Company: TEM
Filing Date: 2025-02-25
Form Type: S-1
Source: 0001193125-25-034442
Chunk: 4

Company: Tempus AI, Inc.
Filing Date: 2025-02-25
Form: S-1
Chunk 4
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us supplement or free writing prospectus to add information to, or update or change information
contained or incorporated by reference in, this prospectus. You should read this prospectus, any applicable prospectus supplement or free writing prospectus, including the documents we incorporate herein and therein by reference, together with the
additional information to which we refer you in the section of this prospectus entitled “Where You Can Find More Information.”

This prospectus contains summaries of certain provisions contained in some of the documents described herein, but reference is
made to the actual documents for complete information. All of the summaries are qualified in their entirety by the actual documents. Copies of some of the documents referred to herein have been filed, will be filed or will be incorporated by
reference as exhibits to the registration statement of which this prospectus is a part, and you may obtain copies of those documents as described below under “Where You Can Find More Information.”

ii

SPECIAL NOTE REGARDING FORWARD-LOOKING STATEMENTS This prospectus and any applicable prospectus supplement or free writing prospectus, including the documents that we incorporate by reference herein and therein, contain forward-looking statements about us and our industry that involve substantial risks and uncertainties. All statements other than statements of historical facts, including statements regarding our future results of operations or financial condition, business strategy and plans and objectives of management for future operations, are forward-looking statements. In some cases, you can identify forward-looking statements because they contain words such as “anticipate,” “believe,” “contemplate,” “continue,” “could,” “estimate,” “expect,” “intend,” “may,” “plan,” “potential,” “predict,” “project,” “should,” “target,” “will” or “would” or the negative of these words or other similar terms or expressions. These forward-looking statements include, but are not limited to, statements concerning the following:

| • |     | the evolving treatment paradigm for cancer, including physicians’ use of molecular data and targeted 
 oncology therapeutics and the market size for our current and future products;                       |

| • |     | our ability to expand our business beyond oncology into new disease areas; |

| • |     | estimates of our addressable market and our expectations regarding our revenue, expenses, capital requirements 
 and operating results;                                                                                         |

| • |     | our ability to develop new products and services, including our goals and strategy regarding development and 
 commercialization of AI Applications;                                                                        |

| • |     | our ability to