Document Classification

Section Type: METHODS

Text: After stabilization, the muscle slices were randomized into five experimental groups (n = 6/group) : 1) aerobic control-incubated in oxygenated buffer for 210 min, 2) ischemia alone-90 min ischemia followed by 120 min reoxygenation, 3) preconditioning by 5 min ischemia/5 min reoxygenation before 90 min ischemia/120 min reoxygenation, 4) diazoxide (Mito KATP opener, 0. 1 mm) - for 10 min before the 90 min ischemia/120 min reoxygenation and 5) glibenclamide (10 microm) -10 min exposure prior to PC (only in the diabetic patient groups).