Company: GAINI
Filing Date: 2025-06-20
Form Type: DEF 14A
Source: 0001193125-25-143255
Chunk: 39

Company: GLADSTONE INVESTMENT CORPORATION\DE
Filing Date: 2025-06-20
Form: DEF 14A
Chunk 39
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or scan the QR Barcode above Use the Internet to transmit your voting instructions and for electronic delivery of information up until 11:59 p.m. Eastern Time the day before the cut-offdate or meeting date. Have your proxy card in hand when you access the web site and follow the instructions to obtain your records and to create an electronic voting instruction form. During The Meeting - Go to www.virtualshareholdermeeting.com/GAIN2025 You may attend the Meeting via the Internet and vote during the Meeting. Have the information that is printed in the box marked by the arrow available and follow the instructions. VOTE BY PHONE - 1-800-690-6903 Use any touch-tone telephone to transmit your voting instructions up until 11:59 p.m. Eastern Time the day before the cut-offdate or meeting date. Have your proxy card in hand when you call and then follow the instructions. VOTE BY MAIL Mark, sign and date your proxy card and return it in the postage-paid envelope we have provided or return it to Vote Processing, c/o Broadridge, 51 Mercedes Way, Edgewood, NY 11717. TO VOTE, MARK BLOCKS BELOW IN BLUE OR BLACK INK AS FOLLOWS:

| V74430-P33941 |     | KEEP THIS PORTION FOR YOUR RECORDS |

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| DETACH AND RETURN THIS PORTION ONLY |

THIS PROXY CARD IS VALID ONLY WHEN SIGNED AND DATED.

| GLADSTONE INVESTMENT CORPORATION                                                         |     | For 
 All |     | Withhold All |     | For All Except |
| The Board of Directors recommends you vote FOR the following:                            |     | ☐   |     | ☐            |     | ☐              |
| 1.   Election of Directors to hold office until the 2028 Annual Meeting of Stockholders. |     |     |     |              |     |                |
| Nominees:   01)  David Gladstone 02)  John H. Outland                                    |     |     |     |              |     |                |

| To withhold authority to vote for any individual nominee(s), mark "For All Except" and write the number(s) of