Patent Number: 
Section: claims

1. A microscope examination method comprising:a step of introducing into a specimen a substance in which a structural change is caused by irradiation with light of a first wavelength;a step of specifying an optical stimulation site in the specimen by irradiating the specimen with visible light of a second wavelength that does not cause a structural change in the substance, while examining the specimen in which the substance is introduced using a microscope examination apparatus; anda step of irradiating the specified optical stimulus site with the light of the first wavelength. 2. The microscope examination method according to claim 1, wherein the microscope examination apparatus is formed of a fluorescence microscope that irradiates the specimen with excitation light of a third wavelength different from the first wavelength and the second wavelength to observe the fluorescence produced thereby. 3. A microscope examination method according to claim 1, wherein the microscope examination apparatus is a stereomicroscope. 4. An optical stimulation apparatus comprising:a first light source that emits light of a first wavelength that irradiates a substance introduced into a specimen to cause a structural change thereof;a second light source that emits visible light of a second wavelength, different from the first wavelength, the second wavelength not causing a structural change in the substance;an optical-axis combining unit that aligns the optical axis of the first light source and the optical axis of the second light source; anda focusing optical system that focuses the light from the first and second light sources, wherein the focusing optical system is removably attached to the optical-axis combining unit. 5. An optical stimulation apparatus according to claim 4, further comprising:optical fibers that connect the optical-axis combining unit with the first and second light sources, respectively; andfocus adjusting mechanisms that can adjust the tip positions of the optical fibers at the connection points between the optical-axis combining unit and the optical fibers. 6. An optical stimulation apparatus according to claim 4, further comprising:an optical fiber that guides the light beams whose optical axes are aligned by the optical-axis combining unit to the focusing optical system;a focus adjusting mechanism that can adjust the distance between an exit face of the optical fiber and each light source; anda chromatic-aberration correcting member, provided in the focusing optical system, for correcting aberration of the light of the first wavelength and the second wavelength. 7. A microscope examination apparatus including the optical stimulation apparatus according to claim 4, the microscope examination apparatus comprising:an objective optical system disposed adjacent to the specimen;an illumination optical system that introduces illumination light to the objective optical system; andan eyepiece optical system that allows observation of return light from the specimen;wherein the objective optical system is provided with:a light introducing member that introduces light from the first and second light sources whose optical axes are aligned;an aperture that limits the image of the first and second light sources formed on the specimen; andan aperture moving mechanism that moves the aperture in directions orthogonal to the optical axis.