Company: BLLN
Filing Date: 2025-10-07
Form Type: S-1
Source: 0001193125-25-233697
Chunk: 130

Company: BillionToOne, Inc.
Filing Date: 2025-10-07
Form: S-1
Chunk 130
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) the filing and effectiveness of our Post-IPO Certificate of Incorporation, all of which will occur immediately prior to the completion of this offering; and               |

| • |     | on a pro forma as adjusted basis to reflect (i) the pro forma adjustments set forth above and (ii) the issuance                                                                                               
 and sale of   shares of Class A common stock in this offering at an assumed initial public offering price of $   per share, the midpoint of the estimated offering price range set forth on the cover page of 
 this prospectus, after deducting underwriting discounts and commissions and estimated offering expenses payable by us.                                                                                        |

The pro forma and pro forma as adjusted information below is illustrative only, and our capitalization following the completion of this offering will be adjusted based on the actual initial public offering price and other terms of this offering determined at pricing. You should read the information in this table together with the sections titled “Management’s discussion and analysis of financial condition and results of operations” and “Description of capital stock” and our financial statements and the related notes included elsewhere in this prospectus.

|                                                                                                                                                                                       |     | As of June 30, 2025 |          |   |     |       |     |                |
|:--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|:----|:--------------------|---------:|:--|:----|:------|:----|:---------------|
| (in thousands, except for share and per share amounts)                                                                                                                                |     | Actual              |          |   |     | Pro   
 forma |     | Pro forma      
 as adjusted(1) |
| Cash and cash equivalents                                                                                                                                                             |     | $                   |  188,958 |   |     | $     |     | $              |
| Long-term debt                                                                                                                                                                        |     |                     |   52,072 |   |     |       |     |                |
| Redeemable convertible preferred stock, par value $0.00001 per share; 29,544,989 shares authorized,                                                                                   
 29,084,235 shares issued and outstanding, actual; no shares authorized, issued and outstanding, pro forma and pro forma as adjusted                                                   |     | $                   |  419,409 |   |     | $     |     | $              |
| Stockholders’ (deficit) equity:                                                                                                                                                       |     |                     |          |   |     |       |     |                |
| Preferred stock, par value $0.