# EDGAR Filing Document

**Accession Number:** 0001424958
**File Stem:** 0001145549-25-044653
**Filing Date:** 2025-6
**Character Count:** 52312
**Document Hash:** 8b63425dfcc0bdb985b90513c891d91c
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001145549-25-044653.hdr.sgml**: 20250627

**ACCESSION NUMBER**: 0001145549-25-044653

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250430

**FILED AS OF DATE**: 20250627

**DATE AS OF CHANGE**: 20250627

**PERIOD START**: 20251031

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Direxion Shares ETF Trust
- **CENTRAL INDEX KEY:** 0001424958

**ORGANIZATION NAME:**
- **EIN:** 000000000
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1031

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-22201
- **FILM NUMBER:** 251087500

**BUSINESS ADDRESS:**
- **STREET 1:** 1301 AVENUE OF THE AMERICAS (6TH AVENUE)
- **STREET 2:** 28TH FLOOR
- **CITY:** NEW YORK
- **STATE:** NY
- **ZIP:** 10019
- **BUSINESS PHONE:** 646-572-3390

**MAIL ADDRESS:**
- **STREET 1:** 1301 AVENUE OF THE AMERICAS (6TH AVENUE)
- **STREET 2:** 28TH FLOOR
- **CITY:** NEW YORK
- **STATE:** NY
- **ZIP:** 10019

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** Direxion ETF Trust
- **DATE OF NAME CHANGE:** 20080124

## Series and Classes Contracts Data

### Direxion Daily S&P Biotech Bull 3X Shares (Series ID: S000049373)

| Class ID   | Class Name                                | Ticker Symbol   |
|:---|:---|:---|
| C000156149 | Direxion Daily S&P Biotech Bull 3X Shares | LABU            |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** Direxion Shares ETF Trust

- **b. Investment Company Act file number:** 811-22201

- **c. CIK number of Registrant:** 0001424958

- **d. LEI of Registrant:** 549300M501IVJM50FG12

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 1301 Avenue of the Americas (6th Avenue)

  - **City:** New York

  - **State:** NY

  - **Foreign country:** US

  - **Zip / Postal Code:** 10019

  - **Telephone number:** 646-572-3390

**Item A.2. Information about the Series.**

- **a. Name of Series:** Direxion Daily S&P Biotech Bull 3X Shares

- **b. EDGAR series identifier (if any):** S000049373

- **c. LEI of Series:** 549300WNBLZ8IWC9IW36

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-10-31

- **b. Date as of which information is reported:** 2025-04-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $624619933.88

**Total Liabilities:** $21626554.60

**Net Assets:** $602993379.28

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000156149 | -14.01%              | -25.95%              | -0.30%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $-6463899.82             | $-15250926.76                              |
| Month 2  | $-20645503.14            | $-19768049.20                              |
| Month 3  | $-45509103.85            | $49210908.77                               |

### Schedule of Portfolio Investments

| Name                                | Title                                                               | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Adma Biologics Inc                  | Adma Biologics Inc COM USD0.0001                                    | CUSIP: 000899104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    202091 | NS      | $4809765.80   | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                         | AbbVie Inc. COM USD0.01                                             | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |     46118 | NS      | $8997621.80   | 1.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Acadia Pharmaceuticals Inc          | Acadia Pharmaceuticals Inc COM USD0.0001                            | CUSIP: 004225108<br>LEI: 529900O3044NO0PEA039 | Long             | EC               | CORP              | US        |    181230 | NS      | $2645958.00   | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Spyre Therapeutics Inc              | SPYRE THERAPEUTICS INC                                              | CUSIP: 00773J202<br>LEI: 52990017AIJPRH3HQH40 | Long             | EC               | CORP              | US        |     63626 | NS      | $969023.98    | 0.16%             |  |  |  | No            |                  1 | On Loan: —       |
| Agios Pharmaceuticals Inc           | Agios Pharmaceuticals Inc COM USD0.001                              | CUSIP: 00847X104<br>LEI: 529900ONJHY8EOGYT555 | Long             | EC               | CORP              | US        |     84720 | NS      | $2515336.80   | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Akebia Therapeutics Inc.            | AKEBIA THERAPEUTICS INC                                             | CUSIP: 00972D105<br>LEI: 549300CBQEHMKEEGNX42 | Long             | EC               | CORP              | US        |    186979 | NS      | $450619.39    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Akero Therapeutics Inc              | Akero Therapeutics Inc COM USD0.0001                                | CUSIP: 00973Y108<br>LEI: 549300SI3KG74LBE2955 | Long             | EC               | CORP              | US        |     67042 | NS      | $3057785.62   | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Alnylam Pharmaceuticals Inc.        | Alnylam Pharmaceuticals Inc. COM USD0.01                            | CUSIP: 02043Q107<br>LEI: 529900S3ZI14OWRJII50 | Long             | EC               | CORP              | US        |     40332 | NS      | $10616995.68  | 1.76%             |  |  |  | No            |                  1 | On Loan: No      |
| Altimmune Inc.                      | ALTIMMUNE INC                                                       | CUSIP: 02155H200<br>LEI: 549300EOYCQ8SW3NVE64 | Long             | EC               | CORP              | US        |    130009 | NS      | $682547.25    | 0.11%             |  |  |  | No            |                  1 | On Loan: —       |
| Amgen Inc.                          | Amgen Inc. COM USD0.0001                                            | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |     31296 | NS      | $9104632.32   | 1.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Amicus Therapeutics Inc.            | Amicus Therapeutics Inc. COM STK USD0.01                            | CUSIP: 03152W109<br>LEI: 549300MRD1SMUTNBPQ53 | Long             | EC               | CORP              | US        |    229620 | NS      | $1763481.60   | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| AnaptysBio Inc.                     | ANAPTYSBIO INC                                                      | CUSIP: 032724106<br>LEI: 529900HH4WQT03IG1A12 | Long             | EC               | CORP              | US        |     28201 | NS      | $626626.22    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Anavex Life Sciences Corp.          | Anavex Life Sciences Corp. USD0.001                                 | CUSIP: 032797300<br>LEI: 549300K1I5L8COCQ8W36 | Long             | EC               | CORP              | US        |    115333 | NS      | $1095663.50   | 0.18%             |  |  |  | No            |                  1 | On Loan: —       |
| Apellis Pharmaceuticals Inc         | Apellis Pharmaceuticals Inc COM USD0.0001                           | CUSIP: 03753U106<br>LEI: 254900HS0ZFRXXSB9D42 | Long             | EC               | CORP              | US        |    163008 | NS      | $3131383.68   | 0.52%             |  |  |  | No            |                  1 | On Loan: —       |
| Apogee Therapeutics Inc.            | APOGEE THERAPEUTICS INC                                             | CUSIP: 03770N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     38909 | NS      | $1527178.25   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Arbutus Biopharma Corp.             | ARBUTUS BIOPHARMA CORP                                              | CUSIP: 03879J100<br>LEI: 25490064CESONAUIAS23 | Long             | EC               | CORP              | CA        |     74980 | NS      | $266179.00    | 0.04%             |  |  |  | No            |                  1 | On Loan: —       |
| Arcellx Inc                         | Arcellx Inc COMMON STOCK                                            | CUSIP: 03940C100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     52937 | NS      | $3438258.15   | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Ardelyx Inc                         | ARDELYX INC                                                         | CUSIP: 039697107<br>LEI: 549300F542QR4SXHCY32 | Long             | EC               | CORP              | US        |    356011 | NS      | $1959840.56   | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcus Biosciences Inc.              | Arcus Biosciences Inc. COM USD0.0001                                | CUSIP: 03969F109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    101639 | NS      | $889341.25    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcutis Biotherapeutics Inc.        | ARCUTIS BIOTHERAPEUTICS INC                                         | CUSIP: 03969K108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    145119 | NS      | $2163724.29   | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcturus Therapeutics Holdings Inc  | Arcturus Therapeutics Holdings Inc COM                              | CUSIP: 03969T109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     44774 | NS      | $573554.94    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| ArriVent BioPharma Inc.             | ARRIVENT BIOPHARMA INC                                              | CUSIP: 04272N102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15761 | NS      | $335078.86    | 0.06%             |  |  |  | No            |                  1 | On Loan: —       |
| Arrowhead Pharmaceuticals Inc       | Arrowhead Pharmaceuticals Inc COM USD0.001                          | CUSIP: 04280A100<br>LEI: 549300O3CSB8T7OZ3D66 | Long             | EC               | CORP              | US        |    174443 | NS      | $2423013.27   | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.            | Avidity Biosciences Inc. COM                                        | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    131050 | NS      | $4278782.50   | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Beam Therapeutics Inc.              | Beam Therapeutics Incorporation COM                                 | CUSIP: 07373V105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    108477 | NS      | $2161946.61   | 0.36%             |  |  |  | No            |                  1 | On Loan: —       |
| BioCryst Pharmaceuticals Inc.       | BioCryst Pharmaceuticals Inc. COM USD0.01                           | CUSIP: 09058V103<br>LEI: 549300Z0INP1Z5NP6J59 | Long             | EC               | CORP              | US        |    229227 | NS      | $2028658.95   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Biomarin Pharmaceutical Inc.        | Biomarin Pharmaceutical Inc. COM USD0.001                           | CUSIP: 09061G101<br>LEI: NSLL8ITTRR0J5HEMR848 | Long             | EC               | CORP              | US        |    118497 | NS      | $7547073.93   | 1.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Biogen Inc                          | Biogen Inc COM USD0.0005                                            | CUSIP: 09062X103<br>LEI: W8J5WZB5IY3K0NDQT671 | Long             | EC               | CORP              | US        |     69754 | NS      | $8445814.32   | 1.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Blueprint Medicines Corporation     | Blueprint Medicines Corporation COM USD0.001                        | CUSIP: 09627Y109<br>LEI: 5299004CC60O9T5HVM83 | Long             | EC               | CORP              | US        |     89875 | NS      | $8043812.50   | 1.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Bridgebio Pharma Inc                | Bridgebio Pharma Inc COM USD0.001                                   | CUSIP: 10806X102<br>LEI: 54930028YG89VTY2J114 | Long             | EC               | CORP              | US        |    182897 | NS      | $7015928.92   | 1.16%             |  |  |  | No            |                  1 | On Loan: —       |
| Capricor Therapeutics Inc           | CAPRICOR THERAPEUTICS INC                                           | CUSIP: 14070B309<br>LEI: 5493002ZI5PDLXV4DX14 | Long             | EC               | CORP              | US        |     75105 | NS      | $953833.50    | 0.16%             |  |  |  | No            |                  1 | On Loan: —       |
| Caredx Inc                          | Caredx Inc COM USD0.001                                             | CUSIP: 14167L103<br>LEI: 549300NQOFKL9NON0U06 | Long             | EC               | CORP              | US        |     89883 | NS      | $1517225.04   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Catalyst Pharmaceuticals Inc.       | Catalyst Pharmaceuticals Inc. COM STK USD0.001                      | CUSIP: 14888U101<br>LEI: 5299004O0R5XXLA5H544 | Long             | EC               | CORP              | US        |     94741 | NS      | $2301258.89   | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Celldex Therapeutics Inc.           | Celldex Therapeutics Inc. COM USD0.001(POST REV SPLT                | CUSIP: 15117B202<br>LEI: 529900F018DMYWH38923 | Long             | EC               | CORP              | US        |     98019 | NS      | $2041735.77   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| CG Oncology Inc.                    | CG ONCOLOGY INC                                                     | CUSIP: 156944100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     64639 | NS      | $1741374.66   | 0.29%             |  |  |  | No            |                  1 | On Loan: —       |
| Cogent Biosciences Inc.             | Cogent Biosciences Inc. COM                                         | CUSIP: 19240Q201<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    151723 | NS      | $790476.83    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Crinetics Pharmaceuticals Inc.      | Crinetics Pharmaceuticals Inc. COM USD0.001                         | CUSIP: 22663K107<br>LEI: 549300Q1CNFXK5P31D28 | Long             | EC               | CORP              | US        |     92411 | NS      | $3085603.29   | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Cullinan Therapeutics Inc           | CULLINAN ONCOLOGY INC                                               | CUSIP: 230031106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     49727 | NS      | $411739.56    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Cytokinetics Inc                    | Cytokinetics Inc COM USD0.001                                       | CUSIP: 23282W605<br>LEI: 549300BE5DY1HW7IHA64 | Long             | EC               | CORP              | US        |    133450 | NS      | $5716998.00   | 0.95%             |  |  |  | No            |                  1 | On Loan: —       |
| Day One Biopharmaceuticals Inc.     | Day One Biopharmaceuticals Inc. COM                                 | CUSIP: 23954D109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    107832 | NS      | $838932.96    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Denali Therapeutics Inc             | Denali Therapeutics Inc COM USD0.01                                 | CUSIP: 24823R105<br>LEI: 549300ZTQ2HO18L3Q830 | Long             | EC               | CORP              | US        |    116846 | NS      | $1945485.90   | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Dianthus Therapeutics Inc           | DIANTHUS THERAPEUTICS INC                                           | CUSIP: 252828108<br>LEI: 549300Z3N6ODPCOFC890 | Long             | EC               | CORP              | US        |     21600 | NS      | $471960.00    | 0.08%             |  |  |  | No            |                  1 | On Loan: —       |
| Disc Medicine Inc                   | DISC MEDICINE INC                                                   | CUSIP: 254604101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     30798 | NS      | $1522037.16   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Dynavax Technologies Corp.          | Dynavax Technologies Corp. COM USD0.001                             | CUSIP: 268158201<br>LEI: 549300T5URIXWDILX594 | Long             | EC               | CORP              | US        |    148736 | NS      | $1747648.00   | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Dyne Therapeutics Inc.              | DYNE THERAPEUTICS INC                                               | CUSIP: 26818M108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    133339 | NS      | $1572066.81   | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| 89bio Inc.                          | 89BIO INC                                                           | CUSIP: 282559103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    124191 | NS      | $996011.82    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Emergent Biosolutions Inc           | EMERGENT BIOSOLUTIONS INC                                           | CUSIP: 29089Q105<br>LEI: 549300YW6TUOX8BE5103 | Long             | EC               | CORP              | US        |    100539 | NS      | $536878.26    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Exact Sciences Corporation          | Exact Sciences Corporation COM USD0.01                              | CUSIP: 30063P105<br>LEI: 549300VM3X1FYD4N9Z14 | Long             | EC               | CORP              | US        |    211014 | NS      | $9630678.96   | 1.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Exelixis, Inc.                      | Exelixis, Inc. COM USD0.001                                         | CUSIP: 30161Q104<br>LEI: 254900PJQB59O2O95R37 | Long             | EC               | CORP              | US        |    208283 | NS      | $8154279.45   | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Geron Corp.                         | Geron Corp. COM USD0.001                                            | CUSIP: 374163103<br>LEI: 549300T282D7Z2YESL90 | Long             | EC               | CORP              | US        |    797756 | NS      | $1124835.96   | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Gilead Sciences Inc.                | Gilead Sciences, Inc. COM USD0.001                                  | CUSIP: 375558103<br>LEI: 549300WTZWR07K8MNV44 | Long             | EC               | CORP              | US        |     85802 | NS      | $9141345.08   | 1.52%             |  |  |  | No            |                  1 | On Loan: No      |
| GRAIL Inc                           | GRAIL INC                                                           | CUSIP: 384747101<br>LEI: 2549009XSNESW3VE8024 | Long             | EC               | CORP              | US        |     55102 | NS      | $1900743.49   | 0.32%             |  |  |  | No            |                  1 | On Loan: —       |
| Halozyme Therapeutics Inc           | Halozyme Therapeutics Inc COM USD0.001                              | CUSIP: 40637H109<br>LEI: 529900242I3SV9AGM753 | Long             | EC               | CORP              | US        |    100544 | NS      | $6175412.48   | 1.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Humacyte Inc.                       | HUMACYTE INC                                                        | CUSIP: 44486Q103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    167406 | NS      | $242738.70    | 0.04%             |  |  |  | No            |                  1 | On Loan: —       |
| IDEAYA Biosciences Inc.             | IDEAYA Biosciences Inc. COM USD0.0001                               | CUSIP: 45166A102<br>LEI: 549300ULW08F62IJML11 | Long             | EC               | CORP              | US        |    109847 | NS      | $2211220.11   | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| ImmunityBio Inc                     | IMMUNITYBIO INC                                                     | CUSIP: 45256X103<br>LEI: 529900J9XLBR4HFL4B83 | Long             | EC               | CORP              | US        |    293683 | NS      | $737144.33    | 0.12%             |  |  |  | No            |                  1 | On Loan: —       |
| Immunome Inc                        | IMMUNOME INC                                                        | CUSIP: 45257U108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     92724 | NS      | $815043.96    | 0.14%             |  |  |  | No            |                  1 | On Loan: —       |
| Immunovant Inc                      | Immunovant Inc COM                                                  | CUSIP: 45258J102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    125638 | NS      | $2029053.70   | 0.34%             |  |  |  | No            |                  1 | On Loan: —       |
| Incyte Corporation.                 | Incyte Corporation. COM USD0.001                                    | CUSIP: 45337C102<br>LEI: 549300Z4WN6JVZ3T4680 | Long             | EC               | CORP              | US        |    144401 | NS      | $9048166.66   | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Insmed Inc                          | Insmed Inc COM USD0.01                                              | CUSIP: 457669307<br>LEI: 529900Q55QAG41CCAC11 | Long             | EC               | CORP              | US        |    132098 | NS      | $9511056.00   | 1.58%             |  |  |  | No            |                  1 | On Loan: No      |
| INHIBRX INC CVR                     | INHIBRX INC CVR                                                     | CUSIP: 457CVR017<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    164173 | NS      | $0.00         | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| Intellia Therapeutics Inc           | Intellia Therapeutics Inc COM USD0.0001                             | CUSIP: 45826J105<br>LEI: 5493002T5BCPE5DZC723 | Long             | EC               | CORP              | US        |    162953 | NS      | $1445393.11   | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Ionis Pharmaceuticals, Inc.         | Ionis Pharmaceuticals, Inc. COM USD0.001                            | CUSIP: 462222100<br>LEI: 549300SI4ZGLG0BLUZ92 | Long             | EC               | CORP              | US        |    159478 | NS      | $4897569.38   | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Iovance Biotherapeutics Inc         | Iovance Biotherapeutics Inc COM USD0.000041666                      | CUSIP: 462260100<br>LEI: 2549002L8WT2ACU5Q878 | Long             | EC               | CORP              | US        |    381437 | NS      | $1369358.83   | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Janux Therapeutics Inc              | JANUX THERAPEUTICS INC                                              | CUSIP: 47103J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     50933 | NS      | $1690975.60   | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| KalVista Pharmaceuticals Inc.       | KALVISTA PHARMACEUTICALS INC                                        | CUSIP: 483497103<br>LEI: 529900Q7IDA3WUGRZA51 | Long             | EC               | CORP              | US        |     23768 | NS      | $327285.36    | 0.05%             |  |  |  | No            |                  1 | On Loan: —       |
| Keros Therapeutics Inc.             | Keros Therapeutics Inc. COM                                         | CUSIP: 492327101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     50062 | NS      | $722895.28    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Krystal Biotech Inc                 | Krystal Biotech Inc COM USD0.00001                                  | CUSIP: 501147102<br>LEI: 549300YONUY68210OE41 | Long             | EC               | CORP              | US        |     21872 | NS      | $3715615.36   | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Kura Oncology Inc.                  | Kura Oncology Incorporation COM USD0.0001                           | CUSIP: 50127T109<br>LEI: 5493005QORQTZCZYOP39 | Long             | EC               | CORP              | US        |    118638 | NS      | $778265.28    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Kymera Therapeutics Inc             | Kymera Therapeutics Inc COM                                         | CUSIP: 501575104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     53385 | NS      | $1829503.95   | 0.30%             |  |  |  | No            |                  1 | On Loan: —       |
| Madrigal Pharmaceuticals Inc        | Madrigal Pharmaceuticals Inc USD0.0001                              | CUSIP: 558868105<br>LEI: 549300CHIMT2EUSNJB60 | Long             | EC               | CORP              | US        |     27064 | NS      | $9036940.24   | 1.50%             |  |  |  | No            |                  1 | On Loan: —       |
| Mannkind Corporation                | Mannkind Corporation COM USD0.01                                    | CUSIP: 56400P706<br>LEI: 213800GFBCNY1ZFST722 | Long             | EC               | CORP              | US        |    238837 | NS      | $1203738.48   | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| MiMedx Group Inc.                   | MiMedx Group Inc. COM USD0.001                                      | CUSIP: 602496101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     59325 | NS      | $408156.00    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Mirum Pharmaceuticals Inc.          | Mirum Pharmaceuticals Inc. COM USD0.0001                            | CUSIP: 604749101<br>LEI: 254900YZKR40F7F27K81 | Long             | EC               | CORP              | US        |     46931 | NS      | $2039151.95   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Moderna Inc                         | Moderna Inc COM USD0.0001                                           | CUSIP: 60770K107<br>LEI: 549300EI6OKH5K5Q2G38 | Long             | EC               | CORP              | US        |    279362 | NS      | $7972991.48   | 1.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Myriad Genetics Inc.                | Myriad Genetics Inc. COM USD0.01                                    | CUSIP: 62855J104<br>LEI: 52990044IHUOUMETT163 | Long             | EC               | CORP              | US        |    132844 | NS      | $984374.04    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Natera Inc.                         | Natera Incorporation COM USD0.0001                                  | CUSIP: 632307104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     67698 | NS      | $10217659.14  | 1.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Neurocrine Biosciences Inc          | Neurocrine Biosciences Inc COM USD0.001                             | CUSIP: 64125C109<br>LEI: 549300FECER0XBN49756 | Long             | EC               | CORP              | US        |     89666 | NS      | $9656131.54   | 1.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Novavax Inc                         | NOVAVAX INC                                                         | CUSIP: 670002401<br>LEI: 529900J4GJHPEPQ23205 | Long             | EC               | CORP              | US        |    251302 | NS      | $1676184.34   | 0.28%             |  |  |  | No            |                  1 | On Loan: —       |
| Nuvalent Inc                        | NUVALENT INC                                                        | CUSIP: 670703107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     46320 | NS      | $3555060.00   | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Nurix Therapeutics Inc.             | NURIX THERAPEUTICS INC                                              | CUSIP: 67080M103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     99617 | NS      | $1148584.01   | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| OmniAb Inc.                         | OmniAb Inc. USD0.0001 A (USD12.5 VESTING)                           | CUSIP: 68218J202<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14783 | NS      | $0.00         | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| OmniAb Inc.                         | OmniAb Inc. USD0.0001 A (USD15 VESTING)                             | CUSIP: 68218J301<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14783 | NS      | $0.00         | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Organogenesis Holdings Inc.         | ORGANOGENESIS HOLDINGS INC                                          | CUSIP: 68621F102<br>LEI: 549300RLC2O821TU6941 | Long             | EC               | CORP              | US        |     28951 | NS      | $142149.41    | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| ORIC Pharmaceuticals Inc.           | ORIC PHARMACEUTICALS INC                                            | CUSIP: 68622P109<br>LEI: 54930033FJRNEYBW0L25 | Long             | EC               | CORP              | US        |     70061 | NS      | $400048.31    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| PTC Therapeutics, Inc.              | PTC Therapeutics, Inc. COM USD0.001                                 | CUSIP: 69366J200<br>LEI: 549300UJLWOIWFDGB318 | Long             | EC               | CORP              | US        |     51632 | NS      | $2573338.88   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Praxis Precision Medicines Inc      | PRAXIS PRECISION MEDICINES I                                        | CUSIP: 74006W207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26465 | NS      | $996142.60    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Protagonist Therapeutics Inc        | Protagonist Therapeutics Inc COM USD0.00001                         | CUSIP: 74366E102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     40043 | NS      | $1834770.26   | 0.30%             |  |  |  | No            |                  1 | On Loan: —       |
| Recursion Pharmaceuticals Inc       | Recursion Pharmaceuticals Inc CL A                                  | CUSIP: 75629V104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    541499 | NS      | $3026979.41   | 0.50%             |  |  |  | No            |                  1 | On Loan: —       |
| Regeneron Pharmaceuticals, Inc.     | Regeneron Pharmaceuticals, Inc. COM USD0.001                        | CUSIP: 75886F107<br>LEI: 549300RCBFWIRX3HYQ56 | Long             | EC               | CORP              | US        |     14180 | NS      | $8490416.80   | 1.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Relay Therapeutics Inc              | Relay Therapeutics Inc COM                                          | CUSIP: 75943R102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    185555 | NS      | $617898.15    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Replimune Group Inc                 | REPLIMUNE GROUP INC                                                 | CUSIP: 76029N106<br>LEI: 549300AY704NEIRC4K64 | Long             | EC               | CORP              | US        |     71127 | NS      | $695622.06    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc            | Revolution Medicines Inc COM                                        | CUSIP: 76155X100<br>LEI: 549300JU3X0LBCQ65892 | Long             | EC               | CORP              | US        |    147880 | NS      | $5971394.40   | 0.99%             |  |  |  | No            |                  1 | On Loan: No      |
| Rhythm Pharmaceuticals Inc          | Rhythm Pharmaceuticals Inc COM USD0.001                             | CUSIP: 76243J105<br>LEI: 529900JPNWXQ8DPLA551 | Long             | EC               | CORP              | US        |     52239 | NS      | $3405460.41   | 0.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Rigel Pharmaceuticals Inc.          | RIGEL PHARMACEUTICALS INC                                           | CUSIP: 766559702<br>LEI: 529900KPTNUS5J8K9K66 | Long             | EC               | CORP              | US        |     19354 | NS      | $378564.24    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Rocket Pharmaceuticals Inc          | Rocket Pharmaceuticals Inc COM USD0.01                              | CUSIP: 77313F106<br>LEI: 549300R7VV82YUIN4Y93 | Long             | EC               | CORP              | US        |    141040 | NS      | $1076135.20   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Sana Biotechnology Inc              | SANA BIOTECHNOLOGY INC                                              | CUSIP: 799566104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    176560 | NS      | $335464.00    | 0.06%             |  |  |  | No            |                  1 | On Loan: —       |
| Sarepta Therapeutics Inc            | Sarepta Therapeutics Inc COM USD0.0001                              | CUSIP: 803607100<br>LEI: 549300IKDPIED8J8IG21 | Long             | EC               | CORP              | US        |     88188 | NS      | $5502931.20   | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Scholar Rock Holding Corp.          | SCHOLAR ROCK HOLDING CORP                                           | CUSIP: 80706P103<br>LEI: 549300Z1FLPGL4OWJ459 | Long             | EC               | CORP              | US        |    101052 | NS      | $3325621.32   | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Scilex Holding Co                   | SCILEX HOLDING CO                                                   | CUSIP: 80880W205<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17718 | NS      | $83983.32     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| ARS Pharmaceuticals Inc             | ARS PHARMACEUTICALS INC                                             | CUSIP: 82835W108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     68164 | NS      | $952251.08    | 0.16%             |  |  |  | No            |                  1 | On Loan: —       |
| Soleno Therapeutics Inc             | SOLENO THERAPEUTICS INC                                             | CUSIP: 834203309<br>LEI: 5493003LCY77N7FXK844 | Long             | EC               | CORP              | US        |     41943 | NS      | $3139852.98   | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Springworks Therapeutics Inc        | Springworks Therapeutics Inc COM USD0.0001                          | CUSIP: 85205L107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    100383 | NS      | $4647732.90   | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Stoke Therapeutics Inc              | STOKE THERAPEUTICS INC                                              | CUSIP: 86150R107<br>LEI: 549300RD25SS2DIJHJ67 | Long             | EC               | CORP              | US        |     55834 | NS      | $544939.84    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Summit Therapeutics Inc.            | SUMMIT THERAPEUTICS INC                                             | CUSIP: 86627T108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    207887 | NS      | $5014234.44   | 0.83%             |  |  |  | No            |                  1 | On Loan: —       |
| Syndax Pharmaceuticals Inc.         | Syndax Pharmaceuticals Inc. COM USD0.0001                           | CUSIP: 87164F105<br>LEI: 529900OF94TRHKZVBS87 | Long             | EC               | CORP              | US        |    155215 | NS      | $2196292.25   | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| TG Therapeutics Inc                 | TG Therapeutics Inc COM USD0.001                                    | CUSIP: 88322Q108<br>LEI: 529900X21UKABN9NJ529 | Long             | EC               | CORP              | US        |    164564 | NS      | $7489307.64   | 1.24%             |  |  |  | No            |                  1 | On Loan: —       |
| Travere Therapeutics Inc            | Travere Therapeutics Inc COM                                        | CUSIP: 89422G107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    131229 | NS      | $2730875.49   | 0.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Twist Bioscience Corporation        | Twist Bioscience Corporation COM USD0.00001                         | CUSIP: 90184D100<br>LEI: 549300T23BL59LCOH584 | Long             | EC               | CORP              | US        |     89835 | NS      | $3442477.20   | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Ultragenyx Pharmaceuticals Inc      | Ultragenyx Pharmaceuticals Inc COM USD0.001                         | CUSIP: 90400D108<br>LEI: 529900EV44GVDN1DCX77 | Long             | EC               | CORP              | US        |     80362 | NS      | $3132510.76   | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation     | United Therapeutics Corporation COM USD0.01                         | CUSIP: 91307C102<br>LEI: 5299005C4HZL4UWROC14 | Long             | EC               | CORP              | US        |     31842 | NS      | $9650991.78   | 1.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                         | Vaxcyte Inc COM                                                     | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |    119264 | NS      | $4274421.76   | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Veracyte Inc                        | Veracyte Inc COM USD0.001                                           | CUSIP: 92337F107<br>LEI: 529900ESWZRHXOW27Z37 | Long             | EC               | CORP              | US        |    100407 | NS      | $3062413.50   | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Vera Therapeutics Inc.              | VERA THERAPEUTICS INC                                               | CUSIP: 92337R101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     76809 | NS      | $1795026.33   | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Vericel Corporation                 | Vericel Corporation COM NPV                                         | CUSIP: 92346J108<br>LEI: 54930076YM3PLTDR4B91 | Long             | EC               | CORP              | US        |     43162 | NS      | $1641019.24   | 0.27%             |  |  |  | No            |                  1 | On Loan: —       |
| Vertex Pharmaceuticals Incorporated | Vertex Pharmaceuticals Incorporated COM USD0.01                     | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |     19738 | NS      | $10056511.00  | 1.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Verve Therapeutics Inc              | Verve Therapeutics Inc COM                                          | CUSIP: 92539P101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    101889 | NS      | $577710.63    | 0.10%             |  |  |  | No            |                  1 | On Loan: —       |
| Viking Therapeutics Inc.            | VIKING THERAPEUTICS INC                                             | CUSIP: 92686J106<br>LEI: 529900IVC72YPFA25M37 | Long             | EC               | CORP              | US        |    166293 | NS      | $4800878.91   | 0.80%             |  |  |  | No            |                  1 | On Loan: —       |
| VIR Biotechnology Inc               | VIR Biotechnology Inc COM                                           | CUSIP: 92764N102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    154852 | NS      | $947694.24    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Viridian Therapeutics Inc           | Viridian Therapeutics Inc COM                                       | CUSIP: 92790C104<br>LEI: 549300C775BNYF550E70 | Long             | EC               | CORP              | US        |     95820 | NS      | $1298361.00   | 0.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Xencor Inc                          | Xencor Inc COM USD0.01                                              | CUSIP: 98401F105<br>LEI: 549300V5IF65437JKG30 | Long             | EC               | CORP              | US        |     78495 | NS      | $865014.90    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Alkermes PLC                        | Alkermes PLC COM USD0.01                                            | CUSIP: G01767105<br>LEI: N/A                  | Long             | EC               | CORP              | IE        |    136131 | NS      | $3916488.87   | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Biohaven Ltd                        | Biohaven Ltd COM NPV                                                | CUSIP: G1110E107<br>LEI: N/A                  | Long             | EC               | CORP              | VG        |    108308 | NS      | $2395772.96   | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Prothena Corporation PLC            | Prothena Corporation PLC USD0.01                                    | CUSIP: G72800108<br>LEI: 5299009ZXQ8NIIJ8OL30 | Long             | EC               | CORP              | IE        |     39785 | NS      | $366022.00    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Roivant Sciences Ltd                | ROIVANT SCIENCES LTD                                                | CUSIP: G76279101<br>LEI: 549300DL466NY5J6U724 | Long             | EC               | CORP              | BM        |    448569 | NS      | $5212371.78   | 0.86%             |  |  |  | No            |                  1 | On Loan: —       |
| CRISPR Therapeutics AG              | CRISPR Therapeutics AG COM CHF0.03                                  | CUSIP: H17182108<br>LEI: 506700O6IRRIQLT3W370 | Long             | EC               | CORP              | CH        |    132141 | NS      | $5109892.47   | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Uniqure N.V.                        | UNIQURE NV                                                          | CUSIP: N90064101<br>LEI: 724500RPCTZLFYJZ3S98 | Long             | EC               | CORP              | NL        |     74743 | NS      | $1103206.68   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| DREYFUS GOVERNMENT CASH MANAGE      | DREYFUS GOV'T CASH MGMT -INSTITUTIONAL CUSIP 262006208 DGCXX (#289) | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | RF                | US        | 162914303 | PA      | $162914303.07 | 27.02%            |  |  |  | No            |                  1 | On Loan: No      |
| GOLDMAN FINANCIAL                   | GOLDMAN FINL SQ TRSRY INST 506                                      | CUSIP: N/A<br>LEI: 549300DAH6N80PM31E83       | Long             | STIV             | RF                | US        |  95586139 | PA      | $95586138.56  | 15.85%            |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $26842564.58  | 4.45%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $-16798881.05 | -2.79%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $-16696463.18 | -2.77%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $-28491477.50 | -4.73%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $-3515594.31  | -0.58%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $-27092639.35 | -4.49%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                 | S&P BIOTECHNOLOGY SELECT INDUSTRY INDEX SWAP                        | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |         1 | NC      | $3214222.63   | 0.53%             |  |  |  | No            |                  2 | On Loan: No      |
| DREYFUS                             | DREYFUS TRSRY SECURITIES CASH MGMT                                  | CUSIP: S99991620<br>LEI: N/A                  | Long             | STIV             | RF                | US        |  31366143 | PA      | $31366142.71  | 5.20%             |  |  |  | No            |                  1 | On Loan: No      |
| GOLDMAN SACHS LIQ ES FD A           | GOLDMAN SACHS FIN GOV 465 INSTITUT                                  | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | RF                | US        |  18721150 | PA      | $18721150.00  | 3.10%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2025-06-27

**Name of Applicant:** Direxion Shares ETF Trust

**Signature:** Patrick Rudnick

**Name of Signer:** Patrick Rudnick

**Title:** Principal Executive Officer