Patent Number: 
Section: claims

1. A method of constructing a 4D tilt series of images of a moving object, the method comprising:(a) irradiating the moving object with an optical pulse;(b) directing an electron pulse to impinge on the moving object;(c) detecting the electron pulse;(d) processing the detected electron pulse to form an image of the moving object;(e) capturing the image of the moving object associated with a time step; and(f) repeating (a)-(e) to construct the 4D tilt series of images of the moving object. 2. The method of claim 1 wherein the time step is on a scale of nanoseconds. 3. The method of claim 1 wherein the electron pulse consists of a single electron. 4. The method of claim 1 wherein the electron pulse comprises a plurality of electrons. 5. The method of claim 1 wherein the moving object comprises a nanomechanical cantilever. 6. The method of claim 1 wherein the moving object comprises a biological entity selected from at least one of a cell, a protein, a nucleic acid, a virus, or a complex thereof. 7. The method of claim 1 wherein the 4D tilt series of images is a tomographic tilt series of images capturing a portion of the moving object. 8. The method of claim 1 further comprising measuring a difference between each image in the 4D tilt series of images at a microscale level. 9. The method of claim 1, further comprising measuring a difference between each image in the 4D tilt series of images at a nanoscale level.