# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0000277751-26-000145
**Filing Date:** 2026-5
**Character Count:** 41740
**Document Hash:** 25413509e7636ef51d9aa241ecc72316
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0000277751-26-000145.hdr.sgml**: 20260529

**ACCESSION NUMBER**: 0000277751-26-000145

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20260331

**FILED AS OF DATE**: 20260529

**DATE AS OF CHANGE**: 20260529

**PERIOD START**: 20260930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 261042066

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Global Life Sciences Fund (Series ID: S000010494)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028956 | Class T      | JAGLX           |
| C000077752 | Class A      | JFNAX           |
| C000077753 | Class C      | JFNCX           |
| C000077754 | Class I      | JFNIX           |
| C000077755 | Class S      | JFNSX           |
| C000083538 | Class D      | JNGLX           |
| C000198286 | Class N      | JFNNX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Global Life Sciences Fund

- **b. EDGAR series identifier (if any):** S000010494

- **c. LEI of Series:** 5493001MPP2BKRJEGV73

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2026-09-30

- **b. Date as of which information is reported:** 2026-03-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $5201271377.30

**Total Liabilities:** $4018914.26

**Net Assets:** $5197252463.04

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028956 | 0.77%                | 2.05%                | -6.41%               |
| Class ID C000077752 | 0.77%                | 2.04%                | -6.41%               |
| Class ID C000077753 | 0.71%                | 1.98%                | -6.47%               |
| Class ID C000077754 | 0.78%                | 2.07%                | -6.40%               |
| Class ID C000077755 | 0.76%                | 2.02%                | -6.43%               |
| Class ID C000083538 | 0.78%                | 2.06%                | -6.40%               |
| Class ID C000198286 | 0.80%                | 2.06%                | -6.39%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $29293831.00             | $13347158.00                               |
| Month 2  | $59028356.00             | $49293080.00                               |
| Month 3  | $15186712.00             | $-377983613.00                             |

**Designated Index Information**

- **Index Name:** MSCI THE WORLD INDEX / Health Care

- **Index Identifier:** MSCI.MC.MXWO0HC

### Schedule of Portfolio Investments

| Name                                          | Title                                                           | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Sonoma Biotherapeutics Inc                    | Sonoma Biotherapeutics Inc - Series B-1                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    669428 | NS      | $525500.98    | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Sonoma Biotherapeutics Inc                    | Sonoma Biotherapeutics Inc - Series B                           | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1255200 | NS      | $985332.00    | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Alnylam Pharmaceuticals Inc.                  | Alnylam Pharmaceuticals Inc. COM USD0.01                        | CUSIP: 02043Q107<br>LEI: 529900S3ZI14OWRJII50 | Long             | EC               | CORP              | US        |     45992 | NS      | $15217373.04  | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Apogee Therapeutics Inc.                      | Apogee Therapeutics Inc. COM                                    | CUSIP: 03770N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    379608 | NS      | $31951605.36  | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| Green Cross Holdings Corp                     | Curevo Inc                                                      | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   2031087 | NS      | $5330344.10   | 0.10%             |  |  |  | Yes           |                  3 | On Loan: No      |
| KARDIUM SERIES 8 CLASS D PREFERRED SHARES USD | Kardium Inc - Series 8 Class D Preferred shares                 | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |  13234820 | NS      | $12237311.62  | 0.24%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Twinstrand Biosciences Inc                    | TwinStrand Biosciences Inc                                      | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    344314 | NS      | $1380526.98   | 0.03%             |  |  |  | Yes           |                  3 | On Loan: No      |
| UCB SA                                        | UCB SA NPV                                                      | CUSIP: B93562120<br>LEI: 2138008J191VLSGY5A09 | Long             | EC               | CORP              | BE        |    104561 | NS      | $31490705.78  | 0.61%             |  |  |  | No            |                  2 | On Loan: No      |
| Sotera Health Company                         | Sotera Health Company COM                                       | CUSIP: 83601L102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1058022 | NS      | $15172035.48  | 0.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Structure Therapeutics Inc                    | Structure Therapeutics Inc SPONSORED ADS                        | CUSIP: 86366E106<br>LEI: N/A                  | Long             |  | CORP              | US        |    569589 | NS      | $27454189.80  | 0.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Hemoshear Therapeutics LLC                    | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    301933 | NS      | $350030.93    | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Green Cross Holdings Corp                     | Curevo Inc - Series B-1                                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    886774 | NS      | $2327231.95   | 0.04%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Tarsus Pharmaceuticals Inc                    | Tarsus Pharmaceuticals Inc COM                                  | CUSIP: 87650L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    296425 | NS      | $20794213.75  | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Teleflex Incorporated                         | Teleflex Incorporated COM USD1                                  | CUSIP: 879369106<br>LEI: UUL85LWXB4NGSWWXIQ25 | Long             | EC               | CORP              | US        |     97944 | NS      | $11715081.84  | 0.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Terns Pharmaceuticals Inc.                    | Terns Pharmaceuticals Inc. COM                                  | CUSIP: 880881107<br>LEI: 254900S1ZDQMF4MYP709 | Long             | EC               | CORP              | US        |    187062 | NS      | $9861908.64   | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Teva Pharmaceutical Industries Ltd.           | Teva Pharmaceutical Industries Ltd. SPON ADS EACH REP 1 ORD SHS | CUSIP: 881624209<br>LEI: 549300BAFGM4RC74ZJ94 | Long             |  | CORP              | US        |   2037481 | NS      | $61368927.72  | 1.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Ardelyx Inc                                   | Ardelyx Inc COM USD0.0001                                       | CUSIP: 039697107<br>LEI: 549300F542QR4SXHCY32 | Long             | EC               | CORP              | US        |   2595821 | NS      | $15548967.79  | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Gilead Sciences Inc.                          | Gilead Sciences Inc. COM USD0.001                               | CUSIP: 375558103<br>LEI: 549300WTZWR07K8MNV44 | Long             | EC               | CORP              | US        |    471681 | NS      | $65738180.97  | 1.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Glaukos Corp.                                 | Glaukos Corp. COM USD0.001                                      | CUSIP: 377322102<br>LEI: 5299004JA1LHE21M6T14 | Long             | EC               | CORP              | US        |    264704 | NS      | $28498032.64  | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                     | Argenx SE SPON ADR EACH REP 1 ORD SHS                           | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    170830 | NS      | $124748607.50 | 2.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                           | Ascendis Pharma A/S SPON ADR EACH REP 1 ORD SHS                 | CUSIP: 04351P101<br>LEI: 549300B66JN3W3J8GH73 | Long             |  | CORP              | US        |    375183 | NS      | $85815607.59  | 1.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Loadsmart Inc                                 | Curevo Inc - Series B                                           | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1348208 | NS      | $3538210.11   | 0.07%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Asher Biotherapeutics Inc                     | Asher Biotherapeutics - Series C                                | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   2580733 | NS      | $809963.05    | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Thermo Fisher Scientific Inc                  | Thermo Fisher Scientific Inc COM USD1                           | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |    265275 | NS      | $130390620.75 | 2.51%             |  |  |  | No            |                  1 | On Loan: No      |
| Travere Therapeutics Inc                      | Travere Therapeutics Inc COM                                    | CUSIP: 89422G107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1432164 | NS      | $42549592.44  | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Globus Med Inc                                | Globus Med Inc COM USD0.001 CL 'A'                              | CUSIP: 379577208<br>LEI: 5299003M2P4CIK0RF205 | Long             | EC               | CORP              | US        |    748604 | NS      | $64499720.64  | 1.24%             |  |  |  | No            |                  1 | On Loan: No      |
| HCA Healthcare Inc                            | HCA Healthcare Inc COM USD0.01                                  | CUSIP: 40412C101<br>LEI: 529900PH4ZGUH2MNEU89 | Long             | EC               | CORP              | US        |    206592 | NS      | $97767598.08  | 1.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Lexeo Therapeutics Inc                        | Lexeo Therapeutics Inc COM                                      | CUSIP: 52886X107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    633486 | NS      | $3636209.64   | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Mckesson Corp.                                | Mckesson Corp. COM USD0.01                                      | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |     79818 | NS      | $69071304.48  | 1.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Merck & Co., Inc                              | Merck & Co., Inc COM USD0.50                                    | CUSIP: 58933Y105<br>LEI: 4YV9Y5M8S0BRK1RP0397 | Long             | EC               | CORP              | US        |    638574 | NS      | $76814066.46  | 1.48%             |  |  |  | No            |                  1 | On Loan: No      |
| HeartFlow Inc.                                | HeartFlow Inc. COM                                              | CUSIP: 42238D107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    585648 | NS      | $14248815.84  | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Humana Inc.                                   | Humana Inc. COM USD0.166                                        | CUSIP: 444859102<br>LEI: 529900YLDW34GJAO4J06 | Long             | EC               | CORP              | US        |     52740 | NS      | $9144588.60   | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| United Therapeutics Corporation               | United Therapeutics Corporation COM USD0.01                     | CUSIP: 91307C102<br>LEI: 5299005C4HZL4UWROC14 | Long             | EC               | CORP              | US        |    116721 | NS      | $69213218.58  | 1.33%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Inc.                       | UnitedHealth Group Inc. COM USD0.01                             | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |    601241 | NS      | $162689802.19 | 3.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                                   | Vaxcyte Inc COM                                                 | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |    971737 | NS      | $56467637.07  | 1.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated           | Vertex Pharmaceuticals Incorporated COM USD0.01                 | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |    239922 | NS      | $107134769.88 | 2.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Xenon Pharmaceuticals Inc                     | Xenon Pharmaceuticals Inc COM NPV                               | CUSIP: 98420N105<br>LEI: 549300XV44Q9Z1MIPQ03 | Long             | EC               | CORP              | US        |    348546 | NS      | $20267949.90  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Zoetis Inc.                                   | Zoetis Inc. COM USD0.01 CL 'A'                                  | CUSIP: 98978V103<br>LEI: 549300HD9Q1LOC9KLJ48 | Long             | EC               | CORP              | US        |    131945 | NS      | $15597218.45  | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEAYA Biosciences Inc.                       | IDEAYA Biosciences Inc. COM USD0.0001                           | CUSIP: 45166A102<br>LEI: 549300ULW08F62IJML11 | Long             | EC               | CORP              | US        |    738748 | NS      | $24615083.36  | 0.47%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc                                  | Illumina Inc COM USD0.01                                        | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |    102214 | NS      | $12598897.64  | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunome Inc                                  | Immunome Inc COM                                                | CUSIP: 45257U108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    368708 | NS      | $8063643.96   | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Insulet Corporation                           | Insulet Corporation COM STK USD0.001                            | CUSIP: 45784P101<br>LEI: 549300TZTYD2PYN92D43 | Long             | EC               | CORP              | US        |     59223 | NS      | $12427354.32  | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                       | Intuitive Surgical Inc. COM USD0.001                            | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |    227319 | NS      | $104791785.81 | 2.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Janux Therapeutics Inc                        | Janux Therapeutics Inc COM                                      | CUSIP: 47103J105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    720074 | NS      | $10009028.60  | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Johnson & Johnson                             | Johnson & Johnson COM USD1.00                                   | CUSIP: 478160104<br>LEI: 549300G0CFPGEF6X2043 | Long             | EC               | CORP              | US        |   1550240 | NS      | $378940665.60 | 7.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc                         | Lantheus Holdings Inc COM USD0.01                               | CUSIP: 516544103<br>LEI: 5299005HUB5IOJGO7S39 | Long             | EC               | CORP              | US        |    518786 | NS      | $39349918.10  | 0.76%             |  |  |  | No            |                  1 | On Loan: No      |
| Janus Henderson Cash Liquidity Fund LLC       | Janus Henderson Cash Liquidity Fund LLC                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        |  20152770 | NS      | $20152769.71  | 0.39%             |  |  |  | No            |                  2 | On Loan: No      |
| Abbott Laboratories                           | Abbott Laboratories COM NPV                                     | CUSIP: 002824100<br>LEI: HQD377W2YR662HK5JX27 | Long             | EC               | CORP              | US        |   1212497 | NS      | $124487066.99 | 2.40%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                   | AbbVie Inc. COM USD0.01                                         | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |    967983 | NS      | $210526622.67 | 4.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Amgen Inc.                                    | Amgen Inc. COM USD0.0001                                        | CUSIP: 031162100<br>LEI: 62QBXGPJ34PQ72Z12S66 | Long             | EC               | CORP              | US        |    270089 | NS      | $95030814.65  | 1.83%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                               | AstraZeneca PLC ORD USD0.25                                     | CUSIP: G0593M107<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |   1221946 | NS      | $238631842.21 | 4.59%             |  |  |  | No            |                  2 | On Loan: No      |
| PFIZER INC                                    | PFIZER INC-CVR                                                  | CUSIP: N/A<br>LEI: N/A                        | Long             |  | CORP              | US        |    390971 | NS      | $1915757.90   | 0.04%             |  |  |  | No            |                  3 | On Loan: No      |
| 4D Molecular Therapeutics Inc                 | 4D Molecular Therapeutics Inc COM                               | CUSIP: 35104E100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    843751 | NS      | $7855321.81   | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Alkermes PLC                                  | ALKERMES PLC-CVR                                                | CUSIP: N/A<br>LEI: N/A                        | Long             |  | CORP              | US        |    450200 | NS      | $288128.00    | 0.01%             |  |  |  | No            |                  3 | On Loan: No      |
| Sanofi SA                                     | Sanofi SA EUR2                                                  | CUSIP: F5548N101<br>LEI: 549300E9PC51EN656011 | Long             | EC               | CORP              | FR        |   1116972 | NS      | $107723985.60 | 2.07%             |  |  |  | No            |                  2 | On Loan: No      |
| Olema Pharmaceuticals Inc.                    | Olema Pharmaceuticals Inc. COM                                  | CUSIP: 68062P106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    669297 | NS      | $9979218.27   | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| PTC Therapeutics, Inc.                        | PTC Therapeutics, Inc. COM USD0.001                             | CUSIP: 69366J200<br>LEI: 549300UJLWOIWFDGB318 | Long             | EC               | CORP              | US        |   1024173 | NS      | $69776906.49  | 1.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Penumbra Inc.                                 | Penumbra Inc. COM USD0.001                                      | CUSIP: 70975L107<br>LEI: 549300RGE06P3ECANU72 | Long             | EC               | CORP              | US        |     73736 | NS      | $24212690.32  | 0.47%             |  |  |  | No            |                  1 | On Loan: No      |
| Praxis Precision Medicines Inc                | Praxis Precision Medicines Inc COM NEW                          | CUSIP: 74006W207<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    394970 | NS      | $127255384.30 | 2.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Scholar Rock Holding Corp.                    | Scholar Rock Holding Corp. COM USD0.001                         | CUSIP: 80706P103<br>LEI: 549300Z1FLPGL4OWJ459 | Long             | EC               | CORP              | US        |    341699 | NS      | $16797922.84  | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Soleno Therapeutics Inc                       | Soleno Therapeutics Inc COM                                     | CUSIP: 834203309<br>LEI: 5493003LCY77N7FXK844 | Long             | EC               | CORP              | US        |    987780 | NS      | $33070874.40  | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Stryker Corporation                           | Stryker Corporation COM USD0.10                                 | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |    260843 | NS      | $85710401.37  | 1.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Protagonist Therapeutics Inc                  | Protagonist Therapeutics Inc COM USD0.00001                     | CUSIP: 74366E102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    160299 | NS      | $16895514.60  | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Regeneron Pharmaceuticals, Inc.               | Regeneron Pharmaceuticals, Inc. COM USD0.001                    | CUSIP: 75886F107<br>LEI: 549300RCBFWIRX3HYQ56 | Long             | EC               | CORP              | US        |     23991 | NS      | $18536406.24  | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc                      | Revolution Medicines Inc COM                                    | CUSIP: 76155X100<br>LEI: 549300JU3X0LBCQ65892 | Long             | EC               | CORP              | US        |   1088018 | NS      | $105809750.50 | 2.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Rhythm Pharmaceuticals Inc                    | Rhythm Pharmaceuticals Inc COM USD0.001                         | CUSIP: 76243J105<br>LEI: 529900JPNWXQ8DPLA551 | Long             | EC               | CORP              | US        |    160804 | NS      | $13985123.88  | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Steris PLC                                    | Steris PLC ORD USD0.001                                         | CUSIP: G8473T100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     81916 | NS      | $18114085.08  | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Roche Holding AG                              | Roche Holding AG CHF0.001                                       | CUSIP: H69293266<br>LEI: 549300U41AUUVOAAOB37 | Long             | EP               | CORP              | CH        |    290904 | NS      | $114896793.77 | 2.21%             |  |  |  | No            |                  2 | On Loan: No      |
| Cencora Inc                                   | Cencora Inc COM USD0.01                                         | CUSIP: 03073E105<br>LEI: AI8GXW8LG5WK7E9UD086 | Long             | EC               | CORP              | US        |    139138 | NS      | $43708811.32  | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Elevance Health Inc.                          | Elevance Health Inc. COM USD0.01                                | CUSIP: 036752103<br>LEI: 8MYN82XMYQH89CTMTH67 | Long             | EC               | CORP              | US        |    165285 | NS      | $48387183.75  | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| Becton, Dickinson and Company                 | Becton, Dickinson and Company COM USD1.00                       | CUSIP: 075887109<br>LEI: ICE2EP6D98PQUILVRZ91 | Long             | EC               | CORP              | US        |    181577 | NS      | $28549351.71  | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation                 | Boston Scientific Corporation COM USD0.01                       | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |   1520117 | NS      | $95387341.75  | 1.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Bridgebio Pharma Inc                          | Bridgebio Pharma Inc COM USD0.001                               | CUSIP: 10806X102<br>LEI: 54930028YG89VTY2J114 | Long             | EC               | CORP              | US        |   1551141 | NS      | $115187730.66 | 2.22%             |  |  |  | No            |                  1 | On Loan: No      |
| CVS Health Corp                               | CVS Health Corp COM USD0.01                                     | CUSIP: 126650100<br>LEI: 549300EJG376EN5NQE29 | Long             | EC               | CORP              | US        |   1071894 | NS      | $76983427.08  | 1.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Centene Corp.                                 | Centene Corp. COM USD0.001                                      | CUSIP: 15135B101<br>LEI: 549300Z7JJ4TQSQGT333 | Long             | EC               | CORP              | US        |    340855 | NS      | $11159592.70  | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Cogent Biosciences Inc.                       | Cogent Biosciences Inc. COM                                     | CUSIP: 19240Q201<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    498576 | NS      | $19190190.24  | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cooper Companies, Inc.                    | The Cooper Companies, Inc. COM                                  | CUSIP: 216648501<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |    281740 | NS      | $20144410.00  | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Crinetics Pharmaceuticals Inc.                | Crinetics Pharmaceuticals Inc. COM USD0.001                     | CUSIP: 22663K107<br>LEI: 549300Q1CNFXK5P31D28 | Long             | EC               | CORP              | US        |    394286 | NS      | $14320467.52  | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                           | Danaher Corporation COM USD0.01                                 | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |    448567 | NS      | $85048303.20  | 1.64%             |  |  |  | No            |                  1 | On Loan: No      |
| DexCom Inc                                    | DexCom Inc COM USD0.001                                         | CUSIP: 252131107<br>LEI: 549300YSK3QDSFR5EU59 | Long             | EC               | CORP              | US        |    561491 | NS      | $35261634.80  | 0.68%             |  |  |  | No            |                  1 | On Loan: No      |
| Dyne Therapeutics Inc.                        | Dyne Therapeutics Inc. COM                                      | CUSIP: 26818M108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2528224 | NS      | $45836701.12  | 0.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Edgewise Therapeutics Inc.                    | Edgewise Therapeutics Inc. COM                                  | CUSIP: 28036F105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    659507 | NS      | $20774470.50  | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Edwards Lifesciences Corporation              | Edwards Lifesciences Corporation COM USD1                       | CUSIP: 28176E108<br>LEI: YA13X31F3V31L8TMPR58 | Long             | EC               | CORP              | US        |    465579 | NS      | $37283566.32  | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Element Biosciences Inc                       | Element Biosciences Inc                                         | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    425023 | NS      | $2461308.19   | 0.05%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Magnolia Medical Technologies                 | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |         1 | NS      | $0.00         | 0.00%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Mirum Pharmaceuticals Inc                     | Mirum Pharmaceuticals Inc                                       | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |    123782 | NS      | $11434981.16  | 0.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Attralus Inc                                  | Attralus Inc                                                    | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    669935 | NS      | $415359.70    | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Novo Nordisk A/S                              | NOVO NORDISK A/S - CVR                                          | CUSIP: N/A<br>LEI: N/A                        | Long             |  | CORP              | US        |    569281 | NS      | $370032.65    | 0.01%             |  |  |  | No            |                  3 | On Loan: No      |
| Freenome Holdings Inc                         | Freenome Holdings Inc - Series D                                | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    342803 | NS      | $1016205.21   | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Magnolia Medical Technologies                 | Magnolia Medical Technologies                                   | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1821717 | NS      | $2568620.97   | 0.05%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Asher Biotherapeutics Inc                     | Asher Biotherapeutics Inc                                       | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |   1214301 | NS      | $381108.37    | 0.01%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Freenome Holdings Inc                         | Freenome Holdings Inc - Series C                                | CUSIP: N/A<br>LEI: N/A                        | Long             | EP               | CORP              | US        |    337474 | NS      | $997910.62    | 0.02%             |  |  |  | Yes           |                  3 | On Loan: No      |
| Eli Lilly & Co.                               | Eli Lilly & Co. COM NPV                                         | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |    499479 | NS      | $459405799.83 | 8.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Madrigal Pharmaceuticals Inc                  | Madrigal Pharmaceuticals Inc USD0.0001                          | CUSIP: 558868105<br>LEI: 549300CHIMT2EUSNJB60 | Long             | EC               | CORP              | US        |    149195 | NS      | $78099106.65  | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Medline Inc.                                  | Medline Inc. COM CL A                                           | CUSIP: 58507V107<br>LEI: 984500FAN6J1P8566D43 | Long             | EC               | CORP              | US        |    864511 | NS      | $38470739.50  | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Mirum Pharmaceuticals Inc.                    | Mirum Pharmaceuticals Inc. COM USD0.0001                        | CUSIP: 604749101<br>LEI: 254900YZKR40F7F27K81 | Long             | EC               | CORP              | US        |    685482 | NS      | $63324827.16  | 1.22%             |  |  |  | No            |                  1 | On Loan: No      |
| Neurocrine Biosciences Inc                    | Neurocrine Biosciences Inc COM USD0.001                         | CUSIP: 64125C109<br>LEI: 549300FECER0XBN49756 | Long             | EC               | CORP              | US        |    136901 | NS      | $18035337.74  | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Novartis AG                                   | Novartis AG ADR-REP 1 CHF0.5 (REGD)                             | CUSIP: 66987V109<br>LEI: 5493007HIVTX6SY6XD66 | Long             |  | CORP              | US        |    971619 | NS      | $148414802.25 | 2.86%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvalent Inc                                  | Nuvalent Inc COM                                                | CUSIP: 670703107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    128313 | NS      | $13145666.85  | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Daiichi Sankyo Co Ltd.                        | Daiichi Sankyo Co Ltd. NPV                                      | CUSIP: J11257102<br>LEI: 353800XGIU2IHQGC9504 | Long             | EC               | CORP              | JP        |   1047000 | NS      | $18460849.35  | 0.36%             |  |  |  | No            |                  2 | On Loan: No      |
| Biohaven Ltd                                  | Biohaven Ltd COM                                                | CUSIP: G1110E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2220238 | NS      | $18783213.48  | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Medtronic PLC                                 | Medtronic PLC USD0.0001                                         | CUSIP: G5960L103<br>LEI: 549300GX3ZBSQWUXY261 | Long             | EC               | CORP              | US        |    583432 | NS      | $50554382.80  | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Euro Member Countries Cash Balance            | Euro Member Countries Cash Balance                              | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             |  | XX        |       287 | OU      | $287.13       | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Japan Yen Cash Balance                        | Japan Yen Cash Balance                                          | CUSIP: N/A<br>LEI: N/A                        | Long             | STIV             |  | JP        |     21344 | OU      | $21344.00     | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2026-05-15

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER