# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001193125-25-301791
**Filing Date:** 2025-11
**Character Count:** 42227
**Document Hash:** fa40d2b68913ec4c9b808bc59aed5d8b
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-301791.hdr.sgml**: 20251128

**ACCESSION NUMBER**: 0001193125-25-301791

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250930

**FILED AS OF DATE**: 20251128

**DATE AS OF CHANGE**: 20251128

**PERIOD START**: 20250930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 251534195

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Global Life Sciences Fund (Series ID: S000010494)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028956 | Class T      | JAGLX           |
| C000077752 | Class A      | JFNAX           |
| C000077753 | Class C      | JFNCX           |
| C000077754 | Class I      | JFNIX           |
| C000077755 | Class S      | JFNSX           |
| C000083538 | Class D      | JNGLX           |
| C000198286 | Class N      | JFNNX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Global Life Sciences Fund

- **b. EDGAR series identifier (if any):** S000010494

- **c. LEI of Series:** 5493001MPP2BKRJEGV73

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-09-30

- **b. Date as of which information is reported:** 2025-09-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $4581920396.81

**Total Liabilities:** $8932867.29

**Net Assets:** $4572987529.52

**Cash Not Reported:** $71.93

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028956 | -0.87%               | 6.13%                | 2.87%                |
| Class ID C000077752 | -0.87%               | 6.12%                | 2.87%                |
| Class ID C000077753 | -0.94%               | 6.05%                | 2.81%                |
| Class ID C000077754 | -0.86%               | 6.13%                | 2.89%                |
| Class ID C000077755 | -0.90%               | 6.10%                | 2.85%                |
| Class ID C000083538 | -0.85%               | 6.13%                | 2.88%                |
| Class ID C000198286 | -0.85%               | 6.14%                | 2.90%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $20682463.00             | $-56663223.00                              |
| Month 2  | $55863307.00             | $203656517.00                              |
| Month 3  | $31975309.00             | $94723099.00                               |

**Designated Index Information**

- **Index Name:** MSCI THE WORLD INDEX / Health Care

- **Index Identifier:** MSCI.MC.MXWO0HC

### Schedule of Portfolio Investments

| Name                                          | Title                                                           | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Cencora Inc                                   | Cencora Inc COM USD0.01                                         | CUSIP: 03073E105<br>LEI: AI8GXW8LG5WK7E9UD086 | Long             | EC               | CORP              | US        |    164570 | NS      | $51433062.10  | 1.12%             |  |  |  | No            |                  1 | On Loan: No      |
| CVS Health Corp                               | CVS Health Corp COM USD0.01                                     | CUSIP: 126650100<br>LEI: 549300EJG376EN5NQE29 | Long             | EC               | CORP              | US        |    998078 | NS      | $75245100.42  | 1.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Centene Corp.                                 | Centene Corp. COM USD0.001                                      | CUSIP: 15135B101<br>LEI: 549300Z7JJ4TQSQGT333 | Long             | EC               | CORP              | US        |    345196 | NS      | $12316593.28  | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cooper Companies, Inc.                    | The Cooper Companies, Inc. COM                                  | CUSIP: 216648501<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |    475586 | NS      | $32606176.16  | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Abbott Laboratories                           | Abbott Laboratories COM NPV                                     | CUSIP: 002824100<br>LEI: HQD377W2YR662HK5JX27 | Long             | EC               | CORP              | US        |   1079904 | NS      | $144642341.76 | 3.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Mirum Pharmaceuticals Inc.                    | Mirum Pharmaceuticals Inc. COM USD0.0001                        | CUSIP: 604749101<br>LEI: 254900YZKR40F7F27K81 | Long             | EC               | CORP              | US        |    654790 | NS      | $48002654.90  | 1.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Neurocrine Biosciences Inc                    | Neurocrine Biosciences Inc COM USD0.001                         | CUSIP: 64125C109<br>LEI: 549300FECER0XBN49756 | Long             | EC               | CORP              | US        |    124275 | NS      | $17445724.50  | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                   | AbbVie Inc. COM USD0.01                                         | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |    706535 | NS      | $163591113.90 | 3.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Akero Therapeutics Inc                        | Akero Therapeutics Inc COM USD0.0001                            | CUSIP: 00973Y108<br>LEI: 549300SI3KG74LBE2955 | Long             | EC               | CORP              | US        |   1289433 | NS      | $61222278.84  | 1.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Align Technology Inc.                         | Align Technology Inc. COM USD0.0001                             | CUSIP: 016255101<br>LEI: 549300N5YSE0U3LCP840 | Long             | EC               | CORP              | US        |     92443 | NS      | $11575712.46  | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Alnylam Pharmaceuticals Inc.                  | Alnylam Pharmaceuticals Inc. COM USD0.01                        | CUSIP: 02043Q107<br>LEI: 529900S3ZI14OWRJII50 | Long             | EC               | CORP              | US        |     48877 | NS      | $22287912.00  | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                                    | Amgen Inc. COM USD0.0001                                        | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |    362293 | NS      | $102239084.60 | 2.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Amicus Therapeutics Inc.                      | Amicus Therapeutics Inc. COM STK USD0.01                        | CUSIP: 03152W109<br>LEI: 549300MRD1SMUTNBPQ53 | Long             | EC               | CORP              | US        |   1009777 | NS      | $7957042.76   | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Elevance Health Inc.                          | Elevance Health Inc. COM USD0.01                                | CUSIP: 036752103<br>LEI: 8MYN82XMYQH89CTMTH67 | Long             | EC               | CORP              | US        |     99500 | NS      | $32150440.00  | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Apogee Therapeutics Inc.                      | Apogee Therapeutics Inc. COM                                    | CUSIP: 03770N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    205848 | NS      | $8178341.04   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcellx Inc                                   | Arcellx Inc COMMON STOCK                                        | CUSIP: 03940C100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    244065 | NS      | $20037736.50  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Ardelyx Inc                                   | Ardelyx Inc COM USD0.0001                                       | CUSIP: 039697107<br>LEI: 549300F542QR4SXHCY32 | Long             | EC               | CORP              | US        |   3146321 | NS      | $17336228.71  | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                     | Argenx SE SPON ADR EACH REP 1 ORD SHS                           | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    175105 | NS      | $129150443.80 | 2.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                           | Ascendis Pharma A/S SPON ADR EACH REP 1 ORD SHS                 | CUSIP: 04351P101<br>LEI: 549300B66JN3W3J8GH73 | Long             |  | CORP              | US        |    382422 | NS      | $76029317.82  | 1.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.                      | Avidity Biosciences Inc. COM                                    | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1656561 | NS      | $72176362.77  | 1.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Becton, Dickinson and Company                 | Becton, Dickinson and Company COM USD1.00                       | CUSIP: 075887109<br>LEI: ICE2EP6D98PQUILVRZ91 | Long             | EC               | CORP              | US        |    167825 | NS      | $31411805.25  | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                               | AstraZeneca PLC ORD USD0.25                                     | CUSIP: G0593M107<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |   1264267 | NS      | $193092382.16 | 4.22%             |  |  |  | No            |                  2 | On Loan: No      |
| Boston Scientific Corporation                 | Boston Scientific Corporation COM USD0.01                       | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |   1285401 | NS      | $125493699.63 | 2.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Bridgebio Pharma Inc                          | Bridgebio Pharma Inc COM USD0.001                               | CUSIP: 10806X102<br>LEI: 54930028YG89VTY2J114 | Long             | EC               | CORP              | US        |   1502856 | NS      | $78058340.64  | 1.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Crinetics Pharmaceuticals Inc.                | Crinetics Pharmaceuticals Inc. COM USD0.001                     | CUSIP: 22663K107<br>LEI: 549300Q1CNFXK5P31D28 | Long             | EC               | CORP              | US        |    304704 | NS      | $12690921.60  | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                           | Danaher Corporation COM USD0.01                                 | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |    424559 | NS      | $84173067.34  | 1.84%             |  |  |  | No            |                  1 | On Loan: No      |
| DexCom Inc                                    | DexCom Inc COM USD0.001                                         | CUSIP: 252131107<br>LEI: 549300YSK3QDSFR5EU59 | Long             | EC               | CORP              | US        |    654827 | NS      | $44063308.83  | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Dyne Therapeutics Inc.                        | Dyne Therapeutics Inc. COM                                      | CUSIP: 26818M108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2858143 | NS      | $36155508.95  | 0.79%             |  |  |  | No            |                  1 | On Loan: No      |
| Edgewise Therapeutics Inc.                    | Edgewise Therapeutics Inc. COM                                  | CUSIP: 28036F105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    724994 | NS      | $11759402.68  | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Edwards Lifesciences Corporation              | Edwards Lifesciences Corporation COM USD1                       | CUSIP: 28176E108<br>LEI: YA13X31F3V31L8TMPR58 | Long             | EC               | CORP              | US        |    463525 | NS      | $36048339.25  | 0.79%             |  |  |  | No            |                  1 | On Loan: No      |
| 89bio Inc.                                    | 89bio Inc. COM                                                  | CUSIP: 282559103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    199694 | NS      | $2935501.80   | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| KARDIUM SERIES 8 CLASS D PREFERRED SHARES USD | HeartFlow Inc                                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |    145275 | NS      | $4889956.50   | 0.01%             |  |  |  | Yes           |                  1 | On Loan: No      |
| Green Cross Holdings Corp                     | Curevo Inc                                                      | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   2031087 | NS      | $5330344.10   | 0.12%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Element Biosciences Inc                       | Element Biosciences Inc                                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    425023 | NS      | $2461308.19   | 0.05%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Sonoma Biotherapeutics Inc                    | Sonoma Biotherapeutics Inc - Series B-1                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    669428 | NS      | $1051001.96   | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Loadsmart Inc                                 | Curevo Inc - Series B                                           | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    674104 | NS      | $1769105.06   | 0.04%             |  |  |  | Yes           |                  3 | On Loan: No      |
| KARDIUM SERIES 8 CLASS D PREFERRED SHARES USD | Kardium Inc - Series 8 Class D Preferred shares                 | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | CA        |  13234820 | NS      | $12237311.62  | 0.27%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Magnolia Medical Technologies                 | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |         1 | NS      | $0.00         | 0.00%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Shoreline Biosciences Inc                     | Shoreline Biosciences Inc                                       | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    747187 | NS      | $164381.14    | 0.00%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Hemoshear Therapeutics LLC                    | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    301933 | NS      | $411789.81    | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Attralus Inc                                  | Attralus Inc                                                    | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    669935 | NS      | $1112092.10   | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Sonoma Biotherapeutics Inc                    | Sonoma Biotherapeutics Inc - Series B                           | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1255200 | NS      | $1970664.00   | 0.04%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Green Cross Holdings Corp                     | Curevo Inc - Series B-1                                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    886774 | NS      | $2327231.95   | 0.05%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Freenome Holdings Inc                         | Freenome Holdings Inc - Series D                                | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    342803 | NS      | $1570037.74   | 0.03%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Asher Biotherapeutics Inc                     | Asher Biotherapeutics - Series C                                | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   2580733 | NS      | $1619926.10   | 0.04%             |  |  |  | Yes           |                  3 | On Loan: No      |
| HeartFlow Inc                                 | HeartFlow Inc - Series F                                        | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |    662334 | NS      | $22294162.44  | 0.49%             |  |  |  | Yes           |                  1 | On Loan: No      |
| Twinstrand Biosciences Inc                    | TwinStrand Biosciences Inc                                      | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    344314 | NS      | $1380526.98   | 0.03%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Magnolia Medical Technologies                 | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1821717 | NS      | $3096918.90   | 0.07%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Gilead Sciences Inc.                          | Gilead Sciences Inc. COM USD0.001                               | CUSIP: 375558103<br>LEI: 549300WTZWR07K8MNV44 | Long             | EC               | CORP              | US        |    475138 | NS      | $52740318.00  | 1.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Globus Med Inc                                | Globus Med Inc COM USD0.001 CL 'A'                              | CUSIP: 379577208<br>LEI: 5299003M2P4CIK0RF205 | Long             | EC               | CORP              | US        |    748396 | NS      | $42860638.92  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Asher Biotherapeutics Inc                     | Asher Biotherapeutics Inc                                       | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1214301 | NS      | $762216.74    | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Freenome Holdings Inc                         | Freenome Holdings Inc - Series C                                | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    337474 | NS      | $1542256.18   | 0.03%             |  |  |  | Yes           |                  3 | On Loan: No      |
| HCA Healthcare Inc                            | HCA Healthcare Inc COM USD0.01                                  | CUSIP: 40412C101<br>LEI: 529900PH4ZGUH2MNEU89 | Long             | EC               | CORP              | US        |    228288 | NS      | $97296345.60  | 2.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Humana Inc.                                   | Humana Inc. COM USD0.166                                        | CUSIP: 444859102<br>LEI: 529900YLDW34GJAO4J06 | Long             | EC               | CORP              | US        |     64704 | NS      | $16834039.68  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEAYA Biosciences Inc.                       | IDEAYA Biosciences Inc. COM USD0.0001                           | CUSIP: 45166A102<br>LEI: 549300ULW08F62IJML11 | Long             | EC               | CORP              | US        |    767758 | NS      | $20890695.18  | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc                                  | Illumina Inc COM USD0.01                                        | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |    103520 | NS      | $9831294.40   | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunome Inc                                  | Immunome Inc COM                                                | CUSIP: 45257U108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    349668 | NS      | $4094612.28   | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Insmed Inc                                    | Insmed Inc COM USD0.01                                          | CUSIP: 457669307<br>LEI: 529900Q55QAG41CCAC11 | Long             | EC               | CORP              | US        |    144837 | NS      | $20857976.37  | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Inspire Medical Systems Inc                   | Inspire Medical Systems Inc COM USD0.001                        | CUSIP: 457730109<br>LEI: 549300XQQCQ7HCJKTW25 | Long             | EC               | CORP              | US        |    148268 | NS      | $11001485.60  | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                       | Intuitive Surgical Inc. COM USD0.001                            | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |    219297 | NS      | $98076197.31  | 2.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Janux Therapeutics Inc                        | Janux Therapeutics Inc COM                                      | CUSIP: 47103J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    482882 | NS      | $11801636.08  | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Johnson & Johnson                             | Johnson & Johnson COM USD1.00                                   | CUSIP: 478160104<br>LEI: 549300G0CFPGEF6X2043 | Long             | EC               | CORP              | US        |   1235890 | NS      | $229158723.80 | 5.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc                         | Lantheus Holdings Inc COM USD0.01                               | CUSIP: 516544103<br>LEI: 5299005HUB5IOJGO7S39 | Long             | EC               | CORP              | US        |    544082 | NS      | $27905965.78  | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Legend Biotech Corporation                    | Legend Biotech Corporation SPONSORED ADS                        | CUSIP: 52490G102<br>LEI: 254900EIUFZTW136H859 | Long             |  | CORP              | US        |    437283 | NS      | $14259798.63  | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Lexeo Therapeutics Inc                        | Lexeo Therapeutics Inc COM                                      | CUSIP: 52886X107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    642299 | NS      | $4264865.36   | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                               | Eli Lilly & Co. COM NPV                                         | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |    497962 | NS      | $379945006.00 | 8.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Novartis AG                                   | Novartis AG ADR-REP 1 CHF0.5 (REGD)                             | CUSIP: 66987V109<br>LEI: 5493007HIVTX6SY6XD66 | Long             |  | CORP              | US        |    914366 | NS      | $117258295.84 | 2.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvalent Inc                                  | Nuvalent Inc COM                                                | CUSIP: 670703107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    302812 | NS      | $26187181.76  | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Inc.                       | UnitedHealth Group Inc. COM USD0.01                             | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |    556891 | NS      | $192294462.30 | 4.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                                   | Vaxcyte Inc COM                                                 | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |    998056 | NS      | $35949977.12  | 0.79%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated           | Vertex Pharmaceuticals Incorporated COM USD0.01                 | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |    243863 | NS      | $95506505.32  | 2.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Xenon Pharmaceuticals Inc                     | Xenon Pharmaceuticals Inc COM NPV                               | CUSIP: 98420N105<br>LEI: 549300XV44Q9Z1MIPQ03 | Long             | EC               | CORP              | US        |    317935 | NS      | $12765090.25  | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Madrigal Pharmaceuticals Inc                  | Madrigal Pharmaceuticals Inc USD0.0001                          | CUSIP: 558868105<br>LEI: 549300CHIMT2EUSNJB60 | Long             | EC               | CORP              | US        |    149654 | NS      | $68640303.64  | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Sanofi SA                                     | Sanofi SA EUR2                                                  | CUSIP: F5548N101<br>LEI: 549300E9PC51EN656011 | Long             | EC               | CORP              | FR        |   1300755 | NS      | $122937329.26 | 2.69%             |  |  |  | No            |                  2 | On Loan: No      |
| Mckesson Corp.                                | Mckesson Corp. COM USD0.01                                      | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |     85319 | NS      | $65912340.26  | 1.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Metsera Inc.                                  | Metsera Inc. COM                                                | CUSIP: 59267L107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    585969 | NS      | $30663757.77  | 0.67%             |  |  |  | No            |                  1 | On Loan: —       |
| PTC Therapeutics, Inc.                        | PTC Therapeutics, Inc. COM USD0.001                             | CUSIP: 69366J200<br>LEI: 549300UJLWOIWFDGB318 | Long             | EC               | CORP              | US        |    712301 | NS      | $43713912.37  | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Penumbra Inc.                                 | Penumbra Inc. COM USD0.001                                      | CUSIP: 70975L107<br>LEI: 549300RGE06P3ECANU72 | Long             | EC               | CORP              | US        |     93774 | NS      | $23754829.68  | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Roche Holding AG                              | Roche Holding AG GENUSSCHEINE NPV                               | CUSIP: H69293217<br>LEI: 549300U41AUUVOAAOB37 | Long             | EP               | CORP              | CH        |    235574 | NS      | $77549377.31  | 1.70%             |  |  |  | No            |                  2 | On Loan: No      |
| Praxis Precision Medicines Inc                | Praxis Precision Medicines Inc COM NEW                          | CUSIP: 74006W207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    382496 | NS      | $20272288.00  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc                      | Revolution Medicines Inc COM                                    | CUSIP: 76155X100<br>LEI: 549300JU3X0LBCQ65892 | Long             | EC               | CORP              | US        |   1321139 | NS      | $61697191.30  | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Rhythm Pharmaceuticals Inc                    | Rhythm Pharmaceuticals Inc COM USD0.001                         | CUSIP: 76243J105<br>LEI: 529900JPNWXQ8DPLA551 | Long             | EC               | CORP              | US        |    220147 | NS      | $22232645.53  | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Scholar Rock Holding Corp.                    | Scholar Rock Holding Corp. COM USD0.001                         | CUSIP: 80706P103<br>LEI: 549300Z1FLPGL4OWJ459 | Long             | EC               | CORP              | US        |    360796 | NS      | $13436043.04  | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Soleno Therapeutics Inc                       | Soleno Therapeutics Inc COM                                     | CUSIP: 834203309<br>LEI: 5493003LCY77N7FXK844 | Long             | EC               | CORP              | US        |   1015340 | NS      | $68636984.00  | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Sotera Health Company                         | Sotera Health Company COM                                       | CUSIP: 83601L102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1071478 | NS      | $16854348.94  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Stryker Corporation                           | Stryker Corporation COM USD0.10                                 | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |    225946 | NS      | $83525457.82  | 1.83%             |  |  |  | No            |                  1 | On Loan: No      |
| Structure Therapeutics Inc                    | Structure Therapeutics Inc SPONSORED ADS                        | CUSIP: 86366E106<br>LEI: N/A                  | Long             |  | CORP              | US        |    491016 | NS      | $13748448.00  | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Tarsus Pharmaceuticals Inc                    | Tarsus Pharmaceuticals Inc COM                                  | CUSIP: 87650L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    298519 | NS      | $17740984.17  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Teleflex Incorporated                         | Teleflex Incorporated COM USD1                                  | CUSIP: 879369106<br>LEI: UUL85LWXB4NGSWWXIQ25 | Long             | EC               | CORP              | US        |    102708 | NS      | $12567350.88  | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Teva Pharmaceutical Industries Ltd.           | Teva Pharmaceutical Industries Ltd. SPON ADS EACH REP 1 ORD SHS | CUSIP: 881624209<br>LEI: 549300BAFGM4RC74ZJ94 | Long             |  | CORP              | US        |   2078509 | NS      | $41985881.80  | 0.92%             |  |  |  | No            |                  1 | On Loan: No      |
| Thermo Fisher Scientific Inc                  | Thermo Fisher Scientific Inc COM USD1                           | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |    216413 | NS      | $104964633.26 | 2.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Travere Therapeutics Inc                      | Travere Therapeutics Inc COM                                    | CUSIP: 89422G107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1616705 | NS      | $38639249.50  | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC                       | Janus Henderson Cash Liquidity Fund LLC                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |  30056283 | NS      | $30062294.12  | 0.66%             |  |  |  | No            |                  2 | On Loan: No      |
| United Therapeutics Corporation               | United Therapeutics Corporation COM USD0.01                     | CUSIP: 91307C102<br>LEI: 5299005C4HZL4UWROC14 | Long             | EC               | CORP              | US        |     50486 | NS      | $21164236.06  | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Daiichi Sankyo Co Ltd.                        | Daiichi Sankyo Co Ltd. NPV                                      | CUSIP: J11257102<br>LEI: 353800XGIU2IHQGC9504 | Long             | EC               | CORP              | JP        |   1708900 | NS      | $38253082.67  | 0.84%             |  |  |  | No            |                  2 | On Loan: No      |
| Biohaven Ltd                                  | Biohaven Ltd COM                                                | CUSIP: G1110E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    898042 | NS      | $13479610.42  | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Zai Lab Ltd.                                  | Zai Lab Ltd. ADS EACH REP 1 ORD SHS                             | CUSIP: 98887Q104<br>LEI: 549300P2UYQ9U5LY1T58 | Long             |  | CORP              | US        |    579330 | NS      | $19633493.70  | 0.43%             |  |  |  | No            |                  1 | On Loan: —       |
| Zoetis Inc.                                   | Zoetis Inc. COM USD0.01 CL 'A'                                  | CUSIP: 98978V103<br>LEI: 549300HD9Q1LOC9KLJ48 | Long             | EC               | CORP              | US        |    144275 | NS      | $21110318.00  | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Avadel Pharmaceuticals PLC                    | Avadel Pharmaceuticals PLC COM SHS                              | CUSIP: G29687103<br>LEI: 549300E27PTSG2RHC762 | Long             | EC               | CORP              | US        |   1503297 | NS      | $22955345.19  | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Medtronic PLC                                 | Medtronic PLC USD0.0001                                         | CUSIP: G5960L103<br>LEI: 549300GX3ZBSQWUXY261 | Long             | EC               | CORP              | US        |    795435 | NS      | $75757229.40  | 1.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Steris PLC                                    | Steris PLC ORD USD0.001                                         | CUSIP: G8473T100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     82962 | NS      | $20528117.28  | 0.45%             |  |  |  | No            |                  1 | On Loan: No      |
| 4D Molecular Therapeutics Inc                 | 4D Molecular Therapeutics Inc COM                               | CUSIP: 35104E100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    851188 | NS      | $7396823.72   | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Magnolia Medical Technologies                 | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |     37748 | NS      | $37.75        | 0.00%             |  |  |  | Yes           |                  3 | On Loan: No      |
| N/A                                           | Royal Bank of Canada                                            | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |   2826761 | PA      | $2826761.00   | 0.06%             | 2025-10-01      | Fixed         | 4.31%                 | No            |                  2 | On Loan: No      |
| Janus Henderson Cash Collateral Fund          | Janus Henderson Cash Collateral Fund                            | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |  11307042 | NS      | $11307042.00  | 0.25%             |  |  |  | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2025-11-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER