# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001741773-23-000778
**Filing Date:** 2023-3
**Character Count:** 65628
**Document Hash:** e3b6d1a9a9762f65f956cfc06992b876
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001741773-23-000778.hdr.sgml**: 20230301

**ACCESSION NUMBER**: 0001741773-23-000778

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 2

**CONFORMED PERIOD OF REPORT**: 20221231

**FILED AS OF DATE**: 20230301

**DATE AS OF CHANGE**: 20230301

**PERIOD START**: 20230930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751
- **IRS NUMBER:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 23691530

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Global Research Fund (Series ID: S000010489)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028951 | Class T      | JAWWX           |
| C000077732 | Class A      | JDWAX           |
| C000077733 | Class C      | JWWCX           |
| C000077734 | Class I      | JWWFX           |
| C000077735 | Class R      | JDWRX           |
| C000077736 | Class S      | JWGRX           |
| C000083534 | Class D      | JANWX           |
| C000114882 | Class N      | JDWNX           |

## Nport-Ex

### Janus Henderson Global Research Fund

#### Schedule of Investments (unaudited)

#### December 31, 2022

---

| | | |
|:---|:---|:---|
| *Shares or*<br>*Principal Amounts* | *Shares or*<br>*Principal Amounts* | *Value* |
| Common Stocks– 98.8% | Common Stocks– 98.8% |  |
| Aerospace & Defense – 2.0% | Aerospace & Defense – 2.0% |  |
| Airbus SE | 293174 | $34854091 |
| General Dynamics Corp | 77784 | 19298988 |
|  |  | 54153079 |
| Air Freight & Logistics – 1.7% | Air Freight & Logistics – 1.7% |  |
| United Parcel Service Inc | 259566 | 45122953 |
| Airlines – 0.4% | Airlines – 0.4% |  |
| Ryanair Holdings PLC (ADR)\* | 149509 | 11177293 |
| Automobiles – 0.4% | Automobiles – 0.4% |  |
| Tesla Inc\* | 83540 | 10290457 |
| Banks – 6.1% | Banks – 6.1% |  |
| Bank of America Corp | 870378 | 28826919 |
| BNP Paribas SA | 420730 | 23939211 |
| HDFC Bank Ltd | 583681 | 11490644 |
| JPMorgan Chase & Co | 443128 | 59423465 |
| Natwest Group PLC | 6173047 | 19701024 |
| Toronto-Dominion Bank/The | 346728 | 22453571 |
|  |  | 165834834 |
| Beverages – 3.8% | Beverages – 3.8% |  |
| Constellation Brands Inc | 226174 | 52415825 |
| Pernod Ricard SA | 262554 | 51600155 |
|  |  | 104015980 |
| Biotechnology – 3.7% | Biotechnology – 3.7% |  |
| AbbVie Inc | 251343 | 40619542 |
| Ascendis Pharma A/S (ADR)\*<sup>,#</sup> | 61740 | 7540306 |
| Horizon Therapeutics PLC\* | 141824 | 16139571 |
| Sarepta Therapeutics Inc\* | 103442 | 13404014 |
| Vertex Pharmaceuticals Inc\* | 77891 | 22493363 |
|  |  | 100196796 |
| Capital Markets – 3.9% | Capital Markets – 3.9% |  |
| Blackstone Group Inc | 211568 | 15696230 |
| Charles Schwab Corp | 340654 | 28362852 |
| London Stock Exchange Group PLC | 95814 | 8265461 |
| LPL Financial Holdings Inc | 83707 | 18094942 |
| Morgan Stanley | 291700 | 24800334 |
| State Street Corp | 142126 | 11024714 |
|  |  | 106244533 |
| Chemicals – 1.7% | Chemicals – 1.7% |  |
| Linde PLC | 145544 | 47473542 |
| Consumer Finance – 0.9% | Consumer Finance – 0.9% |  |
| Capital One Financial Corp | 177184 | 16471025 |
| OneMain Holdings Inc | 235056 | 7829715 |
|  |  | 24300740 |
| Diversified Financial Services – 0.8% | Diversified Financial Services – 0.8% |  |
| Apollo Global Management Inc | 319949 | 20409547 |
| Electric Utilities – 0.5% | Electric Utilities – 0.5% |  |
| NextEra Energy Inc | 165242 | 13814231 |
| Electronic Equipment, Instruments & Components – 1.1% | Electronic Equipment, Instruments & Components – 1.1% |  |
| Hexagon AB - Class B | 2879277 | 30282962 |
| Entertainment – 1.9% | Entertainment – 1.9% |  |
| Liberty Media Corp-Liberty Formula One\* | 487382 | 29135696 |
| Nintendo Co Ltd | 547800 | 22942952 |
|  |  | 52078648 |
| Equity Real Estate Investment Trusts (REITs) – 0.8% | Equity Real Estate Investment Trusts (REITs) – 0.8% |  |
| American Tower Corp | 102742 | 21766920 |
| Health Care Equipment & Supplies – 1.5% | Health Care Equipment & Supplies – 1.5% |  |
| Abbott Laboratories | 95751 | 10512502 |
| Boston Scientific Corp\* | 369139 | 17080062 |
| Dentsply Sirona Inc | 197822 | 6298652 |
| Edwards Lifesciences Corp\* | 102490 | 7646779 |
|  |  | 41537995 |
| Health Care Providers & Services – 1.9% | Health Care Providers & Services – 1.9% |  |
| Centene Corp\* | 225420 | 18486694 |
| Humana Inc | 42265 | 21647710 |
| UnitedHealth Group Inc | 19534 | 10356536 |
|  |  | 50490940 |
| Hotels, Restaurants & Leisure – 3.5% | Hotels, Restaurants & Leisure – 3.5% |  |
| Booking Holdings Inc\* | 15319 | 30872074 |
| Entain PLC | 1511045 | 24222456 |

---

------

---

| | | |
|:---|:---|:---|
| *Shares or*<br>*Principal Amounts* | *Shares or*<br>*Principal Amounts* | *Value* |
| Common Stocks– (continued) | Common Stocks– (continued) |  |
| Hotels, Restaurants & Leisure– (continued) | Hotels, Restaurants & Leisure– (continued) |  |
| McDonald's Corp | 154612 | $40744900 |
|  |  | 95839430 |
| Independent Power and Renewable Electricity Producers – 1.6% | Independent Power and Renewable Electricity Producers – 1.6% |  |
| RWE AG | 208825 | 9284456 |
| Vistra Energy Corp | 1447152 | 33573926 |
|  |  | 42858382 |
| Information Technology Services – 4.2% | Information Technology Services – 4.2% |  |
| Fidelity National Information Services Inc | 156364 | 10609297 |
| Global Payments Inc | 110897 | 11014290 |
| Mastercard Inc | 138723 | 48238149 |
| Visa Inc | 212081 | 44061949 |
|  |  | 113923685 |
| Insurance – 3.0% | Insurance – 3.0% |  |
| AIA Group Ltd | 1878100 | 20692228 |
| Aon PLC - Class A | 62299 | 18698422 |
| Beazley PLC | 898163 | 7349698 |
| Intact Financial Corp | 61336 | 8830699 |
| Progressive Corp/The | 205332 | 26633614 |
|  |  | 82204661 |
| Interactive Media & Services – 2.9% | Interactive Media & Services – 2.9% |  |
| Alphabet Inc - Class C\* | 824575 | 73164540 |
| Tencent Holdings Ltd | 141800 | 6027160 |
|  |  | 79191700 |
| Internet & Direct Marketing Retail – 2.5% | Internet & Direct Marketing Retail – 2.5% |  |
| Amazon.com Inc\* | 657705 | 55247220 |
| JD.Com Inc - Class A | 464128 | 13047550 |
|  |  | 68294770 |
| Life Sciences Tools & Services – 1.3% | Life Sciences Tools & Services – 1.3% |  |
| Danaher Corp | 48983 | 13001068 |
| Thermo Fisher Scientific Inc | 41340 | 22765525 |
|  |  | 35766593 |
| Machinery – 4.5% | Machinery – 4.5% |  |
| Alstom SA | 998656 | 24515872 |
| Atlas Copco AB - Class A | 2769172 | 32803296 |
| Deere & Co | 86332 | 37015708 |
| Parker-Hannifin Corp | 96048 | 27949968 |
|  |  | 122284844 |
| Metals & Mining – 2.7% | Metals & Mining – 2.7% |  |
| Freeport-McMoRan Inc | 555120 | 21094560 |
| Rio Tinto PLC | 279161 | 19583503 |
| Teck Resources Ltd | 837138 | 31641566 |
|  |  | 72319629 |
| Oil, Gas & Consumable Fuels – 6.4% | Oil, Gas & Consumable Fuels – 6.4% |  |
| Canadian Natural Resources Ltd | 582543 | 32354416 |
| Cheniere Energy Inc | 74560 | 11181018 |
| ConocoPhillips | 284192 | 33534656 |
| EOG Resources Inc | 205646 | 26635270 |
| Marathon Petroleum Corp | 288737 | 33606099 |
| Suncor Energy Inc | 732390 | 23235449 |
| Total SE | 228949 | 14287853 |
|  |  | 174834761 |
| Personal Products – 1.9% | Personal Products – 1.9% |  |
| Unilever PLC | 1015250 | 50939414 |
| Pharmaceuticals – 6.3% | Pharmaceuticals – 6.3% |  |
| AstraZeneca PLC | 325735 | 44197840 |
| Catalent Inc\* | 121604 | 5473396 |
| Eli Lilly & Co | 35956 | 13154143 |
| Merck & Co Inc | 299819 | 33264918 |
| Novartis AG | 300287 | 27207747 |
| Organon & Co | 244701 | 6834499 |
| Roche Holding AG | 70835 | 22266758 |
| Sanofi | 184495 | 17849310 |
|  |  | 170248611 |
| Road & Rail – 0.6% | Road & Rail – 0.6% |  |
| Uber Technologies Inc\* | 677577 | 16756479 |
| Semiconductor & Semiconductor Equipment – 6.0% | Semiconductor & Semiconductor Equipment – 6.0% |  |
| Advanced Micro Devices Inc\* | 203414 | 13175125 |
| ASML Holding NV | 110966 | 60057671 |
| Lam Research Corp | 28434 | 11950810 |
| Marvell Technology Inc | 291301 | 10789789 |
| NVIDIA Corp | 182561 | 26679465 |
| Taiwan Semiconductor Manufacturing Co Ltd | 1721000 | 25085827 |

---

------

---

| | | |
|:---|:---|:---|
| *Shares or*<br>*Principal Amounts* | *Shares or*<br>*Principal Amounts* | *Value* |
| Common Stocks– (continued) | Common Stocks– (continued) |  |
| Semiconductor & Semiconductor Equipment– (continued) | Semiconductor & Semiconductor Equipment– (continued) |  |
| Texas Instruments Inc | 94218 | $15566698 |
|  |  | 163305385 |
| Software – 7.0% | Software – 7.0% |  |
| Atlassian Corp - Class A\* | 28215 | 3630706 |
| Autodesk Inc\* | 47428 | 8862870 |
| Constellation Software Inc/Canada | 2960 | 4622043 |
| Microsoft Corp | 526086 | 126165945 |
| ServiceNow Inc\* | 16655 | 6466637 |
| Synopsys Inc\* | 69492 | 22188101 |
| Workday Inc - Class A\* | 116352 | 19469180 |
|  |  | 191405482 |
| Specialty Retail – 1.2% | Specialty Retail – 1.2% |  |
| O'Reilly Automotive Inc\* | 39061 | 32968656 |
| Technology Hardware, Storage & Peripherals – 3.9% | Technology Hardware, Storage & Peripherals – 3.9% |  |
| Apple Inc | 821965 | 106797912 |
| Textiles, Apparel & Luxury Goods – 2.1% | Textiles, Apparel & Luxury Goods – 2.1% |  |
| LVMH Moet Hennessy Louis Vuitton SE | 28219 | 20496631 |
| Moncler SpA | 199869 | 10637647 |
| NIKE Inc - Class B | 214830 | 25137258 |
|  |  | 56271536 |
| Trading Companies & Distributors – 2.2% | Trading Companies & Distributors – 2.2% |  |
| Ferguson PLC | 483760 | 61106750 |
| Wireless Telecommunication Services – 1.9% | Wireless Telecommunication Services – 1.9% |  |
| T-Mobile US Inc\* | 376545 | 52716300 |
| Total Common Stocks (cost $2,026,754,086) | Total Common Stocks (cost $2,026,754,086) | 2689226430 |
| Preferred Stocks– 0.8% | Preferred Stocks– 0.8% |  |
| Automobiles – 0.8% | Automobiles – 0.8% |  |
| Dr Ing hc F Porsche AG((cost $17,624,108) | 219364 | 22252676 |
| Private Placements– 0.2% | Private Placements– 0.2% |  |
| Health Care Providers & Services – 0.2% | Health Care Providers & Services – 0.2% |  |
| API Holdings Private Ltd\*<sup>,¢,§</sup>((cost $9,401,252) | 12941830 | 4746980 |
| Investments Purchased with Cash Collateral from Securities Lending– 0.1% | Investments Purchased with Cash Collateral from Securities Lending– 0.1% |  |
| Investment Companies – 0.1% | Investment Companies – 0.1% |  |
| Janus Henderson Cash Collateral Fund LLC, 4.1397%<sup>ºº,£</sup> | 968240 | 968240 |
| Time Deposits – 0% | Time Deposits – 0% |  |
| Royal Bank of Canada, 4.3100%, 1/3/23 | $242060 | 242060 |
| Total Investments Purchased with Cash Collateral from Securities Lending (cost $1,210,300) | Total Investments Purchased with Cash Collateral from Securities Lending (cost $1,210,300) | 1210300 |
| Total Investments (total cost $2,054,989,746) – 99.9% | Total Investments (total cost $2,054,989,746) – 99.9% | 2717436386 |
| Cash, Receivables and Other Assets, net of Liabilities – 0.1% | Cash, Receivables and Other Assets, net of Liabilities – 0.1% | 3874950 |
| Net Assets – 100% | Net Assets – 100% | $2721311336 |

---

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| | | |
|:---|:---|:---|
| ***Summary of Investments by Country - (Long Positions) (unaudited)*** | ***Summary of Investments by Country - (Long Positions) (unaudited)*** | ***Summary of Investments by Country - (Long Positions) (unaudited)*** |
|  |  | *% of* |
|  |  | *Investment* |
| *Country* | *Value* | *Securities* |
| United States | $1847478428 | 68.0% |
| France | 187543123 | 6.9 |
| United Kingdom | 170793524 | 6.3 |
| Canada | 123137744 | 4.5 |
| Netherlands | 110997085 | 4.1 |
| Sweden | 63086258 | 2.3 |
| Switzerland | 49474505 | 1.8 |
| Germany | 31537132 | 1.2 |
| Taiwan | 25085827 | 0.9 |
| Japan | 22942952 | 0.8 |
| Hong Kong | 20692228 | 0.8 |
| China | 19074710 | 0.7 |
| India | 16237624 | 0.6 |
| Ireland | 11177293 | 0.4 |
| Italy | 10637647 | 0.4 |
| Denmark | 7540306 | 0.3 |

---

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| | | | |
|:---|:---|:---|:---|
| Total | $2717436386 | 100.0 | % |

---

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#### Schedules of Affiliated Investments – (% of Net Assets)

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| | | | | |
|:---|:---|:---|:---|:---|
|  | *Dividend*<br>*Income* | *Realized*<br>*Gain/(Loss)* | *Change in*<br>*Unrealized*<br>*Appreciation/* <br>*Depreciation* | *Value*<br>*at 12/31/22* |
| Investment Companies - N/A | Investment Companies - N/A | Investment Companies - N/A | Investment Companies - N/A | Investment Companies - N/A |
| Money Markets - N/A | Money Markets - N/A | Money Markets - N/A | Money Markets - N/A | Money Markets - N/A |
| Janus Henderson Cash Liquidity Fund LLC, 4.2633%<sup>ºº</sup> | $14933 | $248 | $- | $- |
| Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% |
| Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% |
| Janus Henderson Cash Collateral Fund LLC, 4.1397%<sup>ºº</sup> | 9252<sup>∆</sup> | - | - | 968240 |
| Total Affiliated Investments - 0.1% | $24185 | $248 | $- | $968240 |

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| | | | | |
|:---|:---|:---|:---|:---|
|  | *Value*<br> *at 9/30/22* | *Purchases* | *Sales Proceeds* | *Value*<br>*at 12/31/22* |
| Investment Companies - N/A | Investment Companies - N/A | Investment Companies - N/A | Investment Companies - N/A | Investment Companies - N/A |
| Money Markets - N/A | Money Markets - N/A | Money Markets - N/A | Money Markets - N/A | Money Markets - N/A |
| Janus Henderson Cash Liquidity Fund LLC, 4.2633%<sup>ºº</sup> | 8553298 | 36272763 | (44826309) | - |
| Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% | Investments Purchased with Cash Collateral from Securities Lending - 0.1% |
| Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% |
| Janus Henderson Cash Collateral Fund LLC, 4.1397%<sup>ºº</sup> | 977540 | 41376704 | (41386004) | 968240 |

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#### Notes to Schedule of Investments (unaudited)

---

| | |
|:---|:---|
| ADR | American Depositary Receipt |
| LLC | Limited Liability Company |
| PLC | Public Limited Company |

---

<br> \* Non-income producing security.

<br> ºº Rate shown is the 7-day yield as of December 31, 2022.

<br> # Loaned security; a portion of the security is on loan at December 31, 2022.

---

| | |
|:---|:---|
| ¢ | Security is valued using significant unobservable inputs. The total value of Level 3 securities as of the period ended December 31, 2022 is $4,746,980, which represents 0.2% of net assets. |

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£The Fund may invest in certain securities that are considered affiliated companies. As defined by the Investment Company Act of 1940, as amended, an affiliated company is one in which the Fund owns 5% or more of the outstanding voting securities, or a company which is under common ownership or control.<br>

∆ Net of income paid
to the securities lending agent and rebates paid to the borrowing counterparties.

---

| | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|
| § | Schedule of Restricted Securities (as of December 31, 2022) | Schedule of Restricted Securities (as of December 31, 2022) | Schedule of Restricted Securities (as of December 31, 2022) | Schedule of Restricted Securities (as of December 31, 2022) | Schedule of Restricted Securities (as of December 31, 2022) | Schedule of Restricted Securities (as of December 31, 2022) |
|  |  |  |  |  | *Value as a* |  |
|  |  | *Acquisition* |  |  | *% of Net* |  |
|  |  | *Date* | *Cost* | *Value* | *Assets* |  |
| API Holdings Private Ltd | API Holdings Private Ltd | 9/27/21 | $9401252 | $4746980 | 0.2 | % |
| The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. | The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. | The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. | The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. | The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. | The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. | The Fund has registration rights for certain restricted securities held as of December 31, 2022. The issuer incurs all registration costs. |

---

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| | | | |
|:---|:---|:---|:---|
| The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. | The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial instruments as of December 31, 2022. |
| **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** |
|  |  | ***Level 2 -*** | ***Level 3 -*** |
|  | ***Level 1 -*** | ***Other Significant*** | ***Significant*** |
|  | ***Quoted Prices*** | ***Observable Inputs*** | ***Unobservable Inputs*** |
| **Assets** |  |  |  |
| **Investments In Securities:** |  |  |  |
| *Common Stocks* |  |  |  |
| Aerospace & Defense | $19298988 | $34854091 | $- |
| Banks | 110703955 | 55130879 | - |
| Beverages | 52415825 | 51600155 | - |
| Capital Markets | 97979072 | 8265461 | - |
| Electronic Equipment, Instruments & Components | - | 30282962 | - |
| Entertainment | 29135696 | 22942952 | - |
| Hotels, Restaurants & Leisure | 71616974 | 24222456 | - |
| Independent Power and Renewable Electricity Producers | 33573926 | 9284456 | - |
| Insurance | 54162735 | 28041926 | - |
| Interactive Media & Services | 73164540 | 6027160 | - |
| Internet & Direct Marketing Retail | 55247220 | 13047550 | - |
| Machinery | 64965676 | 57319168 | - |
| Metals & Mining | 52736126 | 19583503 | - |
| Oil, Gas & Consumable Fuels | 160546908 | 14287853 | - |
| Personal Products | - | 50939414 | - |
| Pharmaceuticals | 58726956 | 111521655 | - |
| Semiconductor & Semiconductor Equipment | 78161887 | 85143498 | - |
| Textiles, Apparel & Luxury Goods | 25137258 | 31134278 | - |
| Trading Companies & Distributors | - | 61106750 | - |
| All Other | 936916521 | - | - |
| *Preferred Stocks* | - | 22252676 | - |
| *Private Placements* | - | - | 4746980 |
| *Investments Purchased with Cash Collateral from Securities Lending* | - | 1210300 | - |
| **Total Assets** | $1974490263 | $738199143 | $4746980 |

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#### Investment Valuation
Fund holdings are valued in accordance with policies and procedures established by the Adviser pursuant to Rule 2a-5 under the 1940 Act and approved by and subject to the oversight of the Trustees (the "Valuation Procedures"). Equity securities traded on a domestic securities exchange are generally valued at readily available market quotations, which are (i) the official close prices or (ii) last sale prices on the primary market or exchange in which the securities trade. If such price is lacking for the trading period immediately preceding the time of determination, such securities are generally valued at their current bid price. Equity securities that are traded on a foreign exchange are generally valued at the closing prices on such markets. In the event that there is no current trading volume on a particular security in such foreign exchange, the bid price from the primary exchange is generally used to value the security. Foreign securities and currencies are converted to U.S. dollars using the current spot USD dollar exchange rate in effect at the close of the New York Stock Exchange ("NYSE"). The Fund will determine the market value of individual securities held by it by using prices provided by one or more approved professional pricing services or, as needed, by obtaining market quotations from independent broker-dealers. Most debt securities are valued in accordance with the evaluated bid price supplied by the Adviser-approved pricing service that is intended to reflect market value. The evaluated bid price supplied by the pricing service is an evaluation that may consider factors such as security prices, yields, maturities and ratings. Certain short-term securities maturing within 60 days or less may be evaluated and valued on an amortized cost basis provided that the amortized cost determined approximates market value. Securities for which market quotations or evaluated prices are not readily available or deemed unreliable are valued at fair value determined in good faith by the Adviser pursuant to the Valuation Procedures. Circumstances in which fair valuation may be utilized include, but are not limited to: (i) a significant event that may affect the securities of a single issuer, such as a merger, bankruptcy, or significant issuer-specific development; (ii) an event that may affect an entire market, such as a natural disaster or significant governmental action; (iii) a nonsignificant event such as a market closing early or not opening, or a security trading halt; and (iv) pricing of a nonvalued security and a restricted or nonpublic security. Special valuation considerations may apply with respect to "odd-lot" fixed-income transactions which, due to their small size, may receive evaluated prices by pricing services which reflect a large block trade and not what actually could be obtained for the odd-lot position. The valuation policies provide for the use of systematic fair valuation models provided by independent pricing services to value foreign equity securities in order to adjust for stale pricing, which may occur between the close of certain foreign exchanges and the close of the NYSE. The value of the securities of other mutual funds held by a Fund, if any, will be calculated using the NAV of such mutual funds.

#### Valuation Inputs Summary
FASB ASC 820, Fair Value Measurements and Disclosures ("ASC 820"), defines fair value, establishes a framework for measuring fair value, and expands disclosure requirements regarding fair value measurements. This standard emphasizes that fair value is a market-based measurement that should be determined based on the assumptions that market participants would use in pricing an asset or liability and establishes a hierarchy that prioritizes inputs to valuation techniques used to measure fair value. These inputs are summarized into three broad levels:

Level 1 – Unadjusted quoted prices in active markets the Fund has the ability to access for identical assets or liabilities.

Level 2 – Observable inputs other than unadjusted quoted prices included in Level 1 that are observable for the asset or liability either directly or indirectly. These inputs may include quoted prices for the identical instrument on an inactive market, prices for similar instruments, interest rates, prepayment speeds, credit risk, yield curves, default rates and similar data.

Assets or liabilities categorized as Level 2 in the hierarchy generally include: debt securities fair valued in accordance with the evaluated bid or ask prices supplied by a pricing service; securities traded on OTC markets and listed securities for which no sales are reported that are fair valued at the latest bid price (or yield equivalent thereof) obtained from one or more dealers transacting in a market for such securities or by a pricing service approved by the Fund's Trustees; certain short-term debt securities with maturities of 60 days or less that are fair valued at amortized cost; and equity securities of foreign issuers whose fair value is determined by using systematic fair valuation models provided by independent third parties in order to adjust for stale pricing which may occur between the close of certain foreign exchanges and the close of the NYSE. Other securities that may be categorized as Level 2 in the hierarchy include, but are not limited to, preferred stocks, bank loans, swaps, investments in unregistered investment companies, options, and forward contracts.

Level 3 – Unobservable inputs for the asset or liability to the extent that relevant observable inputs are not available, representing the Fund's own assumptions about the assumptions that a market participant would use in valuing the asset or liability, and that would be based on the best information available.

There have been no significant changes in valuation techniques used in valuing any such positions held by the Fund since the beginning of the fiscal year.

The inputs or methodology used for fair valuing securities are not necessarily an indication of the risk associated with investing in those securities. The summary of inputs used as of December 31, 2022 to fair value the Fund's investments

------

in securities and other financial instruments is included in the "Valuation Inputs Summary" in the Notes to Schedule of Investments.

The Fund did not hold a significant amount of Level 3 securities as of December 31, 2022.

------

For additional information on the Fund, please refer to the Fund's most recent semiannual or annual shareholder report.

125-25-70230 03-23<br>

------

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 DETROIT STREET

  - **City:** DENVER

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 3033333863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Global Research Fund

- **b. EDGAR series identifier (if any):** S000010489

- **c. LEI of Series:** C0GC60LX4ZGTZPLZA692

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2023-09-30

- **b. Date as of which information is reported:** 2022-12-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $2723525310.00

**Total Liabilities:** $2218962.00

**Net Assets:** $2721306348.00

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000077732 | -5.15%               | 7.96%                | 8.12%                |
| Class ID C000077733 | -5.19%               | 7.89%                | 8.05%                |
| Class ID C000083534 | -5.12%               | 7.98%                | 8.15%                |
| Class ID C000077734 | -5.11%               | 7.99%                | 8.15%                |
| Class ID C000114882 | -5.12%               | 8.00%                | 8.16%                |
| Class ID C000077735 | -5.18%               | 7.93%                | 8.10%                |
| Class ID C000077736 | -5.17%               | 7.95%                | 8.11%                |
| Class ID C000028951 | -5.13%               | 7.97%                | 8.14%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $17904246.00             | $-167786770.00                             |
| Month 2  | $285976.00               | $212374686.00                              |
| Month 3  | $1343166.00              | $200463290.00                              |

**Designated Index Information**

- **Index Name:** MSCI WORLD - Daily

- **Index Identifier:** MSCI.MC.MSCI990100GRS

### Schedule of Portfolio Investments

| Name                                 | Title                                      | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| N/A                                  | Royal Bank of Canada                       | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |    242060 | PA      | $242060.00    | 0.01%             | 2023-01-03      | Fixed         | 4.31%                 | No            |                  2 | On Loan: No      |
| Abbott Laboratories                  | Abbott Laboratories                        | CUSIP: 002824100<br>LEI: HQD377W2YR662HK5JX27 | Long             | EC               | CORP              | US        |     95751 | NS      | $10512502.00  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc                           | AbbVie Inc                                 | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |    251343 | NS      | $40619542.00  | 1.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Advanced Micro Devices Inc           | Advanced Micro Devices Inc                 | CUSIP: 007903107<br>LEI: R2I72C950HOYXII45366 | Long             | EC               | CORP              | US        |    203414 | NS      | $13175125.00  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Alphabet Inc                         | Alphabet Inc - Class C                     | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |    824575 | NS      | $73164540.00  | 2.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Amazon.com Inc                       | Amazon.com Inc                             | CUSIP: 023135106<br>LEI: ZXTILKJKG63JELOEG630 | Long             | EC               | CORP              | US        |    657705 | NS      | $55247220.00  | 2.03%             |  |  |  | No            |                  1 | On Loan: No      |
| American Tower Corp                  | American Tower Corp                        | CUSIP: 03027X100<br>LEI: 5493006ORUSIL88JOE18 | Long             | EC               | CORP              | US        |    102742 | NS      | $21766920.00  | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Apollo Global Management Inc         | Apollo Global Management Inc               | CUSIP: 03769M106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    319949 | NS      | $20409547.00  | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc                            | Apple Inc                                  | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |    821965 | NS      | $106797912.00 | 3.92%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlassian Corp                       | Atlassian Corp - Class A                   | CUSIP: 049468101<br>LEI: 549300V7ZY5P02D2MY38 | Long             | EC               | CORP              | US        |     28215 | NS      | $3630706.00   | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Autodesk Inc                         | Autodesk Inc                               | CUSIP: 052769106<br>LEI: FRKKVKAIQEF3FCSTPG55 | Long             | EC               | CORP              | US        |     47428 | NS      | $8862870.00   | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Bank of America Corp                 | Bank of America Corp                       | CUSIP: 060505104<br>LEI: 9DJT3UXIJIZJI4WXO774 | Long             | EC               | CORP              | US        |    870378 | NS      | $28826919.00  | 1.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Rio Tinto PLC                        | Rio Tinto PLC                              | CUSIP: G75754104<br>LEI: 213800YOEO5OQ72G2R82 | Long             | EC               | CORP              | GB        |    279161 | NS      | $19583503.00  | 0.72%             |  |  |  | No            |                  2 | On Loan: No      |
| Blackstone Inc                       | Blackstone Group Inc                       | CUSIP: 09260D107<br>LEI: 5299004LW4QWGZUB8Y96 | Long             | EC               | CORP              | US        |    211568 | NS      | $15696230.00  | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                 | Booking Holdings Inc                       | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |     15319 | NS      | $30872074.00  | 1.13%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                      | AstraZeneca PLC                            | CUSIP: G0593M107<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |    325735 | NS      | $44197840.00  | 1.62%             |  |  |  | No            |                  2 | On Loan: No      |
| Boston Scientific Corp               | Boston Scientific Corp                     | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |    369139 | NS      | $17080062.00  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Capital One Financial Corp           | Capital One Financial Corp                 | CUSIP: 14040H105<br>LEI: ZUE8T73ROZOF6FLBAR73 | Long             | EC               | CORP              | US        |    177184 | NS      | $16471025.00  | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Catalent Inc                         | Catalent Inc                               | CUSIP: 148806102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    121604 | NS      | $5473396.00   | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Centene Corp                         | Centene Corp                               | CUSIP: 15135B101<br>LEI: 549300Z7JJ4TQSQGT333 | Long             | EC               | CORP              | US        |    225420 | NS      | $18486694.00  | 0.68%             |  |  |  | No            |                  1 | On Loan: No      |
| Cheniere Energy Inc                  | Cheniere Energy Inc                        | CUSIP: 16411R208<br>LEI: MIHC87W9WTYSYZWV1J40 | Long             | EC               | CORP              | US        |     74560 | NS      | $11181018.00  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| ConocoPhillips                       | ConocoPhillips                             | CUSIP: 20825C104<br>LEI: WPTL2Z3FIYTHSP5V2253 | Long             | EC               | CORP              | US        |    284192 | NS      | $33534656.00  | 1.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Brands Inc             | Constellation Brands Inc                   | CUSIP: 21036P108<br>LEI: 5493005GKEG8QCVY7037 | Long             | EC               | CORP              | US        |    226174 | NS      | $52415825.00  | 1.93%             |  |  |  | No            |                  1 | On Loan: No      |
| Canadian Natural Resources Ltd       | Canadian Natural Resources Ltd             | CUSIP: 136385101<br>LEI: 549300T15RIMIKZC5Q38 | Long             | EC               | CORP              | CA        |    582543 | NS      | $32354416.00  | 1.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corp                         | Danaher Corp                               | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |     48983 | NS      | $13001068.00  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Deere & Co                           | Deere & Co                                 | CUSIP: 244199105<br>LEI: PWFTNG3EI0Y73OXWDH08 | Long             | EC               | CORP              | US        |     86332 | NS      | $37015708.00  | 1.36%             |  |  |  | No            |                  1 | On Loan: No      |
| DENTSPLY SIRONA Inc                  | DENTSPLY SIRONA Inc                        | CUSIP: 24906P109<br>LEI: 9NHEHR1SOZCYXDO8HO28 | Long             | EC               | CORP              | US        |    197822 | NS      | $6298652.00   | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| EOG Resources Inc                    | EOG Resources Inc                          | CUSIP: 26875P101<br>LEI: XWTZDRYZPBUHIQBKDB46 | Long             | EC               | CORP              | US        |    205646 | NS      | $26635270.00  | 0.98%             |  |  |  | No            |                  1 | On Loan: No      |
| Edwards Lifesciences Corp            | Edwards Lifesciences Corp                  | CUSIP: 28176E108<br>LEI: YA13X31F3V31L8TMPR58 | Long             | EC               | CORP              | US        |    102490 | NS      | $7646779.00   | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Teck Resources Ltd                   | Teck Resources Ltd                         | CUSIP: 878742204<br>LEI: T8KI5SQ2JRWHL7XY0E11 | Long             | EC               | CORP              | CA        |    837138 | NS      | $31641566.00  | 1.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Toronto-Dominion Bank/The            | The Toronto-Dominion Bank COM NPV          | CUSIP: 891160509<br>LEI: PT3QB789TSUIDF371261 | Long             | EC               | CORP              | CA        |    346728 | NS      | $22453571.00  | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Fidelity National Information        | Fidelity National Information Services Inc | CUSIP: 31620M106<br>LEI: 6WQI0GK1PRFVBA061U48 | Long             | EC               | CORP              | US        |    156364 | NS      | $10609297.00  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Freeport-McMoRan Inc                 | FREEPORT-MCMORAN INC                       | CUSIP: 35671D857<br>LEI: 549300IRDTHJQ1PVET45 | Long             | EC               | CORP              | US        |    555120 | NS      | $21094560.00  | 0.78%             |  |  |  | No            |                  1 | On Loan: No      |
| General Dynamics Corp                | General Dynamics Corp                      | CUSIP: 369550108<br>LEI: 9C1X8XOOTYY2FNYTVH06 | Long             | EC               | CORP              | US        |     77784 | NS      | $19298988.00  | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Global Payments Inc                  | Global Payments Inc                        | CUSIP: 37940X102<br>LEI: 549300NOMHGVQBX6S778 | Long             | EC               | CORP              | US        |    110897 | NS      | $11014290.00  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Airbus SE                            | Airbus SE                                  | CUSIP: N0280G100<br>LEI: MINO79WLOO247M1IL051 | Long             | EC               | CORP              | FR        |    293174 | NS      | $34854091.00  | 1.28%             |  |  |  | No            |                  2 | On Loan: No      |
| LVMH Moet Hennessy Louis Vuitt       | LVMH Moet Hennessy Louis Vuitton SE        | CUSIP: F58485115<br>LEI: IOG4E947OATN0KJYSD45 | Long             | EC               | CORP              | FR        |     28219 | NS      | $20496631.00  | 0.75%             |  |  |  | No            |                  2 | On Loan: No      |
| Humana Inc                           | HUMANA INC                                 | CUSIP: 444859102<br>LEI: 529900YLDW34GJAO4J06 | Long             | EC               | CORP              | US        |     42265 | NS      | $21647710.00  | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| JPMorgan Chase & Co                  | JPMorgan Chase & Co                        | CUSIP: 46625H100<br>LEI: 8I5DZWZKVSZI1NUHU748 | Long             | EC               | CORP              | US        |    443128 | NS      | $59423465.00  | 2.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Pernod Ricard SA                     | Pernod Ricard SA                           | CUSIP: F72027109<br>LEI: 52990097YFPX9J0H5D87 | Long             | EC               | CORP              | FR        |    262554 | NS      | $51600155.00  | 1.90%             |  |  |  | No            |                  2 | On Loan: No      |
| RWE AG                               | RWE AG                                     | CUSIP: D6629K109<br>LEI: 529900GB7KCA94ACC940 | Long             | EC               | CORP              | DE        |    208825 | NS      | $9284456.00   | 0.34%             |  |  |  | No            |                  2 | On Loan: No      |
| LPL Financial Holdings Inc           | LPL Financial Holdings Inc                 | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |     83707 | NS      | $18094942.00  | 0.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corp                    | LAM RESEARCH CORP                          | CUSIP: 512807108<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |     28434 | NS      | $11950810.00  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp-Liberty For       | Liberty Media Corp-Liberty Formula One     | CUSIP: 531229854<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |    487382 | NS      | $29135696.00  | 1.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co                       | Eli Lilly & Co                             | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |     35956 | NS      | $13154143.00  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Marathon Petroleum Corp              | Marathon Petroleum Corp                    | CUSIP: 56585A102<br>LEI: 3BNYRYQHD39K4LCKQF12 | Long             | EC               | CORP              | US        |    288737 | NS      | $33606099.00  | 1.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Sanofi                               | Sanofi                                     | CUSIP: F5548N101<br>LEI: 549300E9PC51EN656011 | Long             | EC               | CORP              | FR        |    184495 | NS      | $17849310.00  | 0.66%             |  |  |  | No            |                  2 | On Loan: No      |
| Marvell Technology Inc               | Marvell Technology Inc                     | CUSIP: 573874104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    291301 | NS      | $10789789.00  | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Mastercard Inc                       | Mastercard Inc                             | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |    138723 | NS      | $48238149.00  | 1.77%             |  |  |  | No            |                  1 | On Loan: No      |
| McDonald's Corp                      | McDonald's Corp                            | CUSIP: 580135101<br>LEI: UE2136O97NLB5BYP9H04 | Long             | EC               | CORP              | US        |    154612 | NS      | $40744900.00  | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Merck & Co Inc                       | Merck & Co Inc                             | CUSIP: 58933Y105<br>LEI: 4YV9Y5M8S0BRK1RP0397 | Long             | EC               | CORP              | US        |    299819 | NS      | $33264918.00  | 1.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp                       | MICROSOFT CORP                             | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |    526086 | NS      | $126165945.00 | 4.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Morgan Stanley                       | Morgan Stanley                             | CUSIP: 617446448<br>LEI: IGJSJL3JD5P30I6NJZ34 | Long             | EC               | CORP              | US        |    291700 | NS      | $24800334.00  | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| NextEra Energy Inc                   | NextEra Energy Inc                         | CUSIP: 65339F101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    165242 | NS      | $13814231.00  | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| NIKE Inc                             | NIKE Inc - Class B                         | CUSIP: 654106103<br>LEI: 787RXPR0UX0O0XUXPZ81 | Long             | EC               | CORP              | US        |    214830 | NS      | $25137258.00  | 0.92%             |  |  |  | No            |                  1 | On Loan: No      |
| Nintendo Co Ltd                      | NINTENDO CO LTD                            | CUSIP: J51699106<br>LEI: 353800FEEXU6I9M0ZF27 | Long             | EC               | CORP              | JP        |    547800 | NS      | $22942952.00  | 0.84%             |  |  |  | No            |                  2 | On Loan: No      |
| NVIDIA Corp                          | NVIDIA Corp                                | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |    182561 | NS      | $26679465.00  | 0.98%             |  |  |  | No            |                  1 | On Loan: No      |
| O'Reilly Automotive Inc              | O'Reilly Automotive Inc                    | CUSIP: 67103H107<br>LEI: 549300K2RLMQL149Q332 | Long             | EC               | CORP              | US        |     39061 | NS      | $32968656.00  | 1.21%             |  |  |  | No            |                  1 | On Loan: No      |
| OneMain Holdings Inc                 | OneMain Holdings Inc                       | CUSIP: 68268W103<br>LEI: 549300E3VEW8SMS5XW09 | Long             | EC               | CORP              | US        |    235056 | NS      | $7829715.00   | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Organon & Co                         | Organon & Co COMMON STOCK                  | CUSIP: 68622V106<br>LEI: 549300AMCKY57OK2CO56 | Long             | EC               | CORP              | US        |    244701 | NS      | $6834499.00   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| TSMC                                 | Taiwan Semiconductor Manufacturing Co Ltd  | CUSIP: Y84629107<br>LEI: 549300KB6NK5SBD14S87 | Long             | EC               | CORP              | TW        |   1721000 | NS      | $25085827.00  | 0.92%             |  |  |  | No            |                  2 | On Loan: No      |
| Parker-Hannifin Corp                 | Parker-Hannifin Corp                       | CUSIP: 701094104<br>LEI: 5493002CONDB4N2HKI23 | Long             | EC               | CORP              | US        |     96048 | NS      | $27949968.00  | 1.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Novartis AG                          | Novartis AG                                | CUSIP: H5820Q150<br>LEI: 5493007HIVTX6SY6XD66 | Long             | EC               | CORP              | CH        |    300287 | NS      | $27207747.00  | 1.00%             |  |  |  | No            |                  2 | On Loan: No      |
| Roche Holding AG                     | Roche Holding AG                           | CUSIP: H69293217<br>LEI: 549300U41AUUVOAAOB37 | Long             | EC               | CORP              | CH        |     70835 | NS      | $22266758.00  | 0.82%             |  |  |  | No            |                  2 | On Loan: No      |
| BNP Paribas SA                       | BNP Paribas SA                             | CUSIP: F1058Q238<br>LEI: R0MUWSFPU8MPRO8K5P83 | Long             | EC               | CORP              | FR        |    420730 | NS      | $23939211.00  | 0.88%             |  |  |  | No            |                  2 | On Loan: No      |
| Progressive Corp/The                 | Progressive Corp/The                       | CUSIP: 743315103<br>LEI: 529900TACNVLY9DCR586 | Long             | EC               | CORP              | US        |    205332 | NS      | $26633614.00  | 0.98%             |  |  |  | No            |                  1 | On Loan: No      |
| Sarepta Therapeutics Inc             | Sarepta Therapeutics Inc                   | CUSIP: 803607100<br>LEI: 549300IKDPIED8J8IG21 | Long             | EC               | CORP              | US        |    103442 | NS      | $13404014.00  | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Charles Schwab Corp/The              | Charles Schwab Corp                        | CUSIP: 808513105<br>LEI: 549300VSGCJ7E698NM85 | Long             | EC               | CORP              | US        |    340654 | NS      | $28362852.00  | 1.04%             |  |  |  | No            |                  1 | On Loan: No      |
| ServiceNow Inc                       | ServiceNow Inc                             | CUSIP: 81762P102<br>LEI: 549300HJTQM36M0E1G39 | Long             | EC               | CORP              | US        |     16655 | NS      | $6466637.00   | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| State Street Corp                    | STATE STREET CORP                          | CUSIP: 857477103<br>LEI: 549300ZFEEJ2IP5VME73 | Long             | EC               | CORP              | US        |    142126 | NS      | $11024714.00  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Synopsys Inc                         | Synopsys Inc                               | CUSIP: 871607107<br>LEI: QG7T915N9S0NY5UKNE63 | Long             | EC               | CORP              | US        |     69492 | NS      | $22188101.00  | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| T-Mobile US Inc                      | T-MOBILE US INC                            | CUSIP: 872590104<br>LEI: 549300QHIJYOHPACPG31 | Long             | EC               | CORP              | US        |    376545 | NS      | $52716300.00  | 1.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Tesla Inc                            | TESLA INC                                  | CUSIP: 88160R101<br>LEI: 54930043XZGB27CTOV49 | Long             | EC               | CORP              | US        |     83540 | NS      | $10290457.00  | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Texas Instruments Inc                | TEXAS INSTRUMENTS INC                      | CUSIP: 882508104<br>LEI: WDJNR2L6D8RWOEB8T652 | Long             | EC               | CORP              | US        |     94218 | NS      | $15566698.00  | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Thermo Fisher Scientific Inc         | THERMO FISHER SCIENTIFIC INC               | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |     41340 | NS      | $22765525.00  | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Janus Henderson Cash Collateral Fund | Janus Henderson Cash Collateral Fund       | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |    968240 | NS      | $968240.00    | 0.04%             |  |  |  | No            |                  2 | On Loan: No      |
| Uber Technologies Inc                | Uber Technologies Inc                      | CUSIP: 90353T100<br>LEI: 549300B2FTG34FILDR98 | Long             | EC               | CORP              | US        |    677577 | NS      | $16756479.00  | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| United Parcel Service Inc            | United Parcel Service Inc                  | CUSIP: 911312106<br>LEI: D01LMJZU09ULLNCY6Z23 | Long             | EC               | CORP              | US        |    259566 | NS      | $45122953.00  | 1.66%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Inc               | UnitedHealth Group Inc                     | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |     19534 | NS      | $10356536.00  | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Inc           | Vertex Pharmaceuticals Inc                 | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |     77891 | NS      | $22493363.00  | 0.83%             |  |  |  | No            |                  1 | On Loan: No      |
| Visa Inc                             | Visa Inc                                   | CUSIP: 92826C839<br>LEI: 549300JZ4OKEHW3DPJ59 | Long             | EC               | CORP              | US        |    212081 | NS      | $44061949.00  | 1.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Vistra Corp                          | Vistra Energy Corp                         | CUSIP: 92840M102<br>LEI: 549300KP43CPCUJOOG15 | Long             | EC               | CORP              | US        |   1447152 | NS      | $33573926.00  | 1.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Workday Inc                          | Workday Inc                                | CUSIP: 98138H101<br>LEI: 549300J0DYC0N31V7G13 | Long             | EC               | CORP              | US        |    116352 | NS      | $19469180.00  | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Intact Financial Corp                | INTACT FINANCIAL CORP                      | CUSIP: 45823T106<br>LEI: 5493009LXGE7Y4U9OY43 | Long             | EC               | CORP              | CA        |     61336 | NS      | $8830699.00   | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Alstom SA                            | Alstom SA                                  | CUSIP: F0259M475<br>LEI: 96950032TUYMW11FB530 | Long             | EC               | CORP              | FR        |    998656 | NS      | $24515872.00  | 0.90%             |  |  |  | No            |                  2 | On Loan: No      |
| London Stock Exchange Group PL       | London Stock Exchange Group PLC            | CUSIP: G5689U103<br>LEI: 213800QAUUUP6I445N30 | Long             | EC               | CORP              | GB        |     95814 | NS      | $8265461.00   | 0.30%             |  |  |  | No            |                  2 | On Loan: No      |
| Constellation Software Inc/Can       | Constellation Software Inc/Canada          | CUSIP: 21037X100<br>LEI: 549300B6PYHMCTDWQV29 | Long             | EC               | CORP              | CA        |      2960 | NS      | $4622043.00   | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| TotalEnergies SE                     | Total SE                                   | CUSIP: F92124100<br>LEI: 529900S21EQ1BO4ESM68 | Long             | EC               | CORP              | FR        |    228949 | NS      | $14287853.00  | 0.53%             |  |  |  | No            |                  2 | On Loan: No      |
| Suncor Energy Inc                    | Suncor Energy Inc                          | CUSIP: 867224107<br>LEI: 549300W70ZOQDVLCHY06 | Long             | EC               | CORP              | CA        |    732390 | NS      | $23235449.00  | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| AIA Group Ltd                        | AIA GROUP LTD                              | CUSIP: Y002A1105<br>LEI: ZP5ILWVSYE4LJGMMVD57 | Long             | EC               | CORP              | HK        |   1878100 | NS      | $20692228.00  | 0.76%             |  |  |  | No            |                  2 | On Loan: No      |
| Entain PLC                           | Entain PLC                                 | CUSIP: G427A6103<br>LEI: 213800GNI3K45LQR8L28 | Long             | EC               | CORP              | GB        |   1511045 | NS      | $24222456.00  | 0.89%             |  |  |  | No            |                  2 | On Loan: No      |
| ASML Holding NV                      | ASML HOLDING NV                            | CUSIP: N07059202<br>LEI: 724500Y6DUVHQD6OXN27 | Long             | EC               | CORP              | NL        |    112400 | NS      | $60833789.00  | 2.23%             |  |  |  | No            |                  2 | On Loan: No      |
| Moncler SpA                          | Moncler SpA                                | CUSIP: T6730E110<br>LEI: 815600EBD7FB00525B20 | Long             | EC               | CORP              | IT        |    199869 | NS      | $10637647.00  | 0.39%             |  |  |  | No            |                  2 | On Loan: No      |
| Ferguson PLC                         | Ferguson PLC                               | CUSIP: G3421J106<br>LEI: 213800DU1LGY3R2S2X42 | Long             | EC               | CORP              | GB        |    483760 | NS      | $61106750.00  | 2.25%             |  |  |  | No            |                  2 | On Loan: No      |
| HDFC Bank Ltd                        | HDFC Bank Ltd                              | CUSIP: Y3119P190<br>LEI: 335800ZQ6I4E2JXENC50 | Long             | EC               | CORP              | IN        |    583681 | NS      | $11490644.00  | 0.42%             |  |  |  | No            |                  2 | On Loan: No      |
| JD.com Inc                           | JD.Com Inc - Class A                       | CUSIP: G8208B101<br>LEI: 549300HVTWB0GJZ16V92 | Long             | EC               | CORP              | HK        |    464128 | NS      | $13047550.00  | 0.48%             |  |  |  | No            |                  2 | On Loan: No      |
| Atlas Copco AB                       | Atlas Copco AB - Class A                   | CUSIP: W1R924252<br>LEI: 213800T8PC8Q4FYJZR07 | Long             | EC               | CORP              | SE        |   2769172 | NS      | $32803296.00  | 1.21%             |  |  |  | No            |                  2 | On Loan: No      |
| Unilever PLC                         | Unilever PLC                               | CUSIP: G92087165<br>LEI: 549300MKFYEKVRWML317 | Long             | EC               | CORP              | NL        |   1015250 | NS      | $50939414.00  | 1.87%             |  |  |  | No            |                  2 | On Loan: No      |
| NatWest Group PLC                    | Natwest Group PLC                          | CUSIP: G6422B147<br>LEI: 2138005O9XJIJN4JPN90 | Long             | EC               | CORP              | GB        |   6173047 | NS      | $19701024.00  | 0.72%             |  |  |  | No            |                  2 | On Loan: No      |
| Tencent Holdings Ltd                 | TENCENT HOLDINGS LTD                       | CUSIP: G87572163<br>LEI: 254900N4SLUMW4XUYY11 | Long             | EC               | CORP              | HK        |    141800 | NS      | $6027160.00   | 0.22%             |  |  |  | No            |                  2 | On Loan: No      |
| Hexagon AB                           | Hexagon AB - Class B                       | CUSIP: W4R431112<br>LEI: 549300WJFW6ILNI4TA80 | Long             | EC               | CORP              | SE        |   2879277 | NS      | $30282962.00  | 1.11%             |  |  |  | No            |                  2 | On Loan: No      |
| Beazley PLC                          | Beazley PLC                                | CUSIP: G0936K107<br>LEI: 213800VTOMUWD41GIT12 | Long             | EC               | CORP              | GB        |    898163 | NS      | $7349698.00   | 0.27%             |  |  |  | No            |                  2 | On Loan: No      |
| Aon PLC                              | Aon PLC - Class A                          | CUSIP: G0403H108<br>LEI: 635400FALWQYX5E6QC64 | Long             | EC               | CORP              | US        |     62299 | NS      | $18698422.00  | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Horizon Therapeutics Plc             | Horizon Therapeutics Plc                   | CUSIP: G46188101<br>LEI: 529900P0GSQ79OOZEV53 | Long             | EC               | CORP              | US        |    141824 | NS      | $16139571.00  | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Linde PLC                            | Linde PLC                                  | CUSIP: G5494J103<br>LEI: 8945002PAZHZLBGKGF02 | Long             | EC               | CORP              | US        |    145544 | NS      | $47473542.00  | 1.75%             |  |  |  | No            |                  1 | On Loan: No      |
| API Holdings Ltd                     | API Holdings Private Ltd                   | CUSIP: N/A<br>LEI: 984500MB36F8114EB093       | Long             | EP               | CORP              | IN        |  12941830 | NS      | $4746980.00   | 0.17%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Dr Ing hc F Porsche AG               | Dr Ing hc F Porsche AG                     | CUSIP: D2R3HA114<br>LEI: 529900EWEX125AULXI58 | Long             | EP               | CORP              | DE        |    219364 | NS      | $22252676.00  | 0.82%             |  |  |  | No            |                  2 | On Loan: No      |
| Ascendis Pharma A/S                  | Ascendis Pharma A/S (ADR)                  | CUSIP: 04351P101<br>LEI: 549300B66JN3W3J8GH73 | Long             |  | CORP              | US        |     61740 | NS      | $7540306.00   | 0.28%             |  |  |  | No            |                  1 | On Loan: —       |
| Ryanair Holdings PLC                 | Ryanair Holdings PLC (ADR)                 | CUSIP: 783513203<br>LEI: 635400BR2ROC1FVEBQ56 | Long             |  | CORP              | US        |    149509 | NS      | $11177293.00  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2023-02-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER