# EDGAR Filing Document

**Accession Number:** 0001527753
**File Stem:** 0001805207-26-000003
**Filing Date:** 2026-6
**Character Count:** 35699
**Document Hash:** 603b3baed076c24e51bd18c1a8935ec6
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001805207-26-000003.hdr.sgml**: 20260610

**ACCESSION NUMBER**: 0001805207-26-000003

**CONFORMED SUBMISSION TYPE**: SCHEDULE 13D/A

**PUBLIC DOCUMENT COUNT**: 3

**FILED AS OF DATE**: 20260610

**DATE AS OF CHANGE**: 20260610

**SUBJECT COMPANY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Personalis, Inc.
- **CENTRAL INDEX KEY:** 0001527753
- **STANDARD INDUSTRIAL CLASSIFICATION:** SERVICES-MEDICAL LABORATORIES [8071]
- **ORGANIZATION NAME:** 08 Industrial Applications and Services
- **EIN:** 275411038
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** SCHEDULE 13D/A
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 005-91398
- **FILM NUMBER:** 261081149

**BUSINESS ADDRESS:**
- **STREET 1:** 6600 DUMBARTON CIRCLE
- **CITY:** FREMONT
- **STATE:** CA
- **ZIP:** 94555
- **BUSINESS PHONE:** 650-752-1300

**MAIL ADDRESS:**
- **STREET 1:** 6600 DUMBARTON CIRCLE
- **CITY:** FREMONT
- **STATE:** CA
- **ZIP:** 94555
**FILED BY**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Lightspeed Venture Partners Select IV, L.P.
- **CENTRAL INDEX KEY:** 0001805207

**ORGANIZATION NAME:**
- **EIN:** 000000000
- **STATE OF INCORPORATION:** E9

**FILING VALUES:**
- **FORM TYPE:** SCHEDULE 13D/A

**BUSINESS ADDRESS:**
- **STREET 1:** 2200 SAND HILL ROAD
- **CITY:** MENLO PARK
- **STATE:** CA
- **ZIP:** 94025
- **BUSINESS PHONE:** (650) 234-8300

**MAIL ADDRESS:**
- **STREET 1:** 2200 SAND HILL ROAD
- **CITY:** MENLO PARK
- **STATE:** CA
- **ZIP:** 94025

## Exhibit 99.1

EXHIBIT 99.1

JOINT FILING AGREEMENT

We, the undersigned, hereby express our agreement that the attached Schedule 13D (or any amendments thereto) relating to the common stock of Personalis, Inc. on behalf of each of us.

Dated: June 10, 2026

---

| | |
|:---|:---|
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br>**Lightspeed Venture Partners Select, L.P.**<br>By:<br> Lightspeed General Partner Select, L.P.<br> Its<br> General Partner<br>By:<br> Lightspeed Ultimate General Partner Select, Ltd.<br> Its<br> General Partner<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Director<br>**Lightspeed General Partner Select, L.P.**<br>By:<br> Lightspeed Ultimate General Partner Select, Ltd.<br> Its<br> General Partner<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Director<br>**Lightspeed Ultimate General Partner Select, Ltd.**<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Director<br>**Lightspeed Venture Partners VIII, L.P.**<br>By:<br> Lightspeed General Partner VIII, L.P.<br> Its<br> General Partner<br>By:<br> Lightspeed Ultimate General Partner VIII, Ltd.<br> Its<br> General Partner<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Director<br>**LSVP VIII Trust**<br>By: <br> Lightspeed Trustee VIII, LLC<br> its <br> Trustee<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Member | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<br>**Lightspeed Venture Partners Select IV, L.P.**<br>By:<br> Lightspeed General Partner Select IV, L.P.<br> Its<br> General Partner<br>By:<br> Lightspeed Ultimate General Partner Select IV, L.L.C.<br> Its<br> General Partner<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Managing Member<br>**Lightspeed General Partner Select IV, L.P.**<br>By:<br> Lightspeed Ultimate General Partner Select IV, L.L.C.<br> Its<br> General Partner<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Managing Member<br>**Lightspeed Ultimate General Partner Select IV, L.L.C.**<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Managing Member<br>**Lightspeed General Partner VIII, L.P.**<br>By:<br> Lightspeed Ultimate General Partner VIII, Ltd.<br> Its<br> General Partner<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Director<br>**Lightspeed Ultimate General Partner VIII, Ltd.**<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Director<br>**Lightspeed Trustee VIII, LLC**<br>By:<br> <u>/s/ Ravi Mhatre</u><br> Name: Ravi Mhatre<br> Title: Member |

---

## Exhibit 99.2

**EXHIBIT 99.2**

**SCHEDULE A** 

**GENERAL PARTNERS, CONTROL PERSONS, DIRECTORS AND EXECUTIVE OFFICERS OF CERTAIN REPORTING PERSONS**

---

| | | | |
|:---|:---|:---|:---|
| **Name** | **Business Address** | **Principal Occupation** | &nbsp;&nbsp;&nbsp;**Citizenship** |
| Barry Eggers | 2200 Sand Hill Road<br> Menlo Park, CA 94025<br>| Director of Lightspeed Ultimate General Partner VIII, Ltd. and Lightspeed Ultimate General Partner Select, Ltd.<br>| &nbsp;&nbsp;&nbsp;United States |
| Arif Janmohamed | 2200 Sand Hill Road<br> Menlo Park, CA 94025<br>| Managing Member of Lightspeed Ultimate General Partner Select IV, L.L.C. | &nbsp;&nbsp;&nbsp;United States |
| Ravi Mhatre | 2200 Sand Hill Road<br> Menlo Park, CA 94025 | Director of Lightspeed Ultimate General Partner VIII, Ltd. and Lightspeed Ultimate General Partner Select, Ltd.<br>Managing Member of Lightspeed Ultimate General Partner Select IV, L.L.C.<br>| &nbsp;&nbsp;&nbsp;United States |
| Peter Y. Nieh | 2200 Sand Hill Road<br> Menlo Park, CA 94025<br>| Director of Lightspeed Ultimate General Partner VIII, Ltd. and Lightspeed Ultimate General Partner Select, Ltd.<br>| &nbsp;&nbsp;&nbsp;United States |

---

### UNITED STATES SECURITIES AND EXCHANGE COMMISSION
**Washington, D.C. 20549**

## SCHEDULE 13D

### Under the Securities Exchange Act of 1934

**(Amendment No. 3)**

**Personalis, Inc.**

*(Name of Issuer)*

**Common Stock, par value $0.0001 per share**

*(Title of Class of Securities)*

**—**

*(CUSIP Number)*

**Brett Nissenberg**<br>c/o Lightspeed Venture Partners<br>2200 Sand Hill Road<br>Menlo Park CA 94025<br>650-234-8300

*(Name, Address and Telephone Number of Person Authorized to Receive Notices and Communications)*

**06/08/2026**

*(Date of Event Which Requires Filing of this Statement)*

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Venture Partners Select IV, L.P.** | Name of reporting person<br>**Lightspeed Venture Partners Select IV, L.P.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**WC** | Source of funds (See Instructions)<br>**WC** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2084240.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2084240.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2084240.00** | Aggregate amount beneficially owned by each reporting person<br>**2084240.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**PN** | Type of Reporting Person (See Instructions)<br>**PN** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed General Partner Select IV, L.P.** | Name of reporting person<br>**Lightspeed General Partner Select IV, L.P.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2084240.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2084240.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2084240.00** | Aggregate amount beneficially owned by each reporting person<br>**2084240.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**PN** | Type of Reporting Person (See Instructions)<br>**PN** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Ultimate General Partner Select IV, L.L.C.** | Name of reporting person<br>**Lightspeed Ultimate General Partner Select IV, L.L.C.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2084240.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2084240.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2084240.00** | Aggregate amount beneficially owned by each reporting person<br>**2084240.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Venture Partners Select, L.P.** | Name of reporting person<br>**Lightspeed Venture Partners Select, L.P.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**WC** | Source of funds (See Instructions)<br>**WC** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**1958726.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**1958726.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**1958726.00** | Aggregate amount beneficially owned by each reporting person<br>**1958726.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**1.9%** | Percent of class represented by amount in Row (11)<br>**1.9%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**PN** | Type of Reporting Person (See Instructions)<br>**PN** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed General Partner Select, L.P.** | Name of reporting person<br>**Lightspeed General Partner Select, L.P.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**1958726.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**1958726.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**1958726.00** | Aggregate amount beneficially owned by each reporting person<br>**1958726.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**1.9%** | Percent of class represented by amount in Row (11)<br>**1.9%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**PN** | Type of Reporting Person (See Instructions)<br>**PN** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Ultimate General Partner Select, Ltd.** | Name of reporting person<br>**Lightspeed Ultimate General Partner Select, Ltd.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**1958726.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**1958726.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**1958726.00** | Aggregate amount beneficially owned by each reporting person<br>**1958726.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**1.9%** | Percent of class represented by amount in Row (11)<br>**1.9%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Venture Partners VIII, L.P.** | Name of reporting person<br>**Lightspeed Venture Partners VIII, L.P.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**WC** | Source of funds (See Instructions)<br>**WC** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2058884.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2058884.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**PN** | Type of Reporting Person (See Instructions)<br>**PN** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed General Partner VIII, L.P.** | Name of reporting person<br>**Lightspeed General Partner VIII, L.P.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2058884.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2058884.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**PN** | Type of Reporting Person (See Instructions)<br>**PN** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Ultimate General Partner VIII, Ltd.** | Name of reporting person<br>**Lightspeed Ultimate General Partner VIII, Ltd.** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**CAYMAN ISLANDS** | Citizenship or place of organization<br>**CAYMAN ISLANDS** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2058884.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2058884.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**LSVP VIII Trust** | Name of reporting person<br>**LSVP VIII Trust** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**DELAWARE** | Citizenship or place of organization<br>**DELAWARE** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2058884.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2058884.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

| **CUSIP No.** | **—** |

---

| | | | |
|:--|:--|:--|:--|
| 1 | Name of reporting person<br>**Lightspeed Trustee VIII, LLC** | Name of reporting person<br>**Lightspeed Trustee VIII, LLC** | |
| 2 | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | Check the appropriate box if a member of a Group (See Instructions)<br>[ ] (a)<br>[x] (b) | |
| 3 | SEC use only | SEC use only | |
| 4 | Source of funds (See Instructions)<br>**AF** | Source of funds (See Instructions)<br>**AF** | |
| 5 | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)<br>[ ] | |
| 6 | Citizenship or place of organization<br>**DELAWARE** | Citizenship or place of organization<br>**DELAWARE** | |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 7 | Sole Voting Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 8 | Shared Voting Power<br>**2058884.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 9 | Sole Dispositive Power<br>**0.00** |
| Number of Shares<br>Beneficially Owned by<br>Each Reporting Person With: | 10 | Shared Dispositive Power<br>**2058884.00** |
| 11 | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | Aggregate amount beneficially owned by each reporting person<br>**2058884.00** | |
| 12 | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)<br>[ ] | |
| 13 | Percent of class represented by amount in Row (11)<br>**2.0%** | Percent of class represented by amount in Row (11)<br>**2.0%** | |
| 14 | Type of Reporting Person (See Instructions)<br>**OO** | Type of Reporting Person (See Instructions)<br>**OO** | |

---

**Item 1. Security and Issuer**

**(a) Title of Class of Securities:**
Common Stock, par value $0.0001 per share

**(b) Name of Issuer:**
Personalis, Inc.

**(c) Address of Issuer's Principal Executive Offices:**
6600 Dumbarton Circle, Fremont, CA, 94555

This Amendment No. 3 (this "Amendment No. 3" or this "Schedule 13D/A") amends and supplements the statement on Schedule 13D originally filed with the Securities and Exchange Commission (the "SEC") on August 24, 2020, and amended on March 7, 2022 and June 28, 2024 (as amended, the "Statement") by Lightspeed Venture Partners VIII, L.P. ("Lightspeed VIII"), Lightspeed General Partner VIII, L.P. ("LGP VIII"), Lightspeed Ultimate General Partner VIII, Ltd. ("LUGP VIII"), Lightspeed Venture Partners Select, L.P. ("Lightspeed Select"), Lightspeed General Partner Select, L.P. ("LGP Select"), Lightspeed Ultimate General Partner Select, Ltd. ("LUGP Select"), Lightspeed Venture Partners Select IV, L.P. ("Lightspeed Select IV"), Lightspeed General Partner Select IV, L.P. ("LGP Select IV"), Lightspeed Ultimate General Partner Select IV, L.L.C. ("LUGP Select IV"), LSVP VIII Trust ("Lightspeed VIII Trust"), Lightspeed Trustee VIII, LLC ("Lightspeed VIII Trustee"), Barry Eggers, Ravi Mhatre, Peter Y. Nieh and Arif Janmohamed. Unless otherwise defined herein, capitalized terms used in this Amendment No. 3 shall have the meanings ascribed to them in the Statement. Unless amended or supplemented below, the information in the Statement remains unchanged.

**Item 5. Interest in Securities of the Issuer**

**(a)**
Rows 11 and 13 of each Reporting Person's cover page to this Schedule 13D/A set forth the aggregate number of shares of common stock and percentages of the shares of common stock beneficially owned by such Reporting Person and are incorporated by reference. The percentage set forth in each row 13 is based upon 104,721,098 shares of common stock outstanding as of April 29, 2026, as reported in the Issuer's Quarterly Report on Form 10-Q filed with the SEC on May 7, 2026.

The Reporting Persons' beneficial ownership of the Issuer's securities includes (i) 2,084,240 shares of common stock directly held by Lightspeed Select IV; (ii) 1,958,726 shares of common stock directly held by Lightspeed Select; and (iii) 2,058,884 shares of common stock directly held by Lightspeed VIII. Collectively, the Reporting Persons beneficially own an aggregate of 6,101,850 shares of common stock, which represents 5.8% of the Issuer's outstanding common stock.

LUGP Select IV serves as the sole general partner of LGP Select IV, which serves as the sole general partner of Lightspeed Select IV.

LUGP Select serves as the sole general partner of LGP Select, which serves as the sole general partner of Lightspeed Select.

LUGP VIII serves as the sole general partner of LGP VIII, which serves as the sole general partner of Lightspeed VIII. The securities held by Lightspeed VIII are deemed to be held on behalf of, and for the benefit of, Lightspeed VIII Trust. Lightspeed VIII Trustee is the trustee of Lightspeed VIII Trust.

**(b)**
Rows 7 through 10 of each Reporting Person's cover page to this Schedule 13D/A set forth the number of shares of common stock as to which such Reporting Person has the sole or shared power to vote or direct the vote and sole or shared power to dispose or to direct the disposition and are incorporated by reference.

**(c)**
Except as set forth below, none of the Reporting Persons has effected any transactions with respect to the securities of the Issuer during the past sixty days.

On June 8, 2026, LSVP VIII Trust made a pro rata in-kind distribution for no additional consideration of 2,058,884 shares of common stock held by Lightspeed VIII to the beneficiaries of LSVP VIII Trust, which consist of LGP VIII and the limited partners of Lightspeed VIII. Of the shares distributed by LSVP VIII Trust, LGP VIII received 551,521 shares and subsequently made a pro rata in-kind distribution for no additional consideration of such shares of common stock to its members.

### SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Venture Partners Select IV, L.P.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** See Note 1

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed General Partner Select IV, L.P.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Lightspeed Ultimate General Partner Select IV, L.L.C., its General Partner, By Ravi Mhatre, Managing Member

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Ultimate General Partner Select IV, L.L.C.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Ravi Mhatre, Managing Member

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Venture Partners Select, L.P.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** See Note 2

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed General Partner Select, L.P.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Lightspeed Ultimate General Partner Select, Ltd., its General Partner, By Ravi Mhatre, Director

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Ultimate General Partner Select, Ltd.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Ravi Mhatre, Director

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Venture Partners VIII, L.P.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** See Note 3

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed General Partner VIII, L.P.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Lightspeed Ultimate General Partner VIII, Ltd., its General Partner, By Ravi Mhatre, Director

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Ultimate General Partner VIII, Ltd.

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Ravi Mhatre, Director

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** LSVP VIII Trust

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Lightspeed Trustee VIII, LLC, its Trustee, By Ravi Mhatre, Member

**Date:** 06/10/2026

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

**Reporting Person:** Lightspeed Trustee VIII, LLC

**Signature:** /s/ Ravi Mhatre

**Name/Title:** By Ravi Mhatre, Member

**Date:** 06/10/2026