# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001145549-25-057302
**Filing Date:** 2025-8
**Character Count:** 50362
**Document Hash:** 191e38754ee37ae553bc81a883a2308f
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001145549-25-057302.hdr.sgml**: 20250829

**ACCESSION NUMBER**: 0001145549-25-057302

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 2

**CONFORMED PERIOD OF REPORT**: 20250630

**FILED AS OF DATE**: 20250829

**DATE AS OF CHANGE**: 20250829

**PERIOD START**: 20250930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 251279163

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Growth and Income Fund (Series ID: S000010467)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028923 | Class T      | JAGIX           |
| C000077659 | Class A      | JDNAX           |
| C000077660 | Class C      | JGICX           |
| C000077661 | Class I      | JGINX           |
| C000077662 | Class R      | JDNRX           |
| C000077663 | Class S      | JADGX           |
| C000083515 | Class D      | JNGIX           |
| C000114870 | Class N      | JDNNX           |

## Nport-Ex

**Janus Henderson Growth and Income Fund**

**Schedule of Investments (unaudited)**

**June 30, 2025**

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | |
|:---|:---|:---|
|  | *Shares* | *Value* |
| Common Stocks – 100.0% |  |  |
| Aerospace & Defense – 2.4% |  |  |
| &nbsp;&nbsp;&nbsp; General Electric Co | &nbsp;&nbsp; 384742 | &nbsp;&nbsp;&nbsp;&nbsp; $99028743 |
| &nbsp;&nbsp;&nbsp; Raytheon Technologies Corp | &nbsp;&nbsp; 575146 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 83982819 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 183011562 |
| Airlines – 0.8% |  |  |
| &nbsp;&nbsp;&nbsp; Delta Air Lines Inc | 1209602 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 59488226 |
| Banks – 3.6% |  |  |
| &nbsp;&nbsp;&nbsp; JPMorgan Chase & Co | &nbsp;&nbsp; 953255 | &nbsp;&nbsp;&nbsp;&nbsp; 276358157 |
| Beverages – 0.4% |  |  |
| &nbsp;&nbsp;&nbsp; Constellation Brands Inc - Class A | &nbsp;&nbsp; 187407 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 30487371 |
| Biotechnology – 1.5% |  |  |
| &nbsp;&nbsp;&nbsp; AbbVie Inc | &nbsp;&nbsp; 406569 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 75467338 |
| &nbsp;&nbsp;&nbsp; Amgen Inc | &nbsp;&nbsp; 161884 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 45199631 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 120666969 |
| Building Products – 1.2% |  |  |
| &nbsp;&nbsp;&nbsp; Trane Technologies PLC | &nbsp;&nbsp; 217341 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 95067127 |
| Capital Markets – 6.9% |  |  |
| &nbsp;&nbsp;&nbsp; Charles Schwab Corp | &nbsp;&nbsp; 813457 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 74219817 |
| &nbsp;&nbsp;&nbsp; CME Group Inc | &nbsp;&nbsp; 222922 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 61441762 |
| &nbsp;&nbsp;&nbsp; Goldman Sachs Group Inc | &nbsp;&nbsp; 185930 | &nbsp;&nbsp;&nbsp;&nbsp; 131591957 |
| &nbsp;&nbsp;&nbsp; Intercontinental Exchange Inc | &nbsp;&nbsp; 475030 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 87153754 |
| &nbsp;&nbsp;&nbsp; Moody's Corp | &nbsp;&nbsp;&nbsp;&nbsp; 82963 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 41613411 |
| &nbsp;&nbsp;&nbsp; Morgan Stanley | &nbsp;&nbsp; 980338 | &nbsp;&nbsp;&nbsp;&nbsp; 138090411 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 534111112 |
| Chemicals – 0.7% |  |  |
| &nbsp;&nbsp;&nbsp; Corteva Inc | &nbsp;&nbsp; 726159 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 54120630 |
| Communications Equipment – 0.4% |  |  |
| &nbsp;&nbsp;&nbsp; Motorola Solutions Inc | &nbsp;&nbsp;&nbsp;&nbsp; 81313 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 34188864 |
| Consumer Finance – 2.5% |  |  |
| &nbsp;&nbsp;&nbsp; American Express Co | &nbsp;&nbsp; 607265 | &nbsp;&nbsp;&nbsp;&nbsp; 193705390 |
| Diversified Financial Services – 3.3% |  |  |
| &nbsp;&nbsp;&nbsp; Visa Inc | &nbsp;&nbsp; 714024 | &nbsp;&nbsp;&nbsp;&nbsp; 253514221 |
| Diversified Telecommunication Services – 0.7% |  |  |
| &nbsp;&nbsp;&nbsp; Verizon Communications Inc | 1200400 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 51941308 |
| Electrical Equipment – 1.4% |  |  |
| &nbsp;&nbsp;&nbsp; Eaton Corp PLC | &nbsp;&nbsp; 306583 | &nbsp;&nbsp;&nbsp;&nbsp; 109447065 |
| Electronic Equipment, Instruments & Components – 1.7% |  |  |
| &nbsp;&nbsp;&nbsp; Amphenol Corp | 1335455 | &nbsp;&nbsp;&nbsp;&nbsp; 131876181 |
| Entertainment – 1.9% |  |  |
| &nbsp;&nbsp;&nbsp; Walt Disney Co/The | 1170213 | &nbsp;&nbsp;&nbsp;&nbsp; 145118114 |
| Food & Staples Retailing – 0.9% |  |  |
| &nbsp;&nbsp;&nbsp; Costco Wholesale Corp | &nbsp;&nbsp;&nbsp;&nbsp; 67881 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 67198117 |
| Health Care Equipment & Supplies – 2.5% |  |  |
| &nbsp;&nbsp;&nbsp; Abbott Laboratories | &nbsp;&nbsp; 444242 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 60421355 |
| &nbsp;&nbsp;&nbsp; Medtronic PLC | &nbsp;&nbsp; 812102 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 70790931 |
| &nbsp;&nbsp;&nbsp; Stryker Corp | &nbsp;&nbsp; 153697 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 60807144 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 192019430 |
| Health Care Providers & Services – 1.6% |  |  |
| &nbsp;&nbsp;&nbsp; HCA Healthcare Inc | &nbsp;&nbsp; 148089 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 56732896 |
| &nbsp;&nbsp;&nbsp; UnitedHealth Group Inc | &nbsp;&nbsp; 219619 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 68514539 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 125247435 |
| Hotels, Restaurants & Leisure – 4.1% |  |  |
| &nbsp;&nbsp;&nbsp; Booking Holdings Inc | &nbsp;&nbsp;&nbsp;&nbsp; 20268 | &nbsp;&nbsp;&nbsp;&nbsp; 117336316 |
| &nbsp;&nbsp;&nbsp; Marriott International Inc/MD - Class A | &nbsp;&nbsp; 311470 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 85096719 |
| &nbsp;&nbsp;&nbsp; Royal Caribbean Cruises Ltd | &nbsp;&nbsp; 370862 | &nbsp;&nbsp;&nbsp;&nbsp; 116131727 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 318564762 |
| Independent Power and Renewable Electricity Producers – 0.9% |  |  |
| &nbsp;&nbsp;&nbsp; Vistra Corp | &nbsp;&nbsp; 373389 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 72366522 |
| Industrial Real Estate Investment Trusts (REITs) – 0.7% |  |  |
| &nbsp;&nbsp;&nbsp; Prologis Inc | &nbsp;&nbsp; 543208 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 57102025 |
| Information Technology Services – 1.7% |  |  |
| &nbsp;&nbsp;&nbsp; Accenture PLC | &nbsp;&nbsp; 434085 | &nbsp;&nbsp;&nbsp;&nbsp; 129743666 |
| Insurance – 2.1% |  |  |
| &nbsp;&nbsp;&nbsp; Marsh & McLennan Cos Inc | &nbsp;&nbsp; 213790 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 46743046 |

---

------

---

| | | |
|:---|:---|:---|
|  | *Shares* | *Value* |
| Common Stocks – (continued) |  |  |
| Insurance – (continued) |  |  |
| &nbsp;&nbsp;&nbsp; Progressive Corp/The | &nbsp;&nbsp; 248202 | &nbsp;&nbsp;&nbsp;&nbsp; $66235186 |
| &nbsp;&nbsp;&nbsp; Travelers Cos Inc | &nbsp;&nbsp; 178960 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 47878958 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 160857190 |
| Interactive Media & Services – 7.9% |  |  |
| &nbsp;&nbsp;&nbsp; Alphabet Inc - Class C | 1814286 | &nbsp;&nbsp;&nbsp;&nbsp; 321836193 |
| &nbsp;&nbsp;&nbsp; Meta Platforms Inc - Class A | &nbsp;&nbsp; 399976 | &nbsp;&nbsp;&nbsp;&nbsp; 295218286 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 617054479 |
| Life Sciences Tools & Services – 1.1% |  |  |
| &nbsp;&nbsp;&nbsp; Danaher Corp | &nbsp;&nbsp; 416833 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 82341191 |
| Machinery – 1.0% |  |  |
| &nbsp;&nbsp;&nbsp; Deere & Co | &nbsp;&nbsp; 159745 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 81228735 |
| Oil, Gas & Consumable Fuels – 1.5% |  |  |
| &nbsp;&nbsp;&nbsp; Chevron Corp | &nbsp;&nbsp; 515025 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 73746430 |
| &nbsp;&nbsp;&nbsp; ConocoPhillips | &nbsp;&nbsp; 516397 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 46341467 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 120087897 |
| Pharmaceuticals – 3.8% |  |  |
| &nbsp;&nbsp;&nbsp; Eli Lilly & Co | &nbsp;&nbsp; 188558 | &nbsp;&nbsp;&nbsp;&nbsp; 146986618 |
| &nbsp;&nbsp;&nbsp; Johnson & Johnson | &nbsp;&nbsp; 499034 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 76227444 |
| &nbsp;&nbsp;&nbsp; Zoetis Inc | &nbsp;&nbsp; 480714 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 74967348 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 298181410 |
| Professional Services – 1.9% |  |  |
| &nbsp;&nbsp;&nbsp; Automatic Data Processing Inc | &nbsp;&nbsp; 262185 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 80857854 |
| &nbsp;&nbsp;&nbsp; Booz Allen Hamilton Holding Corp | &nbsp;&nbsp; 622846 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 64856954 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 145714808 |
| Road & Rail – 0.8% |  |  |
| &nbsp;&nbsp;&nbsp; Union Pacific Corp | &nbsp;&nbsp; 255694 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 58830075 |
| Semiconductor & Semiconductor Equipment – 13.3% |  |  |
| &nbsp;&nbsp;&nbsp; Applied Materials Inc | &nbsp;&nbsp; 614649 | &nbsp;&nbsp;&nbsp;&nbsp; 112523792 |
| &nbsp;&nbsp;&nbsp; Broadcom Inc | 1421827 | &nbsp;&nbsp;&nbsp;&nbsp; 391926613 |
| &nbsp;&nbsp;&nbsp; KLA Corp | &nbsp;&nbsp; 225280 | &nbsp;&nbsp;&nbsp;&nbsp; 201792307 |
| &nbsp;&nbsp;&nbsp; Lam Research Corp | &nbsp;&nbsp; 859367 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 83650784 |
| &nbsp;&nbsp;&nbsp; Taiwan Semiconductor Manufacturing Co Ltd (ADR) | &nbsp;&nbsp; 601198 | &nbsp;&nbsp;&nbsp;&nbsp; 136165335 |
| &nbsp;&nbsp;&nbsp; Texas Instruments Inc | &nbsp;&nbsp; 501456 | &nbsp;&nbsp;&nbsp;&nbsp; 104112295 |
|  |  | &nbsp;&nbsp; 1030171126 |
| Software – 14.0% |  |  |
| &nbsp;&nbsp;&nbsp; Intuit Inc | &nbsp;&nbsp; 174537 | &nbsp;&nbsp;&nbsp;&nbsp; 137470578 |
| &nbsp;&nbsp;&nbsp; Microsoft Corp | 1421176 | &nbsp;&nbsp;&nbsp;&nbsp; 706907154 |
| &nbsp;&nbsp;&nbsp; Oracle Corp | &nbsp;&nbsp; 637787 | &nbsp;&nbsp;&nbsp;&nbsp; 139439372 |
| &nbsp;&nbsp;&nbsp; Salesforce Inc | &nbsp;&nbsp; 381370 | &nbsp;&nbsp;&nbsp;&nbsp; 103995785 |
|  |  | &nbsp;&nbsp; 1087812889 |
| Specialized Real Estate Investment Trusts (REITs) – 0.4% |  |  |
| &nbsp;&nbsp;&nbsp; Equinix Inc | &nbsp;&nbsp;&nbsp;&nbsp; 43975 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 34980793 |
| Specialty Retail – 2.2% |  |  |
| &nbsp;&nbsp;&nbsp; Home Depot Inc | &nbsp;&nbsp; 319141 | &nbsp;&nbsp;&nbsp;&nbsp; 117009856 |
| &nbsp;&nbsp;&nbsp; TJX Cos Inc | &nbsp;&nbsp; 445244 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 54983182 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 171993038 |
| Technology Hardware, Storage & Peripherals – 5.8% |  |  |
| &nbsp;&nbsp;&nbsp; Apple Inc | 1651068 | &nbsp;&nbsp;&nbsp;&nbsp; 338749621 |
| &nbsp;&nbsp;&nbsp; Dell Technologies Inc | &nbsp;&nbsp; 919911 | &nbsp;&nbsp;&nbsp;&nbsp; 112781089 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 451530710 |
| Textiles, Apparel & Luxury Goods – 0.9% |  |  |
| &nbsp;&nbsp;&nbsp; NIKE Inc - Class B | 1016642 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 72222248 |
| Tobacco – 1.5% |  |  |
| &nbsp;&nbsp;&nbsp; Philip Morris International Inc | &nbsp;&nbsp; 644447 | &nbsp;&nbsp;&nbsp;&nbsp; 117373132 |
| Total Common Stocks (cost $4,410,948,541) |  | &nbsp;&nbsp; 7765723975 |
| Investment Companies – 0.1% |  |  |
| Money Markets – 0.1% |  |  |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº,</sup><sup>£</sup> (cost $5,469,936) | 5468842 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 5469936 |
| Total Investments (total cost $4,416,418,477) – 100.1% |  | &nbsp;&nbsp; 7771193911 |
| Liabilities, net of Cash, Receivables and Other Assets – (0.1)% |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (6629707) |
| Net Assets – 100% |  | $7764564204 |

---

------

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | |
|:---|:---|:---|
| ***Summary of Investments by Country - (Long Positions) (unaudited)*** |  |  |
| *Country* | *Value* | &nbsp;&nbsp; *% of* <br>*Investment* <br>*Securities*<br>|
| United States | $7635028576 | &nbsp;&nbsp;&nbsp;&nbsp; 98.2<br> %<br>|
| Taiwan | 136165335 | &nbsp;&nbsp;&nbsp;&nbsp;1.8 |
| Total | $7771193911 | &nbsp;&nbsp;&nbsp;&nbsp; 100.0<br> %<br>|

---

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|:---|
| ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** |
| *Affiliate* | *Affiliated* <br>*Investments,* <br>*at Value at* <br>*9/30/24*<br>| *Purchases* | *Sales* <br>*Proceeds*<br>| *Realized* <br>*Gain/(Loss)*<br>| *Change in* <br>*Unrealized* <br>*Appreciation/* <br>*Depreciation*<br>| *Affiliated* <br>*Investments,* <br>*at Value at* <br>*6/30/25*<br>| *Ending* <br>*Shares*<br>| *Dividend* <br>*Income*<br>|
| Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% | Investment Companies - 0.1% |
| Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% | Money Markets - 0.1% |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.4188%<sup>ºº</sup>  |
|  | &nbsp;&nbsp; $5004446 | &nbsp;&nbsp; $228244445 | &nbsp;&nbsp; $(227778955) | &nbsp;&nbsp; $(410) | &nbsp;&nbsp; $410 | &nbsp;&nbsp; $5469936 | &nbsp;&nbsp; 5468842 | &nbsp;&nbsp; $210031 |

---

------

**Notes to Schedule of Investments (unaudited)** 

ADR American Depositary Receipt <br> LLC Limited Liability Company <br> PLC Public Limited Company

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| |
|:---|
| Rate shown is the 7-day yield as of June 30, 2025. |
| £&nbsp;&nbsp; The Fund may invest in certain securities that are considered affiliated companies. As defined by the Investment Company Act of 1940, as <br> amended, an affiliated company is one in which the Fund owns 5% or more of the outstanding voting securities, or a company which is <br> under common ownership or control.<br>|

---

The following is a summary of the inputs that were used to value the Fund's investments in securities and other financial

instruments as of June 30, 2025.

---

| | | | |
|:---|:---|:---|:---|
| **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** |
|  | ***Level 1 -*** <br>***Quoted Prices***<br>| &nbsp;&nbsp; ***Level 2 -*** <br>***Other Significant*** <br>***Observable Inputs***<br>| &nbsp;&nbsp; ***Level 3 -*** <br>***Significant*** <br>***Unobservable Inputs***<br>|
| **Assets** |  |  |  |
| **Investments In Securities:** |  |  |  |
| *Common Stocks* | $7765723975 | &nbsp;&nbsp;&nbsp; $- | &nbsp;&nbsp;&nbsp; $- |
| *Investment Companies* | - | &nbsp;&nbsp;&nbsp; 5469936 | &nbsp;&nbsp;&nbsp; - |
| **Total Assets** | $7765723975 | &nbsp;&nbsp;&nbsp; $5469936 | &nbsp;&nbsp;&nbsp; $- |

---

------

**Investment Valuation**

Fund holdings are valued in accordance with policies and procedures established by the Adviser pursuant to Rule 2a-5 under the 1940 Act and approved by and subject to the oversight of the Trustees (the "Valuation Procedures"). Equity securities traded on a domestic securities exchange are generally valued at readily available market quotations, which are (i) the official close prices or (ii) last sale prices on the primary market or exchange in which the securities trade. If such price is lacking for the trading period immediately preceding the time of determination, such securities are generally valued at their current bid price. Equity securities that are traded on a foreign exchange are generally valued at the closing prices on such markets. In the event that there is no current trading volume on a particular security in such foreign exchange, the bid price from the primary exchange is generally used to value the security. Foreign securities and currencies are converted to U.S. dollars using the current spot USD dollar exchange rate in effect at the close of the New York Stock Exchange ("NYSE"). The Adviser will determine the market value of individual securities held by it by using prices provided by one or more Adviser-approved professional pricing services or, as needed, by obtaining market quotations from independent broker-dealers. Most debt securities are valued in accordance with the evaluated bid price supplied by the pricing service that is intended to reflect market value. The evaluated bid price supplied by the pricing service is an evaluation that may consider factors such as security prices, yields, maturities, and ratings. Certain short-term securities maturing within 60 days or less may be evaluated and valued on an amortized cost basis provided that the amortized cost determined approximates market value. Securities for which market quotations or evaluated prices are not readily available or deemed unreliable are valued at fair value determined in good faith by the Adviser pursuant to the Valuation Procedures. Circumstances in which fair valuation may be utilized include, but are not limited to: (i) a significant event that may affect the securities of a single issuer, such as a merger, bankruptcy, or significant issuer-specific development; (ii) an event that may affect an entire market, such as a natural disaster or significant governmental action; (iii) a nonsignificant event such as a market closing early or not opening, or a security trading halt; and (iv) pricing of a non-valued security and a restricted or nonpublic security. Special valuation considerations may apply with respect to "odd-lot" fixed-income transactions which, due to their small size, may receive evaluated prices by pricing services which reflect a large block trade and not what actually could be obtained for the odd-lot position. The value of the securities of other mutual funds held by the Fund, if any, will be calculated using the NAV of such mutual funds, and the prospectuses for such mutual funds explain the circumstances under which they use fair valuation and the effects of using fair valuation. The value of the securities of any cash management pooled investment vehicles that

operate as money market funds held by the Fund, if any, will be calculated using the NAV of such funds.

**Valuation Inputs Summary**

FASB ASC 820, *Fair Value Measurements and Disclosures* ("ASC 820"), defines fair value, establishes a framework for measuring fair value, and expands disclosure requirements regarding fair value measurements. This standard emphasizes that fair value is a market-based measurement that should be determined based on the assumptions that market participants would use in pricing an asset or liability and establishes a hierarchy that prioritizes inputs to valuation techniques used to measure fair value. These inputs are summarized into three broad levels:

Level 1 – Unadjusted quoted prices in active markets the Fund has the ability to access for identical assets or liabilities.

Level 2 – Observable inputs other than unadjusted quoted prices included in Level 1 that are observable for the asset or liability either directly or indirectly. These inputs may include quoted prices for the identical instrument on an inactive market, prices for similar instruments, interest rates, prepayment speeds, credit risk, yield curves, default

rates and similar data.

Assets or liabilities categorized as Level 2 in the hierarchy generally include: debt securities fair valued in accordance with the evaluated bid or ask prices supplied by a pricing service; securities traded on OTC markets and listed securities for which no sales are reported that are fair valued at the latest bid price (or yield equivalent thereof) obtained from one or more dealers transacting in a market for such securities or by a pricing service approved by the Fund's Trustees; certain short-term debt securities with maturities of 60 days or less that are fair valued at amortized cost; and equity securities of foreign issuers whose fair value is determined by using systematic fair valuation models provided by independent third parties in order to adjust for stale pricing which may occur between the close of certain foreign exchanges and the close of the NYSE. Other securities that may be categorized as Level 2 in the hierarchy include, but are not limited to, preferred stocks, bank loans, swaps,

investments in unregistered investment companies, options, and forward contracts.

Level 3 – Unobservable inputs for the asset or liability to the extent that relevant observable inputs are not available, representing the Fund's own assumptions about the assumptions that a market participant would use in valuing the asset or liability, and that would be based on the best information available.

There have been no significant changes in valuation techniques used in valuing any such positions held by the Fund since the beginning of the fiscal period.

------

The inputs or methodology used for fair valuing securities are not necessarily an indication of the risk associated with investing in those securities. The summary of inputs used as of June 30, 2025 to fair value the Fund's investments in securities and other financial instruments is included in the "Valuation Inputs Summary" in the Notes to Schedule of Investments.

------

For additional information on the Fund, please refer to the Fund's most recent semiannual or annual financial

statements.

125-35-70241 08-25

------

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Growth and Income Fund

- **b. EDGAR series identifier (if any):** S000010467

- **c. LEI of Series:** 5Y0L1HFYUGXP9RNAEB69

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-09-30

- **b. Date as of which information is reported:** 2025-06-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $7769669132.47

**Total Liabilities:** $5104928.08

**Net Assets:** $7764564204.39

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028923 | 0.27%                | 7.73%                | 6.25%                |
| Class ID C000077659 | 0.27%                | 7.71%                | 6.24%                |
| Class ID C000077660 | 0.20%                | 7.67%                | 6.19%                |
| Class ID C000077661 | 0.29%                | 7.73%                | 6.27%                |
| Class ID C000077662 | 0.23%                | 7.67%                | 6.21%                |
| Class ID C000077663 | 0.26%                | 7.70%                | 6.23%                |
| Class ID C000083515 | 0.29%                | 7.72%                | 6.27%                |
| Class ID C000114870 | 0.30%                | 7.73%                | 6.27%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $2467564.00              | $10577483.00                               |
| Month 2  | $43844223.00             | $481407507.00                              |
| Month 3  | $8081192.00              | $445687362.00                              |

**Designated Index Information**

- **Index Name:** S&P 500

- **Index Identifier:** SP.MC.SPX

### Schedule of Portfolio Investments

| Name                                         | Title                                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| The TJX Companies Inc.                       | The TJX Companies Inc. COM USD1                                       | CUSIP: 872540109<br>LEI: V167QI9I69W364E2DY52 | Long             | EC               | CORP              | US        |    445440 | NS      | $55007385.60  | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Co., Ltd. | Taiwan Semiconductor Manufacturing Co., Ltd. SPON ADS REP 5 ORD TWD10 | CUSIP: 874039100<br>LEI: 549300KB6NK5SBD14S87 | Long             |  | CORP              | US        |    601462 | NS      | $136225128.38 | 1.75%             |  |  |  | No            |                  1 | On Loan: No      |
| Texas Instruments Incorporated               | Texas Instruments Incorporated COM USD1.00                            | CUSIP: 882508104<br>LEI: WDJNR2L6D8RWOEB8T652 | Long             | EC               | CORP              | US        |    501676 | NS      | $104157971.12 | 1.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Travelers Companies, Inc.                    | Travelers Companies, Inc. COM NPV                                     | CUSIP: 89417E109<br>LEI: 549300Y650407RU8B149 | Long             | EC               | CORP              | US        |    179039 | NS      | $47900094.06  | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| CME Group Inc.                               | CME Group Inc. COM USD0.01                                            | CUSIP: 12572Q105<br>LEI: LCZ7XYGSLJUHFXXNXD88 | Long             | EC               | CORP              | US        |    223020 | NS      | $61468772.40  | 0.79%             |  |  |  | No            |                  1 | On Loan: No      |
| Chevron Corp.                                | Chevron Corp. COM USD0.75                                             | CUSIP: 166764100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    515251 | NS      | $73778790.69  | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| ConocoPhillips                               | ConocoPhillips COM USD0.01                                            | CUSIP: 20825C104<br>LEI: WPTL2Z3FIYTHSP5V2253 | Long             | EC               | CORP              | US        |    516624 | NS      | $46361837.76  | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Brands, Inc.                   | Constellation Brands, Inc. COM USD0.01 CLASS A                        | CUSIP: 21036P108<br>LEI: 5493005GKEG8QCVY7037 | Long             | EC               | CORP              | US        |    187490 | NS      | $30500873.20  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Corteva Inc                                  | Corteva Inc COM USD0.01                                               | CUSIP: 22052L104<br>LEI: 549300WZN9I2QKLS0O94 | Long             | EC               | CORP              | US        |    726478 | NS      | $54144405.34  | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Costco Wholesale Corporation                 | Costco Wholesale Corporation COM USD0.01                              | CUSIP: 22160K105<br>LEI: 29DX7H14B9S6O3FD6V18 | Long             | EC               | CORP              | US        |     67910 | NS      | $67226825.40  | 0.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                          | Danaher Corporation COM USD0.01                                       | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |    417016 | NS      | $82377340.64  | 1.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Deere & Company                              | Deere & Company COM USD1                                              | CUSIP: 244199105<br>LEI: PWFTNG3EI0Y73OXWDH08 | Long             | EC               | CORP              | US        |    159815 | NS      | $81264329.35  | 1.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Dell Technologies Inc                        | Dell Technologies Inc COM USD0.01 CL C                                | CUSIP: 24703L202<br>LEI: 549300TJB5YBRUPOG437 | Long             | EC               | CORP              | US        |    920314 | NS      | $112830496.40 | 1.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Delta Air Lines Inc.                         | Delta Air Lines Inc. COM USD0.0001                                    | CUSIP: 247361702<br>LEI: Q2CCMS6R0AS67HJMBN42 | Long             | EC               | CORP              | US        |   1210132 | NS      | $59514291.76  | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                              | Eli Lilly & Co. COM NPV                                               | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |    188640 | NS      | $147050539.20 | 1.89%             |  |  |  | No            |                  1 | On Loan: No      |
| The Charles Schwab Corporation               | The Charles Schwab Corporation COM USD0.01                            | CUSIP: 808513105<br>LEI: 549300VSGCJ7E698NM85 | Long             | EC               | CORP              | US        |    813814 | NS      | $74252389.36  | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Abbott Laboratories                          | Abbott Laboratories COM NPV                                           | CUSIP: 002824100<br>LEI: HQD377W2YR662HK5JX27 | Long             | EC               | CORP              | US        |    444437 | NS      | $60447876.37  | 0.78%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                  | AbbVie Inc. COM USD0.01                                               | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |    406748 | NS      | $75500563.76  | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Alphabet Inc.                                | Alphabet Inc. CAP USD0.001 CL C                                       | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |   1815081 | NS      | $321977218.59 | 4.15%             |  |  |  | No            |                  1 | On Loan: No      |
| American Express Company                     | American Express Company COM USD0.20                                  | CUSIP: 025816109<br>LEI: R4PP93JZOLY261QX3811 | Long             | EC               | CORP              | US        |    607532 | NS      | $193790557.36 | 2.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                                   | Amgen Inc. COM USD0.0001                                              | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |    161955 | NS      | $45219455.55  | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Amphenol Corporation                         | Amphenol Corporation CL'A'COM USD0.001                                | CUSIP: 032095101<br>LEI: 549300D3L3G0R4U4VT04 | Long             | EC               | CORP              | US        |   1336039 | NS      | $131933851.25 | 1.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                                   | Apple Inc. COM USD0.00001                                             | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |   1651792 | NS      | $338898164.64 | 4.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Applied Materials Inc.                       | Applied Materials Inc. COM USD0.01                                    | CUSIP: 038222105<br>LEI: 41BNNE1AFPNAZELZ6K07 | Long             | EC               | CORP              | US        |    614919 | NS      | $112573221.33 | 1.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Automatic Data Processing, Inc.              | Automatic Data Processing, Inc. COM USD0.10                           | CUSIP: 053015103<br>LEI: HGBOLILQXWER4SAL2I23 | Long             | EC               | CORP              | US        |    262300 | NS      | $80893320.00  | 1.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                         | Booking Holdings Inc COM USD0.008                                     | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |     20276 | NS      | $117382630.24 | 1.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Booz Allen Hamilton Holding Corp.            | Booz Allen Hamilton Holding Corp. COM USD0.01 CLASS 'A'               | CUSIP: 099502106<br>LEI: 529900JPV47PIUWMA015 | Long             | EC               | CORP              | US        |    623119 | NS      | $64885381.47  | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadcom Inc                                 | Broadcom Inc COM USD0.001                                             | CUSIP: 11135F101<br>LEI: 549300WV6GIDOZJTV909 | Long             | EC               | CORP              | US        |   1422450 | NS      | $392098342.50 | 5.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Meta Platforms Inc                           | Meta Platforms Inc COM USD0.000006 CL 'A'                             | CUSIP: 30303M102<br>LEI: BQ4BKCS1HXDV9HN80Z93 | Long             | EC               | CORP              | US        |    400152 | NS      | $295348189.68 | 3.80%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                    | KLA Corp. COM USD0.001                                                | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    225378 | NS      | $201880089.72 | 2.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corporation                     | Lam Research Corporation COM NEW                                      | CUSIP: 512807306<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |    859744 | NS      | $83687480.96  | 1.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Marsh & McLennan Companies, Inc.             | Marsh & McLennan Companies, Inc. COM USD1.00                          | CUSIP: 571748102<br>LEI: 549300XMP3KDCKJXIU47 | Long             | EC               | CORP              | US        |    213884 | NS      | $46763597.76  | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Marriott International, Inc.                 | Marriott International, Inc. COM USD0.01 CLASS A                      | CUSIP: 571903202<br>LEI: 225YDZ14ZO8E1TXUSU86 | Long             | EC               | CORP              | US        |    311607 | NS      | $85134148.47  | 1.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp.                              | Microsoft Corp. COM USD0.00000625                                     | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |   1421799 | NS      | $707217040.59 | 9.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Moodys Corp.                                 | Moodys Corp. COM USD0.01                                              | CUSIP: 615369105<br>LEI: 549300GCEDD8YCF5WU84 | Long             | EC               | CORP              | US        |     83000 | NS      | $41631970.00  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Morgan Stanley                               | Morgan Stanley COM USD0.01                                            | CUSIP: 617446448<br>LEI: IGJSJL3JD5P30I6NJZ34 | Long             | EC               | CORP              | US        |    980768 | NS      | $138150980.48 | 1.78%             |  |  |  | No            |                  1 | On Loan: No      |
| Motorola Solutions Inc                       | Motorola Solutions Inc COM USD0.01                                    | CUSIP: 620076307<br>LEI: 6S552MUG6KGJVEBSEC55 | Long             | EC               | CORP              | US        |     81349 | NS      | $34204000.54  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Nike, Inc.                                   | Nike, Inc. CLASS'B'COM NPV                                            | CUSIP: 654106103<br>LEI: 787RXPR0UX0O0XUXPZ81 | Long             | EC               | CORP              | US        |   1017088 | NS      | $72253931.52  | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| Oracle Corp.                                 | Oracle Corp. COM USD0.01                                              | CUSIP: 68389X105<br>LEI: 1Z4GXXU7ZHVWFCD8TV52 | Long             | EC               | CORP              | US        |    638067 | NS      | $139500588.21 | 1.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Walt Disney Company                          | Walt Disney Company COM DISNEY USD0.01                                | CUSIP: 254687106<br>LEI: 549300GZKULIZ0WOW665 | Long             | EC               | CORP              | US        |   1170726 | NS      | $145181731.26 | 1.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Accenture PLC                                | Accenture PLC CLS'A'USD0.0000225                                      | CUSIP: G1151C101<br>LEI: 5493000EWHDSR3MZWH98 | Long             | EC               | CORP              | US        |    434276 | NS      | $129800753.64 | 1.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Medtronic PLC                                | Medtronic PLC USD0.0001                                               | CUSIP: G5960L103<br>LEI: 549300GX3ZBSQWUXY261 | Long             | EC               | CORP              | US        |    812458 | NS      | $70821963.86  | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Visa Inc                                     | Visa Inc COM STK USD0.0001                                            | CUSIP: 92826C839<br>LEI: 549300JZ4OKEHW3DPJ59 | Long             | EC               | CORP              | US        |    714337 | NS      | $253625351.85 | 3.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Vistra Corp                                  | Vistra Corp COM USD0.01                                               | CUSIP: 92840M102<br>LEI: 549300KP43CPCUJOOG15 | Long             | EC               | CORP              | US        |    373553 | NS      | $72398306.93  | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| Zoetis Inc.                                  | Zoetis Inc. COM USD0.01 CL 'A'                                        | CUSIP: 98978V103<br>LEI: 549300HD9Q1LOC9KLJ48 | Long             | EC               | CORP              | US        |    480925 | NS      | $75000253.75  | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Philip Morris International Inc.             | Philip Morris International Inc. COM NPV                              | CUSIP: 718172109<br>LEI: HL3H1H2BGXWVG3BSWR90 | Long             | EC               | CORP              | US        |    644730 | NS      | $117424674.90 | 1.51%             |  |  |  | No            |                  1 | On Loan: No      |
| The Progressive Corporation                  | The Progressive Corporation COM USD1.00                               | CUSIP: 743315103<br>LEI: 529900TACNVLY9DCR586 | Long             | EC               | CORP              | US        |    248311 | NS      | $66264273.46  | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Prologis Inc.                                | Prologis Inc. COM USD0.01                                             | CUSIP: 74340W103<br>LEI: 529900DFH19P073LZ636 | Long             | EC               | CORP              | US        |    543446 | NS      | $57127043.52  | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| RTX Corp.                                    | RTX Corp. COM                                                         | CUSIP: 75513E101<br>LEI: I07WOS4YJ0N7YRFE7309 | Long             | EC               | CORP              | US        |    575398 | NS      | $84019615.96  | 1.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Salesforce Inc.                              | Salesforce Inc. COM USD0.001                                          | CUSIP: 79466L302<br>LEI: RCGZFPDMRW58VJ54VR07 | Long             | EC               | CORP              | US        |    381538 | NS      | $104041597.22 | 1.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Stryker Corporation                          | Stryker Corporation COM USD0.10                                       | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |    153765 | NS      | $60834046.95  | 0.78%             |  |  |  | No            |                  1 | On Loan: No      |
| Union Pacific Corporation                    | Union Pacific Corporation COM USD2.50                                 | CUSIP: 907818108<br>LEI: 549300LMMRSZZCZ8CL11 | Long             | EC               | CORP              | US        |    255807 | NS      | $58856074.56  | 0.76%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Inc.                      | UnitedHealth Group Inc. COM USD0.01                                   | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |    219716 | NS      | $68544800.52  | 0.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Verizon Communications Inc.                  | Verizon Communications Inc. COM USD0.10                               | CUSIP: 92343V104<br>LEI: 2S72QS2UO2OESLG6Y829 | Long             | EC               | CORP              | US        |   1200926 | NS      | $51964068.02  | 0.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Trane Technologies PLC                       | Trane Technologies PLC COM USD1                                       | CUSIP: G8994E103<br>LEI: 549300BURLR9SLYY2705 | Long             | EC               | CORP              | US        |    217437 | NS      | $95109118.17  | 1.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Royal Caribbean Group                        | Royal Caribbean Group COM USD0.01                                     | CUSIP: V7780T103<br>LEI: K2NEH8QNVW44JIWK7Z55 | Long             | EC               | CORP              | US        |    371025 | NS      | $116182768.50 | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Equinix, Inc.                                | Equinix, Inc. COM USD0.001                                            | CUSIP: 29444U700<br>LEI: 549300EVUN2BTLJ3GT74 | Long             | EC               | CORP              | US        |     43994 | NS      | $34995907.18  | 0.45%             |  |  |  | No            |                  1 | On Loan: No      |
| GE Aerospace                                 | GE Aerospace COM NEW                                                  | CUSIP: 369604301<br>LEI: 3C7474T6CDKPR9K6YT90 | Long             | EC               | CORP              | US        |    384911 | NS      | $99072242.29  | 1.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Goldman Sachs Group, Inc.                    | Goldman Sachs Group, Inc. COM USD0.01                                 | CUSIP: 38141G104<br>LEI: 784F5XWPLTWKTBV3E584 | Long             | EC               | CORP              | US        |    186012 | NS      | $131649993.00 | 1.70%             |  |  |  | No            |                  1 | On Loan: No      |
| HCA Healthcare Inc                           | HCA Healthcare Inc COM USD0.01                                        | CUSIP: 40412C101<br>LEI: 529900PH4ZGUH2MNEU89 | Long             | EC               | CORP              | US        |    148154 | NS      | $56757797.40  | 0.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Home Depot Inc.                              | Home Depot Inc. COM USD0.05                                           | CUSIP: 437076102<br>LEI: QEKMOTMBBKA8I816DO57 | Long             | EC               | CORP              | US        |    319281 | NS      | $117061185.84 | 1.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Intercontinental Exchange Inc                | Intercontinental Exchange Inc COM USD0.01                             | CUSIP: 45866F104<br>LEI: 5493000F4ZO33MV32P92 | Long             | EC               | CORP              | US        |    475239 | NS      | $87192099.33  | 1.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuit Inc                                   | Intuit Inc COM USD0.01                                                | CUSIP: 461202103<br>LEI: VI90HBPH7XSFMB9E4M29 | Long             | EC               | CORP              | US        |    174613 | NS      | $137530437.19 | 1.77%             |  |  |  | No            |                  1 | On Loan: No      |
| JPMorgan Chase & Co.                         | JPMorgan Chase & Co. COM USD1.00                                      | CUSIP: 46625H100<br>LEI: 8I5DZWZKVSZI1NUHU748 | Long             | EC               | CORP              | US        |    953673 | NS      | $276479339.43 | 3.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Johnson & Johnson                            | Johnson & Johnson COM USD1.00                                         | CUSIP: 478160104<br>LEI: 549300G0CFPGEF6X2043 | Long             | EC               | CORP              | US        |    499253 | NS      | $76260895.75  | 0.98%             |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC                      | Janus Henderson Cash Liquidity Fund LLC                               | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |   5468842 | NS      | $5469936.06   | 0.07%             |  |  |  | No            |                  2 | On Loan: No      |
| Eaton Corporation PLC                        | Eaton Corporation PLC COM USD0.01                                     | CUSIP: G29183103<br>LEI: 549300VDIGTMXUNT7H71 | Long             | EC               | CORP              | US        |    306718 | NS      | $109495258.82 | 1.41%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2025-08-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER