Company: BLLN
Filing Date: 2025-10-17
Form Type: S-1/A
Source: 0001193125-25-242632
Chunk: 36

Company: BillionToOne, Inc.
Filing Date: 2025-10-17
Form: S-1/A
Chunk 36
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-6 Warrant into a warrant to purchase 9,660 shares 
 of our common stock immediately prior to the completion of this offering;           |

| • |     | the filing and effectiveness of our Post-IPO Certificate of Incorporation                                                                                                                                                                          
 immediately prior to the completion of this offering, which, among other things, will reclassify all outstanding shares of our common stock into an equal number of shares of Class A common stock (the Reclassification), and the adoption of our 
 amended and restated bylaws (the Post-IPO Bylaws), which will become effective upon the filing of our Post-IPO Certificate of Incorporation;                                                                                                       |

| • |     | the exchange of an aggregate of 4,552,650 shares of Class A common stock held by our                                                                                                                         
 Co-Founders, for an equivalent number of shares of our Class B common stock, to be effected immediately prior to the completion of this offering pursuant to the terms of an exchange agreement entered into 
 with us (the Class B Stock Exchange);                                                                                                                                                                        |

| • |     | no exercise, settlement or termination of the outstanding options or warrants described above; |

| • |     | no exercise by the underwriters of their option to purchase up to 576,900 additional shares of our Class A common 
 stock in this offering; and                                                                                       |

| • |     | does not include an additional 884,580 shares of our Class A common stock that have been registered with the SEC and                                                        
 may be issued if the number of shares included in this offering is increased, including a corresponding increase in the underwriters’ option to purchase additional shares. |

18

Summary financial data The following table sets forth our summary statements of operations data for the years ended December 31, 2023 and 2024 and the six months ended June 30, 2024 and 2025, as well as our summary balance sheet data as of June 30, 2025. We derived the summary statements of operations data for the years ended December 31, 2023 and 2024 from our audited financial statements included elsewhere in this prospectus. The summary condensed statements of operations data for the six months ended June 30, 2025 and 2024 and summary balance sheet data as of June 30, 2025 have been derived from our unaudited financial statements included elsewhere in this prospectus. Our historical results are not necessarily indicative of the results that may be expected in the future. When you