Company: BLLN
Filing Date: 2025-10-17
Form Type: S-1/A
Source: 0001193125-25-242632
Chunk: 244

Company: BillionToOne, Inc.
Filing Date: 2025-10-17
Form: S-1/A
Chunk 244
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ution tags associated                                                      
 with a relative concentration profile; generating a second set of molecules including dilution tags; generating a dilution tagged mixture; amplifying the dilution tagged targets; generating a modified dilution tagged mixture from the amplified 
 subsets; determining, for the biological sample, a count of the distinct molecules including the target sequence.                                                                                                                                   |     |  12,071,651 |     | 3-19-2041 |     | Issued/Allowed: CA  
 Pending: CN, EU, HK |
| P02    |     | Target-Associated Molecules for Characterization Associated with Biological Targets |     | A method and/or system for facilitating characterization of one or more conditions that can include: generating a set of target-associated molecules; generating a                                                                                  
 reference-associated set of molecules; facilitating generation of a spike-in mixture; determining abundance metrics based on an analysis of the spike-in mixture; and                                                                               
 facilitating the characterization of the conditions based on the abundance metrics                                                                                                                                                                  |     |  11,646,100 |     | 3-5-2042  |     | Issued/Allowed: CA  
 Pending: CN, EU, HK |

176

| Patent 
 Family |     | Patent Name                                                                                                                        |     | Summary                                                                                                                                                                                                                                                                                                                                                                                                        |     | U.S. Patent 
      Number |     | U.S. Exp. 
 Date      |     | Other Jurisdictions                                                                           |
| P03    |     | Sequencing Output Determination and Analysis with Target-Associated Molecules in Quantification Associated with Biological Targets |     | A method and/or system for generating a set of target-associated molecules (e.g., spike-in molecules) associated with biological                                                                                                                                                                                                                                                                               
 targets; generating spike-in mixtures based on processing the set of target-associated molecules with samples including the biological targets; performing Sanger sequencing operations on the spike-in mixtures; determining abundance metrics based on chromatogram-related outputs from the Sanger sequencing operations; and/or facilitating characterization of medical conditions based on the abundance 
 metrics.                                                                                                                                                                                                                                                                                                                                                                                                       |     |  11,430,543 |     | 6-24-2041 |     | Issued/Allowed: AU, BE, BZ, CA, EU, FR,                                                       
 DE, IL, IT, JP, KR, NL, SG, EP, UK   Pending: CN, HK, IN, SG                                  |
| P04    |     | Quality Control Templates for Ensuring Validity of Sequencing-Based Assays