# EDGAR Filing Document

**Accession Number:** 0001806310
**File Stem:** 0001415889-25-015487
**Filing Date:** 2025-6
**Character Count:** 19801
**Document Hash:** ee0d394186be1bc97cf2f7cde941312b
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001415889-25-015487.hdr.sgml**: 20250603

**ACCESSION NUMBER**: 0001415889-25-015487

**CONFORMED SUBMISSION TYPE**: SCHEDULE 13D/A

**PUBLIC DOCUMENT COUNT**: 2

**FILED AS OF DATE**: 20250603

**DATE AS OF CHANGE**: 20250603

**SUBJECT COMPANY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Taysha Gene Therapies, Inc.
- **CENTRAL INDEX KEY:** 0001806310
- **STANDARD INDUSTRIAL CLASSIFICATION:** BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
- **ORGANIZATION NAME:** 03 Life Sciences
- **EIN:** 843199512
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** SCHEDULE 13D/A
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 005-91724
- **FILM NUMBER:** 251021799

**BUSINESS ADDRESS:**
- **STREET 1:** 3000 PEGASUS PARK DRIVE
- **STREET 2:** SUITE 1430
- **CITY:** DALLAS
- **STATE:** TX
- **ZIP:** 75247
- **BUSINESS PHONE:** (214) 612-0000

**MAIL ADDRESS:**
- **STREET 1:** 3000 PEGASUS PARK DRIVE
- **STREET 2:** SUITE 1430
- **CITY:** DALLAS
- **STATE:** TX
- **ZIP:** 75247
**FILED BY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Manning Paul B
- **CENTRAL INDEX KEY:** 0001494695

**ORGANIZATION NAME:**

**FILING VALUES:**
- **FORM TYPE:** SCHEDULE 13D/A

**MAIL ADDRESS:**
- **STREET 1:** 200 GARRETT STREET SUITE S
- **CITY:** CHARLOTTESVILLE
- **STATE:** VA
- **ZIP:** 22902

## Exhibit 99.1

**JOINT FILING AGREEMENT**

Pursuant to Rule 13d-1(k)(1) promulgated pursuant to the Securities Exchange Act of 1934, as amended, the undersigned agree that the attached Schedule 13D is being filed on behalf of each of the undersigned.

Dated: June 3, 2025

By: <u>/s/ Paul B. Manning</u>

Name: Paul B. Manning

THE PAUL B. MANNING REVOCABLE TRUST DATED MAY 10, 2000

By: <u>/s/ Paul B. Manning</u>

Name: Paul B. Manning

Title: Trustee

THE PBM 2023 GRANTOR RETAINED ANNUITY TRUST

By: <u>/s/ Paul B. Manning</u>

Name: Paul B. Manning

Title: Trustee

THE PBM 2025 GRANTOR RETAINED ANNUITY TRUST

By: <u>/s/ Paul B. Manning</u>

Name: Paul B. Manning

Title: Trustee

BKB GROWTH INVESTMENTS, LLC

By: Tiger Lily Capital, LLC, its Manager

By: <u>/s/ Paul B. Manning</u>

Name: Paul B. Manning

Title: Manager

By: <u>/s/ Bradford Manning</u>

Name: Bradford Manning

Title: Manager

BKB G2 INVESTMENTS, LLC

By: Tiger Lily Capital, LLC, its Manager

By: <u>/s/ Paul B. Manning</u>

Name: Paul B. Manning

Title: Manager

By: <u>/s/ Bradford Manning</u>

Name: Bradford Manning

Title: Manager

### UNITED STATES SECURITIES AND EXCHANGE COMMISSION
**Washington, D.C. 20549**

## SCHEDULE 13D

### Under the Securities Exchange Act of 1934

**(Amendment No. 3)**

**TAYSHA GENE THERAPIES, INC.**

*(Name of Issuer)*

**Common Stock, $0.00001 par value per share**

*(Title of Class of Securities)*

**877619106**

*(CUSIP Number)*

**Paul B. Manning**<br>c/o PBM Capital Group, LLC<br>200 Garrett Street, Suite S<br>Charlottesville VA 22902<br>(434) 980-8100

*(Name, Address and Telephone Number of Person Authorized to Receive Notices and Communications)*

**05/30/2025**

*(Date of Event Which Requires Filing of this Statement)*

| **CUSIP No.** | **877619106** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Paul B Manning** | Name of reporting person<br>**Paul B Manning** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**PF** | Source of funds (See Instructions)<br>**PF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**X1** | Citizenship or place of organization<br>**X1** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**22737407.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2863704.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**22737407.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2863704.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**25601111.00** | Aggregate amount beneficially owned by each reporting person<br>**25601111.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**9.8%** | Percent of class represented by amount in Row (11)<br>**9.8%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**IN** | Type of Reporting Person (See Instructions)<br>**IN** | |

---

| **CUSIP No.** | **877619106** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**The Paul B. Manning Revocable Trust dated May 10, 2000** | Name of reporting person<br>**The Paul B. Manning Revocable Trust dated May 10, 2000** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**VIRGINIA** | Citizenship or place of organization<br>**VIRGINIA** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**14723948.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**14723948.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**0.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**14723948.00** | Aggregate amount beneficially owned by each reporting person<br>**14723948.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**5.6%** | Percent of class represented by amount in Row (11)<br>**5.6%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **877619106** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**The PBM 2023 Grantor Retained Annuity Trust** | Name of reporting person<br>**The PBM 2023 Grantor Retained Annuity Trust** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**VIRGINIA** | Citizenship or place of organization<br>**VIRGINIA** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**4013458.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**4013458.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**0.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**4013458.00** | Aggregate amount beneficially owned by each reporting person<br>**4013458.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**1.5%** | Percent of class represented by amount in Row (11)<br>**1.5%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **877619106** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**The PBM 2025 Grantor Retained Annuity Trust** | Name of reporting person<br>**The PBM 2025 Grantor Retained Annuity Trust** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**VIRGINIA** | Citizenship or place of organization<br>**VIRGINIA** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**4000000.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**4000000.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**0.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**4000000.00** | Aggregate amount beneficially owned by each reporting person<br>**4000000.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**1.5%** | Percent of class represented by amount in Row (11)<br>**1.5%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **877619106** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**BKB Growth Investments, LLC** | Name of reporting person<br>**BKB Growth Investments, LLC** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**DELAWARE** | Citizenship or place of organization<br>**DELAWARE** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2841704.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2841704.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2841704.00** | Aggregate amount beneficially owned by each reporting person<br>**2841704.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**1.1%** | Percent of class represented by amount in Row (11)<br>**1.1%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **877619106** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**BKB G2 Investments, LLC** | Name of reporting person<br>**BKB G2 Investments, LLC** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**DELAWARE** | Citizenship or place of organization<br>**DELAWARE** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**22000.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**22000.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**22000.00** | Aggregate amount beneficially owned by each reporting person<br>**22000.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**0.0%** | Percent of class represented by amount in Row (11)<br>**0.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

**Item 1. Security and Issuer**

**(a) Title of Class of Securities:**
Common Stock, $0.00001 par value per share

**(b) Name of Issuer:**
TAYSHA GENE THERAPIES, INC.

**(c) Address of Issuer's Principal Executive Offices:**
3000 PEGASUS PARK DRIVE, SUITE 1430, DALLAS, TX, 75247

**Item 5. Interest in Securities of the Issuer**

**(a)**
As of the date hereof, the Revocable Trust is the record owner of 14,723,948.12 shares of Common Stock.

As of the date hereof, the 2023 Annuity Trust is the record owner of 4,013,458.88 shares of Common Stock.

As of the date hereof, the 2025 Annuity Trust is the record owner of 4,000,000 shares of Common Stock.

As of the date hereof, BKB is the record owner of 2,841,704 shares of Common Stock.

As of the date hereof, BKB G2 is the record owner of 22,000 shares of Common Stock.

The Revocable Trust, 2023 Annuity Trust, 2025 Annuity Trust, BKB and BKB G2 may be deemed to beneficially own 5.6%, 1.5%, 1.5%. 1.1% and 0.0%, respectively, of the Issuer's outstanding Common Stock.

Collectively, the Reporting Persons beneficially own an aggregate of 25,601,111 shares of Common Stock, which represents 9.8% of the Issuer's outstanding Common Stock.

Each percentage is calculated based upon the sum of (i) 214,661,715 shares of Common Stock outstanding as of May 15, 2025 as reported in the Issuer's Form 10-Q for the Quarterly Period Ended March 31, 2025 filed with the SEC on May 15, 2025 and (ii) 46,868,687 shares of Common Stock issued in the underwritten public offering of equity securities by the Issuer that closed on May 30, 2025

**(b)**
The Revocable Trust is the record owner of 14,723,948.12 shares of Common Stock. Mr. Manning is the Trustee of the Revocable Trust and has sole voting and dispositive power over the shares.

The 2023 Annuity Trust is the record owner of 4,013,458.88 shares of Common Stock. Mr. Manning is the Trustee of the 2023 Annuity Trust and has sole voting and dispositive power over the shares.

The 2025 Annuity Trust is the record owner of 4,000,000 shares of Common Stock. Mr. Manning is the Trustee of the 2025 Annuity Trust and has sole voting and dispositive power over the shares.

BKB is the record owner of 2,841,704 shares of Common Stock. Mr. Manning is a co-manager of the manager of BKB and has shared voting and dispositive power over the shares.

BKB G2 is the record owner of 22,000 shares of Common Stock. Mr. Manning is a co-manager of the manager of BKB G2 and has shared voting and dispositive power over the shares.

**(c)**
Except as described herein, none of the Reporting Persons have purchased or sold any shares of Common Stock during the past 60 days.

### SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Paul B Manning

**Signature:** Paul B. Manning

**Name/Title:** Paul B. Manning

**Date:** 06/03/2025

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** The Paul B. Manning Revocable Trust dated May 10, 2000

**Signature:** Paul B. Manning

**Name/Title:** Paul B. Manning, Trustee

**Date:** 06/03/2025

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** The PBM 2023 Grantor Retained Annuity Trust

**Signature:** Paul B. Manning

**Name/Title:** Paul B. Manning, Trustee

**Date:** 06/03/2025

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** The PBM 2025 Grantor Retained Annuity Trust

**Signature:** Paul B. Manning

**Name/Title:** Paul B. Manning, Trustee

**Date:** 06/03/2025

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** BKB Growth Investments, LLC

**Signature:** /s/ Paul B. Manning and /s/ Bradford Manning

**Name/Title:** By: Tiger Lily Capital, LLC, its Manager, By: Paul B. Manning, Manager and Bradford Manning, Manager

**Date:** 06/03/2025

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** BKB G2 Investments, LLC

**Signature:** /s/ Paul B. Manning and /s/ Bradford Manning

**Name/Title:** By: Tiger Lily Capital, LLC, its Manager, By: Paul B. Manning, Manager and Bradford Manning, Manager

**Date:** 06/03/2025