Company: TEM
Filing Date: 2025-08-27
Form Type: 424B7
Source: 0001193125-25-189854
Chunk: 11

Company: Tempus AI, Inc.
Filing Date: 2025-08-27
Form: 424B7
Chunk 11
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 of Proceeds.”

Nasdaq Global Select Market Listing

Our Class A common stock is listed on the Nasdaq Global Select Market under the symbol “TEM.”

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RISK FACTORS

Investing in our Class A common stock involves a high degree of risk. Before deciding whether to invest in our Class A common stock,
you should consider carefully the risks and uncertainties described under the section entitled “RiskFactors” contained in our most recent Annual Report on Form 10-K and subsequent Quarterly
Reports on Form 10-Q or Current Reports on Form 8-K, as well as any amendments thereto reflected in subsequent filings with the SEC, together with other information in
this prospectus supplement and the accompanying prospectus and the documents incorporated by reference in this prospectus supplement and the accompanying prospectus. See the sections titled “Where You Can Find More Information” and
“Incorporation of Certain Informationby Reference.” The risks described in these documents are not the only ones we face, but those that we consider to be material. There may be other unknown or unpredictable economic, business,
competitive, regulatory or other factors that could have material adverse effects on our future results. Past financial performance may not be a reliable indicator of future performance, and historical trends should not be used to anticipate results
or trends in future periods. If any of these risks actually occurs, our business, financial condition, results of operations or cash flow could be seriously harmed. This could cause the trading price of our Class A common stock to decline,
resulting in a loss of all or part of your investment. Please also carefully read the section entitled “Special NoteRegarding Forward-Looking Statements.”

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USE OF PROCEEDS

All of the shares of Class A common stock being offered hereby are being sold by the selling stockholders. The selling stockholders
referenced in this prospectus supplement will receive all of the net proceeds from the sales of shares of Class A common stock. We will not receive any proceeds from the sale of Class A common stock by the selling stockholders.

We, and not the selling stockholders, will pay the costs, expenses and fees in connection with the registration of the shares of Class A
common stock covered by this prospectus supplement, but the selling stockholders will pay all discounts, commissions or brokers’ fees or fees of similar securities industry professionals and transfer taxes, if any, attributable to sales of
such shares of Class A common stock.

S-8

SELLING STOCKHOLDERS Up to