# EDGAR Filing Document

**Accession Number:** 0001807166
**File Stem:** 0001213900-23-019694
**Filing Date:** 2023-3
**Character Count:** 117785
**Document Hash:** 7e1c77ec99beed2539dc7e0f445bdcee
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001213900-23-019694.hdr.sgml**: 20230314

**ACCESSION NUMBER**: 0001213900-23-019694

**CONFORMED SUBMISSION TYPE**: S-1

**PUBLIC DOCUMENT COUNT**: 5

**FILED AS OF DATE**: 20230314

**DATE AS OF CHANGE**: 20230313

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** Amesite Inc.
- **CENTRAL INDEX KEY:** 0001807166
- **STANDARD INDUSTRIAL CLASSIFICATION:** SERVICES-PREPACKAGED SOFTWARE [7372]
- **IRS NUMBER:** 823431717
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 0630

**FILING VALUES:**
- **FORM TYPE:** S-1
- **SEC ACT:** 1933 Act
- **SEC FILE NUMBER:** 333-270512
- **FILM NUMBER:** 23729022

**BUSINESS ADDRESS:**
- **STREET 1:** 205 EAST WASHINGTON STREET
- **STREET 2:** SUITE B
- **CITY:** ANN ARBOR
- **STATE:** MI
- **ZIP:** 48104
- **BUSINESS PHONE:** (650) 516-7633

**MAIL ADDRESS:**
- **STREET 1:** 205 EAST WASHINGTON STREET
- **STREET 2:** SUITE B
- **CITY:** ANN ARBOR
- **STATE:** MI
- **ZIP:** 48104

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** Amesite Operating Co
- **DATE OF NAME CHANGE:** 20200318

**As filed with the Securities and Exchange Commission on March 14, 2023**

**Registration No. 333-**

**UNITED STATES**

**SECURITIES AND EXCHANGE COMMISSION**

**WASHINGTON, D.C. 20549**

**FORM S-1**

**REGISTRATION STATEMENT**

***UNDER***

***THE SECURITIES ACT OF 1933***

**AMESITE INC.**

(Exact name of registrant as specified in its charter)

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| | | |
|:---|:---|:---|
| **Delaware** | **7372** | **82-3431718** |
| (State or other jurisdiction of<br> incorporation or organization) | (Primary Standard Industrial<br> Classification Code Number) | (I.R.S. Employer<br> Identification Number) |

---

**607 Shelby Street**

**Suite 700 PMB 214**

**Detroit, MI**

**(734) 876-8130** 

(Address, including zip code, and telephone number, including area code, of registrant's principal executive offices)

**Ann Marie Sastry**

**Chief Executive Officer**

**Amesite Inc.**

**607 Shelby Street, Suite 700 PMB 214**

**Detroit, MI 48226**

**(734) 876-8130**

(Name, address, including zip code, and telephone number, including area code, of agent for service)

***Copies to:***

**Richard A. Friedman, Esq.**

**Sean F. Reid, Esq.**

**Sheppard, Mullin, Richter & Hampton LLP**

**30 Rockefeller Plaza**

**New York, NY 10112**

**Tel: (212) 653-8700**

**Fax: (212) 653-8701**

**Approximate date of commencement of proposed sale to the public**: As soon as practicable after the effective date of this registration statement.

If any of the securities being registered on this Form are to be offered on a delayed or continuous basis pursuant to Rule 415 under the Securities Act of 1933 check the following box. ☒

If this Form is filed to register additional securities for an offering pursuant to Rule 462(b) under the Securities Act, please check the following box and list the Securities Act registration statement number of the earlier effective registration statement for the same offering. ☐

If this Form is a post-effective amendment filed pursuant to Rule 462(c) under the Securities Act, check the following box and list the Securities Act registration statement number of the earlier effective registration statement for the same offering. ☐

If this Form is a post-effective amendment filed pursuant to Rule 462(d) under the Securities Act, check the following box and list the Securities Act registration number of the earlier effective registration statement for the same offering. ☐

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company or an emerging growth company. See the definitions of "large accelerated filer," "accelerated filer," "smaller reporting company" and "emerging growth company" in Rule 12b-2 of the Exchange Act.

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| | | | |
|:---|:---|:---|:---|
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Large accelerated filer | ☐ | Accelerated filer | ☐ |
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Non-accelerated filer | ☒ | Smaller reporting company | ☒ |
|  |  | Emerging growth company | ☒ |

---

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 7(a)(2)(B) of Securities Act. ☐

**THE REGISTRANT HEREBY AMENDS THIS REGISTRATION STATEMENT ON SUCH DATE OR DATES AS MAY BE NECESSARY TO DELAY ITS EFFECTIVE DATE UNTIL THE REGISTRANT SHALL FILE A FURTHER AMENDMENT WHICH SPECIFICALLY STATES THAT THIS REGISTRATION STATEMENT SHALL THEREAFTER BECOME EFFECTIVE IN ACCORDANCE WITH SECTION 8(a) OF THE SECURITIES ACT OF 1933, AS AMENDED, OR UNTIL THE REGISTRATION STATEMENT SHALL BECOME EFFECTIVE ON SUCH DATE AS THE SECURITIES AND EXCHANGE COMMISSION, ACTING PURSUANT TO SAID SECTION 8(a), MAY DETERMINE.**

**THE INFORMATION IN THIS PROSPECTUS IS NOT COMPLETE AND MAY BE CHANGED. THE SELLING STOCKHOLDERS MAY NOT RESELL THESE SECURITIES UNTIL THE REGISTRATION STATEMENT FILED WITH THE SECURITIES AND EXCHANGE COMMISSION IS DECLARED EFFECTIVE. THIS PROSPECTUS IS NOT AN OFFER TO SELL THESE SECURITIES AND IS NOT SOLICITING AN OFFER TO BUY THESE SECURITIES IN ANY STATE WHERE THE OFFER OR SALE IS NOT PERMITTED.**

SUBJECT TO COMPLETION, DATED MARCH 14, 2023

**PROSPECTUS**

**Amesite Inc.**

**366,665 Shares of Common Stock**

The selling stockholders of Amesite Inc. ("Amesite," "we," "us" or the "Company") listed beginning on page 12 of this prospectus may offer and resell under this prospectus up to (i) 349,240 shares of our common stock, par value $0.0001 per share (the "common stock"), issuable upon exercise of warrants (the "warrants") acquired by certain of the selling stockholders under the Purchase Agreements (defined below), (ii) up to 17,425 shares of our common stock issuable upon exercise of placement agent warrants (the "placement agent warrants" and, together with the warrants, the "warrants") acquired by certain of the selling stockholders under the Placement Agent Agreement (defined below). The selling stockholders acquired the warrants from us pursuant to securities purchase agreements (the "Purchase Agreements"), dated August 30, 2022, by and between the Company and each of the purchasers named therein and placement agency agreement, dated August 30, 2022, by and among the Company and Laidlaw & Company (UK) Ltd. (the "Placement Agent Agreement").

We are registering the resale of the shares of common stock covered by this prospectus as required by the Purchase Agreements. The selling stockholders will receive all of the proceeds from any sales of the shares of common stock offered hereby. We will not receive any of the proceeds, but we will incur expenses in connection with the offering. To the extent the warrants are exercised for cash, if at all, we will receive the exercise price of the warrants.

The selling stockholders may sell these shares through public or private transactions at market prices prevailing at the time of sale or at negotiated prices. The timing and amount of any sale are within the sole discretion of the selling stockholders. Our registration of the shares of common Stock covered by this prospectus does not mean that the selling stockholders will offer or sell any of the shares. For further information regarding the possible methods by which the shares may be distributed, see "Plan of Distribution" beginning on page 13 of this prospectus.

Our common stock is listed on The Nasdaq Capital Market under the symbol "AMST." The last reported sale price of our common stock on March 10, 2023 was $2.80 per share.

We are an "emerging growth company" under applicable Securities and Exchange Commission rules and, as such, we are subject to reduced public company reporting requirements.

**Investing in our common stock is highly speculative and involves a significant degree of risk. Please consider carefully the specific factors set forth under "Risk Factors" beginning on page 6 of this prospectus and in our filings with the Securities and Exchange Commission.**

**Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or determined if this prospectus is truthful or complete. Any representation to the contrary is a criminal offense.**

The date of this prospectus is , 2023.

**TABLE OF CONTENTS**

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| | |
|:---|:---|
| [PROSPECTUS SUMMARY](#a_001) | 1 |
| [THE OFFERING](#a_002) | 5 |
| [RISK FACTORS](#a_003) | 6 |
| [THE PRIVATE PLACEMENT](#a_004) | 7 |
| [SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS](#a_005) | 8 |
| [USE OF PROCEEDS](#a_006) | 9 |
| [MARKET FOR COMMON STOCK AND DIVIDEND POLICY](#a_007) | 10 |
| [SELLING STOCKHOLDERS](#a_008) | 12 |
| [PLAN OF DISTRIBUTION](#a_009) | 13 |
| [DESCRIPTION OF OUR SECURITIES TO BE REGISTERED](#a_010) | 15 |
| [LEGAL MATTERS](#a_011) | 19 |
| [EXPERTS](#a_012) | 19 |
| [WHERE YOU CAN FIND MORE INFORMATION](#a_013) | 19 |
| [INCORPORATION OF DOCUMENTS BY REFERENCE](#a_014) | 20 |

---

i

**ABOUT THIS PROSPECTUS**

The registration statement we filed with the Securities and Exchange Commission (the "SEC") includes exhibits that provide more detail of the matters discussed in this prospectus. You should read this prospectus, the related exhibits filed with the SEC, and the documents incorporated by reference herein before making your investment decision. You should rely only on the information provided in this prospectus and the documents incorporated by reference herein or any amendment thereto. In addition, this prospectus contains summaries of certain provisions contained in some of the documents described herein, but reference is made to the actual documents for complete information. All of the summaries are qualified in their entirety by the actual documents. Copies of some of the documents referred to herein have been filed, will be filed or will be incorporated by reference as exhibits to the registration statement of which this prospectus is a part, and you may obtain copies of those documents as described below under the heading "Where You Can Find More Information."

The selling stockholders named in this prospectus may sell up to 366,665 shares of our common stock previously issued and issuable upon exercise of warrants to purchase shares of our common stock from time to time. This prospectus also covers any shares of common stock that may become issuable as a result of share splits, share dividends, or similar transactions. We have agreed to pay the expenses incurred in registering these shares, including legal and accounting fees.

We have not, and the selling stockholders have not, authorized anyone to provide any information or to make any representations other than those contained in this prospectus, the documents incorporated by reference herein or in any free writing prospectuses prepared by or on behalf of us or to which we have referred you. We take no responsibility for, and can provide no assurance as to the reliability of, any other information that others may give you. The information contained in this prospectus, the documents incorporated by reference herein or in any applicable free writing prospectus is current only as of its date, regardless of its time of delivery or any sale of our securities. Our business, financial condition, results of operations and prospects may have changed since that date.

The selling stockholders are offering to sell, and seeking offers to buy, shares of our common stock only under circumstances and in jurisdictions where it is lawful to do so. The selling stockholders are not making an offer to sell these securities in any state or jurisdiction where the offer or sale is not permitted.

Unless the context otherwise requires, "Amesite," "AMST," "the Company," "we," "us," "our" and similar terms refer to Amesite Inc.

**Industry and Market Data**

This prospectus or the documents incorporated by reference herein includes statistical and other industry and market data that we obtained from industry publications and research, surveys and studies conducted by third parties. Industry publications and third-party research, surveys and studies generally indicate that their information has been obtained from sources believed to be reliable, although they do not guarantee the accuracy or completeness of such information.

ii

**PROSPECTUS SUMMARY**

*The following is a summary of what we believe to be the most important aspects of our business and the offering of our securities under this prospectus. We urge you to read this entire prospectus, including the more detailed consolidated financial statements, notes to the consolidated financial statements and other information incorporated by reference from our other filings with the SEC or included in any applicable prospectus supplement. Investing in our securities involves risks. Therefore, carefully consider the risk factors set forth in any prospectus supplements and in our most recent annual and quarterly filings with the SEC, as well as other information in this prospectus and any prospectus supplements and the documents incorporated by reference herein or therein, before purchasing our securities. Each of the risk factors could adversely affect our business, operating results and financial condition, as well as adversely affect the value of an investment in our securities.*

**Overview**

Amesite's smart, intuitive learning environments help organizations thrive. Amesite is a high tech artificial intelligence software company offering a cloud-based platform and content creation services for business and university-delivered education and upskilling. Amesite-offered courses and programs are branded to our part. Amesite uses artificial intelligence technologies to provide customized environments for learners, easy-to-manage interfaces for instructors, and greater accessibility for learners in the US education market and beyond. We leverage existing institutional infrastructures, adding mass customization and cutting-edge technology to provide cost-effective, scalable and engaging experiences for learners anywhere.

We are passionate about improving the learner experience and learner outcomes in online learning products, and improving our Customers' ability to create and deliver both. We are focused on creating the best possible technology solutions and have been awarded an innovation award for our product. We are committed to our team, and have been recognized with 10 workplace excellence awards, 4 of them national.

Amesite offers our white label platform to our customers: universities, museums, businesses and government agencies. Our customers offer learning to their users, who are students, professional learners and / or their own employees. Amesite derives revenue from the licensing of our platform, and user fees associated with its use by our customers for their users. Some of our customers generate revenue using our systems, including universities and museums.

**Recent Developments**

*Reverse Stock Split*

On February 15, 2023, the Company held a special meeting of stockholders (the "Special Meeting").

At the Special Meeting, the stockholders approved a proposal to amend the Company's certificate of incorporation to effect a reverse split of the Company's outstanding shares of common stock, par value $0.0001 at a specific ratio within a range of one-for five (1-for-5) to a maximum of one-for-fifty (1-for-50) to be determined by the Company's board of directors in its sole discretion.

Following the Special Meeting, the board of directors approved a one-for-twelve (1-for-12) reverse split of the Company's issued and outstanding shares of common stock (the "Reverse Stock Split"). On February 21, 2023, the Company filed with the Secretary of State of the State of Delaware a certificate of amendment to its certificate of incorporation (the "Certificate of Amendment") to effect the Reverse Stock Split. The Reverse Stock Split became effective as of 4:01 p.m. Eastern Time on February 21, 2023, and the Company's common stock is expected to begin trading on a split-adjusted basis when the Nasdaq Stock Market opens on February 22, 2023.

When the Reverse Stock Split became effective, every twelve (12) shares of the Company's issued and outstanding common stock were automatically combined, converted and changed into one (1) share the Company's common stock, without any change in the number of authorized shares or the par value per share. In addition, a proportionate adjustment was made to the per share exercise price and the number of shares issuable upon the exercise of all outstanding stock options, restricted stock units and warrants to purchase shares of common stock and the number of shares reserved for issuance pursuant to the company's equity incentive compensation plans. Any fraction of a share of common stock that would be created as a result of the Reverse Stock Split was rounded up to the next whole share.

All share and per share information in this prospectus (other than the historical financial statements incorporated by reference herein) has been adjusted to reflect the reverse stock split.

**Implication of Being an Emerging Growth Company**

We are an "emerging growth company" as defined in the Jumpstart Our Business Startups Act of 2012, as amended (the "JOBS Act"). We will remain an emerging growth company until the earlier of (1) the last day of the fiscal year following the fifth anniversary of the completion of our initial public offering, (2) the last day of the fiscal year in which we have total annual gross revenues of at least $1.07 billion, (3) the date on which we are deemed to be a "large accelerated filer" as defined in Rule 12b-2 under the Securities Exchange Act of 1934, as amended (the "Exchange Act"), which would occur if the market value of our common stock held by non-affiliates exceeded $700.0 million as of the last business day of our most recently completed second fiscal quarter or (4) the date on which we have issued more than $1.0 billion in non-convertible debt securities during the prior three-year period. An emerging growth company may take advantage of specified reduced reporting requirements and is relieved of certain other significant requirements that are otherwise generally applicable to public companies. As an emerging growth company,

● we may reduce our executive compensation disclosure;

● we may present only two years of audited financial statements, plus unaudited condensed financial statements for any interim period, and related Management's Discussion and Analysis of Financial Condition and Results of Operations in this Prospectus;

● we may avail ourselves of the exemption from the requirement to obtain an attestation and report from our auditors on the assessment of our internal control over financial reporting pursuant to the Sarbanes-Oxley Act of 2002; and

● we may not require stockholder non-binding advisory votes on executive compensation or golden parachute arrangements.

We have availed ourselves in this Prospectus of the reduced reporting requirements described above with respect to compensation disclosure requirements and selected financial data. As a result, the information that we provide stockholders may be less comprehensive than what you might receive from other public companies. When we are no longer deemed to be an emerging growth company, we will not be entitled to the exemptions provided in the JOBS Act discussed above. We have not elected to avail ourselves of the exemption that allows emerging growth companies to extend the transition period for complying with new or revised financial accounting standards. This election is irrevocable.

As a company with less than $1.0 billion in revenue during our last fiscal year, we qualify as an "emerging growth company."

**Smaller Reporting Company**

We are also currently a "smaller reporting company," meaning that we are not an investment company, an asset-backed issuer, or a majority-owned subsidiary of a parent company that is not a smaller reporting company, and have a public float of less than $250 million or annual revenues of less than $100 million during the most recently completed fiscal year. In the event that we are still considered a "smaller reporting company," at such time as we cease being an "emerging growth company," the disclosure we will be required to provide in our SEC filings will increase, but will still be less than it would be if we were not considered either an "emerging growth company" or a "smaller reporting company." Specifically, similar to "emerging growth companies," "smaller reporting companies" are able to provide simplified executive compensation disclosures in their filings; are exempt from the provisions of Section 404(b) of the Sarbanes-Oxley Act requiring that independent registered public accounting firms provide an attestation report on the effectiveness of internal control over financial reporting; and have certain other decreased disclosure obligations in their SEC filings, including, among other things, only being required to provide two years of audited financial statements in annual reports. Decreased disclosures in our SEC filings due to our status as an "emerging growth company" or "smaller reporting company" may make it harder for investors to analyze our results of operations and financial prospects.

**Risks Associated with Our Business**

Our business and our ability to implement our business strategy are subject to numerous risks, as more fully described in the section entitled "Risk Factors" in this prospectus and in our Annual Report on Form 10-K for the fiscal year ended June 30, 2022, incorporated herein by reference. You should read these risks before you invest in our securities. We may be unable, for many reasons, including those that are beyond our control, to implement our business strategy.

**Corporate Information and History**

The Company was incorporated in November 2017. The Company is an artificial intelligence driven platform and course designer, that provides customized, high performance and scalable online products for schools and businesses. The Company uses machine learning to provide a novel, mass customized experience to learners. The Company's customers are businesses, universities and colleges, and K-12 schools. The Company's activities are subject to significant risks and uncertainties. The Company's operations are in one segment.

On September 18, 2020, we consummated a reorganizational merger (the "Reorganization"), pursuant to an Agreement and Plan of Merger (the "Merger Agreement"), dated July 14, 2020, whereby Amesite Inc. ("Amesite Parent"), our former parent corporation, merged with and into us, with our Company resulting as the surviving entity. In connection with the same, we filed a Certificate of Ownership and Merger with the Secretary of State of the State of Delaware, and changed our name from "Amesite Operating Company" to "Amesite Inc." The stockholders of Amesite Parent approved the Merger Agreement on August 4, 2020. The directors and officers of Amesite Parent became our directors and officers.

Pursuant to the Merger Agreement, on the Effective Date, each share of Amesite Parent's common stock, $0.0001 par value per share, issued and outstanding immediately before the Effective Date, was converted, on a one-for-one basis, into shares of our common stock. Additionally, each option or warrant to acquire shares of Amesite Parent outstanding immediately before the Effective Date was converted into and became an equivalent option to acquire shares of our common stock, upon the same terms and conditions.

Our corporate headquarters are located at 607 Shelby Street, Suite 700 PMB 214, Detroit, Michigan 48226, and our telephone number is (734) 876-8130. We maintain a website at www.amesite.com. The contents of, or information accessible through, our website are not part of this Annual Report on Form 10-K, and our website address is included in this document as an inactive textual reference only. We make our filings with the SEC, including our Annual Reports on Form 10-K, Quarterly Reports on Form 10-Q, Current Reports on Form 8-K and all amendments to those reports, available free of charge on our website as soon as reasonably practicable after we file such reports with, or furnish such reports to, the SEC. The public may read and copy the materials we file with the SEC at the SEC's Public Reference Room at 100 F Street, NE, Washington, D.C. 20549. The public may also obtain information on the operation of the Public Reference Room by calling the SEC at 1-800-SEC-0330. Additionally, the SEC maintains an internet site that contains reports, proxy and information statements and other information. The address of the SEC's website is www.sec.gov. The information contained in the SEC's website is not intended to be a part of this filing.

**THE OFFERING**

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| | |
|:---|:---|
| **Shares of Common Stock that May be Offered by the Selling Stockholders** | Up to 366,665 shares of common stock. |
| **Use of Proceeds** | We will not receive any proceeds from the sale of the common stock by the selling stockholders. However, if all of the warrants were exercised for cash, we would receive gross proceeds of approximately $3.6 million. See the section entitled "Use of Proceeds" in this prospectus. |
| **Offering Price** | The selling stockholders may sell all or a portion of their shares through public or private transactions at prevailing market prices or at privately negotiated prices. |
| **Nasdaq Capital Market Symbol** | AMST |
| **Risk Factors** | Investing in our common stock involves a high degree of risk. See "Risk Factors" beginning on page 6 of this prospectus, and any other risk factors described in the documents incorporated by reference herein, for a discussion of certain factors to consider carefully before deciding to invest in our common stock. |

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Throughout this prospectus, when we refer to the shares of our common stock being registered on behalf of the selling stockholders for offer and sale, we are referring to the shares of common stock issuable upon exercise of the warrants, each as described under "The Private Placement" and "Selling Stockholders." When we refer to the selling stockholders in this prospectus, we are referring to the selling stockholders identified in this prospectus and, as applicable, their donees, pledgees, transferees or other successors-in-interest selling shares of common stock or interests in shares of common stock received after the date of this prospectus from a selling stockholder as a gift, pledge, partnership distribution or other transfer.

**RISK FACTORS**

Investing in our securities involves a high degree of risk. You should carefully consider and evaluate all of the information contained in this prospectus and in the documents we incorporate by reference into this prospectus before you decide to purchase our securities. In particular, you should carefully consider and evaluate the risks and uncertainties described under the heading "Risk Factors" in our Annual Report on Form 10-K for the year ended June 30, 2022. Any of the risks and uncertainties set forth below and in the Annual Report, as updated by annual, quarterly and other reports and documents that we file with the SEC and incorporate by reference into this prospectus, or any prospectus, could materially and adversely affect our business, results of operations and financial condition, which in turn could materially and adversely affect the value of any securities offered by this prospectus. As a result, you could lose all or part of your investment.

**THE PRIVATE PLACEMENT**

On August 30, 2022 (the "Effective Date"), we entered into a Securities Purchase Agreement (the "Purchase Agreement") with certain investors (the "Investors") for the purpose of raising approximately $2.3 million in gross proceeds for the Company (the "Offering"). Pursuant to the terms of the Purchase Agreement, we agreed to sell, in a registered direct offering, an aggregate of 348,485 shares (the "Shares") of our common stock, par value $0.0001 per share (the "Common Stock"), and in a concurrent private placement, warrants to purchase an aggregate of 348,485 shares (the "Warrant Shares") of Common Stock (the "Warrants" and together with the Shares and the Warrant Shares, the "Securities"), for a combined purchase price per Share and Warrant of $6.60 (the "Purchase Price"). The Warrants will be exercisable commencing six months after the date of their issuance, have an exercise price of $9.84 per share and will expire five and one-half years from the date of issuance.

Laidlaw & Company (UK) Ltd. acted as the exclusive placement agent (the "Placement Agent") for the Company, on a "reasonable best efforts" basis, in connection with the Offering. Pursuant to that certain Placement Agency Agreement, dated as of August 30, 2022, by and between us and the Placement Agent (the "Placement Agency Agreement"), the Placement Agent will be entitled to a cash fee equal to 8.0% of the gross proceeds from the placement of the total amount of Securities sold by the Placement Agent and a cash management fee equal to 1% of the gross proceeds from the placement of the total amount of Securities sold in the Offering. In addition, the Placement Agent will be issued a warrant (the "Placement Agent Warrant") to purchase up to 17,425 shares (the "Placement Agent Warrant Shares") of Common Stock, substantially the same form as the Warrants, at an exercise price of $12.30 per share (125% of the Purchase Price).

The net proceeds to us from the registered direct offering and concurrent private placement, after deducting the Placement Agent's fees and expenses but before paying the Company's estimated offering expenses, and excluding the proceeds, if any, from the exercise of the Warrants and the Placement Agent Warrant, are expected to be approximately $2 million. The Company intends to use the net proceeds from the Offering for working capital and for other general corporate purposes.

Pursuant to the terms of the Purchase Agreement and subject to certain exceptions as set forth in the Purchase Agreement, from the Effective Date until the 75<sup>th</sup> day after the Effective Date, we, may not, without the prior written consent of the Placement Agent and Investors which purchased at least 67.0% in interest of the Shares offered in the Offering, (i) offer, pledge, sell, contract to sell, sell any option or contract to purchase, purchase any option or contract to sell, grant any option, right or warrant to purchase, lend, or otherwise transfer or dispose of, directly or indirectly, any shares of capital stock or any Common Stock Equivalents (as defined in the Purchase Agreement); (ii) except in limited circumstances, file or cause to be filed any registration statement with the Securities and Exchange Commission relating to the offering of any shares of capital stock of the Company or any securities convertible into or exercisable or exchangeable for shares of capital stock of the Company, (iii) complete any offering of debt securities, other than entering into a line of credit with a traditional bank or (iv) enter into any swap or other arrangement that transfers to another, in whole or in part, any of the economic consequences of ownership of capital stock of the Company or any of its subsidiaries, whether any such transaction described in clause (i), (ii), (iii) or (iv) above is to be settled by delivery of shares of capital stock or such other securities, in cash or otherwise.

In connection with the Purchase Agreement and Placement Agency Agreement, our directors, officers, entered into lock-up agreements for a 90-day period (the "Lock-Up Agreements").

The Shares (but not the Warrants or the Warrant Shares) were offered and sold by us pursuant to effective registration statements on Form S-3 (File Nos. 333-260666), as well as a prospectus supplement in connection the Offering filed with the SEC.

The foregoing description of the material terms of the Purchase Agreement, the Warrant, the Placement Agent Warrant, the Placement Agency Agreement and the Lock-Up Agreements does not purport to be complete and is qualified in its entirety by reference to the full text of the form of Purchase Agreement, form of Warrant, form of Placement Agent Warrant, form of Placement Agency Agreement and form of Lock-Up Agreement, copies of which are filed as Exhibits 10.1, 4.1, 4.2, 10.2 and 10.3, respectively, to the Current Report on Form 8-K dated September 1, 2022, and are incorporated herein by reference.

**SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS**

This prospectus and the documents incorporated by reference in this prospectus contain "forward-looking statements," which include information relating to future events, future financial performance, financial projections, strategies, expectations, competitive environment and regulation. Words such as "may," "should," "could," "would," "predicts," "potential," "continue," "expects," "anticipates," "future," "intends," "plans," "believes," "estimates," and similar expressions, as well as statements in future tense, identify forward-looking statements. Forward-looking statements should not be read as a guarantee of future performance or results and may not be accurate indications of when such performance or results will be achieved. Forward-looking statements are based on information we have when those statements are made or management's good faith belief as of that time with respect to future events, and are subject to a number of risks, and uncertainties and assumptions that could cause actual performance or results to differ materially from those expressed in or suggested by the forward-looking statements. These risks are more fully described in the "Risk Factors" section of this prospectus. The following is a summary of such risks:

● our ability to continue as a going concern;

● our planned online machine learning platform's ability to enable universities and other clients to offer timely, improved popular courses and certification programs, without becoming software tech companies;

● our planned online machine learning platform's ability to result in opportunistic incremental revenue for colleges, universities and other clients, and improved ability to garner state funds due to increased retention and graduation rates through use of machine learning and natural language processing;

● our ability to obtain additional funds for our operations;

● our ability to obtain and maintain intellectual property protection for our technologies and our ability to operate our business without infringing the intellectual property rights of others;

● our reliance on third parties to conduct our business and studies;

● our reliance on third party designers, suppliers, and Partners to provide and maintain our learning platform;

● our ability to attract and retain qualified key management and technical personnel;

● our expectations regarding the time during which we will be an emerging growth company under the Jumpstart Our Business Startups Act, or JOBS Act;

● our financial performance;

● the impact of government regulation and developments relating to our competitors or our industry; and

● other risks and uncertainties, including those listed under the caption "Risk Factors."

These statements relate to future events or our future operational or financial performance, and involve known and unknown risks, uncertainties and other factors that may cause our actual results, performance or achievements to be materially different from any future results, performance or achievements expressed or implied by these forward-looking statements. Factors that may cause actual results to differ materially from current expectations include, among other things, those listed under the section titled "Risk Factors" and elsewhere in this prospectus.

Any forward-looking statement in this prospectus reflects our current view with respect to future events and is subject to these and other risks, uncertainties and assumptions relating to our business, results of operations, industry and future growth. Given these uncertainties, you should not place undue reliance on these forward-looking statements. No forward-looking statement is a guarantee of future performance. You should read this prospectus, and the documents that we reference herein and have filed as exhibits hereto completely and with the understanding that our actual future results may be materially different from any future results expressed or implied by these forward-looking statements. Except as required by law, we assume no obligation to update or revise these forward-looking statements for any reason, even if new information becomes available in the future

This prospectus also contains, or may contain, estimates, projections and other information concerning our industry, our business and the markets for our products, including data regarding the estimated size of those markets and their projected growth rates. Information that is based on estimates, forecasts, projections or similar methodologies is inherently subject to uncertainties and actual events or circumstances may differ materially from events and circumstances reflected in this information. Unless otherwise expressly stated, we obtained these industry, business, market and other data from reports, research surveys, studies and similar data prepared by third parties, industry and general publications, government data and similar sources. In some cases, we do not expressly refer to the sources from which these data are derived.

**USE OF PROCEEDS**

We are not selling any shares of our common stock in this offering and we will not receive any of the proceeds from the sale of shares of our common stock by the selling stockholders. The selling stockholders will receive all of the proceeds from any sales of the shares of our common stock offered hereby. However, we will incur expenses in connection with the registration of the shares of our common stock offered hereby.

We will receive the exercise price upon any exercise of the warrants, to the extent exercised on a cash basis. If all the warrants were exercised for cash, we would receive gross proceeds of approximately $3.6 million. However, the holders of the warrants are not obligated to exercise the warrants, and we cannot predict whether or when, if ever, the holders of the warrants will choose to exercise the warrants, in whole or in part. Accordingly, any proceeds from such exercise will be used for general corporate purposes and working capital.

**MARKET FOR COMMON STOCK AND DIVIDEND POLICY**

Our common stock is traded on the Nasdaq Capital Market under the symbol "AMST." The last reported sale price of our common stock on March 10, 2023 on the Nasdaq Capital Market was $2.80 per share. As of March 10, 2023, there were 39 stockholders of record of our common stock.

We have never declared or paid any cash dividend on our common stock. We intend to retain any future earnings and do not expect to pay dividends in the foreseeable future.

**SECURITY OWNERSHIP OF BENEFICIAL OWNERS AND MANAGEMENT**

The following table sets forth certain information regarding beneficial ownership of shares of our common stock as of March 10, 2023, based on 2,533,359 shares of common stock and 100,000 shares of Series A preferred stock issued and outstanding by (i) each person known to beneficially own more than 5% of our outstanding common stock, (ii) each of our directors and director nominees, (iii) our named executive officers and (iv) all directors and executive officers as a group. Shares are beneficially owned when an individual has voting and/or investment power over the shares or could obtain voting and/or investment power over the shares within 60 days of the March 10, 2023. Except as otherwise indicated, the persons named in the table have sole voting and investment power with respect to all shares beneficially owned, subject to community property laws, where applicable. Unless otherwise indicated, the address of each beneficial owner listed below is c/o Amesite Inc., 607 Shelby Street, Suite 700 PMB 214, Detroit, Michigan 48226.

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| | | |
|:---|:---|:---|
| **Name of Beneficial Owner and Title of Officers and Directors** | **Shares of Common Stock Beneficially Owned** | **Percentage** |
| Ann Marie Sastry, Ph.D., President, Chief Executive Officer, and Chairman of the Board <sup>(1)</sup> | 575848 | 22.3% |
| Sherlyn W. Farrell, Chief Financial Officer |  | \* |
| J. Michael Losh, Director <sup>(2)</sup> | 37587 | 1.5% |
| Gilbert S. Omenn, M.D., Ph.D., Director <sup>(3)</sup> | 21510 | \*% |
| Richard T. Ogawa, Director <sup>(4)</sup> | 57726 | 2.2% |
| Anthony M. Barkett, Director <sup>(5)</sup> | 31493 | 1.2% |
| Barbie Brewer, Director <sup>(6)</sup> | 27083 | 1.1% |
| George Parmer, Director | 84167 | 3.3% |
| **All Officers and Directors as a Group (8 persons) <sup>(7)</sup>** | **835414** | 30.6% |
| **Beneficial Owner Greater than 5% Stockholders** |  |  |
| Mark Tompkins <sup>(8)</sup> | 170259 | 6.7% |

---

\* Less than 1%.

(1) Includes (i) 532,098 shares of common stock held by Dr. Sastry and (ii)
43,750 shares of common stock underlying options that are presently exercisable or exercisable within 60 days of March 10, 2023 held by
Dr. Sastry.

(2) Includes (i) 3,472 shares of common stock held by Mr. Losh and (ii) 34,115 shares of common stock underlying options that are presently exercisable or exercisable within 60 days of March 10, 2023 held by Mr. Losh.

(3) Includes (i) 3,472 shares of common stock held by Dr. Omenn and (ii) 18,038 shares of common stock underlying options that are presently exercisable or exercisable within 60 days of March 10, 2023 held by Dr. Omenn.

(4) Includes (i) 5,556 shares of common stock held by Mr. Ogawa and (ii) 52,170 shares of common stock underlying options that are presently exercisable or exercisable within 60 days of March 10, 2023 held by Mr. Ogawa.

(5) Includes (i) 4,167 shares of common stock held by Mr. Barkett and (ii) 27,326 shares of common stock underlying options that are presently exercisable or exercisable within 60 days of March 10, 2023 held by Mr. Barkett.

(6) Includes (i) 2,083 shares of common stock held by Ms. Brewer and (ii) 27,083 shares of common stock underlying options that are presently exercisable or exercisable within 60 days of March 10, 2023 held by Ms. Brewer.

(7) Includes 200,399 shares of common stock underlying options that are either presently exercisable or exercisable within 60 days of March 10, 2023 held by all directors and officers as a group.

(8) Mr. Tompkins's address is Apt 1, via Guidino 23, 6900 Lugano, Paradiso, Switzerland. Mr. Tompkins has voting and dispositive authority over the shares.

**SELLING STOCKHOLDERS**

The common stock being offered by the selling stockholders are those issuable to the selling stockholders upon exercise of the warrants. For additional information regarding the issuance of the warrants, see "The Private Placement" above. We are registering the shares of common stock in order to permit the selling stockholders to offer the shares for resale from time to time. Except as described below, to our knowledge, none of the selling stockholders has been an officer or director of ours or of our affiliates within the past three years or has any material relationship with us or our affiliates within the past three years. Our knowledge is based on information provided by the selling stockholders in connection with the filing of this prospectus.

The table below lists the selling stockholders and other information regarding the beneficial ownership of the shares of common stock by each of the selling stockholders. The second column lists the number of shares of common stock beneficially owned by each selling stockholder, based on its ownership of the shares of common stock, options to purchase common stock, and warrants, as of March 10, 2023, assuming exercise of the warrants held by the selling stockholders on that date, without regard to any limitations on exercises. The third column lists the maximum number of shares of common stock that may be sold or otherwise disposed of by the selling stockholders pursuant to the registration statement of which this prospectus forms a part. The selling stockholders may sell or otherwise dispose of some, all or none of their shares. Pursuant to Rules 13d-3 and 13d-5 of the Exchange Act, beneficial ownership includes any shares of our common stock as to which a stockholders has sole or shared voting power or investment power, and also any shares of our common stock which the stockholder has the right to acquire within 60 days of March 10, 2023. The percentage of beneficial ownership for the selling stockholders is based on 2,533,359 shares of our common stock outstanding as of March 10, 2023 and the number of shares of our common stock issuable upon exercise or conversion of convertible securities that are currently exercisable or convertible or are exercisable or convertible within 60 days of March 10, 2023 beneficially owned by the applicable selling stockholder. The fourth column assumes the sale of all of the shares of common stock offered by the selling stockholders pursuant to this prospectus.

Under the terms of the warrants, a selling stockholder may not exercise the warrants to the extent such exercise would cause such selling stockholder, together with its affiliates and attribution parties, to beneficially own a number of shares of common stock which would exceed 4.99% (or for certain holders, 9.99%) of our then outstanding common stock following such exercise, excluding for purposes of such determination shares of common stock issuable upon exercise of the warrants which have not been exercised. The number of shares in the second column does not reflect this limitation. The selling stockholders may sell all, some or none of their shares in this offering. See "Plan of Distribution."

Information about the selling stockholders may change over time. Any changed information will be set forth in an amendment to the registration statement or supplement to this prospectus, to the extent required by law. Unless otherwise noted below, the address of each selling stockholder listed on the table is c/o Amesite Inc., 607 Shelby Street, Suite 700 PMB 214, Detroit, MI 48226.

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| | | | | | |
|:---|:---|:---|:---|:---|:---|
| | **Beneficial Ownership Prior to the Offering(1)** | **Beneficial Ownership Prior to the Offering(1)** | | **Beneficial Ownership After the Offering(2)** | **Beneficial Ownership After the Offering(2)** |
| <br>**Name of Selling Stockholder** | **Number of<br> Shares of<br> Common<br> Stock<br> Beneficially<br> Owned Prior<br> to the<br> Offering** | **Percentage<br> of<br> Outstanding<br> Common<br> Stock(2)** | **Maximum**<br>**Number of<br> Shares of<br> Common<br> Stock<br> To Be Sold<br> Pursuant<br> to this<br> Prospectus** | **Number of<br> Shares of<br> Common<br> Stock<br> Beneficially<br> Owned<br> After the<br> Offering** | **Percentage of<br> Outstanding<br> Common<br> Stock(2)** |
| Donald Garlikov | 181819 | 7.2% | 91660 | 90159 | 3.6% |
| CVI Investment, Inc. | 90910 | 3.6% | 45455 | 45455 | 1.8% |
| 3I, LP | 75758 | 3.0% | 37879 | 37879 | 1.5% |
| Lincoln Park Capital, LLC | 75758 | 3.0% | 37879 | 37879 | 1.5% |
| Warberg WFX LP | 75758 | 3.0% | 37879 | 37879 | 1.5% |
| Iroquois Capital Investment Group | 54538 | 2.2% | 27273 | 27265 | 1.1% |
| Iroquois Master Fund Ltd. | 36364 | 1.4% | 18182 | 18182 | \* |
| Evergreen Capital Management | 30303 | 1.2% | 15152 | 15151 | \* |
| Akita Partners, LLC | 22728 | \* | 11364 | 11364 | \* |
| KBB Asset Management | 22728 | \* | 11364 | 11364 | \* |
| Boothbay Diversified Alpha Master Fund LP | 20073 | \* | 10037 | 10036 | \* |
| Laidlaw & Co (UK) Ltd. | 17425 | \* | 17425 |  | \* |
| Boothbay Absolute Return Strategies, LP | 10231 | \* | 5116 | 5115 | \* |

---

\* Represents less than 1%.

(1) Assumes all warrants are exercised.

(2) Assumes that (i) all of the shares of common stock to be registered by the registration statement of which this prospectus is a part are sold in this offering and (ii) the selling stockholders do not acquire additional shares of our common stock after the date of this prospectus and prior to completion of this offering. The percentage of beneficial ownership after the offering is based on 2,900,024 shares of common stock, consisting of (a) 2,533,359 shares of our common stock outstanding on March 10, 2023, and (b) the 366,665 shares of our common stock underlying the warrants offered under this prospectus. The number of shares listed do not take into account any limitations on exercise of the warrants.

**PLAN OF DISTRIBUTION**

Each selling stockholder of the securities and any of their pledgees, assignees and successors-in-interest may, from time to time, sell any or all of their securities covered hereby on The Nasdaq Capital Market or any other stock exchange, market or trading facility on which the securities are traded or in private transactions. These sales may be at fixed or negotiated prices. A selling stockholder may use any one or more of the following methods when selling securities:

● ordinary brokerage transactions and transactions in which the broker-dealer solicits purchasers;

● block trades in which the broker-dealer will attempt to sell the securities as agent but may position and resell a portion of the block as principal to facilitate the transaction;

● purchases by a broker-dealer as principal and resale by the broker-dealer for its account;

● an exchange distribution in accordance with the rules of the applicable exchange;

● privately negotiated transactions;

● settlement of short sales;

● in transactions through broker-dealers that agree with the selling stockholders to sell a specified number of such securities at a stipulated price per security;

● through the writing or settlement of options or other hedging transactions, whether through an options exchange or otherwise;

● a combination of any such methods of sale; or

● any other method permitted pursuant to applicable law.

The selling stockholders may also sell securities under Rule 144 or any other exemption from registration under the Securities Act of 1933, as amended (the "Securities Act"), if available, rather than under this prospectus.

Broker-dealers engaged by the selling stockholders may arrange for other brokers-dealers to participate in sales. Broker-dealers may receive commissions or discounts from the selling stockholders (or, if any broker-dealer acts as agent for the purchaser of securities, from the purchaser) in amounts to be negotiated, but, except as set forth in a supplement to this Prospectus, in the case of an agency transaction not in excess of a customary brokerage commission in compliance with FINRA Rule 2121; and in the case of a principal transaction a markup or markdown in compliance with FINRA Rule 2121.

In connection with the sale of the securities or interests therein, the selling stockholders may enter into hedging transactions with broker-dealers or other financial institutions, which may in turn engage in short sales of the securities in the course of hedging the positions they assume. The selling stockholders may also sell securities short and deliver these securities to close out their short positions, or loan or pledge the securities to broker-dealers that in turn may sell these securities. The selling stockholders may also enter into option or other transactions with broker-dealers or other financial institutions or create one or more derivative securities which require the delivery to such broker-dealer or other financial institution of securities offered by this prospectus, which securities such broker-dealer or other financial institution may resell pursuant to this prospectus (as supplemented or amended to reflect such transaction).

The selling stockholders and any broker-dealers or agents that are involved in selling the securities may be deemed to be "underwriters" within the meaning of the Securities Act in connection with such sales. In such event, any commissions received by such broker-dealers or agents and any profit on the resale of the securities purchased by them may be deemed to be underwriting commissions or discounts under the Securities Act. Each selling stockholder has informed the Company that it does not have any written or oral agreement or understanding, directly or indirectly, with any person to distribute the securities.

The Company is required to pay certain fees and expenses incurred by the Company incident to the registration of the securities. The Company has agreed to indemnify the selling stockholders against certain losses, claims, damages and liabilities, including liabilities under the Securities Act.

We agreed to use commercially reasonable efforts to keep this registration statement effective at all times until the selling stockholders no longer own any Warrants or shares of Common Stock issuable upon the exercise of the Warrants.

Under applicable rules and regulations under the Exchange Act, any person engaged in the distribution of the resale securities may not simultaneously engage in market making activities with respect to the Common Stock for the applicable restricted period, as defined in Regulation M, prior to the commencement of the distribution. In addition, the selling stockholders will be subject to applicable provisions of the Exchange Act and the rules and regulations thereunder, including Regulation M, which may limit the timing of purchases and sales of the Common Stock by the selling stockholders or any other person. We will make copies of this prospectus available to the selling stockholders and have informed them of the need to deliver a copy of this prospectus to each purchaser at or prior to the time of the sale (including by compliance with Rule 172 under the Securities Act).

**DESCRIPTION OF OUR SECURITIES TO BE REGISTERED**

The securities to be registered on this registration statement on Form S-1 include up to an aggregate amount of 366,665 shares of common stock, consisting of (i) up to 349,240 shares of our common stock issuable upon exercise of the warrants acquired by certain of the selling stockholders under the Purchase Agreements, and (ii) up to 17,425 shares of our common stock issuable upon exercise of placement agent warrants acquired by certain of the selling stockholders under the Placement Agent Agreement.

**General**

The following is a summary of material characteristics of our capital stock as set forth in our certificate of incorporation and bylaws, and certain provisions of Delaware law. The following description does not purport to be complete and is subject to and qualified in its entirety by, and should be read in conjuncture with, our certificate of incorporation and bylaws, each of which are filed as exhibits to this Registration Statement and are incorporated herein by reference. The summaries and descriptions below do not purport to be complete statements of the Delaware General Corporation Law ("DGCL").

The Company is authorized to issue 105,000,000 shares of capital stock, par value $0.0001 per share, of which 100,000,000 are shares of common stock and 5,000,000 are shares of "blank check" preferred stock.

As of the date of this prospectus, there were 2,533,359 shares of our common stock issued and outstanding and no shares of preferred stock issued and outstanding.

***Common Stock***

*Voting*

The holders of our common stock are entitled to one vote for each share held on all matters to be voted on by the Company's stockholders. There shall be no cumulative voting.

*Dividends*

The holders of shares of our common stock are entitled to dividends when and as declared by the Board from funds legally available therefor if, as and when determined by the Board of Directors of the Company in their sole discretion, subject to provisions of law, and any provision of the Company's Certificate of Incorporation, as amended from time to time. There are no preemptive, conversion or redemption privileges, nor sinking fund provisions with respect to the common stock.

*Liquidation*

In the event of any voluntary or involuntary liquidation, dissolution or winding up of our affairs, the holders of our common stock will be entitled to share ratably in the net assets legally available for distribution to stockholders after the payment of or provision for all of our debts and other liabilities.

*Fully Paid and Non-assessable*

All outstanding shares of common stock are duly authorized, validly issued, fully paid and non-assessable.

***Preferred Stock***

We are authorized to issue up to 5,000,000 shares of preferred stock. This preferred stock may be issued in one or more series, the terms of which may be determined at the time of issuance by our board of directors without further action by stockholders. The terms of any series of preferred stock may include voting rights (including the right to vote as a series on particular matters), preferences as to dividend, liquidation, conversion and redemption rights and sinking fund provisions. No preferred stock is currently outstanding. The issuance of any preferred stock could materially adversely affect the rights of the holders of our common stock, and therefore, reduce the value of our common stock and the Notes. In particular, specific rights granted to future holders of preferred stock could be used to restrict our ability to merge with, or sell our assets to, a third party and thereby preserve control by the present management.

*Series A Preferred Stock*

 ****

On January 23, 2023, we filed a Certificate of Designation of the Series A Preferred Stock (the "Certificate of Designation") with the Secretary of State of the State of Delaware to create a new class of Series A Preferred Stock, par value $0.0001 per share. The Certificate of Designation designates 100,000 shares of authorized preferred stock as Series A Preferred Stock. The Series A Preferred Stock are not entitled to receive dividends or any other distributions. The Series A Preferred Stock are entitled to one thousand votes per share and shall vote together with the issued and outstanding shares of our common stock as a single class exclusively with respect to the Reverse Stock Split (as defined in the Certificate of Designation). The Series A Preferred Stock have no rights as to any distribution or assets of the Company upon a liquidation, bankruptcy, reorganization, merger, acquisition, sale, dissolution or winding up of the Company. The outstanding shares of Series A Preferred Stock shall be redeemed in whole, but not in part for an aggregate price of $1,000 (i) if such redemption is ordered by our board of directors, in its sole discretion, or (ii) automatically and effective immediately after the effectiveness of the Reverse Stock Split.

**Exclusive Forum**

Our Certificate of Incorporation provides that unless the Company consents in writing to the selection of an alternative forum, the State of Delaware is the sole and exclusive forum for: (i) any derivative action or proceeding brought on behalf of the Company, (ii) any action asserting a claim of breach of a fiduciary duty owed by any director, officer or other employee of the Company to the Company or the Company's stockholders, (iii) any action asserting a claim against the Company, its directors, officers or employees arising pursuant to any provision of the DGCL or our Certificate of Incorporation or the Bylaws, or (iv) any action asserting a claim against the Company, its directors, officers, employees or agents governed by the internal affairs doctrine, except for, as to each of (i) through (iv) above, any claim as to which the Court of Chancery determines that there is an indispensable party not subject to the jurisdiction of the Court of Chancery (and the indispensable party does not consent to the personal jurisdiction of the Court of Chancery within ten days following such determination), which is vested in the exclusive jurisdiction of a court or forum other than the Court of Chancery, or for which the Court of Chancery does not have subject matter jurisdiction.

Additionally, our Certificate of Incorporation provide that unless the Company consents in writing to the selection of an alternative forum, the federal district courts of the United States of America will be the exclusive forum for the resolution of any complaint asserting a cause of action arising under the Securities Act. Any person or entity purchasing or otherwise acquiring any interest in shares of capital stock of the Corporation are deemed to have notice of and consented to this provision. The Supreme Court of Delaware has held that this type of exclusive federal forum provision is enforceable. There may be uncertainty, however, as to whether courts of other jurisdictions would enforce such a provision, if applicable.

**Transfer Agent**

The transfer agent and registrar for our common stock is Continental Stock Transfer & Trust Company.

**Changes in Authorized Number**

The Board of Directors is expressly authorized to increase or decrease the number of shares of any series subsequent to the issuance of shares of that series, but not below the number of shares of such series then outstanding. The number of authorized shares of Preferred Stock may be increased or decreased (but not below the number of shares thereof then outstanding) by the affirmative vote of the holders of a majority of the voting power of the stock of the Company entitled to vote thereon, without a separate vote of the holders of the Preferred Stock, or of any series thereof, unless a vote of any such holders is required pursuant to the terms of any Certificate of Designation filed with respect to any series of Preferred Stock.

**Delaware Anti-Takeover Statute**

We may become subject to Section 203 of the Delaware General Corporation Law, which prohibits persons deemed to be "interested stockholders" from engaging in a "business combination" with a publicly held Delaware corporation for three years following the date these persons become interested stockholders unless the business combination is, or the transaction in which the person became an interested stockholder was, approved in a prescribed manner or another prescribed exception applies. Generally, an "interested stockholder" is a person who, together with affiliates and associates, owns, or within three years prior to the determination of interested stockholder status did own, 15% or more of a corporation's voting stock. Generally, a "business combination" includes a merger, asset or stock sale, or other transaction resulting in a financial benefit to the interested stockholder. The existence of this provision may have an anti-takeover effect with respect to transactions not approved in advance by the Board of Directors. A Delaware corporation may "opt out" of these provisions with an express provision in its original certificate of incorporation or an express provision in its certificate of incorporation or bylaws resulting from a stockholders' amendment approved by at least a majority of the outstanding voting shares. We have not opted out of these provisions. As a result, mergers or other takeover or change in control attempts of us may be discouraged or prevented.

The Bylaws establish an advance notice procedure for stockholder proposals to be brought before an annual meeting of our stockholders, including proposed nominations of persons for election to our board of directors. At an annual meeting, stockholders may only consider proposals or nominations specified in the notice of meeting or brought before the meeting by or at the direction of our board of directors. Stockholders may also consider a proposal or nomination by a person who was a stockholder at the time of giving notice and at the time of the meeting, who is entitled to vote at the meeting and who has complied with the notice requirements of the Bylaws in all respects. The Bylaws do not give our board of directors the power to approve or disapprove stockholder nominations of candidates or proposals regarding other business to be conducted at a special or annual meeting of our stockholders. However, the Bylaws may have the effect of precluding the conduct of certain business at a meeting if the proper procedures are not followed. These provisions may also discourage or deter a potential acquirer from conducting a solicitation of proxies to elect the acquirer's own slate of directors or otherwise attempting to obtain control of our company.

The Bylaws provide that a special meeting of our stockholders may be called only by our Secretary at the direction of the Board or by resolution adopted by a majority of our board of directors. Because our stockholders do not have the right to call a special meeting, a stockholder could not force stockholder consideration of a proposal over the opposition of our board of directors by calling a special meeting of stockholders prior to such time as a majority of our board of directors or the Secretary believe the matter should be considered or until the next annual meeting *provided* that the requestor met the notice requirements. The restriction on the ability of stockholders to call a special meeting means that a proposal to replace our board of directors also could be delayed until the next annual meeting.

**Limitations on Liability and Indemnification of Officers and Directors**

We intend to enter into separate indemnification agreements with each of our directors and executive officers. Each indemnification agreement will provide, among other things, for indemnification to the fullest extent permitted by law and our Certificate of Incorporation and Bylaws against any and all expenses, judgments, fines, penalties and amounts paid in settlement of any claim. The indemnification agreements will provide for the advancement or payment of all expenses to the indemnitee and for the reimbursement to us if it is found that such indemnitee is not entitled to such indemnification under applicable law and our Certificate of Incorporation and Bylaws.

We maintain a general liability insurance policy that covers certain liabilities of directors and officers of our corporation arising out of claims based on acts or omissions in their capacities as directors or officers.

**Section 203 of the Delaware General Corporation Law**

We may become subject to Section 203 of the Delaware General Corporation Law, which prohibits persons deemed to be "interested stockholders" from engaging in a "business combination" with a publicly held Delaware corporation for three years following the date these persons become interested stockholders unless the business combination is, or the transaction in which the person became an interested stockholder was, approved in a prescribed manner or another prescribed exception applies. Generally, an "interested stockholder" is a person who, together with affiliates and associates, owns, or within three years prior to the determination of interested stockholder status did own, 15% or more of a corporation's voting stock. Generally, a "business combination" includes a merger, asset or stock sale, or other transaction resulting in a financial benefit to the interested stockholder. The existence of this provision may have an anti-takeover effect with respect to transactions not approved in advance by the Board of Directors. A Delaware corporation may "opt out" of these provisions with an express provision in its original certificate of incorporation or an express provision in its certificate of incorporation or bylaws resulting from a stockholders' amendment approved by at least a majority of the outstanding voting shares. We have not opted out of these provisions. As a result, mergers or other takeover or change in control attempts of us may be discouraged or prevented.

The Bylaws establish an advance notice procedure for stockholder proposals to be brought before an annual meeting of our stockholders, including proposed nominations of persons for election to our board of directors. At an annual meeting, stockholders may only consider proposals or nominations specified in the notice of meeting or brought before the meeting by or at the direction of our board of directors. Stockholders may also consider a proposal or nomination by a person who was a stockholder at the time of giving notice and at the time of the meeting, who is entitled to vote at the meeting and who has complied with the notice requirements of the Bylaws in all respects. The Bylaws do not give our board of directors the power to approve or disapprove stockholder nominations of candidates or proposals regarding other business to be conducted at a special or annual meeting of our stockholders. However, the Bylaws may have the effect of precluding the conduct of certain business at a meeting if the proper procedures are not followed. These provisions may also discourage or deter a potential acquirer from conducting a solicitation of proxies to elect the acquirer's own slate of directors or otherwise attempting to obtain control of our company.

The Bylaws provide that a special meeting of our stockholders may be called only by our Secretary at the direction of the Board or by resolution adopted by a majority of our board of directors. Because our stockholders do not have the right to call a special meeting, a stockholder could not force stockholder consideration of a proposal over the opposition of our board of directors by calling a special meeting of stockholders prior to such time as a majority of our board of directors or the Secretary believe the matter should be considered or until the next annual meeting *provided* that the requestor met the notice requirements. The restriction on the ability of stockholders to call a special meeting means that a proposal to replace our board of directors also could be delayed until the next annual meeting.

**Listing**

Our common stock is listed on The Nasdaq Capital Market under the symbol "AMST."

**LEGAL MATTERS**

Sheppard, Mullin, Richter & Hampton LLP, New York, New York, will pass upon the validity of the issuance of the securities to be offered by this prospectus.

**EXPERTS**

The financial statements of Amesite Inc. as of June 30, 2022 and 2021, and for each of the two years in the period ended June 30, 2022, incorporated by reference in this Prospectus, have been audited by Deloitte & Touche LLP, an independent registered public accounting firm, as stated in their report. Such financial statements are incorporated by reference in reliance upon the report of such firm given their authority as experts in accounting and auditing.

**WHERE YOU CAN FIND MORE INFORMATION**

We have filed a registration statement on Form S-1 with respect to the shares of common stock offered by this prospectus with the SEC in accordance with the Securities Act and the rules and regulations enacted under its authority. This prospectus, which constitutes a part of the registration statement, does not contain all of the information included in the registration statement and its exhibits and schedules. Any statement made in this prospectus concerning the contents of any contract, agreement or other document is only a summary of the actual contract, agreement or other document. If we have filed or incorporated by reference any contract, agreement or other document as an exhibit to the registration statement, you should read the exhibit for a more complete understanding of the document or matter involved. Each statement regarding a contract, agreement or other document is qualified by reference to the actual document. For further information regarding us and the shares of common stock offered by this prospectus, we refer you to the full registration statement, including its exhibits and schedules, filed under the Securities Act.

The SEC maintains a website at http://www.sec.gov that contains reports, proxy and information statements and other information regarding issuers that file electronically with the SEC. Our registration statement, of which this prospectus constitutes a part, can be downloaded from the SEC's website.

We also file annual, quarterly and current reports, proxy statements and other information with the SEC. You can read our SEC filings on the SEC's website at http://www.sec.gov.

Our website address is https://amesite.com/. There we make available free of charge, on or through the investor relations section of our website, annual reports on Form 10-K, quarterly reports on Form 10-Q, current reports on Form 8-K and amendments to those reports filed pursuant to Section 13(a) or 15(d) of the Exchange Act as soon as reasonably practicable after we electronically file such material with the SEC. The information contained on, or that can be accessed through, our website is not a part of this prospectus, and our reference to the address for our website is intended to be an inactive textual reference only.

**INCORPORATION OF DOCUMENTS BY REFERENCE**

The rules of the SEC allow us to incorporate by reference into this prospectus the information we file with the SEC. This means that we are disclosing important information to you by referring to other documents. The information incorporated by reference is considered to be part of this prospectus, except for any information superseded by information contained directly in this prospectus. We incorporate by reference the documents listed below (other than any portions thereof, which under the Exchange Act, and applicable SEC rules, are not deemed "filed" under the Exchange Act):

● our Annual Report on [Form 10-K](http://www.sec.gov/ix?doc=/Archives/edgar/data/0001807166/000121390022059740/f10k2022_amesiteinc.htm) for the fiscal year ended June 30, 2022, filed with the SEC on September 28, 2022 <u>;</u> 

● our Quarterly Report on [Form 10-Q](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390022071308/f10q0922_amesiteinc.htm) for the fiscal quarter ended September 30, 2022, filed with the SEC on November 10, 2022;

● our Quarterly Report on [Form 10-Q](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390023012931/f10q1222_amesiteinc.htm) for the fiscal quarter ended December 31, 2022, filed with the SEC on February 17, 2023;

● our Current Reports on Form 8-K, filed with the SEC on [December 15, 2022](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390022080131/ea170290-8k_amesiteinc.htm) , [December 16, 2022](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390022080499/ea170349-8k_amesiteinc.htm) , [January 13, 2023,](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390023002835/ea171681-8k_amesiteinc.htm) [February 2, 2023](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390023007353/ea172576-8k_amesite.htm) and [February 21, 2023](http://www.sec.gov/ix?doc=/Archives/edgar/data/1807166/000121390023013310/ea173775-8k_amesite.htm) ; and

● our Proxy Statement on [Schedule 14A](http://www.sec.gov/Archives/edgar/data/1807166/000121390022067190/ea167690-def14a_amesiteinc.htm) filed on October 28, 2022; and

● the description of our common stock contained in our Registration Statement on September 23, 2020, including any amendment or report filed for the purpose of updating such description.

The SEC file number for each of the documents listed above is 001-39553.

In addition, all documents subsequently filed by us with the SEC pursuant to Sections 13(a), 13(c), 14 or 15(d) of the Exchange Act, prior to the termination of the offering, shall be deemed to be incorporated by reference into this prospectus; provided, however, that all reports, exhibits and other information that we "furnish" to the SEC will not be considered incorporated by reference into this prospectus. If we have incorporated by reference any statement or information in this prospectus and we subsequently modify that statement or information with information contained in this prospectus, the statement or information previously incorporated in this prospectus is also modified or superseded in the same manner.

You may request, orally or in writing, a copy of any or all of the documents incorporated herein by reference. These documents will be provided to you at no cost, by contacting:

Amesite Inc.<br> 607 Shelby Street

Suite 700 PMB 214

Detroit, MI

(734) 876-8130

You may also access these documents on our website, https://amesite.com/ The information contained on, or that can be accessed through, our website is not a part of this prospectus. We have included our website address in this prospectus solely as an inactive textual reference.

You should rely only on information contained in, or incorporated by reference into, this prospectus and any prospectus supplement. We have not authorized anyone to provide you with information different from that contained in this prospectus or incorporated by reference in this prospectus. We are not making offers to sell the securities in any jurisdiction in which such an offer or solicitation is not authorized or in which the person making such offer or solicitation is not qualified to do so or to anyone to whom it is unlawful to make such offer or solicitation.

**PART II**

**INFORMATION NOT REQUIRED IN PROSPECTUS**

**ITEM 13.**

**Other Expenses of Issuance and Distribution.**

The following table sets forth the costs and expenses, payable by the Company in connection with the registration and sale of the Common Stock being registered. All amounts are estimates except the SEC registration fee.

---

| | |
|:---|:---|
|  | **Amount** |
| SEC registration fee | $484 |
| Accounting fees and expenses | 25000 |
| Legal fees and expenses | 15000 |
| Miscellaneous | 1000 |
| Total expenses | $41484 |

---

**ITEM 14. Indemnification of Directors and Officers.**

Section 102 of the General Corporation Law of the State of Delaware (the "DGCL") permits a corporation to eliminate the personal liability of directors of a corporation to the corporation or its stockholders for monetary damages for a breach of fiduciary duty as a director, except where the director breached his duty of loyalty, failed to act in good faith, engaged in intentional misconduct or knowingly violated a law, authorized the payment of a dividend or approved a stock repurchase in violation of Delaware corporate law or obtained an improper personal benefit. Our Certificate of Incorporation provides that no director of the Company shall be personally liable to it or its stockholders for monetary damages for any breach of fiduciary duty as a director, notwithstanding any provision of law imposing such liability, except to the extent that the DGCL prohibits the elimination or limitation of liability of directors for breaches of fiduciary duty.

Section 145 of the DGCL provides that a corporation has the power to indemnify a director, officer, employee, or agent of the corporation, or a person serving at the request of the corporation for another corporation, partnership, joint venture, trust or other enterprise in related capacities against expenses (including attorneys' fees), judgments, fines and amounts paid in settlement actually and reasonably incurred by the person in connection with an action, suit or proceeding to which he was or is a party or is threatened to be made a party to any threatened, ending or completed action, suit or proceeding by reason of such position, if such person acted in good faith and in a manner he reasonably believed to be in or not opposed to the best interests of the corporation, and, in any criminal action or proceeding, had no reasonable cause to believe his conduct was unlawful, except that, in the case of actions brought by or in the right of the corporation, no indemnification shall be made with respect to any claim, issue or matter as to which such person shall have been adjudged to be liable to the corporation unless and only to the extent that the Court of Chancery or other adjudicating court determines that, despite the adjudication of liability but in view of all of the circumstances of the case, such person is fairly and reasonably entitled to indemnity for such expenses which the Court of Chancery or such other court shall deem proper.

Our Certificate of Incorporation and Bylaws provide indemnification for our directors and officers to the fullest extent permitted by the DGCL. We will indemnify each person who was or is a party or threatened to be made a party to any threatened, pending or completed action, suit or proceeding (other than an action by or in the right of us) by reason of the fact that he or she is or was, or has agreed to become, a director or officer, or is or was serving, or has agreed to serve, at our request as a director, officer, partner, employee or trustee of, or in a similar capacity with, another corporation, partnership, joint venture, trust or other enterprise (all such persons being referred to as an "Indemnitee"), or by reason of any action alleged to have been taken or omitted in such capacity, against all expenses (including attorneys' fees), judgments, fines and amounts paid in settlement actually and reasonably incurred in connection with such action, suit or proceeding and any appeal therefrom, if such Indemnitee acted in good faith and in a manner he or she reasonably believed to be in, or not opposed to, our best interests, and, with respect to any criminal action or proceeding, he or she had no reasonable cause to believe his or her conduct was unlawful. Our Certificate of Incorporation and Bylaws provide that we will indemnify any Indemnitee who was or is a party to an action or suit by or in the right of us to procure a judgment in our favor by reason of the fact that the Indemnitee is or was, or has agreed to become, a director or officer, or is or was serving, or has agreed to serve, at our request as a director, officer, partner, employee or trustee of, or in a similar capacity with, another corporation, partnership, joint venture, trust or other enterprise, or by reason of any action alleged to have been taken or omitted in such capacity, against all expenses (including attorneys' fees) and, to the extent permitted by law, amounts paid in settlement actually and reasonably incurred in connection with such action, suit or proceeding, and any appeal therefrom, if the Indemnitee acted in good faith and in a manner he or she reasonably believed to be in, or not opposed to, our best interests, except that no indemnification shall be made with respect to any claim, issue or matter as to which such person shall have been adjudged to be liable to us, unless a court determines that, despite such adjudication but in view of all of the circumstances, he or she is entitled to indemnification of such expenses. Notwithstanding the foregoing, to the extent that any Indemnitee has been successful, on the merits or otherwise, he or she will be indemnified by us against all expenses (including attorneys' fees) actually and reasonably incurred in connection therewith. Expenses must be advanced to an Indemnitee under certain circumstances.

We intend to enter into separate indemnification agreements with each of our directors and executive officers. Each indemnification agreement will provide, among other things, for indemnification to the fullest extent permitted by law and our Certificate of Incorporation and Bylaws against any and all expenses, judgments, fines, penalties and amounts paid in settlement of any claim. The indemnification agreements will provide for the advancement or payment of all expenses to the indemnitee and for the reimbursement to us if it is found that such indemnitee is not entitled to such indemnification under applicable law and our Certificate of Incorporation and Bylaws.

We maintain a general liability insurance policy that covers certain liabilities of directors and officers of our corporation arising out of claims based on acts or omissions in their capacities as directors or officers.

**ITEM 15. Recent Sales of Unregistered Securities.**

*Private Placement with Lincoln Park Capital Fund, LLC*

On August 2, 2021, we entered into a purchase agreement (the "Purchase Agreement"), with Lincoln Park Capital Fund, LLC ("Lincoln Park"), under which, subject to specified terms and conditions, we may sell to Lincoln Park up to $16.5 million of shares of common stock, par value $0.0001 per share, from time to time during the term of the Purchase Agreement.

In connection with the Purchase Agreement, the Company entered into an introducing broker agreement with Laidlaw & Company (UK) Ltd. ("Laidlaw"), pursuant to which we agreed to pay a cash fee to Laidlaw (the "Introductory Fee") equal to (i) 8% of the amount of the Initial Purchase (ii) 8% of the amount of Tranche Purchase if any, and (iii) 4% of up to the next $13,500,000 (or up to $14,500,000 if the Tranche Purchase is not exercised).

Upon entering into the Purchase Agreement, the Company sold 759,109 shares of common stock to Lincoln Park as an initial purchase for a total purchase price of $1,500,000 (the "Initial Purchase"). The Company received net proceeds from the Initial Purchase of $1,380,000 after the payment of the Introductory Fee. As consideration for Lincoln Park's commitment to purchase up to $16.5 million of shares of common stock under the Purchase Agreement, the Company issued 152,715 shares of common stock to Lincoln Park.

*Private Placement with Laidlaw & Co (UK), Ltd.*

On August 30, 2022, the Company entered into a Securities Purchase Agreement (the "Purchase Agreement") with certain investors (the "Investors") for the purpose of raising approximately $2.3 million in gross proceeds for the Company (the "Offering"). Pursuant to the terms of the Purchase Agreement, the Company agreed to sell, in a registered direct offering, an aggregate of 4,181,821 shares (the "Shares") of the Company's common stock, par value $0.0001 per share (the "Common Stock"), and in a concurrent private placement, warrants to purchase an aggregate of 4,181,821 shares (the "Warrant Shares") of Common Stock (the "Warrants" and together with the Shares and the Warrant Shares, the "Securities"), for a combined purchase price per Share and Warrant of $0.55 (the "Purchase Price"). The Warrants are exercisable commencing six months after the date of their issuance, have an exercise price of $0.82 per share and expire five and one-half years from the date of issuance.

*Private Placement of Series A Preferred Stock*

On January 13, 2023, we entered into a Subscription and Investment Representation Agreement (the "Subscription Agreement") with George Parmer, a member of our Board of Directors (the "Purchaser") pursuant to which we issued and sold 100,000 shares (the "Shares") of our newly designated Series A Preferred Stock, par value $0.0001 per share (the "Series A Preferred Stock"), to such Purchaser for an aggregate purchase price of $1,000.

**Service-Related Issuances**

During the year ended June 30, 2022, 129,024 options to purchase common stock were issued to employees under our 2018 Equity Incentive Plan.

On October 19, 2021, the Company issued 9,901 shares of its common stock totaling approximately $18,218 to various consultants in exchange for strategic investor relations services. These shares vested immediately upon issuance.

On December 2, 2021, the Company issued 4,000 shares of its common stock totaling approximately $4,480 in value to various consultants in exchange for strategic investor relations services. These shares vested immediately upon issuance.

On May 20, 2022 and June 24, 2022, the Company issued 125,000 shares, respectively, of its common stock totaling approximately $126,250 in value to a consultant in exchange for strategic advisory and digital marketing services. These shares vested immediately upon issuance.

During the year ended June 30, 2021, 482,000 options to purchase common stock were issued to employees under our 2018 Equity Incentive Plan.

On November 3, 2020 the Company issued 69,709 shares of its common stock totaling approximately $290,000 to various consulting firms in exchange for strategic investor relations services. These shares vested immediately upon issuance.

On December 14, 2020, the Company issued 106,383 shares of its common stock totaling approximately $500,000 in value, respectively, to various consulting firms in exchange for strategic investor relations services. These shares vested immediately upon issuance.

In June 2021, the Company issued an additional 39,437 shares of its common stock totaling approximately $220,000 to a consulting firm for strategic investor relations services. These shares vested immediately upon issuance.

The foregoing issuances were exempt from registration under Section 4(a)(2) of the Securities Act.

**Item 16. Exhibits**

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) The following exhibits are filed herewith or incorporated herein by reference:

**EXHIBIT INDEX**

---

| | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|
| | | | **Incorporated by Reference** | **Incorporated by Reference** | **Incorporated by Reference** | **Incorporated by Reference** |
| **Exhibit**<br>**Number** | <br>**Exhibit Title** | **Filed<br> with this**<br>**Form S-1** | **Form** | **File No.** | **Exhibit** | **Date Filed** |
| 2.1\* | [Agreement and Plan of Merger and Reorganization, dated April 26, 2018, by and among Lola One Acquisition Corporation, Lola One Acquisition Sub, Inc., and Amesite Inc.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex2-1_amesite.htm) |  | S-1 | 333-248801 | 2.1 | 9/4/2020 |
| 2.2 | [Form of Agreement and Plan of Merger and Reorganization, dated July 14, 2020, by and between Amesite Operating Company, a Delaware corporation, and Amesite Inc., a Delaware corporation](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex2-2_amesite.htm) |  | S-1 | 333-248801 | 2.2 | 9/4/2020 |
| 3.1 | [Certificate of Merger of Lola One Acquisition Sub, Inc. with and into Amesite OpCo (then known as Amesite Inc.)](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex3-1_amesite.htm) |  | S-1 | 333-248802 | 3.1 | 9/4/2020 |
| 3.2 | [Form of Certificate of Merger relating to the merger of Amesite Inc. with and into Amesite Operating Company, to be filed with the Secretary of State of the State of Delaware.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex3-2_amesite.htm) |  | S-1 | 333-248801 | 3.2 | 9/4/2020 |
| 3.3 | [Amended and restated Bylaws, to be in effect after the completion of the Reorganization.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex3-7_amesite.htm) |  |  | 333-248801 | 3.7 | 9/4/2020 |
| 3.4 | [Certificate of Incorporation of the Registrant.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020037381/f10q0920ex3-1_amesiteinc.htm) |  | 10-Q |  | 3.1 | 11/16/2020 |
| 3.5 | [Bylaws of the Registrant](http://www.sec.gov/Archives/edgar/data/1807166/000121390020037381/f10q0920ex3-2_amesiteinc.htm) |  | 10-Q |  | 3.2 | 11/16/2020 |
| 3.6 | [Certificate of Designation of Series A Preferred Stock dated January 13, 2023](http://www.sec.gov/Archives/edgar/data/1807166/000121390023002835/ea171681ex3-1_amesiteinc.htm) |  | 8-K | 001-39533 | 3.1 | 1/13/2023 |
| 3.7 | [Certificate of Amendment to Certificate of Incorporation](http://www.sec.gov/Archives/edgar/data/1807166/000121390023013326/ea173916ex3-1_amesite.htm) |  | 8-K | 001-39533 | 3.1 | 2/21/2023 |
| 4.1 | [Form of Warrant](http://www.sec.gov/Archives/edgar/data/1807166/000121390022053249/ea165262ex4-1_amesiteinc.htm) |  | 8-K | 001-39533 | 4.1 | 9/1/2022 |
| 4.2 | [Form of Placement Agent Warrant](http://www.sec.gov/Archives/edgar/data/1807166/000121390022053249/ea165262ex4-2_amesiteinc.htm) |  | 8-K | 001-39533 | 4.2 | 9/1/2022 |
| 5.1 | [Opinion of Sheppard, Mullin, Richter & Hampton LLP](ea174829ex5-1_amesiteinc.htm) | X |  |  |  |  |
| 10.1 | [Form of Subscription Agreement.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-1_amesite.htm) |  | S-1 | 333-248801 | 10.1 | 9/4/2020 |
| 10.2 | [Form of Registration Rights Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-2_amesite.htm) |  | S-1 | 333-248801 | 10.2 | 9/4/2020 |
| 10.3 | [Form of Amended and Restated Registration Rights Agreement, dated February 14, 2020.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-3_amesite.htm) |  | S-1 | 333-248801 | 10.3 | 9/4/2020 |
| 10.4 | [Form of Amended and Restated Registration Rights Agreement, dated April 14, 2020.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-4_amesite.htm) |  | S-1 | 333-248801 | 10.4 | 9/4/2020 |
| 10.5 | [Form of Purchase Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-5_amesite.htm) |  | S-1 | 333-248801 | 10.5 | 9/4/2020 |
| 10.6 | [Form of Unsecured Convertible Promissory Note](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-6_amesite.htm) |  | S-1 | 333-248801 | 10.6 | 9/4/2020 |

---

---

| | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|
| | | | **Incorporated by Reference** | **Incorporated by Reference** | **Incorporated by Reference** | **Incorporated by Reference** |
| **Exhibit**<br>**Number** | <br>**Exhibit Title** | **Filed<br> with this**<br>**Form S-1** | **Form** | **File No.** | **Exhibit** | **Date Filed** |
| 10.7+ | [2017 Equity Incentive Plan and forms of award agreements thereunder, assumed in the Reorganization](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-7_amesite.htm) |  | S-1 | 333-248801 | 10.7 | 9/4/2020 |
| 10.8+ | [2018 Equity Incentive Plan and forms of award agreements thereunder, assumed in the Reorganization.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-8_amesite.htm) |  | S-1 | 333-248801 | 10.8 | 9/4/2020 |
| 10.9+ | [Employment Agreement dated as of November 14, 2017 by and between Amesite Operating Company and Ann Marie Sastry, Ph.D.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-9_amesite.htm) |  | S-1 | 333-248801 | 10.9 | 9/4/2020 |
| 10.10 | [Lease Agreement dated as of November 13, 2017 by and between Amesite Operating Company and 205-207 East Washington, LLC.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-10_amesite.htm) |  | S-1 | 333-248801 | 10.10 | 9/4/2020 |
| 10.11+ | [Employment Agreement dated as of April 27, 2018 by and between the Company and Ann Marie Sastry.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-11_amesite.htm) |  | S-1 | 333-248801 | 10.11 | 9/4/2020 |
| 10.12+ | [Executive Agreement, effective as of June 1, 2020, by and between the Company and Ann Marie Sastry.](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-12_amesite.htm) |  | S-1 | 333-248801 | 10.12 | 9/4/2020 |
| 10.13 | [Form of Lock-up Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-13_amesite.htm) |  | S-1 | 333-248801 | 10.13 | 9/4/2020 |
| 10.14 | [Consulting Agreement by between the Company and Richard DiBartolomeo](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-14_amesite.htm) |  | S-1 | 333-248801 | 10.14 | 9/4/2020 |
| 10.15+ | [Employment Offer Letter, dated July 14, 2020, by and between the Company and Richard DiBartolomeo](http://www.sec.gov/Archives/edgar/data/1807166/000121390020025469/ea125860ex10-15_amesite.htm) |  | S-1 | 333-248801 | 10.15 | 9/4/2020 |
| 10.16+ | [Kern Employment Letter, Dated January 31, 2021](http://www.sec.gov/Archives/edgar/data/1807166/000121390021006770/ea134614ex10-1_amesite.htm) |  | 8-K | 001-39533 | 10.1 | 2/4/2021 |
| 10.17 | [Purchase Agreement, dated as of August 2, 2021, between Amesite, Inc. and Lincoln Park Capital Fund, LLC](http://www.sec.gov/Archives/edgar/data/1807166/000121390021040759/ea145360ex10-1_amesiteinc.htm) |  | 8-K | 001-39533 | 10.1 | 8/6/2021 |
| 10.18 | [Registration Rights Agreement, dated as of August 2, 2021, between Amesite, Inc. and Lincoln Park Capital Fund, LLC](http://www.sec.gov/Archives/edgar/data/1807166/000121390021040759/ea145360ex10-2_amesiteinc.htm) |  | 8-K | 001-39533 | 10.2 | 8/6/2021 |
| 10.19 | [Form of Senior Indenture](http://www.sec.gov/Archives/edgar/data/0001807166/000121390021055838/ea149668ex4-2_amesiteinc.htm) |  | S-3 | 333-260666 | 4.2 | 11/1/2021 |
| 10.20 | [Form of Subordinated Indenture](http://www.sec.gov/Archives/edgar/data/0001807166/000121390021055838/ea149668ex4-3_amesiteinc.htm) |  | S-3 | 333-260666 | 4.3 | 11/1/2021 |
| 10.21 | [Corrao Employment Agreement, dated as of December 15, 2021](http://www.sec.gov/Archives/edgar/data/0001807166/000121390021066698/ea152831ex10-1_amesite.htm) |  | 8-K | 001-39533 | 10.1 | 12/21/2021 |
| 10.22 | [Amended and Restated Underwriting Agreement, dated February 12, 2022, by and between the Company and Laidlaw & Company (UK) Ltd., as representative of the several underwriters listed in Schedule I thereto.](http://www.sec.gov/Archives/edgar/data/0001807166/000121390022008107/ea155834ex1-1_amesiteinc.htm) |  | 8-K | 001-39533 | 1.1 | 2/16/2022 |
| 10.23 | [Form of Underwriter's Warrant](http://www.sec.gov/Archives/edgar/data/0001807166/000121390022008107/ea155834ex4-1_amesiteinc.htm) |  | 8-K | 001-39533 | 4.1 | 2/16/2022 |
| 10.24 | [Master Services Agreement, dated August 26, 2022, by and between the Company and NAFEO](http://www.sec.gov/Archives/edgar/data/1807166/000121390022051598/ea165069ex1-1_amesite.htm) |  | 8-K | 001-39533 | 1.1 | 8/29/2022 |
| 10.25 | [Form of Securities Purchase Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390022053249/ea165262ex10-1_amesiteinc.htm) |  | 8-K | 001-39533 | 10.1 | 9/1/2022 |
| 10.26 | [Form of Placement Agency Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390022053249/ea165262ex10-2_amesiteinc.htm) |  | 8-K | 001-39533 | 10.2 | 9/1/2022 |
| 10.27 | [Form of Lock-Up Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390022053249/ea165262ex10-3_amesiteinc.htm) |  | 8-K | 001-39533 | 10.3 | 9/1/2022 |
| 10.28 | [Farrell CFO Agreement](http://www.sec.gov/Archives/edgar/data/1807166/000121390022080131/ea170290ex10-1_amesite.htm) |  | 8-K | 001-39533 | 10.1 | 12/15/2022 |
| 10.29 | [Subscription and Investment Representation Agreement dated January 13, 2023](http://www.sec.gov/Archives/edgar/data/1807166/000121390023002835/ea171681ex10-1_amesiteinc.htm) |  | 8-K | 001-39533 | 10.1 | 1/13/2023 |
| 10.30 | [First Amendment to Amesite Inc. 2018 Equity Incentive Plan](http://www.sec.gov/Archives/edgar/data/1807166/000121390023013326/ea173916ex10-1_amesite.htm) |  | 8-K | 001-39533 | 10.1 | 2/21/2023 |
| 16.1 | [Letter from Deloitte & Touche LLP dated February 2, 2023](http://www.sec.gov/Archives/edgar/data/1807166/000121390023007353/ea172576ex16-1_amesite.htm) |  | 8-K | 001-39533 | 16.1 | 2/2/2023 |
| 23.1 | [Consent of Deloitte & Touche LLP](ea174829ex23-1_amesiteinc.htm) | X |  |  |  |  |
| 23.2 | [Consent of Sheppard, Mullin, Richter & Hampton LLP (included in Exhibit 5.1)](ea174829ex5-1_amesiteinc.htm) | X |  |  |  |  |
| 24.1 | [Power of Attorney (included on signature page)](#a_015) | X |  |  |  |  |
| 107 | [Filing Fee Table](ea174829ex-fee_amesiteinc.htm) | X |  |  |  |  |

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\* Pursuant to Item 601(b)(2) of Regulation S-K promulgated by the SEC, certain schedules have been omitted. The registrant hereby agrees to furnish supplementally to the SEC, upon its request, any or all omitted schedules.

+ Management contracts or compensation plans or arrangements in which directors or executive officers are eligible to participate.

**Item 17. Undertakings**

We hereby undertake:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(1) To file, during any period in which offers or sales are being made, a post-effective amendment to this registration statement:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(i) To include any prospectus required by section 10(a)(3) of the Securities Act of 1933 (the "Securities Act");

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(ii) To reflect in the prospectus any facts or events arising after the effective date of the registration statement (or the most recent post-effective amendment thereof) which, individually or in the aggregate, represent a fundamental change in the information set forth in the registration statement. Notwithstanding the foregoing, any increase or decrease in volume of securities offered (if the total dollar value of securities offered would not exceed that which was registered) and any deviation from the low or high end of the estimated maximum offering range may be reflected in the form of prospectus filed with the SEC pursuant to Rule 424(b) if, in the aggregate, the changes in volume and price represent no more than 20% change in the maximum aggregate offering price set forth in the "Calculation of Registration Fee" table in the effective registration statement; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(iii) To include any material information with respect to the plan of distribution not previously disclosed in the registration statement or any material change to such information in the registration statement;

*provided, however,* that paragraphs (a)(1)(i), (a)(1)(ii) and (a)(1)(iii) of this section do not apply if the information required to be included in a post-effective amendment by those paragraphs is contained in reports filed with or furnished to the SEC by the registrant pursuant to Section 13 or Section 15(d) of the Exchange Act that are incorporated by reference in the registration statement.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(2) That, for the purpose of determining any liability under the Securities Act, each such post-effective amendment shall be deemed to be a new registration statement relating to the securities offered therein, and the offering of such securities at that time shall be deemed to be the initial bona fide offering thereof.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(3) To remove from registration by means of a post-effective amendment any of the securities being registered which remain unsold at the termination of the offering.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(4) That, for the purpose of determining liability under the Securities Act to any purchaser,

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(i) Each prospectus filed by the Registrant pursuant to Rule 424(b)(3) shall be deemed to be part of the registration statement as of the date the filed prospectus was deemed part of and included in the registration statement; and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(ii) Each prospectus required to be filed pursuant to Rule 424(b)(2), (b)(5), or (b)(7) as part of a registration statement in reliance on Rule 430B relating to an offering made pursuant to Rule 415(a)(1)(i), (vii) or (x) for the purpose of providing the information required by Section 10(a) of the Securities Act shall be deemed to be part of and included in the registration statement as of the earlier of the date such form of prospectus is first used after effectiveness or the date of the first contract of sale of securities in the offering described in the prospectus. As provided in Rule 430B, for liability purposes of the issuer and any person that is at that date an underwriter, such date shall be deemed to be a new effective date of the registration statement relating to the securities in the registration statement to which that prospectus relates, and the offering of such securities at that time shall be deemed to be the initial bona fide offering thereof; provided, however, that no statement made in a registration statement or prospectus that is part of the registration statement or made in a document incorporated or deemed incorporated by reference into the registration statement or prospectus that is part of the registration statement will, as to a purchaser with a time of contract of sale prior to such effective date, supersede or modify any statement that was made in the registration statement or prospectus that was part of the registration statement or made in any such document immediately prior to such effective date.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(5) That, for purposes of determining any liability under the Securities Act, each filing of the registrant's annual report pursuant to Section 13(a) or Section 15(d) of the Exchange Act that is incorporated by reference in the registration statement shall be deemed to be a new registration statement relating to the securities offered therein, and the offering of such securities at that time shall be deemed to be the initial bona fide offering thereof.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(6) Insofar as indemnification for liabilities arising under the Securities Act may be permitted to directors, officers, and controlling persons of the registrant pursuant to the foregoing provisions, or otherwise, the registrant has been advised that, in the opinion of the SEC, such indemnification is against public policy as expressed in the Securities Act and is, therefore, unenforceable. In the event that a claim for indemnification against such liabilities (other than the payment by the registrant of expenses incurred or paid by a director, officer or controlling person of the registrant in the successful defense of any action, suit or proceeding) is asserted by such director, officer, or controlling person in connection with the securities being registered, the registrant will, unless in the opinion of its counsel the matter has been settled by controlling precedent, submit to a court of appropriate jurisdiction the question whether such indemnification by it is against public policy as expressed in the Securities Act and will be governed by the final adjudication of such issue.

**SIGNATURES**

Pursuant to the requirements of the Securities Act of 1933, the registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-1 and has duly caused this registration statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Detroit, State of Michigan, on March 14, 2023.

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| | |
|:---|:---|
| **AMESITE INC.** | **AMESITE INC.** |
| By: | /s/ Ann Marie Sastry |
|  | Ann Marie Sastry, Ph.D. |
|  | President and Chief Executive Officer |

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**SIGNATURES AND POWER OF ATTORNEY**

We, the undersigned directors and officers of Amesite Inc., hereby severally constitute and appoint Ann Marie Sastry, his or her true and lawful attorney-in-fact and agent with full power of substitution and re-substitution, for him and in his name, place and stead, in any and all capacities to sign any or all amendments (including, without limitation, post-effective amendments) to this Registration Statement, any related Registration Statement filed pursuant to Rule 462(b) under the Securities Act of 1933, as amended, and any or all pre- or post-effective amendments thereto, and to file the same, with all exhibits thereto, and all other documents in connection therewith, with the Securities and Exchange Commission, granting unto said attorney-in-fact and agent, full power and authority to do and perform each and every act and thing requisite and necessary to be done in and about the premises, as fully for all intents and purposes as he or she might or could do in person, hereby ratifying and confirming that said attorney-in-fact and agent, or any substitute or substitutes for him, may lawfully do or cause to be done by virtue hereof.

Pursuant to the requirements of the Securities Act of 1933, as amended, this registration statement on Form S-1 has been signed by the following persons in the capacities and on the dates indicated.

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| | | |
|:---|:---|:---|
| **Signature** | **Title** | **Date** |
| /s/ Ann Marie Sastry, Ph.D. | Chief Executive Officer, President and Chairman of the Board | March 14, 2023 |
| Ann Marie Sastry, Ph.D. | (*Principal Executive Officer)* |  |
| /s/ Sherlyn W. Farrell | Chief Financial Officer | March 14, 2023 |
| Sherlyn W. Farrell | *(Principal Financial Officer and Principal Accounting Officer)* |  |
| /s/ Anthony M. Barkett | Director | March 14, 2023 |
| Anthony M. Barkett |  |  |
| /s/ Barbie Brewer | Director | March 14, 2023 |
| Barbie Brewer |  |  |
| /s/ Michael Losh | Director | March 14, 2023 |
| Michael Losh |  |  |
| /s/ Richard T. Ogawa | Director | March 14, 2023 |
| Richard T. Ogawa |  |  |
| /s/ Gilbert S. Omenn, M.D., Ph.D. | Director | March 14, 2023 |
| Gilbert S. Omenn, M.D., Ph.D. |  |  |
| /s/ George Parmer | Director | March 14, 2023 |
| George Parmer |  |  |

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## Exhibit 5.1

**Exhibit 5.1**

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| | |
|:---|:---|
| ![](image_001.jpg) | Sheppard, Mullin, Richter & Hampton LLP<br> 30 Rockefeller Plaza<br> New York, New York 10112-0015<br> 212.653.8700 main<br> 212.653.8701 fax<br> www.sheppardmullin.com |

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March 14, 2023

**VIA EDGAR**

Amesite Inc.

607 Shelby Street

Suite 700 PMB 214

Detroit, MI 48226

Re: <u>Registration Statement on Form S-1</u>

Ladies and Gentlemen:

We have acted as counsel to Amesite Inc., a Delaware corporation (the "<u>Company</u>"), in connection with the issuance of this opinion that relates to a Registration Statement on Form S-1 (the "<u>Registration Statement</u>") filed by the Company with the United States Securities and Exchange Commission (the "<u>SEC</u>") under the Securities Act of 1933, as amended (the "<u>Securities Act</u>"). The Registration Statement covers the resale, by the selling stockholders listed therein, from time to time pursuant to Rule 415 under the Securities Act as set forth in the Registration Statement, of 366,665 shares of the Company's common stock, par value $0.001 per share (the "<u>Common Stock</u>"), which consist of (i) 349,240 shares of Common Stock that may be issuable upon exercise of certain outstanding warrants (the "<u>Warrants</u>"), and (ii) 17,425 shares of Common Stock that may be issuable upon exercise of certain outstanding placement agent warrants (the "<u>Placement Agent Warrants</u>"). The shares of Common Stock issuable upon exercise of the Warrants and Placement Agent Warrants are referred to herein as the "Shares."

This opinion letter is being delivered in accordance with the requirements of Item 601(b)(5)(i) of Regulation S-K under the Securities Act, and no opinion is expressed herein as to any matter pertaining to the contents of the Registration Statement.

In connection with the issuance of this opinion letter, we have examined originals or copies, certified or otherwise identified to our satisfaction, of such records of the Company and such agreements, certificates and receipts of public officials, certificates of officers or other representatives of the Company and others, and such other documents as we have deemed necessary or appropriate as a basis for the opinions stated below. As to any facts relevant to the opinions stated herein that we did not independently establish or verify, we have relied upon statements and representations of officers and other representatives of the Company and of public officials.

In our examination, we have assumed (a) the genuineness of all signatures, including endorsements, (b) the legal capacity and competency of all natural persons, (c) the authenticity of all documents submitted to us as originals, (d) the conformity to original documents of all documents submitted to us as facsimile, electronic, certified or photostatic copies, and the authenticity of the originals of such copies; and (e) the accuracy, completeness and authenticity of certificates of public officials.

Based upon the foregoing and subject to the qualifications and assumptions stated herein, we are of the opinion that the Shares have been duly authorized by all requisite corporate action on the part of the Company under the Delaware General Corporation Law (the "<u>DGCL</u>") and, when issued in accordance with the terms and conditions of the Warrants and the Placement Agent Warrants, as applicable, will be validly issued, fully paid, and non-assessable.

Our opinion is expressly limited to the matters set forth above, and we render no opinion, whether by implication or otherwise, as to any other matters relating to the Company, the Warrants, the Placement Agent Warrants the Shares or any other agreements or transactions that may be related thereto or contemplated thereby. No opinion may be implied or inferred beyond the opinion expressly stated above.

The opinion we render herein is limited to those matters governed by the DGCL as of the date hereof and we disclaim any obligation to revise or supplement the opinion rendered herein should the above-referenced laws be changed by legislative or regulatory action, judicial decision, or otherwise. We express no opinion as to whether, or the extent to which, the laws of any particular jurisdiction apply to the subject matter hereof.

This opinion letter is rendered as of the date first written above, and we disclaim any obligation to advise you of facts, circumstances, events, or developments that hereafter may be brought to our attention or that may alter, affect, or modify the opinion expressed herein.

We hereby consent to the filing of this opinion as an exhibit to the Registration Statement. We also hereby consent to the reference to our firm under the heading "Legal Matters" in the Registration Statement. In giving this consent, we do not thereby admit that we are within the category of persons whose consent is required under Section 7 of the Securities Act or the General Rules and Regulations under the Securities Act. It is understood that this opinion is to be used only in connection with the offer and sale of the Initial Purchase Shares, the Commitment Shares and the Purchase Shares being registered while the Registration Statement is effective under the Securities Act.

Respectfully submitted,

*<u>/s/ Sheppard, Mullin, Richter & Hampton LLP</u>*

Sheppard, Mullin, Richter & Hampton LLP

## Exhibit 23.1

**Exhibit 23.1**

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the incorporation by reference in this Registration Statement on Form S-1 of our report dated September 28, 2022, relating to the financial statements of Amesite, Inc., appearing in the Annual Report on Form 10-K of Amesite, Inc. for the year ended June 30, 2022.

We also consent to the reference to us under the heading "Experts" in such Registration Statement.

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| |
|:---|
| /s/ Deloitte & Touche LLP |
| Detroit, MI |
| March 13, 2023 |

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## Ex-Filing

**Exhibit 107**

**Calculation of Filing Fee Tables**

**Form S-1**

(Form Type)

**Amesite Inc.**

(Exact Name of Registrant as Specified in its Charter)

**Table 1: Newly Registered and Carry Forward Securities**

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| | | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|:---|
|  | **Security Type** | **Security<br> Class<br> Title** | **Fee<br> Calculation<br> or Carry<br> Forward <br> Rule** | **Amount<br> Registered(1)** | **Proposed<br> Maximum<br> Offering Price<br> Per Unit(2)** | **Maximum<br> Aggregate<br> Offering Price(2)** | **Fee Rate** | **Amount of<br> Registration Fee** |
| **Fees to Be Paid** | Equity | Common Stock, $0.0001 par value(3) | 457(c) | 366665 | $2.82 | $1035278.63 | $0.00011020 | $114.09 |
| **Fees Previously Paid** |  |  |  |  |  |  |  | $0 |
|  | **Total Offering Amounts** |  |  |  |  |  |  | $114.09 |
|  | **Total Fees Previously Paid** |  |  |  |  |  |  |  |
|  | **Total Fee Offsets** |  |  |  |  |  |  |  |
|  |  | **Net Fee Due** |  |  |  |  |  | $114.09 |

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&nbsp;&nbsp;&nbsp;&nbsp;(1) Pursuant to Rule 416 under the Securities Act of 1933 (the "Securities Act"), the securities being registered hereunder also include such indeterminate number of additional shares of common stock as may from time to time be issued after the date hereof as a result of stock splits, stock dividends, recapitalizations or similar transactions.

&nbsp;&nbsp;&nbsp;&nbsp;(2) Estimated solely for the purpose of calculating the registration fee in accordance with Rule 457(c) of the Securities Act, based on the average of the high and low prices of the common stock of the registrant as reported on The Nasdaq Capital Market on March 10, 2023.

&nbsp;&nbsp;&nbsp;&nbsp;(3) Represents shares of our common stock offered by the selling stockholders issuable upon the exercise of warrants to purchase common stock issued to the selling stockholders in a private placement.