# EDGAR Filing Document

**Accession Number:** 0000277751
**File Stem:** 0001193125-25-301781
**Filing Date:** 2025-11
**Character Count:** 42380
**Document Hash:** dd9860af719f5a0d4bd5717b53cf44ae
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-301781.hdr.sgml**: 20251128

**ACCESSION NUMBER**: 0001193125-25-301781

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20250930

**FILED AS OF DATE**: 20251128

**DATE AS OF CHANGE**: 20251128

**PERIOD START**: 20250930

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JANUS INVESTMENT FUND
- **CENTRAL INDEX KEY:** 0000277751

**ORGANIZATION NAME:**
- **EIN:** 840592523
- **STATE OF INCORPORATION:** MA

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-01879
- **FILM NUMBER:** 251534184

**BUSINESS ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206
- **BUSINESS PHONE:** 303-333-3863

**MAIL ADDRESS:**
- **STREET 1:** 151 DETROIT STREET
- **CITY:** DENVER
- **STATE:** CO
- **ZIP:** 80206

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JANUS FUND /MD/
- **DATE OF NAME CHANGE:** 19870701

## Series and Classes Contracts Data

### Janus Henderson Enterprise Fund (Series ID: S000010490)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000028952 | Class T      | JAENX           |
| C000077737 | Class A      | JDMAX           |
| C000077738 | Class C      | JGRCX           |
| C000077739 | Class I      | JMGRX           |
| C000077740 | Class R      | JDMRX           |
| C000077741 | Class S      | JGRTX           |
| C000083535 | Class D      | JANEX           |
| C000114883 | Class N      | JDMNX           |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** JANUS INVESTMENT FUND

- **b. Investment Company Act file number:** 811-01879

- **c. CIK number of Registrant:** 0000277751

- **d. LEI of Registrant:** W6UYDZFUOFJ4HQQ32382

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 151 Detroit Street

  - **City:** Denver

  - **State:** CO

  - **Foreign country:** US

  - **Zip / Postal Code:** 80206

  - **Telephone number:** 303-333-3863

**Item A.2. Information about the Series.**

- **a. Name of Series:** Janus Henderson Enterprise Fund

- **b. EDGAR series identifier (if any):** S000010490

- **c. LEI of Series:** TJ2Y3QQ1QRI8KQ84FR29

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-09-30

- **b. Date as of which information is reported:** 2025-09-30

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $23624660724.20

**Total Liabilities:** $12507047.71

**Net Assets:** $23612153676.49

**Cash Not Reported:** $4336404.60

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000028952 | 1.41%                | 1.02%                | 0.05%                |
| Class ID C000077737 | 1.39%                | 0.99%                | 0.04%                |
| Class ID C000077738 | 1.33%                | 0.94%                | -0.02%               |
| Class ID C000077739 | 1.41%                | 1.03%                | 0.06%                |
| Class ID C000077740 | 1.35%                | 0.97%                | 0.01%                |
| Class ID C000077741 | 1.38%                | 0.99%                | 0.03%                |
| Class ID C000083535 | 1.41%                | 1.03%                | 0.05%                |
| Class ID C000114883 | 1.42%                | 1.04%                | 0.07%                |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $-6045600.00             | $306361356.00                              |
| Month 2  | $27198186.00             | $225145656.00                              |
| Month 3  | $182649360.00            | $-190897944.00                             |

**Designated Index Information**

- **Index Name:** Russell MidCap Growth

- **Index Identifier:** RUSSELL.MC.RDG

### Schedule of Portfolio Investments

| Name                                        | Title                                                          | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)    | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Descartes Systems Group Inc.                | Descartes Systems Group Inc. COM NPV                           | CUSIP: 249906108<br>LEI: 875500NEPA7JI5EOMN45 | Long             | EC               | CORP              | US        |   1231244 | NS      | $116020122.12  | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Dollar Tree Inc                             | Dollar Tree Inc COM USD0.01                                    | CUSIP: 256746108<br>LEI: 549300PMSTQITB1WHR43 | Long             | EC               | CORP              | US        |   1473986 | NS      | $139100058.82  | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| DoorDash Inc                                | DoorDash Inc CL A                                              | CUSIP: 25809K105<br>LEI: 549300NUQ43FGGSK5051 | Long             | EC               | CORP              | US        |   1353906 | NS      | $368248892.94  | 1.56%             |  |  |  | No            |                  1 | On Loan: No      |
| Dynatrace Inc                               | Dynatrace Inc COM USD0.001                                     | CUSIP: 268150109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2457390 | NS      | $119060545.50  | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson Enterprises Inc                    | Ferguson Enterprises Inc COMMON STOCK NEW                      | CUSIP: 31488V107<br>LEI: 2138003JYQMRP3SLX189 | Long             | EC               | CORP              | US        |   2825411 | NS      | $634530802.38  | 2.69%             |  |  |  | No            |                  1 | On Loan: No      |
| FirstService Corp.                          | FirstService Corp. COM NPV                                     | CUSIP: 33767E202<br>LEI: 5493000XUDIV75BCF118 | Long             | EC               | CORP              | US        |    488535 | NS      | $93061032.15   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Gildan Activewear Inc.                      | Gildan Activewear Inc. COM NPV                                 | CUSIP: 375916103<br>LEI: 549300SB3IBI34M00Z45 | Long             | EC               | CORP              | US        |   5492104 | NS      | $317443611.20  | 1.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Global Payments Inc                         | Global Payments Inc COM NPV                                    | CUSIP: 37940X102<br>LEI: 549300NOMHGVQBX6S778 | Long             | EC               | CORP              | US        |   1491375 | NS      | $123903435.00  | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| Globus Med Inc                              | Globus Med Inc COM USD0.001 CL 'A'                             | CUSIP: 379577208<br>LEI: 5299003M2P4CIK0RF205 | Long             | EC               | CORP              | US        |   2207141 | NS      | $126402965.07  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Godaddy Inc                                 | Godaddy Inc COM USD0.001 CLASS A                               | CUSIP: 380237107<br>LEI: 549300J8H1TB0I2CB447 | Long             | EC               | CORP              | US        |    898387 | NS      | $122926293.21  | 0.52%             |  |  |  | No            |                  1 | On Loan: No      |
| J. B. Hunt Transport Services, Inc.         | J. B. Hunt Transport Services, Inc. COM USD0.01                | CUSIP: 445658107<br>LEI: 549300XCD1MPI1C5GK90 | Long             | EC               | CORP              | US        |   2853222 | NS      | $382816795.74  | 1.62%             |  |  |  | No            |                  1 | On Loan: No      |
| ICU Medical Inc                             | ICU Medical Inc COM USD0.10                                    | CUSIP: 44930G107<br>LEI: 5493003NRW7LS2QZRH60 | Long             | EC               | CORP              | US        |   1230753 | NS      | $147641129.88  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc                                | Illumina Inc COM USD0.01                                       | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |   1338491 | NS      | $127116490.27  | 0.54%             |  |  |  | No            |                  1 | On Loan: No      |
| Ingersoll Rand Inc                          | Ingersoll Rand Inc COM                                         | CUSIP: 45687V106<br>LEI: 5299004C02FMZCUOIR50 | Long             | EC               | CORP              | US        |   4436839 | NS      | $366571638.18  | 1.55%             |  |  |  | No            |                  1 | On Loan: No      |
| KLA Corp.                                   | KLA Corp. COM USD0.001                                         | CUSIP: 482480100<br>LEI: 549300H0BF5JCG96TJ81 | Long             | EC               | CORP              | US        |    236635 | NS      | $255234511.00  | 1.08%             |  |  |  | No            |                  1 | On Loan: No      |
| JANUS CAPITAL GROUP INC                     | Janus Henderson Cash Liquidity Fund LLC                        | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | PF                | US        | 839083192 | NS      | $839251008.41  | 3.55%             |  |  |  | No            |                  2 | On Loan: No      |
| Intact Financial Corp.                      | Intact Financial Corp. COM NPV                                 | CUSIP: 45823T106<br>LEI: 5493009LXGE7Y4U9OY43 | Long             | EC               | CORP              | CA        |   3417236 | NS      | $664955078.49  | 2.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Entain PLC                                  | Entain PLC ORD EUR0.01                                         | CUSIP: G3167C109<br>LEI: 213800GNI3K45LQR8L28 | Long             | EC               | CORP              | GB        |  11134775 | NS      | $130510503.40  | 0.55%             |  |  |  | No            |                  2 | On Loan: No      |
| Flex Ltd.                                   | Flex Ltd. COM USD0.01                                          | CUSIP: Y2573F102<br>LEI: 549300EAQH74YHD07T53 | Long             | EC               | CORP              | US        |  15424119 | NS      | $894136178.43  | 3.79%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $184563.21     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-5139.95      | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-14600.83     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-88925.06     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $708967.84     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $7798.34       | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $23630.88      | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $112627.14     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-199380.29    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| API Group Corp                              | API Group Corp COM STK                                         | CUSIP: 00187Y100<br>LEI: 213800NZT1VX6PZ7BT53 | Long             | EC               | CORP              | US        |  12344160 | NS      | $424268779.20  | 1.80%             |  |  |  | No            |                  1 | On Loan: No      |
| LPL Financial Holdings Inc.                 | LPL Financial Holdings Inc. COM USD0.001                       | CUSIP: 50212V100<br>LEI: 549300XROQ4Y8G2L7682 | Long             | EC               | CORP              | US        |   1522214 | NS      | $506425375.66  | 2.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Lamar Advertising Company                   | Lamar Advertising Company COM USD0.001 CL A                    | CUSIP: 512816109<br>LEI: 529900Y8WW4MKVPTB543 | Long             | EC               | CORP              | US        |   2190671 | NS      | $268181943.82  | 1.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc                       | Lantheus Holdings Inc COM USD0.01                              | CUSIP: 516544103<br>LEI: 5299005HUB5IOJGO7S39 | Long             | EC               | CORP              | US        |   1132936 | NS      | $58108287.44   | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del                      | Liberty Media Corp Del COM LBTY ONE S A                        | CUSIP: 531229771<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |    731603 | NS      | $69663237.66   | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Receive Performance on Equity.RYANAIR HOLDINGS_IE_             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |  18644513 | OU      | $10474195.03   | 0.04%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Receive Performance on Equity.RYANAIR HOLDINGS_IE_             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DE               |  | US        |  26425813 | OU      | $9206709.87    | 0.04%             |  |  |  | No            |                  2 | On Loan: No      |
| Ziff Davis Inc                              | Ziff Davis Inc COM USD0.001                                    | CUSIP: 48123V102<br>LEI: 5493008OJYGLHD7MXA18 | Long             | EC               | CORP              | US        |   1581368 | NS      | $60250120.80   | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| ON Semiconductor Corp.                      | ON Semiconductor Corp. COM USD0.01                             | CUSIP: 682189105<br>LEI: ZV20P4CNJVT8V1ZGJ064 | Long             | EC               | CORP              | US        |   7509031 | NS      | $370270318.61  | 1.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Oneok Inc.                                  | Oneok Inc. COM USD0.01                                         | CUSIP: 682680103<br>LEI: 2T3D6M0JSY48PSZI1Q41 | Long             | EC               | CORP              | US        |   2877348 | NS      | $209960083.56  | 0.89%             |  |  |  | No            |                  1 | On Loan: No      |
| Waters Corporation                          | Waters Corporation COM USD0.01                                 | CUSIP: 941848103<br>LEI: 5T547R1474YC9HOD8Q74 | Long             | EC               | CORP              | US        |    956856 | NS      | $286874997.36  | 1.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Wayfair Inc                                 | Wayfair Inc COM USD0.001 A                                     | CUSIP: 94419L101<br>LEI: 54930027N9ZAVYFDHK53 | Long             | EC               | CORP              | US        |   2173558 | NS      | $194163936.14  | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Workday Inc                                 | Workday Inc COM USD0.001 CL A                                  | CUSIP: 98138H101<br>LEI: 549300J0DYC0N31V7G13 | Long             | EC               | CORP              | US        |    878110 | NS      | $211387420.30  | 0.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Willis Towers Watson Public Limited Company | Willis Towers Watson Public Limited Company COM USD0.000304635 | CUSIP: G96629103<br>LEI: 549300WHC56FF48KL350 | Long             | EC               | CORP              | US        |    641071 | NS      | $221457976.95  | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| NXP Semiconductors NV                       | NXP Semiconductors NV EUR0.20                                  | CUSIP: N6596X109<br>LEI: 724500M9BY5293JDF951 | Long             | EC               | CORP              | US        |   1800147 | NS      | $409947476.31  | 1.74%             |  |  |  | No            |                  1 | On Loan: No      |
| W.R. Berkley Corporation                    | W.R. Berkley Corporation COM USD0.20                           | CUSIP: 084423102<br>LEI: SQOAGCLKBDWNVYV1OV80 | Long             | EC               | CORP              | US        |   5112080 | NS      | $391687569.60  | 1.66%             |  |  |  | No            |                  1 | On Loan: No      |
| PTC Inc                                     | PTC Inc COM USD0.01                                            | CUSIP: 69370C100<br>LEI: 54930050SSMU8SKDJ030 | Long             | EC               | CORP              | US        |   2471133 | NS      | $501689421.66  | 2.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Revvity Inc                                 | Revvity Inc COM USD1.00                                        | CUSIP: 714046109<br>LEI: 549300IKL1SDPFI7N655 | Long             | EC               | CORP              | US        |   5624564 | NS      | $492993034.60  | 2.09%             |  |  |  | No            |                  1 | On Loan: No      |
| RB Global Inc                               | RB Global Inc COM                                              | CUSIP: 74935Q107<br>LEI: 5493002H8U24DJXQ4886 | Long             | EC               | CORP              | US        |   2544231 | NS      | $275692871.16  | 1.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Rentokil Initial PLC                        | Rentokil Initial PLC SPON ADR REP 5 ORD GBP0.01                | CUSIP: 760125104<br>LEI: 549300VN4WV7Z6T14K68 | Long             |  | CORP              | US        |   6389737 | NS      | $161340859.25  | 0.68%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc                    | Revolution Medicines Inc COM                                   | CUSIP: 76155X100<br>LEI: 549300JU3X0LBCQ65892 | Long             | EC               | CORP              | US        |   2086737 | NS      | $97450617.90   | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| Ryanair Holdings PLC                        | Ryanair Holdings PLC ADS EACH REPR 5 ORD EUR0.01               | CUSIP: 783513203<br>LEI: 635400BR2ROC1FVEBQ56 | Long             |  | CORP              | US        |   3441065 | NS      | $207220934.30  | 0.88%             |  |  |  | No            |                  1 | On Loan: No      |
| SS&C Technologies Holdings Inc.             | SS&C Technologies Holdings Inc. COM USD0.01                    | CUSIP: 78467J100<br>LEI: 529900POY8H7NPPNKK71 | Long             | EC               | CORP              | US        |   9770978 | NS      | $867272007.28  | 3.67%             |  |  |  | No            |                  1 | On Loan: No      |
| StandardAero Inc.                           | StandardAero Inc. COM                                          | CUSIP: 85423L103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   5256762 | NS      | $143457034.98  | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| TFI International Inc                       | TFI International Inc COM                                      | CUSIP: 87241L109<br>LEI: 5493007R9W7KO7SJN678 | Long             | EC               | CORP              | US        |   1889400 | NS      | $166380564.00  | 0.70%             |  |  |  | No            |                  1 | On Loan: No      |
| Teledyne Technologies Inc                   | Teledyne Technologies Inc COM USD0.01                          | CUSIP: 879360105<br>LEI: 549300VSMO9KYQWDND94 | Long             | EC               | CORP              | US        |    980451 | NS      | $574583504.04  | 2.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Teleflex Incorporated                       | Teleflex Incorporated COM USD1                                 | CUSIP: 879369106<br>LEI: UUL85LWXB4NGSWWXIQ25 | Long             | EC               | CORP              | US        |   2885870 | NS      | $353115053.20  | 1.50%             |  |  |  | No            |                  1 | On Loan: No      |
| TransUnion                                  | TransUnion COM USD0.01                                         | CUSIP: 89400J107<br>LEI: 549300ZS772LUNUMRB03 | Long             | EC               | CORP              | US        |   2085935 | NS      | $174759634.30  | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| UL Solutions Inc.                           | UL Solutions Inc. CLASS A COM SHS                              | CUSIP: 903731107<br>LEI: 549300F7CUV5ESKV9P71 | Long             | EC               | CORP              | US        |   1411452 | NS      | $100015488.72  | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Vaxcyte Inc                                 | Vaxcyte Inc COM                                                | CUSIP: 92243G108<br>LEI: 254900M7NMHTGASX8D95 | Long             | EC               | CORP              | US        |   2570623 | NS      | $92593840.46   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Veralto Corp.                               | Veralto Corp. COM SHS                                          | CUSIP: 92338C103<br>LEI: 635400FJE6GSOJUSNY27 | Long             | EC               | CORP              | US        |   1744191 | NS      | $185948202.51  | 0.79%             |  |  |  | No            |                  1 | On Loan: No      |
| WEX Inc                                     | WEX Inc COM USD0.01                                            | CUSIP: 96208T104<br>LEI: T9M5IGROL1TL1G5OV478 | Long             | EC               | CORP              | US        |   2499077 | NS      | $393679599.81  | 1.67%             |  |  |  | No            |                  1 | On Loan: No      |
| Rentokil Initial PLC                        | Rentokil Initial PLC ORD GBP0.01                               | CUSIP: G7494G105<br>LEI: 549300VN4WV7Z6T14K68 | Long             | EC               | CORP              | GB        |  16467941 | NS      | $83204109.89   | 0.35%             |  |  |  | No            |                  2 | On Loan: No      |
| Topicus Com Inc                             | Topicus Com Inc SUB VTG SHS                                    | CUSIP: 89072T102<br>LEI: N/A                  | Long             | EC               | CORP              | CA        |   1001381 | NS      | $107406478.08  | 0.45%             |  |  |  | No            |                  1 | On Loan: No      |
| Ryanair Holdings PLC                        | Ryanair Holdings PLC ORD EUR0.006                              | CUSIP: G7727C186<br>LEI: 635400BR2ROC1FVEBQ56 | Long             | EC               | CORP              | IE        |   2031916 | NS      | $59435570.95   | 0.25%             |  |  |  | No            |                  2 | On Loan: No      |
| Sensata Technologies Holding PLC            | Sensata Technologies Holding PLC COM EUR0.01                   | CUSIP: G8060N102<br>LEI: 529900MS7KFYCY9MS037 | Long             | EC               | CORP              | US        |   6977054 | NS      | $213148999.70  | 0.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Steris PLC                                  | Steris PLC ORD USD0.001                                        | CUSIP: G8473T100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |    739181 | NS      | $182902946.64  | 0.77%             |  |  |  | No            |                  1 | On Loan: No      |
| TE Connectivity PLC                         | TE Connectivity PLC ORD SHS                                    | CUSIP: G87052109<br>LEI: 6367003TB6K484NFHE54 | Long             | EC               | CORP              | US        |    801524 | NS      | $175958563.72  | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $5973.70       | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $5134.79       | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $708407.82     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-52890.86     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $542583.03     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-359687.24    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy CAD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-183166.30    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-115188.09    | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-54909.25     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell USD / Buy EUR                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-20751.56     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $139094.55     | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-384.14       | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $1346646.50    | 0.01%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-19427.76     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell CAD / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $1124061.48    | 0.00%             |  |  |  | No            |                  2 | On Loan: No      |
| N/A                                         | Sell EUR / Buy USD                                             | CUSIP: N/A<br>LEI: N/A                        | N/A              | DFE              |  | US        |         1 | NC      | $-33031.05     | -0.00%            |  |  |  | No            |                  2 | On Loan: No      |
| Alliant Energy Corporation                  | Alliant Energy Corporation COM USD0.01                         | CUSIP: 018802108<br>LEI: 5493009ML300G373MZ12 | Long             | EC               | CORP              | US        |   7227173 | NS      | $487183731.93  | 2.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Ameren Corp.                                | Ameren Corp. COM USD0.01                                       | CUSIP: 023608102<br>LEI: XRZQ5S7HYJFPHJ78L959 | Long             | EC               | CORP              | US        |   3512382 | NS      | $366622433.16  | 1.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Applovin Corp.                              | Applovin Corp. COM CL A                                        | CUSIP: 03831W108<br>LEI: 549300LLVXMUAOL3SQ07 | Long             | EC               | CORP              | US        |   1597233 | NS      | $1147675799.82 | 4.86%             |  |  |  | No            |                  1 | On Loan: No      |
| Aramark                                     | Aramark COM USD0.01                                            | CUSIP: 03852U106<br>LEI: 549300MR6PG2DWZUIL39 | Long             | EC               | CORP              | US        |  10081068 | NS      | $387113011.20  | 1.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Argenx SE                                   | Argenx SE SPON ADR EACH REP 1 ORD SHS                          | CUSIP: 04016X101<br>LEI: 7245009C5FZE6G9ODQ71 | Long             |  | CORP              | US        |    328515 | NS      | $242299523.40  | 1.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                         | Ascendis Pharma A/S SPON ADR EACH REP 1 ORD SHS                | CUSIP: 04351P101<br>LEI: 549300B66JN3W3J8GH73 | Long             |  | CORP              | US        |    976876 | NS      | $194212717.56  | 0.82%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.                    | Avidity Biosciences Inc. COM                                   | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   1669830 | NS      | $72754493.10   | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation               | Boston Scientific Corporation COM USD0.01                      | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |   5565902 | NS      | $543399012.26  | 2.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Broadridge Financial Solutions Inc.         | Broadridge Financial Solutions Inc. COM USD0.01                | CUSIP: 11133T103<br>LEI: 549300KZDJZQ2YIHRC28 | Long             | EC               | CORP              | US        |   1784760 | NS      | $425076289.20  | 1.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Burlington Stores Inc.                      | Burlington Stores Inc. COM USD0.0001                           | CUSIP: 122017106<br>LEI: 5299003Z37DVGKKC1W09 | Long             | EC               | CORP              | US        |    546782 | NS      | $139156019.00  | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Cboe Global Markets Inc                     | Cboe Global Markets Inc COM USD0.01                            | CUSIP: 12503M108<br>LEI: 529900RLNSGA90UPEH54 | Long             | EC               | CORP              | US        |    638291 | NS      | $156540867.75  | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| CDW Corp                                    | CDW Corp COM USD0.01                                           | CUSIP: 12514G108<br>LEI: 9845001B052ABF0B6755 | Long             | EC               | CORP              | US        |   1411060 | NS      | $224753636.80  | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| Carmax Inc                                  | Carmax Inc COM USD0.50                                         | CUSIP: 143130102<br>LEI: 529900F60CID3NCZM330 | Long             | EC               | CORP              | US        |   3484996 | NS      | $156371770.52  | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Dayforce Inc                                | Dayforce Inc COM USD0.01                                       | CUSIP: 15677J108<br>LEI: 549300T64GVCHFJ8L449 | Long             | EC               | CORP              | US        |   6506275 | NS      | $448217284.75  | 1.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Clean Harbors, Inc.                         | Clean Harbors, Inc. COM USD0.01                                | CUSIP: 184496107<br>LEI: 5493000SJVZVZJKHJF48 | Long             | EC               | CORP              | US        |   1300549 | NS      | $302013488.78  | 1.28%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cooper Companies, Inc.                  | The Cooper Companies, Inc. COM                                 | CUSIP: 216648501<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |   1678964 | NS      | $115109771.84  | 0.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Corteva Inc                                 | Corteva Inc COM USD0.01                                        | CUSIP: 22052L104<br>LEI: 549300WZN9I2QKLS0O94 | Long             | EC               | CORP              | US        |   4946203 | NS      | $334511708.89  | 1.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Costar Group Inc                            | Costar Group Inc COM USD0.01                                   | CUSIP: 22160N109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   4095740 | NS      | $345557583.80  | 1.46%             |  |  |  | No            |                  1 | On Loan: No      |
| DTE Energy Co.                              | DTE Energy Co. COM NPV                                         | CUSIP: 233331107<br>LEI: 549300IX8SD6XXD71I78 | Long             | EC               | CORP              | US        |   2204308 | NS      | $311755280.44  | 1.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Liberty Media Corp Del                      | Liberty Media Corp Del COM LBTY ONE S C                        | CUSIP: 531229755<br>LEI: 549300ZKUTPIBZLWLL89 | Long             | EC               | CORP              | US        |   4812792 | NS      | $502696124.40  | 2.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Lineage Inc                                 | Lineage Inc COM                                                | CUSIP: 53566V106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |   2693811 | NS      | $104088857.04  | 0.44%             |  |  |  | No            |                  1 | On Loan: No      |
| The Charles Schwab Corporation              | The Charles Schwab Corporation COM USD0.01                     | CUSIP: 808513105<br>LEI: 549300VSGCJ7E698NM85 | Long             | EC               | CORP              | US        |   2185746 | NS      | $208673170.62  | 0.88%             |  |  |  | No            |                  1 | On Loan: No      |
| Constellation Software Inc.                 | Constellation Software Inc. COM STK NPV                        | CUSIP: 21037X100<br>LEI: 549300B6PYHMCTDWQV29 | Long             | EC               | CORP              | CA        |    297017 | NS      | $806413428.43  | 3.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Amdocs Limited                              | Amdocs Limited ORD GBP0.01                                     | CUSIP: G02602103<br>LEI: 549300HQV672RJ7RQV66 | Long             | EC               | CORP              | US        |   1847676 | NS      | $151601815.80  | 0.64%             |  |  |  | No            |                  1 | On Loan: No      |
| Cimpress PLC                                | Cimpress PLC SHS EURO                                          | CUSIP: G2143T103<br>LEI: 549300X1IFD75XISOR81 | Long             | EC               | CORP              | US        |   2355405 | NS      | $148484731.20  | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2025-11-14

**Name of Applicant:** JANUS INVESTMENT FUND

**Signature:** JESPER NERGAARD

**Name of Signer:** JESPER NERGAARD

**Title:** VP, CFO, TREASURER, AND PRINCIPAL ACCOUNTING OFFICER