Company: BLLN
Filing Date: 2025-09-17
Form Type: DRS/A
Source: 0001193125-25-206347
Chunk: 165

Company: BillionToOne, Inc.
Filing Date: 2025-09-17
Form: DRS/A
Chunk 165
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 expense.

|                               |     | 2023 | Years ended December 31, |   |     | 2024 |         |   |     | 2024 | Six months ended June 30, |   |     | 2025 |        |   |
|:------------------------------|:----|:-----|-------------------------:|:--|:----|:-----|--------:|:--|:----|:-----|--------------------------:|:--|:----|:-----|-------:|:--|
| Net loss                      |     | $    |                  (82,683 | ) |     | $    | (41,570 | ) |     | $    |                   (15,189 | ) |     | $    | (4,234 | ) |
| Income taxes                  |     |      |                        9 |   |     |      |      29 |   |     |      |                         5 |   |     |      |    114 |   |
| Interest (income)             |     |      |                   (3,456 | ) |     |      |  (5,819 | ) |     |      |                    (2,196 | ) |     |      | (2,957 | ) |
| Interest expense              |     |      |                    3,272 |   |     |      |   2,386 |   |     |      |                     1,917 |   |     |      |     72 |   |
| Depreciation and amortization |     |      |                    5,874 |   |     |      |   7,040 |   |     |      |                     3,432 |   |     |      |  3,540 |   |
| EBITDA                        |     | $    |                  (76,984 | ) |     | $    | (37,934 | ) |     | $    |                   (12,031 | ) |     | $    | (3,465 | ) |

Adjusted EBITDA We define Adjusted EBITDA as net loss adjusted for income taxes, interest income, interest expense, depreciation and amortization expense, and certain other items which include significant non-cashitems events 111

that are highly variable, significant in size, and that we do not believe are indicative of ongoing or future business operations, which include: stock-based compensation expense; net gain on extinguishment of debt