Company: BIAF
Filing Date: 2025-10-09
Form Type: 424B5
Source: 0001493152-25-017591
Chunk: 1

Company: bioAffinity Technologies, Inc.
Filing Date: 2025-10-09
Form: 424B5
Chunk 1
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 | 1,800,000.00 |
| Placement agent fees(1)          |     | $         | 0.20 |     | $     |   144,000.00 |
| Proceeds, before expenses, to us |     | $         | 2.30 |     | $     | 1,656,000.00 |

| (1) | In                                                                                                                      
 addition, we have agreed to pay for certain expenses of WallachBeth up to $75,000. See “Plan of Distribution” beginning 
 on page S-8 of this prospectus supplement for additional information regarding compensation payable to WallachBeth.     |

Delivery of the shares of our common stock is expected to be made on or about October 9, 2025.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or passed upon the adequacy or accuracy of this prospectus supplement or the accompanying base prospectus. Any representation to the contrary is a criminal offense.

<div align='center'>WallachBeth Capital, LLC

The date of this prospectus supplement is October 8, 2025</div>

<div align='center'>TABLE OF CONTENTS

Prospectus Supplement</div>

| ABOUT THIS PROSPECTUS SUPPLEMENT                     | S-ii  |
| CAUTIONARY NOTE REGARDING FORWARD LOOKING STATEMENTS | S-iii |
| PROSPECTUS SUPPLEMENT SUMMARY                        | S-1   |
| THE OFFERING                                         | S-3   |
| RISK FACTORS                                         | S-4   |
| USE OF PROCEEDS                                      | S-5   |
| DIVIDEND POLICY                                      | S-5   |
| CAPITALIZATION                                       | S-6   |
| DILUTION                                             | S-7   |
| PLAN OF DISTRIBUTION                                 | S-8   |
| LEGAL MATTERS                                        | S-11  |
| EXPERTS                                              | S-11  |
| WHERE YOU CAN FIND MORE INFORMATION                  | S-11  |
| INCORPORATION OF CERTAIN INFORMATION BY REFERENCE    | S-12  |

<div align='center'>Prospectus</div>

|                                                   | Page |
| About this Prospectus                             |   ii |
| Prospectus Summary                                |    1 |
| Risk Factors                                      |    6 |
| Forward-Looking Statements                        |   33 |
| Use of Proceeds                                   |   34 |
|