Company: PRME
Filing Date: 2025-11-07
Form Type: S-3ASR
Source: 0001628280-25-050428
Chunk: 76

Company: Prime Medicine, Inc.
Filing Date: 2025-11-07
Form: S-3ASR
Chunk 76
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 on November 12, 2024)                                |
| 23.1**      |     | Consent of PricewaterhouseCoopers LLP, independent registered public accounting firm                                                                                                                                                                                                          |
| 23.2**      |     | Consent of Goodwin Procter LLP (included in Exhibit 5.1)                                                                                                                                                                                                                                      |
| 23.3**      |     | Consent of Goodwin Procter LLP (included in Exhibit 5.2)                                                                                                                                                                                                                                      |
| 24.1**      |     | Power of Attorney (included in the signature pages to the Registration Statement)                                                                                                                                                                                                             |
| 25.1***     |     | Form T-1 Statement of Eligibility of Trustee for Senior Indenture under the Trust Indenture Act of 1939, as amended                                                                                                                                                                           |
| 25.2***     |     | Form T-1 Statement of Eligibility of Trustee for Senior Indenture under the Trust Indenture Act of 1939, as amended                                                                                                                                                                           |
| 107**       |     | Filing Fee Table                                                                                                                                                                                                                                                                              |

<div align='center'>II-3</div>

| *   |     | To be filed, if necessary, by amendment or as an exhibit to a document to be incorporated or deemed to be incorporated by reference in this registration statement, including a Current Report on Form 8-K.                          |
| **  |     | Filed herewith.                                                                                                                                                                                                                      |
| *** |     | To be filed pursuant to Section 305(b)(2) of the Trust Indenture Act of 1939.                                                                                                                                                        |
| †   |     | Portions of this exhibit (indicated by asterisks) have been omitted in accordance with Item 601(b)(10) of Regulation S-K.                                                                                                            |
| +   |     | Annexes, schedules and/or exhibits have been omitted pursuant to Item 601(a)(5) of Regulation S-K. The Registrant agrees to furnish supplementally a copy of any omitted attachment to the SEC on a confidential basis upon request. |

<div align='center'>II-4</div>

## Item 17. Undertakings.
The undersigned Registrant hereby undertakes:

1. To file, during any period in which offers or sales are being made, a post-effective amendment to this registration statement:

i. to include any prospectus required by Section 10(a)(3) of the Securities Act;

ii. to reflect in the prospectus