Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 155

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 155
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. | Reflects the impact from the warrants issued to Ascent according to the ELOC Registration Rights Agreement. 
 The warrants are equity classified and recorded at their fair value on the agreement date.                  |

| T. | Reflects the cash received and the shares issued from the ELOC facility,                                                                
 assuming additional 42,211,548 shares issued at the floor price of $0.14 per share for approximately $5.9 million additional financing, 
 plus the 8,970,830 previously registered shares assuming an average conversion price using the VWAP rate on August 26, 2025 of $0.4743  
 per share.                                                                                                                              |

<div align='center'>87</div>

Transaction Accounting Adjustments to Unaudited Pro Forma Condensed Combined Statements of Operations

The Transaction Accounting Adjustments
included in the unaudited pro forma condensed combined statements of operations for the six months ended June 30, 2025 and the year
ended December 31, 2024 are as follows:

| AA. | Represents the elimination of investment income related to the investments held in the NorthView Trust 
 Account.                                                                                               |

| CC. | Represents the income tax impact on the elimination of investment income related to the investments held 
 in NorthView’s Trust Account.                                                                            |

| DD. | Represents elimination of the changes in the fair value of Northview’s convertible loan upon the 
 closing of the Business Combination.                                                             |

| GG. | Represents issuance of the Milestone Earnout Rights and Profusa Inducement Recoupment Earnout Rights to                                 
 the equity holders of Profusa, which are treated as dividend distributions and recorded in additional paid-in capital. Earnout right    
 represents a right to receive shares in future upon meeting certain earnout targets. The issuance date fair values of Milestone Earnout 
 Rights and Profusa Inducement Recoupment Earnout Rights was determined using Monte Carlo Simulation approach. The Company estimated the 
 vesting and payoff of the Milestone Earnout Shares and the Inducement Shares related to Milestone I Event and Milestone II Event        
 for each simulated stock price path, and the vesting and payoff of the Milestone Earnout Shares related to Milestone III Event and      
 Milestone IV Event for each simulated revenue path and the correlated stock price path. The fair value is then determined by averaging  
 the payoff across all simulated paths and discounting it to the valuation date.                                                         |

| HH. | Reflects transaction costs incurred in excess of