Company: BLLN
Filing Date: 2025-08-11
Form Type: DRS/A
Source: 0000950123-25-007483
Chunk: 219

Company: BillionToOne, Inc.
Filing Date: 2025-08-11
Form: DRS/A
Chunk 219
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 sequencing operations on the spike-in mixtures; determining abundance metrics based on chromatogram-related outputs from the Sanger sequencing operations; and/or facilitating characterization of medical conditions based on the abundance 
 metrics.                                                                                                                                                                                                                                                                                                                                                                                                       |     |  11,430,543 |     | 6-24-2041 |     | Issued/Allowed: AU, BE, BZ, CA, EU, FR,                                                       
 DE, IL, IT, JP, KR, NL, SG, EP, UK   Pending: CN, HK, IN, SG                                  |
| P04    |     | Quality Control Templates for Ensuring Validity of Sequencing-Based Assays                                                         |     | A method and/or system for generating a set of quality control template (QCT) molecules; determining a set of QCT sequence read clusters based on the set of QCT molecules,                                                                                                                                                                                                                                    
 such as based on variation regions of the set of QCT molecules; and based on the set of QCT sequence read clusters, determining a sequencing-related parameter, such as a contamination parameter and/or molecule count parameter, associated with the at                                                                                                                                                      
 least one of sequencing library preparation and sequencing.                                                                                                                                                                                                                                                                                                                                                    |     |  11,629,381 |     | 2-17-2042 |     | Issued/Allowed: AU, AT, BE, BZ, CN, CY,                                                       
 DN, EU, FN, FR, DE, GR, HK, IN, IR, IL, IT, JP, KR, LE, NL, NO, PT, EP, SE, CH, UK   Pending: 
 CA, HK, JP, SG                                                                                |

166

| Patent 
 Family |     | Patent Name                                                                        |     | Summary                                                                                                                                                                                                                                                |     | U.S. Patent 
      Number |     | U.S. Exp. 
 Date      |     | Other Jurisdictions |
| P05    |     | Homologous Genomic Regions for Characterization Associated with Biological Targets |     | A method and/or system for generating a co-amplified mixture based on co-amplifying a set of                                                                                                                                                           
 nucleic acid molecules (e.g., cell-free nucleic acids, etc.) from the biological sample, wherein the set of nucleic acid molecules includes a genomic region of interest associated with the medical condition; and a homologous native genomic region 
 with partial sequence similarity to the genomic region of interest; sequencing the co-amplified mixture; determining an abundance metric for the genomic region of interest