# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0000277751-26-000135
**Filing Date:** 2026-5
**Character Count:** 46078
**Document Hash:** f0bd8e7b12b559724f17708ee2614e54
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0000277751-26-000135.hdr.sgml**: 20260529

**ACCESSION NUMBER**: 0000277751-26-000135

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20260331

**FILED AS OF DATE**: 20260529

**DATE AS OF CHANGE**: 20260529

**PERIOD START**: 20260930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 261042054

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Enterprise Fund (Series ID: S000010490)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028952 | Class T      | JAENX           |
| C000077737 | Class A      | JDMAX           |
| C000077738 | Class C      | JGRCX           |
| C000077739 | Class I      | JMGRX           |
| C000077740 | Class R      | JDMRX           |
| C000077741 | Class S      | JGRTX           |
| C000083535 | Class D      | JANEX           |
| C000114883 | Class N      | JDMNX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Enterprise Fund

- **b. EDGAR series identifier (if any):** S000010490

- **c. LEI of Series:** TJ2Y3QQ1QRI8KQ84FR29

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2026-09-30

- **b. Date as of which information is reported:** 2026-03-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $20440001531.74

**Total Liabilities:** $2026197.08

**Net Assets:** $20437975334.66

**Cash Not Reported:** $7958787.29

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028952 | -0.11%               | -0.07%               | -5.82%               |
| Class ID C000077737 | -0.14%               | -0.09%               | -5.83%               |
| Class ID C000077738 | -0.17%               | -0.14%               | -5.88%               |
| Class ID C000077739 | -0.10%               | -0.06%               | -5.81%               |
| Class ID C000077740 | -0.16%               | -0.12%               | -5.85%               |
| Class ID C000077741 | -0.14%               | -0.09%               | -5.83%               |
| Class ID C000083535 | -0.10%               | -0.06%               | -5.81%               |
| Class ID C000114883 | -0.10%               | -0.06%               | -5.80%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $275725357.00            | $-283562317.00                             |
| Month 2  | $436911024.00            | $-456294724.00                             |
| Month 3  | $304952814.00            | $-1612465351.00                            |

**Designated Index Information**

- **Index Name:** Russell MidCap Growth

- **Index Identifier:** RUSSELL.MC.RDG

### Schedule of Portfolio Investments

| Name                                        | Title                                                          | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Cboe Global Markets Inc                     | Cboe Global Markets Inc COM USD0.01                            | CUSIP: 12503M108<br>LEI: 529900RLNSGA90UPEH54 | Long             | EC               | CORP              | US        |    638291 | NS      | $179404451.37 | 0.88%             |  |  |  | No            |                  1 | On Loan: No      |
| CDW Corp                                    | CDW Corp COM USD0.01                                           | CUSIP: 12514G108<br>LEI: 9845001B052ABF0B6755 | Long             | EC               | CORP              | US        |   1411060 | NS      | $170766481.20 | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Canadian Pacific Kansas City Ltd.           | Canadian Pacific Kansas City Ltd. COM                          | CUSIP: 13646K108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   3784687 | NS      | $297703479.42 | 1.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Carmax Inc                                  | Carmax Inc COM USD0.50                                         | CUSIP: 143130102<br>LEI: 529900F60CID3NCZM330 | Long             | EC               | CORP              | US        |   3156983 | NS      | $131267353.14 | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Clean Harbors, Inc.                         | Clean Harbors, Inc. COM USD0.01                                | CUSIP: 184496107<br>LEI: 5493000SJVZVZJKHJF48 | Long             | EC               | CORP              | US        |   1443210 | NS      | $413811603.30 | 2.02%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cooper Companies, Inc.                  | The Cooper Companies, Inc. COM                                 | CUSIP: 216648501<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |   1678964 | NS      | $120045926.00 | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Corteva Inc                                 | Corteva Inc COM USD0.01                                        | CUSIP: 22052L104<br>LEI: 549300WZN9I2QKLS0O94 | Long             | EC               | CORP              | US        |   4946203 | NS      | $414046653.13 | 2.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Costar Group Inc                            | Costar Group Inc COM USD0.01                                   | CUSIP: 22160N109<br>LEI: 984500Q1D53D08E2E698 | Long             | EC               | CORP              | US        |   5532268 | NS      | $223171691.12 | 1.09%             |  |  |  | No            |                  1 | On Loan: No      |
| DTE Energy Co.                              | DTE Energy Co. COM NPV                                         | CUSIP: 233331107<br>LEI: 549300IX8SD6XXD71I78 | Long             | EC               | CORP              | US        |   2204308 | NS      | $322313915.76 | 1.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Descartes Systems Group Inc.                | Descartes Systems Group Inc. COM NPV                           | CUSIP: 249906108<br>LEI: 875500NEPA7JI5EOMN45 | Long             | EC               | CORP              | US        |   1369078 | NS      | $97971221.68  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Dollar Tree Inc                             | Dollar Tree Inc COM USD0.01                                    | CUSIP: 256746108<br>LEI: 549300PMSTQITB1WHR43 | Long             | EC               | CORP              | US        |    904605 | NS      | $99063293.55  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                                | DoorDash Inc CL A                                              | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |   2748340 | NS      | $412663251.00 | 2.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Dynatrace Inc                               | Dynatrace Inc COM USD0.001                                     | CUSIP: 268150109<br>LEI: 6488T0Z33CE9L61EX904 | Long             | EC               | CORP              | US        |   2457390 | NS      | $90874282.20  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson Enterprises Inc                    | Ferguson Enterprises Inc COMMON STOCK NEW                      | CUSIP: 31488V107<br>LEI: 2138003JYQMRP3SLX189 | Long             | EC               | CORP              | US        |   3174027 | NS      | $740373538.02 | 3.62%             |  |  |  | No            |                  1 | On Loan: No      |
| FirstService Corp.                          | FirstService Corp. COM NPV                                     | CUSIP: 33767E202<br>LEI: 5493000XUDIV75BCF118 | Long             | EC               | CORP              | US        |   1027776 | NS      | $142799197.44 | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Gildan Activewear Inc.                      | Gildan Activewear Inc. COM NPV                                 | CUSIP: 375916103<br>LEI: 549300SB3IBI34M00Z45 | Long             | EC               | CORP              | US        |   5492104 | NS      | $305635587.60 | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Glaukos Corp.                               | Glaukos Corp. COM USD0.001                                     | CUSIP: 377322102<br>LEI: 5299004JA1LHE21M6T14 | Long             | EC               | CORP              | US        |    636302 | NS      | $68504273.32  | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Globus Med Inc                              | Globus Med Inc COM USD0.001 CL 'A'                             | CUSIP: 379577208<br>LEI: 5299003M2P4CIK0RF205 | Long             | EC               | CORP              | US        |   2207141 | NS      | $190167268.56 | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| The Charles Schwab Corporation              | The Charles Schwab Corporation COM USD0.01                     | CUSIP: 808513105<br>LEI: 549300VSGCJ7E698NM85 | Long             | EC               | CORP              | US        |   1364178 | NS      | $128205448.44 | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Software Inc.                 | Constellation Software Inc. COM STK NPV                        | CUSIP: 21037X100<br>LEI: 549300B6PYHMCTDWQV29 | Long             | EC               | CORP              | CA        |    232661 | NS      | $408480721.40 | 2.00%             |  |  |  | No            |                  2 | On Loan: No      |
| Entain PLC                                  | Entain PLC ORD EUR0.01                                         | CUSIP: G3167C109<br>LEI: 213800GNI3K45LQR8L28 | Long             | EC               | CORP              | GB        |  11134775 | NS      | $84067864.97  | 0.41%             |  |  |  | No            |                  2 | On Loan: No      |
| Cimpress PLC                                | Cimpress PLC SHS EURO                                          | CUSIP: G2143T103<br>LEI: 549300X1IFD75XISOR81 | Long             | EC               | CORP              | US        |   2355405 | NS      | $171944565.00 | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Flex Ltd.                                   | Flex Ltd. COM USD0.01                                          | CUSIP: Y2573F102<br>LEI: 549300EAQH74YHD07T53 | Long             | EC               | CORP              | US        |  11766969 | NS      | $770265790.74 | 3.77%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-95869.02    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $97555.62     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $23324.40     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $6181.56      | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-1800996.75  | -0.01%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $1715119.03   | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $3033887.73   | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-1972984.86  | -0.01%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $699618.38    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| API Group Corp                              | API Group Corp COM STK                                         | CUSIP: 00187Y100<br>LEI: 213800NZT1VX6PZ7BT53 | Long             | EC               | CORP              | US        |  13147907 | NS      | $532753191.64 | 2.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Alliant Energy Corporation                  | Alliant Energy Corporation COM USD0.01                         | CUSIP: 018802108<br>LEI: 5493009ML300G373MZ12 | Long             | EC               | CORP              | US        |   7227173 | NS      | $518621934.48 | 2.54%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-10110.06    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $1533597.38   | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |
| Ameren Corp.                                | Ameren Corp. COM USD0.01                                       | CUSIP: 023608102<br>LEI: XRZQ5S7HYJFPHJ78L959 | Long             | EC               | CORP              | US        |   3512382 | NS      | $386081029.44 | 1.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Applovin Corp.                              | Applovin Corp. COM CL A                                        | CUSIP: 03831W108<br>LEI: 549300LLVXMUAOL3SQ07 | Long             | EC               | CORP              | US        |    311354 | NS      | $123918892.00 | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Aramark                                     | Aramark COM USD0.01                                            | CUSIP: 03852U106<br>LEI: 549300MR6PG2DWZUIL39 | Long             | EC               | CORP              | US        |  10081068 | NS      | $408686496.72 | 2.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                   | Argenx SE SPON ADR EACH REP 1 ORD SHS                          | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    309064 | NS      | $225693986.00 | 1.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                         | Ascendis Pharma A/S SPON ADR EACH REP 1 ORD SHS                | CUSIP: 04351P101<br>LEI: 549300B66JN3W3J8GH73 | Long             |  | CORP              | US        |    455042 | NS      | $104081756.66 | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| W.R. Berkley Corporation                    | W.R. Berkley Corporation COM USD0.20                           | CUSIP: 084423102<br>LEI: SQOAGCLKBDWNVYV1OV80 | Long             | EC               | CORP              | US        |   1738101 | NS      | $115201334.28 | 0.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Henry Jack & Associates Inc.                | Henry Jack & Associates Inc. COM USD0.01                       | CUSIP: 426281101<br>LEI: 529900X1QS8C54W0JB21 | Long             | EC               | CORP              | US        |    479046 | NS      | $75708429.84  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| J. B. Hunt Transport Services, Inc.         | J. B. Hunt Transport Services, Inc. COM USD0.01                | CUSIP: 445658107<br>LEI: 549300XCD1MPI1C5GK90 | Long             | EC               | CORP              | US        |   2853222 | NS      | $604597741.80 | 2.96%             |  |  |  | No            |                  1 | On Loan: No      |
| ICU Medical Inc                             | ICU Medical Inc COM USD0.10                                    | CUSIP: 44930G107<br>LEI: 5493003NRW7LS2QZRH60 | Long             | EC               | CORP              | US        |   1230753 | NS      | $158951749.95 | 0.78%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc                                | Illumina Inc COM USD0.01                                       | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |   1338491 | NS      | $164982400.66 | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Ingersoll Rand Inc                          | Ingersoll Rand Inc COM                                         | CUSIP: 45687V106<br>LEI: 5299004C02FMZCUOIR50 | Long             | EC               | CORP              | US        |   4676941 | NS      | $374716512.92 | 1.83%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                   | KLA Corp. COM USD0.001                                         | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    189323 | NS      | $278761078.43 | 1.36%             |  |  |  | No            |                  1 | On Loan: No      |
| LPL Financial Holdings Inc.                 | LPL Financial Holdings Inc. COM USD0.001                       | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |   1828994 | NS      | $550216265.02 | 2.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Lamar Advertising Company                   | Lamar Advertising Company COM USD0.001 CL A                    | CUSIP: 512816109<br>LEI: 529900Y8WW4MKVPTB543 | Long             | EC               | CORP              | US        |    840871 | NS      | $106504720.86 | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc                       | Lantheus Holdings Inc COM USD0.01                              | CUSIP: 516544103<br>LEI: 5299005HUB5IOJGO7S39 | Long             | EC               | CORP              | US        |   1132936 | NS      | $85933195.60  | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del                      | Liberty Media Corp Del COM LBTY ONE S C                        | CUSIP: 531229755<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |   4700460 | NS      | $399633109.20 | 1.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del                      | Liberty Media Corp Del COM LBTY ONE S A                        | CUSIP: 531229771<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |    731603 | NS      | $57123562.24  | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Madrigal Pharmaceuticals Inc                | Madrigal Pharmaceuticals Inc USD0.0001                         | CUSIP: 558868105<br>LEI: 549300CHIMT2EUSNJB60 | Long             | EC               | CORP              | US        |    233269 | NS      | $122109323.43 | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Medline Inc.                                | Medline Inc. COM CL A                                          | CUSIP: 58507V107<br>LEI: 984500FAN6J1P8566D43 | Long             | EC               | CORP              | US        |   2957481 | NS      | $131607904.50 | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| ON Semiconductor Corp.                      | ON Semiconductor Corp. COM USD0.01                             | CUSIP: 682189105<br>LEI: ZV20P4CNJVT8V1ZGJ064 | Long             | EC               | CORP              | US        |   7509031 | NS      | $464959199.52 | 2.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Oneok Inc.                                  | Oneok Inc. COM USD0.01                                         | CUSIP: 682680103<br>LEI: 2T3D6M0JSY48PSZI1Q41 | Long             | EC               | CORP              | US        |   2877348 | NS      | $260083485.72 | 1.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Janus Henderson Cash Liquidity Fund LLC     | Janus Henderson Cash Liquidity Fund LLC                        | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        | 796768926 | NS      | $796768925.53 | 3.90%             |  |  |  | No            |                  2 | On Loan: No      |
| Intact Financial Corp.                      | Intact Financial Corp. COM NPV                                 | CUSIP: 45823T106<br>LEI: 5493009LXGE7Y4U9OY43 | Long             | EC               | CORP              | CA        |    894652 | NS      | $162142408.63 | 0.79%             |  |  |  | No            |                  2 | On Loan: No      |
| NXP Semiconductors NV                       | NXP Semiconductors NV EUR0.20                                  | CUSIP: N6596X109<br>LEI: 724500M9BY5293JDF951 | Long             | EC               | CORP              | US        |   1800147 | NS      | $354376938.42 | 1.73%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $673621.08    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $130867.44    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $14236.40     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $3222878.37   | 0.02%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $501095.62    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $614435.71    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $3489102.38   | 0.02%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $2481588.58   | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $1130620.26   | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-634063.68   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $366329.99    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-256907.70   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-216991.71   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-43848.92    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-510861.56   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $8021.66      | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-329581.32   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $85179.81     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-121680.80   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-6486.77     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-253211.13   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-259613.33   | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-65163.31    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| TFI International Inc                       | TFI International Inc COM                                      | CUSIP: 87241L109<br>LEI: 5493007R9W7KO7SJN678 | Long             | EC               | CORP              | US        |   1889400 | NS      | $205245522.00 | 1.00%             |  |  |  | No            |                  1 | On Loan: —       |
| Teledyne Technologies Inc                   | Teledyne Technologies Inc COM USD0.01                          | CUSIP: 879360105<br>LEI: 549300VSMO9KYQWDND94 | Long             | EC               | CORP              | US        |    881987 | NS      | $533610954.87 | 2.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Teleflex Incorporated                       | Teleflex Incorporated COM USD1                                 | CUSIP: 879369106<br>LEI: UUL85LWXB4NGSWWXIQ25 | Long             | EC               | CORP              | US        |   2885870 | NS      | $345178910.70 | 1.69%             |  |  |  | No            |                  1 | On Loan: No      |
| TE Connectivity PLC                         | TE Connectivity PLC ORD SHS                                    | CUSIP: G87052109<br>LEI: 6367003TB6K484NFHE54 | Long             | EC               | CORP              | US        |    868228 | NS      | $181477016.56 | 0.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation               | Boston Scientific Corporation COM USD0.01                      | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |   5565902 | NS      | $349260350.50 | 1.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Bridgebio Pharma Inc                        | Bridgebio Pharma Inc COM USD0.001                              | CUSIP: 10806X102<br>LEI: 54930028YG89VTY2J114 | Long             | EC               | CORP              | US        |   1909659 | NS      | $141811277.34 | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadridge Financial Solutions Inc.         | Broadridge Financial Solutions Inc. COM USD0.01                | CUSIP: 11133T103<br>LEI: 549300KZDJZQ2YIHRC28 | Long             | EC               | CORP              | US        |   1279978 | NS      | $207970825.44 | 1.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Burlington Stores Inc.                      | Burlington Stores Inc. COM USD0.0001                           | CUSIP: 122017106<br>LEI: 5299003Z37DVGKKC1W09 | Long             | EC               | CORP              | US        |    546782 | NS      | $177911927.16 | 0.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Ziff Davis Inc                              | Ziff Davis Inc COM USD0.001                                    | CUSIP: 48123V102<br>LEI: 5493008OJYGLHD7MXA18 | Long             | EC               | CORP              | US        |   1581368 | NS      | $66354201.28  | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| TransUnion                                  | TransUnion COM USD0.01                                         | CUSIP: 89400J107<br>LEI: 549300ZS772LUNUMRB03 | Long             | EC               | CORP              | US        |   2085935 | NS      | $144325842.65 | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| UL Solutions Inc.                           | UL Solutions Inc. CLASS A COM SHS                              | CUSIP: 903731107<br>LEI: 549300F7CUV5ESKV9P71 | Long             | EC               | CORP              | US        |   1411452 | NS      | $120975550.92 | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                                 | Vaxcyte Inc COM                                                | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |   2269870 | NS      | $131902145.70 | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Veralto Corp.                               | Veralto Corp. COM SHS                                          | CUSIP: 92338C103<br>LEI: 635400FJE6GSOJUSNY27 | Long             | EC               | CORP              | US        |   1397076 | NS      | $123529459.92 | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Waters Corporation                          | Waters Corporation COM USD0.01                                 | CUSIP: 941848103<br>LEI: 5T547R1474YC9HOD8Q74 | Long             | EC               | CORP              | US        |   1170078 | NS      | $348449228.40 | 1.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Wayfair Inc                                 | Wayfair Inc COM USD0.001 A                                     | CUSIP: 94419L101<br>LEI: 54930027N9ZAVYFDHK53 | Long             | EC               | CORP              | US        |   2260926 | NS      | $170044244.46 | 0.83%             |  |  |  | No            |                  1 | On Loan: No      |
| WEX Inc                                     | WEX Inc COM USD0.01                                            | CUSIP: 96208T104<br>LEI: T9M5IGROL1TL1G5OV478 | Long             | EC               | CORP              | US        |   2499077 | NS      | $382458744.08 | 1.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Workday Inc                                 | Workday Inc COM USD0.001 CL A                                  | CUSIP: 98138H101<br>LEI: 549300J0DYC0N31V7G13 | Long             | EC               | CORP              | US        |   1184263 | NS      | $153859448.96 | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| Topicus Com Inc                             | Topicus Com Inc SUB VTG SHS                                    | CUSIP: 89072T102<br>LEI: N/A                  | Long             | EC               | CORP              | CA        |   1001381 | NS      | $66192371.23  | 0.32%             |  |  |  | No            |                  2 | On Loan: No      |
| Willis Towers Watson Public Limited Company | Willis Towers Watson Public Limited Company COM USD0.000304635 | CUSIP: G96629103<br>LEI: 549300WHC56FF48KL350 | Long             | EC               | CORP              | US        |    641071 | NS      | $186359339.70 | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| PTC Inc                                     | PTC Inc COM USD0.01                                            | CUSIP: 69370C100<br>LEI: 54930050SSMU8SKDJ030 | Long             | EC               | CORP              | US        |   2528600 | NS      | $360300214.00 | 1.76%             |  |  |  | No            |                  1 | On Loan: No      |
| Revvity Inc                                 | Revvity Inc COM USD1.00                                        | CUSIP: 714046109<br>LEI: 549300IKL1SDPFI7N655 | Long             | EC               | CORP              | US        |   5624564 | NS      | $492768052.04 | 2.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Praxis Precision Medicines Inc              | Praxis Precision Medicines Inc COM NEW                         | CUSIP: 74006W207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    290420 | NS      | $93570419.80  | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| RB Global Inc                               | RB Global Inc COM                                              | CUSIP: 74935Q107<br>LEI: 5493002H8U24DJXQ4886 | Long             | EC               | CORP              | US        |   2681209 | NS      | $256993882.65 | 1.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Rentokil Initial PLC                        | Rentokil Initial PLC SPON ADR REP 5 ORD GBP0.01                | CUSIP: 760125104<br>LEI: 549300VN4WV7Z6T14K68 | Long             |  | CORP              | US        |   6389737 | NS      | $201148920.76 | 0.98%             |  |  |  | No            |                  1 | On Loan: —       |
| Revolution Medicines Inc                    | Revolution Medicines Inc COM                                   | CUSIP: 76155X100<br>LEI: 549300JU3X0LBCQ65892 | Long             | EC               | CORP              | US        |    999703 | NS      | $97221116.75  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Ryanair Holdings PLC                        | Ryanair Holdings PLC ADS EACH REPR 5 ORD EUR0.01               | CUSIP: 783513203<br>LEI: 635400BR2ROC1FVEBQ56 | Long             |  | CORP              | US        |   3441065 | NS      | $198893557.00 | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| SS&C Technologies Holdings Inc.             | SS&C Technologies Holdings Inc. COM USD0.01                    | CUSIP: 78467J100<br>LEI: 529900POY8H7NPPNKK71 | Long             | EC               | CORP              | US        |   4084271 | NS      | $275974191.47 | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| StandardAero Inc.                           | StandardAero Inc. COM                                          | CUSIP: 85423L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   6085562 | NS      | $157190066.46 | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Rentokil Initial PLC                        | Rentokil Initial PLC ORD GBP0.01                               | CUSIP: G7494G105<br>LEI: 549300VN4WV7Z6T14K68 | Long             | EC               | CORP              | GB        |  16467941 | NS      | $102376044.37 | 0.50%             |  |  |  | No            |                  2 | On Loan: No      |
| Ryanair Holdings PLC                        | Ryanair Holdings PLC ORD EUR0.006                              | CUSIP: G7727C186<br>LEI: 635400BR2ROC1FVEBQ56 | Long             | EC               | CORP              | IE        |   4513508 | NS      | $127852541.19 | 0.63%             |  |  |  | No            |                  2 | On Loan: No      |
| Sensata Technologies Holding PLC            | Sensata Technologies Holding PLC COM EUR0.01                   | CUSIP: G8060N102<br>LEI: 529900MS7KFYCY9MS037 | Long             | EC               | CORP              | US        |   6977054 | NS      | $245731841.88 | 1.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Steris PLC                                  | Steris PLC ORD USD0.001                                        | CUSIP: G8473T100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    994302 | NS      | $219870001.26 | 1.08%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Royal Bank of Canada                                           | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |   2449605 | PA      | $2449605.00   | 0.01%             | 2026-04-01      | Fixed         | 3.65%                 | No            |                  2 | On Loan: No      |
| Janus Henderson Cash Collateral Fund LLC    | Janus Henderson Cash Collateral Fund LLC                       | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |   9798420 | NS      | $9798420.00   | 0.05%             |  |  |  | No            |                  2 | On Loan: No      |
| Canada Dollar Cash Balance                  | Canada Dollar Cash Balance                                     | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             |  | CA        |   1673996 | OU      | $1673995.84   | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2026-05-15

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER