Company: BIAF
Filing Date: 2025-10-09
Form Type: 424B5
Source: 0001493152-25-017591
Chunk: 0

Company: bioAffinity Technologies, Inc.
Filing Date: 2025-10-09
Form: 424B5
Chunk 0
---
Filed Pursuant to Rule 424(b)(5)

Registration No. 333-275608

Prospectus Supplement

(to Prospectus dated November 27, 2023)

<div align='center'>720,000 Shares of Common Stock

bioAffinity Technologies, Inc.</div>

We are offering 720,000 shares of our common stock, $0.007 par value per share (the “common stock”), directly to institutional investors pursuant to this prospectus supplement and the accompanying prospectus. The per share offering price of the shares is $2.50.

Our common stock is listed on The Nasdaq Capital Market under the symbol “BIAF.” On October 7, 2025, the last reported sale price of our common stock on The Nasdaq Capital Market was $2.45 per share.

As of the date of this prospectus supplement, the aggregate market value of our outstanding common stock held by non-affiliates is approximately $38,804,591, which is calculated based on 3,692,159 shares of our outstanding common stock held by non-affiliates and a price of $10.51 per share, the closing price of our common stock on September 15, 2025, which is the highest closing sale price of our common stock on the Nasdaq Capital Market within the prior 60 days of this prospectus supplement. During the prior twelve-calendar-month period that ends on and includes the date hereof, we have offered and sold $4,849,353 of shares of our common stock pursuant to General Instruction I.B.6 to Form S-3.

Investing in our common stock involves a high degree of risk. Before buying any of our securities, you should carefully read “ Risk Factors” on page S-4 of this prospectus supplement, and under similar headings in the other documents that are incorporated by reference into this prospectus supplement and the accompanying prospectus.

We have engaged WallachBeth Capital, LLC (“WallachBeth”) to act as our exclusive placement agent in connection with this offering to use its reasonable best efforts to place the shares of common stock offered by this prospectus supplement. We have agreed to pay WallachBeth the fees set forth in the table below.

|                                  |     | Per Share |      |     | Total |              |
|:---------------------------------|:----|:----------|-----:|:----|:------|-------------:|
| Offering price                   |     | $         | 2.50 |     | $