Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
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Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
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Filed Pursuant to 424(b)(3)

Registration No. 333-290805

<div align='center'>PROFUSA, INC.

42,594,048 Shares of Common Stock</div>

This prospectus relates to the resale, from time to time, by the Selling
Stockholders identified in this prospectus under the caption “Selling Stockholders,” of up to 42,594,048 shares of common
stock, par value $0.0001 per share, of the Company (the “”) comprising: (a) up to 42,211,548 shares of
Common Stock by Ascent Partners Fund LLC, whom we refer to in this prospectus as “Ascent,” pursuant to a common stock purchase
agreement, dated as of July 28, 2025, we entered into with Ascent, which we refer to in this prospectus as the Purchase Agreement; and
(b) up to 382,500 shares of Common Stock issued to certain parties in satisfaction of transaction costs incurred in connection with the
Business Combination we completed on July 11, 2025.

In our registration statement,
filed on August 22, 2025 and declared effective on August 25, 2025, we previously registered the resale of up to 8,970,830 shares by Ascent
pursuant to the Purchase Agreement. This prospectus registers the resale of up to an additional 42,211,548 shares of Common Stock (the
“Purchase Shares”) that we may, in our sole discretion, elect to sell to Ascent, from time to time over a period of up to
36 months from and after the Effective Date (defined below) pursuant to the Purchase Agreement. Ascent is deemed to be an “underwriter”
within the meaning of Section 2(a)(11) of the Securities Act of 1933, as amended, or the Securities Act.

We are registering the offer and sale and/or resale of these securities
to satisfy certain registration obligations we have and certain registration rights we have granted. The Selling Stockholders may offer
all or part of the Resale Securities for resale from time to time through public or private transactions, at either prevailing market
prices or at privately negotiated prices. The Resale Securities are being registered to permit the Selling Stockholders to sell Resale
Securities from time to time, in amounts, at prices and on terms determined at the time of offering. The Selling Stockholders may sell
the Resale