# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001193125-25-301809
**Filing Date:** 2025-11
**Character Count:** 36158
**Document Hash:** c50f4d01b545511bfb8934cd82fd16a1
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-301809.hdr.sgml**: 20251128

**ACCESSION NUMBER**: 0001193125-25-301809

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250930

**FILED AS OF DATE**: 20251128

**DATE AS OF CHANGE**: 20251128

**PERIOD START**: 20250930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 251534215

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Research Fund (Series ID: S000010470)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028926 | Class T      | JAMRX           |
| C000077669 | Class A      | JRAAX           |
| C000077670 | Class C      | JRACX           |
| C000077671 | Class I      | JRAIX           |
| C000077672 | Class S      | JRASX           |
| C000083517 | Class D      | JNRFX           |
| C000114872 | Class N      | JRANX           |
| C000177053 | Class R      | JRARX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Research Fund

- **b. EDGAR series identifier (if any):** S000010470

- **c. LEI of Series:** TO5UP72VAT80SBB47C78

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-09-30

- **b. Date as of which information is reported:** 2025-09-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $28617989217.95

**Total Liabilities:** $21918433.53

**Net Assets:** $28596070784.42

**Cash Not Reported:** $3540234.37

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028926 | 3.85%                | -0.18%               | 3.82%                |
| Class ID C000077669 | 3.83%                | -0.18%               | 3.81%                |
| Class ID C000077670 | 3.78%                | -0.26%               | 3.75%                |
| Class ID C000077671 | 3.86%                | -0.16%               | 3.84%                |
| Class ID C000077672 | 3.81%                | -0.20%               | 3.80%                |
| Class ID C000083517 | 3.85%                | -0.17%               | 3.83%                |
| Class ID C000114872 | 3.87%                | -0.16%               | 3.84%                |
| Class ID C000177053 | 3.80%                | -0.22%               | 3.77%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $87417493.00             | $939167160.00                              |
| Month 2  | $70238046.00             | $-113057029.00                             |
| Month 3  | $432572655.00            | $631837155.00                              |

**Designated Index Information**

- **Index Name:** Russell 1000 Growth Total Return Index

- **Index Identifier:** RUSSELL.MC.RLG

### Schedule of Portfolio Investments

| Name                                         | Title                                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)    | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Cadence Design Systems, Inc.                 | Cadence Design Systems, Inc. COM USD0.01                              | CUSIP: 127387108<br>LEI: GCT7RXJOGLXPV0NXZY22 | Long             | EC               | CORP              | US        |    706262 | NS      | $248081590.12  | 0.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Capital One Financial Corp.                  | Capital One Financial Corp. COM USD0.01                               | CUSIP: 14040H105<br>LEI: ZUE8T73ROZOF6FLBAR73 | Long             | EC               | CORP              | US        |    427556 | NS      | $90889854.48   | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Brands, Inc.                   | Constellation Brands, Inc. COM USD0.01 CLASS A                        | CUSIP: 21036P108<br>LEI: 5493005GKEG8QCVY7037 | Long             | EC               | CORP              | US        |    440113 | NS      | $59270017.71   | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                          | Danaher Corporation COM USD0.01                                       | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |    231702 | NS      | $45937238.52   | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| TFI International Inc                        | TFI International Inc COM                                             | CUSIP: 87241L109<br>LEI: 5493007R9W7KO7SJN678 | Long             | EC               | CORP              | US        |   1159788 | NS      | $102130931.28  | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| The TJX Companies Inc.                       | The TJX Companies Inc. COM USD1                                       | CUSIP: 872540109<br>LEI: V167QI9I69W364E2DY52 | Long             | EC               | CORP              | US        |   1971899 | NS      | $285018281.46  | 1.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Co., Ltd. | Taiwan Semiconductor Manufacturing Co., Ltd. SPON ADS REP 5 ORD TWD10 | CUSIP: 874039100<br>LEI: 549300KB6NK5SBD14S87 | Long             |  | CORP              | US        |    394016 | NS      | $110044728.64  | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Tesla Inc                                    | Tesla Inc COM USD0.001                                                | CUSIP: 88160R101<br>LEI: 54930043XZGB27CTOV49 | Long             | EC               | CORP              | US        |    448365 | NS      | $199396882.80  | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| 3M Company                                   | 3M Company COM USD0.01                                                | CUSIP: 88579Y101<br>LEI: LUZQVYP4VS22CLWDAR65 | Long             | EC               | CORP              | US        |   1749283 | NS      | $271453735.94  | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                  | AbbVie Inc. COM USD0.01                                               | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |    522735 | NS      | $121034061.90  | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Alphabet Inc.                                | Alphabet Inc. CAP USD0.001 CL C                                       | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |   4932552 | NS      | $1201323039.60 | 4.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Amazon.com, Inc.                             | Amazon.com, Inc. COM USD0.01                                          | CUSIP: 023135106<br>LEI: ZXTILKJKG63JELOEG630 | Long             | EC               | CORP              | US        |   6003288 | NS      | $1318141946.16 | 4.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                                   | Amgen Inc. COM USD0.0001                                              | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |    415752 | NS      | $117325214.40  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation              | United Therapeutics Corporation COM USD0.01                           | CUSIP: 91307C102<br>LEI: 5299005C4HZL4UWROC14 | Long             | EC               | CORP              | US        |     31175 | NS      | $13068871.75   | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                                  | Vaxcyte Inc COM                                                       | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |   1010488 | NS      | $36397777.76   | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated          | Vertex Pharmaceuticals Incorporated COM USD0.01                       | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |    399761 | NS      | $156562398.04  | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Visa Inc                                     | Visa Inc COM STK USD0.0001                                            | CUSIP: 92826C839<br>LEI: 549300JZ4OKEHW3DPJ59 | Long             | EC               | CORP              | US        |   1833587 | NS      | $625949930.06  | 2.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Vistra Corp                                  | Vistra Corp COM USD0.01                                               | CUSIP: 92840M102<br>LEI: 549300KP43CPCUJOOG15 | Long             | EC               | CORP              | US        |    730132 | NS      | $143047461.44  | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Wayfair Inc                                  | Wayfair Inc COM USD0.001 A                                            | CUSIP: 94419L101<br>LEI: 54930027N9ZAVYFDHK53 | Long             | EC               | CORP              | US        |    370171 | NS      | $33067375.43   | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Amphenol Corporation                         | Amphenol Corporation CL'A'COM USD0.001                                | CUSIP: 032095101<br>LEI: 549300D3L3G0R4U4VT04 | Long             | EC               | CORP              | US        |   2005913 | NS      | $248231733.75  | 0.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Analog Devices Inc.                          | Analog Devices Inc. COM USD0.16 2/3                                   | CUSIP: 032654105<br>LEI: GYVOE5EZ4GDAVTU4CQ61 | Long             | EC               | CORP              | US        |    456097 | NS      | $112063032.90  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Apollo Global Management Inc                 | Apollo Global Management Inc COM                                      | CUSIP: 03769M106<br>LEI: 254900LMFT1CY9IYB476 | Long             | EC               | CORP              | US        |    750957 | NS      | $100080039.39  | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                                   | Apple Inc. COM USD0.00001                                             | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |   7396641 | NS      | $1883406697.83 | 6.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Wingstop Inc                                 | Wingstop Inc COM USD0.01                                              | CUSIP: 974155103<br>LEI: 549300SSH3F8HPIM3Q47 | Long             | EC               | CORP              | US        |    146948 | NS      | $36983872.64   | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Workday Inc                                  | Workday Inc COM USD0.001 CL A                                         | CUSIP: 98138H101<br>LEI: 549300J0DYC0N31V7G13 | Long             | EC               | CORP              | US        |    294849 | NS      | $70978999.77   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Applovin Corp.                               | Applovin Corp. COM CL A                                               | CUSIP: 03831W108<br>LEI: 549300LLVXMUAOL3SQ07 | Long             | EC               | CORP              | US        |    469475 | NS      | $337336566.50  | 1.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Ares Management Corporation                  | Ares Management Corporation COM USD0.01 CLASS A                       | CUSIP: 03990B101<br>LEI: 529900JUV61FH7B8VW38 | Long             | EC               | CORP              | US        |    673411 | NS      | $107671684.79  | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                    | Argenx SE SPON ADR EACH REP 1 ORD SHS                                 | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    133808 | NS      | $98691428.48   | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Arista Networks Inc                          | Arista Networks Inc COM SHS                                           | CUSIP: 040413205<br>LEI: 635400H1WKBLOQERUU95 | Long             | EC               | CORP              | US        |    971992 | NS      | $141628954.32  | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                              | AstraZeneca PLC SPON ADR EACH REP 0.50 ORD                            | CUSIP: 046353108<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             |  | CORP              | US        |    600185 | NS      | $46046193.20   | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlassian Corporation                        | Atlassian Corporation CL A                                            | CUSIP: 049468101<br>LEI: 549300V7ZY5P02D2MY38 | Long             | EC               | CORP              | US        |    632771 | NS      | $101053528.70  | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Autodesk Inc                                 | Autodesk Inc COM USD0.01                                              | CUSIP: 052769106<br>LEI: FRKKVKAIQEF3FCSTPG55 | Long             | EC               | CORP              | US        |    847789 | NS      | $269317131.63  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.                     | Avidity Biosciences Inc. COM                                          | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1095488 | NS      | $47730412.16   | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Blackstone Inc                               | Blackstone Inc COM USD0.00001 CLASS A                                 | CUSIP: 09260D107<br>LEI: 5299004LW4QWGZUB8Y96 | Long             | EC               | CORP              | US        |   1185642 | NS      | $202566935.70  | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| The Boeing Company                           | The Boeing Company COM USD5.00                                        | CUSIP: 097023105<br>LEI: RVHJWBXLJ1RFUBSY1F30 | Long             | EC               | CORP              | US        |    720009 | NS      | $155399542.47  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                         | Booking Holdings Inc COM USD0.008                                     | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |     49703 | NS      | $268359916.81  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation                | Boston Scientific Corporation COM USD0.01                             | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |    918073 | NS      | $89631466.99   | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadcom Inc                                 | Broadcom Inc COM USD0.001                                             | CUSIP: 11135F101<br>LEI: 549300WV6GIDOZJTV909 | Long             | EC               | CORP              | US        |   5031596 | NS      | $1659973836.36 | 5.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Datadog Inc                                  | Datadog Inc COM USD0.00001 CL A                                       | CUSIP: 23804L103<br>LEI: 549300F6JNO0KRPO1K63 | Long             | EC               | CORP              | US        |   1437986 | NS      | $204769206.40  | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Deere & Company                              | Deere & Company COM USD1                                              | CUSIP: 244199105<br>LEI: PWFTNG3EI0Y73OXWDH08 | Long             | EC               | CORP              | US        |    266782 | NS      | $121988737.32  | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| DexCom Inc                                   | DexCom Inc COM USD0.001                                               | CUSIP: 252131107<br>LEI: 549300YSK3QDSFR5EU59 | Long             | EC               | CORP              | US        |    916517 | NS      | $61672428.93   | 0.22%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                                 | DoorDash Inc CL A                                                     | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |    681079 | NS      | $185246677.21  | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Ecolab Inc.                                  | Ecolab Inc. COM USD1                                                  | CUSIP: 278865100<br>LEI: 82DYEISM090VG8LTLS26 | Long             | EC               | CORP              | US        |    527601 | NS      | $144488809.86  | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Meta Platforms Inc                           | Meta Platforms Inc COM USD0.000006 CL 'A'                             | CUSIP: 30303M102<br>LEI: BQ4BKCS1HXDV9HN80Z93 | Long             | EC               | CORP              | US        |   1960046 | NS      | $1439418581.48 | 5.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson Enterprises Inc                     | Ferguson Enterprises Inc COMMON STOCK NEW                             | CUSIP: 31488V107<br>LEI: 2138003JYQMRP3SLX189 | Long             | EC               | CORP              | US        |    526820 | NS      | $118313235.60  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Gallagher Aj &Co                             | Gallagher Aj &Co COM USD1.00                                          | CUSIP: 363576109<br>LEI: 54930049QLLMPART6V29 | Long             | EC               | CORP              | US        |    345039 | NS      | $106872379.86  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| GE Aerospace                                 | GE Aerospace COM NEW                                                  | CUSIP: 369604301<br>LEI: 3C7474T6CDKPR9K6YT90 | Long             | EC               | CORP              | US        |   1347186 | NS      | $405260492.52  | 1.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Hilton Worldwide Holdings Inc                | Hilton Worldwide Holdings Inc COM USD0.01                             | CUSIP: 43300A203<br>LEI: 549300HVGPK36ICB0B89 | Long             | EC               | CORP              | US        |    721952 | NS      | $187303226.88  | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Howmet Aerospace Inc                         | Howmet Aerospace Inc COM                                              | CUSIP: 443201108<br>LEI: 549300HO5WFZUT5N2T22 | Long             | EC               | CORP              | US        |   1362822 | NS      | $267426561.06  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Hubspot Inc                                  | Hubspot Inc COM USD0.001                                              | CUSIP: 443573100<br>LEI: 529900BLNVEW0MVKJB37 | Long             | EC               | CORP              | US        |    137752 | NS      | $64440385.60   | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuit Inc                                   | Intuit Inc COM USD0.01                                                | CUSIP: 461202103<br>LEI: VI90HBPH7XSFMB9E4M29 | Long             | EC               | CORP              | US        |    514902 | NS      | $351631724.82  | 1.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                      | Intuitive Surgical Inc. COM USD0.001                                  | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |    353304 | NS      | $158008147.92  | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Johnson & Johnson                            | Johnson & Johnson COM USD1.00                                         | CUSIP: 478160104<br>LEI: 549300G0CFPGEF6X2043 | Long             | EC               | CORP              | US        |    533724 | NS      | $98963104.08   | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                    | KLA Corp. COM USD0.001                                                | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    199104 | NS      | $214753574.40  | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| LPL Financial Holdings Inc.                  | LPL Financial Holdings Inc. COM USD0.001                              | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |    313214 | NS      | $104203165.66  | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corporation                     | Lam Research Corporation COM NEW                                      | CUSIP: 512807306<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |   1811589 | NS      | $242571767.10  | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc                        | Lantheus Holdings Inc COM USD0.01                                     | CUSIP: 516544103<br>LEI: 5299005HUB5IOJGO7S39 | Long             | EC               | CORP              | US        |    400130 | NS      | $20522667.70   | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Lennar Corp.                                 | Lennar Corp. COM USD0.10 CLASS A                                      | CUSIP: 526057104<br>LEI: 529900G61XVRLX5TJX09 | Long             | EC               | CORP              | US        |    941811 | NS      | $118705858.44  | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del                       | Liberty Media Corp Del COM LBTY ONE S C                               | CUSIP: 531229755<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |   1734249 | NS      | $181142308.05  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                              | Eli Lilly & Co. COM NPV                                               | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |    769952 | NS      | $587473376.00  | 2.05%             |  |  |  | No            |                  1 | On Loan: No      |
| MasterCard Incorporated                      | MasterCard Incorporated COM USD0.0001 CLASS A                         | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |    964804 | NS      | $548790163.24  | 1.92%             |  |  |  | No            |                  1 | On Loan: No      |
| Mckesson Corp.                               | Mckesson Corp. COM USD0.01                                            | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |    172873 | NS      | $133551307.42  | 0.47%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp.                              | Microsoft Corp. COM USD0.00000625                                     | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |   6944010 | NS      | $3596649979.50 | 12.58%            |  |  |  | No            |                  1 | On Loan: No      |
| Monster Beverage Corp                        | Monster Beverage Corp COM USD0.005                                    | CUSIP: 61174X109<br>LEI: 52990090AP0E7HCB6F33 | Long             | EC               | CORP              | US        |   1442369 | NS      | $97085857.39   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlas Energy Solutions Inc.                  | Atlas Energy Solutions Inc. COM NEW                                   | CUSIP: 642045108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2460222 | NS      | $27972724.14   | 0.10%             |  |  |  | No            |                  1 | On Loan: —       |
| JANUS CAPITAL GROUP INC                      | Janus Henderson Cash Liquidity Fund LLC                               | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |  71451561 | NS      | $71465851.18   | 0.25%             |  |  |  | No            |                  2 | On Loan: No      |
| Eaton Corporation PLC                        | Eaton Corporation PLC COM USD0.01                                     | CUSIP: G29183103<br>LEI: 549300VDIGTMXUNT7H71 | Long             | EC               | CORP              | US        |    496746 | NS      | $185907190.50  | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Flutter Entertainment PLC                    | Flutter Entertainment PLC ORD EUR0.09 (DI)                            | CUSIP: G3643J108<br>LEI: 635400EG4YIJLJMZJ782 | Long             | EC               | CORP              | US        |    540703 | NS      | $137338562.00  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Netflix, Inc.                                | Netflix, Inc. COM USD0.001                                            | CUSIP: 64110L106<br>LEI: 549300Y7VHGU0I7CE873 | Long             | EC               | CORP              | US        |    491777 | NS      | $589601280.84  | 2.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Nike, Inc.                                   | Nike, Inc. CLASS'B'COM NPV                                            | CUSIP: 654106103<br>LEI: 787RXPR0UX0O0XUXPZ81 | Long             | EC               | CORP              | US        |    927855 | NS      | $64699329.15   | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| NVIDIA Corp.                                 | NVIDIA Corp. COM USD0.001                                             | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |  23263804 | NS      | $4340560550.32 | 15.18%            |  |  |  | No            |                  1 | On Loan: No      |
| O'Reilly Automotive Inc.                     | O'Reilly Automotive Inc. USD0.01                                      | CUSIP: 67103H107<br>LEI: 549300K2RLMQL149Q332 | Long             | EC               | CORP              | US        |   1504768 | NS      | $162229038.08  | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| ON Semiconductor Corp.                       | ON Semiconductor Corp. COM USD0.01                                    | CUSIP: 682189105<br>LEI: ZV20P4CNJVT8V1ZGJ064 | Long             | EC               | CORP              | US        |   1418329 | NS      | $69937802.99   | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Oracle Corp.                                 | Oracle Corp. COM USD0.01                                              | CUSIP: 68389X105<br>LEI: 1Z4GXXU7ZHVWFCD8TV52 | Long             | EC               | CORP              | US        |   1583821 | NS      | $445433818.04  | 1.56%             |  |  |  | No            |                  1 | On Loan: No      |
| The Procter & Gamble Co.                     | The Procter & Gamble Co. COM NPV                                      | CUSIP: 742718109<br>LEI: 2572IBTT8CCZW6AU4141 | Long             | EC               | CORP              | US        |   1564600 | NS      | $240400790.00  | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| The Progressive Corporation                  | The Progressive Corporation COM USD1.00                               | CUSIP: 743315103<br>LEI: 529900TACNVLY9DCR586 | Long             | EC               | CORP              | US        |    424738 | NS      | $104889049.10  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Servicenow Inc                               | Servicenow Inc COM USD0.001                                           | CUSIP: 81762P102<br>LEI: 549300HJTQM36M0E1G39 | Long             | EC               | CORP              | US        |    224135 | NS      | $206266957.80  | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Snowflake Inc                                | Snowflake Inc COM SHS                                                 | CUSIP: 833445109<br>LEI: 254900CJNSY1K7T53Z16 | Long             | EC               | CORP              | US        |    730683 | NS      | $164805550.65  | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Synopsys Inc                                 | Synopsys Inc COM USD0.01                                              | CUSIP: 871607107<br>LEI: QG7T915N9S0NY5UKNE63 | Long             | EC               | CORP              | US        |    135410 | NS      | $66809939.90   | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| T-Mobile US Inc                              | T-Mobile US Inc COM USD0.00001                                        | CUSIP: 872590104<br>LEI: 549300QHIJYOHPACPG31 | Long             | EC               | CORP              | US        |    404406 | NS      | $96806708.28   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Tyler Technologies Inc.                      | Tyler Technologies Inc. COM USD0.01                                   | CUSIP: 902252105<br>LEI: 25490040SMU4Z8LBCW70 | Long             | EC               | CORP              | US        |    190544 | NS      | $99684999.04   | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Trane Technologies PLC                       | Trane Technologies PLC COM USD1                                       | CUSIP: G8994E103<br>LEI: 549300BURLR9SLYY2705 | Long             | EC               | CORP              | US        |    504193 | NS      | $212749278.28  | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Spotify Technology S.A.                      | Spotify Technology S.A. COM EUR0.000625                               | CUSIP: L8681T102<br>LEI: 549300B4X0JHWV0DTD60 | Long             | EC               | CORP              | US        |    383834 | NS      | $267916132.00  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Royal Caribbean Group                        | Royal Caribbean Group COM USD0.01                                     | CUSIP: V7780T103<br>LEI: K2NEH8QNVW44JIWK7Z55 | Long             | EC               | CORP              | US        |    424502 | NS      | $137360357.16  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                          | Royal Bank of Canada                                                  | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |   4024901 | PA      | $4024901.00    | 0.01%             | 2025-10-01      | Fixed         | 4.31%                 | No            |                  2 | On Loan: No      |
| Janus Henderson Cash Collateral Fund         | Janus Henderson Cash Collateral Fund                                  | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |  16099603 | NS      | $16099603.00   | 0.06%             |  |  |  | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2025-11-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER