Company: GAINI
Filing Date: 2025-11-06
Form Type: 424B2
Source: 0001193125-25-269767
Chunk: 50

Company: GLADSTONE INVESTMENT CORPORATION\DE
Filing Date: 2025-11-06
Form: 424B2
Chunk 50
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| • |     | an insurance company; |

| • |     | a tax-exempt organization, private college and university endowments, retirement plan, individual retirement account or tax deferred account; |

| • |     | subject to the alternative minimum tax provisions of the Code; |

| • |     | holding the Notes as part of a hedge, straddle, conversion, integrated or other risk reduction or constructive sale transaction; |

| • |     | holding the Notes through a partnership or other pass-through entity; |

| • |     | a “United States person” (within the meaning of the Code) whose “functional currency” is not the U.S. dollar; or |

| • |     | a U.S. expatriate or former long-term resident. |

In addition, this discussion does not reflect state, local or non-U.S.tax consequences that may apply to you based on your particular circumstances and residence. This discussion also does not address any U.S. federal tax consequences, such as the estate tax or gift tax, other than U.S. federal income tax consequences. You are urged to consult your tax advisors to determine the tax consequences particular to your situation, including any applicable state, local or foreign income and other tax consequences, which may result from your acquisition, ownership or disposition of the Notes. As used herein, “U.S. holder” means a beneficial owner of the Notes that is, for U.S. federal income tax purposes:

| • |     | an individual who is a citizen or resident of the United States; |

| • |     | a corporation (or other entity treated as a corporation for U.S. federal income tax purposes) created or organized in or under the laws of the United States, any state thereof or the District of Columbia; |

S-3 4

| • |     | an estate, the income of which is subject to U.S. federal income tax regardless of its source; or |

| • |     | a trust that (1) is subject to the primary supervision of a U.S. court and the control of one or more “United States persons” (within the meaning of the Code) that have the authority to control all substantial decisions of the trust, or (2) has a valid election in effect under applicable Treasury Regulations to be treated as a United States person. |

For purposes of this discussion, “non-U.S.holder” means a beneficial owner of the Notes that is neither a U.S. holder nor an entity treated as a partnership