Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 190

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 190
---
 to 6-months
in the case of Lumee Oxygen, and up to 9-months based on the data acquired through Profusa’s glucose program clinical study. This
hydrogel scaffolding is soft and pliable and serves as the base material on which specific fluorescent molecules are bound. These fluorescent
molecules bind specifically to the analyte of interest and their fluorescent characteristics change depending on whether the analyte is
bound or unbound. The hydrogel sensors are devoid of any electronics or power source, and are simply composed of the hydrogel scaffolding
and the fluorescence sensing chemistry. Depending on the sensor type, Profusa may also imbed a reference chemistry for reference purposes
during data processing. The hydrogel sensor measures approximately 400 microns in diameter by 3 millimeters in length and is injected
subcutaneously via a hypodermic needle injection at a depth of 2 – 6 millimeters from the surface. Upon injection, the sensor
is designed to be integrated as a part of the tissue and is not noticeable. As this sensor is passive in nature, while the binding and
unbinding of the analyte of interest is constantly occurring, data from the sensor is not actively broadcasted outside of the body until
a reader (described below) is placed on the skin over the sensor.

<div align='center'>107</div>

By “decoupling the sensor
and the reading elements,” we believe that our approach has a few major advantages:

| 1. | The cost of the system is quite low as the sensors are low cost to manufacture and the more expensive 
 reader component can be reused through multiple sensor injections;                                    |

| 2. | The act of data acquisition is controlled by the user and action is secure; |

| 3. | The sensor deployment is a common hypodermic needle procedure that can be performed by healthcare professionals 
 across a variety of common settings; and                                                                        |

| 4. | The sensor functionality lifetime is limited by the stability of the fluorescence molecule and not by 
 the foreign body response, which translates into months of functionality.                             |

| ● | Reusable Reader |

An optical reader has been developed
to be worn on the skin above the sensor and is designed to interrogate and gather sensor signal for data processing. This optical reader
has a small wearable form factor dimensions of the oxygen reader and is worn on the skin via a double-sided medical grade adhesive. The
readers are rechargeable via conductive charging and