Patent Number: 
Section: claims

1. A system for independently holding and manipulating a plurality of microscopic objects and for targeting at least a part of one or more microscopic objects within a trapping volume with electromagnetic radiation, the system comprising:an optical trapping system for holding and manipulating the plurality of microscopic objects comprising:a trapping electromagnetic radiation source for emitting trapping electromagnetic radiation,a secondary spatial electromagnetic radiation modulator for receiving and spatially shaping the trapping electromagnetic radiation so as to generate modulated trapping electromagnetic radiation which may be directed towards the plurality of microscopic objects,an electromagnetic radiation generator, the electromagnetic radiation generator comprising:a targeting electromagnetic radiation source for emitting targeting electromagnetic radiation, anda primary spatial electromagnetic radiation modulator for receiving and spatially shaping the targeting electromagnetic radiation so as to generate modulated targeting electromagnetic radiation directed towards at least a part of the plurality of microscopic objects so as to enable specifically targeting at least a part of the plurality of microscopic objects within the trapping volume,wherein the optical trapping system and the electromagnetic radiation generator are enabled to function independently of each other, and wherein the electromagnetic radiation generator enables independent and simultaneous targeting of at least two spatially different microscopic objects, and wherein the optical trapping system and the electromagnetic radiation generator are spatially separated, and wherein the electromagnetic radiation generator is not able to overcome the forces applied by the optical trapping system so that a microscopic object, which is trapped by the optical trapping system, will remain trapped regardless of the actions of the electromagnetic radiation generator, and wherein the modulated targeting electromagnetic radiation is not able to optically trap the microscopic objects. 2. The system according to claim 1, wherein the optical trapping system is enabling trapping at least two spatially different microscopic objects, wherein the spatially different microscopic objects may be positioned at spatially different planes with respect to an optical axis of the trapping means. 3. The system according to claim 1, wherein the electromagnetic radiation generator is enables targeting or focusing on, at least two spatially different microscopic objects, wherein the spatially different microscopic objects may be positioned at spatially different planes with respect to an optical axis of the electromagnetic radiation generator. 4. The system according to claim 1, wherein the optical trapping system enables independently trapping at least 100 microscopic objects. 5. The system according to claim 1, wherein the electromagnetic generator enables independently targeting at least 100 microscopic objects. 6. The system according to claim 1, wherein the modulated trapping electromagnetic radiation and the modulated targeting electromagnetic radiation have different wavelengths or the wavelength of the modulated trapping electromagnetic radiation is 1064 nm and the wavelength of the modulated targeting electromagnetic radiation is 532 nm. 7. The system according to claim 1, wherein the optical trapping system comprises a trapping spatio-temporal unit enabling varying of the position of the plurality of microscopic objects, and wherein the trapping spatio-temporal unit and the primary electromagnetic radiation modulator are spatially separated. 8. The system according to claim 1, wherein the primary spatial electromagnetic radiation modulator and the secondary spatial electromagnetic radiation modulator are physically separated. 9. The system according to claim 1, wherein the primary spatial electromagnetic radiation modulator is arranged for applying a spatial modulation of the targeting electromagnetic radiation by changing its properties locally. 10. The system according to claim 1, wherein the secondary spatial electromagnetic radiation modulator is arranged for applying a spatial modulation of the trapping electromagnetic radiation by changing its properties locally. 11. The system according to claim 2, wherein the position of at least one of the planes with respect to an optical axis of the optical trapping system may be changed. 12. The system according to claim 3, wherein the position of at least one of the planes with respect to an optical axis of the electromagnetic radiation generator may be changed. 13. The system according to claim 12, wherein the position of at least one of the planes with respect to an optical axis may be changed so as to move from one side of a microscopic object being trapped by the optical trapping system to the other side of a microscopic object being trapped by the optical trapping system along an optical axis of the electromagnetic radiation generator. 14. The system according to claim 1, further comprising:a sensor that determines the position and/or orientation, of the plurality of microscopic objects. 15. The system according to claim 1, wherein the system further comprises one or more micro devices, wherein the one or more micro devices:are arranged for spatial manipulation by the optical trapping system or the optical trapping system enables control over translational movement in three dimensions and rotational movement around at least two axes of the one or more micro devices, andare arranged for receiving the modulated targeting electromagnetic radiation and furthermore shaping, focusing, redirecting the modulated targeting electromagnetic radiation and/or changing the modulated targeting electromagnetic radiation from farfield to nearfield. 16. The system according to claim 1, wherein the system further comprises one or more micro devices and, wherein the one or more micro devices are each arranged for holding a microscopic optical element or a spherical bead. 17. The system according to claim 1, wherein the system further comprises:an electromagnetic radiation detector arranged for receiving electromagnetic radiation emitted from within the trapping volume, or emitted from the one or more microscopic objects. 18. The system according to claim 1, wherein any one of the optical trapping system and/or the electromagnetic radiation generator comprises a setup relying on a Generalized Phase Contrast. 19. A method for independently holding and/or manipulating one or more microscopic objects and for targeting at least a part of the one or more microscopic objects within a trapping volume with electromagnetic radiation, the method comprising:trapping the one or more microscopic objects within the trapping volume by using optical trapping system, comprising:a trapping electromagnetic radiation source for emitting trapping electromagnetic radiation,a secondary spatial electromagnetic radiation modulator for receiving and spatially shaping the trapping electromagnetic radiation so as to generate modulated trapping electromagnetic radiation, which may be directed towards the plurality of microscopic objects,receiving and spatially shaping targeting electromagnetic radiation using an electromagnetic radiation generator, the electromagnetic radiation generator comprising:a targeting electromagnetic radiation source for emitting targeting electromagnetic radiation,a primary spatial electromagnetic radiation modulator for receiving and spatially shaping the targeting electromagnetic radiation so as to generate modulated targeting electromagnetic radiation directed towards at least a part of the plurality of microscopic objects so as to enable specifically targeting at least a part of the plurality of microscopic objects within the trapping volume,so as to generate modulated targeting electromagnetic radiation directed towards at least a part of the one or more microscopic objects so as to specifically target at least a part of the one or more microscopic objects within the trapping volume by using an electromagnetic radiation generator,wherein the optical trapping system and the electromagnetic radiation generator are enabled to function independently of each other, and wherein the electromagnetic radiation generator enables independently and simultaneously targeting of at least two spatially different microscopic objects, and wherein the optical trapping system and the electromagnetic radiation generator are spatially separated, and wherein the electromagnetic radiation generator is not able to overcome the forces applied by the optical trapping system so that a microscopic object which is trapped by the optical trapping system will remain trapped regardless of the actions of the electromagnetic radiation generator, and wherein the modulated targeting electromagnetic radiation is not able to optically trap the microscopic objects.