# EDGAR Filing Document

**Accession Number:** 0000756913
**File Stem:** 0001145549-25-056562
**Filing Date:** 2025-8
**Character Count:** 57618
**Document Hash:** 10e5f895c062ecdba0ffed5ed91a630b
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001145549-25-056562.hdr.sgml**: 20250829

**ACCESSION NUMBER**: 0001145549-25-056562

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250630

**FILED AS OF DATE**: 20250829

**DATE AS OF CHANGE**: 20250828

**PERIOD START**: 20251231

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JOHN HANCOCK VARIABLE INSURANCE TRUST
- **CENTRAL INDEX KEY:** 0000756913

**ORGANIZATION NAME:**
- **EIN:** 000000000
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 0630

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-04146
- **FILM NUMBER:** 251275385

**BUSINESS ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116
- **BUSINESS PHONE:** 617-663-3000

**MAIL ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JOHN HANCOCK TRUST
- **DATE OF NAME CHANGE:** 20050124

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** MANUFACTURERS INVESTMENT TRUST
- **DATE OF NAME CHANGE:** 19971022

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** NASL SERIES FUND INC
- **DATE OF NAME CHANGE:** 19881030

## Series and Classes Contracts Data

### Health Sciences Trust (Series ID: S000008239)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000022442 | NAV          | JAFMX           |
| C000022443 | Series I     | JEHSX           |
| C000022444 | Series II    | JAFKX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** John Hancock Variable Insurance Trust

- **b. Investment Company Act file number:** 811-04146

- **c. CIK number of Registrant:** 0000756913

- **d. LEI of Registrant:** Z5DUWKEPW78QKD87GA06

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 200 Berkeley Street

  - **City:** Boston

  - **State:** MA

  - **Foreign country:** US

  - **Zip / Postal Code:** 02116

  - **Telephone number:** 617-543-9634

**Item A.2. Information about the Series.**

- **a. Name of Series:** Health Sciences Trust

- **b. EDGAR series identifier (if any):** S000008239

- **c. LEI of Series:** K5HWWGQWUOYDF5BZA919

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-12-31

- **b. Date as of which information is reported:** 2025-06-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $212733297.12

**Total Liabilities:** $56708.03

**Net Assets:** $212676589.09

**Cash Not Reported:** $14362.53

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000022442 | 0.04%                | -6.22%               | 4.11%                |
| Class ID C000022443 | 0.05%                | -6.23%               | 4.14%                |
| Class ID C000022444 | 0.05%                | -6.28%               | 4.09%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $-397794.46              | $597789.15                                 |
| Month 2  | $-1863270.24             | $-12082909.11                              |
| Month 3  | $1559362.99              | $6932512.83                                |

### Schedule of Portfolio Investments

| Name                                              | Title                                                  | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Chugai Pharmaceutical Co. Ltd.                    | CHUGAI PHARMACEUTICAL CO LTD COMMON STOCK              | CUSIP: 619640006<br>LEI: N/A                  | Long             | EC               | CORP              | JP        |     45800 | NS      | $2391576.15   | 1.12%             |  |  |  | No            |                  2 | On Loan: No      |
| Natera Inc.                                       | NATERA INC COMMON STOCK                                | CUSIP: 632307104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11288 | NS      | $1906994.72   | 0.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Neurocrine Biosciences Inc.                       | NEUROCRINE BIOSCIENCES INC COMMON STOCK USD.001        | CUSIP: 64125C109<br>LEI: 549300FECER0XBN49756 | Long             | EC               | CORP              | US        |     12357 | NS      | $1553151.33   | 0.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvalent Inc.                                     | NUVALENT INC COMMON STOCK USD.0001                     | CUSIP: 670703107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7847 | NS      | $598726.10    | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Orchestra Biomed Holdings Inc.                    | ORCHESTRA BIOMED HOLDINGS INC COMMON STOCK USD.0001    | CUSIP: 68572M106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13354 | NS      | $35788.72     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| ORIC Pharmaceuticals Inc.                         | ORIC PHARMACEUTICALS INC COMMON STOCK USD.0001         | CUSIP: 68622P109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     25630 | NS      | $260144.50    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Oruka Therapeutics Inc.                           | ORUKA THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 687604108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13401 | NS      | $150225.21    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Oscar Health Inc.                                 | OSCAR HEALTH INC COMMON STOCK USD.00001                | CUSIP: 687793109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12852 | NS      | $275546.88    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Penumbra Inc.                                     | PENUMBRA INC COMMON STOCK USD.001                      | CUSIP: 70975L107<br>LEI: 549300RGE06P3ECANU72 | Long             | EC               | CORP              | US        |     12405 | NS      | $3183495.15   | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Revvity Inc.                                      | REVVITY INC COMMON STOCK USD1.0                        | CUSIP: 714046109<br>LEI: 549300IKL1SDPFI7N655 | Long             | EC               | CORP              | US        |      8838 | NS      | $854811.36    | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Protagonist Therapeutics Inc.                     | PROTAGONIST THERAPEUTICS INC COMMON STOCK USD.00001    | CUSIP: 74366E102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15241 | NS      | $842370.07    | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Quest Diagnostics Incorporated                    | QUEST DIAGNOSTICS INC COMMON STOCK USD.01              | CUSIP: 74834L100<br>LEI: 8MCWUBXQ0WE04KMXBX50 | Long             | EC               | CORP              | US        |      8237 | NS      | $1479612.31   | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Rapport Therapeutics Inc.                         | RAPPORT THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 75383L102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22372 | NS      | $254369.64    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Regeneron Pharmaceuticals Inc.                    | REGENERON PHARMACEUTICALS INC COMMON STOCK USD.001     | CUSIP: 75886F107<br>LEI: 549300RCBFWIRX3HYQ56 | Long             | EC               | CORP              | US        |      4418 | NS      | $2319450.00   | 1.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Relay Therapeutics Inc.                           | RELAY THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 75943R102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     32881 | NS      | $113768.26    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Repligen Corporation                              | REPLIGEN CORP COMMON STOCK USD.01                      | CUSIP: 759916109<br>LEI: 54930006QJ0T5AUO1E71 | Long             | EC               | CORP              | US        |      7782 | NS      | $967925.16    | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Replimune Group Inc.                              | REPLIMUNE GROUP INC COMMON STOCK USD.001               | CUSIP: 76029N106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     47161 | NS      | $438125.69    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| ROWE T PRICE GOV RES FD 20340919 00000.0000000000 | ROWE T PRICE GOV RES FD 20340919 00000.0000000000      | CUSIP: 76105Y109<br>LEI: N/A                  | Long             | STIV             | RF                | US        |   1944821 | NS      | $1944821.06   | 0.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc.                         | REVOLUTION MEDICINES INC COMMON STOCK                  | CUSIP: 76155X100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     28063 | NS      | $1032437.77   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc.                         | REVOLUTION MEDICINES INC WARRANTS CALL EXP 17DEC26     | CUSIP: 76155X118<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4502 | NS      | $1215.54      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Rhythm Pharmaceuticals Inc.                       | RHYTHM PHARMACEUTICALS INC COMMON STOCK                | CUSIP: 76243J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7936 | NS      | $501475.84    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Apogee Therapeutics Inc.                          | APOGEE THERAPEUTICS INC COMMON STOCK USD.00001         | CUSIP: 03770N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13302 | NS      | $577705.86    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Rocket Pharmaceuticals Inc.                       | ROCKET PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 77313F106<br>LEI: 549300R7VV82YUIN4Y93 | Long             | EC               | CORP              | US        |     23535 | NS      | $57660.75     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| State Street Bank and Trust Company               | SCC GOVERMENT MM GVXX 20301231 00001.0000000000        | CUSIP: 7839989D1<br>LEI: 571474TGEMMWANRLN572 | Long             | STIV             | RF                | US        |    500000 | NS      | $499999.80    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcellx Inc.                                      | ARCELLX INC COMMON STOCK USD.001                       | CUSIP: 03940C100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9687 | NS      | $637888.95    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| argenx SE                                         | ARGENX SE ADR                                          | CUSIP: 04016X101<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |     13002 | NS      | $7166962.44   | 3.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Sana Biotechnology Inc.                           | SANA BIOTECHNOLOGY INC COMMON STOCK USD.0001           | CUSIP: 799566104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     54773 | NS      | $149530.29    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Sanofi                                            | SANOFI SA ADR                                          | CUSIP: 80105N105<br>LEI: 549300E9PC51EN656011 | Long             | EC               | CORP              | FR        |     26965 | NS      | $1302679.15   | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Arvinas Inc.                                      | ARVINAS INC COMMON STOCK USD.001                       | CUSIP: 04335A105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4849 | NS      | $35688.64     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Celldex Therapeutics Inc.                         | CELLDEX THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 15117B202<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17861 | NS      | $363471.35    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Scholar Rock Holding Corporation                  | SCHOLAR ROCK HOLDING CORP COMMON STOCK USD.001         | CUSIP: 80706P103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     39504 | NS      | $1399231.68   | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| GeneDx Holdings Corp.                             | GENEDX HOLDINGS CORP GENEDX HOLDINGS CORP              | CUSIP: 81663L119<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4862 | NS      | $320.89       | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Centessa Pharmaceuticals plc                      | CENTESSA PHARMACEUTICALS PLC ADR USD.0001              | CUSIP: 152309100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     69050 | NS      | $907317.00    | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| CG Oncology Inc.                                  | CG ONCOLOGY INC COMMON STOCK                           | CUSIP: 156944100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26067 | NS      | $677742.00    | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Sionna Therapeutics Inc.                          | SIONNA THERAPEUTICS INC COMMON STOCK USD.001           | CUSIP: 829401108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7726 | NS      | $134046.10    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Soleno Therapeutics Inc.                          | SOLENO THERAPEUTICS INC COMMON STOCK USD.001           | CUSIP: 834203309<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12538 | NS      | $1050433.64   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cooper Companies Inc.                         | COOPER COS INC/THE COMMON STOCK USD.1                  | CUSIP: 216648501<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |     21033 | NS      | $1496708.28   | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunome Inc.                                     | IMMUNOME INC COMMON STOCK USD.0001                     | CUSIP: 45257U108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     56594 | NS      | $526324.20    | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Tarsus Pharmaceuticals Inc.                       | TARSUS PHARMACEUTICALS INC COMMON STOCK USD.0001       | CUSIP: 87650L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7654 | NS      | $310063.54    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Thermo Fisher Scientific Inc.                     | THERMO FISHER SCIENTIFIC INC COMMON STOCK USD1.0       | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |     16290 | NS      | $6604943.40   | 3.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunocore Holdings plc                           | IMMUNOCORE HOLDINGS PLC ADR GBP.0001                   | CUSIP: 45258D105<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |     18135 | NS      | $569076.30    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Insmed Incorporated                               | INSMED INC COMMON STOCK USD.01                         | CUSIP: 457669307<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     46148 | NS      | $4644334.72   | 2.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Ultragenyx Pharmaceutical Inc.                    | ULTRAGENYX PHARMACEUTICAL INC COMMON STOCK USD.001     | CUSIP: 90400D108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     16546 | NS      | $601612.56    | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation                   | UNITED THERAPEUTICS CORP COMMON STOCK USD.01           | CUSIP: 91307C102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1551 | NS      | $445679.85    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                           | INTUITIVE SURGICAL INC COMMON STOCK USD.001            | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |     22909 | NS      | $12448979.69  | 5.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Mettler-Toledo International Inc.                 | METTLER-TOLEDO INTERNATIONAL I COMMON STOCK USD.01     | CUSIP: 592688105<br>LEI: 5493000BD5GJNUDIUG10 | Long             | EC               | CORP              | US        |       692 | NS      | $812906.24    | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Incorporated                   | UNITEDHEALTH GROUP INC COMMON STOCK USD.01             | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |     34934 | NS      | $10898359.98  | 5.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Universal Health Services Inc.                    | UNIVERSAL HEALTH SERVICES INC COMMON STOCK USD.01      | CUSIP: 913903100<br>LEI: 549300YGMGQDO0NMJD09 | Long             | EC               | CORP              | US        |      1300 | NS      | $235495.00    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Molina Healthcare Inc.                            | MOLINA HEALTHCARE INC COMMON STOCK USD.001             | CUSIP: 60855R100<br>LEI: 549300NQQCEQ46YHZ591 | Long             | EC               | CORP              | US        |      4954 | NS      | $1475796.60   | 0.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Roivant Sciences Ltd.                             | ROIVANT SCIENCES LTD COMMON STOCK USD.0000001          | CUSIP: G76279101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18400 | NS      | $207368.00    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc.                                      | VAXCYTE INC COMMON STOCK USD.001                       | CUSIP: 92243G108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     32110 | NS      | $1043896.10   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Veeva Systems Inc.                                | VEEVA SYSTEMS INC COMMON STOCK USD.00001               | CUSIP: 922475108<br>LEI: 549300YZEXPDB92M2F02 | Long             | EC               | CORP              | US        |      3109 | NS      | $895329.82    | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| MediWound Ltd                                     | MEDIWOUND LTD COMMON STOCK ILS.01                      | CUSIP: M68830112<br>LEI: N/A                  | Long             | EC               | CORP              | IL        |      5415 | NS      | $104888.55    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Vera Therapeutics Inc.                            | VERA THERAPEUTICS INC COMMON STOCK USD.001             | CUSIP: 92337R101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     27014 | NS      | $636449.84    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Verona Pharma PLC                                 | VERONA PHARMA PLC ADR                                  | CUSIP: 925050106<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |     26979 | NS      | $2551673.82   | 1.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated               | VERTEX PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |     18510 | NS      | $8240652.00   | 3.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Viridian Therapeutics Inc.                        | VIRIDIAN THERAPEUTICS INC COMMON STOCK USD.01          | CUSIP: 92790C104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10200 | NS      | $142596.00    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Voyager Therapeutics Inc.                         | VOYAGER THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 92915B106<br>LEI: 54930082S4XVR0ZWCC74 | Long             | EC               | CORP              | US        |     15964 | NS      | $49648.04     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Xencor Inc.                                       | XENCOR INC COMMON STOCK USD.01                         | CUSIP: 98401F105<br>LEI: 549300V5IF65437JKG30 | Long             | EC               | CORP              | US        |     13179 | NS      | $103586.94    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Zai Lab Limited                                   | ZAI LAB LTD ADR USD.00006                              | CUSIP: 98887Q104<br>LEI: N/A                  | Long             | EC               | CORP              | CN        |      4866 | NS      | $170164.02    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| BeOne Medicines Ltd                               | BEONE MEDICINES LTD COMMON STOCK USD.0001              | CUSIP: BVDKGC900<br>LEI: 549300JFUK6FRD5MH739 | Long             | EC               | CORP              | US        |    157998 | NS      | $2988853.38   | 1.41%             |  |  |  | No            |                  2 | On Loan: No      |
| Sensorion                                         | SENSORION SA COMMON STOCK EUR.1                        | CUSIP: BWSW4N905<br>LEI: N/A                  | Long             | EC               | CORP              | FR        |    182870 | NS      | $67343.99     | 0.03%             |  |  |  | No            |                  2 | On Loan: No      |
| Alkermes Public Limited Company                   | ALKERMES PLC COMMON STOCK USD.01                       | CUSIP: G01767105<br>LEI: 549300YHRMYLL071E243 | Long             | EC               | CORP              | IE        |     28133 | NS      | $804885.13    | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Biohaven Ltd.                                     | BIOHAVEN LTD COMMON STOCK                              | CUSIP: G1110E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     39446 | NS      | $556583.06    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Merus N.V.                                        | MERUS NV COMMON STOCK EUR.05                           | CUSIP: N5749R100<br>LEI: 549300W8GPUWU8QNU293 | Long             | EC               | CORP              | NL        |     10580 | NS      | $556508.00    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Pharvaris N.V.                                    | PHARVARIS NV COMMON STOCK EUR.12                       | CUSIP: N69605108<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |     11645 | NS      | $204952.00    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Wave Life Sciences Ltd.                           | WAVE LIFE SCIENCES LTD COMMON STOCK                    | CUSIP: Y95308105<br>LEI: 54930070006GPKFYZ813 | Long             | EC               | CORP              | US        |     28868 | NS      | $187642.00    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Sonova Holding AG                                 | SONOVA HOLDING AG COMMON STOCK CHF.05                  | CUSIP: 715603908<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |      3871 | NS      | $1154430.52   | 0.54%             |  |  |  | No            |                  2 | On Loan: No      |
| Praxis Precision Medicines Inc.                   | PRAXIS PRECISION MEDICINES INC COMMON STOCK USD.0001   | CUSIP: 74006W207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8966 | NS      | $377020.30    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Prelude Therapeutics Incorporated                 | PRELUDE THERAPEUTICS INC COMMON STOCK USD.0001         | CUSIP: 74065P101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17428 | NS      | $14106.22     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Procept Biorobotics Corporation                   | PROCEPT BIOROBOTICS CORP COMMON STOCK USD.00001        | CUSIP: 74276L105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14588 | NS      | $840268.80    | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| SomaLogic Inc.                                    | SOMALOGIC INC WARRANTS CALL EXP 31AUG26                | CUSIP: 83444K113<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2050 | NS      | $231.55       | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| Stryker Corporation                               | STRYKER CORP COMMON STOCK USD.1                        | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |     27006 | NS      | $10684383.78  | 5.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Structure Therapeutics Inc.                       | STRUCTURE THERAPEUTICS INC ADR USD.0001                | CUSIP: 86366E106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7577 | NS      | $157146.98    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Summit Therapeutics Inc.                          | SUMMIT THERAPEUTICS INC COMMON STOCK USD.01            | CUSIP: 86627T108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     57081 | NS      | $1214683.68   | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                       | ABBVIE INC COMMON STOCK USD.01                         | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |     20752 | NS      | $3851986.24   | 1.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Acadia Pharmaceuticals Inc.                       | ACADIA PHARMACEUTICALS INC COMMON STOCK USD.0001       | CUSIP: 004225108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5100 | NS      | $110007.00    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Spyre Therapeutics Inc.                           | SPYRE THERAPEUTICS INC COMMON STOCK USD.0001           | CUSIP: 00773J202<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13120 | NS      | $196406.40    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Agios Pharmaceuticals Inc.                        | AGIOS PHARMACEUTICALS INC COMMON STOCK USD.001         | CUSIP: 00847X104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21152 | NS      | $703515.52    | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Akero Therapeutics Inc.                           | AKERO THERAPEUTICS INC COMMON STOCK USD.0001           | CUSIP: 00973Y108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22529 | NS      | $1202147.44   | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Alector Inc.                                      | ALECTOR INC COMMON STOCK USD.0001                      | CUSIP: 014442107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     73204 | NS      | $102485.60    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Alignment Healthcare Inc.                         | ALIGNMENT HEALTHCARE INC COMMON STOCK USD.001          | CUSIP: 01625V104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     35631 | NS      | $498834.00    | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Allogene Therapeutics Inc.                        | ALLOGENE THERAPEUTICS INC COMMON STOCK USD.001         | CUSIP: 019770106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     81376 | NS      | $91954.88     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Alnylam Pharmaceuticals Inc.                      | ALNYLAM PHARMACEUTICALS INC COMMON STOCK USD.01        | CUSIP: 02043Q107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     19364 | NS      | $6314406.76   | 2.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Cencora Inc.                                      | CENCORA INC COMMON STOCK USD.01                        | CUSIP: 03073E105<br>LEI: AI8GXW8LG5WK7E9UD086 | Long             | EC               | CORP              | US        |      5603 | NS      | $1680059.55   | 0.79%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                                        | AMGEN INC COMMON STOCK USD.0001                        | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |      1573 | NS      | $439197.33    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Annexon Inc.                                      | ANNEXON INC COMMON STOCK                               | CUSIP: 03589W102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22109 | NS      | $53061.60     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Elevance Health Inc.                              | ELEVANCE HEALTH INC COMMON STOCK USD.01                | CUSIP: 036752103<br>LEI: 8MYN82XMYQH89CTMTH67 | Long             | EC               | CORP              | US        |     10881 | NS      | $4232273.76   | 1.99%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                               | ASCENDIS PHARMA A/S ADR DKK1.0                         | CUSIP: 04351P101<br>LEI: N/A                  | Long             | EC               | CORP              | DK        |     13535 | NS      | $2336141.00   | 1.10%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                                   | ASTRAZENECA PLC ADR                                    | CUSIP: 046353108<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |     37856 | NS      | $2645377.28   | 1.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Aura Biosciences Inc.                             | AURA BIOSCIENCES INC COMMON STOCK USD.00001            | CUSIP: 05153U107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10426 | NS      | $65266.76     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.                          | AVIDITY BIOSCIENCES INC COMMON STOCK                   | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     47552 | NS      | $1350476.80   | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Axsome Therapeutics Inc.                          | AXSOME THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 05464T104<br>LEI: 549300EXGR0XLRJEIB95 | Long             | EC               | CORP              | US        |      1500 | NS      | $156585.00    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Bicara Therapeutics Inc.                          | BICARA THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 055477103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     31071 | NS      | $288649.59    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Beam Therapeutics Inc.                            | BEAM THERAPEUTICS INC COMMON STOCK USD.01              | CUSIP: 07373V105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     24279 | NS      | $412985.79    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| BeOne Medicines Ltd.                              | BEONE MEDICINES LTD ADR USD.0001                       | CUSIP: 07725L102<br>LEI: 549300JFUK6FRD5MH739 | Long             | EC               | CORP              | US        |       360 | NS      | $87145.20     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Beta Bionics Inc.                                 | BETA BIONICS INC COMMON STOCK USD.0001                 | CUSIP: 08659B102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4325 | NS      | $62972.00     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Bicycle Therapeutics plc                          | BICYCLE THERAPEUTICS PLC ADR USD.01                    | CUSIP: 088786108<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |     25602 | NS      | $177933.90    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Blueprint Medicines Corporation                   | BLUEPRINT MEDICINES CORP COMMON STOCK USD.001          | CUSIP: 09627Y109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9602 | NS      | $1230784.36   | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation                     | BOSTON SCIENTIFIC CORP COMMON STOCK USD.01             | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |     73791 | NS      | $7925891.31   | 3.73%             |  |  |  | No            |                  1 | On Loan: No      |
| BrightSpring Health Services Inc.                 | BRIGHTSPRING HEALTH SERVICES I COMMON STOCK USD100.0   | CUSIP: 10950A106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     39334 | NS      | $927889.06    | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cigna Group                                   | CIGNA GROUP/THE COMMON STOCK USD.01                    | CUSIP: 125523100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13901 | NS      | $4595392.58   | 2.16%             |  |  |  | No            |                  1 | On Loan: No      |
| CVS Health Corporation                            | CVS HEALTH CORP COMMON STOCK USD.01                    | CUSIP: 126650100<br>LEI: 549300EJG376EN5NQE29 | Long             | EC               | CORP              | US        |     19771 | NS      | $1363803.58   | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Cabaletta Bio Inc.                                | CABALETTA BIO INC COMMON STOCK USD.00001               | CUSIP: 12674W109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9787 | NS      | $14876.24     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Cardinal Health Inc.                              | CARDINAL HEALTH INC COMMON STOCK                       | CUSIP: 14149Y108<br>LEI: CCU46N3GJMF4OK4N7U60 | Long             | EC               | CORP              | US        |      6020 | NS      | $1011360.00   | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| CARGO Therapeutics Inc.                           | CARGO THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 14179K101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      6153 | NS      | $25350.36     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Caris Life Sciences Inc.                          | CARIS LIFE SCIENCES INC COMMON STOCK USD.001           | CUSIP: 142152107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5439 | NS      | $145330.08    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Crinetics Pharmaceuticals Inc.                    | CRINETICS PHARMACEUTICALS INC COMMON STOCK USD.001     | CUSIP: 22663K107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     22567 | NS      | $649026.92    | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Cytokinetics Incorporated                         | CYTOKINETICS INC COMMON STOCK USD.001                  | CUSIP: 23282W605<br>LEI: 549300BE5DY1HW7IHA64 | Long             | EC               | CORP              | US        |      9735 | NS      | $321644.40    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                               | DANAHER CORP COMMON STOCK USD.01                       | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |     33834 | NS      | $6683568.36   | 3.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Denali Therapeutics Inc.                          | DENALI THERAPEUTICS INC COMMON STOCK USD.01            | CUSIP: 24823R105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     53705 | NS      | $751332.95    | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| DexCom Inc.                                       | DEXCOM INC COMMON STOCK USD.001                        | CUSIP: 252131107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7020 | NS      | $612775.80    | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Disc Medicine Inc.                                | DISC MEDICINE INC COMMON STOCK USD.0001                | CUSIP: 254604101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7393 | NS      | $391533.28    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Climb Bio Inc.                                    | CLIMB BIO INC COMMON STOCK USD.0001                    | CUSIP: 28658R106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9200 | NS      | $11408.00     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Encompass Health Corporation                      | ENCOMPASS HEALTH CORP COMMON STOCK USD.01              | CUSIP: 29261A100<br>LEI: 549300FVRPM9DRQZRG64 | Long             | EC               | CORP              | US        |      4586 | NS      | $562381.18    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Enliven Therapeutics Inc.                         | ENLIVEN THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 29337E102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7709 | NS      | $154642.54    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Entrada Therapeutics Inc                          | ENTRADA THERAPEUTICS INC COMMON STOCK USD.0001         | CUSIP: 29384C108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11625 | NS      | $78120.00     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Exact Sciences Corporation                        | EXACT SCIENCES CORP COMMON STOCK USD.01                | CUSIP: 30063P105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20421 | NS      | $1085171.94   | 0.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Generation Bio Co.                                | GENERATION BIO CO COMMON STOCK USD.0001                | CUSIP: 37148K100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     37692 | NS      | $12065.21     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Gilead Sciences Inc.                              | GILEAD SCIENCES INC COMMON STOCK USD.001               | CUSIP: 375558103<br>LEI: 549300WTZWR07K8MNV44 | Long             | EC               | CORP              | US        |     46984 | NS      | $5209116.08   | 2.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Ginkgo Bioworks Holdings Inc.                     | GINKGO BIOWORKS HOLDINGS INC COMMON STOCK USD.0001     | CUSIP: 37611X209<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1971 | NS      | $22173.75     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Guardant Health Inc.                              | GUARDANT HEALTH INC COMMON STOCK USD.00001             | CUSIP: 40131M109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20059 | NS      | $1043870.36   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| HCA Healthcare Inc.                               | HCA HEALTHCARE INC COMMON STOCK USD.01                 | CUSIP: 40412C101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4490 | NS      | $1720119.00   | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Heron Therapeutics Inc.                           | HERON THERAPEUTICS INC COMMON STOCK USD.01             | CUSIP: 427746102<br>LEI: 549300DR2I1DOPD2IX53 | Long             | EC               | CORP              | US        |     43200 | NS      | $89424.00     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEAYA Biosciences Inc.                           | IDEAYA BIOSCIENCES INC COMMON STOCK                    | CUSIP: 45166A102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8737 | NS      | $183651.74    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEXX Laboratories Inc.                           | IDEXX LABORATORIES INC COMMON STOCK USD.1              | CUSIP: 45168D104<br>LEI: OGMTXK0LUU1HKV2P0J84 | Long             | EC               | CORP              | US        |      1698 | NS      | $910705.32    | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunocore Holdings plc                           | IMMUNOCORE HOLDINGS PLC SR UNSECURED 02/30 2.5         | CUSIP: 45258DAB1<br>LEI: N/A                  | Long             | DBT              | CORP              | GB        |    277000 | PA      | $238864.86    | 0.11%             | 2030-02-01      | Fixed         | 2.50%                 | No            |                  2 | On Loan: No      |
| Ionis Pharmaceuticals Inc.                        | IONIS PHARMACEUTICALS INC COMMON STOCK USD.001         | CUSIP: 462222100<br>LEI: 549300SI4ZGLG0BLUZ92 | Long             | EC               | CORP              | US        |     13309 | NS      | $525838.59    | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Iovance Biotherapeutics Inc.                      | IOVANCE BIOTHERAPEUTICS INC COMMON STOCK USD.000041666 | CUSIP: 462260100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     31687 | NS      | $54501.64     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Janux Therapeutics Inc.                           | JANUX THERAPEUTICS INC COMMON STOCK USD.001            | CUSIP: 47103J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10117 | NS      | $233702.70    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Krystal Biotech Inc.                              | KRYSTAL BIOTECH INC COMMON STOCK USD.00001             | CUSIP: 501147102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3168 | NS      | $435473.28    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Kymera Therapeutics Inc.                          | KYMERA THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 501575104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     28002 | NS      | $1222007.28   | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Kyverna Therapeutics Inc.                         | KYVERNA THERAPEUTICS INC COMMON STOCK USD.00001        | CUSIP: 501976104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     27613 | NS      | $84771.91     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc.                            | LANTHEUS HOLDINGS INC COMMON STOCK USD.01              | CUSIP: 516544103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3453 | NS      | $282662.58    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Legend Biotech Corporation                        | LEGEND BIOTECH CORP ADR USD.0001                       | CUSIP: 52490G102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13773 | NS      | $488803.77    | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly and Company                             | ELI LILLY and CO COMMON STOCK                          | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |     32643 | NS      | $25446197.79  | 11.96%            |  |  |  | No            |                  1 | On Loan: No      |
| Lyell Immunopharma Inc.                           | LYELL IMMUNOPHARMA INC COMMON STOCK USD.0001           | CUSIP: 55083R203<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      6196 | NS      | $54772.64     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| MBX Biosciences Inc.                              | MBX BIOSCIENCES INC COMMON STOCK USD.0001              | CUSIP: 55287L101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26494 | NS      | $302296.54    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Madrigal Pharmaceuticals Inc.                     | MADRIGAL PHARMACEUTICALS INC COMMON STOCK USD.0001     | CUSIP: 558868105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3063 | NS      | $926986.32    | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| U C B SA                                          | UCB SA COMMON STOCK                                    | CUSIP: 559699905<br>LEI: N/A                  | Long             | EC               | CORP              | BE        |      1373 | NS      | $270148.43    | 0.13%             |  |  |  | No            |                  2 | On Loan: No      |
| Masimo Corporation                                | MASIMO CORP COMMON STOCK USD.001                       | CUSIP: 574795100<br>LEI: 549300Q4A8JK0PIYCU29 | Long             | EC               | CORP              | US        |      5512 | NS      | $927228.64    | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| McKesson Corporation                              | MCKESSON CORP COMMON STOCK USD.01                      | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |       433 | NS      | $317293.74    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Sartorius Aktiengesellschaft                      | SARTORIUS AG PREFERENCE                                | CUSIP: 584332902<br>LEI: N/A                  | Long             | EP               | CORP              | DE        |      2158 | NS      | $549638.04    | 0.26%             |  |  |  | No            |                  2 | On Loan: No      |
| Metsera Inc.                                      | METSERA INC COMMON STOCK USD.00001                     | CUSIP: 59267L107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10966 | NS      | $311982.70    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Monte Rosa Therapeutics Inc.                      | MONTE ROSA THERAPEUTICS INC COMMON STOCK USD.0001      | CUSIP: 61225M102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     29932 | NS      | $134993.32    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| MoonLake Immunotherapeutics                       | MOONLAKE IMMUNOTHERAPEUTICS COMMON STOCK USD.0001      | CUSIP: 61559X104<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |     22471 | NS      | $1060631.20   | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Tenet Healthcare Corporation                      | TENET HEALTHCARE CORP COMMON STOCK USD.05              | CUSIP: 88033G407<br>LEI: 0W9AIBT6U6ADJ2I8HK17 | Long             | EC               | CORP              | US        |     12160 | NS      | $2140160.00   | 1.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Helix Acquisition Corp. II                        | HELIX ACQUISITION CORP II COMMON STOCK USD.0001        | CUSIP: G4444H101<br>LEI: N/A                  | Long             | EC               | CORP              | KY        |     16298 | NS      | $173573.70    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Icon Public Limited Company                       | ICON PLC COMMON STOCK EUR.06                           | CUSIP: G4705A100<br>LEI: N/A                  | Long             | EC               | CORP              | IE        |      1899 | NS      | $276209.55    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Kestra Medical Technologies Ltd                   | KESTRA MEDICAL TECHNOLOGIES LT COMMON STOCK USD100.0   | CUSIP: G52441105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9069 | NS      | $150364.02    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| UroGen Pharma Ltd.                                | UROGEN PHARMA LTD COMMON STOCK USD.01                  | CUSIP: M96088105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5500 | NS      | $75350.00     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2025-07-30

**Name of Applicant:** John Hancock Variable Insurance Trust

**Signature:** /s/Salvatore Schiavone

**Name of Signer:** Salvatore Schiavone

**Title:** Treasurer