Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 133

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 133
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, relief of debtors, dissolution, insolvency, winding up, reorganization, arrangement,   
 adjustment, protection, relief or composition of debts or liquidation or similar regulation of any jurisdiction relating to the Company 
 or such subsidiary;                                                                                                                     |

| ● | no change of control of the Company shall have occurred; |

| ● | the Company shall not have amended its charter documents in any manner that materially and adversely affects 
 any rights of the Ascent or change the nature of the Company’s business;                                     |

| ● | the Company’s Common Stock shall not have become “penny stock” as defined in Regulations 
 for purposes of 3(a)(51) of the Exchange Act, and                                        |

| ● | the transfer of shares of Common Stock through the Depository Trust Company System shall remain available                              
 for the “Deposit and Withdrawal at Custodian” (DWAC) service of the Deposit Trust Corporation and shall not be subject to              
 any restriction or limitation imposed by or on behalf of the Deposit Trust Corporation on any of its services or any other restriction 
 or limitation on the use of the services provided by the Deposit Trust Corporation (DTC chill).                                        |

<div align='center'>72</div>

Termination of the Purchase Agreement

The Purchase Agreement will
automatically terminate on the earliest to occur of (i) July 27, 2028, (ii) the date on which Ascent shall have purchased from us under
the Purchase Agreement shares of Common Stock for an aggregate gross purchase price of $100,000,000, and (iii) the date either party effectively
delivers notice to the other party of such termination.

No Short-Selling or Hedging by Ascent

Ascent has represented to us
that at no time prior to the date of the Purchase Agreement has Ascent, any of its officers, or any entity managed or controlled by Ascent,
engaged in or effected, in any manner whatsoever, directly or indirectly, for Ascent’s own principal account or for the principal
account of any such entity managed or controlled by Ascent, any short sale (as such term is defined in Rule 200 of Regulation SHO of the
Exchange Act) of our Common Stock or any hedging transaction, which establishes a net short position with respect to our Common Stock
that remained in effect as of the date of the Purchase Agreement. Ascent has agreed that, during the term of the Purchase Agreement, none
of Ascent, any of its officers, or any entity managed