Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 193

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 193
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 export. Profusa has also developed Lumee O2 Go, currently for research use only, that operates on a mobile phone
and uploads data to the cloud. This mobile application is being developed to provide real-time visualization of changes in oxygen, data
annotation, and incorporation of 3 party data via Apple’s HealthKit.

The initial glucose professional product,
once approved, is intended to allow physicians and care providers to visualize a patient’s glucose over a series of days. This
first product mobile interface is designed to encourage compliance, log activities, and facilitate correct operation without providing
the patients access to the system’s measured blood glucose values. The second real time use product, once approved, is planned to
allow for users to have real time access to the system’s measured blood glucose values to enable care decisions. An essential feature
of the glucose system is a visualization that allows the patient to correctly locate the reader over the sensor. This mobile user interface
will show a real-time depiction of the reader as it is moved over the sensor in the body to maximize signal quality and optimal position.

Profusa uses Amazon Web Services
(AWS) infrastructure to host and secure data. For authentication and authorization, Profusa’s web application utilizes Amazon Cognito.
To provide network security, data is transmitted over the public network using TLS. All data communication from Profusa’s application
is transmitted through Amazon’s Application Load Balancer which provides both data encryption and allows Profusa’s system
to reside entirely on a private network. Amazon’s systems provide the capabilities to ensure that data is encrypted during transmission
and at rest. Profusa’s mobile applications upload data regularly but do not rely on a network connection to operate. Nonetheless,
to deliver a robust service, Profusa’s cloud infrastructure is hosted on redundant, fault-tolerant application servers, to improve
availability.

The core technology platform
described above is the foundation for Profusa’s future products for the measurement of real time biochemistry, including Lumee Oxygen,
Lumee Glucose. By tailoring the fluorescence chemistry with the optical properties of the multi-channel reader, Profusa believes that
its ability to develop products to measure other analytes and expand our product portfolio is enhanced while maintaining the key value
propositions of the platform. It is conceivable in the future that Profusa may be able to develop a sensor and reader pair to measure
multiple analytes within one product, creating streams of data for a biochemical panel for broader applications.

Short-Term Opportunity with Lumee Oxygen and Lumee