Company: GANX
Filing Date: 2025-12-03
Form Type: S-8
Source: 0001104659-25-118118
Chunk: 3

Company: Gain Therapeutics, Inc.
Filing Date: 2025-12-03
Form: S-8
Chunk 3
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 No. 333-266142), filed with the Commission on July 15, 2022).                              |
| 10.3    |     | Form of                                                                                                                             
 2022 Equity Incentive Plan Stock Option Award Agreement (incorporated by reference to Exhibit 4.7 to the Company’s Registration     
 Statement on Form S-8 (File No. 333-266142), filed with the Commission on July 15, 2022).                                           |
| 23.1*   |     | Consent of Ernst &                                                                                                                  
 Young AG.                                                                                                                           |
| 23.2*   |     | Consent                                                                                                                             
 of Lowenstein Sandler LLP (included in Exhibit 5.1).                                                                                |
| 24.1*   |     | Power                                                                                                                               
 of Attorney (included on the signature page of this Registration Statement).                                                        |
| 107*    |     | Filing                                                                                                                              
 Fee Table.                                                                                                                          |

* Filed herewith.

<div align='center'>SIGNATURES</div>

Pursuant to the requirements of the Securities Act of 1933, as amended, the registrant certifies that it has reasonable grounds to believe that it meets all of the requirements for filing on Form S-8 and has duly caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Bethesda, Stateof Maryland, on December 3, 2025.

| GAIN THERAPEUTICS, INC. |                         |
| By:                     | /s/ Gene Mack           |
|                         | Gene Mack               |
|                         | Chief Executive Officer |

<div align='center'>POWER OF ATTORNEY</div>

KNOW ALL PERSONS BY
THESE PRESENTS, that each person whose signature appears below constitutes and appoints Gene Mack as attorney-in-fact with full power
of substitution, for him or her in any and all capacities, to do any and all acts and all things and to execute any and all instruments
which said attorneys and agents, or either of them, may deem necessary or desirable to enable the registrant to comply with the Securities
Act of 1933, and any rules, regulations and requirements of the Securities and Exchange Commission thereunder, in connection with the
registration of ordinary shares of the registrant under this registration statement, including, without limitation, the power and authority
to sign the name of each of the undersigned in the capacities indicated below