PATENT DOCUMENT

Abstract:
The present invention provides a novel DNA microarray chip that can be used for simultaneous testing of transcriptional responses to cutaneous stressors in the context of neuro-endocrine-immune functions of the skin. The transcriptional responses to ultraviolet radiation in epidermal keratinocytes were tested using such microarray chip containing more than 700 neuro-endocrine-immune related genes. The gene expression pattern was non-random and time dependent; it included increased expression of genes involved in water and salt balance, prostaglandin synthesis, keratinocyte differentiation as well as genes coding for stress effectors, cytokines and metalloproteinases. In contrast, expression was decreased for genes coding for growth factors and their receptors, and for elements of extracellular matrix. This stochastic pattern suggests that transcriptional responses are coordinated and aimed at preservation of epidermal barrier function, prevention of early carcinogenic events and remodeling of extracellular matrix.

Full Description:
CROSS-REFERENCE TO RELATED APPLICATION  
       [0001]     This non-provisional application claims benefit of provisional patent applications Ser. No. 60/592,875, filed Jul. 30, 2004, now abandoned. 
     
    
     FEDERAL FUNDING LEGEND  
       [0002]     This invention was produced in part using funds obtained through a grant (AR047079-01A2) from the National Institutes of Health. Consequently, the federal government has certain rights in this invention. 
     
    
     BACKGROUND OF THE INVENTION  
       [0003]     1. Field of the Invention  
         [0004]     The present invention relates generally to the molecular biology and genomics technology. More specifically, the present invention relates to microarrays for the investigation of skin neuro-endocrine-immune functions.  
         [0005]     2. Description of the Related Art  
         [0006]     As a biological barrier, the skin is continuously subjected to the environmental stresses such as ultraviolet radiation, pathogens and thermal and chemical insults (Slominski and Wortsman, 2000). To provide systemic protection, skin cells have numerous defense mechanisms, presumably evolving from a network of intracellular communications coordinated by messages from the local neuro-endocrine-immune system (Slominski and Wortsman, 2000).  
         [0007]     This concept, of skin as a peripheral neuroendocrine organ, would imply that local homeostasis (and by inference systemic homeostasis) is regulated by the expression of hormones, neurotransmitters, neuropeptides, cytokines and their receptors. Accordingly, expression of the respective genes should be hierarchical, coordinated and organized into patterns defining functional pathways after exposure to environmental stressors and/or pathological events. The predominant environmental stressor for the skin is solar radiation, especially its UV wavelengths (200-320 nm) (Slominski and Pawelek, 1998). Primary responses to UV radiation (UVR) involve induction of melanin pigmentation and immunomodulation and endocrine activity represented by production of vitamin D and neuropeptides (Slominski and Pawelek, 1998; Slominski and Wortsman, 2000).  
         [0008]     Chronic UVR exposure has potent carcinogenic and aging effects. However, the prior art is deficient in the development of a microarray assay to obtain further insight into the cutaneous stress response under chronic UVR exposure. The present invention fulfills this long-standing need and desire in the art by developing microarrays that could be used for simultaneous testing of transcriptional responses to cutaneous stressors in context of neuroendocrine-immune functions of the skin.  
       SUMMARY OF THE INVENTION  
       [0009]     The present invention is directed to a microarray comprising a human molecular chip composed of approximately 700 genes developed to study peripheral response to stress. The chip was designed to evaluate hierarchical and coordinated gene expression patterns organized into functional pathways in response to environmental stress, dyshomeostatic stimuli or pathology. These human microarrays included oligonucleotides corresponding to genes encoding enzymes involved in hormonal production or degradation or neuropeptide processing, precursors to neuropeptide, cytokines, corresponding receptors, other immune markers. For completeness of such molecular chips, several structural genes governing melanogenesis (specific for melanocytes) or epidermal barrier formation by keratinocytes (different cytokeratins) were also included to allow evaluation of the neuroendocrine and immune pathways in relation to differentiated state of prevalent cells of the epidermal component. The oligonucleotides for genes in the microarray chip not limited to include the ones shown in Table 1.  
         [0010]     This technology was then applied to the testing of transcriptional activity in keratinocytes exposed to UVR. Significant expression changes of genes arranged in patterns defining functional pathways were found. Thus, this chip could be used as an efficient molecular technology to study neuroendocrine and immune functions of the skin and other organs on a comprehensive molecular level in relation to external and internal stress or any dyshomeostatic signal. It would further allow shaping the modern concept of skin as a neuroendocrine organ requiring that local homeostasis and by inference systemic homeostasis is dependent on the regulated local expression of hormones, neurotransmitters, neuropeptides, cytokines and their receptors.  
         [0011]     In one aspect of the present invention, there is provided a DNA microarray chip for measuring transcriptional responses to stress in a cell. The chip comprises 761 oligonucleotides for genes related to production and metabolism of hormones, neurotransmitters, neuropeptides, cytokines, biological modulators, growth factors, corresponding receptors and normal skin metabolism genes.  
         [0012]     In another aspect of the present invention, there is provided a method of detecting transcriptional responses to stress in a cell that comprises following steps. The cell is challenged with stress and nucleic acid samples are isolated from the cell. This is followed by measuring gene expression levels of a group of genes. Subsequently, the gene expression levels are analyzed statistically and compared to those without stress, where a statistically significant upregulation of the gene expression indicates transcriptional responses to stress.  
         [0013]     In yet another aspect of the present invention, there is provided a method of detecting transcriptional responses to stress in a cell that comprises the same steps of challenging, isolating nucleic acid and measuring gene expression levels. However, in this case the gene expression levels are analyzed statistically and compared to those without stress, where a statistically significant downregulation of the gene expression indicates transcriptional responses to stress.  
         [0014]     Other and further aspects, features, and advantages of the present invention will be apparent from the following description of the presently preferred embodiment of the invention. This embodiment is given for the purpose of disclosure.  
     
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0015]     So that the matter in which the above-recited features, advantages and objects of the invention as well as others which will become clear are attained and can be understood in detail, more particular descriptions and certain embodiments of the invention briefly summarized above are illustrated in the appended drawings. These drawings form a part of the specification. It is to be noted, however, that the appended drawings illustrate preferred embodiments of the invention and therefore are not to be considered limiting in their scope.  
         [0016]      FIG. 1  shows detection of differentially expressed genes. Green dots represent genes with higher level of A546 labeled cDNA. Red dots represent genes with higher level of A647 labeled cDNA. Two green spots on the top are landing marks.  
         [0017]      FIGS. 2A and 2B  show relative expression of genes in UV-irradiated keratinocytes.  FIG. 2A  shows real-time RT-PCR results that were calculated by comparing UV-irradiated keratinocytes (incubated 12 hours after irradiation) against controls (no treatment). Data was normalized to GAPDH expression and represented mean ±SD (N=3). Ratios statistically significantly different from 1 (no difference) are denoted by asterisks (p&lt;0.05).  FIG. 2B  shows RT-PCR of human gastrin. The samples were loaded as follows: immortalized keratinocytes (HaCaT; lane 1); epidermal keratinocytes (HeKa; lane 2); epidermal fibroblasts (lane 3); different melanomas (lanes 4-9); melanocytes (lane 10). M denotes molecular marker. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION  
       [0018]     The following abbreviations are used herein: AQP, aquaporin; CYP4F8, cytochrome P450, subfamily IVF, polypeptide 8; COX-2, prostaglandin-endoperoxide synthase 2; IL-6, interleukin 6; IL-1, interleukin 1; IL-2, interleukin 2; IL-8, interleukin 8; TNFSF9, tumor necrosis factor superfamily member 9; BMP3, bone morphogenetic protein 3; IGFBP6, insulin-like growth factor binding protein 6; VGEF, vascular endothelial growth factor; EGFR, epidermal growth factor receptor; KGF, keratinocyte growth factor; KGFR, keratinocyte growth factor receptor; TNFSF10 (TRAIL), tumor necrosis factor superfamily member 10; MMPs, metalloproteinases; CRF-R2, corticotropin releasing factor receptor type 2.  
         [0019]     The present invention used microarray technology to detect several genes with changed expression levels (up- or down-regulated) in a time-dependent manner afetr exposure to UVR. Although the affected genes were mostly involved in water and salt balance and prostaglandin metabolism, they also included interleukins and growth factors, metalloproteinases, markers of keratinocyte differentiation and genes involved in stress response. Functional assessment revealed that majority of the detected genes represented defense mechanisms against malignant transformation. Thus, activation of interleukins, of keratinocyte differentiation markers and down-regulation of EGFR, KGF and KGFR may all represent anti-cancer defense mechanisms. In contrast, the stimulation of metalloproteinases, the enzymes responsible for the extracellular matrix rearrangement or the down-regulation of TRAIL might promote tumor progression in the human skin. Among all the genes that were analyzed, stimulation of COX-2, TNFSF9 and down-regulation of KGF and KGFR were the most interesting observation. Many of the gene expression changes induced by UVR have been already described. Thus, confirming the results obtained by using the microarray chip of the present invention with that already known made the data more reliable. The data was also verified independently using real-time PCR and sequencing. Thus, the microarray chip can be reliably used for the simultaneous assessment of the effect of different agents on the expression of a large set of endocrine-related genes.  
         [0020]     In one aspect of the present invention, there is provided a DNA microarray chip for measuring transcriptional responses to stress in a cell. The chip comprises 761 oligonucleotides for genes related to production and metabolism of hormones, neurotransmitters, neuropeptides, cytokines, biological modulators, growth factors, corresponding receptors and normal skin metabolism genes. The oligonucleotides for genes in the DNA microarray chip have SEQ ID Nos. shown in SEQ ID No. 25-785.  
         [0021]     In another aspect of the present invention, there is provided a method of detecting transcriptional responses to stress in a cell, comprising the steps of: challenging the cell with stress; isolating nucleic acid samples from the cell; measuring gene expression levels of a group of genes; and analyzing the gene expression levels statistically as compared to those without stress, wherein a statistically significant upregulation of the gene expression indicates transcriptional responses to stress. The gene expression is measured by DNA microarray and further verified by RT-PCR. Representative examples of the stress are ultraviolet radiation, thermal stress, chemical stress, or immune stress. The preferred cell is epidermal keratinocyte, melanocyte, fibroblast, or immune cell. Representative examples of the statistical analysis that can be performed are one-way analysis of variance, post-hoc test or student t test. The statistically significant upregulation is atleast a 1.5-fold increase of expression of the genes. The group of genes not limited to include genes involved in water and salt balance, genes involved in prostaglandin metabolism, interleukin genes, growth factor genes, markers of keratinocyte differentiation genes, metalloproteinases genes, and stress response genes. The upregulated genes not limited to include the genes selected from the group of genes encoding for gastrin, vasopressin, aquaporin, prostaglandin-endoperoxide synthase 2, CYP4F8, IL-1, IL-2, IL-6, IL-8, tumor necrosis factor superfamily member 9, bone morphogenetic protein 3, insulin-like growth factor binding protein 6, vascular endothelial growth factor B, stromelysins 1 and 2, corticotropin releasing factor receptor type 1 and 2, and aldo-keto reductase.  
         [0022]     In yet another aspect of the present invention, there is provided a method of detecting transcriptional responses to stress in a cell that comprises the same steps of challenging, isolating nucleic acid samples, measuring gene expression levels and statistical analysis of the gene expression levels as compared to those without stress as described supra. However in this case, a statistically significant downregulation of the gene expression indicates transcriptional responses to stress. All other aspects regarding the assay to measure gene expression and to verify its expression, the types of stress, type of cell and the genes whose expression is measured is the same as described supra. The down-regulated genes not limited to include genes selected from the group of genes encoding for epidermal growth factor receptor, keratinocyte growth factor, keratinocyte growth factor receptor, activin A receptor type I, and tumor necrosis factor superfamily member 10.  
         [0023]     The following examples are given for the purpose of illustrating various embodiments of the invention and are not meant to limit the present invention in any fashion. One skilled in the art will appreciate readily that the present invention is well adapted to carry out the objects and obtain the ends and advantages mentioned, as well as those objects, ends and advantages inherent herein. The present examples, along with the cells and methods described herein are presently representative of preferred embodiments, are exemplary, and are not intended as limitations on the scope of the invention. Changes therein and other uses which are encompassed within the spirit of the invention as defined by the scope of the claims will occur to those skilled in the art.  
       EXAMPLE 1  
       [0000]     Cell Lines and Cell Culture  
         [0024]     Human adult epidermal keratinocytes and melanocytes were purchased from Cascade Biologics and cultured according to the company&#39;s protocol (Cascade Bio, Portland, Oreg.). Melanocytes were grown in Medium 154 and keratinocytes in EpiLife medium supplemented with appropriate growth factors and antibiotics. For co-cultivation experiments, keratinocytes and melanocytes were mixed in the ratio 20:1 and incubated in Medium 154 supplemented with HKGS (Cascade Bio, Portland, Oreg.).  
       EXAMPLE 2  
       [0000]     Ultraviolet Light Treatment  
         [0025]     Ultraviolet irradiation of cells was performed as previously described (Pisarchik and Slominski, 2001). Briefly, cells were cultured in 75 cm 2  flasks at 85% confluency. The flasks were placed on the UV transilluminator 2000 (BioRad) and irradiated with 50 mJ/cm 2  UVB (Pisarchik and Slominski, 2001). Time of exposure, corresponding doses and UV spectrum had been established previously; after UV exposure (50 mJ/cm 2 ) the morphology of the cells did not change significantly (Pisarchik and Slominski, 2001). Irradiated cells were further incubated in culture media for 4, 12 and 24 hours, detached and processed for RNA isolation.  
       EXAMPLE 3  
       [0000]     Microarray Slides  
         [0026]     Oligonucleotides for genes related to production and metabolism of hormones, neurotransmiters, neuropeptides, cytokines, biological modulators, growth factors, corresponding receptors and normal skin metabolism genes were designed (http://www.utmem.edu/pathology/molecular-endocrinology.htm). The location of the oligos for each gene was chosen according to the structure of the corresponding gene obtained from the GeneBank. Oligonucleotides were synthesized by Integrated DNA Technologies (Coralville, Iowa) and printed on superamine slides (TeleChem, Sunnyvale, Calif.) at the University of Tennessee Molecular Resource Center.  
       EXAMPLE 4  
       [0000]     RNA extraction, cDNA Preparation and Fluorescent Labeling  
         [0027]     Total RNA was extracted using Trizol isolation kit (Gibco-BRL, Gaithersburg, Md.). The cDNA was synthesized, hybridized and labeled with Genisphere Array 50 kit according to the manufacturer protocol (Genisphere, Hatfield, Pa.).  
       EXAMPLE 5  
       [0000]     Hybridization Protocol  
         [0028]     Slides were washed by 0.2% SDS (2 min), water (two times, 2 min), dipped in absolute ethanol (1 min), dried by centrifugation (300×g, 2 min) followed by the injection of 100 μl of the hybridization mixture under the cover slip (LifterSlip cover glass, Erie Scientific Company, Portsmouth, N.H.). Slide hybridization and washing were done according to the protocol of the Genisphere Array 50 kit manufacture (Genisphere, Hatfield, Pa.). Slides were incubated overnight at 53° C. in the dark.  
       EXAMPLE 6  
       [0000]     Data Acquisition and Analysis  
         [0029]     At least four arrays were used in every experiment (every time-point). Fluorescence corresponding to A546 and A647 dyes was measured by Axon scanner at The University of Tennessee Molecular Resource Center. Program Fine PixPro 4.0 was used to identify spots and subtract background. Data was normalized by LOWES method (intensity-dependent) using the BRB-Array Tools 2.1 program written by Dr. Richard Simon (Biometrics Research Branch, National Cancer Institute) and Amy Peng (EMMES Corporation). The output Excel spreadsheet contained normalized logarithms (base 2) of ratios for each gene on the slide.  
         [0030]     For easy interpretation, logarithms were recalculated into ratios. The factor of 1.5 was chosen as the cut-off for increase/decrease of gene expression, instead of the generally-used factor of 2. This was done because some genes that did show statistically significant stimulation of expression did not attain the 2-fold difference. To avoid false positives, only those genes that were consistently up- or down-regulated on several slides including dye-flip slides as presented on  FIG. 1  were selected. Table 2 lists the average ratios for these genes. It is apparent from the table that the majority of selected genes were stimulated or inhibited at least 2 fold.  
       EXAMPLE 7  
       [0031]     RT-PCR and Real-Time RT-PCR  
         [0032]     Total RNA (10 μg) was treated with DNAse (Invitrogen), purified by Rneasy MinElute Cleanup kit (Quigen, Valencia, Calif.) and reverse transcribed by Superscript First-Strand Synthesis System (Invitrogen). The resulting cDNA was diluted 100 times in water and aliquots were subjected to quantitative real-time PCR in an iCycler (BioRad). The PCR mixture (25 μl) contained 5 μl of the diluted cDNA, 2.5 μl of each primer (0.04 μM) and 12.5 μl of SYBR Green (Applied Biosystems, Warrington, UK). Each sample was amplified in triplicate. Reactions were performed with initial hot start incubation at 95° C. for 10 min, followed by 40 cycles of denaturation at 95° C. for 10 sec and 60° C. for 1 min. The mixture was then incubated at 60° C. for 10 min, heated gradually in 0.4 increments and the melting curve was then analyzed. Relative gene expression was recalculated by ΔΔCt method. Briefly, the difference (ΔCt) between the Ct values of the target and the house-keeping gene (GAPDH or β-actin) were calculated: 
 
Δ Ct=Ct (target)− Ct (GAPDH) 
 
 ΔΔCt is the difference between UV-irradiated and control samples: 
 
ΔΔ Ct=ΔCt (UV)−Δ Ct (control) 
 
 The fold-time stimulation of gene expression is calculated as =2 ΔΔCt  
 
         [0033]     Primers used for the PCR were as follows: prostaglandin-endoperoxide synthase 2 (COX-2) P898 (ATGAGTGTGGGATTTGACCAG, forward, SEQ ID No. 1) and P899 (TTCCGGTGTTGAGCAGTTTTC, reverse, SEQ ID No. 2); TNRSF9 P900 (AGCTACAAAGAGGACACGAAG, forward, SEQ ID No. 3) and P901 (CGCAGCTCTAGTTGAAAGAAG, reverse, SEQ ID No. 4); Keratinocyte growth factor receptor P904 (GATGGTGCGGAAGATTTTGTC, forward, SEQ ID No. 5) and P905 (GCCGCTTTTCCATCTTTTCTG, reverse, SEQ ID No. 6); stromelysin 2 P908 (ATTTTGGCCCTCTCTTCCATC, forward, SEQ ID No. 7) and P909 (TGATGGCCCAGAACTCATTTC, reverse, SEQ ID No. 8); stromelysin 1 P910 (GGAAATCAATTCTGGGCCATC, forward, SEQ ID No. 9) and P911 (CATCGATTTTCCTCACGGTTG, reverse, SEQ ID No. 10); aquaporin 3 P912 (TTTGGCTTTGCTGTCACTCTG, forward, SEQ ID No. 11) and P913 (AAGCACATGGCAAAGGTCAC, reverse, SEQ ID No. 12); aquaporin 9 P916 (TTTGCTGGTGGAAAACTGCTG, forward, SEQ ID No. 13) and P917 (AAATGCGTTCGCCAGAGATAG, reverse, SEQ ID No. 14); keratin 1 K1-L (TGACCAAGGTGGACCTTCAG, forward, SEQ ID No. 15) and K1-R (ATGATGCTGTCCAGGTCGAG, reverse, SEQ ID No. 16); involucrin IVL-L (TCAGCCTTACTGTGAGTCTGG, forward, SEQ ID No. 17) and IVL-R (AGTCTTGAGGAGCTCCTGAC, reverse, SEQ ID No. 18); GAPDH G-L (TGATGACATCAAGAAGGTGGTGAAG, forward, SEQ ID No. 19) and G-R (TCCTTGGAGGCCATGTAGGCCAT, reverse, SEQ ID No. 20); β-actin BA-L (GCCCTGGCACCCAGCACAAT, forward, SEQ ID No. 21) and BA-R (TCATAGTCCGCCTAGAAGCA, reverse, SEQ ID No. 22).  
         [0034]     The 283 bp long band corresponding to human Gastrin was amplified by primers P432 (TGCAGACGAGATGCAGCGAC, SEQ ID No. 23) and P433 (GTCCATCCATCCATAGGCTTC, SEQ ID No. 24). The reaction mixture (25 μl) contained 2.0 mM MgCl 2 , 0.25 of each dNTP, 0.4 μM of each primer, 75 mM Tris-HCl (pH 8.8), 20 mM (NH 4 )2SO 4 , 0.01% Tween 20 and 1.25 u of Taq polymerase (Promega). The mixture was heated to 94° C. for 2.5 minutes and then amplified for 25 cycles: 94° C. for 10 seconds (denaturation), 55° C. for 10 seconds (annealing) and 72° C. for 30 seconds (extension). Amplification products were separated by agarose electrophoresis and visualized by ethidium bromide staining. The identified PCR products were excised from the agarose gel and purified using a GFX PCR DNA and gel band purification kit (Amersham-Pharmacia-Biotech). Sequencing was performed at the Molecular Resource Center at the University of Tennessee HSC (Memphis) using Applied Biosystems 3100 Genetic Analyzer and BigDye™ Terminator Kit.  
       EXAMPLE 8  
       [0000]     Statistical Analysis  
         [0035]     Data was analyzed using one-way analysis of variance and appropriate post-hoc test or by Student t test using Prism 4.00 software (GraphPad Software, San Diego, Calif.).  
       EXAMPLE 9  
       [0000]     Technological Considerations  
         [0036]     Exposure to sunlight is the leading cause of skin cancer due to its UV component (Ichihashi et al., 2003). A number of genes have been reported to be involved in the response to UVR including those coding for the cytokines IL-1β, IL-6, IL-10 (Kimbauer et al., 1991; Grewe et al., 1995). These data, generated by conventional approach were, however, concentrated on a limited number of well-known genes and consequently limited in scope. To overcome such constraints, DNA microarrays searching for a range of genes that might be differentially expressed in human keratinocytes in response to UV irradiation were developed. The known limitation of this technology was the small number of experimental samples that could be tested simultaneously versus the large number of variables (probes); thus statistically significant effects could reflect experimental biasing while lack thereof might reflect low sensitivity, rather than absence of biological effect. To minimize possible bias, detected effects were verified by RT-PCR.  
         [0037]     Another important aspect of microarray technology is data normalization; for which there are several approaches (Bilban et al., 2002). The most widely accepted method was used herein: LOWES normalization (intensity dependent). The strongest factors affecting data reliability were the technical quality of the slides and intrinsic precision and consistency of the individual techniques. Sometimes it might be impossible to eliminate false positives due to nonspecific dye binding, but this could be solved by dye-flip experiments, as presented in  FIG. 1 . Briefly, if the dot is green on the first slide it should be red on the second with the opposite dye combination. This was exemplified in  FIG. 1  where both dye combinations showed identical changes of gene expression (up- or down-regulation).  
         [0038]     DNA microarray with 761 probes was specifically designed herein to investigate neuroendocrine functions in the skin, including probes for the barrier function (Slominski and Wortsman, 2000). Genes were chosen to reflect functional pathways activated in response to environmental stressors or pathological events; e.g. carbohydrate metabolism-related hormones; pituitary and thyroid hormones; steroidogenic enzyme receptors. Genes coding for neurotransmitters, catecholamines, growth factors, cytokines, housekeeping genes and others involved in the protective function of the skin were also added. The full list of the genes as well as oligonucleotide sequences are listed on Table 1.  
         [0039]     The oligonucleotide design approach used herein was a modification of the commonly used technique of searching for oligos with desired melting temperature, preferably at the 3′ end of the gene. Instead the oligos were placed on the borders between different exons to avoid unspecific labeling of DNA impurities. It is well known that human introns contain a wide range of simple repetitive sequences of which polyA sequences are most common. Hence, the use of total RNA and poly(dT) sequence to synthesize cDNA for microarray experiments may result in some unspecific labeling since poly(dT) sequence may hybridize to introns. This concept was tested by hybridizing cDNA synthesized from total and polyA RNA of HaCaT cell line (data not shown). The polyA RNA sample showed lower signal but, when normalized the conditions showed identical results.  
                                             TABLE 1                           DNA Microarray Chips for Neuroendocrine Functions in the Skin                Short   Full                       Primer   gene   gene   Accession       name   name   name   number   Primer sequence   Comments               M001   GCG   Glucagon   NM_002054   TTCAAGACACAGAGGAGAAATC             50 bp; e2-3                           AGATCATTCTCAGCTTCCCAGG                                 GAC                       (SEQ ID NO: 25)               M002   GCGR   glucagon   NM_000160   TGGTACCTGCCTTGGCACCACAA             50 bp; e4-5               receptor       GTGCAACACCGCTTCGTGTTCAA                                 AG                       (SEQ ID NO: 26)               M003   CRH-R1           GTGCCAGGAGATCCTCAATGAG             50 bp; e4-5                       AGAAAAAAAGCAAGGTGCACTA                                 CATG                       (SEQ ID NO: 27)               M004               AGGAGATCCTCAATGAGGAGAA             40 bp; e4-5                       AAAAGCAAGGTGCACTA                       (SEQ ID NO: 28)               M005               ATCCTCAATGAGGAGAAAAAAA             30 bp; e4-5                       CAAGGTG                       (SEQ ID NO: 29)               M006               GTGCCAGGAGATCCTCAATGAG             50 bp, 62% GC,                       AGGAGCCTGGGACTCAGGCACA             e4-cryptic;                       CCTG   detects CRF-R1h                       (SEQ ID NO: 30)               M007               AGGAGATCCTCAATGAGGAGGA             40 bp, 62% GC                       CCTGGGACTCAGGCACA   e4-cryptic;                       (SEQ ID NO: 31)   detects CRF-R1h               M008               ATCCTCAATGAGGAGGAGCCTG             30 bp, 62% GC                        GACTCAG   e4-cryptic;                       (SEQ ID NO: 32)   detects CRF-R1h               M009               GTTCTACTGTTTCCTCAATAGTG             50 bp; e13-14;                       GGTCCGTTCTGCCATCCGGAAGA                                 GT                       (SEQ ID NO: 33)               M010               ACTGTTTCCTCAATAGTGAGGT             40 bp; e13-14;                       GTTCTGCCATCCGGAA                       (SEQ ID NO: 34)               M011               CAACTCCTTCCTGGAATCCTTCC             50 bp; e13-15;                       GGTCCGTTCTGCCATCCGGAAGA             detects deletion                       GT   of exon 13                       (SEQ ID NO: 35)               M012               CCTTCCTGGAATCCTTCCAGGT             40 bp; e13-15;                       GTTCTGCCATCCGGAA   detects deletion                       (SEQ ID NO: 36)   of exon 13               M013   5HTR2B   Serotonin   XM002541   ATTGCCCTCTTGACAATAATGTT             50 bp, e1-2;               receptor 2B       GAGGCTATGTGGCCCCTCCCACT             full-length                       GT                       (SEQ ID NO: 37)               M014               CCTCTTGACAATAATGTTTGAG             40 bp, e1-2;                       TATGTGGCCCCTCCCA   full-length                       (SEQ ID NO: 38)               M015               TGACAATAATGTTTGAGGCTAT             30 bp, e1-2;                        GGCCCC   full-length                       (SEQ ID NO: 39)               M016               ATTGCCCTCTTGACAATAATGTT             50 bp, e1-3;                        GGCATTGCCATTCCAGTCCCTAT             5HTR2          detects                       AA   deletion of exon 2                       (SEQ ID NO: 40)               M017               CCTCTTGACAATAATGTTTGGCA             40 bp, e1-3; 5HTR2                                 TGCCATTCCAGTCCCT   detects deletion of                       (SEQ ID NO: 41)   exon 2               M018               TGACAATAATGTTTGGCATTGC             30 bp, e1-3; 5HTR2                                 TTCCAG   detects deletion of                       (SEQ ID NO: 42)   exon 2               M019   5HTR7   Serotonin   XM015676   CCCTGTGCGTGATCAGCATTGA             50 bp, e1-2; 58% GC               receptor 7       GGTACCTTGGGATCACAAGGCC                                 TC                       (SEQ ID NO: 43)               M020               TGCGTGATCAGCATTGACAGGTA             40 bp, e1-2; 58% GC                       CTTGGGATCACAAGGC                       (SEQ ID NO: 44)               M021               GATCAGCATTGACAGGTACCTT             30 bp, e1-2; 58% GC                       GATCAC                       (SEQ ID NO: 45)               M022   CRF-R2           CACCCCTGGACCCCGAGGGTCC             40 bp; e4-5; 65% GC;           alpha           ACTCCTACTGCAACAC   detects alpha isoform                       (SEQ ID NO: 46)               M023   CRF-R2           CATGACCCTCACCAACCTCTCA             50 bp; e2-5; 65% GC;           beta           TCCCTACTCCTACTGCAACACGA             detects beta isoform                       CT                       (SEQ ID NO: 47)               M024   CRF-R2           CCCTCACCAACCTCTCAGGTCC             40 bp; e2-5; 65% GC;           beta           ACTCCTACTGCAACAC   detects beta isoform                       (SEQ ID NO: 48)               M025   CRF-R2           CTTTCCTCAGCTCTTCTGCCAAG             50 bp; e3-5; 54% GC;           gamma           TCCCTACTCCTACTGCAACACGA             detects gamma isoform                       CT                       (SEQ ID NO: 49)               M026   CRF   Corticotropin       CGAGCAGTTAGCACAGCAAGCT             50 bp               releasing       ACAGCAACAGGAAACTCATGGA                         factor       ATTA                       (SEQ ID NO: 50)               M027   Uro   Urocortin       CTTCTCTGTCCATTGACCTCACC             50 bp                       TCACCTGCTGCGGACCCTGCTG                                 G                       (SEQ ID NO: 51)               M028   CRFBP   Corticotropin       ATAAAGACAGACCCCAACCTCT             50 bp; est E1-2               releasing       CCTTGCAATGTCATTTCTCAGA                         factor       CC               binding       (SEQ ID NO: 52)               protein               M029   FSH   Follicle   NM_000510   TGTGCTGGCTACTGCTACACCA             49 bp, e2-3, 55% GC           beta   stimulating       GATCTGGTGTATAAGGACCCAG                         hormone,       A               p-p b                 (SEQ ID NO: 53)               M030   FSHR   Follicle   NM_000145   GAACCTTCCCAACCTTCAATAT             50 bp, e3-4, 38% GC               stimulating       GTTAATATCCAACACAGGTATTA                         hormone       GC               receptor       (SEQ ID NO: 54)               M031   CGB   chorionic   NM_000737   CACCAAGGATGGAGATGTTCCA             48 bp, e1-2, 58% GC;               gona                 GGGCTGCTGCTGTTGCTGCTGCT             It should also detect               tropin, beta       A   CGB7, CGB8, proba                         polypeptide       (SEQ ID NO: 55)   also CGB1, CGB5,                           CGB6               M032   GLYCA   glycoprotein   AH007338   CTCCATTCCGCTCCTGATGTGCA             50 bp, e2-3, 56% GC;               alpha subunit       GATTGCCCAGAATGCACGCTAC                                 GGA                       (SEQ ID NO: 56)               M033               CTGTGTAGCTAAATCATATAACA             50 bp, e3-4, 42% GC;                       GGTCACAGTAATGGGGGGTTTC                                 AAG                       (SEQ ID NO: 57)               M034   LHB   Luteinizing   NM_000894   TGTGCCGGCTACTGCCCCACCAT             47 bp, e1-2, 67% GC;               hormone beta       ATGCGCGTGCTGCAGGCGGTCC             high homology to C                         p-p       (SEQ ID NO: 58)               M035   LHCGR   Luteinizing   NM_000233   TCAATGGGACGACACTGACTTCA             50 bp, e6-7, 46% GC;               hormone       TGGAGCTAAAGGAAAACGTACA                         receptor       CTG                       (SEQ ID NO: 59)               M036   GNRH1   Gonadotropin-   NM_000825   CCTCCGAGACCTGAAAGGAGCT             50 bp, e2-3, 50% GC;               releasing       GGAAAGTCTGATTGAAGAGGAA                         hormone       CTG                       (SEQ ID NO: 60)               M037   GNRH2   Gonadotropin-   NM_001501   CCCAGAATGCCCTTAGGCCCCCA             47 bp, e2-3, 67% GC;               releasing       GAAGGGCCCTGGACACTGCAGC                         hormone       (SEQ ID NO: 61)               M038   GNRHR   gonadotropin-   NM_000406   CGGGTCCTTCATCAGGACCCCCA             50 bp, e2-3, 52% GC;               releasing       GAACTACAACTGAATCAGTCCA               hormone       GAA                       (SEQ ID NO: 62)               M039   GNRH2   gonadotropin-   NM_057163   TAGTTTCCTGCTTGCCTTCCCCC             50 bp, e2-3, 56% GC;               releasing       GCTGTTCCTGTTCCACACGGTCC                         horomone       CT               type 2       (SEQ ID NO: 63)               M040   GH2   growth       TTCCAACAGGGTGAAAACGCAG             50 bp, e3-43, 50% GC                         horomone       AGAAATCTAACCTAGAGCTGCT             more specific for GH                                 GCA   than GH1, but can                       (SEQ ID NO: 64)   detect all GH, CSH               M041   GH1   growth   NM_000515   CAGGGAGGAAACACAACAGAAA             50 bp, e3-4, 54% GC,               horomone       CCAACCTAGAGCTGCTCCGCAT             will also detect CSH                                 CCC   CSH2, GH2                       (SEQ ID NO: 65)               M042               AGCCTGGTGTACGGCGCCTCTGA             47 bp, e4, more speci                                 AGCAACGTCTATGACCTCCTAAA             for GH1 than to CSH                       (SEQ ID NO: 66)               M043   GHR   growth   NM_000163   AAGTGTTTCTCTGTTGATGAAAT             50 bp, e4-5, 44% GC;               horomone       GTGCAACCAGATCCACCCATTG                         receptor       CT                       (SEQ ID NO: 67)               M044   GHRH   growth   NM_021081   CCACCTCCCCCTTTGACCCTCAG             47 bp, e1-2, 62% GC;               horomone       ATGCGGCGGTATGCAGATGCCA                         releasing       (SEQ ID NO: 68)               horomone               M045   GHRHR   growth   NM_000823   TTCTCTCACTTCAGCTCAGAGTC             50 bp, e3-4, 48% GC;               horomone       GGGGCTGTGAAACGGGATTGTA                         releasing       TAT               horomone       (SEQ ID NO: 69)               receptor               M046   ghrelin   ghrelin   AB029434   GAGCCCTGAACACCAGAGAGTC             50 bp, e1-2, 56% GC;                       AGCAGAGAAAGGAGTCGAAGAA             homol to motilin                       CCAC   (the same?)                       (SEQ ID NO: 70)               M047   MC1R   melanocortin   XM_047456   AACCTCTTTCTCGCCCTCATCAT             50 bp, only 1 exon,               1 receptor       GCAATGCCATCATCGACCCCCT             52% GC; specific for                       T   MC1R                       (SEQ ID NO: 71)               M048   MC2R   melanocortin   NM_000529   GATCGTCCTGCTGGCTGTGTTCA             50 bp, only 1 exon,               2 receptor       GAATAAGAATCTCCAGGCACCC             50% GC; specific for                       TGT   MC2R                       (SEQ ID NO: 72)               M049   POMC   proopiomelano             NM_000939   CTCACCACGGAAAGCAACCTGC             46 bp, e2-3, 63% GC;               tin       GGAGTGCATCCGGGCCTGCAAG                                 (SEQ ID NO: 73)               M050               TCAGAGAGCAGCCTCCCGAGAC             46 bp, e1-2, 60% GC;                       GAGCCTCAGCCTGCCTGGAAGA                                 (SEQ ID NO: 74)               M051   LOC5105   preproprolactin   NM_015893   ACTCCATGGAGATCCGCACCCCT             48 bp, e1-2, 58% GC;               releasing       ACATCAATCCTGCCTGGTACGC             palindrom at 5′ end               peptide       (SEQ ID NO: 75)               M052   PrRPR   prolactin   AB048946   TGCTGCTGGTCACCTACCTGCTC             49 bp, only 1 exon,               releasing       CTCTGCTGGTCATCCTCCTGTCT             57% GC;               peptide       (SEQ ID NO: 76)               receptor               M053   PRL   prolactin   XM_033558   GGGCATGGAGCTGATAGTCAGC             50 bp, e4-5, 48% GC;                       AGGTTCATCCTGAAACCAAAGA             may detect peptides                       AATG   similar to prolactin                       (SEQ ID NO: 77)               M054   PRLR   prolactin   NM_000949   GGATTTGATGCTCATCTGTTGGA             50 bp, e9-10, 46% GC                         receptor       AAGGGCAAGTCTGAAGAACTA             should be absent whe                                 GAG   is alternatively                       (SEQ ID NO: 78)   spliced in cancer                           (PRLR1b)               M055   SST   somatostatin   NM_001048   TGGCTGCTGCCGCGGGGAAGCA             47 bp, e1-2, 62% GC;                       GAACTGGCCAAGTACTTCTTGG             have a palindron                       (SEQ ID NO: 79)               M056   SSTR1   somatostatin   NM_001049   CCTCTATGGCTTTCTCTCAGACA             50 bp, only 1 exon,               receptor 1       CTTCAAGCGCTCTTTCCAACGCA             50% GC; specific for                       CC   SSTR1                       (SEQ ID NO: 80)               M057   SSTR2   somatostatin   XM_085745   GGCTATCCATTCCATTTGACCTC             50 bp, only 1 exon,               receptor 2       ATGGCTCTGTGGTGTCAACCAA             50% GC; specific for                       CC   SSTR2                       (SEQ ID NO: 81)               M058               TCAATCAGTTCACCAGCATCTT             50 bp, only 1 exon,                       GCCTGACAGTCATGAGCATCGA             50% GC; less specific                       GA   may also detect SST                                 (SEQ ID NO: 82)   3, 5 genes               M059   SSTR3   somatostatin   NM_001051   CGCTCTTCGTGCTCTGCTGGATG             50 bp, only 1 exon,               receptor 3       CCTTCTACGTGCTCAACATCGTC             56% GC; specific for                       AC   SSTR3                       (SEQ ID NO: 83)               M060   SSTR4   somatostatin   XM_009594   GTCTTCGTGGTCTACACTTTCCT             47 bp, only 1 exon,               receptor 4       CTGGGCTTCCTGCTGCCCGTGCT             59% GC; may also                       (SEQ ID NO: 84)   detect SSTR1               M061   SSTR5   somatostatin   NM_001053   TCACCAACATCTACATTCTCAA             50 bp, only 1 exon,               receptor 5       TGGCAGTGGCCGACGTCCTGTA             59% GC; may also                       TG   detect SSTR2, 3, 4                       (SEQ ID NO: 85)               M062   TRH   thyrotropin-   XM_002795   AAGGGGACCAGGGTGAGCACT             48 bp, e2-3, 56% GC;               releasing       GCGTCCCAGATCTITTCAATCTGA                         horomone       T                       (SEQ ID NO: 86)               M063   TRHR   thyrotropin-   XM_017795   CAGCACAGTATCTTCAAGGAAG             50 bp, e1-2, 52% GC;               releasing       AGGTCACCAAGATGCTGGCAGT                         horomone       GTTG               receptor       (SEQ ID NO: 87)               M064   TSHb   Thyroid stimu-   NM_000549   CTGTGCTGGATATTGTATGACA             50 bp, e1-2, 42% GC;               lating hormon,       GGATATCAATGGCAAACTGTTT                         beta subunit       TC                       (SEQ ID NO: 88)               M065   TSHR   Thyroid stimu-   NM_000369   GCTTACCGCCCAGTACGCAGACT             50 bp, e1-2, 52% GC;               lating hormon       TGAAGCTTATTGAGACTCACCT                         receptor       GA                       (SEQ ID NO: 89)               M066   DIO1   type I 5′   S48220   TGCAACATTTGGGAGTTTATGCA             50 bp, e1-2, 42% GC;               iodothyronine       GGTAATAGGCCACTGGTGCTGA                         deiodinase       TT                       (SEQ ID NO: 90)               M067   DIO2   type 2 5′   NM_000793   CTGGAAGAGCTTCCTCCTCGAT             50 bp, e2-3, 52% GC;               iodothyronine       CTACAAACAGGTGAAATTGGGT                         deiodinase v2       AGG                       (SEQ ID NO: 91)               M068   DIO3   type 3 5′   NM_001362   GCTACCAGGTCTCTGAGCTGCG             50 bp, only 1 exon               iodothyronine       CTTGGTTGGAACGCTATGATGA             54% GC;               deiodinase       AA                       (SEQ ID NO: 92)               M069   SLC26A4   Iodine   NM_000441   AGAGCACTGGAGGAAAGACACA             50 bp, e9-10, 52% GC                         transport       GTTGCTGGCATCATCTCTGCTG                                 ATT                       (SEQ ID NO: 93)               M070   THRA   thyroid   XM_008585   ACTTGTGAGGGCTGCAAGGGCT             50 bp, e4-5, 54% GC;               horomone       TTTCGCCGCACAATCCAGAAGAA                         receptor,       CT               alpha       (SEQ ID NO: 94)               M071   THRB   thyroid   NM_000461   ATCTATGTTGGCATGGCAACAGA             50 bp, e6-7, 48% GC;               hormone       TTGGTGCTGGATGACAGCAAGA                         receptor, bet       GCT                       (SEQ ID NO: 95)               M072   TG   thyroglobulin   NM_003235   TAGGGAAGCCCAAGAAATGCCC             50 bp, e9-10 (the                       ACGCCCTGTCAAUACAGGCTGA             biggest), 54% GC;                       CAA                       (SEQ ID NO: 96)               M073               CAAGGGCGGGAACTGGCTGAGA             50 bp, e7-8 (Assuming                       AGGTTTGGAGTTGTTACTGGAT             that it can also be                       AAT   shorter protein),                       (SEQ ID NO: 97)   50% GC               M074   AG   Androgen   L29496   TATGAAGCAGGGATGACTCTGG             50 bp, e3-4, 50% GC;               receptor       AGCCCGGAAGCTGAAGAAACTT                                 GTAA                       (SEQ ID NO: 98)               M075   SBP   plasma sex   X05792   AGGGACTCAGGCAGAATTCAAT             50 bp, 50% GC; will               steroid-       TCCGAGACATTCCCCAGCCTCAT             also detect SHGB,               binding       CAG   SHGB-2               protein       (SEQ ID NO: 99)               M076   ESR1   estrogen   XM_045967   TGCTACGAAGTGGGAATGATGA             50 bp, e3-4, 48% GC;               receptor 1       AGGTGGGATACGAAAAGACCGA                                 GAGG                       (SEQ ID NO: 100)               M077   ESRRB o             estrogen   AB006590   TTACGAAGTGGGAATGGTGAAG             50 bp, e3-4, 53% GC;           ESR2   receptor       GTGGCTCCCGGAGAGAGAGATG                         beta       GGGT                       (SEQ ID NO: 101)               M078   ESRRA   estrogen-   NM_004451   ATCAGCTGGGCCAAGAGCATCC             50 bp, e3-4, 58% GC;               related       AGGCTTCTCATCGCTGTCGCTGT                         receptor       TGA               alpha       (SEQ ID NO: 102)               M079       estrogen   L25275   GACTCTGTTGGATCAGAAGGTCA             50 bp, e4-5, 50% GC;               sulfotrans-       GGTGGTCTATGTTGCCCGAAAC             may also detect phen                         ferase       AA   and aryl                       (SEQ ID NO: 103)   sulphotransferase               M080   REA   repressor of   NM_007273   GAGCAGCCCAGAATATCTCCAA             50 bp, e6-7, 50% GC               estrogen       ACGATCGCCACATCACAGAATC                         receptor       ATC               activity       (SEQ ID NO: 104)               M081       glucocorticoid   X03225   TGAAATGGGCAAAGGCAATAC             50 bp, e5-6, 46% GC;               receptor   M10901   GGTTTCAGGAACTTACACCTGGA             detects both alpha and                   X03348 M11   GAG   beta receptors                       (SEQ ID NO: 105)               M082       alpha-   X03225   GACGATGAGTATTGAATTCCCC             50 bp, e10, 42% GC;               glucocorticoid   M10901   GATGTTAGCTGAAATCATCACCA             detects only alpha               receptor       TC   receptor = NR3C1                       (SEQ ID NO: 106)               M083   LOC1432   similar to   XM_089709   ACAAAGCAGGGAAGAGAAACTT             50 bp, e2-3, 42% GC               glucocorticoid       AGGTGGGAGCATGTGTCAGTCTT                         receptor       CCC                       (SEQ ID NO: 107)               M084   MR   mineralocorticoid   M16801   CTTCAAGCTGGAATGAATTTAG             50 bp, e4-5, 40% GC;               receptor       GCACGAAAGTCAAAGAAGTTG             detects full-length =                       AAA   NR3C2                       (SEQ ID NO: 108)               M085       mineralocorticoid   AJ315514   CTTCAAGCTGGAATGAATTTAG             50 bp, e4-?, 44% GC;               receptor delta       GAGAGAAGATGCATCAGTCTGC             should detect delta                       ATG   isoform                       (SEQ ID NO: 109)               M086   BZRP   benzodiazapine   NM_000714   TTCTTTGGTGCCCGACAAATGG             50 bp, e2-3, 58% GC;               receptor       TGGGCCTTGGTGGATCTCCTGCT                                 GT                       (SEQ ID NO: 110)               M087   NR1H2   nuclear   NM_007121   CCTCCTGAAGGCATCCACTATC             50 bp, e7-8, 56% GC;               receptor       GATCATGCTGCTAGAGACAGCC                         subfamily 1,       GGC               group H       (SEQ ID NO: 111)               member 2               M088   NR1I3   nuclear   NM_005122   GTGAGGGCTGCAAGGGTTTCTT             50 bp, e3-4, 56% GC;               receptor       GGAGAACAGTCAGCAAAAGCAT                         subfamily 1,       GGT               group I,       (SEQ ID NO: 112)               member               M089   NR2C1   nuclear   NM_003297   TGATCTGTCTGCACAACACCTG             50 bp, e2-3, 48% GC;               receptor       GCTCCTAACAGATAATTCTCCA                         subfamily 2,       CC               group C       (SEQ ID NO: 113)               member 1               M090   NR2C2   nuclear   NM_003298   TTCAGACACACACGTCACATTTA             50 bp, e10-11 46% G                         receptor       GCTAACAATGCCCAGTCCAATG             may detect some               subfamily 2,       AG   orphan receptors               group C,       (SEQ ID NO: 114)               member 2               M091   NR2F2   nuclear   NM_021005   AAGGCCATAGTCCTGTTCACCT             50 bp, e2-3, 52% GC;               receptor       GATGCCTGTGGTCTCTCTGATGT                         subfamily 2,       GC               group F,       (SEQ ID NO: 115)               member 2               M092   NR4A1   nuclear   NM_002135   TGGCGGTGGGCATGGTGAAGGA             47 bp, e3-4, 57% GC;               receptor       GTTGTCCGAACAGACAGCCTGA                         subfamily 4,       G               group A,       (SEQ ID NO: 116)               member 1               M093   NR0B1   nuclear   NM_000475   CGCTTCTGTACCGCTGCTGCTTT             44 bp, e1, 60% GC;               receptor       GCGGTGAAGACCACCCGCAGCA                         subfamily 0,       (SEQ ID NO: 117)               group B,               member 1               M094   NR2F1   nuclear   NM_005654   TCAAAGTGGGCATGAGGCGGGA             47 bp, e1-2, 57% GC;               receptor       GCGGTTCAGCGAGGAAGAATG             has homology to orphan               subfamily 2,       T   receptor (COUP-TFI)               group F,       (SEQ ID NO: 118)               member 1               M095   NR1H4   nuclear   NM_005123   GCACTGACCTGTGAGGGGTGTA             50 bp, e4-5, 48% GC               receptor       AGGTTTCTTCAGGAGAAGCATTA             farnesol receptor               subfamily 1,       CAA               group H,       (SEQ ID NO: 119)               member 4               M096   NR2E1   nuclear   AF411525   TGAGCAAGCCAGCCGGATCAAC             50 bp, e1-2, 50% GC               receptor       AGCCGCATTTTAGATATCCCCTG                         subfamily 2       AAA               group E       (SEQ ID NO: 120)               member 1               M097   NR4A2   nuclear   NM_006186   TGTITTGTCCTTCGATTAGCATAC             50 bp, e6-7, 48% GC;               receptor       GGTCCAACCCAGTGGAGGGTAA             almost the same as               subfamily 4,       CTC   NGFI-B/nur77 (acc               group A,       (SEQ ID NO: 121)   S77154)-has several               member 2           exons deleted               M098   NR4A3   nuclear   NM_006981   CTCAGTGTTGGAATGGTAAAAG             50 bp, e6-7, 42% GC               receptor       AGTTGTCCGTACAGATAGTCTGA             neuron derived orphan               subfamily 4,       AGG   receptor (NOR-1,               group A,       (SEQ ID NO: 122)   #D78579)               member 3               M099   NR5A1   nuclear   NM_004959   TGCTCACGTGTGAGAGCTGCAA             56 bp, e1-2, 58% GC =               receptor       GGCTTCTTCAAGCGCACGGTGCA             steroidogenic factor               subfamily 5,       (SEQ ID NO: 123)   1(#U76388)               group A,               member 1               M100               AAGTTCATCATCCTCTTCAGCCT             50 bp, e5-6, 40% GC                       GATTTGAAGTTCCTGAATAACCA                                 AT                       (SEQ ID NO: 124)               M101   NR6A1   nuclear   NM_033334   CGCCCTCCGATGAAGAACTACA             50 bp, e5-6, 50% GC =               receptor       AGATTTAGTGATGAAGGGATGG             gem cell nuclear               subfamily 6,       GGTG   receptor, GCNF               group A,       (SEQ ID NO: 125)   (S83309)               member 1               M102   PGR   progesterone   NM_000926   CTTCTTTAAGAGGGCAATGGAA             50 bp, e2-3, 44% GC               receptor       GGCAGCACAACTACTTATGTGCT                                 GAA                       (SEQ ID NO: 126)               M103       Delta       GCTGTCAGGCTGGCATGGTCCTT             50 bp, e2-3, 46% GC                   isoform       GAGGTTTTCGAAACTTACATATT                                 AT                       (SEQ ID NO: 127)               M104   PGRMC1   progesterone   NM_006667   AGTGACTGGGAGTCTCAGTTCA                         receptor       TTCAAGTATCATCACGTGGGCAA                         membrane       CT               component 1       (SEQ ID NO: 128)               M105   PGRMC2   progesterone   NM_006320   CGAGAATGGGAAATGCAGTTTA             50 bp, e2-3, 36% GC               receptor       AGAAAAATATGATTATGTAGGC                         membrane       GACT               component 2       (SEQ ID NO: 129)               M106   RARA   retinoic acid   NM_000964   TTTGAAGTGGGCATGTCCAAGGA             50 bp, e4-5, 46% GC               receptor,       GTCTGTGAGAAACGACCGAAAC                         alpha       AGAA                       (SEQ ID NO: 130)               M107   RARB v1   retinoic acid   NM_000965   GGTCAGCGCCTGTGAGGGATGT             50 bp, e2-3, 52% GC;           (NR1B2)   receptor,       AGGGCTTTTTCCGCAGAAGTATT             detects both v1 and               beta, v                 AGA   v          isoforms                       (SEQ ID NO: 131)               M108   RARG   retinoic acid   NM_00096   ACCTCCGGGGCATCAGCACTAA             49 bp, e6-7, 55% GC               receptor,       GGAGCTGAAAGGGCCATTACTC                         gamma       GAA                       (SEQ ID NO: 132)               M109   RXRA   retinoid X   NM_002957   ATCTGCGGGGACCGCTCCTCAG             46 bp, e3-4, 58% GC               receptor,       AAGCACTATGGAGTGTACAGCT                         alpha       (SEQ ID NO: 133)               M110   RXRB   retinoid X   NM_021976   CAATCTGCGGGGACAGAAGCTC             48 bp, e1-2, 50% GC               receptor,       GGCAAACACTACGGGGTTTACA                         beta       CT                       (SEQ ID NO: 134)               M111   AKR1D1   aldo-keto   NM_005989   TGGTACCTACTCAGAACCTAAAT             50 bp, e1-2, 52% GC               reductase       GACCCCTAAGGGAGCCTGTGCA                         family 1       CAT               member D1       (SEQ ID NO: 135)               M112   CYP11A   cytochrome P45             NM_000781   TCCAGAAGTATGGCCCGATTTA             48 bp, e1-2, 54% GC               subfamily X1A       GGGAGAAGCTCGGCAACGTGGA             P450 scc                       T                       (SEQ ID NO: 136)               M113   CYP11B1   cytochrome P4             NM_000497   AAATGTGGCGTGTTCTTGCTGAA             50 bp, e2-3, 50% GC               subfamily X1B       GGGCCTGAATGGCGCTTCAACC             (steroid 11-beta-               polypeptide 1       ATT   hydroxylase) will also                       (SEQ ID NO: 137)   detect CYP11B2               M114   CYP17   cytochrome P4             NM_000102   ACTTCTCTGGGCGGCCTCAAAT             48 bp, e2-3, 54% GC               subfamily XVI                 CAACTCTAGACATCGCGTCCAA             Steroid 17-alpha-                       (SEQ ID NO: 138)   hydroxylase               M115   CYP19   cytochrome P4             NM_000103   GCTCTTCTTGAGGATCCCTTTGG             50 bp, e5-6, 46% GC               subfamily XIX       CGAAAGTGCTATCGTGGTTAAA             aromatization of                       CC   androgens                       (SEQ ID NO: 139)               M116   CYP21A2   cytochrome P4             NM_000500   TGTGGACGTGATTCCCTTTCTCA             50 bp, e5-6, 54% GC               subfamily XXI                 GTTCTTCCCCAATCCAGGTCTCC             Steroid 21-hydroxylase                       GA                       (SEQ ID NO: 140)               M117   CYP24   cytochrome P4             XM_030593   CTCAAGAAACAGCACGACACCC             50 bp, e1-2, 50% GC               subfamily XXI                 GGTGGAGTACCACAAGAAGTAT             vitamin D 24-                       GCAA   hydroxylase                       (SEQ ID NO: 141)               M118   CYP27A1             cytochrome P4             NM_000784   GGTTGGAATGCCATCTTTTCCTT             50 bp, e4-5, 44% GC               subfamily       GGGAAGAAGCTGATTGATGAGA             Steroid 27-hydroxylase               XXVIIA       GCT                       (SEQ ID NO: 142)               M119   CYP2C9   cytochrome P4             NM_000771   CTGCTGAAGCACCCAGAGGTCA             48 bp, e6-7, 50% GC               subfamily IIC       AGCTAAAGTCCAGGAAGAGATT             Mephenytoin 4-                       AA   hydroxylase; will also                       (SEQ ID NO: 143)   detect CYP2C8, 17,           19               M120   CYP3A4   cytochrome P4             NM_017460   AGTCGCCTCGAAGATACACAAA             50 bp, e6-7, 48% GC               subfamily IIIA       GCACCGAGTGGATTTCCTTCAG             niphedipine oxidase                       GAT   will detect other CYP                                 (SEQ ID NO: 144)   genes               M121   HSD17B1   17beta-   AF037438   AGTGTGGCTGCCTTCGAGGGTCA             = ERAB (U96132)               hydroxysteroid       GTTGGACAAGCTGCATACTCTG                         dehydrogenase       T               type 10       (SEQ ID NO: 145)               M122   HSD17B3   hydroxysteroid   NM_000197   CCTGAACGCACCGGATGAAATC             50 bp, e5-6, 50% GC               (17-beta)       AGAGCCTCATCCATTGTAACAT                         dehydrogenase       CCT                       (SEQ ID NO: 146)               M123   HSD17B8   hydroxysteroid   NM_014234   AACATCAGTAGCATCGTAGGAA             50 bp, e4-5, 51% GC               (17-beta)       GGTGGGGAACGTGGGGCAGACA                         dehydrogenase       ACT                       (SEQ ID NO: 147)               M124       adrenaline   E07358   TTCGTGCTCTGCTGGTITCCCCTT             50 bp, 50% GC               alpha2CII       TCTTCAGCTACAGCCTGTACGG                         receptor       T                       (SEQ ID NO: 148)               M125       adrenaline   E14946   AGGCTTTGCCAACGGCTCGACG             50 bp, e1-2, 57% GC               beta receptor       GGCTTCTTGGGGAGTTTCTTAG                                 (SEQ ID NO: 149)               M126   PNMT   phenylethanola             NM_002686   AACATGCCTGCCTCATTGAGGG             46 bp, e2-3, 54% GC               ne N-methyl       AGGGGGAATGCTGGCAGGATAA                         transferase       (SEQ ID NO: 150)               M127   SLC6A2   solute carrier   NM_001043   TTCTACCGCTTGAAAGAGGCCA             50 bp, e5-6, 50% GC               family 6       GTATGGATTGATGCCGCAACTCA             neurotransmitter                       AT   transporter,                       (SEQ ID NO: 151)   noradrenalin               M128   ADM   adrenomedullin   NM_001124   TGTCGCGTCGGAGTTTCGAAAGA             50 bp, e2-3, 52% GC                       GTGGAATAAGTGGGCTCTGAGT                                 GTG                       (SEQ ID NO: 152)               M129   ADMR   adrenomedullin   NM_007264   TCCCAAGGGAAACTCAGGCGTG             46 bp, e1-2, 55% GC               receptor       GCTGGTCCCAATGTCAGTGAAA                                 (SEQ ID NO: 153)               M130   ANGPT1   angiopoietin 1   NM_001146   CAACCTTGTCAATCTTTGCACTA             50 bp, e4-5, 38% GC                       AGAAGGTGTTTTACTAAAGGGA                                 GAA                       (SEQ IDNO: 154)               M131   ANGPT2   angiopoietin 2   XM_034835   ATCCCAGTCCACCTGAGGA   50 bp, e9, 46% GC                       ACTGTCTCGAACTATTTTCAAA                                 CTTAAGC                       (SEQ ID NO: 155)               M132   ANGPT4   angiopoietin 4   NM_015985   AAGAAAGGGCTAACGCCTCGG             47 bp, e4-5, 59% GC;                       CCGGCCTTCATAATGGCAGGTGA             angiopoetin-3                       (SEQ ID NO: 156)               M133   CDT6   angiopoietin-   NM_021146   AGACGGTCACTCAGACCTCCGC             47 bp, e1-2, 57% GC               like fact       GATGCCATCTACGACTGCTCTT                                 (SEQ ID NO: 157)               M134   LOC1643   similar to   XM_092738   AACTGGATCTGAGCGCAAAAAG             50 bp, only 1 exon               Angiopoietin 1       AAGACACAGACGGTCAAAAAC             50% GC               receptor       CCCC               precursor       (SEQ ID NO: 158)               M135   TEK   TEK tyrosine   XM_005480   TGTGGAGTCAGCTTGCTCCTTTC             50 bp, e1-2, 50% GC               kinase       GGAACTGTGGAAGGTGCCATGG                                 CTT                       (SEQ ID NO: 159)               M136   BDKRB1   bradykinin   NM_000710   CTGCTGCACAGAGTGCTGCCAA             50 bp, e2, 52% GC               receptor B1       TTTATCATCTCCATCTGTTTCTT                                 G                       (SEQ ID NO: 160)               M137   BDKRB2   bradykinin   XM_040854   TCCGTGCCCACCACGGCCTCTTT             46 bp, e1-2, 60% GC               receptor B2       AGCGCCGACATGCTCAATGTCA                                 (SEQ ID NO: 161)               M138   PTGIS   prostaglandin   NM_000961   TGATGAAAAGGCCAGGATGAAA             50 bp, e3-4, 50% GC               I2 (prosta-       TGACTCTTCTCCACAGAGAGCT                         cyclin)       AGG               synthase       (SEQ ID NO: 162)               M139   CT   Calcitonin   X00356 J001   TCATGTTAGCATGCCCCAGAAT             50 bp, e3, 48% GC                       CAACTAAACTCCTCCCTTTTCCTT                                 CT                       (SEQ ID NO: 163)               M140   CALCA   Calcitonin   M64486   ATCATTGCCCAGAAGAGAGCCT             49 bp, e3, 54% G               with different       GACACTGCCACCTGTGTGACTCA             Detected in medullar               C-term   C   thyroid carcinoma                       (SEQ ID NO: 164)               M141   CALCB   calcitonin-   XM_113645   TCAAGCCTGAGCAGATGAATGA             50 bp, e8-9, 50% G               related       TCCAGGAAGAAGGTTATCATGA                         polypeptide,       ACTG               beta       (SEQ ID NO: 165)               M142   CALCR   calcitonin   NM_001742   AGGGCCGTGTACTTCAATGACA                         receptor       TGCTGGCTGAGTGTGGAAACCCA                                 TT                       (SEQ ID NO: 166)               M143   ECE2   endothelin   NM_014693   AGCCACTGAGAGACCTCATTGA             49 bp, e5-6, 49% GC               converting       AAGATTGGTGGTTGGAACATTA                         enzyme 2       GG                       (SEQ ID NO: 167)               M144   ECE2   Variants A   AF428263   GGCTGCCTTGTGGCCCTAGGGGT             47 bp, e3-9, 55% GC                   AF428264   TATGGGATGGATTATTFGCAGCA                                 (SEQ ID NO: 168)               M145   ECE1   endothelin   NM_001397   CTCTCATCAACACCACCGACAG             50 bp, e9-10, 50% G               converting       GCCTGCTCAACAACTACATGAT                         enzyme 1       GG                       (SEQ ID NO: 169)               M146   EDN1   endotbelin 1   XM_165812   ACATCATTTGGGTCAACACTCCT             50 bp, e2-3, 50% GC                       AGCACGTTGTTCCGTATGGACTT                                 GA                       (SEQ ID NO: 170)               M147   EDN2   endothelin 2   NM_001956   ACATCATCTGGGTGAACACTCCT             50 bp, e2-3, 54% GC                       AACAGACAGCTCCTTACGGCCT                                 GA                       (SEQ ID NO: 171)               M148   EDN3   endothelin 3   XM_030725   ACATCATTTGGATCAACACTCC             49 bp, e2-3, 53% GC                       AACAGACGGTGCCCTATGGACT                                 C                       (SEQ ID NO: 172)               M149   EDNRA   endothelin   NM_001957   TGATCTCCCTATCAATGTATTTA             50 bp, e2-3, 50% GC               receptor       GCTGCTGGCTGGGCGCTGGCCTT                         type A       TG                       (SEQ ID NO: 173)               M150   EDNRB   endothelin   XM_007108   TGACATCCCTATCAATGTCTACA             50 bp, e1-2, 50% GC               receptor       GCTGCTGGCAGAGGACTGGCCA             very similar to M149               type B       TG                       (SEQ ID NO: 174)               M151               GTCTATGTGCTCTGAGTATTGA             50 bp, e2-3, 50% GC                       GATATCGAGCTGTTGCTTCTTGG             sure only EDNRB                       GT                       (SEQ ID NO: 175)               M152   CCK   cholecystokinin   NM_000729   AGATACATCCAGCAGGCCCGGA             50 bp, e2-3, 54% GC                       AGCTCCTTCTGGACGAATGTCCA                                 CGT                       (SEQ ID NO: 176)               M153   CCKBR   cholecystokinin   NM_000731   TGCAAGGCGGTTTCCTACCTCAT             47 bp, e2-3, 57% GC;               receptor       GGGGTGTCTGTGAGTGTGTCCA             will also detect gastric                       (SEQ ID NO: 177)   receptor               M154   GAS   gastrin   NM_000805   CCCACACCTCGTGGCAGACCCGT             41 bp, e1-2, 63% GC                       CAAGAAGCAGGGACCAT                       (SEQ ID NO: 178)               M155               CGACTATGTGTGTATGTGCTGAT             49 bp, e1, 53% GC                       TTTGCACTGGCTCTGGCCGCCTT                                 T                       (SEQ ID NO: 179)               M156   GRP   gastrin-   NM_002091   AAAGATGTAGGTTCAAAAGGCA             50 bp, e2-3, 44% GC               releasing       AGTTGGTAGACTCTCTGCTCCA                         peptide       TTC                       (SEQ ID NO: 180)               M157   GRPR   gastrin-   NM_005314   AGGGAATATACATGTCAAGAAG             50 bp, e1-2, 46% GC               releasing       AGATTGAATCCCGGAAGCGACT                         peptide       GCCA               receptor       (SEQ ID NO: 181)               M158   LEP   leptin   NM_000230   CAGGATCAATGACATTTCACACA             50 bp, e1-2, 46% GC                       GCAGTCAGTCTCCTCCAAACAGA                                 AG                       (SEQ ID NO: 182)               M159   LEPR   Leptin   NM_002303   ACCTTTTCATGGCCTATGAGCAA             50 bp, e15-16, 46% G                                 GTAAATATCGTGCAGTCACTCA                                 GC                       (SEQ ID NO: 183)               M160   MCH-R2   melanin-   AF399937   TCATGACTGTAATGAGTGTGGA             50 bp, e2-3, 46% GC               concentrating       GGTACTTTGCCCTCGTCCAACCA                         hormone       TT               receptor       (SEQ ID NO: 184)               M161   PMCH   pro-melanin-   XM_006590   CGAAGAGGAAAATAAAGTTTCA             50 bp, e1-2, 34% GC               concentrating       AGAACACAGGCTCCAAACATAA                         hormone       TTCT                       (SEQ ID NO: 185)               M162               CATGCTCAGATGTATGCTGGGAA             50 bp, e3, 50% GC                       AGTCTACCGACCTTGTTGGCAA                                 CT                       (SEQ ID NO: 186)               M163       Something       GTAGCGTTAAGATAGATCAGTA             50 bp, 36% GC               sim          from       CCAAAATCATAGGCTTTTTCTGT                         chromosome 5       CAT                       (SEQ ID NO: 187)               M164   CABP1   calcium   NM_031205   GGAACTGCGAGATGCTTTCCGA             50 bp, e5-6, 50% GC               binding       AGTTTGACACCAATGGTGATGG                         protein 1 v1       GAAA                       (SEQ ID NO: 188)               M165       Variant 2       TCCACTGAGAAATCTCTCAAGGA             50 bp, e1-3, 46% GC                       GGATAGATCACTGCGACCAGAG                                 AAA                       (SEQ ID NO: 189)               M166   CABP2   calcium   NM_016366   GCAGCATAAAGGACTCAAGGA             47 bp, e7, 55% GC               protein 2 v1       ACAGCCTCTGCCCACCAGCATGT                                 (SEQ ID NO: 190)               M167   CABP3   calcium   NM_016367   CTCAAGAAATGCTTCCCGCAGCT             50 bp, e1-2, 50% GC               binding       TTCAATCTCATCTTGTCCCAGTG                         protein 3       TC                       (SEQ ID NO: 191)               M168   CABP5   calcium   NM_019855   CACTGGGACAAGATGAGATTGA             50 bp, e1-2, 48% GC               binding       GAGCTGCGGGAAGCATTTCTTGA                         protein 5       TTC                       (SEQ ID NO: 192)               M169   CALM1   calmodulin 1   NM_006888   AACTGTCATGAGGTCACTGGGT             50 bp, e2, 50% GC                       GAACCCAACAGAAGCTGAATTG             will also detect                       AGG   calmodulin 2                       (SEQ ID NO: 193)               M170   CALM2   calmodulin 2?   D45887   AGAAGCATTCCGTGTGTTTGATA             50 bp, e3-4, 48% GC                       GGATGGCAATGGCTATATTAGT                                 TG                       (SEQ ID NO: 194)               M171   CALM3   calmodulin 3   NM_005184   ATATGATCAATGAGGTGGATGC             50 bp, e3-4, 48% GC                       GATGACCTTCCAGGGAACGGGA                                 CATTGA                       (SEQ ID NO: 195)               M172   CALN1   calneuron 1   NM_031468   CACGATAGACAGCATATTCTGG             50 bp, e3-4, 44% GC                       GTTTGACATGCAAAGGATAACT                                 GG                       (SEQ ID NO: 196)               M173   CAMKK2   calcium/calmo             XM006592   CACGTCTCCATCACGGGTATGCA             49 bp, e2-3, 51% GC               n-dependent       GACTGTGTGCAGCTGAATCAGTA                         protein kinase       A               kinase 2       (SEQ ID NO: 197)               M174   PDE1A   phosphodiester             NM_005019   GAAACAGTTTGCAGCAGCCTGA             50 bp, e10-11, 50% G                         1A, calmodulin       GGGATTGACAGAGCCAAAACCA                         dependent       GTCC                       (SEQ ID NO: 198)               M175   MLN   motilin   NM_002418   CTATGGCGAACTCCAGAGGATG             50 bp, e2-3, 50% GC                       AGGAAAAGGAACGGAATAAAG                                 CAAA                       (SEQ ID NO: 199)               M176   NPPB   natriuretic   NM_002521   ACTTGGAAACGTCCGGGTTACA             46 bp, e2, 56% GC;                peptide       GAGCAGCGCAACCATTTGCAGG                         precursor B       (SEQ ID NO: 200)               M177   NPPC   natriuretic   NM_024409   CAAATACAAAGGAGCCAACAA             48 bp, e1-2, 52% GC               peptide       AGGGCTTGTCCAAGGGCTGCTT                         precursor C       (SEQ ID NO: 201)               M178   NPR1   natriuretic   NM_000906   CGGATGTGGAACCGAAGCTTTC             50 bp, e3-4, 52% GC               peptide       AGGTGTGACAGGATACCTGAAA                         receptor A       TTGA                       (SEQ ID NO: 202)               M179               GGATCCCGAGAATGGTGCCTTC             50 bp, e5-6, 50% GC                       GGTTGTACTGAACTACAATGGG                                 TT                       (SEQ ID NO: 203)               M180   NPR2   natriuretic   NM_003995   AAAGATGCAGGGACGAAGATAT             50 bp, e4-5, 50% GC               peptide       ACGGTGTAACTGGGCTGGTTGT                         receptor B       TGG                       (SEQ ID NO: 204)               M181               TTGGACGACCCATCCTGTGATAA             50 bp, e6-7, 46% GC                       ACTCCACTTTCAACCCTGGCAAT                                 GT                       (SEQ ID NO: 205)               M182   NPR3   natriuretic   NM_000   CAATATCCAGGCCAGTGAGAGA             48 bp, e1-2, 52% GC               peptide       TGGTGATCATGTGTGCGAGCAG                         receptor C       A                       (SEQ ID NO: 206)               M183   KIAA097   netrin G1f   NM_014917   CCCTCCTGAGACGTTCTGTGCAA             50 bp, e2-3, 50% GC                       GGGCAATCCCTACATGTGCAATA                                 TG                       (SEQ ID NO: 207)               M184   KIAA185   netrin G2   NM_032536   CCCTGAGAGGTTCTGCTCCCAT             48 bp, e1-2, 54% GC                       GAATCCCTACCTATGCAGCAAC                                 (SEQ ID NO: 208)               M185   NTN1   Netrin 1   NM_004822   CAGCAGCGTGGAGGAGCCTGAA             45 bp, e3-4, 59% GC                       ACTGCGATTCCTACTGCAAGG                       (SEQ ID NO: 209)               M186   NTN4   Netrin 4   NM_02122   TTCTCAGCTCCAGATGCTTGCAA             49 bp, e1-2, 51% GC                       CCGTGTTCCTGCCATCCAGTAG                                 T                       (SEQ ID NO: 210)               M187   iNOS   nitric oxide   AF051164   CAGGATGACCCTAAGAGTCACA             50 bp, 48% GC               synthase       GCATCAAAATGGTTTCCCCCAGT             will also detect NOS                                 CCT                       (SEQ ID NO: 211)               M188   NOS1   nitric oxide   NM_000620   CAACTCTGTGCAGGAAGAAAGG             50 bp, e15-16, 50% G                         synthase 1       AGAGCTACAAGGTCCGATTCAA                                 AGCG                       (SEQ ID NO: 212)               M189   NOS2   nitric oxide       TGACACAGGATGACCTTCAGTA             50 bp, 48% GC in one               synthase 2       ACAACCTCAGCAAGCAGCAGAA             exon will also detect                       GAG   iNOS                       (SEQ ID NO: 213)               M190   NOS3   nitric oxide       CACAGGAAATGTTCACCTACAT             50 bp, 46% GC               synthase 3       GCAACCACATCAAGTATGCCAC                                 AAC                       (SEQ ID NO: 214)               M191   PEDF   pigment   M76979   GCCTCCCGGATCGTCTTTGAGA         A   50 bp, e4-5, 50% GC               epithelium-       AAGCTGCGCATAAAATCCAGCT                         differenti-       GT               ation factor       (SEQ ID NO: 215)               M192   NPY5R   neuropeptide   NM_006174   GCTACTGTCTGGACACTAGGTTT             50 bp, single exon,               Y          receptor Y5       GCCATCTGTTCTCCCCTTCCAGT             50% GC                       TT                       (SEQ ID NO: 216)               M193       neuropeptide   U42389   AATGGGTCCAATAGGTGCAGAG             50 bp, single exon,               y/peptide YY       CTGATGAGAACCAGACAGTGGA             50% GC               receptor type 2       GAAA                       (SEQ ID NO: 217)               M194   PPY2   pancreatic   NM_021092   TTGGAGCCATTGTACCGAGGGG             44 bp, 54% GC               polypeptide 2       AATACCACACCGGAGCAGAT                       (SEQ ID NO: 218)               M195   PPYR1   pancreatic   NM_005972   TACACCATCATGGACTACTGGAT             50 bp, single exon,               polypeptide       TTTGGAGAGACCCTCTGCAAGAT             48% GC               receptor 1       TC                       (SEQ ID NO: 219)               M196   PYY   peptide YY   NM_004160   ACTACCTCAACCTGGTCACCCG             41 bp, e2-3, 54% GC                       AGCGGTATGGGAAAAGA                       (SEQ ID NO: 220)               M197   PYY2   peptide YY, 2   NM_021093   TACGCCTATCCTCGCCAGTACCT             46 bp, single exon,                       ATCCTGGTCACTCAGCCGTCGT   54% GC                       (SEQ ID NO: 221)               M198   VIP   vasoactive   NM_003381   TTACAGGGCACCTTCTGCTCT             50 bp, e1-2, 50% GC               intestinal       GGTTGGGTGACAGAATACCCTTT                         peptide       AG                       (SEQ ID NO: 222)               M199               AGTCTCTTATGGGAAAACGTGTT             50 bp, e3-4, 42% GC                       GCAGTAACATCTCAGAAGACCC                                 GTA                       (SEQ ID NO: 223)               M200   PTH   parathyroid   NM_000315   AAATCGGATGGGAAATCTGTTA             50 bp, e2-3, 38% GC               hormon       GAAGAGATCTGTGAGTGAAATA                                 AGCT                       (SEQ ID NO: 224)               M201   PTHR1   parathyroid   NM_000316   GGTGCCCATCCTGGCCTCCATT             50 bp, e10-11, 50% G                         hormone       GCTCAACTTTCATGCTCTTCATCA                         receptor 1       TA                       (SEQ ID NO: 225)               M202               CATATACTGTTTCTGCAATGGC             50 bp, e14-15, 40% G                                 GGTACAAGCTGAGATCAAGAA                                 CTT                       (SEQ ID NO: 226)               M203   PTHR2   parathyroid   NM_005048   GGCATGACACAAGGAAGCAATA             50 bp, e10-11, 50% G                         hormone       AGGAAACTGGCCAAATCGACA                         receptor 2       GGTC                       (SEQ ID NO: 227)               M204               GGCAACTTCTGTGGACAAATCA             50 bp, e6-7, 44% GC                       ATATATCGGGTGCAAGATTGCT                                 TG                       (SEQ ID NO: 228)               M205   CYP4F8   cytochrome P4             NM_007253   GTCCGATCTGTCATCAATACCT             50 bp, e2-3, 44% GC               subfamily IVF,       GATGCCATTACAGACAAGGACA                         polypeptide 8       AGT                       (SEQ ID NO: 229)               M206   PTGDR   prostaglandin   XM_051711   CACTATGTGTTCTCTGCCCGTAA             50 bp, e1-2, 42% GC               D          receptor       TTATCGCGCTTACTATGGAGCAT                                 TA                       (SEQ ID NO: 230)               M207   CYP4F11   cytochrome P4             NM_021187   GCCTATCACCAGTGCCTCAGCT             47 bp, e1-2, 53% GC               subfamily IVF,       TGTCGCACCCAAGGATATGATTT                         polypeptide 11       (SEQ ID NO: 231)               M208   CYP4F12   cytochrome P4             NM_023944   AACAAGAGTGCAAACATCATGC             50 bp, e4-5, 50% GC               isoform 4F12       TGACAAGTGGCAGCACCTGGCC                                 AGA                       (SEQ ID NO: 232)               M209   CYP4F2   cytochrome P4   NM_001082   ATCCGGTCTGTCATCAACGCCT             50 bp, e3-4, 52% GC               subfamily IVF,       GCTGCCATTGCACCAAAGGACA                         polypeptide 2       GTT                       (SEQ ID NO: 233)               M210   CYP4F3   cytochrome P4   XM_029072   CATTGATGTACTCCTGCTGAGCA             50 bp, e6-7, 48% GC               subfamily IVF,       GGATGAAGATGGGAAGAAGTT                         polypeptide 3       CCG                       (SEQ ID NO: 234)               M211   PTGER1   prostaglandin E   NM_000955   CGTGCATCTGCTGGAGCCCAAT             40 bp, e2-3, 62% GC               receptor 1       TGGTGTTGGTGGCGCT                       (SEQ ID NO: 235)               M212   PTGER2   prostaglandin E   NM_000956   CGCCGTCTGCTCCTTGCCTTTCA             50 bp, e1-2, 44% GC               receptor 2       GATTTTTGCATATATGAATGAAA                                 CT                       (SEQ ID NO: 236)               M213   PTGER3   prostaglandin E   NM_000957   AGGACTTGGTGCAGTTCTCATGA             50 bp, e1-2, 48% GC               receptor 3       AGAGAACCCTGCAGTGTCCAGC                                 AAG                       (SEQ ID NO: 237)               M214   PTGER4   prostaglandin E   NM_000958   TCATCTGCTCCATCCCGCTCGTG             48 bp, e2-3, 52% GC               receptor 4       TGCGAGTATTCGTCAACCAGTTA                                 (SEQ ID NO: 238)               M215   PTGFR   prostaglandin F   NM_000959   TCCTGTATTTGTTGGAGCCCATT             50 bp, e2-3, 42% GC               receptor       CTGGTTACAATGGCCAACATTG                                 AT                       (SEQ ID NO: 239)               M216   PTGIR   prostaglandin   NM_000960   TGTGCTCCCTGCCTCTCACGATC             37 bp, e2-3, 62% GC               I2 (prosta-       GCTGCTTCACCCA               cyclin)       (SEQ ID NO: 240)               receptor               M217   PTGS2   prostaglandin-   NM_000963   TTCTATGGAGAAAACTGCTCAA             50 bp, e2-3, 32% GC               endoperoxide       CCGGAATTTTTGACAAGAATAA                         synthase 2       ATT                       (SEQ ID NO: 241)               M218               CAGACAAGCAGGCTAATACTGA             50 bp, e7-8, 38% GC                       AGGAGAGACTATTAAGATTGTG                                 TTGA                       (SEQ ID NO: 242)               M219   TBXA2R   thromboxane A   NM_001060   TCTCTGTCGCTTCATGGGCGTCG             44 bp, 54% GC               receptor       CATGATCTTCTTCGGCCTGT                       (SEQ ID NO: 243)               M220   TBXAS1   thromboxane A   NM_001061   TTTCAGTCCTGAAAAGCTGAAC             50 bp, e4-6, 33% GC               synthase 1       GATGGTTCCCCTCATCAGCCAA                                 CT                       (SEQ ID NO: 244)               M221               GGTAGACGTTTTTAAGGAGAAA             49 bp, e9-10, 51% GC                       ACATGGCCCCTGAGTTCTGCAG                                 TC                       (SEQ ID NO: 245)               M222   AGTR1   angiotensin   XM_051470   TGTACGCTAGTGTGTTTCTACTC             50 bp, 46% GC               receptor 1       CGTGTCTCAGCATTGATCGATA                                 TG                       (SEQ ID NO: 246)               M223   AGTR2   angiotensin   XM_030897   ATTTACTCCTTTTGGCTACTCTT             50 bp, 36% GC               receptor 2       TCTATGGGCAACCTATTATTCTT                                 T                       (SEQ ID NO: 247)               M224   ACE   angiotensin I   XM_044372   CTTCTACAACAGGAAAGACTTCA             50 bp, e6-7, 50% GC               converting       GATCAAGCAGTGCACACGGGTC                         enzyme       CGA                       (SEQ ID NO: 248)               M225   AGT   angiotensinoge   NM_000029   TTCAACACCTACGTCCACTTCCA             50 bp, e2-3, 52% GC                       GGGAAGATGAAGGGCTTCTCCC                                 GCT                       (SEQ ID NO: 249)               M226   REN   renin   NM_000537   CGACAGACACCACCACCTTTAAA             50 bp, e1-2, 50% GC                       GGATCTTCCTCAAGAGAATGCC                                 CA                       (SEQ ID NO: 250)               M227               GGAGCCAAGAAGAGGCTGTTTG             49 bp, e7-8, 51% GC                       TTATGTCGTGAAGTGTAACGAG                                 CC                       (SEQ ID NO: 251)               M228   RENBP   renin binding   NM_002910   AAGTATGTGTGGCTGCAGGGGA             49 bp, e3-4, 53% GC               protein       GCAGGTATGGATGTATTGTCGC                                 GT                       (SEQ ID NO: 252)               M229   ACE2   ACE-related   AF291820   AGAACTGAAGTTGAAAAGGCCA             50 bp, e16-17, 48% G                         carboxypeptidase       CAGGATGTCCCGGAGCCGTATC                                 ATGA                       (SEQ ID NO: 253)               M230   SCT   secretin   NM_021920   CTGGTGGGGAAGCGCAGCGAG             47 bp, e2-3, 65% GC                       GGACGCAGAGAACA                                 (SEQ ID NO: 254)               M231   SCTR   Secretin   NM_002980   GGATGCTCACCAGCAGAAATGG             49 bp, e3-4, 51% GC               receptor       TCCTTGTTCCGAAACTGCACACA                                 GA                       (SEQ ID NO: 255)               M232   UTS2   Urotensin 2   NM_021995   ATGCTGGGTGCAGAAAGAGGG             50 bp, 48% GC                       TATTCTCAGGAAAGCAGACTCA                                 GTAC                       (SEQ ID NO: 256)               M233   AQP4   aquaporin 4   NM_001650   CAGTTATCATGGGAAATTGGGA             50 bp, e3-4, 44% GC                       AACCATTGGATATATTGGGTTG                                 CCC                       (SEQ ID NO: 257)               M234   AVPR1A   arginine   XM_006934   ACAGAACATTGTCTTACTTGAT             50 bp, e1-2, 42% GC               vasopressin       TCCCGATGACCTCAACAACAGG                         receptor 1A       AAG                       (SEQ ID NO: 258)               M235               AGATTTCAAGTCCAGCATCTCAA             50 bp, e2-3, 44% GC                       GCGACAACCTTGGTAACTCTGCA                                 GA                       (SEQ ID NO: 259)               M236   AVPR1B   arginine   NM_000707   CGGTGACCATGCTCACGGCCTG             41 bp, e2-3, 61% GC               vasopressin       ACAGCCTCATCTGCCAT               receptor 1B       (SEQ ID NO: 260)               M237               CCACCAATGTGGCTTTCACCAT             50 bp, e3, 50% GC                       CTAGCTTTTGGGCAACCTCAA                                 GC                       (SEQ ID NO: 261)               M238   AVPR2   arginine   NM_000054   ATGCTCATGGCGTCCACCACTT             40 bp, e1-2, 62% GC               vasopressin       GCTGTGCCTGGGCATC               receptor 2       (SEQ ID NO: 262)               M239   OXTR   oxytocin   NM_000916   ATGCCAACGCGCCCAAGGAAG             43 bp, e1-2, 58% GC               receptor       TCGGCCTTCATCATCGTCAT                       (SEQ ID NO: 263)               M240   OXT   oxytocin   NM_000915   AAAGGCCGCTGCTTCGGGCCCA             39 bp, e2, 61% GC                       ATCTGCTGCGCGGAA                       (SEQ ID NO: 264)               M241       oxytocinase   U62769   CACCGCTTTATCAAATATGCCTA             50 bp, e3-4, 40% GC                       GAAGTCATCAGTCGTTCTAGAT                                 TG                       (SEQ ID NO: 265)               M242   RLN1   relaxin 1   NM_006911   CCTCAGACACCTAGACCAGTGG             50 bp, e1-2, 46% GC                       AGAAATTGTACCATCCTTCATCA             will also detect                       CAA   relaxin 2                       (SEQ ID NO: 266)               M243               CTATCTGAGAGGCAACCATCATT             50 bp, e2, 48% GC                       CCAGAGCTACAGCAGTATGTAC             specific for                       GC   relaxin 1                       (SEQ ID NO: 267)               M244   RLN2   relaxin 2   NM_006911   GAGAGTTCCTTGGTGCCCTTTC             50 bp, e2-3, 46% GC                       AATTGTGCCATCCTTCATCAACA                                 AG                       (SEQ ID NO: 268)               M245   RLN3   relaxin 3   NM_080864   ATCCTGGCCCACGAGGCTATGG             45 bp, e1-2, 57% GC                       AGATACCTTCCCGGATGCAGAT                                 (SEQ ID NO: 269)               M246       vasopressin   AF031476   ATGTCCGACCTGGAGCTGAGAC             36 bp, e1-2, 63% GC               precursor       GTGCCTCCCCTGC                       (SEQ ID NO: 270)               M247               TTTGCTGCAACGACGAGAGCTG             34 bp, e2-3, 61% GC                       TGACCGAGCC                       (SEQ ID NO: 271)               M248   CHRNA1   cholinergic   NM_000079   GACAACCAATGTGCGTCTGAAA             50 bp, e3-4, 42% GC               receptor,       AGCAATGGGTGGATTACAACCT                         nicotinic,       AAAT               alpha poly-       (SEQ ID NO: 272)               peptide 1               (muscle)               M249               TATTCTACCTGCCCACAGACTCA             50 bp, e6-7, 42% GC                       GGGAGAAGATGACTCTGAGCAT                                 TCT                       (SEQ ID NO: 273)               M250   CHRNA2   cholinergic   NM_000742   ACCACCAACGTCTGGCTAAAAC             49 bp, e4-5, 53% GC               receptor,       GGAGTGGAGCGACTACAAACTG                         nicotinic,       GCT               alpha poly-       (SEQ ID NO: 274)               peptide 2               (neuronal)               M251   CHRNA4   cholinergic   NM_000743   TCATGGAGACCAACCTGTGGCT             50 bp, e3-4, 48% GC               receptor,       AGCAAATCTGGAATGACTACAA                         nicotinic,       CTG               alpha poly-       (SEQ ID NO: 275)               peptide 3               M252   CHRNA4   cholinergic   NM_000744   GTCCATCGCTCAGCTCATTGAC             50 bp, e2-3, 50% GC               receptor,       GGATGAGAAGAACCAGATGAT                         nicotinic,       CCA               alpha poly-       (SEQ ID NO: 276)               peptide 4               M253   CHRNA5   cholinergic   XM_007577   CACCAGACATCGTTTTGTTTGAT             50 bp, e4-5, 44% GC               receptor,       ATGCAGATGGACGTTTTGAAGG                         nicotinic,       ACC               alpha poly-       (SEQ ID NO: 277)               peptide 5               M254   CHRNA6   cholinergic   CHRNA6   GGAAACCAATTTGTGGCTGCGT             50 bp, e2-3, 42% GC               receptor,       CATCTGGAATGATTATAAATTG                         nicotinic,       CT               alpha poly-       (SEQ ID NO: 278)               peptide 6               M255   CHRNA7   cholinergic   NM_000746   GAGCCTCCTGCAGATCATGGAC             50 bp, e1-2, 48% GC               receptor,       GGATGAGAAGAACCAAGTTTTA                         nicotinic,       CCA               alpha poly-       (SEQ ID NO: 279)               peptide 7               M256   CHRNB1   cholinergic   NM_000747   ATCCTGGCGCAACTCATCAGCCT             49 bp, e2-3, 51% GC               receptor,       AACGAGAAGGATGAAGAGATGA                         nicotinic,       CA               beta poly-       (SEQ ID NO: 280)               peptide 1               (muscle)               M257   CHRNB2   cholinergic   NM_000748   AGCAGATCATGACCACCAATGT             50 bp, e3-4, 50% GC               receptor,       GGCTGACCCAGGAGTGGGAAGA                         nicotinic,       TAT               beta poly-       (SEQ ID NO: 281)               peptide 2               (neuronal)               M258   CHRNB3   cholinergic   NM_000749   TTCCTGACATAGTTCTCTTTGAA             50 bp, e1-2, 48% GC               receptor,       ATGCTGACGGCCGCTTCGAAGG                         nicotinic,       CC               beta poly-       (SEQ ID NO: 282)               peptide 3               M259   CHRNB4   Cholinergic   XM_039151   AGAATGACGATGAAGACCAGA             50 bp, e1-2, 50% GC               receptor,       GTCGTTGAGGACTGGAAGTACG                         nicotinic,       GGCT               beta poly-       (SEQ ID NO: 283)               peptide 4               M260   DRD1   dopamine   XM_003966   AGACTTTGCCCTGCGACGAATAA             50 bp, 44% GC               receptor       GCCATAGAGACGGTGAGTATCA                         D1       TAA                       (SEQ ID NO: 284)               M261   DRD2   dopamine   NM_000795   CTCCTCTTCGGACTCAATAACG             50 bp, e5-6, 50% GC               receptor       GACCAGAACGAGTGCATCATTG                         D2       CAA                       (SEQ ID NO: 285)               M262   DRD3   dopamine   XM_113416   TTCTGTTTGGCTTTAATACCACA             49 bp, e4-5, 51% GC               receptor       GGGACCCCACTGTCTGCTCCAT                         D3       C                       (SEQ ID NO: 286)               M263   DRD4   dopamine   XM_006145   TGGTGCTGCCGCTCTTCGTCTA             36 bp, e1-2, 63% GC               receptor       CCGAGGTCCAGG               D4       (SEQ ID NO: 287)               M264   AANAT   arylalkylamine   NM_001088   CTTTGAGATCGAGCGTGAAGCC             44 bp, e2-3, 54% GC               acetyl-       CATCTCCGTCTTGGGCGTCT               transferase       (SEQ ID NO: 288)               M265               ACAAGGAGAGACTCATGCAGGA             39 bp, e2-3, 53% GC                       TCACTGACGCTGCACA                                 (SEQ ID NO: 289)               M266   TPH   Tryptophan   X52836   GATCTGAACTAGATGCAGACCA             50 bp, e4-5, 46% GC               hydroxylase       CTGGCTTCAAAGACAATGTCTA                                 GT                       (SEQ ID NO: 290)               M267               ATAAAAGCCCTGAAAATCTTTCA             49 bp, e1-3, 46% GC                       GTATGGAAACTGTTCCTTGGTTT             might detect deletion                       C   exon 2 (regulatory                       (SEQ ID NO: 291)   domain)               M268               CAGATCCCTTCTATACCCCAGA             50 bp, e6-7, 50% GC                       CAGATACCTGCCATGAACTCTTA                                 GT                       (SEQ ID NO: 292)               M269   HIOMT   acetylserotonin   NM_004043   AAGAACAGATTGACTTCCAGGA             50 bp, e7-8, 46% GC               methyl-       GGGGATTTCTTCAAAGACCCTCT                         transferase       CCG                       (SEQ ID NO: 293)               M270   ABAT   4-aminobutyrat             NM_000663   GGAAGTCCCAGGGCCTAGATCT             50 bp, e2-3, 44% GC               amino-       AGGAGTTAATGAAACAGCTGAA                         transferase       ATAA                       (SEQ ID NO: 294)               M271   AOX1   aldehyde   NM_001159   GCCAGAATTTGAGGAAGGAAGT             50 bp, e6-7, 44% GC               oxidase 1       AGACAAGTCCAAAACTCTTCGC                                 GAAG                       (SEQ ID NO: 295)               M272   DBH   dopamine   NM_000787   TACCACAACCCACTGGTGATAGA             48 bp, e5-6, 52% GC               beta-       AGGACGAAACGACTCCTCAGG                         hydroxylase       TC                       (SEQ ID NO: 296)               M273   CHAT vN   choline   NM_020549   CGTCATCGTAGCCTGCTGCAAT             50 bp, e6-7, 44% GC               acetyl-       GTTCTTTGTCTTGGATGTTGTCA             detects all variants               transferase       A                       (SEQ ID NO: 297)               M274       Variant N1       TGAGGCTTTGAGAAAGGAGTAG             50 bp, e1, 50% GC                       AGCCTAGCATTCCGGCAGAGGA                                 GAAA                       (SEQ ID NO: 298)               M275   COMT   catechol-O-   NM_000754   AGAGGGCTGGAGCCTGCTCAGA             42 bp, e1-2, 59% GC               methyl-       GTGCTTTGAAGATGCCGGA   will detect only MB               transferase       (SEQ ID NO: 299)   isoform               M276               CATGAACGTGGGCGACAAGAAA             46 bp, e3-4, 54% GC                       GCAAGATCGTGGACGCCGTGAT             will detect all                       (SEQ ID NO: 300)   isoform               M277   DDC   dopa   XM_033775   AAAGGACTGCAGGCTTATATCC             49 bp, e9-10, 51% GC                         decarboxylase       AAGCATGTCCAGCTGTCCCATGA                                 T                       (SEQ ID NO: 301)               M278   GAD2   glutamate   NM_000818   CTCTGCTCTCCTGGTTAGAGAA             50 bp, e12-13, 48% G                         decarboxylase       GGGATTGATGCAGAATTGCAAC                         2       AAA                       (SEQ ID NO: 302)               M279   GAD2   glutamate   NM_013445   GACTTCTCTAATCTGTTTGCTAG             50 bp, e3-4, 46% GC               decarboxylase       GATCTGCTTCCGGCTAAGAACG             Detects both 25 and                       GA   isoforms                       (SEQ ID NO: 303)               M280       glutamine   Y00387   GACAGTGAGCCCAAGTGTGTGG             50 bp, e3-4, 52% GC;               synthetase       AGAGTTGCCTGAGTGGAATTTC             should detect also so                                 TGG   gene on chr 11                       (SEQ ID NO: 304)               M281               CATGCGGGAGGAGAATGGTCTG             50 bp, e7-8, 52% GC;                       AGTACATCGAGGAGGCCATTGA             should detect also so                                 AAAC   genes on chr 2, 9                       (SEQ ID NO: 305)               M282   HNMT   histamine   NM_006895   AAGATGCTCATTATTGTTGTGT             50 bp, e5-6, 48% GC               N-methyl       GGAAGCAGTGGCTGGGACAAG                         transferase       GTG                       (SEQ ID NO: 306)               M283   MAOA   monoamine   NM_000240   CTGGGTACAAGAACCTGAATCA             50 bp, e13-14, 50% G                         oxidase A       AGGACGTTCCAGCGGTAGAAAT                                 ACCC                       (SEQ ID NO: 307)               M284   MAOB   monoamine   NM_000898   GGGAGGCAGGACTTACACTCTT             50 bp, e2-3, 46% GC               oxidase B       GGAACCAAAAGGTTAAATATGT                                 GACC                       (SEQ ID NO: 308)               M285   HRH1   histamine   XM_052382   CCGAGAGGACAAGTGTGAGACA             50 bp, 50% GC               receptor H1       ACTTCTATGATGTCACCTGGTTC                                 AGG                       (SEQ ID NO: 309)               M286   HRH2   histamine   NM_022304   AAGGGCAATCATACCACCTCTA             50 bp, 44% GC               receptor H2       GTGCAAAGTCCAGGTCAATGAA                                 TGTA                       (SEQ ID NO: 310)               M287   HRH3   histamine   AF321910   TCAACCTCGCCATCTCCGACTT             43 bp, e1-2, 60% GC               receptor H3       TCGTCGGCGCCTTCTGCAT                       (SEQ ID NO: 311)               M288   HRH4   histamine   NM_021624   GGCCATCTCTGACTTCTTTGTGG             50 bp, e1-2, 48% GC               receptor H4       TGTGATCTCCATTCCTTTGTACA                                 C                       (SEQ ID NO: 312)               M289   MTNR1A   melatonin   NM_005958   GTATCGGAACAAGAAGCTCAGG             50 bp, e1-2, 48% GC           receptor 1A       ACGCAGGAAACATCTTTGTGGT                                 GCT                       (SEQ ID NO: 313)               M290   MTNR1B   melatonin   NM_005959   AGGAACCGCAAGCTCCGGAACG             48 bp, e1-2, 52% GC                   receptor 1B       AGGTAATTTGTTCTTGGTGAGT                                 G                       (SEQ ID NO: 314)               M291   NTS   neurotensin   NM_006183   TTCAGCTCCTGGAGTCTGTGCT             50 bp, e1-2, 44% GC                       GATTCAGAAGAGGAAATGAAA                                 ATT                       (SEQ ID NO: 315)               M292   NTSR1   neurotensin   NM_002531   CATCTCGGATGAGCAGTGGACT             50 bp, e2-3, 50% GC               receptor 1       GTTCCTCTATGACTTCTACCACT                         (high       CT               affinity)       (SEQ ID NO: 316)               M293   NTSR2   neurotensin   NM_012344   ACCGCGCTCCAAGTCTTTATCCA             47 bp, e1-2, 53% GC               receptor 2       GTGAATGTGCTGGTGTCCTTCGT                                 (SEQ ID NO: 317)               M294   ADRA1A   adrenergic,   NM_033303   TTTTCTTAGTCATGCCCATTGGG             50 bp, e1-2, 44% GC               alpha       CTTTCTTCCCTGATTTCAAGCCC                         1A-, receptor       T                       (SEQ ID NO: 318)               M295   ADRA1B   adrenergic,   NM_000679   TACCCTTCTTCATCGCTCTACCG             48 bp, e1-2,               alpha       TGGCTCCTTGTTCTCCACCCTGA             54% GC = adral c?               1B-, receptor       (SEQ ID NO: 319)               M296   ADRA1D   adrenergic,   NM_000678   TGCTGGTTCCCTTTCTTCTTTGT             45 bp, e1-2, 55% GC               alpha       TGCCGCTCGGCTCCTTGTTC               1D-, receptor       (SEQ ID NO: 320)               M297   ADRA2A   adrenergic,   NM_000681   TCTCCCTACTCTCTCCCGCCGCT             50 bp, 5′UTR, 44% G                         alpha       GAAATAAAACTTGGCTGTATTA                         2A-, receptor       A                       (SEQ ID NO: 321)               M298   ADRA2B   adrenergic,   NM_000682   TTCCTCATTCTCTTTACCATCTT             50 bp, 50% GC               alpha       GCAACGCTCTGGTCATCCTGGCT                         2B-, receptor       T                       (SEQ ID NO: 322)               M299   ADRA2C   adrenergic,   NM_000683   CAACAGCTCGCTCAACCCGGTCA             50 bp, 50% GC; may               alpha       CTACACGGTCTTCAACCAGGATT             also detect ADRA 2A               2C-, receptor       (SEQ ID NO: 323)   and 2B               c               M300   ADRB1   adrenergic,   NM_000684   TAAGACCGATAGCAGGTGAACT             50 bp, 3′UTR, 48% G                         beta       GAAGCCCACAATCCTCGTCTGA                         1-, receptor       CAT                       (SEQ ID NO: 324)               M301   ADRB2   adrenergic,   NM_000024   GAGACCTGCTGTGACTTCTTCA             49 bp, 51% GC               beta       AACCAAGCCTATGCCATTGCCT                         2-, receptor       T                       (SEQ ID NO: 325)               M302   ADRB3   adrenergic,   NM_000025   CCTGAAGGACAAGAAGCAACAA             50 bp, 3′UTR, 46% G                         beta       TCTGTTGATCAGAACCTGTGGAA                         3-, receptor       ACC                       (SEQ ID NO: 326)               M303   HTR1A   serotonin   XM_003692   AATTGGCTGGGCTACTCCAACT             50 bp, 48% GC               receptor       CTGCTTAACCCCGTCATTTACG                         1A       TA                       (SEQ ID NO: 327)               M304   HTR1B   serotonin   XM_004117   TGGAAAAGAAGAAACTCATGG             49 bp, 51% GC               receptor       GCTAGGGAGCGCAAAGCCACCA             similarity to 1 D?               1B       (SEQ ID NO: 328)               M305   HTR1D   serotonin   XM_001542   CCAAATCTTGTGTGACATCTGG             49 bp, 51% GC               receptor       GTCCTCTGACATCACGTGCTGCA                         1D       A                       (SEQ ID NO: 329)               M306   HTR1E   serotonin   XM_004134   GGAAACATGAACATCACAAACT             50 bp, 46% GC               receptor       TACCACAGAGGCCAGCATGGCT                         1E       TAAG                       (SEQ ID NO: 330)               M307   HTR1F   serotonin   XM_003092   ATTGTGTATATTGTGAGAGAGA             50 bp, 44% GC               receptor       TGGATTATGGGGCAAGTGGTCT                         1F       GA                       (SEQ ID NO: 331)               M308   HTR2A   serotonin   XM_007123   CATGTTAACCATCCTGTATGGGT             45 bp, e1-255% GC               receptor       CCGGTGGCCTCTGCCGAGCAA                         2A       (SEQ ID NO: 332)               M309   HTR2C   serotonin   XM_013121   CTGTCTCTCCTGGCAATCCTTTA             50 bp, e2-3, 44% GC               receptor       GATTATGTCTGGCCACTACCTA                         2C       TA                       (SEQ ID NO: 333)               M310   HTR3A   serotonin   XM_006444   TCCCGGACATTCTCATCAATGA             50 bp, e4-5, 48% GC               receptor       TCGTGGATGTGGGGAAGTCTCCA                         3A       AT                       (SEQ ID NO: 334)               M311   HTR3B   serotonin   XM_006445   ACCTTCAAGAGCATTCTGCATA             50 bp, e5-6, 48% GC               receptor       GTGGAAGACGTAGACCTGGCCT                         3B       CT                       (SEQ ID NO: 335)               M312   HTR4   serotonin   NM_000870   TTCCTGTAATGGACAAACTTGAT             50 bp, e5-6, 42% GC               receptor       CTAATGTGAGTTCTGAGGAGGG                         4       TC                       (SEQ ID NO: 336)               M313   HTR5A   serotonin   XM_017055   ACCCATATCCGAAGCTGTGGAG             44 bp, e5-6, 53% GC               receptor       GAAGGACTCTGCCAAACAG               5A       (SEQ ID NO: 337)               M314   HTR6   serotonin   XM_001435   TTTGTGGCCAACATAGTCCAGG             40 bp, e2-3, 55% GC               receptor       GTGTGCGACTGCATCT               6       (SEQ ID NO: 338)               M315   EGF   epidermal   NM_001963   AGAGAGATGGGAAAACATGTA             50 bp, e7-8, 48% GC               growth       GGTTGTTCCTCACCCGATAATG                         factor       GGA                       (SEQ ID NO: 339)               M316   EGFR   epidermal   NM_005228   AAGTGCGAAGGGCCTTGCCGCA             50 bp, e7-8, 48% GC               growth       AGTGTGTAACGGAATAGGTATT                         factor       GTGA               receptor       (SEQ ID NO: 340)               M317               ATACGCGGCAGGACCAAGCAA             49 bp, e10-11, 51% G                                 TGGTCAGTTTTCTCTTGCAGTC                                 CA                       (SEQ ID NO: 341)               M318   ERBB2   v-erb-b2   NM_004448   TGAACTGGTGTATGCAGATTGC             49 bp, e20-21, 51% G                         erytbroblastic       AGGGGATGAGCTACCTGGAGGA                         leukemia viral       GT               oncogene       (SEQ ID NO: 342)               homolog 2               M319   FGF1   fibroblast   NM_000800   CACCGACGGGCTTTTATACGGCT             50 bp, e3-4, 48% GC               growth       ACAGACACCAAATGAGGAATGT                         factor 1       TGT                       (SEQ ID NO: 343)               M320   FGF2   fibroblast   NM_002006   GTCCGGGAGAAGAGCGACCCT             50 bp, e1-2, 52% GC               growth       CATCAAGCTACAACTTCAAGCA                         factor 2       AAGA                       (SEQ ID NO: 344)               M321   FGF3   fibroblast   NM_005247   TGGCCATGAACAAGAGGGGAC             46 bp, e1-2, 54% GC               growth       CTCTATGCTTCGGAGCACTACA                         factor 3       (SEQ ID NO: 345)               M322   FGF4   fibroblast   NM_002007   GGCAAGCTCTATGGCTCGCCCTT             45 bp, e2-3, 57% GC               growth       TTCACCGATGAGTGCACGTTC               factor 4       (SEQ ID NO: 346)               M323   FGFS   fibroblast   NM_004464   GATCCCACGAAGCCAATATGTTA             50 bp, e1-2, 38% GC               growth       GTGTTTTGGAAATATTTGCTGTG                         factor 5       CT                       (SEQ ID NO: 347)               M324   FGF6   fibroblast   NM_020996   CAGTAAAGGAAGATTGTACGCA             50 bp, e2-3, 46% GC               growth       CGCCCAGCTTCCAAGAAGAATG                         factor 6       AAGT                       (SEQ ID NO: 348)               M325   FGF7   fibroblast   NM_002009   ACCCAAGAGATGAAGAATAATT             50 bp, e1-2, 38% GC               growth       CAATATCATGGAAATCAGGACA                         factor 7       TGGC                       (SEQ ID NO: 349)               M326   FGF8   fibroblast   NM_033163   AGAAGGGGAAGCTGATCGCCAA             45 bp, e1-2, 55% GC               growth       AGCAACGGCAAAGGCAAGGACT             will detect longer and               factor 8       (SEQ ID NO: 350)   shorter isoforms               M327   FGF10   fibroblast   NM_004465   AGAAGGGGAAACTCTATGGCTC             50 bp, e2-3, 38% GC               growth       AAAGAATTTAACAATGACTGTA                         factor 10       GCTG                       (SEQ ID NO: 351)               M328   FGF11   fibroblast   NM_004112   ATGCTGAGGGACTGCTCTACAG             48 bp, e,3-4, 54% GC               growth       CGCCGCATTTCACAGCTGAGTGT             homol to fgf-3?               factor 11       (SEQ ID NO: 352)               M329   FGF12   fibroblast   NM_021032   ACCAAGGACGAAAACAGCGACT             50 bp, e2-3, 48% GC               growth       CACTCTCTTCAATCTAATTCCCG                         factor 12       GGG                       (SEQ ID NO: 353)               M330   FGF13   fibroblast   NM_004114   AAGAAGAGGCGCAGAAGAAGA             50 bp, e1-2, 48% GC               growth       AGAGCCTCAGCTTAAGGGTATA                         factor 13       TAC                       (SEQ ID NO: 354)               M331   FGF14   fibroblast   NM_004115   ACCAAGGATGACAGCACTAATT             50 bp, e2-3, 48% GC               growth       ACACTCTTCAACCTCATACCAGT                         factor 14       GG                       (SEQ ID NO: 355)               M332   FGF16   fibroblast   NM_003868   ACCTAGGAATGAATGAGCGAG             50 bp, 48% GC               growth       GAACTCTATGGGTCGAAGAAAC                         factor 16       CACA                       (SEQ ID NO: 356)               M333   FGF17   fibroblast   NM_003867   TTCTCTGCTGTCAAACTCAGGG             50 bp, e2-3, 48% GC               growth       AGAATCACCCGTCTCCTAATTTT                         factor 17       AC                       (SEQ ID NO: 357)               M334   FGF18   fibroblast   NM_033649   CCTCCGCCTGCACTTGCCTGTGT             46 bp, e1-2, 56% GC               growth       TACACTTCCTGCTGCTGTGCTT               factor 18       (SEQ ID NO: 358)               M335   FGF19   fibroblast   NM_005117   GACGGCAAGATGCAGGGGCTG             45 bp, e2-3, 57% GC               growth       TCAGTACTCGGAGGAAGACTGT                         factor 19       (SEQ ID NO: 359)               M336   FGF20   fibroblast   NM_019851   GAATGACAAAGGAGAAGTCTAT             50 bp, e2-3, 40% GC               growth       GATCAGAGAAACTTACTTCCGAA                         factor 20       GCA                       (SEQ ID NO: 360)               M337   FGF21   fibroblast   NM_019113   TGCTGACCAGAGCCCCGAAAGT             45 bp, e1-2, 55% GC               growth       CCTGCAGCTGAAAGGCTTGAA               factor 21       (SEQ ID NO: 361)               M338   FGF22   fibroblast   NM_020637   CCACGGCCAGGACAGCATCCTG             40 bp, e1-2, 62% GC               growth       AGATCCGCTCTGTACAC               factor 22       (SEQ ID NO: 362)               M339   FGF23   fibroblast   NM_020638   TTTCAGAGGCAACATTTTTGGAT             50 bp, e2-3, 44% GC               growth       ACACTATTTCGACCCGGAGAAC                         factor 23       CA                       (SEQ ID NO: 363)               M340   FGFR1   fibroblast   NM_000604   CGACTACTATAAAAAGACAACC             50 bp, e13-14, 50% G                         growth       ACGGCCGACTGCCTGTGAAGTG                         factor       ATGG               receptor                  (SEQ ID NO: 364)               M341   FGFR2   fibroblast   NM_000141   TTCTTGGAGCCTGCACACAGGAT             50 bp, e13-14, 48% G                         growth       GGCCTCTCTATGTCATAGTTGA             detects almost all               factor       AT   variants (tyrosine               receptor                  (SEQ ID NO: 365)   kinase domains)               M342   FGFR3   fibroblast   XM_044120   ACCGTAGCCGTGAAGATGCTGA             49 bp, e10-11, 53% G                         growth       AGACGATGCCACTGACAAGGA                         factor       TGT               receptor                  (SEQ ID NO: 366)               M343   FGFR4   fibroblast   NM_002011   ACTGTGGCCGTCAAGATGCTCAA             48 bp, e11-12, 54% G                         growth       GACAACGCCTCTGACAAGGACC                         factor       G               receptor                  (SEQ ID NO: 367)               M344   FIBP   fibroblast   NM_004214   CACTACTCATCGAGAGGTACTAT             50 bp, 50% GC               growth       CCTTTGATGAGGCCTITTGTTCGG                         factor (acidic)       AG               intracellular       (SEQ ID NO: 368)               binding protein               M345   SPRY1   sprouty homolog   XM_036349   CTGTTCACAATCACACTGCTGCT             50 bp, 48% GC               1, antagonist       TAGATACCTGTGTATGGGAGCCA                         of FGF       GT               signaling       (SEQ ID NO: 369)               M346   SPRY2   sprouty homolog   NM_005842   CCCAGAACGTGATTGACTATGG             50 bp, 48% GC               2, antagonist       ACTTGTGTATGCTGTGTGAAAG                         of FGF       CTC               signaling       (SEQ ID NO: 370)               M347   IGF1R   insulin-like   NM_000875   TACCGGCACAATTACTGCTCCAA             50 bp, e9-10, 46% GC                         growth factor       GACAAAATCCCCATCAGGAAGT                         1 receptor       TGC                       (SEQ ID NO: 371)               M348   IGF2   insulin-like   NM_000612   TCGACCCCTCCGACCGTGCTTC             41 bp, e3-4, 61% GC               growth factor       GACAACTTCCCCAGATA               2       (SEQ ID NO: 372)               M349   IGF1   insulin-like   X00173   GACATGCCCAAGACCCAGAAGG             50 bp, e3-4, 48% GC               growth factor       AGTACATTTGAAGAACGCAAGT                         1       GAGG                       (SEQ ID NO: 373)               M350   IGFBP1   insulin-like   NM_000596   GAATGGATTTTATCACAGCAGA             50 bp, e2-3, 47% GC               growth factor       GTGTGAGACATCCATGGATGGA                         binding protein       AGGC                       (SEQ ID NO: 374)               M351   IGFBP2   insulin-like   NM_000597   AAGCATGGCCTGTACAACCTCA             50 bp, e3-4, 50% GC               growth factor       ACAGTGCAAGATGTCTCTGAAC                         binding protein       GGCA                       (SEQ ID NO: 375)               M352   IGFBP3   insulin-like   NM_000598   AAGCGGGAGACAGAATATGG   50 bp, e2-3, 50% GC               growth factor       TCCCTGCCGTAGAGAAATGGAA                         binding protein       ACACACT                       (SEQ ID NO: 376)               M353   IGFBP4   insulin-like   NM_001552   AACGGCAACTTCCACCCCAAGC             42 bp, e2-3, 57% GC               growth factor       GTGTCACCCAGCTCTGGAT               binding protein       (SEQ ID NO: 377)               M354   IGFBP5   insulin-like   U20271   CAAAGGATTCTACAAGAGAAAG             49 bp, e3-4, 51% GC               growth factor       AGTGCAAACCTTCCCGTGGCG                         binding protein       AG                       (SEQ ID NO: 378)               M355   IGFBP6   insulin-like   NM_002178   GCGCGCCTGCTGTTGCAGAGGA             42 bp, e1-2, 54% GC               growth factor       AATCCTAAGGAGAGTAAAC               binding protein       (SEQ ID NO: 379)               M356   IMP-1   IGF-II mRNA-   AF117106   ATGGTACAGTAGAGAACTGTGA             50 bp, e2-3, 50% GC               binding protein       CAAGTGAACACCGAGAGTGAGA                                 GGCA                       (SEQ ID NO: 380)               M357   IMP-2   IGF-II mRNA-   NM_006548   GGACAGTGGAGAATGTGGAACA             50 bp, e4-5, 50% GC               binding protein       GTCAACACAGACACAGAAACCG                                 CGTT                       (SEQ ID NO: 381)               M358   IMP-3   IGF-II mRNA-   AF117108   TATCCCGCCTCATTTACAGTGG             50 bp, e2-3, 48% GC               binding protein       GGTGCTGGATAGTTTACTAGTC                                 GT                       (SEQ ID NO: 382)               M359   NGFB   nerve growth   NM_002506   GTTCTACACTCTGATCACAGCTT             50 bp, 50% GC               factor, beta       TCTGATCGGCATACAGGCGGAA                         polypeptide       CAC                       (SEQ ID NO: 383)               M360   NGF-2       X53655   CAAGCTCTCCAAGCAGATGGTG             50 bp, 50% GC                       ACGTTAAGGAAAATTACCAGAG                                 ACCC                       (SEQ ID NO: 384)               M361   NGFR   nerve growth   NM_002507   TTGTGGCCTACATAGCCTTCAA             50 bp, e4-5, 50% GC               factor       GGTGGAACAGCTGCAAGCAGAA                         receptor       AAG                       (SEQ ID NO: 385)               M362   TDGF1   teratocarcinoma   NM_003212   TCTTTGAACTGGGATTAGTTGC             44 bp, e2-3, 50% GC               derived growth       GGCTGGGCCATCAGGAATTT               factor 1       (SEQ ID NO: 386)               M363   PDGFA   platelet-   NM_002607   AGATAGACTCCGTAGGGAGTGA             48 bp, e3, 52% GC               derived growth       GATTCTTTGGACACCAGCCTGA                         factor alpha       G               polypeptide       (SEQ ID NO: 387)               M364   PDGFB   platelet-   NM_002608   GACCTGTCCAGGTGAGAAAGAT             47 bp, e4-5, 51% GC               derived growth       GAGATTGTGCGGAAGAAGCCAA                         b polypeptide       C                       (SEQ ID NO: 388)               M365   PDGFC   platelet-   NM_016205   TTTCCAGCAACAAGGAACAGAA             50 bp, e1-2, 46% GC               derived growth       GGAGTACAAGATCCTCAGCATG                         factor C       GAGA                       (SEQ ID NO: 389)               M366   PDGFD   platelet-   AF336376   TCATACCATGACCGGAAGTCAA             50 bp, e5-6, 44% GC               derived growth       AGTTGACCTGGATAGGCTCAAT                         factor D       TGA                       (SEQ ID NO: 390)               M367   PDGFRA   platelet-   NM_006206   TCAATGGACTTACCCTGGAGAA             50 bp, e5-6, 48% GC               derived growth       GAAAGGCAAAGGCATCACAAT                         factor receptor,       TGG               alpha poly-       (SEQ ID NO: 391)               peptide               M368   PDGFRB   platelet-   NM_002609   TGCCTACTATGTCTACAGACTC             50 bp, e4-5, 48% GC               derived growth       GGTGTCATCCATCAACGTCTCT                         factor receptor,       GA               beta polypeptide       (SEQ ID NO: 392)               M369   VEGF   vascular   NM_003376   TGAGGAGTCCAACATCACCATG             50 bp, e3-4, 48% GC               endothelial       AGATTATGCGGATCAAACCTCA                         growth factor       AAG                       (SEQ ID NO: 393)               M370   VEGFB   vascular   NM_003377   ACCAGAGGAAAGTGGTGTCATG             50 bp, 50% GC               endothelial       ATAGATGTGTATACTCGCGCTA                         growth factor       TGC               B       (SEQ ID NO: 394)               M371   VEGFC   vascular   NM_005429   CAGCACGAGCTACCTCAGCAAG             50 bp, e1-2, 46% GC               endothelial       CGTTATTTGAAATTACAGTGCCT                         growth factor       TCT               C       (SEQ ID NO: 395)               M372   VEGFD   vascular   NM_004469   CAGTAATGAACATGGACCAGTG             50 bp, e1-2, 46% GC               endothelial       AGCGATCATCTCAGTCCACATT                         growth factor       AAC               D       (SEQ ID NO: 396)               M373       similar to   XM_067723   TTTGCAGAACTTGTGGAAAAAC             50 bp, e4-5, 34% GC               Vascular       GAAAATAGTGGGTTTACATACT                         endothelial       AC               growth factor       (SEQ ID NO: 397)               receptor               M374   ADAR   adenosine   NM_001111   AAAGAAGGCCCTGCCCATGAA             48 bp, e4-5, 50% GC               deaminase, RN       CAAGTTCCAATACTGTGTTGCA             should detect all 3               specific       G   variants                       (SEQ ID NO: 398)               M375   SPS   selenium donor   NM_012247   ATCGTAGACGACCCTTACATGAT             48 bp, e2-3, 52% GC               protein       GGCAGGATAGCGTGTGCCAATG             may also detect                       C   LOC158861                       (SEQ ID NO: 399)               M376   SPS2   selenium donor   NM_012248   ATGTTACTCAGCGTCAGCCAGA             50 bp, 50% GC               protein 2       ATGAGTGAGGAGGAACGCGAAA                                 GGT                       (SEQ ID NO: 400)               M377   LOC1235   similar to   XM_063731   GAACTGAAGGGCACAGGCTGCA             50 bp, e1-2, 50% GC               Selenide, water       AGAGAACCACTTCCAAGAAGAT                         dikinase 1       AGCA                       (SEQ ID NO: 401)               M378   LOC1295   similar to   XM_065297   TTAAAGATGCAGCCGAGGAAG             50 bp, e1-2, 42% GC               selenophosphat                 GGAATATGTAGAGTTGACATAC                         synthetase       AAGA                       (SEQ ID NO: 402)               M379   LOC1505   -//-   XM_086946   AAAATATGAATCCCACACCTGG             50 bp, e1-2, 42% GC                       CCACCTCTTAATCTAGACAGAAA                                 AGC                       (SEQ ID NO: 403)               M380   LOC1588   -//-   XM_005845   CAGAATGTGACAATATGCTGAT             50 bp, 40% GC                       TCCTTGGAGTCAGTAATAAAAT                                 CC                       (SEQ ID NO: 404)               M381   LOC1670   -//-   XM_094245   TTACAGTGCTTTTGCTGAAAAT             50 bp, e2-3, 38% GC                       GAATGCAGGAACTGAGACCAT                                 TTA                       (SEQ ID NO: 405)               M382   LOC1684   -//-   XM_095122   CAGAATGTGACAATATGCTGAT             50 bp, 40% GC                       TCCTTGGAGTCAGTAATAAAAT                                 CC                       (SEQ ID NO: 406)               M383   LOC2020   -//-   XM_116312   ATATCACAGGCTTTGGCATTCTA             50 bp, 46% GC                       GACACTCTCAGAACCTCGTGAA                                 AA                       (SEQ ID NO: 407)               M384   SCYA21   small   NM_002989   AAAGGAAAGGGCTCCAAAGGCT             44 bp, e3-4, 54% GC               inducible       CAAGAGGACTGAGCGGTCACA               cytokine       (SEQ DI NO: 408)               subfamily A               M385   ACVR1   activin A   NM_001105   CCAATGTTGGAGACAGCACTTTA             50 bp, e4-5, 40% GC               receptor type I       CAGATTTATTGGATCATTCGTGT                                 CA                       (SEQ ID NO: 409)               M386   ACVR1B   activin A   NM_004302   AGATTGCTCGAAGATGCAATTCT             50 bp, e7-8, 44% GC               receptor type IB       GAGGAGTCCATGAAGAATATCA             should detect only                       CTG   isoform a                       (SEQ ID NO: 410)               M387   ACVR2   activin A   NM_001616   GCTGATCACAGCATTTCATGAAA             50 bp, e4-5, 40% GC               receptor type 2       GGGTTCACTATCAGACTTTCTTA                                 GG                       (SEQ ID NO: 411)               M388   ACVR2B   activin A   NM_001106   TGCTGGCTAGATGACTTCAACT             45 bp, e2-3, 51% GC               receptor type 2B       TACGATAGGCAGGAGTGTGTG                       (SEQ ID NO: 412)               M389   INHBA   inhibin, beta   XM_042996   ATCATCACGTTTGCCGAGTCAG             47 bp, e1-2, 53% GC;               A       ACAGCCAGGAAGACGCTGCACT             same as erythroid                       (SEQ ID NO: 413)   differentiation                           protein               M390   INHBB   inhibin, beta   NM_002193   AATCATCAGCTTCGCCGAGACA             40 bp, e1-2, 60% GC               B       ATGGCCTCGCCTCCTCC                       (SEQ ID NO: 414)               M391   INHBC   inhibin, beta   NM_005538   GAAATCATCAGCTTTGCTGAGA             49 bp, e1-2, 51% GC               C       GGCCTCTCCACCATCAACCAGA                                 C                       (SEQ ID NO: 415)               M392   BLC   B lymphocyte   AF044197   ATGGTTGTCCAAGAAAAGAAAT             50 bp, e3-4, 34% GC               chemo-       ATAGTCTGGAAGAAGAACAAGT                         attractant       AATT                       (SEQ ID NO: 416)               M393   TNFSF13   tumor necrosis   NM_006573   GCCGTTCAGGGTCCAGAAGAAA             50 bp, e2-3, 50% GC               factor (ligand)       AGTCACTCAAGACTGCTTGCAA             AF132600, AF11645                         superfamily,       GAT   AF186114, AF13471                         member 13b       (SEQ ID NO: 417)               M394   TNFRSF5   tumor necrosis   NM_001250   AAACAGTCAGTGCTGTTCTTTGT             50 bp, e2-3, 50% GC           (CD40L)   factor receptor       CCAGCCAGGACAGAAACTGGTG                         superfamily,       GTG               member 5       (SEQ ID NO: 418)               M395   CNTF   ciliary   NM_000614   CTGACTGCTCTTACGGAATCCTA             50 bp, e1-2, 48% GC               neurotrophic       GTGAAGCATCAGGGCCTGAACA                         factor       GAA                       (SEQ ID NO: 419)               M396   CNTFR   ciliary   NM_001842   ATCACCTTTGACGAGTTCACCAT             50 bp, e5-6, 44% GC               neurotrophic       GTGAAGCCTGATCCTCCAGAAA                         factor receptor    GT                       (SEQ ID NO: 420)               M397   CSF1   colony   NM_000757   ACATTTGAGTTTGTAGACCAGGA             50 bp, 44% GC =               stimulating       CAGTTGAAAGATCCAGTGTGCTA             CSF4?               factor I       CT                       (SEQ ID NO: 421)               M398   CSF1R   colony   NM_005211   CTTCCTCCAACACAACAACACTA             50 bp, e4-5, 50% GC               stimulating       GCTCGCAATCCCTCAACAATCT                         factor 1       CT               receptor       (SEQ ID NO: 422)               M399   CSF2   colony   NM_000758   TGAACCTGAGTAGAGACACTGC             50 bp, e1-2, 44% GC               stimulating       GCTGAGATGAATGAAACAGTAG                         factor 2       AGTC                       (SEQ ID NO: 423)               M400   CSF2RA   colony   NM_006140   CTAGTCTCAATGTGAGGTTTGA             50 bp, 46% GC               stimulating       CCAGGACGATGAATTTAAGCTG                         factor 2       GAC               receptor,       (SEQ ID NO: 424)               alpha low-               affinity               M401   CSF2RB   colony   NM_000395   GGCAGAGAAACACATAAAGAG             49 bp, e7-8, 51% GC               stimulating       CAGTGAACATCCAGATGGCCCC                         factor 2       CA               receptor,       (SEQ ID NO: 425)               beta low-               affinity               M402   CSF3   colony   NM_000759   AGGAGAAGCTGGTGAGTGAGT             43 bp, e2-3, 55% GC               stimulating       GCCACCTACAAGCTGTGCCA               factor 3       (SEQ ID NO: 426)               M403   CTF1   cardiotrophin 1   XM_096076   CATGTGTACATCTCAGCCTTAT             50 bp, 48% GC                       CAAGGAGGTGACACCTTCTCTC                                 TG                       (SEQ ID NO: 427)               M404   CLC   cardiotrophin-   AY049779   AAGCCTCAATGACAAACTGCGG             42 bp, 54% GC               li          cytokine       TGACCCAGAACTACGAGGC                       (SEQ ID NO: 428)               M405   EMAPII   small inducible   NM_004757   CAGGCAGAAATTCAAAATGGAG             50 bp, e2-3, 40% GC           (SCYE1)   cytokine       GAAGCAAATAGCATTTCCATCT                         subfamily E,       TAC               member 1       (SEQ ID NO: 429)               M406   Epo   erytropoetin   X02157   CCGAGAATATCACGACGGGCTG             49 bp, e2-3, 51% GC                       GCTGAACACTGCAGCTTGAATGA                                 GAA                       (SEQ ID NO: 430)               M407   CCR3   CC chemokine   AF247361   CATGGCATGTGTAAGCTCCTCT             50 bp, 48% GC               receptor 3       GGGTTTTATCACACAGGCTTGTA                                 AG                       (SEQ ID NO: 431)               M408   eotaxin   eosinophil-   D49372   TGGCAAATGTCCCCAGAAAGCT             50 bp, e2-3, 50% GC               selective CC       GATCTTCAAGACCAAACTGGCC                         chemokine       AGG                       (SEQ ID NO: 432)               M409   osteopont             osteopontin   AF052124   CTAGGCATCACCTGTGCCATAC             50 bp, e2-3, 48% GC                       GTTAAACAGGCTGATTCTGGAA                                 TC                       (SEQ ID NO: 433)               M410   GSN   gelsolin   NM_000177   AAGATGGGAAAATCTTTGTCTG             50 bp, e7-8, 48% GC                       AAGGCAAGCAGGCAAACACGGA                                 GAG                       (SEQ ID NO: 434)               M411   GZMB   granzyme B   NM_004131   AGAGATTAAAAAGACTTCCTTTA             50 bp, e3-4, 46% GC                       GGGGGACTCTGGAGGCCCTCTT                                 GT                       (SEQ ID NO: 435)               M412   CD44   CD44 hyaluronic   X55150   GACAGGACCTCTTTCAATGACAA             50 bp, e5-6, 46% GC               receptor       GCAGCAGAGTAATTCTCAGAGC                         (epithelial       CT               form)       (SEQ ID NO: 436)               M413               AGCACAGACAGAATCCCTCGTA             50 bp, e4-5, 46% GC                       CAATATGGACTCCAGTCATAGTA                                 AAC                       (SEQ ID NO: 437)               M414   IFN-alpha   interferon   J00210   ATTCTGCACCGAACTCTACCAG             50 bp, 50% GC will a                         alpha       GCTGAATGACTTGGAAGCCTGT             detect IFN-alpha 13,                                  GA   may detect 2, F, 6, 5,                                  (SEQ ID NO: 438)               M415   IFN-alpha   interferon   X02959   TGCCTGAAGGACAGACATGACT             50 bp, 46% GC; may           14   alpha       GAATTTCCCCAGGAGGAATTTGA             also detect IFN-alpha                       GG   1B, 21, 17, 5, F, 1B,                       (SEQ ID NO: 439)   D, 2, 21               M416   IFN-alpha   interferon   X02957   ACAAAGGATTCATCTGCTGCTT             50 bp, 46% GC; may           16   alpha       GATGAGACCCTCCTAGACAAAT             also detect IFN-alpha                       TA   14, F, 5, 6, 7, 8,                       (SEQ ID NO: 440)               M417   IFN-alpha   interferon   X02955   AATACAGCCCTTGTGCCTGGGA             50 bp, 46% GC; will           4b   alpha       TTGTCAGAGCAGAAATCATGAG             also detect IFN-alpha                       TCC   21, 7, 13, 14, 1, 16, 1                                 (SEQ ID NO: 441)   d, N, F, H, N, M1, A               M418   IFN-alpha   interferon   X02956   GTAACAGGAGGACTTTGATGAT             50 bp, 40% GC; may               alpha       ATGGCACAAATGGGAAGAATCT             also detect IFN-alpha                       TCCT   14, H                       (SEQ ID NO: 442)               M419   IFN-alpha   interferon   X02958   ATGACTTCAGATTTCCCCAGGA             50 bp, 48% GC; may               alpha       AGTTTGATGGCAACCAGTTCCA             also detect IFN-alpha                       AG   16, F                       (SEQ ID NO: 443)               M420   IFN-alpha   interferon   X02960   AGCCTGCGTAATAGGAGGGCCT             50 bp, 50% GC; may               alpha       GATACTCCTGGCACAAATGGGA             also detect IFN-alpha                       GAAT   21, 17, 4, A, N, F, C,                       (SEQ ID NO: 444)   10, 8               M421   IFN-alpha   interferon   X03125   GAGATGATCCAGCAGACCTTCA             50 bp, 50% GC; will               alpha       CCTCTTCAGCACAAAGGACTCAT             also detect IFN-alpha                       TGC   1B, 201, may detect                                  (SEQ ID NO: 445)   21, 5, 7, 17, 4, 16, 10               M422   IFNAR1   interferon   NM_000629   GACTCATTTACACCATTTCGCAA             50 bp, e3-4, 44% GC               (alph       GCTCAGATTGGTCCTCCAGAAGT                         beta and       CA               omega       (SEQ ID NO: 446)               receptor 1               M423   IFNAR2   interferon   NM_000874   CACTTAATTTGGTTCTCATGGTG             50 bp, e3-4, 38% GC;               (alph       ATATCAGCCTCGTGTTTGGTATT             will detect both soluble               beta and       CA   and membrane-bound               omega       (SEQ ID NO: 447)   receptors               receptor 2               M424   IFN-beta   fibroblast   M35591   GATCAAGCAACTCCAGCAATTC             50 bp, 46% GC               interferon       GAAAGAAGACGCAGCTCTGACT                                 TCT                       (SEQ ID NO: 448)               M425   IFN-beta   interferon-   X04430   ATGCTTCCAATCTGGATTCAAT             50 bp, e3-4, 42% GC =           (IL6)   beta-       GGAGACTTGCCTGGTGAAAATC             IL6               (interleukin 6)       CA                       (SEQ ID NO: 449)               M426   IFMBR   interferon   A26595   GACTCATTTACACCATTTCGCAA             50 bp, e3-4, 44% GC               beta       GCTCAGATTGGTCCTCCAGAAGT                         receptor       CA                       (SEQ ID NO: 450)               M427       IFN-gamma   A25270   ACCTTCTAAGTCACGTCACCAT                         antagonist   NM_006083   GATGCCAAGGGAGTACAATGAG                         cytokine       ATG                       (SEQ ID NO: 451)               M428   IFNG   interferon,   NM_000619   CTTAGGCATTTTGAAGAATTGGA             50 bp, e2-3, 36% GC               gamma       AGAGGAGAGTGACAGAAAAATA                                 TGC                       (SEQ ID NO: 452)               M429   IFNGR1   interferon   NM_000416   GTATGTGAGAATGAACGGAAGT             50 bp, e4-5, 40% GC               gamma       AGATCCAGTATAAAATACTCAC                         receptor 1       AGA                       (SEQ ID NO: 453)               M430   IFNGR2   interferon   NM_005534   TTGTCTACCGAGTGCAGTTTTAAA             50 bp, e2-3, 46% GC               gamma       ACACCGACAGTAAATGGTTCAC                         receptor 2       GCC                       (SEQ ID NO: 454)               M431   ILI8   interleukin   NM_001562   GATATGACTGATTCTGACTGTA             50 bp, e2-3, 40% GC               18       GATAATGCACCCCGGACCATATT                                 AT                       (SEQ ID NO: 455)               M432   IL1A   interleukin   NM_000575   GCATTACATAATCTGGATGAAG             50 bp, e4-5, 36% GC               1, alpha       GTGAAATTTGACATGGGTGCTTA                                 AA                       (SEQ ID NO: 456)               M433   IL1B   interleukin   NM_000576   CCAGTGAAATGATGGCTTATTA             50 bp, e2-3, 40% GC               1, b                 GTGGCAATGAGGATGACTTGTT                                 TT                       (SEQ ID NO: 457)               M434   IL1E   interleukin-1   AF206696   GATGGTGGAGGAAGGGCCGTCT             50 bp, e2-3, 46% GC               epsilon       TCAATCAATGTGTAAACCTATTA                                 TGG                       (SEQ ID NO: 458)               M435   IL1R1   interleukin 1   NM_000877   GTAATAGAATTTATTACTCTAGA             50 bp, e4-5, 36% GC               receptor,       GAAAACAAACCCACAAGGCCTG                         type I                 GAT                       (SEQ ID NO: 459)               M436   IL1R2   interleukin 1   NM_004633   CTGGCACCTACGTCTGCACTACT             50 bp, e2-3, 44% GC               receptor,       GAAATGCTTCTTACTGTGACAAA                         type I                 TG                       (SEQ ID NO: 460)               M437   IL10   interleukin 10   NM_000572   CTTCAGCAGAGTGAAGACTTTCT             50 bp, e1-2, 42% GC                       TCAAATGAAGGATCAGCTGGAC                                 ACT                       (SEQ ID NO: 461)               M438   IL10RA   interleukin 10   NM_001558   TACCTGCTATGAAGTGGCGCTC             50 bp, e2-3, 50% GC               receptor, alpha       GAGGTATGGAATAGAGTCCTGG                                 ACT                       (SEQ ID NO: 462)               M439   IL10RB   interleukin 10   NM_000628   TTCTCTTCCACAGCACCTGAAA             50 bp, e6-7, 44% GC               receptor, beta       GTTTTTGGGCCATCCTCATCATA                                 CA                       (SEQ ID NO: 463)               M440   IL11   interleukin 11   NM_000641   GACATGAACTGTGTTTGCCGCCT             40 bp, e1-2, 57% GC                       GTCCTGGTCGTGCTGA                       (SEQ ID NO: 464)               M441   IL10RA   interleukin 10   NM_004512   TATGAGAACTTCTCTTGCACTTG             49 bp, e5, 49% GC               receptor, alpha       AGTCCCAGCCAGATCAGCGGTT                                 C                       (SEQ ID NO: 465)               M442   IL12   interleukin 12   AF180562   GGCCGTCAGCAACATGCTCCAG             50 bp, e2-3, 50% GC                       AGGCCAGACAAACTCTAGAATT                                 ACC                       (SEQ ID NO: 466)               M443   IL12RB1   interleukin 12   NM_005535   TGACCCTGCAGCTCTACAACTCA             50 bp, e4-5, 50% GC               receptor, beta       TTAAATATGAGCCTCCTCTGGGA                         1       A                       (SEQ ID NO: 467)               M444   IL12RB2   interleukin 12   NM_00155   AGTAACAGCAGGCTCTGGAATA             50 bp, e6-7, 42% GC               receptor, beta       GGTTAATGTTACAAAGGCCAAA                         2       GAAG                       (SEQ ID NO: 468)               M445   IL13   interleukin 13   NM_002188   ATCACCCAGAACCAGAAGGCTC             46 bp, e1-2, 54% GC                       GCTCTGCAATGGCAGCATGGTAT                                 (SEQ ID NO: 469)               M446   IL13RA1   interleukin 13   NM_001560   CCAGAATTTGAGAGAAATGTGG             50 bp, e6-7, 42% GC               receptor, alpha       GAATACATCTTGTTTCATGGTC                                 TGG                       (SEQ ID NO: 470)               M447   IL13RA2   interleukin 13   NM_000640   TGGAGTGATAAACAATGCTGGG             50 bp, e8-9, 40% GC               receptor, alpha       AGGTGAAGACCTATCGAAGAAA                                 CTTT                       (SEQ ID NO: 471)               M448   IL15   interleukin 15   NM_000585   CATTCATGTCTTCATTTTGGGCT             50 bp, e4-5, 44% GC                       TTCAGTGCAGGGCTUCCTAAAA                                 G                       (SEQ ID NO: 472)               M449   IL15RA   interleukin 15   NM_002189   TTCTGGAAAAGAGCCCGCAGCT             47 bp, e3-4, 51% GC               receptor, alpha       ATCTCCCAGCTCAAACAACACA                                 (SEQ ID NO: 473)               M450   IL2   interleukin 2   NM_000586   ATTTAAGTTTTACATGCCCAAGA             50 bp, e2-3, 40% GC                       GGCCACAGAACTGAAACAGCTT                                 AGT                       (SEQ ID NO: 474)               M451   IL2RA   interleukin 2   NM_000417   AACCAATGTCAATGCACAAGCT             50 bp, e2-3, 44% GC               receptor, alpha       GCCACTCGGAACACAACGAAA                                 AGT                       (SEQ ID NO: 475)               M452   IL2RB   interleukin 2   NM_000878   AACCTGATCCTCGGAGCCCCAGA             50 bp, e4-5, 50% GC               receptor, beta       TCTCAGAAACTGACCACAGTTGA                                 AT                       (SEQ ID NO: 476)               M453   IL2RG   interleukin 2   NM_000206   TGGGAATGAAGACACCACAGCT             50 bp, e1-2, 50% GC               receptor, gamma       ATTTCTTCCTGACCACTATGCCC                                 CTG                       (SEQ ID NO: 477)               M454   IL3   interleukin 3   NM_000588   ATCAGCAATTGAGAGCATTCTTA             50 bp, e3-4, 46% GC                       AAATCTCCTGCCATGTCTGCCC                                 GG                       (SEQ ID NO: 478)               M455   IL3RA   interleukin 3   NM_002183   TTGCCAACAGGCGTCAACAGTA             50 bp, 48% GC               receptor, alpha       GAGTGTCTTCACTACAAAACGG                                 GCT                       (SEQ ID NO: 479)               M456   IL4   interleukin 4   NM_000589   TAACAGACATCTTTGCTGCCTC             50 bp, e2-3, 44% GC                       AGAACACAACTGAGAAGGAAA                                 TTC                       (SEQ ID NO: 480)               M457   IL4R   interleukin 4   NM_000418   TTGGAGTGAAAACGACCCGGCA             50 bp, e5-6, 46% GC               receptor       ATTTCAGAATCTATAACGTGAC                                 ACC                       (SEQ ID NO: 481)               M458   IL5   interleukin 5   NM_000879   TCGAACTCTGCTGATAGCCAAT             50 bp, e1-2, 48% GC                       GACTCTGAGGATTCCTGTTCCTG                                 AC                       (SEQ ID NO: 482)               M459   IL5RA   interleukin 5   NM_000564   ACACGCAGTATTTTCTCTACTAT             50 bp, e7-8, 42% GC               receptor, alpha       GGTATGGCTCTTGGACTGAAGAA                                 GC                       (SEQ ID NO: 483)       M460   IFNB1   interferon,   NM_002176   AGTGTCTCCTCCAAATTGCTCTC             50 bp, 48% GC               beta fibroblast       TGTTGTGCTTCTCCACTACAGCT                                 TT                       (SEQ ID NO: 484)               M461   IL6R   interleukin 6   NM_000565   TCAAAGACATTCACAACATGGA             50 bp, e5-6, 46% GC               receptor       GGTCAAGGACCTCCAGCATCAC                                 TGT                       (SEQ ID NO: 485)               M462   IL7   interleukin 7   NM_000880   TAATGGTCAGCATCGATCAATTA             50 bp, e2-3, 38% GC                       TGGACAGCATGAAAGAAATTGG                                 AGC                       (SEQ ID NO: 486)               M463   IL7R   interleukin 7   NM_002185   GGAGAAAGTGGCTATGCTCAAA             50 bp, e1-2, 46% GC               receptor       TGGAGACTTGGAAGATGCAGAA                                 TGGA                       (SEQ ID NO: 487)               M464   IL8   interleukin 8   NM_000584   CTGATTTCTGCAGCTCTGTGTGA             50 bp, e1-2, 48% GC                       GGTGCAGTTTTGCCAAGGAGTG                                 AA                       (SEQ ID NO: 488)               M465   IL8RA   interleukin 8   NM_000634   CTTGGTCAAGTTTGTTTGTCTTG             50 bp, 46% GC               receptor, alpha       CTGCTGGGGACTGTCTATGAAT                                 GT                       (SEQ ID NO: 489)               M466   IL8RB   interleukin 8   NM_001557   GGAAGATTTTAACATGGAGAGT             50 bp, 40% GC               receptor, beta       ACAGCTTTGAAGATTTCTGGAAA                                 GTG                       (SEQ ID NO: 490)               M467   IL9   interleukin 9   NM_000590   ACTAAAGAACAACAAGTGTCCA             50 bp, e4-5, 40% GC                       ATTTTTCCTGTGAACAGCCATG                                 ACC                       (SEQ ID NO: 491)               M468   IL9R   interleukin 9   NM_002186   TGTTCAAGCTGTCGCCCAGGGT             46 bp, e8-9, 50% GC               receptor       AGAGAATCTTCTAGCAGAACGT                                 (SEQ ID NO: 492)               M469   LIF   leukemia   NM_002309   TGCCAATGCCCTCTTTATTCTCT             48 bp, e2-3, 53% GC               inhibitory       TACACAGCCCAGGGGGAGCCGT                         factor       (SEQ ID NO: 493)               receptor               M470   LIFR   leukemia   NM_002310   GGAGCCCTGTGAAGAACATTTCT             50 bp, e6-7, 44% GC               inhibitory       GGATACCTGATTCTCAGACTAA                         factor       TT               receptor       (SEQ ID NO: 494)               M471   BLT1   leukotriene B4   AB008193   TCACTAGGTGTAGAGTTCATCT             50 bp, 48% GC               receptor       CTGCTGGCTATCATCCTGCTGTC                                 GT                       (SEQ ID NO: 495)               M472   BLT2   leukotriene B4   AB008193   TTGGCCTTCTTCAGTTCTAGCGT             44 bp, 52% GC               receptor       AACCCGGTGCTCTACGTCTT                       (SEQ ID NO: 496)               M473   CYSLT1   cysteinyl   NM_006639   CAGGAAATCTGACAGTATCTTCT             50 bp, 44% GC               leukotriene       CCACATGCCATGACACTATTGAT                         receptor 1       AC                       (SEQ ID NO: 497)               M474   LTA4H   leukotriene A4   NM_000895   TGTGAAATTAACCTATACTGCA             50 bp, e4-5, 44% GC               hydrolase       GGTGTCTGTCCCTAAAGAACTG                                 GG                       (SEQ ID NO: 498)               M475   LTC4S   leukotriene C4   NM_000897   GTCTACCGAGCCCAGGTGAACT             36 bp, e2-3, 58% GC               synthase       AGCGAGTACTTC                       (SEQ ID NO: 499)               M476   LTB4DH   NADP depende             XM_088569   ATGATGGGGCAGGAAGTGGCCA             50 bp, e4-5, 46% GC               leukotriene b4                  AGTTGTGGAAAGTAAAAATGTA                         hydroxydehydro-       CCCT               genase       (SEQ ID NO: 500)               M477   SELL   selectin L   NM_000655   TCATCTCCAGAACCAACCTGTCA             50 bp, e5-6, 46% GC                       GTGATTCAGTGTGAGCCTCTAT                                 GC                       (SEQ ID NO: 501)               M478   SCYC1   lymphotactin   NM_002995   TCTCTCACTGCATACATTGTGGA             50 bp, e1-2, 44% GC                       GGTGTAGGGAGTGAAGTCTCAG             also detect SCYC2 =                       TAA   SCYC1?                       (SEQ ID NO: 502)               M479   CCXCR1   chemokine (C   NM_005283   CAAACTCCTCAATATGATCTTCT             50 bp, 44% GC               motif) XC       CATCAGCCTCTACAGCAGCATCT                         receptor 1       CT                       (SEQ ID NO: 503)               M480   SCYA22 =   small inducible   NM_002990   TTCAAGCAACTGAGGCAGGCCC             42 bp, e1-2, 58% GC           MDC?   cytokine       ACGGCGCCAACATGGAA               subfamily A       (SEQ ID NO: 504)               (C                             Cys),               member 2               M481   CCR2   chemokine (C-             NM_000648   AGTGCTTGACTGACATTTACCT             50 bp, 48% GC shoul                         motif) receptor       TCAACCTGGCCATCTCTGATCT             detect both isoforms                       TT   may also detect CCR                       (SEQ ID NO: 505)               M482   SCYA3   small inducible   NM_002983   AAGCCCGGTGTCATCTTCCTAA             41 bp, e2-3, 58% GC               cytokine A3       AAGCGAAGCCGGCAGGT                                 (SEQ ID NO: 506)               M483   SCYA4   small inducible   NM_002984   GCTCCCAGCCAGCTGTGGTATT             50 bp, e2-3, 50% GC               cytokine A4       AAACCAAAAGAAGCAAGCAAGT                                 TGT                       (SEQ ID NO: 507)               M484   AMH   anti-Mullerian   NM_000479   AGCCCCGCGGAGAGGACTCCCG             30 bp, e3-4, 70% GC               hormone       CTGAGTA                       (SEQ ID NO: 508)               M485   AMHR2   anti-Mullerian   NM_020547   ATGAGGAGCGCTGGCAGAATG             48 bp, e7-8, 52% GC               hormone receptor       CAATATAAACCAGGTATTGCCCA                         typeII       C                       (SEQ ID NO: 509)               M486   SCYD1   neurotactin   NM_002996   TGCGGCAAACGCGCAATCATCT             47 bp, e2-3, 55% GC                       GAGACGAGACAGCACAGGCTGT                                 (SEQ ID NO: 510)               M487   NRTN   neurturin   NM_004558   TCAGTGCTCTGCAGCTCCGTGCT             40 bp, e1, 57% GC                       TCCATCTGGATGTGTC                       (SEQ ID NO: 511)               M488   BMP-8   bone morpho-   NM_001720   AGGTGGTCCAGGAGCAGTCCAA             50 bp, e2-3, 50% GC               genetic       AGGGAGTCTGACTTGTTCTTTTT                         protein 8       GAT                       (SEQ ID NO: 512)               M489   FAAH   fatty acid   NM_001441   TGCTCTTCACCTATGTGGGAAA             49 bp, e2-3, 51% GC               amide hydrolase       CCTGGGAAGTGAACAAAGGGA                                 AA                       (SEQ ID NO: 513)               M490   OSM   oncostatin M   NM_020530   ATCTCATGCAGGACACCAGCAG             50 bp, e2-3, 50% GC                       CTCCTGGACCCCTATATACGTAT                                 CAA                       (SEQ ID NO: 514)               M491   OSMR   oncostatin M   NM_003999   TCCTTGAGGACTTACCAGAGTGA             50 bp, e2-3, 46% GC               receptor       GTCTTGGCTGAACGTTTACCATT                                 AC                       (SEQ ID NO: 515)               M492   SCYA5   RANTES   NM_002985   AAGTGCTCCAACCCAGCAGTCG             44 bp, e2-3, 54% GC                       TTTGTCACCCGAAAGAACCG                       (SEQ ID NO: 516)               M493       RANTES   L10918   TGTACTCCTTGGTATTTGTCATT             50 bp, 46% GC               Receptor       GCCTGGTTGGAAACATCCTGGT                                 TC                       (SEQ ID NO: 517)               M494       relaxin H1   X00949   CCTCAGACACCTAGACCAGTGG             50 bp, e1-2, 46% GC                       AGAAATTGTACCATCCTTCATCA             should detect also                       CAA   relaxin H2 (1 bp                       (SEQ ID NO: 518)   different)               M495   SDF1   stromal cell-   XM_165565   CTCCAAACTGTGCCCTTCAGATT             50 bp, e2-3, 50% GC               derived factor       TAGCCCGGCTGAAGAACAACAA                         1       AGA                       (SEQ ID NO: 519)               M496   SDF2   stromal cell-   NM_006923   CTTCACCTCTTTCTGGAAACCA             50 bp, e2-3, 48% GC               derived factor       AAGTGAGTGCTTTTGGTGAGGA                                 GGT                       (SEQ ID NO: 520)               M497   SDFR1   stromal cell   XM_015963   AGAATGCCAGCAACATGGAGTA             50 bp, e3-4, 48% GC               derived factor       AGGATCAATAAGCCGAGAGCTG                         receptor 1       GGAT                       (SEQ ID NO: 521)               M498   TGF-alpha   transforming   M31172   TTTTTGGTGCACGAGGACAAGC             48 bp, e2-3, 52% GC               growth factor-       GCATGTGTCTGCCATTC TGGGTA                         alpha       (SEQ ID NO: 522)               M499   TGFB1   transforming   NM_000660   GTGCTAATGGTGGAAACCCACA             50 bp, e1-2, 44% GC               growth factor,       CGAAATCTATGACAAGTTCAAG                         beta 1       AGAG                       (SEQ ID NO: 523)               M500   TGFB2   transforming   M19154   GAAGAACTAGAAGCAAGATTTG             50 bp, e3-4, 42% GC               growth factor,   M22045   AGGTATTGATGGCACCTCCACAT                         beta 2   M22046   TAG                       (SEQ ID NO: 524)               M501   TGFB3   transforming   J03241   GAGGTGATGGAAATCAAATTCA             50 bp, e4-5, 46% GC               growth factor,       AGGCGTGGACAATGAGGATGA                         beta 3       ATGG                       (SEQ ID NO: 525)               M502   TGFBR1   transforming   NM_004612   ATGACAACGTCAGGTTCTGGCT             50 bp, e3-4, 46% GC               growth factor,       GGTTTACCATTGCTTGTTCAGAG                         beta receptor       AC               1       (SEQ ID NO: 526)               M503   TGFBR2   transforming   NM_003242   CACAGGAAGTCTGTGTGGCTGTA             50 bp, e2-3, 44% GC               growth factor,       GGAGAAAGAATGACGAGAACAT                         beta receptor       ACA               2       (SEQ ID NO: 527)               M504   TGFBR3   transforming   NM_003243   TGGGGTCTCCAGACTGTTTTTGG             50 bp, e3-4, 50% GC               growth factor,       GTCTGAGGGTTCTGTGGTCCAGT                         beta receptor       TT               3       (SEQ ID NO: 528)               M505   THPO   thrombopoietin   NM_000460   TTTAGCTTGGGAGAATGGAAAA             50 bp, e3-4, 50% GC                       CCAGATGGAGGAGACCAAGGCA                                 AGGA                       (SEQ ID NO: 529)               M506   TNF   tumor necrosis   NM_000594   CGAGTGACAAGGCTGTAGCCCA             46 bp, e2-3, 52% GC           TNFSF2   factor       GTTGTAGCAAACCCTCAAGCTGA                                 (SEQ ID NO: 530)               M507   TNFSF1   lymphotoxin   NM_000595   AAACCTGCTGCTCACCTCATTG             45 bp, e3-4, 53% GC           (LTA)   alpha       GACCCCAGCAAGCAGAACTCA                       (SEQ ID NO: 531)               M508   TNFSF3   lymphotoxin   NM_002341   CTTAGTGCCCCAGGATCAGGGA             39 bp, e1-2, 59% GC           (LTB)   be                 GACTGGTAACGGAGAC                       (SEQ ID NO: 532)               M509   TNFSF4   tumor necrosis   NM_003326   TGCCTGCACTTCTCTGCTCTTCA             50 bp, e1-2, 48% GC               factor (ligand)       GTATCACATCGGTATCCTCGAAT                         superfamily,       CA               member 4       (SEQ ID NO: 533)               M510   TNFSF5   tumor necrosis   NM_000074   GAGGCCAGCAGTAAAACAACAT             50 bp, e2-3, 50% GC               factor (ligand)       TGTGTTACAGTGGGCTGAAAAA                         superfamily,       GATA               member 5       (SEQ ID NO: 534)               M511   TNFSF6   tumor necrosis   NM_000639   GAGCTGAGGAAAGTGGCCCATT             50 bp, e3-4, 50% GC               factor (ligand)       AACAGGCAAGTCCAACTCAAGG                         superfamily,       CCAT               member 6       (SEQ ID NO: 535)               M512   TNFSF7   tumor necrosis   NM_001252   TGCAGCTGAATCACACAGGACC             43 bp, e2-3, 55% GC =               factor (ligand)       AGCAGGACCCCAGGCTATA   CD27?               superfamily,       (SEQ ID NO: 536)               member 7               M513   TNFSF8   tumor necrosis   NM_001244   ATTATGGTGTTGGTCGTTCAGA             50 bp, e1-2, 50% GC               factor (ligand)       ACGGACTCCATTCCCAACTCAC                         superfamily,       GA               member 8       (SEQ ID NO: 537)               M514   TNFSF9   tumor necrosis   NM_003811   AGGGCATGTTTGCGCAGCTGGT             50 bp, e2-3, 50% GC               factor (ligand)       CCCAAAATGTTCTGCTGAT               superfamily,       (SEQ ID NO: 538)               member 9               M515   TNFSF10   tumor necrosis   NM_003810   GTACTTTACCAACGAGCTGAAG             50 bp, e1-2, 46% GC               factor (ligand)       GATGCAGGACAAGTACTCCAAA                         superfamily,       GTG               member 10       (SEQ ID NO: 539)               M516   TNFSF11   tumor necrosis   NM_003701   GCCTTTCAAGGAGCTGTGCAAA             50 bp, e2-3, 42% GC               factor (ligand)       GGAATTACAACATATCGTTGGAT                         superfamily,       ACA               member 11       (SEQ ID NO: 540)               M517   TNFSF12   tumor necrosis   NM_003809   CTCTACTACCTGTACTGTCAGGT             47 bp, e6-7, 51% GC               factor (ligand)       CACTTTGATGAGGGGAAGGCTG                         superfamily,       (SEQ ID NO: 541)               member 12               M518   TNFSF13   tumor necrosis   NM_003808   TGGAGTTTATCTGCTGTATAGC             50 bp, e4-5, 44% GC               factor (ligand)       GGTCCTGTTTCAAGACGTGACTT                         superfamily,       CA               member 13       (SEQ ID NO: 542)               M519   TNFSF14   tumor necrosis   NM_003807   AGCTGATACAAGAGCGAAGGT             42 bp, e2-3, 54% GC               factor (ligand)       CACGAGGTCAACCCAGCAG               superfamily,       (SEQ ID NO: 543)               member 14               M520   TNFSF15   tumor necrosis   NM_005118   TCACCAAGAACCGAATGAACTA             50 bp, 44% GC               factor (ligand)       ACCAACAAATTCCTGCTGATCC             may also detect               superfamily,       GAG   transmembrane LIGH                         member 15       (SEQ ID NO: 544)   mRNA (AY028261)               M521   TNFSF18   tumor necrosis   NM_005092   AAGGAGCCCTGTATGGCTAAGT             50 bp, e2-3, 44% GC               factor (ligand)       GGACCATTACCCTCAAAATGGCA                         superfamily,       AAT               member 18       (SEQ ID NO: 545)               M522   TNFRSF10B   tumor necrosis   NM_003842   TTTTCTGCTTGCGCTGCACCAGG             50 bp, e2-3, 50% GC               factor receptor       GTGATTCAGGTGAAGTGGAGCT                         superfamily,       AGT               member 10B       (SEQ ID NO: 546)               M523   TNFRSF11A   tumor necrosis   XM_036978   ACAAATGCAGACCCTGGACCAA             50 bp, e4-5, 50% GC               factor receptor       TGTACCTTCCTTGGAAAGAGAGT                         superfamily,       GAA               member 11A       (SEQ ID NO: 547)               M524   TNFRSF11B   tumor necrosis   NM_002546   TGGATTTGGAGTGGTGCAAGCT             50 bp, e2-3, 50% GC               factor receptor       AACCCCAGAGCGAAATACAGTT                         superfamily,       GCA               member 11B       (SEQ ID NO: 548)               M525   TNFRSF12   tumor necrosis   NM_003790   CACACACGGCTACTCTGTTCCC             45 bp, e4-5, 53% GC               factor receptor       AGAGATACTGACTGTGGGA               superfamily,       (SEQ ID NO: 549)               member 12               M526   TNFRSF14   tumor necrosis   NM_003820   GTGAAAAGAAGAAAGCCAAGG             50 bp, e6-7, 48% GC               factor receptor       TGATGTAGTCAAGGTGATCGTCT                         superfamily,       CGT               member 14       (SEQ ID NO: 550)               M527   TNFRSF17   tumor necrosis   NM_001192   CATGTCAGCGTTATTGTAATGCA             50 bp, e1-2, 42% GC               factor receptor       GTGTGACCAATTCAGTGAAAGG                         superfamily,       ACG               member 17       (SEQ ID NO: 551)               M528   TNFRSF18   tumor necrosis   NM_004195   ACTGCAAACCTTGGACAGACTG             49 bp, e3-4, 51% GC               factor receptor       ACCCAGTTCGGGTTTCTCACTGT                         superfamily,       TT               member 18       (SEQ ID NO: 552)               M529   TNFRSF19   tumor necrosis   NM_018647   GTATTACTAGGCTATTTGTCATG   50 bp, e2-3, 38% GC               factor receptor       TAAAGTGACTTGTGAATCAGGAG               superfamily,       ACTG               member 19       (SEQ ID NO: 553)               M530   TNFRSF21   tumor necrosis   NM_014452   ACTTATGTTCCCAAAGGCATGAA   50 bp, e2-3, 44% GC               factor receptor       CTCAACAGAATCCAACTCTTCTG               superfamily,       CCTC               member 21       (SEQ ID NO: 554)               M531   TNFRSF3   tumor necrosis   NM_002342   ACTGAAGCCGAGCTCAAAGATG   50 bp, e4-5, 50% GC               factor receptor       AAGTTGGGAAGGGTAACAACCA               superfamily,       CTGCGT               member 3       (SEQ ID NO: 555)               M532   TNFRSF4   tumor necrosis   NM_003327   TGCACGTGGTGTAACCTCAGAAG   41 bp, e2-3, 56% GC               factor receptor       TGGGAGTGAGCGGAAGCA               superfamily,       (SEQ ID NO: 556)               member 4               M533   TNFRSF6   tumor necrosis   NM_000043   AGATTGTGTGATGAAGGACATGG   50 bp, e3-4, 44% GC =               factor receptor       CTTAGAAGTGGAAATAAACTGCA   Apo1 antigen?               superfamily,       CCCG               member 6       (SEQ ID NO: 557)               M534   TNFRSF6B   tumor necrosis   NM_003823   TCAGCACCAGGGTACCAGGAGC   38 bp, e2-3, 60% GC               factor receptor       TGAGGAGTGTGAGCGT               superfamily,       (SEQ ID NO: 558)               member 6B               M535   TNFRSF7   tumor necrosis   NM_001242   CATCAACGAAGGAAATATAGAT   50 bp, e5-6, 42% GC               factor receptor       CAAACAAAGGAGAAAGTCCTGT               superfamily,       GGAGCC               member 7       (SEQ ID NO: 559)               M536   TNFRSF8   tumor necrosis   NM_001243   CACGGAGCACACCAATAACAAG   50 bp, e9-10,               factor receptor       ATTGAGAAAATCTACATCATGAA   42% GC               superfamily,       GGCTG               member 8       (SEQ ID NO: 560)               M537   TNFRSF9   tumor necrosis   NM_001561   TGTGACATATGCAGGCAGTGTAA   50 bp, e3-4, 48% GC               factor receptor       AGGTGTTTTCAGGACCAGGAAGG               superfamily,       AGTG               member 9       (SEQ ID NO: 561)               M538   GAPD   glyceraldehyde-   NM_002046   CCCTTCATTGACCTCAACTACAT   50 bp, e2-3, 40% GC               3-phosphate       GGTTTACATGTTCCAATATGATT               dehydrogenase       CCAC                       (SEQ ID NO: 562)               M539               CATTGACCTCAACTACATGGTTT   40 bp, e2-3, 35% GC                       ACATGTTCCAATATGAT                       (SEQ ID NO: 563)               M540               ACCTCAACTACATGGTTTACATGTTCC   30 bp, e2-3, 32% GC                       AAT                       (SEQ ID NO: 564)               M541               AGTATGACAACAGCCTCAAGATCATC   50 bp, e5-6, 50% GC                       AGCATGCCTCCTGCACCACCAAC                       (SEQ ID NO: 565)               M542               GACAACAGCCTCAAGATCATCAGCAA   40 bp, e5-6, 52% GC                       TGCCTCCTGCACCA                       (SEQ ID NO: 566)               M543               CAGCCTCAAGATCATCAGCAAT   30 bp, e5-6, 53% GC                       GCCTCCTG                       (SEQ ID NO: 567)               M544               AACTTTGGTATCGTGGAAGGACT   50 bp, e6-7, 50% GC                       CATGACGACAGTCCATGCCATC                       ACTGC                       (SEQ ID NO: 568)               M545   ACTB   actin, beta   NM_001101   AGATCACTGCCCTGGCACCCAG   50 bp, e4-5, 50% GC                       CAATGAAGATCAAGATCATTGC                       CT                       (SEQ ID NO: 569)               M546               GCCCTGGCACCCAGCACAATGA   40 bp, e4-5, 52% GC                       GATCAAGATCATTGCTC                       (SEQ ID NO: 570)               M547               TGGCACCCAGCACAATGAAGAT             30 bp, e2-3, 46% GC                       AAGATCA                       (SEQ ID NO: 571)               M548   Negative   Negative   NM_002046   ATGACAACAGCCTCAAGATCAT             50 bp, 44% GC           control   control   NM_001101   AGGAAGATCAAGATCATTGCTC             e5 of GAPD + e5                       CCT   ofACTB                       (SEQ ID NO: 572)               M549   Negative   Negative   NM_002046   AACAGCCTCAAGATCATCAGGA             40 bp, 42% GC           control   control   NM_001101   GATCAAGATCATTGCTC   e5 of GAPD + e5                       (SEQ ID NO: 573)   ofACTB               M550   Negative   Negative   NM_002046   CCTCAAGATCATCAGGAAGATC             30 bp, 40% GC           control   control   NM_001101   AGATCAT   e5 of GAPD + e5                       (SEQ ID NO: 574)   ofACTB               M551   PTGDS   prostaglandin   NM_000954   AACCTGACCTCCACCTTCCTCAG             50 bp, e2-3, 52% GC               D synthase       AAAAACCAGTGTGAGACCCGAA                                 CAT                       (SEQ ID NO: 575)               M552   PTGES   prostaglandin   NM_004878   ACGTGGAACGCTGCCTCAGGGC             42 bp, e2-3, 64% GC               E synthase       CACCGGAACGACATGGAGA                       (SEQ ID NO. 576)               M553   LYST   Chediak-Higas             U84744   CCTGTCCAAGAAGAAAAGGCAA             50 bp, e4-5, 46% GC               syndrome protein       AGATTTTAACCTACCGCTCTCAG                         short iso       AGA                       (SEQ ID NO: 577)               M554   MITF   microphthalmia             NM_000248   AATGCTAGAATATAATCACTAT             50 bp, e1-2, 44% GC               associated       GGTGCAGACCCACCTCGAAAAC                         transcription       CCA               factor       (SEQ ID NO: 578)               M555   OCA2   oculocutaneous   NM_000275   GTTTGCCTTTGTGGTGCTGTGTT             50 bp, e3-4, 44% GC               albinism II       ATTTTGTTCAGCCTATATCCGGA                                 C                       (SEQ ID NO: 579)               M556   OMP   olfactory marke             NM_006189   TGGCCAAGATCCGCAAGGTCAT             46 bp, 54% GC               protein       ACTTCCTCGTCACCTTTGGCGA                       (SEQ ID NO: 580)               M557   TRP-2   tyrosinase-   AJ000503   TCTTCCACCGGACCTGCAAGTG             50 bp, e1-2, 50% GC               related   NM_001922   CAGGAAACTTTGCCGGCTATAA                         protein 2       GT               (dopachrome       (SEQ ID NO: 581)               tautomerase)               M558               TATTCTGTTAGAGATACATTATT             50 bp, e2-3, 46% GC                       GGACCAGGACGCCCCTACAGGG                                 CAT                       (SEQ ID NO: 582)               M559   TYR   tyrosinase   NM_000372   AGCATCATTCTTCTCCTCTTGGC             50 bp, e1-2, 48% GC                       GATTGTCTGTAGCCGATTGGAG                                 GT                       (SEQ ID NO: 583)               M560   TYRP1   tyrosinase-   NM_000550   GCCTGTGACCAGAGGGTTCTCAT             50 bp, e2-3, 46% GC               related       GTCAGGAGAAATCTTCTGGACTT                         protein 1       AG                       (SEQ ID NO: 584)               M561   PNMT   phenylethanola             NM_002686   TGCCTCATTTGAGGGCAAGGGGG             41 bp, e2-3, 56% GC               ne N-methyl-       ATGCTGGCAGGATAAGGA               transferase       (SEQ ID NO: 585)               M562   HGF   hepatocyte   D14012   GCAGTGCACACAGGAAGTGGTG             50 bp, e4-5, 46% GC               growth       GCCACAACTCACAACTACGAC               factor       (SEQ ID NO: 586)               M563   MET   hepatocyte   NM_000245   GATCTGTCTGCCTGCAATCTACA             50 bp, e5-6, 46% GC               growth       GGTTTTCCCAAATAGTGCACCC                         factor       TG               receptor       (SEQ ID NO: 587)               M564   NPY   neuropeptide   NM_000905   ACATCAACCTCATCACCAGGCA             50 bp, e1-2, 50% GC               Y       AGATATGGAAAACGATCCAGCC                                 AGAG                       (SEQ ID NO: 588)               M565   NPY2R   neuropeptide   NM_000910   GCAAAAACGCGAGGTCCAGGT             49 bp, e1-2, 53% GC               Y receptor Y2       GTTGTAGACTCTTGTGCTGGTTG                                 AG                       (SEQ ID NO: 589)               M566       neuropeptide   M84755   ATTTATTTGCTACTTCAAGATAT             50 bp, e1-2, 28% GC               y receptor       TATACGCCTAAAAAGGAGAAA                                 ACA                       (SEQ ID NO: 590)               M567   BDNF   brain-derived   NM_001709   TGATAGAAGAGCTGTTGGATGA             50 bp, 44% GC               neurotrophic       GACCATAAAGTTCGGCCCAATG                         factor       AGAA                       (SEQ ID NO: 591)               M568   NTF3   neurotrophin 3   NM_002527   TTGCCAGAAGACTCGCTCAATT             50 bp, 46% GC                       CTCATTATTAAGCTGATCCAGG                                 GA                       (SEQ ID NO: 592)               M569   NTF5   neurotrophin 5   NM_006179   TCCGCCAGTACTTCTTTGAAAC             50 bp, e4-5, 46% GC                       GCTGCAAGGCTGATAACGGTGA                                 GAA                       (SEQ ID NO: 593)               M570   OGFR   opioid growth   NM_007346   TTGAGGAGAATCACTCCTACAT             50 bp, e4-5, 50% GC               factor receptor       AGTGGCTGTTTCCTCTGCGAGAA                                 CA                       (SEQ ID NO: 594)               M571   OPRD1   opioid receptor   NM_000911   CTTGTCATGTTCGGCATCGTCCG             50 bp, e1-2, 52% GC               delta 1       TACACTAAGATGAAGACGGCCA                                 CAA                       (SEQ ID NO: 595)               M572   OPRK1   opioid receptor   NM_000912   CGCTGGTCATGTTCGTGATCAT             50 bp, e1-2, 46% GC               kappa 1       GATACAGAAAGATGAAGACAG                                 ACC                       (SEQ ID NO: 596)               M573   OPRM1   opioid receptor   NM_000914   TCCTGGTCATGTATGTGATTGTC             50 bp, e1-2, 46% GC               mu 1       GATACACCAAGATGAAGACTGC                                 ACC                       (SEQ ID NO: 597)               M574   VIPR1   vasoactive   NM_004624   ATGACAAGGCAGCGAGTTTGGA             50 bp, e3-4, 50% GC               intestinal       GAGCAGCAGACCATGTITCTACG                         peptide       TCT               receptor 1       (SEQ ID NO: 598)               M575   VIPR2   vasoactive   XM_036413   CAGCGACCCGGAGGATGAGAG             50 bp, e4-5, 50% GC               intestinal       AGATCACGTTTTATATTCTGGTG                         peptide       AGG               receptor 2       (SEQ ID NO: 599)               M576   AKR1C2   aldo-keto   XM_048859   ATTTGGCACCTATGCGCCTGCA             50 bp, e1-2, 48% GC               reductase       GGTTCCTAAAAGTAAAGCTCTA             may also detect               family 1,       GG   AKR1C1, DD1               member C2       (SEQ ID NO: 600)               M577   AKR1C3   aldo-keto   NM_003739   TCATTCTCCAATGTCTCTAAAG             50 bp, e3-4, 44% GC               reductase       AGGTGAGGAACTTTCACCAACA                         family 1,       ATG               member C3       (SEQ ID NO: 601)               M578   CYP8B1   cytochrome P4             XM_046266   ATGTTTGAATTTCTGAAGCGCAT             50 bp, 46% GC               subfamily VIIII                 AGGACCAAGGATGGGGATGTGT                                 GAG                       (SEQ ID NO: 602)               M579   EBRP   emopamil   NM_032565   CCATAGTCAAAGAAAAATATTA             50 bp, e3-4, 44% GC               binding       CGGCATTTCCTGCAGATCACCCT                         related       TGC               protein       (SEQ ID NO: 603)               M580   HSD3A   oxidative 3   U89281   GGAAGCCTTTTCAGATATTCTGA             50 bp, e1-2, 44% GC               alpha hydroxy-       GCGTGAGATTCAACATTTTGGG                         steroid       GA               dehydrogenase       (SEQ ID NO: 604)               M581   HSD3B   3 Beta-   X53321   TCTATCATGAATGTCAATGTGAA             50 bp, e2-3, 46% GC               hydroxysteroid       GGTACCCAGCTCCTGTTAGAGG                         dehydrogenase       TG                       (SEQ ID NO: 605)               M582   RDHL   retinol   NM_005771   GTGAAGAACCAAGTTGGGGAGA             50 bp, e1-2, 48% GC               dehydrogenase       AGGTCTCTGGGGTCTGATCAATA             will also detect               homolog       TGC   hydroxysteroid                       (SEQ ID NO: 606)   epimerase                           (NM_003725)               M583       sterol/retinol   AF057034   TGAAGGAGTGCGTGAGAGACAA             50 bp, e1-2; 52% GC               dehydrogenase       GGACTCTGGGGGCTGGTGAATA                                 GCT                       (SEQ ID NO: 607)               M584   TAC1   tachykinin,   NM_003182   GGATTAATGGGCAAACGGGATG             50 bp, e3-4, 44% GC               precursor 1       TGATTCCTCAATTGAAAAACAA                                 GGC                       (SEQ ID NO: 608)               M585   TACR1   tachykinin   NM_001058   GAGCAAGTCTCTGCCAAGCGCA             50 bp, e3-4, 50% GC               receptor 1       GGTGGTCAAAATGATGATTGTC             will detect both                       GGT   isoforms                       (SEQ ID NO: 609)               M586   TACR2   tachykinin   NM_001057   ATCTACTGCTGTCTCAACCACA             50 bp, e4-5, 54% GC               receptor 2       TTTCGCTCTGGGTTCCGGCTTGC                                 TT                       (SEQ ID NO: 610)               M587   TACR3   Tachykinin   NM_001059   TCATCTACTGCTGTCTGAATAAA             50 bp, e4-5, 42% GC               receptor 3       GATTTCGAGCTGGCTTCAAGAGA                                 CA                       (SEQ ID NO: 611)               M588   TBG   thyroxine-   M14091   GAGGACAATGGTCTGAAACTTT             50 bp, e4-5, 50% GC =               binding       AATGCTGCCCATAAGGCTGTGCT             serine (or cysteine)               globulin       CA   proteinase inhibitor                       (SEQ ID NO: 612)               M589   TPO   thyroid   NM_000547   CCGCCATGTACGCCACGATGCA             50 bp, e2-3, 50% GC               peroxidase       AGAAACCTCAAGAAAAGAGGAA                                 CCTT                       (SEQ ID NO: 613)               M590   CALB2b   calbindin 2   NM_007087   GCTCCAGGAATACACCCAAACC             50 bp, e5-6, 46% GC                       TACTACGGATGTTTGACTTGAA                                 GGG                       (SEQ ID NO: 614)                   M591   CALB3   calbindin 3   NM_004057   GCTGAATTCCCCAGTTTACTCAA             50 bp, e2-3, 44% GC                       GGTCCAAACACCCTAGATGATCT                                 TT                       (SEQ ID NO: 615)               M592   NCOA2   nuclear   NM_006540   AAAAACCTGCTGCCAAAGTCTAGG             50 bp, e6-7, 46% GC               receptor       GTAAATGGGGGATCTTGGTCTGGAA                         coactivator 2       GA                       (SEQ ID NO: 616)               M593   VDR   vitamin D   NM_000376   GTGGACATCGGCATGATGAAGG             50 bp, e3-4, 48% GC               receptor       GTTCATTCTGACAGATGAGGAA                                 GCA                       (SEQ ID NO: 617)               M594   VDBP   vitamin D   NM_000583   CTCTGAAGATTGCATGGCCAAA             50 bp, e7-8, 46% GC               binding       AGCTGCCTGAACACACAGTAAA                         protein       CTCT                       (SEQ ID NO: 618)               M595   BMP1   bone   NM_006128   TCGTAAGTCCTCCATCAAAGCT             50 bp, e2-3, 50% GC               morphogenetic       AGTTCCAGGAAACACTTCTACC                         protein 1       CA                       (SEQ ID NO: 619)               M596   BMP10   bone   NM_014482   CATCATTAGGAGTTTCAAGAAT             50 bp, e1-2, 42% GC               morphogenetic       AGATCTGTTTTCCCAGCCGGTCA             detects almost all               protein 10       TT   splicing variants                       (SEQ ID NO: 620)               M597   BMP11   bone   AF100907   GTCATTAGCATGGCCCAGGAGA             45 bp, e1-2, 55% GC               morphogenetic       GGACCCAGCAGTACAGACAGAT                         protein 11       (SEQ ID NO: 621)               M598   BMP15   bone   NM_005448   ACCAGTGTGGCAAGGCCTCACA             47 bp, e1-2, 53% GC               morphogenetic       AGGTACCTGGCATATACAGATC                         protein 15       (SEQ ID NO: 622)               M599   BMP2   bone   NM_00120   ACTGTGCGCAGCTTCCACCATGA             50 bp, e2-3, 46% GC               morphogenetic       GAATCTTTGGAAGAACTACCAG                         protein 2       AAC                       (SEQ ID NO: 623)               M600   BMP3   bone   NM_001201   CAGCTTTCGGGCGGCAGCAGCA             50 bp, e1-2, 48% GC               morphogenetic       AAACTCTTGAAAGAAAAGGACT                         protein 3       TATA                       (SEQ ID NO: 624)               M601   BMP4   bone   NM_001202   AGCTTCCACCACGAAGAACATC             50 bp, e3-4, 50% GC               morphogenetic       GAGAACATCCCAGGGACCAGTG                         protein 4       AAA                       (SEQ ID NO: 625)               M602   BMP5   Bone   NM_021073   CAGCTCTGTGCAGAAACAGGGG             50 bp, e1-2, 48% GC               morphogenetic       TGGACGCAGTATCAACGTAAAA                         protein 5       GTGC                       (SEQ ID NO: 626)               M603   BMP6   bone   NM_001718   ACATGGTCATGAGCTTTGTGAA             50 bp, e1-2, 50% GC               morphogenetic       TGGTGGAGTACGACAAGGAGTT                         protein 6       TCC                       (SEQ ID NO: 627)               M604   BMP7   bone   NM_001719   ATGGTCATGAGCTTGGTCAACCT             50 bp, e1-2, 46% GC =               morphogenetic       GTGGAACATGACAAGGAATTCT             TGF beta?               protein 7       CA                       (SEQ ID NO: 628)               M605   BMP8   bone   NM_001720   AGGTGGTCCAGGAGCAGTCCAA             50 bp, e3-4, 50% GC               morphogenetic       AGGGAGTCTGACTTGTTCTTTTT                         protein 8       GAT                       (SEQ ID NO: 629)               M606   BMP9   bone   AF188285   ATTGTGCGGAGCTFCAGCATGGA             50 bp, e1-2, 50% GC               morphogenetic       GATGCCATCTCCATAACTGCCA                         protein 9       GA                       (SEQ ID NO: 630)               M607   BMPR1A   bone   NM_004329   AGTGGGTCTGGACTACCTTTATT             50 bp, e8-9, 44% GC               morphogenetic       GTTCAGCGAACTATTGCCAAACA                         protein       AT               receptor       (SEQ ID NO: 631)               type IA               M608   BMPR1B   bone   NM_001203   GAAGGCTCAGATTTTCAGTGTC             50 bp, e5-6, 48% GC               morphogenetic       GACACTCCCATTCCTCATCAAA                         protein       AG               receptor       (SEQ ID NO: 632)               type IB               M609   BMPR2   bone   NM_001204   TGCTTTGGATACAGAATGTTGA             50 bp, e4-5, 44% GC               morphogenetic       GGAGACCGTAAACAAGGTCTTC                         protein       CAG               receptor       (SEQ ID NO: 633)               type 2               M610   GDF1   growth   NM_001492   TCTTCTTTGACAACAGCGACAA             49 bp, e2, 51% GC               differentiation       TGGTGCTGCGGCAGTATGAGGA                         factor 1       AT                       (SEQ ID NO: 634)               M611   GDF1   growth   NM_004962   CATGTGAGTTCCCCATGCCTAA             48 bp, e3-4, 52% GC               differentiation       TCGTTCGTCCATCCAACCATGC                         factor 10       (SEQ ID NO: 635)               M612   GDF3   Growth   NM_020634   GGAATGTACTTCGCTTTCTCCCA             50 bp, e1-2, 48% GC               differentiation       ACCAAGGTTTCTTTCTTTACCCA                         factor 3       AG                       (SEQ ID NO: 636)               M613   GDF7   Growth   NM_000557   ACCAGCTTTATTGACAAAGGGC             50 bp, e1-2, 52% GC               differentiation       AGATGACCGAGGTCCCGTGGTC                         factor 7       GGAA                       (SEQ ID NO: 637)               M614   GDF8   Growth   NM_005259   CAGGACCAGGAGAAGATGGGCT             50 bp, e2-3, 48% GC               differentiation       AATCCGTTTTTAGAGGTCAAGGT                         factor 8       ACA                       (SEQ ID NO: 638)               M615   GDF9   Growth   NM_005260   AAGCAGGCTCCTGGAGACCAGG             48 bp, e1-2, 52% GC               differentiation       AACAGGAATCCTTCCATCAGTG                         factor 9       A                       (SEQ ID NO: 639)               M616   AQP1   aquaporin 1   NM_000385   TGTAGCCCTTGGACACCTCCTG             50 bp, e2-3, 52% GC                       TATTGACTACACTGGCTGTGGGA                                 TA                       (SEQ ID NO: 640)               M617   AQP10   aquaporin 10   NM_080429   GGAGCCACCTATGTTCTCTACCA             50 bp, e3-4, 50% GC                       GATGCCCTACAGAACTATACAG                                 GG                       (SEQ ID NO: 641)               M618   AQP2   aquaporin 2   NM_000486   TGTCGTCACTGGCAAATTTGAT             48 bp, e3-4, 52% GC                       CCACTGGGTCTTCTGGATCGGA                                 (SEQ ID NO:642)               M619   AQP3   aquaporin 3   NM_004925   CTGGAATAGTTTTTGGGCTGTAT             50 bp, e3-4, 44% GC                       ATGATGCAATCTGGCACTTTGC                                 AC                       (SEQ ID NO: 643)               M620   AQP4   aquaporin 4   NM_001650   CAGTTATCATGGGAAATTGGGA             50 bp, e4-5, 48% GC                       AACCAUGGATATATTGGGTTG                                 CCC                       (SEQ ID NO: 644)               M621   AQP5   aquaporin 5   NM_001651   ACCCTGGGCCACCTTGTCGGAAT             47 bp, e4-5, 55% GC                       TACTTCACTGGCTGCTCCATGAA                                 (SEQ ID NO: 645)               M622   AQP6   aquaporin 6   XM_113241   ATCCGAGAGACCCTTGGGATCA             48 bp, e1-2, 54% GC                       CGTGGTCCGGAACAGTGTCTCAA                                 T                       (SEQ ID NO: 646)               M623   AQP7   aquaporin 7   NM_001170   AGTTCATGAGCACATATGTCAT             50 bp, e3-4, 50% GC                       TGGTATTCGGCCTTGGTTCCGTT                                 CC                       (SEQ ID NO: 647)               M624   AQP8   aquaporin 8   NM_001169   GCCATCTGATCCTGATGTCTGGA             50 bp, e1-2, 48% GC                       AGATAGCCATGTGTGAGCCTGA                                 TT                       (SEQ ID NO: 648)               M625   AQP9   aquaporin 9   NM_020980   CGTTCATCTTGATTGTCCTTGGA             50 bp, e1-2, 48% GC                       GTGGCTGTGTTGCCCAAGCTATT                                 TC                       (SEQ ID NO: 649)               M626   SLC6A1   neurotransmitter   NM_003042   TACCTGTACAACTCCTTCACCA             50 bp, e4-5, 52% GC               transporter,       ACACTGCCGTGGAAACAGTGCG                         GABA       CAA                       (SEQ ID NO: 650)               M627   SLC6A11   neurotransmitter   NM_014229   GGAGGAAAGTTTGCCCTTTATTT             50 bp, e2-3, 46% GC               transporter,       AAGGCATTGGCTATGCAACACA                         GABA       GTG                       (SEQ ID NO: 651)               M628   SLC6A9   neurotransmitter       TGGAGGATCAGCCCCATGTTCAA             50 bp, e3-4, 52% GC               transporter 9       GGAGTGGGCTATGGTATGATGG                                 GGT                       (SEQ ID NO: 652)               M629   SLC6A13   neurotransmitter   NM_016615   TATCTCTGCTACAAAAATGGGG             50 bp, e1-2, 50% GC               transporter,       GGTGCCTTCTTCATCCCCTACCT             may detect also               GABA       GT   SLC6A12                       (SEQ ID NO: 653)               M630   SLC6A14   neurotransmitter   NM_007231   GTTTCTTCAAGTGCAGGGAGAA             50 bp, e1-2, 46% GC               transporter       GAGAAAGTGTCGGCTTCATCAG                                 GAAT                       (SEQ ID NO: 654)               M631   SLC6A2   neurotransmitter   NM_001043   TGGAAAATCTGCCCATTCTTCAA             50 bp, e2-3, 48% GC               transporter,       GGCGTTGGCTATGCTGTCATCCT                         noradrenalin       AT                       (SEQ ID NO: 655)               M632   SLC6A3   neurotransmitter   NM_001044   AACAAGTTCACCAACAACTGCTA             50 bp, e7-8, 50% GC               transporter,       AGGGACGCGATTGTCACCACCT                         dotamine       AT                       (SEQ ID NO: 656)               M633   SLC6A4   neurotransmitter   NM_001045   ACAACAAGTTCAACAACAACTG             50 bp, e6-7, 50% GC               transporter,       TACCAAGATGCCCTGGTGACCA                         serotonin       GTG                       (SEQ ID NO: 657)               M634   SLC6A5   neurotransmitter   NM_004211   AGACTTCAGGAAAAGTGGTGTA             50 bp, e7-8, 50% GC               transporter,       TTCACGGCCACGTTCCCGTATGT                         glycine       GTA                       (SEQ ID NO: 658)               M635   SLC6A6   neurotransmitter   NM_003043   ACAAGTACAAGTATAACTCGTA             50 bp, e3-4, 48% GC               transporter,       AGGGACTGTATGCTGCTGGGAT                         taurine       CTG                       (SEQ ID NO: 659)               M636   SLC6A7   neurotransmitter   NM_014228   CCACCACTTGTTGTCTTCCAAGG             50 bp, e6-7, 48% GC               transporter, L-       GTGGATTGAAGCTGCTCTTCAGA                         proline       CT                       (SEQ ID NO: 660)               M637   SLC6A8   neurotransmitter   NM_005629   CGCTTCAACAACAACTGCTACAA             50 bp, e6-7, 50% GC               transporter,       GACGCCATCATCCTGGCTCTCAT                         creatine       AA                       (SEQ ID NO: 661)               M638   GABBR1   gamma-   NM_001470   CAATACCCGCAGCATTTCCAACA             50 bp, e9-10, 46% GC               aminobutyric a                 GACATCCCAGGAATTTGTGGAG                         B receptor, 1       AAC                       (SEQ ID NO: 662)               M639   GABRA1   gamma-   NM_000806   TTCTGAGCACACTGACTGGAAGA             50 bp, e2-3, 46% GC               aminobutyric a                 AGCTATGGACAGCCGTCATTACA                         A receptor,       GAT               alpha 1       (SEQ ID NO: 663)               M640   GABRA2   gamma-   NM_000807   GATAATCGGCTTAGACCAGGAC             50 bp, e3-4, 46% GC               aminobutyric a                 GGGAGACAGTATTACTGAAGTC                         A receptor,       GAG               alpha 2       (SEQ ID NO: 664)               M641   GABRA3   gamma-   NM_000808   CTGCGACCTGGGCTTGGAGATG             50 bp, e3-4, 52% GC               aminobutyric a                 GTGACTGAAGTGAAGACTGACA                         A receptor,       CTA               alpha 3       (SEQ ID NO: 665)               M642   GABRA4   gamma-   NM_000809   AGGCTGCGTCCTGGATTTGGGG             50 bp, e2-3, 50% GC               aminobutyric a                 CCTGTTACAGAAGTGAAAACTG                         A receptor,       CAT               alpha 4       (SEQ ID NO: 666)               M643   GABRA5   gamma-   NM_000810   ACTGAGAACATCAGCACCAGCA             50 bp, e1-2, 48% GC               aminobutyric a                 AGGCGAATACACAATCATGACA                         A receptor,       CTCA               alpha 5       (SEQ ID NO: 667)               M644   GABRA6   gamma-   NM_000811   ATGGAACCATTTTATACACCAT             50 bp, e4-5, 46% GC               aminobutyric a                 GGCTTACCATCAATGGTGACTGT                         A receptor,       CC               alpha 6       (SEQ ID NO: 668)               M645   GABRB1   gamma-   NM_000812   TGATGGAACAGTTCTCTATGGA             50 bp, e4-5, 46% GC               aminobutyric a                 CCGAATCACAACCACAGCTGCA                         A receptor,       GTA               beta       (SEQ ID NO: 669)               M646   GABRB2   gamma-   XM_011222   ACCAAGAAGGTTGTTTTTTCCA             50 bp, e1-2, 48% GC               aminobutyric a                 GGTTCCTATCCCAGGTTATCCCT                         A receptor,       AG               beta       (SEQ ID NO: 670)               M647   GABRB3   gamma-   XM_007751   GAACTGCACTCTGGAAATTGAA             50 bp, e4-5, 46% GC               aminobutyric a                 GCTATGGCTACACCACGGATGA                         A receptor,       ATTG               beta       (SEQ ID NO: 671)               M648   GABRD   gamma-   NM_000815   AGCATCGACCACATCTCAGAGG             50 bp, e2-3, 52% GC               aminobutyric a                 CAACATGGAGTACACCATGACG                         A receptor,       TGTT               delta       (SEQ ID NO: 672)               M649   GABRE   gamma-   NM_004961   GGATGGCAAGGTGTTGTACACA             50 bp, e4-5, 48% GC               aminobutyric a                 TTAGGATGACCATTGATGCCGGA                         A receptor,       GCT               epsilon       (SEQ ID NO: 673)               M650   GABRG2   gamma-   NM_000816   TGGTCGAGTGCTCTACTCCCTAA             50 bp, e4-5, 48% GC               aminobutyric a                 GTTGACAATTGATGCTGAGTGC                         A receptor,       AT               gamma 2       (SEQ ID NO: 674)               M651   GABRG3   gamma-   NM_033223   AAAAGATGCTACGCCAGCAAGA             50 bp, e6-7, 50% GC               aminobutyric a                 CAGCATTAGGCATCACCACGGT                         A receptor,       TGA               gamma 3       (SEQ ID NO: 675)               M652   GABRP   gamma-   XM_003708   AATTTCTCAGGCCCAATTTTGGT             50 bp, e3-4, 48% GC               aminobutyric a                 GAGAACCCGTACAGATAGCGCT                         A receptor, pi       ACT                       (SEQ ID NO: 676)               M653   GABRR1   gamma-   NM_002042   ATGAAGATGCCCACAAGCAAGT             50 bp, e2-3, 50% GC               aminobutyric a                 AGCCCAATTCTGAGACGAAGTC                         A receptor, rho       GAC                       (SEQ ID NO: 677)               M654   GABR2   gamma-   NM_002043   CAGACCTGTTCTTTGGAGCTGGA             50 bp, e1-2, 48% GC               aminobutyric a                 AGCTATGCCTATACAGATGAAG                         A receptor, rho       CT                       (SEQ ID NO: 678)                   M655   CRH-R1           GGGCCCCATGATCCTGGTCCTG             50 bp, e10-11, 48% G                                 GATCAATTTCATCTTCCTTTTCAA                                 A                       (SEQ ID NO: 679)               M656               CCATGATCCTGGTCCTGCTGAT             40 bp, e1-2, 42% GC                       ATTTCATCTTCCTTTT                       (SEQ ID NO: 680)               M657               ATCCTGGTCCTGCTGATCAATTT             30 bp, e1-2, 42% GC                       ATCTTC                       (SEQ ID NO: 681)               M658       Insulin       AGCTGGAGAACTACTGCAACTA             40 bp                       ACGCAGCCCGCAGGCAG                       (SEQ ID NO: 682)               M659       Insulin       CACCACTTCTTCTGACATGTGGT             45 bp               receptor       CTTTGGCGTGGTCCTTTGGGA                       (SEQ ID NO: 683)               M660       Insulin       TGTCACAAGCACCAGCCTTGCCA             45 bp               degradi                 AACCTGAAGTGATTCAGAACA               enzyme       (SEQ ID NO: 684)               M661       Proenkephalin       GCCGACATCAACTTCCTGGCTT             45 bp                       GTAATGGAATGTGAAGGTAAA                       (SEQ ID NO: 685)               M662       Pituitary       GGATCAGGCCAGAGGAAGAGG             40 bp               adenylate cycla                 TACGGCGAGGACGGAAA               activating       (SEQ ID NO: 686)               peptide               M663       Pituitary       GCAACCACTGCTTCATCTCCACT             45 bp Detects recepto                         adenylate cycla                 TGGAATGTAAGGCCGTCATGG   A, B, B2, C               activating       (SEQ ID NO: 687)               peptide               recetor A               M664   PC1   PC1 (NEC1   M90753   AGAAGATGGTGGATCCAGGGGA             45 bp                       GAGCAGCCCACACAAGAGAAC                                 (SEQ ID NO: 688)               M665   PC2   proprotein   NM_002594   AGGAAAAGGAACCCCGAGGCC                         convertase       TGTGGCAACCACAGATTTGTAC               subtilisin/kexin       (SEQ ID NO: 689)               type 2               M666       polypeptide 7B   Y00757   GAAGGGAGGAGAGAGACGAAA                                 CGGAGGAGTGTCAATCCATATC                                 (SEQ ID NO: 690)               M667   COL14A   collagen, type   XM_044622   CAGTGTTTATACAAAGCTCCAG             50 bp, 42% GC               XIV, alpha 1       GATTGAAGGACCTAGTGTGAGC                                 TAA                       (SEQ ID NO: 691)               M668   COL1A1   collagen, type   NM_000088   ATGCCATCAAAGTCTTCTGCAA             50 bp, 50% GC               I alpha 1       TGGAGACTGGTGAGACCTGCGT                                 AC                       (SEQ ID NO: 692)               M669   COL1A2   collagen, type   NM_000089   TGAACTTGTTGCTGAGGGCAACA             50 bp, 46% GC               I alpha 2       CAGGTTCACTTACACTGTTCTTG                                 AG                       (SEQ ID NO: 693)               M670   COL3A1   collagen, type   NM_000090   GTAGATATTGCACCCTATGACAT             50 bp, 42% GC; dete                         I alpha 1       GGTGGTCCTGATCAAGAATTTG             also COL5A2                       GT                       (SEQ ID NO: 694)               M671   COL5A1   collagen, type              NM_000093   GGACATCATGTTCAATGACTTC             50 bp, 42% GC               alpha 1       TGAAGCGTCACAGAAATTTGGA                                 TG                       (SEQ ID NO: 695)               M672   COL7A1   collagen, type   NM_000094   AGTATTCTGTGGAGGAGTACCA             50 bp, 50% GC               VII, alpha 1       GACCCTGAAGCTCCTTGGGATA                                 GAT                       (SEQ ID NO: 696)               M673   FN1   fibronectin 1   NM_002026   TGAGTGCTTCATGCCTTTAGATG                                 ACAGGCTGACAGAGAAGATTCC                                 GAG                       (SEQ ID NO: 697)               M674   LAMA2   laminin,   NM_000426   ATCAGATCCCTGAAGCTCACCAA                         alpha 2       GGCACAGCAAGCCACTGGAGGT                         (merosin)       AAT                       (SEQ ID NO: 698)               M675   LAMB2   laminin,   NM_002292   GACCAGCATCTTGACTTGCTCAA                         beta 2       CATTCAAACTTCCTGGGTGCCTA                                 GA                       (SEQ ID NO: 699)               M676   LAMB3   laminin,   NM_000228   GATTGGGTTGGAATGCTTTCCAT                         beta 3       TCCAGGAGACTTTCATGCAGCCT                                 AA                       (SEQ ID NO: 700)               M677   LAMC2   laminin,   NM_018891   TTACAATCCAAGACACACTCAA                         gamma       CATTAGACGGCCTCCTGCATCT                                 TG                       (SEQ ID NO: 701)               M678   LOX   lysyl   NM_002317   GACTATACCAACAATGTTGTGC                         oxidase       TGTGACATTCGCTACACAGGACA                                 CA                       (SEQ ID NO: 702)               M679   MMP1   interstitial   NM_002421   ATCTACAGCTCCTTTGGCTTCCC                         collagenase       AGAACTGTGAAGCATATCGATG                                 GC                       (SEQ ID NO: 703)               M680   MMP10   stromelysin 2   NM_002425   TCAGTGGATCATCACAGTTTGA                                 TTGACCCCAATGCCAGGATGGT                                 CA                       (SEQ ID NO: 704)               M681   MMP11   stromelysin 3   NM_005940   CCTCTACTGGAAGTTTGACCCT                                 GAAGGTGAAGGCTCTGGAAGG                                 T                       (SEQ ID NO: 705)               M682   MMP3   stromelysin 1   NM_002422   AGGAAATCAATTCTGGGCCATC                                 GAGGAAATGAGGTACGAGCTG                                 TACC                       (SEQ ID NO: 706)               M683   MMP9   gelatinase B   NM_004994   AGTGAGTTGAACCAGGTGGACC                                 AGTGGGCTACGTGACCTATGAC                                 (SEQ ID NO: 707)               M684   PLOD   lysine   NM_000302   AGAACGTGCCGACTATTGACAT                         hydroxylase       ACATGAACCAGATCGGCTTTGA                                 (SEQ ID NO: 708)               M685       prolyl 4-   X05130   AGGAGCCAGACATGGAGGAAGA                         hydoxylase       GATGACCAGAAAGCTGTGAAAG                         beta subunit       TGAA                       (SEQ ID NO: 709)               M686       prolyl 4-   U12762   GTCTTCACTGAGGCCAAGTCTA                         hydroxylase       ATTCTGCCCACCAAGAATGATG                         alpha       CT               subunit       (SEQ ID NO: 710)               M687   TNXB   tenascin XB   NM_019105   ACTATGCCCATGGTTTTGGGAA                                 TCTCTGGAGAGTTCTGGCTGGG                                 AT                       (SEQ ID NO: 711)               M688   VTN   vitronectin   NM_000638   CTTCTTCTTCTCTGGAGACAAGT                                 CTACCGAGTCAATCTTCGCACA                                 GC                       (SEQ ID NO: 712)               M689   FLG   filaggrin   XM_048104   ATCATCCCATGAACAGGCAAGA                                 CAAGTGCAGGAGAGAGACATG                                 TCCC                       (SEQ ID NO: 713)               M690   IVL   involucrin   NM_005547   AGAGCAGCAGGTAGGACAGCCA                                 AGCACCTGGAACAGCAGGAAA                                 CA                       (SEQ ID NO: 714)               M691   KRT1   keratin 1   NM_006121   CAGTTCCAGCGTGAGGTTTGTTT                                 TACCACTTATTCCGGAGTAACCA                                 AT                       (SEQ ID NO: 715)               M692   KRT10   keratin 10   NM_000421   CTGAAACCGAGTGCCAGAATAC                                 GAATACCAACAACTCACGGATA                                 AAG                       (SEQ ID NO: 716)               M693   KRT14   keratin 14   NM_000526   TGACCAGTATGAGAAGATGGCA                                 AGAAGAACCGCAAGGATGCCGA                                 GAAT                       (SEQ ID NO: 717)               M694   KRT17   keratin 17   NM_000422   GAGATGCGTGACCAGTATGAGA                                 GATGGCAGAGAAGAACCGCAA                                 ATG                       (SEQ ID NO: 718)               M695   KRT19   keratin 19   NM_002276   TGCTCGAGGGACAGGAAGATCA                                 TACAACAATTTGTCTGCCTCCAA                                 GTC                       (SEQ ID NO: 719)               M696   KRT5   keratin 5   NM_000424   GAAGAGCTTCAAGAGCTAAGAA                                 CTGCTGCAAGTCACTGCCTTCCA                                 GTG                       (SEQ ID NO: 720)               M697   KRTHA5   keratin,   NM_002280   TGGAGATTGAGCTGCAGGCTCA                         hair,       CACAGCATGAGAGATGCTTTGG                         acidic, 5       AT                       (SEQ ID NO: 721)               M698   KRTHB5   keratin,   NM_002283   ATCACTGCACTGAATGGCATGT                         hair,       ATGGAAAATGTGTGCTTGCTTC                         basic, 5       GA                       (SEQ ID NO: 722)               M699   LOR   loricrin   NM_000427   TGCAAATCCTTCATGTCTTAAC                                 ACCTGGAAGAAGCCATTGAGCT                                 CC                       (SEQ ID NO: 723)               M700   TGMI   transgluta-   NM_000359   ACAGTGAAACTGCACCTCTACCT                         minase 1       TCAGTCACTTTCTATACTGGTGT                                 AG                       (SEQ ID NO: 724)               M701   TGM2   transgluta-   NM_004613   TCCTCTTTGCATCCTCTATGAGA                         minase 2       ATACCGTGACTGCCTTACGGAGT                                 CA                       (SEQ ID NO: 725)               M702   TGM3   transgluta-   NM_003245   AAGAGGCAGAACATCCCATAAA                         minase 3       ATCTCGTACGCTCAGTATGAGA                                 TAG                       (SEQ ID NO: 726)               M703   THH   trichohyalin   NM_007113   CTTAAGTGATGTTGCCAATATCT                                 GACACCTGCCAAAGCTTCCAGCA                                 GG                       (SEQ ID NO: 727)               M704   TRHY   trichohyalin   L09190   CAATACCGCCCTTAAGTGATGTT                                 CCAATATCTTGACACCTGCCAAA                                 CT                       (SEQ ID NO: 728)               M705       agouti   U12770   TATATAGGCTGCTCGAAGGTGT                                 GGCTGTTTCTGTAAAGGTCCCGA                                 AG                       (SEQ ID NO: 729)               M706   AGRP   agouti   NM_001138   TTCTTCAATGCCTTCTGCTACTG                         related       GCAAGCTGGGTACTGCCATGAA                         protein       (SEQ ID NO: 730)               M707   CCS   copper   NM_005125   CAGAATGGAGGATGAGCAGCTG                         chapero                 AGGTGTGGGATGTGATTGG               for super-       (SEQ ID NO: 731)               oxide               dismutase               M708   GCH1   GTP   NM_000161   GCTACCAGGAGACCATCTCAGA                         cyclo-       GTCCTAAACGATGCTATATTTGA                         hydrolase       GAA                       (SEQ ID NO: 732)               M709   GGT1   gamma-   NM_005265   ACAACCAGCTTCTGCCCAACGT                         glutamyl-       CGACAGTGGAGAGAAACATTGA                         transferase 1       CA                       (SEQ ID NO: 733)               M710   GSR   glutathione   NM_000637   CAGTGAAGATGGGAGCAACGAA                         reductase       GCAGACTTTGACAACACAGTCG                                 ATT                       (SEQ ID NO: 734)               M711   LAMP1   lysosomal-   NM_005561   CAAGGAATCCAGTTGAATACAA                         associated       CTTCCTGACGCCAGAGACCCTG                         membrane       TT               protein 1       (SEQ ID NO: 735)               M712   Pmel17       S73003   GCAGGTCTGAGTACTCTCATAT                                 TGCTGTGATTTTCCTGGAGTTGA                                 AG                       (SEQ ID NO: 736)               M713   TXNRD1   thioredoxin   NM_003330   TCAGAGGCTCTATGCAGGTTCCA                         reductase 1       TGTCAAGTGTGACTATGAAAAT                                 TC                       (SEQ ID NO: 737)               M714   TXNRD2   thioredoxin   NM_006440   GCGAAGTTACTCAAGGATTTGCT                         reductase 2       TGGGGATCAAGTGTGGGGCTTC                                 AT                       (SEQ ID NO: 738)               M715   BMAL1a       D89722   CAACGACCAAGGATCAAGTAGT                                 CCAGTAATGATGAGGCAGCAAT                                 GCTG                       (SEQ ID NO: 739)               M716   CLOCK   clock   NM_+ AF8-   ACAACAGCAACAGTCACAGACA                         homolog   004898   TGTCAGTAACGCAGCAGCAGCA                                 CAG                       (SEQ ID NO: 740)               M717   CRY1   cryptochrome   NM_004075   CTATCAGCAGCTTTCACGATATA                         1       AGGACTAGGTCTTCTGGCATCA                                 AC                       (SEQ ID NO: 741)               M718   CRY2   cryptochrome   XM_051030   TATGGCGAGAGTTCTTCTACAC                         2       CAGCTACCAACAACCCCAGGTTT                                 A                       (SEQ ID NO: 742)               M719   CYP39A1   cytochrome P4             NM_016593   TGAATATAAACAAAGAATATGA                         subfamily       ATCTGTTGGGCCTCACAAGGAC                         XXXIX       GGG               (oxysterol 7       (SEQ ID NO: 743)               alpha-               hydroxylase),               polypeptide 1               M720   CYP7A1   cholesterol   NM_000780   ATCCGGACAGCTAAGGAGGATT                         7alpha-       CACTTTGCACCTTGAGGACGGTT                         monooxygenase       CTA                       (SEQ ID NO: 744)               M721   CYP7B1   oxysterol 7   NM_004820   CCTACATGGTGACCCTGAAATCT                         alpha       TGAAGCTCCAGAGGAGTTTAGA                         hydroxylase       ATG                       (SEQ ID NO: 745)               M722   DHCR7   7-dehydro-   NM_001360   TGCAGGCCATCTACGTGATTGA                         cholesterol       TCTTCTGGAACGAAACCTGGTA                         reductase       TG                       (SEQ ID NO: 746)               M723   PAH   phenylalanine   NM_000277   GCCTCTCTGGGTGCACCTGATGA                         hydroxylase       TACATTGAAAAGCTCGCCACAA                                 TA                       (SEQ ID NO: 747)               M724   PER1   period   NM_002616   CAGTCCAGCCTTACCTACAGCA                         homolog       AAACTGCACCAGCTAGACTCCA                                 CT                       (SEQ ID NO: 748)               M725   PER2   period   NM_022817   CCTTCTCTGGGACTCAGCGAAGT                         homolog       TCGGACACCAAAGAAGACGAAA                                 TGG                       (SEQ ID NO: 749)               M726   PER3   period   NM_+ AF8-   CTCATGGGCAAAAGGAGGAGCT                         homolog   016831   GCTAAGGTGTATAATTGGATTCA                                 AGC                       (SEQ ID NO: 750)               M727   TH   tyrosine   NM_000360   CTACCAAGACCAGACGTACCAG                         hydroxylase       CAGTCTACTTCGTGTCTGAGAG                                 TCA                       (SEQ ID NO: 751)               M728   GSTA1   glutathione   NM_145740   ACCAGAGCCATTCTCAACTACAT                         S-transferase       GCCAGCAAATACAACCTCTATG                         A1       GAA                       (SEQ ID NO: 752)               M729   GSTA2   glutathione   NM_000846   TGAAAACCAGAATCAGTAACCT                         S-transferase       CCCACAGTGAAGAAGTTTCTACA                         A2       CCT                       (SEQ ID NO: 753)               M730   GSTA3   glutathione   NM_000847   CATTGCCAGCAAATACAACCTCT                         S-transferase       CGGGAAAGACATAAAGGAGAGA                         A3       CCC                       (SEQ ID NO: 754)               M731   GSTA4   glutathione   NM_001512   CAGACCCGAAGCATTCTCCACTA                         S-transferase       ATAGCAGACAAGCACAATCTCT                         A4       GG                       (SEQ ID NO: 755)               M732   GSTM1   glutathione   NM_000561   AGTACTTGGAGGAACTCCCTGAA                         S-transferase       AAGCTAAAGCTCTACTCAGAGTT                         M1       CTG                       (SEQ ID NO: 756)               M733   GSTM3   glutathione   NM_000849   AGCTCTGACCACGAAAAACTGA                         S-transferase       GCCTCAGTACTTGGAAGAGCTA                         M3 (brain)       TGG                       (SEQ ID NO: 757)               M734   GSTM4   glutathione   NM_000850   GCCAGAATACTTGGAGGAACTT                         S-transferase       TACAATGATGCAGCACTTCTCA                         M4       GT                       (SEQ ID NO: 758)               M735   GSTM5   glutathione   NM_000851   TTTCCTTGCCTATGATGTCCTTGA                         S-transferase       ATGAAGCGTATATTITGAGCCCAA                         M5       T                       (SEQ ID NO: 759)               M736   GSTP1   glutathione   NM_000852   GCTGCTGATCCATGAGGTCCTA                         S-transferase       CCCTGGCTGCCTGGATGCGTT               pi       (SEQ ID NO: 760)               M737   GSTT1   glutathione   NM_000853   AAACCTCACCCTTGCACCGTCCT                         S-transferase       AGCAGTCCACAAAGCATTTTCAT                         theta       TC                       (SEQ ID NO: 761)               M738   GSTZ1   glutathione   NM_145870   CATATACTGTGTAGGAGACGAG                         transferase       TGACCATGGCTGATCTGTGCTT                         zeta       T                       (SEQ ID NO: 762)               M739   MGST1   microsomal   NM_145792   GGAGTTACTCTTTCCATGGCTTA                         glutathione       AGGTTGCTGAAAAGTAAATTGTA                         S-transferase       CT               1       (SEQ ID NO: 763)               M740   MGST2   microsomal   NM_002413   CCAAGAAACTGAGGCGGCAATT                         glutathione       TAACTTTTTCTCTTCCCTTTAAT                         S-transferase       TT               2       (SEQ ID NO: 764)               M741   MGST3   microsomal   NM_004528   AAGTACAAAGTGGAGTATCCTA                         glutathione       ATGTACAGCACGGACCCTGAAA                         S-transferase       TGG               3       (SEQ ID NO: 765)               M742   AKR1A1   Ido-keto   NM_153326   ATCTGCATCCCCAAAAGTATCA                         reducta       CCTTCTCGAATCCTTCAGAACAT                         family 1,       AA               member A1       (SEQ ID NO: 766)               M743   ALDH1A   aldehyde   NM_000689   TGGTGGATTCAAGATGTCTGGAA                         dehydrogenase       TGGAAGAGAACTGGGAGAGTA                         family,       GTT               member A1       (SEQ ID NO: 767)               M744   ALDH1A   aldehyde   NM_170697   AAGCTGGGACTGTTTGGATCAAT                         dehydrogenase       GTTACAATGCCTTAAATGCCCA                         family,       GC               member A2       (SEQ ID NO: 768)               M745   ALDH1A   aldehyde   NM_000693   CAACATGCGGATTGCCAAAGAG                         dehydrogenase       AGATTTTCGGGCCAGTGCAACCA                         family,       TAC               member A3       (SEQ ID NO: 769)               M746   ALDH1B   aldehyde   NM_000692   AGAGGTAAAGACGGTCACCATC                         dehydrogenase       AGGTTCCTCAGAAGAACTCGTAA                         family,       GAGC               member B1       (SEQ ID NO: 770)               M747   ALDH2   aldehyde   NM_000690   GTCAAAGTGCCTCAGAAGAACT                         dehydrogenase       ATAAGAATCATGCAAGCTTCCT                         family       CTC                       (SEQ ID NO: 771)               M748   ALDH3A   aldehyde   NM_000691   CCTCTACATGTTCTCCAGCAAC                         dehydrogenase       CAAGGTGATTAAGAAGATGATT                         family,       CAG               member A1       (SEQ ID NO: 772)               M749   ALDH3A   aldehyde   NM_000382   GAATGATCCTGTTCAACCTCCTA                         dehydrogenase       TGCCTCTACTGAATTATTCCTCT                         family,       T               member A2       (SEQ ID NO. 773)               M750   ALDH4A   aldehyde   NM_003748   ATGCTGCCGGCAACTTCTACAT                         dehydrogenase       ACGACAAGTCCACTGGCTCGATA                         family,       GTG               member A1       (SEQ ID NO: 774)               M751   ALDH5A   aldehyde   NM_170740   ATGCTGTGCACTCATGAAGAGA                         dehydrogenase       TTCGGGCCTCTGGCACCAGTTAT                         family,       AA               member A1       (SEQ ID NO: 775)               M752   ALDH6A   aldehyde   NM_005589   AGGGCATCCAATFCTACACTCA                         dehydrogenase       TAAAGACCATTACTTCTCAGTG                         family,       AA               member A1       (SEQ ID NO: 776)               M753   ALDH7A   aldehyde   NM_001182   CTTGGACCTAAAGGATCAGACT                         dehydrogenase       GGCATTGTAAATGTCAACATTC                         family,       AC               member A1       (SEQ ID NO: 777)               M754   ALDH8A   aldehyde   NM_022568   CTGGAATAGGTAGAGAGGGAG                         dehydrogenase       AAGGACTCTTACGACTTCTTCA                         family,       GAG               member A1       (SEQ ID NO: 778)               M755   ALDH9A   aldehyde   NM_000696   TAGCTGAGCTTCAGGCTGGGAC                         dehydrogenase       GCTTCATTAACAACTATAACGT                         family,       GC               member A1       (SEQ ID NO: 779)               M756   GPX1   glutathione   NM_000581   TACGAGGGAGGAACACCTTGAT                         peroxidase 1       TTACAGAAAATACCACCTCGAG                                 GGG                       (SEQ ID NO: 780)               M757   GPX2   glutathione   NM_002083   AATGAGGAGATCCTGAACAGTC                         peroxidase 2       CAAGTATGTCCGTCCTGGGGGT                                 ATA                       (SEQ ID NO: 781)               M758   GPX3   glutathione   NM_002084   CAACCAATTTGGAAAACAGGAA                         peroxidase 3       CAGGAGAGAACTCAGAGATCCT                                 CCTA                       (SEQ ID NO: 782)               M759   GPX4   glutathione   NM_002085   TGTAACCAGTTCGGGAAGCAGG                         peroxidase 4       GCCAGGGAGTAACGAAGAGAT                                 AAGA                       (SEQ ID NO: 783)               M760   GPX5   glutathione   NM_001509   CTGAACTAAATGCACTCCAGGA                         peroxidase 5       GAGCTGAAGCCCTATGGTCTAGT                                 GTG                       (SEQ ID NO: 784)               M761   GPX6   glutathione   NM_015696   ACCTACAGTGTCTCATTCCCCAT                         peroxidase 6       TTTAGCAAGATTGCAGTCACCG                                 AC                       (SEQ ID NO: 785)                  
 
       EXAMPLE 10  
       [0000]     Analysis of Gene Expression  
         [0040]     The molecular chip showed distinctive skin patterns of transcriptional expression of functional neuro-endocrine-immune and barrier forming pathways after ultraviolet light exposure. The full list of differentially expressed genes is presented in Table 2, classified into several major functionally-related groups: water and salt balance; prostaglandin metabolism; interleukins and growth factors; metalloproteinases; markers of keratinocyte differentiation and stress response. Genes from the same group that were similarly expressed presumably reflected a highly coordinated keratinocyte response to UV, which indicated genetic regulation. Each gene had a unique time response that was classified as early, when the maximum induction of expression had occurred by 4 h after treatment. A late response group was defined by expression maximum occurring at 24 h after irradiation.  
       EXAMPLE 11  
       [0000]     Genes Involved in Water and Salt Balance  
         [0041]     Up-regulation of gastrin, vasopressin and aquaporin genes was detected. All these genes are involved in the regulation of water and salt balance (Table 2,  FIG. 2 ). The highest stimulation was observed at 12 to 24 h post-irradiation. Vasopressin is highly relevant to water metabolism by being involved in water resorption in the kidneys. Other genes related to water balance are those coding for aquaporin water channels (AQP). Aquaporins are a family of membrane proteins that facilitate water movement across biological membranes; they are important for osmotic water movement across cell membranes of epithelial and endothelial barriers. Aquaporins are also expressed in the skin. AQP3 knockout mice are remarkably polyuric and have dry skin (Verkman, 2002). The microarray data was verified by real-time quantitative RT-PCR, which showed 2-fold increases of aquaporin 3 mRNA ( FIG. 2A , p&lt;0.05).  
         [0042]     The gastrin gene is related to salt balance since gastrin receptors are expressed in the kidneys on tubules and collecting ducts, where they mediate renal potassium and sodium absorption (von Schrenck et al., 2000). Gastrin has potent growth stimulatory effects on gastrointestinal tissues and human kidney cells (Stepan et al., 1999). Gastrin-releasing peptide may also accelerate wound healing in burns, injuries, chronic ulcers, and skin graft donor sites through enhancement of keratinocyte growth and spreading (Yamaguchi et al., 2002). Since the gastrin message was not previously detected in skin, this gene was amplified herein from several skin cell lines including human keratinocytes, melanocytes, fibroblasts and different melanomas ( FIG. 2B ). The band of correct length was detected only in normal and immortalized keratinocytes ( FIG. 2B ); after sequencing, the gene was showed perfectly matched with gastrin. Thus, gastrin is expressed in human keratinocytes and its expression is stimulated by UVR (Table 2).  
                                                                             TABLE 2                           Genes differentially expressed in ultraviolet irradiated human epidermal keratinocytes            UP-REGULATED GENES   DOWN-REGULATED GENES                mean ± SD       mean ± SD            gene   4 h   12 h   24 h   gene   4 h   12 h   24 h               Water and salt balance                                   Gastrin   1.17 ± 0.35   2.06 ± 0.32   1.46 ± 0.17       Vasopressin precursor   1.04 ± 0.49   1.56 ± 0.01   1.54 ± 0.01       Aquaporin 1   1.53 ± 0.24   1.87 ± 0.37   4.11 ± 0.42       Aquaporin 3   1.11 ± 0.10   1.49 ± 0.09   2.37 ± 0.19       Aquaporin 9   1.04 ± 0.28   1.87 ± 0.42   2.05 ± 0.07       Prostaglandin metabolism       CYP4F8 (cytochrome P450,   1.57 ± 0.02   2.41 ± 0.32   3.15 ± 0.72       subfamily IVF, polypeptide 8)       Prostaglandin-endoperoxide   1.87 ± 0.25   3.61 ± 0.40   1.20 ± 0.16       synlhase 2 (COX-2)       Interleukins and growth factors       Interferon-beta-2   0.94 ± 0.33   1.61 ± 0.12   0.98 ± 0.04   Epidermal growth   1.31 ± 0.71   0.50 ± 0.10   1.00 ± 0.3       (interleukin 6)               factor receptor       Interleukin 1, beta   2.14 ± 0.07   2.22 ± 0.48   0.98 ± 0.10   Keratinocyte   1.07 ± 0.10   0.49 ± 0.10   0.74 ± 0.1                       growth factor       Interleukin 2   1.16 ± 0.25   1.13 ± 0.07   2.02 ± 0.10   Keratinocyte   0.78 ± 0.09   0.46 ± 0.04   0.59 ± 0.0       growth factor       Interleukin 8   2.44 ± 0.28   1.95 ± 0.12   1.70 ± 0.20   Activin A recept   0.58 ± 0.04   0.49 ± 0.07   0.99 ± 0.0                       type I           1.76 ± 0.08   2.10 ± 0.10   1.28 ± 0.22   TNFSF10 (TRAI   0.51 ± 0.03   0.65 ± 0.03   0.73 ± 0.0       TNFSF9       Bone morphogenetic   1.16 ± 0.06   1.67 ± 0.03   1.10 ± 0.06       protein 3                   Insulin-like growth   1.78 ± 0.24   0.98 ± 0.16   1.78 ± 0.42       factor binding       protein 6               Endothelin 1   2.30 ± 2.70   2.78 ± 4.10   2.10 ± 2.20       Vascular endothelial growth factor   1.10 ± 0.09   2.09 ± 0.08   1.85 ± 0.49       Metalloproteinases       Stromelysin 1 a     n/d   2.02 ± 0.03   n/d       Stromelysin 2 a     n/d   2.78 ± 0.93   n/d       Markers of keratinocyte       differentiation       Filaggrin   1.04 ± 0.21   1.57 ± 0.12   1.14 ± 0.04   Fibronectin 1   0.96 ± 0.04   0.62 ± 0.07   0.41 ± 0.0       Involucrin   1.05 ± 0.04   1.57 ± 0.08   1.46 ± 0.03       Keratin 1   1.26 ± 0.18   1.80 ± 0.17   1.57 ± 0.04       Keratin 10   1.18 ± 0.06   2.09 ± 0.43   0.98 ± 0.04       Keratin 19   0.92 ± 0.38   0.85 ± 0.23   3.17 ± 0.31       Transglutaminase 1   1.05 ± 0.15   1.18 ± 0.22   2.01 ± 0.23       Loricrin   1.29 ± 0.21   1.54 ± 0.24   0.51 ± 0.07       Stress-related genes       CRF-R2 gamma   1.10 ± 0.08   1.99 ± 0.45   1.60 ± 0.07   Urotensin 2   1.10 ± 0.08   0.81 ± 0.13   0.62 ± 0.0       Aldo-keto reductase family 1,   1.10 ± 0.08   1.25 ± 0.12   1.56 ± 0.05       member C2                   a effect was seen only when keratinocytes were co-cultivated with melanocytes; n/d—not done; data represents mean +/− SD (N = 4); underlined ratios (UV versus control) are significantly different from 1 (no difference), P &lt; 0.05.             
 
       EXAMPLE 12  
       [0000]     Prostaglandin Synthesis  
         [0043]     Up-regulation of several genes involved in prostaglandin metabolism including prostaglandin-endoperoxide synthase 2 (Cyclooxygenase 2 or COX-2 gene) (Table 2,  FIG. 2 ); and, of cytochrome P450, subfamily IVF, polypeptide 8 (CYP4F8) (Table 2) were detected. This was consistent with the increase in prostaglandin E2 in UVR-induced inflammation in the skin (Gresham et al., 1996). Real-time PCR also showed a 6-fold increase of COX-2 message ( FIG. 2 ), which was in fact the highest up-regulation ever detected by quantitative PCR. Stimulation of COX-2 expression by UVR had been previously reported (Brecher, 2002) and when taken together with findings presented here, indicated de novo synthesis of prostaglandins. Therefore, the increases in prostaglandin levels in inflammation would be due to increased prostaglandin production coupled with enhanced arachidonate deacylation from the membrane (Gresham et al., 1996).  
         [0044]     Another prostaglandin-related gene CYP4F8 was also up-regulated in UV-irradiated keratinocytes (Table 2). That gene, expressed both in human seminal vesicles and skin, catalyzes the 19-hydroxylation of prostaglandins H(1) and H(2) (Stark et al., 2003). CYP4F8 immunoreactivity and mRNA are prominently induced in psoriasis suggesting a pathophysiological role in skin (Stark et al., 2003).  
         [0045]     Prostaglandins function has been investigated on melanocytes and keratinocytes. Prostaglandins can induce melanin synthesis in cutaneous melanocytes. Uveal melanocytes also demonstrate increased tyrosinase activity upon exposure to prostaglandin E1 (Fuller and Meyskens, 1981). Prostaglandin E2 receptor agonist has stimulating effects on growth, melanogenesis and dendrification of iridal melanocytes (Hu et al., 2001). In keratinocytes prostaglandins increase vascular endothelial growth factor (VEGF) expression (Trompezinski et al., 2001), the latter a well-recognized effect of UV radiation (Mildner et al., 1999).  
       EXAMPLE 13  
       [0000]     Interleukins and Growth Factors  
         [0046]     Ultraviolet B radiation generates cutaneous inflammation in part, by inducing oxidative stress and cytokine production in human keratinocytes. Expression of the cytokines interleukin (IL)-1, IL-6, IL-10, endothelin 1, VEGFB has already been reported to increase in epidermal keratinocytes upon UV radiation (Kirnbauer et al., 1991; Grewe et al., 1995; Kang et al., 1998; Petit-Frere et al., 1998; Mildner et al., 1999; Brink et al., 2000). An increase of message corresponding to these genes (Table 2) was detected. Additionally, the microarray showed stimulation of IL-2, IL-8, tumor necrosis factor superfamily member 9 (TNFSF9), bone morphogenetic protein 3 (BMP3) and insulin-like growth factor binding protein 6 (IGFBP6) (Table 2).  
         [0047]     Decreased expression was noted for the following genes in UV-treated keratinocytes (Table 2): epidermal growth factor receptor (EGFR or erbB), keratinocyte growth factor (KGF or fibroblast growth factor 7), keratinocyte growth factor receptor (KGFR or fibroblast growth factor receptor 2), Activin A receptor type I and tumor necrosis factor superfamily member 10 (TNFSF10 or TRAIL).  
         [0048]     The majority of the gene expression changes that were detected herein had been already reported in UV-irradiated skin cells. Therefore, real-time RT-PCR was used only to verify the novel TNFSF9, KGFR and KGF changes in gene expression. TNFSF9 increased 3-fold ( FIG. 2A , p&lt;0.05); KGFR gene expression was significantly inhibited in UV-irradiated keratinocytes and to even more significant degree in co-cultures of keratinocytes and melanocytes ( FIG. 2A , p&lt;0.05). KGF was also down-regulated (data not shown), but since original expression of this gene was low, it was technically difficult to amplify the amount of message to allow analysis of the data in a statistically meaningful manner.  
         [0049]     The functional significance of the differential gene expression could be explained as follows. IL-8 is a neutrophil chemoattractant cytokine with proinflammatory and growth-promoting activities, and is involved in the pathogenesis of several inflammatory diseases. IL-8 is produced in response to ultraviolet B radiation (Countryman et al., 2000). Tumor necrosis factor superfamily member 9 (TNFSF9 or 4-1BBL) is also an important proinflammatory mediator binding CD137 receptors on activated CD4 and CD8 T cells. TNSF9 provides a costimulatory signal that induces a high level of IL-2 production by primary resting T cells (DeBenedette et al., 1999).  
         [0050]     Within this context, it would be anticipated that IL-2 expression would increase after UVR, as was indeed found (Table 2). Investigation of infiltrating inflammatory cells and cytokine levels in UV-irradiated skin has shown that interferon-gamma and IL-2, together with the proinflammatory cytokine IL-8, become detectable at 6 h (Terui et al., 2001). In the present invention,-IL-2 was detected 24 h after radiation and most interestingly only after up-regulation of TNFSF9 (4, 12 h; Table 2). This might reflect autocrine stimulation of IL-2 by TNFSF9. The significance of this response is underlined by the effectiveness of interferons and IL-2 in the treatment of skin cancers that include metastatic melanomas (Garbe, 1995).  
         [0051]     Stimulation of bone morphogenic protein 3 (BMP-3) and insulin-like growth factor binding protein 6 (IGFBP-6) was detected. Both these proteins inhibit proliferation and differentiation. Treatment of human bone marrow osteoprogenitors with BMP-3 causes dose- and time-dependent inhibition of DNA synthesis and cell proliferation. Simultaneously, BMP3 stimulates type I collagen synthesis (Amedee et al., 1994). IGFBP-6 is a negative effector of oligodendrocyte survival and differentiation (Kuhl et al., 2003), and is secreted by an immortalized human keratinocyte cell line (HaCaT) (Marinaro et al., 1999). Purified IGFBP-6 inhibits the growth of HaCaT and Balb/MK keratinocyte cell lines, as well as Mv1Lu cells (Kato et al., 1995).  
         [0052]     EGFR overexpression is important for the expression of malignant behavior, since activation of the receptor stimulates tyrosine kinase activity, which initiates signal transduction cascade (including other kinases) leading to cell proliferation and protection from apoptosis (Lage et al., 2003). Conversely, anti-EGFR agents posses antitumor activity (Lage et al., 2003). Within this context, the known effect of EGF stimulating extracellular receptor-activated kinases (ERK) is prevented by UVR, in a dose-dependent manner. Therefore, the data presented herein on UVR-induced transient (12 hours) inhibition of EGFR expression, suggested that this effect on EGFR was the most likely explanation of the described ERK response inhibition.  
         [0053]     Down-regulation of the tumor necrosis factor superfamily member 10 (TNFSF10 or TRAIL), which is a TNF-related apoptosis-inducing ligand was observed. Both up- and down-regulation of TRAIL and its receptors had been reported after exposure to UVB. Since TRAIL and TRAIL receptors function as important sensors in the human epidermis preserving skin integrity and preventing cell transformation, down-regulation of these molecules with sensor function increases the risk for less efficient elimination of aberrant cells. Supporting this concept is the low amount or absence of these molecules in precancerous states developing after long-term ultraviolet exposure (Bachmann et al., 2001).  
         [0054]     Keratinocyte growth factor (KGF or fibroblast growth factor 7) is a potent mitogen for human keratinocytes, stimulating proliferation and migration of these cells and also affecting their differentiation process (Werner, 1998). Deletion of the KGF receptor (KGFR) gene results in severe functional defects of the anterior pituitary and also, in developmental abnormalities of the skin (De Moerlooze et al., 2000). IL-6, interferon-gamma, and ultraviolet B (UVB) treatment lead to substantial down-regulation of KGFR expression (Zhou et al., 1996). Down regulation of both KGF and KGFR (Table 2) was detected, confirmed by real-time PCR ( FIG. 2A ). The UV-induced down-regulation of KGF and KGFR synthesis might therefore represent an antiproliferative mechanism addressed at preventing cells from entering malignant transformation.  
       EXAMPLE 14  
       [0000]     Genes Involved in Extracellular Matrix Rearrangement  
         [0055]     Involvement of matrix metalloproteinases (MMPs), the enzymes responsible for extracellular matrix rearrangement, UVR-induced skin aging and promotion of skin cancer (Brenneisen et al., 2002), was also observed. This was represented by a significant stimulation of stromelysins 1 and 2 expression, but only when keratinocytes were co-cultivated with melanocytes (Table 2). Real-time quantitative RT-PCR did confirm this observation ( FIG. 2A , p&lt;0.05), suggesting that either keratinocytes express UV-induced metalloproteinases only when they are in contact with melanocytes or, that the matrix metalloproteinases are produced by melanocytes.  
       EXAMPLE 15  
       [0000]     Stress Response Genes  
         [0056]     CRH may be the primary mediator of autonomic, behavioral and neuroendocrine responses to stress. While CRH receptors type 1 were previously detected exclusively in human skin cells (Slominski et al., 2004), the CRH receptor type 2 gamma isoform, whose expression is increased in UVR-treated human keratinocytes were included in the present invention.  
         [0057]     Aldo-keto reductase, another stress-response related gene, was observed to be up-regulated. Interestingly, the fungi  S. cerevisiae  that have aldo-keto reductases deleted show enhanced sensitivity to stress (Chang et al., 2003).  
         [0058]     The following references were cited herein: 
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         [0098]     Any patents or publications mentioned in this specification are indicative of the levels of those skilled in the art to which the invention pertains. Further, these patents and publications are incorporated by reference herein to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference.