Patent Number: 
Section: claims

1. A manipulator for handling micron-scale samples, the manipulator comprising:a probe includingan electrically conductive surface,a proximal end and a distal end, the distal end having a truncated conical shape with a generally flat face, the face having a diameter along a minor axis of less than about 50 μm, anda bore extending from the proximal end to the face;a probe holder engaged with the proximal end of the probe and including a first inlet in fluid communication with the bore and with a source of sub-ambient pressure; anda mechanical positioner configured to cause and control micron-scale movements of the probe holder. 2. The manipulator of claim 1 wherein the probe holder further comprises a second inlet in fluid communication with the bore and with a source of ambient or super-ambient pressure. 3. The manipulator of claim 1 wherein the probe comprises a glass capillary tube. 4. The manipulator of claim 3 wherein the glass capillary tube comprises glass having a dielectric constant less than 10. 5. The manipulator of claim 1 wherein the electrically conductive surface comprises a metal coating. 6. The manipulator of claim 5 wherein the metal coating includes chromium. 7. The manipulator of claim 1 wherein the probe defines a longitudinal axis, the generally flat face defines a plane, and a normal axis of the plane is angled with respect to the longitudinal axis. 8. The manipulator of claim 7 wherein the normal axis of the plane is angled with respect to the longitudinal axis by an angle in the range of 30° to 65°. 9. The manipulator of claim 1 wherein a diameter of the bore at the distal end is about 5 μm or less. 10. The manipulator of claim 1 wherein the probe holder includes a path to an electrical ground in order to ground the probe. 11. A manipulator system for handling micron-scale samples, the system comprising:a probe includingan electrically conductive surface,a proximal end and a distal end, the distal end having a truncated conical shape with a generally flat face, the face having a diameter along a minor axis of less than about 50 μm, anda bore extending from the proximal end to the face;a probe holder engaged with the proximal end of the probe and including a first inlet in fluid communication with the bore;a mechanical positioner configured to cause and control micron-scale movements of the probe holder; anda source of sub-ambient pressure coupled to the inlet. 12. The manipulator system of claim 11 further comprising a source of ambient or super ambient pressure coupled to a second inlet in fluid communication with the bore. 13. A method for microscopy inspection, comprising:forming a free-standing membrane;positioning a probe proximate to the membrane, the probe includingan electrically conductive surface,a distal end having a truncated conical shape with a generally flat face, the face having a diameter along a minor axis of less than about 50 μm,a bore extending from the proximal end to the face, anda probe holder engaged with the proximal end of the probe and including a first inlet in fluid communication with the bore;drawing a vacuum through the bore to draw the membrane to the face;placing the membrane on a sample support; andinspecting the membrane with a microscope. 14. The method of claim 13 wherein forming the free-standing membrane includes focused ion beam milling. 15. The method of claim 13 wherein positioning the probe includes translating a probe holder engaged with the probe. 16. The method of claim 13 wherein placing the membrane includesrepositioning the probe such that the membrane is disposed proximate to the sample support; anddiscontinuing to draw the vacuum through the bore. 17. The method of claim 16 wherein the sample support is a TEM support grid. 18. The method of claim 16 further comprising introducing ambient or super-ambient pressure through the bore. 19. The method of claim 16 further comprising repositioning the probe such that the membrane touches the sample support. 20. The method of claim 13 wherein forming the free-standing membrane and placing the membrane on the sample support are performed within a focused ion beam milling system.