Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 243

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 243
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 balance of the shares issuable under the Initial Note and the Second Tranche Note are not included in this column because these 
 share issuances are subject to a beneficial ownership limitation of 9.99%.                                                              |

| (6) | Comprised of 441,395 conversion shares held by Ascent as of October 8, 2025 and 8,878,333 shares issuable upon conversion of the Initial Note and the Second Tranche Note. The balance of the shares issuable under the Initial Note and the Second Tranche Note are not included in this column because these share issuances are subject to a beneficial ownership limitation of 9.99%. |

<div align='center'>144

PLAN OF DISTRIBUTION</div>

We are registering the shares
of common stock issuable to the Selling Stockholders to permit the resale of these shares of Common Stock by the holders of the shares
of Common Stock from time to time after the date of this prospectus. We will not receive any of the proceeds from the sale by the Selling
Stockholders of the shares of Common Stock. We will bear all fees and expenses incident to the registration of the shares of Common Stock.

The Selling Stockholders
may sell all or a portion of the shares of Common Stock beneficially owned by them and offered hereby from time to time directly or through
one or more underwriters, broker-dealers or agents. If the shares of Common Stock are sold through underwriters or broker-dealers, the
Selling Stockholders will be responsible for underwriting discounts or commissions or agent’s commissions. The shares of Common
Stock may be sold on any national securities exchange or quotation service on which the securities may be listed or quoted at the time
of sale, in the over-the-counter market or in transactions otherwise than on these exchanges or systems or in the over-the-counter market
and in one or more transactions at fixed prices, at prevailing market prices at the time of the sale, at varying prices determined at
the time of sale, or at negotiated prices. These sales may be effected in transactions, which may involve crosses or block transactions.

The Selling Stockholders may use any one or more
of the following methods when selling shares:

| ● | ordinary brokerage transactions and transactions in which the broker-dealer solicits purchasers;                                                                                |
| ● | block trades in which the broker-dealer will attempt to sell the shares as agent but may position and resell a portion of the block as principal to facilitate the transaction; |
| ● | purchases