PATENT DOCUMENT

Abstract:
This invention relates to DNA sequences encoding sucrose phosphate synthase which can be integrated into a plant genome and which modify the activity of the sucrose-phosphate-synthase (sps) of the plant. Methods for transforming plant cells and regenerating plants from the cells which exhibit modified sucrose-phosphate-synthase activity are also described.

Full Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application is a divisional of application Ser. No. 08/778,656 filed Jan. 3, 1997, now U.S. Pat. No. 5,976,869, which issued on Nov. 2, 1999, which is a divisional of application Ser. No. 08/356,354 filed Dec. 20, 1994, now U.S. Pat. No. 5,767,365, which issued on Jun. 16, 1998, which is a continuation of PCT/EP93/01605 filed Jun. 22, 1993. The disclosure of these applications are hereby incorporated by reference into the present application. 
    
    
     FIELD OF THE INVENTION 
     The present invention relates to DNA sequences and plasmids, containing these DNA sequences, which by integration into a plant genome, cause the activity of the sucrose-phosphate-synthase (SPS) of the plant to be changed and thus affect the sugar metabolism of the plant. The invention further relates to transgenic plants, in which through introduction of the DNA sequences, changes in the activity of the sucrose-phosphate-synthase are produced. 
     BACKGROUND OF THE INVENTION 
     Sucrose is of central importance for the plant and serves many functions. For the long distance transport of photoassimilates and/or energy between various organs in plants, sucrose is almost exclusively used. The sucrose which is transported in a specific heterotrophic organ determines the growth and the development of this organ. Thus it is known, e.g. from EP 442 592, that transgenic plants, in which the transport of the sucrose away from the exporting leaves is inhibited by expression of an apoplastic invertase, shows a strong reduction in the growth of e.g. roots or tubers in the case of potato plants. For tobacco plants, the principal importance of sucrose for the long distance transport of energy carriers within the plant is described in von Schaewen et al, 1990, EMBO J 9: 3033-3044. 
     While it has been clearly shown that a reduction of the amount of sucrose imported in the heterotrophic organs, such as tubers and seeds, leads to loss of yield, it is not known whether an increase in the amount of sucrose in the photosynthetically active parts of the plant, mainly the leaves, leads to a better supply of heterotrophic organs and thus to an increase in yield. 
     A second central role for sucrose and/or the hexoses, glucose and fructose which are derived from sucrose, is in the protection of plants against frost damage at low temperatures. Frost damage is one of the main limiting factors in agricultural productivity in the northern hemisphere. Temperatures below freezing lead to the formation of ice crystals. Since the growing ice crystals consist of pure water, water is abstracted from the cells as the temperature falls. 
     This dehydration has at least two potential damaging results: 
     a) all dissolved substances within a cell are strongly concentrated and the cell contracts following the loss of water. Highly concentrated salts and organic acids lead to membrane damage; 
     b) with rehydration from dew, the previously contacted cells reexpand. The cell membrane also expands again. The volume expansion puts a heavy mechanical load on the membrane. 
     It is thus clear that a freezing/dew cycle can lead to severe membrane damage of the cells and thus to damage to the plant. 
     It thus appears worth trying to hinder the freezing. One possible strategy is to increase the formation of osmotically active substances in the cytosol of plant cells. This should lead to a lowering of the freezing point. Osmotically active substances include sucrose and/or the two hexoses which are derived from sucrose. 
     The increased formation of sucrose and/or the two hexoses at low temperatures is desirable in the growing plant. Another situation can exist in the harvested parts of a plant, especially in storage. For example, in potato tubers that are stored at 4-8° C., hexoses (glucose) accumulate. It would appear to be sensible, to see this as the answer to a lowering of the temperature (“cold-sweetening”). 
     The accumulation of sucrose and glucose has in the case of potato tubers economically undesirable results. Increased amounts of reducing sugars, such as glucose, in potatoes which are fried when preparing crisps, chips and the like, leads to an undesirable browning due to the Maillard reaction. Such products with a dark brown color are not generally acceptable to the consumer. Further the cooking strength is strongly dependent on the content of starch and/or its breakdown products which are important in determining the quality characteristics of the potato. 
     In relation to the economic aspects, sucrose thus possesses three especially important functions: 
     1 as the transport form for the distant transport of photoassimilates, 
     2 as an osmotically active substance with the desirable activity of lowering the freezing point in intact, growing plants, and 
     3 in the undesirable formation of reducing sugars in stored harvested parts of a plant, e.g. the potato tubers, as a result of low temperatures. 
     The biosynthesis pathways for the formation of sucrose, either from the primary photosynthesis products (in the leaf) or by breakdown of starch (in the storage organs e.g. of potatoes), are known. An enzyme in sucrose metabolism is sucrose-phosphate-synthase (SPS). It forms sucrose-6-phosphate from UDP-glucose and fructose-6-phosphate, which in a second step is converted to sucrose. 
     The isolation of SPS from maize and the cloning of cDNA from mRNA from maize tissue is known (EP 466 995). In this application, processes for the purification of a protein such as by centrifuging or homogenates, differential precipitation and chromatography are described. A 300 times enrichment of SPS from plant tissue has been described by Salerno and Pontis (Planta 142: 41-48, 1978). 
     In view of the significance of SPS for carbohydrate metabolism, it is questionable whether plants can tolerate a reduction in SPS activity in all or in certain organs. It is especially not known whether it is possible to produce transgenic plants with a reduced SPS activity. Also the use of SPS for the modification of the functions of sucrose for lowering the freezing point in intact plants and for the formation of reducing sugars in harvested parts is not known. 
     For the preparation of plants with reduced SPS activity, i.e. plants with changed sucrose concentration, it is necessary to make available an SPS coding region of such plant species, for which processes are described, whereby transgenic plants can be grown in large numbers. In as much as a reduction of SPS activity can be achieved, by selection from a large amount, the possibility exists of obtaining plants with such a phenotype. Further organ specific promoters for gene expression should exist for the plant species, by which the possibility of an organ specific reduction of the SPS activity could be investigated. 
     A species which fulfils the stated requirements is  Solanum tuberosum . The genetic modification of  Solanum tuberosum  by gene transfer using Agrobacteria is well described (Fraley et al., 1985, Crit Rev Plant Sci 4: 1-46). Promoters for leaf specific (Stockhaus et al., 1989, Plant Cell 1: 805-813), tuber specific (EP 375 092) and wound inducing (EP 375 091) gene expression are known. 
     SUMMARY OF THE INVENTION 
     The present invention now provides DNA sequences with which changes of SPS activity are actually and demonstrably possible and with which the sucrose concentration in the plant can be modified. It is concerned with sequences which include the coding region of sucrose-phosphate-synthase (SPS) from  Solanum tuberosum.    
     These DNA sequences can be introduced in plasmids and thereby combined with steering elements for expression in eukaryotic cells. Such steering elements are, on the one hand, transcription promoters and, on the other hand, transcription terminators. 
     Each plasmid comprises: 
     a) a suitable promoter that ensures that the coding sequence is read off at the suitable time point and/or in a specified development stage in the transgenic plants or in specified tissues of transgenic plants, 
     b) at least one coding sequence, that 
     i) is coupled to the promoter so that RNA can be translated into protein, whereby the protein demonstrates enzymatic activity, that leads to a modification of the sucrose concentration in the plant, or 
     ii) is coupled to the promoter so that the non-coding strand is read off, which leads to the formation of a so-called “anti-sense” RNA, which suppresses the formation of the coding protein of an endogenous gene in the plant which is involved in the sucrose biosynthesis, and 
     c) a non-coding termination sequence that contains the signals for the termination and polyadenylation of the transcript. 
     The present invention further provides plasmids in which include DNA sequences which change the SPS activity in the plant. 
     The coding sequences named under b) include the SPS sequences with the following nucleotide sequences: 
     
       
         
               
               
               
             
           
               
                 SPS 1 sequence (Seq. ID. No. 2): 
                   
                   
               
               
                 CTATTCTCTC CCCTCCTTTT TCTCCTCTCT TCAACCCCAA AACTTCCCTT TCAAAGCCTT 
                 60 
               
               
                   
               
               
                 TGCTTTCCCT TTCTCACTTA CCCAGATCAA CTAAGCCAAT TTGCTGTAGC CTCAGAAAAC 
                 120 
               
               
                   
               
               
                 AGCATTCCCA GATTGAAAAA GAATCTTTTT CAGTACCCAA AAGTTGGGTT TCTCATGTCC 
                 180 
               
               
                   
               
               
                 AGCAAGGATT AGCTGCTCTA GCTATTTCTT TAGCCCTTAA TTTTTGTCCA GTTGTGTCTT 
                 240 
               
               
                   
               
               
                 CTGATTCTGC ATTGGCATCT GAATTTGATG TGTTAAATGA AGGGCCACCA AAGGACTCAT 
                 300 
               
               
                   
               
               
                 ATGTAGTTGA TGATGCTGGT GTGCTTAGCA GGGTGACAAA GTCTGATTTG AAGGCATTGT 
                 360 
               
               
                   
               
               
                 TGTCTGATGT GGAGAAGAGA AAAGGCTTCC ACATTAATTT CATCACTGTC CGCAAGCTCA 
                 420 
               
               
                   
               
               
                 CTAGCAAAGC TGATGCTTTT GAGTATGCTG ACCAAGTTTT GGAGAAGTGG TACCCTAGTG 
                 480 
               
               
                   
               
               
                 TTGAACAAGG AAATGATAAG GGTATAGTTG TGCTTGTTAC AAGTCAAAAG GAAGGCGCAA 
                 540 
               
               
                   
               
               
                 TAACCGGTGG CCCTGATTTT GTAAAGGCCG TTGGAGATAC TGTTCTTGAT GCTACCGTCT 
                 600 
               
               
                   
               
               
                 CAGAGAACCT TCCAGTGTTG GCTACTGAAG AGAAGTACAA TGAAGCAGTT TTCAGCACTG 
                 660 
               
               
                   
               
               
                 CCACACGTCT TGTTGCAGCC ATTGATGGCC TTCCTGATCC TGGTGGACCC CAACTCAAGG 
                 720 
               
               
                   
               
               
                 ATAACAAAAG AGAGTCCAAC TTCAAATCCA GAGAGGAAAC TGATGAGAAA AGAGGACAAT 
                 780 
               
               
                   
               
               
                 TCACACTTGT GGTTGGTGGG CTGTTAGTGA TTGCTTTTGT TGTTCCTATG GCTCAATACT 
                 840 
               
               
                   
               
               
                 ATGCATATGT TTCAAAGAAG TGAACTGTCT GATTCTGGAA AGTTACATTT TCGTGAGATT 
                 900 
               
               
                   
               
               
                 TGAGTAAGCA TGTATATTAT CGTGTACAAA ATGGTCCATT CGGAAATGAC TGATTC 
                 956 
               
               
                   
               
               
                 ATG AGA TAT TTA AAA AGG ATA AAT ATG AAG ATT TGG ACC TCC CCT 
                 1001 
               
               
                 Met Arg Tyr Leu Lys Arg Ile Asn Met Lys Ile Trp Thr Ser Pro 
               
               
                 1               5                    10                  15 
               
               
                   
               
               
                 AAC ATA ACG GAT ACT GCC ATT TCT TTT TCA GAG ATG CTG ACG CAA 
                 1046 
               
               
                 Asn Ile Thr Asp Thr Ala Ile Ser Phe Ser Glu Met Leu Thr Pro 
               
               
                                 20                  25                  30 
               
               
                   
               
               
                 ATA AGT ACA GAC GGC TTG ATG ACT GAG ATG GGG GAG AGT AGT GGT 
                 1091 
               
               
                 Ile Ser Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly 
               
               
                                 35                  40                  45 
               
               
                   
               
               
                 GCT TAT ATT ATT CGC ATT CCT TTT GGA CCA AGA GAG AAA TAT ATT 
                 1136 
               
               
                 Ala Tyr Ile Ile Arg Ile Pro Phe Gly Pro Arg Glu Lys Tyr Ile 
               
               
                                 50                  55                  60 
               
               
                   
               
               
                 CCA AAA GAA CAG CTA TGG CCC TAT ATT CCC GAA TTT GTT GAT GGT 
                 1181 
               
               
                 Pro Lys Glu Gln Leu Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly 
               
               
                                 65                  70                  75 
               
               
                   
               
               
                 GCA CTT AAC CAT ATT ATT CAA ATG TCC AAA GTT CTT GGG GAG CAA 
                 1226 
               
               
                 Ala Leu Asn His Ile Ile Gln Met Ser Lys Val Leu Gly Glu Gln 
               
               
                                 80                  85                  90 
               
               
                   
               
               
                 ATT GGT AGT GGC TAT CCT GTG TGG CCT GTT GCC ATA CAC GGA CAT 
                 1271 
               
               
                 Ile Gly Ser Gly Tyr Pro Val Trp Pro Val Ala Ile His Gly His 
               
               
                                 95                  100                 105 
               
               
                   
               
               
                 TAT GCT GAT GCT GGC GAC TCA GCT GCT CTC CTG TCA GGT GCT TTA 
                 1316 
               
               
                 Tyr Ala Asp Ala Gly Asp Ser Ala Ala Leu Leu Ser Gly Ala Leu 
               
               
                                 110                 115                 120 
               
               
                   
               
               
                 AAT GTA CCA ATG CTT TTC ACT GGT CAC TCA CTT GGT AGA GAT AAG 
                 1361 
               
               
                 Asn Val Pro Met Leu Phe Thr Gly His Ser Leu Gly Arg Asp Lys 
               
               
                                 125                 130                 135 
               
               
                   
               
               
                 TTG GAG CAA CTG TTG CGA CAA GGT CGT TTG TCA AAG GAT GAA ATA 
                 1406 
               
               
                 Leu Glu Gln Leu Leu Arg Gln Gly Arg Leu Ser Lys Asp Glu Ile 
               
               
                                 140                 145                 150 
               
               
                   
               
               
                 AAC TCA ACC TAC AAG ATA ATG CGG AGA ATA GAG GCT GAA GAA TTA 
                 1451 
               
               
                 Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala Glu Glu Leu 
               
               
                                 155                 160                 165 
               
               
                   
               
               
                 ACT CTT GAT GCT TCC GAA ATT GTC ATC ACT AGT ACA AGA CAG GAG 
                 1496 
               
               
                 Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser Thr Arg Gln Glu 
               
               
                                 170                 175                 180 
               
               
                   
               
               
                 ATT GAC GAG CAA TGG CGT TTG TAT GAT GGG TTT GAT CCA ATA TTA 
                 1541 
               
               
                 Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp Pro Ile Leu 
               
               
                                 185                 190                 195 
               
               
                   
               
               
                 GAG CGT AAG TTA CGT GCA AGG ATC AAG CGC AAT GTC AGC TGT TAT 
                 1586 
               
               
                 Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser Cys Tyr 
               
               
                                 200                 205                 210 
               
               
                   
               
               
                 GGC AGG TTT ATG CCT CGT ATG GCT GTA ATT CCT CCT GGG ATG GAG 
                 1631 
               
               
                 Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met Glu 
               
               
                                 215                 220                 225 
               
               
                   
               
               
                 TCC CAC CAT ATT GTG CCA CAT GAA GGT GAC ATG GAT GGA GAA ACA 
                 1676 
               
               
                 Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr 
               
               
                                 230                 235                 240 
               
               
                   
               
               
                 GAA GGA AGT GAA GAT GGG AAG ACC CCG GAT CCA CCT ATT TGG GCA 
                 1721 
               
               
                 Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala 
               
               
                                 245                 250                 255 
               
               
                   
               
               
                 GAG ATT ATG CGC TTC TTT TCT ATT CCA AGG AAG CCT ATG ATA CTC 
                 1766 
               
               
                 Glu Ile Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu 
               
               
                                 260                 265                 270 
               
               
                   
               
               
                 GCA CTT GCT AGG CCT GAT CCC AAG AAG AAC CTC ACT ACT TTA GTG 
                 1811 
               
               
                 Ala Leu Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val 
               
               
                                 275                 280                 285 
               
               
                   
               
               
                 AAA GCA TTT GGT GAA TGT CGT CCA TTG AGA GAG CTT GCT AAT CTT 
                 1856 
               
               
                 Lys Ala Phe Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu 
               
               
                                 290                 295                 300 
               
               
                   
               
               
                 ACT TTG ATA ATG GGT AAT CGA GAT AAT ATC GAC GAA ATG TCT AGC 
                 1901 
               
               
                 Thr Leu Ile Met Gly Asn Arg Asp Asn Ile Asp Glu Met Ser Ser 
               
               
                                 305                 310                 315 
               
               
                   
               
               
                 ACC AAT TCT GCA CTT CTT CTT TCA ATC TTG AAA ATG ATA GAT AAG 
                 1946 
               
               
                 Thr Asn Ser Ala Leu Leu Leu Ser Ile Leu Lys Met Ile Asp Lys 
               
               
                                 320                 325                 330 
               
               
                   
               
               
                 TAT GAT CTT TAT GGT CAA GTA GCT TAT CCT AAA CAC CAC AAG CAG 
                 1991 
               
               
                 Tyr Asp Leu Tyr Gly Gln Val Ala Tyr Pro Lys His His Lys Gln 
               
               
                                 335                 340                 345 
               
               
                   
               
               
                 TCA GAT GTT CCT GAT ATC TAC CGT CTT GCT GCA AAG ACT AAG GGT 
                 2036 
               
               
                 Ser Asp Val Pro Asp Ile Tyr Arg Leu Ala Ala Lys Thr Lys Gly 
               
               
                                 350                 355                 360 
               
               
                   
               
               
                 GTT TTT ATT AAT CCA GCT TTT ATT GAG CCT TTT GAA CTG ACT TTG 
                 2081 
               
               
                 Val Phe Ile Asn Pro Ala Phe Ile Glu Pro Phe Gly Leu Thr Leu 
               
               
                                 365                 370                 375 
               
               
                   
               
               
                 ATT GAG GCA GCA GCT TAT GGT CTC CCA ATG GTA GCC ACA AAA ATT 
                 2126 
               
               
                 Ile Glu Ala Ala Ala Tyr Gly Leu Pro Met Val Ala Thr Lys Asn 
               
               
                                 380                 385                 390 
               
               
                   
               
               
                 GGA GGA CCT GTT GAT ATA CAT AGG GTT CTT GAC AAT GGT CTC TTA 
                 2171 
               
               
                 Gly Gly Pro Val Asp Ile His Arg Val Leu Asp Asn Gly Leu Leu 
               
               
                                 395                 400                 405 
               
               
                   
               
               
                 GTG GAT CCC CAT GAT CAG CAG GCA ATT GCT GAT GCT CTT TTG AAG 
                 2216 
               
               
                 Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp Ala Leu Leu Lys 
               
               
                                 410                 415                 420 
               
               
                   
               
               
                 TTG GTT GCT GAT AAG CAA CTG TGG GCT AAA TGC AGG GCA AAT GGA 
                 2261 
               
               
                 Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg Ala Asn Gly 
               
               
                                 425                 430                 435 
               
               
                   
               
               
                 TTA AAA AAT ATC CAC CTT TTC TCA TGG CCC GAG CAC TGT AAA ACT 
                 2306 
               
               
                 Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys Lys Thr 
               
               
                                 440                 445                 450 
               
               
                   
               
               
                 TAT CTA TCC CGG ATA GCT AGC TGC AAA CCA AGG CAA CCA CGC TGG 
                 2351 
               
               
                 Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln Pro Arg Trp 
               
               
                                 455                 460                 465 
               
               
                   
               
               
                 CTG AGA TCC ATT GAT GAT GAT GAT GAA AAT TCA GAA ACA GAT TCA 
                 2396 
               
               
                 Leu Arg Ser Ile Asp Asp Asp Asp Glu Asn Ser Glu Thr Asp Ser 
               
               
                                 470                 475                 480 
               
               
                   
               
               
                 CCT AGT GAT TCC TTG AGA GAT ATT CAT GAT ATA TCT CTG AAT TTG 
                 2441 
               
               
                 Pro Ser Asp Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu 
               
               
                                 485                 490                 495 
               
               
                   
               
               
                 AGA TTT TCA TTA GAT GGG GAA AAG AAT GAC AAT AAA GAA AAT GCT 
                 2486 
               
               
                 Arg Phe Ser Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala 
               
               
                                 500                 505                 510 
               
               
                   
               
               
                 GAT AAT ACA TTA GAC CCC GAA GTT CGA AGG AGC AAG TTA GAG AAT 
                 2531 
               
               
                 Asp Asn Thr Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn 
               
               
                                 515                 520                 525 
               
               
                   
               
               
                 GCT GTT TTG TCC TTA TCT AAG GGT GCA CTG AAG AGC ACA TCA AAA 
                 2576 
               
               
                 Ala Val Leu Ser Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys 
               
               
                                 530                 535                 540 
               
               
                   
               
               
                 TCT TGG TCG TCA GAC AAG GCA GAC CAA AAC CCT GGT GCT GGT AAA 
                 2621 
               
               
                 Ser Trp Ser Ser Asp Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys 
               
               
                                 545                 550                 555 
               
               
                   
               
               
                 TTC CCA GCG ATT AGG AGG AGG CGA CAT ATT TTT GTT ATT GCA GTG 
                 2666 
               
               
                 Phe Pro Ala Ile Arg Arg Arg Arg His Ile Phe Val Ile Ala Val 
               
               
                                 560                 560                 565 
               
               
                   
               
               
                 GAT TGT GAT GCT AGC TCA GGA CTC TCT GGA AGT GTG AAA AAG ATA 
                 2711 
               
               
                 Asp Cys Asp Ala Ser Ser Gly Leu Ser Gly Ser Val Lys Lys Ile 
               
               
                                 570                 575                 580 
               
               
                   
               
               
                 TTT GAG GCT GTA GAG AAG GAA AGG GCA GAG GGT TCC ATT GGA TTT 
                 2756 
               
               
                 Phe Glu Ala Val Glu Lys Glu Arg Ala Glu Gly Ser Ile Gly Phe 
               
               
                                 585                 590                 595 
               
               
                   
               
               
                 ATC CTG GCT ACA TCT TTC AAT ATA TCA GAA GTA CAG TCT TTC CTG 
                 2801 
               
               
                 Ile Leu Ala Thr Ser Phe Asn Ile Ser Glu Val Gln Ser Phe Leu 
               
               
                                 600                 605                 610 
               
               
                   
               
               
                 CTT TCA GAG GGC ATG AAT CCT ACT GAT TTT GAT GCT TAC ATA TGC 
                 2846 
               
               
                 Leu Ser Glu Gly Met Asn Pro Thr Asp Phe Asp Ala Tyr Ile Cys 
               
               
                                 615                 620                 625 
               
               
                   
               
               
                 AAT AGT GGT GGT GAT CTT TAT TAT TCG TCC TTC CAT TCT GAG CAA 
                 2891 
               
               
                 Asn Ser Gly Gly Asp Leu Tyr Tyr Ser Ser Phe His Ser Glu Gln 
               
               
                                 630                 635                 640 
               
               
                   
               
               
                 AAT CCT TTT GTA GTT GAC TTG TAC TAT CAC TCA CAT ATT GAG TAT 
                 2936 
               
               
                 Asn Pro Phe Val Val Asp Leu Tyr Tyr His Ser His Ile Glu Tyr 
               
               
                                 645                 650                 655 
               
               
                   
               
               
                 CGT TGG GGG GGC GAA GGA TTG AGA AAG ACT TTG GTG CGT TGG GCC 
                 2981 
               
               
                 Arg Trp Gly Gly Glu Gly Leu Arg Lys Thr Leu Val Arg Trp Ala 
               
               
                                 660                 665                 670 
               
               
                   
               
               
                 GCC TCT ATC ATT GAT AAG AAT GGT GAA AAT GGA GAT CAC ATT GTT 
                 3026 
               
               
                 Ala Ser Ile Ile Asp Lys Asn Gly Glu Asn Gly Asp His Ile Val 
               
               
                                 675                 680                 685 
               
               
                   
               
               
                 GTT GAG GAT GAA GAC AAT TCA GCT GAC TAC TGC TAT ACT TTC AAA 
                 3071 
               
               
                 Val Glu Asp Glu Asp Asn Ser Ala Asp Tyr Cys Tyr Thr Phe Lys 
               
               
                                 690                 695                 700 
               
               
                   
               
               
                 GTC TGC AAG CCT GGG ACG GTT CCT CCA TCT AAA GAG CTT AGA AAA 
                 3116 
               
               
                 Val Cys Lys Pro Gly Thr Val Pro Pro Ser Lys Glu Leu Arg Lys 
               
               
                                 705                 710                 715 
               
               
                   
               
               
                 GTA ATG CGA ATT CAG GCA CTT CGT TGT CAC GCT GTT TAT TGT CAA 
                 3161 
               
               
                 Val Met Arg Ile Gln Ala Leu Arg Cys His Ala Val Tyr Cys Gln 
               
               
                                 720                 725                 730 
               
               
                   
               
               
                 AAT GGG AGT AGG ATT AAT GTG ATC CCT GTA CTG GCA TCT CGG TCC 
                 3206 
               
               
                 Asn Gly Ser Arg Ile Asn Val Ile Pro Val Leu Ala Ser Arg Ser 
               
               
                                 735                 740                 745 
               
               
                   
               
               
                 CAA GCA CTC AGG TAC TTA TAT CTG CGA TGG GGA ATG GAC TTG TCG 
                 3251 
               
               
                 Gln Ala Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser 
               
               
                                 750                 755                 760 
               
               
                   
               
               
                 AAG TTG GTG GTT TTC GTC GGA GAA AGT GGT GAT ACC GAT TAT GAA 
                 3296 
               
               
                 Lys Leu Val Val Phe Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu 
               
               
                                 765                 770                 775 
               
               
                   
               
               
                 GGA TTA ATC GGT GGT CTA CGC AAG GCT GTC ATA ATG AAA GGC CTC 
                 3341 
               
               
                 Gly Leu Ile Gly Gly Leu Arg Lys Ala Val Ile Met Lys Gly Leu 
               
               
                                 780                 785                 790 
               
               
                   
               
               
                 TGC ACT AAT GCA AGC AGC TTA ATT CAC GGT AAT AGG AAT TAC CCG 
                 3386 
               
               
                 Cys Thr Asn Ala Ser Ser Leu Ile His Gly Asn Arg Asn Tyr Pro 
               
               
                                 795                 800                 805 
               
               
                   
               
               
                 CTA TCT GAT GTT TTA CCA TTC GAC AGC CCT AAT GTC ATC CAA GCG 
                 3431 
               
               
                 Leu Ser Asp Val Leu Pro Phe Asp Ser Pro Asn Val Ile Gln Ala 
               
               
                                 810                 815                 820 
               
               
                   
               
               
                 GAC GAG GAA TGT AGC AGC ACC GAA ATC CGT TGC TTA CTG GTG AAA 
                 3476 
               
               
                 Asp Glu Glu Cys Ser Ser Thr Glu Ile Arg Cys Leu Leu Val Lys 
               
               
                                 825                 830                 835 
               
               
                   
               
               
                 CTA GCG GTA CTC AAA GGA TAATACCCTT CCCCCTTTGA TTGTCAAAAA 
                 3524 
               
               
                 Leu Ala Val Leu Lys Gly 
               
               
                                 840 
               
               
                   
               
               
                 CCTATATGAG CTATAAGACT ATGCCATGAA AAGAATGGCC ATCCATTTGG CTTGTCTTTT 
                 3584 
               
               
                   
               
               
                 GAAGCTGTTA ATACTTTTCA ACAGACTACA AAATGAGATG AGTCCTTTGA TCCTCTTTAA 
                 3644 
               
               
                   
               
               
                 AGGACATAAA AGCTTTATGC AAGAACCAGT GCTGTAAAGT TATAGAATTT CTTTTGCTAT 
                 3704 
               
               
                   
               
               
                 ATATGACATT CGACAGAACC TGTTCCGGTT CATCGA 
                 3740 
               
               
                   
               
               
                 SPS 2 sequence (Seq. ID No. 3 and No.4) 
               
               
                   
               
               
                 ATTTTTTTCT CTAAGTTCTC TCTCGCTGTC CTTATCATTT CACCACCTCC ATAAATCTAG 
                 60 
               
               
                   
               
               
                 AAACATCTTT TCTACTCCGT TAATCTCTCT AGCACACGGC GGAGGAGTGC GGCGGAGGAG 
                 120 
               
               
                   
               
               
                 ATG GCG GGA AAC GAT TGG ATT AAC AGT TAC TTA GAG GCG ATA CTG 
                 165 
               
               
                 Met Ala Gly Asn Asp Trp Ile Asn Ser Tyr Leu Glu Ala Ile Leu 
               
               
                 1               5                   10                  15 
               
               
                   
               
               
                 GAT GTT GGA CCA GGG CTA GAT GAT AAG AAG TCA TCG TTG TTG TTG 
                 210 
               
               
                 Asp Val Gly Pro Gly Leu Asp Asp Lys Lys Ser Ser Leu Leu Leu 
               
               
                                 20                  25                  30 
               
               
                   
               
               
                 AGA GAA AGA GGG AGG TTT AGT CCG ACG AGG TAC TTT GTT GAG GAA 
                 255 
               
               
                 Arg Glu Arg Gly Arg Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu 
               
               
                                 35                  40                  45 
               
               
                   
               
               
                 GTT ATT ACT GGA TTC GAT GAG ACT GAT TTG CAT CGT TCG TGG ATC 
                 300 
               
               
                 Val Ile Thr Gly Phe Asp Glu Thr Asp Leu His Arg Ser Trp Ile 
               
               
                                 50                  55                  60 
               
               
                   
               
               
                 CGA GCA CAA GCT ACT CGG AGT CCG CAG AGA AGG AAT ACT AGG CTC 
                 345 
               
               
                 Arg Ala Gln Ala Thr Arg Ser Pro Gln Arg Arg Asn Thr Arg Leu 
               
               
                                 65                  70                  75 
               
               
                   
               
               
                 GAG AAT ATG TGC TGG AGG ATT TGG AAT TTG GCT CGC CAG AAA AAG 
                 390 
               
               
                 Glu Asn Met Cys Trp Arg Ile Trp Asn Leu Ala Arg Gln Lys Lys 
               
               
                                 80                  85                  90 
               
               
                   
               
               
                 CAG CTT GAG GGA GAG CAA GCT CAG TGG ATG GCA AAA CGC CGT CAA 
                 435 
               
               
                 Gln Leu Glu Gly Glu Gln Ala Gln Trp Met Ala Lys Arg Arg Gln 
               
               
                                 95                  100                 105 
               
               
                   
               
               
                 GAA CGT GAA AGA GGT CCC AGA GAA GCA GTT GCT GAT ATG TCA GAG 
                 480 
               
               
                 Glu Arg Glu Arg Gly Arg Arg Glu Ala Val Ala Asp Met Ser Glu 
               
               
                                 110                 115                 120 
               
               
                   
               
               
                 GAT CTA TCT GAG GGA GAG AAA GGA GAT ATA GTC GCT GAC ATG TCA 
                 525 
               
               
                 Asp Leu Ser Glu Gly Glu Lys Gly Asp Ile Val Ala Asp Met Ser 
               
               
                                 125                 130                 135 
               
               
                   
               
               
                 TCT CAT GGT GAA AGT ACC AGA GGC CGA TTG CCT AGA ATC AGT TCT 
                 570 
               
               
                 Ser His Gly Glu Ser Thr Arg Gly Arg Leu Pro Arg Ile Ser Ser 
               
               
                                 140                 145                 150 
               
               
                   
               
               
                 GTT GAG ACA ATG GAA GCA TGG GTC AGT CAG CAG AGA GGA AAG AAG 
                 615 
               
               
                 Val Glu Thr Met Glu Ala Trp Val Ser Gln Gln Arg Gly Lys Lys 
               
               
                                 155                 160                 165 
               
               
                   
               
               
                 CTT TAT ATC GTG CTT ATA AGT TTA CAT GGT TTA ATT CGG GGT GAG 
                 660 
               
               
                 Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu Ile Arg Gly Glu 
               
               
                                 170                 175                 180 
               
               
                   
               
               
                 AAT ATG GAG CTT GGA CGG GAT TCT GAT ACT GGT GGT CAG GTG AAG 
                 705 
               
               
                 Asn Met Glu Leu Gly Arg Asp Ser Asp Thr Gly Gly Gln Val Lys 
               
               
                                 185                 190                 195 
               
               
                   
               
               
                 TAT GTT GTT GAA CTT GCG AGG GGC TTA GGG TCG ATG CCA GGT GTA 
                 750 
               
               
                 Tyr Val Val Glu Leu Ala Arg Ala Leu Gly Ser Met Pro Gly Val 
               
               
                                 200                 205                 210 
               
               
                   
               
               
                 TAT CGG GTT GAC TTG CTT ACT AGA CAA GTA TCT TCA CCA GAA GTA 
                 795 
               
               
                 Tyr Arg Val Asp Leu Leu Thr Arg Gln Val Ser Ser Pro Glu Val 
               
               
                                 215                 220                 225 
               
               
                   
               
               
                 GAT TGG AGC TAT GGT GAG CCG ACA GAG ATG CTG ACG CCA ATA AGT 
                 840 
               
               
                 Asp Trp Ser Tyr Gly Glu Pro Thr Glu Met Leu Thr Pro Ile Ser 
               
               
                                 220                 235                 240 
               
               
                   
               
               
                 ACA GAC GGC TTG ATG ACT GAG ATG GGG GAG AGT AGT GGT GCT TAT 
                 885 
               
               
                 Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly Ala Tyr 
               
               
                                 245                 250                 255 
               
               
                   
               
               
                 ATT ATT CGC ATT CCT TTT GGA CCA AGA GAG AAA TAT ATT CCA AAA 
                 930 
               
               
                 Ile Ile Arg Ile Pro Phe Gly Pro Arg Glu Lys Tyr Ile Pro Lys 
               
               
                                 260                 265                 270 
               
               
                   
               
               
                 GAA CAG CTA TGG CCC TAT ATT CCC GAA TTT GTT GAT GGT GCA CTT 
                 975 
               
               
                 Glu Gln Leu Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly Ala Leu 
               
               
                                 275                 280                 285 
               
               
                   
               
               
                 AAC CAT ATT ATT CAA ATG TCC AAA GTT CTT GGG GAG CAA ATT GGT 
                 1020 
               
               
                 Asn His Ile Ile Gln Met Ser Lys Val Leu Gly Glu Gln Ile Gly 
               
               
                                 290                 295                 300 
               
               
                   
               
               
                 AGT GGC TAT CCT GTG TGG CCT GTT GCC ATA CAC GGA CAT TAT GCT 
                 1065 
               
               
                 Ser Gly Tyr Pro Val Trp Pro Val Ala Ile His Gly His Tyr Ala 
               
               
                                 305                 310                 315 
               
               
                   
               
               
                 GAT GCT GGC GAC TCA GCT GCT CTC CTG TCA GGT GCT TTA AAT GTA 
                 1110 
               
               
                 Asp Ala Gly Asp Ser Ala Ala Leu Leu Ser Gly Ala Leu Asn Val 
               
               
                                 320                 330                 335 
               
               
                   
               
               
                 CCA ATG CTT TTC ACT GGT CAC TCA CTT GGT AGA GAT AAG TTG GAG 
                 1155 
               
               
                 Pro Met Leu Phe Thr Gly His Ser Leu Gly Arg Asp Lys Leu Glu 
               
               
                                 340                 345                 350 
               
               
                   
               
               
                 CAA CTG TTG GCA CAA GGT CGA AAG TCA AAG GAT GAA ATA AAC TCA 
                 1200 
               
               
                 Gln Leu Leu Ala Gln Gly Arg Lys Ser Lys Asp Glu Ile Asn Ser 
               
               
                                 355                 360                 365 
               
               
                   
               
               
                 ACC TAC AAG ATA ATG CGG AGA ATA GAG GCT GAA GAA TTA ACT CTT 
                 1245 
               
               
                 Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala Glu Glu Leu Thr Leu 
               
               
                                 370                 375                 380 
               
               
                   
               
               
                 GAT GCT TCC GAA ATT GTC ATC ACT AGT ACA AGA CAG GAG ATT GAC 
                 1290 
               
               
                 Asp Ala Ser Glu Ile Val Ile Thr Ser Thr Arg Gln Glu Ile Asp 
               
               
                                 385                 390                 395 
               
               
                   
               
               
                 GAG CAA TGG CGT TTG TAT GAT GGG TTT GAT CCA ATA TTA GAG CGT 
                 1335 
               
               
                 Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp Pro Ile Leu Glu Arg 
               
               
                                 400                 405                 410 
               
               
                   
               
               
                 AAG TTA CGT GCA AGG ATC AAG CGC AAT GTC AGC TGT TAT GGC AGG 
                 1380 
               
               
                 Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser Cys Tyr Gly Arg 
               
               
                                 415                 420                 425 
               
               
                   
               
               
                 TTT ATG CCT CGT ATG GCT GTA ATT CCT CCT GGG ATG GAG TTC CAC 
                 1425 
               
               
                 Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met Glu Phe His 
               
               
                                 430                 435                 440 
               
               
                   
               
               
                 CAT ATT GTG CCA CAT GAA GGT GAC ATG GAT GGT GAA ACA GAA GGA 
                 1470 
               
               
                 His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr Glu Gly 
               
               
                                 445                 450                 455 
               
               
                   
               
               
                 AGT GAA GAT GGG AAG ACC CCC GAT CCA CCT ATT TGG GCA GAG ATT 
                 1515 
               
               
                 Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu Ile 
               
               
                                 460                 465                 470 
               
               
                   
               
               
                 ATG CGC TTC TTT TCT AAT CCA AGG AAG CCT ATG ATA CTC GCA CTT 
                 1560 
               
               
                 Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu 
               
               
                                 475                 480                 485 
               
               
                   
               
               
                 GCT AGG CCT GAT CCC AAG AAG AAC CTC ACT ACT TTA GTG AAA GCA 
                 1605 
               
               
                 Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala 
               
               
                                 490                 495                 500 
               
               
                   
               
               
                 TTT GGT GAA TGT CGT CCA TTG AGA GAG CTT GCT AAT CTT ACT TTG 
                 1650 
               
               
                 Phe Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu Thr Leu 
               
               
                                 505                 510                 515 
               
               
                   
               
               
                 ATA ATG GGT AAT CGA GAT AAT ATC GAC GAA ATG TCT AGC ACC AAT 
                 1695 
               
               
                 Ile Met Gly Asn Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn 
               
               
                                 520                 525                 520 
               
               
                   
               
               
                 TCT GCA CTT CTT CTT TCA ATC TTG AAA ATG ATA GAT AAG TAT GAT 
                 1740 
               
               
                 Ser Ala Leu Leu Leu Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp 
               
               
                                 535                 540                 540 
               
               
                   
               
               
                 CTT TAT GGT CAA GTA GCT TAT CCT AAA CAC CAC AAG CAG TCA GAT 
                 1785 
               
               
                 Leu Tyr Gly Gln Val Ala Tyr Pro Lys His His Lys Gln Ser Asp 
               
               
                                 545                 550                 555 
               
               
                   
               
               
                 GTT CCT GAT ATC TAC CGT CTT GCT GCA AAG ACT AAG GGT GTT TTT 
                 1830 
               
               
                 Val Pro Asp Ile Tyr Arg Leu Ala Ala Lys Thr Lys Gly Val Phe 
               
               
                                 560                 565                 570 
               
               
                   
               
               
                 ATT AAT CCA GCT TTT ATT GAG CCT TTT GGA CTG ACT TTG ATT GAG 
                 1875 
               
               
                 Ile Asn Pro Ala Phe Ile Glu Pro Phe Gly Leu Thr Leu Ile Glu 
               
               
                                 575                 580                 585 
               
               
                   
               
               
                 GCA GCA GCT TAT GGT CTC CCA ATG GTA GCC ACA AAA AAT GGA GGA 
                 1920 
               
               
                 Ala Ala Ala Tyr Gly Leu Pro Met Val Ala Thr Lys Asn Gly Gly 
               
               
                                 590                 595                 600 
               
               
                   
               
               
                 CCT GTT GAT ATA CAT AGG GTT CTT GAC AAT GGT CTC TTA GTG GAT 
                 1965 
               
               
                 Pro Val Asp Ile His Arg Val Leu Asp Asn Gly Leu Leu Val Asp 
               
               
                                 605                 610                 615 
               
               
                   
               
               
                 CCC CAT GAT CAG CAG GCA ATT GCT GAT GCT CTT TTG AAG TTG GTT 
                 2010 
               
               
                 Pro His Asp Gln Gln Ala Ile Ala Asp Ala Leu Leu Lys Leu Val 
               
               
                                 620                 625                 630 
               
               
                   
               
               
                 GCT GAT AAG CAA CTG TGG GCT AAA TGC AGG GCA AAT GGA TTA AAA 
                 2055 
               
               
                 Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg Ala Asn Gly Leu Lys 
               
               
                                 635                 640                 645 
               
               
                   
               
               
                 AAT ATC CAC CTT TTC TCA TGG CCC GAG CAC TGT AAA ACT TAT CTA 
                 2100 
               
               
                 Asn Ile His Leu Phe Ser Trp Pro Glu His Cys Lys Thr Tyr Leu 
               
               
                                 650                 655                 660 
               
               
                   
               
               
                 TCC CGG ATA GCT AGC TGC AAA CCA AGG CAA CCA CGC TGG CTG AGA 
                 2145 
               
               
                 Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln Pro Arg Trp Leu Arg 
               
               
                                 665                 670                 675 
               
               
                   
               
               
                 TCC ATT GAT GAT GAT GAT GAA AAT TCA GAA ACA GAT TCA CGT AGT 
                 2190 
               
               
                 Ser Ile Asp Asp Asp Asp Glu Asn Ser Glu Thr Asp Ser Pro Ser 
               
               
                                 680                 685                 690 
               
               
                   
               
               
                 GAT TCC TTG AGA GAT ATT CAT GAT ATA TCT CTG AAT TTG AGA TTT 
                 2235 
               
               
                 Asp Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu Arg Phe 
               
               
                                 695                 700                 705 
               
               
                   
               
               
                 TCA TTA GAT GGG GAA AAG AAT GAC AAT AAA GAA AAT GCT GAT AAT 
                 2280 
               
               
                 Ser Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn 
               
               
                                 710                 715                 720 
               
               
                   
               
               
                 ACA TTA GAC CCC GAA GTT CGA AGG AGC AAG TTA GAG AAT GCT GTT 
                 2325 
               
               
                 Thr Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn Ala Val 
               
               
                                 725                 730                 735 
               
               
                   
               
               
                 TTG TCC TTA TCT AAG GGT GCA CTG AAG AGC ACA TCA AAA TCT TGG 
                 2370 
               
               
                 Leu Ser Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys Ser Trp 
               
               
                                 740                 745                 750 
               
               
                   
               
               
                 TCG TCA GAC AAG GCA GAC CAA AAC CCT GGT GCT GGT AAA TCC CCA 
                 2415 
               
               
                 Ser Ser Asp Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys Phe Pro 
               
               
                                 755                 760                 765 
               
               
                   
               
               
                 GCG ATT AGG AGG AGG CGA CAT ATT TTT GTT ATT GCA GTG GAT TGT 
                 2460 
               
               
                 Ala Ile Arg Arg Arg Arg His Ile Phe Val Ile Ala Val Asp Cys 
               
               
                                 770                 775                 780 
               
               
                   
               
               
                 GAT GCT AGC TCA GGA CTC TCT GGA AGT GTG AAA AAG ATA TTT GAG 
                 2505 
               
               
                 Asp Ala Ser Ser Gly Leu Ser Gly Ser Val Lys Lys Ile Phe Glu 
               
               
                                 785                 790                 795 
               
               
                   
               
               
                 GCT GTA GAG AAG GAA AGG GCA GAG GGT TCC ATT GGA TTT ATC CTG 
                 2550 
               
               
                 Ala Val Glu Lys Glu Arg Ala Glu Gly Ser Ile Gly Phe Ile Leu 
               
               
                                 800                 805                 810 
               
               
                   
               
               
                 GCT ACA TCT TTC AAT ATA TCA GAA GTA CAG TCT TTC CTG CTT TCA 
                 2595 
               
               
                 Ala Thr Ser Phe Asn Ile Ser Glu Val Gln Ser Phe Leu Leu Ser 
               
               
                                 815                 820                 825 
               
               
                   
               
               
                 GAG GGC ATG AAT CCT ACT GAT TTT GAT GCT TAC ATA TGC AAT AGT 
                 2640 
               
               
                 Glu Gly Met Asn Pro Thr Asp Phe Asp Ala Tyr Ile Cys Asn Ser 
               
               
                                 830                 835                 840 
               
               
                   
               
               
                 GGT GGT GAT CTT TAT TAT TCG TCC TTC CAT TCT GAG CAA AAT CCT 
                 2685 
               
               
                 Gly Gly Asp Leu Tyr Tyr Ser Ser Phe His Ser Glu Gln Asn Pro 
               
               
                                 845                 850                 855 
               
               
                   
               
               
                 TTT GTA GTT GAC TTG TAC TAT CAC TCA CAT ATT GAG TAT CGT TGG 
                 2730 
               
               
                 Phe Val Val Asp Leu Tyr Tyr His Ser His Ile Glu Tyr Arg Trp 
               
               
                                 860                 865                 870 
               
               
                   
               
               
                 GGG GGC GAA GGA TTG AGA AAG ACT TTG GTG CGT TGG GCC GCC TCT 
                 2775 
               
               
                 Gly Gly Glu Gly Leu Arg Lys Thr Leu Val Arg Trp Ala Ala Ser 
               
               
                                 875                 880                 885 
               
               
                   
               
               
                 ATC ATT GAT AAG AAT GGT GAA AAT GGA GAT CAC ATT GTT GTT GAG 
                 2820 
               
               
                 Ile Ile Asp Lys Asn Gly Glu Asn Gly Asp His Ile Val Val Glu 
               
               
                                 890                 895                 900 
               
               
                   
               
               
                 GAT GAA GAC AAT TCA GCT GAC TAC TGC TAT ACT TTC AAA GTG TGC 
                 2865 
               
               
                 Asp Glu Asp Asn Ser Ala Asp Tyr Cys Tyr Thr Phe Lys Val Cys 
               
               
                                 905                 910                 915 
               
               
                   
               
               
                 AAG CCT GGG AGG GTT GCT CCA TGT AAA GAG GTT AGA AAA GTA ATG 
                 2910 
               
               
                 Lys Pro Gly Thr Val Pro Pro Ser Lys Glu Leu Arg Lys Val Met 
               
               
                                 920                 925                 930 
               
               
                   
               
               
                 CGA ATT CAG GCA GTT CGT TGT CAC GCT GTT TAT TGT CAA AAT GGG 
                 2955 
               
               
                 Arg Ile Gln Ala Leu Arg Cys His Ala Val Tyr Cys Gln Asn Gly 
               
               
                                 935                 940                 945 
               
               
                   
               
               
                 AGT AGG ATT AAT GTG ATC CCT GTA CTG GCA TCT CGG TCC CAA GCA 
                 3000 
               
               
                 Ser Arg Ile Asn Val Ile Pro Val Leu Ala Ser Arg Ser Gln Ala 
               
               
                                 950                 955                 960 
               
               
                   
               
               
                 CTC AGG TAC TTA TAT CTG CGA TGG GGA ATG GAC TTG TCG AAG TTG 
                 3045 
               
               
                 Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu 
               
               
                                 965                 970                 975 
               
               
                   
               
               
                 GTG GTT TTC GTC GGA GAA AGT GGT GAT ACC GAT TAT GAA GGA TTA 
                 3090 
               
               
                 Val Val Phe Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu 
               
               
                                 980                 985                 990 
               
               
                   
               
               
                 ATG GGT GGT CTA CGC AAG GCT GTC ATA ATG AAA GGC CTC TGC ACT 
                 3135 
               
               
                 Ile Gly Gly Leu Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr 
               
               
                                 995                 1000                1005 
               
               
                   
               
               
                 AAT GCA AGC AGC TTA ATT CAC GGT AAT AGG AAT TAC CCG CTA TCT 
                 3180 
               
               
                 Asn Ala Ser Ser Leu Ile His Gly Asn Arg Asn Tyr Pro Leu Ser 
               
               
                                 1010                1015                1020 
               
               
                   
               
               
                 GAT GTT TTA CCA TTC GAC AGC CCT AAT GTC ATC CAA GCG GAC GAG 
                 3225 
               
               
                 Asp Val Leu Pro Phe Asp Ser Pro Asn Val Ile Gln Ala Asp Glu 
               
               
                                 1025                1030                1035 
               
               
                   
               
               
                 GAA TGT AGC AGC ACC GAA ATC CGT TGC TTA CTG GAG AAA CTA GCG 
                 3270 
               
               
                 Glu Cys Ser Ser Thr Glu Ile Arg Cys Leu Leu Glu Lys Leu Ala 
               
               
                                 1040                1045                1050 
               
               
                   
               
               
                 GTA CTC AAA GGA TAA TACCCTTCCC CCTTTGATTG TCAAAAACCT 
                 3315 
               
               
                   
               
               
                 Val Leu Lys Gly End 
               
               
                             1054 
               
               
                   
               
               
                 ATATGAGCTA TAAGACTATG GCATGAAAAG AATGGCCATC CATTTGGCTT GTCTTTTGAA 
                 3375 
               
               
                   
               
               
                 GCTGTTAATA CTTTTCAACA GACTACAAAA TGAGATGAGT CCTTTGATCC TCTTTAAAGG 
                 3435 
               
               
                   
               
               
                 ACATAAAAGC TTTATGCAAG AACCAGTGCT GTAAAGTTAT AGAATTTCTT TTGCTATATA 
                 3495 
               
               
                   
               
               
                 TGACATTCGA CAGAACCAGT TCCGGTTCAT CGAGAAAAAG AAATAAATTT CAACTTATAA 
                 3555 
               
               
                   
               
               
                 ACATGCCTGA TCATGTAAAT TATCATATAC ATCCATCGGA AGGCATTATC GATGGGTTAT 
                 3615 
               
               
                   
               
               
                 CAGATTTTTT 
                 3625 
               
               
                   
               
               
                 SPS 3 sequence (Seq. ID No.5 and No.6) 
               
               
                   
               
               
                    ATTTTTT TCTCTAAATT CTCTCTCACT GTCCTTATCA TTTCACCACC TCCATAAATC 
                 57 
               
               
                   
               
               
                 TAGAAACATC TTTTCTATTC CGTTAATCTC TGTAGCACAC GGCGGAGTGC GGCGGAGGAG 
                 117 
               
               
                   
               
               
                 ATG GCG GGA AAC GAC TGG ATT AAC AGT TAG TTA GAG GCG ATA CTG 
                 162 
               
               
                 Met Ala Gly Asn Asp Trp Ile Asn Ser Tyr Leu Glu Ala Ile Leu 
               
               
                 1               5                   10                  15 
               
               
                   
               
               
                 GAT GTA GGA CCA GGG CTA GAT GAT AAG AAA TCA TCG TTG TTG TTG 
                 207 
               
               
                 Asp Val Gly Pro Gly Leu Asp Asp Lys Lys Ser Ser Leu Leu Leu 
               
               
                                 20                  25                  30 
               
               
                   
               
               
                 AGA GAA AGA GGG AGG TTT AGT CCG ACG AGG TAC TTT GTT GAG GAA 
                 252 
               
               
                 Arg Glu Arg Gly Arg Phe Ser Pro Thr Arg Tyr The Val Glu Glu 
               
               
                                 25                  40                  45 
               
               
                   
               
               
                 GTT ATT ACT GGA TTC GAT GAG ACT GAT TTG CAT CGC TCG TGG ATC 
                 297 
               
               
                 Val Ile Thr Gly Phe Asp Glu Thr Asp Leu His Arg Ser Trp Ile 
               
               
                                 50                  55                  60 
               
               
                   
               
               
                 CGA GCA CAA GCT ACT CGG AGT CCG CAG GAG AGG AAT ACT AGG CTC 
                 342 
               
               
                 Arg Ala Gln Ala Thr Arg Ser Pro Gln Glu Arg Asn Thr Arg Leu 
               
               
                                 65                  70                  75 
               
               
                   
               
               
                 GAG AAT ATG TGC TGG AGG ATT TGG AAT TTG GCT CGC CAG AAA AAG 
                 387 
               
               
                 Glu Asn Met Cys Trp Arg Ile Trp Asn Leu Ala Arg Gln Lys Lys 
               
               
                                 80                  85                  90 
               
               
                   
               
               
                 CAG CTT GAG GGA GAG CAA GCT CAG TGG ATG GCA AAA CGC CGT CAA 
                 432 
               
               
                 Gln Leu Glu Gly Glu Gln Ala Gln Trp Met Ala Lys Arg Arg Gln 
               
               
                                 95                  100                 105 
               
               
                   
               
               
                 GAA CGT GAG AGA GGT CGC AGA GAA GCA GTT GCT GAT ATG TCA GAG 
                 477 
               
               
                 Glu Arg Glu Arg Gly Arg Arg Glu Ala Val Ala Asp Met Ser Glu 
               
               
                                 110                 115                 120 
               
               
                   
               
               
                 GAT CTA TCT GAG GGA GAG AAA GGA GAT ATA GTC GCT GAC ATG TCA 
                 522 
               
               
                 Asp Leu Ser Glu Gly Glu Lys Gly Asp Ile Val Ala Asp Met Ser 
               
               
                                 125                 130                 135 
               
               
                   
               
               
                 TCT CAT GGT GAA AGT ACC AGA GGC CGA TTG CCT AGA ATC AGT TCT 
                 567 
               
               
                 Ser His Gly Glu Ser Thr Arg Gly Arg Leu Pro Arg Ile Ser Ser 
               
               
                                 140                 145                 150 
               
               
                   
               
               
                 GTT GAG ACA ATG GAA GCA TGG GTC AGT CAG CAG AGA GGA AAG AAG 
                 612 
               
               
                 Val Glu Thr Met Glu Ala Trp Val Ser Gln Gln Arg Gly Lys Lys 
               
               
                                 155                 160                 165 
               
               
                   
               
               
                 CTT TAT ATC GTG CTT ATA AGT TTA CAT GGT TTA ATT CGG GGT GAG 
                 657 
               
               
                 Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu Ile Arg Gly Glu 
               
               
                                 170                 175                 180 
               
               
                   
               
               
                 AAT ATG GAG CTT GGA CGG GAT TCT GAT ACT GGT GGT CAG GTG AAG 
                 702 
               
               
                 Asn Met Glu Leu Gly Arg Asp Ser Asp Thr Gly Gly Gln Val Lys 
               
               
                                 185                 190                 195 
               
               
                   
               
               
                 TAT GTA GTT GGA GCA ACT GTT GCA CAA GGT CGT TTG TCA AAG GAT 
                 747 
               
               
                 Tyr Val Val Gly Ala Thr Val Ala Gln Gly Arg Leu Ser Lys Asp 
               
               
                                 200                 205                 210 
               
               
                   
               
               
                 GAA ATA AAC TCA ACC TAC AAG ATA ATG CGG AGA ATA GAG GGT GAA 
                 792 
               
               
                 Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala Glu 
               
               
                                 215                 220                 225 
               
               
                   
               
               
                 GAA TTA ACT CTT GAT GCT TCC GAA ATT GTC ATC ACT AGT ACA AGA 
                 837 
               
               
                 Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser Thr Arg 
               
               
                                 230                 235                 240 
               
               
                   
               
               
                 CAG GAG ATT GAC GAG CAA TGG CGT TTG TAT GAT GGG TTT GAT CCA 
                 882 
               
               
                 Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp Pro 
               
               
                                 245                 250                 255 
               
               
                   
               
               
                 ATA TTA GAG CGT AAG TTA CGT GCA AGG ATC AAG CGC AAT GTC AGC 
                 927 
               
               
                 Ile Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser 
               
               
                                 260                 265                 270 
               
               
                   
               
               
                 TGT TAT GGC AGG TTT ATG CCT CGT ATG GCT GTA ATT CCT CCT GGG 
                 972 
               
               
                 Cys Tyr Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly 
               
               
                                 275                 280                 285 
               
               
                   
               
               
                 ATG GAG TTC CAC CAT ATT GTG CCA CAT GAA GGT GAC ATG GAT GGT 
                 1017 
               
               
                 Met Glu Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly 
               
               
                                 290                 295                 300 
               
               
                   
               
               
                 GAA ACA GAA GGA AGT GAA GAT GGA AAG ACC CCG GAT CCA CCT ATT 
                 1062 
               
               
                 Glu Thr Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile 
               
               
                                 305                 310                 315 
               
               
                   
               
               
                 TGG GCA GAG ATT ATG CGC TTC TTT TCT AAT CCA AGG AAG CCT ATG 
                 1107 
               
               
                 Trp Ala Glu Ile Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met 
               
               
                                 320                 330                 335 
               
               
                   
               
               
                 ATA CTC GCA CTT GCT AGG CCT GAT CCC AAG AAG AAC CTC ACT ACT 
                 1152 
               
               
                 Ile Leu Ala Leu Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr 
               
               
                                 340                 345                 350 
               
               
                   
               
               
                 TTA GTG AAA GCA TTT GGT GAA TGT CGT CCA TTG AGA GAC CTT GCT 
                 1197 
               
               
                 Leu Val Lys Ala Phe Gly Glu Cys Arg Pro Leu Arg Asp Leu Ala 
               
               
                                 355                 360                 365 
               
               
                   
               
               
                 AAT CTT ACT TTG ATA ATG GGT AAT CGA GAT AAT ATC GAC GAA ATG 
                 1242 
               
               
                 Asn Leu Thr Leu Ile Met Gly Asn Arg Asp Asn Ile Asp Glu Met 
               
               
                                 370                 375                 380 
               
               
                   
               
               
                 TCT AGC ACC AAT TCT GCA CTT CTT CTT TCA ATC TTC AAG ATG ATA 
                 1287 
               
               
                 Ser Ser Thr Asn Ser Ala Leu Leu Leu Ser Ile Leu Lys Met Ile 
               
               
                                 385                 390                 395 
               
               
                   
               
               
                 GAT AAG TAT GAT CTT TAT GGT CTA GTA GCT TAT CCT AAA CAC CAC 
                 1332 
               
               
                 Asp Lys Tyr Asp Leu Tyr Gly Leu Val Ala Tyr Pro Lys His His 
               
               
                                 400                 405                 410 
               
               
                   
               
               
                 AAG CAG TCA GAT GTT CCT GAT ATC TAC CGT CTT GCT GCA AAG ACT 
                 1377 
               
               
                 Lys Gln Ser Asp Val Pro Asp Ile Tyr Arg Leu Ala Ala Lys Thr 
               
               
                                 415                 420                 425 
               
               
                   
               
               
                 AAG GGT GTT TTT ATT AAT CCA GCT TTT ATT GAG CCT TTT GGA CTG 
                 1422 
               
               
                 Lys Gly Val Phe Ile Asn Pro Ala Phe Ile Glu Pro Phe Gly Leu 
               
               
                                 430                 435                 440 
               
               
                   
               
               
                 ACT TTG ATT GAG GCA GCA GCT TAT GGT CTC CCA ATG GTA GCC ACA 
                 1467 
               
               
                 Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro Met Val Ala Thr 
               
               
                                 445                 450                 455 
               
               
                   
               
               
                 AAA AAT GGA GGA CCT GTT CAT ATA GAT AGG GTT CTT GAC AAT GGT 
                 1512 
               
               
                 Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu Asp Asn Gly 
               
               
                                 460                 465                 470 
               
               
                   
               
               
                 CTC TTA GTG GAT CCC CAT GAT CAG CAG GCA ATT GCT GAT GCT CTT 
                 1557 
               
               
                 Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp Ala Leu 
               
               
                                 475                 480                 485 
               
               
                   
               
               
                 TTG AAG TTG GTT GCT GAT AAG CAA CTG TGG GCT AAA TGC AGG GCA 
                 1602 
               
               
                 Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg Ala 
               
               
                                 490                 495                 500 
               
               
                   
               
               
                 AAT GGA TTA AAA AAT ATG CAC CTT TTC TCA TGG CCC GAG CAC TGT 
                 1647 
               
               
                 Asn Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys 
               
               
                                 505                 510                 515 
               
               
                   
               
               
                 AAA ACT TAT CTA TCC CGG ATA GCT AGC TGC AAA CCG AGG CAA CAT 
                 1692 
               
               
                 Lys Thr Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln His 
               
               
                                 520                 525                 530 
               
               
                   
               
               
                 TCC TTG AGA GAT ATT CAT GAT ATA TCT CTG AAT TTG AGA TTT TCA 
                 1737 
               
               
                 Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu Arg Phe Ser 
               
               
                                 535                 540                 540 
               
               
                   
               
               
                 TTA GAT GGG GAA AAG AAT GAC AAT AAA GAA AAT GCT GAT AAT ACA 
                 1782 
               
               
                 Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn Thr 
               
               
                                 545                 550                 555 
               
               
                   
               
               
                 TTA GAC CCC GAA GTT CGA AGG AGC AAG TTA GAG AAT GCT GTT TTG 
                 1827 
               
               
                 Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn Ala Val Leu 
               
               
                                 560                 565                 570 
               
               
                   
               
               
                 TCC TTA TCT AAG GGT GCA CTG AAG AGC ACA TCA AAA TCT TGG TCG 
                 1872 
               
               
                 Ser Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys Ser Trp Ser 
               
               
                                 575                 580                 585 
               
               
                   
               
               
                 TCA GAC AAG GCA GAC CAA AAT CCT GGT GCT GGT AAA TTC CCA GCG 
                 1917 
               
               
                 Ser Asp Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys Phe Pro Ala 
               
               
                                 590                 595                 600 
               
               
                   
               
               
                 ATT AGG AGG AGG CGA CAT ATT TTT GTT ATT GCA GTG GAT TGT GAT 
                 1962 
               
               
                 Ile Arg Arg Arg Arg His Ile Phe Val Ile Ala Val Asp Cys Asp 
               
               
                                 605                 610                 615 
               
               
                   
               
               
                 GCT AGC TCA GGA CTC TCT GGA AGT ATG AAA AAG ATA TTT GAG GCT 
                 2007 
               
               
                 Ala Ser Ser Gly Leu Ser Gly Ser Met Lys Lys Ile Phe Glu Ala 
               
               
                                 620                 625                 630 
               
               
                   
               
               
                 GTA GAG AAG GAA AGG GCA GAG GGT TCC ATT GGA TTT ATC CTT GCT 
                 2052 
               
               
                 Val Glu Lys Glu Arg Ala Glu Gly Ser Ile Gly Phe Ile Leu Ala 
               
               
                                 635                 640                 645 
               
               
                   
               
               
                 ACA TCT TTC AAT ATA TCA GAA GTA CAG TCT TTC CTG CTT TCA GAG 
                 2097 
               
               
                 Thr Ser Phe Asn Ile Ser Glu Val Glu Ser Phe Leu Leu Ser Glu 
               
               
                                 650                 655                 660 
               
               
                   
               
               
                 GGC ATG AAT CCT ACT GAG CAA AAT CCT TTT GTA GTT GAC TTG TAC 
                 2142 
               
               
                 Gly Met Asn Pro Thr Glu Gln Asn Pro Phe Val Val Asp Leu Tyr 
               
               
                                 665                 670                 675 
               
               
                   
               
               
                 TAT CAC TCA CAT ATT GAG TAT CGT TGG GGG GGC GAA GGG TTG AGA 
                 2187 
               
               
                 Tyr His Ser His Ile Glu Tyr Arg Trp Gly Gly Glu Gly Leu Arg 
               
               
                                 680                 685                 690 
               
               
                   
               
               
                 AAG ACT TTG GTG CGT TGG GCC GCC TCT ATC ATT GAT AAG AAT GGT 
                 2232 
               
               
                 Lys Thr Leu Val Arg Trp Ala Ala Ser Ile Ile Asp Lys Asn Gly 
               
               
                                 695                 700                 705 
               
               
                   
               
               
                 GAA AAT GGA GAT CAC ATT GTT GTT GAG GAT GAA GAC AAT TCA GCT 
                 2277 
               
               
                 Glu Asn Gly Asp His Ile Val Val Glu Asp Glu Asp Asn Ser Ala 
               
               
                                 710                 715                 720 
               
               
                   
               
               
                 GAC TAC TGC TAT ACA TTC AAA GTT TGC AAG CCT GGG ACG GTT CCT 
                 2322 
               
               
                 Asp Tyr Cys Tyr Thr Phe Lys Val Cys Lys Pro Gly Thr Val Pro 
               
               
                                 725                 730                 725 
               
               
                   
               
               
                 CCA TCT AAA GAA CTT AGA AAA GTA ATG CGA ATT CAG GCA CTT CGT 
                 2367 
               
               
                 Pro Ser Lys Glu Leu Arg Lys Val Met Arg Ile Gln Ala Leu Arg 
               
               
                                 740                 745                 750 
               
               
                   
               
               
                 TGT CAC GCT GTT TAT TGT CAA AAT GGG AGT AGG ATT AAT GTG ATC 
                 2412 
               
               
                 Cys His Ala Val Tyr Cys Gln Asn Gly Ser Arg Ile Asn Val Ile 
               
               
                                 755                 760                 765 
               
               
                   
               
               
                 CCT GTA CTG GCA TCT CGG TCC CAA GCA CTC AGG TAC TTA TAT CTG 
                 2457 
               
               
                 Pro Val Leu Ala Ser Arg Ser Gln Ala Leu Arg Tyr Leu Tyr Leu 
               
               
                                 770                 775                 780 
               
               
                   
               
               
                 CGA TGG GGA ATG GTC CCT GTA CTG GCA TCT CGG TCC CAA GCA CTC 
                 2502 
               
               
                 Arg Trp Gly Met Val Pro Val Leu Ala Ser Arg Ser Gln Ala Leu 
               
               
                                 785                 790                 795 
               
               
                   
               
               
                 AGG TAC TTA TAT CTG CGA TGG GGA ATG GTC CCT GTA CTG GCA TCT 
                 2547 
               
               
                 Arg Tyr Leu Tyr Leu Arg Trp Gly Met Val Pro Val Leu Ala Ser 
               
               
                                 800                 805                 810 
               
               
                   
               
               
                 CGG TCC CAA GCA CTC AGG TAC TTA TAT GTG CGA TGG GGA ATG GAC 
                 2592 
               
               
                 Arg Ser Gln Ala Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp 
               
               
                                 815                 820                 825 
               
               
                   
               
               
                 TTG TCG AAG TTG GTG GTT TTC GTC GGA GAA AGT GGT GAT ACC GAT 
                 2637 
               
               
                 Leu Ser Lys Leu Val Val Phe Val Gly Glu Ser Gly Asp Thr Asp 
               
               
                                 830                 835                 840 
               
               
                   
               
               
                 TAT GAA GGA TTG ATC GGT GGT CTA CGC AAG GCT GTC ATA ATG AAA 
                 2682 
               
               
                 Tyr Glu Gly Leu Ile Gly Gly Leu Arg Lys Ala Val Ile Met Lys 
               
               
                                 845                 850                 855 
               
               
                   
               
               
                 GGA CTC TGC ACT AAT GCA AGC AGC TTA ATT CAC GGT AAT AGG AAT 
                 2727 
               
               
                 Gly Leu Cys Thr Asn Ala Ser Ser Leu Ile His Gly Asn Arg Asn 
               
               
                                 860                 865                 870 
               
               
                   
               
               
                 TAC CCG CTA TCT GAT GTT TTA CCA TTC GAG AGC CCT AAT GTC ATC 
                 2772 
               
               
                 Tyr Pro Leu Ser Asp Val Leu Pro Phe Glu Ser Pro Asn Val Ile 
               
               
                                 875                 880                 885 
               
               
                   
               
               
                 CAA GCG GAT GAG GAA TGT AGC AGC ACC GGA ATC CGT TCC TTA CTG 
                 2817 
               
               
                 Gln Ala Asp Glu Glu Cys Ser Ser Thr Gly Ile Arg Ser Leu Leu 
               
               
                                 905                 910                 915 
               
               
                   
               
               
                 GAG AAA CTA GCG GTA CTC AAA GGA TAA TACCCTTCCC CCTTTGATTG 
                 2864 
               
               
                 Glu Lys Leu Ala Val Leu Lys Gly End 
               
               
                                 920 
               
               
                   
               
               
                 TCAAAAACCT ATATGAGCTA AGATTATGCC ATGAAAAGAA TGGCCATCCA TTTGGCTTGT 
                 2924 
               
               
                   
               
               
                 CTTTTG 
                 2930 
               
             
          
         
       
     
     All sequences are cDNA sequences and stem from a cDNA library of leaf tissue. The expression gene is the same in various plant tissues. As a promoter, there can generally be used any promoter which is active in plants. The promoter should ensure that the foreign gene is expressed in the plant. The promoter can be chosen so that the expression occurs only in specified tissues, at a determined time point in the plant&#39;s development or at a time point determined by outside influences. The promoter can be homologous or heterologous to the plant. Suitable promoters are e.g. the promoter of the 355 RNA of the cauliflower mosaic virus, the patatin promoter B33 (Rocha-Sosa et al. (1989) EMBO J 8: 23-29) or a promoter that ensures expression only in photosynthetically active tissues. Other promoters can be used which ensure expression only in specified organs, such as the root, tuber, seed, stem or specified cell types such as mesophyllic, epidermal or transport cells. For hindering cold sweetening, suitable promoters are those which ensure an activation of the transcription is stored in harvested parts of the plants. For this, there can be considered cold induced promoters or such promoters that become active during the transition of the tuber from the phase where it stores material to the phase where it gives up material. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 provides the structure of the 35S-anti-pot-SPS gene; 
     FIG. 2 provides the structure of the B33-anti-pot-SPS gene; 
     FIG. 3 provides an analysis of plants transformed with a plasmid which included the 35S-anti-pot-SPS gene; and 
     FIG. 4 is an analysis of plants transformed with a plasmid which included the B33-anti-pot-SPS gene. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The coding sequence contains the information for the formation of an mRNA for the sucrose-phosphate-synthase (SPS) or for the formation of an anti-sense RNA for the SPS. Whether the translatable MRNA or an anti-sense RNA is formed depends on the orientation of the coding sequence in relation to the promoter. If the 3′ end of the coding sequence is fused to the 3′ end of the promoter, an anti-sense RNA results, while the fusion of the 5′ end of the coding sequence to the 3′ end of the promoter promoter, a translatable RNA results. This latter leads to an increase of the SPS activity in the cell, while the first leads to a reduction of the SPS activity in the cell. Such a reduction of SPS activity is especially significant in view of the undesirable formation of sucrose and/or reducing sugars as a result of cold storage of harvested organs. 
     The coding sequence for SPS can be one of the three described above or one that is derived by modifications of the sequences described above. A derivation can be carried out, e.g. by current methods of mutagenesis and/or recombination. In particular, changes of SPS sequences that lead to a neutralisation of the plant&#39;s own regulation mechanism are contemplated. 
     The DNA sequences of the invention can also be used for the preparation of derivatives whose gene products are not subjected to the plant&#39;s own activity regulation during a phosphorylation reaction. 
     Further, the sequences can also be used for the preparation of derivatives by targeted and non-targeted mutagenesis. 
     The invention relates further to derivatives of the DNA sequences of the invention that are-obtained by exchange of single bases or by deletion or insertion of base sequences and which code for proteins with a comparable activity to sucrose-phosphate-synthase. 
     The 5′ untranslated area of the sequence Seq. ID No 1, is not part of SPS, but is shown as a cloning artefact. The methionine start codon of the coding region lies in a region in which there is no homology of the amino acid sequence to the other SPS sequences. Since this sequence does not also fully coincide in the homologous region with one of the other sequences, it is recognisable that the sequence Seq. ID No 1 is not a derivative of the sequences Seq. ID No 3 and Seq. ID No 5. 
     The termination sequence provides the correct finishing of the transcription and the attachment of a polyadenyl group to the RNA. This polyadenyl group has an important function in the stabilisation of RNA molecules in the cells. With suitable plasmids which contain the DNA sequences of the invention, plants can be transformed with the object of raising and/or reducing the SPS activity and/or modifying the sucrose concentration. 
     Plasmids that can be used include e.g. p35S-anti-pot-SPS (DSM 7125) and pB33-anti-pot-SPS (DSM 7124). With the gene 35S-anti-pot-SPS, located on the plasmid p355-anti-pot-SPS, the concentration of the mRNA for the SPS protein and the enzymatic activity, for example, can be reduced. With the gene B335-anti-pot-SPS, located on the plasmid pB33-anti-pot-SPS, the concentration of the mRNA for the SPS protein and the enzymatic activity, specifically for potato tubers for example, can be reduced. In a similar way to the SPS sequence (Seq. ID No. 1) located on this plasmid, other SPS sequences, e.g. the sequences Seq. ID No. 3 and Seq. ID No. 5 also be cloned in suitable vectors and for the same purpose. 
     In the plant, the SPS is subjected to an activity control by phosphorylation. This allows the plant to regulate the activity of the enzyme within a fixed frame independent of the amount of the SPS protein. If one of the changes occurring outside the activity of the SPS is to achieved, it is necessary to evade the plant&#39;s own regulation mechanism. Therefore changing the phosphorylation possibilities is an important target for influencing the SPS activity and thus the sucrose content of the plant. 
     It is not known in which position in the SPS protein target directed changes of the coding regions can be achieved which serve the purpose of introducing in the plant SPS activity which is not subject to any of the plant&#39;s own controls. 
     The DNA sequence described here, which contains the coding region for SPS from  Solanum tuberosum , allows the identification of the sites of protein phosphorylation of the SPS. By using standard methods (Sambrook, J., Fritsch, E. F., Maniatis, T. (1989) Molecular Cloning: A laboratory Manual, 2nd. Edn., Cold Spring Harbor Laboratory Press, NY, USA), a localisation of the phosphorylation positions of SPS is possible using the DNA sequences of the invention. These being known, by use of the plasmids with the SPS sequence, a target directed mutagenesis (Sambrook et al, 1989) of the coding region of SPS and/or a non-target directed mutagenesis (Sambrook et al, 1989) and subsequent probing of the desired mutations of the coding region of the SPS can be undertaken. Derivatives of the coding region can be prepared with the help of this plasmid, whose derived proteins are not subjected to the plants own regulation mechanisms. 
     Since the SPS enzyme is regulated by phosphorylation in all tested species, except maize, one can refer to sequence comparisons to identify possible phosphorylation sites. The criterium for this is that a serine residue appears in an acidic medium in the regulated SPS protein, but not however with maize. There are 12 such serine residues in the sequences, Seq. ID No. 3 and Seq ID No. 5. In the sequence Seq ID No. 1, the first of the 12 serine residues is missing, since the coding region begins just later. The sequence, Seq. ID No. 1 is thus especially suitable for the production of SPS activity in plants that is not liable to endogenous activity regulation. 
     For the introduction of the SPS sequence in higher plants, a large number of cloning vectors are available which contain a replication signal for  E. coli  and a marker, which allows for the selection of the transformed cells. Examples of vectors are pBR 322, pUC-series, M13 mp-series, PACYC 184; EMBL 3 etc. According to the introduction method of the desired gene in the plant, other DNA sequences may be suitable. Should the Ti- or Ri-plasmid be used, e.g. for the transformation of the plant cell, then at least the right boundary, often however both the right and left boundary of the Ti- and Ri-Plasmid T-DNA, is attached, as a flanking region, to the gene being introduced. The use of T-DNA for the transformation of plants cells has been intensively researched and is well described in EP 120 516; Hoekama, In: The Binary Plant Vector System, Offset-drukkerij Kanters B. V. Alblasserdam, (1985), Chapter V; Fraley, et al., Crit. Rev. Plant Sci., 4:1-46 and An et al. (1985) EMBO J. 4: 277-287. Once the introduced DNA is integrated in the genome, it is generally stable there and remains also in the offspring of the original transformed cells. It normally contains a selection marker, which induces resistance in the transformed plant cells against a biocide or antibiotic such as kanamycin, G 418, bleomycin, hygromycin or phosphinotricin etc. The individual marker employed should therefore allow the selection of transformed cells from cells which lack the introduced DNA. 
     For the introduction of DNA into a plant, besides transformation using Agrobacteria, there are many other techniques available. These techniques include the fusion of protoplasts, microinjection of DNA and electroporation, as well as ballistic methods and virus infection. From the transformed plant material, whole plants can be regenerated in a suitable medium which contains antibiotics or biocides for selection. The resulting plants can then betested for the presence of introduced DNA. No special demands are placed on the plasmids in injection and electroporation. Simple plasmids, such as e.g. pUC-derivatives can be used. Should however whole plants be regenerated from such transformed cells the presence of a selectable marker gene is necessary. The transformed cells grow within the plants in the usual manner (see also McCormick et al.(1986) Plant Cell Reports 5: 81-84). These plants can be grown normally and crossed with plants, that possess the same transformed genes or different genes. The resulting hybrid individuals have the corresponding phenotypical properties. 
     Deposits 
     The following plasmids were deposited at the Deutschen Sammlung von Mikroorganismen (DSM) in Braunschweig, Germany on the 12.06.1992 (deposit number): 
     Plasmid p35S-anti-pot-SPS (DSM 7125) Plasmid pB33-anti-pot-SPS (DSM 7124) 
     DETAILED DESCRIPTIONS OF THE DRAWINGS 
     FIG.  1 : Structure of the 35S-anti-pot-SPS gene 
     A=Fragment A: CaMV 35S promoter, nt 6909-7437 (Franck et al.,1980, Cell 21: 285-294) 
     B=Fragment B: sucrose phosphate synthase, EcORV Fragment (nt 1 bis 2011), ca. 2000 bp, orientation: anti-sense 
     C=Fragment C: nt 11748-11939 of the T-DNA of the Ti-plasmid pTiACH5; Gielen et al., 1984, EMBO J 3: 835-846) 
     FIG.  2 : Structure of the B33-anti-pot-SPS gene 
     A=Fragment A: B33 promoter of the patatin gene from  S. tuberosum , (Rocha-Sosa et al., 1989, EMBO J 8: 23-29), ca 530 bp 
     B=Fragment B: sucrose phosphate synthase (s. FIG.  2 ), EcoRV fragment (nt 2011 bis 1), ca. 2000 bp, orientation: anti-sense 
     C=Fragment C: nt 11748-11939 of T-DNA of the Ti-plasmid pTiACH5 (Gielen et al., 1984, EMBO J 3: 835-846) 
     FIG.  3 : shows the results of the transformation of transgenic potato plants. 
     Control=wild type plants 
     1-75=individual transgenic plants 
     FIG.  4 : shows the results of the transformation of potato plants 
     Control=wild type plants 
     3-20=individual transgenic plants 
     In order to understand the examples forming the basis of this invention all the processes necessary for these tests and which are known per se will first of all be listed: 
     1. Cloning Process 
     The vectors pUC 18/19 and M13mp10 series (Yanisch-Perron et al. (1985) Gene 33: 103-119), as well as the vector EMBL 3 (Frischauf et al. (1983) J Mol Biol 170: 827-842) were used for cloning. 
     For the plant transformations, the gene constructs were cloned in the binary vector BIN 19 (Bevan (1984) Nucl. Acids Res 12: 8711-8720) 
     2. Bacterial Strains 
     The  E. coli  strain BMH71-18 (Messing et al., Proc. Natl. Acad. Sci. USA (1977), 24, 6342-6346) or TB1 was used for the pUC and M13 mP vectors. 
     For the vector BIN19, only the  E. coli  strain TB1 was used. TB1 is a recombinant-negative, tetracycline-resistant derivative of strain JM101 (Yanisch-Perron et al., Gene (1985), 33, 103-119). The genotype of the TB1 strain is (Bart Barrel, personal communication): F′ (traD36, proAB, lacI, lacZΔM15), Δ (lac, pro), SupE, this, recA, Sr1::Tn10(TcR). 
     The transformation of the plasmids into the potato plants was carried out using  Agrobacterium tumefaciens  strain LBA4404 (Bevan, (1984), Nucl. Acids Res. 12, 8711-8720). 
     3. Transformation of  Agrobacterium tumefaciens    
     In the case of BIN19 derivatives, the insertion of the DNA into the Agrobacterium was effected by direct transformation in accordance with the method of Holsters et al., (1978) (Mol Gene Genet 163: 181-187). The plasmid DNA of the transformed Agrobacterium was isolated in accordance with the method of Birnboim and Doly (1979) (Nucl Acids Res 7: 1513-1523) and was analysed by gel electrophoresis after suitable restriction cleavage. 
     4. Plant Transformation 
     Ten small leaves, wounded with a scalpel, of a sterile potato culture were placed in 10 ml of MS medium with 2% sucrose containing 30-50 μl of an  Agrobacterium tumefaciens  overnight culture grown under selection. After 3-5 minutes gentle shaking, the leaves were laid out on MS medium of 1.6% glucose, 2 mg/l of zeatin ribose, 0.02 mg/l of naphthylacetic acid, 0.02 mg/l of gibberellic acid, 500 mg/l of claforan, 50 mg/l of kanamycin and 0.8% bacto agar. After incubation for one week at 25° C. and 3000 lux, the claforan concentration in the medium was reduced by half. 
     5. SPS Activity Test 
     The SPS activity was determined according to the method of Siegel and Stitt (1990, Plant Science 66: 205-210) in a two stage analysis. To 180 μl of a solution of 50 mM HEPES/KOH (pH 7.4), 5 mM magnesium chloride, 5 mM fructose-6-phosphate, 25 mM glucose-6-phosphate and 6 mM uridine-5′-diphosphoglucose 20 μl of probe was added and incubated for 10 minutes at 25° C. It was heated for 3 minutes at 95°C., to complete the reaction. After centrifuging, the supernatant was spectroscopically analysed for the liberation of uridine-5′-diphosphate, whereby a pyruvate-kinase coupling enzyme reaction was used. Preparations without hexose phosphate, as well as the measurement of the recovery of added uridine-5′-diphosphate act as controls. 
     EXAMPLES 
     Example 1 
     Clonina of genes of the sucrose-phosphate-synthase from potato 
     Poly-A+ RNA was isolated from large leaves of spinach plants as well as potato plants grown in the greenhouse. Resulting from the poly-A+ RNA, a CDNA library in the expression vector Lambda Zap II was laid out. 100,000 plaques of both libraries were separated from spinach using a rabbit antiserum directed against pure SPS protein in relation to immunologically cross reacting protein. (Sonnewald et al., 1992, in press). From the potato library, positively reacting clones were obtained. These clones were further purified by standard methods and, by in vivo excision, plasmids were obtained which carried a double stranded cDNA as an insertion. After testing the size of the insertions, individual clones were analysed by determining the primary sequence. 
     Example 2 
     Determination of the Nucleotide Seauence of the SPS from Potato Codina cDNA Molecules and Derivation of the Corresponding Amino acid Sequences 
     The nucleotide sequences of the insertions obtained from Example 1 were determined by standard methods by means of the dideoxy method (Sanger et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467). The nucleotide sequences (Seq. ID No. 1 to Sea. ID No. 3) are described above. The amino acid sequences derived therefrom are also given. 
     Example 3 
     Construct of the Plasmid p35s-anti-pot-SPS and Insertion of Gene 35s-anti-pot-SPS in the Genome of Potato Plants 
     The gene 35s-anti-pot-SPS consists of the three fragments A, B and C (see FIG.  1 ). 
     The plasmid was prepared as follows: 
     From the pbluescript plasmid with the total insertion, an approximately 2 kb size fragment was prepared by EcoRV cleavage, and this was cloned in the SmaI cleavage site of the vector pBinAR (Höfgen &amp; Willmitzer, 1990, Plant Sci., 66-221-230). The vector pBinAR is a derivative of the binary vector BIN 19 (Bevan, 1984, Nucl. Acids Res. 12: 8711-8721-8721) and was transferred using an  Agrobacterium tumefaciens  mediated transformation into potato. Intact, fertile plants were regenerated from the transformed cells. 
     As a result of the transformation, some transgenic potato plants were shown to have a reduced amount of RNA coding for the potato SPS (see FIG.  3 ). 50 μg total RNA in a Northern blot experiment was hybridised with the probe for SPS from potato. 
     Further the plants showed a reduction in SPS activity (see Table I). 
     Thus, by the transfer and expression of the gene 35s-anti-pot-SPS in potato plants, the amount of mRNA for the SPS protein which is formed, as well as the existing enzymatic activity can be significantly reduced. 
     Example 4 
     Construct of Plasmid pB33-anti-pot-SpS and Insertion of Gene B33-anti-pot-SPS in the Genome of Potato Plants 
     The gene B33-anti-pot-SPS consists of the three fragments A, B and C (see FIG.  4 ). The plasmid was prepared in an analogous method to that described in Example 3, except that a pBin 19 derivative was used as starting vector, which contains the B33 promoter of the patatin gene from  Solanum tuberosum  (Rocha-Sosa et al., 1989, EMBO J. 8: 23-29) in place of the 35S promoter of pBinAR. 
     The gene B33-anti-pot-SPS was transferred into potato plants using an  Agrobacterium tumefaciens  mediated transformation. Intact, fertile plants were regenerated from the transformed cells. 
     As a result of the transformation, some transgenic potato plants were shown with a reduced amount of RNA coding for the potato SPS (see FIG.  4 ). 50 μg total RNA in a Northern blot experiment was hybridised with the probe for SPS from potato. 
     Further the plants also showed a reduction of the SPS activity only in the tubers. 
     Thus, by the transfer and expression of the gene 35s-anti-pot-SPS in potato plants, the amount of mRNA for the SPS protein which is formed, as well as the existing enzymatic activity can be significantly reduced. 
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE I 
               
             
             
               
                   
               
               
                 Results of the transformation of potato plants 
               
             
          
           
               
                  1 
                 2 
                 3 
                 4 
                 5 
               
               
                   
               
             
          
           
               
                  Control 
                 26.1 
                 3.6 
                 13.8 
                 100 
               
               
                 1-55 
                 11.8 
                 2.7 
                 22.9 
                 45 
               
               
                 1-57 
                 20.4 
                 5.9 
                 28.9 
                 78 
               
               
                 1-59 
                 3.8 
                 1.4 
                 36.8 
                 14.6 
               
               
                 1-67 
                 3.8 
                 1.7 
                 44.7 
                 14.6 
               
               
                 1-69 
                 17.2 
                 2.0 
                 11.7 
                 67 
               
               
                 1-72 
                 14.6 
                 1.9 
                 13.0 
                 56 
               
               
                 1-74 
                 5.1 
                 1.7 
                 33.3 
                 19.5 
               
               
                   
               
               
                 Column 1: Control = Wild type plants, numbers indicate individual transgenic plants  
               
               
                 Column 2: Maximal speed of the enzyme reaction in the SPS activity test in nmol/min/mg.  
               
               
                 Column 3: Speed in the SPS activity test in nmol/min/mg.  
               
               
                 Column 4: Activity level of the SPS in %.  
               
               
                 Column 5: Residual activity of the SPS in %.  
               
             
          
         
       
     
     
       
         
           
             6 
           
           
             1 
             3740 
             DNA 
             Solanum tuberosum 
             
               CDS 
               (957)..(3494) 
             
           
            1
ctattctctc ccctcctttt tctcctctct tcaaccccaa aacttccctt tcaaagcctt     60
tgctttccct ttctcactta cccagatcaa ctaagccaat ttgctgtagc ctcagaaaac    120
agcattccca gattgaaaaa gaatcttttt cagtacccaa aagttgggtt tctcatgtcc    180
agcaaggatt agctgctcta gctatttctt tagcccttaa tttttgtcca gttgtgtctt    240
ctgattctgc attggcatct gaatttgatg tgttaaatga agggccacca aaggactcat    300
atgtagttga tgatgctggt gtgcttagca gggtgacaaa gtctgatttg aaggcattgt    360
tgtctgatgt ggagaagaga aaaggcttcc acattaattt catcactgtc cgcaagctca    420
ctagcaaagc tgatgctttt gagtatgctg accaagtttt ggagaagtgg taccctagtg    480
ttgaacaagg aaatgataag ggtatagttg tgcttgttac aagtcaaaag gaaggcgcaa    540
taaccggtgg ccctgatttt gtaaaggccg ttggagatac tgttcttgat gctaccgtct    600
cagagaacct tccagtgttg gctactgaag agaagtacaa tgaagcagtt ttcagcactg    660
ccacacgtct tgttgcagcc attgatggcc ttcctgatcc tggtggaccc caactcaagg    720
ataacaaaag agagtccaac ttcaaatcca gagaggaaac tgatgagaaa agaggacaat    780
tcacacttgt ggttggtggg ctgttagtga ttgcttttgt tgttcctatg gctcaatact    840
atgcatatgt ttcaaagaag tgaactgtct gattctggaa agttacattt tcgtgagatt    900
tgagtaagca tgtatattat cgtgtacaaa atggtccatt cggaaatgac tgattc atg    959
                                                              Met
                                                                1
aga tat tta aaa agg ata aat atg aag att tgg acc tcc cct aac ata     1007
Arg Tyr Leu Lys Arg Ile Asn Met Lys Ile Trp Thr Ser Pro Asn Ile
              5                  10                  15
acg gat act gcc att tct ttt tca gag atg ctg acg cca ata agt aca     1055
Thr Asp Thr Ala Ile Ser Phe Ser Glu Met Leu Thr Pro Ile Ser Thr
         20                  25                  30
gac ggc ttg atg act gag atg ggg gag agt agt ggt gct tat att att     1103
Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly Ala Tyr Ile Ile
     35                  40                  45
cgc att cct ttt gga cca aga gag aaa tat att cca aaa gaa cag cta     1151
Arg Ile Pro Phe Gly Pro Arg Glu Lys Tyr Ile Pro Lys Glu Gln Leu
 50                  55                  60                  65
tgg ccc tat att ccc gaa ttt gtt gat ggt gca ctt aac cat att att     1199
Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly Ala Leu Asn His Ile Ile
                 70                  75                  80
caa atg tcc aaa gtt ctt ggg gag caa att ggt agt ggc tat cct gtg     1247
Gln Met Ser Lys Val Leu Gly Glu Gln Ile Gly Ser Gly Tyr Pro Val
             85                  90                  95
tgg cct gtt gcc ata cac gga cat tat gct gat gct ggc gac tca gct     1295
Trp Pro Val Ala Ile His Gly His Tyr Ala Asp Ala Gly Asp Ser Ala
        100                 105                 110
gct ctc ctg tca ggt gct tta aat gta cca atg ctt ttc act ggt cac     1343
Ala Leu Leu Ser Gly Ala Leu Asn Val Pro Met Leu Phe Thr Gly His
    115                 120                 125
tca ctt ggt aga gat aag ttg gag caa ctg ttg cga caa ggt cgt ttg     1391
Ser Leu Gly Arg Asp Lys Leu Glu Gln Leu Leu Arg Gln Gly Arg Leu
130                 135                 140                 145
tca aag gat gaa ata aac tca acc tac aag ata atg cgg aga ata gag     1439
Ser Lys Asp Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile Glu
                150                 155                 160
gct gaa gaa tta act ctt gat gct tcc gaa att gtc atc act agt aca     1487
Ala Glu Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser Thr
            165                 170                 175
aga cag gag att gac gag caa tgg cgt ttg tat gat ggg ttt gat cca     1535
Arg Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp Pro
        180                 185                 190
ata tta gag cgt aag tta cgt gca agg atc aag cgc aat gtc agc tgt     1583
Ile Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser Cys
    195                 200                 205
tat ggc agg ttt atg cct cgt atg gct gta att cct cct ggg atg gag     1631
Tyr Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met Glu
210                 215                 220                 225
ttc cac cat att gtg cca cat gaa ggt gac atg gat gga gaa aca gaa     1679
Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr Glu
                230                 235                 240
gga agt gaa gat ggg aag acc ccg gat cca cct att tgg gca gag att     1727
Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu Ile
            245                 250                 255
atg cgc ttc ttt tct aat cca agg aag cct atg ata ctc gca ctt gct     1775
Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu Ala
        260                 265                 270
agg cct gat ccc aag aag aac ctc act act tta gtg aaa gca ttt ggt     1823
Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala Phe Gly
    275                 280                 285
gaa tgt cgt cca ttg aga gag ctt gct aat ctt act ttg ata atg ggt     1871
Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu Thr Leu Ile Met Gly
290                 295                 300                 305
aat cga gat aat atc gac gaa atg tct agc acc aat tct gca ctt ctt     1919
Asn Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn Ser Ala Leu Leu
                310                 315                 320
ctt tca atc ttg aaa atg ata gat aag tat gat ctt tat ggt caa gta     1967
Leu Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp Leu Tyr Gly Gln Val
            325                 330                 335
gct tat cct aaa cac cac aag cag tca gat gtt cct gat atc tac cgt     2015
Ala Tyr Pro Lys His His Lys Gln Ser Asp Val Pro Asp Ile Tyr Arg
        340                 345                 350
ctt gct gca aag act aag ggt gtt ttt att aat cca gct ttt att gag     2063
Leu Ala Ala Lys Thr Lys Gly Val Phe Ile Asn Pro Ala Phe Ile Glu
    355                 360                 365
cct ttt gga ctg act ttg att gag gca gca gct tat ggt ctc cca atg     2111
Pro Phe Gly Leu Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro Met
370                 375                 380                 385
gta gcc aca aaa aat gga gga cct gtt gat ata cat agg gtt ctt gac     2159
Val Ala Thr Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu Asp
                390                 395                 400
aat ggt ctc tta gtg gat ccc cat gat cag cag gca att gct gat gct     2207
Asn Gly Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp Ala
            405                 410                 415
ctt ttg aag ttg gtt gct gat aag caa ctg tgg gct aaa tgc agg gca     2255
Leu Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg Ala
        420                 425                 430
aat gga tta aaa aat atc cac ctt ttc tca tgg ccc gag cac tgt aaa     2303
Asn Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys Lys
    435                 440                 445
act tat cta tcc cgg ata gct agc tgc aaa cca agg caa cca cgc tgg     2351
Thr Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln Pro Arg Trp
450                 455                 460                 465
ctg aga tcc att gat gat gat gat gaa aat tca gaa aca gat tca cct     2399
Leu Arg Ser Ile Asp Asp Asp Asp Glu Asn Ser Glu Thr Asp Ser Pro
                470                 475                 480
agt gat tcc ttg aga gat att cat gat ata tct ctg aat ttg aga ttt     2447
Ser Asp Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu Arg Phe
            485                 490                 495
tca tta gat ggg gaa aag aat gac aat aaa gaa aat gct gat aat aca     2495
Ser Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn Thr
        500                 505                 510
tta gac ccc gaa gtt cga agg agc aag tta gag aat gct gtt ttg tcc     2543
Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn Ala Val Leu Ser
    515                 520                 525
tta tct aag ggt gca ctg aag agc aca tca aaa tct tgg tcg tca gac     2591
Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys Ser Trp Ser Ser Asp
530                 535                 540                 545
aag gca gac caa aac cct ggt gct ggt aaa ttc cca gcg att agg agg     2639
Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys Phe Pro Ala Ile Arg Arg
                550                 555                 560
agg cga cat att ttt gtt att gca gtg gat tgt gat gct agc tca gga     2687
Arg Arg His Ile Phe Val Ile Ala Val Asp Cys Asp Ala Ser Ser Gly
            565                 570                 575
ctc tct gga agt gtg aaa aag ata ttt gag gct gta gag aag gaa agg     2735
Leu Ser Gly Ser Val Lys Lys Ile Phe Glu Ala Val Glu Lys Glu Arg
        580                 585                 590
gca gag ggt tcc att gga ttt atc ctg gct aca tct ttc aat ata tca     2783
Ala Glu Gly Ser Ile Gly Phe Ile Leu Ala Thr Ser Phe Asn Ile Ser
    595                 600                 605
gaa gta cag tct ttc ctg ctt tca gag ggc atg aat cct act gat ttt     2831
Glu Val Gln Ser Phe Leu Leu Ser Glu Gly Met Asn Pro Thr Asp Phe
610                 615                 620                 625
gat gct tac ata tgc aat agt ggt ggt gat ctt tat tat tcg tcc ttc     2879
Asp Ala Tyr Ile Cys Asn Ser Gly Gly Asp Leu Tyr Tyr Ser Ser Phe
                630                 635                 640
cat tct gag caa aat cct ttt gta gtt gac ttg tac tat cac tca cat     2927
His Ser Glu Gln Asn Pro Phe Val Val Asp Leu Tyr Tyr His Ser His
            645                 650                 655
att gag tat cgt tgg ggg ggc gaa gga ttg aga aag act ttg gtg cgt     2975
Ile Glu Tyr Arg Trp Gly Gly Glu Gly Leu Arg Lys Thr Leu Val Arg
        660                 665                 670
tgg gcc gcc tct atc att gat aag aat ggt gaa aat gga gat cac att     3023
Trp Ala Ala Ser Ile Ile Asp Lys Asn Gly Glu Asn Gly Asp His Ile
    675                 680                 685
gtt gtt gag gat gaa gac aat tca gct gac tac tgc tat act ttc aaa     3071
Val Val Glu Asp Glu Asp Asn Ser Ala Asp Tyr Cys Tyr Thr Phe Lys
690                 695                 700                 705
gtc tgc aag cct ggg acg gtt cct cca tct aaa gag ctt aga aaa gta     3119
Val Cys Lys Pro Gly Thr Val Pro Pro Ser Lys Glu Leu Arg Lys Val
                710                 715                 720
atg cga att cag gca ctt cgt tgt cac gct gtt tat tgt caa aat ggg     3167
Met Arg Ile Gln Ala Leu Arg Cys His Ala Val Tyr Cys Gln Asn Gly
            725                 730                 735
agt agg att aat gtg atc cct gta ctg gca tct cgg tcc caa gca ctc     3215
Ser Arg Ile Asn Val Ile Pro Val Leu Ala Ser Arg Ser Gln Ala Leu
        740                 745                 750
agg tac tta tat ctg cga tgg gga atg gac ttg tcg aag ttg gtg gtt     3263
Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu Val Val
    755                 760                 765
ttc gtc gga gaa agt ggt gat acc gat tat gaa gga tta atc ggt ggt     3311
Phe Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu Ile Gly Gly
770                 775                 780                 785
cta cgc aag gct gtc ata atg aaa ggc ctc tgc act aat gca agc agc     3359
Leu Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr Asn Ala Ser Ser
                790                 795                 800
tta att cac ggt aat agg aat tac ccg cta tct gat gtt tta cca ttc     3407
Leu Ile His Gly Asn Arg Asn Tyr Pro Leu Ser Asp Val Leu Pro Phe
            805                 810                 815
gac agc cct aat gtc atc caa gcg gac gag gaa tgt agc agc acc gaa     3455
Asp Ser Pro Asn Val Ile Gln Ala Asp Glu Glu Cys Ser Ser Thr Glu
        820                 825                 830
atc cgt tgc tta ctg gtg aaa cta gcg gta ctc aaa gga taataccctt      3504
Ile Arg Cys Leu Leu Val Lys Leu Ala Val Leu Lys Gly
    835                 840                 845
ccccctttga ttgtcaaaaa cctatatgag ctataagact atgccatgaa aagaatggcc   3564
atccatttgg cttgtctttt gaagctgtta atacttttca acagactaca aaatgagatg   3624
agtcctttga tcctctttaa aggacataaa agctttatgc aagaaccagt gctgtaaagt   3684
tatagaattt cttttgctat atatgacatt cgacagaacc tgttccggtt catcga       3740
 
           
             2 
             846 
             PRT 
             Solanum tuberosum 
           
            2
Met Arg Tyr Leu Lys Arg Ile Asn Met Lys Ile Trp Thr Ser Pro Asn
  1               5                  10                  15
Ile Thr Asp Thr Ala Ile Ser Phe Ser Glu Met Leu Thr Pro Ile Ser
             20                  25                  30
Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly Ala Tyr Ile
         35                  40                  45
Ile Arg Ile Pro Phe Gly Pro Arg Glu Lys Tyr Ile Pro Lys Glu Gln
     50                  55                  60
Leu Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly Ala Leu Asn His Ile
 65                  70                  75                  80
Ile Gln Met Ser Lys Val Leu Gly Glu Gln Ile Gly Ser Gly Tyr Pro
                 85                  90                  95
Val Trp Pro Val Ala Ile His Gly His Tyr Ala Asp Ala Gly Asp Ser
            100                 105                 110
Ala Ala Leu Leu Ser Gly Ala Leu Asn Val Pro Met Leu Phe Thr Gly
        115                 120                 125
His Ser Leu Gly Arg Asp Lys Leu Glu Gln Leu Leu Arg Gln Gly Arg
    130                 135                 140
Leu Ser Lys Asp Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile
145                 150                 155                 160
Glu Ala Glu Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser
                165                 170                 175
Thr Arg Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp
            180                 185                 190
Pro Ile Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser
        195                 200                 205
Cys Tyr Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met
    210                 215                 220
Glu Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr
225                 230                 235                 240
Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu
                245                 250                 255
Ile Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu
            260                 265                 270
Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala Phe
        275                 280                 285
Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu Thr Leu Ile Met
    290                 295                 300
Gly Asn Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn Ser Ala Leu
305                 310                 315                 320
Leu Leu Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp Leu Tyr Gly Gln
                325                 330                 335
Val Ala Tyr Pro Lys His His Lys Gln Ser Asp Val Pro Asp Ile Tyr
            340                 345                 350
Arg Leu Ala Ala Lys Thr Lys Gly Val Phe Ile Asn Pro Ala Phe Ile
        355                 360                 365
Glu Pro Phe Gly Leu Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro
    370                 375                 380
Met Val Ala Thr Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu
385                 390                 395                 400
Asp Asn Gly Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp
                405                 410                 415
Ala Leu Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg
            420                 425                 430
Ala Asn Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys
        435                 440                 445
Lys Thr Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln Pro Arg
    450                 455                 460
Trp Leu Arg Ser Ile Asp Asp Asp Asp Glu Asn Ser Glu Thr Asp Ser
465                 470                 475                 480
Pro Ser Asp Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu Arg
                485                 490                 495
Phe Ser Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn
            500                 505                 510
Thr Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn Ala Val Leu
        515                 520                 525
Ser Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys Ser Trp Ser Ser
    530                 535                 540
Asp Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys Phe Pro Ala Ile Arg
545                 550                 555                 560
Arg Arg Arg His Ile Phe Val Ile Ala Val Asp Cys Asp Ala Ser Ser
                565                 570                 575
Gly Leu Ser Gly Ser Val Lys Lys Ile Phe Glu Ala Val Glu Lys Glu
            580                 585                 590
Arg Ala Glu Gly Ser Ile Gly Phe Ile Leu Ala Thr Ser Phe Asn Ile
        595                 600                 605
Ser Glu Val Gln Ser Phe Leu Leu Ser Glu Gly Met Asn Pro Thr Asp
    610                 615                 620
Phe Asp Ala Tyr Ile Cys Asn Ser Gly Gly Asp Leu Tyr Tyr Ser Ser
625                 630                 635                 640
Phe His Ser Glu Gln Asn Pro Phe Val Val Asp Leu Tyr Tyr His Ser
                645                 650                 655
His Ile Glu Tyr Arg Trp Gly Gly Glu Gly Leu Arg Lys Thr Leu Val
            660                 665                 670
Arg Trp Ala Ala Ser Ile Ile Asp Lys Asn Gly Glu Asn Gly Asp His
        675                 680                 685
Ile Val Val Glu Asp Glu Asp Asn Ser Ala Asp Tyr Cys Tyr Thr Phe
    690                 695                 700
Lys Val Cys Lys Pro Gly Thr Val Pro Pro Ser Lys Glu Leu Arg Lys
705                 710                 715                 720
Val Met Arg Ile Gln Ala Leu Arg Cys His Ala Val Tyr Cys Gln Asn
                725                 730                 735
Gly Ser Arg Ile Asn Val Ile Pro Val Leu Ala Ser Arg Ser Gln Ala
            740                 745                 750
Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu Val
        755                 760                 765
Val Phe Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu Ile Gly
    770                 775                 780
Gly Leu Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr Asn Ala Ser
785                 790                 795                 800
Ser Leu Ile His Gly Asn Arg Asn Tyr Pro Leu Ser Asp Val Leu Pro
                805                 810                 815
Phe Asp Ser Pro Asn Val Ile Gln Ala Asp Glu Glu Cys Ser Ser Thr
            820                 825                 830
Glu Ile Arg Cys Leu Leu Val Lys Leu Ala Val Leu Lys Gly
        835                 840                 845
 
           
             3 
             3625 
             DNA 
             Solanum tuberosum 
             
               CDS 
               (121)..(3282) 
             
           
            3
atttttttct ctaagttctc tctcgctgtc cttatcattt caccacctcc ataaatctag     60
aaacatcttt tctactccgt taatctctct agcacacggc ggaggagtgc ggcggaggag    120
atg gcg gga aac gat tgg att aac agt tac tta gag gcg ata ctg gat      168
Met Ala Gly Asn Asp Trp Ile Asn Ser Tyr Leu Glu Ala Ile Leu Asp
  1               5                  10                  15
gtt gga cca ggg cta gat gat aag aag tca tcg ttg ttg ttg aga gaa      216
Val Gly Pro Gly Leu Asp Asp Lys Lys Ser Ser Leu Leu Leu Arg Glu
             20                  25                  30
aga ggg agg ttt agt ccg acg agg tac ttt gtt gag gaa gtt att act      264
Arg Gly Arg Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu Val Ile Thr
         35                  40                  45
gga ttc gat gag act gat ttg cat cgt tcg tgg atc cga gca caa gct      312
Gly Phe Asp Glu Thr Asp Leu His Arg Ser Trp Ile Arg Ala Gln Ala
     50                  55                  60
act cgg agt ccg cag aga agg aat act agg ctc gag aat atg tgc tgg      360
Thr Arg Ser Pro Gln Arg Arg Asn Thr Arg Leu Glu Asn Met Cys Trp
 65                  70                  75                  80
agg att tgg aat ttg gct cgc cag aaa aag cag ctt gag gga gag caa      408
Arg Ile Trp Asn Leu Ala Arg Gln Lys Lys Gln Leu Glu Gly Glu Gln
                 85                  90                  95
gct cag tgg atg gca aaa cgc cgt caa gaa cgt gaa aga ggt cgc aga      456
Ala Gln Trp Met Ala Lys Arg Arg Gln Glu Arg Glu Arg Gly Arg Arg
            100                 105                 110
gaa gca gtt gct gat atg tca gag gat cta tct gag gga gag aaa gga      504
Glu Ala Val Ala Asp Met Ser Glu Asp Leu Ser Glu Gly Glu Lys Gly
        115                 120                 125
gat ata gtc gct gac atg tca tct cat ggt gaa agt acc aga ggc cga      552
Asp Ile Val Ala Asp Met Ser Ser His Gly Glu Ser Thr Arg Gly Arg
    130                 135                 140
ttg cct aga atc agt tct gtt gag aca atg gaa gca tgg gtc agt cag      600
Leu Pro Arg Ile Ser Ser Val Glu Thr Met Glu Ala Trp Val Ser Gln
145                 150                 155                 160
cag aga gga aag aag ctt tat atc gtg ctt ata agt tta cat ggt tta      648
Gln Arg Gly Lys Lys Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu
                165                 170                 175
att cgg ggt gag aat atg gag ctt gga cgg gat tct gat act ggt ggt      696
Ile Arg Gly Glu Asn Met Glu Leu Gly Arg Asp Ser Asp Thr Gly Gly
            180                 185                 190
cag gtg aag tat gtt gtt gaa ctt gcg agg gcc tta ggg tcg atg cca      744
Gln Val Lys Tyr Val Val Glu Leu Ala Arg Ala Leu Gly Ser Met Pro
        195                 200                 205
ggt gta tat cgg gtt gac ttg ctt act aga caa gta tct tca cca gaa      792
Gly Val Tyr Arg Val Asp Leu Leu Thr Arg Gln Val Ser Ser Pro Glu
    210                 215                 220
gta gat tgg agc tat ggt gag ccg aca gag atg ctg acg cca ata agt      840
Val Asp Trp Ser Tyr Gly Glu Pro Thr Glu Met Leu Thr Pro Ile Ser
225                 230                 235                 240
aca gac ggc ttg atg act gag atg ggg gag agt agt ggt gct tat att      888
Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly Ala Tyr Ile
                245                 250                 255
att cgc att cct ttt gga cca aga gag aaa tat att cca aaa gaa cag      936
Ile Arg Ile Pro Phe Gly Pro Arg Glu Lys Tyr Ile Pro Lys Glu Gln
            260                 265                 270
cta tgg ccc tat att ccc gaa ttt gtt gat ggt gca ctt aac cat att      984
Leu Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly Ala Leu Asn His Ile
        275                 280                 285
att caa atg tcc aaa gtt ctt ggg gag caa att ggt agt ggc tat cct     1032
Ile Gln Met Ser Lys Val Leu Gly Glu Gln Ile Gly Ser Gly Tyr Pro
    290                 295                 300
gtg tgg cct gtt gcc ata cac gga cat tat gct gat gct ggc gac tca     1080
Val Trp Pro Val Ala Ile His Gly His Tyr Ala Asp Ala Gly Asp Ser
305                 310                 315                 320
gct gct ctc ctg tca ggt gct tta aat gta cca atg ctt ttc act ggt     1128
Ala Ala Leu Leu Ser Gly Ala Leu Asn Val Pro Met Leu Phe Thr Gly
                325                 330                 335
cac tca ctt ggt aga gat aag ttg gag caa ctg ttg gca caa ggt cga     1176
His Ser Leu Gly Arg Asp Lys Leu Glu Gln Leu Leu Ala Gln Gly Arg
            340                 345                 350
aag tca aag gat gaa ata aac tca acc tac aag ata atg cgg aga ata     1224
Lys Ser Lys Asp Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile
        355                 360                 365
gag gct gaa gaa tta act ctt gat gct tcc gaa att gtc atc act agt     1272
Glu Ala Glu Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser
    370                 375                 380
aca aga cag gag att gac gag caa tgg cgt ttg tat gat ggg ttt gat     1320
Thr Arg Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp
385                 390                 395                 400
cca ata tta gag cgt aag tta cgt gca agg atc aag cgc aat gtc agc     1368
Pro Ile Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser
                405                 410                 415
tgt tat ggc agg ttt atg cct cgt atg gct gta att cct cct ggg atg     1416
Cys Tyr Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met
            420                 425                 430
gag ttc cac cat att gtg cca cat gaa ggt gac atg gat ggt gaa aca     1464
Glu Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr
        435                 440                 445
gaa gga agt gaa gat ggg aag acc ccg gat cca cct att tgg gca gag     1512
Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu
    450                 455                 460
att atg cgc ttc ttt tct aat cca agg aag cct atg ata ctc gca ctt     1560
Ile Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu
465                 470                 475                 480
gct agg cct gat ccc aag aag aac ctc act act tta gtg aaa gca ttt     1608
Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala Phe
                485                 490                 495
ggt gaa tgt cgt cca ttg aga gag ctt gct aat ctt act ttg ata atg     1656
Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu Thr Leu Ile Met
            500                 505                 510
ggt aat cga gat aat atc gac gaa atg tct agc acc aat tct gca ctt     1704
Gly Asn Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn Ser Ala Leu
        515                 520                 525
ctt ctt tca atc ttg aaa atg ata gat aag tat gat ctt tat ggt caa     1752
Leu Leu Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp Leu Tyr Gly Gln
    530                 535                 540
gta gct tat cct aaa cac cac aag cag tca gat gtt cct gat atc tac     1800
Val Ala Tyr Pro Lys His His Lys Gln Ser Asp Val Pro Asp Ile Tyr
545                 550                 555                 560
cgt ctt gct gca aag act aag ggt gtt ttt att aat cca gct ttt att     1848
Arg Leu Ala Ala Lys Thr Lys Gly Val Phe Ile Asn Pro Ala Phe Ile
                565                 570                 575
gag cct ttt gga ctg act ttg att gag gca gca gct tat ggt ctc cca     1896
Glu Pro Phe Gly Leu Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro
            580                 585                 590
atg gta gcc aca aaa aat gga gga cct gtt gat ata cat agg gtt ctt     1944
Met Val Ala Thr Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu
        595                 600                 605
gac aat ggt ctc tta gtg gat ccc cat gat cag cag gca att gct gat     1992
Asp Asn Gly Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp
    610                 615                 620
gct ctt ttg aag ttg gtt gct gat aag caa ctg tgg gct aaa tgc agg     2040
Ala Leu Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg
625                 630                 635                 640
gca aat gga tta aaa aat atc cac ctt ttc tca tgg ccc gag cac tgt     2088
Ala Asn Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys
                645                 650                 655
aaa act tat cta tcc cgg ata gct agc tgc aaa cca agg caa cca cgc     2136
Lys Thr Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln Pro Arg
            660                 665                 670
tgg ctg aga tcc att gat gat gat gat gaa aat tca gaa aca gat tca     2184
Trp Leu Arg Ser Ile Asp Asp Asp Asp Glu Asn Ser Glu Thr Asp Ser
        675                 680                 685
cct agt gat tcc ttg aga gat att cat gat ata tct ctg aat ttg aga     2232
Pro Ser Asp Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu Arg
    690                 695                 700
ttt tca tta gat ggg gaa aag aat gac aat aaa gaa aat gct gat aat     2280
Phe Ser Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn
705                 710                 715                 720
aca tta gac ccc gaa gtt cga agg agc aag tta gag aat gct gtt ttg     2328
Thr Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn Ala Val Leu
                725                 730                 735
tcc tta tct aag ggt gca ctg aag agc aca tca aaa tct tgg tcg tca     2376
Ser Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys Ser Trp Ser Ser
            740                 745                 750
gac aag gca gac caa aac cct ggt gct ggt aaa ttc cca gcg att agg     2424
Asp Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys Phe Pro Ala Ile Arg
        755                 760                 765
agg agg cga cat att ttt gtt att gca gtg gat tgt gat gct agc tca     2472
Arg Arg Arg His Ile Phe Val Ile Ala Val Asp Cys Asp Ala Ser Ser
    770                 775                 780
gga ctc tct gga agt gtg aaa aag ata ttt gag gct gta gag aag gaa     2520
Gly Leu Ser Gly Ser Val Lys Lys Ile Phe Glu Ala Val Glu Lys Glu
785                 790                 795                 800
agg gca gag ggt tcc att gga ttt atc ctg gct aca tct ttc aat ata     2568
Arg Ala Glu Gly Ser Ile Gly Phe Ile Leu Ala Thr Ser Phe Asn Ile
                805                 810                 815
tca gaa gta cag tct ttc ctg ctt tca gag ggc atg aat cct act gat     2616
Ser Glu Val Gln Ser Phe Leu Leu Ser Glu Gly Met Asn Pro Thr Asp
            820                 825                 830
ttt gat gct tac ata tgc aat agt ggt ggt gat ctt tat tat tcg tcc     2664
Phe Asp Ala Tyr Ile Cys Asn Ser Gly Gly Asp Leu Tyr Tyr Ser Ser
        835                 840                 845
ttc cat tct gag caa aat cct ttt gta gtt gac ttg tac tat cac tca     2712
Phe His Ser Glu Gln Asn Pro Phe Val Val Asp Leu Tyr Tyr His Ser
    850                 855                 860
cat att gag tat cgt tgg ggg ggc gaa gga ttg aga aag act ttg gtg     2760
His Ile Glu Tyr Arg Trp Gly Gly Glu Gly Leu Arg Lys Thr Leu Val
865                 870                 875                 880
cgt tgg gcc gcc tct atc att gat aag aat ggt gaa aat gga gat cac     2808
Arg Trp Ala Ala Ser Ile Ile Asp Lys Asn Gly Glu Asn Gly Asp His
                885                 890                 895
att gtt gtt gag gat gaa gac aat tca gct gac tac tgc tat act ttc     2856
Ile Val Val Glu Asp Glu Asp Asn Ser Ala Asp Tyr Cys Tyr Thr Phe
            900                 905                 910
aaa gtc tgc aag cct ggg acg gtt cct cca tct aaa gag ctt aga aaa     2904
Lys Val Cys Lys Pro Gly Thr Val Pro Pro Ser Lys Glu Leu Arg Lys
        915                 920                 925
gta atg cga att cag gca ctt cgt tgt cac gct gtt tat tgt caa aat     2952
Val Met Arg Ile Gln Ala Leu Arg Cys His Ala Val Tyr Cys Gln Asn
    930                 935                 940
ggg agt agg att aat gtg atc cct gta ctg gca tct cgg tcc caa gca     3000
Gly Ser Arg Ile Asn Val Ile Pro Val Leu Ala Ser Arg Ser Gln Ala
945                 950                 955                 960
ctc agg tac tta tat ctg cga tgg gga atg gac ttg tcg aag ttg gtg     3048
Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu Val
                965                 970                 975
gtt ttc gtc gga gaa agt ggt gat acc gat tat gaa gga tta atc ggt     3096
Val Phe Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu Ile Gly
            980                 985                 990
ggt cta cgc aag gct gtc ata atg aaa ggc ctc tgc act aat gca agc     3144
Gly Leu Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr Asn Ala Ser
        995                1000                1005
agc tta att cac ggt aat agg aat tac ccg cta tct gat gtt tta cca     3192
Ser Leu Ile His Gly Asn Arg Asn Tyr Pro Leu Ser Asp Val Leu Pro
   1010                1015                1020
ttc gac agc cct aat gtc atc caa gcg gac gag gaa tgt agc agc acc     3240
Phe Asp Ser Pro Asn Val Ile Gln Ala Asp Glu Glu Cys Ser Ser Thr
1025               1030                1035                1040
gaa atc cgt tgc tta ctg gag aaa cta gcg gta ctc aaa gga             3282
Glu Ile Arg Cys Leu Leu Glu Lys Leu Ala Val Leu Lys Gly
               1045                1050
taataccctt ccccctttga ttgtcaaaaa cctatatgag ctataagact atgccatgaa   3342
aagaatggcc atccatttgg cttgtctttt gaagctgtta atacttttca acagactaca   3402
aaatgagatg agtcctttga tcctctttaa aggacataaa agctttatgc aagaaccagt   3462
gctgtaaagt tatagaattt cttttgctat atatgacatt cgacagaacc agttccggtt   3522
catcgagaaa aagaaataaa tttcaactta taaacatgcc tgatcatgta aattatcata   3582
tacatccatc ggaaggcatt atcgatgggt tatcagattt ttt                     3625
 
           
             4 
             1054 
             PRT 
             Solanum tuberosum 
           
            4
Met Ala Gly Asn Asp Trp Ile Asn Ser Tyr Leu Glu Ala Ile Leu Asp
  1               5                  10                  15
Val Gly Pro Gly Leu Asp Asp Lys Lys Ser Ser Leu Leu Leu Arg Glu
             20                  25                  30
Arg Gly Arg Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu Val Ile Thr
         35                  40                  45
Gly Phe Asp Glu Thr Asp Leu His Arg Ser Trp Ile Arg Ala Gln Ala
     50                  55                  60
Thr Arg Ser Pro Gln Arg Arg Asn Thr Arg Leu Glu Asn Met Cys Trp
 65                  70                  75                  80
Arg Ile Trp Asn Leu Ala Arg Gln Lys Lys Gln Leu Glu Gly Glu Gln
                 85                  90                  95
Ala Gln Trp Met Ala Lys Arg Arg Gln Glu Arg Glu Arg Gly Arg Arg
            100                 105                 110
Glu Ala Val Ala Asp Met Ser Glu Asp Leu Ser Glu Gly Glu Lys Gly
        115                 120                 125
Asp Ile Val Ala Asp Met Ser Ser His Gly Glu Ser Thr Arg Gly Arg
    130                 135                 140
Leu Pro Arg Ile Ser Ser Val Glu Thr Met Glu Ala Trp Val Ser Gln
145                 150                 155                 160
Gln Arg Gly Lys Lys Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu
                165                 170                 175
Ile Arg Gly Glu Asn Met Glu Leu Gly Arg Asp Ser Asp Thr Gly Gly
            180                 185                 190
Gln Val Lys Tyr Val Val Glu Leu Ala Arg Ala Leu Gly Ser Met Pro
        195                 200                 205
Gly Val Tyr Arg Val Asp Leu Leu Thr Arg Gln Val Ser Ser Pro Glu
    210                 215                 220
Val Asp Trp Ser Tyr Gly Glu Pro Thr Glu Met Leu Thr Pro Ile Ser
225                 230                 235                 240
Thr Asp Gly Leu Met Thr Glu Met Gly Glu Ser Ser Gly Ala Tyr Ile
                245                 250                 255
Ile Arg Ile Pro Phe Gly Pro Arg Glu Lys Tyr Ile Pro Lys Glu Gln
            260                 265                 270
Leu Trp Pro Tyr Ile Pro Glu Phe Val Asp Gly Ala Leu Asn His Ile
        275                 280                 285
Ile Gln Met Ser Lys Val Leu Gly Glu Gln Ile Gly Ser Gly Tyr Pro
    290                 295                 300
Val Trp Pro Val Ala Ile His Gly His Tyr Ala Asp Ala Gly Asp Ser
305                 310                 315                 320
Ala Ala Leu Leu Ser Gly Ala Leu Asn Val Pro Met Leu Phe Thr Gly
                325                 330                 335
His Ser Leu Gly Arg Asp Lys Leu Glu Gln Leu Leu Ala Gln Gly Arg
            340                 345                 350
Lys Ser Lys Asp Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile
        355                 360                 365
Glu Ala Glu Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser
    370                 375                 380
Thr Arg Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp
385                 390                 395                 400
Pro Ile Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser
                405                 410                 415
Cys Tyr Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met
            420                 425                 430
Glu Phe His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr
        435                 440                 445
Glu Gly Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu
    450                 455                 460
Ile Met Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu
465                 470                 475                 480
Ala Arg Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala Phe
                485                 490                 495
Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn Leu Thr Leu Ile Met
            500                 505                 510
Gly Asn Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn Ser Ala Leu
        515                 520                 525
Leu Leu Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp Leu Tyr Gly Gln
    530                 535                 540
Val Ala Tyr Pro Lys His His Lys Gln Ser Asp Val Pro Asp Ile Tyr
545                 550                 555                 560
Arg Leu Ala Ala Lys Thr Lys Gly Val Phe Ile Asn Pro Ala Phe Ile
                565                 570                 575
Glu Pro Phe Gly Leu Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro
            580                 585                 590
Met Val Ala Thr Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu
        595                 600                 605
Asp Asn Gly Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp
    610                 615                 620
Ala Leu Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg
625                 630                 635                 640
Ala Asn Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys
                645                 650                 655
Lys Thr Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln Pro Arg
            660                 665                 670
Trp Leu Arg Ser Ile Asp Asp Asp Asp Glu Asn Ser Glu Thr Asp Ser
        675                 680                 685
Pro Ser Asp Ser Leu Arg Asp Ile His Asp Ile Ser Leu Asn Leu Arg
    690                 695                 700
Phe Ser Leu Asp Gly Glu Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn
705                 710                 715                 720
Thr Leu Asp Pro Glu Val Arg Arg Ser Lys Leu Glu Asn Ala Val Leu
                725                 730                 735
Ser Leu Ser Lys Gly Ala Leu Lys Ser Thr Ser Lys Ser Trp Ser Ser
            740                 745                 750
Asp Lys Ala Asp Gln Asn Pro Gly Ala Gly Lys Phe Pro Ala Ile Arg
        755                 760                 765
Arg Arg Arg His Ile Phe Val Ile Ala Val Asp Cys Asp Ala Ser Ser
    770                 775                 780
Gly Leu Ser Gly Ser Val Lys Lys Ile Phe Glu Ala Val Glu Lys Glu
785                 790                 795                 800
Arg Ala Glu Gly Ser Ile Gly Phe Ile Leu Ala Thr Ser Phe Asn Ile
                805                 810                 815
Ser Glu Val Gln Ser Phe Leu Leu Ser Glu Gly Met Asn Pro Thr Asp
            820                 825                 830
Phe Asp Ala Tyr Ile Cys Asn Ser Gly Gly Asp Leu Tyr Tyr Ser Ser
        835                 840                 845
Phe His Ser Glu Gln Asn Pro Phe Val Val Asp Leu Tyr Tyr His Ser
    850                 855                 860
His Ile Glu Tyr Arg Trp Gly Gly Glu Gly Leu Arg Lys Thr Leu Val
865                 870                 875                 880
Arg Trp Ala Ala Ser Ile Ile Asp Lys Asn Gly Glu Asn Gly Asp His
                885                 890                 895
Ile Val Val Glu Asp Glu Asp Asn Ser Ala Asp Tyr Cys Tyr Thr Phe
            900                 905                 910
Lys Val Cys Lys Pro Gly Thr Val Pro Pro Ser Lys Glu Leu Arg Lys
        915                 920                 925
Val Met Arg Ile Gln Ala Leu Arg Cys His Ala Val Tyr Cys Gln Asn
    930                 935                 940
Gly Ser Arg Ile Asn Val Ile Pro Val Leu Ala Ser Arg Ser Gln Ala
945                 950                 955                 960
Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu Val
                965                 970                 975
Val Phe Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu Ile Gly
            980                 985                 990
Gly Leu Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr Asn Ala Ser
        995                1000                1005
Ser Leu Ile His Gly Asn Arg Asn Tyr Pro Leu Ser Asp Val Leu Pro
   1010                1015                1020
Phe Asp Ser Pro Asn Val Ile Gln Ala Asp Glu Glu Cys Ser Ser Thr
1025               1030                1035                1040
Glu Ile Arg Cys Leu Leu Glu Lys Leu Ala Val Leu Lys Gly
               1045                1050
 
           
             5 
             2930 
             DNA 
             Solanum tuberosum 
             
               CDS 
               (118)..(2841) 
             
           
            5
atttttttct ctaaattctc tctcactgtc cttatcattt caccacctcc ataaatctag     60
aaacatcttt tctattccgt taatctctct agcacacggc ggagtgcggc ggaggag       117
atg gcg gga aac gac tgg att aac agt tac tta gag gcg ata ctg gat      165
Met Ala Gly Asn Asp Trp Ile Asn Ser Tyr Leu Glu Ala Ile Leu Asp
  1               5                  10                  15
gta gga cca ggg cta gat gat aag aaa tca tcg ttg ttg ttg aga gaa      213
Val Gly Pro Gly Leu Asp Asp Lys Lys Ser Ser Leu Leu Leu Arg Glu
             20                  25                  30
aga ggg agg ttt agt ccg acg agg tac ttt gtt gag gaa gtt att act      261
Arg Gly Arg Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu Val Ile Thr
         35                  40                  45
gga ttc gat gag act gat ttg cat cgc tcg tgg atc cga gca caa gct      309
Gly Phe Asp Glu Thr Asp Leu His Arg Ser Trp Ile Arg Ala Gln Ala
     50                  55                  60
act cgg agt ccg cag gag agg aat act agg ctc gag aat atg tgc tgg      357
Thr Arg Ser Pro Gln Glu Arg Asn Thr Arg Leu Glu Asn Met Cys Trp
 65                  70                  75                  80
agg att tgg aat ttg gct cgc cag aaa aag cag ctt gag gga gag caa      405
Arg Ile Trp Asn Leu Ala Arg Gln Lys Lys Gln Leu Glu Gly Glu Gln
                 85                  90                  95
gct cag tgg atg gca aaa cgc cgt caa gaa cgt gag aga ggt cgc aga      453
Ala Gln Trp Met Ala Lys Arg Arg Gln Glu Arg Glu Arg Gly Arg Arg
            100                 105                 110
gaa gca gtt gct gat atg tca gag gat cta tct gag gga gag aaa gga      501
Glu Ala Val Ala Asp Met Ser Glu Asp Leu Ser Glu Gly Glu Lys Gly
        115                 120                 125
gat ata gtc gct gac atg tca tct cat ggt gaa agt acc aga ggc cga      549
Asp Ile Val Ala Asp Met Ser Ser His Gly Glu Ser Thr Arg Gly Arg
    130                 135                 140
ttg cct aga atc agt tct gtt gag aca atg gaa gca tgg gtc agt cag      597
Leu Pro Arg Ile Ser Ser Val Glu Thr Met Glu Ala Trp Val Ser Gln
145                 150                 155                 160
cag aga gga aag aag ctt tat atc gtg ctt ata agt tta cat ggt tta      645
Gln Arg Gly Lys Lys Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu
                165                 170                 175
att cgg ggt gag aat atg gag ctt gga cgg gat tct gat act ggt ggt      693
Ile Arg Gly Glu Asn Met Glu Leu Gly Arg Asp Ser Asp Thr Gly Gly
            180                 185                 190
cag gtg aag tat gta gtt gga gca act gtt gca caa ggt cgt ttg tca      741
Gln Val Lys Tyr Val Val Gly Ala Thr Val Ala Gln Gly Arg Leu Ser
        195                 200                 205
aag gat gaa ata aac tca acc tac aag ata atg cgg aga ata gag gct      789
Lys Asp Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala
    210                 215                 220
gaa gaa tta act ctt gat gct tcc gaa att gtc atc act agt aca aga      837
Glu Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser Thr Arg
225                 230                 235                 240
cag gag att gac gag caa tgg cgt ttg tat gat ggg ttt gat cca ata      885
Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp Pro Ile
                245                 250                 255
tta gag cgt aag tta cgt gca agg atc aag cgc aat gtc agc tgt tat      933
Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser Cys Tyr
            260                 265                 270
ggc agg ttt atg cct cgt atg gct gta att cct cct ggg atg gag ttc      981
Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met Glu Phe
        275                 280                 285
cac cat att gtg cca cat gaa ggt gac atg gat ggt gaa aca gaa gga     1029
His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr Glu Gly
    290                 295                 300
agt gaa gat gga aag acc ccg gat cca cct att tgg gca gag att atg     1077
Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu Ile Met
305                 310                 315                 320
cgc ttc ttt tct aat cca agg aag cct atg ata ctc gca ctt gct agg     1125
Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu Ala Arg
                325                 330                 335
cct gat ccc aag aag aac ctc act act tta gtg aaa gca ttt ggt gaa     1173
Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala Phe Gly Glu
            340                 345                 350
tgt cgt cca ttg aga gac ctt gct aat ctt act ttg ata atg ggt aat     1221
Cys Arg Pro Leu Arg Asp Leu Ala Asn Leu Thr Leu Ile Met Gly Asn
        355                 360                 365
cga gat aat atc gac gaa atg tct agc acc aat tct gca ctt ctt ctt     1269
Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn Ser Ala Leu Leu Leu
    370                 375                 380
tca atc ttg aag atg ata gat aag tat gat ctt tat ggt cta gta gct     1317
Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp Leu Tyr Gly Leu Val Ala
385                 390                 395                 400
tat cct aaa cac cac aag cag tca gat gtt cct gat atc tac cgt ctt     1365
Tyr Pro Lys His His Lys Gln Ser Asp Val Pro Asp Ile Tyr Arg Leu
                405                 410                 415
gct gca aag act aag ggt gtt ttt att aat cca gct ttt att gag cct     1413
Ala Ala Lys Thr Lys Gly Val Phe Ile Asn Pro Ala Phe Ile Glu Pro
            420                 425                 430
ttt gga ctg act ttg att gag gca gca gct tat ggt ctc cca atg gta     1461
Phe Gly Leu Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro Met Val
        435                 440                 445
gcc aca aaa aat gga gga cct gtt gat ata cat agg gtt ctt gac aat     1509
Ala Thr Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu Asp Asn
    450                 455                 460
ggt ctc tta gtg gat ccc cat gat cag cag gca att gct gat gct ctt     1557
Gly Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp Ala Leu
465                 470                 475                 480
ttg aag ttg gtt gct gat aag caa ctg tgg gct aaa tgc agg gca aat     1605
Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg Ala Asn
                485                 490                 495
gga tta aaa aat atc cac ctt ttc tca tgg ccc gag cac tgt aaa act     1653
Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys Lys Thr
            500                 505                 510
tat cta tcc cgg ata gct agc tgc aaa ccg agg caa cat tcc ttg aga     1701
Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln His Ser Leu Arg
        515                 520                 525
gat att cat gat ata tct ctg aat ttg aga ttt tca tta gat ggg gaa     1749
Asp Ile His Asp Ile Ser Leu Asn Leu Arg Phe Ser Leu Asp Gly Glu
    530                 535                 540
aag aat gac aat aaa gaa aat gct gat aat aca tta gac ccc gaa gtt     1797
Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn Thr Leu Asp Pro Glu Val
545                 550                 555                 560
cga agg agc aag tta gag aat gct gtt ttg tcc tta tct aag ggt gca     1845
Arg Arg Ser Lys Leu Glu Asn Ala Val Leu Ser Leu Ser Lys Gly Ala
                565                 570                 575
ctg aag agc aca tca aaa tct tgg tcg tca gac aag gca gac caa aat     1893
Leu Lys Ser Thr Ser Lys Ser Trp Ser Ser Asp Lys Ala Asp Gln Asn
            580                 585                 590
cct ggt gct ggt aaa ttc cca gcg att agg agg agg cga cat att ttt     1941
Pro Gly Ala Gly Lys Phe Pro Ala Ile Arg Arg Arg Arg His Ile Phe
        595                 600                 605
gtt att gca gtg gat tgt gat gct agc tca gga ctc tct gga agt atg     1989
Val Ile Ala Val Asp Cys Asp Ala Ser Ser Gly Leu Ser Gly Ser Met
    610                 615                 620
aaa aag ata ttt gag gct gta gag aag gaa agg gca gag ggt tcc att     2037
Lys Lys Ile Phe Glu Ala Val Glu Lys Glu Arg Ala Glu Gly Ser Ile
625                 630                 635                 640
gga ttt atc ctt gct aca tct ttc aat ata tca gaa gta cag tct ttc     2085
Gly Phe Ile Leu Ala Thr Ser Phe Asn Ile Ser Glu Val Gln Ser Phe
                645                 650                 655
ctg ctt tca gag ggc atg aat cct act gag caa aat cct ttt gta gtt     2133
Leu Leu Ser Glu Gly Met Asn Pro Thr Glu Gln Asn Pro Phe Val Val
            660                 665                 670
gac ttg tac tat cac tca cat att gag tat cgt tgg ggg ggc gaa ggg     2181
Asp Leu Tyr Tyr His Ser His Ile Glu Tyr Arg Trp Gly Gly Glu Gly
        675                 680                 685
ttg aga aag act ttg gtg cgt tgg gcc gcc tct atc att gat aag aat     2229
Leu Arg Lys Thr Leu Val Arg Trp Ala Ala Ser Ile Ile Asp Lys Asn
    690                 695                 700
ggt gaa aat gga gat cac att gtt gtt gag gat gaa gac aat tca gct     2277
Gly Glu Asn Gly Asp His Ile Val Val Glu Asp Glu Asp Asn Ser Ala
705                 710                 715                 720
gac tac tgc tat aca ttc aaa gtt tgc aag cct ggg acg gtt cct cca     2325
Asp Tyr Cys Tyr Thr Phe Lys Val Cys Lys Pro Gly Thr Val Pro Pro
                725                 730                 735
tct aaa gaa ctt aga aaa gta atg cga att cag gca ctt cgt tgt cac     2373
Ser Lys Glu Leu Arg Lys Val Met Arg Ile Gln Ala Leu Arg Cys His
            740                 745                 750
gct gtt tat tgt caa aat ggg agt agg att aat gtg atc cct gta ctg     2421
Ala Val Tyr Cys Gln Asn Gly Ser Arg Ile Asn Val Ile Pro Val Leu
        755                 760                 765
gca tct cgg tcc caa gca ctc agg tac tta tat ctg cga tgg gga atg     2469
Ala Ser Arg Ser Gln Ala Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met
    770                 775                 780
gtc cct gta ctg gca tct cgg tcc caa gca ctc agg tac tta tat ctg     2517
Val Pro Val Leu Ala Ser Arg Ser Gln Ala Leu Arg Tyr Leu Tyr Leu
785                 790                 795                 800
cga tgg gga atg gtc cct gta ctg gca tct cgg tcc caa gca ctc agg     2565
Arg Trp Gly Met Val Pro Val Leu Ala Ser Arg Ser Gln Ala Leu Arg
                805                 810                 815
tac tta tat ctg cga tgg gga atg gac ttg tcg aag ttg gtg gtt ttc     2613
Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu Val Val Phe
            820                 825                 830
gtc gga gaa agt ggt gat acc gat tat gaa gga ttg atc ggt ggt cta     2661
Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu Ile Gly Gly Leu
        835                 840                 845
cgc aag gct gtc ata atg aaa gga ctc tgc act aat gca agc agc tta     2709
Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr Asn Ala Ser Ser Leu
    850                 855                 860
att cac ggt aat agg aat tac ccg cta tct gat gtt tta cca ttc gag     2757
Ile His Gly Asn Arg Asn Tyr Pro Leu Ser Asp Val Leu Pro Phe Glu
865                 870                 875                 880
agc cct aat gtc atc caa gcg gat gag gaa tgt agc agc acc gga atc     2805
Ser Pro Asn Val Ile Gln Ala Asp Glu Glu Cys Ser Ser Thr Gly Ile
                885                 890                 895
cgt tcc tta ctg gag aaa cta gcg gta ctc aaa gga taataccctt          2851
Arg Ser Leu Leu Glu Lys Leu Ala Val Leu Lys Gly
            900                 905
ccccctttga ttgtcaaaaa cctatatgag ctaagattat gccatgaaaa gaatggccat   2911
ccatttggct tgtcttttg                                                2930
 
           
             6 
             908 
             PRT 
             Solanum tuberosum 
           
            6
Met Ala Gly Asn Asp Trp Ile Asn Ser Tyr Leu Glu Ala Ile Leu Asp
  1               5                  10                  15
Val Gly Pro Gly Leu Asp Asp Lys Lys Ser Ser Leu Leu Leu Arg Glu
             20                  25                  30
Arg Gly Arg Phe Ser Pro Thr Arg Tyr Phe Val Glu Glu Val Ile Thr
         35                  40                  45
Gly Phe Asp Glu Thr Asp Leu His Arg Ser Trp Ile Arg Ala Gln Ala
     50                  55                  60
Thr Arg Ser Pro Gln Glu Arg Asn Thr Arg Leu Glu Asn Met Cys Trp
 65                  70                  75                  80
Arg Ile Trp Asn Leu Ala Arg Gln Lys Lys Gln Leu Glu Gly Glu Gln
                 85                  90                  95
Ala Gln Trp Met Ala Lys Arg Arg Gln Glu Arg Glu Arg Gly Arg Arg
            100                 105                 110
Glu Ala Val Ala Asp Met Ser Glu Asp Leu Ser Glu Gly Glu Lys Gly
        115                 120                 125
Asp Ile Val Ala Asp Met Ser Ser His Gly Glu Ser Thr Arg Gly Arg
    130                 135                 140
Leu Pro Arg Ile Ser Ser Val Glu Thr Met Glu Ala Trp Val Ser Gln
145                 150                 155                 160
Gln Arg Gly Lys Lys Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu
                165                 170                 175
Ile Arg Gly Glu Asn Met Glu Leu Gly Arg Asp Ser Asp Thr Gly Gly
            180                 185                 190
Gln Val Lys Tyr Val Val Gly Ala Thr Val Ala Gln Gly Arg Leu Ser
        195                 200                 205
Lys Asp Glu Ile Asn Ser Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala
    210                 215                 220
Glu Glu Leu Thr Leu Asp Ala Ser Glu Ile Val Ile Thr Ser Thr Arg
225                 230                 235                 240
Gln Glu Ile Asp Glu Gln Trp Arg Leu Tyr Asp Gly Phe Asp Pro Ile
                245                 250                 255
Leu Glu Arg Lys Leu Arg Ala Arg Ile Lys Arg Asn Val Ser Cys Tyr
            260                 265                 270
Gly Arg Phe Met Pro Arg Met Ala Val Ile Pro Pro Gly Met Glu Phe
        275                 280                 285
His His Ile Val Pro His Glu Gly Asp Met Asp Gly Glu Thr Glu Gly
    290                 295                 300
Ser Glu Asp Gly Lys Thr Pro Asp Pro Pro Ile Trp Ala Glu Ile Met
305                 310                 315                 320
Arg Phe Phe Ser Asn Pro Arg Lys Pro Met Ile Leu Ala Leu Ala Arg
                325                 330                 335
Pro Asp Pro Lys Lys Asn Leu Thr Thr Leu Val Lys Ala Phe Gly Glu
            340                 345                 350
Cys Arg Pro Leu Arg Asp Leu Ala Asn Leu Thr Leu Ile Met Gly Asn
        355                 360                 365
Arg Asp Asn Ile Asp Glu Met Ser Ser Thr Asn Ser Ala Leu Leu Leu
    370                 375                 380
Ser Ile Leu Lys Met Ile Asp Lys Tyr Asp Leu Tyr Gly Leu Val Ala
385                 390                 395                 400
Tyr Pro Lys His His Lys Gln Ser Asp Val Pro Asp Ile Tyr Arg Leu
                405                 410                 415
Ala Ala Lys Thr Lys Gly Val Phe Ile Asn Pro Ala Phe Ile Glu Pro
            420                 425                 430
Phe Gly Leu Thr Leu Ile Glu Ala Ala Ala Tyr Gly Leu Pro Met Val
        435                 440                 445
Ala Thr Lys Asn Gly Gly Pro Val Asp Ile His Arg Val Leu Asp Asn
    450                 455                 460
Gly Leu Leu Val Asp Pro His Asp Gln Gln Ala Ile Ala Asp Ala Leu
465                 470                 475                 480
Leu Lys Leu Val Ala Asp Lys Gln Leu Trp Ala Lys Cys Arg Ala Asn
                485                 490                 495
Gly Leu Lys Asn Ile His Leu Phe Ser Trp Pro Glu His Cys Lys Thr
            500                 505                 510
Tyr Leu Ser Arg Ile Ala Ser Cys Lys Pro Arg Gln His Ser Leu Arg
        515                 520                 525
Asp Ile His Asp Ile Ser Leu Asn Leu Arg Phe Ser Leu Asp Gly Glu
    530                 535                 540
Lys Asn Asp Asn Lys Glu Asn Ala Asp Asn Thr Leu Asp Pro Glu Val
545                 550                 555                 560
Arg Arg Ser Lys Leu Glu Asn Ala Val Leu Ser Leu Ser Lys Gly Ala
                565                 570                 575
Leu Lys Ser Thr Ser Lys Ser Trp Ser Ser Asp Lys Ala Asp Gln Asn
            580                 585                 590
Pro Gly Ala Gly Lys Phe Pro Ala Ile Arg Arg Arg Arg His Ile Phe
        595                 600                 605
Val Ile Ala Val Asp Cys Asp Ala Ser Ser Gly Leu Ser Gly Ser Met
    610                 615                 620
Lys Lys Ile Phe Glu Ala Val Glu Lys Glu Arg Ala Glu Gly Ser Ile
625                 630                 635                 640
Gly Phe Ile Leu Ala Thr Ser Phe Asn Ile Ser Glu Val Gln Ser Phe
                645                 650                 655
Leu Leu Ser Glu Gly Met Asn Pro Thr Glu Gln Asn Pro Phe Val Val
            660                 665                 670
Asp Leu Tyr Tyr His Ser His Ile Glu Tyr Arg Trp Gly Gly Glu Gly
        675                 680                 685
Leu Arg Lys Thr Leu Val Arg Trp Ala Ala Ser Ile Ile Asp Lys Asn
    690                 695                 700
Gly Glu Asn Gly Asp His Ile Val Val Glu Asp Glu Asp Asn Ser Ala
705                 710                 715                 720
Asp Tyr Cys Tyr Thr Phe Lys Val Cys Lys Pro Gly Thr Val Pro Pro
                725                 730                 735
Ser Lys Glu Leu Arg Lys Val Met Arg Ile Gln Ala Leu Arg Cys His
            740                 745                 750
Ala Val Tyr Cys Gln Asn Gly Ser Arg Ile Asn Val Ile Pro Val Leu
        755                 760                 765
Ala Ser Arg Ser Gln Ala Leu Arg Tyr Leu Tyr Leu Arg Trp Gly Met
    770                 775                 780
Val Pro Val Leu Ala Ser Arg Ser Gln Ala Leu Arg Tyr Leu Tyr Leu
785                 790                 795                 800
Arg Trp Gly Met Val Pro Val Leu Ala Ser Arg Ser Gln Ala Leu Arg
                805                 810                 815
Tyr Leu Tyr Leu Arg Trp Gly Met Asp Leu Ser Lys Leu Val Val Phe
            820                 825                 830
Val Gly Glu Ser Gly Asp Thr Asp Tyr Glu Gly Leu Ile Gly Gly Leu
        835                 840                 845
Arg Lys Ala Val Ile Met Lys Gly Leu Cys Thr Asn Ala Ser Ser Leu
    850                 855                 860
Ile His Gly Asn Arg Asn Tyr Pro Leu Ser Asp Val Leu Pro Phe Glu
865                 870                 875                 880
Ser Pro Asn Val Ile Gln Ala Asp Glu Glu Cys Ser Ser Thr Gly Ile
                885                 890                 895
Arg Ser Leu Leu Glu Lys Leu Ala Val Leu Lys Gly
            900                 905