# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001145549-25-057303
**Filing Date:** 2025-8
**Character Count:** 55989
**Document Hash:** 91481c4096d07c47344d1f1fc66a3306
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001145549-25-057303.hdr.sgml**: 20250829

**ACCESSION NUMBER**: 0001145549-25-057303

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 2

**CONFORMED PERIOD OF REPORT**: 20250630

**FILED AS OF DATE**: 20250829

**DATE AS OF CHANGE**: 20250829

**PERIOD START**: 20250930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 251279164

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Global Technology and Innovation Fund (Series ID: S000010465)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028919 | Class T      | JAGTX           |
| C000077655 | Class A      | JATAX           |
| C000077656 | Class C      | JAGCX           |
| C000077657 | Class I      | JATIX           |
| C000077658 | Class S      | JATSX           |
| C000083513 | Class D      | JNGTX           |
| C000177052 | Class N      | JATNX           |

## Nport-Ex

**Janus Henderson Global Technology and Innovation Fund**

**Schedule of Investments (unaudited)**

**June 30, 2025**

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | |
|:---|:---|:---|
|  | *Shares or* <br>*Principal Amounts*<br>| *Value* |
| Common Stocks – 98.9% |  |  |
| Diversified Financial Services – 1.8% |  |  |
| &nbsp;&nbsp;&nbsp; Mastercard Inc - Class A | &nbsp;&nbsp;&nbsp;&nbsp; 215450 | &nbsp;&nbsp; $121069973 |
| &nbsp;&nbsp;&nbsp; Toast Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 551387 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 24420930 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 145490903 |
| Electronic Equipment, Instruments & Components – 1.4% |  |  |
| &nbsp;&nbsp;&nbsp; Amphenol Corp | &nbsp;&nbsp; 1155472 | &nbsp;&nbsp;&nbsp;&nbsp; 114102860 |
| Entertainment – 1.5% |  |  |
| &nbsp;&nbsp;&nbsp; Netflix Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 72422 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 96982473 |
| &nbsp;&nbsp;&nbsp; Spotify Technology SA<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 30044 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 23053963 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 120036436 |
| Hotels, Restaurants & Leisure – 2.7% |  |  |
| &nbsp;&nbsp;&nbsp; Booking Holdings Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 14190 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 82149315 |
| &nbsp;&nbsp;&nbsp; DoorDash Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 536566 | &nbsp;&nbsp;&nbsp;&nbsp; 132268885 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 214418200 |
| Independent Power and Renewable Electricity Producers – 0.8% |  |  |
| &nbsp;&nbsp;&nbsp; Vistra Corp | &nbsp;&nbsp;&nbsp;&nbsp; 318865 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 61799226 |
| Information Technology Services – 2.3% |  |  |
| &nbsp;&nbsp;&nbsp; Shopify Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 836213 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 96457169 |
| &nbsp;&nbsp;&nbsp; Snowflake Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 406641 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 90994057 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 187451226 |
| Interactive Media & Services – 3.4% |  |  |
| &nbsp;&nbsp;&nbsp; Alphabet Inc - Class C | &nbsp;&nbsp;&nbsp;&nbsp; 650084 | &nbsp;&nbsp;&nbsp;&nbsp; 115318401 |
| &nbsp;&nbsp;&nbsp; Meta Platforms Inc - Class A | &nbsp;&nbsp;&nbsp;&nbsp; 218328 | &nbsp;&nbsp;&nbsp;&nbsp; 161145713 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 276464114 |
| Multiline Retail – 6.8% |  |  |
| &nbsp;&nbsp;&nbsp; Amazon.com Inc<sup>\*</sup> <br>| &nbsp;&nbsp; 1506553 | &nbsp;&nbsp;&nbsp;&nbsp; 330522663 |
| &nbsp;&nbsp;&nbsp; MercadoLibre Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 81278 | &nbsp;&nbsp;&nbsp;&nbsp; 212430619 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 542953282 |
| Semiconductor & Semiconductor Equipment – 39.2% |  |  |
| &nbsp;&nbsp;&nbsp; Analog Devices Inc | &nbsp;&nbsp;&nbsp;&nbsp; 434777 | &nbsp;&nbsp;&nbsp;&nbsp; 103485621 |
| &nbsp;&nbsp;&nbsp; ASM International NV | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 46216 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 29579024 |
| &nbsp;&nbsp;&nbsp; ASML Holding NV | &nbsp;&nbsp;&nbsp;&nbsp; 248412 | &nbsp;&nbsp;&nbsp;&nbsp; 198252110 |
| &nbsp;&nbsp;&nbsp; Broadcom Inc | &nbsp;&nbsp; 1666991 | &nbsp;&nbsp;&nbsp;&nbsp; 459506069 |
| &nbsp;&nbsp;&nbsp; KLA Corp | &nbsp;&nbsp;&nbsp;&nbsp; 168956 | &nbsp;&nbsp;&nbsp;&nbsp; 151340647 |
| &nbsp;&nbsp;&nbsp; Lam Research Corp | &nbsp;&nbsp;&nbsp;&nbsp; 444553 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 43272789 |
| &nbsp;&nbsp;&nbsp; Nova Ltd<sup>\*,#</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 190509 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 52428077 |
| &nbsp;&nbsp;&nbsp; NVIDIA Corp | &nbsp;&nbsp; 7290102 | &nbsp;&nbsp; 1151763214 |
| &nbsp;&nbsp;&nbsp; NXP Semiconductors NV | &nbsp;&nbsp;&nbsp;&nbsp; 177359 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 38751168 |
| &nbsp;&nbsp;&nbsp; ON Semiconductor Corp<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 918486 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 48137851 |
| &nbsp;&nbsp;&nbsp; SK Hynix Inc | &nbsp;&nbsp;&nbsp;&nbsp; 501954 | &nbsp;&nbsp;&nbsp;&nbsp; 108627116 |
| &nbsp;&nbsp;&nbsp; Taiwan Semiconductor Manufacturing Co Ltd | 21010000 | &nbsp;&nbsp;&nbsp;&nbsp; 762508988 |
|  |  | &nbsp;&nbsp; 3147652674 |
| Software – 33.6% |  |  |
| &nbsp;&nbsp;&nbsp; ANSYS Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 105968 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 37218081 |
| &nbsp;&nbsp;&nbsp; Appfolio Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 96027 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 22113098 |
| &nbsp;&nbsp;&nbsp; Atlassian Corp - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 202865 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 41199853 |
| &nbsp;&nbsp;&nbsp; Autodesk Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 420203 | &nbsp;&nbsp;&nbsp;&nbsp; 130082243 |
| &nbsp;&nbsp;&nbsp; Cadence Design Systems Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 681249 | &nbsp;&nbsp;&nbsp;&nbsp; 209926879 |
| &nbsp;&nbsp;&nbsp; CCC Intelligent Solutions Holdings Inc<sup>\*</sup> <br>| &nbsp;&nbsp; 3654267 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 34386653 |
| &nbsp;&nbsp;&nbsp; Constellation Software Inc/Canada | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 52347 | &nbsp;&nbsp;&nbsp;&nbsp; 191964630 |
| &nbsp;&nbsp;&nbsp; Datadog Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 555612 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 74635360 |
| &nbsp;&nbsp;&nbsp; Dynatrace Inc<sup>\*</sup> <br>| &nbsp;&nbsp; 1213638 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 67004954 |
| &nbsp;&nbsp;&nbsp; HubSpot Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 99438 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 55350174 |
| &nbsp;&nbsp;&nbsp; Intuit Inc | &nbsp;&nbsp;&nbsp;&nbsp; 244377 | &nbsp;&nbsp;&nbsp;&nbsp; 192478657 |
| &nbsp;&nbsp;&nbsp; Kinaxis Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 208624 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 31030062 |
| &nbsp;&nbsp;&nbsp; Klaviyo Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 434091 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 14576776 |
| &nbsp;&nbsp;&nbsp; Lumine Group Inc<sup>\*</sup> <br>| &nbsp;&nbsp; 1110596 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 39012784 |
| &nbsp;&nbsp;&nbsp; Microsoft Corp | &nbsp;&nbsp; 2021596 | &nbsp;&nbsp; 1005562066 |
| &nbsp;&nbsp;&nbsp; Monday.com Ltd<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 106186 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 33393373 |
| &nbsp;&nbsp;&nbsp; Oracle Corp | &nbsp;&nbsp;&nbsp;&nbsp; 436302 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 95388706 |
| &nbsp;&nbsp;&nbsp; Procore Technologies Inc<sup>\*</sup> <br>| &nbsp;&nbsp; 1067379 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 73030071 |
| &nbsp;&nbsp;&nbsp; Samsara Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 683874 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 27204508 |
| &nbsp;&nbsp;&nbsp; ServiceNow Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 73925 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 76000814 |
| &nbsp;&nbsp;&nbsp; ServiceTitan Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 182001 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 19506867 |
| &nbsp;&nbsp;&nbsp; Synopsys Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 275771 | &nbsp;&nbsp;&nbsp;&nbsp; 141382276 |

---

------

---

| | | |
|:---|:---|:---|
|  | *Shares or*<br> *Principal Amounts*<br>| *Value* |
| Common Stocks – (continued) |  |  |
| Software – (continued) |  |  |
| &nbsp;&nbsp;&nbsp; Tyler Technologies Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 108025 | &nbsp;&nbsp;&nbsp;&nbsp; $64041541 |
| &nbsp;&nbsp;&nbsp; Workday Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 91958 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 22069920 |
|  |  | &nbsp;&nbsp; 2698560346 |
| Technology Hardware, Storage & Peripherals – 4.7% |  |  |
| &nbsp;&nbsp;&nbsp; Apple Inc | &nbsp;&nbsp; 1836114 | &nbsp;&nbsp;&nbsp;&nbsp; 376715509 |
| Wireless Telecommunication Services – 0.7% |  |  |
| &nbsp;&nbsp;&nbsp; T-Mobile US Inc | &nbsp;&nbsp;&nbsp;&nbsp; 235287 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 56059481 |
| Total Common Stocks (cost $4,155,479,733) |  | &nbsp;&nbsp; 7941704257 |
| Private Placements – 0.8% |  |  |
| Professional Services – 0.2% |  |  |
| &nbsp;&nbsp;&nbsp; Apartment List Inc<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp; 3783673 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 14983345 |
| Software – 0.6% |  |  |
| &nbsp;&nbsp;&nbsp; Magic Leap Inc - Class A private equity common shares<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 18847 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 0 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - private equity common shares<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 78474 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 5413921 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - Series A<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 50741 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 3500622 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - Series B<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 9272 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 639675 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - Series C<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 8331 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 574756 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - Series D<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 29804 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2056178 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - Series E<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 13836 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 954546 |
| &nbsp;&nbsp;&nbsp; Via Transportation Inc - Series G-1<sup>\*,</sup><sup>¢</sup><sup>,</sup><sup>§</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 563981 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 38909049 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 52048747 |
| Total Private Placements (cost $56,880,997) |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 67032092 |
| Warrants – 0% |  |  |
| Road & Rail – 0% |  |  |
| &nbsp;&nbsp;&nbsp; Grab Holdings Ltd, expires 12/1/26<sup>\*</sup> (cost $986,182) | &nbsp;&nbsp;&nbsp;&nbsp; 333275 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 130311 |
| Investment Companies – 0.3% |  |  |
| Money Markets – 0.3% |  |  |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº,</sup><sup>£</sup> (cost $25,753,685) | 25748536 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 25753685 |
| Investments Purchased with Cash Collateral from Securities Lending – 0% |  |  |
| Investment Companies – 0% |  |  |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº,</sup><sup>£</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 357084 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 357084 |
| Time Deposits – 0% |  |  |
| &nbsp;&nbsp;&nbsp; Royal Bank of Canada, 4.3100%, 7/1/25 | &nbsp;&nbsp;&nbsp;&nbsp; $89271 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 89271 |
| Total Investments Purchased with Cash Collateral from Securities Lending (cost $446,355) | Total Investments Purchased with Cash Collateral from Securities Lending (cost $446,355) | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 446355 |
| Total Investments (total cost $4,239,546,952) – 100.0% |  | &nbsp;&nbsp; 8035066700 |
| Liabilities, net of Cash, Receivables and Other Assets – (0)% |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (1942058) |
| Net Assets – 100% |  | $8033124642 |

---

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | |
|:---|:---|:---|
| ***Summary of Investments by Country - (Long Positions) (unaudited)*** |  |  |
| *Country* | *Value* | &nbsp;&nbsp; *% of* <br>*Investment* <br>*Securities*<br>|
| United States | $6256198474 | &nbsp;&nbsp;&nbsp;&nbsp; 77.9<br> %<br>|
| Taiwan | 762508988 | &nbsp;&nbsp;&nbsp;&nbsp;9.5 |
| Canada | 358464645 | &nbsp;&nbsp;&nbsp;&nbsp;4.5 |
| Netherlands | 227831134 | &nbsp;&nbsp;&nbsp;&nbsp;2.8 |
| Argentina | 212430619 | &nbsp;&nbsp;&nbsp;&nbsp;2.6 |
| South Korea | 108627116 | &nbsp;&nbsp;&nbsp;&nbsp;1.3 |
| Israel | 85821450 | &nbsp;&nbsp;&nbsp;&nbsp;1.1 |
| Sweden | 23053963 | &nbsp;&nbsp;&nbsp;&nbsp;0.3 |
| Singapore | 130311 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Total | $8035066700 | &nbsp;&nbsp;&nbsp;&nbsp; 100.0<br> %<br>|

---

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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|:---|
| ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** |
| *Affiliate* | *Affiliated* <br>*Investments,* <br>*at Value at* <br>*9/30/24*<br>| *Purchases* | *Sales* <br>*Proceeds*<br>| *Realized* <br>*Gain/(Loss)*<br>| *Change in* <br>*Unrealized* <br>*Appreciation/* <br>*Depreciation*<br>| *Affiliated* <br>*Investments,* <br>*at Value at* <br>*6/30/25*<br>| *Ending* <br>*Shares*<br>| *Dividend* <br>*Income*<br>|
| Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% | Investment Companies - 0.3% |
| Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% | Money Markets - 0.3% |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  |
|  | &nbsp;&nbsp; $354177309 | &nbsp;&nbsp; $1243105347 | &nbsp;&nbsp; $(1571528971) | &nbsp;&nbsp; $(10375) | &nbsp;&nbsp; $10375 | &nbsp;&nbsp; $25753685 | &nbsp;&nbsp; 25748536 | &nbsp;&nbsp; $6332007 |
| Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% | Investments Purchased with Cash Collateral from Securities Lending - 0% |
| Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% | Investment Companies - 0% |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Collateral Fund LLC, 4.2933%<sup>ºº</sup>  |
|  | &nbsp;&nbsp; 1285451 | &nbsp;&nbsp; 80989717 | &nbsp;&nbsp; (81918084) | &nbsp;&nbsp; - | &nbsp;&nbsp; - | &nbsp;&nbsp; 357084 | &nbsp;&nbsp; 357084 | &nbsp;&nbsp; 89537 <br><sup>∆</sup><br>|
| Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% | Total Affiliated Investments - 0.3% |
|  | &nbsp;&nbsp; $355462760 | &nbsp;&nbsp; $1324095064 | &nbsp;&nbsp; $(1653447055) | &nbsp;&nbsp; $(10375) | &nbsp;&nbsp; $10375 | &nbsp;&nbsp; $26110769 | &nbsp;&nbsp; 26105620 | &nbsp;&nbsp; $6421544 |

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**Notes to Schedule of Investments (unaudited)** 

LLC Limited Liability Company

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

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| |
|:---|
| Non-income producing security. |
| Rate shown is the 7-day yield as of June 30, 2025. |
| Loaned security; a portion of the security is on loan at June 30, 2025. |
| &nbsp;&nbsp; Security is valued using significant unobservable inputs. The total value of Level 3 securities as of the period ended June 30, 2025 is <br> $67,032,092, which represents 0.8% of net assets.<br>|
| £&nbsp;&nbsp; The Fund may invest in certain securities that are considered affiliated companies. As defined by the Investment Company Act of 1940, as <br> amended, an affiliated company is one in which the Fund owns 5% or more of the outstanding voting securities, or a company which is <br> under common ownership or control.<br>|
| Net of income paid to the securities lending agent and rebates paid to the borrowing counterparties. |
| Schedule of Restricted Securities (as of June 30, 2025) |

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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

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| | | | | |
|:---|:---|:---|:---|:---|
|  | *Acquisition* <br>*Date*<br>| *Cost* | *Value* | &nbsp;&nbsp; *Value as a* <br>*% of Net* <br>*Assets*<br>|
| Apartment List Inc | 11/2/20 | &nbsp;&nbsp;&nbsp; $13821757 | $14983345 | &nbsp;&nbsp;&nbsp;&nbsp; 0.2<br> %<br>|
| Magic Leap Inc - Class A private equity common shares | 10/5/17 | &nbsp;&nbsp;&nbsp; 9160263 | 0 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Via Transportation Inc - private equity common shares | 12/2/21 | &nbsp;&nbsp;&nbsp; 3392431 | 5413921 | &nbsp;&nbsp;&nbsp;&nbsp;0.1 |
| Via Transportation Inc - Series A | 12/2/21 | &nbsp;&nbsp;&nbsp; 2193533 | 3500622 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Via Transportation Inc - Series B | 12/2/21 | &nbsp;&nbsp;&nbsp; 400829 | 639675 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Via Transportation Inc - Series C | 12/2/21 | &nbsp;&nbsp;&nbsp; 360149 | 574756 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Via Transportation Inc - Series D | 12/2/21 | &nbsp;&nbsp;&nbsp; 1288427 | 2056178 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Via Transportation Inc - Series E | 12/2/21 | &nbsp;&nbsp;&nbsp; 598130 | 954546 | &nbsp;&nbsp;&nbsp;&nbsp;0.0 |
| Via Transportation Inc - Series G-1 | 11/4/21-2/2/23 | &nbsp;&nbsp;&nbsp; 25665478 | 38909049 | &nbsp;&nbsp;&nbsp;&nbsp;0.5 |
| Total |  | &nbsp;&nbsp;&nbsp; $56880997 | $67032092 | &nbsp;&nbsp;&nbsp; 0.8% |

---

The Fund has registration rights for certain restricted securities held as of June 30, 2025. The issuer incurs all registration costs.

The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial

instruments as of June 30, 2025.

---

| | | | |
|:---|:---|:---|:---|
| **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** |
|  | ***Level 1 -*** <br>***Quoted Prices***<br>| &nbsp;&nbsp; ***Level 2 -*** <br>***Other Significant*** <br>***Observable Inputs***<br>| &nbsp;&nbsp; ***Level 3 -*** <br>***Significant*** <br>***Unobservable Inputs***<br>|
| **Assets** |  |  |  |
| **Investments In Securities:** |  |  |  |
| *Common Stocks* | $7941704257 | &nbsp;&nbsp;&nbsp; $- | &nbsp;&nbsp;&nbsp; $- |
| *Private Placements* | - | &nbsp;&nbsp;&nbsp; - | &nbsp;&nbsp;&nbsp; 67032092 |
| *Warrants* | 130311 | &nbsp;&nbsp;&nbsp; - | &nbsp;&nbsp;&nbsp; - |
| *Investment Companies* | - | &nbsp;&nbsp;&nbsp; 25753685 | &nbsp;&nbsp;&nbsp; - |
| *Investments Purchased with Cash Collateral from Securities Lending* | - | &nbsp;&nbsp;&nbsp; 446355 | &nbsp;&nbsp;&nbsp; - |
| **Total Assets** | $7941834568 | &nbsp;&nbsp;&nbsp; $26200040 | &nbsp;&nbsp;&nbsp; $67032092 |

---

------

**Investment Valuation**

Fund holdings are valued in accordance with policies and procedures established by the Adviser pursuant to Rule 2a-5 under the 1940 Act and approved by and subject to the oversight of the Trustees (the "Valuation Procedures"). Equity securities traded on a domestic securities exchange are generally valued at readily available market quotations, which are (i) the official close prices or (ii) last sale prices on the primary market or exchange in which the securities trade. If such price is lacking for the trading period immediately preceding the time of determination, such securities are generally valued at their current bid price. Equity securities that are traded on a foreign exchange are generally valued at the closing prices on such markets. In the event that there is no current trading volume on a particular security in such foreign exchange, the bid price from the primary exchange is generally used to value the security. Foreign securities and currencies are converted to U.S. dollars using the current spot USD dollar exchange rate in effect at the close of the New York Stock Exchange ("NYSE"). The Adviser will determine the market value of individual securities held by it by using prices provided by one or more Adviser-approved professional pricing services or, as needed, by obtaining market quotations from independent broker-dealers. Most debt securities are valued in accordance with the evaluated bid price supplied by the pricing service that is intended to reflect market value. The evaluated bid price supplied by the pricing service is an evaluation that may consider factors such as security prices, yields, maturities, and ratings. Certain short-term securities maturing within 60 days or less may be evaluated and valued on an amortized cost basis provided that the amortized cost determined approximates market value. Securities for which market quotations or evaluated prices are not readily available or deemed unreliable are valued at fair value determined in good faith by the Adviser pursuant to the Valuation Procedures. Circumstances in which fair valuation may be utilized include, but are not limited to: (i) a significant event that may affect the securities of a single issuer, such as a merger, bankruptcy, or significant issuer-specific development; (ii) an event that may affect an entire market, such as a natural disaster or significant governmental action; (iii) a nonsignificant event such as a market closing early or not opening, or a security trading halt; and (iv) pricing of a non-valued security and a restricted or nonpublic security. Special valuation considerations may apply with respect to "odd-lot" fixed-income transactions which, due to their small size, may receive evaluated prices by pricing services which reflect a large block trade and not what actually could be obtained for the odd-lot position. The value of the securities of other mutual funds held by the Fund, if any, will be calculated using the NAV of such mutual funds, and the prospectuses for such mutual funds explain the circumstances under which they use fair valuation and the effects of using fair valuation. The value of the securities of any cash management pooled investment vehicles that

operate as money market funds held by the Fund, if any, will be calculated using the NAV of such funds.

**Valuation Inputs Summary**

FASB ASC 820, *Fair Value Measurements and Disclosures* ("ASC 820"), defines fair value, establishes a framework for measuring fair value, and expands disclosure requirements regarding fair value measurements. This standard emphasizes that fair value is a market-based measurement that should be determined based on the assumptions that market participants would use in pricing an asset or liability and establishes a hierarchy that prioritizes inputs to valuation techniques used to measure fair value. These inputs are summarized into three broad levels:

Level 1 – Unadjusted quoted prices in active markets the Fund has the ability to access for identical assets or liabilities.

Level 2 – Observable inputs other than unadjusted quoted prices included in Level 1 that are observable for the asset or liability either directly or indirectly. These inputs may include quoted prices for the identical instrument on an inactive market, prices for similar instruments, interest rates, prepayment speeds, credit risk, yield curves, default

rates and similar data.

Assets or liabilities categorized as Level 2 in the hierarchy generally include: debt securities fair valued in accordance with the evaluated bid or ask prices supplied by a pricing service; securities traded on OTC markets and listed securities for which no sales are reported that are fair valued at the latest bid price (or yield equivalent thereof) obtained from one or more dealers transacting in a market for such securities or by a pricing service approved by the Fund's Trustees; certain short-term debt securities with maturities of 60 days or less that are fair valued at amortized cost; and equity securities of foreign issuers whose fair value is determined by using systematic fair valuation models provided by independent third parties in order to adjust for stale pricing which may occur between the close of certain foreign exchanges and the close of the NYSE. Other securities that may be categorized as Level 2 in the hierarchy include, but are not limited to, preferred stocks, bank loans, swaps,

investments in unregistered investment companies, options, and forward contracts.

Level 3 – Unobservable inputs for the asset or liability to the extent that relevant observable inputs are not available, representing the Fund's own assumptions about the assumptions that a market participant would use in valuing the asset or liability, and that would be based on the best information available.

There have been no significant changes in valuation techniques used in valuing any such positions held by the Fund since the beginning of the fiscal period.

------

The inputs or methodology used for fair valuing securities are not necessarily an indication of the risk associated with investing in those securities. The summary of inputs used as of June 30, 2025 to fair value the Fund's investments in securities and other financial instruments is included in the "Valuation Inputs Summary" in the Notes to Schedule of

Investments.

The Fund's management has determined the Fund did not hold a significant amount of Level 3 securities as of June 30, 2025.

------

For additional information on the Fund, please refer to the Fund's most recent semiannual or annual financial

statements.

125-35-70237 08-25

------

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Global Technology and Innovation Fund

- **b. EDGAR series identifier (if any):** S000010465

- **c. LEI of Series:** 549300LSB3S6GZCG4692

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-09-30

- **b. Date as of which information is reported:** 2025-06-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $8039072393.68

**Total Liabilities:** $5947751.57

**Net Assets:** $8033124642.11

**Cash Not Reported:** $1070022.18

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028919 | 3.85%                | 11.29%               | 9.29%                |
| Class ID C000077655 | 3.84%                | 11.28%               | 9.29%                |
| Class ID C000077656 | 3.79%                | 11.22%               | 9.23%                |
| Class ID C000077657 | 3.87%                | 11.30%               | 9.30%                |
| Class ID C000077658 | 3.83%                | 11.26%               | 9.28%                |
| Class ID C000083513 | 3.87%                | 11.29%               | 9.31%                |
| Class ID C000177052 | 3.86%                | 11.32%               | 9.31%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $84300024.00             | $163525973.00                              |
| Month 2  | $10041693.00             | $738525728.00                              |
| Month 3  | $126029307.00            | $557254578.00                              |

**Designated Index Information**

- **Index Name:** ACWI/INFORMATION TECH (MSCI ACWI Information Technology Index)

- **Index Identifier:** MSCI.MC.106752

### Schedule of Portfolio Investments

| Name                                         | Title                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)    | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Alphabet Inc.                                | Alphabet Inc. CAP USD0.001 CL C                       | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |    650084 | NS      | $115318400.76  | 1.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Amazon.com, Inc.                             | Amazon.com, Inc. COM USD0.01                          | CUSIP: 023135106<br>LEI: ZXTILKJKG63JELOEG630 | Long             | EC               | CORP              | US        |   1506553 | NS      | $330522662.67  | 4.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Amphenol Corporation                         | Amphenol Corporation CL'A'COM USD0.001                | CUSIP: 032095101<br>LEI: 549300D3L3G0R4U4VT04 | Long             | EC               | CORP              | US        |   1155472 | NS      | $114102860.00  | 1.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Analog Devices Inc.                          | Analog Devices Inc. COM USD0.16 2/3                   | CUSIP: 032654105<br>LEI: GYVOE5EZ4GDAVTU4CQ61 | Long             | EC               | CORP              | US        |    434777 | NS      | $103485621.54  | 1.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Ansys Inc                                    | Ansys Inc COM USD0.01                                 | CUSIP: 03662Q105<br>LEI: 549300VJV8H15Z5FJ571 | Long             | EC               | CORP              | US        |    105968 | NS      | $37218080.96   | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                                   | Apple Inc. COM USD0.00001                             | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |   1836114 | NS      | $376715509.38  | 4.69%             |  |  |  | No            |                  1 | On Loan: No      |
| AppFolio Inc.                                | AppFolio Inc. COM USD0.0001 CL A                      | CUSIP: 03783C100<br>LEI: 529900OOUUGT6JFC0S15 | Long             | EC               | CORP              | US        |     96027 | NS      | $22113097.56   | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlassian Corporation                        | Atlassian Corporation CL A                            | CUSIP: 049468101<br>LEI: 549300V7ZY5P02D2MY38 | Long             | EC               | CORP              | US        |    202865 | NS      | $41199852.85   | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Autodesk Inc                                 | Autodesk Inc COM USD0.01                              | CUSIP: 052769106<br>LEI: FRKKVKAIQEF3FCSTPG55 | Long             | EC               | CORP              | US        |    420203 | NS      | $130082242.71  | 1.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                         | Booking Holdings Inc COM USD0.008                     | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |     14190 | NS      | $82149315.60   | 1.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadcom Inc                                 | Broadcom Inc COM USD0.001                             | CUSIP: 11135F101<br>LEI: 549300WV6GIDOZJTV909 | Long             | EC               | CORP              | US        |   1666991 | NS      | $459506069.15  | 5.72%             |  |  |  | No            |                  1 | On Loan: No      |
| CCC Intelligent Solutions Holdings Inc       | CCC Intelligent Solutions Holdings Inc COM            | CUSIP: 12510Q100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   3654267 | NS      | $34386652.47   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Cadence Design Systems, Inc.                 | Cadence Design Systems, Inc. COM USD0.01              | CUSIP: 127387108<br>LEI: GCT7RXJOGLXPV0NXZY22 | Long             | EC               | CORP              | US        |    681249 | NS      | $209926879.35  | 2.61%             |  |  |  | No            |                  1 | On Loan: No      |
| ASM International N.V.                       | ASM International N.V. EUR0.04                        | CUSIP: N07045201<br>LEI: 7245001I22ND6ZFHX623 | Long             | EC               | CORP              | NL        |     46216 | NS      | $29579023.84   | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Software Inc.                  | Constellation Software Inc. COM STK NPV               | CUSIP: 21037X100<br>LEI: 549300B6PYHMCTDWQV29 | Long             | EC               | CORP              | CA        |     52347 | NS      | $191964630.14  | 2.39%             |  |  |  | No            |                  1 | On Loan: No      |
| ASML Holding N.V.                            | ASML Holding N.V. EUR0.09                             | CUSIP: N07059202<br>LEI: 724500Y6DUVHQD6OXN27 | Long             | EC               | CORP              | NL        |    248412 | NS      | $198252109.68  | 2.47%             |  |  |  | No            |                  1 | On Loan: No      |
| SK Hynix Inc.                                | SK Hynix Inc. KRW5000                                 | CUSIP: Y8085F100<br>LEI: 988400XAIK6XISWQV045 | Long             | EC               | CORP              | KR        |    501954 | NS      | $108627116.28  | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Oracle Corp.                                 | Oracle Corp. COM USD0.01                              | CUSIP: 68389X105<br>LEI: 1Z4GXXU7ZHVWFCD8TV52 | Long             | EC               | CORP              | US        |    436302 | NS      | $95388706.26   | 1.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Procore Technologies Inc                     | Procore Technologies Inc COM                          | CUSIP: 74275K108<br>LEI: 5493005PTWY0RHYRAH22 | Long             | EC               | CORP              | US        |   1067379 | NS      | $73030071.18   | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Servicenow Inc                               | Servicenow Inc COM USD0.001                           | CUSIP: 81762P102<br>LEI: 549300HJTQM36M0E1G39 | Long             | EC               | CORP              | US        |     73925 | NS      | $76000814.00   | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - private equity common shares | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |     78474 | NS      | $5413921.26    | 0.07%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Series E                     | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |     13836 | NS      | $954545.64     | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Series G-1                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |     17973 | NS      | $1239957.27    | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Series D                     | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |     29804 | NS      | $2056177.96    | 0.03%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Series C                     | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |      8331 | NS      | $574755.69     | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Series A                     | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |     50741 | NS      | $3500621.59    | 0.04%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Preferred shares             | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    546008 | NS      | $37669091.92   | 0.47%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Via Transportation Inc                       | Via Transportation Inc - Series B                     | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |      9272 | NS      | $639675.28     | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| ON Semiconductor Corp.                       | ON Semiconductor Corp. COM USD0.01                    | CUSIP: 682189105<br>LEI: ZV20P4CNJVT8V1ZGJ064 | Long             | EC               | CORP              | US        |    918486 | NS      | $48137851.26   | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Co., Ltd. | Taiwan Semiconductor Manufacturing Co., Ltd. TWD10    | CUSIP: Y84629107<br>LEI: 549300KB6NK5SBD14S87 | Long             | EC               | CORP              | TW        |  21010000 | NS      | $762508987.57  | 9.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Samsara Inc                                  | Samsara Inc COM CL A                                  | CUSIP: 79589L106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    683874 | NS      | $27204507.72   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| ServiceTitan Inc.                            | ServiceTitan Inc. SHS CL A                            | CUSIP: 81764X103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    182001 | NS      | $19506867.18   | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                          | GRABW 2026-12-01 CALL 11.5                            | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               | CORP              | US        |    333275 | NS      | $130310.53     | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Shopify Inc                                  | Shopify Inc COM NPV CL A                              | CUSIP: 82509L107<br>LEI: 549300HGQ43STJLLP808 | Long             | EC               | CORP              | US        |    836213 | NS      | $96457169.55   | 1.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Snowflake Inc                                | Snowflake Inc CL A                                    | CUSIP: 833445109<br>LEI: 254900CJNSY1K7T53Z16 | Long             | EC               | CORP              | US        |    406641 | NS      | $90994056.57   | 1.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Synopsys Inc                                 | Synopsys Inc COM USD0.01                              | CUSIP: 871607107<br>LEI: QG7T915N9S0NY5UKNE63 | Long             | EC               | CORP              | US        |    275771 | NS      | $141382276.28  | 1.76%             |  |  |  | No            |                  1 | On Loan: No      |
| T-Mobile US Inc                              | T-Mobile US Inc COM USD0.00001                        | CUSIP: 872590104<br>LEI: 549300QHIJYOHPACPG31 | Long             | EC               | CORP              | US        |    235287 | NS      | $56059480.62   | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Toast Inc                                    | Toast Inc CL A                                        | CUSIP: 888787108<br>LEI: 549300TN1PZNLCD4I551 | Long             | EC               | CORP              | US        |    551387 | NS      | $24420930.23   | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Tyler Technologies Inc.                      | Tyler Technologies Inc. COM USD0.01                   | CUSIP: 902252105<br>LEI: 25490040SMU4Z8LBCW70 | Long             | EC               | CORP              | US        |    108025 | NS      | $64041541.00   | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Vistra Corp                                  | Vistra Corp COM USD0.01                               | CUSIP: 92840M102<br>LEI: 549300KP43CPCUJOOG15 | Long             | EC               | CORP              | US        |    318865 | NS      | $61799225.65   | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Workday Inc                                  | Workday Inc COM USD0.001 CL A                         | CUSIP: 98138H101<br>LEI: 549300J0DYC0N31V7G13 | Long             | EC               | CORP              | US        |     91958 | NS      | $22069920.00   | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Spotify Technology S.A.                      | Spotify Technology S.A. COM EUR0.000625               | CUSIP: L8681T102<br>LEI: 549300B4X0JHWV0DTD60 | Long             | EC               | CORP              | US        |     30044 | NS      | $23053962.96   | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Nova Ltd                                     | Nova Ltd ILS0.01                                      | CUSIP: M7516K103<br>LEI: 529900B2DSWE5V3SC292 | Long             | EC               | CORP              | US        |    190509 | NS      | $52428076.80   | 0.65%             |  |  |  | No            |                  1 | On Loan: —       |
| Magic Leap Inc                               | Magic Leap Inc - Class A private equity common shares | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |     18847 | NS      | $0.02          | 0.00%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Datadog Inc                                  | Datadog Inc COM USD0.00001 CL A                       | CUSIP: 23804L103<br>LEI: 549300F6JNO0KRPO1K63 | Long             | EC               | CORP              | US        |    555612 | NS      | $74635359.96   | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                                 | DoorDash Inc CL A                                     | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |    536566 | NS      | $132268884.66  | 1.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Dynatrace Inc                                | Dynatrace Inc COM USD0.001                            | CUSIP: 268150109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1213638 | NS      | $67004953.98   | 0.83%             |  |  |  | No            |                  1 | On Loan: No      |
| Meta Platforms Inc                           | Meta Platforms Inc COM USD0.000006 CL 'A'             | CUSIP: 30303M102<br>LEI: BQ4BKCS1HXDV9HN80Z93 | Long             | EC               | CORP              | US        |    218328 | NS      | $161145713.52  | 2.01%             |  |  |  | No            |                  1 | On Loan: No      |
| CRIMSON CAPITAL                              | Apartment List Inc                                    | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   3783673 | NS      | $14983345.08   | 0.19%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Hubspot Inc                                  | Hubspot Inc COM USD0.001                              | CUSIP: 443573100<br>LEI: 529900BLNVEW0MVKJB37 | Long             | EC               | CORP              | US        |     99438 | NS      | $55350173.94   | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuit Inc                                   | Intuit Inc COM USD0.01                                | CUSIP: 461202103<br>LEI: VI90HBPH7XSFMB9E4M29 | Long             | EC               | CORP              | US        |    244377 | NS      | $192478656.51  | 2.40%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                    | KLA Corp. COM USD0.001                                | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    168956 | NS      | $151340647.44  | 1.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Klaviyo Inc                                  | Klaviyo Inc COM SER A                                 | CUSIP: 49845K101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    434091 | NS      | $14576775.78   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corporation                     | Lam Research Corporation COM NEW                      | CUSIP: 512807306<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |    444553 | NS      | $43272789.02   | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| MasterCard Incorporated                      | MasterCard Incorporated COM USD0.0001 CLASS A         | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |    215450 | NS      | $121069973.00  | 1.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Mercadolibre Inc                             | Mercadolibre Inc COM USD0.001                         | CUSIP: 58733R102<br>LEI: 549300DKPDN9M5S8GB14 | Long             | EC               | CORP              | US        |     81278 | NS      | $212430619.14  | 2.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp.                              | Microsoft Corp. COM USD0.00000625                     | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |   2021596 | NS      | $1005562066.36 | 12.52%            |  |  |  | No            |                  1 | On Loan: No      |
| Netflix, Inc.                                | Netflix, Inc. COM USD0.001                            | CUSIP: 64110L106<br>LEI: 549300Y7VHGU0I7CE873 | Long             | EC               | CORP              | US        |     72422 | NS      | $96982472.86   | 1.21%             |  |  |  | No            |                  1 | On Loan: No      |
| NVIDIA Corp.                                 | NVIDIA Corp. COM USD0.001                             | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |   7290102 | NS      | $1151763214.98 | 14.34%            |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC                      | Janus Henderson Cash Liquidity Fund LLC               | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |  25748536 | NS      | $25753685.31   | 0.32%             |  |  |  | No            |                  2 | On Loan: No      |
| Kinaxis Inc                                  | Kinaxis Inc COM NPV                                   | CUSIP: 49448Q109<br>LEI: 5299009VAZ14K7PFUQ15 | Long             | EC               | CORP              | CA        |    208624 | NS      | $31030062.04   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Lumine Group Inc                             | Lumine Group Inc SUB VTG SHS                          | CUSIP: 55027C106<br>LEI: 984500E3C44B9D44M738 | Long             | EC               | CORP              | CA        |   1110596 | NS      | $39012783.99   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Monday.Com Limited                           | Monday.Com Limited SHS                                | CUSIP: M7S64H106<br>LEI: 984500MDAFVD86E3B328 | Long             | EC               | CORP              | US        |    106186 | NS      | $33393373.28   | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| NXP Semiconductors NV                        | NXP Semiconductors NV EUR0.20                         | CUSIP: N6596X109<br>LEI: 724500M9BY5293JDF951 | Long             | EC               | CORP              | US        |    177359 | NS      | $38751167.91   | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Janus Henderson Cash Collateral Fund         | Janus Henderson Cash Collateral Fund                  | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |    357084 | NS      | $357084.00     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                          | Royal Bank of Canada                                  | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |     89271 | PA      | $89271.00      | 0.00%             | 2025-07-01      | Fixed         | 4.31%                 | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2025-08-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER