Company: BLLN
Filing Date: 2025-10-07
Form Type: S-1
Source: 0001193125-25-233697
Chunk: 305

Company: BillionToOne, Inc.
Filing Date: 2025-10-07
Form: S-1
Chunk 305
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 to purchase additional shares of common stock from us; or |

| • |     | the average weekly trading volume of our common stock during the four calendar weeks preceding the filing of a notice on 
 Form 144 with respect to such sale.                                                                                      |

Sales under Rule 144 by our affiliates or persons selling shares on behalf of our affiliates are also subject to certain manner of sale provisions and notice requirements and to the availability of current public information about us. Rule 701 Rule 701 under the Securities Act, or Rule 701, generally allows a stockholder who was issued shares under a written compensatory plan or contract and who is not deemed to have been an affiliate of our company during the immediately preceding 90 days, to sell these shares in reliance on Rule 144, but without being required to comply with the public information, holding period, manner of sale limitations, volume limitation, or notice provisions of Rule 144. Rule 701 also permits affiliates of our company to sell their Rule 701 shares under Rule 144 without complying with the holding period requirements of Rule 144. All holders of Rule 701 shares, however, are required by that rule to wait until 90 days after the date of this prospectus before selling those shares under Rule 701, subject to the expiration of the lock-upagreements and market stand-offarrangements described below. Form S-8registration statements We intend to file one or more registration statements on Form S-8under the Securities Act with the SEC as soon as practicable after the time at which the registration statement of which this prospectus forms a part becomes effective to register the offer and sale of shares of our Class A common stock that are subject to outstanding stock options and Class A common stock issued or issuable under our employee benefit plans as defined in Form S-8.These registration statements will become effective immediately on filing. Shares covered by these registration statements will then be eligible for sale in the public markets, subject to vesting restrictions, any applicable lock-upagreements and/or market stand-offarrangements described below and Rule 144 limitations applicable to affiliates. Lock-upagreements and market stand-offarrangements We will agree that we will not, subject to certain exceptions, (i) offer, pledge, sell, contract to sell, sell any option or contract to purchase, purchase any option or contract to sell, grant any option, right, or warrant to purchase, hedge, lend, or otherwise transfer or dispose of, directly or indirectly, or submit to, or file with