Company: GAINI
Filing Date: 2025-08-12
Form Type: 40-APP/A
Source: 0001193125-25-178985
Chunk: 0

Company: GLADSTONE INVESTMENT CORPORATION\DE
Filing Date: 2025-08-12
Form: 40-APP/A
Chunk 0
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File No. 812-15806

U.S. SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

In the Matter of the Application of:

GLADSTONE ALTERNATIVE INCOME FUND; GLADSTONE CAPITAL CORPORATION;

GLADSTONE INVESTMENT CORPORATION; GLADSTONE BUSINESS LOAN, LLC; GLADSTONE

BUSINESS INVESTMENT, LLC AND GLADSTONE MANAGEMENT CORPORATION

1521 Westbranch Drive, Suite 100

McLean, Virginia 22102

Telephone: (703) 287-5800

AMENDMENT NO. 1 TO APPLICATION FOR AN ORDER PURSUANT TO SECTIONS 17(d) AND 57(i) OF THE INVESTMENT

COMPANY ACT OF 1940 AND RULE 17d-1UNDER THE INVESTMENT COMPANY ACT OF 1940

PERMITTING CERTAIN JOINT TRANSACTIONS OTHERWISE PROHIBITED BY SECTIONS 17(d)

AND 57(a)(4) OF AND RULE 17d-1 UNDERTHE INVESTMENT COMPANY ACT OF 1940

All Communications, Notices and Orders to:

David Gladstone

Chief Executive Officer

Gladstone Management Corporation

1521 Westbranch Drive, Suite 100

McLean, Virginia 22102

Telephone: (703) 287-5800

david.gladstone@gladstone.com

Copies to:

William J. Tuttle, P.C.

Erin M. Lett

Kirkland & Ellis LLP

1301 Pennsylvania Avenue, NW

Washington, DC 20004

(202) 389-5000

william.tuttle@kirkland.com

erin.lett@kirkland.com

August 12, 2025

UNITED STATES OF AMERICA BEFORE THE SECURITIES AND EXCHANGE COMMISSION

|                                  |     | : |     | AMENDMENT NO. 1 TO APPLICATION FOR AN ORDER |
| IN THE MATTER OF                 |     | : |     | PURSUANT TO SECTIONS 17(d) AND 57(i)        |
|                                  |     | : |     | OF THE INVESTMENT COMPANY ACT OF            |
| GLADSTONE ALTERNATIVE INCOME     |     | : |     | 1940 AND RULE 17d-1 UNDER THE               |
| FUND; GLADSTONE CAPITAL          |     | : |     | INVESTMENT COMPANY ACT OF 194