Company: PFSA
Filing Date: 2025-08-25
Form Type: 424B3
Source: 0001213900-25-080387
Chunk: 301

Company: Profusa, Inc.
Filing Date: 2025-08-25
Form: 424B3
Chunk 301
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 | 0.001 |     |             |
| Options cancelled/forfeited                  |     |                   — |     |         — |   |     | $              |     — |     |             |
| Balances at June 30, 2024                    |     |           1,560,091 |     | 2,972,055 |   |     | $              |  0.40 |     |        3.53 |
| Exercisable at June 30, 2024                 |     |                     |     | 1,464,429 |   |     | $              |  0.49 |     |        4.88 |
| Vested and expected to vest at June 30, 2024 |     |                     |     | 2,972,055 |   |     |                |  0.40 |     |        3.53 |

During the three and six months ended June 30, 2025, stock option activity comprised of grants totaling 1,560,091 options, with no options exercised during the period. Intrinsic values are calculated as the difference between the exercise price of the underlying options and the fair value of the common stock for the options that had exercise prices that were lower than the fair value per share of the common stock on the date of exercise.

F-48

PROFUSA, INC. AND SUBSIDIARY
NOTES TO THE CONDENSED CONSOLIDATED FINANCIAL STATEMENTS
(UNAUDITED) Note 9 — Stock Option Plan (cont.) The total fair value of options vested for the three and six months ended June 30, 2025 and 2024 was less than $0.1 million. As of June 30, 2025, the total unrecognized stock -basedcompensation expense for stock options was $3.0 million which is expected to be recognized over a weighted -averageperiod of 1.3 years. The Company estimates the fair value of stock options using the Black Scholes option -pricingmodel. The fair value of stock options is being recognized on a straight -linebasis over the requisite service period of the awards. As of June 30, 2024, the total unrecognized stock -basedcompensation expense for stock options was less than $0.1 million which is expected to be recognized over a weighted -averageperiod of 1.1 years. The Company estimates the fair value of stock options using