Company: BLLN
Filing Date: 2025-09-17
Form Type: DRS/A
Source: 0001193125-25-206347
Chunk: 401

Company: BillionToOne, Inc.
Filing Date: 2025-09-17
Form: DRS/A
Chunk 401
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 2025, the Company evaluated subsequent events through September 17, 2025, the date on which the interim financial statements were available to be issued. F-62

shares Class A common stock Preliminary prospectus

| J.P. Morgan |     | Piper Sandler          |     | Jefferies |     | William Blair |
| Stifel      |     | Wells Fargo Securities |     |           |     | BTIG          |

, 2025

Part II Information not required in prospectus Item 13. Other expenses of issuance and distribution The following table sets forth all costs and expenses, other than underwriting discounts and commissions, payable by us in connection with the sale of the Class A common stock being registered. All amounts shown are estimates except for the Securities and Exchange Commission (SEC), registration fee, the Financial Industry Regulatory Authority (FINRA), filing fee and the Nasdaq listing fee.

| Item                                     |     | Amount     
 paid or    
 to be paid |   |
| SEC registration fee                     |     |            | * |
| FINRA filing fee                         |     |            | * |
| Nasdaq listing fee                       |     |            | * |
| Printing and engraving expenses          |     |            | * |
| Legal fees and expenses                  |     |            | * |
| Accounting fees and expenses             |     |            | * |
| Blue sky qualification fees and expenses |     |            | * |
| Transfer Agent fees and expenses         |     |            | * |
| Miscellaneous expenses                   |     |            | * |
| Total                                    |     | $          | * |

| * |     | To be completed by amendment |

Item 14. Indemnification of directors and officers Section 145 of the DGCL authorizes a court to award, or a corporation’s Board of Directors to grant, indemnity to directors and officers under certain circumstances and subject to certain limitations. The terms of Section 145 of the DGCL are sufficiently broad to permit indemnification under certain circumstances for liabilities, including reimbursement of expenses incurred, arising under the Securities Act. As permitted by the DGCL, our Post-IPOCertificate of Incorporation and Post-IPOBylaws, as each will be in effect immediately prior to the completion of this offering, contain provisions relating to the limitation of liability and indemnification of our directors and officers. The Post-IPOCertificate of Incorporation will provide that our directors will not be personally liable to us or our stockholders for monetary damages for any breach