ReviewAnon/tilebac-sample-stitched-montage

Montage images were automatically collected on Thermo Fisher Scientific Krios G4 in nanoprobe TEM mode with 70 μm C2 aperture under cryogenic conditions. The Falcon 3EC direct electron detector (counting mode) with a total electron dose of 4 e-/Å2 per montage image. For montage image automation, an automated montage search map was collected using Tomography 5 (Thermo Fisher Scientific) 7 × 7 montage tiles at 4096 × 4096 image size (2.128 nm/pix). These parameters were chosen to cover the space of a grid square for a 200-mesh size grid.