary of Investments by Country - (Long Positions) (unaudited)*** | ***Summary of Investments by Country - (Long Positions) (unaudited)*** | ***Summary of Investments by Country - (Long Positions) (unaudited)*** |
|  |  | *% of* |
|  |  | *Investment* |
| *Country* | *Value* | *Securities* |
| United States | $14003919474 | 85.1% |
| Canada | 1475579468 | 9.0 |
| United Kingdom | 398349476 | 2.4 |
| Israel | 227936844 | 1.4 |
| Ireland | 151832850 | 0.9 |
| Denmark | 122060142 | 0.7 |
| Belgium | 78700417 | 0.5 |

---

---

| | | | |
|:---|:---|:---|:---|
| Total | $16458378671 | 100.0 | % |

---

#### Schedules of Affiliated Investments – (% of Net Assets)

---

| | | | | |
|:---|:---|:---|:---|:---|
|  | *Dividend*<br>*Income<sup>(1)</sup>* | *Realized*<br>*Gain/(Loss)<sup>(1)</sup>* | *Change in*<br>*Unrealized*<br>*Appreciation/* <br>*Depreciation<sup>(1)</sup>* | *Value*<br>*at 12/31/22* |
| Common Stocks - 3.9% | Common Stocks - 3.9% | Common Stocks - 3.9% | Common Stocks - 3.9% | Common Stocks - 3.9% |
| Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% |
| Cimpress PLC<sup>\*</sup> | $- | $- | $6744336 | $59492371 |
| Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% |
| ICU Medical Inc<sup>\*</sup> | - | - | 8540708 | 195492837 |
| Information Technology Services - 2.3% | Information Technology Services - 2.3% | Information Technology Services - 2.3% | Information Technology Services - 2.3% | Information Technology Services - 2.3% |
| WEX Inc<sup>\*</sup> | - | - | 85985796 | 383317231 |
| Total Common Stocks | $- | $- | $101270840 | $638302439 |
| Investment Companies - 1.7% | Investment Companies - 1.7% | Investment Companies - 1.7% | Investment Companies - 1.7% | Investment Companies - 1.7% |
| Money Markets - 1.7% | Money Markets - 1.7% | Money Markets - 1.7% | Money Markets - 1.7% | Money Markets - 1.7% |
| Janus Henderson Cash Liquidity Fund LLC, 4.2633%<sup>ºº</sup> | 4841681 | 62032 | 4198 | 276943690 |
| Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% |
| Investment Companies - 0.0% | Investment Companies - 0.0% | Investment Companies - 0.0% | Investment Companies - 0.0% | Investment Companies - 0.0% |
| Janus Henderson Cash Collateral Fund LLC, 4.1397%<sup>ºº</sup> | 42297<sup>∆</sup> | - | - | 451520 |
| Total Affiliated Investments - 5.6% | $4883978 | $62032 | $101275038 | $915697649 |

---

(1) For securities that were affiliated for a portion of the period ended December 31, 2022, this column reflects amounts for the entire period ended December 31, 2022 and not just the period in which the security was affiliated.

---

| | | | | |
|:---|:---|:---|:---|:---|
|  | *Value*<br> *at 9/30/22* | *Purchases* | *Sales Proceeds* | *Value*<br>*at 12/31/22* |
| Common Stocks - 3.9% | Common Stocks - 3.9% | Common Stocks - 3.9% | Common Stocks - 3.9% | Common Stocks - 3.9% |
| Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% | Commercial Services & Supplies - 0.4% |
| Cimpress PLC<sup>\*</sup> | 52748035 | - | - | 59492371 |
| Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% | Health Care Equipment & Supplies - 1.2% |
| ICU Medical Inc<sup>\*</sup> | 186952129 | - | - | 195492837 |
| Information Technology Services - 2.3% | Information Technology Services - 2.3% | Information Technology Services - 2.3% | Information Technology Services - 2.3% | Information Technology Services - 2.3% |
| WEX Inc<sup>\*</sup> | 297331435 | - | - | 383317231 |
| Investment Companies - 1.7% | Investment Companies - 1.7% | Investment Companies - 1.7% | Investment Companies - 1.7% | Investment Companies - 1.7% |
| Money Markets - 1.7% | Money Markets - 1.7% | Money Markets - 1.7% | Money Markets - 1.7% | Money Markets - 1.7% |
| Janus Henderson Cash Liquidity Fund LLC, 4.2633%<sup>ºº</sup> | 605370179 | 594930768 | (923423487) | 276943690 |
| Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% | Investments Purchased with Cash Collateral from Securities Lending - 0.0% |
| Investment Companies - 0.0% | Investment Companies - 0.0% | Investment Companies - 0.0% | Investment Companies - 0.0% | Investment Companies - 0.0% |
| Janus Henderson Cash Collateral Fund LLC, 4.1397%<sup>ºº</sup> | 22240695 | 218794007 | (240583182) | 451520 |

---

------

***Schedule of Forward Foreign Currency Exchange Contracts***<br>

---

| | | | | |
|:---|:---|:---|:---|:---|
| *Counterparty/*<br>*Foreign Currency* | *Settlement*<br>*Date* | *Foreign Currency*<br>*Amount (Sold)/*<br>*Purchased* | *USD Currency*<br>*Amount (Sold)/*<br>*Purchased* | *Market Value and*<br>*Unrealized*<br>*Appreciation/*<br>(Depreciation) |
| Barclays Capital, Inc.: |  |  |  |  |
| &nbsp;&nbsp;&nbsp;Canadian Dollar | 3/16/23 | (153640000) | $112899777 | $(661503) |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | (44365000) | 47525003 | (204171) |
|  |  |  |  | (865674) |
| Citibank, National Association: |  |  |  |  |
| &nbsp;&nbsp;&nbsp;Canadian Dollar | 3/16/23 | 5300000 | (3888208) | 29227 |
| &nbsp;&nbsp;&nbsp;Canadian Dollar | 3/16/23 | (84211000) | 61841346 | (402268) |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | (69579000) | 74491375 | (363765) |
|  |  |  |  | (736806) |
| HSBC Securities (USA), Inc.: |  |  |  |  |
| &nbsp;&nbsp;&nbsp;Canadian Dollar | 3/16/23 | (116890000) | 85907471 | (490459) |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | 7700000 | (8249519) | 34368 |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | (14480000) | 15497293 | (80718) |
|  |  |  |  | (536809) |
| JPMorgan Chase Bank, National Association: |  |  |  |  |
| &nbsp;&nbsp;&nbsp;Canadian Dollar | 3/16/23 | (186046000) | 136577595 | (936215) |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | 7200000 | (7689427) | 56545 |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | (51769000) | 55457541 | (237075) |
|  |  |  |  | (1116745) |
| State Street Bank and Trust Company: |  |  |  |  |
| &nbsp;&nbsp;&nbsp;Canadian Dollar | 3/16/23 | (106540000) | 78170399 | (577445) |
| &nbsp;&nbsp;&nbsp;Euro | 3/16/23 | (74348000) | 79637860 | (347910) |
|  |  |  |  | (925355) |
| Total |  |  |  | $(4181389) |

---

---

| | |
|:---|:---|
| **Average Ending Monthly Value of Derivative Instruments During the Period Ended December 31, 2022** | **Average Ending Monthly Value of Derivative Instruments During the Period Ended December 31, 2022** |
| Forward foreign currency exchange contracts: |  |
| Average amounts purchased - in USD | $103836070  |
| Average amounts sold - in USD | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;799723074  |

---

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#### Notes to Schedule of Investments (unaudited)

---

| | |
|:---|:---|
| ADR | American Depositary Receipt |
| LLC | Limited Liability Company |
| LP | Limited Partnership |
| PLC | Public Limited Company |

---

<br> \* Non-income producing security.

<br> ºº Rate shown is the 7-day yield as of December 31, 2022.

<br> # Loaned security; a portion of the security is on loan at December 31, 2022.

£The Fund may invest in certain securities that are considered affiliated companies. As defined by the Investment Company Act of 1940, as amended, an affiliated company is one in which the Fund owns 5% or more of the outstanding voting securities, or a company which is under common ownership or control.<br>

∆ Net of income paid to the securities lending
agent and rebates paid to the borrowing counterparties.

---

| | | | | |
|:---|:---|:---|:---|:---|
| The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. |
| **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** |
|  |  |  | ***Level 2 -*** | ***Level 3 -*** |
|  |  | ***Level 1 -*** | ***Other Significant*** | ***Significant*** |
|  |  | ***Quoted Prices*** | ***Observable Inputs*** | ***Unobservable Inputs*** |
| **Assets** | **Assets** |  |  |  |
| **Investments In Securities:** | **Investments In Securities:** |  |  |  |
| *Common Stocks* | *Common Stocks* |  |  |  |
| Commercial Services & Supplies | Commercial Services & Supplies | $428545644 | $27259709 | $- |
| Hotels, Restaurants & Leisure | Hotels, Restaurants & Leisure | 281555620 | 172521474 | - |
| All Other | All Other | 15270988134 | - | - |
| *Investment Companies* | *Investment Companies* | - | 276943690 | - |
| *Investments Purchased with Cash Collateral from Securities Lending* | *Investments Purchased with Cash Collateral from Securities Lending* | - | 564400 | - |
| Total Investments in Securities | Total Investments in Securities | $15981089398 | $477289273 | $- |
| **Other Financial Instruments<sup>(a)</sup>:** | **Other Financial Instruments<sup>(a)</sup>:** |  |  |  |
| *Forward Foreign Currency Exchange Contracts* | *Forward Foreign Currency Exchange Contracts* | - | 120140 | - |
| **Total Assets** | **Total Assets** | $15981089398 | $477409413 | $- |
| **Liabilities** | **Liabilities** |  |  |  |
| **Other Financial Instruments<sup>(a)</sup>:** | **Other Financial Instruments<sup>(a)</sup>:** |  |  |  |
| *Forward Foreign Currency Exchange Contracts* | *Forward Foreign Currency Exchange Contracts* | $- | $4301529 | $- |
| (a) | Other financial instruments may include forward foreign currency exchange contracts, futures, written options, written swaptions, and swap contracts. Forward foreign currency exchange contracts, futures contracts, and swap contracts are reported at their unrealized appreciation/(depreciation) at measurement date, which represents the change in the contract's value from trade date. Written options and written swaptions are reported at their market value at measurement date. | Other financial instruments may include forward foreign currency exchange contracts, futures, written options, written swaptions, and swap contracts. Forward foreign currency exchange contracts, futures contracts, and swap contracts are reported at their unrealized appreciation/(depreciation) at measurement date, which represents the change in the contract's value from trade date. Written options and written swaptions are reported at their market value at measurement date. | Other financial instruments may include forward foreign currency exchange contracts, futures, written options, written swaptions, and swap contracts. Forward foreign currency exchange contracts, futures contracts, and swap contracts are reported at their unrealized appreciation/(depreciation) at measurement date, which represents the change in the contract's value from trade date. Written options and written swaptions are reported at their market value at measurement date. | Other financial instruments may include forward foreign currency exchange contracts, futures, written options, written swaptions, and swap contracts. Forward foreign currency exchange contracts, futures contracts, and swap contracts are reported at their unrealized appreciation/(depreciation) at measurement date, which represents the change in the contract's value from trade date. Written options and written swaptions are reported at their market value at measurement date. |

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------

#### Investment Valuation
Fund holdings are valued in accordance with policies and procedures established by the Adviser pursuant to Rule 2a-5 under the 1940 Act and approved by and subject to the oversight of the Trustees (the "Valuation Procedures"). Equity securities traded on a domestic securities exchange are generally valued at readily available market quotations, which are (i) the official close prices or (ii) last sale prices on the primary market or exchange in which the securities trade. If such price is lacking for the trading period immediately preceding the time of determination, such securities are generally valued at their current bid price. Equity securities that are traded on a foreign exchange are generally valued at the closing prices on such markets. In the event that there is no current trading volume on a particular security in such foreign exchange, the bid price from the primary exchange is generally used to value the security. Foreign securities and currencies are converted to U.S. dollars using the current spot USD dollar exchange rate in effect at the close of the New York Stock Exchange ("NYSE"). The Fund will determine the market value of individual securities held by it by using prices provided by one or more approved professional pricing services or, as needed, by obtaining market quotations from independent broker-dealers. Most debt securities are valued in accordance with the evaluated bid price supplied by the Adviser-approved pricing service that is intended to reflect market value. The evaluated bid price supplied by the pricing service is an evaluation that may consider factors such as security prices, yields, maturities and ratings. Certain short-term securities maturing within 60 days or less may be evaluated and valued on an amortized cost basis provided that the amortized cost determined approximates market value. Securities for which market quotations or evaluated prices are not readily available or deemed unreliable are valued at fair value determined in good faith by the Adviser pursuant to the Valuation Procedures. Circumstances in which fair valuation may be utilized include, but are not limited to: (i) a significant event that may affect the securities of a single issuer, such as a merger, bankruptcy, or significant issuer-specific development; (ii) an event that may affect an entire market, such as a natural disaster or significant governmental action; (iii) a nonsignificant event such as a market closing early or not opening, or a security trading halt; and (iv) pricing of a nonvalued security and a restricted or nonpublic security. Special valuation considerations may apply with respect to "odd-lot" fixed-income transactions which, due to their small size, may receive evaluated prices by pricing services which reflect a large block trade and not what actually could be obtained for the odd-lot position. The valuation policies provide for the use of systematic fair valuation models provided by independent pricing services to value foreign equity securities in order to adjust for stale pricing, which may occur between the close of certain foreign exchanges and the close of the NYSE. The value of the securities of other mutual funds held by a Fund, if any, will be calculated using the NAV of such mutual funds.

#### Valuation Inputs Summary
FASB ASC 820, Fair Value Measurements and Disclosures ("ASC 820"), defines fair value, establishes a framework for measuring fair value, and expands disclosure requirements regarding fair value measurements. This standard emphasizes that fair value is a market-based measurement that should be determined based on the assumptions that market participants would use in pricing an asset or liability and establishes a hierarchy that prioritizes inputs to valuation techniques used to measure fair value. These inputs are summarized into three broad levels:

Level 1 – Unadjusted quoted prices in active markets the Fund has the ability to access for identical assets or liabilities.

Level 2 – Observable inputs other than unadjusted quoted prices included in Level 1 that are observable for the asset or liability either directly or indirectly. These inputs may include quoted prices for the identical instrument on an inactive market, prices for similar instruments, interest rates, prepayment speeds, credit risk, yield curves, default rates and similar data.

Assets or liabilities categorized as Level 2 in the hierarchy generally include: debt securities fair valued in accordance with the evaluated bid or ask prices supplied by a pricing service; securities traded on OTC markets and listed securities for which no sales are reported that are fair valued at the latest bid price (or yield equivalent thereof) obtained from one or more dealers transacting in a market for such securities or by a pricing service approved by the Fund's Trustees; certain short-term debt securities with maturities of 60 days or less that are fair valued at amortized cost; and equity securities of foreign issuers whose fair value is determined by using systematic fair valuation models provided by independent third parties in order to adjust for stale pricing which may occur between the close of certain foreign exchanges and the close of the NYSE. Other securities that may be categorized as Level 2 in the hierarchy include, but are not limited to, preferred stocks, bank loans, swaps, investments in unregistered investment companies, options, and forward contracts.

Level 3 – Unobservable inputs for the asset or liability to the extent that relevant observable inputs are not available, representing the Fund's own assumptions about the assumptions that a market participant would use in valuing the asset or liability, and that would be based on the best information available.

There have been no significant changes in valuation techniques used in valuing any such positions held by the Fund since the beginning of the fiscal year.

The inputs or methodology used for fair valuing securities are not necessarily an indication of the risk associated with investing in those securities. The summary of inputs used as of December 31, 2022 to fair value the Fund's investments

------

in securities and other financial instruments is included in the "Valuation Inputs Summary" in the Notes to Schedule of Investments.

------

For additional information on the Fund, please refer to the Fund's most recent semiannual or annual shareholder report.

125-25-70218 03-23<br>

------

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 DETROIT STREET

  - **City:** DENVER

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 3033333863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Enterprise Fund

- **b. EDGAR series identifier (if any):** S000010490

- **c. LEI of Series:** TJ2Y3QQ1QRI8KQ84FR29

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2023-09-30

- **b. Date as of which information is reported:** 2022-12-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $16529651791.00

**Total Liabilities:** $25270400.00

**Net Assets:** $16504381391.00

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000077737 | -4.33%               | 5.28%                | 8.62%                |
| Class ID C000077738 | -4.36%               | 5.22%                | 8.57%                |
| Class ID C000083535 | -4.30%               | 5.31%                | 8.65%                |
| Class ID C000077739 | -4.29%               | 5.31%                | 8.65%                |
| Class ID C000114883 | -4.29%               | 5.32%                | 8.67%                |
| Class ID C000077740 | -4.35%               | 5.26%                | 8.59%                |
| Class ID C000077741 | -4.33%               | 5.28%                | 8.62%                |
| Class ID C000028952 | -4.31%               | 5.30%                | 8.64%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $178829522.00            | $-946148373.00                             |
| Month 2  | $231961538.00            | $675368130.00                              |
| Month 3  | $181852514.00            | $1204639805.00                             |

**Designated Index Information**

- **Index Name:** Russell Midcap Growth TR Index

- **Index Identifier:** RUSSELL.MC.D94641

### Schedule of Portfolio Investments

| Name                                 | Title                                      | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| N/A                                  | Royal Bank of Canada                       | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |    112880 | PA      | $112880.00    | 0.00%             | 2023-01-03      | Fixed         | 4.31%                 | No            |                  2 | On Loan: No      |
| Alliant Energy Corp                  | Alliant Energy Corp                        | CUSIP: 018802108<br>LEI: 5493009ML300G373MZ12 | Long             | EC               | CORP              | US        |   2107613 | NS      | $116361314.00 | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Aramark                              | Aramark                                    | CUSIP: 03852U106<br>LEI: 549300MR6PG2DWZUIL39 | Long             | EC               | CORP              | US        |   6810731 | NS      | $281555620.00 | 1.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlassian Corp                       | Atlassian Corp - Class A                   | CUSIP: 049468101<br>LEI: 549300V7ZY5P02D2MY38 | Long             | EC               | CORP              | US        |    471028 | NS      | $60611883.00  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Avantor Inc                          | Avantor Inc                                | CUSIP: 05352A100<br>LEI: 5493003VM0XB4U8YD238 | Long             | EC               | CORP              | US        |   8290749 | NS      | $174851896.00 | 1.06%             |  |  |  | No            |                  1 | On Loan: No      |
| W R Berkley Corp                     | WR Berkley Corp                            | CUSIP: 084423102<br>LEI: SQOAGCLKBDWNVYV1OV80 | Long             | EC               | CORP              | US        |   5069134 | NS      | $367867054.00 | 2.23%             |  |  |  | No            |                  1 | On Loan: No      |
| BioMarin Pharmaceutical Inc          | BioMarin Pharmaceutical Inc                | CUSIP: 09061G101<br>LEI: NSLL8ITTRR0J5HEMR848 | Long             | EC               | CORP              | US        |   1595564 | NS      | $165124918.00 | 1.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corp               | Boston Scientific Corp                     | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |  10832825 | NS      | $501234813.00 | 3.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadridge Financial Solutions       | Broadridge Financial Solutions Inc         | CUSIP: 11133T103<br>LEI: 549300KZDJZQ2YIHRC28 | Long             | EC               | CORP              | US        |   1636063 | NS      | $219445130.00 | 1.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Burlington Stores Inc                | Burlington Stores Inc                      | CUSIP: 122017106<br>LEI: 5299003Z37DVGKKC1W09 | Long             | EC               | CORP              | US        |    793935 | NS      | $160978261.00 | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Cboe Global Markets Inc              | Cboe Global Markets Inc                    | CUSIP: 12503M108<br>LEI: 529900RLNSGA90UPEH54 | Long             | EC               | CORP              | US        |    908899 | NS      | $114039557.00 | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| CarMax Inc                           | CarMax Inc                                 | CUSIP: 143130102<br>LEI: 529900F60CID3NCZM330 | Long             | EC               | CORP              | US        |   3515098 | NS      | $214034317.00 | 1.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Catalent Inc                         | Catalent Inc                               | CUSIP: 148806102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   3023857 | NS      | $136103804.00 | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Ceridian HCM Holding Inc             | Ceridian HCM Holding Inc                   | CUSIP: 15677J108<br>LEI: 549300T64GVCHFJ8L449 | Long             | EC               | CORP              | US        |   3398171 | NS      | $217992670.00 | 1.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Cooper Cos Inc/The                   | Cooper Cos Inc                             | CUSIP: 216648402<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |    556712 | NS      | $184087957.00 | 1.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Corteva Inc                          | Corteva Inc                                | CUSIP: 22052L104<br>LEI: 549300WZN9I2QKLS0O94 | Long             | EC               | CORP              | US        |   2178102 | NS      | $128028836.00 | 0.78%             |  |  |  | No            |                  1 | On Loan: No      |
| DENTSPLY SIRONA Inc                  | DENTSPLY SIRONA Inc                        | CUSIP: 24906P109<br>LEI: 9NHEHR1SOZCYXDO8HO28 | Long             | EC               | CORP              | US        |   4421283 | NS      | $140773651.00 | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Dollar Tree Inc                      | Dollar Tree Inc                            | CUSIP: 256746108<br>LEI: 549300PMSTQITB1WHR43 | Long             | EC               | CORP              | US        |    508758 | NS      | $71958731.00  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Dynatrace Inc                        | Dynatrace Inc                              | CUSIP: 268150109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2531693 | NS      | $96963842.00  | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Elanco Animal Health Inc             | Elanco Animal Health Inc                   | CUSIP: 28414H103<br>LEI: 549300SHPNDCE059M934 | Long             | EC               | CORP              | US        |   6520644 | NS      | $79682270.00  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Fidelity National Information        | Fidelity National Information Services Inc | CUSIP: 31620M106<br>LEI: 6WQI0GK1PRFVBA061U48 | Long             | EC               | CORP              | US        |   2652558 | NS      | $179976060.00 | 1.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Frontdoor Inc                        | frontdoor Inc                              | CUSIP: 35905A109<br>LEI: 5493008RB0JZJI3QGZ06 | Long             | EC               | CORP              | US        |   3437401 | NS      | $71497941.00  | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Gildan Activewear Inc                | Gildan Activewear Inc                      | CUSIP: 375916103<br>LEI: 549300SB3IBI34M00Z45 | Long             | EC               | CORP              | CA        |   5342285 | NS      | $146378609.00 | 0.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Global Payments Inc                  | Global Payments Inc                        | CUSIP: 37940X102<br>LEI: 549300NOMHGVQBX6S778 | Long             | EC               | CORP              | US        |   1982747 | NS      | $196926432.00 | 1.19%             |  |  |  | No            |                  1 | On Loan: No      |
| GoDaddy Inc                          | GoDaddy Inc                                | CUSIP: 380237107<br>LEI: 549300J8H1TB0I2CB447 | Long             | EC               | CORP              | US        |   5769561 | NS      | $431678554.00 | 2.62%             |  |  |  | No            |                  1 | On Loan: No      |
| JB Hunt Transport Services Inc       | JB Hunt Transport Services Inc             | CUSIP: 445658107<br>LEI: 549300XCD1MPI1C5GK90 | Long             | EC               | CORP              | US        |   2289488 | NS      | $399195128.00 | 2.42%             |  |  |  | No            |                  1 | On Loan: No      |
| ICU Medical Inc                      | ICU Medical Inc                            | CUSIP: 44930G107<br>LEI: 5493003NRW7LS2QZRH60 | Long             | EC               | CORP              | US        |   1241382 | NS      | $195492837.00 | 1.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc                         | ILLUMINA INC                               | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |    651919 | NS      | $131818022.00 | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Ingersoll Rand Inc                   | Ingersoll Rand Inc                         | CUSIP: 45687V106<br>LEI: 5299004C02FMZCUOIR50 | Long             | EC               | CORP              | US        |   6369458 | NS      | $332804180.00 | 2.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Ziff Davis Inc                       | j2 Global Inc                              | CUSIP: 48123V102<br>LEI: 5493008OJYGLHD7MXA18 | Long             | EC               | CORP              | US        |   1213356 | NS      | $95976460.00  | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp                             | KLA Corp                                   | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    484726 | NS      | $182756244.00 | 1.11%             |  |  |  | No            |                  1 | On Loan: No      |
| LPL Financial Holdings Inc           | LPL Financial Holdings Inc                 | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |   1672814 | NS      | $361612202.00 | 2.19%             |  |  |  | No            |                  1 | On Loan: No      |
| L3Harris Technologies Inc            | L3Harris Technologies Inc                  | CUSIP: 502431109<br>LEI: 549300UTE50ZMDBG8A20 | Long             | EC               | CORP              | US        |   1382480 | NS      | $287846161.00 | 1.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corp                    | LAM RESEARCH CORP                          | CUSIP: 512807108<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |    243093 | NS      | $102171988.00 | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Lamar Advertising Co                 | Lamar Advertising Co                       | CUSIP: 512816109<br>LEI: 529900Y8WW4MKVPTB543 | Long             | EC               | CORP              | US        |   2400381 | NS      | $226595966.00 | 1.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp-Liberty For       | Liberty Media Corp-Liberty Formula One     | CUSIP: 531229854<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |   5366846 | NS      | $320830054.00 | 1.94%             |  |  |  | No            |                  1 | On Loan: No      |
| MSCI Inc                             | MSCI Inc                                   | CUSIP: 55354G100<br>LEI: 549300HTIN2PD78UB763 | Long             | EC               | CORP              | US        |    181334 | NS      | $84351137.00  | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Magellan Midstream Partners LP       | Magellan Midstream Partners LP             | CUSIP: 559080106<br>LEI: MZF5TI8NFVZZNUSKDL39 | Long             | EC               | CORP              | US        |   7147831 | NS      | $358892594.00 | 2.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Microchip Technology Inc             | MICROCHIP TECHNOLOGY INC                   | CUSIP: 595017104<br>LEI: 5493007PTFULNYZJ1R12 | Long             | EC               | CORP              | US        |   3891004 | NS      | $273343031.00 | 1.66%             |  |  |  | No            |                  1 | On Loan: No      |
| National Instruments Corp            | National Instruments Corp                  | CUSIP: 636518102<br>LEI: 549300TP4OOPCYWDUP86 | Long             | EC               | CORP              | US        |   5780684 | NS      | $213307240.00 | 1.29%             |  |  |  | No            |                  1 | On Loan: No      |
| ON Semiconductor Corp                | ON Semiconductor Corp                      | CUSIP: 682189105<br>LEI: ZV20P4CNJVT8V1ZGJ064 | Long             | EC               | CORP              | US        |  10126024 | NS      | $631560117.00 | 3.83%             |  |  |  | No            |                  1 | On Loan: No      |
| PerkinElmer Inc                      | PERKINELMER INC                            | CUSIP: 714046109<br>LEI: 549300IKL1SDPFI7N655 | Long             | EC               | CORP              | US        |   1262833 | NS      | $177074443.00 | 1.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Regal Rexnord Corp                   | Regal Beloit Corp                          | CUSIP: 758750103<br>LEI: QH78R09VCJGQKPBPYU33 | Long             | EC               | CORP              | US        |    568491 | NS      | $68207550.00  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Ritchie Bros Auctioneers Inc         | Ritchie Bros Auctioneers Inc               | CUSIP: 767744105<br>LEI: 5493002H8U24DJXQ4886 | Long             | EC               | CORP              | US        |   2948037 | NS      | $170484980.00 | 1.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Ryan Specialty Holdings Inc          | Ryan Specialty Group Holdings Inc CL A     | CUSIP: 78351F107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   3055362 | NS      | $126828077.00 | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| SS&C Technologies Holdings Inc       | SS&C Technologies Holdings Inc             | CUSIP: 78467J100<br>LEI: 529900POY8H7NPPNKK71 | Long             | EC               | CORP              | US        |   8676112 | NS      | $451678391.00 | 2.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Sarepta Therapeutics Inc             | Sarepta Therapeutics Inc                   | CUSIP: 803607100<br>LEI: 549300IKDPIED8J8IG21 | Long             | EC               | CORP              | US        |   1073081 | NS      | $139049836.00 | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Charles Schwab Corp/The              | Charles Schwab Corp                        | CUSIP: 808513105<br>LEI: 549300VSGCJ7E698NM85 | Long             | EC               | CORP              | US        |   2594459 | NS      | $216014656.00 | 1.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Sealed Air Corp                      | Sealed Air Corp                            | CUSIP: 81211K100<br>LEI: Q0M0CZB4YN6JZ65WXI09 | Long             | EC               | CORP              | US        |   3101763 | NS      | $154715938.00 | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| TFI International Inc                | TFI International Inc                      | CUSIP: 87241L109<br>LEI: 5493007R9W7KO7SJN678 | Long             | EC               | CORP              | CA        |   1101469 | NS      | $110411253.00 | 0.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Teledyne Technologies Inc            | Teledyne Technologies Inc                  | CUSIP: 879360105<br>LEI: 549300VSMO9KYQWDND94 | Long             | EC               | CORP              | US        |    865827 | NS      | $346252876.00 | 2.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Teleflex Inc                         | Teleflex Inc                               | CUSIP: 879369106<br>LEI: UUL85LWXB4NGSWWXIQ25 | Long             | EC               | CORP              | US        |   1426156 | NS      | $356011322.00 | 2.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Janus Capital Group Inc              | Janus Henderson Cash Liquidity Fund        | CUSIP: N/A<br>LEI: 8T2ZO87FU1KVPZIG9I59       | Long             | EC               | PF                | US        | 276888313 | NS      | $276943690.00 | 1.68%             |  |  |  | No            |                  2 | On Loan: No      |
| Janus Henderson Cash Collateral Fund | Janus Henderson Cash Collateral Fund       | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |    451520 | NS      | $451520.00    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| Upwork Inc                           | Upwork Inc                                 | CUSIP: 91688F104<br>LEI: 5493004QJ6WWBXVE2S70 | Long             | EC               | CORP              | US        |   1795397 | NS      | $18743945.00  | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Visteon Corp                         | Visteon Corporation                        | CUSIP: 92839U206<br>LEI: 549300MOVLYHRW4GGW78 | Long             | EC               | CORP              | US        |    682812 | NS      | $89332294.00  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Westinghouse Air Brake Technol       | WABTEC CORP                                | CUSIP: 929740108<br>LEI: 06BTX5UWZD0GQ5N5Y745 | Long             | EC               | CORP              | US        |   2699268 | NS      | $269413939.00 | 1.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Waters Corp                          | Waters Corp                                | CUSIP: 941848103<br>LEI: 5T547R1474YC9HOD8Q74 | Long             | EC               | CORP              | US        |    317293 | NS      | $108698236.00 | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Wayfair Inc                          | Wayfair Inc - Class A                      | CUSIP: 94419L101<br>LEI: 54930027N9ZAVYFDHK53 | Long             | EC               | CORP              | US        |    191417 | NS      | $6295705.00   | 0.04%             |  |  |  | No            |                  1 | On Loan: —       |
| WEX Inc                              | WEX Inc                                    | CUSIP: 96208T104<br>LEI: T9M5IGROL1TL1G5OV478 | Long             | EC               | CORP              | US        |   2342299 | NS      | $383317231.00 | 2.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Intact Financial Corp                | INTACT FINANCIAL CORP                      | CUSIP: 45823T106<br>LEI: 5493009LXGE7Y4U9OY43 | Long             | EC               | CORP              | CA        |   3446752 | NS      | $496237577.00 | 3.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Rentokil Initial PLC                 | Rentokil Initial PLC                       | CUSIP: G7494G105<br>LEI: 549300VN4WV7Z6T14K68 | Long             | EC               | CORP              | GB        |   4436094 | NS      | $27259709.00  | 0.17%             |  |  |  | No            |                  2 | On Loan: No      |
| Constellation Software Inc/Can       | Constellation Software Inc/Canada          | CUSIP: 21037X100<br>LEI: 549300B6PYHMCTDWQV29 | Long             | EC               | CORP              | CA        |    322346 | NS      | $503343589.00 | 3.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Entain PLC                           | Entain PLC                                 | CUSIP: G427A6103<br>LEI: 213800GNI3K45LQR8L28 | Long             | EC               | CORP              | GB        |  10762233 | NS      | $172521474.00 | 1.04%             |  |  |  | No            |                  2 | On Loan: No      |
| Topicus.com Inc                      | Topicus.com Inc                            | CUSIP: 89072T102<br>LEI: N/A                  | Long             | EC               | CORP              | CA        |    857331 | NS      | $45019693.00  | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Amdocs Ltd                           | Amdocs Ltd                                 | CUSIP: G02602103<br>LEI: 549300HQV672RJ7RQV66 | Long             | EC               | CORP              | US        |   5484437 | NS      | $498535323.00 | 3.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Cimpress PLC                         | Cimpress PLC                               | CUSIP: G2143T103<br>LEI: 549300X1IFD75XISOR81 | Long             | EC               | CORP              | US        |   2154740 | NS      | $59492371.00  | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson PLC                         | Ferguson PLC                               | CUSIP: G3421J106<br>LEI: 213800DU1LGY3R2S2X42 | Long             | EC               | CORP              | US        |   2052528 | NS      | $260609480.00 | 1.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Sensata Technologies Holding P       | Sensata Technologies Holding PLC           | CUSIP: G8060N102<br>LEI: 529900MS7KFYCY9MS037 | Long             | EC               | CORP              | US        |   7037320 | NS      | $284166982.00 | 1.72%             |  |  |  | No            |                  1 | On Loan: No      |
| STERIS PLC                           | STERIS PLC                                 | CUSIP: G8473T100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1002052 | NS      | $185068984.00 | 1.12%             |  |  |  | No            |                  1 | On Loan: No      |
| TE Connectivity Ltd                  | TE CONNECTIVITY LTD                        | CUSIP: H84989104<br>LEI: 894500ZRIX9K13RHXR17 | Long             | EC               | CORP              | US        |   3494938 | NS      | $401218882.00 | 2.43%             |  |  |  | No            |                  1 | On Loan: No      |
| NXP Semiconductors NV                | NXP Semiconductors NV                      | CUSIP: N6596X109<br>LEI: 724500M9BY5293JDF951 | Long             | EC               | CORP              | US        |   1815695 | NS      | $286934281.00 | 1.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Flex Ltd                             | Flex Ltd                                   | CUSIP: Y2573F102<br>LEI: 549300EAQH74YHD07T53 | Long             | EC               | CORP              | US        |  18018604 | NS      | $386679242.00 | 2.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                            | Argenx SE (ADR)                            | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    207746 | NS      | $78700417.00  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                  | Ascendis Pharma A/S (ADR)                  | CUSIP: 04351P101<br>LEI: 549300B66JN3W3J8GH73 | Long             |  | CORP              | US        |    999428 | NS      | $122060142.00 | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Nice Ltd                             | Nice Ltd (ADR)                             | CUSIP: 653656108<br>LEI: 254900YTY4EF5Z4W0A51 | Long             |  | CORP              | US        |   1185319 | NS      | $227936844.00 | 1.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Rentokil Initial PLC                 | Rentokil Initial PLC (ADR)                 | CUSIP: 760125104<br>LEI: 549300VN4WV7Z6T14K68 | Long             |  | CORP              | US        |   6444930 | NS      | $198568293.00 | 1.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Ryanair Holdings PLC                 | Ryanair Holdings PLC (ADR)                 | CUSIP: 783513203<br>LEI: 635400BR2ROC1FVEBQ56 | Long             |  | CORP              | US        |   2030937 | NS      | $151832850.00 | 0.92%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-204171.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell CAD / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-661503.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell CAD / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-402268.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-363765.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-347910.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-237075.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell CAD / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-936215.00   | -0.01%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell CAD / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-490459.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell CAD / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-577445.00   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-2849.00     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-9228.00     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell EUR / Buy USD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-68641.00    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell USD / Buy EUR                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $56545.00     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell USD / Buy CAD                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $29227.00     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Sell USD / Buy EUR                         | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $34368.00     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2023-02-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER