Company: APM
Filing Date: 2025-07-15
Form Type: DRS
Source: 0001213900-25-063906
Chunk: 330

Company: Aptorum Group Ltd
Filing Date: 2025-07-15
Form: DRS
Chunk 330
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 carriers are effectively separated from APOE4 non -carriersby select miRNA classifiers. Separation of APOE4 carriers from non -carriersis improved in subgroups stratified by sex. The best separation is observed within sex -stratifiedamyloid positive subgroup with AUC = 0.88 for males and AUC = 0.86 for females. Correlations between other measured parameters, including p -tau 181and NfL, have also been determined. The results generated in this study indicate that levels of cell -freemiRNA biomarker candidates have a strong potential to be used in combination with other AD markers and risk factors to better characterize preclinical AD patients. (15 thClinical Trials on Alzheimer’s Disease (CTAD), 2022, San Francisco, CA, poster presentation) CHARACTERIZING HETEROGENEOUS GROUPS OF PATIENTS WITH MCI, PRE -MCI, AND DEMENTIA In this study, CogniMIR ®miRNA biomarkers were analyzed in plasma samples of 200 study participants with the following clinical diagnoses at the time of blood draw: 76 cognitively unimpaired (CU), 85 MCI, and 39 AD. The plasma samples were collected at the Penn Alzheimer’s Disease Research Center (ADRC). Based on amyloid status (A β+/-), APOE genotype (APOE4 +/-), and sex, each sample was assigned to a specific subgroup. miRNA pairs and classifiers effectively differentiated defined subgroups. In line with DiamiR’s results in other studies, sex -stratifiedanalysis yielded higher AUC values for females and males as compared to the combined group. The data supports the

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development of CogniMIR ®miRNA biomarkers for better characterization of MCI/AD patients and understanding of AD -associatedprocesses. Further, combining CogniMIR ®miRNAs with other factors, such as amyloid and APOE, may enhance a biomarker algorithm.

| Separation of different subgroups using one pair, two pairs and three pairs of miRNAs. AUC: the area under the ROC curve. |

(Alzheimer’s Association International Conference 2023, Amsterdam, Netherlands, poster presentation) On June 19, 2024, DiamiR announced acceptance of its abstract titled “Classifier algorithms to characterize various stages of Alzheimer’s disease based on analysis of circulating brain -enrichedand inflammation -associatedmicroRN