Company: MDCXW
Filing Date: 2025-03-07
Form Type: 253G1
Source: 0001062993-25-004966
Chunk: 106

Company: Medicus Pharma Ltd.
Filing Date: 2025-03-07
Form: 253G1
Chunk 106
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 receiving the 200 µg D-MNA; doxorubicin levels were detectable in the intravenously injected mice.

<div align='center'>Figure 10

- 73 -</div>

In addition, Medicus obtained pharmacokinetic data during the Yucatan minipig local tolerance study (Study No. S15055). A total of 30 minipigs (15 males and 15 females) were randomized into five treatment groups. Groups of 3 males and 3 females were treated with a blank control array (Group 1), and three dose levels of D-MNA at 25, 50 and 200 µg/dose of doxorubicin hydrochloride (Groups 2 - 4), respectively. An intravenous ("IV") doxorubicin group consisting of 3 males and 3 females served as a reference control (Group 5). Dosing occurred on Days 1, 7 and 14 with either control array or test article (Groups 1-4), and Group 5 was dosed IV on these same study days. Each topical treatment was 30 minutes. The experimental design is provided in the table below.

<div align='center'>- 74 -</div>

Study Design of Minipig Local Tolerance Study

| Group | Treatment         | Treatment/Dose |                                      | Number of Animals |   | Dose Route/ Frequency |
| Array | Dosage (µg/dose)  |           Male |                               Female |                   |   |                       |
| 1     | Blank Control     |              1 |                                    0 |                 3 | 3 | MNA/Days 1, 7, 14     |
| 2     | D-MNA-low         |              1 |                                   25 |                 3 | 3 |                       |
| 3     | D-MNA-mid         |              1 |                                   50 |                 3 | 3 |                       |
| 4     | D-MNA-high        |              1 |                                  200 |                 3 | 3 |                       |
| 5     | Reference Control |             NA | 2 mg/kg (Doxorubicin Hydrocholoride) |                 3 | 3 | IV/Days, 1, 7,        
 14                    |

Note:

(1)Dosing volume for Group 5 was 1 ml/kg (doxorubicin hydrocholor