Patent Number: 
Section: claims

1. A method for radiographic density evaluation of a material, said method comprising:capturing a radiographic image of the material with a cassette by projecting X-rays from a source through the material, the cassette and/or software is configured to obtain information to perform intensity normalization and standardization of the radiographic image;performing intensity standardization of the radiographic image;analyzing the radiographic image to evaluate radiographic tissue density of the biological material; andwherein the intensity standardization is performed comprises estimating a background, the background created by an instrument source-detector geometry and baseline responses; and subtracting the background from the radiographic image, and converting the radiographic image from a grayscale image into color-coded intensity images that form an intuitive colormap based on a calibration bar with a predetermined radiographic signature on the cassette to serve as reference for performing the intensity standardization. 2. The method of claim 1 wherein the source is an X-ray radiography machine. 3. The method of claim 1 wherein the cassette is placed under the subject relative to a direction of the source. 4. The method of claim 1 further comprising diagnosing a disease or condition in the biological material when the radiographic tissue density is outside of a preselected threshold. 5. The method of claim 1 wherein the cassette is configured for use with the source producing X-ray exposures of varying kVp, mAs and time. 6. The method of claim 1 wherein the cassette further comprises a radio-opaque backing having an X-ray radiographic signature and estimating a source-detector geometrical inhomogeneity, and placing at least one calibration bar or other device with a predetermined radiographic signature on the cassette to serve as references for performing the intensity standardization. 7. The method of claim 1 further comprising placing at least one calibration bar with a predetermined radiographic signature on or within the cassette, the at least one calibration bar configured to have a graduated radio-opacity inset. 8. The method of claim 1 wherein the material is a living human subject. 9. The method of claim 1 wherein the material is a biological tissue or organ or organelle. 10. The method of claim 1 wherein the material is a non-biological. 11. The method of claim 1 wherein the source is an X-ray microscope.