Company: GAINI
Filing Date: 2025-06-20
Form Type: DEF 14A
Source: 0001193125-25-143255
Chunk: 0

Company: GLADSTONE INVESTMENT CORPORATION\DE
Filing Date: 2025-06-20
Form: DEF 14A
Chunk 0
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UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a party other than the Registrant ☐ Check the appropriate box:

| ☐ | Preliminary Proxy Statement |

| Confidential, for Use of the Commission Only (as permitted by Rule14a-6(e)(2)) |

| ☒ | Definitive Proxy Statement |

| ☐ | Definitive Additional Materials |

| ☐ | Soliciting Material Under§240.14a-12 |

GLADSTONE INVESTMENT CORPORATION (Name of Registrant as Specified In Its Charter) Not Applicable (Name of Person(s) Filing Proxy Statement, If Other Than The Registrant) Payment of Filing Fee (Check the appropriate box):

| ☒ | No fee required. |

| ☐ | Fee paid previously with preliminary materials. |

| ☐ | Fee computed on table below per Exchange Act Rules14a-6(i)(1)and0-11 |

GLADSTONE INVESTMENT CORPORATION 1521 Westbranch Drive, Suite 100, McLean, Virginia 22102 NOTICE OF ANNUAL MEETING OF STOCKHOLDERS To Be Held On August 7, 2025 To the Stockholders of Gladstone Investment Corporation: Notice is hereby given that the 2025 Annual Meeting of Stockholders of Gladstone Investment Corporation (the “Annual Meeting”) will be held on Thursday, August 7, 2025, at 11:00 a.m., Eastern Time. The Annual Meeting will be a completely “virtual meeting.” You will be able to attend the meeting, as well as vote and submit your questions during the live webcast of the meeting, by visiting www.virtualshareholdermeeting.com/GAIN2025and entering the company number and control number included on your proxy card or in the instructions that accompany your proxy materials. At the Annual Meeting, you will be asked to consider and vote upon the following proposals:

| 1) | To elect two directors to hold office for terms that will expire at the 2028 Annual Meeting of Stockholders and until their respective successors are duly elected and qualified. |

The foregoing items of business are more fully described in the Proxy Statement accompanying this Notice. Our Board of Directors has fixed the close of business on June 11, 2025 as the record date for the determination of