# EDGAR Filing Document

**Accession Number:** 0001761612
**File Stem:** 0001104659-26-029448
**Filing Date:** 2026-3
**Character Count:** 54991
**Document Hash:** ca8007c0ba47638a42593640b2e98f54
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001104659-26-029448.hdr.sgml**: 20260317

**ACCESSION NUMBER**: 0001104659-26-029448

**CONFORMED SUBMISSION TYPE**: S-8

**PUBLIC DOCUMENT COUNT**: 14

**FILED AS OF DATE**: 20260317

**DATE AS OF CHANGE**: 20260317

**EFFECTIVENESS DATE**: 20260317

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** BICYCLE THERAPEUTICS PLC
- **CENTRAL INDEX KEY:** 0001761612
- **STANDARD INDUSTRIAL CLASSIFICATION:** PHARMACEUTICAL PREPARATIONS [2834]
- **ORGANIZATION NAME:** 03 Life Sciences
- **EIN:** 000000000
- **STATE OF INCORPORATION:** X0
- **FISCAL YEAR END:** 1231

**FILING VALUES:**
- **FORM TYPE:** S-8
- **SEC ACT:** 1933 Act
- **SEC FILE NUMBER:** 333-294374
- **FILM NUMBER:** 26761790

**BUSINESS ADDRESS:**
- **STREET 1:** BLOCKS A & B, PORTWAY BUILDING
- **STREET 2:** GRANTA PARK, GREAT ABINGTON
- **CITY:** CAMBRIDGE
- **STATE:** X0
- **ZIP:** CB21 6GS
- **BUSINESS PHONE:** 011441223261503

**MAIL ADDRESS:**
- **STREET 1:** BLOCKS A & B, PORTWAY BUILDING
- **STREET 2:** GRANTA PARK, GREAT ABINGTON
- **CITY:** CAMBRIDGE
- **STATE:** X0
- **ZIP:** CB21 6GS

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** BICYCLE THERAPEUTICS plc
- **DATE OF NAME CHANGE:** 20190530

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** BICYCLE THERAPEUTICS Ltd
- **DATE OF NAME CHANGE:** 20181212

**As filed with the Securities and Exchange Commission on March 17, 2026.**

**UNITED STATES**

**SECURITIES AND EXCHANGE COMMISSION**

**Washington, D.C. 20549**

**FORM S-8**

**REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933**

**Bicycle Therapeutics plc**

**(Exact name of registrant as specified in its charter)**

---

| | |
|:---|:---|
| **England and Wales**<br> (State or other jurisdiction of<br> incorporation or organization) | **Not applicable**<br> (I.R.S. Employer<br> Identification No.) |
| **Blocks A & B, Portway Building**<br> **Granta Park, Great Abington, Cambridge**<br> **United Kingdom**<br> (Address of Principal Executive Offices) | **CB21 6GS**<br> (Zip Code) |

---

**Amended and Restated Bicycle Therapeutics plc 2020 Equity Incentive Plan**

**Bicycle Therapeutics plc 2019 Employee Share Purchase Plan**

(Full title of the plan)

**Travis Thompson**

**Chief Financial Officer**

**Bicycle Therapeutics Inc.**

**4 Hartwell Place**

**Lexington, MA 02421**

(Name and address of agent for service)

**(617) 945-8155**

(Telephone number, including area code, of agent for service)

***Copy to:***

**Laura Berezin**

**Jaime L. Chase**

**Cooley LLP**

**1700 Seventh Avenue, Suite 1900**

**Seattle, WA 98101**

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company or an emerging growth company. See the definitions of "large accelerated filer," "accelerated filer," "smaller reporting company" and "emerging growth company" in Rule 12b-2 of the Exchange Act.

Large accelerated filer ◻ Accelerated filer ◻ <br> Non-accelerated filer ⌧ Smaller reporting company ⌧ <br> Emerging growth company ◻

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 7(a)(2)(B) of the Securities Act. ◻

**REGISTRATION OF ADDITIONAL SHARES<br> PURSUANT TO GENERAL INSTRUCTION E**

Pursuant to General Instruction E of Form S-8, Bicycle Therapeutics plc (the "Registrant") is filing this Registration Statement with the Securities and Exchange Commission (the "Commission") to register an additional (a) 3,468,394 ordinary shares, nominal value £0.01 per share (the "Ordinary Shares"), of the Registrant under the Amended and Restated Bicycle Therapeutics plc 2020 Equity Incentive Plan (the "2020 Plan"), pursuant to the provisions of the 2020 Plan providing for an automatic increase in the number of shares reserved and available for issuance under the 2020 Plan on January 1, 2026 and (b) 430,000 Ordinary Shares under the Bicycle Therapeutics plc 2019 Employee Share Purchase Plan (the "ESPP"), pursuant to the provisions of the ESPP providing for an automatic increase in the number of shares reserved and available for issuance under the ESPP on January 1, 2026. In accordance with the instructional note to Part I of Form S-8 as promulgated by the Commission, the information specified by Part I of the Form S-8 has been omitted from this Registration Statement.

**PART II<br> INFORMATION REQUIRED IN THE REGISTRATION STATEMENT**

**Item 3. Incorporation of Documents by Reference.**

The following documents filed by the Registrant with the Commission are incorporated herein by reference:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) the contents of the Registrant's Registration Statements on Form S-8 filed with the Commission on [May 23, 2019 (File No. 333-231718)](https://www.sec.gov/Archives/edgar/data/1761612/000110465919031504/a19-10534_1s8.htm), [August 5, 2020 (File No. 333-240993)](https://www.sec.gov/Archives/edgar/data/1761612/000110465920090870/tm2026693-1_s8.htm), [March 11, 2021 (File No. 333-254163)](https://www.sec.gov/Archives/edgar/data/1761612/000110465921035057/tm219375d1_s8.htm), [March 1, 2022 (File No. 333-263156)](https://www.sec.gov/Archives/edgar/data/1761612/000110465922028731/tm227991d1_s8.htm), [February 28, 2023 (File No. 333-270091)](https://www.sec.gov/Archives/edgar/data/1761612/000110465923026705/tm238019d1_s8.htm), [February 20, 2024 (File No. 333-277171)](https://www.sec.gov/Archives/edgar/data/1761612/000110465924025249/tm246505d1_s8.htm), [February 25, 2025 (File No. 333-285197)](https://www.sec.gov/Archives/edgar/data/1761612/000110465925016920/tm257415d1_s8.htm), and [October 30, 2025 (File No. 333-291168)](https://www.sec.gov/Archives/edgar/data/1761612/000110465925104408/tm2529594d1_s8.htm);

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) the Registrant's Annual Report on Form 10-K (File No. 001-38916) for the fiscal year ended December 31, 2025, filed with the Commission on [March 17, 2026](https://www.sec.gov/ix?doc=/Archives/edgar/data/1761612/000110465926028971/bcyc-20251231x10k.htm) (the "Annual Report");

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(c) the Registrant's Current Reports on Form 8-K (File No. 001-38916) filed with the Commission on [January 12, 2026](https://www.sec.gov/ix?doc=/Archives/edgar/data/0001761612/000110465926002706/tm262874d1_8k.htm), [February 3, 2026](https://www.sec.gov/ix?doc=/Archives/edgar/data/0001761612/000110465926009501/tm264844d1_8k.htm) and [March 17, 2026](https://www.sec.gov/ix?doc=/Archives/edgar/data/1761612/000110465926028954/tm268887d1_8k.htm), (in each case, except for information contained therein which is furnished rather than filed); and

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(d) the description of the Registrant's Ordinary Shares and American Depositary Shares contained in [Exhibit 4.6](https://www.sec.gov/Archives/edgar/data/1761612/000155837025001438/bcyc-20241231xex4d6.htm) to the Annual Report on Form 10-K (File No. 001-38916), including any amendments or reports filed for the purpose of updating such description.

All reports and other documents filed by the Registrant after the date hereof pursuant to Sections 13(a), 13(c), 14 and 15(d) of the Securities Exchange Act of 1934, as amended, prior to the filing of a post-effective amendment which indicates that all securities offered hereby have been sold or which deregisters all securities then remaining unsold, shall be deemed to be incorporated by reference herein and to be part hereof from the date of filing of such reports and documents, except for documents or information deemed furnished and not filed in accordance with the rules of the Commission. Any statement contained in a document incorporated or deemed to be incorporated by reference herein shall be deemed to be modified or superseded for purposes of this Registration Statement to the extent that a statement contained herein or in any subsequently filed document that also is deemed to be incorporated by reference herein modifies or supersedes such statement. Any such statement so modified or superseded shall not be deemed, except as so modified or superseded, to constitute a part of this Registration Statement.

**Item 8. Exhibits.**

---

| | |
|:---|:---|
| **Exhibit<br> Number** | **Exhibit Description** |
| [3.1](https://www.sec.gov/Archives/edgar/data/1761612/000155837024010949/bcyc-20240630xex3d1.htm) | [Articles of Association, dated May 16, 2024 (incorporated by reference to Exhibit 3.1 to the Quarterly Report on Form 10-Q (File No. 001-38916) filed with the Commission on August 6, 2024).](https://www.sec.gov/Archives/edgar/data/1761612/000155837024010949/bcyc-20240630xex3d1.htm) |
| [4.1](https://www.sec.gov/Archives/edgar/data/1761612/000104746919003038/a2238712zex-4_1.htm) | [Form of Deposit Agreement (incorporated by reference to Exhibit 4.1 to Amendment No. 1 to the Company's Registration Statement on Form S-1 (File No. 333-231076), filed with the Commission on May 13, 2019).](https://www.sec.gov/Archives/edgar/data/1761612/000104746919003038/a2238712zex-4_1.htm) |
| [4.2](https://www.sec.gov/Archives/edgar/data/1761612/000104746919003038/a2238712zex-4_1.htm) | [Form of American Depositary Receipt (included in Exhibit 4.1) (incorporated by reference to Exhibit 4.2 to Amendment No. 1 to the Company's Registration Statement on Form S-1 (File No. 333-231076), filed with the Commission on May 13, 2019).](https://www.sec.gov/Archives/edgar/data/1761612/000104746919003038/a2238712zex-4_1.htm) |
| [5.1](tm268887d2_ex5-1.htm) | [Opinion of Cooley (UK) LLP.](tm268887d2_ex5-1.htm) |
| [23.1](tm268887d2_ex23-1.htm) | [Consent of PricewaterhouseCoopers LLP.](tm268887d2_ex23-1.htm) |
| [23.2](tm268887d2_ex5-1.htm) | [Consent of Cooley (UK) LLP (included in Exhibit 5.1).](tm268887d2_ex5-1.htm) |
| [24.1](#a_001) | [Power of Attorney (included on the signature page to this Registration Statement).](#a_001) |
| [99.1](https://www.sec.gov/Archives/edgar/data/1761612/000155837023002238/bcyc-20221231xex10d6.htm) | [Amended and Restated Bicycle Therapeutics plc 2020 Equity Incentive Plan (the "2020 Plan") and forms of award thereunder (incorporated by reference to Exhibit 10.6 to the Registrant's Annual Report on Form 10-K (File No. 001-38916), filed with the Commission on February 28, 2023).](https://www.sec.gov/Archives/edgar/data/1761612/000155837023002238/bcyc-20221231xex10d6.htm) |
| [99.2](https://www.sec.gov/Archives/edgar/data/1761612/000155837025010843/bcyc-20250630xex10d3.htm) | [Amendment No. 1 to the 2020 Plan (incorporated by reference to Exhibit 10.3 to the Registrant's Quarterly Report on Form 10-Q (File No. 001-38916), filed with the Commission on August 8, 2025).](https://www.sec.gov/Archives/edgar/data/1761612/000155837025010843/bcyc-20250630xex10d3.htm) |
| [99.3](https://www.sec.gov/Archives/edgar/data/1761612/000104746919003038/a2238712zex-10_5.htm) | [Bicycle Therapeutics plc 2019 Employee Share Purchase Plan (incorporated by reference to Exhibit 10.5 to the Registrant's Registration Statement on Form S-1 (File No. 333-231076), filed with the Commission on May 13, 2019).](https://www.sec.gov/Archives/edgar/data/1761612/000104746919003038/a2238712zex-10_5.htm) |
| [107](tm268887d2_ex-filingfees.htm) | [Filing Fee Table.](tm268887d2_ex-filingfees.htm) |

---

**SIGNATURES**

Pursuant to the requirements of the Securities Act of 1933, the Registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-8 and has duly caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Cambridge, United Kingdom, on March 17, 2026.

---

| | |
|:---|:---|
| **BICYCLE THERAPEUTICS PLC** | **BICYCLE THERAPEUTICS PLC** |
| Name: | /s/ Kevin Lee |
|  | Kevin Lee, Ph.D., MBA |
|  | Chief Executive Officer |

---

**POWER OF ATTORNEY**

We, the undersigned officers and directors of Bicycle Therapeutics plc, hereby severally constitute and appoint Kevin Lee and Travis Thompson, and each of them singly (with full power to each of them to act alone), our true and lawful attorneys-in-fact and agents, with full power of substitution and resubstitution in each of them for him or her and in his or her name, place and stead, and in any and all capacities, to sign any and all amendments (including post-effective amendments) to this Registration Statement on Form S-8 of Bicycle Therapeutics plc and to file the same, with all exhibits thereto and other documents in connection therewith, with the Securities and Exchange Commission, granting unto said attorneys-in-fact and agents, and each of them, full power and authority to do and perform each and every act and thing requisite or necessary to be done in and about the premises, as full to all intents and purposes as he or she might or could do in person, hereby ratifying and confirming all that said attorneys-in-fact and agents or any of them or their or his or her substitute or substitutes may lawfully do or cause to be done by virtue hereof.

Pursuant to the requirements of the Securities Act of 1933, this Registration Statement has been signed below by the following persons in the capacities on March 17, 2026.

---

| | |
|:---|:---|
| **Name** | **Title** |
| /s/ Kevin Lee | Chief Executive Officer and Director |
| Kevin Lee, Ph.D., MBA | (Principal Executive Officer) |
| /s/ Travis Thompson | Chief Financial Officer |
| Travis Thompson | (Principal Financial and Accounting Officer) |
| /s/ Felix J. Baker | Chairman of the Board and Director |
| Felix J. Baker, Ph.D. |  |
| /s/ Janice Bourque | Director |
| Janice Bourque, MBA |  |
| /s/ Roger Dansey | Director |
| Roger Dansey, M.D. |  |
| /s/ Jose-Carlos Gutiérrez-Ramos | Director |
| Jose-Carlos Gutiérrez-Ramos, Ph.D. |  |
| /s/ Hervé Hoppenot | Director |
| Hervé Hoppenot |  |
| /s/ Alessandro Riva | Director |
| Alessandro Riva, MD |  |

---

---

| | |
|:---|:---|
| /s/ Stephen Sands | Director |
| Stephen Sands, MBA |  |
| /s/ Charles Swanton | Director |
| Charles Swanton, MD, PhD |  |
| /s/ Gregory Winter | Director |
| Sir Gregory Winter, FRS |  |
| /s/ Travis Thompson | Authorized Representative in the United States |
| Travis Thompson |  |

---

## Exhibit 5.1

**Exhibit 5.1**

![](tm268887d2_ex5-1img001.jpg)

Claire Keast-Butler

+44 (0) 20 7556 4211

ckeastbutler@cooley.com

Bicycle Therapeutics plc

Blocks A & B, Portway Building

Granta Park, Great Abington

Cambridge CB21 6GS

United Kingdom

17 March 2026

Ladies and Gentlemen:

---

| | |
|:---|:---|
| **Re:** | **Bicycle Therapeutics plc – Registration Statement on Form S-8 – Exhibit 5.1** |

---

**1.** **INTRODUCTION** 

1.1 We have acted as English legal advisers to Bicycle Therapeutics plc, a public limited company incorporated
in England and Wales (the "**Company** "), in relation to the preparation and filing of the registration statement on Form S-8
to which this opinion letter is attached as an exhibit (such registration statement, as amended, including the documents incorporated
by reference therein, the "**Registration Statement**") filed with the United States Securities and Exchange Commission
(the "**SEC**") pursuant to the United States Securities Act of 1933, as amended (the "**Securities Act** ")
and the rules and regulations promulgated thereunder.

1.2 As set out in the Registration Statement, it is proposed that (i) up to 3,468,394 ordinary shares
of the Company each having a nominal value of £0.01 ()"**Ordinary Shares**") will be allotted and issued upon the
exercise or settlement of equity awards granted or to be granted under the Amended and Restated Bicycle Therapeutics plc 2020 Equity Incentive
Plan with Non-Employee Sub-Plan, adopted by the Company's board of directors (the "**Board**" or the "**Directors** ")
on 23 April 2020 and approved by the Company's shareholders on 29 June 2020 and amended and restated by the Board on 14
April 2022 and 4 April 2025 respectively and approved by the Company's shareholders on 27 June 2022 and 17 June 2025
respectively (the "**2020 Equity Incentive Plan**") pursuant to the provisions of the 2020 Equity Incentive Plan providing
for an automatic increase in the number of shares reserved and available for issuance under the 2020 Equity Incentive Plan on 1 January 2026
(the "**2020 Equity Incentive Plan Shares**") and (ii) up to 430,000 Ordinary Shares will be allotted and issued upon
the exercise or settlement of equity awards granted or to be granted under the Bicycle Therapeutics plc 2019 Employee Share Purchase Plan,
adopted by the Board on 9 May 2019 and approved by the Company's shareholders on 13 May 2019 (the "**ESPP** "
and, together with the 2020 Equity Incentive Plan, the "**Plans**") pursuant to the provisions of the ESPP providing for
an automatic increase in the number of shares reserved and available for issuance under the ESPP on 1 January 2026 (the "**ESPP Shares**" and together with the 2020 Equity Incentive Plan Shares, the "**Shares** ").

1.3 We are rendering this letter at the request of the Company in connection with the Registration Statement.
We have taken instructions solely from the Company.

1.4 Except as otherwise defined in this letter, capitalised terms used have the respective meanings given
to them in the Registration Statement (as defined above) and headings are for ease of reference only and shall not affect interpretation.

1.5 All references to legislation in this letter are to the legislation of England unless the contrary is
indicated, and any reference to any provision of any legislation shall include any amendment, modification, re-enactment or extension
thereof, as in force on the date of this letter.

Cooley (UK) LLP 22 Bishopsgate London EC2N 4BQ, UK<br> t: +44 (0) 20 7583 4055 f: +44 (0) 20 7785 9355 cooley.com

Cooley (UK) LLP is a limited liability partnership and is registered in England and Wales with registered number OC395270. Our registered office is at the address above. Cooley (UK) LLP is authorised and regulated by the Solicitors Regulation Authority (SRA number 617791). A list of the members of Cooley (UK) LLP and their professional qualifications is open to inspection at its registered office. The word 'partner,' used in relation to Cooley (UK) LLP, refers to a member of Cooley (UK) LLP or an employee or consultant of Cooley (UK) LLP (or any affiliated firm) of equivalent standing.

Page Two

**2.** **DOCUMENTS** 

For the purpose of issuing this letter, we have reviewed the following documents only:

2.1 a draft PDF copy of the Registration Statement to be filed with the SEC on 17 March 2026;

2.2 a PDF copy of the 2020 Equity Incentive Plan;

2.3 a PDF copy of the ESPP;

2.4 a PDF executed copy of the minutes of a meeting of the Board held on 9 May 2019 (the "**Board Minutes**") at which it was resolved, *inter alia*, to (i) adopt and approve the ESPP effective upon shareholder approval
and (ii) grant the compensation committee of the Board (the "**Compensation Committee**") the authority to administer
the ESPP;

2.5 a PDF executed copy of the written resolutions passed by the shareholders of the Company on 13 May 2019,
in which it was resolved, *inter alia*, to approve the ESPP (the "**Written Shareholder Resolutions** ");

2.6 a PDF executed copy of the written resolutions of the Board adopted on 23 April 2020, in which it
was resolved, *inter alia*, to adopt and approve the 2020 Equity Incentive Plan effective upon shareholder approval and to grant
the Compensation Committee the authority to administer the 2020 Equity Incentive Plan (the "**2020 Written Board Resolutions** ");

2.7 a PDF executed copy of the written resolutions of the Compensation Committee adopted on 29 June 2020,
in which it was resolved, *inter alia*, to delegate certain authorities of the Compensation Committee to the Chief Executive Officer
of the Company to grant certain equity awards (the "**Committee Written Resolutions** ");

2.8 a PDF executed copy of the written resolutions of the Board adopted on 14 April 2022, in which it
was resolved, *inter alia*, to adopt and approve the amendments to the 2020 Equity Incentive Plan effective upon shareholder approval
(the "**2022 Written Board Resolutions** ");

2.9 a PDF executed copy of the written resolutions of the Board adopted on 4 April 2025, in which it
was resolved, *inter alia*, to adopt and approve further amendments to the 2020 Equity Incentive Plan effective upon shareholder
approval (the "**2025 Written Board Resolutions** ", together with the 2020 Written Board Resolutions and the 2022 Written
Board Resolutions, the "**Written Board Resolutions** ");

2.10 a PDF executed copy of the minutes of the annual general meeting of the Company held on 29 June 2020
(the "**2020 AGM**") at which the shareholders of the Company resolved, *inter alia*, to approve the 2020 Equity Incentive
Plan;

2.11 a PDF executed copy of the minutes of the annual general meeting of the Company held on 27 June 2022
(the "**2022 AGM**") at which the shareholders of the Company resolved, *inter alia*, to approve the amendments to
the 2020 Equity Incentive Plan;

2.12 a PDF executed copy of an extract of the resolutions passed at the annual general meeting of the Company
held on 16 May 2024 (the "**2024 AGM**") at which it was resolved, *inter alia*, to (i) authorise the
Board for the purposes of section 551 of the Companies Act 2006 (the "**Companies Act**") to allot shares or to grant rights
to subscribe for, or convert any security into, shares of the Company, up to a maximum aggregate nominal amount of £1,000,000 (the
 "**2024 Allotment Authority** "); and (ii) empower the Board to allot equity securities pursuant to the 2024 Allotment
Authority as if the statutory pre-emption rights contained in section 561(1) of the Companies Act did not apply to such allotment
(the "**2024 AGM Resolutions** ");

Page Three

2.13 a PDF executed copy of the minutes of the annual general meeting of the Company held on 17 June 2025
(the "**2025 AGM**") at which the shareholders of the Company resolved, *inter alia*, to approve the amendments to
the 2020 Equity Incentive Plan;

2.14 a PDF copy of the certificate of incorporation of the Company dated 27 October 2017 and a PDF copy
of the certificate of incorporation on re-registration of the Company as a public company dated 22 May 2019;

2.15 a PDF copy of the articles of association of the Company adopted on 23 May 2019 (the "**Prior Articles** "); and

2.16 a PDF copy of the current articles of association of the Company adopted on 16 May 2024 (the "**Current Articles** ").

**3.** **SEARCHES** 

In addition to examining the documents referred to in paragraph 2 (*Documents*), we have carried out the following searches only:

3.1 an online search at Companies House in England and Wales ()"**Companies House**") with
 respect to the Company, carried out at 9.44 a.m. (London time) on 17 March 2026 (the "**Companies House Search** "); and

3.2 an online enquiry of the Central Registry of Winding-up Petitions at the Insolvency and Companies
 List in England and Wales (the "**Central Registry**") with respect to the Company, carried out at 10.00
 a.m. (London time) on 17 March 2026 (the "**Central Registry Enquiry**" and, together with the Companies
 House Search, the "**Searches** ").

**4.** **OPINION** 

Subject to the assumptions set out in paragraph 5 (*Assumptions*), the scope of the opinion set out in paragraph 6 (*Scope of Opinion*) and the reservations set out in paragraph 7 (*Reservations*), and subject further to the following:

4.1 the Registration Statement, as finally amended, having become effective under the Securities Act;

4.2 the delegations of authority to the Compensation Committee and the Company's Chief Executive Officer,
as applicable, having been validly effected (among other things, in accordance with articles 98 and 97 of the Current Articles, the 2020
Equity Incentive Plan, the ESPP and applicable laws);

4.3 the Directors, the Compensation Committee or the Company's Chief Executive Officer having validly
granted the awards in respect of the Shares under the 2020 Equity Incentive Plan and the ESPP, as applicable;

4.4 the Directors or the Compensation Committee having validly resolved to allot and issue the Shares, or
grant rights to subscribe for the Shares, at duly convened and quorate meetings of the Board or the Compensation Committee, or by way
of duly passed written resolutions of the Board or the Compensation Committee in compliance with all applicable laws and regulations and
with such resolutions being in full force and effect and not having been rescinded or amended;

4.5 the receipt in full of payment for the Shares in an amount of "cash consideration" (as defined
in section 583(3) of the Companies Act) of not less than the aggregate nominal value for such Shares, assuming in each case that
the individual grants or awards under the 2020 Equity Incentive Plan and the ESPP are duly authorised by all necessary corporate action
and duly granted or awarded and exercised in accordance with the requirements of applicable law, the Current Articles, the 2020 Equity
Incentive Plan and the ESPP (and the agreements and awards duly adopted thereunder and in accordance therewith); and

Page Four

4.6 valid entries having been made in relation to the allotment and issue of the Shares in the books and registers
of the Company,

it is our opinion that, as at today's date, the Shares, if and when allotted and issued, registered in the name of the recipient in the register of members of the Company and delivered in accordance with the terms and conditions referred to in the 2020 Equity Incentive Plan and/or the ESPP, as applicable, and as described in the Registration Statement, will be duly and validly authorised and issued, fully paid or credited as fully paid (subject to the receipt of valid consideration by the Company for the issue thereof) and will not be subject to any call for payment of further capital.

**5.** **ASSUMPTIONS** 

In giving the opinion in this letter, we have assumed (without making enquiry or investigation) that:

5.1 all signatures, stamps and seals on all documents are genuine. All original documents are complete, authentic
and up-to-date, and all documents submitted to us as a copy (whether by email or otherwise) are complete and accurate and conform to the
original documents of which they are copies and that no amendments (whether oral, in writing or by conduct of the parties) have been made
to any of the documents since they were examined by us;

5.2 where a document has been examined by us in draft or specimen form, it will be or has been duly executed
in the form of that draft or specimen;

5.3 the Current Articles remain in full force and effect, and no alteration has been made or will be made
to the Current Articles, in each case prior to the relevant date of the granting of rights to subscribe for the Shares and/or the allotment
and issue of the Shares (each such date, an "**Allotment Date** ");

5.4 at the time of each allotment and issue of any Shares the Company shall have received in full "cash
consideration" (as such term is defined in section 583(3) of the Companies Act) equal to the subscription price payable for
such Shares and shall have entered the holder or holders thereof in the register of members of the Company showing that all such Shares
shall have been fully paid up as to their nominal value and any premium thereon as at each Allotment Date;

5.5 each of the Plans has been validly adopted and remains in full force and effect, and no alteration has
been made or will be made to the Plans prior to any Allotment Date;

5.6 in relation to any allotment and issue of any Shares by the Company pursuant to the Plans, the recipient
shall have become entitled to such Shares under the terms of the relevant Plan and such Shares, or rights over Shares, where applicable,
will be fully vested each in accordance with the terms of the relevant Plan and such recipient has or will have complied with all other
requirements of the relevant Plan in connection with the allotment and issue of such Shares;

5.7 all awards have been or will be made under the terms of the 2020 Equity Incentive Plan or the ESPP, as
applicable, that the terms of all awards have not materially deviated or will not materially deviate from the terms set out in the 2020
Equity Incentive Plan or the ESPP, as applicable, and that any Shares will be allotted and issued in accordance with the terms set out
in the 2020 Equity Incentive Plan or the ESPP, as applicable, in accordance with the Current Articles and applicable laws;

5.8 the 2020 Equity Incentive Plan (other than the 2020 Non-Employee Sub-Plan) and the ESPP each qualify as
an "employees' share scheme" as defined in section 1166 of the Companies Act;

5.9 immediately prior to each Allotment Date, the Directors had or shall have sufficient authority and powers
conferred upon them to allot and issue such Shares and grant such rights (as applicable) under section 551 of the Companies Act (unless
such allotment and issue or grant was or is exempt under section 549(2) of the Companies Act) and under section 570 of the Companies
Act as if section 561 of the Companies Act did not apply to such allotment and issue or grant (unless such allotment and issue or grant
was or is exempt from section 561 of the Companies Act pursuant to section 566 of the Companies Act) pursuant to the 2024 AGM Resolutions,
or if the relevant authorities and powers under the 2024 AGM Resolutions have expired or been fully utilised or have otherwise been replaced
the Company in general meeting having duly and validly resolved to grant authorities under section 551 of the Companies Act and powers
under section 570 and/or section 571 of the Companies Act to the Directors to allot and issue the relevant Shares and/or grant rights
to acquire the relevant Shares, and the Directors have not and shall not allot or issue (or purport to allot or issue) Shares and have
not and shall not grant rights (or purport to grant rights) to acquire Shares in excess of such powers or in breach of any other limitation
on their power to allot and issue Shares or grant rights to acquire Shares;

Page Five

5.10 no Shares shall be allotted or issued, or are or shall be committed to be allotted or issued, at a discount
to their nominal value (whether in dollars or equivalent in any other currency);

5.11 all documents, forms and notices which should have been delivered to Companies House in respect of the
Company have been so delivered;

5.12 the information revealed by the Searches is true, accurate, complete and up-to-date in all respects, and
there is no information which should have been disclosed by the Searches that has not been disclosed for any reason and there has been
no alteration in the status or condition of the Company since the date and time that the Searches were made and that the results of the
Searches will remain complete and accurate as at each Allotment Date;

5.13 in relation to the allotment and issue of the Shares, the Directors have acted and will act in the manner
required by section 172 of the Companies Act and the Shares will be allotted and issued in good faith and on bona fide commercial terms
and on arms' length terms and for the purpose of carrying on the business of the Company and that there are reasonable grounds for
believing that the allotment and issue of the Shares will promote the success of the Company for the benefit of its members as a whole;

5.14 there has not been and will not be any bad faith, breach of trust, fraud, coercion, duress or undue influence
on the part of any of the Directors in relation to any allotment and issue of Shares;

5.15 the resolutions set out in the Written Board Resolutions and the Committee Written Resolutions referred
to in paragraph 2 (*Documents*) were validly passed as written resolutions in accordance with the Prior Articles, that all eligible
Directors of the Company (being all the Directors of the Company who would have been entitled to vote on the matter had it been proposed
as a resolution at a Directors' meeting or a meeting of the Compensation Committee, as applicable, but excluding any Director whose
vote is not to be counted in respect of a particular matter) have signed one or more copies of the Written Board Resolutions and/or the
Committee Written Resolutions or will sign one or more copies of the written resolutions of the Board and/or the Compensation Committee
referred to in paragraph 4.4 of this letter, as applicable, that all relevant provisions of the Companies Act and the Prior Articles and/or
the Current Articles, as applicable, were and/or will be complied with and duly observed (including, if applicable, those relating to
the declaration of Directors' interests or the power of interested Directors to vote) and such resolutions were and/or will be duly
adopted, and have not been and will not be revoked or varied and remain in full force and effect and will remain so as at each Allotment
Date;

5.16 the Board Minutes referred to in paragraph 2 (*Documents*) provided to us in connection with the
giving of this opinion, are a true record of the proceedings described therein, and that the meeting recorded in such minutes was and
each meeting of the Directors or the Compensation Committee referred to in paragraph 4.4 of this letter were and/or will be duly conducted
as described therein, duly constituted and convened and all constitutional, statutory and other formalities were and/or will be duly observed
(including, if applicable, those relating to the declaration of Directors' interests or the power of interested Directors to vote),
a quorum was and/or will be present throughout, the requisite majority of Directors voted and/or will vote in favour of approving the
resolutions and the resolutions passed at that meeting of the Board or Compensation Committee, as applicable, were and/or will be duly
adopted, have not been and will not be revoked or varied and remain in full force and effect and will remain so as at each Allotment Date;

Page Six

5.17 the Written Shareholder Resolutions were duly passed as written resolutions on 13 May 2019, the requisite
majority of eligible shareholders signed one or more copies of the written resolutions, all provisions of the Companies Act and the articles
of association of the Company then in effect were duly observed and such resolutions were duly passed and have not been revoked or varied
and remain in full force and effect and will remain so as at each Allotment Date, and all filings required to be made with Companies House
in connection therewith have been made within the relevant time limits;

5.18 the 2020 AGM, 2022 AGM, 2024 AGM and 2025 AGM were duly convened and held on 29 June 2020, 27 June 2022,
16 May 2024 and 17 June 2025 respectively, at which all constitutional, statutory and other formalities were duly observed,
a quorum of shareholders was present throughout and the shareholder resolutions at the 2020 AGM, 2022 AGM, 2024 AGM and 2025 AGM respectively,
were duly passed and have not been revoked or varied and remain in full force and effect and will remain so as at each Allotment Date,
and that all filings required to be made with Companies House in connection therewith have been made within the relevant time limits;

5.19 the resolutions of the shareholders of the Company referred to in paragraph 5.9 will be duly passed as
resolutions of the Company at a duly convened and quorate general meeting of the Company, all constitutional, statutory and other formalities
will be observed in respect of such general meeting and such resolutions will not have expired and will not be revoked or varied prior
to each Allotment Date and will remain in full force and effect as at each Allotment Date;

5.20 the Company has complied and will comply with all applicable anti-terrorism, anti-money laundering, sanctions
and human rights laws and regulations and that each grant of rights to acquire Shares under the 2020 Equity Incentive Plan or the ESPP,
as applicable, and that each allotment and issue of Shares pursuant to the 2020 Equity Incentive Plan or the ESPP, as applicable, will
be consistent with all such laws and regulations;

5.21 no Shares or rights to subscribe for Shares have been or shall be offered to the public in the United
Kingdom in breach of the Financial Services and Markets Act 2000 ()"**FSMA** "), the Public Offers and Admissions to Trading
Regulations 2024, or of any other United Kingdom laws or regulations concerning offers of securities to the public, and no communication
has been or shall be made in relation to the Shares in breach of section 21 (*Restrictions on financial promotion*) of FSMA or any
other United Kingdom laws or regulations relating to offers or invitations to subscribe for, or to acquire rights to subscribe for or
otherwise acquire, shares or other securities;

5.22 in issuing and allotting and granting rights to acquire Shares and administering the 2020 Equity Incentive
Plan and the ESPP, the Company is not carrying on a regulated activity (within the meaning of section 19 (*The general prohibition*)
of FSMA); and

5.23 the Company has not taken any corporate or other action nor have any steps been taken or legal proceedings
been started against the Company for the liquidation, winding-up, dissolution or reorganisation of, or for the appointment of a liquidator,
receiver, trustee, administrator, administrative receiver or similar officer of, any such party (including the Company) or all or any
of its or their assets (or any analogous proceedings in any jurisdiction) and no such steps or proceedings will have been taken as at
each Allotment Date, and the Company is not unable to pay its debts as they fall due within the meaning of section 123 of the Insolvency
Act 1986, as amended (the "**Insolvency Act**") and will not become unable to pay its debts within the meaning of that
section as a result of any of the transactions contemplated herein, is not insolvent and has not been dissolved (although the Searches
gave no indication that any winding-up, dissolution or administration order or appointment of a receiver, administrator, administrative
receiver or similar officer has been made with respect to the Company) and such actions and steps will not have been taken as at any Allotment
Date.

Page Seven

**6.** **SCOPE OF OPINION** 

6.1 The opinion given in this letter is limited to English law as it would be applied by English courts on
the date of this letter.

6.2 We express no opinion in this letter on the laws of any other jurisdiction. We have not investigated the
laws of any country other than England and we assume that no foreign law affects the opinion stated in paragraph 4 (*Opinion*).

6.3 We express no opinion as to any agreement, instrument or other document other than as specified in this
letter. For the purposes of giving the opinion in paragraph 4 (*Opinion*), we have only examined and relied on those documents set
out in paragraph 2 (*Documents*) and made those searches and enquiries set out in paragraph 3 (*Searches*), respectively. We
have made no further enquiries concerning the Company or any other matter in connection with the giving of the opinion in paragraph 4
(*Opinion*).

6.4 No opinion is expressed with respect to taxation in the United Kingdom or otherwise in this letter.

6.5 We have not been responsible for investigating or verifying the accuracy of the facts or the reasonableness
of any statement of opinion or intention, contained in or relevant to any document referred to in this letter, or that no material facts
have been omitted therefrom.

6.6 The opinion given in this letter is given on the basis of each of the assumptions set out in paragraph
5 (*Assumptions*) and is subject to each of the reservations set out in paragraph 7 (*Reservations*) to this letter. The opinion
given in this letter is strictly limited to the matters stated in paragraph 4 (*Opinion*) and does not extend, and should not be
read as extending, by implication or otherwise, to any other matters.

6.7 This letter only applies to those facts and circumstances which exist as at today's date and we
assume no obligation or responsibility to update or supplement this letter to reflect any facts or circumstances which may subsequently
come to our attention, any changes in laws which may occur after today, or to inform the addressee of any change in circumstances happening
after the date of this letter which would alter the opinion given in this letter.

6.8 We have not been responsible for investigation or verification of statements of fact (including statements
as to foreign law) or to the reasonableness of any statements of opinion in the Registration Statement, or that no material facts have
been omitted therefrom.

6.9 This letter is given by Cooley (UK) LLP and no partner or employee assumes any personal responsibility
for it nor shall owe any duty of care in respect of it.

6.10 This letter, the opinion given in it, and any non-contractual obligations arising out of or in connection
with this letter and/or the opinion given in it, are governed by and shall be construed in accordance with English law as at the date
of this letter.

**7.** **RESERVATIONS** 

7.1 The Companies House Search described at paragraph 3.1 (*Searches*) is not capable of revealing conclusively
whether or not:

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(a) a winding-up order has been made or a resolution passed for the winding-up of a company;

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(b) an administration order has been made; or

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(c) a receiver, administrative receiver, administrator or liquidator has been appointed,

since notice of these matters may not be filed with the Registrar of Companies in England and Wales immediately and, when filed, may not be entered on the public database or recorded on the public microfiches of the relevant company immediately.

Page Eight

In addition, such a company search is not capable of revealing, prior to the making of the relevant order, whether or not a winding-up petition or a petition for an administration order has been presented.

7.2 The Central Registry Enquiry described at paragraph 3.2 (*Searches*) relates only to a compulsory
winding-up and is not capable of revealing conclusively whether or not a winding-up petition in respect of a compulsory winding-up has
been presented, since details of the petition may not have been entered on the records of the Central Registry immediately or, in the
case of a petition presented to a District Registry and/or County Court in England and Wales, may not have been notified to the Central
Registry and entered on such records at all, and the response to an enquiry only relates to the period since approximately 2016 for petitions
presented in London and since approximately 2019 for petitions presented to a District Registry and/or County Court in England and Wales.
We have not made enquiries of any District Registry or County Court in England and Wales.

7.3 The opinion set out in this letter is subject to: (i) any limitations
arising from applicable laws relating to insolvency, bankruptcy, administration, reorganisation, liquidation, moratoria, schemes or analogous
circumstances; and (ii) an English court exercising its discretion under section 426 of the Insolvency Act (*co-operation between courts exercising jurisdiction in relation to insolvency*) to assist the courts having the corresponding jurisdiction in any part of
the United Kingdom or any relevant country or territory.

7.4 We express no opinion as to matters of fact.

7.5 We have made no enquiries of any individual connected with the Company.

7.6 We express no opinion on the compliance of the 2020 Equity Incentive Plan or the ESPP, or the compliance
of any award made or to be made under the 2020 Equity Incentive Plan or the ESPP, with the rules or regulations of the Nasdaq Global
Select Market or the rules or regulations of any other securities exchange that are applicable to the Company.

7.7 A certificate, documentation, notification, opinion or the like might be held by the English courts not
to be conclusive if it can be shown to have an unreasonable or arbitrary basis or in the event of a manifest error.

7.8 We express no opinion in relation to the legality, enforceability or validity of the 2020 Equity Incentive
Plan or the ESPP or any award agreement entered into or to be entered into pursuant to the 2020 Equity Incentive Plan or the ESPP. In
particular, but without prejudice to the generality of the foregoing, we have assumed that the Shares to be allotted under the 2020 Equity
Incentive Plan or the ESPP, as applicable, or any such award agreement will be paid up in full (as to their nominal value and any premium)
in cash (within the meaning of section 583(1) of the Companies Act) and we express no opinion as to whether any consideration other
than "cash consideration" (as such term is defined in section 583(3) of the Companies Act) which might be paid, or purport
to be paid, for the Shares would result in such Shares being validly issued, fully paid and not subject to any call for payment of further
capital.

7.9 If (a) the Company or a person to whom the Shares are to be allotted and issued (a "**Relevant Person**") is the target of economic or financial sanctions or other restrictive measures imposed in any jurisdiction ()"**Sanctions** ")
or is owned or controlled (directly or indirectly) by or is acting on behalf of or at the direction of or is otherwise connected with
a person who is a target of Sanctions or (b) a Relevant Person is incorporated or resident in or operating from a country or territory
that is a target of Sanctions or (c) the rights or obligations of a Relevant Person is otherwise affected by Sanctions, then the
rights and obligations of such Relevant Person under the 2020 Equity Incentive Plan or the ESPP may be void and/or unenforceable.

7.10 We express no opinion in this letter on the application or potential application of the National Security
and Investment Act 2021 in relation to the 2020 Equity Incentive Plan, the ESPP or any transaction contemplated thereby.

Page Nine

**8.** **DISCLOSURE AND RELIANCE** 

8.1 This letter is addressed to you solely for your benefit in connection with the Registration Statement.
We consent to the filing of this letter as an exhibit to the Registration Statement. In giving such consent, we do not thereby admit that
we are in the category of persons whose consent is required under section 7 of the Securities Act or the rules and regulations promulgated
thereunder.

8.2 This letter may not be relied upon by you for any other purpose, or furnished to, assigned to, quoted
to, or relied upon by any other person, firm or other entity for any purpose, other than for the purpose set out in above in paragraph
8.1, without our prior written consent, which may be granted or withheld at our sole discretion.

Yours faithfully

/s/ Cooley (UK) LLP

**Cooley (UK) LLP**

## Exhibit 23.1

**Exhibit 23.1**

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in this Registration Statement on Form S-8 of Bicycle Therapeutics plc of our report dated March 17, 2026, relating to the financial statements, which appears in Bicycle Therapeutics plc's Annual Report on Form 10-K for the year ended December 31, 2025.

/s/ PricewaterhouseCoopers LLP<br> Cambridge, United Kingdom ****<br> March 17, 2026

## Ex-Filing

?xml version='1.0' encoding='ASCII'? EX-FILING FEES

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| |
|:---|
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Calculation of Filing Fee Tables**  |
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **S-8**  |
| &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **BICYCLE THERAPEUTICS PLC**  |

---

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| | | | | | | | | |
|:---|:---|:---|:---|:---|:---|:---|:---|:---|
| | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Security Type**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Security Class Title**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Fee Calculation Rule**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Amount Registered**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Proposed Maximum Offering Price Per Unit**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Maximum Aggregate Offering Price**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Fee Rate**  | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Amount of Registration Fee**  |
| 1 | Equity | Ordinary shares, nominal value GBP0.01 per share | Other | 3468394 | $4.935 | $17116524.39 | 0.0001381 | $2363.79 |
| 2 | Equity | Ordinary shares, nominal value GBP0.01 per share | Other | 430000 | $4.195 | $1803850.00 | 0.0001381 | $249.11 |
| Total Offering Amounts: | Total Offering Amounts: | Total Offering Amounts: | Total Offering Amounts: | Total Offering Amounts: |  | $18920374.39  |  | $2612.90  |
| Total Fee Offsets:  | Total Fee Offsets:  | Total Fee Offsets:  | Total Fee Offsets:  | Total Fee Offsets:  |  |  |  | $0.00  |
| Net Fee Due:  | Net Fee Due:  | Net Fee Due:  | Net Fee Due:  | Net Fee Due:  |  |  |  | $2612.90  |

---

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; **Offering Note** <br>

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <sup>1</sup> These shares may be represented by the American Depositary Shares ("ADSs") of Bicycle Therapeutics plc (the "Registrant"). Each ADS represents one ordinary share, nominal value GBP0.01 per share (an "Ordinary Share"). The "Amount Registered" represents Ordinary Shares of the Registrant that became available for issuance under the Amended and Restated Bicycle Therapeutics plc 2020 Equity Incentive Plan (the "2020 Plan"), effective as of January 1, 2026, pursuant to the evergreen increase provision of the 2020 Plan. Pursuant to Rule 416 under the Securities Act of 1933, as amended (the "Securities Act"), this Registration Statement shall also cover any additional Ordinary Shares of the Registrant which become issuable under the 2020 Plan by reason of any stock dividend, stock split, recapitalization or any other similar transaction effected without the receipt of consideration which results in an increase in the number of the Registrant's outstanding Ordinary Shares. "Proposed Maximum Offering Price Per Share" and "Maximum Aggregate Offering Price" are estimated solely for the purpose of determining the registration fee pursuant to Rules 457(c) and (h) under the Securities Act, based upon the average of the high and low prices of the Registrant's ADSs as quoted on the Nasdaq Global Select Market on March 13, 2026.

&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <sup>2</sup> These shares may be represented by the ADSs of the Registrant. Each ADS represents one Ordinary Share. The "Amount Registered" represents Ordinary Shares that became available for issuance under the Bicycle Therapeutics plc 2019 Employee Share Purchase Plan (the "ESPP"), effective as of January 1, 2026, pursuant to the evergreen increase provision of the ESPP. Pursuant to Rule 416 under the Securities Act, this Registration Statement shall also cover any additional Ordinary Shares of the Registrant which become issuable under the ESPP by reason of any stock dividend, stock split, recapitalization or any other similar transaction effected without the receipt of consideration which results in an increase in the number of the Registrant's outstanding Ordinary Shares. "Proposed Maximum Offering Price Per Share" and "Maximum Aggregate Offering Price" are estimated solely for the purpose of calculating the registration fee pursuant to Rules 457(c) and 457(h) under the Securities Act and are based on 85% of the average of the high and low sale prices of the Registrant's ADSs as reported on the Nasdaq Global Select Market on March 13, 2026. Pursuant to the ESPP, the purchase price of the Ordinary Shares reserved for issuance thereunder will be 85% of the fair market value of an Ordinary Share on the first trading day of the offering period or on the exercise date, whichever is lower.

---

| |
|:---|
| |
| **Rule 457(p)** |
| Fee Offset Claims |
| Fee Offset Sources |

---