# EDGAR Filing Document

**Accession Number:** 0000022370
**File Stem:** 0000022370-26-000088
**Filing Date:** 2026-5
**Character Count:** 29274
**Document Hash:** 2575453c036263b58666b3a33f94278c
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0000022370-26-000088.hdr.sgml**: 20260529

**ACCESSION NUMBER**: 0000022370-26-000088

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20260331

**FILED AS OF DATE**: 20260529

**DATE AS OF CHANGE**: 20260529

**PERIOD START**: 20260331

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JOHN HANCOCK INVESTMENT TRUST
- **CENTRAL INDEX KEY:** 0000022370

**ORGANIZATION NAME:**
- **EIN:** 746035056
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 1031

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-00560
- **FILM NUMBER:** 261040571

**BUSINESS ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116
- **BUSINESS PHONE:** 617-663-3000

**MAIL ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** HANCOCK JOHN INVESTMENT TRUST /MA/
- **DATE OF NAME CHANGE:** 19950131

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** TRANSAMERICA INVESTMENT TRUST
- **DATE OF NAME CHANGE:** 19950131

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** CRITERION INCOME TRUST
- **DATE OF NAME CHANGE:** 19890820

## Series and Classes Contracts Data

### John Hancock Mid Cap Growth Fund (Series ID: S000072133)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000227913 | Class NAV    | JACFX           |
| C000227914 | Class I      | JACBX           |
| C000227915 | Class R6     | JACEX           |
| C000227916 | Class A      | JACJX           |
| C000227917 | Class C      | JACLX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** John Hancock Investment Trust

- **b. Investment Company Act file number:** 811-00560

- **c. CIK number of Registrant:** 0000022370

- **d. LEI of Registrant:** 549300WRGYBW55IKGU23

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 200 Berkeley Street

  - **City:** Boston

  - **State:** MA

  - **Foreign country:** US

  - **Zip / Postal Code:** 02116

  - **Telephone number:** 617-543-9634

**Item A.2. Information about the Series.**

- **a. Name of Series:** Mid Cap Growth Fund

- **b. EDGAR series identifier (if any):** S000072133

- **c. LEI of Series:** 5493008CVP7HL6CDJ403

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2026-03-31

- **b. Date as of which information is reported:** 2026-03-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $1146792945.62

**Total Liabilities:** $204214.20

**Net Assets:** $1146588731.42

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000227913 | -0.39%               | 2.70%                | -6.14%               |
| Class ID C000227914 | -0.45%               | 2.72%                | -6.13%               |
| Class ID C000227915 | -0.39%               | 2.76%                | -6.20%               |
| Class ID C000227916 | -0.40%               | 2.69%                | -6.20%               |
| Class ID C000227917 | -0.47%               | 2.61%                | -6.20%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $28476196.30             | $-32357358.40                              |
| Month 2  | $-10257048.55            | $43505153.70                               |
| Month 3  | $-4236576.65             | $-70723575.38                              |

### Schedule of Portfolio Investments

| Name                                                        | Title                                                       | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Evercore Inc.                                               | EVERCORE INC COMMON STOCK USD.01                            | CUSIP: 29977A105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     60252 | NS      | $17985824.52  | 1.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Expedia Group Inc.                                          | EXPEDIA GROUP INC COMMON STOCK USD.001                      | CUSIP: 30212P303<br>LEI: CI7MUJI4USF3V0NJ1H64 | Long             | EC               | CORP              | US        |     47544 | NS      | $10977434.16  | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Fair Isaac Corporation                                      | FAIR ISAAC CORP COMMON STOCK USD.01                         | CUSIP: 303250104<br>LEI: RI6HET5SJUEY30V1PS26 | Long             | EC               | CORP              | US        |      7776 | NS      | $8301191.04   | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Five Below Inc.                                             | FIVE BELOW INC COMMON STOCK USD.01                          | CUSIP: 33829M101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     61508 | NS      | $14053347.84  | 1.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Medpace Holdings Inc.                                       | MEDPACE HOLDINGS INC COMMON STOCK USD.01                    | CUSIP: 58506Q109<br>LEI: 549300H8TYEUVTW14A54 | Long             | EC               | CORP              | US        |     25066 | NS      | $12036442.54  | 1.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Monster Beverage Corporation                                | MONSTER BEVERAGE CORP COMMON STOCK USD.005                  | CUSIP: 61174X109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     75675 | NS      | $5483410.50   | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| FTAI Aviation Ltd.                                          | FTAI AVIATION LTD COMMON STOCK USD1.0                       | CUSIP: 34960P101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    111196 | NS      | $27243020.00  | 2.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Guidewire Software Inc.                                     | GUIDEWIRE SOFTWARE INC COMMON STOCK USD.0001                | CUSIP: 40171V100<br>LEI: 549300WXSG0J8IXZCK32 | Long             | EC               | CORP              | US        |    109741 | NS      | $16412863.96  | 1.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Hilton Worldwide Holdings Inc.                              | HILTON WORLDWIDE HOLDINGS INC COMMON STOCK USD.01           | CUSIP: 43300A203<br>LEI: 549300HVGPK36ICB0B89 | Long             | EC               | CORP              | US        |     22778 | NS      | $6926334.24   | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Howmet Aerospace Inc.                                       | HOWMET AEROSPACE INC COMMON STOCK                           | CUSIP: 443201108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    187502 | NS      | $43211710.92  | 3.77%             |  |  |  | No            |                  1 | On Loan: No      |
| JH COLLATERAL                                               | JH COLLATERAL                                               | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | RF                | US        |    255484 | NS      | $2555276.15   | 0.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Tapestry Inc.                                               | TAPESTRY INC COMMON STOCK USD.01                            | CUSIP: 876030107<br>LEI: 549300LJNVY5SW3VTN33 | Long             | EC               | CORP              | US        |     67665 | NS      | $9548208.15   | 0.83%             |  |  |  | No            |                  1 | On Loan: No      |
| Targa Resources Corp.                                       | TARGA RESOURCES CORP COMMON STOCK USD.001                   | CUSIP: 87612G101<br>LEI: 5493003QENHHS261UR94 | Long             | EC               | CORP              | US        |     86047 | NS      | $21574564.31  | 1.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc.                                               | ILLUMINA INC COMMON STOCK USD.01                            | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |    125873 | NS      | $15515105.98  | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Interactive Brokers Group Inc.                              | INTERACTIVE BROKERS GROUP INC COMMON STOCK USD.01           | CUSIP: 45841N107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    128849 | NS      | $8641902.43   | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| NRG Energy Inc.                                             | NRG ENERGY INC COMMON STOCK USD.01                          | CUSIP: 629377508<br>LEI: 5E2UPK5SW04M13XY7I38 | Long             | EC               | CORP              | US        |    172653 | NS      | $25231509.42  | 2.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Natera Inc.                                                 | NATERA INC COMMON STOCK                                     | CUSIP: 632307104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    197719 | NS      | $39541822.81  | 3.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Cameco Corporation                                          | CAMECO CORP COMMON STOCK                                    | CUSIP: 13321L108<br>LEI: N4BHALMXL3GMZKCUON55 | Long             | EC               | CORP              | CA        |    119338 | NS      | $12961300.18  | 1.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Carpenter Technology Corporation                            | CARPENTER TECHNOLOGY CORP COMMON STOCK USD5.0               | CUSIP: 144285103<br>LEI: DX6I6ZD3X5WNNCDJKP85 | Long             | EC               | CORP              | US        |     31979 | NS      | $12604522.85  | 1.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Carvana Co.                                                 | CARVANA CO COMMON STOCK USD.001                             | CUSIP: 146869102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     52995 | NS      | $16660568.10  | 1.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Cava Group Inc.                                             | CAVA GROUP INC COMMON STOCK USD.0001                        | CUSIP: 148929102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     78822 | NS      | $6376699.80   | 0.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Tradeweb Markets Inc.                                       | TRADEWEB MARKETS INC COMMON STOCK USD.00001                 | CUSIP: 892672106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    116810 | NS      | $13743864.60  | 1.20%             |  |  |  | No            |                  1 | On Loan: No      |
| EchoStar Corporation                                        | ECHOSTAR CORP COMMON STOCK USD.001                          | CUSIP: 278768106<br>LEI: 549300EE20907QZ9GT38 | Long             | EC               | CORP              | US        |    227977 | NS      | $26689267.39  | 2.33%             |  |  |  | No            |                  1 | On Loan: No      |
| The Ensign Group Inc.                                       | ENSIGN GROUP INC COMMON STOCK USD.001                       | CUSIP: 29358P101<br>LEI: 5493003K4HEEA8Q0BC72 | Long             | EC               | CORP              | US        |    107242 | NS      | $21609263.00  | 1.88%             |  |  |  | No            |                  1 | On Loan: No      |
| EquipmentShare.com Inc                                      | EQUIPMENTSHARE.COM INC COMMON STOCK USD.0000013             | CUSIP: 29445S100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    128118 | NS      | $2609763.66   | 0.23%             |  |  |  | No            |                  1 | On Loan: —       |
| Marriott International Inc.                                 | MARRIOTT INTERNATIONAL INC/MD COMMON STOCK USD.01           | CUSIP: 571903202<br>LEI: 225YDZ14ZO8E1TXUSU86 | Long             | EC               | CORP              | US        |     58589 | NS      | $19162704.23  | 1.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Royal Caribbean Cruises Ltd.                                | ROYAL CARIBBEAN CRUISES LTD COMMON STOCK USD.01             | CUSIP: V7780T103<br>LEI: K2NEH8QNVW44JIWK7Z55 | Long             | EC               | CORP              | US        |    154596 | NS      | $42541727.28  | 3.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Flex Ltd.                                                   | FLEX LTD COMMON STOCK                                       | CUSIP: Y2573F102<br>LEI: 549300EAQH74YHD07T53 | Long             | EC               | CORP              | US        |    428092 | NS      | $28022902.32  | 2.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Ollie's Bargain Outlet Holdings Inc.                        | OLLIE'S BARGAIN OUTLET HOLDING COMMON STOCK USD.001         | CUSIP: 681116109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    187794 | NS      | $17284559.76  | 1.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Quanta Services Inc.                                        | QUANTA SERVICES INC COMMON STOCK USD.00001                  | CUSIP: 74762E102<br>LEI: SHVRXXEACT60MMH07S24 | Long             | EC               | CORP              | US        |     46811 | NS      | $25700175.22  | 2.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Sandisk Corporation                                         | SANDISK CORP/DE COMMON STOCK USD.01                         | CUSIP: 80004C200<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21525 | NS      | $13675693.50  | 1.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Structure Therapeutics Inc.                                 | STRUCTURE THERAPEUTICS INC ADR USD.0001                     | CUSIP: 86366E106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    131476 | NS      | $6337143.20   | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Reddit Inc.                                                 | REDDIT INC COMMON STOCK USD.0001                            | CUSIP: 75734B100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    153388 | NS      | $20653694.20  | 1.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Roblox Corporation                                          | ROBLOX CORP COMMON STOCK USD.0001                           | CUSIP: 771049103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    246240 | NS      | $13927334.40  | 1.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Ross Stores Inc.                                            | ROSS STORES INC COMMON STOCK USD.01                         | CUSIP: 778296103<br>LEI: 549300ENZFLPGRDFZQ60 | Long             | EC               | CORP              | US        |     43100 | NS      | $9336753.00   | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| State Street Bank and Trust Company                         | SCC GOVERMENT MM GVXX 20301231 00001.0000000000             | CUSIP: 7839989D1<br>LEI: 571474TGEMMWANRLN572 | Long             | STIV             | RF                | US        |  13605226 | NS      | $13605225.94  | 1.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Samsara Inc.                                                | SAMSARA INC COMMON STOCK USD.0001                           | CUSIP: 79589L106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    627556 | NS      | $19887249.64  | 1.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Trimble Inc.                                                | TRIMBLE INC COMMON STOCK USD.001                            | CUSIP: 896239100<br>LEI: 549300E2MI7NSZFQWS19 | Long             | EC               | CORP              | US        |    333359 | NS      | $21745007.57  | 1.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Celestica Inc.                                              | CELESTICA INC COMMON STOCK                                  | CUSIP: 15101Q207<br>LEI: 549300J3CG5T6RZWLN53 | Long             | EC               | CORP              | CA        |     87126 | NS      | $24541651.68  | 2.14%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation                             | UNITED THERAPEUTICS CORP COMMON STOCK USD.01                | CUSIP: 91307C102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20537 | NS      | $12178030.26  | 1.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Veeva Systems Inc.                                          | VEEVA SYSTEMS INC COMMON STOCK USD.00001                    | CUSIP: 922475108<br>LEI: 549300YZEXPDB92M2F02 | Long             | EC               | CORP              | US        |    107902 | NS      | $18954065.32  | 1.65%             |  |  |  | No            |                  1 | On Loan: No      |
| VANGUARD MID-CAP GROWTH ETF VANGUARD MID-CAP GROWTH ETF     | VANGUARD MID-CAP GROWTH ETF VANGUARD MID-CAP GROWTH ETF     | CUSIP: 922908538<br>LEI: N/A                  | Long             | EC               | RF                | US        |     11869 | NS      | $3054487.15   | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertiv Holdings Co                                          | VERTIV HOLDINGS CO COMMON STOCK USD.0001                    | CUSIP: 92537N108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    237978 | NS      | $59632527.24  | 5.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Clean Harbors Inc.                                          | CLEAN HARBORS INC COMMON STOCK USD.01                       | CUSIP: 184496107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     46870 | NS      | $13439035.10  | 1.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Cloudflare Inc.                                             | CLOUDFLARE INC COMMON STOCK USD.001                         | CUSIP: 18915M107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    242882 | NS      | $50116271.88  | 4.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Cognex Corporation                                          | COGNEX CORP COMMON STOCK USD.002                            | CUSIP: 192422103<br>LEI: 549300JQTYZCLC43S226 | Long             | EC               | CORP              | US        |    203851 | NS      | $9986660.49   | 0.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Corning Incorporated                                        | CORNING INC COMMON STOCK USD.5                              | CUSIP: 219350105<br>LEI: 549300X2937PB0CJ7I56 | Long             | EC               | CORP              | US        |    290055 | NS      | $39438778.35  | 3.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Zillow Group Inc.                                           | ZILLOW GROUP INC COMMON STOCK USD.0001                      | CUSIP: 98954M200<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    209178 | NS      | $8655785.64   | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| ESSENCE HLDG GR PP PREFERRED STOCK                          | ESSENCE HLDG GR PP PREFERRED STOCK                          | CUSIP: ACI08CVD8<br>LEI: N/A                  | Long             | EP               | CORP              | US        |   2958957 | NS      | $1686605.49   | 0.15%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Lookout Inc.                                                | LOOKOUT INC. LOOKOUT INC. SERIES F                          | CUSIP: ACI08PY20<br>LEI: N/A                  | Long             | EP               | CORP              | US        |    392767 | NS      | $1280420.42   | 0.11%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Amer Sports Inc.                                            | AMER SPORTS INC COMMON STOCK                                | CUSIP: G0260P102<br>LEI: N/A                  | Long             | EC               | CORP              | FI        |    317973 | NS      | $10467671.16  | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Datadog Inc.                                                | DATADOG INC COMMON STOCK USD.00001                          | CUSIP: 23804L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    187522 | NS      | $22136972.10  | 1.93%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc.                                               | DOORDASH INC COMMON STOCK USD.00001                         | CUSIP: 25809K105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    113857 | NS      | $17095628.55  | 1.49%             |  |  |  | No            |                  1 | On Loan: No      |
| On Holding AG                                               | ON HOLDING AG COMMON STOCK CHF.1                            | CUSIP: H5919C104<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |    565050 | NS      | $19223001.00  | 1.68%             |  |  |  | No            |                  1 | On Loan: No      |
| DraftKings Inc.                                             | DRAFTKINGS INC COMMON STOCK USD.0001                        | CUSIP: 26142V105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1179330 | NS      | $25497114.60  | 2.22%             |  |  |  | No            |                  1 | On Loan: No      |
| AST SpaceMobile Inc.                                        | AST SPACEMOBILE INC COMMON STOCK USD.0001                   | CUSIP: 00217D100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    129610 | NS      | $10740780.70  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| argenx SE                                                   | ARGENX SE ADR                                               | CUSIP: 04016X101<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |     16875 | NS      | $12322968.75  | 1.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Aritzia Inc.                                                | ARITZIA INC COMMON STOCK                                    | CUSIP: 04045U102<br>LEI: N/A                  | Long             | EC               | CORP              | CA        |    216885 | NS      | $17698788.87  | 1.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Axon Enterprise Inc.                                        | AXON ENTERPRISE INC COMMON STOCK USD.00001                  | CUSIP: 05464C101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     69877 | NS      | $29676063.13  | 2.59%             |  |  |  | No            |                  1 | On Loan: No      |
| C.H. Robinson Worldwide Inc.                                | CH ROBINSON WORLDWIDE INC COMMON STOCK USD.1                | CUSIP: 12541W209<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     73686 | NS      | $12237034.02  | 1.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Ionis Pharmaceuticals Inc.                                  | IONIS PHARMACEUTICALS INC COMMON STOCK USD.001              | CUSIP: 462222100<br>LEI: 549300SI4ZGLG0BLUZ92 | Long             | EC               | CORP              | US        |     94101 | NS      | $7066044.09   | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| ISHARES RUSSELL MID-CAP GROWTH ISHARES RUSSELL MID-CAP GROW | ISHARES RUSSELL MID-CAP GROWTH ISHARES RUSSELL MID-CAP GROW | CUSIP: 464287481<br>LEI: 5493004I787UFE6AN650 | Long             | EC               | RF                | US        |     23953 | NS      | $3068858.36   | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| KKR and Co. Inc.                                            | KKR and CO INC COMMON STOCK USD.01                          | CUSIP: 48251W104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    127930 | NS      | $11833525.00  | 1.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Kratos Defense and Security Solutions Inc.                  | KRATOS DEFENSE and SECURITY SOLU COMMON STOCK USD.001       | CUSIP: 50077B207<br>LEI: 5493000EKBN0UY1F3F88 | Long             | EC               | CORP              | US        |    182914 | NS      | $12897266.14  | 1.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corporation                                   | LIBERTY MEDIA CORP-LIBERTY FOR TRACKING STK                 | CUSIP: 531229755<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    167315 | NS      | $14225121.30  | 1.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Life Time Group Holdings Inc.                               | LIFE TIME GROUP HOLDINGS INC COMMON STOCK USD.01            | CUSIP: 53190C102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    586440 | NS      | $15798693.60  | 1.38%             |  |  |  | No            |                  1 | On Loan: No      |
| MKS Inc.                                                    | MKS INC COMMON STOCK                                        | CUSIP: 55306N104<br>LEI: 549300KEQATBHAGVF505 | Long             | EC               | CORP              | US        |     59020 | NS      | $13563386.20  | 1.18%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2026-04-29

**Name of Applicant:** John Hancock Investment Trust

**Signature:** /s/Salvatore Schiavone

**Name of Signer:** Salvatore Schiavone

**Title:** Treasurer