Company: BLLN
Filing Date: 2025-08-11
Form Type: DRS/A
Source: 0000950123-25-007483
Chunk: 196

Company: BillionToOne, Inc.
Filing Date: 2025-08-11
Form: DRS/A
Chunk 196
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 RhD-CE-Dhybrid genes, which are prevalent in Black (45%) and Asian (>10%) populations. Other fetal RhD NIPTs are limited in their ability to detect these genetic complexities. UNITY Fetal RhD NIPT addresses these limitations through our revolutionary smNGS platform. Since launching, UNITY Fetal RhD NIPT has been ordered for over 150,000 patients, 53with data published in peer-reviewed studies demonstrating 100% concordance with neonatal outcomes and sensitivity/specificity of >99.9%. Traditional Workflow UNITY Fetal RhD NIPT Fetal RhD antigen status is often unknown without invasive procedure ALL RhD- mothers receive Rh(D) immune globulin Fetal D-antigen presence / absence determined as early as 9 weeks 40% fetal RhD not detected: Rho(D) immune globulin not indicated UNITY Fetal Antigen NIPT Alloimmunization occurs when a pregnant individual’s immune system produces antibodies against fetal red blood cell (RBC) antigens. This can happen when a RhD negative pregnant mother carrying an RhD positive

| 51 |     | Wynn, J., Rego, S., Fang, J., Alford, B., Carter, R., & Hoskovec, J. (2024). Performance Characteristics of a Next Generation Sequencing-Based cfDNA Assay for Common Aneuploidies in a General Risk Population. Annals 
 of Gynecology and Obstetrics Research, 7(1), 1027.                                                                                                                                                                      |

| 52 |     | Percentage based on an internal validation report we issued in January 2025 conducted by an independent third party lab. |

| 53 |     | Rego, S., Balogun, O. A., Emanuel, K., Overcash, R., Gonzalez, J. M., Denomme, G. A., Hoskovec, J., King, H., Wilson, A., Wynn, J., & Moise, K. J. Jr. (2024). Cell-free DNA analysis for the determination of fetal 
 red blood cell antigen genotype in individuals with alloimmunized pregnancies. Obstetrics & Gynecology, 144(4), 436-443.                                                                                             |

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fetus does not receive RhIG. This can also happen with other rare RBC antigens are present, such as Kell, especially due to a blood transfusion prior to the pregnancy. This can