Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 241

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 241
---
 value on its balance sheet at December 31, 2024 in the amount of $8,908,052.

Procedures for Approval of Related Party Transactions

A “related party transaction”
is any actual or proposed transaction, arrangement or relationship or series of similar transactions, arrangements or relationships, including
those involving indebtedness not in the ordinary course of business, to which we or our subsidiaries were or are a party, or in which
we or our subsidiaries were or are a participant, in which the amount involved exceeded or exceeds the lesser of (i) $120,000 or
(ii) one percent of the average of our total assets at year-end for the last two completed fiscal years and in which any related
party had or will have a direct or indirect material interest. A “related party” includes:

| ● | any person who is, or at any time during the applicable period was, one of our executive officers or one 
 of our directors;                                                                                        |

| ● | any person who beneficially owns more than 5% of our common stock; |

| ● | any immediate family member of any of the foregoing; or |

| ● | any entity in which any of the foregoing is a partner or principal or in a similar position or in which 
 such person has a 10% or greater beneficial ownership interest.                                         |

Our Audit Committee is responsible
for reviewing and approving in advance any related party transaction.

<div align='center'>142

SELLING STOCKHOLDERS</div>

This prospectus covers the resale of up to an aggregate of 42,594,048
shares of common stock, comprising: (a) up to 42,211,548 shares of Common Stock that we may, at our election, issue and sell to Ascent
Partners from time to time pursuant to the Purchase Agreement, subject to the satisfaction of specified conditions; and (b) up to 382,500
shares of Common Stock issued to certain parties in satisfaction of transaction costs incurred in connection with the Business Combination
we completed on July 11, 2025.

The following table sets forth certain information with respect to
the Selling Stockholders, including (i) the shares of our common stock beneficially owned by the Selling Stockholders prior to this
offering, (ii) the number of shares being offered by the Selling Stockholders pursuant to this prospectus and (iii) the Selling
Stockholders’ beneficial ownership after completion of this offering, assuming that all of the shares covered hereby (but