Company: CMND
Filing Date: 2025-01-22
Form Type: 20-F
Source: 0001213900-25-005490
Chunk: 129

Company: Clearmind Medicine Inc.
Filing Date: 2025-01-22
Form: 20-F
Item: Item 4
Chunk 129
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thanolamide (PEA), with the goal to identify the optimal
dosage for this combination and to observe its safety and impact on various metabolic and behavioral parameters, including fat oxidation,
locomotor activity, and feeding behavior.

Fourteen different treatment groups were created
(for a total of 84 animals) receiving single treatment doses ranging from 40, 20, 10, 5, 1, to 0.5 mg/kg of MEAI with or without a constant
PEA dose of 25 mg/kg. MEAI administration exhibited a remarkable degree of tolerance, leaving the animals’ viability unaffected
across all experimental groups. Similar results were also observed in groups treated by the combination of MEAI and PEA with the most
prominent effects being observed when combining MEAI and PEA, particularly at 20 and 10 mg/kg. Results indicated that:

  The administered treatment exhibited a remarkable degree of tolerance, leaving the mices’ viability unaffected across all experimental groups.  

  Combining MEAI and PEA, particularly at 20 and 10 mg/kg, led to increased oxygen consumption and carbon dioxide emission, coupled with elevated energy expenditure and fat oxidation. Oxygen cons...  

  A striking reduction in food consumption (appetite) and meal sizes was also observed, primarily at 40 and 20 mg/kg of MEAI.  

  Slight elevations in carbohydrate oxidation were noted, particularly at 20 and 10 mg/kg.  

  At 40 and 20 mg/kg significant reductions in ambulation was noted, without affecting voluntary activity.  

Collectively, these data provide strong evidence for the metabolism
modulating and anti-obesity effects of MEAI treatment, warranting further preclinical testing.

Bar-Ilan University Study: Elucidation of MEAIs
anti-reward/addictive-like properties

The purpose of this study was to determine MEAI’s
non-rewarding effect on rats and to test whether MEAI is an effective treatment for cocaine preference in rats utilizing the Conditioned
Place Preference (CPP) model. Additionally, the study goal was to test the effect of MEAI on substance-use-disorder (SUD) rat model using
the cocaine self-administration paradigm. Sprague Dawley rats were maintained on a 12 h light/12 h dark reversed cycle, with food and
water available ad libitum. Experiments were conducted during the dark cycle.

Elucidation of MEAI’s Non-Rewarding Effect
on R