Company: ARTL
Filing Date: 2025-09-30
Form Type: 424B5
Source: 0001640334-25-001749
Chunk: 2

Company: ARTELO BIOSCIENCES, INC.
Filing Date: 2025-09-30
Form: 424B5
Chunk 2
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 for certain expenses incurred in this offering. See “Underwriting” on page S-15 of this prospectus supplement for more information regarding the underwriting compensation. |
| (2) | Assumes the underwriter does not exercise its over-allotment option.                                                                                                                                         |

We have granted the underwriter an option for a period ending 45 days after the closing date of this offering to purchase up to an additional [ ] shares of common stock at the public offering price, less the underwriting discounts, solely to cover over-allotments, if any. If the underwriter exercises the option in full, the total underwriting discounts will be $[ ], and the total proceeds to us, before expenses, will be $[ ].

The underwriter expects to deliver the shares of common stock and the Pre-funded Warrants on or about , 2025.

<div align='center'>Sole Book Running Manager

R.F. Lafferty & Co., Inc.

The date of this prospectus supplement is [ ], 2025.

TABLE OF CONTENTS

Prospectus Supplement</div>

|                                           |     | Page  |
| About This Prospectus Supplement          |     | S-iii |
| Forward-Looking Statements                |     | S-iii |
| Prospectus Supplement Summary             |     | S-1   |
| The Offering                              |     | S-5   |
| Risk Factors                              |     | S-6   |
| Use of Proceeds                           |     | S-9   |
| Capitalization                            |     | S-10  |
| Dilution                                  |     | S-11  |
| Description of Securities We Are Offering |     | S-12  |
| Underwriting                              |     | S-15  |
| Legal Matters                             |     | S-18  |
| Experts                                   |     | S-18  |
| Where You Can Find Additional Information |     | S-18  |
| Incorporation of Documents by Reference   |     | S-18  |

<div align='center'>Prospectus</div>

|                                     |     | Page |
| About this Prospectus               |     |    3 |
| Prospectus Summary                  |     |    4 |
| Risk Factors                        |     |    9 |
| Forward‑Looking Statements          |     |    9 |
| Use of Proceeds                     |     |    9 |
| Description of Capital Stock        |     |   10 |
| Description of Debt Securities