# EDGAR Filing Document

**Accession Number:** 0001783328
**File Stem:** 0001193125-25-198768
**Filing Date:** 2025-9
**Character Count:** 37985
**Document Hash:** 7447b9ff2b1e784ae1964f88b0116a41
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-198768.hdr.sgml**: 20250909

**ACCESSION NUMBER**: 0001193125-25-198768

**CONFORMED SUBMISSION TYPE**: 8-K

**PUBLIC DOCUMENT COUNT**: 53

**CONFORMED PERIOD OF REPORT**: 20250909

**ITEM INFORMATION**: Regulation FD Disclosure

**ITEM INFORMATION**: Financial Statements and Exhibits

**FILED AS OF DATE**: 20250909

**DATE AS OF CHANGE**: 20250909

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** TScan Therapeutics, Inc.
- **CENTRAL INDEX KEY:** 0001783328
- **STANDARD INDUSTRIAL CLASSIFICATION:** BIOLOGICAL PRODUCTS (NO DIAGNOSTIC SUBSTANCES) [2836]
- **ORGANIZATION NAME:** 03 Life Sciences
- **EIN:** 825282075
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** 8-K
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 001-40603
- **FILM NUMBER:** 251301631

**BUSINESS ADDRESS:**
- **STREET 1:** 830 WINTER STREET
- **CITY:** WALTHAM
- **STATE:** MA
- **ZIP:** 02451
- **BUSINESS PHONE:** 857-399-9500

**MAIL ADDRESS:**
- **STREET 1:** 830 WINTER STREET
- **CITY:** WALTHAM
- **STATE:** MA
- **ZIP:** 02451

?xml version='1.0' encoding='ASCII'? 8-K

### UNITED STATES

### SECURITIES AND EXCHANGE COMMISSION

#### Washington, D.C. 20549

### FORM 8-K

#### CURRENT REPORT

#### Pursuant to Section 13 or 15(d)

#### of the Securities Exchange Act of 1934

#### Date of Report (Date of earliest event reported) September 9, 2025

## TSCAN THERAPEUTICS, INC.

#### (Exact name of registrant as specified in its charter)

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| | | |
|:---|:---|:---|
| **Delaware** | **001-40603** | **82-5282075** |
| **(State or other jurisdiction**<br> **of incorporation)** | **(Commission**<br> **File Number)** | **(I.R.S. Employer**<br> **Identification No.)** |

---

---

| | |
|:---|:---|
| **830 Winter Street,**<br> **Waltham, Massachusetts** | **02451** |
| **(Address of principal executive offices)** | **(Zip Code)** |

---

#### Registrant's telephone number, including area code (857) 399-9500

#### Not Applicable

#### (Former name or former address, if changed since last report)
Check the appropriate box below if the Form 8-K filing is intended to simultaneously satisfy the filing obligation of the registrant under any of the following provisions:

☐ Written communications pursuant to Rule 425 under the Securities Act (17 CFR 230.425)

☐ Soliciting material pursuant to Rule 14a-12 under the Exchange Act (17 CFR 240.14a-12)

☐ Pre-commencement communications pursuant to Rule 14d-2(b) under the Exchange Act (17 CFR 240.14d-2(b))

☐ Pre-commencement communications pursuant to Rule 13e-4(c) under the Exchange Act (17 CFR 240.13e-4(c))

Securities registered pursuant to Section 12(b) of the Act:

---

| | | |
|:---|:---|:---|
| **Title of each class** | **Trade**<br> **Symbol(s)** | **Name of each exchange**<br> **on which registered** |
| Voting Common Stock, $0.0001 par value per share | TCRX | The Nasdaq Global Market LLC |

---

Indicate by check mark whether the registrant is an emerging growth company as defined in Rule 405 of the Securities Act of 1933 (§ 230.405 of this chapter) or Rule 12b-2 of the Securities Exchange Act of 1934 (§ 240.12b-2 of this chapter).

Emerging growth company ☒

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. ☐

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| | |
|:---|:---|
| **Item 7.01** | **Regulation FD Disclosure.**  |

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On September 9, 2025, TScan Therapeutics, Inc. (the "Company") made available an updated corporate presentation (the "Presentation") that it intends to use in potential meetings with investors, analysts, and other stakeholders. The Presentation reflects revisions to the Company's anticipated development and operational milestones, including adjusted timelines for its solid tumor program, updates to the Company's pipeline for both the hematologic malignancies and solid tumor programs, and updates on its target discovery initiatives in autoimmunity. The stockholders and investors are encouraged to review the Presentation for additional details regarding the Company's programs and updated expectations.

A copy of the Presentation is attached as Exhibit 99.1 to this Current Report on Form 8-K. The Presentation will also be available in the investor relations section of the Company's website at <u>https://ir.tscan.com</u>. Information contained on the Company's website is not incorporated by reference into this Current Report on Form 8-K, and you should not consider any information on, or that can be accessed from, the Company's website as part of this Current Report on Form 8-K.

The information under this Item 7.01, including Exhibit 99.1 hereto, is being furnished and shall not be deemed "filed" for purposes of Section 18 of the Securities Exchange Act of 1934, as amended (the "Exchange Act"), or otherwise subject to the liabilities of that section, nor shall it be deemed incorporated by reference in any filing under the Securities Act of 1933, as amended, or the Exchange Act, except as expressly set forth by specific reference in such a filing*.* The Company undertakes no obligation to update, supplement or amend the material attached hereto as Exhibit 99.1.

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| | |
|:---|:---|
| **Item 9.01** | **Financial Statements and Exhibits.**  |

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(d) Exhibits:

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| | |
|:---|:---|
| **Exhibit**<br> **&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;No.** | **Description** |
| 99.1 | [Presentation, dated September 9, 2025](d125738dex991.htm) |
| 104 | Cover Page Interactive Data File (embedded within the Inline XBRL document) |

---

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#### SIGNATURE
Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned hereunto duly authorized.

---

| | | |
|:---|:---|:---|
|  | TScan Therapeutics, Inc. | TScan Therapeutics, Inc. |
| Date: September 9, 2025 | By: | /s/ Gavin MacBeath |
|  |  | Gavin MacBeath |
|  |  | Chief Executive Officer |

---

## Exhibit 99.1

![](g125738ex99_1p1g1.jpg)

Exhibit 99.1 Company Presentation September 2025

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![](g125738ex99_1p2g1.jpg)

Disclaimers and forward-looking statements This presentation and the accompanying discussion contain forward-looking TScan's plans relating to developing and commercializing its TCR-T therapy statements within the meaning of the Private Securities Litigation Reform Act of candidates, if approved, including sales strategy; estimates of the size of the 1995, including, but not limited to, express or implied statements regarding the addressable market for TScan's TCR-T therapy candidates; TScan's manufacturing Company's plans, progress, and timing relating to the Company's solid tumor capabilities and the scalable nature of its manufacturing process; TScan's programs and the presentation of data, the Company's current and future research estimates regarding expenses, future milestone payments and revenue, capital and development plans or expectations, the structure, timing and success of the requirements and needs for additional financing; TScan's expectations regarding Company's planned preclinical development, submission of INDs, and clinical trials, competition; TScan's anticipated growth strategies; TScan's ability to attract or the potential benefits of any of the Company's proprietary platforms, multiplexing, retain key personnel; TScan's ability to establish and maintain development or current or future product candidates in treating patients, the Company's ability to partnerships and collaborations; TScan's expectations regarding federal, state and fund its operating expenses and capital expenditure requirements with its existing foreign regulatory requirements; TScan's ability to obtain and maintain cash and cash equivalents, and the Company's goals and strategy. TScan intends intellectual property protection for its proprietary platform technology and our such forward-looking statements to be covered by the safe harbor provisions for product candidates; the sufficiency of TScan's existing capital resources to fund forward-looking statements contained in Section 21E of the Securities Exchange Act its future operating expenses and capital expenditure requirements; and other of 1934 and the Private Securities Litigation Reform Act of 1995. In some cases, you factors that are described in the "Risk Factors" and "Management's Discussion can identify forward-looking statements by terms such as, but not limited to, "may," and Analysis of Financial Condition and Results of Operations" sections of TScan's "might," "will," "objective," "intend," "should," "could," "can," "would," "expect," most recent Annual Report on Form 10-K and any other filings that TScan has "believe," "anticipate," "project," "target," "design," "estimate," "predict," made or may make with the SEC in the future. "potential," "plan," "on track," or similar expressions or the negative of those terms. Such forward-looking statements are based upon current expectations that involve Any forward-looking statements contained in this presentation represent TScan's risks, changes in circumstances, assumptions, and uncertainties. The express or views only as of the date hereof and should not be relied upon as representing its implied forward-looking statements included in this presentation are only views as of any subsequent date. Except as required by law, TScan explicitly predictions and are subject to a number of risks, uncertainties and assumptions, disclaims any obligation to update any forward-looking statements. including, without limitation: the beneficial characteristics, safety, efficacy, therapeutic effects and potential advantages of TScan's TCR-T therapy candidates; TScan's expectations regarding its preclinical studies being predictive of clinical trial results; the timing of the initiation, progress and expected results of TScan's preclinical studies, clinical trials and its research and development programs; 2

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![](g125738ex99_1p3g1.jpg)

TScan is a fully integrated, next-generation TCR-T therapy company Clinical-stage pipeline of next-gen Promising clinical data in heme TCR-T therapies • 2 of 26 (8%) treatment-arm subjects relapsed as • HEME PROGRAM: Targeting residual disease to prevent compared to 4 of 12 (33%) control-arm relapse in patients undergoing allogeneic HCT (1) subjects • SOLID TUMOR: Multiplex TCR-T therapy to • >$1B addressable market across US & EU overcome tumor heterogeneity and HLA loss In-house GMP manufacturing Multiple clinical catalysts supports early-stage development expected by end of 2025 HEME PROGRAM: • Global CDMO engaged to support late-stage • Launch pivotal study (2H25) clinical and commercial manufacturing • Present two-year relapse data on initial Ph1 patients (YE25) SOLID TUMOR: • Present safety and response data for multiplex TCR-T (1Q26) $218.0M as of June 30, 2025 funds operations into Q1 2027 (2) 129.8M total economic shares outstanding as of June 30, 2025 (1) As of latest data cut presented at ASH Annual Meeting in December 2024 (2) 3 Includes 56,747,993 outstanding common shares plus 73,087,945 pre-funded warrants

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![](g125738ex99_1p4g1.jpg)

TScan is building on the remarkable success of immunotherapy Checkpoint & TIL therapy TCR-T therapy CAR-T therapy Rejuvenating and expanding Engineering T cells to express Engineering T cells with a patient's existing T cells natural T cell receptors a synthetic receptor Proven efficacy in solid Promising efficacy in solid Poor solid tumor tumors tumors penetration Full range of targets seen by Full range of targets seen by Limited to cell surface immune system immune system antigens Most patients lack anti-cancer T T cells engineered with T cells engineered with cells and do not respond natural anti-cancer TCRs potent targeting receptors Limited applicability to heme Promising efficacy in heme Proven efficacy in heme malignancies to date malignancies malignancies 4

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![](g125738ex99_1p5g1.jpg)

TScan is targeting the most frequent human leukocyte antigens (HLAs) to address a broad patient population Everyone has six ~90% of people in the U.S. are class I HLAs positive for at least one of the \* top six HLA types Chromosome 6 Tumor cell Mother Father % people positive A A for each HLA type C C B B United HLA type Europe Asia States HLA class I (e.g., A\*02:01) A\*02:01 42 47 19 CD8a/b A\*01:01 24 26 14 A\*03:01 22 25 7.0 Target antigen B\*07:02 20 21 8.1 (e.g., PRAME) C\*07:02 24 23 24 TCR There are 100s of A\*24:02 17 19 37 different HLA types Most TCR-T companies only target one HLA (A\*02:01) TScan is developing a broad pipeline targeting the top six HLAs T cell \* allelefrequencies.net 5

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![](g125738ex99_1p6g1.jpg)

Nine TCR-T candidates in clinical development, with new TCR-Ts advancing Indications Programs (target) HLA type Discovery Lead optimization IND-enabling Phase 1 Phase 2/3 TSC-100 HLA-A\*02:01 (HA-1) AML, TSC-101 HLA-A\*02:01 HEMATOLOGIC MDS, (HA-2) MALIGNANCIES ALL HLA-A\*03:01 TSC-102 HLA-A\*01:01 (CD45) HLA-A\*24:02 HLA-A\*02:01 TSC-200 (HPV16) HLA-A\*01:01 HLA-B\*07:02 TSC-201 HLA-A\*02:01 (MAGE-C2) NSCLC, Sarcoma, SOLID TUMORS HLA-A\*02:01 TSC-202 Head & Neck, (T-PLEX) (MAGE-A4) HLA-A\*24:02 Cervical, Anal & Genital HLA-A\*02:01 TSC-203 (PRAME) HLA-A\*24:02 HLA-A\*02:01 TSC-204 HLA-C\*07:02 (MAGE-A1) HLA-A\*01:01 HLA-A\*03:01 AUTOIMMUNITY Crohn's 6

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![](g125738ex99_1p7g1.jpg)

Heme Malignancies: TSC-100, TSC-101, TSC-102-A0301 Targeting residual disease to prevent relapse in patients undergoing allogeneic HCT

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![](g125738ex99_1p8g1.jpg)

Relapse after hematopoietic cell transplant remains an unmet need AML, MDS, some ALL is not addressed by CAR-T due to shared antigens with normal blood cells Targeting antigens 38-44% of patients relapse mismatched between Allogeneic hematopoietic within two years following patients and donors can 1 cell transplant (HCT) RIC-Allo-HCT potentially prevent expected to remain relapse after allo-HCT standard of care Allo-HCT creates a unique Of those who opportunity to safely relapse, >80% target residual cancer cells while sparing normal die within blood cells 2 two years 1. CIBMTR analysis of AML, ALL, MDS allogeneic transplants with reduced intensity conditioning (RIC) between 2017-2019 with 2-year follow-up 8 2. Schmid, Blood 2012, Spyridonidis, Leukemia 2012, Schmid, Haematologica 2018

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![](g125738ex99_1p9g1.jpg)

TSC-100 and TSC-101 are bespoke allogeneic TCR-T cell therapies designed to eliminate residual cancer and prevent relapse following HCT TSC-101 PATIENT Patient journey for TSC-101 (HLA-A\*02:01-positive) HA-2 positive Residual Cancer HA-2 cancer Hematopoietic cell TCR-T cell cell negative transplant infusion HA-2 positive Normal blood cell HA-2 TCR Stem TSC-101 DONOR cells TSC-101 (HLA-A\*02:01-negative) Stem cell HA-2 negative TCR-T manufacturing T cell TSC-100 targets HA-1 TSC-101 targets HA-2 9

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![](g125738ex99_1p10g1.jpg)

ALLOHA , a multi-arm Phase 1 trial for TSC-100 & TSC-101 in subjects with AML, ALL, and MDS (NCT05473910) Control Arm Treatment Arms Transplant Transplant Transplant Alone + TSC-100 + TSC-101 (HA-1 positive) (HA-2 positive) Manufacturing TSC-100 or TSC-101 TSC-100 or TSC-101 apheresis Infusion 1 Infusion 2 Subject Donor HCT screening screening apheresis Treatment Arms Day 0 Day +21 Day +61 Control Arm Pre-transplant screening RIC HCT Endpoint and safety monitoring Key eligibility criteria Key endpoints • Age >18 years • Safety: Dose limiting toxicities, adverse events • Undergoing first allo-HCT for ALL, AML, MDS • Efficacy • Subject positive for HA-1 (or HA-2) with a haploidentical HA-1 (or HA-2) • Exploratory endpoints: Donor chimerism, minimal residual negative donor disease • Eligible for RIC-HCT followed by PTCy for GvHD prophylaxis 10 ALL, acute lymphoblastic leukemia; AML, acute myeloid leukemia; MDS, myelodysplastic syndromes; GvHD, graft vs host disease; RIC-HCT, reduced intensity conditioning hematopoietic cell transplantation

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![](g125738ex99_1p11g1.jpg)

Majority of subjects in the treatment and control arms are at high risk for relapse TSC-100 TSC-101 Any TSC Control Subjects Enrolled and Assigned 14 12 26 13 Subjects Transplanted (efficacy data cohort) 14 12 26 12 Subjects Infused (safety data cohort) 10 12 22 N/A\* Median Time of Follow Up, months 4.0 (0-19) 6.4 (1-21) 5.1 (0-21) 7.1 (1-25) Age, Median (Range) 69 (39-76) 66 (52-74) 67 (39-76) 66 (23-74) Sex, Male (n, %) 10 (71%) 7 (58%) 17 (65%) 6 (46%) ALL 2 (14%) 2 (17%) 4 (15%) 0 (0%) Underlying Disease AML 10 (71%) 7 (58%) 17 (65%) 8 (62%) MDS 2 (14%) 3 (25%) 5 (19%) 5 (38%) TP53 Mutated 4 (29%) 2 (17%) 6 (23%) 2 (15%) Genetics/Cytogenetics FLT3 Mutation 2 (14%) 0 (0%) 2 (8%) 5 (38%) Adverse Risk\*\* 11 (79%) 10 (83%) 21 (81%) 8 (62%) Pre-HCT MRD Positive\*\*\* 8 (57%) 5 (42%) 13 (50%) 7 (54%) MRD Positive or Adverse Risk Genetics 11 (79%) 10 (83%) 21 (81%) 10 (77%) \*Control subjects that received transplant are included in the safety data cohort \*\*Adverse risk is defined as having either a IPSS-M mutation if the subject has MDS or European Leukemia Network (ELN) high risk genetics or cytogenetics for AML; ELN 2022 high risk genetics/ cytogenetics include mutated ASXL1, BCOR, EZH2, RUNX1, SF3B1, SRSF2, STAG2, U2AF1, ZRSR2, TP53, -5/ del(5q)/, -7,-17/ abn(17p), t(6;9), t(v;11q23.3), t(9;22), t(8;16), inv(3) or t(3;3), t(3q26.2;v), monosomal or complex karyotype (for AML); IPSS-M mutations are reported in Bernard et al, NEJM Evid, 2022 (for MDS) \*\*\*MRD measured by flow cytometry (lower limit of detection 0.1-1%) or NGS (lower limit of detection 0.05-0.1% in myeloid and 0.001-0.01% in lymphoid malignancies). 11 As of latest data cut presented at ASH Annual Meeting December 2024

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![](g125738ex99_1p12g1.jpg)

No dose-limiting toxicities observed at all three dose levels in subjects treated with TSC-100 or TSC-101 Planned Day of Infusion Post HCT TSC 100 TSC 101 Dose Level N=10 N=12 +21 +61 6 DL1 5×10 TCR-T cells/kg N/A 1 1 6 6 DL2 5×10 TCR-T cells/kg 5×10 TCR-T cells/kg 1 2 6 6 DL3 5×10 TCR-T cells/kg 20×10 TCR-T cells/kg 8 9 12 As of latest data cut presented at ASH Annual Meeting December 2024

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![](g125738ex99_1p13g1.jpg)

TSC-100 and TSC-101 TCR-T cells detected for over one year with increased persistence seen at highest dose level (DL3) TSC persistence over time TSC-100 TSC-101 15 15 15 Dose Level 1 Dose Level 2 Dose Level 3 10 10 10 AUC 2.26\* AUC 8.15\* AUC 34.47\* 5 5 5 0.2 0.2 0.2 0.1 0.1 0.1 0.0 0.0 0.0 0 50 100 150 200 250 300 350 400 0 50 100 150 200 250 300 350 400 0 50 100 150 200 250 300 350 400 Days post TSC-infusion Days post TSC-infusion Days post TSC-infusion Infusion 1 Infusion 1 Infusion 2 Infusion 1 Infusion 2 \*AUC of TSC-100/TSC-101 between Day 90-180 (Geometric mean(geometric CV)): DL1: 2.26(47.2%); DL2 and sDL2: 8.15(42.2%); DL3 and sDL3: 13 34.47(97.7%). Dose did not meet target dose criteria in supplemental dose level cohorts (sDL) As of latest data cut presented at ASH Annual Meeting December 2024 TSC-100/TSC-101 - % of peripheral T cells TSC-100/TSC-101 - % of peripheral T cells TSC-100/TSC-101 - % of peripheral T cells

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![](g125738ex99_1p14g1.jpg)

Adverse events of special interest are low grade and manageable TSC-101 Any TSC TSC-100 Control Adverse Event of Special Interest\* n=10 n=12 n=22 n=12 • Balanced Grade II – IV acute GvHD between Any Acute GvHD\*\* 5 (50%) 6 (50%) 11 (50%) 4 (33%) treatment and control arms Grade II - IV 0 (0%) 2 (17%) 2 (9%) 3 (25%) • No cases of moderate or severe chronic GvHD Grade III - IV 0 (0%) 1 (8%) 1 (5%) 2 (17%) • One case each of mild chronic GvHD in the treatment and control arms Any CRS 4 (40%) 8 (67%) 12 (57%) 6 (50%) Grade 1 - 2 4 (40%) 8 (67%) 12 (57%) 6 (50%)• Two episodes of low-grade CRS reported post TSC infusions Grade 3 - 4 0 (0%) 0 (0%) 0 (0%) 0 (0%) • One Grade 1 event (TSC-100) and one Treatment-emergent CRS 1 (10%) 1 (8%) 2 (9%) NA Grade 2 event (TSC-101) Grade 1 - 2 1 (10%) 1 (8%) 2 (9%) NA • No cases of ICANS Grade 3 - 4 0 (0%) 0 (0%) 0 (0%) NA Any ICANS 0 (0%) 0 (0%) 0 (0%) 0 (0%) \*MAGIC grading used for acute GvHD, NIH consensus grading for chronic GvHD, and ASTCT grading used for CRS or ICANS \*\*Acute GvHD through 180 days post HCT 14 As of latest data cut presented at ASH Annual Meeting December 2024

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![](g125738ex99_1p15g1.jpg)

Key biomarkers for residual leukemia or residual patient-derived blood cells serve as potential early surrogates of efficacy Post-transplant Patient Minimal Residual Disease Donor Chimerism (MRD) MRD+: high risk of relapse Mixed: high risk of relapse 1, 2 3 MRD-: low risk of relapse Complete: low risk of relapse Residual Residual blood cells Leukemia (HA-1-positive) (HA-1-positive) HA-1-negative donor blood cells 1. Craddock, J Clin Oncol, 2021 3. Lindhal, Bone Marrow Transpl, 2022 2. Loke, ASH, 2021 15

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![](g125738ex99_1p16g1.jpg)

Complete donor chimerism achieved in all patients after initial TSC infusion TSC-100/TSC-101 Treatment-arm subjects Control-arm subjects Infused with TCR-T cells 101 100 101 100 101 100 101 100 101 100 101 101 101 100 100 101 100 101 100 100 100 100 C 1 C 2 C 3 C 4 C 5 C 6 C 7 C 8 C 9 C 10 C 11 C 12 ‡ ‡ DL1 DL1 DL2s DL2 DL2 DL3 DL3s DL3 DL3 DL3 DL3 DL3 DL3 DL3 DL3 DL3 DL3 DL3 DL3 DL3 n/a n/a Time post MDS T-ALL AML AML B-ALL AML B-ALL MDS MDS AML AML AML MDS AML AML AML AML AML B-ALL MDS AML AML MDS MDS MDS AML AML AML AML AML AML MDS MDS AML HCT Day X 21/28 Day 42 Day 56 Day X X 77 Relapse Day 105 Day X 133 Relapse Relapse Day X 161 Relapse Day 228 Relapse Day X 318 Day X 388 Mths X 14-24 Clinical intervention TSC-100/101 Complete donor Mixed donor Death unrelated to for increasing mixed Relapse Death from relapse X X Infusion chimerism chimerism relapse or TSC chimerism 16 Donor chimerism results using commercially available short tandem repeat (STR) assay with LOD of 1-2% at indicated times post- As of latest data cut presented at ASH Annual Meeting December 2024 HCT ± 3 days in patients at least 60 days post-HCT as of data cut; ‡Dose did not meet target dose criteria in supplemental cohorts

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![](g125738ex99_1p17g1.jpg)

MRD negativity achieved in all treatment-arm subjects Pre-HCT 6 months 12 months 24 months Arm Disease MRD P-004-0001 TSC-101 DL 1 MDS p53m P-004-0004 TSC-100 DL1 T-ALL X ‡ TSC-101 DL2s AML P-004-0005 P-007-0002 TSC-100/TSC-101 infusion TSC-100 DL2 AML P-004-0006 TSC-101 DL2 B-ALL Clinical intervention for AML P-004-0007 TSC-100 DL3 ‡ concern for relapse B-ALL P-004-0008 TSC-101 DL3s TSC-100 DL3 P-006-0003 MDS X Relapse P-023-0001 TSC-101 DL3 MDS P-013-0002 TSC-100 DL3 AML AML P-006-0005 TSC-101 DL3 X Death from relapse AML P-004-0011 TSC-101 DL3 TSC-101 DL3 MDS P-007-0005 Death unrelated to X AML p53m P-013-0004 TSC-100 DL3 relapse or TSC P-025-0001 TSC-100 DL3 AML P-013-0003 TSC-101 DL3 AML X Ongoing follow-up Treatment arm AML P-007-0004 TSC-100 DL3 End of study TSC-101 DL3 P-013-0005 AML p53m Median time to relapse P004-0013 TSC-100 DL3 B-ALL p53m MRD-positive MDS P-007-0007 TSC-100 DL3 has not been reached MRD-negative P-004-0014 TSC-101 DL3 AML AML P013-0006 TSC-101 DL3 MRD pending TSC-100 DL3 AML P025-0002 P004-0015 TSC-100 DL3 AML p53m ‡Dose did not meet target P-023-0002 TSC-100 not dosed AML X dose criteria in supplemental P-018-0003 X TSC-100 not dosed AML p53m MDS P-002-0001 cohorts Control-1 MDS P-007-0001 Control-2 P-006-0001 Control-3 MDS p53m X MRD measured by flow Control arm P-006-0002 Control-4 AML cytometry (lower limit of P-018-0001 Control-5 AML detection 0.1-1%) or NGS Median time to relapse AML P-002-0002 Control-6 (lower limit of detection 0.05- Control-7 AML P-004-0010 X is 160 days P-002-0003 0.1% in myeloid and 0.001- Control-8 AML p53m X P-018-0004 Control-9 AML 0.01% in lymphoid P-004-0012 Control-10 MDS malignancies). Control-11 MDS P-006-0007 Control-12 AML P-020-0001 0 100 200 300 400 500 600 700 Days post-HCT 17 As of latest data cut presented at ASH Annual Meeting December 2024

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![](g125738ex99_1p18g1.jpg)

TCR-T infusion is associated with fewer relapses Control arm 4 of 12 (33%) 0.40 0.30 0.20 Treatment arm 2 of 26 (8%) 0.10 HR = 0.28 0.00 0 45 90 135 180 225 270 315 360 405 450 495 540 585 630 675 720 765 Days post HCT Number at risk Control arm 12 11 9 9 5 4 4 4 4 2 2 1 1 1 1 1 1 1 Treatment arm 26 23 17 15 12 10 8 8 8 7 6 4 3 3 1 0 0 0 Cumulative number of events Control arm 0 0 1 1 3 4 4 4 4 4 4 4 4 4 4 4 4 4 Treatment arm 0 0 0 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 18 CoxPH Ratio = 0.275, CI = (0.05, 1.502), p = 0.136; Log-rank p = 0.1105 As of latest data cut presented at ASH Annual Meeting December 2024 Probability of relapse

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![](g125738ex99_1p19g1.jpg)

Event-free survival (EFS) favors the treatment arm 1.00 Treatment arm 0.75 6 of 26 (23%) 0.50 Control arm 7 of 12 (58%) 0.25 HR = 0.30 0.00 0 60 120 180 240 300 360 420 480 540 600 Days post HCT Number at risk Control arm 12 11 7 3 2 2 2 0 0 0 0 Treatment arm 26 23 17 12 10 8 8 7 4 3 1 Cumulative number of events Control arm 0 1 4 6 7 7 7 7 7 7 7 Treatment arm 0 1 2 4 4 4 4 5 6 6 6 Event defined as relapse, clinical intervention for impending relapse (non-TSC), or death 19 Cox PH Ratio = 0.304, CI = (0.096, 0.966, p = 0.0435); Log-rank p = 0.0321 As of latest data cut presented at ASH Annual Meeting December 2024 EFS probability

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![](g125738ex99_1p20g1.jpg)

ALLOHA Phase 1 data support launch of pivotal trial in H2 2025 • Infusions with TSC-100 and TSC-101 were well-tolerated with no DLTs and adverse events consistent with HCT • TSC-100 and TSC-101 TCR-T cells have been detected >1 year post infusion and have a clear dose- persistence relationship • 2 of 26 (8%) treatment-arm subjects relapsed as compared to 4 of 12 (33%) control-arm subjects - Median time to relapse was not evaluable in TCR-T-treated subjects vs 160 days in the control arm - Event-free survival strongly favors the treatment arm (HR=0.30) 20 As of latest data cut presented at ASH Annual Meeting December 2024

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![](g125738ex99_1p21g1.jpg)

Heme Development Strategy Targeting residual disease to prevent relapse in patients undergoing allogeneic HCT

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![](g125738ex99_1p22g1.jpg)

TSC-101 captures ~98% of HLA-A\*02:01-positive patients, obviating the need for TSC-100 or a companion diagnostic 100 Almost all subjects infused to date [19/22 (86.3%)] 90 qualified for treatment with TSC-101 2.5% 80 70 60 TSC-100 TSC-101 50 (22.7%) (33.6%) 40 30 20 10 0 0 10 20 30 40 50 60 % Eligible patients 22 Sources: Wang, AACR 2022; Spierings, PLoS Genetics 2007; CIBMTR 2023 % Eligible donors

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Pivotal study designed for full approval using an external control arm Subjects: AML, MDS, ALL undergoing transplant with reduced intensity conditioning (RIC) Donors: Haploidentical and mismatched unrelated donors Enrollment: TSC-101 vs matched controls (1:3) Companion Diagnostic: Not needed Treatment arm Synthetic control arm (HLA-A\*02:01, HA-2 positive) CIBMTR RIC Transplant alone RIC Transplant matched to TSC-101 + TSC-101 subjects Endpoints Readouts • Primary: Relapse-free survival (RFS; Full • Full Approval: 184 relapse + death events approval)• HR 0.60, 85% power • Key Secondary: Overall survival, time to relapse, • N = ~140 treatment arm subjects event-free survival• Study Readout: 24 months • Exploratory: MRD, complete chimerism rates 23

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Target RFS hazard ratio of 0.60 is well supported by data from the ALLOHA Phase 1 study 1.00 TSC-101 1 of 12 (17%) 0.75 Control arm 5 of 12 (42%) 0.50 0.25 HR = 0.20 0.00 0 45 90 135 180 225 270 315 360 405 450 495 540 585 630 675 720 765 Days post HCT Number at risk Control arm 12 11 9 9 5 4 4 4 4 2 2 1 1 1 1 1 1 1 Treatment arm 12 11 9 8 7 5 4 4 4 4 3 3 2 2 1 0 0 0 Cumulative number of events Control arm 0 1 2 2 4 5 5 5 5 5 5 5 5 5 5 5 5 5 Treatment arm 0 0 0 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Event defined as relapse, or death 24 CoxPH Ratio = 0.2, CI = (0.023, 1.718), p = 0.1425; Log-rank p = 0.1034 As of post-ASH KOL call in December 2024 RFS probability

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Increased use of reduced intensity conditioning with haploidentical/MMUD donors has the potential to dramatically expand the addressable market Addressable TSC-101 patients in the U.S. and EU 6,000 KOLs anticipate increased use of KOLs also anticipate increased use of haplo and/or MMUD transplants RIC over MAC driven by improved 5,000 over traditional MUD transplants to relapse rates and decreased risk of MAC to RIC enable on-label use of TSC-101, treatment-related mortality 4,000 driven by improved relapse rates over standard of care 3,000 MUD to Haplo/MMUD MUD to Haplo/MMUD 2,000 1,000 Current practice Current practice Current practice 0 Current practice Anticipated change in donor Anticipated change in conditioning selection practice regimen practice If successful, use of TSC-101 will drop relapse rates relative to all other types of transplants Sources: CIBMTR 2022 and 2023; Wang, AACR 2022; NMDP analysis; ClearView analysis. 25 MUD, matched unrelated donor; Haplo, haploidentical transplant; MMUD, Assumes maximum practice change in each case and extrapolation to 2033 as peak of practice change mismatched unrelated donor. # of addressable patients

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TCRs for additional HLA types will target epitopes on CD45, a universal source of antigens for heme malignancies • CD45 is a lineage-specific antigen with expression in all hematopoietic cells, including HSCs • CD45 is a large protein with many well-known epitopes for high frequency HLAs • Antigen-negative donors can be selected by mismatching on HLA (using haploidentical and MMUD donors) CD45 has high and uniform TSC-102 targets an antigen from CD45 expression in AML and ALL presented on HLA-A\*03:01 U937 AML ALL U937 E (My :T o el f o 5id le :1 ukemia) 800 CD45 CD45 600 10 400 Non-engineered T cells 200 8 6 100 4 2 50 0 HA-1 HA-2 HA-1 HA-2 -2 TSC-102 0 Source: TCGA 0 20 40 60 80 E:T of 5:1 Time (hour) 26 log (TPM+0.001) 2 Cell Survival (%)

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Expansion opportunities for the heme program provide a way to reach over 10,000 patients in the U.S. and Europe 20,000 Addition of other heme malignancies 15,000 Addition of frail and R/R patients Addition of three TCRs for other HLA types 10,000 TSC-101 (assumes change in practice) 5,000 0 Current program HLA expansion Transplant expansion Disease expansion Source: SEER, CIBMTR, EBMT, ClearView analysis 27 # of addressable patients

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Solid Tumors: TSC-200-A0201 TSC-204-A0201 TSC-201-B0702 TSC-204-C0702 TSC-202-A0201 TSC-204-A0101 TSC-203-A0201 Developing multiplex TCR-T therapy to overcome tumor heterogeneity

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Multiplex TCR-T therapy is designed to address the heterogeneity of solid tumors Many immune-rich cancers exhibit TCR-Ts against multiple targets may be target heterogeneity required to improve efficacy and durability Non-small cell lung cancer PRAME MAGE-A4 PRAME MAGE-A4 Singleplex Multiplex TCR-T TCR-T for PRAME for PRAME + MAGE-A4 Targeting long-term Relapse remission or cure 29

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TScan's solution for combatting the hostile tumor microenvironment Tumor cell Enhanced TCR-T enabled TCR by TScan's transposon- CD8a/b CD8a/b TCR based manufacturing CD4 Cytotoxic DN-TGFbRII platform Helper T cell T cell TGFb IL-2 Cytokine support • Co-deliver CD8a/b to engage helper T-cells • Co-deliver DN-TGFbRII to enhance T-cell expansion/persistence 30

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TScan is building the ImmunoBank of TCRs to enable multiplex TCR-T cell therapy Cancer patient ImmunoBank of therapeutic TCRs Customized TCR-T therapy HLAs T cells HLA Type Profile tumor Cancer cells with different HLA types and targets • Determine target and HLA expression in patient tumor • Manufacture and administer customized, multiplex TCR-T therapy 31 Targets

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TScan is strategically expanding the ImmunoBank to enable multiplex TCR-T therapy in immune-rich solid tumors HLAs HPV16 TSC-200 MAGE-C2 TSC-201 In PLEXI-T study MAGE-A4 TSC-202 PRAME TSC-203 MAGE-A1 TSC-204 ImmunoBank 32 Targets A\*02:01 A\*01:01 A\*03:01 B\*07:02 C\*07:02 A\*24:02

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PLEXI-T , a multi-arm basket Phase 1 trial in subjects with solid tumors (NCT05973487) Screening Protocol Treatment Protocol Tumor testing Germline TCR-T cell manufacturing for target followed by HLA testing and infusion testing for HLA loss Patient selection Subject Tumor Infusion 1 Infusion 2 screening testing Manufacturing apheresis Screening Protocol Day -6 Day +1 Day +29 Day +753 Treatment Protocol Eligibility screening LD Endpoint and safety monitoring Long term follow-up Key eligibility criteria Key endpoints • Age >18 years • Safety: Dose limiting toxicities, adverse events • Relapsed/refractory solid tumor after treatment with or refusal of SoC • Efficacy therapies • Exploratory: T cell persistence • Eligible for treatment on a Phase 1 study that requires lymphodepleting chemotherapy 33 LD: lymphodepletion with fludarabine x 4 days and cyclophosphamide x 3 days; SoC: standard of care therapy; HLA: human leukocyte antigen

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Dose escalation scheme provides a path to multiplex TCR-T in Phase 1 TSC-200-A0201 TSC-201-B0702 TSC-202-A0201 TSC-203-A0201 TSC-204-A0201 TSC-204-C0702 TSC-204-A0101 (HPV16) (MAGE-C2) (MAGE-A4) (PRAME) (MAGE-A1) (MAGE-A1) (MAGE-A1) 0.5B 0.5B 0.5B 0.5B 0.5B 0.5B 0.5B DL1 Cleared Dosed Cleared Cleared Cleared 2B 2B 2B 2B 2B 2B 2B DL2 Cleared Cleared Cleared T-Plex Singleplex Any two TCR-Ts that 28 days 28 days + + DL3 have cleared DL2 First infusion First infusion Second infusion Second infusion Any two TCR-Ts that 28 days 28 days DL4 + + have cleared DL3 First infusion First infusion Second infusion Second infusion 34 As of Sept 8, 2025

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T-Plex enrollment focuses on five key indications with high unmet need Potential U.S. Indication patients ~63 K NSCLC Sarcoma PRAME + MAGE-A1/C2/A4 ~3 K Head & Neck ~15 K HPV16 + PRAME Cervical ~4 K HPV16 + MAGE-A1/C2/A4 Anal & Genital ~7 K • High unmet need • Evidence of T cell infiltration • Clinical signal in early TCR-T trials • Significant addressable patient population in second- and third-line treatment Sources: SEER; KOL research; Kantar; Chao X, Song X, Wu H, You Y, Wu M and Li L (2021) Selection of Treatment Regimens for 35 Recurrent Cervical Cancer. Front. Oncol. 11:618485. doi: 10.3389/fonc.2021.618485

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ImmunoBank expansion to seven TCRs has increased T-Plex eligibility across target indications Patients eligible for multiplex TCR-T cell therapy in PLEXI-T study 60% 60% 50% 15-60% of patients are 40% currently eligible for 40% multiplex TCR-T cell 30% 30% therapy with the 7 25% TCRs in the current 20% 15% PLEXI-T study 10% 0% NSCLC Sarcoma Head & Cervical Anal & Neck Genital \*Eligible patients include patients who are positive for at least two TCR-Ts in the ImmunoBank 36 \* Eligible patients (%)

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Autoimmunity represents an exciting area of unmet need with few validated targets • Most current therapies provide general immune suppression, leading to complications (e.g., increased risk of infection) • Target-specific therapies provide a way to address the cause, rather than the symptoms, of autoimmunity • Many AI disorders have a substantial T-cell component, but the targets of these pathogenic or protective T-cells are largely unknown • TScan's target discovery platform provides a way to identify targets in autoimmune disease, unlocking the development of targeted therapeutics Multi-year collaboration using TargetScan to identify targets for T cells in patients with Crohn's disease Recently identified targets for other autoimmune disorders using proprietary platform; plan to share initial internal data at a medical meeting in H2 2025 37

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Steady value-generating data flow planned across clinical programs H2 2025 Q1 2026 YE 2025 Launch pivotal study Two-year relapse data from Initial safety and response for TSC-101 initial patients (TSC-101) data for multiplex TCR-T File IND for TSC-102-A0301 (CD45, HLA-A\*03:01) Present internal autoimmune data Heme Program Solid Tumor Program Autoimmune Program 38

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TScan is a fully integrated, next-generation TCR-T therapy company Clinical-stage pipeline of next-gen Promising clinical data in heme TCR-T therapies • 2 of 26 (8%) treatment-arm subjects relapsed as • HEME PROGRAM: Targeting residual disease to prevent compared to 4 of 12 (33%) control-arm relapse in patients undergoing allogeneic HCT (1) subjects • SOLID TUMOR: Multiplex TCR-T therapy to • >$1B addressable market across US & EU overcome tumor heterogeneity and HLA loss In-house GMP manufacturing Multiple clinical catalysts supports early-stage development expected by end of 2025 HEME PROGRAM: • Global CDMO engaged to support late-stage • Launch pivotal study (2H25) clinical and commercial manufacturing • Present two-year relapse data on initial Ph1 patients (YE25) SOLID TUMOR: • Present safety and response data for multiplex TCR-T (1Q26) $218.0M as of June 30, 2025 funds operations into Q1 2027 (2) 129.8M total economic shares outstanding as of June 30, 2025 (1) As of latest data cut presented at ASH Annual Meeting - December 2024 (2) 39 Includes 56,747,993 outstanding common shares plus 73,087,945 pre-funded warrants

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THANK YOU