# EDGAR Filing Document

**Accession Number:** 0000756913
**File Stem:** 0001193125-26-080766
**Filing Date:** 2026-2
**Character Count:** 64655
**Document Hash:** e1262a8200ac4dabfcc19ed784754ffe
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-26-080766.hdr.sgml**: 20260227

**ACCESSION NUMBER**: 0001193125-26-080766

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20251231

**FILED AS OF DATE**: 20260227

**DATE AS OF CHANGE**: 20260227

**PERIOD START**: 20251231

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JOHN HANCOCK VARIABLE INSURANCE TRUST
- **CENTRAL INDEX KEY:** 0000756913

**ORGANIZATION NAME:**
- **EIN:** 000000000
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 0630

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-04146
- **FILM NUMBER:** 26694997

**BUSINESS ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116
- **BUSINESS PHONE:** 617-663-3000

**MAIL ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JOHN HANCOCK TRUST
- **DATE OF NAME CHANGE:** 20050124

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** MANUFACTURERS INVESTMENT TRUST
- **DATE OF NAME CHANGE:** 19971022

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** NASL SERIES FUND INC
- **DATE OF NAME CHANGE:** 19881030

## Series and Classes Contracts Data

### Health Sciences Trust (Series ID: S000008239)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000022442 | NAV          | JAFMX           |
| C000022443 | Series I     | JEHSX           |
| C000022444 | Series II    | JAFKX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** John Hancock Variable Insurance Trust

- **b. Investment Company Act file number:** 811-04146

- **c. CIK number of Registrant:** 0000756913

- **d. LEI of Registrant:** Z5DUWKEPW78QKD87GA06

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 200 Berkeley Street

  - **City:** Boston

  - **State:** MA

  - **Foreign country:** US

  - **Zip / Postal Code:** 02116

  - **Telephone number:** 617-543-9634

**Item A.2. Information about the Series.**

- **a. Name of Series:** Health Sciences Trust

- **b. EDGAR series identifier (if any):** S000008239

- **c. LEI of Series:** K5HWWGQWUOYDF5BZA919

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-12-31

- **b. Date as of which information is reported:** 2025-12-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $246258501.04

**Total Liabilities:** $56249.84

**Net Assets:** $246202251.20

**Cash Not Reported:** $395.13

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000022442 | 8.42%                | 9.49%                | -2.04%               |
| Class ID C000022443 | 8.47%                | 9.46%                | -2.03%               |
| Class ID C000022444 | 8.40%                | 9.46%                | -2.06%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $7339746.79              | $11015346.70                               |
| Month 2  | $4660930.04              | $17502398.62                               |
| Month 3  | $5322098.44              | $-10419176.95                              |

### Schedule of Portfolio Investments

| Name                                              | Title                                                  | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Neurocrine Biosciences Inc.                       | NEUROCRINE BIOSCIENCES INC COMMON STOCK USD.001        | CUSIP: 64125C109<br>LEI: 549300FECER0XBN49756 | Long             | EC               | CORP              | US        |      5187 | NS      | $735672.21    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Nurix Therapeutics Inc.                           | NURIX THERAPEUTICS INC COMMON STOCK                    | CUSIP: 67080M103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     30800 | NS      | $584276.00    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvation Bio Inc.                                 | NUVATION BIO INC COMMON STOCK USD.0001                 | CUSIP: 67080N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     40900 | NS      | $366464.00    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Olema Pharmaceuticals Inc.                        | OLEMA PHARMACEUTICALS INC COMMON STOCK USD.0001        | CUSIP: 68062P106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17800 | NS      | $445000.00    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Orchestra Biomed Holdings Inc.                    | ORCHESTRA BIOMED HOLDINGS INC COMMON STOCK USD.0001    | CUSIP: 68572M106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13354 | NS      | $55419.10     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| ORIC Pharmaceuticals Inc.                         | ORIC PHARMACEUTICALS INC COMMON STOCK USD.0001         | CUSIP: 68622P109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26621 | NS      | $217759.78    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Oruka Therapeutics Inc.                           | ORUKA THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 687604108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8520 | NS      | $258241.20    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Palisade Bio Inc.                                 | PALISADE BIO INC COMMON STOCK USD.01                   | CUSIP: 696389402<br>LEI: 549300W6LIDX3I5XXT34 | Long             | EC               | CORP              | US        |     27162 | NS      | $63830.70     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Penumbra Inc.                                     | PENUMBRA INC COMMON STOCK USD.001                      | CUSIP: 70975L107<br>LEI: 549300RGE06P3ECANU72 | Long             | EC               | CORP              | US        |     11098 | NS      | $3450479.18   | 1.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Revvity Inc.                                      | REVVITY INC COMMON STOCK USD1.0                        | CUSIP: 714046109<br>LEI: 549300IKL1SDPFI7N655 | Long             | EC               | CORP              | US        |      6945 | NS      | $671928.75    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Sonova Holding AG                                 | SONOVA HOLDING AG COMMON STOCK CHF.05                  | CUSIP: 715603908<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |      2233 | NS      | $577602.56    | 0.23%             |  |  |  | No            |                  2 | On Loan: No      |
| Praxis Precision Medicines Inc.                   | PRAXIS PRECISION MEDICINES INC COMMON STOCK USD.0001   | CUSIP: 74006W207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5194 | NS      | $1530879.56   | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Prelude Therapeutics Incorporated                 | PRELUDE THERAPEUTICS INC COMMON STOCK USD.0001         | CUSIP: 74065P101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17428 | NS      | $50541.20     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Procept Biorobotics Corporation                   | PROCEPT BIOROBOTICS CORP COMMON STOCK USD.00001        | CUSIP: 74276L105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8988 | NS      | $282762.48    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Protagonist Therapeutics Inc.                     | PROTAGONIST THERAPEUTICS INC COMMON STOCK USD.00001    | CUSIP: 74366E102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20838 | NS      | $1819990.92   | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| RadNet Inc.                                       | RADNET INC COMMON STOCK USD.0001                       | CUSIP: 750491102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4301 | NS      | $306876.35    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Rapport Therapeutics Inc.                         | RAPPORT THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 75383L102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15921 | NS      | $483043.14    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Regeneron Pharmaceuticals Inc.                    | REGENERON PHARMACEUTICALS INC COMMON STOCK USD.001     | CUSIP: 75886F107<br>LEI: 549300RCBFWIRX3HYQ56 | Long             | EC               | CORP              | US        |      8393 | NS      | $6478304.91   | 2.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Relay Therapeutics Inc.                           | RELAY THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 75943R102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     34671 | NS      | $293316.66    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Repligen Corporation                              | REPLIGEN CORP COMMON STOCK USD.01                      | CUSIP: 759916109<br>LEI: 54930006QJ0T5AUO1E71 | Long             | EC               | CORP              | US        |      4623 | NS      | $757524.78    | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Royalty Pharma plc                                | ROYALTY PHARMA PLC COMMON STOCK                        | CUSIP: 76028H209<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     16108 | NS      | $622413.12    | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| GeneDx Holdings Corp.                             | GENEDX HOLDINGS CORP COMMON STOCK USD.0001             | CUSIP: 81663L200<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3568 | NS      | $464054.08    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Shattuck Labs Inc.                                | SHATTUCK LABS INC COMMON STOCK USD.0001                | CUSIP: 82024L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     34305 | NS      | $125213.25    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Sionna Therapeutics Inc.                          | SIONNA THERAPEUTICS INC COMMON STOCK USD.001           | CUSIP: 829401108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15281 | NS      | $628660.34    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Soleno Therapeutics Inc.                          | SOLENO THERAPEUTICS INC COMMON STOCK USD.001           | CUSIP: 834203309<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3326 | NS      | $153993.80    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                       | ABBVIE INC COMMON STOCK USD.01                         | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |     30271 | NS      | $6916620.79   | 2.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Abivax SA                                         | ABIVAX SA ADR EUR.01                                   | CUSIP: 00370M103<br>LEI: N/A                  | Long             | EC               | CORP              | FR        |      9011 | NS      | $1215178.41   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Spyre Therapeutics Inc.                           | SPYRE THERAPEUTICS INC COMMON STOCK USD.0001           | CUSIP: 00773J202<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22418 | NS      | $734413.68    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Agios Pharmaceuticals Inc.                        | AGIOS PHARMACEUTICALS INC COMMON STOCK USD.001         | CUSIP: 00847X104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8470 | NS      | $230553.40    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Arrowhead Pharmaceuticals Inc.                    | ARROWHEAD PHARMACEUTICALS INC COMMON STOCK USD.001     | CUSIP: 04280A100<br>LEI: 549300O3CSB8T7OZ3D66 | Long             | EC               | CORP              | US        |      7600 | NS      | $504564.00    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                                   | ASTRAZENECA PLC ADR                                    | CUSIP: 046353108<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |     76396 | NS      | $7023084.28   | 2.85%             |  |  |  | No            |                  1 | On Loan: No      |
| AtaiBeckley Inc.                                  | ATAIBECKLEY INC COMMON STOCK EUR.16                    | CUSIP: 04650F101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8100 | NS      | $33129.00     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Aura Biosciences Inc.                             | AURA BIOSCIENCES INC COMMON STOCK USD.00001            | CUSIP: 05153U107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10426 | NS      | $56821.70     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Axsome Therapeutics Inc.                          | AXSOME THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 05464T104<br>LEI: 549300EXGR0XLRJEIB95 | Long             | EC               | CORP              | US        |      5742 | NS      | $1048718.88   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Bicara Therapeutics Inc.                          | BICARA THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 055477103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21421 | NS      | $360515.43    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Beam Therapeutics Inc.                            | BEAM THERAPEUTICS INC COMMON STOCK USD.01              | CUSIP: 07373V105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     27477 | NS      | $761662.44    | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| BeOne Medicines Ltd                               | BEONE MEDICINES LTD ADR USD.0001                       | CUSIP: 07725L102<br>LEI: 549300JFUK6FRD5MH739 | Long             | EC               | CORP              | US        |      1932 | NS      | $586960.92    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Caris Life Sciences Inc.                          | CARIS LIFE SCIENCES INC COMMON STOCK USD.001           | CUSIP: 142152107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22632 | NS      | $610611.36    | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Celcuity Inc.                                     | CELCUITY INC COMMON STOCK USD.001                      | CUSIP: 15102K100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12901 | NS      | $1286745.74   | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Celldex Therapeutics Inc.                         | CELLDEX THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 15117B202<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11227 | NS      | $304925.32    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Centene Corporation                               | CENTENE CORP COMMON STOCK USD.001                      | CUSIP: 15135B101<br>LEI: 549300Z7JJ4TQSQGT333 | Long             | EC               | CORP              | US        |     13214 | NS      | $543756.10    | 0.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Disc Medicine Inc.                                | DISC MEDICINE INC COMMON STOCK USD.0001                | CUSIP: 254604101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9263 | NS      | $735574.83    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Edwards Lifesciences Corporation                  | EDWARDS LIFESCIENCES CORP COMMON STOCK USD1.0          | CUSIP: 28176E108<br>LEI: YA13X31F3V31L8TMPR58 | Long             | EC               | CORP              | US        |     12478 | NS      | $1063749.50   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Elanco Animal Health Incorporated                 | ELANCO ANIMAL HEALTH INC COMMON STOCK                  | CUSIP: 28414H103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9000 | NS      | $203670.00    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Climb Bio Inc.                                    | CLIMB BIO INC COMMON STOCK                             | CUSIP: 28658R106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     34800 | NS      | $139200.00    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Insmed Incorporated                               | INSMED INC COMMON STOCK USD.01                         | CUSIP: 457669307<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     27085 | NS      | $4713873.40   | 1.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Inspire Medical Systems Inc.                      | INSPIRE MEDICAL SYSTEMS INC COMMON STOCK USD.001       | CUSIP: 457730109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3200 | NS      | $295136.00    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                           | INTUITIVE SURGICAL INC COMMON STOCK USD.001            | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |     19519 | NS      | $11054780.84  | 4.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Monopar Therapeutics Inc.                         | MONOPAR THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 61023L207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |       364 | NS      | $23769.20     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Replimune Group Inc.                              | REPLIMUNE GROUP INC COMMON STOCK USD.001               | CUSIP: 76029N106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     41148 | NS      | $399958.56    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| ROWE T PRICE GOV RES FD 20340919 00000.0000000000 | ROWE T PRICE GOV RES FD 20340919 00000.0000000000      | CUSIP: 76105Y109<br>LEI: N/A                  | Long             | STIV             | RF                | US        |    367787 | NS      | $367787.28    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc.                         | REVOLUTION MEDICINES INC COMMON STOCK                  | CUSIP: 76155X100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     30724 | NS      | $2447166.60   | 0.99%             |  |  |  | No            |                  1 | On Loan: No      |
| MediWound Ltd                                     | MEDIWOUND LTD COMMON STOCK ILS.01                      | CUSIP: M68830112<br>LEI: N/A                  | Long             | EC               | CORP              | IL        |      3687 | NS      | $68062.02     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Alignment Healthcare Inc.                         | ALIGNMENT HEALTHCARE INC COMMON STOCK USD.001          | CUSIP: 01625V104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     34030 | NS      | $672092.50    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Allogene Therapeutics Inc.                        | ALLOGENE THERAPEUTICS INC COMMON STOCK USD.001         | CUSIP: 019770106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     81376 | NS      | $111485.12    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Alnylam Pharmaceuticals Inc.                      | ALNYLAM PHARMACEUTICALS INC COMMON STOCK USD.01        | CUSIP: 02043Q107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14420 | NS      | $5734113.00   | 2.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Cencora Inc.                                      | CENCORA INC COMMON STOCK USD.01                        | CUSIP: 03073E105<br>LEI: AI8GXW8LG5WK7E9UD086 | Long             | EC               | CORP              | US        |      4589 | NS      | $1549934.75   | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Annexon Inc.                                      | ANNEXON INC COMMON STOCK                               | CUSIP: 03589W102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22109 | NS      | $110987.18    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Elevance Health Inc.                              | ELEVANCE HEALTH INC COMMON STOCK USD.01                | CUSIP: 036752103<br>LEI: 8MYN82XMYQH89CTMTH67 | Long             | EC               | CORP              | US        |      6367 | NS      | $2231951.85   | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Apogee Therapeutics Inc.                          | APOGEE THERAPEUTICS INC COMMON STOCK USD.00001         | CUSIP: 03770N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14786 | NS      | $1116047.28   | 0.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcellx Inc.                                      | ARCELLX INC COMMON STOCK USD.001                       | CUSIP: 03940C100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9119 | NS      | $594558.80    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcutis Biotherapeutics Inc.                      | ARCUTIS BIOTHERAPEUTICS INC COMMON STOCK USD.0001      | CUSIP: 03969K108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12700 | NS      | $368808.00    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| argenx SE                                         | ARGENX SE ADR                                          | CUSIP: 04016X101<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |     11533 | NS      | $9698676.35   | 3.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                               | ASCENDIS PHARMA A/S ADR DKK1.0                         | CUSIP: 04351P101<br>LEI: N/A                  | Long             | EC               | CORP              | DK        |     15020 | NS      | $3202864.80   | 1.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Alkermes Public Limited Company                   | ALKERMES PLC COMMON STOCK USD.01                       | CUSIP: G01767105<br>LEI: 549300YHRMYLL071E243 | Long             | EC               | CORP              | IE        |      8455 | NS      | $236570.90    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Biohaven Ltd.                                     | BIOHAVEN LTD COMMON STOCK                              | CUSIP: G1110E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22549 | NS      | $254578.21    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Kestra Medical Technologies Ltd                   | KESTRA MEDICAL TECHNOLOGIES LT COMMON STOCK USD100.0   | CUSIP: G52441105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18799 | NS      | $498549.48    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Roivant Sciences Ltd.                             | ROIVANT SCIENCES LTD COMMON STOCK USD.0000001          | CUSIP: G76279101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     72989 | NS      | $1583861.30   | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Beta Bionics Inc.                                 | BETA BIONICS INC COMMON STOCK USD.0001                 | CUSIP: 08659B102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8425 | NS      | $256709.75    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Bicycle Therapeutics plc                          | BICYCLE THERAPEUTICS PLC ADR USD.01                    | CUSIP: 088786108<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |     25602 | NS      | $181262.16    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| BillionToOne Inc.                                 | BILLIONTOONE INC COMMON STOCK USD.00001                | CUSIP: 090168105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2903 | NS      | $237581.52    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Bio-Rad Laboratories Inc.                         | BIO-RAD LABORATORIES INC COMMON STOCK USD.0001         | CUSIP: 090572207<br>LEI: 549300ZC1GPV35XYDK53 | Long             | EC               | CORP              | US        |      1144 | NS      | $346620.56    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Bio-Techne Corporation                            | BIO-TECHNE CORP COMMON STOCK USD.01                    | CUSIP: 09073M104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8921 | NS      | $524644.01    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation                     | BOSTON SCIENTIFIC CORP COMMON STOCK USD.01             | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |     41953 | NS      | $4000218.55   | 1.62%             |  |  |  | No            |                  1 | On Loan: No      |
| BridgeBio Oncology Therapeutics Inc.              | BRIDGEBIO ONCOLOGY THERAPEUTIC COMMON STOCK USD.0001   | CUSIP: 107924102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14485 | NS      | $181352.20    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| BridgeBio Pharma Inc.                             | BRIDGEBIO PHARMA INC COMMON STOCK                      | CUSIP: 10806X102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     19233 | NS      | $1471132.17   | 0.60%             |  |  |  | No            |                  1 | On Loan: No      |
| BrightSpring Health Services Inc.                 | BRIGHTSPRING HEALTH SERVICES I COMMON STOCK USD100.0   | CUSIP: 10950A106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     54101 | NS      | $2026082.45   | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cigna Group                                   | CIGNA GROUP/THE COMMON STOCK USD.01                    | CUSIP: 125523100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7243 | NS      | $1993490.89   | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| CVS Health Corporation                            | CVS HEALTH CORP COMMON STOCK USD.01                    | CUSIP: 126650100<br>LEI: 549300EJG376EN5NQE29 | Long             | EC               | CORP              | US        |     27194 | NS      | $2158115.84   | 0.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Cabaletta Bio Inc.                                | CABALETTA BIO INC COMMON STOCK USD.00001               | CUSIP: 12674W109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2887 | NS      | $6322.53      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Cardinal Health Inc.                              | CARDINAL HEALTH INC COMMON STOCK                       | CUSIP: 14149Y108<br>LEI: CCU46N3GJMF4OK4N7U60 | Long             | EC               | CORP              | US        |      7039 | NS      | $1446514.50   | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Centessa Pharmaceuticals plc                      | CENTESSA PHARMACEUTICALS PLC ADR USD.0001              | CUSIP: 152309100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     49526 | NS      | $1238645.26   | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| CG Oncology Inc.                                  | CG ONCOLOGY INC COMMON STOCK                           | CUSIP: 156944100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     24016 | NS      | $997144.32    | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Cogent Biosciences Inc.                           | COGENT BIOSCIENCES INC COMMON STOCK USD.001            | CUSIP: 19240Q201<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7384 | NS      | $262279.68    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Crinetics Pharmaceuticals Inc.                    | CRINETICS PHARMACEUTICALS INC COMMON STOCK USD.001     | CUSIP: 22663K107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     25383 | NS      | $1181578.65   | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Cytokinetics Incorporated                         | CYTOKINETICS INC COMMON STOCK USD.001                  | CUSIP: 23282W605<br>LEI: 549300BE5DY1HW7IHA64 | Long             | EC               | CORP              | US        |     19157 | NS      | $1217235.78   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                               | DANAHER CORP COMMON STOCK USD.01                       | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |     29560 | NS      | $6766875.20   | 2.75%             |  |  |  | No            |                  1 | On Loan: No      |
| Denali Therapeutics Inc.                          | DENALI THERAPEUTICS INC COMMON STOCK USD.01            | CUSIP: 24823R105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26925 | NS      | $444531.75    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Dianthus Therapeutics Inc.                        | DIANTHUS THERAPEUTICS INC COMMON STOCK USD.001         | CUSIP: 252828108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4502 | NS      | $185527.42    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Encompass Health Corporation                      | ENCOMPASS HEALTH CORP COMMON STOCK USD.01              | CUSIP: 29261A100<br>LEI: 549300FVRPM9DRQZRG64 | Long             | EC               | CORP              | US        |      3960 | NS      | $420314.40    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Entrada Therapeutics Inc.                         | ENTRADA THERAPEUTICS INC COMMON STOCK USD.0001         | CUSIP: 29384C108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11625 | NS      | $119505.00    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Evommune Inc.                                     | EVOMMUNE INC COMMON STOCK USD.0001                     | CUSIP: 30054Y107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5551 | NS      | $95033.12     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Generation Bio Co.                                | GENERATION BIO CO COMMON STOCK USD.0001                | CUSIP: 37148K209<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3769 | NS      | $21407.92     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Gilead Sciences Inc.                              | GILEAD SCIENCES INC COMMON STOCK USD.001               | CUSIP: 375558103<br>LEI: 549300WTZWR07K8MNV44 | Long             | EC               | CORP              | US        |     44553 | NS      | $5468435.22   | 2.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Ginkgo Bioworks Holdings Inc.                     | GINKGO BIOWORKS HOLDINGS INC COMMON STOCK USD.0001     | CUSIP: 37611X209<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1971 | NS      | $16379.01     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Crescent Biopharma Inc.                           | CRESCENT BIOPHARMA INC COMMON STOCK USD.001            | CUSIP: 38000Q201<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5959 | NS      | $70673.74     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Guardant Health Inc.                              | GUARDANT HEALTH INC COMMON STOCK USD.00001             | CUSIP: 40131M109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     23103 | NS      | $2359740.42   | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| HCA Healthcare Inc.                               | HCA HEALTHCARE INC COMMON STOCK USD.01                 | CUSIP: 40412C101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4931 | NS      | $2302086.66   | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Heartflow Inc.                                    | HEARTFLOW INC COMMON STOCK USD.001                     | CUSIP: 42238D107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9934 | NS      | $289576.10    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Hims and Hers Health Inc.                         | HIMS and HERS HEALTH INC COMMON STOCK USD.0001         | CUSIP: 433000106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2300 | NS      | $74681.00     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEAYA Biosciences Inc.                           | IDEAYA BIOSCIENCES INC COMMON STOCK                    | CUSIP: 45166A102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8167 | NS      | $282333.19    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEXX Laboratories Inc.                           | IDEXX LABORATORIES INC COMMON STOCK USD.1              | CUSIP: 45168D104<br>LEI: OGMTXK0LUU1HKV2P0J84 | Long             | EC               | CORP              | US        |      2828 | NS      | $1913226.84   | 0.78%             |  |  |  | No            |                  1 | On Loan: No      |
| Immuneering Corporation                           | IMMUNEERING CORP COMMON STOCK USD.001                  | CUSIP: 45254E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17357 | NS      | $114209.06    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunome Inc.                                     | IMMUNOME INC COMMON STOCK USD.0001                     | CUSIP: 45257U108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     50101 | NS      | $1076169.48   | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunocore Holdings plc                           | IMMUNOCORE HOLDINGS PLC ADR GBP.0001                   | CUSIP: 45258D105<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |     17786 | NS      | $617352.06    | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunocore Holdings plc                           | IMMUNOCORE HOLDINGS PLC SR UNSECURED 02/30 2.5         | CUSIP: 45258DAB1<br>LEI: N/A                  | Long             | DBT              | CORP              | GB        |    277000 | PA      | $250338.75    | 0.10%             | 2030-02-01      | Fixed         | 2.50%                 | No            |                  2 | On Loan: No      |
| Immunovant Inc.                                   | IMMUNOVANT INC COMMON STOCK USD.0001                   | CUSIP: 45258J102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10631 | NS      | $270240.02    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Incyte Corporation                                | INCYTE CORP COMMON STOCK USD.001                       | CUSIP: 45337C102<br>LEI: 549300Z4WN6JVZ3T4680 | Long             | EC               | CORP              | US        |     13022 | NS      | $1286182.94   | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Medline Inc.                                      | MEDLINE INC COMMON STOCK USD.0001                      | CUSIP: 58507V107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7732 | NS      | $324744.00    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Merck and Co. Inc.                                | MERCK and CO INC COMMON STOCK USD.5                    | CUSIP: 58933Y105<br>LEI: 4YV9Y5M8S0BRK1RP0397 | Long             | EC               | CORP              | US        |     44300 | NS      | $4663018.00   | 1.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Mirum Pharmaceuticals Inc.                        | MIRUM PHARMACEUTICALS INC COMMON STOCK                 | CUSIP: 604749101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3100 | NS      | $244869.00    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Molina Healthcare Inc.                            | MOLINA HEALTHCARE INC COMMON STOCK USD.001             | CUSIP: 60855R100<br>LEI: 549300NQQCEQ46YHZ591 | Long             | EC               | CORP              | US        |      2454 | NS      | $425867.16    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Monte Rosa Therapeutics Inc.                      | MONTE ROSA THERAPEUTICS INC COMMON STOCK USD.0001      | CUSIP: 61225M102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     31809 | NS      | $498765.12    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| MoonLake Immunotherapeutics                       | MOONLAKE IMMUNOTHERAPEUTICS COMMON STOCK USD.0001      | CUSIP: 61559X104<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |     18575 | NS      | $244818.50    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Chugai Pharmaceutical Co. Ltd.                    | CHUGAI PHARMACEUTICAL CO LTD COMMON STOCK              | CUSIP: 619640006<br>LEI: N/A                  | Long             | EC               | CORP              | JP        |     23400 | NS      | $1227600.48   | 0.50%             |  |  |  | No            |                  2 | On Loan: No      |
| Natera Inc.                                       | NATERA INC COMMON STOCK                                | CUSIP: 632307104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12096 | NS      | $2771072.64   | 1.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Novo Nordisk A/S                                  | NOVO NORDISK A/S ADR                                   | CUSIP: 670100205<br>LEI: 549300DAQ1CVT6CXN342 | Long             | EC               | CORP              | DK        |     28400 | NS      | $1444992.00   | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvalent Inc.                                     | NUVALENT INC COMMON STOCK USD.0001                     | CUSIP: 670703107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12356 | NS      | $1242890.04   | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Ionis Pharmaceuticals Inc.                        | IONIS PHARMACEUTICALS INC COMMON STOCK USD.001         | CUSIP: 462222100<br>LEI: 549300SI4ZGLG0BLUZ92 | Long             | EC               | CORP              | US        |     22738 | NS      | $1798803.18   | 0.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Iovance Biotherapeutics Inc.                      | IOVANCE BIOTHERAPEUTICS INC COMMON STOCK USD.000041666 | CUSIP: 462260100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     31687 | NS      | $86505.51     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Kodiak Sciences Inc.                              | KODIAK SCIENCES INC COMMON STOCK USD.0001              | CUSIP: 50015M109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17028 | NS      | $476102.88    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Krystal Biotech Inc.                              | KRYSTAL BIOTECH INC COMMON STOCK USD.00001             | CUSIP: 501147102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7113 | NS      | $1753639.02   | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Kymera Therapeutics Inc.                          | KYMERA THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 501575104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26842 | NS      | $2088576.02   | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Kyverna Therapeutics Inc.                         | KYVERNA THERAPEUTICS INC COMMON STOCK USD.00001        | CUSIP: 501976104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     32113 | NS      | $301862.20    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| LENZ Therapeutics Inc.                            | LENZ THERAPEUTICS INC COMMON STOCK USD.00001           | CUSIP: 52635N103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3800 | NS      | $60800.00     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly and Company                             | ELI LILLY and CO COMMON STOCK                          | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |     28314 | NS      | $30428489.52  | 12.36%            |  |  |  | No            |                  1 | On Loan: No      |
| MBX Biosciences Inc.                              | MBX BIOSCIENCES INC COMMON STOCK USD.0001              | CUSIP: 55287L101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14724 | NS      | $464394.96    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Madrigal Pharmaceuticals Inc.                     | MADRIGAL PHARMACEUTICALS INC COMMON STOCK USD.0001     | CUSIP: 558868105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1506 | NS      | $877004.04    | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| U C B SA                                          | UCB SA COMMON STOCK                                    | CUSIP: 559699905<br>LEI: N/A                  | Long             | EC               | CORP              | BE        |       329 | NS      | $91668.08     | 0.04%             |  |  |  | No            |                  2 | On Loan: No      |
| McKesson Corporation                              | MCKESSON CORP COMMON STOCK USD.01                      | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |       730 | NS      | $598811.70    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Sartorius Aktiengesellschaft                      | SARTORIUS AG PREFERENCE                                | CUSIP: 584332902<br>LEI: N/A                  | Long             | EP               | CORP              | DE        |      2210 | NS      | $636401.07    | 0.26%             |  |  |  | No            |                  2 | On Loan: No      |
| Wave Life Sciences Ltd.                           | WAVE LIFE SCIENCES LTD COMMON STOCK                    | CUSIP: Y95308105<br>LEI: 54930070006GPKFYZ813 | Long             | EC               | CORP              | SG        |     37722 | NS      | $641274.00    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc.                         | REVOLUTION MEDICINES INC WARRANTS CALL EXP 17DEC26     | CUSIP: 76155X118<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4502 | NS      | $4231.43      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Rhythm Pharmaceuticals Inc.                       | RHYTHM PHARMACEUTICALS INC COMMON STOCK                | CUSIP: 76243J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7230 | NS      | $773899.20    | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Rocket Pharmaceuticals Inc.                       | ROCKET PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 77313F106<br>LEI: 549300R7VV82YUIN4Y93 | Long             | EC               | CORP              | US        |     23535 | NS      | $82607.85     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| State Street Bank and Trust Company               | SCC GOVERMENT MM GVXX 20301231 00001.0000000000        | CUSIP: 7839989D1<br>LEI: 571474TGEMMWANRLN572 | Long             | STIV             | RF                | US        |    412594 | NS      | $412593.77    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Sana Biotechnology Inc.                           | SANA BIOTECHNOLOGY INC COMMON STOCK USD.0001           | CUSIP: 799566104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10300 | NS      | $41921.00     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Scholar Rock Holding Corporation                  | SCHOLAR ROCK HOLDING CORP COMMON STOCK USD.001         | CUSIP: 80706P103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     37255 | NS      | $1641082.75   | 0.67%             |  |  |  | No            |                  1 | On Loan: No      |
| GeneDx Holdings Corp.                             | GENEDX HOLDINGS CORP GENEDX HOLDINGS CORP              | CUSIP: 81663L119<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4862 | NS      | $230.46       | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| IQVIA Holdings Inc.                               | IQVIA HOLDINGS INC COMMON STOCK USD.01                 | CUSIP: 46266C105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3750 | NS      | $845287.50    | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Janux Therapeutics Inc.                           | JANUX THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 47103J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4213 | NS      | $58139.40     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| UroGen Pharma Ltd.                                | UROGEN PHARMA LTD COMMON STOCK USD.01                  | CUSIP: M96088105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10800 | NS      | $252936.00    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Immatics N.V.                                     | IMMATICS NV COMMON STOCK                               | CUSIP: N44445109<br>LEI: N/A                  | Long             | EC               | CORP              | DE        |     14000 | NS      | $147000.00    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| NewAmsterdam Pharma Company N.V.                  | NEWAMSTERDAM PHARMA CO NV COMMON STOCK                 | CUSIP: N62509109<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |      2900 | NS      | $101732.00    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Pharvaris N.V.                                    | PHARVARIS NV COMMON STOCK EUR.12                       | CUSIP: N69605108<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |     17875 | NS      | $496031.25    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| uniQure N.V.                                      | UNIQURE NV COMMON STOCK EUR.05                         | CUSIP: N90064101<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |      4127 | NS      | $98759.11     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| SomaLogic Inc.                                    | SOMALOGIC INC WARRANTS CALL EXP 31AUG26                | CUSIP: 83444K113<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2050 | NS      | $92.00        | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| Stryker Corporation                               | STRYKER CORP COMMON STOCK USD.1                        | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |     24775 | NS      | $8707669.25   | 3.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Structure Therapeutics Inc.                       | STRUCTURE THERAPEUTICS INC ADR USD.0001                | CUSIP: 86366E106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     23229 | NS      | $1615576.95   | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Summit Therapeutics Inc.                          | SUMMIT THERAPEUTICS INC COMMON STOCK USD.01            | CUSIP: 86627T108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    106991 | NS      | $1871272.59   | 0.76%             |  |  |  | No            |                  1 | On Loan: No      |
| Tarsus Pharmaceuticals Inc.                       | TARSUS PHARMACEUTICALS INC COMMON STOCK USD.0001       | CUSIP: 87650L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8928 | NS      | $731024.64    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Tenet Healthcare Corporation                      | TENET HEALTHCARE CORP COMMON STOCK USD.05              | CUSIP: 88033G407<br>LEI: 0W9AIBT6U6ADJ2I8HK17 | Long             | EC               | CORP              | US        |     12583 | NS      | $2500493.76   | 1.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Terns Pharmaceuticals Inc.                        | TERNS PHARMACEUTICALS INC COMMON STOCK USD.0001        | CUSIP: 880881107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9994 | NS      | $403757.60    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Teva Pharmaceutical Industries Limited            | TEVA PHARMACEUTICAL INDUSTRIES ADR                     | CUSIP: 881624209<br>LEI: 549300BAFGM4RC74ZJ94 | Long             | EC               | CORP              | IL        |     16200 | NS      | $505602.00    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Thermo Fisher Scientific Inc.                     | THERMO FISHER SCIENTIFIC INC COMMON STOCK USD1.0       | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |     18203 | NS      | $10547728.35  | 4.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Ultragenyx Pharmaceutical Inc.                    | ULTRAGENYX PHARMACEUTICAL INC COMMON STOCK USD.001     | CUSIP: 90400D108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12318 | NS      | $283314.00    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation                   | UNITED THERAPEUTICS CORP COMMON STOCK USD.01           | CUSIP: 91307C102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4953 | NS      | $2413349.25   | 0.98%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Incorporated                   | UNITEDHEALTH GROUP INC COMMON STOCK USD.01             | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |     24695 | NS      | $8152066.45   | 3.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc.                                      | VAXCYTE INC COMMON STOCK USD.001                       | CUSIP: 92243G108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11005 | NS      | $507770.70    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Vera Therapeutics Inc.                            | VERA THERAPEUTICS INC COMMON STOCK USD.001             | CUSIP: 92337R101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     43375 | NS      | $2196510.00   | 0.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated               | VERTEX PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |      9444 | NS      | $4281531.84   | 1.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Viridian Therapeutics Inc.                        | VIRIDIAN THERAPEUTICS INC COMMON STOCK USD.01          | CUSIP: 92790C104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     16800 | NS      | $522816.00    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Voyager Therapeutics Inc.                         | VOYAGER THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 92915B106<br>LEI: 54930082S4XVR0ZWCC74 | Long             | EC               | CORP              | US        |     15964 | NS      | $62738.52     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Waters Corporation                                | WATERS CORP COMMON STOCK USD.01                        | CUSIP: 941848103<br>LEI: 5T547R1474YC9HOD8Q74 | Long             | EC               | CORP              | US        |      1800 | NS      | $683694.00    | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Xencor Inc.                                       | XENCOR INC COMMON STOCK USD.01                         | CUSIP: 98401F105<br>LEI: 549300V5IF65437JKG30 | Long             | EC               | CORP              | US        |     13179 | NS      | $201770.49    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Xenon Pharmaceuticals Inc.                        | XENON PHARMACEUTICALS INC COMMON STOCK                 | CUSIP: 98420N105<br>LEI: N/A                  | Long             | EC               | CORP              | CA        |      2900 | NS      | $129978.00    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Zai Lab Limited                                   | ZAI LAB LTD ADR USD.00006                              | CUSIP: 98887Q104<br>LEI: N/A                  | Long             | EC               | CORP              | CN        |     12926 | NS      | $228014.64    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Zoetis Inc.                                       | ZOETIS INC COMMON STOCK USD.01                         | CUSIP: 98978V103<br>LEI: 549300HD9Q1LOC9KLJ48 | Long             | EC               | CORP              | US        |      3100 | NS      | $390042.00    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Saluda Medical Inc.                               | SALUDA MEDICAL INC CDI                                 | CUSIP: BRC373908<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     79512 | NS      | $75613.83     | 0.03%             |  |  |  | No            |                  2 | On Loan: No      |
| BeOne Medicines Ltd                               | BEONE MEDICINES LTD COMMON STOCK USD.0001              | CUSIP: BVDKGC900<br>LEI: 549300JFUK6FRD5MH739 | Long             | EC               | CORP              | US        |    140298 | NS      | $3229137.43   | 1.31%             |  |  |  | No            |                  2 | On Loan: No      |
| Sensorion                                         | SENSORION SA COMMON STOCK EUR.1                        | CUSIP: BWSW4N905<br>LEI: N/A                  | Long             | EC               | CORP              | FR        |    182870 | NS      | $72100.19     | 0.03%             |  |  |  | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2026-01-29

**Name of Applicant:** John Hancock Variable Insurance Trust

**Signature:** /s/Salvatore Schiavone

**Name of Signer:** Salvatore Schiavone

**Title:** Treasurer