Company: GANX
Filing Date: 2025-07-15
Form Type: 424B5
Source: 0001104659-25-068103
Chunk: 37

Company: Gain Therapeutics, Inc.
Filing Date: 2025-07-15
Form: 424B5
Chunk 37
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Risk Factors” contained in the applicable prospectus supplement and any related free writing prospectus, and under similar headings in the other documents that are incorporated by reference into this prospectus as described on page 7of this prospectus.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or determined if this prospectus is truthful or complete. Any representation to the contrary is a criminal offense.

<div align='center'>The date of this prospectus is June 1, 2022.</div>

TABLE OF CONTENTS

TABLE OF CONTENTS

| ​ | ABOUT THIS PROSPECTUS                             | ​ | ​ | ​ | ​ |  i | ​ | ​ |
| ​ | SUMMARY                                           | ​ | ​ | ​ | ​ |  1 | ​ | ​ |
| ​ | RISK FACTORS                                      | ​ | ​ | ​ | ​ |  7 | ​ | ​ |
| ​ | SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS | ​ | ​ | ​ | ​ |  8 | ​ | ​ |
| ​ | USE OF PROCEEDS                                   | ​ | ​ | ​ | ​ | 10 | ​ | ​ |
| ​ | DESCRIPTION OF CAPITAL STOCK                      | ​ | ​ | ​ | ​ | 11 | ​ | ​ |
| ​ | DESCRIPTION OF DEBT SECURITIES                    | ​ | ​ | ​ | ​ | 15 | ​ | ​ |
| ​ | DESCRIPTION OF WARRANTS                           | ​ | ​ | ​ | ​ | 22 | ​ | ​ |
| ​ | LEGAL OWNERSHIP OF SECURITIES                     | ​ | ​ | ​ | ​ | 24 | ​ | ​ |
| ​ | PLAN OF DISTRIBUTION                              | ​ | ​ | ​ | ​ | 27 | ​ | ​ |
| ​ | LEGAL MATTERS                                     | ​ | ​ | ​ | ​ | 29 | ​ | ​ |
| ​ | EXPERTS                                           | ​ | ​ | ​ | ​ | 29 | ​ | ​ |
| ​ | WHERE YOU CAN FIND MORE INFORMATION               | ​ | ​ | ​ | ​ | 30 | ​ | ​ |
| ​ | INCORPORATION OF CERTAIN INFORMATION BY REFERENCE | ​ | ​ | ​ | ​ | 31 | ​ | ​ |

TABLE OF CONTENTS

### ABOUT THIS PROSPECTUS
This prospectus is a part of a registration statement on Form S-3 that we filed with the Securities and