Company: NCNA
Filing Date: 2025-06-18
Form Type: F-3/A
Source: 0001193125-25-142565
Chunk: 2

Company: NuCana plc
Filing Date: 2025-06-18
Form: F-3/A
Chunk 2
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No. 1, and (iv) the Amended and Restated Deposit Agreement, by and among the Company, Citibank, N.A., and all holders and beneficial Owners of American Depositary Shares issued thereunder, dated as of May 7, 2025, filed as Exhibit 4.2
to this Amendment No. 1.

Accordingly, conforming updates have been made to (a) update information under the section entitled
“Prospectus Summary” under the headings “About NuCana plc” and “Additional Information”, (b) reference to our most recent Annual Report on Form 20-F and our “Supplemental
Risk Factors” filed with our Form 6-Ks from time to time under the sections entitled “Risk Factors” and “Special Note Regarding Forward-Looking Statements”, (c) update information
under the section entitled “Description of Share Capital” under the headings “Issued Share Capital”, “Ordinary Shares”, “Deferred Shares”, “Options”, and “Articles of Association” related
to our ordinary shares and updated articles of association, (d) reference the Amended Restated Deposit Agreement under the section entitled “Description of American Depositary Shares” and update the information under the heading
“Limitations on Obligations and Liabilities”, (e) update the consent of Ernst & Young LLP, independent registered public accounting firm for the Company, filed as Exhibit 23.1 to the Registration Statement, and (f) update the
opinions of Bristows LLP and Mintz, Levin, Cohn, Ferris, Glovsky and Popeo, P.C., filed as Exhibits 5.1 and 5.2, respectively, to the Registration Statement.

Other than expressly set forth herein, this Amendment No. 1 does not, and does not purport to, amend or restate any other information
contained in the Registration Statement.

The information in this preliminary prospectus is not complete and may be changed. We may not sell these securities until the registration statement filed with the Securities and Exchange Commission is effective. This prospectus is not an offer to sell these securities and it is not soliciting an offer to buy these securities in any state where the offer or sale is not permitted.

SUBJECT TO COMPLETION, DATED JUNE 18, 2025

PRELIMINARY PROSPECTUS

$150,000,000

American Depositary Shares representing Ordinary Shares

Debt Securities

Warrants