Company: NOTV
Filing Date: 2025-08-29
Form Type: S-3
Source: 0001628280-25-041413
Chunk: 49

Company: Inotiv, Inc.
Filing Date: 2025-08-29
Form: S-3
Chunk 49
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 deemed to be underwriting commissions or discounts. We have also agreed to provide indemnification and contribution to Jefferies with respect to certain liabilities, including liabilities under the Securities Act or the Securities Exchange Act of 1934, as amended, or the Exchange Act.

Investing in our common shares involves certain risks. See “

#### Risk Factors

#### ” beginning on page

#### S-6
of this prospectus supplement and in the documents incorporated by reference into this prospectus supplement and the accompanying prospectus.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or passed upon the adequacy or accuracy of this prospectus supplement. Any representation to the contrary is a criminal offense.

| Jefferies |

#### The date of this prospectus supplement is , 2025

### TABLE OF CONTENTS

#### Prospectus Supplement
|                                                      |     | Page |
| ABOUT THIS PROSPECTUS SUPPLEMENT                     |     | S-2  |
| CAUTIONARY NOTE REGARDING FORWARD-LOOKING STATEMENTS |     | S-3  |
| PROSPECTUS SUPPLEMENT SUMMARY                        |     | S-4  |
| THE OFFERING                                         |     | S-5  |
| RISK FACTORS                                         |     | S-6  |
| USE OF PROCEEDS                                      |     | S-7  |
| DIVIDEND POLICY                                      |     | S-8  |
| DILUTION                                             |     | S-9  |
| PLAN OF DISTRIBUTION                                 |     | S-10 |
| LEGAL MATTERS                                        |     | S-12 |
| EXPERTS                                              |     | S-12 |
| WHERE YOU CAN FIND MORE INFORMATION                  |     | S-13 |

<div align='center'>S-1</div>

### ABOUT THIS PROSPECTUS SUPPLEMENT
This prospectus supplement is part of a registration statement that we have filed with the Securities and Exchange Commission (the “SEC”) using a “shelf” registration process. Under this shelf registration process, we may offer our common shares having an aggregate offering price of up to $50.0 million from time to time under this prospectus supplement at prices and on terms to be determined by market conditions at the time of offering.

We provide information to you about this offering of our common shares in two separate documents that are bound together: (1) this prospectus supplement, which describes the specific details regarding this offering; and (2) the accompanying