Company: TEM
Filing Date: 2025-03-07
Form Type: 424B3
Source: 0001193125-25-049935
Chunk: 8

Company: Tempus AI, Inc.
Filing Date: 2025-03-07
Form: 424B3
Chunk 8
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 2,152,505 shares of which are subject to a lock-up for a period of one year following the closing date of the Acquisition. |

| Class A Common Stock outstanding | 167,168,911 shares (as of February 21, 2025). |

| Class B Common Stock outstanding | 5,043,789 shares (as of February 21, 2025). |

| Use of proceeds | We will not receive any of the proceeds from the sale of the Shares by the selling stockholder. |

| Market for Class A common stock | Our common stock is currently traded on the Nasdaq Global Select Market under the symbol “TEM.” |

| Risk factors | Before investing in our securities, you should carefully read and consider the information set forth in “Risk Factors” 
 beginning on page 5.                                                                                                   |

For additional information concerning the offering, see “Plan of Distribution” beginning on page 43.

4

RISK FACTORS

Investing in our Class A common stock involves a high degree of risk. You should carefully consider the risks and
uncertainties described under the heading “Risk Factors” in our Annual Report on Form 10-K for the year ended December 31, 2024, which is incorporated by reference into this prospectus in its entirety, as well as any amendment or updates
to our risk factors reflected in subsequent filings with the SEC, including any prospectus supplement hereto or any related free writing prospectus, before deciding to invest in our Class A common stock. If any of the events or developments
described therein were to occur, our business, prospects, operating results and financial condition could suffer materially, the trading price of our Class A common stock could decline, and you could lose all or part of your investment. The
risks and uncertainties described therein are not the only ones we face. Additional risks and uncertainties not presently known to us or that we currently believe to be immaterial may also adversely affect our business.

5

USE OF PROCEEDS

We are filing the registration statement of which this prospectus forms a part to permit the selling stockholder to resell the
Shares, as described in the section below titled “Selling Stockholder”.

The selling stockholder will receive
all of the net proceeds from sales of the Shares sold pursuant to this prospectus. We will not receive any proceeds from the resale of any Shares offered by this prospectus by the selling stockholder.

We, and not the selling stockholder, will pay the costs