Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 9

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 9
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 the total number of outstanding shares of Common Stock held by non-affiliates of our Company, in each case as of October 8, 2025.

Although the Purchase Agreement provides that we may sell up to $100,000,000
of our Common Stock to Ascent, only an additional 42,211,548 Purchase Shares are being registered under the Securities Act for resale
by Ascent under the registration statement which includes this prospectus. This amount is in addition to the 8,970,830 shares previously
registered for resale in our prior registration statement, which was declared effective on August 25, 2025, including 900,000 Commitment
Warrant Shares (for which we have not and will not receive any cash consideration). If we were to issue and sell all of such 42,211,548
Purchase Shares to Ascent at an assumed purchase price per share of $0.34, representing the closing sale price of our Common Stock on
Nasdaq on October 7, 2025, we would only receive approximately $14.4 million in aggregate gross proceeds from the sale of such
Purchase Shares to Ascent under the Purchase Agreement. Depending on the market prices of our Common Stock on the Purchase Dates on which
we elect to sell such Purchase Shares to Ascent under the Purchase Agreement, we may in the future need to register under the Securities
Act additional shares of our Common Stock for resale by Ascent which, together with the 42,211,548 Purchase Shares included in this prospectus,
will enable us to issue and sell to Ascent such aggregate number of shares of Common Stock under the Purchase Agreement as will be necessary
in order for us to receive aggregate proceeds equal to Ascent’s $100,000,000 maximum aggregate purchase commitment available to
us under the Purchase Agreement.

The number of Purchase Shares ultimately offered
for resale by Ascent through this prospectus is dependent upon the number of shares of Common Stock, if any, we elect to issue and/or
sell to Ascent under the Purchase Agreement from and after the Effective Date. The issuance of our Common Stock to Ascent pursuant to
the Purchase Agreement will not affect the rights or privileges of our existing stockholders, except that the economic and voting interests
of each of our existing stockholders will be diluted. Although the number of shares of our Common Stock that our existing stockholders
own will not decrease, the shares of our Common Stock owned by our existing stockholders will represent a smaller