Company: NCEL
Filing Date: 2025-05-16
Form Type: 20-F
Source: 0001213900-25-044868
Chunk: 190

Company: NewcelX Ltd.
Filing Date: 2025-05-16
Form: 20-F
Item: Item 4
Chunk 190
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, 90-min apart, in the NOR test: a sample (acquisition) trial during which
they were exposed to two identical objects, and a choice (retention) trial during which they were exposed to two different objects presented,
the familiar object (presented at the sample trial), and the novel object. They received 30 min before the sample trial i. p. injections
of vehicle (control group), of scopolamine, of scopolamine + donepezil (1 mg/kg) or of scopolamine + NLS-8 (50, 100 or 150 mg/kg). Scopolamine
was injected at 1.2 mg/kg. The results of this pre-clinical study showed that NLS-8 improved amnesia induced by scopolamine suggesting
that NLS-8 may improve memory and reduce cognitive symptoms of AD. These effects were significant at doses of 50 and 150 mg/kg and was
close to significant at the dose of 100 mg/kg.

NLS-11

NLS-11 (Benedin) (formerly
SCH-5472) is a norepinephrine and dopaminereuptake inhibitor and muscarinic M1, M2, M3 receptor antagonist. In
a preclinical study, 57BL/6 male mice, 8 groups (N = 16 mice/group), were subjected to the effect of NLS-11 (0.1, 0.5, 1 mg/kg) and compared
to those of vehicle and of donepezil (2 mg/kg). Long-term episodic memory was tested in the NOR test, using a 3-days interval between
the acquisition session (called sample trial) and the retention session (called choice trial). The results of the study showed NLS-11
improved the recognition of the familiar object, i. e. improved memory and suggest that NLS-11 induced a long-term memory improvement which
was significant and of the same extent to that induced by donepezil. At doses tested, NLS-11 did not reduce exploratory behavior, contrary
to donepezil, suggesting that NLS-11 may induce less side effect than donepezil.

NLS-12

NLS-12 (Oxafuramine) is a
norepinephrine and dopamine reuptake inhibitor and muscarinic M4 receptor antagonist, improved long-term episodic memory in
mice. Donepezil was used as