Company: APM
Filing Date: 2025-12-05
Form Type: 424B5
Source: 0001213900-25-118752
Chunk: 303

Company: Aptorum Group Ltd
Filing Date: 2025-12-05
Form: 424B5
Chunk 303
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 subgroup. miRNA pairs and classifiers effectively differentiated defined subgroups. In line with DiamiR’s results in other studies, sex-stratified analysis yielded higher AUC values for females and males as compared to the combined group. The data supports the development of CogniMIR ®miRNA biomarkers for better characterization of MCI/AD patients and understanding of AD-associated processes. Further, combining CogniMIR ®miRNAs with other factors, such as amyloid and APOE, may enhance a biomarker algorithm.

| Separation of different subgroups using one pair, two pairs and three pairs of miRNAs. AUC: the area under the ROC curve. |

(Alzheimer’s Association
International Conference 2023, Amsterdam, Netherlands, poster presentation)

On June 19, 2024, DiamiR
announced acceptance of its abstract titled “Classifier algorithms to characterize various stages of Alzheimer’s disease based
on analysis of circulating brain-enriched and inflammation-associated microRNAs” for poster presentation at the 2024 International
Alzheimer’s Association Conference (AAIC) on July 29, 2024 (https://www.prnewswire.com/news-releases/DiamiR-biosciences-announces-poster- presentation-at- 2024-alzheimers-association-international-conference-aaic-302176323.html). On July 29, 2024, on behalf of DiamiR,
Dr. Kumar reported the comparative analysis of 24 microRNAs comprising CogniMIR panel in 200 plasma samples with
DiamiR’s Generation 1 and Generation 2 software algorithms towards distinguishing cognitively unimpaired, MCI and AD cohorts. The
plasma samples for this study were provided by Penn Alzheimer’s Disease Research Center, University of Pennsylvania and the analysis
was conducted at DiamiR laboratory between 2022 and 2024. This study builds upon results reported by DiamiR at the AAIC 20023 and described
below (see Business section, Characterizing Heterogeneous Groups of Patients with MCI, pre-MCI, and Dementia). miRNA classifiers based
on generation 2 algorithm differentiated between cognitively unimpaired, MCI and AD cohorts.

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HEALTHY BRAIN AGING

Using plasma samples collected
at the New York Blood Center from cognitively unimpaired individuals 26-35, 36-