verdict string | claim_text string | confidence float64 | protocol_text string | evidence_spans list | protocol_steps list | protocol_category string | protocol_page_html string | verification_reason string | experimental_subjects list | experimental_conditions dict |
|---|---|---|---|---|---|---|---|---|---|---|
无法判断 | 该协议能够证明所有在选择培养基上生长的菌落都表达GFP。 | 0.94 | 将酿酒酵母感受态细胞与含GFP基因的质粒DNA在冰上孵育10分钟。
随后进行热激处理，转入YPD恢复液中，在30°C培养45分钟。
将细胞涂布于缺失相应营养物的选择培养基上，30°C静置培养48小时。
协议仅规定通过营养缺陷互补筛选转化子，未规定对每个菌落进行荧光检测。 | [
{
"quote": "协议仅规定通过营养缺陷互补筛选转化子，未规定对每个菌落进行荧光检测。",
"relation": "中性",
"start_offset": 101,
"end_offset": 135
}
] | [
"将酿酒酵母感受态细胞与含GFP基因的质粒DNA在冰上孵育10分钟。",
"进行热激处理并转入YPD恢复液中。",
"在30°C培养45分钟以完成恢复。",
"将细胞涂布于缺失相应营养物的选择培养基上。",
"在30°C静置培养48小时并通过营养缺陷互补筛选转化子。"
] | 遗传实验 | <article><h1>酿酒酵母质粒转化协议</h1><p>将酿酒酵母感受态细胞与含GFP基因的质粒DNA在冰上孵育10分钟。</p><p>随后进行热激处理，转入YPD恢复液中，在30°C培养45分钟。</p><p>将细胞涂布于缺失相应营养物的选择培养基上，30°C静置培养48小时。</p><p>协议仅规定通过营养缺陷互补筛选转化子，未规定对每个菌落进行荧光检测。</p></article> | 选择培养基上的生长只能说明协议设置了营养缺陷互补筛选，正文没有规定逐菌落进行GFP荧光检测，也没有提供表达结果，因此无法据此证明所有生长菌落都表达GFP。 | [
"酿酒酵母感受态细胞",
"含GFP基因的质粒DNA",
"YPD恢复液",
"营养缺陷选择培养基"
] | {
"temperature": 30,
"temperature_unit": "摄氏度",
"duration": 48,
"duration_unit": "小时",
"culture_medium": "YPD恢复液和缺失相应营养物的选择培养基",
"gas_condition": "未说明",
"other_constraints": [
"质粒DNA与感受态细胞在冰上孵育10分钟",
"热激后在YPD恢复液中恢复45分钟",
"通过营养缺陷互补进行筛选",
"未规定对每个菌落进行荧光检测"
]
} |
支持 | 该细胞培养协议要求在37°C和5% CO2条件下培养24小时。 | 0.99 | 将贴壁型哺乳动物细胞接种于含10%胎牛血清的DMEM培养基中。
置于37°C、5% CO2和饱和湿度培养箱内培养24小时。
细胞达到约80%融合度后，用PBS清洗一次，再用胰蛋白酶消化并传代。
该流程用于维持细胞增殖，不包含药物处理或终点活性测定。 | [
{
"quote": "置于37°C、5% CO2和饱和湿度培养箱内培养24小时。",
"relation": "支持",
"start_offset": 32,
"end_offset": 61
}
] | [
"将贴壁型哺乳动物细胞接种于含10%胎牛血清的DMEM培养基中。",
"在37°C、5% CO2和饱和湿度条件下培养24小时。",
"细胞达到约80%融合度后用PBS清洗一次。",
"使用胰蛋白酶消化细胞并进行传代。"
] | 细胞培养 | <article><h1>细胞培养协议</h1><p>将贴壁型哺乳动物细胞接种于含10%胎牛血清的DMEM培养基中。</p><p>置于37°C、5% CO2和饱和湿度培养箱内培养24小时。</p><p>细胞达到约80%融合度后，用PBS清洗一次，再用胰蛋白酶消化并传代。</p><p>该流程用于维持细胞增殖，不包含药物处理或终点活性测定。</p></article> | 协议正文明确给出了37°C、5% CO2和24小时培养时长，与待验证主张中的全部关键条件一致。 | [
"贴壁型哺乳动物细胞",
"DMEM培养基",
"胰蛋白酶"
] | {
"temperature": 37,
"temperature_unit": "摄氏度",
"duration": 24,
"duration_unit": "小时",
"culture_medium": "含10%胎牛血清的DMEM培养基",
"gas_condition": "5% CO2、饱和湿度",
"other_constraints": [
"细胞达到约80%融合度后再传代",
"传代前用PBS清洗一次",
"不进行药物处理或终点活性测定"
]
} |
反驳 | 该协议在厌氧条件下培养大肠杆菌K-12 8小时。 | 0.99 | 将大肠杆菌K-12单菌落接种到LB液体培养基中。
在37°C、180 rpm振荡条件下培养8小时，培养过程中保持有氧通气。
培养结束后取样测定OD600，不将OD600读数解释为菌落形成单位。
该协议用于获得对数末期附近的培养物。 | [
{
"quote": "在37°C、180 rpm振荡条件下培养8小时，培养过程中保持有氧通气。",
"relation": "反驳",
"start_offset": 25,
"end_offset": 61
}
] | [
"将大肠杆菌K-12单菌落接种到LB液体培养基中。",
"在37°C、180 rpm振荡条件下培养8小时。",
"培养过程中保持有氧通气。",
"培养结束后取样测定OD600。",
"不将OD600读数解释为菌落形成单位。"
] | 微生物培养 | <article><h1>大肠杆菌培养协议</h1><p>将大肠杆菌K-12单菌落接种到LB液体培养基中。</p><p>在37°C、180 rpm振荡条件下培养8小时，培养过程中保持有氧通气。</p><p>培养结束后取样测定OD600，不将OD600读数解释为菌落形成单位。</p><p>该协议用于获得对数末期附近的培养物。</p></article> | 协议明确规定培养过程中保持有氧通气，因此与待验证主张所称的厌氧条件相矛盾；8小时的培养时长虽然一致，但不能改变气体条件相反这一事实。 | [
"大肠杆菌K-12",
"LB液体培养基"
] | {
"temperature": 37,
"temperature_unit": "摄氏度",
"duration": 8,
"duration_unit": "小时",
"culture_medium": "LB液体培养基",
"gas_condition": "有氧通气",
"other_constraints": [
"振荡速度为180 rpm",
"培养结束后测定OD600",
"OD600不解释为菌落形成单位"
]
} |
Virtual Biological Experiment Protocol Fact Verification Dataset
This dataset brings together protocols from virtual biological experiment webpages, covering sample handling, cell or microbial culture, genetic experiments, ecological observation, and testing. Records organize source HTML, cleaned protocol text, experimental conditions, and procedural steps, and may include claims about experimental conditions, biological processes, or results paired with evidence spans, verdicts, confidence scores, and explanations. It supports training and evaluating models for evidence retrieval, claim verification, and reasoning over constraints in specialized biological web content.
Technical Specifications
| Field | Type | Description |
|---|---|---|
| verdict | string | The factual judgment made about the claim based on the experimental protocol text and evidence spans. |
| claim_text | string | A claim about experimental conditions, biological processes, or experimental results that must be checked against the protocol text. |
| confidence | number | The confidence score for the fact verification verdict, ranging from 0 to 1. |
| protocol_text | string | Protocol text extracted from the source webpage and cleaned for use. |
| evidence_spans | array | Source text excerpts and their locations that support, refute, or do not directly resolve the claim. |
| protocol_steps | array | Protocol operations organized in the order in which the experiment is performed. |
| protocol_category | string | The primary biological experiment category identified from the protocol content. |
| protocol_page_html | string | The original webpage HTML containing sample handling, culture, genetic experiment, ecological observation, or testing protocols. |
| verification_reason | string | An explanation of the verdict based on experimental conditions, biological processes, and evidence spans. |
| experimental_subjects | array | Samples, cells, microorganisms, genes, or ecological subjects extracted from the protocol. |
| experimental_conditions | object | Conditions and constraints extracted from the protocol, such as temperature, duration, culture medium, and gas environment. |
Compliance Statement
| Authorization Type | CC-BY-NC-SA 4.0 (Attribution–NonCommercial–ShareAlike) |
| Commercial Use | Requires exclusive subscription or authorization contract (monthly or per-invocation charging) |
| Privacy and Anonymization | No PII, no real company names, simulated scenarios follow industry standards |
| Compliance System | Compliant with China's Data Security Law / EU GDPR / supports enterprise data access logs |
Source & Contact
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