Company: GAINI
Filing Date: 2025-11-20
Form Type: 40-17G
Source: 0001193125-25-289175
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Company: GLADSTONE INVESTMENT CORPORATION\DE
Filing Date: 2025-11-20
Form: 40-17G
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November 20, 2025 Office of Registration and Reports Securities and Exchange Commission 100 F Street, NE Washington, DC 20549 RE: Gladstone Investment Corporation Fidelity Bond Ladies and Gentlemen: On behalf of Gladstone Investment Corporation (the “Company”), I enclose the following documents for filing pursuant to Rule 17g-1of the Investment Company Act of 1940, as amended (the “1940 Act”):

| 1. | A copy of the Company’s Investment Company Bond No. 106628401, in the amount of $3,000,000,                                                            
 effective as of November 25, 2025, covering the Company, and its subsidiaries (the “Bond”). The Bond was received by the Company on November 12, 2025. |

The period for which premiums have been paid on behalf of the Company is November 25, 2025 to November 25, 2026. The Company’s premium is $9,972. Thank you for your assistance. Should you have any questions, please feel free to call me at 703-287-5872.

| Sincerely,                       |
| GLADSTONE INVESTMENT CORPORATION |
| /s/ John A. Dellafiora Jr.       |
| John A. Dellafiora Jr.           |
| Chief Compliance Officer         |

| Luke W McCarty PO Box 64094                   
 St. Paul, MN 55102-0094 Phone: (646) 509-0520 
 Email: LMCCARTY@travelers.com                 |

November 12, 2025 Justin Salazar AON RISK SERVS CENTRAL 200 E RANDOLPH ST FL 9 CHICAGO, IL 60601

| RE: |     | Insured Name:           |     |       GLADSTONE INVESTMENT CORPORATION |
|     |     | Binder Type:            |     |                           Premium Only |
|     |     | Product:                |     |                INVESTMENT COMPANY BOND |
|     |     |                         |     |                     IVBB-15001 (01-16) |
|     |     | Bond Number:            |     |                              106628401 |
|     |     | Bond Period:            |     | November 25, 2025 to November 25, 2026 |
|     |     | Binder Expiration Date: |     |                      December 12, 2025 |

Dear Justin Salazar