# EDGAR Filing Document

**Accession Number:** 0001859309
**File Stem:** 0001493152-26-017866
**Filing Date:** 2026-4
**Character Count:** 6958
**Document Hash:** 2f68348b700069d0d51c830088ac8de4
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001493152-26-017866.hdr.sgml**: 20260417

**ACCESSION NUMBER**: 0001493152-26-017866

**CONFORMED SUBMISSION TYPE**: SCHEDULE 13D/A

**PUBLIC DOCUMENT COUNT**: 1

**FILED AS OF DATE**: 20260417

**SUBJECT COMPANY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** AINOS INC
- **CENTRAL INDEX KEY:** 0001859309

**ORGANIZATION NAME:**
- **EIN:** 000000000
- **STATE OF INCORPORATION:** CA
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** SCHEDULE 13D/A
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 005-93206
- **FILM NUMBER:** 26871850

**BUSINESS ADDRESS:**
- **STREET 1:** 8880 RIO SAN DIEGO DRIVE
- **STREET 2:** SUITE 800
- **CITY:** SAN DIEGO
- **STATE:** CA
- **ZIP:** 92108
- **BUSINESS PHONE:** 213005220

**MAIL ADDRESS:**
- **STREET 1:** 8880 RIO SAN DIEGO DRIVE
- **STREET 2:** SUITE 800
- **CITY:** SAN DIEGO
- **STATE:** CA
- **ZIP:** 92108
**FILED BY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** tsai chun-hsien
- **CENTRAL INDEX KEY:** 0001859363

**ORGANIZATION NAME:**

**FILING VALUES:**
- **FORM TYPE:** SCHEDULE 13D/A

**MAIL ADDRESS:**
- **STREET 1:** 8880 RIO SAN DIEGO DRIVE
- **STREET 2:** SUITE 800
- **CITY:** SAN DIEGO
- **STATE:** CA
- **ZIP:** 92108

### UNITED STATES SECURITIES AND EXCHANGE COMMISSION
**Washington, D.C. 20549**

## SCHEDULE 13D

### Under the Securities Exchange Act of 1934

**(Amendment No. 2)**

**AINOS INC**

*(Name of Issuer)*

**Common Stock, par value $0.01 per share**

*(Title of Class of Securities)*

**—**

*(CUSIP Number)*

**Chun-Hsien Tsai**<br>14F., No. 61, Sec. 4,<br>New Taipei Boulevard, Xinzhuang District<br>New Taipei City F5 242<br>886-37-581999

*(Name, Address and Telephone Number of Person Authorized to Receive Notices and Communications)*

**04/15/2026**

*(Date of Event Which Requires Filing of this Statement)*

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Chun-Hsien Tsai** | Name of reporting person<br>**Chun-Hsien Tsai** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[x] (a)<br>[ ] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[x] (a)<br>[ ] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**OO** | Source of funds (See Instructions)<br>**OO** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**F5** | Citizenship or place of organization<br>**F5** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**710372.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**710372.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**0.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**710372.00** | Aggregate amount beneficially owned by each reporting person<br>**710372.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**8.33%** | Percent of class represented by amount in Row (11)<br>**8.33%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**IN** | Type of Reporting Person (See Instructions)<br>**IN** | |

---

**Comment for Reporting Person:** The numbers in Rows 8, 9, and 11 represents beneficial ownership of 710,372 shares of common stock, $0.01 par value (the "Common Stock"), of Ainos, Inc., a Texas corporation (the "Issuer"), which are subject to a voting agreement between the Reporting Person and Ainos Inc., a Cayman Islands corporation ("Ainos KY"). For further details, please see Item 3 and Item 6.

The percentage in Row 13 is based on the sum of (i) 7,266,011 shares of Common Stock outstanding as of March 30, 2026 as set forth in the Annual Report on Form 10-K of the Issuer filed with the SEC on March 30, 2026, (ii) 19,531 shares issued for service fee on April 1, 2026; and (iii) 1,239,000 shares of Common Stock granted and vested on April 15, 2026 under the Ainos, Inc. 2023 Stock Incentive Plan.

**Item 1. Security and Issuer**

**(a) Title of Class of Securities:**
Common Stock, par value $0.01 per share

**(b) Name of Issuer:**
AINOS INC

**(c) Address of Issuer's Principal Executive Offices:**
8880 RIO SAN DIEGO DRIVE, SUITE 800, SAN DIEGO, CA, 92108

This Amendment No. 2 ("Amendment No. 2") amends the Schedule 13D first filed with the Securities and Exchange Commission on April 11, 2025 (the "Schedule 13D"), as amended by Amendment No. 1 dated November 28, 2025, and is filed by Chun-Hsien Tsai, with respect to the common stock, par value $0.001 per share (the "Common Stock"), of Ainos, Inc. (the "Issuer"). Unless otherwise indicated, all capitalized terms used and not defined herein have the respective meanings assigned to them in the Schedule 13D.

**Item 4. Purpose of Transaction**

The Reporting Person acquired the 300,000 RSUs under the Ainos, Inc. 2023 Stock Incentive Plan.

Although the Reporting Person is the Chief Executive Officer, President, and Chairman of Board, the Reporting Person does not have any present plans or proposals that relate to or would result in any of the actions described in subparagraphs (a) through (j) of Item 4 of Schedule 13D, although, subject to the agreements described herein, the Reporting Person, at any time, and from time to time, may review, reconsider and change their position and/or change their purpose and/or develop such plans and may seek to influence management of the Issuer or the Board of Directors with respect to the business and affairs of the Issuer and may from time to time consider pursuing or proposing such matters with advisors, the Issuer, or other persons.

**Item 5. Interest in Securities of the Issuer**

**(a)**
The information requested is incorporated herein by reference to the cover pages to this Statement.

**(b)**
The information requested is incorporated herein by reference to the cover pages to this Statement.

**(c)**
The reporting person has not effected any transactions in Common Stock during the past 60 days, except as described in this Statement.

### SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Chun-Hsien Tsai

**Signature:** /s/ Chun-Hsien Tsai

**Name/Title:** Chun-Hsien Tsai

**Date:** 04/17/2026