Company: NCEL
Filing Date: 2025-06-09
Form Type: F-4/A
Source: 0001213900-25-052354
Chunk: 357

Company: NewcelX Ltd.
Filing Date: 2025-06-09
Form: F-4/A
Chunk 357
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 scopolamine -inducedamnesia. In a preclinical study to test the effects of NLS -8on memory in a model of Alzheimer’s Disease, the scopolamine -inducedamnesia in the novel object recognition test in mice. C57BL/6 male mice, 6 groups (N=16 mice/group), were subjected to two 12 -mintrials, 90 -minapart, in the NOR test: a sample (acquisition) trial during which they were exposed to two identical objects, and a choice (retention) trial during which they were exposed to two different objects presented, the familiar object (presented at the sample trial), and the novel object. They received 30 min before the sample trial i.p. injections of vehicle (control group), of scopolamine, of scopolamine + donepezil (1 mg/kg) or of 187 scopolamine + NLS -8(50, 100 or 150 mg/kg). Scopolamine was injected at 1.2 mg/kg. The results of this pre -clinicalstudy showed that NLS -8improved amnesia induced by scopolamine suggesting that NLS -8may improve memory and reduce cognitive symptoms of AD. These effects were significant at doses of 50 and 150 mg/kg and was close to significant at the dose of 100 mg/kg. NLS-11 NLS -11(Benedin) (formerly SCH -5472) is a norepinephrine and dopaminereuptake inhibitor and muscarinic M1, M2, M3 receptor antagonist. In a preclinical study, 57BL/6 male mice, 8 groups (N = 16 mice/group), were subjected to the effect of NLS -11(0.1, 0.5, 1 mg/kg) and compared to those of vehicle and of donepezil (2 mg/kg). Long -termepisodic memory was tested in the NOR test, using a 3 -daysinterval between the acquisition session (called sample trial) and the retention session (called choice trial). The results of the study showed NLS -11improved the recognition of the familiar object, i.e. improved memory and suggest that NLS -11induced a long -termmemory improvement which was significant and of the same extent to that induced by donepezil. At doses tested, NLS -11did not reduce exploratory behavior, contrary to donepez