Company: BLLN
Filing Date: 2025-08-11
Form Type: DRS/A
Source: 0000950123-25-007483
Chunk: 11

Company: BillionToOne, Inc.
Filing Date: 2025-08-11
Form: DRS/A
Chunk 11
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. Why Go Public Now This offering represents a pivotal moment in our journey. The capital we raise will accelerate our research and development initiatives, expand our commercial footprint, and enable us to bring our life-changing diagnostics to more patients. But this offering is about more than capital. It is about setting the stage for our next phase of growth and impact. It is about building the foundation for a company that will stand among the most consequential healthcare innovators of our time. We are not building just another successful company; we are changing the very landscape of healthcare. Millions of patients whose lives will be transformed may never know our name, but they will benefit from what we have built. We invite you to join us in writing this next chapter of medical history. Sincerely, Oguzhan Atay, PhD Co-Founder & CEO

Glossary

As used in this prospectus, unless we state otherwise or the context otherwise requires:

ACOG: American College of Obstetricians and Gynecologists—the primary medical organization that sets the guidelines, practice advisories, and standard of
care for women’s healthcare

ASP: Average selling price—The average payment BillionToOne receives per test (inclusive of any non-payments)

Calling threshold: For early cancer detection tests, this is the specific cutoff value at which a test
result is classified as “positive” (cancer detected) rather than “negative” (no cancer detected)

CF: Cystic fibrosis—A genetic
disorder that can be detected by UNITY tests

cfDNA: Cell-free DNA—Extracellular DNA in blood plasma that is shed from all tissues into the
bloodstream. Due to its short half-life, cfDNA has a unique capability to provide a real-time snapshot of cellular turnover and can be used to diagnose and monitor disease

CNV: Copy number variant—A variation in gene copies that Northstar Select can detect

Control spike-in:A known quantity of an external molecule - such as synthetic RNA - added to a biological sample prior to measurement. This serves as an internal
reference to monitor and correct for technical variation, enabling more accurate quantification and comparison across samples, especially in high-throughput sequencing and related assays

ctDNA: Circulating tumor DNA—Cell-free DNA released from tumor cells, which can be used as a biomarker for various cancer-related diagnostics

Methylation: A biochemical process where a methyl group (a carbon atom bonded to three hydrogen atoms) is added to a molecule, such as DNA. This process can
change gene expression