Patent Number: 
Section: claims

1. A micro-manipulation method for manipulating a micro-object with a micro-handling tool under electron beam irradiation of an electron microscope, wherein said method comprises steps of: adjusting accelerating voltage of the electron beam, a first potential of the micro-handling tool and a second potential of a work substrate; and  picking up and releasing the micro-object with the micro-handling tool. 2. A micro-manipulation method according to  claim 1 , wherein said micro-object has a predetermined size, and the accelerating voltage of the electron beam is adjusted to make the electron beam penetrate the micro-object in the predetermined size. claim 1 3. A micro-manipulation method according to  claim 1  or  2 , said method further comprises a step of: claim 1 2 preparing the work substrate by providing a conductive glass substrate and coating a polymer film on the conductive glass substrate, whereby the adhesion force between the micro-object and the substrate is increased. 4. A micro-manipulation method according to  claim 1  or  2 , said method further comprises a step of: claim 1 2 preparing the work substrate by providing a glass substrate, evaporating an ITO film of a transparent conductive electrode on the glass substrate, and dip-coating a polystyrene film, whereby increasing an adhesion force between the micro-object and the work substrate.