Company: BLLN
Filing Date: 2025-09-17
Form Type: DRS/A
Source: 0001193125-25-206347
Chunk: 237

Company: BillionToOne, Inc.
Filing Date: 2025-09-17
Form: DRS/A
Chunk 237
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 Pending: CN, EU, HK |
| P02    |     | Target-Associated Molecules for Characterization Associated with Biological Targets |     | A method and/or system for facilitating characterization of one or more conditions that can include: generating a set of target-associated molecules; generating a                                                                                  
 reference-associated set of molecules; facilitating generation of a spike-in mixture; determining abundance metrics based on an analysis of the spike-in mixture; and                                                                               
 facilitating the characterization of the conditions based on the abundance metrics                                                                                                                                                                  |     |  11,646,100 |     | 3-5-2042  |     | Issued/Allowed: CA  
 Pending: CN, EU, HK |

171

| Patent 
 Family |     | Patent Name                                                                                                                        |     | Summary                                                                                                                                                                                                                                                                                                                                                                                                        |     | U.S. Patent 
      Number |     | U.S. Exp. 
 Date      |     | Other Jurisdictions                                                                           |
| P03    |     | Sequencing Output Determination and Analysis with Target-Associated Molecules in Quantification Associated with Biological Targets |     | A method and/or system for generating a set of target-associated molecules (e.g., spike-in molecules) associated with biological                                                                                                                                                                                                                                                                               
 targets; generating spike-in mixtures based on processing the set of target-associated molecules with samples including the biological targets; performing Sanger sequencing operations on the spike-in mixtures; determining abundance metrics based on chromatogram-related outputs from the Sanger sequencing operations; and/or facilitating characterization of medical conditions based on the abundance 
 metrics.                                                                                                                                                                                                                                                                                                                                                                                                       |     |  11,430,543 |     | 6-24-2041 |     | Issued/Allowed: AU, BE, BZ, CA, EU, FR,                                                       
 DE, IL, IT, JP, KR, NL, SG, EP, UK   Pending: CN, HK, IN, SG                                  |
| P04    |     | Quality Control Templates for Ensuring Validity of Sequencing-Based Assays                                                         |     | A method and/or system for generating a set of quality control template (QCT) molecules; determining a set of QCT sequence read clusters based on the set of QCT molecules,                                                                                                                                                                                                                                    
 such as based on variation regions of the set of QCT molecules; and based on the set of QCT sequence read clusters, determining a sequencing-related parameter, such as a contamination parameter and/or molecule count parameter, associated with the at                                                                                                                                                      
 least one of sequencing library preparation and