Company: BTBT
Filing Date: 2025-10-02
Form Type: 305B2
Source: 0001213900-25-095536
Chunk: 0

Company: Bit Digital, Inc
Filing Date: 2025-10-02
Form: 305B2
Chunk 0
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<div align='center'>securities and exchange commission

Washington, D.C. 20549

FORM T-1

Statement of Eligibility Under

The Trust Indenture Act of 1939 of a

Corporation Designated to Act as Trustee

Check if an Application to Determine Eligibility
of

a Trustee Pursuant to Section 305(b)(2)☒

U.S. BANK TRUST COMPANY, NATIONAL ASSOCIATION

(Exact name of Trustee as specified in its charter)

91-1821036

I.R.S. Employer Identification No.</div>

| 800 Nicollet Mall                        
 Minneapolis, Minnesota                   |     |     55402 |
| (Address of principal executive offices) |     | -Zip Code |

<div align='center'>Quinton M. DePompolo

U.S. Bank Trust Company, National Association

60 Livingston Avenue

St. Paul, MN 55107

(651) 466-7108

(Name, address and telephone number of agent for
service)

Bit Digital, Inc.

(Issuer with respect to the Securities)</div>

| Cayman Islands                                                 |     | 98-1606989                          |
| (State or other jurisdiction of incorporation or organization) |     | -I.R.S. Employer Identification No. |

| 31 Hudson Yards, Floor 11                
 New York, NY                             |     |     10001 |
| (Address of Principal Executive Offices) |     | -Zip Code |

<div align='center'>Debt Securities

(Title of the Indenture Securities)

FORM T-1</div>

| Item 1. | GENERAL INFORMATION. Furnish the following information as to the Trustee. |

| a) | Name and address of each examining or supervising authority to which it is subject. |

Comptroller of the Currency

Washington, D.C.

| b) | Whether it is authorized to exercise corporate trust powers. |

Yes

| Item 2. | AFFILIATIONS WITH THE OBLIGOR.  If the obligor is an affiliate of the Trustee, 
 describe each such affiliation.                                                |

None

| Items 3-15 | Items 3-15 are not applicable because to the best of the Trustee’s knowledge,            
 the obligor is not in default under any Indenture for which the Trustee acts as Trustee. |

| Item 16. | LIST OF EXHIBITS: List below all