# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0000277751-26-000158
**Filing Date:** 2026-5
**Character Count:** 35049
**Document Hash:** b71d51f67d43961ddf13fc792a590ede
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0000277751-26-000158.hdr.sgml**: 20260529

**ACCESSION NUMBER**: 0000277751-26-000158

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20260331

**FILED AS OF DATE**: 20260529

**DATE AS OF CHANGE**: 20260529

**PERIOD START**: 20260930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 261042086

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Research Fund (Series ID: S000010470)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028926 | Class T      | JAMRX           |
| C000077669 | Class A      | JRAAX           |
| C000077670 | Class C      | JRACX           |
| C000077671 | Class I      | JRAIX           |
| C000077672 | Class S      | JRASX           |
| C000083517 | Class D      | JNRFX           |
| C000114872 | Class N      | JRANX           |
| C000177053 | Class R      | JRARX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Research Fund

- **b. EDGAR series identifier (if any):** S000010470

- **c. LEI of Series:** TO5UP72VAT80SBB47C78

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2026-09-30

- **b. Date as of which information is reported:** 2026-03-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $24779176548.75

**Total Liabilities:** $19620306.69

**Net Assets:** $24759556242.06

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028926 | -1.33%               | -3.83%               | -5.89%               |
| Class ID C000077669 | -1.34%               | -3.83%               | -5.90%               |
| Class ID C000077670 | -1.36%               | -3.89%               | -5.96%               |
| Class ID C000077671 | -1.33%               | -3.82%               | -5.88%               |
| Class ID C000077672 | -1.36%               | -3.84%               | -5.92%               |
| Class ID C000083517 | -1.33%               | -3.81%               | -5.88%               |
| Class ID C000114872 | -1.31%               | -3.80%               | -5.88%               |
| Class ID C000177053 | -1.38%               | -3.86%               | -5.93%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $52752624.00             | $-418815330.00                             |
| Month 2  | $-142227113.00           | $-911131885.00                             |
| Month 3  | $8170607.00              | $-1556599102.00                            |

**Designated Index Information**

- **Index Name:** Russell 1000 Growth Total Return Index

- **Index Identifier:** RUSSELL.MC.RLG

### Schedule of Portfolio Investments

| Name                                         | Title                                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)    | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Amazon.com, Inc.                             | Amazon.com, Inc. COM USD0.01                                          | CUSIP: 023135106<br>LEI: ZXTILKJKG63JELOEG630 | Long             | EC               | CORP              | US        |   5913968 | NS      | $1231702115.36 | 4.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                                   | Amgen Inc. COM USD0.0001                                              | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |    129974 | NS      | $45731351.90   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Amphenol Corporation                         | Amphenol Corporation CL'A'COM USD0.001                                | CUSIP: 032095101<br>LEI: 549300D3L3G0R4U4VT04 | Long             | EC               | CORP              | US        |   1857078 | NS      | $234641805.30  | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| Analog Devices Inc.                          | Analog Devices Inc. COM USD0.16 2/3                                   | CUSIP: 032654105<br>LEI: GYVOE5EZ4GDAVTU4CQ61 | Long             | EC               | CORP              | US        |    382154 | NS      | $121578473.56  | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Datadog Inc                                  | Datadog Inc COM USD0.00001 CL A                                       | CUSIP: 23804L103<br>LEI: 549300F6JNO0KRPO1K63 | Long             | EC               | CORP              | US        |   1726047 | NS      | $203759848.35  | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Deere & Company                              | Deere & Company COM USD1                                              | CUSIP: 244199105<br>LEI: PWFTNG3EI0Y73OXWDH08 | Long             | EC               | CORP              | US        |    240623 | NS      | $135542935.90  | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| DexCom Inc                                   | DexCom Inc COM USD0.001                                               | CUSIP: 252131107<br>LEI: 549300YSK3QDSFR5EU59 | Long             | EC               | CORP              | US        |    989693 | NS      | $62152720.40   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del                       | Liberty Media Corp Del COM LBTY ONE S C                               | CUSIP: 531229755<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |   2034644 | NS      | $172985432.88  | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Lumentum Holdings Inc.                       | Lumentum Holdings Inc. COM USD0.001                                   | CUSIP: 55024U109<br>LEI: 549300JLWRRC38DWEF52 | Long             | EC               | CORP              | US        |    128791 | NS      | $90509163.16   | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| MasterCard Incorporated                      | MasterCard Incorporated COM USD0.0001 CLASS A                         | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |    927429 | NS      | $463399174.14  | 1.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Mckesson Corp.                               | Mckesson Corp. COM USD0.01                                            | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |    138448 | NS      | $119807361.28  | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                                 | DoorDash Inc CL A                                                     | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |    705583 | NS      | $105943287.45  | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Walt Disney Company                          | Walt Disney Company COM DISNEY USD0.01                                | CUSIP: 254687106<br>LEI: 549300GZKULIZ0WOW665 | Long             | EC               | CORP              | US        |    370703 | NS      | $35728355.14   | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Johnson & Johnson                            | Johnson & Johnson COM USD1.00                                         | CUSIP: 478160104<br>LEI: 549300G0CFPGEF6X2043 | Long             | EC               | CORP              | US        |    369180 | NS      | $90242359.20   | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                    | KLA Corp. COM USD0.001                                                | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    231110 | NS      | $340288675.10  | 1.37%             |  |  |  | No            |                  1 | On Loan: No      |
| LPL Financial Holdings Inc.                  | LPL Financial Holdings Inc. COM USD0.001                              | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |    320278 | NS      | $96349230.74   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corporation                     | Lam Research Corporation COM NEW                                      | CUSIP: 512807306<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |   2129705 | NS      | $455032770.30  | 1.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Lennar Corp.                                 | Lennar Corp. COM USD0.10 CLASS A                                      | CUSIP: 526057104<br>LEI: 529900G61XVRLX5TJX09 | Long             | EC               | CORP              | US        |   1161685 | NS      | $100880725.40  | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation              | United Therapeutics Corporation COM USD0.01                           | CUSIP: 91307C102<br>LEI: 5299005C4HZL4UWROC14 | Long             | EC               | CORP              | US        |     76641 | NS      | $45446580.18   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                                  | Vaxcyte Inc COM                                                       | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |    873455 | NS      | $50756470.05   | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Meta Platforms Inc                           | Meta Platforms Inc COM USD0.000006 CL 'A'                             | CUSIP: 30303M102<br>LEI: BQ4BKCS1HXDV9HN80Z93 | Long             | EC               | CORP              | US        |   1554430 | NS      | $889336035.90  | 3.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Merck & Co., Inc                             | Merck & Co., Inc COM USD0.50                                          | CUSIP: 58933Y105<br>LEI: 4YV9Y5M8S0BRK1RP0397 | Long             | EC               | CORP              | US        |    440191 | NS      | $52950575.39   | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp.                              | Microsoft Corp. COM USD0.00000625                                     | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |   6346546 | NS      | $2349300932.82 | 9.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Micron Technology Inc.                       | Micron Technology Inc. COM USD0.10                                    | CUSIP: 595112103<br>LEI: B3DXGBC8GAIYWI2Z0172 | Long             | EC               | CORP              | US        |    503770 | NS      | $170193656.80  | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated          | Vertex Pharmaceuticals Incorporated COM USD0.01                       | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |    347564 | NS      | $155201228.56  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Visa Inc                                     | Visa Inc COM STK USD0.0001                                            | CUSIP: 92826C839<br>LEI: 549300JZ4OKEHW3DPJ59 | Long             | EC               | CORP              | US        |   1799028 | NS      | $543738222.72  | 2.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Mirum Pharmaceuticals Inc.                   | Mirum Pharmaceuticals Inc. COM USD0.0001                              | CUSIP: 604749101<br>LEI: 254900YZKR40F7F27K81 | Long             | EC               | CORP              | US        |    383566 | NS      | $35433827.08   | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Monster Beverage Corp                        | Monster Beverage Corp COM USD0.005                                    | CUSIP: 61174X109<br>LEI: 52990090AP0E7HCB6F33 | Long             | EC               | CORP              | US        |   1350122 | NS      | $97829840.12   | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Vistra Corp                                  | Vistra Corp COM USD0.01                                               | CUSIP: 92840M102<br>LEI: 549300KP43CPCUJOOG15 | Long             | EC               | CORP              | US        |    711487 | NS      | $106957840.71  | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Wayfair Inc                                  | Wayfair Inc COM USD0.001 A                                            | CUSIP: 94419L101<br>LEI: 54930027N9ZAVYFDHK53 | Long             | EC               | CORP              | US        |    641640 | NS      | $48257744.40   | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Wingstop Inc                                 | Wingstop Inc COM USD0.01                                              | CUSIP: 974155103<br>LEI: 549300SSH3F8HPIM3Q47 | Long             | EC               | CORP              | US        |     65592 | NS      | $10164792.24   | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Netflix, Inc.                                | Netflix, Inc. COM USD0.001                                            | CUSIP: 64110L106<br>LEI: 549300Y7VHGU0I7CE873 | Long             | EC               | CORP              | US        |   5355216 | NS      | $514904018.40  | 2.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Nike, Inc.                                   | Nike, Inc. CLASS'B'COM NPV                                            | CUSIP: 654106103<br>LEI: 787RXPR0UX0O0XUXPZ81 | Long             | EC               | CORP              | US        |    759596 | NS      | $40121860.72   | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| NVIDIA Corp.                                 | NVIDIA Corp. COM USD0.001                                             | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |  20633568 | NS      | $3598494259.20 | 14.53%            |  |  |  | No            |                  1 | On Loan: No      |
| O'Reilly Automotive Inc.                     | O'Reilly Automotive Inc. USD0.01                                      | CUSIP: 67103H107<br>LEI: 549300K2RLMQL149Q332 | Long             | EC               | CORP              | US        |   1526619 | NS      | $140922199.89  | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Oracle Corp.                                 | Oracle Corp. COM USD0.01                                              | CUSIP: 68389X105<br>LEI: 1Z4GXXU7ZHVWFCD8TV52 | Long             | EC               | CORP              | US        |    901902 | NS      | $132678803.22  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| The Procter & Gamble Co.                     | The Procter & Gamble Co. COM NPV                                      | CUSIP: 742718109<br>LEI: 2572IBTT8CCZW6AU4141 | Long             | EC               | CORP              | US        |   1422833 | NS      | $205513998.52  | 0.83%             |  |  |  | No            |                  1 | On Loan: No      |
| The Progressive Corporation                  | The Progressive Corporation COM USD1.00                               | CUSIP: 743315103<br>LEI: 529900TACNVLY9DCR586 | Long             | EC               | CORP              | US        |    376738 | NS      | $74684541.12   | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc                     | Revolution Medicines Inc COM                                          | CUSIP: 76155X100<br>LEI: 549300JU3X0LBCQ65892 | Long             | EC               | CORP              | US        |    502273 | NS      | $48846049.25   | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Snowflake Inc                                | Snowflake Inc COM SHS                                                 | CUSIP: 833445109<br>LEI: 254900CJNSY1K7T53Z16 | Long             | EC               | CORP              | US        |   1230582 | NS      | $185596377.24  | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| TFI International Inc                        | TFI International Inc COM                                             | CUSIP: 87241L109<br>LEI: 5493007R9W7KO7SJN678 | Long             | EC               | CORP              | US        |   1031575 | NS      | $112059992.25  | 0.45%             |  |  |  | No            |                  1 | On Loan: No      |
| The TJX Companies Inc.                       | The TJX Companies Inc. COM USD1                                       | CUSIP: 872540109<br>LEI: V167QI9I69W364E2DY52 | Long             | EC               | CORP              | US        |   1742148 | NS      | $278221035.60  | 1.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Co., Ltd. | Taiwan Semiconductor Manufacturing Co., Ltd. SPON ADS REP 5 ORD TWD10 | CUSIP: 874039100<br>LEI: 549300KB6NK5SBD14S87 | Long             |  | CORP              | US        |    462140 | NS      | $156180213.00  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Tesla Inc                                    | Tesla Inc COM USD0.001                                                | CUSIP: 88160R101<br>LEI: 54930043XZGB27CTOV49 | Long             | EC               | CORP              | US        |    430567 | NS      | $160063282.25  | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Seagate Technology Holdings PLC              | Seagate Technology Holdings PLC ORD SHS                               | CUSIP: G7997R103<br>LEI: 635400RUXIFEZSRU8X70 | Long             | EC               | CORP              | US        |    330483 | NS      | $129470020.08  | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Trane Technologies PLC                       | Trane Technologies PLC COM USD1                                       | CUSIP: G8994E103<br>LEI: 549300BURLR9SLYY2705 | Long             | EC               | CORP              | US        |    484183 | NS      | $201778423.42  | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Spotify Technology S.A.                      | Spotify Technology S.A. COM EUR0.000625                               | CUSIP: L8681T102<br>LEI: 549300B4X0JHWV0DTD60 | Long             | EC               | CORP              | US        |    420513 | NS      | $203910958.83  | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Royal Caribbean Group                        | Royal Caribbean Group COM USD0.01                                     | CUSIP: V7780T103<br>LEI: K2NEH8QNVW44JIWK7Z55 | Long             | EC               | CORP              | US        |    454355 | NS      | $125029408.90  | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                  | AbbVie Inc. COM USD0.01                                               | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |   1767538 | NS      | $384421839.62  | 1.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Alphabet Inc.                                | Alphabet Inc. CAP USD0.001 CL C                                       | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |   5853266 | NS      | $1679067884.76 | 6.78%             |  |  |  | No            |                  1 | On Loan: No      |
| Ecolab Inc.                                  | Ecolab Inc. COM USD1                                                  | CUSIP: 278865100<br>LEI: 82DYEISM090VG8LTLS26 | Long             | EC               | CORP              | US        |    486226 | NS      | $129345840.52  | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson Enterprises Inc                     | Ferguson Enterprises Inc COMMON STOCK NEW                             | CUSIP: 31488V107<br>LEI: 2138003JYQMRP3SLX189 | Long             | EC               | CORP              | US        |    453951 | NS      | $105888610.26  | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| GE Vernova Inc.                              | GE Vernova Inc. COM                                                   | CUSIP: 36828A101<br>LEI: 254900DP080RU6OK2553 | Long             | EC               | CORP              | US        |    181088 | NS      | $158071715.20  | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| GE Aerospace                                 | GE Aerospace COM NEW                                                  | CUSIP: 369604301<br>LEI: 3C7474T6CDKPR9K6YT90 | Long             | EC               | CORP              | US        |   1095793 | NS      | $310953179.61  | 1.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Hilton Worldwide Holdings Inc                | Hilton Worldwide Holdings Inc COM USD0.01                             | CUSIP: 43300A203<br>LEI: 549300HVGPK36ICB0B89 | Long             | EC               | CORP              | US        |    650240 | NS      | $197724979.20  | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Howmet Aerospace Inc                         | Howmet Aerospace Inc COM                                              | CUSIP: 443201108<br>LEI: 549300HO5WFZUT5N2T22 | Long             | EC               | CORP              | US        |   1143461 | NS      | $263522022.06  | 1.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuit Inc                                   | Intuit Inc COM USD0.01                                                | CUSIP: 461202103<br>LEI: VI90HBPH7XSFMB9E4M29 | Long             | EC               | CORP              | US        |    707600 | NS      | $305952088.00  | 1.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                      | Intuitive Surgical Inc. COM USD0.001                                  | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |    338019 | NS      | $155823378.81  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                              | Eli Lilly & Co. COM NPV                                               | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |    775283 | NS      | $713082044.91  | 2.88%             |  |  |  | No            |                  1 | On Loan: No      |
| 3M Company                                   | 3M Company COM USD0.01                                                | CUSIP: 88579Y101<br>LEI: LUZQVYP4VS22CLWDAR65 | Long             | EC               | CORP              | US        |   1411769 | NS      | $205031211.87  | 0.83%             |  |  |  | No            |                  1 | On Loan: No      |
| Eaton Corporation PLC                        | Eaton Corporation PLC COM USD0.01                                     | CUSIP: G29183103<br>LEI: 549300VDIGTMXUNT7H71 | Long             | EC               | CORP              | US        |    454371 | NS      | $162514875.57  | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Flutter Entertainment PLC                    | Flutter Entertainment PLC ORD EUR0.09 (DI)                            | CUSIP: G3643J108<br>LEI: 635400EG4YIJLJMZJ782 | Long             | EC               | CORP              | US        |    428538 | NS      | $43689449.10   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Apollo Global Management Inc                 | Apollo Global Management Inc COM                                      | CUSIP: 03769M106<br>LEI: 254900LMFT1CY9IYB476 | Long             | EC               | CORP              | US        |    796370 | NS      | $88731545.40   | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                                   | Apple Inc. COM USD0.00001                                             | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |   6440559 | NS      | $1634549468.61 | 6.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Applied Materials Inc.                       | Applied Materials Inc. COM USD0.01                                    | CUSIP: 038222105<br>LEI: 41BNNE1AFPNAZELZ6K07 | Long             | EC               | CORP              | US        |    392551 | NS      | $134170006.29  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Applovin Corp.                               | Applovin Corp. COM CL A                                               | CUSIP: 03831W108<br>LEI: 549300LLVXMUAOL3SQ07 | Long             | EC               | CORP              | US        |    454597 | NS      | $180929606.00  | 0.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Ares Management Corporation                  | Ares Management Corporation COM USD0.01 CLASS A                       | CUSIP: 03990B101<br>LEI: 529900JUV61FH7B8VW38 | Long             | EC               | CORP              | US        |    881131 | NS      | $96131392.10   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                    | Argenx SE SPON ADR EACH REP 1 ORD SHS                                 | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |     85063 | NS      | $62117255.75   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Arista Networks Inc                          | Arista Networks Inc COM SHS                                           | CUSIP: 040413205<br>LEI: 635400H1WKBLOQERUU95 | Long             | EC               | CORP              | US        |   1736297 | NS      | $213182545.66  | 0.86%             |  |  |  | No            |                  1 | On Loan: No      |
| Aurora Innovation Inc                        | Aurora Innovation Inc CLASS A COM                                     | CUSIP: 051774107<br>LEI: 984500AB2E0D9F7D9D76 | Long             | EC               | CORP              | US        |  10254057 | NS      | $42246714.84   | 0.17%             |  |  |  | No            |                  1 | On Loan: —       |
| Blackstone Inc                               | Blackstone Inc COM USD0.00001 CLASS A                                 | CUSIP: 09260D107<br>LEI: 5299004LW4QWGZUB8Y96 | Long             | EC               | CORP              | US        |   1261577 | NS      | $145068739.23  | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Boeing Co.                                   | Boeing Co. COM USD5.00                                                | CUSIP: 097023105<br>LEI: RVHJWBXLJ1RFUBSY1F30 | Long             | EC               | CORP              | US        |    805814 | NS      | $160381160.42  | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                         | Booking Holdings Inc COM USD0.008                                     | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |     56388 | NS      | $237411524.16  | 0.96%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation                | Boston Scientific Corporation COM USD0.01                             | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |   1514819 | NS      | $95054892.25   | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Bridgebio Pharma Inc                         | Bridgebio Pharma Inc COM USD0.001                                     | CUSIP: 10806X102<br>LEI: 54930028YG89VTY2J114 | Long             | EC               | CORP              | US        |    396241 | NS      | $29424856.66   | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadcom Inc                                 | Broadcom Inc COM USD0.001                                             | CUSIP: 11135F101<br>LEI: 549300WV6GIDOZJTV909 | Long             | EC               | CORP              | US        |   4654791 | NS      | $1440704362.41 | 5.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Cadence Design Systems, Inc.                 | Cadence Design Systems, Inc. COM USD0.01                              | CUSIP: 127387108<br>LEI: GCT7RXJOGLXPV0NXZY22 | Long             | EC               | CORP              | US        |    634821 | NS      | $176397711.27  | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Capital One Financial Corp.                  | Capital One Financial Corp. COM USD0.01                               | CUSIP: 14040H105<br>LEI: ZUE8T73ROZOF6FLBAR73 | Long             | EC               | CORP              | US        |    421091 | NS      | $76819631.13   | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Brands, Inc.                   | Constellation Brands, Inc. COM USD0.01 CLASS A                        | CUSIP: 21036P108<br>LEI: 5493005GKEG8QCVY7037 | Long             | EC               | CORP              | US        |    416343 | NS      | $62451450.00   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Gallagher Aj &Co                             | Gallagher Aj &Co COM USD1.00                                          | CUSIP: 363576109<br>LEI: 54930049QLLMPART6V29 | Long             | EC               | CORP              | US        |    378528 | NS      | $81981594.24   | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlas Energy Solutions Inc.                  | Atlas Energy Solutions Inc. COM NEW                                   | CUSIP: 642045108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2397418 | NS      | $31454124.16   | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                          | Royal Bank of Canada                                                  | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |    401520 | PA      | $401520.00     | 0.00%             | 2026-04-01      | Fixed         | 3.65%                 | No            |                  2 | On Loan: No      |
| Janus Henderson Cash Collateral Fund LLC     | Janus Henderson Cash Collateral Fund LLC                              | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |   1761280 | NS      | $1761280.00    | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2026-05-15

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER