Document Classification

Section Type: METHODS

Text: Before and after treatment, the following parameters in blood were detected : the peripheral Treg, percentage (CD19 +), activation rate (CD19 + CD69 +) and apoptotic rate (AV) of B-lymphocyte by flow cytometry ; cytokines (IL-6 and IL-10) by CBA stream protein analyzing system ; high sensitivity C-reactive protein (hs-CRP) by scattering turbidimetric analysis ; homocysteine (Hcy) by end-point method ; hemoglobin (HGB) content by Beckman-Coulter hemo-analyser ; blood contents of Ca, phosphate (P), blood urea nitrogen (BUN), creatinine (SCr) and plasma albumin (Alb) by automatic biochemical analyser ; and urinary contents of creatinine (UCr) by inverse HPLC.