Company: RVRC
Filing Date: 2025-12-12
Form Type: S-1/A
Source: 0001213900-25-121070
Chunk: 85

Company: Revium Rx.
Filing Date: 2025-12-12
Form: S-1/A
Chunk 85
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us infections; Oliver Goldmanna,, Ahuva Cern, Mathias Mueskenc, Manfred Rohdec, William Weissd, Yechezkel Barenholzb, Eva Medinaa https://doi.org/10.1016/j.jconrel.2019.11.007

| 2. | Murine                             
 Model – Neutropenic Lung Infection |

In a neutropenic lung infection model, female
CD-1 (ICR) mice (5–6 weeks old) were rendered neutropenic via cyclophosphamide (150 mg/kg and 100 mg/kg on days -4 and -1). Mice
were anesthetized and intranasally inoculated with ~5 × 10⁷ CFU of MRSA (UNT141-3). Treatment groups (n=5 per group) received
IV doses of Nano-Mupirocin (50 or 75 mg/kg), free mupirocin (same doses), or blank liposomes 2 hours post-inoculation. Additional control
groups were treated with vancomycin. Mice were euthanized at 24 hours, and lung bacterial loads were measured by plating organ homogenates
on selective agar. Nano-Mupirocin-treated groups showed up to 2.28 log₁₀ CFU reductions compared to free mupirocin at equivalent
doses.

<div align='center'>51</div>

Representative results from the neutropenic lung infection animal
model are shown below:

| Fig.                                                                                              
 2. Therapeutic effect of parenterally administered Nano-Mupirocin or free mupirocin against       
 MRSA in a neutropenic murine model of lung infection. (A) Schematic illustration of therapeutic   
 regimen in neutropenic mice. CD1 mice were rendered neutropenic and infected intranasally         
 with app. 5×107 CFU of MRSA strain UNT141-3 and treated with the indicated dose of                
 either Nano-Mupirocin, free mupirocin, Vancomycin or with blank nanoliposomes. Mice were          
 sacrificed at 24 h of infection and bacteria enumerated in the lungs. (B) Bacterial loads         
 in the lungs of the different groups of mice treated according to scheme depicted in (A)          
 at 24 h of infection. Each symbol represents the value for an individual animal (n=5). Horizontal 
 lines indicate the mean ± SD.