Company: NIVFW
Filing Date: 2025-09-18
Form Type: F-1
Source: 0001213900-25-088927
Chunk: 25

Company: NewGenIvf Group Ltd
Filing Date: 2025-09-18
Form: F-1
Chunk 25
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 eggs in the follicles before they are collected. The next major step is to retrieve the eggs with a minor surgical procedure called
Trans Vaginal Follicle Aspiration conducted under anaesthesia. At the same time the male partner collects the sperms for fertilizing the
eggs in the laboratory by a process known as intracytoplasmic sperm injection. The fertilized embryos are cultured in the laboratory for
two to six days. Embryos that grow well are biopsied and tested by PGT to detect potential genetic diseases.

<div align='center'>9</div>

The final step is to transfer
the embryos into the uterus using a catheter. Within eight days after the embryo transfer, a blood test can be conducted to detect
whether the implantation was successful.

MicroSort Technology

MicroSort technology is a
preconception process developed by the Genetics & IVF Institute, Inc (“GIVF”). that aims to improve the chances that the
baby to be conceived will be of the desired gender and prevents certain gender-related hereditary diseases.

Semen samples usually contain
equal amounts of sperm carrying the Y chromosome (which will produce a boy), and sperm carrying the X chromosome (which will produce
a girl). During the MicroSort process, the sperm sample is washed to remove seminal liquid and nonmotile cells. After the washing, the
sample is stained with a special fluorescent material that attaches to the DNA contained in the sperm. The stained sperm cells are analyzed
one by one by a flow cytometer, in which cells pass through a laser to make the stain attach to the DNA fluoresce. The sperm containing
the X chromosome (which have more DNA and therefore more stain) will shine brighter than the sperm containing the Y chromosome. The
flow cytometer uses a special software to identify X and Y chromosome sperm based on their fluorescence signature. The sperm
carrying the chromosome that will produce the desired gender are separated from the rest of the sample -resulting in an enriched sperm
sample ready for use.

NewGenIvf held an exclusive
license granted by a division of GIVF, MicroSort International, to use the MicroSort technology in Thailand and Cambodia. MicroSort’s
licenses for NewGenIvf’s operation in Thailand and Cambodia were each provided under a lease and service agreement. In April 2019,
First Fertility PGS entered into a Lease and Services Agreement with MicroSort International to use MicroSort equipment in Thailand and
in March 2019,