Company: REVB
Filing Date: 2025-04-04
Form Type: DRS
Source: 0001213900-25-029022
Chunk: 100

Company: REVELATION BIOSCIENCES, INC.
Filing Date: 2025-04-04
Form: DRS
Chunk 100
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. infection) by producing and releasing various types of cytokines. Cytokines are proteins that direct different activities in cells and can be inflammatory or protective, meaning they may be able to modulate certain established cellular activities. Toll -likereceptors serve a vital role in initiating the innate immune system response by recognizing different molecular patterns associated with pathogens such as bacteria and viruses (e.g. PAMPS: pathogen associated molecular patterns) as well as tissue damage (e.g. DAMPS: damage associated molecular patterns). Revelation believes immunostimulatory preconditioning with Gemini prepares the body to respond better to stress due to infection, trauma, or other acute and chronic disorders. Modulated activities may include stimulation and recruitment of infection fighting immune cells, reduction of inflammation, and/or regulation of inflammation depending on the degree and nature of the stimulation which enables the multiple potential product indications in development. Figure 1: Interaction of PHAD with TLR4 Source: Revelation Biosciences 62 Gemini-AKI Program Overview Gemini is being evaluated as a potential therapy for the prevention of AKI due to external stress (e.g. surgical procedure, chemotherapy toxicity). We believe immunologic preconditioning with Gemini prepares the body to handle biologic stress by directing the body’s immune system to have an attenuated response to the stress. Gemini is being evaluated as a potential therapy for the prevention of AKI. Preclinical Preclinical studies have shown that pretreatment with PHAD results in significantly decreased severity and duration of acute kidney injury due to ischemia. PHAD Pretreatment Reduces AKI in a Unilateral Ischemia / Reperfusion Model (1) ____________ (1)Mice pretreated with intravenous PHAD at 2, 20, and 200 µg/mouse or vehicle control, 48 and 24 hours prior to undergoing right nephrectomy followed by clamping of the left renal pedicle for 28 minutes. A) Blood was analyzed for BUN and creatinine at baseline (D0), and post -injuryday 1 and 3. Results expressed as means +/ -SEMwith N = 8. Two -wayANOVA was used to compare differences between PHAD- and vehicle -treatedmice over time, with p values indicated; B) Representative images of periodic acid -Schiffstaining (PAS) sections of the outer medulla at Day 3 after injury in sham, vehicle- and PHAD -treatedmice. Arrows point to casts within