Company: QTIWW
Filing Date: 2025-12-31
Form Type: 424B3
Source: 0001628280-25-059235
Chunk: 391

Company: QT IMAGING HOLDINGS, INC.
Filing Date: 2025-12-31
Form: 424B3
Chunk 391
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 |         — |     |        |   — |     |            | -5,000,000 |     |             |           — |     |       |  -5,000,000 |
| Issuance of warrants related to private placements                  |     |              |     |         — |     |        |   — |     |            |    380,511 |     |             |           — |     |       |     380,511 |
| Exercise of warrants related to public placements(1)                |     |              |     |    77,039 |     |        |   8 |     |            |    531,614 |     |             |           — |     |       |     531,622 |
| Exercise of incentive stock options(1)                              |     |              |     |    33,333 |     |        |   3 |     |            |     74,836 |     |             |           — |     |       |      74,839 |
| Stock-based compensation                                            |     |              |     |         — |     |        |   — |     |            |    518,721 |     |             |           — |     |       |     518,721 |
| Net loss                                                            |     |              |     |         — |     |        |   — |     |            |          — |     |             | -19,694,921 |     |       | -19,694,921 |
| Balance, September 30, 2025(1)                                      |     |              |     | 9,666,567 |     | $      | 967 |     | $          | 42,558,037 |     | $           | -51,635,448 |     | $     |  -9,076,444 |

<div align='center'>F-51</div>

#### QT IMAGING HOLDINGS, INC.
<div align='center'>Condensed Consolidated Statements of Stockholders’ Deficit

For the Three and Nine Months Ended September 30, 2025 and 2024

(Unaudited)</div>

|                                                                            |     | Common Stock 
 Shares       |            |           |     | Amount |      |     | Additional 
 Paid-in    
 Capital    |             |     | Accumulated 
 Deficit     |             |     | Total |             |
|:---------------------------------------------------------------------------|:----|:-------------|-----------:|