# EDGAR Filing Document

**Accession Number:** 0000906185
**File Stem:** 0001193125-25-301801
**Filing Date:** 2025-11
**Character Count:** 45557
**Document Hash:** bd20f97824f465039ca6c694a3e76b81
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-301801.hdr.sgml**: 20251128

**ACCESSION NUMBER**: 0001193125-25-301801

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 2

**CONFORMED PERIOD OF REPORT**: 20250930

**FILED AS OF DATE**: 20251128

**DATE AS OF CHANGE**: 20251128

**PERIOD START**: 20251231

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS ASPEN SERIES
- **CENTRAL INDEX KEY:** 0000906185

**ORGANIZATION NAME:**
- **EIN:** 841235540
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-07736
- **FILM NUMBER:** 251534205

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 3033333863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

## Series and Classes Contracts Data

### Janus Henderson Global Sustainable Equity Portfolio (Series ID: S000074910)

| Class ID   | Class Name           | Ticker Symbol   |
|:---|:---|:---|
| C000233309 | Institutional Shares | JHISX           |
| C000233310 | Service Shares       |  |

## Nport-Ex

**Janus Henderson VIT Global Sustainable Equity Portfolio**

**Schedule of Investments (unaudited)**

**September 30, 2025**

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | |
|:---|:---|:---|
|  | *Shares* | *Value* |
| Common Stocks – 97.8% |  |  |
| Auto Components – 1.8% |  |  |
| &nbsp;&nbsp;&nbsp; Aptive PLC<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 2862 | &nbsp;&nbsp;&nbsp;&nbsp; $246762 |
| Banks – 1.5% |  |  |
| &nbsp;&nbsp;&nbsp; HDFC Bank Ltd (ADR) | &nbsp;&nbsp;&nbsp;&nbsp; 6283 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 214627 |
| Biotechnology – 2.8% |  |  |
| &nbsp;&nbsp;&nbsp; Argenx SE (ADR)<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 370 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 272897 |
| &nbsp;&nbsp;&nbsp; Vertex Pharmaceuticals Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 295 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 115534 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 388431 |
| Building Products – 3.7% |  |  |
| &nbsp;&nbsp;&nbsp; Advanced Drainage Systems Inc | &nbsp;&nbsp;&nbsp;&nbsp; 1187 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 164637 |
| &nbsp;&nbsp;&nbsp; Cie de Saint-Gobain | &nbsp;&nbsp;&nbsp;&nbsp; 3261 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 355210 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 519847 |
| Capital Markets – 3.2% |  |  |
| &nbsp;&nbsp;&nbsp; Intercontinental Exchange Inc | &nbsp;&nbsp;&nbsp;&nbsp; 1621 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 273106 |
| &nbsp;&nbsp;&nbsp; S&P Global Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 369 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 179596 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 452702 |
| Commercial Services & Supplies – 1.0% |  |  |
| &nbsp;&nbsp;&nbsp; Tetra Tech Inc | &nbsp;&nbsp;&nbsp;&nbsp; 4309 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 143834 |
| Construction & Engineering – 3.0% |  |  |
| &nbsp;&nbsp;&nbsp; APi Group Corp<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 6269 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 215465 |
| &nbsp;&nbsp;&nbsp; Stantec Inc | &nbsp;&nbsp;&nbsp;&nbsp; 1960 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 211410 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 426875 |
| Diversified Financial Services – 3.2% |  |  |
| &nbsp;&nbsp;&nbsp; Mastercard Inc - Class A | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 576 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 327634 |
| &nbsp;&nbsp;&nbsp; Walker & Dunlop Inc | &nbsp;&nbsp;&nbsp;&nbsp; 1427 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 119326 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 446960 |
| Electrical Equipment – 10.0% |  |  |
| &nbsp;&nbsp;&nbsp; Hubbell Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 497 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 213864 |
| &nbsp;&nbsp;&nbsp; Legrand SA | &nbsp;&nbsp;&nbsp;&nbsp; 1200 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 200148 |
| &nbsp;&nbsp;&nbsp; NEXTracker Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 1525 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 112835 |
| &nbsp;&nbsp;&nbsp; Prysmian SpA | &nbsp;&nbsp;&nbsp;&nbsp; 4629 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 462305 |
| &nbsp;&nbsp;&nbsp; Schneider Electric SE | &nbsp;&nbsp;&nbsp;&nbsp; 1464 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 413243 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 1402395 |
| Electronic Equipment, Instruments & Components – 4.5% |  |  |
| &nbsp;&nbsp;&nbsp; Keyence Corp | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 200 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 74474 |
| &nbsp;&nbsp;&nbsp; Keysight Technologies Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 1191 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 208330 |
| &nbsp;&nbsp;&nbsp; TE Connectivity PLC | &nbsp;&nbsp;&nbsp;&nbsp; 1574 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 345540 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 628344 |
| Entertainment – 4.6% |  |  |
| &nbsp;&nbsp;&nbsp; Nintendo Co Ltd | &nbsp;&nbsp;&nbsp;&nbsp; 1800 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 154215 |
| &nbsp;&nbsp;&nbsp; Spotify Technology SA<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 707 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 493486 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 647701 |
| Food Products – 0.2% |  |  |
| &nbsp;&nbsp;&nbsp; McCormick & Co Inc/MD | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 512 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 34258 |
| Health Care Providers & Services – 3.4% |  |  |
| &nbsp;&nbsp;&nbsp; McKesson Corp | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 615 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 475112 |
| Independent Power and Renewable Electricity Producers – 1.2% |  |  |
| &nbsp;&nbsp;&nbsp; Boralex Inc - Class A | &nbsp;&nbsp;&nbsp;&nbsp; 8564 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 167218 |
| Insurance – 11.5% |  |  |
| &nbsp;&nbsp;&nbsp; AIA Group Ltd | &nbsp;&nbsp; 45600 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 437101 |
| &nbsp;&nbsp;&nbsp; Arthur J Gallagher & Co | &nbsp;&nbsp;&nbsp;&nbsp; 1404 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 434875 |
| &nbsp;&nbsp;&nbsp; Intact Financial Corp | &nbsp;&nbsp;&nbsp;&nbsp; 1301 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 253160 |
| &nbsp;&nbsp;&nbsp; Marsh & McLennan Cos Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 870 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 175331 |
| &nbsp;&nbsp;&nbsp; Progressive Corp/The | &nbsp;&nbsp;&nbsp;&nbsp; 1302 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 321529 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 1621996 |
| Leisure Products – 0.4% |  |  |
| &nbsp;&nbsp;&nbsp; Shimano Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 500 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 56817 |
| Machinery – 4.4% |  |  |
| &nbsp;&nbsp;&nbsp; Knorr-Bremse AG | &nbsp;&nbsp;&nbsp;&nbsp; 1849 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 173614 |
| &nbsp;&nbsp;&nbsp; Wabtec Corp | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 879 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 176213 |
| &nbsp;&nbsp;&nbsp; Xylem Inc/NY | &nbsp;&nbsp;&nbsp;&nbsp; 1799 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 265353 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 615180 |
| Pharmaceuticals – 1.6% |  |  |
| &nbsp;&nbsp;&nbsp; Eli Lilly & Co | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 295 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 225085 |

---

------

---

| | | |
|:---|:---|:---|
|  | *Shares* | *Value* |
| Common Stocks – (continued) |  |  |
| Professional Services – 3.6% |  |  |
| &nbsp;&nbsp;&nbsp; Experian PLC | &nbsp;&nbsp;&nbsp;&nbsp; 6587 | &nbsp;&nbsp;&nbsp;&nbsp; $331687 |
| &nbsp;&nbsp;&nbsp; Wolters Kluwer NV | &nbsp;&nbsp;&nbsp;&nbsp; 1328 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 181259 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 512946 |
| Road & Rail – 2.4% |  |  |
| &nbsp;&nbsp;&nbsp; Uber Technologies Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 3374 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 330551 |
| Semiconductor & Semiconductor Equipment – 13.7% |  |  |
| &nbsp;&nbsp;&nbsp; ASML Holding NV | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 87 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 84811 |
| &nbsp;&nbsp;&nbsp; Disco Corp | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 31417 |
| &nbsp;&nbsp;&nbsp; Infineon Technologies AG | &nbsp;&nbsp;&nbsp;&nbsp; 3642 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 142136 |
| &nbsp;&nbsp;&nbsp; KLA Corp | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 183 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 197384 |
| &nbsp;&nbsp;&nbsp; NVIDIA Corp | &nbsp;&nbsp;&nbsp;&nbsp; 5175 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 965551 |
| &nbsp;&nbsp;&nbsp; Taiwan Semiconductor Manufacturing Co Ltd (ADR) | &nbsp;&nbsp;&nbsp;&nbsp; 1791 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 500208 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 1921507 |
| Software – 12.7% |  |  |
| &nbsp;&nbsp;&nbsp; Autodesk Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 919 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 291939 |
| &nbsp;&nbsp;&nbsp; Cadence Design Systems Inc<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 766 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 269065 |
| &nbsp;&nbsp;&nbsp; Fair Isaac Corp<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 41 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 61358 |
| &nbsp;&nbsp;&nbsp; Microsoft Corp | &nbsp;&nbsp;&nbsp;&nbsp; 1843 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 954582 |
| &nbsp;&nbsp;&nbsp; SAP SE | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 800 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 214446 |
|  |  | &nbsp;&nbsp;&nbsp;&nbsp; 1791390 |
| Specialty Retail – 1.3% |  |  |
| &nbsp;&nbsp;&nbsp; Home Depot Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 434 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 175852 |
| Trading Companies & Distributors – 1.1% |  |  |
| &nbsp;&nbsp;&nbsp; Core & Main Inc - Class A<sup>\*</sup> <br>| &nbsp;&nbsp;&nbsp;&nbsp; 2887 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 155407 |
| Wireless Telecommunication Services – 1.0% |  |  |
| &nbsp;&nbsp;&nbsp; T-Mobile US Inc | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 596 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 142671 |
| Total Common Stocks (cost $10,561,028) |  | &nbsp;&nbsp; 13744468 |
| Investment Companies – 2.6% |  |  |
| Money Markets – 2.6% |  |  |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº,</sup><sup>£</sup> (cost $368,375) | 368301 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 368375 |
| Total Investments (total cost $10,929,403) – 100.4% |  | &nbsp;&nbsp; 14112843 |
| Liabilities, net of Cash, Receivables and Other Assets – (0.4)% |  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (53247) |
| Net Assets – 100% |  | $14059596 |

---

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | |
|:---|:---|:---|
| ***Summary of Investments by Country - (Long Positions) (unaudited)*** |  |  |
| *Country* | *Value* | &nbsp;&nbsp; *% of* <br>*Investment* <br>*Securities*<br>|
| United States | $8341414 | &nbsp;&nbsp;&nbsp;&nbsp; 59.1<br> %<br>|
| France | 968601 | &nbsp;&nbsp;&nbsp;&nbsp;6.9 |
| Canada | 631788 | &nbsp;&nbsp;&nbsp;&nbsp;4.5 |
| Germany | 530196 | &nbsp;&nbsp;&nbsp;&nbsp;3.8 |
| Taiwan | 500208 | &nbsp;&nbsp;&nbsp;&nbsp;3.5 |
| Sweden | 493486 | &nbsp;&nbsp;&nbsp;&nbsp;3.5 |
| Italy | 462305 | &nbsp;&nbsp;&nbsp;&nbsp;3.3 |
| Hong Kong | 437101 | &nbsp;&nbsp;&nbsp;&nbsp;3.1 |
| Ireland | 345540 | &nbsp;&nbsp;&nbsp;&nbsp;2.4 |
| United Kingdom | 331687 | &nbsp;&nbsp;&nbsp;&nbsp;2.4 |
| Japan | 316923 | &nbsp;&nbsp;&nbsp;&nbsp;2.2 |
| Belgium | 272897 | &nbsp;&nbsp;&nbsp;&nbsp;1.9 |
| Netherlands | 266070 | &nbsp;&nbsp;&nbsp;&nbsp;1.9 |
| India | 214627 | &nbsp;&nbsp;&nbsp;&nbsp;1.5 |
| Total | $14112843 | &nbsp;&nbsp;&nbsp;&nbsp; 100.0<br> %<br>|

---

------

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| | | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|:---|
| ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** | ***Schedule of Affiliated Investments - (% of Net Assets)*** |
| *Affiliate* | *Affiliated* <br>*Investments,* <br>*at Value at* <br>*12/31/24*<br>| *Purchases* | *Sales* <br>*Proceeds*<br>| *Realized* <br>*Gain/(Loss)*<br>| *Change in* <br>*Unrealized* <br>*Appreciation/* <br>*Depreciation*<br>| *Affiliated* <br>*Investments,* <br>*at Value at* <br>*9/30/25*<br>| *Ending* <br>*Shares*<br>| *Dividend* <br>*Income*<br>|
| Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% | Investment Companies - 2.6% |
| Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% | Money Markets - 2.6% |
| &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  | &nbsp;&nbsp;&nbsp; Janus Henderson Cash Liquidity Fund LLC, 4.0538%<sup>ºº</sup>  |
|  | &nbsp;&nbsp; $427510 | &nbsp;&nbsp; $8388837 | &nbsp;&nbsp; $(8447965) | &nbsp;&nbsp; $(7) | &nbsp;&nbsp; $- | &nbsp;&nbsp; $368375 | &nbsp;&nbsp; 368301 | &nbsp;&nbsp; $19223 |

---

------

**Notes to Schedule of Investments (unaudited)** 

ADR American Depositary Receipt <br> LLC Limited Liability Company <br> PLC Public Limited Company

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;

---

| |
|:---|
| Non-income producing security. |
| Rate shown is the 7-day yield as of September 30, 2025. |
| £&nbsp;&nbsp; The Portfolio may invest in certain securities that are considered affiliated companies. As defined by the Investment Company Act of 1940, <br> as amended, an affiliated company is one in which the Portfolio owns 5% or more of the outstanding voting securities, or a company which <br> is under common ownership or control.<br>|

---

The following is a summary of the inputs that were used to value the Portfolio's investments in securities and other

financial instruments as of September 30, 2025.

---

| | | | |
|:---|:---|:---|:---|
| **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** | **Valuation Inputs Summary** |
|  | ***Level 1 -*** <br>***Quoted Prices***<br>| &nbsp;&nbsp; ***Level 2 -*** <br>***Other Significant*** <br>***Observable Inputs***<br>| &nbsp;&nbsp; ***Level 3 -*** <br>***Significant*** <br>***Unobservable Inputs***<br>|
| **Assets** |  |  |  |
| **Investments In Securities:** |  |  |  |
| *Common Stocks* |  |  |  |
| Building Products | $164637 | &nbsp;&nbsp;&nbsp; $355210 | &nbsp;&nbsp;&nbsp; $- |
| Electrical Equipment | 326699 | &nbsp;&nbsp;&nbsp; 1075696 | &nbsp;&nbsp;&nbsp; - |
| Electronic Equipment, Instruments & Components | 553870 | &nbsp;&nbsp;&nbsp; 74474 | &nbsp;&nbsp;&nbsp; - |
| Entertainment | 493486 | &nbsp;&nbsp;&nbsp; 154215 | &nbsp;&nbsp;&nbsp; - |
| Insurance | 1184895 | &nbsp;&nbsp;&nbsp; 437101 | &nbsp;&nbsp;&nbsp; - |
| Leisure Products | - | &nbsp;&nbsp;&nbsp; 56817 | &nbsp;&nbsp;&nbsp; - |
| Machinery | 441566 | &nbsp;&nbsp;&nbsp; 173614 | &nbsp;&nbsp;&nbsp; - |
| Professional Services | - | &nbsp;&nbsp;&nbsp; 512946 | &nbsp;&nbsp;&nbsp; - |
| Semiconductor & Semiconductor Equipment | 1663143 | &nbsp;&nbsp;&nbsp; 258364 | &nbsp;&nbsp;&nbsp; - |
| Software | 1576944 | &nbsp;&nbsp;&nbsp; 214446 | &nbsp;&nbsp;&nbsp; - |
| All Other | 4026345 | &nbsp;&nbsp;&nbsp; - | &nbsp;&nbsp;&nbsp; - |
| *Investment Companies* | - | &nbsp;&nbsp;&nbsp; 368375 | &nbsp;&nbsp;&nbsp; - |
| **Total Assets** | $10431585 | &nbsp;&nbsp;&nbsp; $3681258 | &nbsp;&nbsp;&nbsp; $- |

---

------

**Investment Valuation**

Portfolio holdings are valued in accordance with policies and procedures established by the Adviser pursuant to Rule 2a-5 under the 1940 Act and approved by and subject to the oversight of the Trustees (the "Valuation Procedures"). Equity securities traded on a domestic securities exchange are generally valued at readily available market quotations, which are (i) the official close prices or (ii) last sale prices on the primary market or exchange in which the securities trade. If such price is lacking for the trading period immediately preceding the time of determination, such securities are generally valued at their current bid price. Equity securities that are traded on a foreign exchange are generally valued at the closing prices on such markets. In the event that there is no current trading volume on a particular security in such foreign exchange, the bid price from the primary exchange is generally used to value the security. Foreign securities and currencies are converted to U.S. dollars using the current spot USD dollar exchange rate in effect at the close of the New York Stock Exchange ("NYSE"). The Adviser will determine the market value of individual securities held by it by using prices provided by one or more Adviser-approved professional pricing services or, as needed, by obtaining market quotations from independent broker-dealers. Most debt securities are valued in accordance with the evaluated bid price supplied by the pricing service that is intended to reflect market value. The evaluated bid price supplied by the pricing service is an evaluation that may consider factors such as security prices, yields, maturities, and ratings. Certain short-term securities maturing within 60 days or less may be evaluated and valued on an amortized cost basis provided that the amortized cost determined approximates market value. Securities for which market quotations or evaluated prices are not readily available or deemed unreliable are valued at fair value determined in good faith by the Adviser pursuant to the Valuation Procedures. Circumstances in which fair valuation may be utilized include, but are not limited to: (i) a significant event that may affect the securities of a single issuer, such as a merger, bankruptcy, or significant issuer-specific development; (ii) an event that may affect an entire market, such as a natural disaster or significant governmental action; (iii) a nonsignificant event such as a market closing early or not opening, or a security trading halt; and (iv) pricing of a non-valued security and a restricted or nonpublic security. Special valuation considerations may apply with respect to "odd-lot" fixed-income transactions which, due to their small size, may receive evaluated prices by pricing services which reflect a large block trade and not what actually could be obtained for the odd-lot position. The value of the securities of other mutual funds held by the Portfolio, if any, will be calculated using the NAV of such mutual funds, and the prospectuses for such mutual funds explain the circumstances under which they use fair valuation and the effects of using fair valuation. The value of the securities of any cash management pooled investment vehicles that operate as money market funds held by the Portfolio, if any, will be calculated using the NAV of

such funds.

**Valuation Inputs Summary**

FASB ASC 820, *Fair Value Measurements and Disclosures* ("ASC 820"), defines fair value, establishes a framework for measuring fair value, and expands disclosure requirements regarding fair value measurements. This standard emphasizes that fair value is a market-based measurement that should be determined based on the assumptions that market participants would use in pricing an asset or liability and establishes a hierarchy that prioritizes inputs to valuation techniques used to measure fair value. These inputs are summarized into three broad levels:

Level 1 – Unadjusted quoted prices in active markets the Portfolio has the ability to access for identical assets or liabilities.

Level 2 – Observable inputs other than unadjusted quoted prices included in Level 1 that are observable for the asset or liability either directly or indirectly. These inputs may include quoted prices for the identical instrument on an inactive market, prices for similar instruments, interest rates, prepayment speeds, credit risk, yield curves, default

rates and similar data.

Assets or liabilities categorized as Level 2 in the hierarchy generally include: debt securities fair valued in accordance with the evaluated bid or ask prices supplied by a pricing service; securities traded on OTC markets and listed securities for which no sales are reported that are fair valued at the latest bid price (or yield equivalent thereof) obtained from one or more dealers transacting in a market for such securities or by a pricing service approved by the Portfolio's Trustees; certain short-term debt securities with maturities of 60 days or less that are fair valued at amortized cost; and equity securities of foreign issuers whose fair value is determined by using systematic fair valuation models provided by independent third parties in order to adjust for stale pricing which may occur between the close of certain foreign exchanges and the close of the NYSE. Other securities that may be categorized as Level 2 in the hierarchy include, but are not limited to, preferred stocks, bank loans, swaps,

investments in unregistered investment companies, options, and forward contracts.

Level 3 – Unobservable inputs for the asset or liability to the extent that relevant observable inputs are not available, representing the Portfolio's own assumptions about the assumptions that a market participant would use in valuing the asset or liability, and that would be based on the best information available.

There have been no significant changes in valuation techniques used in valuing any such positions held by the Portfolio since the beginning of the fiscal period.

------

The inputs or methodology used for fair valuing securities are not necessarily an indication of the risk associated with investing in those securities. The summary of inputs used as of September 30, 2025 to fair value the Portfolio's investments in securities and other financial instruments is included in the "Valuation Inputs Summary" in the Notes to Schedule of Investments.

------

For additional information on the Portfolio, please refer to the Portfolio's most recent semiannual or annual financial

statements.

109-35-70453 11-25

------

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS ASPEN SERIES

- **b. Investment Company Act file number:** 811-07736

- **c. CIK number of Registrant:** 0000906185

- **d. LEI of Registrant:** 5C1H794C69TI10A0XC04

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 DETROIT STREET

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Global Sustainable Equity Portfolio

- **b. EDGAR series identifier (if any):** S000074910

- **c. LEI of Series:** 549300C08BPTT85GZO74

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-12-31

- **b. Date as of which information is reported:** 2025-09-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $14129747.33

**Total Liabilities:** $70150.94

**Net Assets:** $14059596.39

**Cash Not Reported:** $2064.81

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000233309 | 0.00%                | 0.30%                | 2.79%                |
| Class ID C000233310 | 0.00%                | 0.30%                | 2.72%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $203377.00               | $-216056.00                                |
| Month 2  | $-7073.00                | $52473.00                                  |
| Month 3  | $196033.00               | $161667.00                                 |

**Designated Index Information**

- **Index Name:** MSCI The World Index

- **Index Identifier:** MSCI.MC.MXWO

### Schedule of Portfolio Investments

| Name                                         | Title                                                                 | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Core & Main Inc                              | Core & Main Inc CL A                                                  | CUSIP: 21874C102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2887 | NS      | $155407.21    | 1.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Stantec Inc.                                 | Stantec Inc. COM NPV                                                  | CUSIP: 85472N109<br>LEI: 549300MZ7NGUZDCP2T16 | Long             | EC               | CORP              | CA        |      1960 | NS      | $211409.56    | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Fair Isaac Corporation                       | Fair Isaac Corporation COM USD0.01                                    | CUSIP: 303250104<br>LEI: RI6HET5SJUEY30V1PS26 | Long             | EC               | CORP              | US        |        41 | NS      | $61357.73     | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| HDFC Bank Ltd.                               | HDFC Bank Ltd. SPON ADS EACH REP 3 ORD SHS                            | CUSIP: 40415F101<br>LEI: 335800ZQ6I4E2JXENC50 | Long             |  | CORP              | US        |      6283 | NS      | $214627.28    | 1.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Home Depot Inc.                              | Home Depot Inc. COM USD0.05                                           | CUSIP: 437076102<br>LEI: QEKMOTMBBKA8I816DO57 | Long             | EC               | CORP              | US        |       434 | NS      | $175852.46    | 1.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Hubbell Incorporated                         | Hubbell Incorporated COM USD0.001                                     | CUSIP: 443510607<br>LEI: 54930088VDQ6840Y6597 | Long             | EC               | CORP              | US        |       497 | NS      | $213864.07    | 1.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Intercontinental Exchange Inc                | Intercontinental Exchange Inc COM USD0.01                             | CUSIP: 45866F104<br>LEI: 5493000F4ZO33MV32P92 | Long             | EC               | CORP              | US        |      1621 | NS      | $273106.08    | 1.94%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                    | KLA Corp. COM USD0.001                                                | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |       183 | NS      | $197383.80    | 1.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Schneider Electric SE                        | Schneider Electric SE EUR4.00                                         | CUSIP: F86921107<br>LEI: 969500A1YF1XUYYXS284 | Long             | EC               | CORP              | FR        |      1464 | NS      | $413242.88    | 2.94%             |  |  |  | No            |                  2 | On Loan: No      |
| SAP SE                                       | SAP SE ORD NPV                                                        | CUSIP: D66992104<br>LEI: 529900D6BF99LW9R2E68 | Long             | EC               | CORP              | DE        |       800 | NS      | $214446.13    | 1.53%             |  |  |  | No            |                  2 | On Loan: No      |
| Keysight Technologies, Inc.                  | Keysight Technologies, Inc. COM USD0.01 WD                            | CUSIP: 49338L103<br>LEI: 549300GLKVIO8YRCYN02 | Long             | EC               | CORP              | US        |      1191 | NS      | $208329.72    | 1.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly & Co.                              | Eli Lilly & Co. COM NPV                                               | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |       295 | NS      | $225085.00    | 1.60%             |  |  |  | No            |                  1 | On Loan: No      |
| Wolters Kluwer NV                            | Wolters Kluwer NV EUR0.12                                             | CUSIP: N9643A197<br>LEI: 724500TEM53I0U077B74 | Long             | EC               | CORP              | NL        |      1328 | NS      | $181259.20    | 1.29%             |  |  |  | No            |                  2 | On Loan: No      |
| Marsh & McLennan Companies, Inc.             | Marsh & McLennan Companies, Inc. COM USD1.00                          | CUSIP: 571748102<br>LEI: 549300XMP3KDCKJXIU47 | Long             | EC               | CORP              | US        |       870 | NS      | $175331.10    | 1.25%             |  |  |  | No            |                  1 | On Loan: No      |
| MasterCard Incorporated                      | MasterCard Incorporated COM USD0.0001 CLASS A                         | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |       576 | NS      | $327634.56    | 2.33%             |  |  |  | No            |                  1 | On Loan: No      |
| McCormick & Company, Incorporated            | McCormick & Company, Incorporated COM NPV                             | CUSIP: 579780206<br>LEI: 549300JQQA6MQ4OJP259 | Long             | EC               | CORP              | US        |       512 | NS      | $34257.92     | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Mckesson Corp.                               | Mckesson Corp. COM USD0.01                                            | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |       615 | NS      | $475112.10    | 3.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Infineon Technologies AG                     | Infineon Technologies AG ORD NPV (REGD)                               | CUSIP: D35415104<br>LEI: TSI2PJM6EPETEQ4X1U25 | Long             | EC               | CORP              | DE        |      3642 | NS      | $142135.53    | 1.01%             |  |  |  | No            |                  2 | On Loan: No      |
| Microsoft Corp.                              | Microsoft Corp. COM USD0.00000625                                     | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |      1843 | NS      | $954581.85    | 6.79%             |  |  |  | No            |                  1 | On Loan: No      |
| DISCO Corp.                                  | DISCO Corp. NPV                                                       | CUSIP: J12327102<br>LEI: N/A                  | Long             | EC               | CORP              | JP        |       100 | NS      | $31416.94     | 0.22%             |  |  |  | No            |                  2 | On Loan: No      |
| Keyence Corp.                                | Keyence Corp. NPV                                                     | CUSIP: J32491102<br>LEI: 529900M7PQBS7A89DZ11 | Long             | EC               | CORP              | JP        |       200 | NS      | $74473.86     | 0.53%             |  |  |  | No            |                  2 | On Loan: No      |
| Nextracker Inc.                              | Nextracker Inc. CLASS A COM                                           | CUSIP: 65290E101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1525 | NS      | $112834.75    | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Nintendo Co Ltd.                             | Nintendo Co Ltd. NPV                                                  | CUSIP: J51699106<br>LEI: 353800FEEXU6I9M0ZF27 | Long             | EC               | CORP              | JP        |      1800 | NS      | $154215.07    | 1.10%             |  |  |  | No            |                  2 | On Loan: No      |
| NVIDIA Corp.                                 | NVIDIA Corp. COM USD0.001                                             | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |      5175 | NS      | $965551.50    | 6.87%             |  |  |  | No            |                  1 | On Loan: No      |
| Shimano Inc.                                 | Shimano Inc. NPV                                                      | CUSIP: J72262108<br>LEI: 529900L32YT3P9DECR10 | Long             | EC               | CORP              | JP        |       500 | NS      | $56816.75     | 0.40%             |  |  |  | No            |                  2 | On Loan: No      |
| The Progressive Corporation                  | The Progressive Corporation COM USD1.00                               | CUSIP: 743315103<br>LEI: 529900TACNVLY9DCR586 | Long             | EC               | CORP              | US        |      1302 | NS      | $321528.90    | 2.29%             |  |  |  | No            |                  1 | On Loan: No      |
| S&P Global Inc                               | S&P Global Inc COM USD1                                               | CUSIP: 78409V104<br>LEI: Y6X4K52KMJMZE7I7MY94 | Long             | EC               | CORP              | US        |       369 | NS      | $179595.99    | 1.28%             |  |  |  | No            |                  1 | On Loan: No      |
| T-Mobile US Inc                              | T-Mobile US Inc COM USD0.00001                                        | CUSIP: 872590104<br>LEI: 549300QHIJYOHPACPG31 | Long             | EC               | CORP              | US        |       596 | NS      | $142670.48    | 1.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Co., Ltd. | Taiwan Semiconductor Manufacturing Co., Ltd. SPON ADS REP 5 ORD TWD10 | CUSIP: 874039100<br>LEI: 549300KB6NK5SBD14S87 | Long             |  | CORP              | US        |      1791 | NS      | $500208.39    | 3.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Tetra Tech Inc                               | Tetra Tech Inc COM USD0.01                                            | CUSIP: 88162G103<br>LEI: 549300UJ1LP7JGJC5P32 | Long             | EC               | CORP              | US        |      4309 | NS      | $143834.42    | 1.02%             |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC                      | Janus Henderson Cash Liquidity Fund LLC                               | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |    368301 | NS      | $368374.97    | 2.62%             |  |  |  | No            |                  2 | On Loan: No      |
| Uber Technologies Inc                        | Uber Technologies Inc COM USD0.00001                                  | CUSIP: 90353T100<br>LEI: 549300B2FTG34FILDR98 | Long             | EC               | CORP              | US        |      3374 | NS      | $330550.78    | 2.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated          | Vertex Pharmaceuticals Incorporated COM USD0.01                       | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |       295 | NS      | $115533.80    | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Wabtec Corp                                  | Wabtec Corp COM USD0.01                                               | CUSIP: 929740108<br>LEI: 06BTX5UWZD0GQ5N5Y745 | Long             | EC               | CORP              | US        |       879 | NS      | $176213.13    | 1.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Walker & Dunlop Inc.                         | Walker & Dunlop Inc. COM USD0.01                                      | CUSIP: 93148P102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1427 | NS      | $119325.74    | 0.85%             |  |  |  | No            |                  1 | On Loan: No      |
| Intact Financial Corp.                       | Intact Financial Corp. COM NPV                                        | CUSIP: 45823T106<br>LEI: 5493009LXGE7Y4U9OY43 | Long             | EC               | CORP              | CA        |      1301 | NS      | $253159.73    | 1.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Legrand SA                                   | Legrand SA EUR4                                                       | CUSIP: F56196185<br>LEI: 969500XXRPGD7HCAFA90 | Long             | EC               | CORP              | FR        |      1200 | NS      | $200148.28    | 1.42%             |  |  |  | No            |                  2 | On Loan: No      |
| Experian PLC                                 | Experian PLC ORD USD0.10                                              | CUSIP: G32655105<br>LEI: 635400RXXHHYT1JTSZ26 | Long             | EC               | CORP              | GB        |      6587 | NS      | $331687.12    | 2.36%             |  |  |  | No            |                  2 | On Loan: No      |
| Prysmian S.p.A.                              | Prysmian S.p.A. NPV                                                   | CUSIP: T7630L105<br>LEI: 529900X0H1IO3RS1A464 | Long             | EC               | CORP              | IT        |      4629 | NS      | $462305.07    | 3.29%             |  |  |  | No            |                  2 | On Loan: No      |
| Knorr-Bremse AG                              | Knorr-Bremse AG NPV                                                   | CUSIP: D4S43E114<br>LEI: 5299001GRRO0Z25YZT52 | Long             | EC               | CORP              | DE        |      1849 | NS      | $173614.35    | 1.23%             |  |  |  | No            |                  2 | On Loan: No      |
| TE Connectivity PLC                          | TE Connectivity PLC ORD SHS                                           | CUSIP: G87052109<br>LEI: 6367003TB6K484NFHE54 | Long             | EC               | CORP              | US        |      1574 | NS      | $345540.22    | 2.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Spotify Technology S.A.                      | Spotify Technology S.A. COM EUR0.000625                               | CUSIP: L8681T102<br>LEI: 549300B4X0JHWV0DTD60 | Long             | EC               | CORP              | US        |       707 | NS      | $493486.00    | 3.51%             |  |  |  | No            |                  1 | On Loan: No      |
| API Group Corp                               | API Group Corp COM STK                                                | CUSIP: 00187Y100<br>LEI: 213800NZT1VX6PZ7BT53 | Long             | EC               | CORP              | US        |      6269 | NS      | $215465.53    | 1.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Advanced Drainage System Inc                 | Advanced Drainage System Inc COM USD0.01                              | CUSIP: 00790R104<br>LEI: 6QQTOZ5MF5DHM7K92I60 | Long             | EC               | CORP              | US        |      1187 | NS      | $164636.90    | 1.17%             |  |  |  | No            |                  1 | On Loan: No      |
| AIA Group Ltd, Hong Kong                     | AIA Group Ltd, Hong Kong NPV                                          | CUSIP: Y002A1105<br>LEI: ZP5ILWVSYE4LJGMMVD57 | Long             | EC               | CORP              | HK        |     45600 | NS      | $437100.92    | 3.11%             |  |  |  | No            |                  2 | On Loan: No      |
| ASML Holding N.V.                            | ASML Holding N.V. EUR0.09                                             | CUSIP: N07059202<br>LEI: 724500Y6DUVHQD6OXN27 | Long             | EC               | CORP              | NL        |        87 | NS      | $84810.97     | 0.60%             |  |  |  | No            |                  2 | On Loan: No      |
| Argenx SE                                    | Argenx SE SPON ADR EACH REP 1 ORD SHS                                 | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |       370 | NS      | $272897.20    | 1.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Gallagher Aj &Co                             | Gallagher Aj &Co COM USD1.00                                          | CUSIP: 363576109<br>LEI: 54930049QLLMPART6V29 | Long             | EC               | CORP              | US        |      1404 | NS      | $434874.96    | 3.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Aptiv PLC                                    | Aptiv PLC COM SHS                                                     | CUSIP: G3265R107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2862 | NS      | $246761.64    | 1.76%             |  |  |  | No            |                  1 | On Loan: No      |
| Autodesk Inc                                 | Autodesk Inc COM USD0.01                                              | CUSIP: 052769106<br>LEI: FRKKVKAIQEF3FCSTPG55 | Long             | EC               | CORP              | US        |       919 | NS      | $291938.73    | 2.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Cadence Design Systems, Inc.                 | Cadence Design Systems, Inc. COM USD0.01                              | CUSIP: 127387108<br>LEI: GCT7RXJOGLXPV0NXZY22 | Long             | EC               | CORP              | US        |       766 | NS      | $269065.16    | 1.91%             |  |  |  | No            |                  1 | On Loan: No      |
| Boralex Inc                                  | Boralex Inc CL'A'COM NPV                                              | CUSIP: 09950M300<br>LEI: 5493004UT7HNQU2SVR90 | Long             | EC               | CORP              | CA        |      8564 | NS      | $167218.02    | 1.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Compagnie de Saint-Gobain SA                 | Compagnie de Saint-Gobain SA EUR4                                     | CUSIP: F80343100<br>LEI: NFONVGN05Z0FMN5PEC35 | Long             | EC               | CORP              | FR        |      3261 | NS      | $355210.52    | 2.53%             |  |  |  | No            |                  2 | On Loan: No      |
| Xylem, Inc.                                  | Xylem, Inc. COM USD0.01                                               | CUSIP: 98419M100<br>LEI: 549300DF5MV96DRYLQ48 | Long             | EC               | CORP              | US        |      1799 | NS      | $265352.50    | 1.89%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2025-11-14

**Name of Applicant:** JANUS ASPEN SERIES

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER