# EDGAR Filing Document

**Accession Number:** 0000756913
**File Stem:** 0001145549-23-009568
**Filing Date:** 2023-2
**Character Count:** 76223
**Document Hash:** 01a4d3c243814fe5b6fce905b66e1d0f
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001145549-23-009568.hdr.sgml**: 20230224

**ACCESSION NUMBER**: 0001145549-23-009568

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 1

**CONFORMED PERIOD OF REPORT**: 20221231

**FILED AS OF DATE**: 20230224

**DATE AS OF CHANGE**: 20230224

**PERIOD START**: 20221231

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JOHN HANCOCK VARIABLE INSURANCE TRUST
- **CENTRAL INDEX KEY:** 0000756913
- **IRS NUMBER:** 000000000
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 0630

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-04146
- **FILM NUMBER:** 23664247

**BUSINESS ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116
- **BUSINESS PHONE:** 617-663-3000

**MAIL ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** JOHN HANCOCK TRUST
- **DATE OF NAME CHANGE:** 20050124

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** MANUFACTURERS INVESTMENT TRUST
- **DATE OF NAME CHANGE:** 19971022

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** NASL SERIES FUND INC
- **DATE OF NAME CHANGE:** 19881030

## Series and Classes Contracts Data

### Health Sciences Trust (Series ID: S000008239)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000022442 | NAV          |  |
| C000022443 | Series I     | JEHSX           |
| C000022444 | Series II    |  |

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** John Hancock Variable Insurance Trust

- **b. Investment Company Act file number:** 811-04146

- **c. CIK number of Registrant:** 0000756913

- **d. LEI of Registrant:** Z5DUWKEPW78QKD87GA06

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 200 Berkeley Street

  - **City:** Boston

  - **State:** MA

  - **Foreign country:** US

  - **Zip / Postal Code:** 02116

  - **Telephone number:** 617-312-0176

**Item A.2. Information about the Series.**

- **a. Name of Series:** Health Sciences Trust

- **b. EDGAR series identifier (if any):** S000008239

- **c. LEI of Series:** K5HWWGQWUOYDF5BZA919

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2022-12-31

- **b. Date as of which information is reported:** 2022-12-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $278368363.33

**Total Liabilities:** $394339.47

**Net Assets:** $277974023.86

**Delayed Delivery Securities:** $173362.41

**Cash Not Reported:** $32325.18

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000022442 | 7.95%                | 4.31%                | -2.86%               |
| Class ID C000022443 | 7.93%                | 4.33%                | -2.89%               |
| Class ID C000022444 | 7.96%                | 4.30%                | -2.90%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $847764.86               | $19759297.74                               |
| Month 2  | $2507722.94              | $9495385.53                                |
| Month 3  | $1093668.45              | $-9378078.55                               |

### Schedule of Portfolio Investments

| Name                                              | Title                                                  | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| Immunocore Holdings PLC                           | IMMUNOCORE HOLDINGS PLC ADR GBP.0001                   | CUSIP: 45258D105<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |     19425 | NS      | $1108584.75   | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Immunovant Inc.                                   | IMMUNOVANT INC COMMON STOCK USD.0001                   | CUSIP: 45258J102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3600 | NS      | $63900.00     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Inari Medical Inc.                                | INARI MEDICAL INC COMMON STOCK USD.001                 | CUSIP: 45332Y109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7791 | NS      | $495195.96    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Elanco Animal Health Incorporated                 | ELANCO ANIMAL HEALTH INC COMMON STOCK                  | CUSIP: 28414H103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17093 | NS      | $208876.46    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Enanta Pharmaceuticals Inc.                       | ENANTA PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 29251M106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3200 | NS      | $148864.00    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Entrada Therapeutics Inc.                         | ENTRADA THERAPEUTICS INC COMMON STOCK USD.0001         | CUSIP: 29384C108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11625 | NS      | $157170.00    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Exact Sciences Corporation                        | EXACT SCIENCES CORP COMMON STOCK USD.01                | CUSIP: 30063P105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20307 | NS      | $1005399.57   | 0.36%             |  |  |  | No            |                  1 | On Loan: No      |
| Exelixis Inc.                                     | EXELIXIS INC COMMON STOCK USD.001                      | CUSIP: 30161Q104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     42625 | NS      | $683705.00    | 0.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Exscientia plc                                    | EXSCIENTIA PLC ADR                                     | CUSIP: 30223G102<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |      4147 | NS      | $22103.51     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| F-star Therapeutics Inc.                          | F-STAR THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 30315R107<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |      1270 | NS      | $8026.40      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Fate Therapeutics Inc.                            | FATE THERAPEUTICS INC COMMON STOCK USD.001             | CUSIP: 31189P102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21179 | NS      | $213696.11    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| FibroGen Inc.                                     | FIBROGEN INC COMMON STOCK USD.01                       | CUSIP: 31572Q808<br>LEI: 549300Q914ULWWY95822 | Long             | EC               | CORP              | US        |      7100 | NS      | $113742.00    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| GE HealthCare Technologies Inc.                   | GE HEALTHCARE TECHNOLOGIES INC COMMON STOCK            | CUSIP: 36266G107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3016 | NS      | $176074.08    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Galapagos NV                                      | GALAPAGOS NV ADR                                       | CUSIP: 36315X101<br>LEI: 549300QKJ78IY0IOV655 | Long             | EC               | CORP              | BE        |      1700 | NS      | $75446.00     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Generation Bio Co.                                | GENERATION BIO CO COMMON STOCK USD.0001                | CUSIP: 37148K100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     37692 | NS      | $148129.56    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Ginkgo Bioworks Holdings Inc.                     | SOARING EAGLE ACQUISITION CO COMMON STOCK USD.0001     | CUSIP: 37611X100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     78858 | NS      | $133270.02    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Gossamer Bio Inc.                                 | GOSSAMER BIO INC COMMON STOCK USD.0001                 | CUSIP: 38341P102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9700 | NS      | $21049.00     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Guardant Health Inc.                              | GUARDANT HEALTH INC COMMON STOCK USD.00001             | CUSIP: 40131M109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18660 | NS      | $507552.00    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| HCA Healthcare Inc.                               | HCA HEALTHCARE INC COMMON STOCK USD.01                 | CUSIP: 40412C101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10763 | NS      | $2582689.48   | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| Hologic Inc.                                      | HOLOGIC INC COMMON STOCK USD.01                        | CUSIP: 436440101<br>LEI: 549300DYP6F5ZJL0LB74 | Long             | EC               | CORP              | US        |     35091 | NS      | $2625157.71   | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Humana Inc.                                       | HUMANA INC COMMON STOCK USD.166                        | CUSIP: 444859102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12192 | NS      | $6244620.48   | 2.25%             |  |  |  | No            |                  1 | On Loan: No      |
| IGM Biosciences Inc.                              | IGM BIOSCIENCES INC COMMON STOCK USD.01                | CUSIP: 449585108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8499 | NS      | $144567.99    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| iRhythm Technologies Inc.                         | IRHYTHM TECHNOLOGIES INC COMMON STOCK USD.001          | CUSIP: 450056106<br>LEI: 549300FJI72RJGDQS384 | Long             | EC               | CORP              | US        |      4478 | NS      | $419454.26    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| IDEAYA Biosciences Inc.                           | IDEAYA BIOSCIENCES INC COMMON STOCK                    | CUSIP: 45166A102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1864 | NS      | $33868.88     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Illumina Inc.                                     | ILLUMINA INC COMMON STOCK USD.01                       | CUSIP: 452327109<br>LEI: SQ95QG8SR5Q56LSNF682 | Long             | EC               | CORP              | US        |      6331 | NS      | $1280128.20   | 0.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Immuneering Corporation                           | IMMUNEERING CORP COMMON STOCK USD.001                  | CUSIP: 45254E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     29676 | NS      | $143928.60    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| QuidelOrtho Corporation                           | QUIDELORTHO CORP COMMON STOCK USD.001                  | CUSIP: 219798105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7801 | NS      | $668311.67    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Cytokinetics Incorporated                         | CYTOKINETICS INC COMMON STOCK USD.001                  | CUSIP: 23282W605<br>LEI: 549300BE5DY1HW7IHA64 | Long             | EC               | CORP              | US        |      9236 | NS      | $423193.52    | 0.15%             |  |  |  | No            |                  1 | On Loan: No      |
| DICE Therapeutics Inc.                            | DICE THERAPEUTICS INC COMMON STOCK USD.0001            | CUSIP: 23345J104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13895 | NS      | $433524.00    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Danaher Corporation                               | DANAHER CORP COMMON STOCK USD.01                       | CUSIP: 235851102<br>LEI: S4BKK9OTCEWQ3YHPFM11 | Long             | EC               | CORP              | US        |     39935 | NS      | $10599547.70  | 3.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Day One Biopharmaceuticals Inc.                   | DAY ONE BIOPHARMACEUTICALS INC COMMON STOCK USD.0001   | CUSIP: 23954D109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15041 | NS      | $323682.32    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Deciphera Pharmaceuticals Inc.                    | DECIPHERA PHARMACEUTICALS INC COMMON STOCK USD.01      | CUSIP: 24344T101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9500 | NS      | $155705.00    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Denali Therapeutics Inc.                          | DENALI THERAPEUTICS INC COMMON STOCK USD.01            | CUSIP: 24823R105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21163 | NS      | $588543.03    | 0.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Dexcom Inc.                                       | DEXCOM INC COMMON STOCK USD.001                        | CUSIP: 252131107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20556 | NS      | $2327761.44   | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Doximity Inc.                                     | DOXIMITY INC COMMON STOCK USD.001                      | CUSIP: 26622P107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     25393 | NS      | $852189.08    | 0.31%             |  |  |  | No            |                  1 | On Loan: No      |
| EQRx Inc.                                         | EQRX INC COMMON STOCK                                  | CUSIP: 26886C107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     41735 | NS      | $102668.10    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| EQRx Inc.                                         | EQRX INC WTS CALL EXP09APR28                           | CUSIP: 26886C115<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4502 | NS      | $1206.09      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Lantheus Holdings Inc.                            | LANTHEUS HOLDINGS INC COMMON STOCK USD.01              | CUSIP: 516544103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4886 | NS      | $248990.56    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Legend Biotech Corporation                        | LEGEND BIOTECH CORP ADR                                | CUSIP: 52490G102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10352 | NS      | $516771.84    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| LIANBIO ADR USD.0001                              | LIANBIO ADR USD.0001                                   | CUSIP: 53000N108<br>LEI: N/A                  | Long             | EC               | CORP              | KY        |     38982 | NS      | $63930.48     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Eli Lilly and Company                             | ELI LILLY and CO COMMON STOCK                          | CUSIP: 532457108<br>LEI: FRDRIPF3EKNDJ2CQJL29 | Long             | EC               | CORP              | US        |     34128 | NS      | $12485387.52  | 4.49%             |  |  |  | No            |                  1 | On Loan: No      |
| Longboard Pharmaceuticals Inc.                    | LONGBOARD PHARMACEUTICALS INC COMMON STOCK USD.0001    | CUSIP: 54300N103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      6452 | NS      | $20388.32     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Surgery Partners Inc.                             | SURGERY PARTNERS INC COMMON STOCK USD.01               | CUSIP: 86881A100<br>LEI: 549300LC82HG33S19I03 | Long             | EC               | CORP              | US        |     10676 | NS      | $297433.36    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Teleflex Incorporated                             | TELEFLEX INC COMMON STOCK USD1.0                       | CUSIP: 879369106<br>LEI: UUL85LWXB4NGSWWXIQ25 | Long             | EC               | CORP              | US        |      5890 | NS      | $1470320.70   | 0.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Tenaya Therapeutics Inc.                          | TENAYA THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 87990A106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     24566 | NS      | $49377.66     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| 10x Genomics Inc.                                 | 10X GENOMICS INC COMMON STOCK                          | CUSIP: 88025U109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     25215 | NS      | $918834.60    | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Thermo Fisher Scientific Inc.                     | THERMO FISHER SCIENTIFIC INC COMMON STOCK USD1.0       | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |     25786 | NS      | $14200092.34  | 5.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Theseus Pharmaceuticals Inc.                      | THESEUS PHARMACEUTICALS INC COMMON STOCK USD.0001      | CUSIP: 88369M101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20251 | NS      | $100849.98    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Third Harmonic Bio Inc.                           | THIRD HARMONIC BIO INC COMMON STOCK USD.0001           | CUSIP: 88427A107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      6379 | NS      | $27429.70     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Twist Bioscience Corporation                      | TWIST BIOSCIENCE CORP COMMON STOCK USD.00001           | CUSIP: 90184D100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8130 | NS      | $193575.30    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Ultragenyx Pharmaceutical Inc.                    | ULTRAGENYX PHARMACEUTICAL INC COMMON STOCK USD.001     | CUSIP: 90400D108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     24815 | NS      | $1149678.95   | 0.41%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Incorporated                   | UNITEDHEALTH GROUP INC COMMON STOCK USD.01             | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |     45191 | NS      | $23959364.38  | 8.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Veeva Systems Inc.                                | VEEVA SYSTEMS INC COMMON STOCK USD.00001               | CUSIP: 922475108<br>LEI: 549300YZEXPDB92M2F02 | Long             | EC               | CORP              | US        |     10746 | NS      | $1734189.48   | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| SOPHiA GENETICS SA                                | SOPHIA GENETICS SA COMMON STOCK USD.05                 | CUSIP: H82027105<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |     26245 | NS      | $54064.70     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| CureVac N.V.                                      | CUREVAC NV COMMON STOCK EUR.12                         | CUSIP: N2451R105<br>LEI: N/A                  | Long             | EC               | CORP              | DE        |      7800 | NS      | $47034.00     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Immatics N.V.                                     | IMMATICS NV COMMON STOCK                               | CUSIP: N44445109<br>LEI: N/A                  | Long             | EC               | CORP              | DE        |     12569 | NS      | $109475.99    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Seagen Inc.                                       | SEAGEN INC COMMON STOCK USD.001                        | CUSIP: 81181C104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8555 | NS      | $1099403.05   | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Seer Inc.                                         | SEER INC COMMON STOCK USD.00001                        | CUSIP: 81578P106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     19716 | NS      | $114352.80    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Sema4 Holdings Corp                               | SEMA4 HLDGS CORP WTS COMMON STOCK USD.0001             | CUSIP: 81663L101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     30292 | NS      | $7991.03      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Sema4 Holdings Corp                               | SEMA4 HLDGS CORP WTS CALL EXP 22JUL26                  | CUSIP: 81663L119<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4862 | NS      | $92.38        | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| SENTI BIOSCIENCES INC COMMON STOCK USD.0001       | SENTI BIOSCIENCES INC COMMON STOCK                     | CUSIP: 81726A100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18789 | NS      | $26492.49     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| ShockWave Medical Inc.                            | SHOCKWAVE MEDICAL INC COMMON STOCK USD.001             | CUSIP: 82489T104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10170 | NS      | $2091053.70   | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| SomaLogic Inc.                                    | SOMALOGIC INC WTS CALL EXP 31AUG26                     | CUSIP: 83444K113<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2050 | NS      | $820.00       | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| SpringWorks Therapeutics Inc.                     | SPRINGWORKS THERAPEUTICS INC COMMON STOCK USD.0001     | CUSIP: 85205L107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2981 | NS      | $77535.81     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| STERIS plc                                        | STERIS PLC COMMON STOCK USD75.0                        | CUSIP: 859152100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4491 | NS      | $829442.79    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Stoke Therapeutics Inc.                           | STOKE THERAPEUTICS INC COMMON STOCK                    | CUSIP: 86150R107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8627 | NS      | $79627.21     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Stryker Corporation                               | STRYKER CORP COMMON STOCK USD.1                        | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |     26031 | NS      | $6364319.19   | 2.29%             |  |  |  | No            |                  1 | On Loan: No      |
| Adaptive Biotechnologies Corporation              | ADAPTIVE BIOTECHNOLOGIES CORP COMMON STOCK             | CUSIP: 00650F109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     25114 | NS      | $191870.96    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Agilent Technologies Inc.                         | AGILENT TECHNOLOGIES INC COMMON STOCK USD.01           | CUSIP: 00846U101<br>LEI: QUIX8Y7A2WP0XRMW7G29 | Long             | EC               | CORP              | US        |     37413 | NS      | $5598855.45   | 2.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Agios Pharmaceuticals Inc.                        | AGIOS PHARMACEUTICALS INC COMMON STOCK USD.001         | CUSIP: 00847X104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9085 | NS      | $255106.80    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Agilon Health Inc.                                | AGILON HEALTH INC COMMON STOCK USD.01                  | CUSIP: 00857U107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13032 | NS      | $210336.48    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Akero Therapeutics Inc.                           | AKERO THERAPEUTICS INC COMMON STOCK                    | CUSIP: 00973Y108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12068 | NS      | $661326.40    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Alector Inc.                                      | ALECTOR INC COMMON STOCK USD.0001                      | CUSIP: 014442107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11904 | NS      | $109873.92    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Alignment Healthcare Inc.                         | ALIGNMENT HEALTHCARE INC COMMON STOCK                  | CUSIP: 01625V104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     25979 | NS      | $305513.04    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Allakos Inc.                                      | ALLAKOS INC COMMON STOCK USD.001                       | CUSIP: 01671P100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     19563 | NS      | $164720.46    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Allogene Therapeutics Inc.                        | ALLOGENE THERAPEUTICS INC COMMON STOCK USD.001         | CUSIP: 019770106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     37654 | NS      | $236843.66    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Alnylam Pharmaceuticals Inc.                      | ALNYLAM PHARMACEUTICALS INC COMMON STOCK USD.01        | CUSIP: 02043Q107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26360 | NS      | $6264454.00   | 2.25%             |  |  |  | No            |                  1 | On Loan: No      |
| AmerisourceBergen Corporation                     | AMERISOURCEBERGEN CORP COMMON STOCK USD.01             | CUSIP: 03073E105<br>LEI: AI8GXW8LG5WK7E9UD086 | Long             | EC               | CORP              | US        |      2229 | NS      | $369367.59    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                           | INTUITIVE SURGICAL INC COMMON STOCK USD.001            | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |     31476 | NS      | $8352156.60   | 3.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Ionis Pharmaceuticals Inc.                        | IONIS PHARMACEUTICALS INC COMMON STOCK USD.001         | CUSIP: 462222100<br>LEI: 549300SI4ZGLG0BLUZ92 | Long             | EC               | CORP              | US        |     23833 | NS      | $900172.41    | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Iovance Biotherapeutics Inc.                      | IOVANCE BIOTHERAPEUTICS INC COMMON STOCK USD.000041666 | CUSIP: 462260100<br>LEI: 549300220Z2GHH33XL82 | Long             | EC               | CORP              | US        |     34648 | NS      | $221400.72    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| IQVIA Holdings Inc.                               | IQVIA HOLDINGS INC COMMON STOCK USD.01                 | CUSIP: 46266C105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1890 | NS      | $387242.10    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| IVERIC bio Inc.                                   | IVERIC BIO INC COMMON STOCK USD.001                    | CUSIP: 46583P102<br>LEI: 549300GZXI59U71XPM70 | Long             | EC               | CORP              | US        |     35145 | NS      | $752454.45    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Johnson and Johnson                               | JOHNSON + JOHNSON COMMON STOCK USD1.0                  | CUSIP: 478160104<br>LEI: 549300G0CFPGEF6X2043 | Long             | EC               | CORP              | US        |      9091 | NS      | $1605925.15   | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Karuna Therapeutics Inc.                          | KARUNA THERAPEUTICS INC COMMON STOCK                   | CUSIP: 48576A100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10547 | NS      | $2072485.50   | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| Keros Therapeutics Inc.                           | KEROS THERAPEUTICS INC COMMON STOCK USD.0001           | CUSIP: 492327101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      1900 | NS      | $91238.00     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Kodiak Sciences Inc.                              | KODIAK SCIENCES INC COMMON STOCK USD.0001              | CUSIP: 50015M109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9449 | NS      | $67654.84     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Kronos Bio Inc.                                   | KRONOS BIO INC COMMON STOCK                            | CUSIP: 50107A104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2083 | NS      | $3374.46      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Kymera Therapeutics Inc.                          | KYMERA THERAPEUTICS INC COMMON STOCK                   | CUSIP: 501575104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     14979 | NS      | $373875.84    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Incyte Corporation                                | INCYTE CORP COMMON STOCK USD.001                       | CUSIP: 45337C102<br>LEI: 549300Z4WN6JVZ3T4680 | Long             | EC               | CORP              | US        |     16273 | NS      | $1307047.36   | 0.47%             |  |  |  | No            |                  1 | On Loan: No      |
| Insmed Incorporated                               | INSMED INC COMMON STOCK USD.01                         | CUSIP: 457669307<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     46329 | NS      | $925653.42    | 0.33%             |  |  |  | No            |                  1 | On Loan: No      |
| Insulet Corporation                               | INSULET CORP COMMON STOCK USD.001                      | CUSIP: 45784P101<br>LEI: 549300TZTYD2PYN92D43 | Long             | EC               | CORP              | US        |      7578 | NS      | $2230887.42   | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Intellia Therapeutics Inc.                        | INTELLIA THERAPEUTICS INC COMMON STOCK USD.0001        | CUSIP: 45826J105<br>LEI: 5493002T5BCPE5DZC723 | Long             | EC               | CORP              | US        |      7787 | NS      | $271688.43    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Genmab A/S                                        | GENMAB A/S COMMON STOCK DKK1.0                         | CUSIP: 459573903<br>LEI: N/A                  | Long             | EC               | CORP              | DK        |      5647 | NS      | $2387522.16   | 0.86%             |  |  |  | No            |                  2 | On Loan: No      |
| Eisai Co. Ltd.                                    | EISAI CO LTD COMMON STOCK                              | CUSIP: 630720001<br>LEI: N/A                  | Long             | EC               | CORP              | JP        |      9700 | NS      | $639734.72    | 0.23%             |  |  |  | No            |                  2 | On Loan: No      |
| Neurocrine Biosciences Inc.                       | NEUROCRINE BIOSCIENCES INC COMMON STOCK USD.001        | CUSIP: 64125C109<br>LEI: 549300FECER0XBN49756 | Long             | EC               | CORP              | US        |      6983 | NS      | $834049.52    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Nevro Corp.                                       | NEVRO CORP COMMON STOCK USD.001                        | CUSIP: 64157F103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      4319 | NS      | $171032.40    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvalent Inc.                                     | NUVALENT INC COMMON STOCK USD.0001                     | CUSIP: 670703107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2372 | NS      | $70638.16     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Nurix Therapeutics Inc.                           | NURIX THERAPEUTICS INC COMMON STOCK                    | CUSIP: 67080M103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10120 | NS      | $111117.60    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Nuvation Bio Inc.                                 | NUVATION BIO INC COMMON STOCK USD.0001                 | CUSIP: 67080N101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10300 | NS      | $19776.00     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Oak Street Health Inc.                            | OAK STREET HEALTH INC COMMON STOCK USD.001             | CUSIP: 67181A107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9398 | NS      | $202150.98    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Olink Holding AB                                  | OLINK HOLDING AB ADR                                   | CUSIP: 680710100<br>LEI: N/A                  | Long             | EC               | CORP              | SE        |     37166 | NS      | $943273.08    | 0.34%             |  |  |  | No            |                  1 | On Loan: No      |
| Option Care Health Inc.                           | OPTION CARE HEALTH INC COMMON STOCK USD.0001           | CUSIP: 68404L201<br>LEI: 549300DI3Q5ACSYJRI17 | Long             | EC               | CORP              | US        |     13198 | NS      | $397127.82    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| PTC Therapeutics Inc.                             | PTC THERAPEUTICS INC COMMON STOCK USD.001              | CUSIP: 69366J200<br>LEI: 549300UJLWOIWFDGB318 | Long             | EC               | CORP              | US        |      5143 | NS      | $196308.31    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Pacific Biosciences of California Inc.            | PACIFIC BIOSCI OF CALIFORNIA COMMON STOCK USD.0001     | CUSIP: 69404D108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     54894 | NS      | $449032.92    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Penumbra Inc.                                     | PENUMBRA INC COMMON STOCK USD.001                      | CUSIP: 70975L107<br>LEI: 549300RGE06P3ECANU72 | Long             | EC               | CORP              | US        |     11826 | NS      | $2630811.96   | 0.95%             |  |  |  | No            |                  1 | On Loan: No      |
| Roche Holding AG                                  | ROCHE HOLDING AG COMMON STOCK                          | CUSIP: 711038901<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |      7117 | NS      | $2236427.11   | 0.80%             |  |  |  | No            |                  2 | On Loan: No      |
| Elevance Health Inc.                              | ANTHEM INC COMMON STOCK USD.01                         | CUSIP: 036752103<br>LEI: 8MYN82XMYQH89CTMTH67 | Long             | EC               | CORP              | US        |     17061 | NS      | $8751781.17   | 3.15%             |  |  |  | No            |                  1 | On Loan: No      |
| Apellis Pharmaceuticals Inc.                      | APELLIS PHARMACEUTICALS INC COMMON STOCK USD.0001      | CUSIP: 03753U106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13887 | NS      | $718096.77    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Ardelyx Inc.                                      | ARDELYX INC COMMON STOCK USD.000001                    | CUSIP: 039697107<br>LEI: 549300F542QR4SXHCY32 | Long             | EC               | CORP              | US        |     25900 | NS      | $73815.00     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Arcus Biosciences Inc.                            | ARCUS BIOSCIENCES INC COMMON STOCK USD.0001            | CUSIP: 03969F109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8005 | NS      | $165543.40    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| argenx SE                                         | ARGENX SE ADR                                          | CUSIP: 04016X101<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |     12399 | NS      | $4697113.17   | 1.69%             |  |  |  | No            |                  1 | On Loan: No      |
| Arvinas Inc.                                      | ARVINAS INC COMMON STOCK USD.001                       | CUSIP: 04335A105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11123 | NS      | $380517.83    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Ascendis Pharma A/S                               | ASCENDIS PHARMA A/S ADR DKK1.0                         | CUSIP: 04351P101<br>LEI: N/A                  | Long             | EC               | CORP              | DK        |     15465 | NS      | $1888740.45   | 0.68%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                                   | ASTRAZENECA PLC ADR                                    | CUSIP: 046353108<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |    139352 | NS      | $9448065.60   | 3.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Aura Biosciences Inc.                             | AURA BIOSCIENCES INC COMMON STOCK USD.00001            | CUSIP: 05153U107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10426 | NS      | $109473.00    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Avantor Inc.                                      | AVANTOR INC COMMON STOCK USD.01                        | CUSIP: 05352A100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     78069 | NS      | $1646475.21   | 0.59%             |  |  |  | No            |                  1 | On Loan: No      |
| Avidity Biosciences Inc.                          | AVIDITY BIOSCIENCES INC COMMON STOCK                   | CUSIP: 05370A108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     34922 | NS      | $774919.18    | 0.28%             |  |  |  | No            |                  1 | On Loan: No      |
| Becton Dickinson and Company                      | BECTON DICKINSON AND CO COMMON STOCK USD1.0            | CUSIP: 075887109<br>LEI: ICE2EP6D98PQUILVRZ91 | Long             | EC               | CORP              | US        |     19317 | NS      | $4912313.10   | 1.77%             |  |  |  | No            |                  1 | On Loan: No      |
| BeiGene Ltd.                                      | BEIGENE LTD ADR USD.0001                               | CUSIP: 07725L102<br>LEI: 549300JFUK6FRD5MH739 | Long             | EC               | CORP              | US        |      3085 | NS      | $678514.90    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Bicycle Therapeutics PLC                          | BICYCLE THERAPEUTICS PLC ADR USD.01                    | CUSIP: 088786108<br>LEI: N/A                  | Long             | EC               | CORP              | GB        |      7229 | NS      | $213978.40    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| BioMarin Pharmaceutical Inc.                      | BIOMARIN PHARMACEUTICAL INC COMMON STOCK USD.001       | CUSIP: 09061G101<br>LEI: NSLL8ITTRR0J5HEMR848 | Long             | EC               | CORP              | US        |     10723 | NS      | $1109723.27   | 0.40%             |  |  |  | No            |                  1 | On Loan: No      |
| Lyell Immunopharma Inc.                           | LYELL IMMUNOPHARMA INC COMMON STOCK USD.0001           | CUSIP: 55083R104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     41100 | NS      | $142617.00    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Evotec SE                                         | EVOTEC SE COMMON STOCK                                 | CUSIP: 581191905<br>LEI: N/A                  | Long             | EC               | CORP              | DE        |     12592 | NS      | $204848.14    | 0.07%             |  |  |  | No            |                  2 | On Loan: No      |
| McKesson Corporation                              | MCKESSON CORP COMMON STOCK USD.01                      | CUSIP: 58155Q103<br>LEI: 549300WZWOM80UCFSF54 | Long             | EC               | CORP              | US        |       993 | NS      | $372494.16    | 0.13%             |  |  |  | No            |                  1 | On Loan: No      |
| Sartorius AG                                      | SARTORIUS AG PREFERENCE                                | CUSIP: 584332902<br>LEI: N/A                  | Long             | EP               | CORP              | DE        |      4432 | NS      | $1750088.61   | 0.63%             |  |  |  | No            |                  2 | On Loan: No      |
| Merck and Co. Inc.                                | MERCK and CO INC COMMON STOCK USD.5                    | CUSIP: 58933Y105<br>LEI: 4YV9Y5M8S0BRK1RP0397 | Long             | EC               | CORP              | US        |     87155 | NS      | $9669847.25   | 3.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Mettler-Toledo International Inc.                 | METTLER-TOLEDO INTERNATIONAL I COMMON STOCK USD.01     | CUSIP: 592688105<br>LEI: 5493000BD5GJNUDIUG10 | Long             | EC               | CORP              | US        |       914 | NS      | $1321141.30   | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Mirati Therapeutics Inc.                          | MIRATI THERAPEUTICS INC COMMON STOCK                   | CUSIP: 60468T105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10986 | NS      | $497775.66    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Moderna Inc.                                      | MODERNA INC COMMON STOCK USD.0001                      | CUSIP: 60770K107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18517 | NS      | $3326023.54   | 1.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Molina Healthcare Inc.                            | MOLINA HEALTHCARE INC COMMON STOCK USD.001             | CUSIP: 60855R100<br>LEI: 549300NQQCEQ46YHZ591 | Long             | EC               | CORP              | US        |     12901 | NS      | $4260168.22   | 1.53%             |  |  |  | No            |                  1 | On Loan: No      |
| Monte Rosa Therapeutics Inc.                      | MONTE ROSA THERAPEUTICS INC COMMON STOCK USD.0001      | CUSIP: 61225M102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     27152 | NS      | $206626.72    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| MoonLake Immunotherapeutics                       | MOONLAKE IMMUNOTHERAPEUTICS COMMON STOCK USD.0001      | CUSIP: 61559X104<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |      8947 | NS      | $93943.50     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| Morphic Holding Inc.                              | MORPHIC HOLDING INC COMMON STOCK USD.0001              | CUSIP: 61775R105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5689 | NS      | $152180.75    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Pfizer Inc.                                       | PFIZER INC COMMON STOCK USD.05                         | CUSIP: 717081103<br>LEI: 765LHXWGK1KXCLTFYQ30 | Long             | EC               | CORP              | US        |    123263 | NS      | $6315996.12   | 2.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Pliant Therapeutics Inc.                          | PLIANT THERAPEUTICS INC COMMON STOCK                   | CUSIP: 729139105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5681 | NS      | $109813.73    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Prelude Therapeutics Incorporated                 | PRELUDE THERAPEUTICS INC COMMON STOCK                  | CUSIP: 74065P101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7294 | NS      | $44055.76     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Prometheus Biosciences Inc.                       | PROMETHEUS BIOSCIENCES INC COMMON STOCK USD.0001       | CUSIP: 74349U108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5629 | NS      | $619190.00    | 0.22%             |  |  |  | No            |                  1 | On Loan: No      |
| PROGENIC PHARMACEUTICALS INC. COMMON STOCK        | PROGENIC PHARMACEUTICALS INC. COMMON STOCK             | CUSIP: 743CVR037<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21900 | NS      | $25204.71     | 0.01%             |  |  |  | No            |                  3 | On Loan: No      |
| Quanterix Corporation                             | QUANTERIX CORP COMMON STOCK USD.001                    | CUSIP: 74766Q101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7553 | NS      | $104609.05    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Rapid Micro Biosystems Inc.                       | RAPID MICRO BIOSYSTEMS INC COMMON STOCK USD.01         | CUSIP: 75340L104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15325 | NS      | $17317.25     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| RAPT Therapeutics Inc.                            | RAPT THERAPEUTICS INC COMMON STOCK                     | CUSIP: 75382E109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15712 | NS      | $311097.60    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Regeneron Pharmaceuticals Inc.                    | REGENERON PHARMACEUTICALS INC COMMON STOCK USD.001     | CUSIP: 75886F107<br>LEI: 549300RCBFWIRX3HYQ56 | Long             | EC               | CORP              | US        |     11653 | NS      | $8407522.97   | 3.02%             |  |  |  | No            |                  1 | On Loan: No      |
| REGENXBIO Inc.                                    | REGENXBIO INC COMMON STOCK USD.0001                    | CUSIP: 75901B107<br>LEI: 549300EOES47LATSET33 | Long             | EC               | CORP              | US        |      4300 | NS      | $97524.00     | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Relay Therapeutics Inc.                           | RELAY THERAPEUTICS INC COMMON STOCK                    | CUSIP: 75943R102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21413 | NS      | $319910.22    | 0.12%             |  |  |  | No            |                  1 | On Loan: No      |
| Relmada Therapeutics Inc.                         | RELMADA THERAPEUTICS INC COMMON STOCK                  | CUSIP: 75955J402<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      2400 | NS      | $8376.00      | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| C4 Therapeutics Inc.                              | C4 THERAPEUTICS INC COMMON STOCK                       | CUSIP: 12529R107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     19141 | NS      | $112931.90    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Cigna Corporation                                 | CIGNA CORP COMMON STOCK USD.01                         | CUSIP: 125523100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12281 | NS      | $4069186.54   | 1.46%             |  |  |  | No            |                  1 | On Loan: No      |
| Catalent Inc.                                     | CATALENT INC COMMON STOCK USD.01                       | CUSIP: 148806102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18227 | NS      | $820397.27    | 0.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Celldex Therapeutics Inc.                         | CELLDEX THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 15117B202<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12361 | NS      | $550929.77    | 0.20%             |  |  |  | No            |                  1 | On Loan: No      |
| Centene Corporation                               | CENTENE CORP COMMON STOCK USD.001                      | CUSIP: 15135B101<br>LEI: 549300Z7JJ4TQSQGT333 | Long             | EC               | CORP              | US        |     58496 | NS      | $4797256.96   | 1.73%             |  |  |  | No            |                  1 | On Loan: No      |
| Centessa Pharmaceuticals PLC                      | CENTESSA PHARMACEUTICALS PLC ADR USD.0001              | CUSIP: 152309100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     18009 | NS      | $55827.90     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Cerevel Therapeutics Holdings Inc.                | CEREVEL THERAPEUTICS HOLDING COMMON STOCK USD.0001     | CUSIP: 15678U128<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     32986 | NS      | $1040378.44   | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Certara Inc.                                      | CERTARA INC COMMON STOCK USD.01                        | CUSIP: 15687V109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     17771 | NS      | $285579.97    | 0.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Charles River Laboratories International Inc.     | CHARLES RIVER LABORATORIES INT COMMON STOCK USD.01     | CUSIP: 159864107<br>LEI: 549300BSQ0R4UZ5KX287 | Long             | EC               | CORP              | US        |      5417 | NS      | $1180364.30   | 0.42%             |  |  |  | No            |                  1 | On Loan: No      |
| CinCor Pharma Inc.                                | CINCOR PHARMA INC COMMON STOCK USD.00001               | CUSIP: 17240Y109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     11874 | NS      | $145931.46    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| The Cooper Companies Inc.                         | COOPER COS INC/THE COMMON STOCK USD.1                  | CUSIP: 216648402<br>LEI: QJKMVPIGLH7530PCUE20 | Long             | EC               | CORP              | US        |      2239 | NS      | $740370.13    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Prime Medicine Inc.                               | PRIME MEDICINE INC COMMON STOCK USD.00001              | CUSIP: 74168J101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12784 | NS      | $237526.72    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| PROCEPT BioRobotics Corporation                   | PROCEPT BIOROBOTICS CORP COMMON STOCK USD.00001        | CUSIP: 74276L105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12803 | NS      | $531836.62    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Ventyx Biosciences Inc.                           | VENTYX BIOSCIENCES INC COMMON STOCK USD.0001           | CUSIP: 92332V107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5963 | NS      | $195526.77    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated               | VERTEX PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |     21827 | NS      | $6303201.06   | 2.27%             |  |  |  | No            |                  1 | On Loan: No      |
| Viatris Inc                                       | VIATRIS INC COMMON STOCK                               | CUSIP: 92556V106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     33880 | NS      | $377084.40    | 0.14%             |  |  |  | No            |                  1 | On Loan: No      |
| Voyager Therapeutics Inc.                         | VOYAGER THERAPEUTICS INC COMMON STOCK USD.001          | CUSIP: 92915B106<br>LEI: 54930082S4XVR0ZWCC74 | Long             | EC               | CORP              | US        |     10500 | NS      | $64050.00     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| Warby Parker Inc.                                 | WARBY PARKER INC COMMON STOCK USD.0001                 | CUSIP: 93403J106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     36765 | NS      | $495959.85    | 0.18%             |  |  |  | No            |                  1 | On Loan: No      |
| Waters Corporation                                | WATERS CORP COMMON STOCK USD.01                        | CUSIP: 941848103<br>LEI: 5T547R1474YC9HOD8Q74 | Long             | EC               | CORP              | US        |      1547 | NS      | $529971.26    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| ACERTA FUTURE PAYMENTS PP TC30M7746               | ACERTA FUTURE PAYMENTS PP TC30M7746                    | CUSIP: N/A<br>LEI: N/A                        | Long             | EC               | CORP              | US        |    440804 | NS      | $396723.86    | 0.14%             |  |  |  | Yes           |                  3 | On Loan: No      |
| West Pharmaceutical Services Inc.                 | WEST PHARMACEUTICAL SERVICES I COMMON STOCK USD.25     | CUSIP: 955306105<br>LEI: 5493007NKRGM1RJQ1P16 | Long             | EC               | CORP              | US        |      6500 | NS      | $1529775.00   | 0.55%             |  |  |  | No            |                  1 | On Loan: No      |
| Wuxi Biologics (Cayman) Inc.                      | WUXI BIOLOGICS CAYMAN INC COMMON STOCK USD.00001       | CUSIP: 967JXD907<br>LEI: N/A                  | Long             | EC               | CORP              | CN        |     57500 | NS      | $435499.52    | 0.16%             |  |  |  | No            |                  2 | On Loan: No      |
| Xencor Inc.                                       | XENCOR INC COMMON STOCK USD.01                         | CUSIP: 98401F105<br>LEI: 549300V5IF65437JKG30 | Long             | EC               | CORP              | US        |     16855 | NS      | $438904.20    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Zai Lab Limited                                   | ZAI LAB LTD ADR USD.00006                              | CUSIP: 98887Q104<br>LEI: N/A                  | Long             | EC               | CORP              | CN        |      8301 | NS      | $254840.70    | 0.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Zentalis Pharmaceuticals Inc.                     | ZENTALIS PHARMACEUTICALS INC COMMON STOCK              | CUSIP: 98943L107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     15240 | NS      | $306933.60    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |
| Zimmer Biomet Holdings Inc.                       | ZIMMER BIOMET HOLDINGS INC COMMON STOCK USD.01         | CUSIP: 98956P102<br>LEI: 2P2YLDVPES3BXQ1FRB91 | Long             | EC               | CORP              | US        |      8079 | NS      | $1030072.50   | 0.37%             |  |  |  | No            |                  1 | On Loan: No      |
| Zoetis Inc.                                       | ZOETIS INC COMMON STOCK USD.01                         | CUSIP: 98978V103<br>LEI: 549300HD9Q1LOC9KLJ48 | Long             | EC               | CORP              | US        |      6681 | NS      | $979100.55    | 0.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Daiichi Sankyo Company Limited                    | DAIICHI SANKYO CO LTD COMMON STOCK                     | CUSIP: B0J7D9901<br>LEI: N/A                  | Long             | EC               | CORP              | JP        |     72200 | NS      | $2323879.05   | 0.84%             |  |  |  | No            |                  2 | On Loan: No      |
| Biohaven Ltd.                                     | BIOHAVEN LTD COMMON STOCK                              | CUSIP: G1110E107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     12609 | NS      | $175012.92    | 0.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Health Sciences Acquisitions Corporation 2        | HEALTH SCIENCES ACQUISITIONS C COMMON STOCK USD.0001   | CUSIP: G4411D109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     13354 | NS      | $133406.46    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| MeiraGTx Holdings plc                             | MEIRAGTX HOLDINGS PLC COMMON STOCK USD.00001           | CUSIP: G59665102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     10086 | NS      | $65760.72     | 0.02%             |  |  |  | No            |                  1 | On Loan: No      |
| NovoCure Limited                                  | NOVOCURE LTD COMMON STOCK                              | CUSIP: G6674U108<br>LEI: N/A                  | Long             | EC               | CORP              | JE        |     21929 | NS      | $1608492.15   | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Prothena Corporation Public Limited Company       | PROTHENA CORP PLC COMMON STOCK USD.01                  | CUSIP: G72800108<br>LEI: N/A                  | Long             | EC               | CORP              | IE        |     11810 | NS      | $711552.50    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| ADC Therapeutics SA                               | ADC THERAPEUTICS SA COMMON STOCK USD.00001             | CUSIP: H0036K147<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |     23188 | NS      | $89041.92     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| CRISPR Therapeutics AG                            | CRISPR THERAPEUTICS AG COMMON STOCK CHF.03             | CUSIP: H17182108<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |      5251 | NS      | $213453.15    | 0.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Biogen Inc.                                       | BIOGEN INC COMMON STOCK USD.0005                       | CUSIP: 09062X103<br>LEI: W8J5WZB5IY3K0NDQT671 | Long             | EC               | CORP              | US        |      6612 | NS      | $1830995.04   | 0.66%             |  |  |  | No            |                  1 | On Loan: No      |
| Bio-Techne Corporation                            | BIO-TECHNE CORP COMMON STOCK USD.01                    | CUSIP: 09073M104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9100 | NS      | $754208.00    | 0.27%             |  |  |  | No            |                  1 | On Loan: No      |
| BioNTech SE                                       | BIONTECH SE ADR                                        | CUSIP: 09075V102<br>LEI: N/A                  | Long             | EC               | CORP              | DE        |     10618 | NS      | $1595035.96   | 0.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Blueprint Medicines Corporation                   | BLUEPRINT MEDICINES CORP COMMON STOCK USD.001          | CUSIP: 09627Y109<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20359 | NS      | $891927.79    | 0.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Azenta Inc.                                       | BROOKS AUTOMATION INC COMMON STOCK USD.01              | CUSIP: 114340102<br>LEI: 35ZN6X1ANPH25YN32Y17 | Long             | EC               | CORP              | US        |      2400 | NS      | $139728.00    | 0.05%             |  |  |  | No            |                  1 | On Loan: No      |
| Bruker Corporation                                | BRUKER CORP COMMON STOCK USD.01                        | CUSIP: 116794108<br>LEI: LEBZ6VLQ6I8XB1NHVY15 | Long             | EC               | CORP              | US        |     34100 | NS      | $2330735.00   | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Burning Rock Biotech Limited                      | BURNING ROCK BIOTECH LTD ADR USD.0002                  | CUSIP: 12233L107<br>LEI: N/A                  | Long             | EC               | CORP              | CN        |     12350 | NS      | $27787.50     | 0.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Aadi Bioscience Inc.                              | AADI BIOSCIENCE INC COMMON STOCK USD.0001              | CUSIP: 00032Q104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      5700 | NS      | $73131.00     | 0.03%             |  |  |  | No            |                  1 | On Loan: No      |
| AbbVie Inc.                                       | ABBVIE INC COMMON STOCK USD.01                         | CUSIP: 00287Y109<br>LEI: FR5LCKFTG8054YNNRU85 | Long             | EC               | CORP              | US        |     29610 | NS      | $4785272.10   | 1.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Acadia Healthcare Company Inc.                    | ACADIA HEALTHCARE CO INC COMMON STOCK USD.01           | CUSIP: 00404A109<br>LEI: 5493005CW985Y9D0NC11 | Long             | EC               | CORP              | US        |      2400 | NS      | $197568.00    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| ACADIA Pharmaceuticals Inc.                       | ACADIA PHARMACEUTICALS INC COMMON STOCK USD.0001       | CUSIP: 004225108<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     28578 | NS      | $454961.76    | 0.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Invivyd Inc.                                      | ADAGIO THERAPEUTICS INC COMMON STOCK USD.0001          | CUSIP: 00534A102<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8613 | NS      | $12919.50     | 0.00%             |  |  |  | No            |                  1 | On Loan: No      |
| Repligen Corporation                              | REPLIGEN CORP COMMON STOCK USD.01                      | CUSIP: 759916109<br>LEI: 54930006QJ0T5AUO1E71 | Long             | EC               | CORP              | US        |      4188 | NS      | $709070.28    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| Royalty Pharma plc                                | ROYALTY PHARMA PLC COMMON STOCK                        | CUSIP: 76028H209<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     30588 | NS      | $1208837.76   | 0.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Replimune Group Inc.                              | REPLIMUNE GROUP INC COMMON STOCK USD.001               | CUSIP: 76029N106<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     26425 | NS      | $718760.00    | 0.26%             |  |  |  | No            |                  1 | On Loan: No      |
| ROWE T PRICE GOV RES FD 20340919 00000.0000000000 | ROWE T PRICE GOV RES FD 20340919 00000.0000000000      | CUSIP: 76105Y109<br>LEI: N/A                  | Long             | STIV             | RF                | US        |   2976023 | NS      | $2976022.98   | 1.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Revolution Medicines Inc.                         | REVOLUTION MEDICINES INC COMMON STOCK                  | CUSIP: 76155X100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     21716 | NS      | $517275.12    | 0.19%             |  |  |  | No            |                  1 | On Loan: No      |
| Rocket Pharmaceuticals Inc.                       | ROCKET PHARMACEUTICALS INC COMMON STOCK USD.01         | CUSIP: 77313F106<br>LEI: 549300R7VV82YUIN4Y93 | Long             | EC               | CORP              | US        |      9847 | NS      | $192705.79    | 0.07%             |  |  |  | No            |                  1 | On Loan: No      |
| State Street Bank and Trust Company               | SSC GOVERNMENT MM GVMXX                                | CUSIP: 7839989D1<br>LEI: 571474TGEMMWANRLN572 | Long             | STIV             | RF                | US        |    669450 | NS      | $669450.04    | 0.24%             |  |  |  | No            |                  1 | On Loan: No      |
| Sage Therapeutics Inc.                            | SAGE THERAPEUTICS INC COMMON STOCK USD.0001            | CUSIP: 78667J108<br>LEI: 5493000JX4BJS9W6CN35 | Long             | EC               | CORP              | US        |     12342 | NS      | $470723.88    | 0.17%             |  |  |  | No            |                  1 | On Loan: No      |
| Sana Biotechnology Inc.                           | SANA BIOTECHNOLOGY INC COMMON STOCK USD.0001           | CUSIP: 799566104<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     28807 | NS      | $113787.65    | 0.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Sarepta Therapeutics Inc.                         | SAREPTA THERAPEUTICS INC COMMON STOCK USD.0001         | CUSIP: 803607100<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      8416 | NS      | $1090545.28   | 0.39%             |  |  |  | No            |                  1 | On Loan: No      |
| Scholar Rock Holding Corporation                  | SCHOLAR ROCK HOLDING CORP COMMON STOCK USD.001         | CUSIP: 80706P103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     33253 | NS      | $300939.65    | 0.11%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2023-01-30

**Name of Applicant:** John Hancock Variable Insurance Trust

**Signature:** /s/Salvatore Schiavone

**Name of Signer:** Salvatore Schiavone

**Title:** Treasurer