Patent Number: 039742695
Section: claims

1. A radioimmunoassay method for determining the presence of Neisserria gonorrhoeae antibodies in human serum which comprises the steps of: A. adding anti-human IgG to the serum to be tested in a buffered aqueous medium,  B. thereafter adding a heat labile antigen which has been produced by a growth culture of Neisseria gonorrhoeae, isolated therefrom, and labelled with a detectable radioactive element,  C. incubating resulting mixture at from about 4.degree.C to 45.degree.C for from about 24 to 2 hours at a pH of from about 6.5 to 8.5 to form an antigen-antibody conjugate when said antibodies are present, and  D. determining the level of radioactivity as a measure of the presence of said antigen-antibody conjugate.  A. adding anti-human IgG to the serum to be tested in a buffered aqueous medium,  B. thereafter adding a heat labile antigen which has been produced by a growth culture of Neisseria gonorrhoeae, isolated therefrom, and labelled with a detectable radioactive element,  C. incubating resulting mixture at from about 4.degree.C to 45.degree.C for from about 24 to 2 hours at a pH of from about 6.5 to 8.5 to form an antigen-antibody conjugate when said antibodies are present,  D. filter resulting mixture through a filter which has been previously washed with a reagent selected from the group consisting of bovine serum albumin, human serum immunoglobulin, ovalbumin, and hemoglobin to separate said antigen-antibody conjugate, and  E. determining the level of radioactivity in resulting precipitate as a measure of the presence of said antigen-antibody conjugate.  A. adding anti-human IgG to the serum to be tested in a buffered aqueous medium containing a reagent selected from the group consisting of bovine serum albumin, human serum immunoglobulin, ovalbumin, and hemoglobin,  B. thereafter adding a heat labile antigen which has been produced by a growth culture of Neisseria gonorrhoeae, isolated therefrom, and labelled with a detectable radioactive element together with bovine serum albumin,  C. incubating resulting mixture at from about 4.degree.C to 45.degree.C for from about 24 to 2 hours at a pH of from about 6.5 to 8.5 to form an antigen-antibody conjugate when said antibodies are present,  D. diluting resulting mixture with an aqueous buffer and centrifuging, and  E. determining the level of radioactivity in resulting precipitate as a measure of the presence of said antigen-antibody conjugate. 2. A method as in claim 1 wherein the radioactive element is .sup.125 I. 3. A radioimmunoassay method for determining the presence of Niesseria gonorrhoeae antibodies in human serum which comprises the steps of: 4. A method as in claim 3 wherein the radioactive element is .sup.125 I. 5. A method as in claim 3 wherein the reagent utilized in Step D is bovine serum albumin. 6. A method as in claim 3 wherein the radioactive element is .sup.125 I and the reagent utilized in Step D is bovine serum albumin. 7. A radioimmunoassay method for determining the presence of Neisseria gonorrhoeae antibodies in human serum which comprises the steps of: 8. A method as in claim 7 wherein the reagent is bovine serum albumin. 9. A method as in claim 7 wherein the radioactive element is .sup.125 I. 10. A method as in claim 7 wherein the buffer is phosphate buffered saline. 11. A method as in claim 7 wherein the reagent is bovine serum albumin, the radioactive element is .sup.125 I and the buffer is phosphate buffered saline.