# EDGAR Filing Document

**Accession Number:** 0001730463
**File Stem:** 0001193125-23-037734
**Filing Date:** 2023-2
**Character Count:** 27237
**Document Hash:** 26f2ac9f1432d5401b3a0af82fccf2e5
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-23-037734.hdr.sgml**: 20230214

**ACCESSION NUMBER**: 0001193125-23-037734

**CONFORMED SUBMISSION TYPE**: SC 13G/A

**PUBLIC DOCUMENT COUNT**: 2

**FILED AS OF DATE**: 20230214

**DATE AS OF CHANGE**: 20230214

**GROUP MEMBERS**: CHRIS HOLLOWOOD

**GROUP MEMBERS**: MARTIN MURPHY

**GROUP MEMBERS**: SYNCONA HOLDINGS LTD

**GROUP MEMBERS**: SYNCONA INVESTMENT MANAGEMENT LTD

**GROUP MEMBERS**: SYNCONA LTD

**SUBJECT COMPANY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Autolus Therapeutics plc
- **CENTRAL INDEX KEY:** 0001730463
- **STANDARD INDUSTRIAL CLASSIFICATION:** BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
- **IRS NUMBER:** 000000000
- **STATE OF INCORPORATION:** X0
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** SC 13G/A
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 005-90550
- **FILM NUMBER:** 23624103

**BUSINESS ADDRESS:**
- **STREET 1:** THE MEDIAWORKS
- **STREET 2:** 191 WOOD LANE
- **CITY:** LONDON
- **STATE:** X0
- **ZIP:** W12 7FP
- **BUSINESS PHONE:** 44 20 3829 6230

**MAIL ADDRESS:**
- **STREET 1:** THE MEDIAWORKS
- **STREET 2:** 191 WOOD LANE
- **CITY:** LONDON
- **STATE:** X0
- **ZIP:** W12 7FP

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** Autolus Therapeutics Ltd
- **DATE OF NAME CHANGE:** 20180205
**FILED BY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Syncona Portfolio Ltd
- **CENTRAL INDEX KEY:** 0001725042
- **IRS NUMBER:** 000000000
- **STATE OF INCORPORATION:** Y7
- **FISCAL YEAR END:** 0331

**FILING VALUES:**
- **FORM TYPE:** SC 13G/A

**BUSINESS ADDRESS:**
- **STREET 1:** 3RD FLOOR, ARNOLD HOUSE
- **STREET 2:** PO BOX 273, ST JULIAN'S AVENUE
- **CITY:** ST PETER PORT
- **STATE:** Y7
- **ZIP:** GY1 3RD
- **BUSINESS PHONE:** 001442039817909

**MAIL ADDRESS:**
- **STREET 1:** ARNOLD HOUSE, ST JULIAN'S AVENUE
- **STREET 2:** ST PETER PORT
- **CITY:** GUERNSEY, CHANNEL ISLANDS
- **STATE:** X0
- **ZIP:** GY1 3RD

------

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**UNITED STATES** 

**SECURITIES AND EXCHANGE COMMISSION** 

**Washington, D.C. 20549** 

------

**SCHEDULE 13G** 

**Under the Securities Exchange Act of 1934** 

**(Amendment No. 5)\*** 

------

## Autolus Therapeutics plc
**(Name of Issuer)** 

**American Depositary Shares and Ordinary Shares, nominal value $0.000042 per share** 

**(Title of Class of Securities)** 

**05280R 100\*\*** 

**(CUSIP Number)** 

**December 31, 2022** 

**(Date of Event which Requires Filing of this Statement)** 

------

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

☐ Rule 13d-1(b)

☐ Rule 13d-1(c)

☒ Rule 13d-1(d)

\* The remainder of this cover page shall be filled out for a reporting person's initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter the disclosures provided in a prior cover page.

The information required in the remainder of this cover page shall not be deemed to be "filed" for the purpose of Section 18 of the Securities Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the *Notes*).

\*\* There is no CUSIP number assigned to the ordinary shares. CUSIP number 05280R 100 has been assigned to the American Depositary Shares of the Issuer, which are quoted on the Nasdaq Global Select Market under the symbol "AUTL." Each American Depositary Share represents the right to receive one ordinary share. 

------

---

| | |
|:---|:---|
| **CUSIP No. 05280R 100** | **SCHEDULE 13G/A** |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 1 | &nbsp;&nbsp;&nbsp;&nbsp;Names of Reporting Person:<br>&nbsp;&nbsp;&nbsp;&nbsp;Syncona Portfolio Limited |
| &nbsp;&nbsp;&nbsp; 2 | &nbsp;&nbsp;&nbsp;&nbsp;Check the appropriate box if a member of a Group (see instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;(a) ☐ (b) ☒ |
| &nbsp;&nbsp;&nbsp; 3 | &nbsp;&nbsp;&nbsp;&nbsp;SEC Use Only |
| &nbsp;&nbsp;&nbsp; 4 | &nbsp;&nbsp;&nbsp;&nbsp;Citizenship or Place of Organization<br>&nbsp;&nbsp;&nbsp;&nbsp;Guernsey |

---

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 5 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 6 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 7 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 8 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 9 | &nbsp;&nbsp;&nbsp;&nbsp;Aggregate Amount Beneficially Owned by Each Reporting Person<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp;10 | &nbsp;&nbsp;&nbsp;&nbsp;Check box if the aggregate amount in row (9) excludes certain shares (See Instructions) |
| &nbsp;&nbsp;&nbsp;11 | &nbsp;&nbsp;&nbsp;&nbsp;Percent of class represented by amount in row (9)<br>&nbsp;&nbsp;&nbsp;&nbsp;19.7% (2) |
| &nbsp;&nbsp;&nbsp;12 | &nbsp;&nbsp;&nbsp;&nbsp;Type of Reporting Person (See Instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;CO |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 American Depositary Shares ("ADSs") held
directly by Syncona Portfolio Limited. Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADSs are collectively referred to as the "Ordinary Shares".

(2) Based on 173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the
reporting person on February 10, 2023.

Page 2 of 10

------

---

| | |
|:---|:---|
| **CUSIP No. 05280R 100** | **SCHEDULE 13G** |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 1 | &nbsp;&nbsp;&nbsp;&nbsp;Names of Reporting Person:<br>&nbsp;&nbsp;&nbsp;&nbsp;Syncona Holdings Limited |
| &nbsp;&nbsp;&nbsp; 2 | &nbsp;&nbsp;&nbsp;&nbsp;Check the appropriate box if a member of a Group (see instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;(a) ☐ (b) ☒ |
| &nbsp;&nbsp;&nbsp; 3 | &nbsp;&nbsp;&nbsp;&nbsp;SEC Use Only |
| &nbsp;&nbsp;&nbsp; 4 | &nbsp;&nbsp;&nbsp;&nbsp;Citizenship or Place of Organization<br>&nbsp;&nbsp;&nbsp;&nbsp;Guernsey |

---

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 5 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 6 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 7 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 8 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 9 | &nbsp;&nbsp;&nbsp;&nbsp;Aggregate Amount Beneficially Owned by Each Reporting Person<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp;10 | &nbsp;&nbsp;&nbsp;&nbsp;Check box if the aggregate amount in row (9) excludes certain shares (See Instructions) |
| &nbsp;&nbsp;&nbsp;11 | &nbsp;&nbsp;&nbsp;&nbsp;Percent of class represented by amount in row (9)<br>&nbsp;&nbsp;&nbsp;&nbsp;19.7% (2) |
| &nbsp;&nbsp;&nbsp;12 | &nbsp;&nbsp;&nbsp;&nbsp;Type of Reporting Person (See Instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;CO |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 ADSs held directly by Syncona Portfolio Limited. Each ADS
represents one ordinary share, nominal value $0.000042 per share, of the Issuer.

(2) The percentage of outstanding Ordinary Shares of the Issuer beneficially owned by Syncona Portfolio Limited is
set forth on Line 11 above. Based on 173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the reporting person on February 10, 2023.

Page 3 of 10

------

---

| | |
|:---|:---|
| **CUSIP No. 05280R 100** | **SCHEDULE 13G** |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 1 | &nbsp;&nbsp;&nbsp;&nbsp;Names of Reporting Person:<br>&nbsp;&nbsp;&nbsp;&nbsp;Syncona Investment Management Limited |
| &nbsp;&nbsp;&nbsp; 2 | &nbsp;&nbsp;&nbsp;&nbsp;Check the appropriate box if a member of a Group (see instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;(a) ☐ (b) ☒ |
| &nbsp;&nbsp;&nbsp; 3 | &nbsp;&nbsp;&nbsp;&nbsp;SEC Use Only |
| &nbsp;&nbsp;&nbsp; 4 | &nbsp;&nbsp;&nbsp;&nbsp;Citizenship or Place of Organization<br>&nbsp;&nbsp;&nbsp;&nbsp;United Kingdom |

---

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 5 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 6 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 7 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 8 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 9 | &nbsp;&nbsp;&nbsp;&nbsp;Aggregate Amount Beneficially Owned by Each Reporting Person<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp;10 | &nbsp;&nbsp;&nbsp;&nbsp;Check box if the aggregate amount in row (9) excludes certain shares (See Instructions) |
| &nbsp;&nbsp;&nbsp;11 | &nbsp;&nbsp;&nbsp;&nbsp;Percent of class represented by amount in row (9)<br>&nbsp;&nbsp;&nbsp;&nbsp;19.7% (2) |
| &nbsp;&nbsp;&nbsp;12 | &nbsp;&nbsp;&nbsp;&nbsp;Type of Reporting Person (See Instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;CO |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 ADSs held directly by Syncona Portfolio Limited. Each ADS
represents one ordinary share, nominal value $0.000042 per share, of the Issuer.

(2) The percentage of outstanding Ordinary Shares of the Issuer beneficially owned by Syncona Portfolio Limited is
set forth on Line 11 above. Based on 173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the reporting person on February 10, 2023.

Page 4 of 10

------

---

| | |
|:---|:---|
| **CUSIP No. 05280R 100** | **SCHEDULE 13G** |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 1 | &nbsp;&nbsp;&nbsp;&nbsp;Names of Reporting Person:<br>&nbsp;&nbsp;&nbsp;&nbsp;Syncona Limited |
| &nbsp;&nbsp;&nbsp; 2 | &nbsp;&nbsp;&nbsp;&nbsp;Check the appropriate box if a member of a Group (see instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;(a) ☐ (b) ☒ |
| &nbsp;&nbsp;&nbsp; 3 | &nbsp;&nbsp;&nbsp;&nbsp;SEC Use Only |
| &nbsp;&nbsp;&nbsp; 4 | &nbsp;&nbsp;&nbsp;&nbsp;Citizenship or Place of Organization<br>&nbsp;&nbsp;&nbsp;&nbsp;Guernsey |

---

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 5 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 6 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 7 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 8 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 9 | &nbsp;&nbsp;&nbsp;&nbsp;Aggregate Amount Beneficially Owned by Each Reporting Person<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp;10 | &nbsp;&nbsp;&nbsp;&nbsp;Check box if the aggregate amount in row (9) excludes certain shares (See Instructions) |
| &nbsp;&nbsp;&nbsp;11 | &nbsp;&nbsp;&nbsp;&nbsp;Percent of class represented by amount in row (9)<br>&nbsp;&nbsp;&nbsp;&nbsp;19.7% (2) |
| &nbsp;&nbsp;&nbsp;12 | &nbsp;&nbsp;&nbsp;&nbsp;Type of Reporting Person (See Instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;CO |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 ADSs held directly by Syncona Portfolio Limited. Each ADS
represents one ordinary share, nominal value $0.000042 per share, of the Issuer.

(2) The percentage of outstanding Ordinary Shares of the Issuer beneficially owned by Syncona Portfolio Limited is
set forth on Line 11 above. Based on 173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the reporting person on February 10, 2023.

Page 5 of 10

------

---

| | |
|:---|:---|
| **CUSIP No. 05280R 100** | **SCHEDULE 13G** |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 1 | &nbsp;&nbsp;&nbsp;&nbsp;Names of Reporting Person:<br>&nbsp;&nbsp;&nbsp;&nbsp;Martin Murphy |
| &nbsp;&nbsp;&nbsp; 2 | &nbsp;&nbsp;&nbsp;&nbsp;Check the appropriate box if a member of a Group (see instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;(a) ☐ (b) ☒ |
| &nbsp;&nbsp;&nbsp; 3 | &nbsp;&nbsp;&nbsp;&nbsp;SEC Use Only |
| &nbsp;&nbsp;&nbsp; 4 | &nbsp;&nbsp;&nbsp;&nbsp;Citizenship or Place of Organization<br>&nbsp;&nbsp;&nbsp;&nbsp;United Kingdom |

---

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 5 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 6 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 7 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 8 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 9 | &nbsp;&nbsp;&nbsp;&nbsp;Aggregate Amount Beneficially Owned by Each Reporting Person<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp;10 | &nbsp;&nbsp;&nbsp;&nbsp;Check box if the aggregate amount in row (9) excludes certain shares (See Instructions) |
| &nbsp;&nbsp;&nbsp;11 | &nbsp;&nbsp;&nbsp;&nbsp;Percent of class represented by amount in row (9)<br>&nbsp;&nbsp;&nbsp;&nbsp;19.7% (2) |
| &nbsp;&nbsp;&nbsp;12 | &nbsp;&nbsp;&nbsp;&nbsp;Type of Reporting Person (See Instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;IN |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 ADSs held directly by Syncona Portfolio Limited. Each ADS
represents one ordinary share, nominal value $0.000042 per share, of the Issuer.

(2) The percentage of outstanding Ordinary Shares of the Issuer beneficially owned by Syncona Portfolio Limited is
set forth on Line 11 above. Based on 173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the reporting person on February 10, 2023.

Page 6 of 10

------

---

| | |
|:---|:---|
| **CUSIP No. 05280R 100** | **SCHEDULE 13G** |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 1 | &nbsp;&nbsp;&nbsp;&nbsp;Names of Reporting Person:<br>&nbsp;&nbsp;&nbsp;&nbsp;Chris Hollowood |
| &nbsp;&nbsp;&nbsp; 2 | &nbsp;&nbsp;&nbsp;&nbsp;Check the appropriate box if a member of a Group (see instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;(a) ☐ (b) ☒ |
| &nbsp;&nbsp;&nbsp; 3 | &nbsp;&nbsp;&nbsp;&nbsp;SEC Use Only |
| &nbsp;&nbsp;&nbsp; 4 | &nbsp;&nbsp;&nbsp;&nbsp;Citizenship or Place of Organization<br>&nbsp;&nbsp;&nbsp;&nbsp;United Kingdom |

---

---

| | | |
|:---|:---|:---|
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 5 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 6 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Voting Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 7 | &nbsp;&nbsp;&nbsp;&nbsp;Sole Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;0 |
| &nbsp;&nbsp;&nbsp; Number of<br> Shares<br> Beneficially <br> Owned by<br> Each<br> Reporting<br> Person<br> With: | 8 | &nbsp;&nbsp;&nbsp;&nbsp;Shared Dispositive Power<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |

---

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp; 9 | &nbsp;&nbsp;&nbsp;&nbsp;Aggregate Amount Beneficially Owned by Each Reporting Person<br>&nbsp;&nbsp;&nbsp;&nbsp;33,527,162 (1) |
| &nbsp;&nbsp;&nbsp;10 | &nbsp;&nbsp;&nbsp;&nbsp;Check box if the aggregate amount in row (9) excludes certain shares (See Instructions) |
| &nbsp;&nbsp;&nbsp;11 | &nbsp;&nbsp;&nbsp;&nbsp;Percent of class represented by amount in row (9)<br>&nbsp;&nbsp;&nbsp;&nbsp;19.7% (2) |
| &nbsp;&nbsp;&nbsp;12 | &nbsp;&nbsp;&nbsp;&nbsp;Type of Reporting Person (See Instructions)<br>&nbsp;&nbsp;&nbsp;&nbsp;IN |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 ADSs held directly by Syncona Portfolio Limited. Each ADS
represents one ordinary share, nominal value $0.000042 per share, of the Issuer.

(2) The percentage of outstanding Ordinary Shares of the Issuer beneficially owned by Syncona Portfolio Limited is
set forth on Line 11 above. Based on 173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the reporting person on February 10, 2023.

Page 7 of 10

------

**Item 1.**

**(a)** **Name of Issuer:** Autolus Therapeutics plc

**(b)** **Address of Issuer's principal executive offices:** The Media Works, 191 Wood Lane, London W12 7FP

**Item 2.**

**(a)** **Name of reporting persons filing:** 

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(i) Syncona Portfolio Limited;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(ii) Syncona Holdings Limited;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(iii) Syncona Investment Management Limited;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(iv) Syncona Limited;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(v) Martin Murphy; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(vi) Chris Hollowood

**(b)** **Address of principal business office or, if none, residence:** 

The address of the principal business office of Syncona Portfolio Limited, Syncona Holdings Limited and Synocna Limited is Arnold House, St Julian's Avenue, St Peter Port, Guernsey GY1 3RD, Channel Islands. The address of the principal business office of Syncona Investment Management Limited, Martin Murphy and Chris Hollowood is 2nd Floor, 8 Bloomsbury Street, London WC1B 3SR, United Kingdom.

**(c)** **Citizenship:** 

Syncona Portfolio Limited, Syncona Holdings Limited and Syncona Limited's citizenship is Guernsey. Syncona Investment Management Limited, Martin Murphy and Chris Hollowood's citizenship is United Kingdom.

**(d)** **Title and class of securities:** 

Ordinary Shares, nominal value $0.000042 per share, and American Depositary Shares ("ADS"). Each ADS represents one ordinary share, nominal value $0.000042 per share, of the Issuer.

**(e)** **CUSIP No.:** 

There is no CUSIP number assigned to the ordinary shares. CUSIP number 05280R 100 has been assigned to the ADS, which are quoted on the Nasdaq Global Select Market under the symbol "AUTL."

**Item 3.** **If this statement is filed pursuant to §§ 240.13d-1(b) or 240.13d-2(b) or (c), check whether the person filing is a:** <br>

Not applicable.

**Item 4.**

The following information with respect to the ownership of Ordinary Shares of the Issuer by the Reporting Persons filing this statement on Schedule 13G/A is provided as of December 31, 2022:

Page 8 of 10

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---

| | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|
| **Reporting Persons** | **Ordinary**<br>**Shares Held**<br>**Directly (1)** | **Sole<br>Power<br>to Vote<br>or<br>Direct<br>the<br>Vote (1)** | **Shared**<br>**Power to<br>Vote or<br>Direct the<br>Vote (1)** | **Sole**<br>**Power to<br>Dispose or<br>Direct the<br>Disposition<br>(1)** | **Shared**<br>**Power to<br>Dispose or<br>Direct the<br>Disposition<br>(1)** | **Amount<br>Beneficially<br>Owned (1)** | **Percentage**<br>**of Class (2)** |
|  Syncona Portfolio Limited | 33527162 | 0 | 33527162 | 0 | 33527162 | 33527162 | 19.7% |
|  Syncona Holdings Limited | 0 | 0 | 33527162 | 0 | 33527162 | 33527162 | 19.7% |
|  Syncona Investment Management Limited | 0 | 0 | 33527162 | 0 | 33527162 | 33527162 | 19.7% |
|  Syncona Limited | 0 | 0 | 33527162 | 0 | 33527162 | 33527162 | 19.7% |
|  Martin Murphy | 0 | 0 | 33527162 | 0 | 33527162 | 33527162 | 19.7% |
|  Chris Hollowood | 0 | 0 | 33527162 | 0 | 33527162 | 33527162 | 19.7% |

---

(1) Consists of 12,180,333 ordinary shares and 21,346,829 ADSs held directly by Syncona Portfolio Limited. Each ADS
represents one ordinary share, nominal value $0.000042 per share, of the Issuer. The ordinary shares and the ADS are collectively referred to as the "Ordinary Shares".

(2) The percentage of outstanding Ordinary Shares of the Issuer beneficially owned was calculated based on
173,084,872 Ordinary Shares outstanding as of December 31, 2022 as provided by the Issuer to the reporting person on February 10, 2023.

The shares are owned directly by Syncona Portfolio Limited ("Syncona Portfolio") and indirectly by Syncona Holdings Limited ("Syncona Holdings"), Syncona Limited ("Syncona Limited"), Martin Murphy and Chris Hollowood. Syncona Portfolio is a wholly owned subsidiary of Syncona Holdings, and Syncona Holdings is a wholly controlled subsidiary of Syncona Limited, a publicly-listed company. Investment and voting decisions with respect to these shares are made by Syncona Portfolio, acting upon the recommendation of Martin Murphy and Chris Hollowood, who comprise the investment committee of Syncona Investment Management Limited, also a subsidiary of Syncona Holdings. Each of these entities disclaims beneficial ownership except to the extent of its pecuniary interest therein, if any.

**Item 5.** **Ownership of Five Percent or Less of a Class.** <br>

If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than five percent of the class of securities, check the following [ ].

**Item 6.** **Ownership of more than Five Percent on Behalf of Another Person.** <br>

Not applicable.

**Item 7.** **Identification and classification of the subsidiary which acquired the security being reported on by the parent holding company or control person.** <br>

Not applicable.

**Item 8.** **Identification and classification of members of the group.** <br>

Not applicable.

**Item 9.** **Notice of Dissolution of Group.** <br>

Not applicable.

**Item 10.** **Certifications.** <br>

Not applicable.

Page 9 of 10

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**SIGNATURE** 

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

Dated: February 14, 2023

---

| | |
|:---|:---|
| **SYNCONA PORTFOLIO LIMITED** | **SYNCONA PORTFOLIO LIMITED** |
| By: | /s/ Rob Hutchinson |
| Name: | Rob Hutchinson |
| Title: | Director |

---

---

| | |
|:---|:---|
| **SYNCONA HOLDINGS LIMITED** | **SYNCONA HOLDINGS LIMITED** |
| By: | /s/ Rob Hutchinson |
| Name: | Rob Hutchinson |
| Title: | Director |

---

---

| | |
|:---|:---|
| **SYNCONA INVESTMENT MANAGEMENT LIMITED** | **SYNCONA INVESTMENT MANAGEMENT LIMITED** |
| By: | /s/ Chris Hollowood |
| Name: | Chris Hollowood |
| Title: | CEO |

---

---

| | |
|:---|:---|
| **SYNCONA LIMITED** | **SYNCONA LIMITED** |
| By: | /s/ Rob Hutchinson |
| Name: | Rob Hutchinson |
| Title: | Director |

---

---

| |
|:---|
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;/s/ Martin Murphy<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Martin Murphy |
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;/s/ Chris Hollowood<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Chris Hollowood |

---

---

| | |
|:---|:---|
| Exhibit(s): |  |
| Exhibit 99.1: | Joint Filing Statement |

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Page 10 of 10

## Exhibit 99.1

**Exhibit 99.1** 

**AGREEMENT** 

In accordance with Rule 13d-1(k) under the Securities Exchange Act of 1934, as amended, the persons named below agree to the joint filing on behalf of each of them of a statement on Schedule 13G (including amendments thereto) with respect to the Ordinary Shares (represented by ADS, each of which represents one Ordinary Share) of Autolus Therapeutics plc and further agree that this Joint Filing Agreement be included as an Exhibit to such joint filings. It is understood and agreed that the joint filing of the Schedule 13G shall not be construed as an admission that the persons named herein constitute a group for purposes of Regulation 13D-G of the Securities Exchange Act of 1934, as amended.

Dated: February 14, 2023

---

| | |
|:---|:---|
| **SYNCONA PORTFOLIO LIMITED** | **SYNCONA PORTFOLIO LIMITED** |
| By: | /s/ Rob Hutchinson |
| Name: | Rob Hutchinson |
| Title: | Director |
| **SYNCONA HOLDINGS LIMITED** | **SYNCONA HOLDINGS LIMITED** |
| By: | /s/ Rob Hutchinson |
| Name: | Rob Hutchinson |
| Title: | Director |
| **SYNCONA INVESTMENT MANAGEMENT LIMITED** | **SYNCONA INVESTMENT MANAGEMENT LIMITED** |
| By: | /s/ Chris Hollowood |
| Name: | Chris Hollowood |
| Title: | CEO |
| **SYNCONA LIMITED** | **SYNCONA LIMITED** |
| By: | /s/ Rob Hutchinson |
| Name: | Rob Hutchinson |
| Title: | Director |

---

---

| |
|:---|
| /s/ Martin Murphy<br> Martin Murphy |
| /s/ Chris Hollowood<br> Chris Hollowood |

---