Company: BIAF
Filing Date: 2025-05-27
Form Type: 424B5
Source: 0001641172-25-012410
Chunk: 1

Company: bioAffinity Technologies, Inc.
Filing Date: 2025-05-27
Form: 424B5
Chunk 1
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 to WallachBeth with respect to certain liabilities, including liabilities under the Securities Act.

As of the date of this prospectus supplement, the aggregate market value of our outstanding shares of common stock held by non-affiliates, or the public float, was $26,742,880, which was calculated based on 24,534,752 outstanding shares of common stock held by non-affiliates at a price of $1.09 per share of common stock, the closing price of our common stock on April 1, 2025, as reported on the Nasdaq Capital Market. Pursuant to General Instruction I.B.6 of Form S-3, in no event will we sell shares of our common stock pursuant to this prospectus supplement with a value of more than one-third of the aggregate market value of our common stock held by non-affiliates in any 12-month period, so long as the aggregate market value of our common stock held by non-affiliates is less than $75,000,000. During the prior twelve calendar month period that ends on and includes the date hereof, we have sold $3,112,782 shares of our common stock pursuant to General Instruction I.B.6 to Form S-3.

Our common stock is listed on the Nasdaq Capital Market under the symbol “BIAF.” On May 22, 2025, the last reported sale price of our common stock on the Nasdaq Capital Market was $0.2753 per share.

Investing in our common stock involves a high degree of risk. Before making an investment decision, please read the information under the heading “Risk Factors” beginning on page S-4 of this prospectus supplement and in the documents incorporated by reference into this prospectus supplement and the accompanying base prospectus.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or passed upon the adequacy or accuracy of this prospectus supplement or the accompanying base prospectus. Any representation to the contrary is a criminal offense.

<div align='center'>WallachBeth Capital LLC

The date of this prospectus supplement is May 27, 2025</div>

<div align='center'>TABLE OF CONTENTS</div>

PROSPECTUS SUPPLEMENT

|                                                   | Page |
| ABOUT THIS PROSPECTUS SUPPLEMENT                  | S-ii |
| SPECIAL NOTE ON FORWARD-LOOKING STATEMENTS        | S-iv |
| PROSPECTUS SUPPLEMENT SUMMARY                     | S-1