Company: BUDZ
Filing Date: 2025-03-31
Form Type: 10-K
Source: 0001096906-25-000350
Chunk: 12

Company: WEED, INC.
Filing Date: 2025-03-31
Form: 10-K
Item: Item 1
Chunk 12
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 in the industry and our unique validation protocols, will allow us to measure up favorably when compared
to our competition.

Next Generation Sequencing

Next-generation sequencing
(NGS), introduced nearly ten years ago, is the catch-all term used to describe several sequencing technologies including:

    ●
    Illumina (Solexa) sequencing

    ●
    Roche 454 sequencing

    ●
    Ion torrent: Proton / PGM sequencing

    ●
    SOLiD sequencing

These recent sequencing
technologies allow scientists to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing, and as
such, have greatly expanded the study of genomics and molecular biology. Numerous laboratories within the Cannabis community are currently
employing this technology.

Colorado State Universities

To the best of our knowledge,
Colorado State Universities have conducted Cannabis Genomic Research Initiatives, which is currently seeking to describe the Cannabis
genome. The data generated through this effort is provided through the public domain to growers in an effort to stimulate the production
of new, high-value stains of Cannabis.

Anandia Labs

Anandia Labs is conducting
work in the area of Cannabis genomics based on sequence work which was completed in 2011. The sequencing work conducted was based on “next
generation sequencing” technology and resulted in the generation of tens-of-thousands of DNA segments that have yet to be completely
and correctly reassembled. Much of the sequence data that was generated through their sequencing efforts has been placed into the public
domain and shared with other laboratories. In some instances, the data has been found to be less than accurate.

Phylos Biosciences

Phylos Biosciences is currently
using DNA-based genetic fingerprinting to establish relationships between strains and to assist in the development of phenotypic databases
to accelerate traditional breeding programs. Phylos Biosciences has a primary goal of bringing clarity to the Cannabis market and promote
the generation of IP held by individual growers. To the best of our knowledge, Phylos Biosciences is not engaged in whole genome sequencing
and is not engaged in any genetic enhancements of the Cannabis strains. They simply supply genetic data to their customer base to
more effectively drive the traditional breeding process.

10

New West Genetics

New West Genetics aims to
improve and develop industrial hemp as a viable crop for the United States. New West Genetics seeks to exploit the diverse end uses of
hemp and optimize the genetics of hemp to