Company: BOLT
Filing Date: 2025-03-24
Form Type: S-8
Source: 0000950170-25-043900
Chunk: 1

Company: Bolt Biotherapeutics, Inc.
Filing Date: 2025-03-24
Form: S-8
Chunk 1
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 to Section 7(a)(2)(B) of the Securities Act.☒

<div align='center'>EXPLANATORY NOTE</div>

Bolt Biotherapeutics, Inc. (the “Registrant”) is filing this Registration Statement on Form S-8 for the purpose of registering an additional (a) 1,916,984 shares of its common stock, par value $0.00001 per share (the “Common Stock”), issuable to eligible persons under the 2021 Equity Incentive Plan, which Common Stock is in addition to the shares of Common Stock registered on the Registrant’s registration statement on Form S-8s filed onFebruary 5, 2021 (File No. 333-252815),March 30, 2022 (File No. 333-263987),March 29, 2023 (File No. 333-270938), andMarch 21, 2024 (File No. 333-278141), (the “Prior Form S-8s”), and (b) 383,396 shares of Common Stock issuable to eligible persons under the 2021 Employee Stock Purchase Plan, which Common Stock is in addition to the shares of Common Stock registered on the Prior Form S-8s.

# PART II
| ITEM 3. | INCORPORATION OF CERTAIN DOCUMENTS BY REFERENCE |

Pursuant to General Instruction E to Form S-8, the contents of the Prior Form S-8s are incorporated by reference herein.

The following documents filed by the Registrant with the Commission are incorporated by reference into this Registration Statement:

(a) The Registrant’sAnnual Report on Form 10-Kfor the fiscal year ended December 31, 2024 filed on March 24, 2025, which contains audited financial statements for the Registrant’s latest fiscal year.

(b) The description of the Common Stock which is contained in a registration statement onForm 8-Afiled on February 2, 2021 (File No. 001-39988) under the Securities Exchange Act of 1934, as amended (the “Exchange Act”), including any amendment or report filed for the purpose of updating such description.

(c) All other reports and documents subsequently filed by the Registrant pursuant to Sections 13(a), 13(c), 14 and 15(d) of the Exchange Act (other than Current Reports furnished under Item 2.02 or Item 7.01 of Form