Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 189

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 189
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 inform high-value clinical decisions, a sensing element
inside the body to take direct measurements of the analyte of interest is of critical importance.

Current CGM sensors typically
have a small needle coated with a sensing chemistry which is placed through the skin so the needle can take measurements in the interstitial
space of a patient. These needles, however small, elicits a “foreign body response” within the patient as the body’s
natural defense mechanism against objects that are recognized as foreign. The foreign body response begins to encapsulate the sensing
needle of these CGMs in scar tissue and renders the data from these sensors unreliable within a matter of days. This is the major
reason the longevity of these CGMs is limited to approximately 7-14 days. A longer-term 12-month solution where a sensor is
surgically implanted into a patient’s body is available as well, however the sensor requires a surgical implantation with sutures
to close the surgical wound. This approach has a limitation of a much more invasive deployment procedure and associated higher cost.

<div align='center'>106</div>

All data is from the manufacturer website. The products provided in this chart are:

| ● | Dexcom G6 |

| ● | Abbott Freestyle Libre 2 |

| ● | Medtronic Guardian Connect |

| ● | Senseonics Eversense E3 |

Profusa’s CGM Product (Lumee Glucose) is not approved in the United States. The > 270 days usage time is based on the data acquired through Profusa’s glucose program clinical study.

Profusa’s Technology

It is Profusa’s belief,
based on an understanding of the biological response to current available sensors, that a viable solution that could provide data for
clinical utility and wide-scale adoptions, needs to have the following three key characteristics: direct measurement of the body’s
chemistry to enable accuracy, ease of deployment of the platform and long-term functionality to encourage adoption by a large population,
and low cost to breakdown the economic barrier for adoption by those who can benefit from this data stream.

Profusa’s sensor platform
has the potential to deliver on these requirements and are composed of the following components:

| ● | Hydrogel Sensor |

Profusa’s passive sensor is composed
of a class of materials called hydrogels, which are similar to the material from which modern contact lenses are made. The sensor is designed
to overcome the effect of the foreign body response and thus has been demonstrated to be able to collect biochemical data for up