# EDGAR Filing Document

**Accession Number:** 0000926617
**File Stem:** 0000926617-26-000020
**Filing Date:** 2026-4
**Character Count:** 420505
**Document Hash:** 3c437ee51b533cffaed41dc1c0cd172d
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0000926617-26-000020.hdr.sgml**: 20260401

**ACCESSION NUMBER**: 0000926617-26-000020

**CONFORMED SUBMISSION TYPE**: 10-K

**PUBLIC DOCUMENT COUNT**: 82

**CONFORMED PERIOD OF REPORT**: 20251231

**FILED AS OF DATE**: 20260401

**DATE AS OF CHANGE**: 20260401

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Aspira Women's Health Inc.
- **CENTRAL INDEX KEY:** 0000926617
- **STANDARD INDUSTRIAL CLASSIFICATION:** IN VITRO & IN VIVO DIAGNOSTIC SUBSTANCES [2835]
- **ORGANIZATION NAME:** 03 Life Sciences
- **EIN:** 330595156
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** 10-K
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 001-34810
- **FILM NUMBER:** 26827903

**BUSINESS ADDRESS:**
- **STREET 1:** 12117 BEE CAVES ROAD BUILDING THREE
- **STREET 2:** SUITE 100
- **CITY:** AUSTIN
- **STATE:** TX
- **ZIP:** 78738
- **BUSINESS PHONE:** 512-519-0400

**MAIL ADDRESS:**
- **STREET 1:** 12117 BEE CAVES ROAD BUILDING THREE
- **STREET 2:** SUITE 100
- **CITY:** AUSTIN
- **STATE:** TX
- **ZIP:** 78738

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** VERMILLION, INC.
- **DATE OF NAME CHANGE:** 20070824

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** CIPHERGEN BIOSYSTEMS INC
- **DATE OF NAME CHANGE:** 20000316

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** ABIOTIC SYSTEMS
- **DATE OF NAME CHANGE:** 19950407

?xml version='1.0' encoding='ASCII'? Aspira Women's Health Inc._December 31, 2025

[**Table of Contents**](#TOC)

**licensing**

**UNITED STATES**

**SECURITIES AND EXCHANGE COMMISSION**

**Washington, D. C. 20549**

**FORM 10-K**

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| | |
|:---|:---|
| **☒** | **ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934** |
|  | For the fiscal year ended December 31, 2025 or |
| ☐ | **TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934** |
|  | For the transition period from &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; to &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;  |

---

Commission file number: 001-34810

**Aspira Women's Health Inc.**

(Exact name of registrant as specified in its charter)

---

| | |
|:---|:---|
| **Delaware** | **33-0595156** |
| (State or Other Jurisdiction of Incorporation or Organization) | (I.R.S. Employer Identification No.) |
| **12117 Bee Caves Road, Building III, Suite 100** |  |
| **Austin, Texas** | **78738** |
| (Address of Principal Executive Offices) | (Zip Code) |

---

**Registrant's telephone number, including area code: (512) 519-0400**

**Securities registered pursuant to Section 12(b) of the Act:**

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| | | |
|:---|:---|:---|
| Title of each class  | Trading Symbol(s) | Name of each exchange on which registered  |
| **Common Stock, par value $0.001 per share** | **AWHL** | **The OTC QX Market** |

---

**Securities registered pursuant to Section 12(g) of the Act: None**

Indicate by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act. Yes ☐ No **☒**

Indicate by check mark if the registrant is not required to file reports pursuant to Section 13 or Section 15(d) of the Act. Yes ☐ No **☒**

Indicate by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2) has been subject to such filing requirements for the past 90 days. Yes **☒** No ☐

Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S-T (§ 232.405 of this chapter) during the preceding 12 months (or for such shorter period that the registrant was required to submit such files). Yes **☒** No ☐

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company, or an emerging growth company. See the definitions of "large accelerated filer," "accelerated filer," "smaller reporting company" and "emerging growth company" in Rule 12b-2 of the Exchange Act.

---

| | |
|:---|:---|
| Large accelerated filer**☐** | Accelerated filer **☐** |
| Non-accelerated filer **☒** | Smaller reporting company **☒** |
|  | Emerging growth company **☐** |

---

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. **☐**

Indicate by check mark whether the registrant has filed a report on and attestation to its management's assessment of the effectiveness of its internal control over financial reporting under Section 404(b) of the Sarbanes-Oxley Act (15 U.S.C. 7262(b)) by the registered public accounting firm that prepared or issued its audit report. Yes ☐ No **☒**

If securities are registered pursuant to Section 12(b) of the Act, indicate by check mark whether the financial statements of the registrant included in the filing reflect the correction of an error to previously issued financial statements. **☐**

Indicate by check mark whether any of those error corrections are restatements that required a recovery analysis of incentive-based compensation received by any of the registrant's executive officers during the relevant recovery period pursuant to §240.10D-1(b). ☐

Indicate by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Act). Yes ☐ No **☒**

The aggregate market value of voting common stock held by non-affiliates of the registrant is $4,669,804 and is based upon the last sales price as quoted on The OTC QX Best Market as of June 30, 2025.

As of March 30, 2026, the registrant had 43,500,411 shares of common stock, par value $0.001 per share, outstanding.

**DOCUMENTS INCORPORATED BY REFERENCE**

Certain information from the registrant's Definitive Proxy Statement for its 2026 Annual Meeting of Stockholders is incorporated by reference into Part III of this report.

------

[**Table of Contents**](#TOC)

#### 4ASPIRA WOMEN'S HEALTH INC.
**Table of Contents**

---

| | | | |
|:---|:---|:---|:---|
|  |  |  | **PageNo.** |
| [PART I](#PARTI_248729) | [PART I](#PARTI_248729) | [PART I](#PARTI_248729) | 7 |
|  | [ITEM 1.](#ITEM1BUSINESS_234840) | [Business](#ITEM1BUSINESS_234840) | 7 |
|  | [ITEM 1A.](#ITEM1ARISKFACTORS_433980) | [Risk Factors](#ITEM1ARISKFACTORS_433980) | 25 |
|  | [ITEM 1B.](#ITEM1BUNRESOLVEDSTAFFCOMMENTS_607250) | [Unresolved Staff Comments](#ITEM1BUNRESOLVEDSTAFFCOMMENTS_607250) | 43 |
|  | [ITEM 1C.](#ITEM1CCYBERSECURITY_928916) | [Cybersecurity](#ITEM1CCYBERSECURITY_928916) | 43 |
|  | [ITEM 2.](#ITEM2PROPERTIES_726598) | [Properties](#ITEM2PROPERTIES_726598) | 44 |
|  | [ITEM 3.](#ITEM3LEGALPROCEEDINGS_215906) | [Legal Proceedings](#ITEM3LEGALPROCEEDINGS_215906) | 44 |
|  | [ITEM 4.](#ITEM4MINESAFETYDISCLOSURES_663525) | [Mine Safety Disclosures](#ITEM4MINESAFETYDISCLOSURES_663525) | 44 |
| [PART II](#PARTII_43112) | [PART II](#PARTII_43112) | [PART II](#PARTII_43112) | 45 |
|  | [ITEM 5.](#ITEM5MARKETFORREGISTRANTSCOMMONEQUITYREL) | [Market For Registrant's Common Equity, Related Stockholder Matters and Issuer Purchases of Equity Securities](#ITEM5MARKETFORREGISTRANTSCOMMONEQUITYREL) | 45 |
|  | [ITEM 6.](#ITEM6Reserved_76633) | [\[Reserved\]](#ITEM6Reserved_76633) | 45 |
|  | [ITEM 7.](#ITEM7MANAGEMENTSDISCUSSIONANDANALYSISOFF) | [Management's Discussion and Analysis of Financial Condition and Results of Operations](#ITEM7MANAGEMENTSDISCUSSIONANDANALYSISOFF) | 46 |
|  | [ITEM 7A.](#ITEM7AQUANTITATIVEANDQUALITATIVEDISCLOSU) | [Quantitative and Qualitative Disclosures About Market Risk](#ITEM7AQUANTITATIVEANDQUALITATIVEDISCLOSU) | 59 |
|  | [ITEM 8.](#ITEM8CONSOLIDATEDFINANCIALSTATEMENTSANDS) | [Consolidated Financial Statements and Supplementary Data](#ITEM8CONSOLIDATEDFINANCIALSTATEMENTSANDS) | 59 |
|  | [ITEM 9.](#ITEM9CHANGESINANDDISAGREEMENTSWITHACCOUN) | [Changes in and Disagreements With Accountants on Accounting and Financial Disclosure](#ITEM9CHANGESINANDDISAGREEMENTSWITHACCOUN) | 59 |
|  | [ITEM 9A.](#ITEM9ACONTROLSANDPROCEDURES_492867) | [Controls and Procedures](#ITEM9ACONTROLSANDPROCEDURES_492867) | 59 |
|  | [ITEM 9B.](#ITEM9BOTHERINFORMATION_872193) | [Other Information](#ITEM9BOTHERINFORMATION_872193) | 61 |
|  | [ITEM 9C.](#ITEM9CDISCLOSUREREGARDINGFOREIGNJURISDIC) | [Disclosure Regarding Foreign Jurisdictions that Prevent Inspections](#ITEM9CDISCLOSUREREGARDINGFOREIGNJURISDIC) | 61 |
| [PART III](#PARTIII_362810) | [PART III](#PARTIII_362810) | [PART III](#PARTIII_362810) | 61 |
|  | [ITEM 10.](#ITEM10DIRECTORSEXECUTIVEOFFICERSANDCORPO) | [Directors, Executive Officers and Corporate Governance](#ITEM10DIRECTORSEXECUTIVEOFFICERSANDCORPO) | 61 |
|  | [ITEM 11.](#ITEM11EXECUTIVECOMPENSATION_265057) | [Executive Compensation](#ITEM11EXECUTIVECOMPENSATION_265057) | 61 |
|  | [ITEM 12.](#ITEM12SECURITYOWNERSHIPOFCERTAINBENEFICI) | [Security Ownership of Certain Beneficial Owners and Management and Related Stockholder Matters](#ITEM12SECURITYOWNERSHIPOFCERTAINBENEFICI) | 61 |
|  | [ITEM 13.](#ITEM13CERTAINRELATIONSHIPSANDRELATEDTRAN) | [Certain Relationships and Related Transactions, and Director Independence](#ITEM13CERTAINRELATIONSHIPSANDRELATEDTRAN) | 62 |
|  | [ITEM 14.](#ITEM14PRINCIPALACCOUNTANTFEESANDSERVICES) | [Principal Accountant Fees and Services](#ITEM14PRINCIPALACCOUNTANTFEESANDSERVICES) | 62 |
| [PART IV](#PARTIV_186839) | [PART IV](#PARTIV_186839) | [PART IV](#PARTIV_186839) | 62 |
|  | [ITEM 15.](#ITEM15EXHIBITSFINANCIALSTATEMENTSCHEDULE) | [Exhibits and Financial Statement Schedules](#ITEM15EXHIBITSFINANCIALSTATEMENTSCHEDULE) | 62 |
|  | [ITEM 16.](#ITEM16FORM10KSUMMARY_475128) | [Form 10-K Summary](#ITEM16FORM10KSUMMARY_475128) | 65 |
| [SIGNATURES](#SIGNATURES_973677) | [SIGNATURES](#SIGNATURES_973677) | [SIGNATURES](#SIGNATURES_973677) | 68 |

---

*The following are registered and unregistered trademarks and service marks of Aspira Women's Health Inc.: VERMILLION*<sup>SM</sup>*, ASPIRA WOMEN'S HEALTH*<sup>®</sup>*, OVA1*<sup>®</sup>*, OVERA*<sup>®</sup>*, ASPIRA LABS*<sup>®</sup>*, OVACALC*<sup>®</sup>*, OVASUITE*<sup>SM</sup>*, ASPIRA GENETIX*<sup>SM</sup> *, OVA1PLUS*<sup>®</sup>*, OVAWATCH*<sup>®</sup>*, ENDOCHECK*<sup>SM</sup>*, ENDOINFORM*<sup>TM</sup>*, OVAINFORM*<sup>TM</sup>*, OVAINHERIT*<sup>SM</sup>*, ASPIRA SYNERGY*<sup>®,</sup>*, OVA360*<sup>SM</sup>*, ASPIRA IVD*<sup>SM</sup>*, and YOUR HEALTH, OUR PASSION*<sup>®</sup>*.*

i

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#### SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS
This Annual Report on Form 10-K contains forward-looking statements, within the meaning of Section 27A of the Securities Act of 1933, as amended (the "Securities Act"), and Section 21E of the Securities Exchange Act of 1934, as amended (the "Exchange Act"), about us and our industry that involve substantial risks and uncertainties.

These statements involve a number of risks and uncertainties. Words such as "may," "expects," "intends," "anticipates," "believes," "estimates," "plans," "seeks," "could," "should," "continue," "will," "potential," "targeted," "predicts," "projects," "aim" and similar expressions are intended to identify such forward-looking statements. Readers are cautioned that these forward-looking statements speak only as of the date on which this report was filed with the Securities and Exchange Commission (the "SEC"), and, except as required by law, Aspira Women's Health Inc. ("Aspira" and, together with its subsidiaries, the "Company," "we," "our," or "us") does not assume any obligation to update, amend or clarify them to reflect events, new information or circumstances occurring after such date.

Examples of forward-looking statements include, without limitation:

● projections or expectations regarding our future test volumes, revenue, average unit price, cost of revenue, operating expenses, research and development expenses, gross profit margin, cash flow, results of operations and financial condition;

● our plan to broaden our commercial focus from ovarian cancer to differential diagnosis of women with a range of gynecological diseases, including additional pelvic disease conditions such as endometriosis and benign pelvic mass monitoring;

● our planned business strategy and the anticipated effects thereof, including partnerships such as those based on our Aspira Synergy platform, specimen or research collaborations, licensing arrangements, commercial collaborations and distribution agreements;

● plans to expand our current or future products to markets outside of the United States through distribution collaborations or out-licensing;

● plans to develop new algorithms, molecular diagnostic tests, products and tools and otherwise expand our product offerings;

● plans to develop, launch and establish payer coverage and secure contracts for current and new products, including ENDOinform and OVAinform;

● expectations regarding local and/or national coverage under Novitas, our Medicare Administrative Carrier;

● anticipated efficacy of our products, product development activities and product innovations, including our ability to improve sensitivity and specificity over traditional diagnostics;

● expected competition in the markets in which we operate;

● plans with respect to Aspira Labs, Inc. ("Aspira Labs"), including plans to expand Aspira Labs' testing capabilities, specifically molecular lab capabilities;

● expectations regarding continuing future services provided by Quest Diagnostics Incorporated;

● expectations regarding continuing future services provided by BioReference Health, LLC;

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● plans to develop informatics products as laboratory developed tests ("LDTs") and potential Food and Drug Administration ("FDA") oversight changes of LDTs;

● expectations regarding existing and future collaborations and partnerships for our products, including plans to enter into decentralized arrangements for our Aspira Synergy platform and to provide and expand access to our risk assessment tests;

● plans regarding future publications and presentations;

● expectations regarding potential collaborations with governments, legislative bodies and advocacy groups to enhance awareness and drive policies to provide broader access to our tests;

● our ability to continue to comply with applicable governmental regulations, including regulations applicable to the operation of our clinical lab, expectations regarding pending regulatory submissions and plans to seek regulatory approvals for our tests within the United States and internationally, as applicable;

● our continued ability to expand and protect our intellectual property portfolio;

● anticipated liquidity and capital requirements;

● anticipated future losses and our ability to continue as a going concern;

● expectations regarding raising capital and the amount of financing anticipated to be required to fund our planned operations;

● expectations regarding attrition and recruitment of top talent;

● expectations regarding the results of our clinical research studies and our ability to recruit patients to participate in such studies;

● our ability to use our net operating loss carryforwards and anticipated future tax liability under U.S. federal and state income tax legislation;

● expected market adoption of our current and prospective diagnostic tests, including Ova1, Overa, Ova1Plus, OvaWatch, ENDOinform and OVAinform, as well as our Aspira Synergy platform;

● expectations regarding our ability to launch new products we develop, license, co-market or acquire;

● expectations regarding the size of the markets for our products;

● expectations regarding reimbursement for our products, and our ability to obtain such reimbursement, from third-party payers such as private insurance companies and government insurance plans;

● potential plans to pursue clearance designation with the FDA with respect to OvaWatch, ENDOinform and OVAinform;

● expected potential target launch timing for future products;

● expectations regarding compliance with federal and state laws and regulations relating to billing arrangements conducted in coordination with laboratories;

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● plans to advocate for legislation and professional society guidelines to broaden access to our products and services;

● ability to protect and safeguard against cybersecurity risks and breaches; and

● expectations regarding the results of our academic research agreements.

We caution you that the foregoing list may not contain all of the forward-looking statements made in this Annual Report on Form 10-K.

Other sections of this Annual Report on Form 10-K may include additional factors that could harm our business and financial performance. Moreover, we operate in a very competitive and rapidly changing environment. New risk factors emerge from time to time, and it is not possible for our management to predict all risk factors, nor can we assess the impact of all factors on our business or the extent to which any factor, or combination of factors, may cause actual results to differ materially from those contained in, or implied by, any forward-looking statements.

Forward-looking statements are subject to significant risks and uncertainties, including those discussed in Part I Item 1A, "Risk Factors," that could cause actual results to differ materially from those projected in such forward-looking statements due to various factors, including our ability to continue as a going concern; impacts resulting from potential changes to coverage of Ova1 through our Medicare Administrative Carrier for Ova1; anticipated use of capital and its effects; our ability to increase the volume of our product sales; failures by third-party payers to reimburse for our products and services or changes to reimbursement rates; our ability to continue developing existing technologies and to develop, protect and promote our proprietary technologies; plans to develop and perform LDTs; our ability to comply with FDA regulations that relate to our products and to obtain any FDA clearance or approval required to develop and commercialize medical devices; our ability to develop and commercialize additional diagnostic products and achieve market acceptance with respect to these products; our ability to compete successfully; our ability to obtain any regulatory approval required for our future diagnostic products; our or our suppliers' ability to comply with FDA requirements for production, marketing and post-market monitoring of our products; our ability to maintain sufficient or acceptable supplies of immunoassay kits from our suppliers; in the event that we succeed in commercializing our products outside the United States, the political, economic and other conditions affecting other countries; changes in healthcare policy; our ability to comply with environmental laws; our ability to comply with the additional laws and regulations that apply to us in connection with the operation of Aspira Labs; our ability to use our net operating loss carryforwards; our ability to use intellectual property; our ability to successfully defend our proprietary technology against third parties; our ability to obtain licenses in the event a third party successfully asserts proprietary rights; the liquidity and trading volume of our common stock; the concentration of ownership of our common stock; our ability to retain key employees; our ability to secure additional capital on acceptable terms to execute our business plan; business interruptions or force majeure or acts of God; the effectiveness and availability of our information systems; our ability to integrate and achieve anticipated results from any acquisitions or strategic alliances; future litigation against us, including infringement of intellectual property and product liability exposure; and additional costs that may be required to make further improvements to our laboratory operations.

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#### SUMMARY OF RISK FACTORS
The following is only a summary of the principal risks that may materially adversely affect our business, financial condition, results of operations and cash flows. The following should be read in conjunction with the more complete discussion of the risk factors we face, which are set forth in the section entitled "Item 1A. Risk Factors" in this report.

#### Risks Related to Our Business and Industry
● If we are unable to increase the volume of OvaSuite sales, our business, results of operations and financial condition will be adversely affected.

● There is substantial doubt about our ability to continue as a going concern, and this may adversely affect our stock price and our ability to raise capital.

● We will need to sell additional shares of our common stock or other securities in the future to meet our capital requirements, which could cause significant dilution.

● We will need to raise additional capital in the future, and if we are unable to secure adequate funds on terms acceptable to us, we may be unable to execute our business plan.

● Failures by third-party payers to reimburse for our products and services or changes in reimbursement rates could materially and adversely affect our business, financial condition and results of operations. In addition, changes in medical society guidelines may also adversely affect payers and result in a material change in coverage, adversely affecting our business, financial condition and results of operations.

● Failure to continue coverage of Ova1 through Novitas, our Medicare Administrative Contractor for Ova1, could materially and adversely affect our business, financial condition and results of operation.

● Failure to expand commercial, Medicare or Medicaid coverage for our products could materially and adversely affect our business, financial condition and results of operations.

● We may not succeed in improving existing or developing additional diagnostic products, and, even if we do succeed in developing additional diagnostic products, the diagnostic products may never achieve significant commercial market acceptance.

● The diagnostics market is competitive, and we may not be able to compete successfully, which would adversely impact our ability to generate revenue.

● We are currently offering and developing multiple tests as LDTs and intend to develop and perform LDTs at Aspira Labs in the future. If the FDA were to begin actively regulating our tests, we would incur substantial costs and delays associated with an effort to obtain premarket 510(k) clearance, de novo classification, or premarket approval and incur costs related to compliance with post-market controls.

● Certain of our diagnostic tests and associated software are subject to ongoing regulation by the FDA, and we may develop additional FDA-regulated diagnostic tests and software in the future. Any failure to comply with applicable FDA regulations for our current FDA regulated products, and any delay by or failure of the FDA to authorize future products we submit to the FDA may adversely affect our business, results of operations and financial condition.

● If we or our suppliers fail to comply with FDA requirements for production, marketing and post-market monitoring of our products, we may not be able to market our products and services and may be subject to stringent penalties, product restrictions or recall.

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● If our suppliers fail to produce acceptable or sufficient stock, fail to supply stock due to supply shortages, make changes to the design or labeling of their biomarker kits or discontinue production of existing biomarker kits or instrument platforms, we may be unable to meet market demand for OvaSuite products.

● Changes in healthcare policy could increase our costs and adversely impact sales of and reimbursement for our tests, which would have an adverse effect on our business, financial condition and results of operations.

● We are subject to environmental laws and have potential exposure to environmental liabilities.

● The operation of Aspira Labs requires us to comply with numerous laws and regulations, which is expensive and time-consuming and could adversely affect our business, financial condition and results of operations, and any failure to comply could result in exposure to substantial penalties and other harm to our business.

● Our ability to utilize our net operating loss carryforwards and certain other tax attributes may be limited.

● If we are able to reinstate the ARPA-H award and yet are unable to complete the required milestones under our federal award milestone-based funding agreement, our business, results of operations and financial condition would be adversely affected.

● Failure to renew our Quest Diagnostics agreement could impact our business.

#### Risks Related to Intellectual Property and Product Liability
● If we fail to maintain our rights to utilize intellectual property directed to diagnostic biomarkers, we may not be able to offer diagnostic tests using those biomarkers.

● If a third party infringes on our proprietary rights, we may lose any competitive advantage we have as a result of diversion of our time, enforcement costs and the loss of the exclusivity of our proprietary rights.

● If others successfully assert their proprietary rights against us, we may be precluded from making and selling our products or we may be required to obtain licenses to use their technology.

● Our diagnostic efforts may cause us to have significant product liability exposure.

● Certain of our patent registrations will expire, which may cause us to have significant competition.

#### Operational Risks
● Because our business is highly dependent on key executives and employees, our inability to recruit and retain these people could hinder our business plans.

● Business interruptions could limit our ability to operate our business.

● The operation of Aspira Labs and our Aspira Synergy business depends on the effectiveness and availability of our information systems, including the information systems we use to provide services to our customers and to store employee data. If our information technology systems or those third parties upon which we rely or our data, are or were compromised, we could experience adverse consequences resulting from such compromise, including but not limited to regulatory investigations or actions; litigation; fines and penalties; disruptions of our business operations; reputational harm; loss of revenue or profits; loss of customers or sales; and other adverse consequences.

● We selectively explore acquisition opportunities and strategic alliances relating to other businesses, products or technologies. We may not be successful in integrating other businesses, products or technologies with our

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business. Any such transaction also may not produce the results we anticipate, which could adversely affect our business, financial condition and results of operations.

● Future litigation by or against us could be costly and time-consuming to prosecute or defend.

#### Risks Related to Owning Our Stock
● There is a limited market for our Common Stock.

● If we fail to maintain compliance with the OTC QX minimum listing requirements, our common stock will be subject to removal to the OTC QB market. Our ability to publicly or privately sell equity securities and the liquidity of our common stock could be adversely affected if our common stock is downgraded.

● The liquidity and trading volume of our common stock may be low, and our ownership is concentrated, which could adversely impact the trading price of our common stock and our stockholders' ability to obtain liquidity.

● Our stock price has been, and may continue to be, highly volatile.

● Anti-takeover provisions in our charter, bylaws, other agreements and under Delaware law could make a third-party acquisition of the Company difficult.

● If we raise additional capital in the future, your ownership in us could be diluted.

● Sales of a substantial number of our shares of common stock in the public market could cause our stock price to fall.

● Because we do not currently intend to declare cash dividends on our shares of common stock in the foreseeable future, stockholders must rely on appreciation of the value of our common stock for any return on their investment.

● The exercise of our outstanding options and warrants will dilute stockholders and could decrease our stock price.

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#### PART I

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| | |
|:---|:---|
| **ITEM 1.** | **BUSINESS** |

---

#### Company Overview
Aspira Women's Health Inc. is dedicated to the discovery, development, and commercialization of noninvasive, AI-powered tests to aid in the diagnosis of gynecologic diseases, starting with ovarian cancer.

We plan to broaden our focus to the differential diagnosis of other gynecologic diseases that typically cannot be assessed through traditional non-invasive clinical procedures. We expect to continue commercializing our existing and new technology through both in-house and distributed pathways. We also intend to continue to raise public awareness regarding the diagnostic superiority of Ova1Plus as compared to cancer antigen 125 ("CA-125") on its own for all women with adnexal masses, as well as the performance of our tests in detecting ovarian cancer risk in different racial populations. We plan to continue to expand access to our tests among Medicaid patients as part of our corporate mission to make the best care available to all women, and we plan to advocate for legislation and the adoption of our technology in professional society guidelines to provide broad access to our products and services.

We expect our extensive experience with gynecologists and healthcare providers, along with the historical adoption of our OvaSuite tests, to continue to drive growth as we introduce new products. We believe our ability to successfully develop novel AI-enabled assays is superior to others based on our know-how and extensive experience in designing and successfully launching FDA-approved and laboratory developed blood tests to aid in the diagnosis of ovarian cancer. Moreover, our history of successfully collaborating with world-class research and academic institutions allows us to innovate and provide outstanding patient care.

We own and operate Aspira Labs, Inc., a research and commercial Clinical Laboratory Improvement Amendments of 1988 certified ("CLIA") laboratory in Texas.

Our product pipeline is focused on two areas: endometriosis and ovarian cancer.

In endometriosis, we are developing and intend to introduce a new non-invasive test to aid in the diagnosis of this debilitating disease that impacts millions of women worldwide. Our ENDOinform program is focused on developing a multi-marker test that combines serum proteins, micro RNA ("miRNA") and clinical data (metadata) for the identification of endometriosis.

Our endometriosis portfolio reaches a larger addressable market than our ovarian cancer portfolio. According to the U.S. Office on Women's Health, endometriosis affects approximately 10% of reproductive age women, representing more than 6.6 million women in the United States. We believe the proliferation of commercially available and in-development therapeutics for the treatment of endometriosis will create a significant demand for a non-invasive diagnostic.

In ovarian cancer, we have developed clinical data to support the use of our OvaWatch test multiple times for the monitoring of an adnexal mass. In the second quarter of 2024, we expanded the features of our commercially available OvaWatch test for monitoring of adnexal masses through periodic testing at physician prescribed intervals, marking the successful completion of the vision for OvaSuite. The successful expansion of the OvaWatch mass monitoring feature in the second quarter of 2024 results in an addressable market for our tests of between 2 and 4 million tests per year, based on 1.5 million women presenting with an indeterminate adnexal mass.

Our OVAinform development program continues to progress. OVAinform is a multi-marker test that combines serum proteins, miRNA and clinical data (metadata) for assessing the risk of ovarian cancer. We believe there is utility in expanding the OVAinform indication to include asymptomatic women at elevated risk due to genetic risk or first degree relatives with ovarian cancer. The inclusion of this cohort in our indication will increase the addressable market to include another 2.5 million women, increasing the total to 4 million women.

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#### Recent developments:
Our current capital resources are not sufficient to fund our operations for the remainder of 2026. Accordingly, we have been pursuing strategic alternatives and seeking to raise additional capital.

On March 5, 2025, we entered into a securities purchase agreement with certain existing accredited shareholders ("the "Purchasers") for the issuance and sale in a private placement (the "March 2025 Private Placement") of an aggregate principal amount of approximately $1,365,000 in the form of Senior Secured Convertible Promissory Notes (the "Convertible Notes").

The Convertible Notes, which were convertible into units consisting of one share of common stock and 2.25 warrants (the "March 2025 Warrants"), were senior, secured obligations of the Company. Interest accrued and was payable on a quarterly basis in kind at 3.34%, the applicable federal rate at the time of the transaction. The Convertible Notes would mature on March 6, 2030, unless earlier converted in accordance with the terms of the Convertible Notes. In addition, the Company had the option to convert the Convertible Notes into units at the Conversion Price if the sum of the net proceeds from the sale of the Convertible Notes and the net proceeds from the sale of any shares of common stock and warrants by the Company subsequent to the March 2025 Private Placement equaled or exceeded $4 million. The Company exercised that option on March 11, 2025 and converted 100% of the Convertible Notes into Units.

On April 15, 2025, we received written notice from the Office of General Counsel of The Nasdaq Stock Market ("Nasdaq") indicating that the Nasdaq Hearings Panel determined to delist our shares from Nasdaq due to our failure to meet Nasdaq's continued listing standard under Nasdaq Listing Rule 5550(b)(1). Our common stock was traded on the OTC Markets Group effective at the open of trading on April 17, 2025 under the symbol "AWHL." The Company's common stock is currently traded on the OTC QX Best Market.

On June 9, 2025, we received a notice from Advanced Research Projects Agency for Health ("ARPA-H") that our ENDOinform contract originally signed in October 2024 was terminated for failure to meet the specifications of the third milestone under the contract. We received $2,000,000 in the fourth quarter of 2024 and $1,500,000 in the first quarter of 2025 prior to the termination of the contract. The loss of this non-dilutive funding eliminates a planned source of support for development activities and may delay the timeline to commercialize ENDOinform unless we are able to reinstate the award or secure alternative financing.

On September 19, 2025, the Company entered into an amendment to the Securities Purchase Agreement, pursuant to which, among other things, the Company issued Amended and Restated Series A Common Stock Warrants and amended and restated the March 2025 Warrants to provide for a fixed exercise price of $0.35 per share, eliminated the cashless exercise feature so that all exercises must be paid in cash, extended the term of the warrants from five to six years from the issuance date, and changed the initial exercisability from five months to six months after the issuance date. Prior to this amendment, the March 2025 Warrants were exercisable for five years at $0.25 per share for the first 24 months after issuance, and $0.50 per share thereafter.

The participants in the March 2025 Private Placement are entitled to three representatives on our board of directors. Each of the three seats has been filled.

#### Scientific Basis for Our Products:
Science of Biomarkers: Our focus on translational biomarkers and informatics enables us to address the market for novel diagnostic tests that simultaneously measure multiple biomarkers. A biomarker is a biomolecule or variant biomolecule (e.g., DNA, RNA or protein) that is present at measurably greater or lesser concentrations, or is present in an altered form, in a disease state versus a normal condition. Conventional protein tests measure a single protein biomarker whereas most diseases are complex. We believe cancer and other complex diseases are heterogeneous at the causative level (i.e., most diseases can be traced to multiple potential etiologies) and at the human response level (i.e., each individual afflicted with a given disease can respond to that ailment in a specific manner). Protein biomarkers (our entrenched

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technology), miRNA molecular biomarkers and metadata (age, body mass index, etc.) each provide independent and non-overlapping evidence for a disease state. This increases the accuracy, sensitivity and specificity of the test in most cases.

Consequently, measuring a single biomarker when multiple biomarkers may be altered in a complex disease is unlikely to provide meaningful information about the disease state.

We believe that our approach of monitoring and combining multiple biomarkers using a variety of analytical techniques has allowed and will continue to allow us to create diagnostic tools that provide information about the disease state with sufficient sensitivity and specificity to aid the physician considering treatment options for patients with complex diseases. Such assays are sometimes referred to as Multivariate Index Assays ("MIAs") and often utilize advanced algorithms based on logistic regression, pattern recognition and the like. Often, MIA algorithms are non-intuitive and; therefore, require rigorous clinical validation and error modeling. Aspira and its collaborators are considered experts in these areas and, in the case of Ova1 and Overa, presented both the clinical validation and error modeling needed to gain pre-market authorization from the FDA. In the case of Ova1, the FDA granted a request for *de novo* classification of an ovarian adnexal mass assessment score test system, a type of in vitro diagnostic device; in the case of Overa, FDA granted a 510(k) clearance.

#### Our Business and Products:
We currently commercialize the following blood test products and related services:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(1) the Ova1Plus workflow, which uses Ova1 as the primary test and Overa as a reflex for Ova1 intermediate range results. Ova1 is a qualitative serum test intended as an aid to further assess the likelihood of malignancy in women with an ovarian adnexal mass for which surgery is planned when the physician's independent clinical and radiological evaluation does not indicate malignancy. Overa is a second-generation biomarker test intended to maintain Ova1's high sensitivity while improving specificity. The Ova1Plus workflow leverages the strengths of Ova1's MIA sensitivity and Overa's (MIA2G) specificity to increase performance; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(2) OvaWatch, which is intended to assist in the initial and periodic clinical assessment of malignancy risk in all women thought to have an indeterminate or benign adnexal mass.

Our products are distributed through our direct national sales force, including field sales and inside sales. OvaSuite tests are marketed to both physicians via a direct referral to Aspira as well as to laboratories via our Aspira Synergy platform. We also engage in channel distribution partnerships by which OvaSuite is distributed via laboratory partners such as BioReference Health, LLC. ("BioReference") and Associated Regional University Pathologists ("ARUP"). In December 2025, we executed an agreement with Mayo Clinical Laboratories ("Mayo") as a new channel partner for Ova1 and OvaWatch distribution.

Our Ova1 test received FDA *de novo* classification in September 2009. Ova1 comprises instruments, assays, reagents, and the OvaCalc software, which includes a proprietary algorithm that produces a risk score. Our Overa test, which includes an updated version of OvaCalc, received FDA 510(k) clearance in March 2016. Ova1, Overa and OvaWatch each use the Roche Cobas 4000, 6000 and 8000 platforms for analysis of proteins. Revenue from Ova1 and OvaWatch is included in the results of operations in total revenue for the year ended December 31, 2025.

OvaWatch has been developed and is validated for use in Aspira's CLIA-certified lab as a non-invasive blood-based risk assessment test for use in conjunction with clinical assessment and imaging to determine ovarian cancer risk for patients with an adnexal mass whose adnexal mass has been determined by an initial clinical assessment as indeterminate or benign. OvaWatch is the only commercially available blood test available for assessment of the risk of ovarian cancer in women diagnosed with an adnexal mass considered indeterminate or benign by a physician's preliminary clinical assessment.

We collected clinical data to support the utility of OvaWatch to aid in surgical referral and as a longitudinal monitoring test, resulting in two manuscripts that were published in peer review journals in May 2024. In addition, an

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abstract highlighting data evaluating the use of OvaWatch to assess ovarian cancer risk in pre- and post-menopausal women was presented at the Annual Meeting of The Menopause Society in September 2024.

We own and operate Aspira Labs, based in Austin, Texas, a Clinical Chemistry and Endocrinology Laboratory accredited by the College of American Pathologists, which specializes in applying biomarker-based technologies to address critical needs in the management of gynecologic cancers and disease. Aspira Labs provides expert diagnostic services using a state-of-the-art biomarker-based risk assessment to aid in clinical decision making and to advance personalized treatment plans. The lab currently performs our Ova1Plus workflow and OvaWatch testing, and we plan to expand the testing to other gynecologic conditions with high unmet needs. Aspira Labs holds a CLIA Certificate of Accreditation and a state laboratory license in California, Maryland, New York, Pennsylvania and Rhode Island. The Centers for Medicare & Medicaid Services ("CMS") issued a supplier number to Aspira Labs in 2015. Aspira Labs also hold a current ISO 13485 certification which is the most accepted standard worldwide for medical device.

In the United States, revenue for diagnostic tests comes from several sources, including third-party payers such as insurance companies, government healthcare programs, such as Medicare and Medicaid, client bill accounts and patients. Novitas Solutions, a Medicare Administrative Carrier, covers and reimburses for Ova1 tests performed in certain states, including Texas. Due to our billed Ova1 tests being performed exclusively at Aspira Labs in Texas, the Local Coverage Determination ("LCD") from Novitas Solutions provides national coverage for patients enrolled in Medicare as well as Medicare Advantage health plans. We have applied for an LCD for OvaWatch, which is currently under review.

In November 2016, the American College of Obstetricians and Gynecologists ("ACOG") issued Practice Bulletin Number 174 which included Ova1, defined as the "Multivariate Index Assay", outlining ACOG's clinical management guidelines for adnexal mass management. Practice Bulletin Number 174 recommends that obstetricians and gynecologists evaluating women with adnexal masses who do not meet Level A criteria of a low-risk transvaginal ultrasound should proceed with Level B clinical guidelines. Level B guidelines state that the physician may use risk assessment tools such as existing CA-125 technology or Ova1 ("Multivariate Index Assay") as listed in the bulletin. Based on this, Ova1 achieved parity with CA-125 as an ACOG Level B clinical recommendation for the management of adnexal masses.

Practice Bulletins summarize current information on techniques and clinical management issues for the practice of obstetrics and gynecology. Practice Bulletins are evidence-based documents, and recommendations are based on the evidence. This is also the only clinical management tool used for adnexal masses. Although there are Practice Bulletins, guidelines do not exist for adnexal masses. ACOG guidelines do exist, however, for ovarian cancer management.

#### Studies and Publications
On May 7, 2024, we announced the publication of two peer-reviewed manuscripts. The first manuscript, entitled "*Ovarian Cancer Surgical Consideration is Markedly Improved by the Neural Network Powered-MIA3G Multivariate Index Assay*" was published in the peer-reviewed journal Frontiers of Medicine on May 2, 2024. The findings of this study demonstrate that use of OvaWatch to stratify risk in patients with an adnexal mass might help to reduce surgical backlogs and unnecessary surgical referrals. The second manuscript, entitled "*Neural Network-derived Multivariate Index Assay Demonstrates Effective Clinical Performance in Longitudinal Monitoring of Ovarian Cancer Risk*" was published in the journal Gynecologic Oncology on May 3, 2024. The findings of this study demonstrate that OvaWatch could be an effective tool for the monitoring of ovarian cancer risk over time in women with indeterminate or low risk adnexal masses. Based on common practice for adnexal mass management and consistent with the study, OvaWatch can be drawn by the provider every three to six months for active surveillance of an adnexal mass.

A publication entitled *Serum miRNA improves the accuracy of a multivariate index assay for triage of an adnexal mass* was published in the journal *Gynecologic Oncology,* August of 2024 from the laboratory of our collaborator Dr. Kevin Elias, formerly affiliated with the Brigham and Women's Hospital. The paper describes the novel combination of miRNAs and serum proteins to achieve increased performance in the assessment of malignancy risk in patients with an adnexal mass. The miRNAs, discovered by Dr Elias' team, in combination with serum proteins from Aspira Women's Health's proprietary multivariate index assays Ova1 and Overa showed increased sensitivity for detection of malignancy and broader detection of diverse ovarian cancer subtypes. This publication establishes the feasibility of improved tests using multi-omic information. (*Webber JW, Wollborn L, Mishra S, Vitonis AF, Cramer DW, Phan RT, Pappas TC, Stawiski* 

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*K, Fendler W, Chowdhury D, Elias KM. Serum miRNA improves the accuracy of a multivariate index assay for triage of an adnexal mass. Gynecol Oncol. 2024 Aug 23;190:124-130*.)

An abstract entitled "*Application of a Deep Neural Network-Based Algorithm to Provide Additional Information in the Assessment of Adnexal Masses Classified as Indeterminate by Imaging*" was presented as a poster at the Annual Meeting of The Menopause Society in September 2024. This presentation highlighted data evaluating the use of OvaWatch to assess ovarian cancer risk in pre- and post-menopausal women. The data demonstrated that in women with an adnexal mass and an indeterminate ultrasound imaging result, the OvaWatch result indicated low malignant potential of the mass in more than 70% of patients. The use of OvaWatch could provide additional information to reduce surgical referrals.

An EndoCheck-related abstract entitled "*Association of the Endometriosis Health Profile5 (EHP5) with Non-Invasive Biomarkers in Patients with Suspected Endometriosis*" was presented as a poster at the 27<sup>th</sup> Annual National Association of Nurse Practitioner's in Women's Health Women's Healthcare Conference in September 2024. This poster examined the association of biomarkers for ovarian endometriosis (endometrioma) with quality-of-life survey responses before and after surgical intervention. There was no association between endometrioma biomarkers and self-reported patient quality of life either prior to or after surgery, and this was consistent with other research.

An EndoCheck-related virtual poster entitled "*A Proprietary Protein-Based Algorithm May Increase Sensitivity of Endometrioma Detection When Combined with Imaging*" was presented at the annual meeting of the American Association of Gynecologic Laparoscopists in November 2024. This poster summarizes a preliminary study on the performance of imaging combined with a protein biomarker-based algorithm. The combination of these diagnostic tools resulted in increased sensitivity of detection of endometrioma and could be effective in risk assessment and surgical planning for this condition.

Aspira's observational clinical study entitled *Deep Neural Network Stratification for Use Detecting Endometriosis in Women Affected by Chronic Pelvic Pain (EndoCheck)* (https://clinicaltrials.gov/study/NCT05245695) continued brisk enrollment through 2025 and will conclude in 2026. This study collects serum, plasma and circulating cells from women 14-50 years old who have symptoms consistent with endometriosis and are schedule to undergo diagnostic surgery for suspicion of endometriosis. This intended-use dataset is being used to develop and validate ENDOinform.

#### The Diagnostic Field
The economics of healthcare demand effective and efficient allocation of resources which can be accomplished through disease prevention, early detection of disease leading to early intervention, and diagnostic tools that can triage patients to more appropriate therapy and intervention. In 2026, Fortune Business Insight, a market research and business consulting partnership, published a study which forecasts the global *in vitro diagnostic* ("IVD") market to reach $135.65 billion by 2034, growing at a compound annual growth rate of 6.5% from 2026 to 2034. We have chosen to concentrate our business focus in the areas of gynecologic oncology and disease where we have established strong key opinion leaders, and provider and patient relationships. Demographic trends suggest that, as the population ages, the burden from gynecologic diseases will increase and the demand for quality diagnostic, prognostic and predictive tests will escalate. In addition, the areas of gynecologic oncology and disease generally lack quality diagnostic tests and, therefore, we believe patient outcomes can be significantly improved by the development of novel diagnostic tests. Furthermore, an increasing number of women are becoming aware of the importance of early detection, particularly in gynecologic diseases.

#### Ovarian Cancer Background
Commonly known as the "silent killer," ovarian cancer leads to over 12,000 deaths each year in the United States. The American Cancer Society estimates that there will be over 21,000 new ovarian cancer cases diagnosed in 2026, with many patients diagnosed in the late stages of the disease in which the cancer has spread beyond the ovary. Unfortunately, ovarian cancer patients in the late stages of the disease have a poor prognosis, which leads to high mortality rates. According to the 2023 Cancer Annual Report by Woman's Hospital, when ovarian cancer is diagnosed at its earliest stage (stage 1), patients have up to a 93%, 5-year survival rate following surgery and/or chemotherapy. The 5-year survival rate falls to as low as 31% for ovarian cancer patients diagnosed in the late-stages of the disease.

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While the diagnosis of ovarian cancer in its earliest stages greatly increases the likelihood of long-term survival from the disease, another factor that predicts clinical outcomes from ovarian cancer is the specialized training of the surgeon who operates on the ovarian cancer patient. Numerous studies have demonstrated that treatment of malignant ovarian tumors by specialists such as gynecologic oncologists coupled with specialist medical centers improves outcomes for women with these tumors. Published guidelines from the SGO and the ACOG recommends referral of women with malignant ovarian tumors to specialists. Accordingly, there is a clinical need for a diagnostic test that can provide adequate predictive value to stratify patients with a pelvic mass into those with a high-risk of invasive ovarian cancer versus those with a low risk of ovarian cancer, which is essential for improving overall survival in patients with ovarian cancer. The goal is to catch the mass early before it becomes late-stage cancer.

Although adnexal masses are relatively common, malignant tumors are less so. Studies have indicated that the prevalence of simple ovarian cysts in women 55 years of age and older can be as high as 14%. Adnexal masses are thought to be even more common in premenopausal women. For instance, a University of Kentucky ovarian cancer screening study found that the rate of postmenopausal women with persistently abnormal ultrasound findings requiring surgery was 1.4%. According to 2020 U.S. census data, there are 42.6 million women between the ages of 50 and 70 in the U.S., suggesting that there are nearly 600,000 suspicious adnexal masses in this segment alone. When managing an adnexal mass, physicians will either take a surgical management approach or a clinical management approach. Patients that do require surgical management could potentially benefit from the use of the Ova1Plus workflow. Patients not referred for surgical intervention may benefit from the use of OvaWatch to confirm the low risk of malignancy of a mass that was determined to be indeterminate or benign by initial clinical assessment.

The ACOG Ovarian Cancer Guidelines and the SGO guidelines help physicians evaluate adnexal masses for malignancy. These guidelines take into account menopausal status, CA125 levels, and physical and imaging findings. However, these guidelines have notable shortcomings because of their reliance on diagnostics with certain weaknesses. Most notably, studies have shown that the CA125 blood test, which is cleared by the FDA for the monitoring for recurrence of ovarian cancer only, is negative in up to 31% of early-stage ovarian cancer cases. Moreover, CA125 can be elevated in numerous conditions and diseases other than ovarian cancer, including menstruation, benign ovarian masses, liver disease, endometriosis, pelvic inflammatory disease, pregnancy and uterine fibroids.

These shortcomings limit the CA125 blood test's utility in distinguishing benign from malignant ovarian tumors or for use in detection of early-stage ovarian cancer.

Transvaginal ultrasound is another diagnostic modality used with patients with ovarian masses. Attempts at defining specific morphological criteria that can aid in a benign versus malignant diagnosis have led to the morphology index and the risk of malignancy index, with reports of 40-70% predictive value. However, ultrasound interpretation can be variable and dependent on the experience of the operator. Accordingly, the ACOG and SGO guidelines indicate that transvaginal ultrasounds are rarely conclusive in identifying early-stage ovarian cancer and malignancy in pre-menopausal women. Efforts to improve detection of cancer by lowering the cutoff for CA125 (the "Modified ACOG/SGO Guidelines") provide only a modest benefit, since CA125 is absent in about 20% of epithelial ovarian cancer cases and is poorly detected in early-stage ovarian cancer overall.

ACOG practice bulletin 174 (November 2016) states the following regarding our Ova1-branded product "The multivariate index assay has demonstrated higher sensitivity and negative predictive value for ovarian malignancy when compared with clinical impression and CA 125 alone."

The ovarian cancer information page on American Cancer Society's website indicates that:

For women who have an ovarian tumor, a test called Ova1 can measure the levels of 5 proteins in the blood. The levels of these proteins, when looked at together, are used to determine whether a woman's tumor should be considered low-risk or high-risk. If the tumor is labeled 'low-risk' based on this test, the woman is not likely to have cancer. If the tumor is considered 'high-risk,' the woman is more likely to have a cancer and should see a specialist (a gynecologic oncologist). This test is NOT a screening test and it is NOT a test to decide if you should have surgery or not- it is meant for women who have an ovarian tumor where surgery has been decided but have not yet been referred to a gynecologic oncologist.

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Aspira is committed to developing diagnostic tools for women of all ages, races and ethnicities. In 2019, two studies were released indicating superior clinical performance of Ova1 over CA125 and Ova1 over CA125, HE4 and Risk of Ovarian Malignancy Algorithm ("ROMA") in African American women. In 2022, another study was released indicating superior clinical performance of Ova1 over CA125 in Filipino women.

#### Commercialization and Distribution
We market and distribute our products through both in-house and distributed pathways.

Starting in 2014, we offered Ova1 via Aspira Labs. In March 2015, we entered into a commercial agreement with Quest Diagnostics. Pursuant to this agreement, as subsequently amended, all Ova1 U.S. testing services for Quest Diagnostics customers were transferred to Aspira's wholly-owned subsidiary, Aspira Labs and Quest Diagnostics has continued to provide blood draw and logistics support by transporting specimens from its clients to Aspira Labs for testing in exchange for a market value fee. In 2015, a laboratory reference agreement for Ova1 was executed between Aspira and ARUP. This agreement serves to provide health system and commercial laboratories, who are clients of ARUP, with access to Ova1 via their existing laboratory channels. In 2022, the agreement was amended to include OvaWatch testing services. In October 2022, we launched a co-marketing and distribution collaboration with BioReference, as a new channel for volume growth. Under terms of the agreement, the Aspira and BioReference and sales teams collaborate to sell Ova1Plus to gynecologists and other women's healthcare providers nationwide. In November 2024, Aspira and BioReference announced the expansion of the sale team collaboration to include OvaWatch.

Cleveland Clinic Foundation ("Cleveland Clinic") and Mayo are two of the preeminent research laboratories in the world. In August 2025, after integrating Ova1 into its care pathway, Cleveland Clinic issued a newsletter reporting that use of Ova1 improved gynecologic oncology referral patterns to increase personalized surgical management based on patient risk stratification. In December 2025, we executed an agreement with Mayo as a new channel partner for Ova1 and OvaWatch distribution. This partnership with Mayo will help us gain access to Mayo health system clients nationally. Under the terms of the agreement, we will work with the Mayo sales team to collaborate and co-market to Mayo clients beginning in 2026.

#### Customers
In the United States, our clinical customer base includes physicians, physician office laboratories and national and regional laboratories. Both within and outside the United States, our customer specimens are sent directly to us, and we either bill third party payers or bill clients through client bill arrangements. We also offer access to our Ova1 and Overa assays via our decentralized technology transfer relationships established between us and authorized distributors.

#### Research and Development
Our research and development efforts center on the discovery and validation of biomarkers and the combinations of biomarkers with other "omics" that can be developed into diagnostic assays. We have done this predominantly through collaborations we have established with academic institutions such as the Johns Hopkins University School of Medicine, the University of Texas, M.D. Anderson Cancer Center, Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital and Medical University of Lodz. In addition, we actively seek collaborations and initiate dialog with clinical academics and other organizations, in order to generate publications, intellectual property or test development in broader areas of gynecologic oncology and other gynecologic diseases.

Our research and development efforts are detailed in the "Product Pipeline" section in Item 7, *Management's Discussion and Analysis.*

#### Commercial Operations
We have a commercial infrastructure, including sales and marketing and reimbursement expertise. We also operate Aspira Labs, a CLIA certified clinical laboratory in Austin, Texas. Our sales representatives work to identify opportunities for educating general gynecologists and gynecologic oncologists on the benefits of Ova1 and OvaWatch. In

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February 2015, Aspira received ISO 13485 (currently ISO 13485:2016) certification for our quality management system from the British Standards Institution (BSI), one of the world's leading certification bodies. We currently hold CE marks for Ova1 and Overa .

22,515 OvaSuite tests were performed in 2025 compared with 24,305 in 2024. In 2025, we conducted a comprehensive review of the commercial operations, identifying those provider relationships that have historically been unable to deliver appropriate reimbursement. The review resulted in an intended selective reduction in test volumes as compared to 2024. The commercial team continues to focus on driving physician adoption that aligns with profitable payer reimbursement. This targeted approach is driven by payer claims data that gives visibility into payer mix and utilization trends. The commercial team works with local key opinion leaders and meets with medical directors to discuss the clinical need, our technology solutions package and case studies showing positive outcomes using OvaSuite.

We believe OvaWatch will continue to have a significant impact on the ordering behavior of physicians with respect to our ovarian cancer blood tests. OvaWatch was developed for use with women with adnexal masses that have been identified as either benign or indeterminate through initial clinical assessment. It is estimated that physicians see more than three times as many women with benign or indeterminate masses compared to women with masses that are planned for surgery. In addition, we believe that the OvaWatch longitudinal monitoring test could further increase the ordering frequency of our ovarian cancer blood tests.

#### Revenue and Reimbursement
In the United States, revenue for diagnostic tests comes from several sources, including third-party payers such as insurance companies, government healthcare programs, such as Medicare and Medicaid, client bill accounts and patients. Ova1 and OvaWatch are performed at Aspira Labs in Austin, TX. Novitas Solutions is our Medicare Administrative Contractor (a "MAC"). All Medicare claims nationally for Ova1 and OvaWatch are billed via the Novitas MAC in accordance with CMS guidelines. Novitas covers both Ova1 and OvaWatch and has an LCD for Ova1 under LCD L35396 – Biomarkers for Oncology. Aspira Labs also bills third-party commercial and other government payers as well as client bill accounts and patients for Ova1 and OvaWatch. Through December 31, 2025, Aspira's product and related services revenue was primarily limited to revenue generated by sales of Ova1 and OvaWatch.

In December 2013, the CMS made its final determination and authorized Medicare contractors to set prices for Multianalyte Assays with Algorithmic Analyses ("MAAA") test CPT codes when they determine it is payable. In late 2016, Ova1 was included on the list of clinical diagnostic laboratory test procedure codes as one for which the CMS would require reporting of private payer rates as part of the implementation of Protecting Access to Medicare Act of 2014 ("PAMA"). In November 2017, we announced that the CMS released the Final 2018 Clinical Laboratory Fee Schedule ("CLFS"), effective January 1, 2018. Under the fee schedule, the price for Ova1 (CPT code 81503) is $897. This rate was based on the median of private-payer payments submitted to CMS by companies, including Aspira Labs, as part of the market-based payment reform mandated through PAMA. The rate was scheduled to be in effect for a three-year term from January 2018 through December 2020. This rate is now extended through 2025. In 2024 and 2026, CMS announced that it would continue to delay the period during which rates would be evaluated. Therefore, we will not be responsible for providing reimbursement rates until May 2026. There are no assurances that reimbursement rates will not be changed.

We have a comprehensive reimbursement plan for Ova1 and OvaWatch, and have targeted third-party payers, Medicare, Medicare Advantage, State Medicaid and Managed Medicaid plans for coverage and reimbursement. In April 2023 we began billing OvaWatch with our newly awarded Proprietary Laboratory Analyses ("PLA Code") 0375U. Since we began billing OvaWatch with the PLA Code, our reimbursement has been more in-line with historical Ova1Plus experience, resulting in the OvaWatch average unit price ("AUP") of $394 for the year ended December 31, 2025.

Ova1 is considered medically necessary in the Lab Management Guidelines for one of the largest lab benefit management companies who works with payers to ensure adherence to clinical guidelines. We continue to make gains toward reimbursement for OvaWatch as we build clinical evidence to support coverage.

In February 2023, we signed a contract with a national commercial payer which provides patient coverage for Ova1 and OvaWatch beginning in April 2023. Further, CMS approved the crosswalk of the fee to be paid for OvaWatch

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to the fee paid for Ova1. Effective January 1, 2024, we have been reimbursed at a rate of $897 for all OvaWatch and Ova1 tests processed for Medicare patients meeting applicable coverage requirements.

In addition, the United States and some foreign jurisdictions are considering or have enacted a number of legislative and regulatory proposals to change the healthcare system in ways that could affect our ability to sell our products profitably. Among policy makers and payers in the United States and elsewhere, there is significant interest in promoting changes in healthcare systems with the stated goals of containing healthcare costs, improving quality or expanding access.

Further, other legislative changes have been proposed and adopted since the Affordable Care Act was enacted. For example, the Budget Control Act of 2011, among other things, included reductions to CMS payments to providers of 2% per fiscal year, which went into effect on April 1, 2013 and, due to subsequent legislative amendments to the statute, will remain in effect until 2032 unless additional congressional action is taken.

The Nancy Gardner Sewell Medicare Multi-Cancer Early Detection Screening Coverage Act, enacted on February 3, 2026, modernizes the Medicare program by creating a benefit category for multi-cancer early detection ("MCED") tests, which enables CMS to initiate an evidence-based coverage process for multi-cancer tests upon approval of the tests by the Food and Drug Administration ("FDA"). The law takes effect in 2028.

There may be additional health reform initiatives by legislators at both the federal and state levels, regulators and third-party payers to reduce costs while expanding individual healthcare benefits. Certain of these changes could impose additional limitations on the rates we will be able to charge for our current and future products or the amounts of reimbursement available for our current and future products from governmental agencies or third-party payers.

Biomarker legislation continues to gain momentum at the state level with many states enacting legislation requiring coverage in both public and private insurance plans. Additionally, more states are evaluating legislation and have introduced biomarker access bills in 2026.

#### Competition
The diagnostics industry in which we operate is competitive and evolving. There is intense competition among healthcare, biotechnology and diagnostics companies attempting to discover candidates for potential new diagnostic products. These companies may:

● develop new diagnostic products in advance of us or our collaborators;

● develop diagnostic products that are more effective or cost-effective than those developed by us or our collaborators;

● obtain regulatory clearance or approval of their diagnostic products more rapidly than us or our collaborators; or

● obtain patent protection or other intellectual property rights that would limit our or our collaborators' ability to develop and commercialize, or a customers' ability to use our or our collaborators' diagnostic products.

We compete with companies in the United States and abroad that are engaged in the development and commercialization of novel biomarkers that may form the basis of novel diagnostic tests. These companies may develop products that are competitive with and/or perform the same or similar functions as the products offered by us or our collaborators, such as biomarker specific reagents or diagnostic test kits. Also, clinical laboratories may offer testing services that are competitive with the products sold by us or our collaborators. For example, a clinical laboratory can either use reagents purchased from manufacturers other than us or use its own internally developed reagents to make diagnostic tests. If clinical laboratories make tests in this manner for a particular disease, they could offer testing services for that disease as an alternative to products sold by us used to test for the same disease. The testing services offered by clinical laboratories may be easier to develop and market than test kits developed by us or our collaborators because the testing

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services are not subject to the same clinical validation requirements that are applicable to FDA-cleared or approved diagnostic test kits.

Fujirebio Diagnostics developed ROMA. ROMA combines two tumor markers and menopausal status into a numerical score using a publicly available algorithm. This test has the same intended use and precautions as Ova1. ROMA is currently marketed as having utility limited to epithelial ovarian cancers, which accounts for 80% of ovarian malignancies. Based upon the results of studies done in 2013 and 2019, we believe that Ova1 has superior sensitivity when compared to the Fujirebio Diagnostics test.

In addition, competitors such as AOA Dx, ClearNote Health, Cleo Diagnostics and Mercy BioAnalytics, and others have publicly disclosed that they have been or are currently working on ovarian cancer diagnostic assays. Exact Sciences, Grail and others are working on MCED tests that include ovarian cancer detection. Academic institutions periodically report new findings in ovarian cancer diagnostics that may have commercial value.

A number of diagnostic and academic organizations have announced plans or published studies related to the development of a non-invasive diagnostic tool for the identification of endometriosis. If successful, the product may be competitive with our endometriosis offerings. Competitors include, but are not limited to, Afynia, DotLab, Endodiag, HERA Biotech Heranova, Proteomics International and Ziwig. We believe our experience developing and refining complex multi-omic clinical assays specifically targeting gynecologic diseases and pelvic masses, coupled with our experienced women's health field sales team, are significant competitive advantages in bringing new diagnostic tests to market.

#### Intellectual Property Protection
Our intellectual property includes federally registered trademarks and service marks as well as federally pending trademark and service mark applications for our product and service offerings, and a portfolio of owned, co-owned or licensed patents and patent applications. As of the date of the filing of the Form 10-K, our clinical diagnostics patent portfolio included 14 issued United States patents, 8 pending United States patent applications and numerous pending patent applications and issued patents outside the United States. These patents and patent applications are directed to diagnostic technologies.

#### Manufacturing
We are the manufacturer of FDA cleared products Ova1 and Overa, which are part of the Ova1Plus workflow. We also perform OvaWatch as an LDT. The component assays use purchased reagents. Because we do not directly manufacture the component assays, we are required to maintain supply agreements with manufacturers of each of the assays. As part of our quality systems, reagent lots for these assays are tested to ensure they meet specifications required for inclusion. Only reagent lots determined by us as having met these specifications are permitted for use in our testing. Our principal supplier for the component reagents is Roche Diagnostics Corporation.

#### Environmental Matters

#### Medical Waste
We are subject to licensing and regulation under federal, state and local laws relating to the handling and disposal of medical specimens and hazardous waste as well as relating to the safety and health of laboratory employees. Aspira Labs is operated in material compliance with applicable federal and state laws and regulations relating to disposal of all laboratory specimens. We utilize outside vendors for disposal of specimens. We cannot eliminate the risk of accidental contamination or discharge and any resultant injury from these materials. Federal, state and local laws and regulations govern the use, manufacture, storage, handling and disposal of these materials. We could be subject to fines, penalties and damages claims in the event of an improper or unauthorized release of, or exposure of individuals to, hazardous materials. In addition, claimants may sue us for injury or contamination that results from our use, or the use by third parties, of these materials, and our liability may exceed our total assets. Compliance with environmental laws and regulations is expensive, and current or future environmental regulations may impair our research, development or production efforts.

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#### Occupational Safety
In addition to its comprehensive regulation of safety in the workplace, the Federal Occupational Safety and Health Administration has established extensive requirements relating to workplace safety for healthcare employers whose workers may be exposed to blood-borne pathogens such as HIV and the hepatitis virus. These regulations, among other things, require work practice controls, protective clothing and equipment, training, medical follow-up, vaccinations and other measures designed to minimize exposure to chemicals and transmission of blood-borne and airborne pathogens. Although we believe that we have complied in all material respects with such federal, state and local laws, failure to comply could subject us to denial of the right to conduct business, fines, criminal penalties and other enforcement actions.

#### Specimen Transportation
Regulations of the Department of Transportation, the International Air Transportation Agency, the Public Health Service and the Postal Service apply to the surface and air transportation of clinical laboratory specimens. Although we believe that we have complied in all material respects with such federal, state and local laws, failure to comply could subject us to denial of the right to conduct business, fines, criminal penalties and other enforcement actions.

#### Government Regulation

#### FDA Regulation of Medical Devices
In the U.S., medical devices, including IVD products ("IVDs"), are subject to extensive regulation by the FDA, under the Federal Food, Drug, and Cosmetic Act (the "FDC Act"), and its implementing regulations, and certain other federal and state statutes and regulations. The laws and regulations govern, among other things, the design, manufacture, storage, recordkeeping, approval, labeling, promotion, post-approval monitoring and reporting, distribution and import and export of medical devices, including IVDs. IVDs are a type of medical device and include reagents and instruments used in the diagnosis or detection of diseases or conditions. Predictive, prognostic, and screening tests can also be IVDs. Failure to comply with applicable requirements may subject a device and/or its manufacturer to a variety of administrative and judicial sanctions, such as FDA refusal to approve pending applications for pre-market approval ("PMAs") or other applications, issuance of warning letters or untitled letters, mandatory product recalls, import detentions, civil monetary penalties, and/or judicial sanctions, such as product seizures, injunctions, and criminal prosecution.

The FDC Act classifies medical devices into one of three categories based on the risks associated with the device and the level of control necessary to provide reasonable assurance of safety and effectiveness. Class I devices are deemed to be low risk and are subject only to the general regulatory controls. Class II devices are moderate risk. They are subject to general controls and may also be subject to special controls. Class III devices are generally the highest risk devices. They are required to obtain pre-market approval and comply with post-market conditions of approval in addition to general regulatory controls.

Generally, companies that establish specifications for manufacture, re-label/re-package, process, and/or import devices are required to register their establishments with the FDA. They also must provide the FDA with a list of such devices and indicate the specific activities performed in their facilities.

The FDA enforces its requirements by market surveillance and periodic visits, both announced and unannounced, to inspect or re-inspect equipment, facilities, laboratories and processes to confirm regulatory compliance. These inspections may include the manufacturing facilities of subcontractors that are device manufacturers. Following an inspection, the FDA may issue a report, known as a Form 483, listing instances where the manufacturer has failed to comply with applicable regulations and/or procedures or, if observed violations are sufficiently serious, a warning letter. If the manufacturer does not adequately respond to a Form 483 or warning letter, the FDA make take enforcement action against the manufacturer or impose other sanctions or consequences, which may include:

● cease and desist orders;

● injunctions or consent decrees;

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● civil monetary penalties;

● recall, detention or seizure of products;

● operating restrictions or partial or total shutdown of production facilities;

● refusal of or delay in granting requests for clearance of 510(k) pre-market notification ("510(k)") submissions, *de novo* classification, or pre-market approval of new products or modified products;

● withdrawing 510(k) clearances, *de novo* classifications, or pre-market approvals that are already granted;

● refusal to grant export approval or export certificates for devices; and

● criminal prosecution.

The FDA also monitors promotion and, in some cases, advertising of regulated devices, including IVDs, to ensure that all promotion of the product is consistent with the device's intended use reflected in the FDA-cleared and/or -approved labeling. If the device manufacturer promotes the product in violation of advertising and promotion rules established in the FDC Act and FDA's implementing regulations, FDA may issue a so-called "Untitled Letter" or "Warning Letter" requiring the manufacturer to amend and/or remove promotion of its product that is not compliant with such rules. Additionally, FDA may take any of the enforcement actions outlined above.

#### Pre-Market Authorization and Notification
Unless subject to an exemption, medical devices require prior FDA authorization before they may be commercially marketed. Devices can be legally sold within the U.S. only if the FDA has: (i) approved a pre-market approval application prior to marketing, generally applicable to most Class III devices; (ii) cleared the device in response to a 510(k) submission, generally applicable to Class I and II devices; or (iii) reclassified the device pursuant to the *de novo* classification process, available for novel low or moderate risk devices. PMA applications, 510(k) submissions, and *de novo* classification requests require payment of substantial user fees that are increased each fiscal year.

Ova1, the first FDA-authorized blood test for the pre-operative assessment of ovarian masses, was authorized by the FDA in September 2009 under the *de novo* classification pathway. We received 510(k) clearance for Overa, our second-generation biomarker panel in March 2016.

#### 510(k) Pre-market Notification
Product marketing in the U.S. for most Class II and a limited number of Class I devices typically follows the 510(k) pathway. To obtain 510(k) clearance, a manufacturer must submit a pre-market notification demonstrating that the proposed device is substantially equivalent to a legally marketed predicate device, e.g., a device that has already received 510(k) clearance or *de novo* classification. The manufacturer must show that the proposed device has the same intended use as the predicate device, and it either has the same technological characteristics, or it is shown to be equally safe and effective and does not raise different questions of safety and effectiveness as compared to the predicate device. A 510(k) may need to be supported by clinical data.

FDA has a user fee goal to apply no more than 90 calendar review days to 510(k) submissions. During the process, FDA may issue an Additional Information request, which stops the FDA's review clock. The applicant has 180 days to respond. Therefore, to meet the user fee timeline, the total review time should not be more than 270 days, although in practice it can take longer.

After a device receives 510(k) clearance, any modification that could significantly affect its safety or effectiveness, or that would constitute a major change in its intended use, requires additional FDA authorization, typically through a new 510(k). The FDA requires each manufacturer to evaluate the impact of a device change on the existing

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510(k) clearance, but the FDA can review any such decision. If the FDA disagrees with a manufacturer's decision not to seek a new 510(k) clearance for the modified device, the agency may require the manufacturer to seek 510(k) clearance, *de novo* classification, or PMA approval. The FDA also can require the manufacturer to cease marketing and/or recall the modified device until marketing authorization is obtained.

**De Novo *Classification***

Devices of a new type that the FDA has not previously classified based on risk are automatically classified into Class III regardless of the level of risk they pose. To avoid requiring PMA review of novel low-to moderate-risk devices classified in Class III by operation of law, Congress enacted a provision that allows the FDA to classify a novel low-to moderate-risk device into Class I or II in the absence of a predicate device that would support 510(k) clearance. The FDA evaluates the safety and effectiveness of devices submitted for review under the *de novo* pathway, and devices determined to be Class II through this pathway can serve as predicate devices for future 510(k) applicants. The *de novo* pathway generally requires clinical data. As part of the *de novo* process FDA will establish special controls to help ensure the safety and effectiveness of the device.

FDA has a user fee goal to review a *de novo* request in 150 calendar review days. During the process, FDA may issue an Additional Information request, which stops the FDA's review clock. The applicant has 180 days to respond. Therefore, to meet the user fee timeline, the total review time should not exceed 330 days, although in practice it can take longer.

#### PMA Approval
A Class III product not eligible for *de novo* classification must follow the PMA approval pathway.

Results from clinical trials are required for each indication for which FDA approval is sought. After completion of the required clinical testing, a PMA application including the results of all non-clinical, clinical, and other testing and information relating to the product's marketing history, design, labeling, manufacture, and controls, is prepared and submitted to the FDA.

The PMA approval process is generally more expensive, rigorous, lengthy, and uncertain than the 510(k) pre-market notification process and *de novo* classification process and requires proof of the safety and effectiveness of the device to the FDA's satisfaction. As part of the PMA review, the FDA will typically inspect the manufacturer's facilities for compliance with the Quality Management System Regulation ("QMSR"), which specifies the elements that must be included in a quality management system, including processes for control of records, labeling, and packaging and for reporting adverse events and conducting recalls. FDA has a user fee goal to review a PMA application in 180 calendar review days, if the submission does not require advisory committee input, or 320 review days if the submission does require advisory committee input. During the process, FDA may issue a major deficiency letter, which stops the FDA's review clock. The applicant has up to 180 days to respond. Therefore, to meet the user fee timeline, the total review time should not exceed 360 days, if the submission does not require advisory committee input, and should not exceed 500 days if the submission does require advisory committee input, although in practice it could take longer.

If the FDA's evaluation of the PMA application is favorable, the FDA will issue a PMA for the approved indications, which can be more limited than those originally sought by the manufacturer. The PMA can include post-approval conditions that the FDA believes necessary to ensure the safety and effectiveness of the device including, among other things, post-approval studies and restrictions on labeling, promotion, sale and distribution. Failure to comply with the conditions of approval can result in material adverse enforcement action, including the loss or withdrawal of the approval and/or placement of restrictions on the sale of the device until the conditions are satisfied.

Even after approval of a PMA, a new PMA or PMA supplement may be required in the event of a modification to the device, its labeling or its manufacturing process. Supplements to a PMA often require the submission of the same type of information required for an original PMA, except that the supplement is generally limited to that information needed to support the proposed change from the product covered by the original PMA.

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#### Clinical Trials
Generally, data from at least one clinical trial is required to support a PMA application. Evidence from clinical studies also typically is included in a request for *de novo* classification and, less frequently, in a 510(k) submission. Clinical trials may also be conducted or continued to satisfy post-approval requirements for devices with PMAs. For significant risk investigational device studies, the FDA regulations require that human clinical investigations conducted in the U.S. be approved under an investigational device exemption ("IDE"), which must become effective before clinical testing may commence. A nonsignificant risk investigational device study does not require FDA approval of an IDE, although it does need to comply with some elements of the IDE regulations. Some studies of IVDs are entirely exempt from the IDE requirements. In some cases, one or more smaller IDE studies may precede a pivotal clinical trial intended to demonstrate the safety and efficacy of the investigational device. A 30-day waiting period after the submission of each IDE is required prior to the commencement of clinical testing in humans. If the FDA disapproves the IDE within this 30-day period, the clinical trial proposed in the IDE may not begin. Clinical trials of IVDs that meet certain regulatory criteria are exempt from the IDE regulations.

An IDE application must be supported by appropriate data, such as animal and laboratory test results, showing that it is safe to test the device in humans and that the testing protocol is scientifically sound. The IDE application must also include a description of product manufacturing and controls, and a proposed clinical trial protocol. The FDA typically grants IDE approval for a specified number of patients to be treated at specified study centers. During the study, the sponsor must comply with the FDA's IDE requirements for investigator selection, trial monitoring, reporting, and record keeping. The investigators must obtain patient informed consent, follow the investigational plan and study protocol, control the disposition of investigational devices, and comply with reporting and record keeping requirements. Prior to granting PMA approval, the FDA typically inspects the records relating to the conduct of the study and the clinical data supporting the PMA application for compliance with IDE requirements.

Clinical trials must be conducted: (i) in compliance with federal regulations; (ii) in compliance with good clinical practice ("GCP"), an international standard intended to protect the rights and health of patients and to define the roles of clinical trial sponsors, investigators, and monitors; and (iii) under protocols detailing the objectives of the trial, the parameters to be used in monitoring safety, and the effectiveness criteria to be evaluated. Clinical trials are typically conducted at geographically diverse clinical trial sites and are designed to permit the FDA to evaluate the overall benefit-risk relationship of the device and to provide adequate information for the labeling of the device when considering whether a device satisfies the statutory standard for commercialization. Clinical trials, for both significant and nonsignificant risk device studies, as well as exempt IVD studies, must be approved by an institutional review board ("IRB"), an appropriately constituted group that has been formally designated to review and monitor biomedical research involving human subjects and which has the authority to approve, require modifications in, or disapprove research to protect the rights, safety, and welfare of the human research subject. Informed consent of patients participating in the study generally must be obtained before they may participate in the study.

The FDA may order the temporary, or permanent, discontinuation of a clinical trial at any time, or impose other sanctions, if it believes that the clinical trial either is not being conducted in accordance with the FDA requirements or presents an unacceptable risk to the clinical trial patients. An IRB may also require the clinical trial it has approved to be halted, either temporarily or permanently, for failure to comply with the IRB's requirements, or may impose other conditions or sanctions.

Although the QMSR does not fully apply to investigational devices, the requirement for controls on design and development does apply to devices subject to FDA's IDE regulations. The sponsor also must manufacture the investigational device in conformity with the quality controls described in the IDE application and any conditions of IDE approval that the FDA may impose with respect to manufacturing.

#### Post-Market Requirements
After a device is placed on the market, numerous general regulatory controls apply. These include: the QMSR (which requires manufacturers to have a quality policy and procedures to ensure that devices are manufactured and records maintained in a prescribed manner with respect to manufacturing, testing, complaint handling, and record keeping),

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labeling regulations, the medical device reporting regulations (which require that manufacturers report to the FDA if their device may have caused or contributed to a death or serious injury or malfunctioned in a way that would likely cause or contribute to a death or serious injury if it were to recur), and reports of corrections and removals regulations (which require manufacturers to report recalls or removals and field corrections to the FDA if initiated to reduce a risk to health posed by the device or to remedy a violation of the FDC Act if that violation may present a risk to health). Failure to properly identify reportable events or to file timely reports, as well as failure to comply with other regulatory requirements, can subject a manufacturer to warning letters, recalls, or other sanctions and penalties.

As a manufacturer of IVDs, we are subject to regulatory oversight by the FDA under provisions of the FDC Act and regulations thereunder. We are required to register and list our IVD products with the FDA and to comply with the applicable provisions of the QMSR. We are required to submit a medical device report ("MDR") whenever we receive information that reasonably suggests that one of our devices may have caused or contributed to a death or serious injury, or where a malfunction has occurred that would be likely to cause or contribute to a death or serious injury if the malfunction were to recur. As of the date of the filing of this Annual Report on Form 10-K, we have had zero complaints that required us to submit an MDR to FDA. Additionally, we are subject to inspection by the FDA. Further, we are required to comply with FDA requirements for labeling and promotion.

Marketing and promotional activities for devices, and advertising of some restricted medical devices, are also subject to FDA oversight and must comply with the statutory standards of the FDC Act, and the FDA's regulatory requirements. The FDA's oversight of marketing and promotional activities encompasses, but is not limited to, direct-to-consumer advertising, healthcare provider-directed advertising and promotion, sales representative communications to healthcare professionals and promotional activities involving electronic media. The FDA also regulates industry-sponsored scientific and educational activities that make representations regarding product safety or efficacy in a promotional context.

Manufacturers of medical devices are permitted to promote products solely for the uses and indications that are consistent with those set forth in the approved or cleared product labeling. A number of enforcement actions have been taken against manufacturers that promote products for "off-label" uses (i.e., uses that are not described in the approved or cleared labeling), including actions alleging that claims submitted to government healthcare programs for reimbursement of products that were promoted for "off-label" uses are in violation of the Federal False Claims Act or other federal and state statutes and that the submission of those claims was caused by off-label promotion. The failure to comply with prohibitions on "off-label" promotion can result in significant monetary penalties, suspension of sales of certain products, product recalls, civil or criminal sanctions, exclusion from participating in federal healthcare programs, or other enforcement actions. Such wrongful conduct could also result in a corporate integrity agreement with the U.S. government that imposes significant administrative obligations and costs.

Violations of the FDC Act relating to the inappropriate promotion of approved products may lead to investigations alleging violations of federal and state healthcare fraud and abuse and other laws, as well as state consumer protection laws, which could, in turn, lead to additional enforcement actions, including, but not limited to, injunctions, civil monetary penalties, and/or criminal penalties.

The FDA also may impose post-market conditions of approval, such as testing, surveillance, or other measures to monitor the effects of an approved or cleared product. This is most common for devices approved under a PMA. The FDA may place conditions on a PMA-approved device that could restrict the distribution or use of the product. In addition, quality-control, manufacture, packaging, and labeling procedures must continue to conform to the QMSR after approval and clearance, and manufacturers are subject to periodic inspections by the FDA. Accordingly, manufacturers must continue to expend time, money, and effort in the areas of production and quality control to maintain compliance with the QMSR. The FDA may withdraw product approvals or recommend or require product recalls if a company fails to comply with regulatory requirements.

Clinical studies to support FDA marketing authorization of new IVD products or new indications for already-authorized IVD products must be conducted in accordance with the applicable FDA regulations.

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We also may be required to conduct post-market surveillance of medical devices as a condition of granting marketing authorization. With respect to Ova1, the FDA required us to perform post-market surveillance to gather additional data regarding test performance. This study has been completed.

#### Clinical Laboratory Improvement Amendments of 1988
Clinical laboratories operating in or testing specimens from the U.S. are subject to CLIA, and related federal and state regulations, which provide for regulation of laboratory testing. Any clinical laboratory customers using our IVDs for clinical testing in the United States will be regulated under CLIA, which establishes quality standards for all laboratory testing to ensure the accuracy, reliability and timeliness of patient test results regardless of where the test was performed. In particular, these regulations mandate that clinical laboratories must be certified by the federal government or a federally approved accreditation agency or must be located in a state that has been deemed exempt from CLIA requirements because the state has in effect laws that provide for requirements equal to or more stringent than CLIA requirements. Moreover, CLIA laboratories must meet quality assurance, quality control and personnel standards, and they must undergo proficiency testing and inspections. The CLIA standards applicable to clinical laboratories are based on test complexity, i.e., the difficulty of performing the methodology and risk of harm if results are incorrect; complexity can be "waived," "moderate complexity" or "high complexity." The FDA assigns the test complexity for commercially marketed IVDs.

Our clinical laboratory activities are subject to CLIA and related state clinical laboratory laws. In June 2014, we launched a clinical laboratory, Aspira Labs. Aspira Labs holds a CLIA Certificate of Accreditation and a CAP Certificate of Accreditation, as well as a state laboratory license or permit in California, Maryland, New York, Pennsylvania, and Rhode Island. We are subject to periodic surveys and inspections to maintain our CLIA certification, and such certification is also required to obtain payment from Medicare, Medicaid and certain other third-party payers.

#### Laboratory Developed Tests
The FDA considers LDTs to be tests that are designed, manufactured and used within a single CLIA high complexity laboratory. LDTs are performed using a variety of laboratory instruments and reagents and may also incorporate FDA-authorized IVDs that the laboratory modifies in some way and validates for its new use. The FDA historically took the position that it has the authority to regulate LDTs as medical devices under the FDC Act, but it generally exercised enforcement discretion with regard to LDTs, meaning that even though the FDA believed it could impose regulatory requirements on LDTs, including in some cases requirements to obtain pre-market approval or clearance of LDTs, it generally chose not to enforce those requirements.

On May 6, 2024, the FDA published a final rule amending the definition of an IVD device to include IVDs manufactured by a clinical laboratory, effectively codifying its position that LDTs are IVDs and, therefore, that LDTs fall under FDA's regulatory authority. The final rule also announced the FDA's intention to phase out its general enforcement discretion policy. FDA intended to phase in the new requirements beginning on May 6, 2025.

On September 19, 2025, the FDA, at the direction of a federal district court, formally rescinded its May 2024 final rule regulating LDTs as medical devices. In a March 31, 2025 ruling, the U.S. District Court for the Eastern District of Texas found that LDTs do not meet the definition of a medical device under the FDC Act and the FDA therefore lacks jurisdiction to regulate them. The FDA has not indicated how it will interpret the court ruling or whether it will seek a different regulatory approach with respect to LDTs or components thereof.

Over the years, various legislative proposals addressing the FDA's oversight of LDTs have been introduced in Congress. In June 2021, Congress introduced the Verifying Accurate, Leading-edge IVCT Development Act ("VALID Act") to establish a new risk-based regulatory framework for in vitro clinical tests ("IVCTs"), including IVDs, LDTs, collection devices and instruments used with such tests. This legislation was re-introduced in 2023 but was not enacted. The VALID Act was again re-introduced in 2025, indicating that there remains debate about whether and how LDTs should be regulated in the U.S.

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#### Clinical Decision Support Software
The FDA has become increasingly active in addressing the regulation of software used to support clinical decision making. In 2016, the 21st Century Cures Act (the "Cures Act"), among other things, amended the medical device definition in the FDC Act to exclude certain software from FDA regulation, including clinical decision support ("CDS software"), that meets certain criteria. CDS software is exempt from the medical device definition if it: (a) displays, analyzes or prints medical information about a patient or other medical information; (b) is intended for the purpose of supporting or providing recommendations about a patient's care to a healthcare professional ("HCP") user; and (c) provides sufficient information about the basis for the recommendations to the HCP user, so that the HCP user does not rely primarily on any of the recommendations to make a clinical decision about an individual patient; unless (d) the software function acquires, processes, or analyzes a medical image, a signal from an in vitro diagnostic device, or a pattern or signal from a signal acquisition system.

On September 28, 2022, the FDA issued a final guidance document interpreting the Cures Act as it pertains to CDS software. FDA updated the guidance in 2026 to provide further clarification regarding the agency's interpretation of the CDS criteria, including in particular the types of inputs that would be considered a "signal" or "pattern", and the types outputs that would be considered to provide a diagnosis, and thereby excluded from the CDS exemption.

#### Foreign Government Regulation of Our Products
Medical device laws and regulations are in effect in many of the countries in which we may do business outside the United States. These range from comprehensive device approval requirements for some or all of our potential future medical device products, to requests for product data or certifications. The number and scope of these requirements are increasing. In addition, products which have not yet been cleared or approved for domestic commercial distribution may be subject to the FDA Export Reform and Enhancement Act of 1996. Each country also maintains its own regulatory review process, tariff regulations, duties and tax requirements, product standards, and labeling requirements. In February 2015, Aspira also received ISO 13485 (currently ISO13485:2016) certification for our quality management system from the British Standards Institution (BSI), one of the world's leading certification bodies. In March 2015, Ova1 was CE marked, a requirement for marketing the test in the European Union. In October 2015, we announced registration of the CE mark for and clearance to market Overa in the European Union.

#### Privacy and Security of Health Information
The federal Health Insurance Portability and Accountability Act of 1996, or HIPAA, as amended by the Health Information Technology for Economic and Clinical Health Act, or HITECH, and final omnibus rules, were issued by HHS to protect the privacy and security of protected health information used or disclosed by health care providers, such as us. HIPAA also regulates standardization of data content, codes and formats used in health care transactions and standardization of identifiers for health plans and providers. Penalties for violations of HIPAA regulations include civil and criminal penalties. In addition to federal privacy regulations, a number of state and international laws govern confidentiality of health information.

#### Health Care Fraud and Abuse
The federal Anti-Kickback Statute makes it a felony for a provider or supplier, including a laboratory, to knowingly and willfully offer, pay, solicit or receive remuneration, directly or indirectly, in order to induce business that is reimbursable under any federal health care program, including Medicare and Medicaid. A violation of the federal Anti-Kickback Statute may result in imprisonment for up to five years and/or criminal fines of up to $250,000 for an individual. Companies may be criminally fined up to $500,000 and may also be subject to civil assessments and exclusion from participation in Medicare, Medicaid, and other federal health care programs.

Actions that violate the federal Anti-Kickback Statute may also be subject to liability under the Federal False Claims Act, which prohibits knowingly presenting or causing to be presented a false or fraudulent claim for payment to the U.S. Government. Although the federal Anti-Kickback Statute applies only to federal health care programs, a number

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of states have enacted statutes substantially similar to the federal Anti-Kickback Statute pursuant to which similar types of prohibitions are made applicable to all other health plans and third-party payors.

The Eliminating Kickbacks in Recovery Act ("EKRA") makes it a federal crime to knowingly and willfully solicit or receive any remuneration, directly or indirectly, in return for referring a patient or patronage any laboratory. EKRA also prohibits paying or offering any remuneration directly or indirectly: (A) to induce a referral of an individual to a laboratory; or (B) in exchange for an individual using the services of a laboratory. Although EKRA's language is similar to the language of the federal Anti-Kickback Statute, EKRA is broader than the AKS in that it applies to all health care benefit programs, including private payors, while the AKS applies only to items and services paid for by federal health care programs.

Federal and state law enforcement authorities scrutinize arrangements between laboratories and potential referral sources to ensure that the arrangements are not designed as a mechanism to induce patient care referrals. The law enforcement authorities and the courts have also demonstrated a willingness to look behind the formalities of a transaction to determine the underlying purpose of payments between health care providers and actual or potential referral sources. Generally, courts have taken a broad interpretation of the scope of the federal Anti-Kickback Statute, holding that the statute may be violated if merely one purpose of a payment arrangement is to induce referrals, even if the arrangement has other, legitimate purposes.

In December 1994, the HHS Office of Inspector General, or OIG, issued a Special Fraud Alert on arrangements for the provision of clinical laboratory services. The Fraud Alert set forth a number of practices allegedly engaged in by some clinical laboratories and health care providers that raise issues under the federal fraud and abuse laws, including the federal Anti-Kickback Statute. The OIG emphasized in the Special Fraud Alert that when one purpose of such arrangements is to induce referrals of program-reimbursed laboratory testing, both the clinical laboratory and the health care provider (e.g., physician) may be liable under the federal Anti-Kickback Statute and may be subject to criminal prosecution and exclusion from participation in the Medicare and Medicaid programs.

Recognizing that the federal Anti-Kickback Statute is broad and may technically prohibit many innocuous or beneficial arrangements within the health care industry, Congress authorized, and HHS has issued, a series of regulatory "safe harbors." These safe harbor regulations set forth certain provisions which, if all of their requirements are met, will assure health care providers and other parties that they may not be prosecuted under the federal Anti-Kickback Statute. Although full compliance with these provisions ensures against prosecution under the federal Anti-Kickback Statute, the failure of a transaction or arrangement to fit within a specific safe harbor does not necessarily mean that the transaction or arrangement is illegal or that prosecution under the federal Anti-Kickback Statute will be pursued. A non-safe harbored arrangement is evaluated by government enforcement agencies on a case-by-case basis.

In addition, the federal False Claims Act prohibits a person from knowingly submitting or causing to be submitted a false claim or making a false record or statement material to a false claim in order to secure payment by the federal government. Violation of the federal False Claims Act may result in fines of up to three times the actual damages sustained by the government, plus mandatory civil penalties of up to $27,894 for each separate false claim.

Moreover, a federal law directed at "self-referrals," commonly known as the Stark Law, prohibits, with certain exceptions, laboratories from presenting or causing to be presented claims to Medicare and Medicaid for laboratory tests referred by physicians who personally, or through a family member, have an investment interest in, or a compensation arrangement with, the clinical laboratory performing the tests. A person who engages in a scheme to circumvent the Stark Law's referral prohibition may be fined up to $100,000 for each such arrangement or scheme. In addition, any person who presents or causes to be presented a claim to the Medicare or Medicaid program in violation of the Stark Law is subject to civil monetary penalties of up to $15,000 per claim submission, an assessment of up to three times the amount claimed, and possible exclusion from participation in federal health care programs. Claims submitted in violation of the Stark Law may not be paid by Medicare or Medicaid, and any person collecting any amounts with respect to any such prohibited claim is obligated to refund such amounts. Many states, including California, also have "anti-self-referral" and other laws that are not limited to Medicare and Medicaid referrals.

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Further, in addition to the privacy and security regulations described above, HIPAA created two federal crimes: health care fraud and false statements relating to health care matters. The health care fraud statute prohibits knowingly and willfully executing a scheme to defraud any health care benefit program, including both government and private payors. A violation of this statute is a felony and may result in fines, imprisonment and/or exclusion from government sponsored programs. The false statements statute prohibits knowingly and willfully falsifying, concealing or covering up a material fact or making any materially false, fictitious or fraudulent statement in connection with the delivery of or payment for health care benefits, items or services. A violation of this statute is a felony and may result in fines and/or imprisonment.

Finally, federal law prohibits any entity from offering or transferring to a Medicare or Medicaid beneficiary any remuneration that the entity knows or should know is likely to influence the beneficiary's selection of a particular provider, practitioner or supplier of Medicare or Medicaid payable items or services, including waivers of copayments and deductible amounts (or any part thereof) and transfers of items or services for free or for other than fair market value. Entities found in violation may be liable for civil monetary penalties of up to $20,000 for each wrongful act.

#### Employees
As of December 31, 2025, we had 36 full-time employees and two part-time employees. We generally engage independent contractors on a part-time basis from time to time.

#### Corporate Information
We were originally incorporated in 1993, and we had our initial public offering in 2000. Our executive offices are located at 12117 Bee Caves Road, Building III, Suite 100, Austin, Texas 78738, and our telephone number is (512) 519-0400. We maintain a website at www.aspirawh.com where general information about us is available.

#### Information About Us
We file annual reports, quarterly reports, current reports, proxy statements, and other information with the SEC.

The SEC maintains an Internet website, www.sec.gov, that contains reports, proxy statements, and other information regarding issuers that file electronically with the SEC.

The information contained on our websites is not incorporated by reference in this Annual Report on Form 10-K, and should not be considered a part of this Annual Report on Form 10-K.

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| **ITEM 1A.** | **RISK FACTORS** |

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*Investing in our securities involves a high degree of risk. You should carefully consider the following risk factors and uncertainties together with all of the other information contained in this Annual Report on Form 10-K, including our audited consolidated financial statements and the accompanying notes in Part II Item 8, "Consolidated Financial Statements and Supplementary Data." If any of the following risks materializes, our business, financial condition, results of operations and growth prospects could be materially adversely affected, and the value of an investment in our common stock may decline significantly. The risks and uncertainties described below are not the only ones we face. Additional risks and uncertainties not presently known to us or that we currently deem immaterial may also materially adversely affect our business, financial condition, results of operations and growth prospects.*

#### RISKS RELATED TO OUR BUSINESS AND INDUSTRY

#### If we are unable to increase the volume of OvaSuite sales, our business, results of operations and financial condition will be adversely affected.
We have experienced significant operating losses each year since our inception, and we expect to incur a net loss for fiscal year 2026. Our losses have resulted principally from costs incurred in cost of revenue, sales and marketing, general and administrative costs and research and development. The number of tests performed in 2025 and in 2024 was

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22,515 and 24,305, respectively. If we are unable to substantially increase the volume of OvaSuite sales, our business, results of operations and financial condition will be adversely affected.

***There is substantial doubt about our ability to continue as a going concern, and this may adversely affect our stock price and our ability to raise capital.***

We have incurred significant losses and negative cash flows from operations since inception and have an accumulated deficit of $544,177,000 as of December 31, 2025. We also expect to incur a net loss and negative cash flows from operations in 2026 and have limited cash balances. Given these conditions, there is substantial doubt about our ability to continue as a going concern. The substantial doubt about our ability to continue as a going concern may adversely affect our stock price and our ability to raise capital. Our independent registered public accounting firm has also included in its report an explanatory paragraph regarding this uncertainty.

We believe that successful achievement of our business objectives will require additional financing. We expect to raise capital through a variety of sources that may include public or private equity offerings, debt financing, collaborations, licensing arrangements, grants and government funding and strategic alliances. However, in part due to our low stock price, additional financing may not be available when needed or on terms acceptable to us. If we are unable to obtain additional capital, we may not be able to continue sales and marketing, research and development, distribution or other operations on the scope or scale of current activity and that could have a material adverse effect on our business, results of operations and financial condition. The accompanying financial statements do not include any adjustments that may be necessary should we be unable to continue as a going concern.

***We may need to sell additional shares of our common stock or other securities in the future to meet our capital requirements, which could cause significant dilution.***

Until such time, if ever, as we can generate substantial product revenues, we expect to finance our cash needs through a combination of the issuance of common stock in public or private equity offerings, debt financings, exercise of common stock warrants, collaborations, licensing arrangements, grants and government funding and strategic alliances. Our management believes the successful achievement of our business objectives will require additional financing through one or more of these avenues. To the extent that we raise additional capital through the sale of equity or convertible debt, such financing may be dilutive to stockholders. Debt financing, if available, may involve restrictive covenants and potential dilution to stockholders. Furthermore, a perception that future sales of our common stock in the public market are likely to occur could affect prevailing trading prices of our common stock.

As of December 31, 2025, we had 43,480,411 shares of our common stock outstanding and 790,376 shares of our common stock reserved for future issuance to employees, directors and consultants pursuant to our employee stock plans, which excludes 3,114,229 shares of our common stock that were subject to outstanding options. In addition, as of December 31, 2025, warrants to purchase 21,235,745 shares of our common stock were outstanding. These warrants are exercisable at the election of the holders thereof, in accordance with the terms of the related warrant, at an average exercise price of $0.98 per share.

The exercise of all or a portion of our outstanding options and warrants will dilute the ownership interests of our stockholders.

***We will need to raise additional capital in the future, and if we are unable to secure adequate funds on terms acceptable to us, we may be unable to execute our business plan.***

We will seek to raise additional capital through the issuance of equity or debt securities in the public or private markets, or through a collaborative arrangement or sale of assets. Additional financing opportunities may not be available to us, or if available, may not be on favorable terms. The availability of financing opportunities will depend, in part, on market conditions, and the outlook for our business. Any future issuance of equity securities or securities convertible into equity could result in substantial dilution to our stockholders, and the securities issued in such a financing may have rights, preferences or privileges senior to those of our common stock. If we are unable to obtain additional capital, we may not

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be able to continue our sales and marketing, research and development, distribution or other operations on the scope or scale of our current activity.

***Failures by third-party payers to reimburse for our products and services or changes in reimbursement rates could materially and adversely affect our business, financial condition and results of operations. In addition, changes in medical society guidelines may also adversely affect payers and result in a material change in coverage, adversely affecting our business, financial condition and results of operations.***

The great majority of laboratory tests in the United States are paid for by third party payers. Accordingly, our current revenues are from, and our future revenues will be dependent upon, third-party reimbursement payments to Aspira Labs. Insurance coverage and reimbursement rates for diagnostic tests are uncertain, subject to change and particularly volatile during the early stages of commercialization. There remain questions as to what extent third-party payers, like Medicare, Medicaid and private insurance companies will provide coverage for our products and for which indications. Some payers have determined not to cover our tests. While Novitas Solutions, the Medicare Administrative Contractor responsible for paying Medicare claims for all Aspira laboratory tests, has determined to cover Ova1, there is no assurance that they will continue to do so. Moreover, while The CMS has issued PAMA reimbursement rates for Ova1 effective January 1, 2018, there is no guarantee that the payment rates will not be reduced. Although the PAMA legislation allows for no more than a 15% fee reduction between 2025 and 2026, uncertainty regarding reimbursement rates could create payment uncertainty from other payers as well. The reimbursement rates for Ova1 and OvaWatch are reviewed by third-party payers. We have experienced volatility in the coverage and reimbursement of our products due to contract negotiation with third-party payers and implementation requirements, and the reimbursement amounts we have received from third-party payers varies from payer to payer, and, in some cases, the variance could be material.

Third-party payers, including private insurance companies as well as government payers such as Medicare and Medicaid, have increased their efforts to control the cost, utilization and delivery of healthcare services including increased use of Laboratory Benefits Management firms, who create policy and implement utilization management strategies for their payer clients to ensure tests are medically necessary. In addition, more payers are implementing pre-authorization requirements for our testing. These measures have resulted in reduced payment rates and decreased utilization of our tests. Further, the trend among many payers is to limit the size of their lab networks, which is making it more difficult to secure preferred provider contracts for some services. From time to time, Congress has considered and implemented changes to the Medicare fee schedules in conjunction with budgetary legislation, and pricing for tests covered by Medicare is subject to change at any time, although PAMA has established specific dates by which they will make any changes. Even if favorable coverage and reimbursement status is attained for one or more products by governmental and commercial third-party payers, less favorable coverage policies and reimbursement rates may be implemented in the future. Reductions in third-party payer reimbursement rates may occur in the future. Reductions in the price at which our products are reimbursed could have a material adverse effect on our business, results of operations and financial condition. If we are unable to establish and maintain broad coverage and adequate reimbursement for our products or if third-party payers change their coverage or reimbursement policies with respect to our products, our business, financial condition and results of operations could be materially adversely affected.

***Failure to continue coverage of Ova1 through Novitas, our Medicare Administrative Contractor for Ova1, could materially and adversely affect our business, financial condition and results of operation.***

Since 2013, Ova1 has been listed as a covered service in the Biomarkers for Oncology Local Coverage Determination (the "Biomarkers for Oncology LCD") issued by Novitas, the Medicare Administrative Contractor responsible for payment of Medicare claims for all Aspira Labs tests. In June 2023, in conjunction with the publication of a final "Genetic Testing for Oncology" LCD (the "Genetic Testing LCD"), Novitas announced that it intended to retire the Biomarkers for Oncology LCD effective July 17, 2023, and that at that time, non-genetic tests currently identified as covered in that LCDs (like Ova1) would be considered for payment based on Medicare medically reasonable and necessary threshold for coverage.

On July 6, 2023, Novitas issued a statement announcing that the Genetic Testing LCD would not go into effect on July 17, 2023 as planned, and that a new proposed LCD would be published for public comment. Novitas issued a replacement proposed LCD for public comment on July 27, 2023. The Biomarkers for Oncology LCD remains in effect.

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All OvaSuite tests (Ova1, Overa, Ova1Plus and OvaWatch) are protein-based multivariate index assays and were not impacted by the now-withdrawn Genetic Testing LCD. While we do not believe Novitas intends to eliminate Ova1 coverage, it is impossible to assess the likelihood or potential impact, if any, of future actions to be taken by Novitas with respect to the release of a replacement Genetics Testing LCD, or a change to the content or status of the Biomarkers for Oncology LCD, on the coverage and related revenue of Ova1, and such impact may be material to our business, results of operations and financial condition. We are monitoring developments closely and believe additional due process would be required if the activities contemplated by Novitas change the coverage determination for Ova1.

#### Failure to expand commercial, Medicare or Medicaid coverage for our products could materially and adversely affect our business, financial condition and results of operations.
We have implemented strategies to expand payer coverage for our ovarian cancer risk assessments, including securing coverage for OvaWatch that is consistent with existing coverage for Ova1. In November 2023, CMS approved our request to provide reimbursement for OvaWatch that is consistent with the reimbursement for Ova1 at $897 per test. However, there can be no assurances that we will be able to secure additional payer coverage for Ova1 and comparable coverage for OvaWatch, or that that the reimbursement rate for OvaWatch will not be reduced. Failure to expand payer coverage and maintain adequate reimbursement rates may have a significant negative impact on product adoption and our results of operations.

***We may not succeed in improving existing or developing additional diagnostic products, and, even if we do succeed in developing additional diagnostic products, the diagnostic products may never achieve significant commercial market acceptance.***

Our technologies are new and complex and are subject to change as new discoveries are made. New discoveries and advancements in the diagnostic field are essential if we are to foster the adoption of our product offerings. Development of our existing technologies remains a substantial risk to us due to various factors, including the scientific challenges involved within our laboratory, as well as products that are offered in a decentralized platform such as Aspira Synergy, our ability to find and collaborate successfully with others working in the diagnostic field, our ability to obtain sufficient samples to complete the design and development of our algorithms and competing technologies, which may prove more successful than our technologies, as well as failure to complete analytical and clinical validation studies and failure to demonstrate sufficient clinical utility to continue to build positive medical policy among payers.

Our failure to achieve the intended development outcome either ourselves or through a collaboration may result in an impact to our commercial success of our risk assessment screens for endometriosis or other product launches.

Our success depends on our ability to continue to develop and commercialize diagnostic products. There is considerable risk in developing diagnostic products based on our biomarker discovery efforts, as candidate biomarkers may fail to demonstrate clinical validity in larger clinical studies or may not achieve acceptable levels of analytical accuracy. For example, markers being evaluated for one or more next-generation diagnostic tests may not be validated in downstream pre-clinical or clinical studies, once we undertake and perform such studies. In addition, development of products combining biomarkers with imaging, patient risk factors or other risk indicators carry higher than average risks due to technical, clinical and regulatory uncertainties. While we have a published proof of concept on combining Ova1 and imaging, for example, our ability to develop, verify and validate an algorithm that generalizes to routine testing populations cannot be guaranteed. In addition, our efforts to develop other diagnostic tests, such as ENDOinform and OVAinform, are in the development phase, and future pre-clinical or clinical studies may not support our early data. If successful, the regulatory pathway and clearance/approval process may require extensive discussion with applicable authorities and possibly advisory panels. These pose considerable risk in projecting launch dates, requirements for clinical evidence and eventual pricing and return on investment. Although we are engaging important stakeholders representing gynecologic oncology, benign gynecology, patient advocacy, women's health research, legislators, payers, and others, success, timelines and value will be uncertain and require active management at all stages of innovation and development.

Clinical testing is expensive, can take many years to complete and can have an uncertain outcome. Clinical failure can occur at any stage of the testing. Clinical trials for our next generation ovarian cancer tests, and other future diagnostic tests, may produce negative or inconclusive results, and we may decide, or regulators may require us, to conduct additional

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clinical and/or non-clinical testing on these tests. In addition, the results of our clinical trials may identify unexpected risks relative to safety or efficacy, which could complicate, delay or halt clinical trials, or result in the denial of regulatory approval by the FDA and other regulatory authorities.

If we do succeed in developing additional diagnostic tests with acceptable performance characteristics, we may not succeed in achieving commercial market acceptance for those tests. Our ability to successfully commercialize our OvaSuite products and Aspira Synergy platform will depend on many factors, including:

● our ability to drive adoption of our products;

● our success in establishing new clinical practices or changing previous ones;

● our ability to develop business relationships with diagnostic or laboratory companies that can assist in the commercialization of these products in the U.S. and globally; and

● the scope and extent of the agreement by Medicare and third-party payers to provide full or partial reimbursement coverage for our products, which may impact patients' willingness to pay for our products and may influence physicians' decisions to recommend or use our products.

These factors present obstacles to commercial acceptance of our existing and potential diagnostic products, for which we will have to spend substantial time and financial resources to overcome, and there is no guarantee that we will be successful in doing so. Our inability to do so successfully would prevent us from generating revenue from OvaSuite and developing future diagnostic products.

In October 2022, we announced the launch of a comarketing arrangement for the Ova1Plus workflow with BioReference. Under terms of the agreement, the Aspira and BioReference sales teams collaborate to sell Ova1Plus to gynecologists and other women's healthcare providers nationwide. In November 2024, Aspira and BioReference announced the expansion of the sale team collaboration to include OvaWatch. If we are unable to collaborate successfully, it may affect our ability to improve adoption of our Ova1Plus test or to successfully secure additional commercial collaborations.

#### The diagnostics market is competitive, and we may not be able to compete successfully, which would adversely impact our ability to generate revenue.
Our principal competition currently comes from the many clinical options available to medical personnel involved in clinical decision making. For example, rather than ordering an OvaSuite test for a woman with an adnexal mass, obstetricians, gynecologists and gynecologic oncologists may choose a different clinical option or none at all. If we are not able to convince clinicians that these products provide significant improvement over current clinical practices or to change their ordering habits, our ability to commercialize OvaSuite products will be adversely affected.

Competitive offerings include Fujirebio Diagnostics' FDA cleared ROMA test. ROMA combines two tumor markers and menopausal status into a numerical score using a publicly available algorithm. ROMA is a competitive test with the Ova1Plus workflow that has adversely impacted and may continue to materially adversely impact our revenue. In addition, competitors, AOA Dx, ClearNote, Cleo Diagnostics, Mercy BioAnalytics, and others have publicly disclosed that they have been or are currently working on ovarian cancer diagnostic assays. Exact Sciences, Grail, and others are working on MCED tests that include ovarian cancer detection. Academic institutions periodically report new findings in ovarian cancer diagnostics that may have commercial value.

A number of diagnostic and academic organizations have announced plans or published studies related to the development of a non-invasive diagnostic tool for the identification of endometriosis. Competitors for our endometriosis offerings include, but are not limited to, Afynia, DotLab, Endodiag, HERA Biotech, Heranova, Proteomics International. and Ziwig. Our failure to compete with any competitive diagnostic assay if and when commercialized could adversely affect our business, financial condition and results of operations.

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We have priced our products at a point that recognizes the value-added by their increased sensitivity for detecting ovarian malignancy. If others develop a test that is viewed to be similar to any of these products in safety and efficacy but is priced at a lower point, we or our strategic partners may have to lower the price of that product in order to effectively compete, which would impact our margins and potential for profitability.

***We are currently offering and developing multiple tests as LDTs and intend to develop and perform LDTs at Aspira Labs in the future. If FDA were to begin actively regulating our tests, we would incur substantial costs and delays associated with the effort to obtain premarket 510(k) clearance, de novo classification, or premarket approval and incur costs related to compliance with post-market controls.***

With the exception of Ova1 and Overa, we believe our tests are LDTs. The FDA generally considers an LDT to be a test that is designed, manufactured, and used within a single laboratory that is certified under CLIA and meets the regulatory requirements under CLIA to perform high complexity testing. Our laboratories are currently regulated under CLIA and accredited by the College of American Pathologists ("CAP"). We are subject to additional federal and state laws and regulations. The FDA issued a final rule in May 2024 that would have subjected many LDTs to regulatory requirements including, in some cases, premarket authorization. A federal district court vacated the FDA final rule in May 2025, holding that LDTs are not subject to FDA regulation. The FDA rescinded the final rule in September 2025. The FDA has not indicated how it will interpret the court ruling or whether it will seek a different regulatory approach with respect to LDTs or components thereof. In June 2025, Congress re-introduced the Verifying Accurate, Leading-edge IVCT Development Act ("VALID Act") to establish a new risk-based regulatory framework for in vitro clinical tests ("IVCTs"), including IVDs, LDTs, collection devices and instruments used with such tests. This legislation was previously introduced in 2021 and 2023. If we are unable to comply with requirements that the FDA , either on its own initiative or at the direction of Congress, decides to implement in the future, or if we cannot do so within the timeframes specified by the FDA, we may be forced to stop selling our tests or be required to modify claims or make such other changes while we update our processes.

If we develop tests in the future that are subject to FDA clearance, approval or *de novo* classification, our business, results of operations and financial condition will be negatively affected until such a review is completed and clearance, approval or *de novo* classification to market were obtained. There can be no assurance that any tests we develop will be cleared, approved or classified on a timely basis, if at all. Obtaining FDA clearance, approval or *de novo* classification for diagnostics can be expensive, time consuming and uncertain, and for higher-risk devices generally takes several years and requires detailed and comprehensive scientific and clinical data. Ongoing compliance with FDA regulations for those tests will increase the cost of conducting our business, significantly affect our operations, and could have a significant negative impact on our financial performance.

***Certain of our diagnostic tests and associated software are subject to ongoing regulation by the FDA, and we may develop additional FDA-regulated diagnostic tests and software in the future. Any failure to comply with applicable FDA regulations for our current FDA regulated products, and any delay by or failure of the FDA to authorize future products we submit to the FDA may adversely affect our business, results of operations and financial condition.***

Some of our currently marketed and potential future diagnostic products and associated software are, or may in the future be, subject to regulatory oversight by the FDA under provisions of the FDC Act and regulations thereunder, including regulations governing the development, marketing, labeling, promotion, manufacturing and export of our products. Failure to comply with applicable pre-market and post-market requirements can lead to sanctions, including withdrawal of products from the market, recalls, refusal to authorize government contracts, product seizures, civil money penalties, injunctions and criminal prosecution.

The FDC Act requires that medical devices introduced to the United States market, unless exempted by regulation, be authorized by FDA pursuant to either the premarket notification pathway, known as 510(k) clearance, the *de novo* classification pathway, or the PMA pathway. The FDA granted a request for a *de novo* classification for Ova1 in September 2009, and we commercially launched Ova1 in March 2010. In March 2016, we received FDA 510(k) clearance for a second-generation biomarker panel known as Ova1 Next Generation, which we call Overa. Ova1 was the first FDA-authorized blood test for the pre-operative assessment of ovarian masses. With respect to devices reviewed through the 510(k) process, we may not market a device until it is determined that our product is substantially equivalent to a legally

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marketed device known as a predicate device. A 510(k) submission may involve the presentation of a substantial volume of data, including clinical and analytical data, as well as extensive information regarding software. The FDA may agree that the product is substantially equivalent to a predicate device and allow the product to be marketed in the United States. On the other hand, the FDA may determine that the device is not substantially equivalent and require a PMA or *de novo* classification, or require further information, such as additional test data, including data from clinical studies, before it is able to make a determination regarding substantial equivalence. By requesting additional information, the FDA can delay market introduction of our products. Delays in receipt of or failure to receive any necessary 510(k) clearance, *de novo* classification, or PMA, or the imposition of stringent restrictions on the labeling and sales of our products, could have a material adverse effect on our business, results of operations and financial condition. If the FDA determines that a PMA is required for any of our potential future clinical products, the application will require extensive clinical studies, manufacturing information and could require review by an FDA advisory panel comprising experts outside the FDA. Clinical studies to support a 510(k) submission, *de novo* classification or a PMA application would need to be conducted in accordance with FDA requirements. Failure to comply with FDA requirements could result in the FDA's refusal to accept the submission or denial of the application. We cannot ensure that any necessary 510(k) clearance, *de novo* classification, or PMA will be granted on a timely basis, or at all. To the extent we seek FDA 510(k) clearance, *de novo* classification or FDA pre-market approval for other diagnostic tests, any delay by or failure of the FDA to clear, classify, or approve those diagnostic tests may adversely affect our consolidated revenues, results of operations and financial condition.

Certain changes to medical devices that a manufacturer makes after receiving a 510(k) clearance, *de novo* classification, or PMA may trigger the need for additional FDA authorization. In the case of a 510(k)-cleared device, FDA requires a new marketing authorization for significant changes or modifications made in the design, components, method of manufacture or intended use of a device, including changes or modifications to a 510(k)-cleared device that could significantly affect the device's safety or effectiveness, or would constitute a major change or modification in the device's intended use. The type of submission needed—510(k), *de novo* classification, or PMA—will depend on the specific modification the manufacturer seeks to make. FDA expects the manufacturer to make the determination of whether a new marketing application is needed by applying existing agency guidance, but FDA may independently review, and may disagree with, our decision. If we make modifications to our marketed devices, we may be required to seek additional clearances, *de novo* classifications, or PMAs which, if not granted, would prevent us from selling the modified device. If we conclude that a modification does not require submission of a new marketing application and FDA disagrees with the decision, we may be required to submit new 510(k) notifications, *de novo* classification requests, or premarket approval applications and may be required to cease marketing of or to recall the modified devices until marketing authorization is obtained and could additionally be subject to regulatory fines or penalties. Any recall or FDA requirement that we seek additional approvals or clearances could result in significant delays, fines, increased costs associated with modification of a product, loss of revenue and potential operating restrictions imposed by the FDA.

Certain of our software algorithms have been authorized for marketing by FDA as part of our cleared or *de novo* classified IVDs. If any of the software that we use in our LDTs or that we make available to third parties is determined by FDA to be non-exempt clinical decision support software, this could impede our ability to offer our tests or distribute our software to third parties and we could incur substantial costs and delays associated with trying to obtain premarket 510(k) clearance, *de novo* classification, or premarket review and incur costs associated with complying with post-market controls.

***If we or our suppliers fail to comply with FDA requirements for production, marketing and post-market monitoring of our products, we may not be able to market our products and services and may be subject to stringent penalties, product restrictions or recall.***

Failure to comply with FDA requirements for post-market monitoring of our products may affect the commercialization of our products, therefore adversely affecting our business. The FDA granted the request for *de novo* classification for Ova1 in September 2009 and cleared Overa in March 2016. Post-market surveillance studies were conducted to further analyze performance of Ova1 and Overa. These studies have been completed and closed with the FDA.

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Additionally, if the FDA were to view any of our actions as non-compliant, it could initiate enforcement actions, such as a warning letter and possible imposition of penalties. For instance, we are subject to a number of FDA requirements, including compliance with the FDA's QMSR requirements, which establish extensive requirements for quality assurance and control as well as manufacturing procedures. Failure to comply with these regulations could result in enforcement actions for us or our potential suppliers. Adverse FDA actions in any of these areas could significantly increase our expenses and reduce our revenue. We will need to undertake steps to maintain our operations in line with the FDA's QMSR requirements. Some components of Ova1 and Overa are manufactured by other companies and we are required to ensure that, to the extent that we incorporate those components into our finished Ova1 and Overa products, we use those components in compliance with QMSR. Any failure to do so would have an adverse effect on our ability to commercialize the Ova1Plus workflow. Our suppliers that manufacture finished devices at their manufacturing facilities that we use in our products and services are subject to periodic regulatory inspections by the FDA and other federal and state regulatory agencies. Our facility also is subject to FDA inspection. We or our suppliers may not satisfy such regulatory requirements, and any such failure to do so may adversely affect our business, financial condition and results of operations.

***If our suppliers fail to produce acceptable or sufficient stock, fail to supply stock due to supply shortages, make changes to the design or labeling of their biomarker kits or discontinue production of existing biomarker kits or instrument platforms, we may be unable to meet market demand for OvaSuite products.***

The commercialization of our OvaSuite tests depends on the supply of seven different immunoassay kits from third-party manufacturers that run on automated instruments. Failure by any of these manufacturers to produce kits that meet our specifications and pass our quality control measures might lead to back-order and/or loss of revenue due to missed sales and customer dissatisfaction. In addition, if the design or labeling of any kit were to change, continued OvaSuite supply could be threatened since new validation and submission to the FDA for review could be required as a condition of sale. Discontinuation of any of these kits could require identification, validation and submission to FDA of a revised OvaSuite design. Likewise, discontinuation or failure to support or service the instruments may pose risk to ongoing operations.

***Changes in healthcare policy could increase our costs and adversely impact sales of and reimbursement for our tests, which would have an adverse effect on our business, financial condition and results of operations.***

PAMA established a Medicare reimbursement system for clinical laboratories beginning in 2018 that is based on rates paid to laboratories by private payers. The CMS also issued various regulations and guidance to implement PAMA that require certain laboratories to report information on the rates private payers pay them for laboratory tests, including Multianalyte Assays with Algorithmic Analyses. In addition to these changes, a number of states are also contemplating significant reform of their healthcare reimbursement policies. We expect that there will be additional health reform initiatives by legislators at both the federal and state levels, regulators and third-party payers to reduce costs while expanding individual healthcare benefits. Certain of these changes could impose additional limitations on the rates we will be able to charge for our current and future products or the amounts of reimbursement available for our current and future products from governmental agencies or other third-party payers. We cannot predict whether future healthcare initiatives will be implemented at the federal or state level, or the effect any future legislation or regulation will have on us. Other changes to healthcare laws may adversely affect our business, financial condition and results of operations.

#### We are subject to environmental laws and potential exposure to environmental liabilities.
We are subject to various international, federal, state and local environmental laws and regulations that govern our operations, including the handling and disposal of non-hazardous and hazardous wastes, the recycling and treatment of electrical and electronic equipment, and emissions and discharges into the environment. Failure to comply with such laws and regulations could result in costs for corrective action, penalties or the imposition of other liabilities. We are also subject to laws and regulations that impose liability and clean-up responsibility for releases of hazardous substances into the environment. Under certain of these laws and regulations, a current or previous owner or operator of property may be liable for the costs to remediate hazardous substances or petroleum products on or from its property, without regard to whether the owner or operator knew of, or caused, the contamination, as well as incur liability to third parties affected by such contamination. The presence of, or failure to remediate properly, such substances could adversely affect the value and the ability to transfer or encumber such property.

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***The operation of Aspira Labs requires us to comply with numerous laws and regulations, which is expensive and time-consuming and could adversely affect our business, financial condition and results of operations, and any failure to comply could result in exposure to substantial penalties and other harm to our business.***

In June 2014, we launched a clinical laboratory, Aspira Labs, in Texas. Aspira Labs holds a CLIA Certificate of Accreditation and a CAP Certificate of Accreditation, as well as a state laboratory license or permit in California, Maryland, New York, Pennsylvania and Rhode Island. This allows the lab to perform Ova1 and Overa testing (through the Ova1Plus workflow) on a national basis. We are subject to periodic surveys and inspections to maintain our CLIA certification, and such certification is also required to obtain payment from Medicare, Medicaid and certain other third-party payers. Failure to comply with CLIA or state law requirements may result in the imposition of corrective action or the suspension or revocation of our CLIA certification or state licenses. If our CLIA certification or state licenses are suspended or revoked or our right to bill the Medicare and Medicaid programs or other third-party payers is suspended, we would no longer be able to sell our tests, which would adversely affect our business, financial condition and results of operations.

In addition, no assurance can be given that Aspira Labs' suppliers or commercial partners will remain in compliance with applicable CLIA and other federal or state regulatory requirements for laboratory operations and testing. Aspira Labs' facilities and procedures and those of Aspira Labs' suppliers and commercial partners are subject to ongoing regulation, including periodic inspection by regulatory and other government authorities. The principal sanction under CLIA is suspension, limitation or revocation of a lab's CLIA certificate. CMS also may impose the following alternative sanctions: (a) directed plan of correction, (b) state onsite monitoring, and/or (c) civil monetary penalty. In addition, the government may bring suit to enjoin any activity of any laboratory that has been found with deficiencies during a survey if CMS has reason to believe that continuation of the activity would constitute a significant hazard to the public health. Finally, criminal sanctions may be imposed on an individual who is convicted of intentionally violating any CLIA requirement.

Our clinical laboratory business is also subject to regulation at both the federal and state level in the United States, as well as regulation in other jurisdictions outside of the United States, including:

● Medicare and Medicaid coverage, coding and payment regulations applicable to clinical laboratories;

● The Federal Anti-Kickback Statute, the Eliminating Kickbacks in Recovery Act and state anti-kickback prohibitions;

● the federal physician self-referral prohibition, commonly known as the Stark Law, and state self-referral prohibitions;

● the Medicare civil monetary penalty and exclusion penalty;

● the Federal False Claims Act civil and criminal penalties and state equivalents;

● the federal fraud, waste and abuse laws and state equivalents;

● the federal Physician Payments Sunshine Act; and

● the Federal Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), as amended by the Health Information Technology for Economic and Clinical Health Act of 2009 ("HITECH").

Many of these laws and regulations prohibit a laboratory from making payments or furnishing other benefits to influence the referral of tests (by physicians or others) that are billed to Medicare, Medicaid or certain other federal or state healthcare programs. The penalties for violation of these laws and regulations may include monetary fines, criminal and civil penalties and/or suspension or exclusion from participation in Medicare, Medicaid and other federal healthcare programs. Several states have similar laws that may apply even in the absence of government payers. HIPAA and HITECH

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and similar state laws seek to protect the privacy and security of individually identifiable health information, and penalties for violations of these laws may include required reporting of breaches, monetary fines and criminal or civil penalties.

In 2020, Congress passed the Consolidated Appropriations Act and included a section called the "No Surprises Act." The No Surprises Act prohibits a health care provider from billing a commercially insured patient more than in-network cost-sharing amounts when a service originated from an in-network hospital or ambulatory surgery center, even if the provider is out-of-network with the patient's health plan. It also requires a provider to provide a good faith estimate of expected charges to an uninsured or self-pay patient upon the patient's request or when a patient schedules a service. Several states have similar laws that aim to protect patients from unexpected health care charges. Civil penalties of up to $10,000 per occurrence can be imposed for knowing violations of the No Surprises Act that are not remediated within a certain timeframe, and states may impose their own penalties for violations of their surprise billing laws.

While we seek to conduct our business in compliance with all applicable laws and develop compliance policies to address risk as appropriate, many of the laws and regulations applicable to us are vague or indefinite and have not been interpreted by governmental authorities or the courts. These laws or regulations also could in the future be interpreted or applied by governmental authorities or the courts in a manner that could require us to change our operations.

Any action brought against us for violation of these or other laws or regulations (including actions brought by private *qui tam* "whistleblower" plaintiffs), even if successfully defended, could divert management's attention from our business, damage our reputation, limit our ability to provide services, decrease demand for our services and cause us to incur significant expenses for legal fees and damages. If we fail to comply with applicable laws and regulations, we could suffer significant civil, criminal and administrative penalties, fines, recoupment of funds received by us, exclusion from participation in federal or state healthcare programs, and the loss of various licenses, accreditations, certificates and authorizations necessary to operate our business , contractual damages, reputational harm, diminished profits and future earnings, additional reporting obligations and oversight if we become subject to a corporate integrity agreement or other agreement. We also could potentially incur additional liabilities from third-party claims. If any of the foregoing were to occur, it could have a material adverse effect on our business, financial condition and results of operations.

#### Our ability to utilize our net operating loss carryforwards and certain other tax attributes may be limited.
We have significant net operating loss ("NOL") carryforwards as of December 31, 2025 which are subject to a full valuation allowance due to our history of operating losses. Section 382 of the Internal Revenue Code of 1986, as amended ("Section 382"), as well as similar state provisions restrict our ability to use our NOL carryforwards to offset taxable income due to ownership change limitations that have occurred in the past or that could occur in the future. These ownership changes also may limit the amount of tax credit carryforwards that can be utilized annually to offset future tax liabilities.

Our pre- 2018 federal NOLs will expire in varying amounts from 2025 through 2037, if not utilized, and can offset 100% of future taxable income for regular tax purposes. Any federal NOLs arising on or after January 1, 2018, can be carried forward indefinitely but such federal NOL carryforwards are permitted to be used in any taxable year to offset only up to 80% of taxable income in such year. Portions of our state NOLs will expire in varying amounts from 2025 through 2044 if not utilized. Our ability to use our NOLs during this period will be dependent on our ability to generate taxable income, and portions of our NOLs could expire before we generate sufficient taxable income.

We believe we have experienced ownership changes in the past for purposes of these limitations, and we estimate that a substantial portion of our existing federal NOL and tax credit carryforwards are subject to annual limitation. Additional issuances or sales of our common stock, and certain other transactions involving our stock that are outside of our control, could cause additional ownership changes. Any current or future limitations on the use of our NOLs or tax credit carryforwards could, depending on the extent of such limitation, result in our retaining less cash during any year in which we have taxable income than we would be entitled to retain if such limitations did not apply, which could adversely impact our results of operations and financial condition.

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***If we are able to successfully reinstate the ARPA-H award and yet are unable to complete the required milestones under our federal award milestone-based funding agreement, our business, results of operations and financial condition would be adversely affected.***

On October 23, 2024, we announced that we had been selected by the federal government's Advanced Research Projects Agency for Health ("ARPA-H") as an awardee of a milestone-based funding agreement. On June 9, 2025, we received a notice from ARPA-H that our ENDOinform contract was terminated for failure to meet the specifications of Milestone 3. The loss of this non-dilutive funding eliminates a planned source of support for development activities and may delay the timeline to commercialize ENDOinform unless we are able to reinstate the award or secure alternative financing.

#### Failure to renew our Quest Diagnostics agreement could impact our business
***Our current agreement with Quest Diagnostics expired as of December 31, 2025. If we fail to renew the agreement on acceptable terms, we could experience a disruption in logistics as well as a loss of revenue.***

#### RISKS RELATED TO INTELLECTUAL PROPERTY AND PRODUCT LIABILITY
***If we fail to maintain our rights to utilize intellectual property directed to diagnostic biomarkers, we may not be able to offer diagnostic tests using those biomarkers.***

One aspect of our business plan is to develop diagnostic tests based on certain biomarkers, which we have the right to utilize through licenses with our academic collaborators, such as the Johns Hopkins University School of Medicine, the University of Texas M.D. Anderson Cancer Center, Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz. In some cases, our collaborators own the entire right to the biomarkers. In other cases, we co-own the biomarkers with our collaborators. If, for any reason, we lose our license to biomarkers owned entirely by our collaborators, we may not be able to use those biomarkers in diagnostic tests. If we lose our exclusive license to biomarkers co-owned by us and our collaborators, our collaborators may license their share of the intellectual property to a third party that may compete with us in offering diagnostic tests, which would materially adversely affect our business, results of operations and financial condition.

***If a third party infringes on our proprietary rights, we may lose any competitive advantage we have as a result of diversion of our time, enforcement costs and the loss of the exclusivity of our proprietary rights.***

Our success depends in part on our ability to maintain and enforce our proprietary rights. We rely on a combination of patents, trademarks, copyrights and trade secrets to protect our technology and brand. We have submitted a number of patent applications covering biomarkers that may have diagnostic or therapeutic utility. Our patent applications may or may not result in additional patents being issued.

If third parties engage in activities that infringe on our proprietary rights, we may incur significant costs in asserting our rights, and the attention of our management may be diverted from our business. We may not be successful in asserting our proprietary patient rights, which could result in our patents being held invalid or a court holding that the competitor is not infringing, either of which may harm our competitive position. We cannot be sure that competitors will not design around our patented technology. We also may not be successful in asserting our proprietary trademark rights, which could result in significant rebranding costs, not being able to obtain a federal trademark registration, or a court holding that the competitor is not infringing, any of which may harm our competitive position. We cannot be sure that competitors will not use a similar mark.

We also rely upon the skills, knowledge and experience of our technical personnel. To help protect our rights, we require all employees and consultants to enter into confidentiality agreements that prohibit the disclosure of confidential information. These agreements may not provide adequate protection for our trade secrets, knowledge or other proprietary

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information in the event of any unauthorized use or disclosure. If any trade secret, knowledge or other technology not protected by a patent were to be disclosed to or independently developed by a competitor, it could have a material adverse effect on our business, consolidated results of operations and financial condition.

***If others successfully assert their proprietary rights against us, we may be precluded from making and selling our products or we may be required to obtain licenses to use their technology.***

Our success depends on avoiding infringing on the proprietary technologies of others. If a third party were to assert claims that we are violating its patents, we might incur substantial costs defending ourselves in lawsuits against charges of patent infringement or other allegations of unlawful use of another's proprietary technology. Any such lawsuit may involve considerable management and financial resources and may not be decided in our favor. If we are found liable, we may be subject to monetary damages or an injunction prohibiting us from using the technology. We may also be required to obtain licenses under patents owned by third parties and such licenses may not be available to us on commercially reasonable terms, if at all.

If a third party were to assert claims that we are violating its trademarks, we might incur substantial costs defending ourselves in lawsuits against charges of trademark infringement. Any such lawsuit may involve considerable management and financial resources and may not be decided in our favor. If we are found liable, we may be subject to monetary damages or an injunction prohibiting us from using the mark. We may also be required to rebrand or enter into a co-existence agreement with a third party, which may be commercially restrictive or unreasonable.

#### Our diagnostic efforts may cause us to have significant product liability exposure.
The testing, manufacturing and marketing of medical diagnostic tests entail an inherent risk of product liability claims. Potential product liability claims may exceed the amount of our insurance coverage or may be excluded from coverage under the terms of the policy. We will need to increase the amount of our insurance coverage in the future if we are successful at introducing new diagnostic products, and this will increase our costs. If we are held liable for a claim or for damages exceeding the limit of our insurance coverage, we may be required to make substantial payments. This may have an adverse effect on our business, financial condition and results of operations.

#### Certain of our patent registrations will expire, which may cause us to have significant competition.
Our success depends in part on our ability to own and assert our patent registrations to maintain and enforce our proprietary rights, including defending against infringement actions. We have some patent registrations covering biomarkers that may be expiring, and our strategy to continue to seek protection and file patent applications may or may not result in additional patents being issued.

If any such patent registration is no longer protectable and could be exploited by a competitor, it could have a material adverse effect on our business, consolidated results of operations and financial condition.

#### OPERATIONAL RISKS

#### Because our business is highly dependent on key executives and employees, our inability to recruit and retain these people could hinder our business plans.
We are highly dependent on our executive officers and certain key employees. Our executive officers and key employees are employed at will by us. Any inability to engage new executive officers or key employees could impact operations or delay or curtail our research, development and commercialization objectives. To continue our research and product development efforts, we need people skilled in areas such as clinical operations, regulatory affairs and clinical diagnostics. Competition for qualified employees is intense. To continue our commercialization objectives and reach our financial and operational goals, we require skilled sales individuals with familiarity in our industry. We have from time to time experienced, and may in the future experience, shortages of certain types of qualified employees.

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If we lose the services of any executive officers or key employees, our ability to achieve our business objectives could be harmed, which in turn could adversely affect our business, financial condition and results of operations. We have and may continue to experience turnover in certain executive officer and key employee roles.

#### Business interruptions could limit our ability to operate our business.
Our operations, as well as those of the collaborators on which we depend, are vulnerable to damage or interruption from fire, natural disasters, including earthquakes, weather related supply chain delivery disruptions, computer viruses, cyber-attacks, human error, power shortages, telecommunication failures, international acts of terror, foreign or domestic conflicts, epidemics or pandemics such as the COVID-19 pandemic, and other similar events. Although we have certain business continuity plans in place, we have not established a formal comprehensive disaster recovery plan, and our back-up operations and business interruption insurance may not be adequate to compensate us for losses we may suffer. A significant business interruption could result in losses or damages incurred by us and require us to cease or curtail our operations.

***The operation of Aspira Labs and our Aspira Synergy business depends on the effectiveness and availability of our information systems, including the information systems we use to provide services to our customers and to store employee data. If our information technology systems or those third parties upon which we rely or our data, are or were compromised, we could experience adverse consequences resulting from such compromise, including but not limited to regulatory investigations or actions; litigation; fines and penalties; disruptions of our business operations; reputational harm; loss of revenue or profits; loss of customers or sales; and other adverse consequences.***

In the ordinary course of our business, we and the third parties upon which we rely, process, collect, receive, store, use, transfer, make accessible, and share (collectively, processing) proprietary, confidential, and sensitive data, including personal data (such as health-related data), intellectual property, trade secrets and other sensitive data the Company may process (collectively, sensitive information).

The information systems we use for our Aspira Labs business are comprised of systems we have purchased or developed, our legacy information systems and, increasingly, web-enabled and other integrated information systems. In using these information systems, we may rely on third-party vendors to provide hosting services, where our infrastructure is dependent upon the reliability of their underlying platforms, facilities and communications systems.

As the breadth and complexity of Aspira Labs' information system grows, we will be increasingly exposed to the risks inherent in maintaining the stability of our legacy systems due to prior customization, attrition of employees or vendors involved in their development, and obsolescence of the underlying technology as well as risks from the increasing number and scope of external data breaches on companies generally. Because certain customers and clinical trials may be dependent upon these legacy systems, we will also face an increased level of risk in maintaining the legacy systems and limited options to mitigate such risk. We are also exposed to risks associated with the availability of all of our information systems, including

● discontinued vendor support of legacy systems;

● disruption, impairment or failure of data centers, telecommunications facilities or other key infrastructure platforms, including those maintained by third-party vendors;

● failures or malfunctions in our internal systems, including our employee data and communications, critical application systems and their associated hardware; and

● excessive costs, excessive delays and other deficiencies in systems development and deployment.

Cyber-attacks, malicious internet-based activity, online and offline fraud, social-engineering attacks (including through deep fakes, which may be increasingly more difficult to identify as fake, and phishing attacks), malicious code (such as viruses and worms), malware (including as a result of advanced persistent threat intrusions), denial-of-service attacks, credential stuffing, credential harvesting, personnel misconduct or error, ransomware attacks, supply-chain attacks,

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software bugs, server malfunctions, software or hardware failures, loss of data or other information technology assets, adware, attacks enhanced or facilitated by AI, telecommunications failures, and other similar activities or incidents threaten the confidentiality, integrity, and availability of our sensitive information and information technology systems, and those of the third parties upon which we rely. Such threats are prevalent and continue to rise, are increasingly difficult to detect, and come from a variety of sources, including traditional computer "hackers," threat actors, "hacktivists," organized criminal threat actors, personnel (such as through theft or misuse), sophisticated nation states, and nation-state-supported actors. In addition to experiencing a security incident, third parties may gather, collect, or infer sensitive information about us from public sources, data brokers, or other means that reveals competitively sensitive details about our organization and could be used to undermine our competitive advantage or market position.

The materialization of any of these risks may impede the processing of data, the delivery of databases and services, and the day-to-day management of our Aspira Labs business and could result in the corruption, loss or unauthorized disclosure of proprietary, confidential or other data. In particular, severe ransomware attacks are becoming increasingly prevalent and can lead to significant interruptions in our operations, ability to provide our products or services, loss of sensitive data and income, reputational harm, and diversion of funds. Extortion payments may alleviate the negative impact of a ransomware attack, but we may be unwilling or unable to make such payments due to, for example, applicable laws or regulations prohibiting such payments. Further, remote work has become more common and has increased risks to our information technology systems and data, as more of our employees utilize network connections, computers and devices outside our premises or network, including working at home, while in transit and in public locations.

Our mitigation efforts to date might not adequately protect us in the event of a system failure, cyber-attack, cyber-breach, data breach or other adverse event. Despite any precautions we take, damage from fire, floods, hurricanes, the outbreak or escalation of war, acts of terrorism, power loss, telecommunications failures, computer viruses, break-ins and similar events at our various computer facilities or those of our third-party vendors could result in interruptions in the flow of data to us and from us to our customers. Corruption or loss of data may result in the need to repeat a trial at no cost to the customer, but at significant cost to us, the termination of a contract or damage to our reputation. As our business continues its efforts to expand globally, these types of risks may be further increased by instability in the geopolitical climate of certain regions, underdeveloped and less stable utilities and communications infrastructure, and other local and regional factors. Additionally, significant delays in system enhancements or inadequate performance of new or upgraded systems could damage our reputation and harm our business.

Unauthorized disclosure of sensitive or confidential data, whether through systems failure or employee or distributor negligence, cyber-attacks, fraud or misappropriation, could damage our reputation and cause us to lose customers and, to the extent any such unauthorized disclosure compromises the privacy and security of individually identifiable health information, could also cause us to face sanctions and fines under HIPAA of 1996 as amended by HITECH. Similarly, we have been and expect that we will continue to be subject to attempts to gain unauthorized access to or through our information systems or those we internally or externally develop for our customers, including a cyber-attack by computer programmers and hackers who may develop and deploy viruses, worms or other malicious software programs, process breakdowns, denial-of-service attacks, malicious social engineering or other malicious activities, or any combination of the foregoing. These same risks also apply to Aspira Labs. Successful attacks could result in negative publicity, significant remediation and recovery costs, legal liability and damage to our reputation and could have an adverse effect on our business, financial condition and results of operations.

We use AI/ML to assist us in making certain decisions, which is regulated by certain privacy laws. Due to inaccuracies or flaws in the inputs, outputs, or logic of the AI/ML, the model could be biased and could lead us to make decisions that could bias certain individuals (or classes of individuals), and adversely impact their rights, employment, and ability to obtain certain pricing, products, services, or benefits.

Our contracts may not contain limitations of liability, and even where they do, there can be no assurance that limitations of liability in our contracts are sufficient to protect us from liabilities, damages, or claims related to our data privacy and security obligations. We cannot be sure that our insurance coverage will be adequate or sufficient to protect us from or to mitigate liabilities arising out of our privacy and security practices, that such coverage will continue to be available on commercially reasonable terms or at all, or that such coverage will pay future claims.

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***We selectively explore acquisition opportunities and strategic alliances relating to other businesses, products or technologies. We may not be successful in integrating other businesses, products or technologies with our business. Any such transaction also may not produce the results we anticipate, which could adversely affect our business, financial condition and results of operations.***

We selectively explore and may pursue acquisition and other opportunities to strengthen our business and grow our company. We may enter into business combination transactions, make acquisitions or enter into strategic partnerships, joint ventures or alliances, any of which may be material. The market for acquisition targets and strategic alliances is highly competitive, which could make it difficult to find appropriate merger or acquisition opportunities. If we are required to raise capital by incurring debt or issuing additional equity for any reason in connection with a strategic acquisition or investment, financing may not be available or the terms of such financing may not be favorable to us and our stockholders, whose interests may be diluted by the issuance of additional stock.

The process of integration may produce unforeseen regulatory issues and operating difficulties and expenditures and may divert the attention of management from the ongoing operation of our business and harm our reputation. We may not successfully achieve the integration objectives, and we may not realize the anticipated cost savings, revenue growth and synergies in full or at all, or it may take longer to realize them than expected, any of which could negatively impact our business, financial condition and results of operations.

#### Future litigation by or against us could be costly and time-consuming to prosecute or defend.
We are from time to time subject to legal proceedings and claims that arise in the ordinary course of business, such as claims brought by our clients in connection with commercial disputes, employment claims made by current or former employees, and claims brought by third parties alleging infringement of their intellectual property rights. In addition, we may bring claims against third parties for infringement of our intellectual property rights. Litigation may result in substantial costs and may divert our attention and resources, which may adversely affect our business, results of operations and financial condition.

An unfavorable judgment against us in any legal proceeding or claim could require us to pay monetary damages. In addition, an unfavorable judgment in which the counterparty is awarded equitable relief, such as an injunction, could harm our business, results of operations and financial condition.

#### RISKS RELATED TO OWNING OUR STOCK

#### There is a limited market for our Common Stock.
Our common stock is not listed on any national exchange. We were delisted from The Nasdaq Capital Market on April 15, 2025. Our common stock immediately began trading on the OTC Markets Group and currently trade on the OTC Markets OTCQX system. As such, owners of our common stock experience reduced liquidity and limited availability of market quotations for our securities. Our access to capital and facilities such as an At-the-Market or equity line of credit that require a national exchange listing could be impaired. Owners of our stock may be impacted by the loss of institutional investor interest, the limited amount of analyst coverage and the requirement for brokers trading in our common stock to adhere to more stringent "penny-stock" rules, which could possibly result in a reduced level of trading activity for our securities.

***If we fail to maintain compliance with the OTC QX minimum listing requirements, our common stock will be subject to removal to the OTC QB market. Our ability to publicly or privately sell equity securities and the liquidity of our common stock could be adversely affected if our common stock is downgraded.***

The continued trading of our common stock on The OTC QX Best Market is contingent on our continued compliance with a number of listing requirements. If we are unable to comply with the continued listing requirements of The OTC QX Best Market, our common stock would be downgraded to the OTC QB Market, which would further limit investors' ability to effect transactions in our common stock and subject us to additional trading restrictions. In order to

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maintain our trading status, we must maintain certain share prices, financial and share distribution targets, including maintaining a minimum amount of revenue, assets or market capitalization.

There is no assurance that we will be able to maintain compliance with The OTC QX Best Market continued trading standards in the future.

***The liquidity and trading volume of our common stock may be low, and our ownership is concentrated, which could adversely impact the trading price of our common stock and our stockholders' ability to obtain liquidity.***

The liquidity and trading volume of our common stock has at times been low in the past and may again be low in the future. If the liquidity and trading volume of our common stock is low, this could adversely impact the trading price of our common stock and our stockholders' ability to obtain liquidity in their shares of our common stock.

In addition, pursuant to a stockholders agreement we entered into in connection with a May 2013 private placement (the "2013 Stockholders Agreement"), one of our stockholders has the right to designate a director to be nominated by us to serve on our board of directors. Furthermore, this Stockholders Agreement gives two investors the right to participate in future equity offerings, on the same terms as other investors. In addition, the Stockholders Agreement prohibits us from taking certain material actions without the consent of at least one of the primary investors in the May 2013 private placement. These material actions include:

● making any acquisition with a value greater than $2 million;

● offering, selling or issuing securities senior to our common stock or any securities that are convertible into or exchangeable or exercisable for securities ranking senior to our common stock;

● taking any action that would result in a change in control of the Company or an insolvency event; and

● paying or declaring dividends on any of our securities or distributing any of our assets other than in the ordinary course of business or repurchasing any of our outstanding securities.

The foregoing rights terminate for a primary investor when that investor ceases to beneficially own less than 50% of the shares and warrants (taking into account shares issued upon exercise of the warrants), in the aggregate, that were purchased at the closing of the 2013 private placement. We believe that the rights of one of the primary investors have terminated. The interests of the parties to the 2013 Stockholders Agreement could conflict with or differ from our interests or the interests of other stockholders.

As a result of the foregoing, a limited number of stockholders will be able to affect the outcome of, or exert significant influence over, all matters requiring stockholder approval, including the election and removal of directors and any change in control involving us. In addition, this concentration of ownership of our common stock could have the effect of delaying or preventing a change in control of us or otherwise discouraging or preventing a potential acquirer from attempting to obtain control of us. This, in turn, could have a negative effect on the market price of our common stock. It could also prevent our stockholders from realizing a premium over the market prices for their shares of common stock. Moreover, the interests of this concentration of ownership may not always coincide with our interests or the interests of other stockholders. In addition, the interests of the parties to the Stockholders Agreement could conflict with or differ from our interests or the interests of other stockholders. The concentration of ownership also contributes to the low trading volume and volatility of our common stock.

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#### Our stock price has been, and may continue to be, highly volatile.
The trading price of our common stock has been highly volatile. Between January 1, 2025, and December 31, 2025, the closing trading price of our common stock ranged from $0.75 to $0.02. The trading price of our common stock could continue to be subject to wide fluctuations in price in response to various factors, many of which are beyond our control, including:

● failure to significantly increase revenue and volumes of OvaSuite or Aspira Synergy;

● actual or anticipated period-to-period fluctuations in financial results;

● failure to achieve, or changes in, financial estimates by securities analysts;

● announcements or introductions of new products or services or technological innovations by us or our competitors;

● failure to complete clinical studies that validate clinical utility sufficiently to increase positive medical policy among payers at large;

● publicity regarding actual or potential discoveries of biomarkers by others;

● comments or opinions by securities analysts or stockholders;

● conditions or trends in the pharmaceutical, biotechnology or life science industries;

● announcements by us of significant acquisitions and divestitures, strategic partnerships, joint ventures or capital commitments;

● developments regarding our patents or other intellectual property or that of our competitors;

● litigation or threat of litigation;

● additions or departures of key personnel;

● limited daily trading volume;

● our ability to continue as a going concern;

● economic and other external factors, disasters or crises; and

● our announcement of future fundraisings.

In addition, the stock market in general and the market for diagnostic technology companies, in particular, have experienced significant price and volume fluctuations that have often been unrelated or disproportionate to the operating performance of those companies. These broad market and industry factors may adversely affect the market price of our securities, regardless of our operating performance. In the past, following periods of volatility in the market price of a company's securities, securities class action litigation has often been instituted. A securities class action suit against us could result in substantial costs, potential liabilities and the diversion of our attention and our resources.

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#### Anti-takeover provisions in our charter, bylaws, other agreements and under Delaware law could make a third-party acquisition of the Company difficult.
Certain provisions of our certificate of incorporation and bylaws may have the effect of making it more difficult for a third party to acquire, or of discouraging a third party from attempting to acquire, control of us, even if a change of control might be deemed beneficial to our stockholders. Such provisions could limit the price that certain investors might be willing to pay in the future for our securities. Our certificate of incorporation eliminates the right of stockholders to call special meetings of stockholders or to act by written consent without a meeting, and our bylaws require advance notice for stockholder proposals and director nominations, which may preclude stockholders from bringing matters before an annual meeting of stockholders or from making nominations for directors at an annual meeting of stockholders. Our certificate of incorporation authorizes undesignated preferred stock, which makes it possible for our board of directors, without stockholder approval, to issue preferred stock with voting or other rights or preferences that could adversely affect the voting power of holders of common stock. In addition, the likelihood that the holders of preferred stock will receive dividend payments and payments upon liquidation could have the effect of delaying, deferring or preventing a change in control.

In connection with our private placement offering of common stock and warrants in May 2013 we entered into the 2013 Stockholders Agreement which, among other things, includes agreements limiting our ability to effect a change in control without the consent of at least one of the primary investors in that offering. These and other provisions may have the effect of deferring hostile takeovers or delaying changes in control or management of us. The amendment of any of the provisions of either our certificate of incorporation or bylaws described in the preceding paragraph would require not only approval by our board of directors and the affirmative vote of at least 66 2/3% of our then outstanding voting securities, but also consent pursuant to the terms of the 2013 Stockholders Agreement. We are also subject to certain provisions of Delaware law that could delay, deter or prevent a change in control of the Company. These provisions could make a third-party acquisition of the Company difficult and limit the price that investors might be willing to pay in the future for shares of our common stock.

#### If we raise additional capital in the future, your ownership in us could be diluted.
In order to raise additional capital, we may offer additional shares of common stock or other securities convertible into or exchangeable for our common stock. We may sell shares or other securities in any other offering at a price per share that is less than the price for securities paid by investors in previous offerings, and investors purchasing shares or other securities in the future could have rights superior to existing stockholders. The price per share at which we sell additional shares of common stock or securities convertible into common stock in future transactions may be higher or lower than the price for securities offered in previous offerings.

Until such time, if ever, as we can generate substantial revenue from our operations, we anticipate financing our cash needs through a combination of equity offerings, debt financings and license agreements. To the extent that we raise additional capital through the further sale of equity securities or convertible debt securities, your ownership interest will be diluted.

#### Sales of a substantial number of our shares of common stock in the public market could cause our stock price to fall.
We may issue and sell additional shares of common stock in the public markets. Sales of a substantial number of shares of our common stock in the public markets or the perception that such sales could occur could depress the market price of our securities and impair our ability to raise capital through the sale of additional equity securities.

***Because we do not currently intend to declare cash dividends on our shares of common stock in the foreseeable future, stockholders must rely on appreciation of the value of our common stock for any return on their investment.***

We have never declared or paid cash dividends on our capital stock. We currently intend to retain all of our future earnings, if any, to finance the operation, development and growth of our business. Furthermore, any future debt agreements may also preclude us from paying or place restrictions on our ability to pay dividends. As a result, capital

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appreciation, if any, of our common stock will be your sole source of gain with respect to your investment for the foreseeable future.

#### The exercise of our outstanding options and warrants will dilute stockholders and could decrease our stock price.
The exercise of our outstanding options and warrants may adversely affect our stock price due to sales of a large number of shares or the perception that such sales could occur. These factors also could make it more difficult to raise funds through future offerings of our securities, and could adversely impact the terms under which we could obtain additional equity capital. Exercise of outstanding options and warrants or any future issuance of additional shares of common stock or other equity securities, including, but not limited to, options, warrants, restricted stock units or other derivative securities convertible into our common stock, may result in significant dilution to our stockholders and may decrease our stock price.

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| **ITEM 1B.** | **UNRESOLVED STAFF COMMENTS** |

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None.

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| **ITEM 1C.** | **CYBERSECURITY** |

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#### Risk Management and Strategy
We have implemented and maintain various information security processes designed to identify, assess and manage material risks from cybersecurity threats to our critical computer networks, third party hosted services, communications systems, hardware and software, and our critical data, including intellectual property, confidential information that is proprietary, strategic or competitive in nature, and trade secrets, data we may collect about trial participants in connection with clinical trials, sensitive third-party data, business plans, transactions, and financial information ("Information Systems and Data").

Our cybersecurity function, which comprises, in part, our IT department, legal team, human resources team and our audit committee, helps identify, assess and manage our cybersecurity threats and risks. Our cybersecurity function identifies and assesses risks from cybersecurity threats by monitoring and evaluating our threat environment using various methods including, for example, automated tools, subscribing to and analyzing reports and services that identify cybersecurity threats, conducting vulnerability assessments to identify vulnerabilities, and evaluating threats reported to us.

Depending on the environment, we implement and maintain various technical, physical, and organizational measures and processes designed to manage and mitigate material risks from cybersecurity threats to our Information Systems and Data, including, for example: incident detection and response, data encryption, network security controls, employee training, access controls, physical security, systems monitoring, and asset management, tracking, and disposal.

Our assessment and management of material risks from cybersecurity threats are integrated into our overall risk management processes. For example, the cybersecurity function works with management to prioritize our risk management processes and mitigate cybersecurity threats that are more likely to lead to a material impact to our business.

We use third-party service providers to assist us from time to time to identify, assess, and manage material risks from cybersecurity threats, including for example professional services firms (including legal counsel) and cybersecurity consultants. We also use third-party service providers to perform a variety of functions throughout our business, such as hosting companies, application providers, and supply chain resources. We manage cybersecurity risks associated with our use of these providers by, for example, requesting and analyzing responses on a security questionnaire and conducting audits and risk assessments on certain vendors. In particular, our legal department performs an assessment on each vendor and based on certain criteria will have our IT team perform a security assessment.

For a description of the risks from cybersecurity threats that may materially affect the Company and how they may do so, see our risk factors under Part 1. Item 1A. Risk Factors in this Annual Report on Form 10-K, including *"The* 

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*operation of Aspira Labs and our Aspira Synergy business depends on the effectiveness and availability of our information systems, including the information systems we use to provide services to our customers and to store employee data, and failures of these systems, including in connection with cyber-attacks, may materially limit our operations or have an adverse effect on our reputation."*

#### Governance
Our board of directors addresses our cybersecurity risk management as part of its general oversight function. The Audit Committee is responsible for overseeing our cybersecurity risk management processes, including oversight and mitigation of risks from cybersecurity threats.

Our cybersecurity risk assessment and management processes are implemented and maintained by certain members of our management, including our Manager of IT Infrastructure, who has over 20 years of experience in various IT administration roles, five of which have been in cybersecurity.

Our HR department is responsible for hiring appropriate personnel, helping to integrate cybersecurity risk considerations into our overall risk management strategy, and communicating key priorities to relevant personnel. Our CFO, working with our Manager of IT Infrastructure, is responsible for approving budgets, helping prepare for cybersecurity incidents, approving cybersecurity processes, and reviewing security assessments and other security-related reports. Our legal department is also responsible for performing a cyber risk assessment on each new vendor.

Our response process to cybersecurity incidents is designed to escalate certain incidents to members of management depending on the circumstances, including our Manager of IT Infrastructure. Our Manager of IT Infrastructure works with our incident response team to help us to mitigate and remediate cybersecurity incidents of which they are notified. In addition, our incident response policy includes reporting to the board of directors committee responsible for certain cybersecurity incidents.

The Audit Committee receives periodic reports from our cybersecurity function concerning our significant cybersecurity threats and risk and the processes we have implemented to address them. The Audit Committee also has access to various reports, summaries or presentations related to cybersecurity threats, risk and mitigation.

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| | |
|:---|:---|
| **ITEM 2.** | **PROPERTIES** |

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The following chart indicates the facilities that we lease, the location and size of each facility and its designated use. We believe that these facilities are suitable and adequate for our current needs.

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| | | | |
|:---|:---|:---|:---|
| **Location** | **Approximate Square Feet** | **Primary Functions** | **Lease Expiration Date** |
| Austin, Texas | 8,203 sq. ft. | Aspira Labs facility, research and development, clinical and regulatory and administrative offices | August 31, 2031 |
| Shelton, Connecticut | 4,614 sq. ft. | Administrative offices | September 30, 2028 |

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|:---|:---|
| **ITEM 3.** | **LEGAL PROCEEDINGS** |

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From time to time, we are involved in legal proceedings and regulatory proceedings arising out of our operations. We establish reserves for specific liabilities in connection with legal actions that we deem to be probable and estimable. As of the date of the filing of this Form 10-K, we are not a party to any proceeding, the adverse outcome of which would have a material adverse effect on our financial position or results of operations.

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| | |
|:---|:---|
| **ITEM 4.** | **MINE SAFETY DISCLOSURES** |

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Not applicable.

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#### PART II

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|:---|:---|
| **ITEM 5.** | **MARKET FOR REGISTRANT'S COMMON EQUITY, RELATED STOCKHOLDER MATTERS AND ISSUER PURCHASES OF EQUITY SECURITIES** |

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#### Market Information
Our common stock is traded on The OTCQX Market under the symbol "AWHL."

#### Holders of Common Stock
On March 30, 2026, there were 66 registered holders of record of our common stock.

#### Dividends
We have never paid or declared any dividend on our common stock and we do not anticipate paying cash dividends on our common stock in the foreseeable future. If we pay a cash dividend on our common stock, we also may be required to pay the same dividend on an as-converted basis on any outstanding warrants or other securities. Moreover, any preferred stock or other senior debt or equity securities to be issued and any future credit facilities might contain restrictions on our ability to declare and pay dividends on our common stock. We intend to retain all available funds and any future earnings to fund the development and expansion of our business.

#### Recent Sales of Unregistered Securities
None.

#### Issuer Purchases of Equity Securities
None.

#### Equity Compensation Plan Information
Information about our equity compensation plans is incorporated herein by reference to Item 12 of Part III of this Annual Report on Form 10-K

#### Stock Performance Graph
We are a smaller reporting company as defined by Rule 12b-2 of the Exchange Act and are not required to provide the information required under this item.

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| | |
|:---|:---|
| **ITEM 6.** | **[Reserved]** |

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|:---|:---|
| **ITEM 7.** | **MANAGEMENT'S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS** |

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*You should read the following discussion and analysis in conjunction with our audited Consolidated Financial Statements and related Notes thereto, included on pages F-1 through F-34 in this Annual Report on Form 10-K. The statements below contain forward-looking statements based upon current plans, expectations, and beliefs that involve risks and uncertainties. Actual results may differ materially from those contained in any forward-looking statement, due to a number of factors, including those discussed in the section of this Annual Report on Form 10-K entitled "Forward-Looking Statements" and "Item 1A. Risk Factors" in this Form 10-K. You should read these sections carefully.*

#### Overview
We are dedicated to the discovery, development, and commercialization of noninvasive, AI-powered tests to aid in the diagnosis of gynecologic diseases, starting with ovarian cancer.

We plan to broaden our focus to the differential diagnosis of other gynecologic diseases that typically cannot be assessed through traditional non-invasive clinical procedures, expanding our addressable market to over 10 million women. We expect to continue commercializing our existing and new technology and to distribute our test through both in-house and distributed pathways. We also intend to continue to raise public awareness regarding the diagnostic superiority of the Ova1Plus workflow as compared to CA-125 on its own for all women with adnexal masses, as well as the superior performance of our tests in detecting ovarian cancer in different racial populations. We plan to continue to expand access to our tests among Medicaid patients as part of our corporate mission to make the best care available to all women, and we plan to advocate for legislation and the adoption of our technology in professional society guidelines to provide broad access to our products and services.

To continue our commercialization objectives and reach our financial and operational goals, we require skilled sales individuals with familiarity in our industry. We have from time to time experienced, and may in the future experience, shortages of certain types of qualified employees.

***2025 Strategic Shift***

At the beginning of 2025, our new management team initiated a decisive strategic shift to position the Company on a path to profitability. This transformation was designed to significantly reduce cash burn while maintaining revenue performance.

As part of this effort, we streamlined our organization, reducing total headcount from 66 full-time employees as of December 31, 2024 to 36 full-time employees and two part-time employees in early 2025. In parallel, we executed a comprehensive reset of our commercial strategy. Leveraging detailed geographic profit and loss analysis, we reallocated resources to regions with favorable reimbursement profiles and reduced or eliminated outside sales efforts in markets with limited or no reimbursement. These actions were reinforced through enhanced sales training and a redesigned compensation structure aligned with profitability objectives.

We also optimized our marketing investments by eliminating spend that was not generating measurable revenue growth and implemented disciplined expense management practices consistent with a more efficient operating model.

To further improve margins, we intensified our focus on higher-reimbursement opportunities and expanded engagement with large accounts, including OB-GYN and hospital networks. This targeted approach resulted in two new contracts and a growing pipeline of additional large customer opportunities. Notably, we executed a contract with Mayo Clinical Laboratories and began generating new 2025 testing volume from Cleveland Clinic Foundation.

Concurrently, management conducted a comprehensive review of vendor and service agreements, resulting in renegotiated terms for key services, the transition to lower-cost vendors while maintaining service levels, and the elimination of non-essential contracts through process improvements.

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As discussed in "Results of Operations," these strategic actions delivered meaningful financial impact. We maintained year-over-year revenue while reducing operating loss by 51% and decreasing cash used in operations by 40%, demonstrating tangible progress toward profitability.

Management will continue to execute on these initiatives, with a focus on cost efficiency, improved average unit pricing for its tests, and increased unit sales. In 2026, we plan to build on this momentum as we advance our path to profitability and achieve key milestones across our product pipeline.

***Product Pipeline***

We aim to introduce new gynecologic diagnostic products and to expand our product offerings to additional women's gynecologic health diseases by adding additional gynecologic bio-analytic solutions involving biomarkers, genetics, clinical risk factors and patient data to aid diagnosis and risk stratification. Future product expansions will be accelerated by the development of lab developed testing in a CLIA environment, relationships with strategic research and development partners, and access to specimens in our biobank.

● **ENDOinform**, a key pipeline development focus, is a multi-marker test program that combines serum proteins, proprietary miRNAs, and clinical data (metadata), for the identification of endometriosis. The initial test development was in collaboration with a consortium of academic and clinical partners: Dana-Farber Cancer Institute (providing clinical and trial design expertise), Brigham & Women's Hospital (providing miRNA technical expertise), and Medical University of Lodz (providing miRNA biomarker and bioinformatics analytic support). Investigations with intended use samples show that serum miRNA and proteins demonstrate favorable analytical properties and sufficient performance to detect of endometriosis cases when analyzed on commercial platforms. Additionally, this data set will provide information on disease classification capability of miRNA and proteins. This is a critical step in evaluating the strength of algorithms that incorporate miRNAs and proteins. The total addressable market is 6.5 million women in the U.S. affected by Endometriosis.

● **OVAinform** is in the development stage and is a multi-marker test that combines serum proteins, proprietary miRNAs and clinical data (metadata) for assessing the risk of ovarian cancer in women with an adnexal mass, with an expanded indication as a surveillance tool for women determined to be at elevated risk of ovarian cancer due to genetic risk or a first degree relative with ovarian cancer, increasing our total addressable market to 4 million women. The discovery work was developed in collaboration with Dana-Farber Cancer Institute (providing clinical and trial design expertise), Brigham & Women's Hospital (providing miRNA technical expertise), and Medical University of Lodz (providing miRNA biomarker and bioinformatics analytic support).

The miRNAs used in the OVAinform test were the subject of a 2017 paper, "*Diagnostic potential for a serum miRNA neural network for detection of ovarian cancer*" published in the peer-reviewed journal Cancer Biology. In November 2024 a peer review journal publication entitled "*Serum miRNA improves the accuracy of a multivariate index assay for triage of an adnexal mass*" was published by our collaborator Dr. Kevin Elias' lab in the journal Gynecologic Oncology. This paper demonstrated that the combination of miRNAs with serum protein biomarkers from Aspiras's ovarian cancer risk tests provided superior performance over existing ovarian cancer risk assessment blood tests.

We have tested our entire set of selected miRNA biomarkers and, based on their performance, we are refining the features on our droplet digital PCR commercial platform. As a next step, we intend to increase our patient sample testing to refine the algorithm for the expanded utility of OVAinform.

#### ARPA-H
On October 23, 2024, the Advanced Research Projects Agency for Health ("ARPA-H") announced that it had selected Aspira as an awardee of the Sprint for Women's Health. As an awardee, we would have received $10,000,000 in funding over two years through the Sprint for Women's Health launchpad track for later-stage health solutions. We were

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entitled to payments based on the completion of certain agreed-upon milestones. The award also provided for access to advisors to support the successful completion and commercial launch of the test before the end of the two-year contract term.

Through our development of ENDOinform, as discussed above, we met the first milestone for payment in the fourth quarter of 2024 and received a payment of $2,000,000. The second milestone was met during the first quarter of 2025, and we received a payment of $1,500,000. These payments are recognized in Other expense (income), net in the consolidated statement of operations for the years ended December 31, 2024 and 2025.

On June 9, 2025, Aspira received notice from VentureWell, the assigned managing contractor for ARPA-H, that Aspira had not met the specifications of the third milestone, and therefore elected to terminate the contract award. We disagree with this finding and believe we have successfully completed the third milestone per the agreement.

After the termination of this award, we have continued to fund ENDOinform development work. We will require additional funding to maintain the working capital necessary to continue our existing operations while also developing our product pipeline.

#### Critical Accounting Estimates
Our significant accounting policies are described in Note 1*, Basis for Presentation and Summary of Significant Accounting and Reporting Policies*, of the Notes to our Consolidated Financial Statements included in this Annual Report on Form 10-K. The Consolidated Financial Statements are prepared in conformity with GAAP. Preparation of the financial statements requires us to make critical judgments, estimates, and assumptions that affect the amounts of assets and liabilities in the financial statements and revenues and expenses during the reporting periods (and related disclosures). We believe the policies discussed below are our critical accounting estimates, as they include the more significant, subjective, and complex judgments and estimates made when preparing our consolidated financial statements.

*Revenue Recognition*

We recognize product revenue in accordance with the provisions of ASC Topic 606, *Revenue from Contracts with Customers* ("ASC 606"); all revenue is recognized upon completion of the OvaSuite tests based on estimates of amounts that will ultimately be realized. In determining the amount to accrue for a delivered test result, we consider factors such as historical payment history and amount, payer coverage, whether there is a reimbursement contract between the payer and us, and any current developments or changes that could impact reimbursement. These estimates are subject to uncertainty and require significant judgment by management because of the various inputs of the factors considered. We also review our patient account population and determine an appropriate distribution of patient accounts by payer (*i.e.*, Medicare, patient pay, other third-party payer, *etc*.) into portfolios with similar collection experience. When evaluated for collectability, this results in a materially consistent revenue amount for such portfolios as if each patient account were evaluated on an individual contract basis.

*Common Stock Warrants*

In August 2022, we entered into an underwriting agreement, pursuant to which we issued warrants to purchase up to 799,985 shares of our Common Stock (the "August 2022 Warrants"). The August 2022 Warrants are classified as a liability on our financial statements and fair value is determined using the Black-Scholes option pricing model. This model uses Level 2 inputs.

In March 2025, we recorded warrants to purchase up to 12,298,177 shares of our Common stock in connection with the conversion of Senior Secured Convertible Promissory Notes (the "March 2025 Warrants"). The March 2025 Warrants are classified as a liability on our financial statements. The March 2025 Warrants had an exercise price of $0.25 per share for the first 24 months after issuance, and $0.50 per share thereafter, but were modified in September 2025 to an exercise price of $0.35 per share (the "Amended March 2025 Warrants"), utilizing a fixed expiration date. Prior to the modification, the fair value of the March 2025 Warrants was estimated using a Monte Carlo simulation pricing model, which uses Level 3 inputs due to its incorporation of significant inputs that are not observable in the market. The mean

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present value across multiple simulation iterations was used to estimate the fair value of the March 2025 Warrants. Following the modification, the fair value of the Amended March 2025 Warrants is determined using the Black-Scholes option pricing model.

Level 2 assumption inputs generally require analysis to develop.

● Expected term – The expected term assumption represents the contractual period of the warrants.

● Volatility – The volatility assumptions are based on the actual historic volatility of our Common Stock over the expected term of the warrants.

● Expected Dividend Yield – The Black-Scholes option-pricing valuation model calls for a single expected dividend yield as an input. We currently have no history or expectation of paying cash dividends on our common stock.

● Risk-Free Interest Rate – The risk-free interest rate is based on the yield available on the U.S. Treasury zero-coupon issues similar in duration to the expected term of the warrants.

Warrants to purchase 450,000 shares of our Common Stock were exercised in September 2025.

*Stock-Based Compensation*

We record the fair value of non-cash stock-based compensation costs for stock options and stock purchase rights related to the 2010 and 2019 Plans. We estimate the fair value of stock options using a Black-Scholes option valuation model. This model requires the input of subjective assumptions including expected stock price volatility, expected life and estimated forfeitures of each award. We use the straight-line method to amortize the fair value over the vesting period of the award. These assumptions consist of estimates of future market conditions, which are inherently uncertain, and therefore are subject to management's judgment.

The expected life of options is based on historical data of our actual experience with the options we have granted and represents the period of time that the options granted are expected to be outstanding. This data includes employees' expected exercise and post-vesting employment termination behaviors. The expected stock price volatility is estimated using our historical volatility in deriving the expected volatility assumption. We made an assessment that our historic volatility is most representative of future stock price trends. The expected dividend yield is based on the estimated annual dividends that we expect to pay over the expected life of the options as a percentage of the market value of our common stock as of the grant date. The risk-free interest rate for the expected life of the options granted is based on the United States Treasury yield curve in effect as of the grant date.

There is inherent uncertainty in our forecasts and projections and, if we had made different assumptions and estimates than those described previously, the amount of our stock-based compensation expense, net loss and net loss per common stock amounts could have been materially different.

*Liquidity*

As discussed in Note 1 to our consolidated financial statements, *Basis for Presentation and Summary of Significant Accounting and Reporting Policies,* we have incurred significant net losses and negative cash flows from operations since inception, and as a result have an accumulated deficit of approximately $544,177,000 at December 31, 2025. We expect to incur a net loss in 2026 as well. In order to continue our operations as currently planned through 2026 and beyond, we will need to raise additional capital. Given the above conditions, there is substantial doubt about our ability to continue as a going concern. The consolidated financial statements have been prepared on a going concern basis and do not include any adjustments that might result from these uncertainties.

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On June 9, 2025, we received a notice from Advanced Research Projects Agency for Health ("ARPA-H") that our ENDOinform contract originally signed in October 2024 was terminated for failure to meet the specifications of the third milestone under the contract. We received $2,000,000 in the fourth quarter of 2024 and $1,500,000 in the first quarter of 2025 prior to the termination of the contract. The loss of this non-dilutive funding eliminates a planned source of support for development activities and may delay the timeline to commercialize ENDOinform unless we are able to reinstate the award or secure alternative financing.

#### Recent Accounting Pronouncements
For additional information, see Note 2 to our consolidated financial statements, *Recent Accounting Pronouncements,* contained in Part II, Item 8, "Consolidated Financial Statements and Supplementary Data," of this Annual Report on Form 10-K.

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#### Results of Operations – Year Ended December 31, 2025 as compared to Year Ended December 31, 2024
Our selected summary financial and operating data for the years ended December 31, 2025 and 2024 were as follows:

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| | | | | |
|:---|:---|:---|:---|:---|
| | **Year Ended**  | **Year Ended**  |  |  |
| | **December 31,**  | **December 31,**  | **Increase (Decrease)** | **Increase (Decrease)** |
| <br>**(dollars in thousands)** | **2025** | **2024** | **Amount** | **%** |
| Revenue: |  |  |  |  |
| &nbsp;&nbsp;Product | $9216 | $9182 | $34 | - |
| &nbsp;&nbsp;&nbsp;&nbsp;Total revenue | 9216 | 9182 | 34 | - |
| Cost of revenue: |  |  |  |  |
| &nbsp;&nbsp;Product | 3301 | 3703 | (402) | (11) |
| &nbsp;&nbsp;&nbsp;&nbsp;Total cost of revenue | 3301 | 3703 | (402) | (11) |
| &nbsp;&nbsp;&nbsp;&nbsp;Gross profit | 5915 | 5479 | 436 | 8 |
| Operating expenses: |  |  |  |  |
| &nbsp;&nbsp;Research and development | 2878 | 3266 | (388) | (12) |
| &nbsp;&nbsp;Sales and marketing | 2986 | 8146 | (5160) | (63) |
| &nbsp;&nbsp;General and administrative | 7964 | 10345 | (2381) | (23) |
| &nbsp;&nbsp;&nbsp;&nbsp;Total operating expenses | 13828 | 21757 | (7929) | (36) |
| Loss from operations | (7913) | (16278) | 8365 | (51) |
| Other income (expense), net: |  |  |  |  |
| &nbsp;&nbsp;Change in fair value of warrant liabilities | (5607) | 1346 | (6953) | (517) |
| &nbsp;&nbsp;Change in fair value of convertible notes | 170 | - | 170 | - |
| &nbsp;&nbsp;Loss upon issuance of Convertible Notes carried at fair value | (1198) | - | (1198) | - |
| &nbsp;&nbsp;Interest expense, net | (41) | (33) | (8) | 24 |
| &nbsp;&nbsp;Other income (expense), net | 1809 | 1871 | (62) | (3) |
| &nbsp;&nbsp;&nbsp;&nbsp;Total other (expense) income, net | (4867) | 3184 | (8051) | (253) |
| Net loss | $(12780) | $(13094) | $314 | (2) |

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***Product Revenue*.** Product revenue was materially unchanged for the year ended December 31, 2025, compared to the same period in 2024. Revenue for Aspira Labs is recognized when the Ova1, Overa, Ova1Plus or OvaWatch test is completed based on estimates of what we expect to ultimately realize. The Company had a 7% reduction in unit sales, however maintained flat revenue through a focus on sales in more profitable regions of the country, resulting in a higher AUP (shown below).

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| | | |
|:---|:---|:---|
|  | **Year Ended**  | **Year Ended**  |
|  | **December 31,**  | **December 31,**  |
|  | **2025** | **2024** |
| Product Volume: |  |  |
| &nbsp;&nbsp;Ova1Plus | 17472 | 19202 |
| &nbsp;&nbsp;OvaWatch | 5043 | 5103 |
| Total OvaSuite | 22515 | 24305 |
| Average Unit Price (AUP): |  |  |
| &nbsp;&nbsp;Ova1Plus | $414 | $382 |
| &nbsp;&nbsp;OvaWatch | 394 | 362 |
| Total OvaSuite | $409 | $378 |

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***Cost of Revenue – Product.*** The decrease in Cost of product revenue was primarily due to a decrease in phlebotomy expenses of $289,000 and personnel costs of $180,000, offset by an increase in consulting costs of $107,000. We expect the cost of revenue to increase slightly in 2026 as the number of tests performed increases.

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***Research and Development Expenses*.** Research and development expenses represent costs incurred to develop our technology and carry out clinical studies, and include personnel-related expenses, regulatory costs, reagents and supplies used in research and development laboratory work, infrastructure expenses, contract services and other outside costs. The decrease in Research and development expenses was primarily due to a reduction in consulting costs of $620,000, personnel expenses of approximately $200,000 and lab supply costs of $104,000, offset by an increase to our clinical trials of $600,000. We expect research and development expenses to increase in 2026 due to the focus on our pipeline.

***Sales and Marketing Expenses.*** Our sales and marketing expenses consist primarily of personnel-related expenses, education and promotional expenses. These expenses include the costs of educating physicians and other healthcare professionals, medical meeting participation, and dissemination of scientific and health economic publications. The decrease in Sales and marketing expenses was primarily due to a decrease in personnel costs of $2,944,000, costs related to our contracted sales team of $972,000, travel expenses of $738,000 and other marketing costs of $297,000. We expect sales and marketing expenses to remain flat for the majority of 2026.

***General and Administrative Expenses*.** General and administrative expenses consist primarily of personnel-related expenses, professional fees, including legal, finance and accounting expenses and other infrastructure expenses. The decrease in General and administrative expenses was primarily due to a decrease in personnel costs of $2,723,000 and outside legal costs of $364,000, offset by increased consulting expenses of $430,000 and accounting costs of $389,000. We expect general and administrative expenses to remain flat during 2026.

***Change in fair value of warrant liabilities.*** For the year ended December 31, 2025, there was a net increase in the Change in fair value of Warrant Liabilities of $5,607,000. The increase was due to the modification of the March 2025 Warrants, as well as the increase in our stock price during the year. For the year ended December 31, 2024, there was a net decrease in fair value of $1,346,000. The change in fair value during the year ended December 31, 2024 was primarily due to a decrease in our stock price during the year.

***Change in fair value of Convertible Notes.*** The change in fair value of Convertible Notes for the year ended December 31, 2025 was $170,000, which was the gain recognized upon the conversion of the Convertible Notes into Units.

***Loss upon issuance of Convertible Notes carried at fair value.*** The loss upon issuance of Convertible Notes carried at fair value for the year ended December 31, 2025 was $1,198,000. This represents an immediate loss recognized by the Company upon the issuance of the Convertible Notes.

***Other Income, net.*** For the year ended December 31, 2025, we recognized Other net income of $1,809,000, comprised of the receipt of $1,500,000 from ARPA-H and the Employee Retention Tax Credits of $1,000,000, offset by $496,000 of transaction costs related to 2025 Lincoln Park Agreement. For the year ended December 31, 2024, we recognized Other net income of $1,871,000, comprised of the receipt of $2,000,000 from ARPA-H, offset by $113,000 of costs associated with the 2024 Direct Offering Agreement that was allocated to the modification of the August 2022 Warrants.

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***Cash Flows*** The following table summarizes our cash flows for the periods ended December 31, 2025 and 2024.

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| | | |
|:---|:---|:---|
| | **Year Ended**  | **Year Ended**  |
| | **December 31,**  | **December 31,**  |
| <br>**(in thousands)** | **2025** | **2024** |
| Net cash (used in) provided by: |  |  |
| &nbsp;&nbsp;Operating activities | $(7029) | $(12113) |
| &nbsp;&nbsp;Investing activities | (401) | (37) |
| &nbsp;&nbsp;Financing activities | 7416 | 11064 |
| Net decrease in cash and cash equivalents | $(14) | $(1086) |

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Net cash used in operating activities for the year ended December 31, 2025 resulted primarily from the net loss reported of $12,780,000, approximately $1,148,000 related to changes in accrued liabilities and $936,000 related to changes in accounts payable, primarily offset by $5,607,000 related to changes in fair value of warrant liabilities, $1,198,000 related to changes in fair value of convertible notes, $531,000 related to non-cash stock compensation expense and $412,000 related to financing expense for entering into an equity line of credit with Lincoln Park.

Net cash used in operating activities for the year ended December 31, 2024 resulted primarily from the net loss reported of $13,094,000 and changes in fair value of warrant liabilities in the amount of approximately $1,346,000 and $418,000 related to changes in accrued liabilities, primarily offset by $1,494,000 related to non-cash stock compensation expense, $912,000 related to changes in accounts payable and $469,000 related to changes in accounts receivable.

Net cash used in investing activities consisted primarily of intangible assets and property and equipment purchases for the year ended December 31, 2025 and property and equipment purchases for the year ended December 31, 2024.

Net cash provided by financing activities for the year ended December 31, 2025 related primarily to net proceeds from an at the market offering resulting in net proceeds of $3,337,000, after deducting placement agent costs and other expenses of $147,000, net proceeds of $2,809,000 related to a private placement offering, after deducing offering costs of $140,000, net proceeds of $1,366,000 related to convertible notes and proceeds from a warrant exercise of $112,000, partially offset by principal payments on the DECD loan of $233,000.

Net cash provided by financing activities for the year ended December 31, 2024 related primarily to a registered direct offering resulting in net proceeds of $4,830,000, after deducting placement agent costs and other expenses of $733,000, net proceeds of $1,901,000 related to an equity line of credit agreement, net proceeds of $1,838,000 related to a private placement offering, after deducting placement agent costs and other expenses of $72,000, net proceeds of $1,862,000 related to a warrant inducement agreement, after deducting placement agent costs and other expenses of $277,000 and net proceeds of $715,000 related to an at the market offering, after deducting transaction-related offering costs of $189,000, partially offset by principal payments on the DECD loan of $93,000.

We have significant NOL carryforwards as of December 31, 2025 which are subject to a full valuation allowance due to our history of operating losses. Section 382 of the Internal Revenue Code of 1986, as amended ("Section 382"), as well as similar state provisions restrict our ability to use our NOL credit carryforwards to offset taxable income due to ownership change limitations that have occurred in the past or that could occur in the future. These ownership changes also may limit the amount of tax credit carryforwards that can be utilized annually to offset tax liabilities.

Our pre- 2018 federal NOLs will expire in varying amounts from 2025 through 2037, if not utilized; and can offset 100% of future taxable income for regular tax purposes. Any federal NOLs arising on or after January 1, 2018, can be carried forward indefinitely but such federal NOL carryforwards are permitted to be used in any taxable year to offset up to 80% of taxable income in such year. Portions of our state NOLs will expire in varying amounts from 2025 through 2044 if not utilized. Our ability to use our NOLs will be dependent on our ability to generate taxable income, and the portions of our NOLs could expire before we generate sufficient taxable income.

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Our ability to use our NOL carryforwards to offset taxable income is restricted due to ownership change limitations that have occurred in the past or that could occur in the future, as required by Section 382, as well as similar state specific provisions.

Our management believes that Section 382 ownership changes most recently occurred as a result of our follow-on public offerings in 2011 and 2013.

These limitations may result in the expiration of a portion of our NOL carryforwards before utilization. Due to the existence of a full valuation allowance against our remaining NOLs, it is not expected that Section 382 limitations will have an impact on our results of operations or financial position. For additional information, see Note 11 to our consolidated financial statements, *Income Taxes*.

#### Liquidity and Capital Resources
We plan to continue to expend resources selling and marketing our ovarian cancer and endometriosis offerings and developing our pipeline and service capabilities.

We do not believe our existing cash and cash equivalents balance and cash flow from operations will be sufficient to meet our working capital, capital expenditures, and material cash requirements from known contractual obligations for the next twelve months and beyond. Our future capital requirements, the adequacy of available funds, and cash flows from operations could be affected by various risks and uncertainties, including, but not limited to, those detailed in Part I, Item 1A, Risk Factors in this Annual Report. We have incurred significant net losses and negative cash flows from operations since inception, and as a result has an accumulated deficit of approximately $544,177,000 as of December 31, 2025. We also expect to incur a net loss and negative cash flows from operations for 2026. In order to continue our operations as currently planned through 2026 and beyond, we will need to raise additional capital, which may include public or private equity offerings, debt financing, collaborations, licensing arrangements. Given the above conditions, there is substantial doubt about our ability to continue as a going concern. The consolidated financial statements have been prepared on a going concern basis and do not include any adjustments that might result from these uncertainties.

#### Contractual Obligations
*Loan Agreement*

In March 2016, we entered into a loan agreement (as amended on March 7, 2018 and April 3, 2020, the "DECD Loan Agreement") with the State of Connecticut Department of Economic and Community Development (the "DECD"), pursuant to which we may borrow up to $4,000,000 from the DECD.

Under the terms of the DECD Loan Agreement, if we had failed to maintain our Connecticut operations through March 22, 2026, the DECD may have required early repayment of a portion or all of the loan plus a penalty of 5% of the total funded loan. For additional information, see Note 7 to our consolidated financial statements, *Commitments, Contingencies and Debt*. If we choose to repay the loan early, it may be done at any time without premium or penalty. As of December 31, 2025, the remaining balance outstanding under the DECD Loan Agreement is approximately $1,277,000, net of issuance costs.

*Operating Leases*

As of December 31, 2025, we are engaged in two lease agreements. Our Austin, Texas lease renewal agreement has a term of 81 months and expires on August 31, 2031, with the option to extend the lease for an additional three years. Our Shelton, Connecticut lease renewal agreement has a five-year term and expires on September 30, 2028, with a five-year renewal option.

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*Non-cancelable Royalty Obligations and Other Commitments*

We are a party to an amended research collaboration agreement with The Johns Hopkins University School of Medicine under which we license certain of its intellectual property directed at the discovery and validation of biomarkers in human subjects, including but not limited to clinical application of biomarkers in the understanding, diagnosis and management of human disease. Under the terms of the amended research collaboration agreement, Aspira is required to pay the greater of 4% royalties on net sales of diagnostic tests using the assigned patents or annual minimum royalties of $57,500. Royalty expense for the years ended December 31, 2025 and 2024 totaled $289,000 and $293,000, respectively, as recorded in cost of revenue in the consolidated statements of operations.

*Business Agreements*

In August 2022, we entered into a sponsored research agreement with Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz (the "Dana-Farber, Brigham, Lodz Research Agreement") for the generation of a multi-omic, non-invasive diagnostic aid to identify endometriosis based on circulating miRNAs and proteins. The "Dana-Farber, Brigham, Lodz Research Agreement" requires payments to be made upon the achievement of certain milestones. Under the terms of and as further described in the agreement, payments of approximately $1,252,000 were made by us to the counterparties upon successful completion of certain deliverables as follows: 68% was paid in 2022, 15% was paid in 2023 and the remaining 17% was paid in 2025. During the year ended December 31, 2025, approximately $50,000 has been recorded as research and development expense in our consolidated financial statement of operations for the project. During the year ended December 31, 2024, approximately $118,000, was recorded as research and development expense in our consolidated financial statement of operations for the project. From the inception of the Dana-Faber, Brigham, Lodz Research Agreement through December 31, 2025, research and development expenses in the cumulative amount of $1,252,000 have been recorded and paid.

On March 20, 2023, we entered into a licensing agreement with Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz (the "Ovarian Cancer License Agreement") under which the Company will license certain of its intellectual property to be used in our OvaSuite product portfolio. Under the terms of the Ovarian Cancer License Agreement, we paid an initial license fee of $75,000 and pay an annual license maintenance fee of $50,000 on each anniversary of the date, as well as non-refundable royalty payments of up to $1,350,000 based on certain regulatory approvals and commercialization milestones and further royalty payments based on the net sales of our products included. No milestones have been reached as of December 31, 2025, and no royalty payments have been paid to date.

#### Common Stock
On March 28, 2023, we entered into a purchase agreement (the "2023 Equity Line of Credit Agreement") with Lincoln Park Capital Fund, LLC ("Lincoln Park") and a registration rights agreement (the "LPC Registration Rights Agreement"), pursuant to which we had the right, in our sole discretion, to sell to Lincoln Park shares of our common stock, par value $0.001 per share (the "Common Stock"), having an aggregate value of up to $10,000,000 (the "Purchase Shares"), subject to certain limitations and conditions set forth in the 2023 Equity Line of Credit Agreement.

The issuance of the Purchase Shares had been previously registered pursuant to our effective shelf registration statement on Form S-3 (File No. 333-252267) (the "Old Registration Statement"), and the related base prospectus included in the Registration Statement, as supplemented by a prospectus supplement filed on March 28, 2023, that has expired. On April 22, 2024, we filed a registration statement on Form S-3 (File No. 333-278867) (the "Registration Statement"), and the related base prospectus included in the Registration Statement, that was declared effective by the SEC on April 25, 2024.

During the year ended December 31, 2024, we sold 949,574 shares under the 2023 Equity Line of Credit Agreement for gross proceeds of approximately $1,900,000. Over the life of the 2023 Equity Line of Credit Agreement through December 31, 2025, we sold 1,310,517 shares for gross proceeds of approximately $3,078,000. We incurred approximately $326,000 of costs related to the execution of the 2023 Equity Line of Credit Agreement, all of which are reflected in the consolidated financial statements. Of the total costs incurred, approximately $258,000 was paid in common

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stock to Lincoln Park for a commitment fee and $30,000 was paid for Lincoln Park expenses. These transaction costs were included in other expense in our consolidated statement of operations for the year ended December 31, 2023. We incurred approximately $41,000 and $0 for legal fees during the year ended December 31, 2025 and 2024, respectively, and included the costs in general and administrative expenses on its consolidated statement of operations. Under the terms of the Warrant Inducement Agreement, we agreed not to sell shares under the 2023 Equity Line of Credit Agreement for six months from the effective date of the Form S-3, which was September 3, 2024. As of March 30, 2026, the remaining availability under the 2023 Equity Line of Credit Agreement was $1,700,000 of shares of Common Stock that can be sold to Lincoln Park under the 2023 Equity Line of Credit Agreement; however, as we are no longer traded on Nasdaq, we do not have access to this facility.

On January 24, 2024, we entered into a securities purchase agreement (the "2024 Direct Offering Agreement"), with several investors relating to the issuance and sale of 1,371,000 shares of our common stock, par value $0.001 per share, and pre-funded warrants to purchase 200,000 shares of Common Stock (the "Pre-Funded Warrants"), in a registered direct offering, together with accompanying warrants to purchase 1,571,000 shares of Common Stock (the "Purchase Warrants", and together with the Pre-Funded Warrants, the "Warrants") in a concurrent private placement (the "Concurrent Private Offering" and together with the registered direct offering, the "2024 Direct Offering").

Pursuant to the 2024 Direct Offering Agreement, we issued 1,368,600 shares of common stock to certain investors at an offering price of $3.50 per share, and 2,400 shares of common stock to an executive officer, at an offering price of $4.255 per share, which was the consolidated closing bid price of our common stock on The Nasdaq Capital Market on January 24, 2024 of $4.13 per share plus $0.125 per Purchase Warrant. The purchase price of each Pre-Funded Warrant was equal to the combined purchase price at which a share of Common Stock and the accompanying Purchase Warrant is sold in this 2024 Direct Offering, minus $0.0001. Our gross proceeds from the 2024 Direct Offering were approximately $5,563,000, before deducting placement agent fees and other expenses of approximately $733,000 payable by us. The 2024 Direct Offering closed on January 26, 2024.

All of the Pre-Funded Warrants were exercised on February 6, 2024.

The Purchase Warrants have an exercise price of $4.13 per share and were exercisable beginning six months after issuance. 1,400,000 of the Purchase Warrants were exercised on August 1, 2024 under the Warrant Inducement Agreement at a reduced price of $1.25 per share.

We engaged AGP to act as sole placement agent in the 2024 Direct Offering. We paid the placement agent a cash fee equal to 7.0% of the aggregate gross proceeds generated from the 2024 Direct Offering, except that, with respect to proceeds raised in this 2024 Direct Offering from certain designated persons, AGP's cash fee is reduced to 3.5% of such proceeds, and to reimburse certain fees and expenses of the placement agent in connection with the 2024 Direct Offering. We also reimbursed the placement agent for its accountable offering-related legal expenses of $75,000 and a non-accountable expense allowance of $30,000. Costs related to the 2024 Direct Offering were recorded as an offset to additional paid-in capital on our balance sheet as of December 31, 2024.

Effective upon the closing of the 2024 Direct Offering, we also amended certain existing warrants (the "August 2022 Warrants") to purchase up to an aggregate of 366,664 shares at an exercise price of $13.20 per share and a termination date of August 25, 2027, so that the amended August 2022 Warrants have a reduced exercise price of $4.13 per share and a new termination date of January 26, 2029. The other terms of the amended August 2022 Warrants remain unchanged. We performed an analysis of the fair value of the August 2022 Warrants immediately before and after the modification and the increase in fair value of the August 2022 Warrants of $490,000 was recorded as a change in fair value of warrant liabilities in our consolidated statement of operations.

Approximately $106,000 of the costs related to the 2024 Direct Offering were allocated to the August 2022 Warrants and were recorded as other expense in our consolidated statement of operations.

On July 1, 2024, we entered into a securities purchase agreement with certain investors in a private placement (the "2024 Private Placement Offering"). Pursuant to the 2024 Private Placement Offering, we issued an aggregate of 1,248,529 shares of our common stock and accompanying warrants (the "July 2024 Warrants") to purchase an equal

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number of shares of common stock at a price of $1.53 per share and accompanying warrant. The July 2024 Warrants have an exercise price of $2.25 per share and are exercisable until their expiration on the third anniversary of the issuance date. Our gross proceeds from the 2024 Private Placement Offering were approximately $1,909,000, before deducting expenses of approximately $72,000 payable by us.

In February 2025, certain July 2024 Warrants were modified to require shareholder approval of the July 2024 Warrants prior to their becoming exercisable.

On July 31, 2024, we entered into a warrant inducement agreement (the "Warrant Inducement Agreement") with a certain holder (the "Holder") of (i) warrants to purchase 311,111 shares of Common Stock dated August 22, 2022 (the "August 2022 Warrants") and (ii) warrants to purchase 1,400,000 shares of Common Stock dated January 26, 2024 (the "January 2024 Warrants"), pursuant to which the Holder agreed to exercise in cash the warrants held at a reduced exercise price of $1.25 per share (reduced from $4.13 per share for the August 2022 Warrants and $4.13 for the January 2024 Warrants).

As an inducement to such exercise, we agreed to issue to the Holder new Common Stock warrants (collectively, the "August 2024 Warrants"), to purchase up to 2,566,667 shares of Common Stock. The August 2024 Warrants were exercisable immediately after issuance and will expire 5 years from the initial exercise date.

The transaction, which closed on August 1, 2024, resulted in net proceeds of approximately $1,862,000. The Warrant Inducement Agreement was entered into to encourage the exercise of the August 2022 Warrants and January 2024 Warrants in order to obtain capital for operations. The $1,323,000 incremental value transferred for the modification to both the August 2022 Warrants and January 2024 Warrants as a result of the Warrant Inducement Amendment was accounted for as an equity issuance cost and recognized within additional paid in capital in the audited consolidated balance sheets.

On August 2, 2024, we entered into an agreement with H.C. Wainwright in connection with an At the Market offering agreement (the "2024 At the Market Offering") to sell shares of our common stock ("Common Stock"), having an aggregate sales price of up to $4,450,000, from time to time, through an "at the market offering" program under which H.C. Wainwright acts as sales agent. We pay Wainwright a commission rate equal to 3.0% of the aggregate gross proceeds from each sale of shares under the 2024 At the Market Offering. We have also reimbursed H.C. Wainwright for certain specified expenses in connection with entering into the 2024 At the Market Offering.

During the year ended December 31, 2025, we sold 12,277,441 shares under the 2024 At the Market Offering Agreement for gross proceeds of approximately $3,483,000 before deducting expenses of approximately $146,000. During the year ended December 31, 2024, we sold 1,073,050 shares under the 2024 At the Market Offering for gross proceeds of approximately $903,000. We incurred approximately $240,000 of costs related to the execution of the 2024 At the Market Offering, all of which were recorded as an offset to additional paid-in capital on our balance sheet as of December 31, 2024.

On March 12, 2025, we issued warrants (the "March 2025 Warrants") upon the conversion of the Convertible Notes (see Note 7 to our consolidated financial statements, *Commitments, Contingencies and Debt*). The March 2025 Warrants were exercisable for five years at $0.25 per share for the first 24 months after issuance, and $0.50 per share thereafter. In September 2025, the March 2025 Warrants were modified to a single exercise price of $0.35 per share and the termination date was extended to March 5, 2031.

On April 4, 2025, we entered into an equity purchase agreement (the "2025 Equity Purchase Agreement") with Triton Funds L.P. ("Triton") for the purchase of up to $2.0 million of our shares of common stock.

Pursuant to the 2025 Equity Purchase Agreement, the Company issued 354,988 shares of restricted common stock to Triton for $25,000 and upon effectiveness of a registration statement registering the shares of Common Stock, we would be able to close on the sale of up to $2 million of Common Stock. Transaction costs were approximately $214,000. The 2025 Equity Purchase Agreement expired on September 30, 2025.

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On April 15, 2025, the Company was delisted from the trading of its Common Stock on the Nasdaq Stock Market. As a result of the delisting, the 2024 At the Market Offering Agreement was terminated.

On September 16, 2025, we entered into a securities purchase agreement with certain investors and board members in a private placement (the "2025 Private Placement Offering"). Pursuant to the 2025 Private Placement Offering, we issued an aggregate of 6,550,000 shares of our common stock and accompanying warrants (the "September 2025 Warrants") to purchase an equal number of shares of common stock at a price of $0.45 per share and accompanying warrant. The September 2025 Warrants have an exercise price of $0.75 per share and are exercisable until their expiration on the fifth anniversary of the issuance date. The gross proceeds from the 2025 Private Placement Offering were approximately $2,949,000, before deducting issuance costs of approximately $140,000.

On December 23, 2025, we entered into an equity purchase agreement (the "2025 Lincoln Park Agreement") with Lincoln Park Capital Fund, LLC ("Lincoln Park"), for the purchase of up to an aggregate of $10.0 million of our Common Stock.

Pursuant to the 2025 Lincoln Park Agreement, on any business day on which the closing sale price of the Common Stock is greater than $0.10 per share, we may direct Lincoln Park to purchase up to 50,000 shares of Common Stock (a "Regular Purchase"), which amount may be increased to up to 75,000 shares if the closing sale price is not below $0.50 per share and up to 100,000 shares if the closing sale price is not below $0.75 per share, in each case subject to a maximum dollar amount of $500,000 per Regular Purchase. The purchase price per share for each Regular Purchase will be equal to 95% of the lower of (i) the lowest sale price of the Common Stock on the applicable purchase date and (ii) the average of the three lowest closing sale prices of the Common Stock during the ten business days immediately preceding the applicable purchase date. Regular Purchases may be effected as frequently as each business day after the close of trading so that the applicable purchase price is fixed and known at the time we elect to sell shares to Lincoln Park.

In addition, if we direct Lincoln Park to purchase the maximum number of shares permitted in a Regular Purchase on an applicable purchase date, then, in addition to such Regular Purchase and subject to the satisfaction of certain conditions and limitations set forth in the Purchase Agreement, we may also direct Lincoln Park to purchase additional shares of Common Stock in one or more accelerated purchases (each, an "Accelerated Purchase") on the following business day. We may set a minimum price threshold for any Accelerated Purchase in the related notice. For any Accelerated Purchase, Lincoln Park will purchase the lesser of (i) three times the number of shares purchased in the corresponding Regular Purchase and (ii) 30% of the trading volume on the Accelerated Purchase date, at a purchase price per share equal to the lower of 95% of (x) the closing sale price on the Accelerated Purchase date and (y) the volume-weighted average price for such date. Subject to satisfaction of the applicable conditions, we may direct multiple Accelerated Purchases in a single trading day, provided share deliveries for prior purchases have been completed.

We have incurred significant net losses and negative cash flows from operations since inception. At December 31, 2025 we had an accumulated deficit of $544,177,000 and stockholders' deficit of $6,932,000. As of December 31, 2025, we had $1,755,000 of cash and cash equivalents, and $3,465,000 of current liabilities. Our working capital was $598,000 at December 31, 2025. There can be no assurance that we will achieve or sustain profitability or positive cash flow from operations. In addition, while we expect to grow revenue through Aspira Labs, there is no assurance of our ability to generate substantial revenues and cash flows from Aspira Labs' operations. We expect revenue from our products to be our only material, recurring source of cash in 2026.

We expect to incur a net loss and negative cash flows from operations in 2026.

Our future liquidity and capital requirements will depend upon many factors, including, among others:

● resources devoted to sales, marketing and distribution capabilities;

● the rate of product adoption by physicians and patients;

● the rate of product adoption by healthcare systems and large physician practices of the decentralized distribution agreements;

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● the insurance payer community's acceptance of and reimbursement for our products;

● our plans to acquire or invest in other products, technologies and businesses; and

● the potential need to add study sites to access additional patients to maintain clinical timelines;

In the event that our existing cash on hand is not sufficient to fund our near or long term operations, meet our capital requirements or satisfy our anticipated obligations as they become due, we expect to take further action to protect our liquidity position. Such actions may include, but are not limited to:

● raising capital through an equity offering either in the public markets or via a private placement offering (however, no assurance can be given that capital will be available on acceptable terms, or at all);

● reducing or eliminating executive bonuses or replacing cash compensation with equity grants;

● reducing professional services and consulting fees and eliminating non-critical projects;

● reducing travel and entertainment expenses; and

● reducing, eliminating or deferring discretionary marketing programs.

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|:---|:---|
| **ITEM 7A.** | **QUANTITATIVE AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK** |

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Pursuant to Item 305(e) of Regulation S-K, the information called for by Item 7A is not required.

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|:---|:---|
| **ITEM 8.** | **CONSOLIDATED FINANCIAL STATEMENTS AND SUPPLEMENTARY DATA** |

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Our consolidated financial statements, including consolidated balance sheets as of December 31, 2025 and 2024, consolidated statements of operations for the years ended December 31, 2025 and 2024, consolidated statements of changes in stockholders' equity for the years ended December 31, 2025 and 2024, consolidated statements of cash flows for the years ended December 31, 2025 and 2024 and notes to our consolidated financial statements, together with a report thereon of our independent registered public accounting firm are attached hereto as pages F-1 through F-34.

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|:---|:---|
| **ITEM 9.** | **CHANGES IN AND DISAGREEMENTS WITH ACCOUNTANTS ON ACCOUNTING AND FINANCIAL DISCLOSURE** |

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None.

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|:---|:---|
| **ITEM 9A.** | **CONTROLS AND PROCEDURES** |

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#### Evaluation of Disclosure Controls and Procedures
Our senior management is responsible for establishing and maintaining a system of disclosure controls and procedures (as defined in Rule 13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934, as amended (the "Exchange Act") designed to ensure that information required to be disclosed by us in the reports that we file or submit under the Exchange Act is recorded, processed, summarized and reported within the time periods specified in the SEC's rules and forms. Disclosure controls and procedures include, without limitation, controls and procedures designed to ensure that information required to be disclosed by an issuer in the reports that it files or submits under the Exchange Act is accumulated and communicated to the issuer's management, including its principal executive officer and principal financial officer, or persons performing similar functions, as appropriate to allow timely decisions regarding required disclosure.

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Management, including our Chief Executive Officer and our Chief Financial Officer, performed an evaluation of our disclosure controls and procedures (as defined in Rules 13a-15(e) and 15d-15(e) under the Exchange Act) as of December 31, 2025. Based on this evaluation, our Chief Executive Officer and Chief Financial Officer have concluded that as of December 31, 2025, our disclosure controls and procedures were effective.

#### Management's Annual Report on Internal Control over Financial Reporting
We are responsible for establishing and maintaining adequate internal control over our financial reporting, as such term is defined in Exchange Act Rules 13a-15(f) and 15d-15(f). Our management has assessed the effectiveness of internal control over financial reporting as of December 31, 2025. Our assessment was based on criteria set forth by the Committee of Sponsoring Organizations of the Treadway Commission ("COSO") entitled "Internal Control - Integrated Framework (2013)."

Our internal control over financial reporting is a process designed to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for external purposes in accordance with GAAP. Our internal control over financial reporting includes those policies and procedures that:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(i) pertain to the maintenance of records that, in reasonable detail, accurately and fairly reflect our transactions and dispositions of our assets;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(ii) provide reasonable assurance that transactions are recorded as necessary to permit preparation of financial statements in accordance with GAAP, and that our receipts and expenditures are being made only in accordance with authorizations of our management and board of directors; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(iii) provide reasonable assurance regarding prevention or timely detection of unauthorized acquisition, use, or disposition of our assets that could have a material effect on the financial statements.

Because of its inherent limitations, internal control over financial reporting may not prevent or detect misstatements. Also, projections of any evaluation of effectiveness to future periods are subject to the risk that controls may become inadequate because of changes in conditions, or that the degree of compliance with the policies or procedures may deteriorate.

Based on using the COSO criteria, management concluded our internal control over financial reporting as of December 31, 2025 was effective.

This Form 10-K does not include an attestation report of our independent registered public accounting firm regarding internal control over financial reporting. Management's assessment of the effectiveness of our internal control over financial reporting as of December 31, 2025 was not subject to attestation by our independent registered public accounting firm pursuant to rules of the SEC that permit a smaller reporting company to provide only management's report in our Annual Report on Form 10-K.

#### Limitations on Effectiveness of Controls and Procedures and Internal Control over Financial Reporting
In designing and evaluating the disclosure controls and procedures and internal control over financial reporting, management recognizes that any controls and procedures, no matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives. In addition, the design of disclosure controls and procedures and internal control over financial reporting must reflect the fact that there are resource constraints, and that management is required to apply judgment in evaluating the benefits of possible controls and procedures relative to their costs.

#### Remediation of Previously Identified Material Weaknesses
Management previously identified a material weakness in our internal control over financial reporting as of December 31, 2024 related to the operation of internal controls related to our contract review processes and the accounting

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for such contracts. This material weakness related to the accounting for complex financial transactions, including the technical accounting conclusions reached. During the year ended December 31, 2024, we entered into two transactions that were considered to be significant, non-routine or complex transactions, including a warrant inducement and a government grant. While the control was adequately designed, the operation of the control was not effective for either transaction.

As of December 31, 2025, management implemented the following to address the previously identified material weakness related to the contract review process:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;● Enhanced internal controls around the identification of material contracts

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;● Enhanced internal controls around technical memos relating to contracts

Management determined these controls were in place and operating for a sufficient period of time as of December 31, 2025 and, therefore, the previously identified material weakness related to complex financial transactions was remediated as of December 31, 2025.

Changes in Internal Control over Financial Reporting

Other than as described above, there were no changes in our internal control over financial reporting during the quarter ended December 31, 2025 that materially affected, or are reasonably likely to materially affect, our internal control over financial reporting.

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|:---|:---|
| **ITEM 9B.** | **OTHER INFORMATION** |

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None.

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|:---|:---|
| **ITEM 9C.** | **DISCLOSURE REGARDING FOREIGN JURISDICTIONS THAT PREVENT INSPECTIONS** |

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Not applicable.

#### PART III

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|:---|:---|
| **ITEM 10.** | **DIRECTORS, EXECUTIVE OFFICERS AND CORPORATE GOVERNANCE** |

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The information regarding our directors, committees of our board of directors, our director nomination process, and our executive officers appearing under the heading "Election of Directors," "Corporate Governance," "Management", "Security Ownership of Certain Beneficial Owners and Management" and "Delinquent Section 16(a) Reports" of the Company's proxy statement relating to our annual meeting of stockholders to be held in 2026 (the "2026 Proxy Statement") is incorporated by reference.

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|:---|:---|
| **ITEM 11.** | **EXECUTIVE COMPENSATION** |

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The information appearing under the headings "Board Compensation," and "Executive Officer Compensation," of the 2026 Proxy Statement is incorporated by reference.

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|:---|:---|
| **ITEM 12.** | **SECURITY OWNERSHIP OF CERTAIN BENEFICIAL OWNERS AND MANAGEMENT AND RELATED STOCKHOLDER MATTERS** |

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The information appearing under the heading "Security Ownership of Certain Beneficial Owners and Management" of the 2026 Proxy Statement is incorporated by reference.

The information required by Item 201(d) of Regulation S-K will be set forth in the section titled "Equity Compensation Plan Information" in the 2026 Proxy Statement and is incorporated herein by reference.

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|:---|:---|
| **ITEM 13.** | **CERTAIN RELATIONSHIPS AND RELATED TRANSACTIONS, AND DIRECTOR INDEPENDENCE** |

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The information appearing under the headings "Certain Relationships and Related Transactions" and "Corporate Governance" of the 2026 Proxy Statement is incorporated by reference.

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|:---|:---|
| **ITEM 14.** | **PRINCIPAL ACCOUNTANT FEES AND SERVICES** |

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The information appearing under the heading "Ratification of the Selection of the Independent Registered Public Accounting Firm" of the 2026 Proxy Statement is incorporated by reference.

#### PART IV

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| | |
|:---|:---|
| **ITEM 15.** | **EXHIBITS, FINANCIAL STATEMENT SCHEDULES** |

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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) LIST OF DOCUMENTS FILED AS PART OF THIS REPORT:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;*1.* *Financial Statements* 

The financial statements and notes thereto, and the report of the independent registered public accounting firm thereon, are set forth on pages F-1 through F-34.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;*2.* *Financial Statement Schedules* 

All financial statement schedules have been omitted as the information is not required under the related instructions or is not applicable or because the information required is already included in the financial statements or the notes to those financial statements.

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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) EXHIBITS

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| | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|
| Exhibit |  | Incorporated by Reference | Incorporated by Reference | Incorporated by Reference | Incorporated by Reference | Filed |
| Number | Exhibit Description | Form | File No. | Exhibit | Filing Date | Herewith |
| 3.1 | [Fourth Amended and Restated Certificate of Incorporation of Aspira Women's Health Inc. dated January 22, 2010](https://www.sec.gov/Archives/edgar/data/926617/000095012310004797/f54711exv3w1.htm) | 8-K | 000-31617 | 3.1 | January 25, 2010 |  |
| 3.2 | [Certificate of Amendment of Fourth Amended and Restated Certificate of Incorporation, effective June 19, 2014](https://www.sec.gov/Archives/edgar/data/926617/000092661714000044/c617-20140630ex32e1c2a07.htm) | 10-Q | 001-34810 | 3.2 | August 14, 2014 |  |
| 3.3 | [Certificate of Amendment to Fourth Amended and Restated Certificate of Incorporation of Vermillion, Inc. dated June 11, 2020](https://www.sec.gov/Archives/edgar/data/926617/000092661720000046/vrml-20200611xex3_1.htm) | 8-K | 001-34810 | 3.1 | June 11, 2020 |  |
| 3.4 | [Certificate of Amendment to Fourth Amended and Restated Certificate of Incorporation of Aspira Women's Health Inc, dated February 7, 2023](https://www.sec.gov/Archives/edgar/data/926617/000092661723000011/awh-20230206xex3_1.htm) | 8-K | 001-34810 | 3.1 | February 7, 2023 |  |
| 3.5 | [Amended and Restated Bylaws of Aspira Women's Health Inc., effective March 28, 2025](https://www.sec.gov/Archives/edgar/data/926617/000164117225001708/ex3-1.htm) | 8-K | 001-34810 | 3.1 | March 31, 2025 |  |
| 3.6 | [Certificate of Amendment to the Fourth Amended and Restated Certificate of Incorporation of Aspira Women's Health Inc., as amended May 11, 2023](https://www.sec.gov/Archives/edgar/data/926617/000092661723000042/awh-20230511xex3_1.htm) | 8-K | 001-34810 | 3.1 | May 11, 2023 |  |
| 4.1 | [Form of Aspira Women's Health Inc.'s (formerly Ciphergen Biosystems, Inc.) Common Stock Certificate](https://www.sec.gov/Archives/edgar/data/926617/000091205700039015/ex-4_1.txt) | S-1/A | 333-32812 | 4.1 | August 24, 2000 |  |
| 4.2 | [Securities Purchase Agreement dated May 8, 2013, by and among Aspira Women's Health Inc. (formerly Vermillion, Inc.) and the purchasers identified therein](https://www.sec.gov/Archives/edgar/data/926617/000119312513219609/d537389dex101.htm) | 8-K | 001-34810 | 10.1 | May 14, 2013 |  |
| 4.3 | [Stockholders Agreement dated May 13, 2013, by and among Vermillion, Inc., Oracle Partners, LP, Oracle Ten Fund Master, LP, Jack W. Schuler and other purchasers named therein](https://www.sec.gov/Archives/edgar/data/926617/000119312513219609/d537389dex102.htm) | 8-K | 001-34810 | 10.2 | May 14, 2013 |  |
| 4.4 | [Amended and Restated Promissory Note #1 by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective April 3, 2020](https://www.sec.gov/Archives/edgar/data/926617/000092661720000015/vrml-20191231xex4_4.htm) | 10-K | 001-34810 | 4.4 | April 7, 2020 |  |
| 4.5 | [Amended and Restated Promissory Note #2 by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective April 3, 2020](https://www.sec.gov/Archives/edgar/data/926617/000092661720000015/vrml-20191231xex4_5.htm) | 10-K | 001-34810 | 4.5 | April 7, 2020 |  |
| 4.6 | [Description of Aspira Women's Health Inc.'s Securities Pursuant to Section 12 of the Securities Exchange Act of 1934](https://www.sec.gov/Archives/edgar/data/926617/000092661724000015/awh-20231231xex4_7.htm) | 10-K | 001-34810 | 4.7 | April 1, 2024 |  |
| 4.7 | [Form of Warrant 2022](https://www.sec.gov/Archives/edgar/data/926617/000092661724000015/awh-20231231x10k.htm) | 8-K | 001-34810 | 4.1 | August 24, 2022 |  |
| 4.8 | [Form of Warrant Amendment to Common Stock Purchase Warrant_2022](https://www.sec.gov/Archives/edgar/data/926617/000119312524016864/d699303dex43.htm) | 8-K | 001-34810 | 4.3 | January 26, 2024 |  |
| 4.9 | [Form of Warrant 2024](https://www.sec.gov/Archives/edgar/data/926617/000119312524016864/d699303dex42.htm) | 8-K | 001-34810 | 4.2 | January 26, 2024 |  |
| 4.10 | [Form of Warrant June 2024](https://www.sec.gov/Archives/edgar/data/926617/000095017024080040/awh-ex4_1.htm) | 8-K | 001-34810 | 4.1 | July 2, 2024 |  |
| 4.11 | [Form of Warrant July 2024](https://www.sec.gov/Archives/edgar/data/926617/000095017024088246/awh-ex4_1.htm) | 8-K | 001-34810 | 4.1 | July 31, 2024 |  |
| 4.12 | [Form of Warrant Amendment to Common Stock Purchase Warrant June 2024](https://www.sec.gov/Archives/edgar/data/926617/000164117225000983/ex4-14.htm) | 10-K | 001-34810 | 4.14 | March 27, 2025 |  |
| 4.13 | [Form of Senior Convertible Note](https://www.sec.gov/Archives/edgar/data/926617/000149315225009784/ex4-1.htm) | 8-K | 001-34810 | 4.1 | March 11, 2025 |  |
| 4.14 | [Form of Warrant](https://www.sec.gov/Archives/edgar/data/926617/000149315225009784/ex4-2.htm) March 2025 | 8-K | 001-34810 | 4.2 | March 11, 2025 |  |
| 4.15 | [Form of Warrant September 2025](https://www.sec.gov/Archives/edgar/data/926617/000149315225014925/ex4-1.htm) | 8-K | 001-34810 | 4.1 | September 25, 2025 |  |
| 4.16 | [Form of Amended and Restated Warrant, issued September 19, 202](https://www.sec.gov/Archives/edgar/data/926617/000110465925105561/awhl-20250919xex4d1.htm)5 | 8-K/A | 001-34810 | 4.1 | November 3, 2025 |  |
| 10.1 | [Form of Aspira Women's Health Inc Stock Option Award Agreement](https://www.sec.gov/Archives/edgar/data/926617/000092661722000052/awh-20220630xex10_4.htm) # | 10-Q | 001-34810 | 10.4 | August 10, 2022 |  |
| 10.2 | [Form of Aspira Women's Health Inc Restricted Stock Award Agreement](https://www.sec.gov/Archives/edgar/data/926617/000092661722000052/awh-20220630xex10_5.htm) # | 10-Q | 001-34810 | 10.5 | August 10, 2022 |  |
| 10.3 | [Aspira Women's Health Inc. 2019 Stock Incentive Plan](https://www.sec.gov/Archives/edgar/data/926617/000092661722000052/awh-20220630xex10_3.htm) # | 10-Q | 001-34810 | 10.3 | August 10, 2022 |  |
| 10.4 | [Form of Aspira Women's Health Inc. Stock Option Award Agreement (non-employee)](https://www.sec.gov/Archives/edgar/data/926617/000092661722000052/awh-20220630xex10_7.htm) # | 10-Q | 001-34810 | 10.7 | August 10, 2022 |  |
| 10.5 | [Assistance Agreement by and between the State of Connecticut, acting by and through the Department of Economic and Community Development and Vermillion, Inc. effective March 22, 2016](https://www.sec.gov/Archives/edgar/data/926617/000092661716000090/c617-20160331xex10_1.htm) | 10-Q | 001-34810 | 10.1 | May 16, 2016 |  |

---

[**Table of Contents**](#TOC)

---

| | | | | | |
|:---|:---|:---|:---|:---|:---|
| 10.6 | [Patent Security Agreement by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective March 22, 2016](https://www.sec.gov/Archives/edgar/data/926617/000092661716000090/c617-20160331xex10_3.htm) | 10-Q | 001-34810 | 10.3 | May 16, 2016 |
| 10.7 | [Security Agreement by Vermillion, Inc. in favor of the State of Connecticut, acting by and through the Department of Economic and Community Development, effective March 22, 2016](https://www.sec.gov/Archives/edgar/data/926617/000092661716000090/c617-20160331xex10_4.htm) | 10-Q | 001-34810 | 10.4 | May 16, 2016 |
| 10.8 | [First Amendment to the Assistance Agreement by and between the State of Connecticut, acting by and through the Department of Economic and Community Development and Vermillion, Inc. dated March 7, 2018](https://www.sec.gov/Archives/edgar/data/926617/000092661718000012/vrml-20171231xex10_21.htm) | 10-K | 001-34810 | 10.21 | March 13, 2018 |
| 10.9 | [Second Amendment to the Assistance Agreement by and between the State of Connecticut, acting by and through the Department of Economic and Community Development and Vermillion, Inc. dated April 3, 2020](https://www.sec.gov/Archives/edgar/data/926617/000092661720000015/vrml-20191231xex10_22.htm) | 10-K | 001-34810 | 10.22 | April 7, 2020 |
| 10.10 | [License Agreement between Aspira Women's Health Inc. and Dana-Farber Cancer Institute, Inc. effective March 20, 2023](https://www.sec.gov/Archives/edgar/data/926617/000092661724000015/awh-20231231xex10_28.htm) | 10-K | 001-34810 | 10.28 | April 1, 2024 |
| 10.11 | [Form of Securities Purchase Agreement June 2024](https://www.sec.gov/Archives/edgar/data/926617/000095017024080040/awh-ex10_1.htm) | 8-K | 001-34810 | 10.1 | July 2, 2024 |
| 10.12 | [Form of Warrant Inducement Agreement June 2024](https://www.sec.gov/Archives/edgar/data/926617/000095017024088246/awh-ex10_1.htm) | 8-K | 001-34810 | 10.1 | July 31, 2024 |
| 10.13 | [Employment Agreement between Aspira Women's Health Inc. and Michael Buhle effective March 26, 2025](https://www.sec.gov/Archives/edgar/data/926617/000164117225001703/ex10-1.htm)# | 8-K | 001-34810 | 10.1 | March 31, 2025 |
| 10.14 | [Form of Securities Purchase Agreement](https://www.sec.gov/Archives/edgar/data/926617/000149315225009784/ex10-1.htm) | 8-K | 001-34810 | 10.1 | March 11, 2025 |
| 10.15 | [Equity Purchase Agreement dated April 4, 2025](https://www.sec.gov/Archives/edgar/data/926617/000164117225003135/ex10-1.htm) | 8-K | 001-34810 | 10.1 | April 8, 2025 |
| 10.16 | [Master Services Agreement between Aspira Women's Health Inc. and Brian Hungerford, dated September 2, 2025](https://www.sec.gov/Archives/edgar/data/926617/000164117225026441/ex10-1.htm) #† | 8-K | 001-34810 | 10.1 | September 3, 2025 |
| 10.17 | [Statement of Work by and between Aspira Women's Health Inc. and Brian Hungerford, dated September 2, 2025](https://www.sec.gov/Archives/edgar/data/926617/000164117225026441/ex10-2.htm) | 8-K | 001-34810 | 10.2 | September 3, 2025 |
| 10.18 | [Form of Securities Purchase Agreement, dated September 19, 2025](https://www.sec.gov/Archives/edgar/data/926617/000149315225013987/ex10-1.htm) | 8-K | 001-34810 | 10.1 | September 18, 2025 |
| 10.19 | [Form of Amendment to Securities Purchase Agreement, dated September 19, 2025](https://www.sec.gov/Archives/edgar/data/926617/000149315225014925/ex10-1.htm) | 8-K | 001-34810 | 10.1 | September 25, 2025 |
| 10.20 | [Laboratory Services Agreement between Aspira Women's Health Inc and Mayo Collaborative Services, Inc., dated December 16, 2025](https://www.sec.gov/Archives/edgar/data/926617/000092661726000012/awhl-20251223xex10d1.htm) | 8-K/A | 001-34810 | 10.1 | March 25, 2026 |
| 10.21 | [Purchase Agreement, dated December 23, 2025, by and between Aspira Women's Health Inc and Lincoln Park Capital Fund, LLC](https://www.sec.gov/Archives/edgar/data/926617/000110465925124245/awhl-20251223xex10d1.htm). | 8-K | 001-34810 | 10.1 | December 23, 2025 |
| 10.22 | [Registration Rights Agreement, dated December 23, 2025, by and between Aspira Women's Health Inc and Lincoln Park Capital Fund, LLC](https://www.sec.gov/Archives/edgar/data/926617/000110465925124245/awhl-20251223xex10d2.htm). | 8-K | 001-34810 | 10.2 | December 23, 2025 |
| 10.23 | [Subordinated Business Loan and Security Agreement, dated as of January 30, 2026.](https://www.sec.gov/Archives/edgar/data/926617/000092661726000007/awhl-20260130xex10d1.htm) | 8-K | 001-34810 | 10.1 | January 30, 2026 |
| 10.24 | [Form of Subordinated Secured Promissory Note](https://www.sec.gov/Archives/edgar/data/926617/000092661726000007/awhl-20260130xex10d1.htm)  | 8-K | 001-34810 | 10.2 | January 30, 2026 |
| 19.1 | [Aspira Women's Health Inc. Insider Trading Policy](https://www.sec.gov/Archives/edgar/data/926617/000164117225000983/ex19-1.htm) | 10-K | 001-34810 | 19.1 | March 27, 2025 |
| 21.0 | [Subsidiaries of Registrant](https://www.sec.gov/Archives/edgar/data/926617/000092661722000014/awh-20211231xex21_0.htm) | 10-K | 001-34810 | 21.0 | March 31, 2022 |
| 23.1 | [Consent of BDO USA, P.C., Independent Registered Public Accounting Firm](awhl-20251231xex23d1.htm) |  |  |  |  |
| 24.1 | [Power of Attorney (included on signature page hereto)](#POWEROFATTORNEY_411434) |  |  |  |  |
| 31.1 | [Certification of the Chief Executive Officer Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002](awhl-20251231xex31d1.htm) |  |  |  |  |
| 31.2 | [Certification of the Chief Financial Officer Pursuant to Section 302 of the Sarbanes-Oxley Act of 2002](awhl-20251231xex31d2.htm) |  |  |  |  |
| 32.1 | [Certification of the Chief Executive Officer and Chief Financial Officer pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002](awhl-20251231xex32d1.htm) |  |  |  |  |
| 97.1 | [Aspira Women's Health Incentive Compensation Recoupment Policy](https://www.sec.gov/Archives/edgar/data/926617/000092661724000015/awh-20231231xex97_1.htm) | 10-K | 001-34810 | 97.1 | March 29, 2024 |
| 101 | Interactive Data Files pursuant to Rule 405 of Regulation S-T formatted in Inline Extensible Business Reporting Language ("Inline XBRL") |  |  |  |  |
| 104 | Cover Page Interactive Data File (embedded within the Inline XBRL document) |  |  |  |  |

---

&nbsp;&nbsp;&nbsp;&nbsp;**√** Filed herewith

# Management contract or compensatory plan or arrangement.

[**Table of Contents**](#TOC)

&nbsp;&nbsp;&nbsp;&nbsp;† Confidential treatment has been granted with respect to certain provisions of this agreement. Omitted portions have been filed separately with the SEC.

---

| | |
|:---|:---|
| **ITEM 16.** | **FORM 10-K SUMMARY** |

---

None.

[**Table of Contents**](#TOC)

#### ASPIRA WOMEN'S HEALTH INC.

#### INDEX TO CONSOLIDATED FINANCIAL STATEMENTS

---

| | |
|:---|:---|
|  | **PageNo.** |
| [Report of Independent Registered Public Accounting Firm](#ReportofIndependentRegisteredPublicAccou) (BDO USA, P.C.; Boston, Massachusetts; PCAOB ID 243) | F-1 |
| [Consolidated Balance Sheets as of December 31, 2025 and 2024](#ConsolidatedBalanceSheets_863692) | F-3 |
| [Consolidated Statements of Operations for the years ended December 31, 2025 and 2024](#ConsolidatedStatementsofOperations_25449) | F-4 |
| [Consolidated Statements of Changes in Stockholders' Deficit for the years ended December 31, 2025 and 2024](#ConsolidatedStatementsofChangesinStockho) | F-5 |
| [Consolidated Statements of Cash Flows for the years ended December 31, 2025 and 2024](#ConsolidatedStatementsofCashFlows_356756) | F-6 |
| [Notes to Consolidated Financial Statements](#NotestoConsolidatedFinancialStatements_9) | F-7 |

---

[**Table of Contents**](#TOC)

**Report of Independent Registered Public Accounting Firm**

Shareholders and Board of Directors

Aspira Women's Health Inc.

Austin, TX

**Opinion on the Consolidated Financial Statements** 

We have audited the accompanying consolidated balance sheets of Aspira Women's Health Inc. (the "Company") as of December 31, 2025 and 2024, the related consolidated statements of operations, changes in stockholders' deficit, and cash flows for each of the years then ended, and the related notes (collectively referred to as the "consolidated financial statements"). In our opinion, the consolidated financial statements present fairly, in all material respects, the financial position of the Company at December 31, 2025 and 2024, and the results of its operations and its cash flows for the years then ended**,** in conformity with accounting principles generally accepted in the United States of America.

**Going Concern Uncertainty** 

The accompanying consolidated financial statements have been prepared assuming that the Company will continue as a going concern. As discussed in Note 1 to the consolidated financial statements, the Company has suffered recurring losses from operations and expects to continue to incur substantial losses in the future, which raise substantial doubt about its ability to continue as a going concern. Management's plans in regard to these matters are also described in Note 1. The consolidated financial statements do not include any adjustments that might result from the outcome of this uncertainty.

**Basis for Opinion**

These consolidated financial statements are the responsibility of the Company's management. Our responsibility is to express an opinion on the Company's consolidated financial statements based on our audits. We are a public accounting firm registered with the Public Company Accounting Oversight Board (United States) ("PCAOB") and are required to be independent with respect to the Company in accordance with the U.S. federal securities laws and the applicable rules and regulations of the Securities and Exchange Commission and the PCAOB.

We conducted our audits in accordance with the standards of the PCAOB. Those standards require that we plan and perform the audit to obtain reasonable assurance about whether the consolidated financial statements are free of material misstatement, whether due to error or fraud. The Company is not required to have, nor were we engaged to perform, an audit of its internal control over financial reporting. As part of our audits we are required to obtain an understanding of internal control over financial reporting but not for the purpose of expressing an opinion on the effectiveness of the Company's internal control over financial reporting. Accordingly, we express no such opinion.

Our audits included performing procedures to assess the risks of material misstatement of the consolidated financial statements, whether due to error or fraud, and performing procedures that respond to those risks. Such procedures included examining, on a test basis, evidence regarding the amounts and disclosures in the consolidated financial statements. Our audits also included evaluating the accounting principles used and significant estimates made by management, as well as evaluating the overall presentation of the consolidated financial statements. We believe that our audits provide a reasonable basis for our opinion.

**Critical Audit Matters**

The critical audit matters communicated below are matters arising from the current period audit of the consolidated financial statements that were communicated or required to be communicated to the audit committee and that: (1) relate to accounts or disclosures that are material to the consolidated financial statements and (2) involved our especially challenging, subjective, or complex judgments. The communication of the critical audit matters does not alter in any way our opinion on the consolidated financial statements, taken as a whole, and we are not, by communicating these critical audit matters below, providing separate opinions on the critical audit matters or on the accounts or disclosures to which they relate.

[**Table of Contents**](#TOC)

**Revenue Recognition – Determination of Transaction Price for Product Revenue**

As described in Note 1 to the consolidated financial statements, the Company recognizes product revenue upon completion of the test and delivery of results to the physician based on estimates of the amounts that will ultimately be realized. When determining the amount of revenue to be recognized for a delivered test result, management applies judgment to determine the transaction price, which affects the amount of revenue recognized. The Company's product revenue for the year ended December 31, 2025 was $9.2 million.

We identified the auditing of management's determination of the transaction price as a critical audit matter. Management's determination of the transaction price considers certain inputs, such as payment history, including amount and timing of payment and payer coverage. Auditing these inputs required challenging auditor judgment due to the nature and extent of audit effort required.

The primary procedures we performed to address this critical audit matter included:

● Assessing the reasonableness of management's inputs used to estimate the transaction price by testing on a sample basis the underlying data by:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;o Inspecting the supporting documentation related to the determination of payer grouping, and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;o Reviewing historical cash collections used in determining the transaction price.

● Recalculating the average unit price using the historical cash collections for the period for each payer group.

**Accounting for March 2025 Convertible Note Agreement**

As described in Note 7 to the consolidated financial statements, the Company entered into a convertible note agreement (the Notes) with certain existing shareholders for the issuance and sale of the Notes for an aggregate principal amount of $1.4 million. On March 12, 2025, the Notes were converted into shares of common stock and warrants pursuant to the terms of agreement.

We identified the assessment of the accounting related to the conversion of the Notes as a critical audit matter due to the complexity of the transaction, which requires management to make significant judgments in the interpretation of the terms of the agreements in applying the appropriate accounting guidance. Auditing management's application of the appropriate accounting guidance required challenging and complex auditor judgment due to the nature and extent of audit effort required, including the use of firm personnel with expertise in technical accounting.

The primary procedures we performed to address this critical audit matter included:

● Reviewing and analyzing the terms of the agreements included in management's assessment of the accounting of the Notes.

● Utilizing firm personnel with expertise in the relevant technical accounting to assist in evaluating (i) the relevant contract terms of the issuances in relation to the appropriate accounting guidance, and (ii) the appropriateness of conclusions reached by the Company.

/s/ BDO USA, P.C.

We have served as the Company's auditor since 2012.

Boston, Massachusetts

April 1, 2026

[**Table of Contents**](#TOC)

**Aspira Women's Health Inc.**

**Consolidated Balance Sheets**

(Amounts in Thousands, Except Share and Par Value and Per Share Amounts)

---

| | | |
|:---|:---|:---|
|  | **December 31,** <br>**2025** | **December 31,** <br>**2024** |
| **Assets** |  |  |
| Current assets: |  |  |
| &nbsp;&nbsp;Cash and cash equivalents | $1755 | $1769 |
| &nbsp;&nbsp;Accounts receivable, net of reserves of $44 | 1207 | 990 |
| &nbsp;&nbsp;Prepaid expenses and other current assets | 845 | 1098 |
| &nbsp;&nbsp;Inventories | 256 | 326 |
| &nbsp;&nbsp;Total current assets | 4063 | 4183 |
| Property and equipment, net | 47 | 69 |
| Intangible assets, net | 310 | - |
| Right-of-use assets | 980 | 1194 |
| Other assets | 56 | 45 |
| &nbsp;&nbsp;Total assets | $5456 | $5491 |
| **Liabilities and Stockholders' Deficit** |  |  |
| Current liabilities: |  |  |
| &nbsp;&nbsp;Accounts payable | $1237 | $2173 |
| &nbsp;&nbsp;Accrued liabilities | 1297 | 2445 |
| &nbsp;&nbsp;Current portion of long-term debt | 236 | 229 |
| &nbsp;&nbsp;Short-term debt | 493 | 614 |
| &nbsp;&nbsp;Current maturities of lease liabilities | 202 | 7 |
| &nbsp;&nbsp;Total current liabilities | 3465 | 5468 |
| Non-current liabilities: |  |  |
| &nbsp;&nbsp;Long-term debt | 1041 | 1278 |
| &nbsp;&nbsp;Non-current maturities of lease liabilities | 1008 | 1248 |
| &nbsp;&nbsp;Warrant liabilities | 6874 | 60 |
| &nbsp;&nbsp;Total liabilities | 12388 | 8054 |
| Commitments and contingencies (Note 7) |  |  |
| Stockholders' deficit: |  |  |
| &nbsp;&nbsp;Preferred stock, par value $0.001 per share, 5,000,000 shares authorized; no shares issued and outstanding at December 31, 2025 and December 31, 2024 | - | - |
| &nbsp;&nbsp;Common stock, par value $0.001 per share, 200,000,000 shares authorized at December 31, 2025 and December 31, 2024; 43,480,411 and 17,407,120 shares issued and outstanding at December 31, 2025 and December 31, 2024, respectively | 43 | 17 |
| &nbsp;&nbsp;Additional paid-in capital | 537202 | 528817 |
| &nbsp;&nbsp;Accumulated deficit | (544177) | (531397) |
| &nbsp;&nbsp;Total stockholders' deficit | (6932) | (2563) |
| &nbsp;&nbsp;Total liabilities and stockholders' deficit | $5456 | $5491 |

---

See accompanying notes to consolidated financial statements

[**Table of Contents**](#TOC)

**Aspira Women's Health Inc.**

**Consolidated Statements of Operations**

(Amounts in Thousands, Except Share and Per Share Amounts)

---

| | | |
|:---|:---|:---|
|  | **Year Ended**  | **Year Ended**  |
|  | **December 31,**  | **December 31,**  |
|  | **2025** | **2024** |
| Revenue: |  |  |
| &nbsp;&nbsp;Product | $9216 | $9182 |
| Total revenue | 9216 | 9182 |
| Cost of revenue: |  |  |
| &nbsp;&nbsp;Product | 3301 | 3703 |
| Total cost of revenue | 3301 | 3703 |
| Gross profit | 5915 | 5479 |
| Operating expenses: |  |  |
| &nbsp;&nbsp;Research and development | 2878 | 3266 |
| &nbsp;&nbsp;Sales and marketing | 2986 | 8146 |
| &nbsp;&nbsp;General and administrative | 7964 | 10345 |
| Total operating expenses | 13828 | 21757 |
| Loss from operations | (7913) | (16278) |
| Other (expense) income, net: |  |  |
| &nbsp;&nbsp;Change in fair value of warrant liabilities | (5607) | 1346 |
| &nbsp;&nbsp;Change in fair value of convertible notes | 170 | - |
| &nbsp;&nbsp;Loss upon issuance of convertible notes carried at fair value | (1198) | - |
| &nbsp;&nbsp;Interest expense, net | (41) | (33) |
| &nbsp;&nbsp;Other income (expense), net | 1809 | 1871 |
| Total other (expense) income, net | (4867) | 3184 |
| Net loss | $(12780) | $(13094) |
| Net loss per share - basic and diluted | $(0.37) | $(0.93) |
| Weighted average common shares used to compute basic and diluted net loss per common share | 34102760 | 14134626 |

---

See accompanying notes to consolidated financial statements

[**Table of Contents**](#TOC)

**Aspira Women's Health Inc.**

**Consolidated Statements of Changes in Stockholders' Deficit**

(Amounts in Thousands, Except Share Amounts)

---

| | | | | | |
|:---|:---|:---|:---|:---|:---|
|  | **Common Stock** | **Common Stock** | | | |
|  | **Shares** | **Amount** | **Additional**<br>**Paid-In**<br>**Capital** | <br>**Accumulated**<br>**Deficit** | **Total**<br>**Stockholders'**<br>**Deficit** |
| **Balance at December 31, 2023** | 10645049 | $11 | $515927 | $(518303) | $(2365) |
| &nbsp;&nbsp;Net loss | - | - | - | (13094) | (13094) |
| &nbsp;&nbsp;Common stock issued under 2023 Equity Line of Credit Agreement | 949574 | 1 | 1900 | - | 1901 |
| &nbsp;&nbsp;Common stock issued under 2024 Direct Offering, net of issuance costs of $733 | 1371000 | 1 | 4829 | - | 4830 |
| &nbsp;&nbsp;Common stock issued under Warrant Inducement Agreement, net of issuance costs of $277 | 1711111 | 2 | 1860 | - | 1862 |
| &nbsp;&nbsp;Reclassification of Warrant Liability upon Exercise | - | - | 245 | - | 245 |
| &nbsp;&nbsp;Common stock issued under 2024 At the Market Agreement, net of issuance costs of $189 | 1073050 | 1 | 714 | - | 715 |
| &nbsp;&nbsp;Common stock issued under 2024 Private Placement Offering, net of issuance costs of $72 | 1248527 | 1 | 1837 | - | 1838 |
| &nbsp;&nbsp;Common stock issued under 2024 Securities Purchase Agreements | 9733 | - | 11 | - | 11 |
| &nbsp;&nbsp;Warrant Exercise | 200000 | - | - | - | - |
| &nbsp;&nbsp;Common stock issued for vested restricted stock awards | 199076 | - | 161 | - | 161 |
| &nbsp;&nbsp;Stock-based compensation expense | - | - | 1333 | - | 1333 |
| **Balance at December 31, 2024** | 17407120 | 17 | 528817 | (531397) | (2563) |
| &nbsp;&nbsp;Net loss | - | - | - | (12780) | (12780) |
| &nbsp;&nbsp;Common stock issued under 2024 At-the-Market Offering Agreement, net of issuance costs | 12277441 | 12 | 3325 | - | 3337 |
| &nbsp;&nbsp;Common stock issued for March 2025 Convertible Notes settlement | 5465850 | 6 | 910 | - | 916 |
| &nbsp;&nbsp;Common stock issued under 2025 Equity Purchase Agreement, net of issuance costs | 354988 | 1 | 24 | - | 25 |
| &nbsp;&nbsp;Common stock issued under September 2025 Private Placement Offering, net of issuance costs | 6550000 | 7 | 2802 | - | 2809 |
| &nbsp;&nbsp;Common stock issued for exercised warrants | 450000 | - | 381 | - | 381 |
| &nbsp;&nbsp;Common stock issued for entering into 2025 Lincoln Park Equity Line of Credit | 824493 | - | 412 | - | 412 |
| &nbsp;&nbsp;Common stock issued for vested restricted stock awards | 150519 | - | 102 | - | 102 |
| &nbsp;&nbsp;Stock-based compensation expense | - | - | 429 | - | 429 |
| **Balance at December 31, 2025** | 43480411 | $43 | $537202 | $(544177) | $(6932) |

---

See accompanying notes to consolidated financial statements

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**Aspira Women's Health Inc.**

**Consolidated Statements of Cash Flows**

(Amounts in Thousands)

---

| | | |
|:---|:---|:---|
|  | **Year Ended**  | **Year Ended**  |
|  | **December 31,**  | **December 31,**  |
|  | **2025** | **2024** |
| **Cash flows from operating activities:** |  |  |
| Net loss | $(12780) | $(13094) |
| Adjustments to reconcile net loss to net cash used in operating activities: |  |  |
| &nbsp;&nbsp;Non-cash lease expense | 169 | 3 |
| &nbsp;&nbsp;Depreciation and amortization | 112 | 92 |
| &nbsp;&nbsp;Stock-based compensation expense | 531 | 1494 |
| &nbsp;&nbsp;Change in fair value of warrant liabilities | 5607 | (1346) |
| &nbsp;&nbsp;Change in fair value of March 2025 Convertible Notes | (170) | - |
| &nbsp;&nbsp;Loss upon issuance of March 2025 Convertible Notes carried at fair value | 1198 | - |
| &nbsp;&nbsp;Loss on impairment and disposal of property and equipment | 1 | 41 |
| &nbsp;&nbsp;Financing expense for entering into 2025 Lincoln Park Equity Line of Credit | 412 | - |
| Changes in operating assets and liabilities: |  |  |
| &nbsp;&nbsp;Accounts receivable | (217) | 469 |
| &nbsp;&nbsp;Prepaid expenses and other assets | 242 | (115) |
| &nbsp;&nbsp;Inventories | 68 | (99) |
| &nbsp;&nbsp;Accounts payable | (936) | 912 |
| &nbsp;&nbsp;Accrued liabilities | (1148) | (418) |
| &nbsp;&nbsp;Other liabilities | (118) | (52) |
| Net cash used in operating activities | (7029) | (12113) |
| **Cash flows from investing activities:** |  |  |
| &nbsp;&nbsp;Purchase of property and equipment | (16) | (37) |
| &nbsp;&nbsp;Purchase of intangible assets | (385) | - |
| Net cash used in investing activities | (401) | (37) |
| **Cash flows from financing activities:** |  |  |
| &nbsp;&nbsp;Principal repayment of DECD loan | (233) | (93) |
| &nbsp;&nbsp;Proceeds from 2023 Equity Line of Credit Agreement | - | 1901 |
| &nbsp;&nbsp;Proceeds from 2024 Direct Offering | - | 5563 |
| &nbsp;&nbsp;Payment of issuance costs for 2024 Direct Offering | - | (733) |
| &nbsp;&nbsp;Proceeds from Warrant Inducement Agreement | - | 2139 |
| &nbsp;&nbsp;Payment of issuance costs for Warrant Inducement Agreement | - | (277) |
| &nbsp;&nbsp;Proceeds from 2024 Securities Purchase Agreements | - | 11 |
| &nbsp;&nbsp;Proceeds from 2024 At the Market Offering Agreement | 3484 | 904 |
| &nbsp;&nbsp;Payment of issuance costs for 2024 At the Market Offering Agreement | (147) | (189) |
| &nbsp;&nbsp;Proceeds from 2024 Private Placement Offering | - | 1910 |
| &nbsp;&nbsp;Payment of issuance costs for 2024 Private Placement Offering | - | (72) |
| &nbsp;&nbsp;Proceeds from 2025 Private Placement Offering | 2949 | - |
| &nbsp;&nbsp;Payment of issuance costs for 2025 Private Placement Offering | (140) | - |
| &nbsp;&nbsp;Proceeds from issuance of common stock from exercise of warrants | 112 | - |
| &nbsp;&nbsp;Proceeds from March 2025 Convertible Notes | 1366 | - |
| &nbsp;&nbsp;Proceeds from 2025 Equity Purchase Agreement | 25 | - |
| Net cash provided by financing activities | 7416 | 11064 |
| Net decrease in cash and cash equivalents  | (14) | (1086) |
| Cash and cash equivalents , beginning of year | 1769 | 2855 |
| Cash and cash equivalents , end of year | $1755 | $1769 |
| **Supplemental disclosure of cash flow information:** |  |  |
| &nbsp;&nbsp;Cash paid for interest | $34 | $49 |
| **Supplemental disclosure of noncash investing and financing activities:** |  |  |
| &nbsp;&nbsp;Increase in right-of-use assets | $- | $895 |
| &nbsp;&nbsp;Fair value of warrants issued in conjunction with Warrant Inducement Agreement | $- | $1323 |
| &nbsp;&nbsp;Fair value of warrants issued upon conversion of March 2025 Convertible Notes | $1477 | $- |
| &nbsp;&nbsp;Fair value of common stock issued upon conversion of March 2025 Convertible Notes | $916 | $- |

---

See accompanying notes to consolidated financial statements

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**Aspira Women's Health Inc.**

Notes to Consolidated Financial Statements

#### NOTE 1: BASIS OF PRESENTATION AND SUMMARY OF SIGNIFICANT ACCOUNTING AND REPORTING POLICIES

#### Organization and Basis of Presentation
Aspira Women's Health Inc. ("Aspira," and together with its wholly-owned subsidiaries, the "Company") is incorporated in the state of Delaware, and is engaged in the business of developing and commercializing diagnostic tests for gynecologic disease. The Company currently markets and sells the following products and related services: (1) the Ova1Plus workflow, which uses Ova1, a qualitative serum test intended as an aid to further assess the likelihood of malignancy in women with an ovarian adnexal mass for which surgery is planned when the physician's independent clinical and radiological evaluation does not indicate malignancy, as the primary test and Overa, a second-generation biomarker test intended to maintain Ova1's high sensitivity while improving specificity, as a reflex for Ova1 intermediate range results, leveraging the strengths of Ova1's MIA sensitivity and Overa's (MIA2G) specificity to reduce incorrectly elevated results; and (2) OvaWatch, an LDT intended to assist in the initial clinical assessment of malignancy risk in all women thought to have an indeterminate or benign adnexal mass. Overa is currently not offered except as a reflex test performed as part of the Ova1Plus workflow. Collectively, these tests are referred to and marketed as OvaSuite.

The Company's products are distributed through its direct national sales force, including field sales and inside sales. OvaSuite tests are marketed to both physicians via a direct referral to Aspira as well as to laboratories via the Aspira Synergy platform. The Company also engages in channel distribution partnerships by which OvaSuite is distributed via laboratory partners such as BioReference Health, LLC. and ARUP.

#### Going Concern
The Company has incurred significant net losses and negative cash flows from operations since inception, and as a result has an accumulated deficit of approximately $544,177,000 and working capital of $598,000 as of December 31, 2025. For the year ended December 31, 2025, the Company incurred a net loss of $12,780,000 and used cash in operations of $7,029,000. The Company also expects to incur a net loss and negative cash flows from operations for 2026. In order to continue its operations as currently planned through 2026 and beyond, the Company will need to raise additional capital. The Company expects to take further action to protect its liquidity position. Such actions may include, but are not limited to:

● Raising capital through an equity offering either in the public markets or via a private placement offering (to the extent that the Company raises additional funds by issuing equity securities, the Company's stockholders may experience significant dilution. However, no assurance can be given that capital will be available on acceptable terms, or at all);

● Securing debt, however, no assurance can be given that debt will be available on acceptable terms or at all;

● Reducing executive bonuses or replacing cash compensation with equity grants;

● Reducing professional services and consulting fees and eliminating non-critical projects;

● Reducing travel and entertainment expenses; and

● Reducing, eliminating or deferring discretionary marketing programs.

The Company also has outstanding warrants to purchase shares of its common stock that may be exercised; although there can be no assurance that the warrants will be exercised.

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There can be no assurance that the Company will achieve or sustain profitability or positive cash flow from operations. Management expects cash from product sales and licensing to be the Company's only material, recurring source of cash in 2026. Given the above conditions, there is substantial doubt about the Company's ability to continue as a going concern within one year after the date these financial statements are filed. The consolidated financial statements have been prepared on a going concern basis and do not include any adjustments that might result from these uncertainties.

On April 15, 2025, the Company received written notice from the Office of General Counsel of The Nasdaq Stock Market ("Nasdaq") indicating that the Nasdaq Hearings Panel determined to delist the Company's shares from Nasdaq due to the Company's failure to meet Nasdaq's continued listing standard under Nasdaq Listing Rule 5550(b)(1), which required the Company to maintain a minimum of $2.5 million in stockholders' equity for continued listing on the Nasdaq Capital Market. The Company's common stock was traded on the OTC Markets Group effective at the open of trading on April 17, 2025 under the symbol "AWHL." The Company's common stock is currently traded on the OTC QX Best Market.

#### Basis of Consolidation
The consolidated financial statements include the accounts of the Company and its wholly-owned subsidiaries. All intercompany transactions have been eliminated in consolidation.

#### Segment Reporting
The Company's Chief Operating Decision Maker ("CODM") is its Chief Executive Officer, who reviews financial information presented on a consolidated basis for purposes of allocating resources and assessing financial performance. The Company views its operations and manages its business in a single operating segment, which is the discovery, development, and commercialization of noninvasive diagnostic tests. As a result, the CODM evaluates the business on a consolidated basis. The Company's CODM uses the net loss that is reported on the Company's consolidated statement of operations as a consolidated net loss for the purpose of allocating resources. The Company also monitors its cash and cash equivalents as reported on its consolidated balance sheets to determine its liquidity needs and to allocate resources.

The accounting policies of the Company's single segment are the same as those described in this summary of significant accounting policies. The CODM assesses the performance of the Company's single segment and decides how to allocate resources based on consolidated net income. Under the current organizational structure, this measure is not discreetly available or required individually for any of the Company's business activities and is only available at the consolidated level. The monitoring of budgeted versus actual results are used in assessing performance of the Company's single segment, allocating resources and in establishing management's compensation. The measure of segment assets is reported on the consolidated balance sheet as total consolidated assets. Consolidated revenue does not include any inter-segment sales or transfers.

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The following table summarizes financial statement line items regularly provided to the CODM (in thousands).

---

| | | |
|:---|:---|:---|
| | **December 31,**  | **December 31,**  |
| <br>**(in thousands)** | **2025** | **2024** |
| Total Assets | $5456 | $5491 |
| Total Revenue | $9216 | $9182 |
| Less: |  |  |
| &nbsp;&nbsp;&nbsp;&nbsp;Personnel costs | (6994) | (13042) |
| &nbsp;&nbsp;&nbsp;&nbsp;Professional fees | (4127) | (5191) |
| &nbsp;&nbsp;&nbsp;&nbsp;Postage and kit costs | (1341) | (1358) |
| &nbsp;&nbsp;&nbsp;&nbsp;Travel expenses | (235) | (1041) |
| &nbsp;&nbsp;&nbsp;&nbsp;Lab related expenses | (1620) | (1455) |
| &nbsp;&nbsp;&nbsp;&nbsp;Software licenses and maintenance costs | (691) | (795) |
| &nbsp;&nbsp;&nbsp;&nbsp;Other segment item<sup>(1)</sup> | (2121) | (2579) |
| &nbsp;&nbsp;&nbsp;&nbsp;Other (expense) income, net | (4867) | 3185 |
| Net Loss | $(12780) | $(13094) |

---

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(1) Other segment items include overhead costs, insurance expenses, IT expenses and depreciation.

#### Use of Estimates
The preparation of consolidated financial statements in accordance with generally accepted accounting principles in the U.S. ("GAAP") requires management to make estimates and assumptions that affect the amounts reported in the consolidated financial statements and accompanying notes. The primary estimates underlying the Company's consolidated financial statements include assumptions regarding revenue recognition as well as variables used in calculating the fair value of the Company's equity awards, warrants, income taxes and contingent liabilities. Changes in estimates and assumptions are reflected in reported results in the period in which they become known. Actual results could differ from those estimates.

#### Cash and Cash Equivalents
Cash and cash equivalents consist of cash and highly liquid investments with maturities of three months or less from the date of purchase, which are readily convertible into known amounts of cash and are so near to their maturity that they present an insignificant risk of changes in value because of interest rate changes. Highly liquid investments that are considered cash equivalents include money market funds.

#### Fair Value Measurements
Accounting Standards Codification ("ASC") Topic 820, *Fair Value and Measurements* ("ASC 820"), defines fair value as the exchange price that would be received for an asset or paid to transfer a liability (an exit price) in the principal or most advantageous market for the asset or liability in an orderly transaction between market participants on the measurement date. ASC 820 also establishes a fair value hierarchy which requires an entity to maximize the use of observable inputs and minimize the use of unobservable inputs when measuring fair value. The standard describes three levels of inputs that may be used to measure fair value:

Level 1 – Quoted prices in active markets for identical assets or liabilities.

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Level 2 – Observable inputs other than Level 1 prices such as quoted prices for similar assets or liabilities, quoted prices in markets that are not active, or other inputs that are observable or can be corroborated by observable market data for substantially the full term of the assets or liabilities.

Level 3 – Unobservable inputs that are supported by little or no market activity and that are significant to the fair value of the assets or liabilities.

If a financial instrument uses inputs that fall within different levels of the hierarchy, the instrument will be categorized based upon the lowest level of input that is significant to the fair value calculation.

Financial instruments of the Company consist primarily of cash and cash equivalents, accounts receivable, accounts payable, debt and warrant liabilities.

The estimated fair value of financial instruments has been determined using available market information or other appropriate valuation methodologies. However, considerable judgment is required in interpreting market data to develop estimates of fair value; therefore, the estimates are not necessarily indicative of the amounts that could be realized or would be paid in a current market exchange. The effect of using different market assumptions and/or estimation methodologies may be material to the estimated fair value amounts.

Cash and cash equivalents, accounts receivable, and accounts payable are considered Level 1 and are carried at cost due to their short-term nature and their market interest rates. Warrant liabilities are considered Level 2 or Level 3 and are recorded at fair value at the end of each reporting period. Debt is considered Level 3, which the Company does not record at fair value. The carrying value of debt approximates fair value due to its interest rate approximating market rates of interest available to the Company for similar instruments.

#### Concentration of Credit Risk
Financial instruments that potentially subject the Company to a concentration of credit risk consist of cash and cash equivalents and accounts receivable. The Company maintains cash and cash equivalents in recognized financial institutions in the United States. The funds are insured by the FDIC up to a maximum of $250,000 but are otherwise unprotected. The Company has not experienced any losses associated with deposits of cash and cash equivalents. The Company does not invest in derivative instruments or engage in hedging activities.

#### Accounts Receivable
Virtually all accounts receivable are derived from sales made to customers located in North America. The Company grants credit to customers in the normal course of business and the resulting trade receivables are stated at their net realizable value. The Company maintains an allowance for credit losses based upon the expected collectability of accounts receivable, such as the historical collection cycle. Amounts are written off against the allowances for credit losses when the Company determines that a customer account is not collectable. We believe our exposure to concentrations of credit risk is limited due to the diversity of our payer base.

#### Inventory
The Company has inventory consisting primarily of kit inventory for specimen delivery as well as reagents used for specimen testing and miscellaneous inventory such as pipettes, gloves and other non-reagent items.

At each reporting period the Company reviews its inventories for obsolescence and writes down obsolete or otherwise unmarketable inventory to its estimated net realized value, which is primarily related to kit inventory when kits expire. Inventory is valued at cost using the first-in-first-out method.

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#### Property and Equipment
Property and equipment are carried at cost less accumulated depreciation and amortization. Property and equipment are depreciated when placed into service using the straight-line method over the estimated useful lives, generally three to five years. Leasehold improvements are amortized using the straight-line method over the shorter of the estimated useful life of the asset or the remaining term of the lease. Maintenance and repairs are charged to operations as incurred. Upon sale or retirement of assets, the cost and related accumulated depreciation are removed from the balance sheet and the resulting gain or loss is reflected in operations.

Property and equipment are reviewed for impairment when events or changes in circumstances indicate the carrying amount of an asset may not be recoverable. If property and equipment are considered to be impaired, an impairment loss is recognized.

#### Revenue Recognition
*Product Revenue – OvaSuite:* The Company recognizes product revenue in accordance with the provisions of ASC Topic 606, *Revenue from Contracts with Customers* ("ASC 606"). Product revenue is recognized upon completion of the OvaSuite test and delivery of results to the physician based on estimates of amounts that will ultimately be realized. In determining the amount of revenue to be recognized for a delivered test result, the Company considers factors such as payment history and amount, payer coverage, whether there is a reimbursement contract between the payer and the Company, and any developments or changes that could impact reimbursement. These estimates require significant judgment by management as the collection cycle on some accounts can be as long as one year. The effect of any change made to an estimated input component and, therefore revenue recognized, would be recorded as a change in estimate at the time of the change.

The Company also reviews its patient account population and determines an appropriate distribution of patient accounts by payer (i.e., Medicare, patient pay, other third-party payer, etc.) into portfolios with similar collection experience. The Company has elected this practical expedient that, when evaluated for collectability, results in a materially consistent revenue amount for such portfolios as if each patient account were evaluated on an individual contract basis. For the years ended December 31, 2025 and 2024, there were no adjustments to estimates of variable consideration to derecognize revenue for services provided in a prior period; however, additional revenue of approximately $343,000 and $4,000 was recognized for the years ended December 31, 2025 and 2024, respectively. There were no impairment losses on accounts receivable recorded for the years ended December 31, 2025 and 2024.

#### Research and Development Costs
Research and development costs are expensed as incurred. Research and development costs consist primarily of payroll and related costs, materials and supplies used in the development of new products, and fees paid to third parties that conduct certain research and development activities on behalf of the Company. In addition, acquisitions of assets to be consumed in research and development, with no alternative future use, are expensed as incurred as research and development costs. Software development costs incurred in the research and development of new products are expensed as incurred until technological feasibility is established.

#### Patent Costs
Costs incurred in filing, prosecuting and maintaining patents (principally legal fees) are expensed as incurred and recorded within general and administrative expenses on the Company's consolidated statements of operations. Such costs aggregated to approximately $261,000 and $274,000 for the years ended December 31, 2025 and 2024, respectively.

#### Stock-Based Compensation
The Company records the fair value of non-cash stock-based compensation costs for stock options related to the 2019 Stock Incentive Plan ("2019 Plan"). The Company estimates the fair value of stock options using a Black-Scholes option valuation model. This model requires the input of subjective assumptions including expected stock price volatility,

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expected life and estimated forfeitures of each award. These assumptions consist of estimates of future market conditions, which are inherently uncertain, and therefore are subject to management's judgment. The Company uses the straight-line method to amortize the fair value over the requisite service period of the award, which is generally equal to the vesting period.

The expected life of options is based on historical data of actual experience with the options granted and represents the period of time that the options granted are expected to be outstanding. This data includes employees' expected exercise and post-vesting employment termination behaviors. The expected stock price volatility is estimated using Company historical volatility in deriving the expected volatility assumption. The Company made an assessment that Company historic volatility is most representative of future stock price trends. The expected dividend yield is based on the estimated annual dividends that are expected to be paid over the expected life of the options as a percentage of the market value of the Company's common stock as of the grant date. The risk-free interest rate for the expected life of the options granted is based on the United States Treasury yield curve in effect as of the grant date. The Company records stock-based compensation net of estimated forfeitures.

#### Government Assistance
On March 27, 2020, the U.S. government enacted the Coronavirus Aid, Relief, and Economic Security Act ("CARES Act"). One provision within the CARES Act provided an *Employee Retention Credit* ("ERC"), which allows for employers to claim a refundable tax credit against the employer share of Social Security tax equal to 50% of the qualified wages paid to employees from March 13, 2020 through December 31, 2020. The ERC was subsequently expanded in 2021 for employers to claim a refundable tax credit for 70% of the qualified wages paid to employees from January 1, 2021 through September 30, 2021.

The Company qualified for federal government assistance through the ERC. For the years ended December 31, 2025 and 2024, the Company received approximately $1,013,000 and $38,000, respectively, from the Internal Revenue Service for payroll tax refunds for 2020 and 2021. The Company recorded the receipt as other income in its accompanying consolidated statements of operations.

On October 23, 2024, the Advanced Research Projects Agency for Health ("ARPA-H") announced that it had selected the Company as an awardee of the Sprint for Women's Health. As an awardee, the Company could have received $10,000,000 in funding over two years through the Sprint for Women's Health launchpad track for later-stage health solutions. The Company was entitled to payments based on the completion of certain agreed-upon milestones. The award also provided for access to advisors to support the successful completion and commercial launch of the test before the end of the two-year contract term.

The Company met the first milestone for payment in the fourth quarter of 2024 and received a payment of $2,000,000. The second milestone was met during the first quarter of 2025 and the Company received a payment of $1,500,000, which is recognized in Other expense (income), net in the consolidated statement of operations for the year ended December 31, 2025.

On June 9, 2025, Aspira received notice from ARPA-H that ARPA-H and the assigned managing contractor, VentureWell, have determined that Aspira had not met the specifications of the third milestone, and have therefore elected to terminate the ENDOinform development program contract. The Company continues to work on ENDOinform and is endeavoring to have the award reinstated.

#### Contingencies
The Company accounts for contingencies in accordance with ASC 450, *Contingencies* ("ASC 450"), which requires that an estimated loss from a loss contingency be accrued when (i) information available prior to issuance of the financial statements indicates that it is probable that an asset has been impaired or a liability has been incurred at the date of the financial statements and (ii) when the amount of the loss can be reasonably estimated. Accounting for contingencies such as legal and contract dispute matters requires the use of management's judgment. Management believes that the

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Company's accruals for these matters are adequate. Nevertheless, the actual loss from a loss contingency might differ from management's estimates.

#### Income Taxes
The Company accounts for income taxes using the asset and liability method. Under this method, deferred tax assets and liabilities are determined based on the difference between the financial statement and the tax bases of assets and liabilities using the current tax laws and rates. A valuation allowance is established when necessary to reduce deferred tax assets to the amounts more likely than not expected to be realized.

ASC Topic 740, *Accounting for Uncertainty in Income Taxes,* clarifies the accounting for uncertainty in income taxes recognized in the financial statements and provides that a tax benefit from an uncertain tax position may be recognized when it is more likely than not that the position will be sustained upon examination, including resolutions of any related appeals or litigation processes, based on the technical merits. This interpretation also provides guidance on measurement, derecognition, classification, interest and penalties, accounting in interim periods, and disclosure.

The Company recognizes interest and penalties related to unrecognized tax benefits within the interest expense line and other expense line, respectively, in its consolidated statements of operations. Accrued interest and penalties are included within the related liability lines in the Company's consolidated balance sheet.

#### Net Loss Per Share
Basic net loss per share is computed by dividing the net loss by the weighted average number of shares of common stock outstanding during the period. Diluted loss per share is computed by dividing the net loss by the weighted average number of shares of common stock adjusted for the dilutive effect of common stock equivalent shares outstanding during the period. Common stock equivalents consist of stock options, restricted stock units and stock warrants. Common equivalent shares are excluded from the computation in periods in which they have an anti-dilutive effect on earnings per share.

#### Leases
The Company determines if a contract, at its inception, is a lease or contains a lease based on whether the contract conveys the right to control the use of identified property, plant, or equipment (an identified asset) for a period of time in exchange for consideration. To determine whether a contract conveys the right to control the use of an identified asset for a period of time, the Company assesses whether, throughout the period of use, it has both the right to obtain substantially all of the economic benefits from use of the identified asset, and the right to direct the use of the identified asset.

Lease classification, recognition, and measurement are then determined at the lease commencement date. For arrangements that contain a lease the Company (i) identifies lease and non-lease components, (ii) determines the consideration in the contract, (iii) determines whether the lease is an operating or financing lease; and (iv) recognizes lease right-of-use assets and liabilities. Lease liabilities and their corresponding right-of-use assets are recorded based on the present value of lease payments over the expected lease term. The interest rate implicit in lease contracts is typically not readily determinable and as such, the Company uses an incremental borrowing rate based on the information available at the lease commencement date, which represents a rate that would be incurred to borrow, on a collateralized basis, over a similar term, an amount equal to the lease payments in a similar economic environment.

The Company enters into contracts that contain both lease and non-lease components. Non-lease components may include items such as maintenance, utilities, or other operating costs. For leases of real estate, the Company combines the lease and associated non-lease components in its lease arrangements as a single lease component. Variable costs, such as utilities or maintenance costs, are not included in the measurement of right-of-use assets and lease liabilities, but rather are expensed when the event determining the amount of variable consideration to be paid occurs.

Additionally, the Company has elected the short-term lease exemption and, therefore, does not recognize a right-of-use asset or corresponding liability for lease arrangements with an original term of 12 months or less.

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Operating leases are included in right-of-use operating assets, current lease liabilities, and noncurrent lease liabilities in the consolidated balance sheets as of December 31, 2025 and 2024.

#### NOTE 2: RECENT ACCOUNTING PRONOUNCEMENTS
***Recently Adopted Accounting Standards***

In June 2022, the FASB issued ASU No. 2022-03, *Fair Value Measurement (Topic 820): Fair Value Measurement of Equity Securities Subject to Contractual Sale Restrictions* ("ASU 2022-03") to clarify guidance in Topic 820 on the fair value measurement of an equity security that is subject to a contractual sale restriction and also requires specific disclosures related to an equity security. ASU 2022-03 was effective for fiscal years beginning after December 15, 2024, including interim periods within the fiscal year. Early adoption was permitted. The Company adopted ASU 2022-03 on January 1, 2025. The adoption of this standard did not result in a material impact on the Company's results of operations, financial position, or cash flows.

In March 2023, the FASB issued ASU No. 2023-01, *Leases (Topic 842): Common Control Arrangements* ("ASU 2023-01")*.* ASU 2023-01 clarified the accounting for leasehold improvements for leases under common control. The guidance was effective for fiscal years beginning after December 15, 2024, including interim periods within those fiscal years. Early adoption was permitted. ASU 2023-01 was adopted by The Company on January 1, 2025. The adoption of this standard did not have a material impact on the Company's results of operations, financial position, or cash flows.

In October 2023, the FASB issued ASU No. 2023-06, D*isclosure Improvements: Codification Amendments in Response to the SEC's Disclosure Update and Simplification Initiative* ("ASU 2023-06"). The amendments in this ASU are expected to clarify or improve disclosure and presentation requirements of a variety of ASC topics by aligning them with the SEC's regulations. ASU 2023-06 was effective for each amendment on the effective date of the SEC's corresponding disclosure rule changes. The adoption of ASU 2023-06 did not have a material impact on the Company's results of operations, financial position, or cash flows.

In December 2023, the FASB issued ASU No. 2023-09, *Income Taxes (Topic 740): Improvements to Income Tax Disclosures* ("ASU 2023-09") that addresses requests for improved income tax disclosures from investors that use the financial statements to make capital allocation decisions. Public entities were required to adopt the new guidance for fiscal years beginning after December 15, 2024. The amendments in this ASU must be applied on a retrospective basis to all prior periods presented in the financial statements and early adoption was permitted. The Company adopted ASU 2023-09 on January 1, 2025. The adoption of ASU 2023-09 did not have a material impact on its results of operations, financial position, or cash flows. For additional information, see Note 11, *Income Taxes*.

***Recently Issued Accounting Standards***

In November 2024, the FASB issued ASU No. 2024-03, *Income Statement – Reporting Comprehensive Income-Expense Disaggregation Disclosures (Subtopic 220-40): Disaggregation of Income Statement Expenses* ("ASU 2024-03") that requires disaggregation of certain expense captions into specified categories in disclosures within the footnotes to the financial statements. The standard will become effective for annual periods beginning after December 15, 2026, with early adoption permitted. The Company is currently evaluating the impact the adoption of ASU 2024-03 may have on its results of operations, financial position or cash flows.

In July 2025, the FASB issued ASU No. 2025-05, *Financial Instruments—Credit Losses (Topic 326): Measurement of Credit Losses for Accounts Receivable and Contract Assets* ("ASU 2025-05"). The guidance provides a practical expedient that all entities may elect to apply to accounts receivable and contract assets arising from transactions accounted for under ASC 606, *Revenue from Contracts with Customers*. ASU 2025-05 will allow entities to assume that current conditions as of the balance sheet date do not change for the remaining life of the assets when estimating expected credit losses for such assets. The update will be effective for the Company beginning after December 15, 2025, with early adoption permitted. The Company is currently evaluating the impact the adoption of ASU 2025-05 may have on its results of operations, financial position or cash flows.

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In September 2025, the FASB issued ASU No. 2025-06, *Targeted Improvements to the Accounting for Internal-Use Software* ("ASU 2025-06"), which clarifies and modernizes the accounting for costs related to internal-use software in *ASC Topic 350-40, Intangibles —Goodwill and Other — Internal-Use Software (ASC 350-40)*. ASU 2025-06 removed all references to project stages throughout ASC 350-40, and requires entities to begin capitalizing software costs when both of the following occur: (1) management, with the relevant authority, implicitly or explicitly authorizes and commits to funding a computer software project; and (2) it is probable that the project will be completed and the software will be used to perform the function intended (referred to as the probable-to-complete recognition threshold). The guidance is effective for annual periods beginning after December 15, 2027 and interim periods within those annual reporting periods. Early adoption is permitted. ASU 2025-06 can be either applied on a prospective basis, retrospective or modified prospective basis based on the status of the project and whether software costs were capitalized before the date of adoption. The Company is currently evaluating the impact the adoption of ASU 2025-06 may have on its results of operations, financial position or cash flows.

In December 2025, the FASB issued ASU 2025-11, *Interim Reporting (Topic 270): Narrow-Scope Improvements* ("ASU 2025-11"), which clarifies interim disclosure requirements by improving the navigability of the required interim disclosures and clarifying when that guidance is applicable. The standard is effective for interim reporting periods within annual reporting periods beginning after December 15, 2027. Early adoption is permitted. ASU 2025-11 can be applied either prospectively or retrospectively to any or all periods presented in the financial statements. The Company is currently evaluating the impact the adoption of ASU 2025-11 may have on its results of operations, financial position or cash flows.

In December 2025, the FASB issued ASU 2025-12, *Codification Improvements* ("ASU 2025-12"), which amends various areas of the Codification to enhance consistency and make the standards easier for preparers and users to interpret. ASU 2025-12 intends to refine and simplify the original guidance where it may have been unclear. ASU 2025-12 is effective for annual periods beginning after December 15, 2026, and interim periods within those annual periods, with early adoption permitted. Entities may adopt the guidance using a prospective or retrospective approach. The Company is currently evaluating the impact the adoption of ASU 2025-12 may have on its results of operations, financial position or cash flows.

#### NOTE 3: STRATEGIC ALLIANCE WITH QUEST DIAGNOSTICS INCORPORATED
In March 2015, the Company reached an agreement with Quest Diagnostics Incorporated ("Quest Diagnostics"). Pursuant to this agreement, all Ova1 U.S. testing services for Quest Diagnostics customers were transferred to Aspira's wholly-owned subsidiary, Aspira Labs, as of August 2015. Pursuant to this agreement, as amended as of July 18, 2025, Quest Diagnostics has continued to provide blood draw and logistics support by transporting specimens to Aspira Labs for testing and is billed by Quest Diagnostics for services performed. The purpose of the 2025 amendment was to add OvaWatch to the U.S testing services for Quest Diagnostics customers and to extend the term of the agreement from December 31, 2023 to December 31, 2025. Under the terms of the agreement, as amended, the Company is required to pay a monthly fee of $2,000 for the services of a part-time Quest Diagnostics project manager. As the contract has expired, the parties are currently negotiating a renewal agreement. As of December 31, 2025, the Company has $46,000 accrued and payable to Quest Diagnostics for phlebotomy services rendered on behalf of the Company under the terms of the agreements.

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#### NOTE 4: PROPERTY AND EQUIPMENT
The components of property and equipment as of December 31, 2025 and 2024 were as follows**:**

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| | | | |
|:---|:---|:---|:---|
| | | **December 31,**  | **December 31,**  |
| <br>**(in thousands)** | **Estimated**<br>**Useful Life** | **2025** | **2024** |
| Machinery and equipment | 3 - 5 years | $345 | $363 |
| Computer equipment and software | 3 years | 794 | 882 |
| Furniture and fixtures | 5 years | 148 | 150 |
| Leasehold improvements | <sup>(1)</sup> | 68 | 68 |
| &nbsp;&nbsp;Gross property and equipment |  | 1355 | 1463 |
| Accumulated depreciation and amortization |  | (1308) | (1394) |
| &nbsp;&nbsp;Property and equipment, net |  | $47 | $69 |

---

&nbsp;&nbsp;&nbsp;&nbsp;(1) Lesser of remaining lease term or estimated useful life

Depreciation expense for property and equipment was $37,000 and $92,000 for the years ended December 31, 2025 and 2024, respectively.

**NOTE 5: INTANGIBLE ASSETS**

During the year ended December 31, 2025, the Company purchased the software to provide integrated care plans for adnexal mass risk management. The Company classified the software as an Intangible asset.

Intangible assets as of December 31, 2025 and December 31, 2024 were as follows.

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| | | | |
|:---|:---|:---|:---|
| | | **December 31,** | **December 31,** |
| <br>**(in thousands)** | **Estimated**<br>**Useful Life** | **2025** | **2024** |
| Capitalized software | 3 years | 385 | - |
| Accumulated amortization  |  | (75) | - |
| &nbsp;&nbsp;Capitalized software, net  |  | $310 | $- |

---

Amortization expense for intangible assets was $75,000 and $0 for the years ended December 31, 2025 and 2024, respectively.

As of December 31, 2025, the annual amounts of amortization of the Company's intangible assets are shown in the table below.

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| | | | | |
|:---|:---|:---|:---|:---|
| | **Amortization by Period** | **Amortization by Period** | **Amortization by Period** | **Amortization by Period** |
| <br>**(in thousands)** | **Total** | **2026** | **2027** | **2028** |
| Estimated future amortization | $310 | $128 | $128 | $54 |
| &nbsp;&nbsp;Total | $310 | $128 | $128 | $54 |

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#### NOTE 6: ACCRUED LIABILITIES
The components of accrued liabilities as of December 31, 2025 and 2024 were as follows:

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| | | |
|:---|:---|:---|
| <br>**(in thousands)** | **December 31,** <br>**2025** | **December 31,** <br>**2024** |
| Payroll and benefits related expenses | $194 | $1448 |
| Collaboration and research agreements expenses | 266 | 228 |
| Professional services | 679 | 253 |
| Other accrued liabilities | 158 | 516 |
| &nbsp;&nbsp;Total accrued liabilities | $1297 | $2445 |

---

#### NOTE 7: COMMITMENTS, CONTINGENCIES AND DEBT
***Convertible Notes***

On March 5, 2025, the Company entered into a securities purchase agreement with certain existing accredited shareholders (the "Purchasers") for the issuance and sale in a private placement of an aggregate gross principal amount of $1,365,500 in the form of Convertible Notes (the "March 2025 Convertible Notes"). The Company incurred third-party issuance costs of $50,000 in connection with the March 2025 Convertible Notes which were expensed within general and administrative expenses on its unaudited condensed consolidated statement of operations for the year ended December 31, 2025. The March 2025 Convertible Notes were convertible into Units at a conversion price of $0.25 per Unit (the "Conversion Price"), with each Unit consisting of one share of common stock and 2.25 warrants (the "March 2025 Warrants") to purchase shares of common stock.

The Company elected to measure the March 2025 Convertible Notes using the fair value option under ASC 825 because the warrants issuable upon conversion of the March 2025 Convertible Notes are not indexed to the Company's stock. They include a variable exercise price that violates the fixed-for-fixed concept under ASC 815. Further, as the March 2025 Convertible Notes were issued at par (gross cash proceeds equaled the principal of the March 2025 Convertible Notes), the March 2025 Convertible Notes were not issued at a substantial premium and are eligible for the fair value option under ASC 825.

As the Company required the proceeds from the March 2025 Convertible Notes to sustain its operations, the terms were not favorable to the Company and the March 2025 Convertible Notes were issued as a discount. This resulted in the fair value of the March 2025 Convertible Notes upon issuance exceeding the proceeds received; and as such, the Company recognized the excess of approximately $1,198,000 in loss upon issuance of March 2025 Convertible Notes consolidated statement of operations during the year ended December 31, 2025.

The Company recorded the March 2025 Convertible Notes on its books at fair value. The fair value of the March 2025 Convertible Notes was calculated by backing out the fair value of the March 2025 Warrants, which was estimated using a Monte Carlo simulation pricing model. The Monte Carlo simulation pricing model uses Level 3 inputs due to its incorporation of significant inputs that are not observable in the market. The fair value of the convertible notes at issuance was approximately $2,563,000. Accordingly, on the date of issuance, the Company recorded the loss of $1,198,000, which is the difference between the fair value and the proceeds received. The March 2025 Convertible Notes were marked to market through the date of conversion, March 12, 2025. The change in fair value from the date of issuance through the date of conversion was a gain of $170,000 which was recorded as change in fair value of March 2025 Convertible Notes in the accompanying condensed consolidated financial statements.

The Purchasers of the March 2025 Convertible Notes are entitled to three representatives on the Company's board of directors.

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In addition, the Company granted the Purchasers of the March 2025 Convertible Notes certain customary registration rights with respect to the shares of common stock and shares of common stock underlying the March 2025 Warrants.

Pursuant to the terms of the agreement, the Company exercised its option to convert the March 2025 Convertible Notes into units at the Conversion Price when the sum of the net proceeds from the sale of the March 2025 Convertible Notes and the net proceeds from the sale of any shares of common stock and warrants by the Company subsequent to the March 2025 Convertible Notes exceeded $4,000,000. On March 11, 2025, the Company sold 9,476,837 shares of common stock under an at the market offering agreement for net proceeds of $2,703,000, which settled on March 12, 2025 and which combined with the $1,316,000 net proceeds from the March 2025 Private Placement exceeded the $4,000,000 requirement. As such, the March 2025 Convertible Notes, including interest accrued, for a total of $1,366,458 were converted into Units on March 12, 2025. A gain on the conversion of the March 2025 Convertible Notes of $170,000 was recorded as a change in fair value of March 2025 Convertible Notes in the Company's unaudited condensed consolidated statement of operations for the year ended December 31, 2025. See Note 8, *Common Stock*, for further discussion on the March 2025 Warrants.

#### Loan Agreement
On March 22, 2016, the Company entered into a loan agreement (as amended, the "DECD Loan Agreement") with the DECD, pursuant to which the Company may borrow up to $4,000,000 from the DECD. The loan bears interest at a fixed rate of 2.0% per annum and requires equal monthly payments of principal and interest until maturity, which would have occurred on April 15, 2026. As security for the loan, the Company has granted the DECD a blanket security interest in the Company's personal and intellectual property. The DECD's security interest in the Company's intellectual property may be subordinated to a qualified institutional lender.

The loan may be prepaid at any time without premium or penalty. An initial disbursement of $2,000,000 ("Loan 1") was made to the Company on April 15, 2016 under the DECD Loan Agreement. On December 3, 2020, the Company received a disbursement of the remaining $2,000,000 ("Loan 2") under the DECD Loan Agreement, as the Company had achieved the target employment milestone necessary to receive an additional $1,000,000 under the DECD Loan Agreement and the DECD determined to fund the remaining $1,000,000 under the DECD Loan Agreement after concluding that the required revenue target would likely have been achieved in the first quarter of 2020 in the absence of the impacts of COVID-19.

Under the terms of the DECD Loan Agreement, the Company was eligible for forgiveness of up to $1,500,000 of the principal amount of the loan if it was able to achieve certain job creation and retention milestones by December 31, 2022. On June 26, 2023, the Company was notified by the DECD that the Company satisfied all job creation and retention requirements under the loan agreement to receive forgiveness of $1,000,000. If the Company failed to maintain its Connecticut operations through March 22, 2026, the DECD may have required early repayment of a portion or all of the loan plus a penalty of 5% of the total funded loan.

On January 30, 2024, the Company was granted a deferral of interest and principal payments on a portion of the remaining outstanding balances through June 1, 2024. The Company determined the loan deferrals met the definition of a troubled debt restructuring under ASC 470-60, *Troubled Debt Restructurings by Debtors*, as the Company was experiencing financial difficulties, and the lenders granted a concession. The future undiscounted cash flows of the DECD loan after the loan deferrals exceeded the carrying value of the DECD loan prior to the loan deferrals. As such no gain was recognized as a result of the deferrals.

On October 2, 2024, the Company executed an additional deferral agreement (the "October 2 Deferral"), which provided for both the interest and principal payments on Loan 1 to be deferred for August and September 2024. Payments resumed in October 2024. The October 2 Deferral also provides for both the interest and principal payments on Loan 2 to be deferred from August 2024 to May 2027, with payments resuming in June 2027. The Company determined these loan deferrals also met the definition of a troubled debt restructuring under ASC 470-60, *Troubled Debt Restructurings by Debtors*, as the Company was experiencing financial difficulties, and the lenders granted a concession. The future

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undiscounted cash flows of the DECD loan after the loan deferrals exceeded the carrying value of the DECD loan prior to the loan deferrals. As such, no gain was recognized as a result of the deferrals.

Long-term debt consisted of the following:

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| | | |
|:---|:---|:---|
| <br>**(in thousands)** | **December 31,** <br>**2025** | **December 31,** <br>**2024** |
| &nbsp;&nbsp;DECD loan, net of issuance costs | $1277 | $1507 |
| &nbsp;&nbsp;Less: Current portion, net of issuance costs | (236) | (229) |
| &nbsp;&nbsp;Total long-term debt, net of issuance costs | $1041 | $1278 |

---

As of December 31, 2025, the annual amounts of future minimum principal payments due under the Company's contractual obligation are shown in the table below. Unamortized debt issuance costs for the DECD loan were $1,000 as of December 31, 2025. Debt related to the insurance promissory note of $493,000, as described below, is not included in the following table due to the insurance promissory note being cancelable.

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| | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|
| | **Payments Due by Period** | **Payments Due by Period** | **Payments Due by Period** | **Payments Due by Period** | **Payments Due by Period** | **Payments Due by Period** | **Payments Due by Period** |
| <br>**(in thousands)** | **Total** | **2026** | **2027** | **2028** | **2029** | **2030** | **Thereafter** |
| DECD Loan | $1278 | $236 | $146 | $213 | $217 | $221 | $245 |
| &nbsp;&nbsp;Total | $1278 | $236 | $146 | $213 | $217 | $221 | $245 |

---

The DECD loan is classified within Level 3 of the fair value hierarchy. The following table presents the carrying value and fair value of the DECD loan. The fair value is estimated based on the discounted cash flows using the prevailing marketing interest rates.

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| | | | | | |
|:---|:---|:---|:---|:---|:---|
| | | **December 31,**  | **December 31,**  | **December 31,**  | **December 31,**  |
| | | **2025** | **2025** | **2024** | **2024** |
| <br>**(in thousands)** | <br>**Fair Value Hierarchy** | **Carrying Value** | **Fair Value** | **Carrying Value** | **Fair Value** |
| &nbsp;&nbsp;DECD loan | Level 3 | $1277 | $1038 | $1511 | $1169 |

---

The Company recorded interest expense of approximately $33,000 and $36,000 for the DECD loan for the years ended December 31, 2025 and 2024, respectively.

#### Insurance Notes
During 2025 and 2024, the Company entered into insurance promissory notes for the payment of insurance premiums at an interest rate of 8.24% and 7.52% respectively, with an aggregate principal amount outstanding of approximately $493,000 and $614,000 as of December 31, 2025 and 2024, respectively. Interest paid for the promissory notes was $21,000 and $23,000 for the years ended December 31, 2025 and 2024, respectively. The amount outstanding in 2025 could be substantially offset by the cancellation of the related insurance coverage which is classified in prepaid insurance. The 2025 notes are payable in eleven monthly installments with a maturity date of October 1, 2026. The 2024 notes were payable in nine monthly installments with a maturity date of August 1, 2025.

The carrying value of the Company's insurance promissory note approximates fair value at December 31, 2025 and 2024, due to the short-term nature of the insurance note and are classified as Level 2 within the fair value hierarchy.

#### Operating Leases
The Company leases facilities to support its business of discovering, developing and commercializing diagnostic tests in the fields of gynecologic disease. The Company's principal facility, including the CLIA laboratory used by Aspira Labs, is located in Austin, Texas, and an administrative office is also located in Shelton, Connecticut.

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In December 2024, the Company extended the Austin, Texas lease for an additional 54 months. The lease renewal also expanded the leased space. The Company's renewed lease expires on August 31, 2031, with the option to extend the lease for an additional three years. Variable lease costs represent our share of the landlord's operating expenses. The Company is not reasonably certain that it will exercise the three year renewal option beginning on September 1, 2031.

On May 30, 2023, the Company entered into a lease agreement for a facility in Shelton, Connecticut. The lease in Shelton, Connecticut cancelled and replaced the Trumbull, Connecticut office lease. The lease term is for five years, and its commencement date was October 1, 2023. Continuation of the lease after the lease term would be on a month-to-month basis.

In January 2023, the Company entered into a new sublease agreement for an administrative facility in Palo Alto, California. The Company's sublease term commenced in April 2023 and expired on May 31, 2024. The Company did not renew its lease with the sublessor. The sublessor, Invitae, filed for bankruptcy on February 15, 2024. The Company has applied for a refund of its approximately $10,000 security deposit with the bankruptcy court.

The expense associated with these operating leases for the years ended December 31, 2025 and 2024 is shown in the table below (in thousands). Included in the amounts below are $44,000 of short-term lease expenses related to rent for three short-term leases for the year ended December 31, 2025. There were no short term lease expenses for the year ended December 31, 2024.

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| | | | |
|:---|:---|:---|:---|
| | | **Year Ended December 31,**  | **Year Ended December 31,**  |
| <br>**Lease Cost** | <br>**Classification** | **2025** | **2024** |
| Operating rent expense |  |  |  |
|  | Cost of revenue | $146 | $87 |
|  | Research and development | 89 | 42 |
|  | Sales and marketing | 2 | 6 |
|  | General and administrative | 46 | 61 |
| Variable rent expense |  |  |  |
|  | Cost of revenue | $60 | $46 |
|  | Research and development | 34 | 11 |
|  | Sales and marketing | 2 | 7 |
|  | General and administrative | 52 | 35 |

---

Based on the Company's leases as of December 31, 2025, the table below sets forth the approximate future lease payments related to operating leases with initial terms of one year or more (in thousands).

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| | |
|:---|:---|
| **Year** | **Payments** |
| 2026 | $316 |
| 2027 | 283 |
| 2028 | 275 |
| 2029 | 230 |
| 2030 | 236 |
| Thereafter | 161 |
| Total Operating Lease Payments | 1501 |
| Less: Imputed Interest | (262) |
| Present Value of Lease Liabilities | 1239 |
| Less: Unamortized Tenant Improvement Allowance | (29) |
| Less: Operating Lease Liability, current portion | (202) |
| Operating Lease Liability, non-current portion | $1008 |

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Supplemental disclosure of cash flow information related to leases for the years ended December 31, 2025 and 2024 is shown in the table below (in thousands).

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| | | |
|:---|:---|:---|
|  | **Year Ended December 31,**  | **Year Ended December 31,**  |
|  | **2025** | **2024** |
| Cash paid for amounts included in measurement of lease liabilities: |  |  |
| &nbsp;&nbsp;Operating cash outflows relating to operating leases | $411 | $346 |
| Weighted-average remaining lease term (in years) | 5.1 | 5.9 |
| Weighted-average discount rate | 7.63% | 7.59% |

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#### Non-cancelable Royalty Obligations and Other Commitments
The Company is a party to an amended research collaboration agreement with The Johns Hopkins University School of Medicine under which the Company licenses certain of its intellectual property directed at the discovery and validation of biomarkers in human subjects, including but not limited to clinical application of biomarkers in the understanding, diagnosis and management of human disease. Under the terms of the amended research collaboration agreement, Aspira is required to pay the greater of 4% royalties on net sales of diagnostic tests using the assigned patents or annual minimum royalties of $57,500. Royalty expense for the years ended December 31, 2025 and 2024 totaled $289,000 and $293,000, respectively, and is recorded in cost of revenue in the Company's consolidated statements of operations.

#### Business Agreements
In August 2022, the Company entered into a sponsored research agreement with Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz (the "Dana-Farber, Brigham, Lodz Research Agreement"), for the generation of a multi-omic, non-invasive diagnostic aid to identify endometriosis based on circulating microRNAs and proteins. The Dana-Farber, Brigham, Lodz Research Agreement required payments to be made upon the achievement of certain milestones. Under the terms of and as further described in the Dana-Farber, Brigham, Lodz Research Agreement, payments of approximately $1,252,000 were due from the Company to the counterparties upon successful completion of certain deliverables as follows: 68% was paid in 2022, 15% was paid in 2023, and the remaining 17% was paid in 2025. During the year ended December 31, 2025, approximately $50,000 was recorded as research and development expense in the Company's consolidated financial statement of operations for the project. During the year ended December 31, 2024, no expense was recorded as research and development expense in the Company's consolidated financial statement of operations for the project. From the inception of the Dana-Faber, Brigham, Lodz Research Agreement through December 31, 2025, research and development expenses in the cumulative amount of $1,252,000 have been recorded and paid.

On March 20, 2023, the Company entered into a licensing agreement ("Dana-Faber, Brigham, Lodz License Agreement") with Harvard's Dana-Farber Cancer Institute, Brigham & Women's Hospital, and Medical University of Lodz under which the Company will license certain of its intellectual property to be used in the Company's OvaSuite product portfolio. Under the Dana-Faber, Brigham, Lodz License Agreement, the Company paid an initial license fee of $75,000, which was recorded as research and development expense on the Company's consolidated financial statement of operations, and pays an annual license maintenance fee of $50,000 on each anniversary of the date of the Dana-Faber, Brigham, Lodz License Agreement. The Dana-Faber, Brigham, Lodz License Agreement also requires non-refundable royalty payments of up to $1,350,000 based on certain regulatory approvals and commercialization milestones and further royalty payments based on the net sales of the Company's products included under the Dana-Faber, Brigham, Lodz License Agreement. No milestones have been reached as of December 31, 2025.

#### Contingent Liabilities
From time to time, the Company is involved in legal proceedings and regulatory proceedings arising from operations. The Company establishes reserves for specific liabilities in connection with legal actions that management deems to be probable and estimable. The Company is not currently a party to any proceeding, the adverse outcome of which would have a material adverse effect on the Company's financial position or results of operations.

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#### NOTE 8: COMMON STOCK

#### 2023 Equity Line of Credit
On March 28, 2023, the Company entered into a purchase agreement (the "2023 Equity Line of Credit Agreement") with Lincoln Park Capital Fund, LLC ("Lincoln Park") and a registration rights agreement (the "LPC Registration Rights Agreement"), pursuant to which the Company has the right, in its sole discretion, to sell to Lincoln Park shares of the Company's common stock, par value $0.001 per share (the "Common Stock"), having an aggregate value of up to $10,000,000 (the "Purchase Shares"), subject to certain limitations and conditions set forth in the 2023 Equity Line of Credit Agreement. The Company will control the timing and amount of any sales of Purchase Shares to Lincoln Park pursuant to the 2023 Equity Line of Credit Agreement.

During the year ended December 31, 2025, no shares were sold under the 2023 Equity Line of Credit Agreement. During the year ended December 31, 2024, the Company sold 949,574 shares under the 2023 Equity Line of Credit Agreement for gross proceeds of approximately $1,900,000. Over the life of the 2023 Equity Line of Credit Agreement through December 31, 2025, the Company sold 1,310,517 shares for gross proceeds of approximately $3,078,000. The Company incurred approximately $326,000 of costs related to the execution of the 2023 Equity Line of Credit Agreement, all of which are reflected in the consolidated financial statements. The Company incurred approximately $0 and $249,000 for legal fees during the year ended December 31, 2025 and 2024, respectively, and included the costs in general and administrative expenses on its consolidated statement of operations. Under the terms of a Warrant Inducement Agreement, the Company agreed not to sell shares under the 2023 Equity Line of Credit Agreement for six months from the effective date of the Form S-3, which was September 3, 2024. As of March 30, 2026, the remaining availability under the 2023 Equity Line of Credit Agreement was $1,700,000 of shares of Common Stock that can be sold to Lincoln Park under the 2023 Equity Line of Credit Agreement; however, as the Company is no longer traded on Nasdaq, it does not have access to this facility.

#### 2024 Registered Direct Offering
On January 24, 2024, the Company entered into a securities purchase agreement (the "2024 Direct Offering Agreement"), with several investors relating to the issuance and sale of 1,371,000 shares of its common stock, par value $0.001 per share, and pre-funded warrants to purchase 200,000 shares of Common Stock (the "Pre-Funded Warrants"), in a registered direct offering, together with accompanying warrants to purchase 1,571,000 shares of Common Stock (the "January 2024 Purchase Warrants", and together with the Pre-Funded Warrants, the "January 2024 Warrants") in a concurrent private placement (the "Concurrent Private Offering" and together with the registered direct offering, the "2024 Direct Offering").

Pursuant to the 2024 Direct Offering Agreement, the Company issued 1,368,600 shares of common stock to certain investors at an offering price of $3.50 per share, and 2,400 shares of common stock to an executive officer, at an offering price of $4.255 per share, which was the consolidated closing bid price of the Company's common stock on The Nasdaq Capital Market on January 24, 2024 of $4.13 per share plus $0.125 per January 2024 Purchase Warrant. The purchase price of each Pre-Funded Warrant is equal to the combined purchase price at which a share of Common Stock and the accompanying January 2024 Purchase Warrant is sold in this 2024 Direct Offering, minus $0.0001. The gross proceeds to the Company from the 2024 Direct Offering were approximately $5,563,000, before deducting placement agent fees and other expenses of approximately $733,000 payable by the Company. The 2024 Direct Offering closed on January 26, 2024.

The Pre-Funded Warrants were exercisable at any time after the date of issuance and had an exercise price of $0.0001 per share. A holder of Pre-Funded Warrants could not exercise the warrant if the holder, together with its affiliates, would beneficially own more than 9.99% of the number of shares of Common Stock outstanding immediately after giving effect to such exercise. A holder of Pre-Funded Warrants may increase or decrease this percentage to a percentage not in excess of 9.99% by providing at least 61 days' prior notice to the Company. All of the Pre-Funded Warrants were exercised on February 6, 2024 for gross proceeds of $20.

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The January 2024 Purchase Warrants have an exercise price of $4.13 per share and were exercisable beginning six months after issuance. 1,400,000 of the January 2024 Purchase Warrants were exercised on August 1, 2024 under a Warrant Inducement Agreement at a reduced price of $1.25 per share.

The Company engaged AGP to act as sole placement agent in the 2024 Direct Offering. The Company paid the placement agent a cash fee equal to 7.0% of the aggregate gross proceeds generated from the 2024 Direct Offering, except that, with respect to proceeds raised in this 2024 Direct Offering from certain designated persons, AGP's cash fee is reduced to 3.5% of such proceeds, and to reimburse certain fees and expenses of the placement agent in connection with the 2024 Direct Offering. The Company also reimbursed the placement agent for its accountable offering-related legal expenses of $75,000 and a non-accountable expense allowance of $30,000. Costs related to the 2024 Direct Offering were recorded as an offset to additional paid-in capital on the Company's balance sheet as of December 31, 2024.

The Company evaluated the Pre-Funded Warrants and the January 2024 Purchase Warrants and concluded that they met the criteria to be classified as equity within additional paid-in-capital.

The Pre-Funded Warrants are equity classified because they (1) are freestanding financial instruments that are legally detachable and separately exercisable from the common stock, (2) are immediately exercisable, (3) do not embody an obligation for the Company to repurchase its shares, (4) permit the holder to receive a fixed number of shares of common stock upon exercise, (5) are indexed to the Company's common stock and (6) meet the equity classification criteria.

The January 2024 Purchase Warrants are equity classified because they (1) are freestanding financial instruments that are legally detachable and separately exercisable from the common stock, (2) do not embody an obligation for the Company to repurchase its shares, (3) permit the holder to receive a fixed number of shares of common stock upon exercise, (4) are indexed to the Company's common stock and (5) meet the equity classification criteria.

Effective upon the closing of the 2024 Direct Offering, the Company also amended certain existing warrants (the "August 2022 Warrants") to purchase up to an aggregate of 366,664 shares at an exercise price of $13.20 per share and a termination date of August 25, 2027, so that the amended August 2022 Warrants have a reduced exercise price of $4.13 per share and a new termination date of January 26, 2029. The other terms of the amended August 2022 Warrants remain unchanged. The Company performed an analysis of the fair value of the August 2022 Warrants immediately before and after the modification and the increase in fair value of the August 2022 Warrants of $490,000 was recorded as a change in fair value of warrant liabilities in the Company's consolidated statement of operations.

Approximately $106,000 of the costs related to the 2024 Direct Offering were allocated to the August 2022 Warrants and were recorded as other expense in the Company's consolidated statement of operations.

#### 2024 Private Placement Offering
On July 1, 2024, the Company entered into a securities purchase agreement with certain investors in a private placement (the "2024 Private Placement Offering"). Pursuant to the 2024 Private Placement Offering, the Company issued an aggregate of 1,248,529 shares of its common stock and accompanying warrants (the "July 2024 Purchase Warrants") to purchase an equal number of shares of common stock at a price of $1.53 per share and accompanying warrant. The July 2024 Purchase Warrants have an exercise price of $2.25 per share and are exercisable until their expiration on the third anniversary of the issuance date. The gross proceeds to the Company from the 2024 Private Placement Offering were approximately $1,909,000, before deducting expenses of approximately $72,000 payable by the Company.

The Company evaluated the July 2024 Purchase Warrants and concluded that they met the criteria to be classified as equity within additional paid-in-capital.

The July 2024 Purchase Warrants are equity classified because they (1) are freestanding financial instruments that are legally detachable and separately exercisable from the common stock, (2) do not embody an obligation for the Company to repurchase its shares, (3) permit the holder to receive a fixed number of shares of common stock upon exercise, (4) are indexed to the Company's common stock and (5) meet the equity classification criteria.

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In February 2025, certain July 2024 Purchase Warrants were modified to require shareholder approval of the July 2024 Purchase Warrants prior to their becoming exercisable.

#### 2024 At the Market Offering
On August 2, 2024, the Company entered into an agreement with H.C. Wainwright in connection with an At the Market offering agreement (the "2024 At the Market Offering") to sell shares of its common stock ("Common Stock"), having an aggregate sales price of up to $4,450,000, from time to time, through an "at the market offering" program under which H.C. Wainwright acts as sales agent. The Company pays Wainwright a commission rate equal to 3.0% of the aggregate gross proceeds from each sale of shares under the 2024 At the Market Offering. The Company has also reimbursed H.C. Wainwright for certain specified expenses in connection with entering into the 2024 At the Market Offering.

During the year ended December 31, 2024, the Company sold 1,073,050 shares under the 2024 At the Market Offering for gross proceeds of approximately $903,000. The Company incurred approximately $240,000 of costs related to the execution of the 2024 At the Market Offering, all of which were recorded as an offset to additional paid-in capital on the Company's balance sheet as of December 31, 2024.

During the year ended December 31, 2025, the Company sold 12,277,441 shares under the 2024 At the Market Offering Agreement for gross proceeds of approximately $3,484,000 before deducting expenses of approximately $147,000. The total gross proceeds to the Company over the life of the At the Market Offering Agreement is $4,388,000 and the value of the remaining availability was approximately $62,000 that could be sold to H.C. Wainwright under the 2024 At the Market Offering Agreement, subject to the terms of the 2024 At the Market Offering Agreement.

#### 2024 Securities Purchase Agreements
In August 2024, the Company entered into securities purchase agreements with two shareholders under which it sold a total of 9,733 shares of common stock and received proceeds of approximately $11,000.

#### 2024 Warrant Inducement Agreement
On July 31, 2024, the Company entered into a warrant inducement agreement (the "Warrant Inducement Agreement") with a certain holder (the "Holder") of (i) warrants to purchase 311,111 shares of Common Stock dated August 22, 2022 (the "August 2022 Warrants") and (ii) warrants to purchase 1,400,000 shares of Common Stock dated January 26, 2024 (the "January 2024 Warrants"), pursuant to which the Holder agreed to exercise in cash the warrants held at a reduced exercise price of $1.25 per share (reduced from $4.13 per share for the August 2022 Warrants and $4.13 for the January 2024 Warrants).

As an inducement to such exercise, the Company agreed to issue to the Holder new Common Stock warrants (collectively, the "August 2024 Purchase Warrants"), to purchase up to 2,566,667 shares of Common Stock. The August 2024 Purchase Warrants were exercisable immediately after issuance and will expire 5 years from the initial exercise date.

The transaction, which closed on August 1, 2024, resulted in net proceeds of approximately $1,862,000. The Warrant Inducement Agreement was entered into to encourage the exercise of the August 2022 Warrants and January 2024 Purchase Warrants in order to obtain capital for operations. The $1,323,000 incremental value transferred for the modification to both the August 2022 Warrants and January 2024 Purchase Warrants as a result of the Warrant Inducement Amendment was accounted for as an equity issuance cost and recognized within additional paid in capital in the audited consolidated balance sheets.

The Company evaluated the August 2024 Purchase Warrants and concluded that they met the criteria to be classified as equity within additional paid-in-capital.

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The August 2024 Purchase Warrants are equity classified because they (1) are freestanding financial instruments that are legally detachable and separately exercisable from the common stock, (2) do not embody an obligation for the Company to repurchase its shares, (3) permit the holder to receive a fixed number of shares of common stock upon exercise, (4) are indexed to the Company's common stock and (5) meet the equity classification criteria.

Under the terms of the Warrant Inducement Agreement, the Company was prohibited from selling shares under the 2024 At the Market Offering until September 30, 2024. It was also prohibited from selling shares under our 2023 equity line of credit for a period of six months from the effective date of the Form S-3, which was September 3, 2024.

#### March 2025 Convertible Notes Settlement
On March 12, 2025, the Company issued the March 2025 Warrants upon the conversion of the March 2025 Convertible Notes. The March 2025 Warrants were exercisable for five years at $0.25 per share for the first 24 months after issuance, and $0.50 per share thereafter. In September 2025, the March 2025 Warrants were modified to a single exercise price of $0.35 per share and the termination date was extended to March 5, 2031.

The March 2025 Warrants are classified in warrant liabilities on the Company's consolidated balance sheets. The March 2025 Warrants are classified as liabilities because the settlement amount is not indexed to the Company's stock as required by ASC 815, *Derivatives and Hedging*.

#### 2025 Equity Purchase Agreement
On April 4, 2025, the Company entered into an equity purchase agreement (the "2025 Equity Purchase Agreement") with Triton Funds L.P. ("Triton") for the purchase of up to $2,000,000 of the Company's shares of common stock.

Pursuant to the 2025 Equity Purchase Agreement, the Company issued 354,988 shares of restricted common stock to Triton for $25,000 and upon effectiveness of a registration statement registering the shares of Common Stock, the Company will be able to close on the sale of up to $2,000,000 of Common Stock. The purchase price of the Common Stock is 75% of the lowest traded price of the Common Stock five (5) business days prior to a closing. Transaction costs of approximately $214,000 related to the execution of the 2025 Equity Purchase Agreement were incurred, all of which are recognized in Other expense (income), net in the consolidated financial statements.

#### 2025 Private Placement Offering
**On September 16, 2025, the Company entered into a securities purchase agreement with certain investors and board members in a private placement (the "2025 Private Placement Offering"). Pursuant to the 2025 Private Placement Offering, the Company issued an aggregate of 6,550,000 shares of its common stock and accompanying warrants (the "September 2025 Warrants") to purchase an equal number of shares of common stock at a price of $0.45 per share and accompanying warrant. The September 2025 Warrants have an exercise price of $0.75 per share and are exercisable until their expiration on the fifth anniversary of the issuance date. The gross proceeds to the Company from the 2025 Private Placement Offering were approximately $2,950,000, before deducting issuance costs of approximately $140,000.**

#### The Company evaluated the September 2025 Warrants and concluded that they met the criteria to be classified as equity within additional paid-in-capital.

#### 2025 Lincoln Park Equity Line of Credit Agreement
On December 23, 2025, the Company entered into an equity purchase agreement (the "2025 Lincoln Park Agreement") with Lincoln Park Capital Fund, LLC ("Lincoln Park"), pursuant to which Lincoln Park committed to purchase, at the Company's direction from time to time, up to an aggregate of $10,000,000 of the Company's Common Stock. In connection therewith, the Company also entered into a registration rights agreement with Lincoln Park (the "Registration Rights Agreement" and, together with the 2025 Lincoln Park Agreement, the "Lincoln Park Agreements"), pursuant to which the Company agreed to file with the U.S. Securities and Exchange Commission (the "SEC") a

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registration statement covering the resale by Lincoln Park of the shares of Common Stock that have been and may be issued and sold to Lincoln Park under the 2025 Lincoln Park Agreement, including the commitment shares described below, and to take such other actions as are reasonably necessary to maintain the effectiveness of such registration statement as provided in the Registration Rights Agreement.

Under the terms of the 2025 Lincoln Park Agreement, from and after the date on which the conditions to Lincoln Park's purchase obligations have been satisfied, including that the registration statement described above is declared effective by the SEC and a final prospectus is filed with the SEC (the "Commencement Date"), the Company will have the right, but not the obligation, in its sole discretion to direct Lincoln Park to purchase shares of Common Stock from time to time over a period of up to 24 months, for aggregate gross proceeds to the Company of up to $10,000,000. Lincoln Park has no right to require the Company to sell any shares of Common Stock, but Lincoln Park is obligated to make purchases of Common Stock from the Company as directed by the Company in accordance with the 2025 Lincoln Park Agreement.

From and after the Commencement Date, on any business day on which the closing sale price of the Common Stock is greater than $0.10 per share, the Company may, by written notice, direct Lincoln Park to purchase up to 50,000 shares of Common Stock (a "Regular Purchase"), which amount may be increased to up to 75,000 shares if the closing sale price is not below $0.50 per share and up to 100,000 shares if the closing sale price is not below $0.75 per share, in each case subject to a maximum dollar amount of $500,000 per Regular Purchase. The purchase price per share for each Regular Purchase will be equal to 95% of the lower of (i) the lowest sale price of the Common Stock on the applicable purchase date and (ii) the average of the three lowest closing sale prices of the Common Stock during the ten business days immediately preceding the applicable purchase date. Regular Purchases may be effected as frequently as each business day after the close of trading so that the applicable purchase price is fixed and known at the time the Company elects to sell shares to Lincoln Park.

In addition, if the Company directs Lincoln Park to purchase the maximum number of shares permitted in a Regular Purchase on an applicable purchase date, then, in addition to such Regular Purchase and subject to the satisfaction of certain conditions and limitations set forth in the Purchase Agreement, the Company may also direct Lincoln Park to purchase additional shares of Common Stock in one or more accelerated purchases (each, an "Accelerated Purchase") on the following business day. The Company may set a minimum price threshold for any Accelerated Purchase in the related notice. For any Accelerated Purchase, Lincoln Park will purchase the lesser of (i) three times the number of shares purchased in the corresponding Regular Purchase and (ii) 30% of the trading volume on the Accelerated Purchase date, at a purchase price per share equal to the lower of 95% of (x) the closing sale price on the Accelerated Purchase date and (y) the volume-weighted average price for such date. Subject to satisfaction of the applicable conditions, the Company may direct multiple Accelerated Purchases in a single trading day, provided share deliveries for prior purchases have been completed.

The 2025 Lincoln Park Agreement contains customary terms, conditions, representations and warranties, and indemnification obligations of the parties. The Company may terminate the 2025 Lincoln Park Agreement at any time, for any reason or no reason, upon one business day's prior written notice to Lincoln Park, at no cost or penalty. Following the Commencement Date, upon the occurrence of specified suspension events described in the 2025 Lincoln Park Agreement, including, among others, the unavailability of the registration statement for resales, trading suspensions, certain breaches of representations or covenants having or reasonably likely to have a material adverse effect, and certain listing or eligibility events, the Company will not be permitted to direct Lincoln Park to purchase shares until the applicable suspension event is cured or waived; provided that Lincoln Park does not have the right to terminate the 2025 Lincoln Park Agreement as a result of any such suspension event. In addition, the 2025 Lincoln Park Agreement prohibits the Company from directing Lincoln Park to purchase any shares of Common Stock if such shares, when aggregated with all other shares then beneficially owned by Lincoln Park and its affiliates, would result in Lincoln Park beneficially owning more than 4.99% of the outstanding shares of Common Stock, which beneficial ownership cap may be increased by Lincoln Park to up to 9.99% upon 61 days' prior written notice to the Company.

As consideration for Lincoln Park's commitment to purchase shares under the 2025 Lincoln Park Agreement, on the date of the 2025 Lincoln Park Agreement the Company issued to Lincoln Park 824,493 shares of Common Stock (the "Commitment Shares"). Transaction costs of approximately $412,000 related to the execution of the 2025 Lincoln Park

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Agreement were incurred, all of which are recognized in Other expense (income), net in the consolidated financial statements. Also included in Other expense (income), net, was a referral fee related to the 2025 Lincoln Park Agreement in the amount of $84,000. In addition, the Company incurred approximately $85,000 for legal fees and $28,000 related to the 2025 Lincoln Park Agreement during the year ended December 31, 2025, and included the costs in general and administrative expenses on its consolidated statement of operations.

The 2025 Lincoln Park Agreement prohibits the Company from entering into another equity line of credit or substantially similar arrangement during the 24-month term of the 2025 Lincoln Park Agreement; however, the Company may enter into or maintain an at-the-market offering program with a registered broker-dealer.

For additional information, see Note 13, *Subsequent Events*.

***Warrants***

Certain of the Company's Warrants are classified as a long-term Warrant liability on the Company's balance sheet. The fair value of the Warrants was estimated using Black-Scholes pricing model based on the following assumptions:

---

| | | | | | |
|:---|:---|:---|:---|:---|:---|
|  | **December 31,**  | **December 31,**  | **December 31,**  | **December 31,**  | **December 31,**  |
|  | **2025** | **2025** | **2025** | **2024** | **2024** |
|  | **Amended March 2025 Warrants** | **Unmodified August 2022 Warrants** | **Modified August 2022 Warrants** | **Unmodified August 2022 Warrants** | **Modified August 2022 Warrants** |
| Dividend yield | —% | —% | —% | —% | —% |
| Volatility | 130.0% | 175.9% | 147.3% | 111.3% | 103.6% |
| Risk-free interest rate | 3.73% | 3.48% | 3.55% | 4.22% | 4.28% |
| Expected lives (years) | 5.18 | 1.64 | 3.07 | 2.64 | 4.07 |
| Weighted average fair value | $0.572 | $0.176 | $0.369 | $0.101 | $0.162 |

---

The fair value of the August 2022 Warrants, which were deemed to be derivative instruments due to the certain contingent put feature, was determined using the Black-Scholes option pricing model. The Black-Scholes option pricing model was deemed to be an appropriate model due to the terms of the Warrants issued, including a fixed term and exercise price.

The fair value of the August 2022 Warrants was affected by changes in inputs to the Black-Scholes option pricing model including the Company's stock price, expected stock price volatility, the contractual term, and the risk-free interest rate. This model uses Level 2 inputs, including stock price volatility, in the fair value hierarchy established by ASC 820 Fair Value Measurement.

The fair values of the August 2022 Warrants as of December 31, 2025 and December 31, 2024 were $97,000 and $61,000, respectively.

The Company recorded warrants in connection with the March conversion of Senior Secured Convertible Promissory Notes (the "Convertible Notes") in 2025 (the "March 2025 Warrants") in warrant liabilities. The March 2025 Warrants were deemed to be derivative instruments due to certain contingent put features. As discussed in Note 8, *Common Stock*, in connection with the conversion of convertible notes in March 2025, the Company issued warrants to purchase up to an aggregate of 12,298,177 shares of common stock. The March 2025 Warrants had an exercise price of $0.25 per share for the first 24 months after issuance, and $0.50 per share thereafter, but were modified in September 2025 to an exercise price of $0.35 per share (the "Amended March 2025 Warrants"), utilizing a fixed expiration date.

The fair value of the March 2025 Warrants as of December 31, 2025 and March 12, 2025 was approximately $6,777,000 and $1,477,000, respectively. The fair value of the March 2025 Warrants was estimated using a Monte Carlo simulation pricing model for its March 12, 2025 fair value assessment, which uses Level 3 inputs due to its incorporation

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of significant inputs that are not observable in the market. The mean present value across multiple simulation iterations was used to estimate the fair value of the March 2025 Warrants.

Following the modification, the fair value of the Amended March 2025 Warrants was determined using the Black-Scholes option pricing model, deemed to be an appropriate model due to the terms of the Amended March 2025 Warrants issued, including a fixed term and exercise price.

The following table presents the Company's transfers from Level 3 to Level 2 for the year ended December 31, 2025. There were no transfers for the year ended December 31, 2024.

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| | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|
| (Amounts in Thousands) | Balance as of January 1, 2025 | Issuance of Warrants | Change in Far Value through Date of Modification | Exercises | Transfer out to Level 2 | Balance as of December 31, 2025 |
| Level 3 Warrant Liabilities | $- | $1477 | $3414 | $(270) | $(4621) | $- |

---

Warrants outstanding as of December 31, 2025 and 2024 were as follows:

---

| | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|
|  | | |  | | **Number of Warrants** | **Number of Warrants** |
|  | | |  | | **Outstanding and Common** | **Outstanding and Common** |
|  | | |  | | **Stock Underlying Warrants** | **Stock Underlying Warrants** |
|  | | |  | | **December 31,**  | **December 31,**  |
|  | <br>**Issuance Date** | <br>**Expiration Date** | **Classification** | <br>**Exercise**<br>**Price**<br>**per Share** | **2025** | **2024** |
| Unmodified August 2022 Warrants | August 25, 2022 | August 25, 2027 | Liability | $13.20 | 433321 | 433321 |
| Modified August 2022 Warrants | August 25, 2022 | August 25, 2027 | Liability | $4.13 | 55553 | 55553 |
| January 2024 Purchase Warrants | January 26, 2024 | July 26, 2029 | Equity | $4.13 | 171000 | 171000 |
| July 2024 Purchase Warrants | July 9, 2024 | July 9, 2027 | Equity | $2.25 | 1248527 | 1248527 |
| August 2024 Purchase Warrants | August 1, 2024 | August 1, 2029 | Equity | $1.36 | 2566667 | 2566667 |
| Amended March 2025 Warrants | September 19, 2025 | March 5, 2031 | Liability | $0.35 | 11848177 | - |
| September 2025 Warrants | September 19, 2025 | September 19, 2031 | Equity | $0.75 | 4912500 | - |
|  |  |  |  |  | 21235745 | 4475068 |

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&nbsp;&nbsp;&nbsp;&nbsp;(1) Liability classified

&nbsp;&nbsp;&nbsp;&nbsp;(2) Equity classified

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#### NOTE 9: LOSS PER SHARE
The Company calculates basic loss per share using the weighted average number of shares of Aspira common stock outstanding during the period. The Company considers the August 2022 Warrants, the January 2024 Purchase Warrants, the July 2024 Purchase Warrants, the August 2024 Purchase Warrants, the March 2025 Warrants and the September 2025 Warrants to be participating securities, because holders of such instruments participate in the event a dividend is paid on common stock. The holders of the August 2022 Warrants, the January 2024 Purchase Warrants, the July 2024 Purchase Warrants, the August 2024 Purchase Warrants, the March 2025 Warrants and the September 2025 Warrants do not have a contractual obligation to share in the Company's losses. As such, losses are attributed entirely to common stockholders and for periods in which the Company has reported a net loss, diluted loss per common share is the same as basic loss per common share.

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| | | |
|:---|:---|:---|
|  | **Year Ended**  | **Year Ended**  |
|  | **December 31,**  | **December 31,**  |
|  | **2025** | **2024** |
| Numerator: |  |  |
| &nbsp;&nbsp;Net Loss | $(12780) | $(13094) |
| Denominator: |  |  |
| &nbsp;&nbsp;Shares used in computing net loss per share, basic and diluted | 34102760 | 14134626 |
| Net loss per share, basic and diluted | $(0.37) | $(0.93) |

---

Due to net losses for the years ended December 31, 2025 and 2024, diluted loss per share is calculated using the weighted average number of common shares outstanding and excludes the effects of potential shares of common stock that are antidilutive.

The potential shares of common stock that have been excluded from the diluted loss per share calculation above for the years ended December 31, 2025 and 2024 were as follows:

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| | | |
|:---|:---|:---|
|  | **Year Ended December 31,**  | **Year Ended December 31,**  |
|  | **2025** | **2024** |
| Stock options | 3,114,229 | 876,249 |
| Restricted stock units | - | 149,061 |
| Warrants | 21,235,745 | 4,475,068 |
| &nbsp;&nbsp;Potential common shares | 24,349,974 | 5,500,378 |

---

#### NOTE 10: EMPLOYEE SHARE BASED COMPENSATION AND BENEFIT PLANS

#### 2010 Stock Incentive Plan
The Company's employees, directors, and consultants were eligible to receive awards under the Vermillion, Inc. Second Amended and Restated 2010 Stock Incentive Plan, which was replaced by the 2019 Plan (as defined below) with respect to future equity grants. As of December 31, 2025, there were no shares of Aspira common stock available for future grants under the 2010 Plan.

As of December 31, 2025, a total of 13,442 shares were reserved for issuance with respect to outstanding stock options.

#### 2019 Stock Incentive Plan
At the Company's 2019 annual meeting of stockholders, the Company's stockholders approved the Vermillion, Inc. 2019 Stock Incentive Plan, which was later amended to the Aspira Women's Health Inc. (the "2019 Plan"). The purposes of the 2019 Plan are (i) to align the interests of the Company's stockholders and recipients of awards under the 2019 Plan by increasing the proprietary interest of such recipients in the Company's growth and success; (ii) to advance

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the interests of the Company by attracting and retaining non-employee directors, officers, other employees, consultants, independent contractors and agents; and (iii) to motivate such persons to act in the long-term best interests of the Company and its stockholders. The 2019 Plan allows the Company to grant stock options, stock appreciation rights, restricted stock, restricted stock units and performance awards to participants.

Subject to the terms and conditions of the 2019 Plan, the initial number of shares authorized for grants under the 2019 Plan was 699,485. With shareholder approval, the total number of shares available to be issued as of December 31, 2025 has been increased to 4,532,818 shares. To the extent an equity award granted under the 2019 Plan expires or otherwise terminates without having been exercised or paid in full, or is settled in cash, the shares of common stock subject to such award will become available for future grant under the 2019 Plan. As of December 31, 2025, there were 790,376 shares of Aspira common stock available for future grants under the 2019 Plan.

The stock option activity under the 2019 Plan for the years ended December 31, 2025 and 2024 was as follows:

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| | | | | |
|:---|:---|:---|:---|:---|
|  | <br>**Number**<br>**of Shares** | <br>**Weighted**<br>**Average**<br>**Exercise**<br>**Price** | <br>**Aggregate**<br>**Intrinsic**<br>**Value** | **Weighted**<br>**Average**<br>**Remaining**<br>**Contractual**<br>**Term** |
| Options outstanding at December 31, 2024 | 861342 | 6.11 | $- | 6.89 |
| &nbsp;&nbsp;Options granted | 2912131 | 0.27 |  |  |
| &nbsp;&nbsp;Options forfeited or expired | (672686) | 5.19 |  |  |
| Options outstanding at December 31, 2025 | 3100787 | $6.11 | $1020811 | 9.54 |
| Vested and Exercisable options at December 31, 2025 | 1499581 | $1.20 | $375726 | 9.54 |

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&nbsp;&nbsp;&nbsp;&nbsp;(1) Options outstanding include options vested and expected to vest

There were no options exercised for the years ended December 31, 2025 and 2024.

For the year ended December 31, 2025, the Company granted option awards under the 2019 Plan with a weighted average grant date fair value of $0.20.

Assumptions included in the fair value per share calculations during the year ended December 31, 2025, were (i) expected terms of two to three years, (ii) two to three year treasury interest rates of 3.51% to 4.25% and (iii) market close prices ranging from $0.07 to $0.72. The Company recorded $79,000 in forfeitures for the year ended December 31, 2025.

For the year ended December 31, 2024, the Company granted option awards under the 2019 Plan with a weighted average grant date fair value of $1.36.

Assumptions included in the fair value per share calculations for the year ended December 31, 2024, were (i) expected terms of one to three years, (ii) one to three year treasury interest rates of 3.58% to 4.96% and (iii) market close prices ranging from $0.75 to $4.87. The Company recorded $13,000 in forfeitures for the year ended December 31, 2024.

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The following table summarizes RSU activity for the 2019 Plan for the years ended December 31, 2025 and 2024.

---

| | | |
|:---|:---|:---|
|  | <br>**Number of**<br>**Shares** | **Weighted Average** <br>**Grant Date Fair** <br>**Value per Share** |
| RSUs outstanding at December 31, 2024 | 149061 | $1.02 |
| &nbsp;&nbsp;RSUs granted | 6458 | 0.14 |
| &nbsp;&nbsp;RSUs vested and issued | (150519) | 0.98 |
| &nbsp;&nbsp;RSUs forfeited or expired | (5000) | 1.06 |
| RSUs outstanding at December 31, 2025 | - |  |

---

During the years ended December 31, 2025 and 2024, the Company granted RSUs with a weighted average grant date fair value of $152,000 and $360,000, respectively. The total fair value of RSUs outstanding was $0 and $326,000 for the years ended December 31, 2025 and 2024, respectively.

#### Stock-based Compensation
The allocation of non-cash stock-based compensation expense by functional area for the years ended December 31, 2025 and 2024 was as follows.

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| | | |
|:---|:---|:---|
| | **Year Ended**  | **Year Ended**  |
| | **December 31,**  | **December 31,**  |
| <br>**(in thousands)** | **2025** | **2024** |
| Cost of revenue | $18 | $36 |
| Research and development | 27 | 195 |
| Sales and marketing | 45 | 153 |
| General and administrative | 441 | 1110 |
| Total | $531 | $1494 |

---

As of December 31, 2025, total unrecognized compensation cost related to unvested stock option awards was approximately $289,000, and the related weighted average period over which it is expected to be recognized was 1.21 years. As of December 31, 2024, there was $294,000 in unrecognized compensation costs related to restricted stock units, and the related weighted average period over which it is expected to be recognized is 1.85 years.

#### 401(k) Plan
The Company's 401(k) Plan allows eligible employees to defer up to an annual limit of the lesser of 90.0% of eligible compensation or a maximum contribution amount subject to the Internal Revenue Service annual contribution limit. The Company is not required to make Company contributions under the 401(k) Plan. For the years ended December 31, 2025 and 2024, the Company did not make Company contributions to the 401(k) Plan.

#### NOTE 11: INCOME TAXES
There was no current income tax expense or benefit for the years ended December 31, 2025 or 2024 because of net losses during those years. These net losses were generated from domestic operations. Loss from continuing operations before income taxes for the years ended December 31, 2025 and 2024 were $12,780,000 and $13,094,000, respectively.

Based on the available objective evidence, management believes it is more likely than not that the net deferred tax assets will not be fully realizable. Accordingly, the Company has provided a full valuation allowance against its net deferred tax assets at December 31, 2025 and 2024. Therefore, there was no deferred income tax expense or benefit for the years ended December 31, 2025 or 2024.

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The components of net deferred tax assets at December 31, 2025 and 2024 were as follows:

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| | | |
|:---|:---|:---|
| | **Year Ended December 31,**  | **Year Ended December 31,**  |
| <br>**(in thousands)** | **2025** | **2024** |
| Deferred tax assets: |  |  |
| &nbsp;&nbsp;Net operating losses | $31076 | $55899 |
| &nbsp;&nbsp;Capitalized research expenses | 2298 | 3120 |
| &nbsp;&nbsp;Fixed asset depreciation | 427 | 477 |
| &nbsp;&nbsp;Other | 457 | 585 |
| &nbsp;&nbsp;ASC 842 Right of Use Liability | 286 | 300 |
| Total deferred tax assets | 34544 | 60381 |
| Valuation allowance | (34311) | (60096) |
| &nbsp;&nbsp;Deferred tax assets | $233 | $285 |
| Deferred tax liabilities: |  |  |
| &nbsp;&nbsp;ASC 842 Right of Use Asset | $(233) | $(285) |
| &nbsp;&nbsp;&nbsp;&nbsp;Deferred tax liabilities | $(233) | $(285) |
| &nbsp;&nbsp;&nbsp;&nbsp;Net deferred tax assets | $- | $- |

---

Due to uncertainties surrounding the Company's ability to generate future taxable income to realize these assets, a full valuation allowance has been established to offset the net deferred tax assets. In assessing the realization of deferred tax assets, management considered whether it was more likely than not that some, or all, of the deferred tax assets will be realized. The ultimate realization of the deferred tax assets is dependent upon the generation of future taxable income. Management has considered the history of the Company's operating losses and believes that the realization of the benefit of the deferred tax assets cannot be reasonably assured.

Pursuant to Section 382 of the Internal Revenue Code, (the "IRC"), annual use of the Company's net operating loss ("NOL") carryforwards may be limited in the event a cumulative change in ownership of more than 50% occurs within a three-year period. Such ownership change could result in annual limitations on the utilization of tax attributes, including NOL carryforwards and tax credits. The Company performed an estimated analysis to determine if any additional ownership changes occurred during the year ended December 31 ,2025 and changes were identified. Since the Company identified changes in ownership, the Company's deferred tax assets related to its federal and state NOLs in the amount of $27,147,000 and $7,236,000, respectively, were eliminated or restricted. Therefore, the Company removed the related asset from the deferred tax asset schedule with a corresponding reduction in the valuation allowance.

The Company had federal definite life NOL carryforwards of approximately $1,938,000 and $30,512,000 at December 31, 2025 and 2024, respectively. Approximately $1,938,000 of the NOL carryforwards begin to expire during the year ended December 31, 2025 and become fully expired by 2038 and approximately $141,991,000 of NOL carryforwards, which were generated after the enactment of Tax Cuts and Jobs Act, have an indefinite life.

The valuation allowance was $34,311,000 and $60,096,000 at December 31, 2025 and 2024, respectively. The decrease of approximately $25,785,000 between 2025 and 2024 is primarily due to adjustments to the domestic deferred tax assets related to NOLs.

The Company's gross deferred tax asset for state net operating losses and the valuation allowance for the year ended December 31, 2025 are each reduced by $152,000 to apply the IRC Section 382 NOL utilization limitation to the state of California NOLs.

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The reconciliation of the statutory federal income tax rate to the Company's effective tax rate for the years ended December 31, 2025 and 2024 was as follows:

---

| | | |
|:---|:---|:---|
|  | **Year Ended December 31,**  | **Year Ended December 31,**  |
|  | **2025** | **2024** |
| Tax at federal statutory rate | 21% | 21% |
| State tax, net of federal benefit | 1 | 3 |
| Change in valuation allowance | 201 | (22) |
| Change in warrant valuation | (9) | 2 |
| Net operating loss reduction due to Section 382 limitation | (213) | (1) |
| Permanent items | (2) | (1) |
| Deferred adjustments, return to provision | 1 | (2) |
| Effective income tax rate | -% | -% |

---

The Company files income tax returns in the U.S. and in various state jurisdictions with varying statutes of limitations. The Company has not been audited by the Internal Revenue Service or any state income or franchise tax agency. As of December 31, 2025, the Company's federal returns for the years ended 2023 through the current period and most state returns for the years ended 2022 through the current period are still open to examination. In addition, all of the net operating losses and research and development credits generated in years earlier than 2022 and 2021, respectively, are still subject to Internal Revenue Service audit. The federal and California tax returns for the year ended December 31, 2024 reflect research and development carryforwards of $3,401,000 and $5,856,000, respectively. The Company does not anticipate claiming any additional research and development credits for the year ended December 31, 2025.

As of December 31, 2025, the Company's gross unrecognized tax benefits are approximately $0. A reconciliation of the change in the Company's unrecognized tax benefits is as follows:

---

| | | | |
|:---|:---|:---|:---|
| **(in thousands)** | **Federal Tax** | **State Tax** | **Total** |
| Balance at December 31, 2023 | $4657 | $6067 | $10724 |
| &nbsp;&nbsp;Return to provision true up | (282) | (211) | (493) |
| &nbsp;&nbsp;Decrease due to expirations during 2024 | (974) | - | (974) |
| Balance at December 31, 2024 | 3401 | 5856 | 9257 |
| &nbsp;&nbsp;Decrease due to Section 383 limitations during 2025 | (3401) | (5856) | (9257) |
| Balance at December 31, 2025 | $- | $- | $- |

---

The decrease for the year ended December 31, 2025 is related to the section 383 limitation carryforward.

The Company does not expect its unrecognized tax benefits to change significantly over the next 12 months. The Company recognizes interest and penalties related to unrecognized tax benefits within the interest expense line and other expense line, respectively, in its consolidated statement of operations and comprehensive loss. The Company has not recorded any interest or penalties as a result of uncertain tax positions as of December 31, 2025 and 2024. Accrued interest and penalties would be included within the related liability in the consolidated balance sheet.

The Company adopted ASU 2023-09, Improvements to Income Tax Disclosures (ASC 740), effective for the year ended December 31, 2025. ASU 2023-09 requires enhanced annual disclosures of the income tax rate reconciliation and income taxes paid, including disaggregation of reconciling items and cash taxes paid by jurisdiction, with separate disclosure for any reconciling item that equals or exceeds 5 percent of the amount computed by multiplying income (loss) from continuing operations before income taxes by the applicable statutory federal income tax rate. In connection with the adoption, the Company evaluated the disaggregation of its rate reconciliation and income taxes paid and determined that, other than the categories presented in the tables above, no additional reconciling items met the 5 percent significance threshold or were otherwise material for separate disclosure. For the year ended December 31, 2025, income taxes paid to the State of Texas totaled $10,712. Except for this additional disclosure, the adoption of ASU 2023-09 did not have a material impact on the Company's income tax disclosures.

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#### NOTE 12: RELATED PARTY TRANSACTIONS
On December 1, 2023, the Company entered into a consulting agreement with Biodesix, Inc. (the "Biodesix Agreement") to assist with our miRNA product pipeline. Jack Schuler, a beneficial owner of more than 10% of the Company's stock, is also a beneficial owner of more than 10% of the stock of Biodesix, Inc. Since the inception of the Biodesix Agreement, the Company has recorded $125,000 in costs under the Biodesix Agreement as research and development expense in its consolidated financial statement of operations. As of December 31, 2025 and 2024, the Company had $0 and $53,000, respectively, recorded as a current liability for Biodesix, Inc. in its consolidated balance sheet.

#### NOTE 13: SUBSEQUENT EVENTS
Subsequent to December 31, 2025 and through the date of this filing, the Company sold 20,000 shares under the 2025 Lincoln Park Agreement for gross proceeds of approximately $6,000. As of the date of issuance, total gross proceeds to the Company over the life of the 2025 Lincoln Park Agreement is $6,000 and the value of the remaining availability was approximately $9,994,000 that could be sold to Lincoln Park under the 2025 Lincoln Park Agreement, subject to the terms of the 2025 Lincoln Park Agreement.

On January 30, 2026, the Company entered into a Subordinated Business Loan and Security Agreement (the "Subordinated Loan Agreement") with Agile Lending, LLC, as lead lender, and Agile Capital Funding, LLC, as collateral agent, pursuant to which the Lenders (as such term is defined in the Subordinated Loan Agreement) agreed to make a secured term loan to the Company and certain subsidiary co-borrowers.

The term loan is evidenced by a Subordinated Secured Promissory Note (the "Promissory Note"), which was issued in the principal amount of $1,050,000, will include interest charges of $441,000, and is scheduled to mature on August 26, 2026. The Promissory Note is expressly subordinated in right of payment to all Senior Indebtedness, as described in the Promissory Note. The collateral agent is authorized to take actions to perfect the security interests; however, the Subordinated Loan Agreement provides that a financing statement may be filed only upon an event of default. The Company will use the proceeds for general corporate purposes.

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#### SIGNATURES
Pursuant to the requirements of Section 13 or 15(d) of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized.

---

| | |
|:---|:---|
|  | **Aspira Women's Health Inc.** |
| Date: April 1, 2026 | */s/ Michael Buhle* |
|  | Michael Buhle |
|  | President and Chief Executive Officer (Principal Executive Officer) |
| Date: April 1, 2026 | */s/ Brian Hungerford* |
|  | Brian Hungerford |
|  | Chief Financial Officer (Principal Financial Officer and Principal Accounting Officer) |

---

[**Table of Contents**](#TOC)

#### POWER OF ATTORNEY
Each of the undersigned officers and directors of Aspira Women's Health Inc., hereby constitutes and appoints Michael Buhle and Brian Crawford, and each or any of them, as their true and lawful attorney-in-fact and agent, for them and in their name, place and stead, in any and all capacities, to sign their name to any and all amendments to this Report on Form 10-K, and other related documents, and to cause the same to be filed with the Securities and Exchange Commission, granting unto said attorneys, full power and authority to do and perform any act and thing necessary and proper to be done in the premises, as fully to all intents and purposes as the undersigned could do if personally present, and the undersigned for himself hereby ratifies and confirms all that said attorney shall lawfully do or cause to be done by virtue hereof.

Pursuant to the requirements of the Securities Exchange Act of 1934, this report has been signed below by the following persons on behalf of the registrant and in the capacities and on the dates indicated.

---

| | | |
|:---|:---|:---|
| **Name** | **Title** | **Date** |
| */s/ Michael Buhle* | President and Chief Executive Officer |  |
| Michael Buhle | (Principal Executive Officer) and Director | April 1, 2026 |
| */s/ Brian Hungerford* | Chief Financial Officer |  |
| Brian Hungerford | (Principal Financial Officer and Principal Accounting Officer) | April 1, 2026 |
| */s/ John Fraser* | Chair of the Board of Directors | April 1, 2026 |
| John Fraser |  |  |
| */s/ Ellen Beausang* | Director | April 1, 2026 |
| Ellen Beausang |  |  |
| */s/ Jeffrey Cohen* | Director | April 1, 2026 |
| Jeffrey Cohen |  |  |
| */s/ Cynthia Hundorfean* | Director | April 1, 2026 |
| Cynthia Hundorfean |  |  |
| */s/ Winfred Parnell* | Director | April 1, 2026 |
| Winfred Parnell |  |  |
| */s/ Jane Pine Wood* | Director | April 1, 2026 |
| Jane Pine Wood |  |  |

---

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## Exhibit 23.1

**EXHIBIT 23.1**

<u>Consent of Independent Registered Public Accounting Firm</u>

We hereby consent to the incorporation by reference in the Registration Statements on Form S-3 (No. 333-189929, 333-217249, 333-248920, 333-281745, 333-280848 and 333-278867) and Form S-8 (No. 333-167204, 333-193312, 333-205855, 333-226462, 333-232541, 333-276728, 333-281665 and 333-287878) of Aspira Women's Health, Inc. (the Company) of our report dated April 1, 2026, relating to the consolidated financial statements which appears in this Annual Report on Form 10-K. Our report contains an explanatory paragraph regarding the Company's ability to continue as a going concern.

 

/s/ BDO USA, P.C.

Boston, Massachusetts

April 1, 2026

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## Exhibit 31.1

**Exhibit 31.1**

**CERTIFICATION**

I, Michael Buhle, certify that:

&nbsp;&nbsp;&nbsp;&nbsp;1. I have reviewed this annual report on Form 10-K for the year ended December 31, 2025 of Aspira Women's Health Inc.;

&nbsp;&nbsp;&nbsp;&nbsp;2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report;

&nbsp;&nbsp;&nbsp;&nbsp;3. Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material respects the financial condition, results of operations and cash flows of the registrant as of, and for, the periods presented in this report;

&nbsp;&nbsp;&nbsp;&nbsp;4. The registrant's other certifying officer(s) and I are responsible for establishing and maintaining disclosure controls and procedures (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act Rules 13a-15(f) and 15d-15(f)) for the registrant and have:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) Designed such disclosure controls and procedures, or caused such disclosure controls and procedures to be designed under our supervision, to ensure that material information relating to the registrant, including its consolidated subsidiaries, is made known to us by others within those entities, particularly during the period in which this report is being prepared;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) Designed such internal control over financial reporting, or caused such internal control over financial reporting to be designed under our supervision, to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for external purposes in accordance with generally accepted accounting principles;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(c) Evaluated the effectiveness of the registrant's disclosure controls and procedures and presented in this report our conclusions about the effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(d) Disclosed in this report any change in the registrant's internal control over financial reporting that occurred during the registrant's most recent fiscal quarter (the registrant's fourth fiscal quarter in the case of an annual report) that has materially affected, or is reasonably likely to materially affect, the registrant's internal control over financial reporting; and

&nbsp;&nbsp;&nbsp;&nbsp;5. The registrant's other certifying officer(s) and I have disclosed, based on our most recent evaluation of internal control over financial reporting, to the registrant's auditors and the audit committee of the registrant's board of directors (or persons performing the equivalent functions):

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) All significant deficiencies and material weaknesses in the design or operation of internal control over financial reporting which are reasonably likely to adversely affect the registrant's ability to record, process, summarize and report financial information; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) Any fraud, whether or not material, that involves management or other employees who have a significant role in the registrant's internal control over financial reporting.

---

| | |
|:---|:---|
| Date: April 1, 2026 | */s/ Michael Buhle* |
|  | Michael Buhle  |
|  | Chief Executive Officer  |
|  | (Principal Executive Officer) |

---

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## Exhibit 31.2

**EXHIBIT 31.2**

**CERTIFICATION**

I, Brian Hungerford, certify that:

&nbsp;&nbsp;&nbsp;&nbsp;1. I have reviewed this annual report on Form 10-K for the year ended December 31, 2025 of Aspira Women's Health Inc.;

&nbsp;&nbsp;&nbsp;&nbsp;2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report;

&nbsp;&nbsp;&nbsp;&nbsp;3. Based on my knowledge, the financial statements, and other financial information included in this report, fairly present in all material respects the financial condition, results of operations and cash flows of the registrant as of, and for, the periods presented in this report;

&nbsp;&nbsp;&nbsp;&nbsp;4. The registrant's other certifying officer(s) and I are responsible for establishing and maintaining disclosure controls and procedures (as defined in Exchange Act Rules 13a-15(e) and 15d-15(e)) and internal control over financial reporting (as defined in Exchange Act Rules 13a-15(f) and 15d-15(f)) for the registrant and have:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) Designed such disclosure controls and procedures, or caused such disclosure controls and procedures to be designed under our supervision, to ensure that material information relating to the registrant, including its consolidated subsidiaries, is made known to us by others within those entities, particularly during the period in which this report is being prepared;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) Designed such internal control over financial reporting, or caused such internal control over financial reporting to be designed under our supervision, to provide reasonable assurance regarding the reliability of financial reporting and the preparation of financial statements for external purposes in accordance with generally accepted accounting principles;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(c) Evaluated the effectiveness of the registrant's disclosure controls and procedures and presented in this report our conclusions about the effectiveness of the disclosure controls and procedures, as of the end of the period covered by this report based on such evaluation; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(d) Disclosed in this report any change in the registrant's internal control over financial reporting that occurred during the registrant's most recent fiscal quarter (the registrant's fourth fiscal quarter in the case of an annual report) that has materially affected, or is reasonably likely to materially affect, the registrant's internal control over financial reporting; and

&nbsp;&nbsp;&nbsp;&nbsp;5. The registrant's other certifying officer(s) and I have disclosed, based on our most recent evaluation of internal control over financial reporting, to the registrant's auditors and the audit committee of the registrant's board of directors (or persons performing the equivalent functions):

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) All significant deficiencies and material weaknesses in the design or operation of internal control over financial reporting which are reasonably likely to adversely affect the registrant's ability to record, process, summarize and report financial information; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) Any fraud, whether or not material, that involves management or other employees who have a significant role in the registrant's internal control over financial reporting.

---

| | |
|:---|:---|
| Rian h<br>|  |
| Date: April 1, 2026 | */s/ Brian Hungerford* |
|  | Brian Hungerford |
|  | Chief Financial Officer |
|  | (Principal Financial Officer and Principal Accounting Officer) |

---

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## Exhibit 32.1

**Exhibit 32.1**

**Certification**

**Pursuant to 18 U.S.C. Section 1350, as Adopted Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002**

**with Respect to the Annual Report on Form 10-K**

**for the Year Ended December 31, 2025**

Pursuant to the requirement set forth in Rule 13a-14(b) of the Securities Exchange Act of 1934, as amended, (the "Exchange Act") and Section 1350 of Chapter 63 of Title 18 of the United States Code (18 U.S.C. § 1350), Michael Buhle, Chief Executive Officer of Aspira Women's Health Inc., a Delaware corporation (the "Company"), and James Crawford, Vice President of Finance of the Company, each hereby certifies, to the best of her and his knowledge, that:

&nbsp;&nbsp;&nbsp;&nbsp;1. The Company's annual report on Form 10-K for the year ended December 31, 2025, to which this Certification is attached as Exhibit 32.1 (the "Form 10-K"), fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934, as amended; and

&nbsp;&nbsp;&nbsp;&nbsp;2. The information contained in the Form 10-K fairly presents, in all material respects, the financial condition and results of operations of the Company.

---

| | |
|:---|:---|
| Date: April 1, 2026 | */s/ Michael Buhle* |
|  | Michael Buhle |
|  | Chief Executive Officer |
|  | (Principal Executive Officer) |

---

---

| | |
|:---|:---|
| Date: April 1, 2026 | */s/ Brian Hungerford* |
|  | Brian Hungerford |
|  | Chief Financial Officer |
|  | (Principal Financial Officer and Principal Accounting Officer) |

---

The certification set forth above is being furnished as an Exhibit solely pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 and is not being filed as part of the Form 10-K/A or as a separate disclosure document of the Company or the certifying officers.

------