# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001193125-25-301786
**Filing Date:** 2025-11
**Character Count:** 16154
**Document Hash:** 69d25f1846dfec56d5f846817393cd0b
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-301786.hdr.sgml**: 20251128

**ACCESSION NUMBER**: 0001193125-25-301786

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250930

**FILED AS OF DATE**: 20251128

**DATE AS OF CHANGE**: 20251128

**PERIOD START**: 20250930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 251534190

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Forty Fund (Series ID: S000025893)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000077618 | Class A      | JDCAX           |
| C000077619 | Class C      | JACCX           |
| C000077620 | Class I      | JCAPX           |
| C000077621 | Class R      | JDCRX           |
| C000077622 | Class S      | JARTX           |
| C000077623 | Class T      | JACTX           |
| C000114888 | Class N      | JFRNX           |
| C000177054 | Class D      | JFRDX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Forty Fund

- **b. EDGAR series identifier (if any):** S000025893

- **c. LEI of Series:** 549300RDSCGTT8PWPM78

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-09-30

- **b. Date as of which information is reported:** 2025-09-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $24594403751.58

**Total Liabilities:** $15936534.28

**Net Assets:** $24578467217.30

**Cash Not Reported:** $3826140.34

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000077618 | 4.33%                | -0.71%               | 4.55%                |
| Class ID C000077619 | 4.27%                | -0.76%               | 4.50%                |
| Class ID C000077620 | 4.36%                | -0.70%               | 4.58%                |
| Class ID C000077621 | 4.31%                | -0.76%               | 4.52%                |
| Class ID C000077622 | 4.32%                | -0.74%               | 4.53%                |
| Class ID C000077623 | 4.34%                | -0.72%               | 4.57%                |
| Class ID C000114888 | 4.36%                | -0.69%               | 4.59%                |
| Class ID C000177054 | 4.36%                | -0.69%               | 4.57%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $129995429.00            | $881850629.00                              |
| Month 2  | $13550678.00             | $-180056258.00                             |
| Month 3  | $38625857.00             | $1041917810.00                             |

**Designated Index Information**

- **Index Name:** Russell 1000 Growth Total Return Index

- **Index Identifier:** RUSSELL.MC.RLG

### Schedule of Portfolio Investments

| Name                                         | Title                                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)    | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Alphabet Inc.                                | Alphabet Inc. CAP USD0.001 CL C                                       | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |   3654001 | NS      | $889931943.55  | 3.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Amazon.com, Inc.                             | Amazon.com, Inc. COM USD0.01                                          | CUSIP: 023135106<br>LEI: ZXTILKJKG63JELOEG630 | Long             | EC               | CORP              | US        |   8821022 | NS      | $1936831800.54 | 7.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Applovin Corp.                               | Applovin Corp. COM CL A                                               | CUSIP: 03831W108<br>LEI: 549300LLVXMUAOL3SQ07 | Long             | EC               | CORP              | US        |    496687 | NS      | $356889476.98  | 1.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                                   | Apple Inc. COM USD0.00001                                             | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |   4880424 | NS      | $1242702363.12 | 5.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                    | Argenx SE SPON ADR EACH REP 1 ORD SHS                                 | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    572746 | NS      | $422434539.76  | 1.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Blackstone Inc                               | Blackstone Inc COM USD0.00001 CLASS A                                 | CUSIP: 09260D107<br>LEI: 5299004LW4QWGZUB8Y96 | Long             | EC               | CORP              | US        |   4405573 | NS      | $752692147.05  | 3.06%             |  |  |  | No            |                  1 | On Loan: No      |
| The Boeing Company                           | The Boeing Company COM USD5.00                                        | CUSIP: 097023105<br>LEI: RVHJWBXLJ1RFUBSY1F30 | Long             | EC               | CORP              | US        |   1018317 | NS      | $219783358.11  | 0.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                         | Booking Holdings Inc COM USD0.008                                     | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |    108742 | NS      | $587127418.34  | 2.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadcom Inc                                 | Broadcom Inc COM USD0.001                                             | CUSIP: 11135F101<br>LEI: 549300WV6GIDOZJTV909 | Long             | EC               | CORP              | US        |   4486096 | NS      | $1480007931.36 | 6.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Chipotle Mexican Grill Inc                   | Chipotle Mexican Grill Inc COM USD0.01                                | CUSIP: 169656105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   5872029 | NS      | $230124816.51  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                          | Danaher Corporation COM USD0.01                                       | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |   2602782 | NS      | $516027559.32  | 2.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Datadog Inc                                  | Datadog Inc COM USD0.00001 CL A                                       | CUSIP: 23804L103<br>LEI: 549300F6JNO0KRPO1K63 | Long             | EC               | CORP              | US        |   4244246 | NS      | $604380630.40  | 2.46%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                                 | DoorDash Inc CL A                                                     | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |   1490027 | NS      | $405272443.73  | 1.65%             |  |  |  | No            |                  1 | On Loan: No      |
| DraftKings Inc.                              | DraftKings Inc. COM CL A                                              | CUSIP: 26142V105<br>LEI: 2549006G33IUL6IVOD83 | Long             | EC               | CORP              | US        |  12816580 | NS      | $479340092.00  | 1.95%             |  |  |  | No            |                  1 | On Loan: No      |
| Howmet Aerospace Inc                         | Howmet Aerospace Inc COM                                              | CUSIP: 443201108<br>LEI: 549300HO5WFZUT5N2T22 | Long             | EC               | CORP              | US        |   3468022 | NS      | $680529957.06  | 2.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                              | Eli Lilly & Co. COM NPV                                               | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |    893446 | NS      | $681699298.00  | 2.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Eaton Corporation PLC                        | Eaton Corporation PLC COM USD0.01                                     | CUSIP: G29183103<br>LEI: 549300VDIGTMXUNT7H71 | Long             | EC               | CORP              | US        |   1710274 | NS      | $640070044.50  | 2.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Meta Platforms Inc                           | Meta Platforms Inc COM USD0.000006 CL 'A'                             | CUSIP: 30303M102<br>LEI: BQ4BKCS1HXDV9HN80Z93 | Long             | EC               | CORP              | US        |   1565798 | NS      | $1149890735.24 | 4.68%             |  |  |  | No            |                  1 | On Loan: No      |
| Mercadolibre Inc                             | Mercadolibre Inc COM USD0.001                                         | CUSIP: 58733R102<br>LEI: 549300DKPDN9M5S8GB14 | Long             | EC               | CORP              | US        |    223443 | NS      | $522172884.42  | 2.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp.                              | Microsoft Corp. COM USD0.00000625                                     | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |   5444807 | NS      | $2820137785.65 | 11.47%            |  |  |  | No            |                  1 | On Loan: No      |
| Monster Beverage Corp                        | Monster Beverage Corp COM USD0.005                                    | CUSIP: 61174X109<br>LEI: 52990090AP0E7HCB6F33 | Long             | EC               | CORP              | US        |   7239388 | NS      | $487283206.28  | 1.98%             |  |  |  | No            |                  1 | On Loan: No      |
| NVIDIA Corp.                                 | NVIDIA Corp. COM USD0.001                                             | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |  13898206 | NS      | $2593127275.48 | 10.55%            |  |  |  | No            |                  1 | On Loan: No      |
| Oracle Corp.                                 | Oracle Corp. COM USD0.01                                              | CUSIP: 68389X105<br>LEI: 1Z4GXXU7ZHVWFCD8TV52 | Long             | EC               | CORP              | US        |   4690914 | NS      | $1319272653.36 | 5.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Shopify Inc                                  | Shopify Inc COM NPV CL A                                              | CUSIP: 82509L107<br>LEI: 549300HGQ43STJLLP808 | Long             | EC               | CORP              | US        |   2876686 | NS      | $427504306.46  | 1.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Co., Ltd. | Taiwan Semiconductor Manufacturing Co., Ltd. SPON ADS REP 5 ORD TWD10 | CUSIP: 874039100<br>LEI: 549300KB6NK5SBD14S87 | Long             |  | CORP              | US        |   3163788 | NS      | $883614350.52  | 3.60%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Inc.                      | UnitedHealth Group Inc. COM USD0.01                                   | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |   1390630 | NS      | $480184539.00  | 1.95%             |  |  |  | No            |                  1 | On Loan: No      |
| Hubspot Inc                                  | Hubspot Inc COM USD0.001                                              | CUSIP: 443573100<br>LEI: 529900BLNVEW0MVKJB37 | Long             | EC               | CORP              | US        |    504413 | NS      | $235964401.40  | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Intercontinental Exchange Inc                | Intercontinental Exchange Inc COM USD0.01                             | CUSIP: 45866F104<br>LEI: 5493000F4ZO33MV32P92 | Long             | EC               | CORP              | US        |   1714887 | NS      | $288924161.76  | 1.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Madrigal Pharmaceuticals Inc                 | Madrigal Pharmaceuticals Inc USD0.0001                                | CUSIP: 558868105<br>LEI: 549300CHIMT2EUSNJB60 | Long             | EC               | CORP              | US        |    522050 | NS      | $239443453.00  | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| MasterCard Incorporated                      | MasterCard Incorporated COM USD0.0001 CLASS A                         | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |   1535043 | NS      | $873147808.83  | 3.55%             |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC                      | Janus Henderson Cash Liquidity Fund LLC                               | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        | 140492883 | NS      | $140520981.19  | 0.57%             |  |  |  | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2025-11-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER