Company: PFSA
Filing Date: 2025-10-29
Form Type: 424B3
Source: 0001213900-25-103174
Chunk: 156

Company: Profusa, Inc.
Filing Date: 2025-10-29
Form: 424B3
Chunk 156
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 proceeds. |

| II. | To account for the PIPE Investors interest at 10% related to the first tranche of the PIPE Convertible 
 Note.                                                                                                  |

| JJ. | To account for the warrant expense associated to the 900,000 warrants issued to Ascent associated with            
 the ELOC Registration Rights Agreement, calculated based on the warrant fair value on the warrant agreement date. |

| KK. | Represents elimination of the changes in the fair value of Northview’s Securities Purchase Agreement 
 upon the closing of the Business Combination.                                                        |

<div align='center'>88</div>

5. Net Loss per Share

Represents the net loss per
share calculated using the historical weighted average shares outstanding, and the issuance of additional shares in connection with the
Business Combination, assuming the shares were outstanding since January 1, 2024. As the Business Combination and related transactions
are being reflected as if they had occurred at the beginning of January 1, 2024, the calculation of weighted average shares outstanding
for basic and diluted net loss per share assumes that the shares issuable relating to the Business Combination have been outstanding for
the entire periods presented.

The unaudited pro forma condensed
combined financial information has been prepared with the actual redemptions of Public Shares by NorthView Public Stockholders for the
six months ended June 30, 2025 and for the year ended December 31, 2024:

|                                                                                         |     | Year Ended   
 December 31, 
 2024         |             |   |     | Six Months     
 Ended June 30, 
 2025           |             |   |
|:----------------------------------------------------------------------------------------|:----|:-------------|------------:|:--|:----|:---------------|------------:|:--|
| Pro forma net loss                                                                      |     | $            |     (15,256 | ) |     | $              |     (17,776 | ) |
| Deemed dividend                                                                         |     | $            |      (9,684 | ) |     |                |      (9,684 | ) |
| Pro forma net loss used in calculation of weighted-average pro forma net loss per share |     | $            |     (24,940 | ) |     | $              |     (27,460 | ) |
| Basic weighted average shares outstanding                                               |     |              | 110,534,620 |   |     |                | 111,250,017 |   |
| Pro forma net loss