Company: BLLN
Filing Date: 2025-10-17
Form Type: S-1/A
Source: 0001193125-25-242632
Chunk: 417

Company: BillionToOne, Inc.
Filing Date: 2025-10-17
Form: S-1/A
Chunk 417
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 final adjudication of such issue. The undersigned registrant hereby undertakes that: (1) For purposes of determining any liability under the Securities Act, the information omitted from the form of prospectus filed as part of this Registration Statement in reliance upon Rule 430A and contained in a form of prospectus filed by the registrant pursuant to Rule 424(b)(1) or (4) or 497(h) under the Securities Act shall be deemed to be part of this Registration Statement as of the time it was declared effective. (2) For the purpose of determining any liability under the Securities Act, each post-effective amendment that contains a form of prospectus shall be deemed to be a new registration statement relating to the securities offered therein, and the offering of such securities at that time shall be deemed to be the initial bona fide offering thereof. II-5

Signatures Pursuant to the requirements of the Securities Act of 1933, as amended, the Registrant has duly caused this Registration Statement to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Menlo Park, State of California, on October 17, 2025.

| BillionToOne, Inc. |     |                         |
| By:                |     | /s/ Oguzhan Atay        |
|                    |     | Oguzhan Atay            |
|                    |     | Chief Executive Officer |

Pursuant to the requirements of the Securities Act of 1933, as amended, this Registration Statement has been signed by the following persons in the capacities and on the dates indicated.

| Signature               
 /s/ Oguzhan Atay        
 Oguzhan Atay            
 /s/ Ross Taylor         
 Ross Taylor             |     | Title                                        
 Chief Executive Officer and Director         
 (Principal Executive Officer)                
 Chief Financial Officer                      
 (Principal Financial and Accounting Officer) |     | Date             
 October 17, 2025 
 October 17, 2025 |
|:------------------------|:----|:---------------------------------------------|:----|:-----------------|
| *                       
 David Tsao              |     | Chief Technology Officer and Director        |     | October 17, 2025 |
| *                       
 Thomas Bremner          |     | Director                                     |     | October 17, 2025 |
| *                       
 Firat Ileri             |     | Director                                     |     | October 17, 2025 |
| *                       
 Krishna Swaroop Kolluri |     | Director