Company: PFSA
Filing Date: 2025-08-21
Form Type: S-1/A
Source: 0001213900-25-079401
Chunk: 407

Company: Profusa, Inc.
Filing Date: 2025-08-21
Form: S-1/A
Chunk 407
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icial Ownership Limitation”); provided, that the PIPE Investors may increase or decrease the Beneficial Ownership Limitation upon at least 61 days’ prior notice to Profusa so long as such increase does not exceed 9.99% of the number of shares of Profusa Common Stock outstanding immediately after giving effect to the issuance of all shares issuable upon conversion of the Initial Note. On August 1, pursuant to a Notice and Waiver, the Beneficial Ownership Limitation was increased from 4.99% to 9.99% and we waived the requirement for the 60 days’ advance notice for such increase. II-2

The Company issued the Initial Note pursuant to the exemption from the registration requirements of the Securities Act available under Section 4(a)(2). All of the securities above were issued in reliance on the exemption from registration provided by Section 4(a)(2) of the Securities Act or Regulation D promulgated thereunder. 2025 Committed Equity Facility Commitment Warrants As consideration for Ascent’s commitment to purchase shares of Common Stock upon our execution of the term sheet relating to the Purchase Agreement, we issued Ascent Commitment Warrants to purchase 900,000shares of Common Stock and an exercise prices of $0.01 per share (the “Commitment Warrant Shares”), which Commitment Warrant Shares have a total aggregate value equal to 0.9% of Ascent’s $100,000,000 total aggregate purchase commitment under the Purchase Agreement (assuming a value per Commitment Warrant Share of $1.00). If we do not require Ascent to purchase any Purchase Shares on or before the 100 thday following the execution of the Purchase Agreement, the number of Commitment Warrant Shares underlying the Commitment Warrants will be reduced to 450,000. On July 28, 2025, we entered into the Purchase Agreement and a related Registration Rights Agreement with Ascent. Item 16. Exhibits and Financial Statement Schedules. (a) Exhibits: The Company has filed with this registration statement or incorporated by reference herein certain exhibits as specified below pursuant to Rule 12b -32under the Exchange Act.

| Exhibit No. 
 3.1         
 3.2         |     | Exhibit Description                                                                                                                                                                                                                          
 Amended and Restated Certificate of Incorporation (incorporated by reference to Exhibit 3.1 of the Current Report on Form 8-K filed December 23, 2021)                                                                                       
 Amendment to