Company: BLLN
Filing Date: 2025-10-07
Form Type: S-1
Source: 0001193125-25-233697
Chunk: 131

Company: BillionToOne, Inc.
Filing Date: 2025-10-07
Form: S-1
Chunk 131
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million, assuming that the number of shares offered by us, as set forth on the cover page of this prospectus, remains the same, and after deducting underwriting discounts and commissions and estimated offering expenses 
 payable by us. Similarly, each 1.0 million share increase or decrease in the number of shares offered by us would increase or decrease, as applicable, pro forma as adjusted cash and cash equivalents, additional              
 paid-in capital, total stockholders’ (deficit) equity and total capitalization by approximately $     million, assuming that the assumed initial offering price to the public                                                   
 remains the same, and after deducting underwriting discounts and commissions and estimated offering expenses payable by us.                                                                                                     |

The number of shares of our common stock issued and outstanding, pro forma and pro forma as adjusted, in the table above is based on 35,616,629 shares of our Class A common stock and 4,552,650 shares of our Class B common stock outstanding as of June 30, 2025, after giving effect to the Preferred Stock Conversion, the Reclassification and the Class B Stock Exchange, in each case as if they had occurred on June 30, 2025, and excludes:

| • |     | 8,930,919 shares of our Class A common stock issuable upon the exercise of stock options outstanding as of 
 June 30, 2025 under our 2018 Plan, at a weighted-average exercise price of $10.59 per share;               |

| • |     | 1,224,549 shares of our Class A common stock issuable upon the exercise of outstanding stock options that were granted 
 subsequent to June 30, 2025 under our 2018 Plan, at a weighted-average exercise price of $28.88 per share;             |

| • |     | up to 9,660 shares of our Class A common stock issuable upon the exercise of the 
 A-6 Warrant for an exercise price of $2.5879 per share;                          |

| • |     | up to 80,357 shares of our Class A common stock issuable upon the exercise of the 2021 Common Warrant for an exercise 
 price of $2.80 per share;                                                                                             |

| • |     | up to 41,209 shares of our Class A common stock issuable upon the exercise of the 2022 Common Warrant for an exercise 
 price of $10.