# EDGAR Filing Document

**Accession Number:** 0001270521
**File Stem:** 0001104659-26-060131
**Filing Date:** 2026-5
**Character Count:** 12686
**Document Hash:** 71fbde8396138cdd02417397531be992
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001104659-26-060131.hdr.sgml**: 20260513

**ACCESSION NUMBER**: 0001104659-26-060131

**CONFORMED SUBMISSION TYPE**: 4

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20260511

**FILED AS OF DATE**: 20260513

**DATE AS OF CHANGE**: 20260513

**REPORTING-OWNER**: 

**OWNER DATA:**
- **COMPANY CONFORMED NAME:** KOLLURI KRISHNA KITTU
- **CENTRAL INDEX KEY:** 0001270521

**ORGANIZATION NAME:**

**FILING VALUES:**
- **FORM TYPE:** 4
- **SEC ACT:** 1934 Act
- **SEC FILE NUMBER:** 001-42934
- **FILM NUMBER:** 26972601
**ISSUER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** BillionToOne, Inc.
- **CENTRAL INDEX KEY:** 0002070849
- **STANDARD INDUSTRIAL CLASSIFICATION:** SERVICES-MEDICAL LABORATORIES [8071]
- **ORGANIZATION NAME:** 08 Industrial Applications and Services
- **EIN:** 811082020
- **STATE OF INCORPORATION:** DE
- **FISCAL YEAR END:** 1231

**BUSINESS ADDRESS:**
- **STREET 1:** 1035 O'BRIEN DRIVE
- **CITY:** MENLO PARK
- **STATE:** CA
- **BUSINESS PHONE:** 650-460-2551

**MAIL ADDRESS:**
- **STREET 1:** 1035 O'BRIEN DRIVE
- **CITY:** MENLO PARK
- **STATE:** CA
- **ZIP:** 94025

### UNITED STATES SECURITIES AND EXCHANGE COMMISSION
**Washington, D.C. 20549**

## FORM 4

### STATEMENT OF CHANGES IN BENEFICIAL OWNERSHIP

[ ] Check this box if no longer subject to Section 16. Form 4 or Form 5 obligations may continue. See Instruction 1(b).

[ ] Check this box to indicate that a transaction was made pursuant to a contract, instruction or written plan for the purchase or sale of equity securities of the issuer that is intended to satisfy the affirmative defense conditions of Rule 10b5-1(c). See Instruction 10.

---

| | | |
|:---|:---|:---|
| **1. Name and Address of Reporting Person**<sup>*</sup><br>KOLLURI KRISHNA KITTU<br><sub>(Last) (First) (Middle)</sub><br>1300 EL CAMINO REAL, SUITE 100<br><sub>(Street)</sub><br>MENLO PARK, CA 94025<br><sub>(City) (State) (Zip)</sub> | **3. Date of Earliest Transaction (Month/Day/Year)**<br>2026-05-11 | **5. Relationship of Reporting Person(s) to Issuer**<br>(Check all applicable)<br>[X] Director [X] 10% Owner<br>[ ] Officer (give title below) [ ] Other (specify below)<br>_ _ |
| **2. Issuer Name and Ticker or Trading Symbol**<br>BillionToOne, Inc. [ BLLN ] | **4. If Amendment, Date of Original Filed (Month/Day/Year)**<br>  | **6. Individual or Joint/Group Filing (Check Applicable Line)**<br>[X] Form filed by One Reporting Person<br>[ ] Form filed by More than One Reporting Person |

---

## Table I - Non-Derivative Securities

---

|  |  |  |  |  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| 1. Title of Security | 2. Transaction Date | 2A. Deemed Execution Date | 3. Transaction Code (V) | 3. Transaction Code (V) | 4. Securities Acquired (A) or Disposed of (D) | 4. Securities Acquired (A) or Disposed of (D) | 4. Securities Acquired (A) or Disposed of (D) | 5. Amount of Securities Beneficially Owned | 6. Ownership Form | 7. Nature of Indirect Beneficial Ownership |
| 1. Title of Security | 2. Transaction Date | 2A. Deemed Execution Date | Code | V | Amount | (A) or (D) | Price | 5. Amount of Securities Beneficially Owned | 6. Ownership Form | 7. Nature of Indirect Beneficial Ownership |
| Class A Common Stock | 2026-05-11 |  | J<sup>(1)</sup> |  | 129780 | D | $0 | 1168019 | I | See Footnote<sup>(2)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(3)</sup> |  | 7980 | A | $0 | 7980 | I | See Footnote<sup>(4)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(5)</sup> |  | 7980 | D | $0 | 0 | I | See Footnote<sup>(4)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(6)</sup> |  | 24222 | D | $0 | 217994 | I | See Footnote<sup>(7)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(8)</sup> |  | 1425 | A | $0 | 1425 | I | See Footnote<sup>(9)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(10)</sup> |  | 1425 | D | $0 | 0 | I | See Footnote<sup>(9)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(11)</sup> |  | 271028 | D | $0 | 2439258 | I | See Footnote<sup>(12)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(13)</sup> |  | 5423 | A | $0 | 5423 | I | See Footnote<sup>(14)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(15)</sup> |  | 5423 | D | $0 | 0 | I | See Footnote<sup>(14)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(16)</sup> |  | 10921 | D | $0 | 98283 | I | See Footnote<sup>(17)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(3)</sup> |  | 719 | A | $0 | 719 | I | See Footnote<sup>(18)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(3)</sup> |  | 719 | A | $0 | 719 | I | See Footnote<sup>(19)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(20)</sup> |  | 5761 | A | $0 | 5761 | I | See Footnote<sup>(21)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(22)</sup> |  | 1186 | A | $0 | 6947 | I | See Footnote<sup>(21)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(23)</sup> |  | 2171 | A | $0 | 9118 | I | See Footnote<sup>(21)</sup> |
| Class A Common Stock | 2026-05-11 |  | J<sup>(24)</sup> |  | 5644 | A | $0 | 14762 | I | See Footnote<sup>(21)</sup> |

---

## Table II - Derivative Securities

---

|  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
| 1. Title of Derivative Security | 2. Conversion or Exercise Price | 3. Transaction Date | 3A. Deemed Execution Date | 4. Transaction Code (V) | 4. Transaction Code (V) | 5. Number of Derivative Securities Acquired (A) or Disposed of (D) | 5. Number of Derivative Securities Acquired (A) or Disposed of (D) | 6. Date Exercisable and Expiration Date | 6. Date Exercisable and Expiration Date | 7. Title and Amount of Underlying Securities | 7. Title and Amount of Underlying Securities | 8. Price of Derivative Security | 9. Number of Derivative Securities Beneficially Owned | 10. Ownership Form | 11. Nature of Indirect Beneficial Ownership |
| 1. Title of Derivative Security | 2. Conversion or Exercise Price | 3. Transaction Date | 3A. Deemed Execution Date | Code | V | (A) | (D) | Date Exercisable | Expiration Date | Title | Amount or Number of Shares | 8. Price of Derivative Security | 9. Number of Derivative Securities Beneficially Owned | 10. Ownership Form | 11. Nature of Indirect Beneficial Ownership |
|  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |  |

---

### Footnotes:

(1) Represents a pro-rata, in-kind distribution by Neotribe Ignite Fund I, L.P. ("NTIF I") to its partners, for no additional consideration.

(2) The reported security is directly held by NTIF I. Neotribe Ignite Partners I, LLC ("NTIP I") is the general partner of NTIF I. Krishna Kittu Kolluri ("Kolluri") is the managing member of NTIP I and therefore may be deemed to have voting and dispositive power over the shares held by NTIF I. Kolluri disclaims beneficial ownership of the shares held by NTIF I except to the extent of his pecuniary interest therein.

(3) The shares were obtained pursuant to a pro-rata, in-kind distribution from NTIF I, for no additional consideration.

(4) The reported security is directly held by NTIP I. Kolluri is the managing member of NTIP I and therefore may be deemed to have voting and dispositive power over the shares held by NTIP I. Kolluri disclaims beneficial ownership of the shares held by NTIP I except to the extent of his pecuniary interest therein.

(5) Represents a pro-rata, in-kind distribution by NTIP I to its members and assignees, for no additional consideration.

(6) Represents a pro-rata, in-kind distribution by Neotribe SPV I BTO, LLC ("NT SPV I") to its members, for no additional consideration.

(7) The reported security is directly held by NT SPV I. Neotribe Partners SPV I BTO, LLC ("NTP SPV I") is the managing member of NT SPV I. Kolluri is the managing member of NTP SPV I and therefore may be deemed to have voting and dispositive power over the shares held by NT SPV I. Kolluri disclaims beneficial ownership of the shares held by NT SPV I except to the extent of his pecuniary interest therein.

(8) The shares were obtained pursuant to a pro-rata, in-kind distribution from NT SPV I, for no additional consideration.

(9) The reported security is directly held by NTP SPV I. Kolluri is the managing member of NTP SPV I and therefore may be deemed to have voting and dispositive power over the shares held by NTP SPV I. Kolluri disclaims beneficial ownership of the shares held by NTP SPV I except to the extent of his pecuniary interest therein.

(10) Represents a pro-rata, in-kind distribution by NTP SPV I to its members and assignees, for no additional consideration.

(11) Represents a pro-rata, in-kind distribution by NeoTribe Ventures I, L.P. ("NTV I") to its partners, for no additional consideration.

(12) The reported security is directly held by NTV I, for itself. NeoTribe Partners I, LLC ("NTP I") is the general partner of NTV I. Kolluri is the managing member of NTP I and therefore may be deemed to hold voting and dispositive power over the shares held by NTV I. Kolluri disclaims beneficial ownership of the shares held by NTV I except to the extent of his pecuniary interest therein.

(13) The shares were obtained pursuant to a pro-rata, in-kind distribution from NTV I, for no additional consideration.

(14) The reported security is directly held by NTP I. Kolluri is the managing member of NTP I and therefore may be deemed to have voting and dispositive power over the shares held by NTP I. Kolluri disclaims beneficial ownership of the shares held by NTP I except to the extent of his pecuniary interest therein.

(15) Represents a pro-rata, in-kind distribution by NTP I to its members and assignees, for no additional consideration.

(16) Represents a pro-rata, in-kind distribution by NeoTribe Associates I, L.P. ("NTA I") to its partners, for no additional consideration.

(17) The reported security is directly held by NTV I, as nominee for NTA I. NTP I is the general partner of NTA I. Kolluri is the managing member of NTP I and therefore may be deemed to hold voting and dispositive power over the shares held by NTA I. Kolluri disclaims beneficial ownership of the shares held by NTA I except to the extent of his pecuniary interest therein.

(18) The reported security is directly held by Ishaan S. Kolluri GST Exempt Trust under the Kolluri 2012 Trust Dtd 11/16/2012, of which Kolluri is a trustee and therefore may be deemed to hold voting and dispositive power over these shares. Kolluri disclaims beneficial ownership of these shares.

(19) The reported security is directly held by Raina V. Kolluri GST Exempt Trust under the Kolluri 2012 Trust Dtd 11/16/2012, of which Kolluri is a trustee and therefore may be deemed to hold voting and dispositive power over these shares. Kolluri disclaims beneficial ownership of these shares.

(20) The shares were obtained pursuant to a pro-rata, in-kind distribution from NTIP I, for no additional consideration.

(21) The reported security is directly held by Kolluri Living Trust dated 11/05/1999, of which Kolluri is a trustee and therefore may be deemed to hold voting and dispositive power over these shares. Kolluri disclaims beneficial ownership of these shares except to the extent of his pecuniary interest therein.

(22) The shares were obtained pursuant to a pro-rata, in-kind distribution from NTP SPV I, for no additional consideration.

(23) The shares were obtained pursuant to a pro-rata, in-kind distribution from NTP I, for no additional consideration.

(24) The shares were obtained pursuant to a pro-rata, in-kind distribution from NTA I, for no additional consideration.

**Remarks:**
This Form 4 is one of two Form 4s filed in respect of the distribution of these shares held of record by NTIF I, NT SPV I, NTV I and NTA I. The Reporting Person for the other Form 4 is NeoTribe Ventures I, L.P.

**Signature:** Krishna Kittu Kolluri, /s/ Krishna Kittu Kolluri  
**Date:** 2026-05-13

### Remarks:

Reminder: Report on a separate line for each class of securities beneficially owned directly or indirectly.

* If the form is filed by more than one reporting person, see Instruction 4 (b)(v).

** Intentional misstatements or omissions of facts constitute Federal Criminal Violations See 18 U.S.C. 1001 and 15 U.S.C. 78ff(a).

Note: File three copies of this Form, one of which must be manually signed. If space is insufficient, see Instruction 6 for procedure.

**Persons who respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB Number.**