Company: NIVFW
Filing Date: 2025-10-31
Form Type: 424B3
Source: 0001213900-25-104469
Chunk: 158

Company: NewGenIvf Group Ltd
Filing Date: 2025-10-31
Form: 424B3
Chunk 158
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, which may include, among other things,
blood pressure, hormone level, ultrasound, infectious disease screening, uterine evaluation and male fertility test. The physician will
then design treatment plans based on the client’s medical history and results of the tests. If the client is satisfied with treatment
plan and the test results are acceptable to the physician, the physician will prescribe medications and start stimulation treatment.

The first step of the cycle
is to boost egg production through injecting synthetic hormones. Over about one week of ovarian stimulation, clients are monitored on
a regular basis with blood test and transvaginal ultrasound. If follicles have reached at least 10 mm in size, an additional antagonist
drug will be added into the daily injection schedule. This is used to prevent ovulation before ovum pickup time. After another few days
of ovarian simulation, if follicle growth is consistent and majority of follicles are around 16 mm to 17 mm, the final injection of a
human chorionic gonadotropin will be administered. The trigger injection is the final step of the stimulation process and is for the
maturation of the eggs in the follicles before they are collected. The next major step is to retrieve the eggs with a minor surgical
procedure called Trans Vaginal Follicle Aspiration conducted under anaesthesia. At the same time the male partner collects the sperms
for fertilizing the eggs in the laboratory by a process known as intracytoplasmic sperm injection. The fertilized embryos are cultured
in the laboratory for two to six days. Embryos that grow well are biopsied and tested by PGT to detect potential genetic diseases.

The final step is to transfer
the embryos into the uterus using a catheter. Within eight days after the embryo transfer, a blood test can be conducted to detect
whether the implantation was successful.

MicroSort Technology

MicroSort technology is a
preconception process developed by the Genetics and IVF Institute, Inc. that aims to improve the chances that the baby to be conceived
will be of the desired gender and prevents certain gender-related hereditary diseases.

Semen samples usually contain
equal amounts of sperm carrying the Y chromosome (which will produce a boy), and sperm carrying the X chromosome (which will produce
a girl). During the MicroSort process, the sperm sample is washed to remove seminal liquid and nonmotile cells. After the washing, the
sample is stained with a special fluorescent material that attaches to the DNA contained in