Company: BLLN
Filing Date: 2025-08-11
Form Type: DRS/A
Source: 0000950123-25-007483
Chunk: 25

Company: BillionToOne, Inc.
Filing Date: 2025-08-11
Form: DRS/A
Chunk 25
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 future growth prospects. Channels for disclosure of information Following the closing of this offering, in addition to filings with the Securities and Exchange Commission (SEC), we intend to announce material information to the public via the investor relations page on our website, press releases, public conference calls, public webcasts, our X handle (@BillionToOneInc) and our LinkedIn feed. Any updates to the list of disclosure channels through which we will announce information will be posted on the investor relations page on our website. Corporate and other information We commenced operations in 2016 as a Delaware corporation. Our principal executive offices are located at 1035 O’Brien Drive, Menlo Park, CA 94025. Our telephone number is (650) 460-2551.Our website address is www.billiontoone.com. The information contained on, or that can be accessed through, our website is not incorporated by reference into this prospectus, and you should not consider any information contained on, or that can be accessed through, our website as part of this prospectus. “BillionToOne” and the BillionToOne logo, UNITY Complete, Northstar Select, Northstar Response, UNITY Screen (registered in the EU and UK), Quantitative Counting Templates, QCTs, UNITY Fetal Risk, UNITY Aneuploidy,

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UNITY Fetal Antigen, Tumor Methylation Score, and other trademarks or service marks of BillionToOne, Inc. appearing in this prospectus are the property of BillionToOne, Inc. This prospectus contains additional trade names, trademarks, and service marks of others, which are the property of their respective owners. Solely for convenience, trademarks and trade names referred to in this prospectus may appear without the ®or ™symbols. Implications of being an emerging growth company We qualify as an “emerging growth company” as defined in the Jumpstart Our Business Startups Act of 2012 (the JOBS Act). An emerging growth company may take advantage of specified reduced reporting and other burdens that are otherwise applicable generally to public companies. These provisions include:

| • |     | a requirement to have only two years of audited financial statements and only two years of related selected financial data 
 and management’s discussion and analysis of financial condition and results of operations disclosure in this prospectus;   |

| • |     | an exemption from the auditor attestation requirement in the assessment of our internal control over financial reporting 
 pursuant to Section 404 of the Sarbanes-Oxley Act of 2002;