# EDGAR Filing Document

**Accession Number:** 0000906185
**File Stem:** 0001145549-25-057311
**Filing Date:** 2025-8
**Character Count:** 31545
**Document Hash:** 677812ca68de6f99526239f008c21cf0
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001145549-25-057311.hdr.sgml**: 20250829

**ACCESSION NUMBER**: 0001145549-25-057311

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250630

**FILED AS OF DATE**: 20250829

**DATE AS OF CHANGE**: 20250829

**PERIOD START**: 20251231

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS ASPEN SERIES
- **CENTRAL INDEX KEY:** 0000906185

**ORGANIZATION NAME:**
- **EIN:** 841235540
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-07736
- **FILM NUMBER:** 251279171

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 3033333863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

## Series and Classes Contracts Data

### Janus Henderson Research Portfolio (Series ID: S000010395)

| Class ID   | Class Name           | Ticker Symbol   |
|:---|:---|:---|
| C000028718 | Service Shares       |  |
| C000028719 | Institutional Shares | JAGRX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS ASPEN SERIES

- **b. Investment Company Act file number:** 811-07736

- **c. CIK number of Registrant:** 0000906185

- **d. LEI of Registrant:** 5C1H794C69TI10A0XC04

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 DETROIT STREET

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Research Portfolio

- **b. EDGAR series identifier (if any):** S000010395

- **c. LEI of Series:** NWP85B4YG8FG3WDYMV29

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-12-31

- **b. Date as of which information is reported:** 2025-06-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $749143852.67

**Total Liabilities:** $692683.25

**Net Assets:** $748451169.42

**Cash Not Reported:** $84824.85

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028718 | 2.20%                | 9.82%                | 7.26%                |
| Class ID C000028719 | 2.23%                | 9.83%                | 7.28%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $2706611.00              | $11257237.00                               |
| Month 2  | $8933556.00              | $54462704.00                               |
| Month 3  | $27670328.00             | $23521879.00                               |

**Designated Index Information**

- **Index Name:** Russell 1000 Growth Total Return Index

- **Index Identifier:** RUSSELL.MC.RLG

### Schedule of Portfolio Investments

| Name                                 | Title                                           | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Alphabet Inc.                        | Alphabet Inc. CAP USD0.001 CL C                 | CUSIP: 02079K107<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |    137564 | NS      | $24402477.96  | 3.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Amazon.com, Inc.                     | Amazon.com, Inc. COM USD0.01                    | CUSIP: 023135106<br>LEI: ZXTILKJKG63JELOEG630 | Long             | EC               | CORP              | US        |    186951 | NS      | $41015179.89  | 5.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                           | Amgen Inc. COM USD0.0001                        | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |     11689 | NS      | $3263685.69   | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| Amphenol Corporation                 | Amphenol Corporation CL'A'COM USD0.001          | CUSIP: 032095101<br>LEI: 549300D3L3G0R4U4VT04 | Long             | EC               | CORP              | US        |     53382 | NS      | $5271472.50   | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Analog Devices Inc.                  | Analog Devices Inc. COM USD0.16 2/3             | CUSIP: 032654105<br>LEI: GYVOE5EZ4GDAVTU4CQ61 | Long             | EC               | CORP              | US        |     12895 | NS      | $3069267.90   | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Apollo Global Management Inc         | Apollo Global Management Inc COM                | CUSIP: 03769M106<br>LEI: 254900LMFT1CY9IYB476 | Long             | EC               | CORP              | US        |     20226 | NS      | $2869462.62   | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| DexCom Inc                           | DexCom Inc COM USD0.001                         | CUSIP: 252131107<br>LEI: 549300YSK3QDSFR5EU59 | Long             | EC               | CORP              | US        |     28309 | NS      | $2471092.61   | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                         | DoorDash Inc CL A                               | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |     19195 | NS      | $4731759.45   | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Dynatrace Inc                        | Dynatrace Inc COM USD0.001                      | CUSIP: 268150109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     45771 | NS      | $2527016.91   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Ecolab Inc.                          | Ecolab Inc. COM USD1                            | CUSIP: 278865100<br>LEI: 82DYEISM090VG8LTLS26 | Long             | EC               | CORP              | US        |      7089 | NS      | $1910060.16   | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson Enterprises Inc             | Ferguson Enterprises Inc COMMON STOCK NEW       | CUSIP: 31488V107<br>LEI: 2138003JYQMRP3SLX189 | Long             | EC               | CORP              | US        |     16733 | NS      | $3643610.75   | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| GE Aerospace                         | GE Aerospace COM NEW                            | CUSIP: 369604301<br>LEI: 3C7474T6CDKPR9K6YT90 | Long             | EC               | CORP              | US        |     37774 | NS      | $9722649.86   | 1.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Hilton Worldwide Holdings Inc        | Hilton Worldwide Holdings Inc COM USD0.01       | CUSIP: 43300A203<br>LEI: 549300HVGPK36ICB0B89 | Long             | EC               | CORP              | US        |     15027 | NS      | $4002291.18   | 0.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Howmet Aerospace Inc                 | Howmet Aerospace Inc COM                        | CUSIP: 443201108<br>LEI: 549300HO5WFZUT5N2T22 | Long             | EC               | CORP              | US        |     41458 | NS      | $7716577.54   | 1.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Hubspot Inc                          | Hubspot Inc COM USD0.001                        | CUSIP: 443573100<br>LEI: 529900BLNVEW0MVKJB37 | Long             | EC               | CORP              | US        |      5572 | NS      | $3101542.36   | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuit Inc                           | Intuit Inc COM USD0.01                          | CUSIP: 461202103<br>LEI: VI90HBPH7XSFMB9E4M29 | Long             | EC               | CORP              | US        |     14522 | NS      | $11437962.86  | 1.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.              | Intuitive Surgical Inc. COM USD0.001            | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |      9950 | NS      | $5406929.50   | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                            | KLA Corp. COM USD0.001                          | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |      5628 | NS      | $5041224.72   | 0.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Lam Research Corporation             | Lam Research Corporation COM NEW                | CUSIP: 512807306<br>LEI: 549300I4GMO6D34U1T02 | Long             | EC               | CORP              | US        |     51181 | NS      | $4981958.54   | 0.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                      | Eli Lilly & Co. COM NPV                         | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |     23931 | NS      | $18654932.43  | 2.49%             |  |  |  | No            |                  1 | On Loan: No      |
| 3M Company                           | 3M Company COM USD0.01                          | CUSIP: 88579Y101<br>LEI: LUZQVYP4VS22CLWDAR65 | Long             | EC               | CORP              | US        |     46889 | NS      | $7138381.36   | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC              | Janus Henderson Cash Liquidity Fund LLC         | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |   2587015 | NS      | $2587532.19   | 0.35%             |  |  |  | No            |                  2 | On Loan: No      |
| Eaton Corporation PLC                | Eaton Corporation PLC COM USD0.01               | CUSIP: G29183103<br>LEI: 549300VDIGTMXUNT7H71 | Long             | EC               | CORP              | US        |     13446 | NS      | $4800087.54   | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Flutter Entertainment PLC            | Flutter Entertainment PLC ORD EUR0.09 (DI)      | CUSIP: G3643J108<br>LEI: 635400EG4YIJLJMZJ782 | Long             | EC               | CORP              | US        |     12346 | NS      | $3527992.96   | 0.47%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                           | Apple Inc. COM USD0.00001                       | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |    230420 | NS      | $47275271.40  | 6.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Applovin Corp.                       | Applovin Corp. COM CL A                         | CUSIP: 03831W108<br>LEI: 549300LLVXMUAOL3SQ07 | Long             | EC               | CORP              | US        |     11852 | NS      | $4149148.16   | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Ares Management Corporation          | Ares Management Corporation COM USD0.01 CLASS A | CUSIP: 03990B101<br>LEI: 529900JUV61FH7B8VW38 | Long             | EC               | CORP              | US        |     18399 | NS      | $3186706.80   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                            | Argenx SE SPON ADR EACH REP 1 ORD SHS           | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |      3587 | NS      | $1977226.14   | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                      | AstraZeneca PLC SPON ADR EACH REP 0.50 ORD      | CUSIP: 046353108<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             |  | CORP              | US        |     40435 | NS      | $2825597.80   | 0.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlassian Corporation                | Atlassian Corporation CL A                      | CUSIP: 049468101<br>LEI: 549300V7ZY5P02D2MY38 | Long             | EC               | CORP              | US        |     13449 | NS      | $2731357.41   | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Autodesk Inc                         | Autodesk Inc COM USD0.01                        | CUSIP: 052769106<br>LEI: FRKKVKAIQEF3FCSTPG55 | Long             | EC               | CORP              | US        |     23898 | NS      | $7398103.86   | 0.99%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.             | Avidity Biosciences Inc. COM                    | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20883 | NS      | $593077.20    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Blackstone Inc                       | Blackstone Inc COM USD0.00001 CLASS A           | CUSIP: 09260D107<br>LEI: 5299004LW4QWGZUB8Y96 | Long             | EC               | CORP              | US        |     32341 | NS      | $4837566.78   | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| The Boeing Company                   | The Boeing Company COM USD5.00                  | CUSIP: 097023105<br>LEI: RVHJWBXLJ1RFUBSY1F30 | Long             | EC               | CORP              | US        |     16480 | NS      | $3453054.40   | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc                 | Booking Holdings Inc COM USD0.008               | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |      1510 | NS      | $8741752.40   | 1.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation        | Boston Scientific Corporation COM USD0.01       | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |     23858 | NS      | $2562587.78   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadcom Inc                         | Broadcom Inc COM USD0.001                       | CUSIP: 11135F101<br>LEI: 549300WV6GIDOZJTV909 | Long             | EC               | CORP              | US        |    141800 | NS      | $39087170.00  | 5.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Cadence Design Systems, Inc.         | Cadence Design Systems, Inc. COM USD0.01        | CUSIP: 127387108<br>LEI: GCT7RXJOGLXPV0NXZY22 | Long             | EC               | CORP              | US        |     19936 | NS      | $6143278.40   | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Capital One Financial Corp.          | Capital One Financial Corp. COM USD0.01         | CUSIP: 14040H105<br>LEI: ZUE8T73ROZOF6FLBAR73 | Long             | EC               | CORP              | US        |     11934 | NS      | $2539077.84   | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Chipotle Mexican Grill Inc           | Chipotle Mexican Grill Inc COM USD0.01          | CUSIP: 169656105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    102983 | NS      | $5782495.45   | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Brands, Inc.           | Constellation Brands, Inc. COM USD0.01 CLASS A  | CUSIP: 21036P108<br>LEI: 5493005GKEG8QCVY7037 | Long             | EC               | CORP              | US        |      7379 | NS      | $1200415.72   | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                  | Danaher Corporation COM USD0.01                 | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |      6690 | NS      | $1321542.60   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Datadog Inc                          | Datadog Inc COM USD0.00001 CL A                 | CUSIP: 23804L103<br>LEI: 549300F6JNO0KRPO1K63 | Long             | EC               | CORP              | US        |     40443 | NS      | $5432708.19   | 0.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Deere & Company                      | Deere & Company COM USD1                        | CUSIP: 244199105<br>LEI: PWFTNG3EI0Y73OXWDH08 | Long             | EC               | CORP              | US        |      8303 | NS      | $4221992.47   | 0.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Gallagher Aj &Co                     | Gallagher Aj &Co COM USD1.00                    | CUSIP: 363576109<br>LEI: 54930049QLLMPART6V29 | Long             | EC               | CORP              | US        |      9092 | NS      | $2910531.04   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Atlas Energy Solutions Inc.          | Atlas Energy Solutions Inc. COM NEW             | CUSIP: 642045108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     69346 | NS      | $927156.02    | 0.12%             |  |  |  | No            |                  1 | On Loan: —       |
| ASML Holding N.V.                    | ASML Holding N.V. NY REGISTRY SHS               | CUSIP: N07059210<br>LEI: 724500Y6DUVHQD6OXN27 | Long             | EC               | CORP              | US        |      2613 | NS      | $2094032.07   | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| The Procter & Gamble Co.             | The Procter & Gamble Co. COM NPV                | CUSIP: 742718109<br>LEI: 2572IBTT8CCZW6AU4141 | Long             | EC               | CORP              | US        |     43967 | NS      | $7004822.44   | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Procore Technologies Inc             | Procore Technologies Inc COM                    | CUSIP: 74275K108<br>LEI: 5493005PTWY0RHYRAH22 | Long             | EC               | CORP              | US        |     26918 | NS      | $1841729.56   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| The Progressive Corporation          | The Progressive Corporation COM USD1.00         | CUSIP: 743315103<br>LEI: 529900TACNVLY9DCR586 | Long             | EC               | CORP              | US        |     10450 | NS      | $2788687.00   | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Servicenow Inc                       | Servicenow Inc COM USD0.001                     | CUSIP: 81762P102<br>LEI: 549300HJTQM36M0E1G39 | Long             | EC               | CORP              | US        |      7136 | NS      | $7336378.88   | 0.98%             |  |  |  | No            |                  1 | On Loan: No      |
| Synopsys Inc                         | Synopsys Inc COM USD0.01                        | CUSIP: 871607107<br>LEI: QG7T915N9S0NY5UKNE63 | Long             | EC               | CORP              | US        |      9336 | NS      | $4786380.48   | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| TFI International Inc                | TFI International Inc COM                       | CUSIP: 87241L109<br>LEI: 5493007R9W7KO7SJN678 | Long             | EC               | CORP              | US        |     26069 | NS      | $2337607.23   | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| The TJX Companies Inc.               | The TJX Companies Inc. COM USD1                 | CUSIP: 872540109<br>LEI: V167QI9I69W364E2DY52 | Long             | EC               | CORP              | US        |     55714 | NS      | $6880121.86   | 0.92%             |  |  |  | No            |                  1 | On Loan: No      |
| T-Mobile US Inc                      | T-Mobile US Inc COM USD0.00001                  | CUSIP: 872590104<br>LEI: 549300QHIJYOHPACPG31 | Long             | EC               | CORP              | US        |     13136 | NS      | $3129783.36   | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Tesla Inc                            | Tesla Inc COM USD0.001                          | CUSIP: 88160R101<br>LEI: 54930043XZGB27CTOV49 | Long             | EC               | CORP              | US        |     11882 | NS      | $3774436.12   | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Tyler Technologies Inc.              | Tyler Technologies Inc. COM USD0.01             | CUSIP: 902252105<br>LEI: 25490040SMU4Z8LBCW70 | Long             | EC               | CORP              | US        |      5375 | NS      | $3186515.00   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Inc.              | UnitedHealth Group Inc. COM USD0.01             | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |      3650 | NS      | $1138690.50   | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                          | Vaxcyte Inc COM                                 | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |     13274 | NS      | $431537.74    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Meta Platforms Inc                   | Meta Platforms Inc COM USD0.000006 CL 'A'       | CUSIP: 30303M102<br>LEI: BQ4BKCS1HXDV9HN80Z93 | Long             | EC               | CORP              | US        |     59494 | NS      | $43911926.46  | 5.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del               | Liberty Media Corp Del COM LBTY ONE S C         | CUSIP: 531229755<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |     74602 | NS      | $7795909.00   | 1.04%             |  |  |  | No            |                  1 | On Loan: No      |
| MasterCard Incorporated              | MasterCard Incorporated COM USD0.0001 CLASS A   | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |     26425 | NS      | $14849264.50  | 1.98%             |  |  |  | No            |                  1 | On Loan: No      |
| Verona Pharma Plc                    | Verona Pharma Plc SPONSORED ADS                 | CUSIP: 925050106<br>LEI: 213800EVI6O6J3TIAL06 | Long             |  | CORP              | US        |     15964 | NS      | $1509875.12   | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Visa Inc                             | Visa Inc COM STK USD0.0001                      | CUSIP: 92826C839<br>LEI: 549300JZ4OKEHW3DPJ59 | Long             | EC               | CORP              | US        |     49782 | NS      | $17675099.10  | 2.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Mckesson Corp.                       | Mckesson Corp. COM USD0.01                      | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |      4911 | NS      | $3598682.58   | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Trane Technologies PLC               | Trane Technologies PLC COM USD1                 | CUSIP: G8994E103<br>LEI: 549300BURLR9SLYY2705 | Long             | EC               | CORP              | US        |     13220 | NS      | $5782560.20   | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| Spotify Technology S.A.              | Spotify Technology S.A. COM EUR0.000625         | CUSIP: L8681T102<br>LEI: 549300B4X0JHWV0DTD60 | Long             | EC               | CORP              | US        |      8756 | NS      | $6718829.04   | 0.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated  | Vertex Pharmaceuticals Incorporated COM USD0.01 | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |     10218 | NS      | $4549053.60   | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Vistra Corp                          | Vistra Corp COM USD0.01                         | CUSIP: 92840M102<br>LEI: 549300KP43CPCUJOOG15 | Long             | EC               | CORP              | US        |     20580 | NS      | $3988609.80   | 0.53%             |  |  |  | No            |                  1 | On Loan: No      |
| LPL Financial Holdings Inc.          | LPL Financial Holdings Inc. COM USD0.001        | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |      8652 | NS      | $3244240.44   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc                | Lantheus Holdings Inc COM USD0.01               | CUSIP: 516544103<br>LEI: 5299005HUB5IOJGO7S39 | Long             | EC               | CORP              | US        |     15426 | NS      | $1262772.36   | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Wayfair Inc                          | Wayfair Inc COM USD0.001 A                      | CUSIP: 94419L101<br>LEI: 54930027N9ZAVYFDHK53 | Long             | EC               | CORP              | US        |     27520 | NS      | $1407372.80   | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Wingstop Inc                         | Wingstop Inc COM USD0.01                        | CUSIP: 974155103<br>LEI: 549300SSH3F8HPIM3Q47 | Long             | EC               | CORP              | US        |      2549 | NS      | $858350.26    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corp.                      | Microsoft Corp. COM USD0.00000625               | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |    202117 | NS      | $100535016.97 | 13.43%            |  |  |  | No            |                  1 | On Loan: No      |
| Monster Beverage Corp                | Monster Beverage Corp COM USD0.005              | CUSIP: 61174X109<br>LEI: 52990090AP0E7HCB6F33 | Long             | EC               | CORP              | US        |     33304 | NS      | $2086162.56   | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Netflix, Inc.                        | Netflix, Inc. COM USD0.001                      | CUSIP: 64110L106<br>LEI: 549300Y7VHGU0I7CE873 | Long             | EC               | CORP              | US        |     13392 | NS      | $17933628.96  | 2.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Nike, Inc.                           | Nike, Inc. CLASS'B'COM NPV                      | CUSIP: 654106103<br>LEI: 787RXPR0UX0O0XUXPZ81 | Long             | EC               | CORP              | US        |     33830 | NS      | $2403283.20   | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| NVIDIA Corp.                         | NVIDIA Corp. COM USD0.001                       | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |    659748 | NS      | $104233586.52 | 13.93%            |  |  |  | No            |                  1 | On Loan: No      |
| O'Reilly Automotive Inc.             | O'Reilly Automotive Inc. USD0.01                | CUSIP: 67103H107<br>LEI: 549300K2RLMQL149Q332 | Long             | EC               | CORP              | US        |     55054 | NS      | $4962017.02   | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| ON Semiconductor Corp.               | ON Semiconductor Corp. COM USD0.01              | CUSIP: 682189105<br>LEI: ZV20P4CNJVT8V1ZGJ064 | Long             | EC               | CORP              | US        |     56015 | NS      | $2935746.15   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Oracle Corp.                         | Oracle Corp. COM USD0.01                        | CUSIP: 68389X105<br>LEI: 1Z4GXXU7ZHVWFCD8TV52 | Long             | EC               | CORP              | US        |     33811 | NS      | $7392098.93   | 0.99%             |  |  |  | No            |                  1 | On Loan: No      |
| Janus Henderson Cash Collateral Fund | Janus Henderson Cash Collateral Fund            | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |    461160 | NS      | $461160.00    | 0.06%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                  | Royal Bank of Canada                            | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             | CORP              | US        |    115290 | PA      | $115290.00    | 0.02%             | 2025-07-01      | Fixed         | 4.31%                 | No            |                  2 | On Loan: No      |

### Signature

**Date Signed:** 2025-08-14

**Name of Applicant:** JANUS ASPEN SERIES

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER