Company: TEM
Filing Date: 2025-03-07
Form Type: 424B3
Source: 0001193125-25-049935
Chunk: 0

Company: Tempus AI, Inc.
Filing Date: 2025-03-07
Form: 424B3
Chunk 0
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Filed Pursuant to Rule 424(b)(3)
Registration No. 333-285186

PROSPECTUS

UP TO 4,843,136 SHARES OF CLASS A COMMON STOCK

This prospectus relates to the offer and sale from time to time (after the expiration of any applicable lock-up period described herein) by REALM IDx, Inc., a Delaware corporation, or its permitted transferees, which we refer to as the Seller or the selling stockholder, of up to 4,843,136 shares, or the Shares, of our
Class A common stock, par value $0.0001, or Class A common stock. The Shares were issued to the selling stockholder pursuant to a Securities Purchase Agreement dated November 4, 2024, or the Purchase Agreement, by and among us, the
Seller and the Seller’s ultimate parent, Konica Minolta, Inc., a Japanese corporation, as guarantor, as stock consideration in connection with our acquisition of Ambry Genetics Corporation, a Delaware corporation, or Ambry, as more fully
described below in “Prospectus Summary—Acquisition of Ambry Genetics Corporation.” We are registering the offer and sale of the Shares from time to time by the selling stockholder to satisfy the registration rights granted to the
selling stockholder pursuant to the Purchase Agreement.

The selling stockholder may offer, sell or distribute all or a
portion of the Shares publicly or through private transactions at prevailing market prices or at negotiated prices after the expiration of any applicable lock-up period described in this prospectus. We will
not receive any of the proceeds from such sales of the Shares. We will bear all costs, expenses and fees in connection with the registration of these Shares. The selling stockholder will bear all commissions and discounts, if any, attributable to
their sale of the Shares. See the section entitled “Plan of Distribution.”

Our Class A common stock is
listed on the Nasdaq Global Select Market under the symbol “TEM.” On March 6, 2025, the last reported sales price of our Class A common stock was $46.86 per share.

We are an “emerging growth company” as defined under U.S. federal securities laws and, as such, have elected to
comply with reduced public company reporting requirements. This prospectus complies with the requirements that apply to an issuer that is an emerging growth company.

Investing in our securities involves a high degree of risks. You should review carefully the risks and uncertainties