Company: GAINI
Filing Date: 2025-11-06
Form Type: 424B2
Source: 0001193125-25-269767
Chunk: 12

Company: GLADSTONE INVESTMENT CORPORATION\DE
Filing Date: 2025-11-06
Form: 424B2
Chunk 12
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 contained in Section 18(a)(1)(B) as modified by Section 61(a)(2) of the 1940 Act, in order to maintain such BDC’s status as a RIC under Subchapter M of the Code. |

| Events of default | The term “Event of Default” in respect of the Notes means any of the following: |

| • |     | We do not pay the principal of any Note when due and payable at maturity; |

| • |     | We do not pay interest on any Note when due and payable, and such default is not cured within 30 days of its due date; |

| • |     | We remain in breach of any other covenant in respect of the Notes for 60 days after we receive a written notice of default stating we are in breach (the notice must be sent by either the trustee or holders of at least 25% of the principal amount of the outstanding Notes); |

| • |     | We file for bankruptcy or certain other events of bankruptcy, insolvency or reorganization occur and remain undischarged or unstayed for a period of 90 days; or |

| • |     | On the last business day of each of twenty-four consecutive calendar months, the Notes have an asset coverage, as defined in the 1940 Act, of less than 100%. |

| Use of Proceeds | We estimate that the net proceeds we receive from the sale of the $60.0 million aggregate principal amount of Notes in this offering will be approximately $58.7 million after deducting estimated offering expenses of approximately $0.4 million payable by us. |

| Use of Proceeds 
 S-14.           |

S-7

| Global Clearance and Settlement Procedures | Interests in the Notes will trade in DTC’s Same Day Funds Settlement System, and any permitted secondary market trading activity in such Notes will, therefore, be required by DTC to be settled in immediately available funds. None of us, the Trustee or the paying agent will have any responsibility for the performance by DTC or its participants or indirect participants of their respective obligations under the rules and procedures governing their operations. |

| Governing Law | The Notes and the indenture will be governed by and construed in accordance with the laws of the State of New York. |

| Risk Factors | See “Supplementary Risk Factors” on pageS-9of this prospectus supplement, “Risk Factors” beginning on page 5 of the accompanying prospectus and under similar heading in