# EDGAR Filing Document

**Accession Number:** 0000022370
**File Stem:** 0001193125-25-219564
**Filing Date:** 2025-9
**Character Count:** 40572
**Document Hash:** 87bc6ed6f57ddcfb6a37c677aea10fe2
**Contains OCR:** False
**Source Format:** 

## Filing Content

## Filing Summary
**0001193125-25-219564.hdr.sgml**: 20250926

**ACCESSION NUMBER**: 0001193125-25-219564

**CONFORMED SUBMISSION TYPE**: NPORT-P

**PUBLIC DOCUMENT COUNT**: 3

**CONFORMED PERIOD OF REPORT**: 20250731

**FILED AS OF DATE**: 20250926

**DATE AS OF CHANGE**: 20250926

**PERIOD START**: 20251031

**FILER**: 

**COMPANY DATA:**
- **COMPANY CONFORMED NAME:** JOHN HANCOCK INVESTMENT TRUST
- **CENTRAL INDEX KEY:** 0000022370

**ORGANIZATION NAME:**
- **EIN:** 746035056
- **STATE OF INCORPORATION:** MA
- **FISCAL YEAR END:** 1031

**FILING VALUES:**
- **FORM TYPE:** NPORT-P
- **SEC ACT:** 1940 Act
- **SEC FILE NUMBER:** 811-00560
- **FILM NUMBER:** 251346794

**BUSINESS ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116
- **BUSINESS PHONE:** 617-663-3000

**MAIL ADDRESS:**
- **STREET 1:** C/O JOHN HANCOCK FUNDS
- **STREET 2:** 200 BERKELEY STREET
- **CITY:** BOSTON
- **STATE:** MA
- **ZIP:** 02116

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** HANCOCK JOHN INVESTMENT TRUST /MA/
- **DATE OF NAME CHANGE:** 19950131

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** TRANSAMERICA INVESTMENT TRUST
- **DATE OF NAME CHANGE:** 19950131

**FORMER COMPANY:**
- **FORMER CONFORMED NAME:** CRITERION INCOME TRUST
- **DATE OF NAME CHANGE:** 19890820

## Series and Classes Contracts Data

### John Hancock ESG Large Cap Core Fund (Series ID: S000053961)

| Class ID   | Class Name   | Ticker Symbol   |
|:---|:---|:---|
| C000169807 | Class I      | JHJIX           |
| C000169811 | Class R6     | JHJRX           |
| C000169812 | Class A      | JHJAX           |
| C000169813 | Class C      | JHJCX           |

## Nport-Ex

![](imgbb25eb651.gif)

#### Quarterly portfolio holdings

## John Hancock

## ESG Large Cap Core Fund

## U.S. equity
July 31, 2025

Manulife, Manulife Investments, Stylized M Design, and Manulife Investments & Stylized M Design are trademarks of The Manufacturers Life Insurance Company and John Hancock and the Stylized John Hancock Design are trademarks of John Hancock Life Insurance Company (U.S.A.). Each are used by it and by its affiliates under license.

------

Fund's investments

------

#### As of 7-31-25 (unaudited)

---

| | | |
|:---|:---|:---|
|  | **Shares** | **Value** |
| **Common stocks 99.4%** |  | **$130405560** |
| (Cost $75,213,173) |  |  |
| **Communication services 9.0%** |  | &nbsp;&nbsp;&nbsp;**11822729** |
| **Entertainment 4.2%** |  |  |
| Netflix, Inc. (A) | 2609 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3024875 |
| Spotify Technology SA (A) | 3971 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2487990 |
| **Interactive media and services 4.8%** |  |  |
| Alphabet, Inc., Class A | 32881 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;6309864 |
| **Consumer discretionary 9.7%** |  | &nbsp;&nbsp;&nbsp;**12707157** |
| **Broadline retail 1.1%** |  |  |
| MercadoLibre, Inc. (A) | 595 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1412465 |
| **Hotels, restaurants and leisure 3.1%** |  |  |
| Booking Holdings, Inc. | 249 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1370511 |
| Chipotle Mexican Grill, Inc. (A) | 32650 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1400032 |
| Marriott International, Inc., Class A | 5026 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1326010 |
| **Specialty retail 4.8%** |  |  |
| AutoZone, Inc. (A) | 460 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1733455 |
| The Home Depot, Inc. | 3779 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1388820 |
| The TJX Companies, Inc. | 25131 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3129563 |
| **Textiles, apparel and luxury goods 0.7%** |  |  |
| Lululemon Athletica, Inc. (A) | 4719 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;946301 |
| **Consumer staples 6.2%** |  | &nbsp;&nbsp;&nbsp;&nbsp;**8125522** |
| **Consumer staples distribution and retail 4.1%** |  |  |
| Costco Wholesale Corp. | 3740 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3514254 |
| Sysco Corp. | 22673 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1804771 |
| **Household products 0.8%** |  |  |
| The Procter & Gamble Company | 7329 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1102795 |
| **Personal care products 1.3%** |  |  |
| Unilever PLC, ADR | 29158 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1703702 |
| **Financials 13.2%** |  | &nbsp;&nbsp;&nbsp;**17333596** |
| **Banks 3.9%** |  |  |
| Bank of America Corp. | 50855 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2403916 |
| Fifth Third Bancorp | 28218 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1173022 |
| The PNC Financial Services Group, Inc. | 8364 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1591418 |
| **Capital markets 1.8%** |  |  |
| Intercontinental Exchange, Inc. | 13045 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2411107 |
| **Consumer finance 1.0%** |  |  |
| Ally Financial, Inc. | 32611 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1234326 |
| **Financial services 3.8%** |  |  |
| Mastercard, Inc., Class A | 5122 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2901459 |
| Visa, Inc., Class A | 5966 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2061074 |
| **Insurance 2.7%** |  |  |
| Aflac, Inc. | 12297 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1221830 |
| The Progressive Corp. | 9649 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2335444 |
| **Health care 8.3%** |  | &nbsp;&nbsp;&nbsp;**10832593** |
| **Biotechnology 1.4%** |  |  |
| Vertex Pharmaceuticals, Inc. (A) | 4105 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1875451 |
| **Health care equipment and supplies 3.7%** |  |  |
| Alcon, Inc. | 9411 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;823933 |
| Boston Scientific Corp. (A) | 12181 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1278031 |
| Intuitive Surgical, Inc. (A) | 2033 | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;978056 |

---

2 JOHN HANCOCK ESG LARGE CAP CORE FUND \| QUARTERLY REPORT SEE NOTES TO FUND'S INVESTMENTS

------

---

| | | |
|:---|:---|:---|
|  | **Shares** | **Value** |
| **Health care (continued)** |  |  |
| **Health care equipment and supplies (continued)** |  |  |
| Stryker Corp. | 4508 | $1770427 |
| **Health care providers and services 0.5%** |  |  |
| UnitedHealth Group, Inc. | 2540 | &nbsp;&nbsp;&nbsp;&nbsp;633882 |
| **Life sciences tools and services 0.9%** |  |  |
| Thermo Fisher Scientific, Inc. | 2360 | &nbsp;&nbsp;&nbsp;1103725 |
| **Pharmaceuticals 1.8%** |  |  |
| AstraZeneca PLC, ADR | 23519 | &nbsp;&nbsp;&nbsp;1719004 |
| Novo Nordisk A/S, ADR | 13811 | &nbsp;&nbsp;&nbsp;&nbsp;650084 |
| **Industrials 10.8%** |  | **14231929** |
| **Building products 1.3%** |  |  |
| Trane Technologies PLC | 3951 | &nbsp;&nbsp;&nbsp;1730854 |
| **Construction and engineering 1.0%** |  |  |
| Quanta Services, Inc. | 3242 | &nbsp;&nbsp;&nbsp;1316673 |
| **Electrical equipment 1.9%** |  |  |
| Eaton Corp. PLC | 6561 | &nbsp;&nbsp;&nbsp;2524148 |
| **Ground transportation 1.9%** |  |  |
| JB Hunt Transport Services, Inc. | 5621 | &nbsp;&nbsp;&nbsp;&nbsp;809705 |
| Union Pacific Corp. | 7655 | &nbsp;&nbsp;&nbsp;1699180 |
| **Machinery 0.8%** |  |  |
| Xylem, Inc. | 6907 | &nbsp;&nbsp;&nbsp;&nbsp;998890 |
| **Professional services 0.9%** |  |  |
| Verisk Analytics, Inc. | 4450 | &nbsp;&nbsp;&nbsp;1240260 |
| **Trading companies and distributors 3.0%** |  |  |
| Ferguson Enterprises, Inc. | 7962 | &nbsp;&nbsp;&nbsp;1778153 |
| United Rentals, Inc. | 2417 | &nbsp;&nbsp;&nbsp;2134066 |
| **Information technology 34.5%** |  | **45337588** |
| **IT services 1.2%** |  |  |
| Accenture PLC, Class A | 6139 | &nbsp;&nbsp;&nbsp;1639727 |
| **Semiconductors and semiconductor equipment 13.5%** |  |  |
| Applied Materials, Inc. | 7443 | &nbsp;&nbsp;&nbsp;1340187 |
| ASML Holding NV, NYRS | 1880 | &nbsp;&nbsp;&nbsp;1306055 |
| NVIDIA Corp. | 67123 | 11939168 |
| NXP Semiconductors NV | 7655 | &nbsp;&nbsp;&nbsp;1636409 |
| Taiwan Semiconductor Manufacturing Company, Ltd., ADR | 6311 | &nbsp;&nbsp;&nbsp;1524864 |
| **Software 14.6%** |  |  |
| Cadence Design Systems, Inc. (A) | 2877 | &nbsp;&nbsp;&nbsp;1048868 |
| Intuit, Inc. | 1822 | &nbsp;&nbsp;&nbsp;1430507 |
| Microsoft Corp. | 22713 | 12117386 |
| Palo Alto Networks, Inc. (A) | 12009 | &nbsp;&nbsp;&nbsp;2084762 |
| ServiceNow, Inc. (A) | 2647 | &nbsp;&nbsp;&nbsp;2496439 |
| **Technology hardware, storage and peripherals 5.2%** |  |  |
| Apple, Inc. | 32631 | &nbsp;&nbsp;&nbsp;6773216 |
| **Materials 3.9%** |  | &nbsp;&nbsp;**5083177** |
| **Chemicals 3.2%** |  |  |
| Ecolab, Inc. | 7136 | &nbsp;&nbsp;&nbsp;1867919 |
| International Flavors & Fragrances, Inc. | 12086 | &nbsp;&nbsp;&nbsp;&nbsp;858469 |
| Linde PLC | 3107 | &nbsp;&nbsp;&nbsp;1430028 |
| **Containers and packaging 0.7%** |  |  |
| Avery Dennison Corp. | 5524 | &nbsp;&nbsp;&nbsp;&nbsp;926761 |

---

SEE NOTES TO FUND'S INVESTMENTS JOHN HANCOCK ESG LARGE CAP CORE FUND \| QUARTERLY REPORT 3

------

---

| | | |
|:---|:---|:---|
|  | **Shares** | **Value** |
| **Real estate 2.1%** |  | **$2724725** |
| **Industrial REITs 0.6%** |  |  |
| Prologis, Inc. | 7463 | &nbsp;&nbsp;&nbsp;&nbsp;796899 |
| **Real estate management and development 0.7%** |  |  |
| Jones Lang LaSalle, Inc. (A) | 3512 | &nbsp;&nbsp;&nbsp;&nbsp;949504 |
| **Specialized REITs 0.8%** |  |  |
| Equinix, Inc. | 1246 | &nbsp;&nbsp;&nbsp;&nbsp;978322 |
| **Utilities 1.7%** |  | &nbsp;&nbsp;**2206544** |
| **Electric utilities 1.1%** |  |  |
| NextEra Energy, Inc. | 20373 | &nbsp;&nbsp;&nbsp;1447705 |
| **Water utilities 0.6%** |  |  |
| American Water Works Company, Inc. | 5411 | &nbsp;&nbsp;&nbsp;&nbsp;758839 |

---

---

| | | | |
|:---|:---|:---|:---|
|  | **Yield (%)** | **Shares** | **Value** |
| **Short-term investments 0.8%** |  |  | **$1069678** |
| (Cost $1,069,678) |  |  |  |
| **Short-term funds 0.8%** |  |  | &nbsp;&nbsp;&nbsp;**1069678** |
| Federated Government Obligations Fund, Institutional Class | 4.1900(B) | 1069678 | &nbsp;&nbsp;&nbsp;1069678 |

---

---

| | |
|:---|:---|
| **Total investments (Cost $76,282,851) 100.2%** | **$131475238** |
| **Other assets and liabilities, net (0.2%)** | &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;**(243138)** |
| **Total net assets 100.0%** | **$131232100** |

---

---

| | |
|:---|:---|
| The percentage shown for each investment category is the total value of the category as a percentage of the net assets of the fund. | The percentage shown for each investment category is the total value of the category as a percentage of the net assets of the fund. |
| **Security Abbreviations and Legend** | **Security Abbreviations and Legend** |
| ADR | American Depositary Receipt |
| NYRS | New York Registry Shares |
| (A) | Non-income producing security. |
| (B) | The rate shown is the annualized seven-day yield as of 7-31-25. |

---

The fund had the following country composition as a percentage of net assets on 7-31-25:

---

| | |
|:---|:---|
| United States | &nbsp;&nbsp;&nbsp;&nbsp;84.7% |
| Ireland | &nbsp;&nbsp;&nbsp;&nbsp;4.5% |
| United Kingdom | &nbsp;&nbsp;&nbsp;&nbsp;2.6% |
| Netherlands | &nbsp;&nbsp;&nbsp;&nbsp;2.2% |
| Sweden | &nbsp;&nbsp;&nbsp;&nbsp;1.9% |
| Taiwan | &nbsp;&nbsp;&nbsp;&nbsp;1.2% |
| Uruguay | &nbsp;&nbsp;&nbsp;&nbsp;1.1% |
| Other countries | &nbsp;&nbsp;&nbsp;&nbsp;1.8% |
| **TOTAL** | **100.0%** |

---

4 JOHN HANCOCK ESG LARGE CAP CORE FUND \| QUARTERLY REPORT SEE NOTES TO FUND'S INVESTMENTS

------

Notes to Fund's investments (unaudited)

**Security valuation. Investments are stated at value as of the scheduled close of regular trading on the New York Stock Exchange (NYSE), normally at 4:00 P.M., Eastern Time. In case of emergency or other disruption resulting in the NYSE not opening for trading or the NYSE closing at a time other than the regularly scheduled close, the net asset value (NAV) may be determined as of the regularly scheduled close of the NYSE pursuant to the Valuation Policies and Procedures of the Advisor, John Hancock Investment Management LLC, the fund's valuation designee.**

In order to value the securities, the fund uses the following valuation techniques: Equity securities, including exchange-traded or closed-end funds, are typically valued at the last sale price or official closing price on the exchange or principal market where the security trades. In the event there were no sales during the day or closing prices are not available, the securities are valued using the last available bid price. Investments by the fund in open-end mutual funds are valued at their respective NAVs each business day.

In certain instances, the Pricing Committee of the Advisor may determine to value equity securities using prices obtained from another exchange or market if trading on the exchange or market on which prices are typically obtained did not open for trading as scheduled, or if trading closed earlier than scheduled, and trading occurred as normal on another exchange or market.

Other portfolio securities and assets, for which reliable market quotations are not readily available, are valued at fair value as determined in good faith by the Pricing Committee following procedures established by the Advisor and adopted by the Board of Trustees. The frequency with which these fair valuation procedures are used cannot be predicted and fair value of securities may differ significantly from the value that would have been used had a ready market for such securities existed.

The fund uses a three tier hierarchy to prioritize the pricing assumptions, referred to as inputs, used in valuation techniques to measure fair value. Level 1 includes securities valued using quoted prices in active markets for identical securities, including registered investment companies. Level 2 includes securities valued using other significant observable inputs. Observable inputs may include quoted prices for similar securities, interest rates, prepayment speeds and credit risk. Prices for securities valued using these inputs are received from independent pricing vendors and brokers and are based on an evaluation of the inputs described. Level 3 includes securities valued using significant unobservable inputs when market prices are not readily available or reliable, including the Advisor's assumptions in determining the fair value of investments. Factors used in determining value may include market or issuer specific events or trends, changes in interest rates and credit quality. The inputs or methodology used for valuing securities are not necessarily an indication of the risks associated with investing in those securities. Changes in valuation techniques and related inputs may result in transfers into or out of an assigned level within the disclosure hierarchy.

As of July 31, 2025, all investments are categorized as Level 1 under the hierarchy described above.

For additional information on the fund's significant accounting policies and risks, please refer to the fund's most recent semiannual or annual shareholder report and prospectus.

\|<sub>5</sub>

## Form NPORT-P: Monthly Portfolio Investments Report

### NPORT-P: Part A: General Information

**Item A.1. Information about the Registrant.**

- **a. Name of Registrant:** John Hancock Investment Trust

- **b. Investment Company Act file number:** 811-00560

- **c. CIK number of Registrant:** 0000022370

- **d. LEI of Registrant:** 549300WRGYBW55IKGU23

- **e. Address and telephone number of Registrant.**

  - **Street Address 1:** 200 Berkeley Street

  - **City:** Boston

  - **State:** MA

  - **Foreign country:** US

  - **Zip / Postal Code:** 02116

  - **Telephone number:** 617-543-9634

**Item A.2. Information about the Series.**

- **a. Name of Series:** ESG Large Cap Core Fund

- **b. EDGAR series identifier (if any):** S000053961

- **c. LEI of Series:** 549300KVWEOXTMVHNB60

**Item A.3. Reporting period.**

- **a. Date of fiscal year-end:** 2025-10-31

- **b. Date as of which information is reported:** 2025-07-31

**Item A.4. Final filing**

Does the Fund anticipate that this will be its final filing on Form N-PORT? **No**

### Fund Information

**Total Assets:** $131601179.59

**Total Liabilities:** $285049.15

**Net Assets:** $131316130.44

**Cash Not Reported:** $0.05

**Monthly Return Information**

| Class               | Month 1 Return (%)   | Month 2 Return (%)   | Month 3 Return (%)   |
|:---|:---|:---|:---|
| Class ID C000169807 | 6.13%                | 3.91%                | -0.29%               |
| Class ID C000169811 | 6.17%                | 3.90%                | -0.33%               |
| Class ID C000169812 | 6.15%                | 3.87%                | -0.34%               |
| Class ID C000169813 | 6.03%                | 3.82%                | -0.39%               |

**Monthly Gains & Losses**

| Period   | Net Realized Gain/Loss   | Net Unrealized Appreciation/Depreciation   |
|:---|:---|:---|
| Month 1  | $1641962.97              | $5740010.54                                |
| Month 2  | $677774.49               | $4196970.42                                |
| Month 3  | $158.23                  | $-380474.63                                |

### Schedule of Portfolio Investments

| Name                                               | Title                                                      | Identifiers                                   | Payoff Profile   | Asset Category   | Issuer Category   | Country   |   Balance | Units   | Value (USD)   | % of Net Assets   | Maturity Date   | Coupon Type   | Annualized Rate (%)   | Restricted?   |   Fair Value Level | Lending Status   |
|:---|:---|:---|:---|:---|:---|:---|---:|:---|:---|:---|:---|:---|:---|:---|---:|:---|
| NXP Semiconductors N.V.                            | NXP SEMICONDUCTORS NV COMMON STOCK EUR.2                   | CUSIP: N6596X109<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |      7655 | NS      | $1636409.35   | 1.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Microsoft Corporation                              | MICROSOFT CORP COMMON STOCK USD.00000625                   | CUSIP: 594918104<br>LEI: INR2EJN1ERAN0W5ZP974 | Long             | EC               | CORP              | US        |     22713 | NS      | $12117385.50  | 9.23%             |  |  |  | No            |                  1 | On Loan: No      |
| Federated Hermes Government Ob                     | FEDERATED HERMES GOVERNMENT OB FH GOVERNMENT OBLIGATION-IS | CUSIP: 60934N104<br>LEI: N/A                  | Long             | STIV             | RF                | US        |   1069678 | NS      | $1069678.00   | 0.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Netflix Inc.                                       | NETFLIX INC COMMON STOCK USD.001                           | CUSIP: 64110L106<br>LEI: 549300Y7VHGU0I7CE873 | Long             | EC               | CORP              | US        |      2609 | NS      | $3024874.60   | 2.30%             |  |  |  | No            |                  1 | On Loan: No      |
| NextEra Energy Inc.                                | NEXTERA ENERGY INC COMMON STOCK USD.01                     | CUSIP: 65339F101<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     20373 | NS      | $1447705.38   | 1.10%             |  |  |  | No            |                  1 | On Loan: No      |
| Booking Holdings Inc.                              | BOOKING HOLDINGS INC COMMON STOCK USD.008                  | CUSIP: 09857L108<br>LEI: FXM8FAOHMYDIPD38UZ17 | Long             | EC               | CORP              | US        |       249 | NS      | $1370510.94   | 1.04%             |  |  |  | No            |                  1 | On Loan: No      |
| Boston Scientific Corporation                      | BOSTON SCIENTIFIC CORP COMMON STOCK USD.01                 | CUSIP: 101137107<br>LEI: Y6ZDD9FP4P8JSSJMW954 | Long             | EC               | CORP              | US        |     12181 | NS      | $1278030.52   | 0.97%             |  |  |  | No            |                  1 | On Loan: No      |
| Cadence Design Systems Inc.                        | CADENCE DESIGN SYSTEMS INC COMMON STOCK USD.01             | CUSIP: 127387108<br>LEI: GCT7RXJOGLXPV0NXZY22 | Long             | EC               | CORP              | US        |      2877 | NS      | $1048867.89   | 0.80%             |  |  |  | No            |                  1 | On Loan: No      |
| Chipotle Mexican Grill Inc.                        | CHIPOTLE MEXICAN GRILL INC COMMON STOCK USD.01             | CUSIP: 169656105<br>LEI: N/A                  | Long             | EC               | CORP              | US        |     32650 | NS      | $1400032.00   | 1.07%             |  |  |  | No            |                  1 | On Loan: No      |
| Verisk Analytics Inc.                              | VERISK ANALYTICS INC COMMON STOCK USD.001                  | CUSIP: 92345Y106<br>LEI: 549300I1YSWNIRKBWP67 | Long             | EC               | CORP              | US        |      4450 | NS      | $1240259.50   | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Vertex Pharmaceuticals Incorporated                | VERTEX PHARMACEUTICALS INC COMMON STOCK USD.01             | CUSIP: 92532F100<br>LEI: 54930015RAQRRZ5ZGJ91 | Long             | EC               | CORP              | US        |      4105 | NS      | $1875451.35   | 1.43%             |  |  |  | No            |                  1 | On Loan: No      |
| Linde Public Limited Company                       | LINDE PLC COMMON STOCK                                     | CUSIP: G54950103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      3107 | NS      | $1430027.82   | 1.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Trane Technologies Public Limited Company          | TRANE TECHNOLOGIES PLC COMMON STOCK USD1.0                 | CUSIP: G8994E103<br>LEI: N/A                  | Long             | EC               | CORP              | IE        |      3951 | NS      | $1730854.08   | 1.32%             |  |  |  | No            |                  1 | On Loan: No      |
| Alcon AG                                           | ALCON AG COMMON STOCK CHF.04                               | CUSIP: H01301128<br>LEI: N/A                  | Long             | EC               | CORP              | CH        |      9411 | NS      | $823933.05    | 0.63%             |  |  |  | No            |                  1 | On Loan: No      |
| Spotify Technology S.A.                            | SPOTIFY TECHNOLOGY SA COMMON STOCK EUR.000625              | CUSIP: L8681T102<br>LEI: N/A                  | Long             | EC               | CORP              | SE        |      3971 | NS      | $2487990.34   | 1.89%             |  |  |  | No            |                  1 | On Loan: No      |
| ASML Holding N.V.                                  | ASML HOLDING NV NY REG SHRS EUR.09                         | CUSIP: N07059210<br>LEI: N/A                  | Long             | EC               | CORP              | NL        |      1880 | NS      | $1306054.80   | 0.99%             |  |  |  | No            |                  1 | On Loan: No      |
| Avery Dennison Corporation                         | AVERY DENNISON CORP COMMON STOCK USD1.0                    | CUSIP: 053611109<br>LEI: 549300PW7VPFCYKLIV37 | Long             | EC               | CORP              | US        |      5524 | NS      | $926761.48    | 0.71%             |  |  |  | No            |                  1 | On Loan: No      |
| Bank of America Corporation                        | BANK OF AMERICA CORP COMMON STOCK USD.01                   | CUSIP: 060505104<br>LEI: 9DJT3UXIJIZJI4WXO774 | Long             | EC               | CORP              | US        |     50855 | NS      | $2403915.85   | 1.83%             |  |  |  | No            |                  1 | On Loan: No      |
| Costco Wholesale Corporation                       | COSTCO WHOLESALE CORP COMMON STOCK USD.005                 | CUSIP: 22160K105<br>LEI: 29DX7H14B9S6O3FD6V18 | Long             | EC               | CORP              | US        |      3740 | NS      | $3514253.60   | 2.68%             |  |  |  | No            |                  1 | On Loan: No      |
| Ecolab Inc.                                        | ECOLAB INC COMMON STOCK USD1.0                             | CUSIP: 278865100<br>LEI: 82DYEISM090VG8LTLS26 | Long             | EC               | CORP              | US        |      7136 | NS      | $1867919.36   | 1.42%             |  |  |  | No            |                  1 | On Loan: No      |
| Equinix Inc.                                       | EQUINIX INC REIT USD.001                                   | CUSIP: 29444U700<br>LEI: 549300EVUN2BTLJ3GT74 | Long             | EC               | CORP              | US        |      1246 | NS      | $978321.82    | 0.75%             |  |  |  | No            |                  1 | On Loan: No      |
| Ferguson Enterprises Inc.                          | FERGUSON ENTERPRISES INC COMMON STOCK USD.0001             | CUSIP: 31488V107<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7962 | NS      | $1778153.46   | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Fifth Third Bancorp                                | FIFTH THIRD BANCORP COMMON STOCK                           | CUSIP: 316773100<br>LEI: THRNG6BD57P9QWTQLG42 | Long             | EC               | CORP              | US        |     28218 | NS      | $1173022.26   | 0.89%             |  |  |  | No            |                  1 | On Loan: No      |
| J.B. Hunt Transport Services Inc.                  | JB HUNT TRANSPORT SERVICES INC COMMON STOCK USD.01         | CUSIP: 445658107<br>LEI: 549300XCD1MPI1C5GK90 | Long             | EC               | CORP              | US        |      5621 | NS      | $809705.05    | 0.62%             |  |  |  | No            |                  1 | On Loan: No      |
| Intercontinental Exchange Inc.                     | INTERCONTINENTAL EXCHANGE INC COMMON STOCK USD.01          | CUSIP: 45866F104<br>LEI: 5493000F4ZO33MV32P92 | Long             | EC               | CORP              | US        |     13045 | NS      | $2411107.35   | 1.84%             |  |  |  | No            |                  1 | On Loan: No      |
| American Water Works Company Inc.                  | AMERICAN WATER WORKS CO INC COMMON STOCK USD.01            | CUSIP: 030420103<br>LEI: 549300DXHIJQMD5WFW18 | Long             | EC               | CORP              | US        |      5411 | NS      | $758838.64    | 0.58%             |  |  |  | No            |                  1 | On Loan: No      |
| Aflac Incorporated                                 | AFLAC INC COMMON STOCK USD.1                               | CUSIP: 001055102<br>LEI: 549300N0B7DOGLXWPP39 | Long             | EC               | CORP              | US        |     12297 | NS      | $1221829.92   | 0.93%             |  |  |  | No            |                  1 | On Loan: No      |
| Ally Financial Inc.                                | ALLY FINANCIAL INC COMMON STOCK USD.01                     | CUSIP: 02005N100<br>LEI: 549300JBN1OSM8YNAI90 | Long             | EC               | CORP              | US        |     32611 | NS      | $1234326.35   | 0.94%             |  |  |  | No            |                  1 | On Loan: No      |
| Alphabet Inc.                                      | ALPHABET INC COMMON STOCK USD.001                          | CUSIP: 02079K305<br>LEI: 5493006MHB84DD0ZWV18 | Long             | EC               | CORP              | US        |     32881 | NS      | $6309863.90   | 4.81%             |  |  |  | No            |                  1 | On Loan: No      |
| Novo Nordisk A/S                                   | NOVO NORDISK A/S ADR                                       | CUSIP: 670100205<br>LEI: 549300DAQ1CVT6CXN342 | Long             | EC               | CORP              | DK        |     13811 | NS      | $650083.77    | 0.50%             |  |  |  | No            |                  1 | On Loan: No      |
| NVIDIA Corporation                                 | NVIDIA CORP COMMON STOCK USD.001                           | CUSIP: 67066G104<br>LEI: 549300S4KLFTLO7GSQ80 | Long             | EC               | CORP              | US        |     67123 | NS      | $11939168.01  | 9.09%             |  |  |  | No            |                  1 | On Loan: No      |
| The PNC Financial Services Group Inc.              | PNC FINANCIAL SERVICES GROUP I COMMON STOCK USD5.0         | CUSIP: 693475105<br>LEI: CFGNEKW0P8842LEUIA51 | Long             | EC               | CORP              | US        |      8364 | NS      | $1591418.28   | 1.21%             |  |  |  | No            |                  1 | On Loan: No      |
| Palo Alto Networks Inc.                            | PALO ALTO NETWORKS INC COMMON STOCK USD.0001               | CUSIP: 697435105<br>LEI: 549300QXR2YVZV231H43 | Long             | EC               | CORP              | US        |     12009 | NS      | $2084762.40   | 1.59%             |  |  |  | No            |                  1 | On Loan: No      |
| The Procter and Gamble Company                     | PROCTER and GAMBLE CO/THE COMMON STOCK                     | CUSIP: 742718109<br>LEI: 2572IBTT8CCZW6AU4141 | Long             | EC               | CORP              | US        |      7329 | NS      | $1102794.63   | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| The Progressive Corporation                        | PROGRESSIVE CORP/THE COMMON STOCK USD1.0                   | CUSIP: 743315103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      9649 | NS      | $2335443.96   | 1.78%             |  |  |  | No            |                  1 | On Loan: No      |
| Prologis Inc.                                      | PROLOGIS INC REIT USD.01                                   | CUSIP: 74340W103<br>LEI: N/A                  | Long             | EC               | CORP              | US        |      7463 | NS      | $796899.14    | 0.61%             |  |  |  | No            |                  1 | On Loan: No      |
| International Flavors and Fragrances Inc.          | INTERNATIONAL FLAVORS and FRAGRA COMMON STOCK USD.125      | CUSIP: 459506101<br>LEI: BZLRL03D3GPGMOGFO832 | Long             | EC               | CORP              | US        |     12086 | NS      | $858468.58    | 0.65%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuit Inc.                                        | INTUIT INC COMMON STOCK USD.01                             | CUSIP: 461202103<br>LEI: VI90HBPH7XSFMB9E4M29 | Long             | EC               | CORP              | US        |      1822 | NS      | $1430506.86   | 1.09%             |  |  |  | No            |                  1 | On Loan: No      |
| Apple Inc.                                         | APPLE INC COMMON STOCK USD.00001                           | CUSIP: 037833100<br>LEI: HWUPKR0MPOU8FGXBT394 | Long             | EC               | CORP              | US        |     32631 | NS      | $6773216.67   | 5.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Applied Materials Inc.                             | APPLIED MATERIALS INC COMMON STOCK USD.01                  | CUSIP: 038222105<br>LEI: 41BNNE1AFPNAZELZ6K07 | Long             | EC               | CORP              | US        |      7443 | NS      | $1340186.58   | 1.02%             |  |  |  | No            |                  1 | On Loan: No      |
| AstraZeneca PLC                                    | ASTRAZENECA PLC ADR                                        | CUSIP: 046353108<br>LEI: PY6ZZQWO2IZFZC3IOL08 | Long             | EC               | CORP              | GB        |     23519 | NS      | $1719003.71   | 1.31%             |  |  |  | No            |                  1 | On Loan: No      |
| AutoZone Inc.                                      | AUTOZONE INC COMMON STOCK USD.01                           | CUSIP: 053332102<br>LEI: GA3JGKJ41LJKXDN23E90 | Long             | EC               | CORP              | US        |       460 | NS      | $1733454.80   | 1.32%             |  |  |  | No            |                  1 | On Loan: No      |
| The Home Depot Inc.                                | HOME DEPOT INC/THE COMMON STOCK USD.05                     | CUSIP: 437076102<br>LEI: QEKMOTMBBKA8I816DO57 | Long             | EC               | CORP              | US        |      3779 | NS      | $1388820.29   | 1.06%             |  |  |  | No            |                  1 | On Loan: No      |
| Intuitive Surgical Inc.                            | INTUITIVE SURGICAL INC COMMON STOCK USD.001                | CUSIP: 46120E602<br>LEI: 54930052SRG011710797 | Long             | EC               | CORP              | US        |      2033 | NS      | $978055.97    | 0.74%             |  |  |  | No            |                  1 | On Loan: No      |
| Jones Lang LaSalle Incorporated                    | JONES LANG LASALLE INC COMMON STOCK USD.01                 | CUSIP: 48020Q107<br>LEI: 6SYKCME112RT8TQUO411 | Long             | EC               | CORP              | US        |      3512 | NS      | $949504.32    | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Lululemon Athletica Inc.                           | LULULEMON ATHLETICA INC COMMON STOCK USD.005               | CUSIP: 550021109<br>LEI: 549300D9GZ4BMLDW5T40 | Long             | EC               | CORP              | CA        |      4719 | NS      | $946301.07    | 0.72%             |  |  |  | No            |                  1 | On Loan: No      |
| Marriott International Inc.                        | MARRIOTT INTERNATIONAL INC/MD COMMON STOCK USD.01          | CUSIP: 571903202<br>LEI: 225YDZ14ZO8E1TXUSU86 | Long             | EC               | CORP              | US        |      5026 | NS      | $1326009.58   | 1.01%             |  |  |  | No            |                  1 | On Loan: No      |
| Mastercard Incorporated                            | MASTERCARD INC COMMON STOCK USD.0001                       | CUSIP: 57636Q104<br>LEI: AR5L2ODV9HN37376R084 | Long             | EC               | CORP              | US        |      5122 | NS      | $2901459.34   | 2.21%             |  |  |  | No            |                  1 | On Loan: No      |
| MercadoLibre Inc.                                  | MERCADOLIBRE INC COMMON STOCK USD.001                      | CUSIP: 58733R102<br>LEI: N/A                  | Long             | EC               | CORP              | UY        |       595 | NS      | $1412464.55   | 1.08%             |  |  |  | No            |                  1 | On Loan: No      |
| Quanta Services Inc.                               | QUANTA SERVICES INC COMMON STOCK USD.00001                 | CUSIP: 74762E102<br>LEI: SHVRXXEACT60MMH07S24 | Long             | EC               | CORP              | US        |      3242 | NS      | $1316673.46   | 1.00%             |  |  |  | No            |                  1 | On Loan: No      |
| ServiceNow Inc.                                    | SERVICENOW INC COMMON STOCK USD.001                        | CUSIP: 81762P102<br>LEI: 549300HJTQM36M0E1G39 | Long             | EC               | CORP              | US        |      2647 | NS      | $2496438.64   | 1.90%             |  |  |  | No            |                  1 | On Loan: No      |
| Stryker Corporation                                | STRYKER CORP COMMON STOCK USD.1                            | CUSIP: 863667101<br>LEI: 5493002F0SC4JTBU5137 | Long             | EC               | CORP              | US        |      4508 | NS      | $1770426.84   | 1.35%             |  |  |  | No            |                  1 | On Loan: No      |
| Sysco Corporation                                  | SYSCO CORP COMMON STOCK USD1.0                             | CUSIP: 871829107<br>LEI: 5RGWIFLMGH3YS7KWI652 | Long             | EC               | CORP              | US        |     22673 | NS      | $1804770.80   | 1.37%             |  |  |  | No            |                  1 | On Loan: No      |
| The TJX Companies Inc.                             | TJX COS INC/THE COMMON STOCK USD1.0                        | CUSIP: 872540109<br>LEI: V167QI9I69W364E2DY52 | Long             | EC               | CORP              | US        |     25131 | NS      | $3129563.43   | 2.38%             |  |  |  | No            |                  1 | On Loan: No      |
| Taiwan Semiconductor Manufacturing Company Limited | TAIWAN SEMICONDUCTOR MANUFACTU ADR                         | CUSIP: 874039100<br>LEI: N/A                  | Long             | EC               | CORP              | TW        |      6311 | NS      | $1524863.82   | 1.16%             |  |  |  | No            |                  1 | On Loan: No      |
| Thermo Fisher Scientific Inc.                      | THERMO FISHER SCIENTIFIC INC COMMON STOCK USD1.0           | CUSIP: 883556102<br>LEI: HCHV7422L5HDJZCRFL38 | Long             | EC               | CORP              | US        |      2360 | NS      | $1103724.80   | 0.84%             |  |  |  | No            |                  1 | On Loan: No      |
| Unilever PLC                                       | UNILEVER PLC ADR                                           | CUSIP: 904767704<br>LEI: 549300MKFYEKVRWML317 | Long             | EC               | CORP              | GB        |     29158 | NS      | $1703701.94   | 1.30%             |  |  |  | No            |                  1 | On Loan: No      |
| Union Pacific Corporation                          | UNION PACIFIC CORP COMMON STOCK USD2.5                     | CUSIP: 907818108<br>LEI: 549300LMMRSZZCZ8CL11 | Long             | EC               | CORP              | US        |      7655 | NS      | $1699180.35   | 1.29%             |  |  |  | No            |                  1 | On Loan: No      |
| United Rentals Inc.                                | UNITED RENTALS INC COMMON STOCK USD.01                     | CUSIP: 911363109<br>LEI: 5323X5O7RN0NKFCDRY08 | Long             | EC               | CORP              | US        |      2417 | NS      | $2134065.98   | 1.63%             |  |  |  | No            |                  1 | On Loan: No      |
| UnitedHealth Group Incorporated                    | UNITEDHEALTH GROUP INC COMMON STOCK USD.01                 | CUSIP: 91324P102<br>LEI: 549300GHBMY8T5GXDE41 | Long             | EC               | CORP              | US        |      2540 | NS      | $633882.40    | 0.48%             |  |  |  | No            |                  1 | On Loan: No      |
| Visa Inc.                                          | VISA INC COMMON STOCK USD.0001                             | CUSIP: 92826C839<br>LEI: 549300JZ4OKEHW3DPJ59 | Long             | EC               | CORP              | US        |      5966 | NS      | $2061074.02   | 1.57%             |  |  |  | No            |                  1 | On Loan: No      |
| Xylem Inc.                                         | XYLEM INC/NY COMMON STOCK                                  | CUSIP: 98419M100<br>LEI: 549300DF5MV96DRYLQ48 | Long             | EC               | CORP              | US        |      6907 | NS      | $998890.34    | 0.76%             |  |  |  | No            |                  1 | On Loan: No      |
| Accenture Public Limited Company                   | ACCENTURE PLC COMMON STOCK USD.0000225                     | CUSIP: G1151C101<br>LEI: N/A                  | Long             | EC               | CORP              | IE        |      6139 | NS      | $1639726.90   | 1.25%             |  |  |  | No            |                  1 | On Loan: No      |
| Eaton Corporation Public Limited Company           | EATON CORP PLC COMMON STOCK USD.01                         | CUSIP: G29183103<br>LEI: 549300VDIGTMXUNT7H71 | Long             | EC               | CORP              | IE        |      6561 | NS      | $2524147.92   | 1.92%             |  |  |  | No            |                  1 | On Loan: No      |

### Signature

**Date Signed:** 2025-08-29

**Name of Applicant:** John Hancock Investment Trust

**Signature:** /s/Salvatore Schiavone

**Name of Signer:** Salvatore Schiavone

**Title:** Treasurer