Company: BLLN
Filing Date: 2025-10-07
Form Type: S-1
Source: 0001193125-25-233697
Chunk: 398

Company: BillionToOne, Inc.
Filing Date: 2025-10-07
Form: S-1
Chunk 398
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 2025 |     |             | 8,933,419 |   |     | $              | 10.59 |     |             | 7.8 |     | $               | 112,216 |

The intrinsic value is calculated as the difference between the exercise price of the underlying stock option award and the estimated fair value of the Company’s common stock. The total intrinsic values of options exercised during the six months ended June 30, 2024 and 2025 $2.7 million and $2.8 million, respectively. During the six months ended June 30, 2024, the weighted average grant-date fair value of options granted was $10.27 per share, and the fair value of options vested during the period was $3.2 million. During the six months ended June 30, 2025, the weighted average grant-date fair value of options granted was $15.65 per share, and the fair value of options vested during the period was $4.7 million. The fair value is being expensed over the vesting period of the options, on a straight-line basis as the services are being provided. As of June 30, 2025, there was approximately $53.2 million of total unrecognized compensation cost related to unvested options, which is expected to be recognized over the weighted average period of 3.9 years. Also, in June 2025, the Board of Directors granted stock options of 1,003,102 shares to the Company’s CEO and Co-Founderand 501,551 shares to the Chief Technology Officer and Co-Founderat an exercise price of $20.04 per share. The vesting commencement date of the stock option is June 11, 2025 and the stock options vests monthly over six years. F-59

BILLIONTOONE, INC. Notes to Unaudited Interim Financial Statements Stock-Based Compensation Expense The table below shows stock-based compensation expense included in the statements of operations and comprehensive loss for the six months ended June 30, 2024 and 2025 (in thousands):

|                                     |     |   | June 30, 
     2024 |     |   | June 30, 
     2025 |
|:------------------------------------|:----|:--|---------:|:----|:--|---------:|
| Cost of revenue                     |     | $ |      564 |     | $ |      738 |
| Research and development