Company: BLLN
Filing Date: 2025-09-17
Form Type: DRS/A
Source: 0001193125-25-206347
Chunk: 270

Company: BillionToOne, Inc.
Filing Date: 2025-09-17
Form: DRS/A
Chunk 270
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 was paid             
 in 2025.                                                                                                                                                                                                          |

| (2) |     | The amounts in this column represent the aggregate grant date fair value of option awards granted to the named executive officer, computed in accordance with FASB ASC Topic No. 718. See Note 10 of the notes to our 
 audited financial statements included elsewhere in this prospectus for a discussion of the assumptions made by us in determining the grant date fair value of our equity awards.                                      |

| (3) |     | This amount relates to commissions earned under a sales commission arrangement established for Ms. Johnson. Pursuant to this arrangement, Ms. Johnson was eligible to earn commissions for each fiscal quarter 
 based upon her percentage achievement of specific sales targets established for each fiscal quarter.                                                                                                           |

| (4) |     | The amounts in this column reported for Dr. Atay and Mr. Taylor represent matching contributions under our 401(k) plan. The amount in this column reported for Ms. Johnson represents: (i) $9,000 in car 
 allowance for use of her personal vehicle and (ii) $11,602 in matching contributions under our 401(k) plan.                                                                                              |

Narrative explanation of compensation arrangements with our named executive officers Base salaries and annual incentive opportunities We pay each of our executives, including our named executive officers, a base salary to compensate them for services rendered to our company. As of December 31, 2024, our named executive officers base salaries were as follows: $306,000 for Dr. Atay, $306,000 for Mr. Taylor, and $270,300 for Ms. Johnson. Effective from July 1, 2024, each of Dr. Atay and Mr. Taylor became eligible to be considered for a discretionary annual incentive bonus, subject to their continued employment through the date that bonuses are paid. Equity compensation Historically, the equity compensation granted to our named executive officers has consisted of stock options. For a description of the stock options granted to our named executive officers in fiscal year 2024, please see the “Outstanding equity awards at 2024 fiscal year-end”table below. 200

Employee benefits and perquisites Our named executive officers are eligible to participate in our health and welfare plans to the same extent as are full-time employees generally. We generally do not provide our named executive officers with perquisites or other personal benefits. Retirement benefits We have established a 401(k) tax-deferredsavings plan, which permits participants, including our named