Company: BLLN
Filing Date: 2025-06-20
Form Type: DRS
Source: 0000950123-25-006095
Chunk: 4

Company: BillionToOne, Inc.
Filing Date: 2025-06-20
Form: DRS
Chunk 4
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the United States are required to inform themselves about and to observe any restrictions relating to this offering and the distribution of this prospectus.

Glossary

As used in this prospectus, unless we state otherwise or the context otherwise requires:

ACOG: American College of Obstetricians and Gynecologists

ASP: Average
selling price—The average payment BillionToOne receives per test (inclusive of any non-payments)

CF: Cystic
fibrosis—A genetic disorder that can be detected by UNITY tests

cfDNA: Cell-free DNA—Extracellular DNA in blood plasma

CNV: Copy number variant—A variation in gene copies that Northstar Select can detect

ctDNA: Circulating tumor DNA—Cell-free DNA released from tumor cells

MolDX: Molecular Diagnostic Services program – establishes coverage and reimbursement for Medicare beneficiaries

MRD: Minimal residual disease—cfDNA remaining after surgery or treatment

NGS:Single-molecule next-generation sequencing

NIPT: Non-invasive prenatal testing—Testing that uses cell-free DNA from maternal blood to assess fetal genetic risks

PCR:Polymerase Chain Reaction- a laboratory technique that amplifies, or creates multiple copies of, a specific DNA sequence

QCT: Quantitative Counting
Templates—BillionToOne’s patented synthetic DNA fragments that are the foundation of the smNGS platform

SCD: Sickle cell disease—A hereditary
red blood cell disorder that can be detected by UNITY tests

sgNIPT: Single-gene non-invasive prenatal testing—A
type of test pioneered by BillionToOne that can detect single-gene disorders without paternal sample

SMA: Spinal muscular atrophy—A genetic disorder
that can be detected by UNITY tests

smNGS: Single-molecule next-generation sequencing—BillionToOne’s platform technology that enables detection and
quantification of blood-based genetic targets with single molecule sensitivity

SNV: Single nucleotide variant—A variation in a gene’s sequence that
Northstar Select can detect

TMS: Tumor Methylation Score – Northstar Response’s metric that measures the quantity of cancer-specific DNA
methylation in blood for monitoring response to a cancer therapy

Prospectus summary

This summary highlights information contained elsewhere in this prospectus and does not contain all of the information that you should consider in making your investment decision. Before investing in our Class A common stock