Company: MBIO
Filing Date: 2025-04-01
Form Type: POS EX
Source: 0001104659-25-030659
Chunk: 0

Company: MUSTANG BIO, INC.
Filing Date: 2025-04-01
Form: POS EX
Chunk 0
---
<div align='center'>As filed with the Securities and Exchange Commission on April 1, 2025.</div>

Registration No. 333-284299

<div align='center'>UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

Post-effective Amendment No. 1 to

FORM S-1

REGISTRATION STATEMENT

UNDER

THE SECURITIES ACT OF 1933

MUSTANG BIO, INC.

(Exact name of registrant as specified in its charter)</div>

| Delaware                        |                         2836 | 47-3828760            |
| (State or Other Jurisdiction of 
 Incorporation or Organization)  | -Primary Standard Industrial 
   Classification Code Number | -I.R.S. Employer      
 Identification Number |

<div align='center'>95 Sawyer Road

Waltham, MA 02453

(781) 652-4500

(Address, including zip code, and telephone number,
including area code, of registrant’s principal executive offices)

Manuel Litchman, M.D.

President, Chief Executive Officer and Interim Chief Financial Officer

Mustang Bio, Inc.

95 Sawyer Road

Waltham, MA 02453

(781) 652-4500

(Name, address, including zip code, and telephone
number, including area code, of agent for service)

Copies to:

Rakesh Gopalan

David S. Wolpa

Alexander T. Yarbrough

Troutman Pepper Locke LLP

301 S. College Street, 34 thFloor

Charlotte, NC 28202

(704) 998-4050</div>

Approximate date of commencement of proposed sale to the public: From time to time after this registration statement becomes effective.

If any of the securities being registered on
this Form are to be offered on a delayed or continuous basis pursuant to Rule 415 under the Securities Act of 1933 check the following
box: x

If this Form is filed to register additional securities
for an offering pursuant to Rule 462(b) under the Securities Act, please check the following box and list the Securities Act registration
statement number of the earlier effective registration statement for the same offering. ¨

If this Form is a post-effective amendment filed
pursuant to Rule 462(c) under the Securities Act, check the following box and list the Securities Act registration statement number of
the earlier effective registration statement for the same offering. �