PATENT DOCUMENT

Abstract:
Adenoviral serotypes differ in their natural tropism. The various serotypes of adenovirus have been found to differ in at least their capsid proteins (e.g., penton-base and hexon proteins), proteins responsible for cell binding (e.g, fiber proteins), and proteins involved in adenovirus replication. This difference in tropism and capsid proteins among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins. The present invention bypasses such requirement for capsid protein modification as it presents a recombinant, replication-defective adenovirus of serotype 34, a rare adenoviral serotype, and methods for generating the alternative, recombinant adenovirus. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided.

Full Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
       [0001]    This application claims the benefit of U.S. provisional application serial No. 60/458,825, filed on Mar. 28, 2003. 
     
    
     
       BACKGROUND OF THE INVENTION  
         [0002]    Adenoviruses are nonenveloped, icosahedral viruses that have been identified in several avian and mammalian hosts; Home et al., 1959  J. Mol. Biol . 1:84-86; Horwitz, 1990 In  Virology , eds. B. N. Fields and D. M. Knipe, pps. 1679-1721. The first human adenoviruses (Ads) were isolated over four decades ago. Since then, over 100 distinct adenoviral serotypes have been isolated which infect various mammalian species, 51 of which are of human origin; Straus, 1984, In  The Adenoviruses , ed. H. Ginsberg, pps. 451-498, New York:Plenus Press; Hierholzer et al., 1988  J. Infect. Dis . 158:804-813; Schnurr and Dondero, 1993,  Intervirology;  36:79-83; Jong et al., 1999  J Clin Microbiol ., 37:3940-5. The human serotypes have been categorized into six subgenera (A-F) based on a number of biological, chemical, immunological and structural criteria which include hemagglutination properties of rat and rhesus monkey erythrocytes, DNA homology, restriction enzyme cleavage patterns, percentage G+C content and oncogenicity; Straus, supra; Horwitz, supra.  
           [0003]    The adenovirus genome is very well characterized. It consists of a linear double-stranded DNA molecule of approximately 36,000 base pairs, and despite the existence of several distinct serotypes, there is some general conservation in the overall organization of the adenoviral genome with specific functions being similarly positioned.  
           [0004]    Adenovirus has been a very attractive target for delivery of exogenous genes. The biology of adenoviruses is very well understood. Adenovirus has not been found to be associated with severe human pathology in immuno-competent individuals. The virus is extremely efficient in introducing its DNA into the host cell and is able to infect a wide variety of cells. Furthermore, the virus can be produced at high virus titers in large quantities. In addition, the virus can be rendered replication defective by deletion of the essential early-region 1 (E1) of the viral genome; Brody et al, 1994  Ann N Y Acad Sci.,  716:90-101.  
           [0005]    Replication-defective adenovirus vectors have been used extensively as gene transfer vectors for vaccine and gene therapy purposes. These vectors are propagated in cell lines that provide E1 gene products in trans. Supplementation of the essential E1 gene products in trans is very effective when the vectors are from the same or a very similar serotype. E1-deleted group C serotypes (Ad1, Ad2, Ad5 and Ad6), for instance, grow well in 293 or PER.C6 cells which contain and express the Ad5 E1 region. However, the Ad5 E1 sequences in 293 or PER.C6 cells do not fully complement the replication of all serotypes other than group C. This is perhaps due to the inability of the Ad5 (group C) E1B 55K gene product to functionally interact with the E4 gene product(s) of the non-group C serotypes. Although the interaction is conserved within members of the same subgroup, it has not been found to be well conserved between subgroups. In order to successfully and efficiently rescue recombinant adenovirus of alternative, non-group C serotypes, a cell line expressing the E1 region of the serotype of interest would have to be generated. Alternatively, available Ad5E1-expressing cell lines could be modified to express Ad5E4 (or Orf6) in addition to Ad5E1. These additional, sometimes tedious and daunting tasks, impeded the production of recombinant, non-group C adenoviral vectors.  
           [0006]    An efficient means for the propagation and rescue of alternative serotypes in an Ad5 E1-expressing cell line (such as PER.C6 or 293) was disclosed in pending U.S. provisional application (Serial No. 60/405,182, filed Aug. 22, 2002). This method involves the incorporation of a critical E4 region into the adenovirus to be propagated. The critical E4 region is native to a virus of the same or highly similar serotype as that of the E1 gene product(s), particularly the E1B 55K region, of the complementing cell line, and comprises, in the least, nucleic acid encoding E4 Orf6.  
           [0007]    Presently, two well-characterized adenovirus serotypes from subgroup C, Ad5 and Ad2, are the most widely used gene delivery vectors. There is a need to develop alternate Ad serotypes as gene transfer vectors since neutralizing antibodies in the general population may limit primary dosing or redosing with the same serotype. The prevalence of neutralizing antibody can vary from serotype to serotype. Neutralizing antibodies to some serotypes such as Ad5 are common, while antibodies to others are relatively rare. Alternate serotypes, furthermore, possess alternate tropisms which may lead to the elicitation of superior immune responses when used for vaccine or gene therapy purposes.  
           [0008]    Adenovirus serotype 34, a subgroup B adenovirus, was originally isolated in 1972 and established as a recognized reference strain in 1975 (J. C. Hierholzer et al., 1975  J. Clin. Microbiol.  1:366-376). Its antigenic relationship to 46 other human adenoviruses determined in reference horse antisera has been discussed; J. C. Hierholzer et al., 1991  Arch. Virol.  121:179-197. Partial sequence information is available for Ad34. There have been several disclosures relating to Ad34 hexon sequences. The complete sequence of Ad34 hexon with some 5′ and 3′ flanking sequence (3358 bp) was deposited in GenBank (Accession No. AB052911) by Mukouyama. A partial sequence of Ad34 hexon (1449 bp) was disclosed in Takeuchi et al., 1999  J. Clin. Microbiol.  37:3392-3394, and GenBank (Accession No. AB018426). A partial sequence of Ad34 hexon (253 bp) was disclosed in Allard et al, 2001  J. Clin. Microbiol.  39: 498-505 , and deposited in GenBank (Accession No. AF161573). Perera and Cardosa deposited two partial sequences of Ad34 hexon (571bp and 301 bp) with GenBank (Accession Nos. AJ272610 and AJ250786). Sequence for the Ad34 fiber gene was deposited by Arun, Mukouyama and Inada with GenBank (Accession No. AB073168). The sequence of the virus associated RNA region (VA RNA1 &amp; 2) for Ad34 (162 bp) was disclosed by Kidd et al., 1995  Virology  207:32-45, and GenBank (Accession No. U10677). Moreover, the sequence of the virus associated RNA region for Ad34 and partial sequence for the pre-terminal protein and 52/55K proteins (354 bp) was disclosed in Ma &amp; Matthews, 1996  J. Virol.  70: 5083-99, and GenBank (Accession No. U52571). Adhikary, Mukouyama and Inada disclosed the sequence of the Ad34 genes for L4 100kDa, L4pVIII, E3 12.3kDa, E3 14.9kDa, E3 gp18.5kDa, E3 20.3kDa, E3 20.5kDa, E3 10.2kDa, E3 15.2kDal, E3 15.2kDa2, and partial fiber sequence (4828 bp) and deposited the sequence with GenBank (Accession No. AB079724). The sequence of the right end of the viral genome (1038 bp) was disclosed in Chen &amp; Horwitz, 1990  Virology  179:567-75, and GenBank (Accession No. M62712).  
           [0009]    The fields of vaccines and gene therapy would greatly benefit from additional knowledge concerning alternative adenoviral serotypes, particularly those serotypes such as Ad34 which are not well represented in the human population. Of particular interest are recombinant adenoviral vectors based on alternative adenoviral serotypes, and means of obtaining such recombinant adenoviral vectors. This need in the art is met with the disclosure of the present application related to recombinant adenoviral vectors based on adenoviral serotype 34.  
         SUMMARY OF THE INVENTION  
         [0010]    The present invention relates to recombinant, replication-deficient adenovirus vectors of serotype 34, a rare adenoviral serotype, and methods for generating the recombinant adenovirus based on the alternative serotype. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided. The invention, thus, encompasses recombinant, replication-defective adenoviral vectors of serotype 34 which comprise one or more transgenes operatively linked to regulatory sequences which promote effective expression of the respective transgene(s). Host administration of such recombinant adenovirus serotype 34 vectors, whether administered alone or in a combined modality and/or prime boost regimen, results in the efficient expression of the incorporated transgene and effectively induces an immune response capable of specifically recognizing the particular antigen administered (e.g., HIV). Furthermore, the recombinant virus should evade pre-existing immunity to adenovirus serotypes which are more commonly encountered in the human population (e.g., Ad5 and Ad2). The disclosed methods, thus, present an enhanced means for inducing an immune response against a particular antigen of interest (e.g., HIV). Accordingly, the resultant immune response should offer a prophylactic advantage to previously uninfected individuals and/or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of infection. 
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0011]    [0011]FIG. 1 illustrates the homologous recombination scheme utilized to recover pAd34ΔE1ΔE4Ad5Orf6.  
         [0012]    [0012]FIG. 2 illustrates the homologous recombination scheme utilized to recover pMRKAd34ΔE1ΔE4Ad5Orf6.  
         [0013]    [0013]FIGS. 3A-1 to  3 A- 9  illustrate a nucleic acid sequence for wild-type adenovirus serotype 34 (SEQ ID NO: 1). The ATCC product number for Ad34 is VR-716.  
         [0014]    [0014]FIG. 4 illustrates the time course of SEAP expression using MRKAd5 and Ad34 vectors in rhesus macaques. Data represent cohort geometric means.  
         [0015]    [0015]FIG. 5 illustrates, in tabular format, T cell responses induced using MRKAd5 and Ad34 vectors expressing HIV-1 gag. Data are expressed in numbers of spot-forming cells per million PBMC (SFC/10{circumflex over ( )}6 PBMC). “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.  
         [0016]    [0016]FIG. 6 illustrates, in tabular format, the levels of CD4+ and CD8+ Gag-specific T cells in Ad34-immunized macaques at week 12. “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.  
         [0017]    [0017]FIG. 7 illustrates the nucleic acid sequence (SEQ ID NO: 3) of the optimized human HIV-1 gag open reading frame.  
         [0018]    [0018]FIG. 8 illustrates the nucleic acid sequence encoding the gag expression cassette (SEQ ID NO: 4). The various regions of the figure are as follows: (1) a first underlined segment of nucleic acid sequence encoding the immediate early gene promoter region from human cytomegalovirus; (2) a first segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; (3) a region in caps which contains the coding sequence of HIV-1 gag; (4) a second segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; and (5) a second underlined segment, this segment containing nucleic acid sequence encoding a bovine growth hormone polyadenylation signal sequence.  
         [0019]    [0019]FIG. 9 illustrates the nucleic acid sequence encoding the SEAP expression cassette (SEQ ID NO: 5). The various regions of the figure are as follows: (1) a first underlined segment of nucleic acid sequence encoding the immediate early gene promoter region from human cytomegalovirus; (2) a first segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; (3) a region in caps which contains the coding sequence of the human placental SEAP gene; (4) a second segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; and (5) a second underlined segment, this segment containing nucleic acid sequence encoding a bovine growth hormone polyadenylation signal sequence.  
         [0020]    [0020]FIGS. 10A-1 to  10 A- 47  illustrate the nucleotide sequence of the pMRKAd5HIV-1 gag vector (SEQ ID NO: 6 [coding] and SEQ ID NO: 7 [non-coding]).  
         [0021]    [0021]FIGS. 11A-1 to  11 A- 10  illustrate a nucleic acid sequence for wild-type adenovirus serotype 35 (SEQ ID NO: 13). The ATCC product number for Ad35 is VR-718.  
         [0022]    [0022]FIG. 12 illustrates, in tabular format, T cell responses induced using a heterologous Ad34 prime/Ad35 boost regimen in macaques. “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.  
         [0023]    [0023]FIG. 13 illustrates, in tabular format, the levels of CD4+ and CD8+ Gag-specific T cells in Ad34 primed/Ad35 boosted macaques at week 28. “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.  
     
    
     DETAILED DESCRIPTION OF THE INVENTION  
       [0024]    Rare adenoviral serotypes possess an inherent advantage over the more commonly exploited adenoviral serotypes (for instance, adenoviral serotypes 2 and 5) since preexisting immunity is unlikely to limit their efficient delivery and expression of exogenous genes to their target site. Different adenoviral serotypes also exhibit distinct tropisms by reason of their varying capsid structure and, thus, present the potential for targeting different tissues and possibly leading to the elicitation of superior immune responses when used for vaccine or gene therapy purposes. These rare adenoviral serotypes when rendered replication-defective, however, can be difficult to propagate and rescue in currently available adenoviral propagation cell lines.  
         [0025]    Applicants have recently managed to successfully rescue and propagate one such rare, replication-defective alternative serotype, adenovirus serotype 34, a subgroup B adenovirus, and herein demonstrate the effective functioning of the adenovirus in the delivery and expression of exogenous transgenes.  
         [0026]    Accordingly, the present invention relates to a recombinant adenoviral vector of serotype 34 suitable for use in gene therapy or vaccination protocols. The nucleic acid sequence for wild-type adenovirus serotype 34 (SEQ ID NO: 1) is illustrated in FIGS. 3A-1 to  3 A- 9 , although any functional homologue or different strain of adenovirus serotype 34 can be utilized in accordance with the methods of the present invention, as one of ordinary skill in the art will appreciate. Ad34 sequence has been noted to differ in a few regions. The following sites are just a sampling of sequence variation that can be found in Ad34: (1) around base pair 10640 of SEQ ID NO: 1, a series of thirteen (“13”) rather than twelve (“12”) “T”s follow the sequence gtgagtccta (SEQ ID NO: 8); (2) around base pair 15372 of SEQ ID NO: 1, a series of fifteen (“15”) or seventeen (“17”) rather than sixteen (“16”) “A”s follow the sequence ccgcactttct (SEQ ID NO: 9); (3) around base pair 17325 of SEQ ID NO: 1, a series of thirteen (“13”) rather than twelve (“12”) “A”s follow the sequence attgacattgg (SEQ ID NO: 10); and (4) around base pair 25717 of SEQ ID NO: 1, the sequence cagtctggagga (SEQ ID NO: 11) following the sequence ggagga (SEQ ID NO: 12) is deleted. Adenovirus serotypes have been distinguished through a number of art-appreciated biological, chemical, immunological and structural criteria which include hemagglutination properties of rat and rhesus monkey erythrocytes, DNA homology, restriction enzyme cleavage patterns, percentage G+C content and oncogenicity; Straus, supra; Horwitz, supra. A given serotype can be identified by a number of methods including restriction mapping of viral DNA; analyzing the mobility of viral DNA; analyzing the mobility of virion polypeptides on SDS-polyacrylamide gels following electrophoresis; comparison of sequence information to known sequence particularly from capsid genes (e.g., hexon) which contain sequences that define a serotype; and comparing a sequence with reference sera for a particular serotype available from the ATCC. Classification of adenovirus serotypes by SDS-PAGE has been discussed in Wadell et al., 1980  Ann. N. Y Acad. Sci.  354:16-42. Classification of adenovirus serotypes by restriction mapping has been discussed in Wadell et al.,  Current Topics in Microbiology and Immunology  110: 191-220. Adenovirus serotype 34, a subgroup B adenovirus, was originally isolated in 1972 and was established as a recognized reference strain in 1975 (J. C. Hierholzer et al., 1975  J. Clin. Microbiol.  1:366-376). Its antigenic relationship to 46 other human adenoviruses determined in reference horse antisera has been discussed in the art; J. C. Hierholzer et al., 1991  Arch. Virol.  121:179-197.  
         [0027]    Adenovirus serotype 34 vectors in accordance with the present invention are at least partially deleted in E1 and devoid (or essentially devoid) of E1 activity, rendering the vector incapable of replication in the intended host. Preferably, the E1 region is completely deleted or inactivated. The adenoviruses may contain additional deletions in E3, and other early regions, albeit in situations where E2 and/or E4 is deleted, E2 and/or E4 complementing cell lines may be required to generate recombinant, replication-defective adenoviral vectors.  
         [0028]    Adenoviral vectors of use in the methods of the present invention can be constructed using well known techniques, such as those reviewed in Hitt et al., 1997 “Human Adenovirus Vectors for Gene Transfer into Mammalian Cells”  Advances in Pharmacology  40:137-206, which is hereby incorporated by reference. Often, a plasmid or shuttle vector containing the heterologous nucleic acid of interest is generated which comprises sequence homologous to the specific adenovirus of interest. The shuttle vector and viral DNA or second plasmid containing the cloned viral DNA are then co-transfected into a host cell where homologous recombination occurs and results in the incorporation of the heterologous nucleic acid into the viral nucleic acid. Preferred shuttle vectors and cloned viral genomes contain adenoviral and plasmid portions. For shuttle vectors used in the construction of replication-defective vectors, the adenoviral portion typically contains non-functional or deleted E1 and E3 regions and the gene expression cassette, flanked by convenient restriction sites. The plasmid portion of the shuttle vector typically contains an antibiotic resistance marker under the transcriptional control of a prokaryotic promoter. Ampicillin resistance genes, neomycin resistance genes and other pharmaceutically acceptable antibiotic resistance markers may be used. To aid in the high level production of the nucleic acid by fermentation in prokaryotic organisms, it is advantageous for the shuttle vector to contain a prokaryotic origin of replication and be of high copy number. A number of commercially available prokaryotic cloning vectors provide these benefits. Non-essential DNA sequences are, preferably removed. It is also preferable that the vectors not be able to replicate in eukaryotic cells. This minimizes the risk of integration of nucleic acid vaccine sequences into the recipients&#39; genome. Tissue-specific promoters or enhancers may be used whenever it is desirable to limit expression of the nucleic acid to a particular tissue type.  
         [0029]    Homologous recombination of the shuttle vector and wild-type adenovirus 34 viral DNA (Ad34 backbone vector) results in the generation of adenoviral pre-plasmids (see, for instance, pAd34ΔE1ΔE4Ad5Orf6, pMRKAd34ΔE1ΔE4Ad5Orf6, pAd34ΔE1gagΔE4Ad5Orf6, and pAd34ΔE1SEAPΔE4Ad5Orf6). Upon linearization, the pre-plasmids are capable of replication in PER.C6® cells or alternative E1-complementing cell lines. Infected cells and media can then be harvested once viral replication is complete.  
         [0030]    A packaging cell will generally be needed in order to produce sufficient amount of adenovirus. The packaging cell should contain elements which are necessary for the production of the specific adenovirus of interest. It is preferable that the packaging cell and the vector not contain overlapping elements which could lead to replication competent virus by recombination. Specific examples of cells which are suitable for the propagation of recombinant Ad34 E1-deleted vectors express the early region 1 (E1) of adenovirus 34 or another group B serotype. Alternatively, propagation cell lines can be used which express adenoviral E1 and E4 regions (particularly, E4 open reading frame 6 (“ORF6”)) which are derived from the same serotype but different subgroup than Ad34 (e.g., Ad5 E1 and E4); see, e.g., Abrahamsen et al., 1997  J. Virol.  8946-8951, and U.S. Pat. No. 5,849,561. Additionally, a cell line could be used that expresses E1B from Ad34 in addition to (1) E1A or (2) E1A and E1B from a serotype of a different subgroup. In copending U.S. provisional application serial No. 60/405,182, filed Aug. 22, 2002, a strategy was disclosed for the efficient propagation and rescue of alternative adenoviral serotypes. The method is based on incorporating, into the genome of the adenovirus vector, an E4 region (or portion thereof including E4 ORF6) of the same or highly similar serotype as that of the E1 gene product(s), particularly E1B, being expressed by the complementing cell line. Examples 1-4 demonstrate the viability of such a method through the incorporation of an Ad5E4 region and its propagation in PER.C6 cells (which cells express Ad5E1). The wildtype adenovirus serotype 5 sequence is known and described in the art; see Chroboczek et al., 1992  J. Virol.  186:280, which is hereby incorporated by reference. Placement of the E4 region or ORF6-containing portion is not critical. The critical step is making sure that either a promoter is supplied or the gene is strategically placed so that it runs off a promoter native to the vector (e.g., such as the E4 promoter). The native E4 region of the vector can be replaced, deleted or left intact. This method is, thus, suitable for use in the propagation and rescue of the adenoviral vectors of the present invention.  
         [0031]    Typically, propagation cells are human cells derived from the retina or kidney, although any cell line capable of expressing the appropriate E1 and/or E4 region(s) can be utilized in the present invention. Embryonal cells such as amniocytes have been shown to be particularly suited for the generation of E1 complementing cell lines. Several cell lines are available. These include but are not limited to the known cell lines PER.C6 (ECACC deposit number 96022940), 911, 293, and E1 A549.  
         [0032]    The present invention encompasses methods for producing a recombinant, replication-defective adenovirus of serotype 34 in an adenoviral E1-complementing cell line, comprising transfecting a recombinant, replication-defective adenoviral vector of serotype 34 in an adenoviral E1-complementing cell and allowing for the production of viral particles. The viral particles so produced form another aspect of the present invention. Host cells comprising the recombinant, replication-defective adenoviral serotype 34 vectors of the present invention form yet another aspect of the present invention; host cells being defined as a population of cells not including a transgenic human being. Recombinant, replication-defective adenovirus harvested in accordance with the methods of the present invention are encompassed herein as well. This harvested material may be purified, formulated and stored prior to host administration.  
         [0033]    Adenoviral vectors in accordance with the present invention are very well suited to effectuate expression of desired proteins, especially in situations where an individual&#39;s immune response effectively prevents administration or readministration via the more commonly employed adenoviral serotypes. Accordingly, specific embodiments of the present invention are recombinant, replication-defective adenoviral vectors of serotype 34 which comprise a heterologous nucleic acid of interest. The nucleic acid of interest can be a gene, or a functional part of a gene. The nucleic acid can be DNA and/or RNA, can be double or single stranded, and can exist in the form of an expression cassette. The nucleic acid can be inserted in an E1 parallel (transcribed 5′ to 3′) or anti-parallel (transcribed in a 3′ to 5′ direction relative to the vector backbone) orientation. The nucleic acid can be codon-optimized for expression in the desired host (e.g., a mammalian host). The heterologous nucleic acid can be in the form of an expression cassette. A gene expression cassette will typically contain (a) nucleic acid encoding a protein or antigen of interest; (b) a heterologous promoter operatively linked to the nucleic acid encoding the protein; and (c) a transcription termination signal.  
         [0034]    In specific embodiments, the heterologous promoter is recognized by an eukaryotic RNA polymerase. One example of a promoter suitable for use in the present invention is the immediate early human cytomegalovirus promoter (Chapman et al., 1991  Nucl. Acids Res.  19:3979-3986). Further examples of promoters that can be used in the present invention are the strong immunoglobulin promoter, the EF1 alpha promoter, the murine CMV promoter, the Rous Sarcoma Virus promoter, the SV40 early/late promoters and the beta actin promoter, albeit those of skill in the art can appreciate that any promoter capable of effecting expression in the intended host can be used in accordance with the methods of the present invention. The promoter may comprise a regulatable sequence such as the Tet operator sequence. Sequences such as these that offer the potential for regulation of transcription and expression are useful in instances where repression of gene transcription is sought. The adenoviral gene expression cassette may comprise a transcription termination sequence; specific embodiments of which are the bovine growth hormone termination/polyadenylation signal (bGHpA) or the short synthetic polyA signal (SPA) of 50 nucleotides in length defined as follows: AATAAAAGATCTTTATTTTCATTAGATCTGTGTGTT-GGTTTTTTGTGTG (SEQ ID NO:2). A leader or signal peptide may also be incorporated into the transgene. In specific embodiments, the leader is derived from the tissue-specific plasminogen activator protein, tPA.  
         [0035]    Heterologous nucleic acids of interest are genes (or their functional counterparts) which encode immunogenic and/or therapeutic proteins. Preferred therapeutic proteins are those which elicit some measurable therapeutic benefit in the individual host upon administration. Preferred immunogenic proteins are any proteins which are capable of eliciting an immune response in an individual. Applicants have exemplified the delivery of a representative immunogenic protein (HIV gag) in the present specification in non-human primates (rhesus macaques), albeit any gene encoding a therapeutic or immunogenic protein can be used in accordance with the methods disclosed herein. The adenovirus serotype 34 vectors were found to induce significant levels of gag-specific T cells; FIG. 5. Moreover, the results indicated that immunization with the disclosed vectors was able to elicit both HIV-specific CD4+ and CD8+ T cells; FIG. 6.  
         [0036]    An aspect of the present invention, therefore, relates to adenovirus serotype 34-based vectors carrying an HIV transgene. In these embodiments, nucleic acid encoding any HIV antigen may be utilized (specific examples of which include gag, pol, nef, gp160, gp41, gp120, tat, and rev, including derivatives of the aforementioned genes). The embodiments exemplified herein employ nucleic acid encoding a codon-optimized p55 gag antigen; see FIG. 7 (SEQ ID NO: 3). Codon-optimized HIV-1 env genes are disclosed in PCT International Applications PCT/US97/02294 and PCT/US97/10517, published Aug. 28, 1997 (WO 97/31115) and Dec. 24, 1997, respectively. Codon-optimized HIV-1 pol genes are disclosed in U.S. application Ser. No. 09/745,221, filed Dec. 21, 2000 and PCT International Application PCT/US00/34724, also filed Dec. 21, 2000. Codon-optimized HIV-1 nef genes are disclosed in U.S. application Ser. No. 09/738,782, filed Dec. 15, 2000 and PCT International Application PCT/US00/34162, also filed Dec. 15, 2000.  
         [0037]    In this specific embodiment of a recombinant, replication-defective Ad34 vector comprising an HIV-1 gene, the gene may be derived from HIV-1 strain CAM-1; Myers et al, eds. “Human Retroviruses and AIDS: 1995, IIA3-IIA19, which is hereby incorporated by reference. This gene closely resembles the consensus amino acid sequence for the lade B (North American/European) sequence. HIV gene sequence(s) may be based on various clades of HIV-1; specific examples of which are Clades B and C. Sequences for genes of many HIV strains are publicly available from GenBank and primary, field isolates of HIV are available from the National Institute of Allergy and Infectious Diseases (NIAID) which has contracted with Quality Biological (Gaithersburg, Md.) to make these strains available. Strains are also available from the World Health Organization (WHO), Geneva Switzerland. It is well within the purview of the skilled artisan to choose an appropriate nucleotide sequence which encodes a specific HIV antigen, or immunologically relevant portion or modification thereof. “Immunologically relevant” as defined herein means (1) with regard to a viral antigen, that the protein is capable, upon administration, of eliciting a measurable immune response within an individual sufficient to retard the propagation and/or spread of the virus and/or to reduce the viral load present within the individual; or (2) with regards to a nucleotide sequence, that the sequence is capable of encoding for a protein capable of the above.  
         [0038]    The present invention encompasses methods for (1) effectuating a therapeutic response in an individual and (2) generating an immune response (including a cellular-mediated immune response) comprising administering to an individual a recombinant adenovirus serotype 34 vector in accordance with the present invention. One aspect of the present invention are methods for generating an enhanced immune response against one or more antigens (bacterial, viral (e.g., HIV), or other (e.g., cancer)) which comprise the administration of a recombinant adenovirus serotype 34 vehicle expressing the antigen of interest. Administration of recombinant Ad34 vectors in this manner provides for improved cellular-mediated immune responses, particularly where there is pre-existing immunity in a given host to the more well-represented adenovirus serotypes (e.g., Ad2 and Ad5). An effect of the improved vaccine administration methods should be a lower transmission rate to (or occurrence rate in) previously uninfected individuals (i.e., prophylactic applications) and/or a reduction in the levels of virus/bacteria/foreign agent within an infected individual (i.e., therapeutic applications). As relates to HIV indications, an effect of the improved vaccine administration methods should be a lower transmission rate to previously uninfected individuals (i.e., prophylactic applications) and/or a reduction in the levels of viral loads within an infected individual (i.e., therapeutic applications) so as to prolong the asymptomatic phase of HIV infection. Administration, intracellular delivery and expression of the recombinant Ad34 vectors elicits a host CTL and Th response.  
         [0039]    Accordingly, the present invention relates to methodology regarding administration of the recombinant Ad34 viral vectors (or immunogenic compositions thereof, herein termed vaccines) to provide effective immunoprophylaxis, to prevent establishment of an infection following exposure to the viral (for instance, HIV), bacterial or other agent, or as a post-infection therapeutic vaccine to mitigate infection to result in the establishment of a lower virus/bacteria/other load with beneficial long term consequences.  
         [0040]    The recombinant adenovirus serotype 34 vectors of the present invention may be administered alone, or as part of a prime/boost administration regimen. A priming dose(s) of at least one antigen (e.g., an HIV antigen) is first delivered with a recombinant adenoviral vector. This dose effectively primes the immune response so that, upon subsequent identification of the antigen(s) in the circulating immune system, the immune response is capable of immediately recognizing and responding to the antigen(s) within the host. The priming dose(s) is then followed with a boosting dose comprising a recombinant adenoviral vector containing at least one gene encoding the antigen. A mixed modality prime and boost inoculation scheme will result in an enhanced immune response, particularly where there is pre-existing anti-vector immunity. Prime-boost administrations typically involve priming the subject (by viral vector, plasmid, protein, etc.) at least one time, allowing a predetermined length of time to pass, and then boosting (by viral vector, plasmid, protein, etc.). Multiple primings, typically 1-4, are usually employed, although more may be used. The length of time between priming and boost may typically vary from about four months to a year, albeit other time frames may be used as one of ordinary skill in the art will appreciate.  
         [0041]    In addition to a single protein or antigen of interest being delivered by the recombinant, replication-defective adenovirus serotype 34 vectors of the present invention, two or more proteins or antigens can be delivered either via separate vehicles or delivered via the same vehicle. Multiple genes/functional equivalents may be ligated into a proper shuttle plasmid for generation of a pre-adenoviral plasmid comprising multiple open reading frames. Open reading frames for the multiple genes/functional equivalents can be operatively linked to distinct promoters and transcription termination sequences. In other embodiments, the open reading frames may be operatively linked to a single promoter, with the open reading frames operatively linked by an internal ribosome entry sequence (IRES; as disclosed in WO 95/24485), or suitable alternative allowing for transcription of the multiple open reading frames to run off of a single promoter. In certain embodiments, the open reading frames may be fused together by stepwise PCR or suitable alternative methodology for fusing together two open reading frames. Due consideration must be given, however, to the effective packaging limitations of the viral vehicle. Adenovirus type 5, for instance, has been shown to exhibit an upper cloning capacity limit of approximately 105% of the wildtype Ad5 sequence.  
         [0042]    Prime-boost regimens can employ different adenoviral serotypes. One example of such a protocol would be a priming dose(s) comprising a recombinant adenoviral vector of a first serotype followed by a boosting dose comprising a recombinant adenoviral vector of a second and different serotype; see, for instance, Example 6 and FIGS. 12 and 13. Therein, a cohort of monkeys was given two doses of an Ad34-based HIV gag vector at weeks 0 and 4, and boosted at week 24 with an Ad35-based HIV gag vector. Administration of the Ad35-based vector resulted in about a 3-fold enhancement in T cell responses when compared to the levels at the time of the booster. In an alternative embodiment, the priming dose can comprise a mixture of separate adenoviral vehicles each comprising a gene encoding for a different protein/antigen. In such a case, the boosting dose would also comprise a mixture of vectors each comprising a gene encoding for a separate protein/antigen, provided that the boosting dose(s) administers recombinant viral vectors comprising genetic material encoding for the same or similar set of antigens that were delivered in the priming dose(s). These multiple gene/vector administration modalities can further be combined. It is further within the scope of the present invention to embark on combined modality regimes which include multiple but distinct components from a specific antigen.  
         [0043]    Compositions, including vaccine compositions, comprising the adenoviral vectors of the present invention are an important aspect of the present invention. These compositions can be administered to mammalian hosts, preferably human hosts, in either a prophylactic or therapeutic setting. Potential hosts/vaccinees include but are not limited to primates and especially humans and non-human primates, and include any non-human mammal of commercial or domestic veterinary importance. Compositions comprising recombinant adenoviral serotype 34 vectors may be administered alone or in combination with other viral- or non-viral-based DNA/protein vaccines. They also may be administered as part of a broader treatment regimen. The present invention encompasses those situations as well where the disclosed recombinant adenoviral serotype 34 vectors are administered in conjunction with other therapies; for example, HAART therapy (in the case of a recombinant HIV vector).  
         [0044]    Compositions comprising the recombinant viral vectors may contain physiologically acceptable components, such as buffer, normal saline or phosphate buffered saline, sucrose, other salts and polysorbate. In certain embodiments, the formulation has: 2.5-10 mM TRIS buffer, preferably about 5 mM TRIS buffer; 25-100 mM NaCl, preferably about 75 mM NaCl; 2.5-10% sucrose, preferably about 5% sucrose; 0.01-2 mM MgCl 2 ; and 0.001%-0.01% polysorbate 80 (plant derived). The pH should range from about 7.0-9.0, preferably about 8.0. One skilled in the art will appreciate that other conventional vaccine excipients may also be used in the formulation. In specific embodiments, the formulation contains 5mM TRIS, 75 mM NaCl, 5% sucrose, 1 mM MgCl 2 , 0.005% polysorbate 80 at pH 8.0. This has a pH and divalent cation composition which is near the optimum for Ad5 and Ad6 stability and minimizes the potential for adsorption of virus to a glass surface. It does not cause tissue irritation upon intramuscular injection. It is preferably frozen until use.  
         [0045]    The amount of viral particles in the vaccine composition to be introduced into a vaccine recipient will depend on the strength of the transcriptional and translational promoters used and on the immunogenicity of the expressed gene product. In general, an immunologically or prophylactically effective dose of 1×10 7  to 1×10 12  particles and preferably about 1×10 10  to 1×10 11  particles is administered directly into muscle tissue. Subcutaneous injection, intradermal introduction, impression through the skin, and other modes of administration such as intraperitoneal, intravenous, or inhalation delivery are also contemplated. Parenteral administration, such as intravenous, intramuscular, subcutaneous or other means of administration of interleukin-12 protein, concurrently with or subsequent to parenteral introduction of the vaccine compositions of this invention is also advantageous.  
         [0046]    The following non-limiting Examples are presented to better illustrate the workings of the invention.  
       EXAMPLE 1  
       [0047]    Construction of pAd34ΔE1ΔE4Ad5Orf6  
         [0048]    To generate an E1-Ad34 based vector that can propagate in existing group C/Ad5 E1 complementing cell lines (293, PER.C6), Ad5 Orf6 was inserted in place of the native E4 region. To construct the Ad34 pre-Adenovirus plasmid, advantage was taken of the sequence homology between Ad34 and Ad35. Cotransformation of BJ 5183 bacteria with purified wild-type Ad34 viral DNA and the appropriately constructed Ad35 ITR cassette resulted in the circularization of the viral genome by homologous recombination. The construction of the pre-Ad plasmid based on Ad34, is outlined below:  
         [0049]    To construct pAd34ΔE1ΔE4Ad5Orf6 (An Ad34 pre-Ad plasmid containing an E1 deletion and an E4 deletion substituted with Ad5 Orf6), we utilized an Ad35 ITR cassette. We anticipated that sequence homology between Ad34 and Ad35 would allow homologous recombination to occur. The Ad35 ITR cassette was constructed containing sequences from the right (bp 31599 to 31913 and bp 34419 to 34793) and left (bp 4 to 456 and bp 3403 to 3886) end of the Ad35 genome (see FIGS. 11A-1 to  11 A- 10 ) separated by plasmid sequences containing a bacterial origin of replication and an ampicillin resistance gene. The four segments were generated by PCR and cloned sequentially into pNEB193, generating pNEBAd35-4. Next the Ad5 Orf6 open reading frame was generated by PCR and cloned between Ad35 bp 31913 and 34419 generating pNEBAd35-4Ad5Orf6 (the ITR cassette). PNEB193 is a commonly used commercially available cloning plasmid (New England Biolabs cat# N3051S) containing a bacterial origin of replication, ampicillin resistance gene and a multiple cloning site into which the PCR products were introduced. The ITR cassette contains a deletion of E1 sequences from Ad35 bp 457 to 3402 with a unique Swa I restriction site located in the deletion and an E4 deletion from Ad35 bp 31914 to 34418 into which Ad5 Orf6 was introduced in an E4 parallel orientation. In this construct Ad5Orf6 expression is driven by the Ad35 E4 promoter. The Ad35 sequences (bp 31599 to 31913 and bp 3403 to 3886) in the ITR cassette provided regions of homology with the purified Ad34 viral DNA in which bacterial recombination could occur following cotransformation into BJ 5183 bacteria (FIG. 1). The ITR cassette was also designed to contain unique restriction enzyme sites (PmeI) located at the end of the viral ITR&#39;s so that digestion would release the recombinant Ad34 genome from the plasmid sequences. Potential clones were screened by restriction analysis and one clone was selected as pAd34ΔE1ΔE4Ad5Orf6.  
       EXAMPLE 2  
       [0050]    Rescue of pAd34ΔE1ΔE4Ad5Orf6 into Virus  
         [0051]    In order to determine if pre-adenovirus plasmid pAd34ΔE1ΔE4Ad5Orf6 could be rescued into virus and propagated in a group C E1 complementing cell line, the plasmid was digested with Pme I and transfected into T-25 flasks of PER.C6 cells using the calcium phosphate co-precipitation technique (Cell Phect Transfection Kit, Amersham Pharmacia Biotech Inc). PmeI digestion releases the viral genome from plasmid sequences allowing viral replication to occur after cell entry. Viral cytopathic effect (CPE), indicating that virus replication and amplification was occurring was observed following transfection. When CPE was complete, approximately 7-10 days post transfection, the infected cells and media were harvested, freeze/thawed three times and the cell debris pelleted by centrifugation. Approximately 1 ml of the cell lysate was used to infect a T-225 flask of PER.C6 cells at 80-90% confluence. Once CPE was reached, infected cells and media were harvested, freeze/thawed three times and the cell debris pelleted by centrifugation. Clarified cell lysates were then used to infect 2-layer NUNC cell factories of PER.C6 cells. Following complete CPE, the virus was purified by ultracentrifugation on CsCl density gradients. In order to verify the genetic structure of the rescued viruses, viral DNA was extracted using pronase treatment followed by phenol chloroform extraction and ethanol precipitation. Viral DNA was then digested with HindIII and treated with Klenow fragment to end-label the restriction fragments with P33-dATP. The end-labeled restriction fragments were then size-fractionated by gel electrophoresis and visualized by autoradiography. The digestion products were compared with the digestion products of the corresponding pre-Adenovirus plasmid (that had been digested with Pme1/HindIII prior to labeling) from which they were derived. The expected sizes were observed, indicating that the viruses had been successfully rescued.  
       EXAMPLE 3  
       [0052]    Insertion of an Expression Cassette into pAd34ΔE1ΔE4Ad5Orf6  
         [0053]    In order to introduce a gag or SEAP expression cassette (see FIGS. 8 and 9, respectively) into the E1 region of pAd34ΔE1ΔE4Ad5Orf6 bacterial recombination was again used. A gag expression cassette consisting of the following: 1) the immediate early gene promoter from human cytomegalovirus, 2) the coding sequence of the human immunodeficiency virus type 1 (HIV-1) gag (strain CAM-1; 1526 bp) gene, and 3) the bovine growth hormone polyadenylation signal sequence, was cloned into the E1 deletion in Ad35 shuttle plasmid, pNEBAd35-2 (a precursor to the Ad35 ITR cassettes described above), generating pNEBAd35CMVgagBGHpA. pNEBAd35-2 contains Ad35 sequences from the left end of the genome (bp 4 to 456 and bp 3403 to 3886) with a unique SwaI site between bp 456 and 3403 at the position of the deletion. The gag expression cassette was obtained from a previously constructed shuttle plasmid by EcoRI digestion. Following the digestion the desired fragment was gel purified, treated with Klenow to obtain blunt ends and cloned into the SwaI site in pNEBAd35-2. This cloning step resulted in the gag expression cassette being inserted into the E1 deletion between bp 456 and 3403 in the E1 parallel orientation. The shuttle vector containing the gag transgene was digested to generate a DNA fragment consisting of the gag expression cassette flanked by Ad35 bp 4 to 456 and bp 3403 to 3886 and the fragment was purified after electrophoresis on an agarose gel. Cotransformation of BJ 5183 bacteria with the shuttle vector fragment and pAd34ΔE1ΔE4Ad5Orf6 linearized in the E1 region by digestion with Swa I, resulted in the generation of the Ad34 gag-containing pre-Adenovirus plasmid pAd34ΔE1ΔE4Ad5Orf6 by homologous recombination. Potential clones were screened by restriction analysis.  
         [0054]    A similar strategy was used to generate Ad34 pre-Ad plasmids containing a SEAP expression cassette. In this case a SEAP expression cassette consisting of: 1) the immediate early gene promoter from human cytomegalovirus, 2) the coding sequence of the human placental SEAP gene, and 3) the bovine growth hormone polyadenylation signal sequence was cloned into the E1 deletion in Ad35 shuttle plasmid, pNEBAd35-2, generating pNEBAd35CMVSEAPBGHpA. The SEAP expression cassette was obtained from a previously constructed shuttle plasmid by EcoRI digestion. Following the digestion the desired fragment was gel purified, treated with Klenow to obtain blunt ends and cloned into the SwaI site in pNEBAd35-2. The transgene was then recombined into the pAd34ΔE1ΔE4Ad5Orf6, generating pAd34ΔE1ΔE4Ad5Orf6 as described above for the gag transgene.  
         [0055]    All pre-Ad plasmids were rescued into virus and expanded to prepare CsCl purified stocks as described above.  
       EXAMPLE 4  
       [0056]    Construction of pMRKAd34ΔE1ΔE4Ad5Orf6  
         [0057]    To construct an Ad34 pre-Ad plasmid that was composed entirely of Ad34 sequences, an Ad34 ITR cassette was generated. The Ad34 ITR cassette was constructed containing sequences from the right (bp 31584 to 31895 and bp 34409 to 34772) and left (bp 4 to 456 and bp 3402 to 3885) end of the Ad34 genome (see FIGS. 3A-1 to  3 A- 9 ) separated by plasmid sequences containing a bacterial origin of replication and an ampicillin resistance gene. These four segments were generated by PCR and cloned sequentially into pNEB193, generating pNEBAd34-4. Next the Ad5 Orf6 open reading frame was generated by PCR and cloned between Ad34 bp 31895 and 34409 generating pNEBAd34-4Ad5Orf6 (the ITR cassette). PNEB193 is a commonly used commercially available cloning plasmid (New England Biolabs cat# N3051S) containing a bacterial origin of replication, ampicillin resistance gene and a multiple cloning site into which the PCR products were introduced. The ITR cassette contains a deletion of E1 sequences from Ad34 bp 457 to 3401 with a unique Swa I restriction site located in the deletion and an E4 deletion from Ad34 bp 31896 to 34408 into which Ad5 Orf6 was introduced in an E4 parallel orientation. In this construct Ad5Orf6 expression is driven by the Ad34 E4 promoter. The Ad34 sequences (bp 31584 to 31895 and bp 3402 to 3885) in the ITR cassette provided regions of homology with the purified Ad34 viral DNA in which bacterial recombination could occur following cotransformation into BJ 5183 bacteria (FIG. 2). The ITR cassette was also designed to contain unique restriction enzyme sites (PmeI) located at the end of the viral ITR&#39;s so that digestion would release the recombinant Ad34 genome from the plasmid sequences. Potential clones were screened by restriction analysis and one clone was selected as pMRKAd34ΔE1ΔE4Ad5Orf6.  
       EXAMPLE 5  
       [0058]    In Vivo Studies  
         [0059]    A. Immunization  
         [0060]    Cohorts of 3 rhesus macaques were given single intramuscular injections of one of the two vectors: (1) 10{circumflex over ( )}11 vp MRKAd5-SEAP (in MRKAd vector backbone of FIGS. 10A-1 to  10 A- 45  disclosed in PCT/US01/28861, published Mar. 21, 2002); and (2) 10{circumflex over ( )}11 vp Ad34ΔE1SEAPΔE4Ad5Orf6. Rhesus macaques were between 3-10 kg in weight. In all cases, the total dose of each vaccine was suspended in 1 mL of buffer. The macaques were anesthetized (ketamine/xylazine) and the vaccines were delivered i.m. in 0.5-mL aliquots into both deltoid muscles using tuberculin syringes (Becton-Dickinson, Franklin Lakes, N.J.). Peripheral blood mononuclear cells (PBMC) were prepared from blood samples collected at several time points during the immunization regimen. All animal care and treatment were in accordance with standards approved by the Institutional Animal Care and Use Committee according to the principles set forth in the  Guidefor Care and Use of Laboratory Animals , Institute of Laboratory Animal Resources, National Research Council.  
         [0061]    B. SEAP Assay  
         [0062]    Serum samples were analyzed for circulating human secreted alkaline phosphatase (SEAP) levels using TROPIX phospha-light chemiluminescent kit (Applied Biosystems Inc). Duplicate 5 μL aliquots of each serum were mixed with 45 μL of kit-supplied dilution buffer in a 96-well white DYNEX plate. Serially diluted solutions of a human placental alkaline phosphatase (Catalog no. M5905, Sigma, St. Louis, Mo.) in 10% naive monkey serum served to provide the standard curve. Endogenous SEAP activity in the samples was inactivated by heating the well for 30 minutes at 65° C. Enzymatic SEAP activities in the samples were determined following the procedures described in the kit. Chemiluminescence readings (in relative light units) were recorded using DYNEX luminometer. RLU readings were converted to ng/mL SEAP using a log-log regression analyses.  
         [0063]    C. ELISPOT Assay  
         [0064]    The IFN-γ ELISPOT assays for rhesus macaques were conducted following a previously described protocol (Allen et al., 2001  J. Virol.  75(2):738-749), with some modifications. For antigen-specific stimulation, a peptide pool was prepared from 20-aa peptides that encompass the entire HIV-1 gag sequence with 10-aa overlaps (Synpep Corp., Dublin, Calif.). To each well, 50 μL of 2-4×10 5  peripheral blood mononuclear cells (PBMCs) were added; the cells were counted using Beckman Coulter Z2 particle analyzer with a lower size cut-off set at 80 femtoliters (“fL”). Either 50 μL of media or the gag peptide pool at 8 μg/mL concentration per peptide were added to the PBMC. The samples were incubated at 37° C., 5% CO 2  for 20-24 hrs. Spots were developed accordingly and the plates were processed using custom-built imager and automatic counting subroutine based on the ImagePro platform (Silver Spring, Md.); the counts were normalized to 10 6  cell input.  
         [0065]    D. Intracellular Cytokine Staining (ICS)  
         [0066]    To 1 ml of 2×10 6  PBMC/mL in complete RPMI media (in 17×100 mm round bottom polypropylene tubes (Sarstedt, Newton, N.C.)), anti-hCD28 (clone L293, Becton-Dickinson) and anti-hCD49d (clone L25, Becton-Dickinson) monoclonal antibodies were added to a final concentration of 1 μg/mL. For gag-specific stimulation, 10 μL of the peptide pool (at 0.4 mg/mL per peptide) were added. The tubes were incubated at 37° C. for 1 hr., after which 20 μL of 5 mg/mL of brefeldin A (Sigma) were added. The cells were incubated for 16 hr at 37° C., 5% CO 2 , 90% humidity. 4 mL cold PBS/2% FBS were added to each tube and the cells were pelleted for 10 min at 1200 rpm. The cells were re-suspended in PBS/2% FBS and stained (30 min, 4° C.) for surface markers using several fluorescent-tagged mAbs: 20 μL per tube anti-hCD3-APC, clone FN-18 (Biosource); 20 μL anti-hCD8-PerCP, clone SK1 (Becton Dickinson); and 20 μL anti-hCD4-PE, clone SK3 (Becton Dickinson). Sample handling from this stage was conducted in the dark. The cells were washed and incubated in 750 μL 1×FACS Perm buffer (Becton Dickinson) for 10 min at room temperature. The cells were pelleted and re-suspended in PBS/2% FBS and 0.1 μg of FITC-anti-hIFN-γ, clone MD-1 (Biosource) was added. After 30 min incubation, the cells were washed and re-suspended in PBS. Samples were analyzed using all four color channels of the Becton Dickinson FACSCalibur instrument. To analyze the data, the low side- and forward-scatter lymphocyte population was initially gated; a common fluorescence cut-off for cytokine-positive events was used for both CD4 +  and CD8 +  populations, and for both mock and gag-peptide reaction tubes of a sample.  
         [0067]    E. Results  
         [0068]    Expression: Serum samples prior to and after the injection were analyzed for circulating SEAP activities and the results are shown in FIG. 4. Results indicate that the peak levels of SEAP protein produced by the alternative adenovirus serotype were lower than but were within 3-fold of that of MRKAd5 at the same high dose level of 10{circumflex over ( )}11 vp (FIG. 4). The levels of SEAP in the serum dropped dramatically after day 10 and were close to background as early as day 15. These observations strongly indicate that the Ad34-based vector is efficient in expressing a transgene following intramuscular administration in a primate.  
         [0069]    Immunogenicity: Vaccine-induced T cell responses against HIV-1 gag were quantified using IFN-gamma ELISPOT assay against a pool of 20-aa peptides that encompassed the entire protein sequence. The results are shown in FIG. 5; they are expressed as the number of spot-forming cells (SFC) per million peripheral blood mononuclear cells (PBMCs) that responded to the peptide pool or the mock (no peptide) control.  
         [0070]    Immunization with gag-expressing Ad34 vector induced detectable levels of circulating gag-specific T cells immediately after a single dose of the vector. The responses improved following a second dose given at wk 4. Overall, the responses to the Ad34-based vector were slightly lower than those induced by the same dose of MRKAd5-gag. The results strongly indicate the Ad34-based vector can prime effectively for HIV-specific T cell responses.  
         [0071]    IFN-γ ICS analyses of the PBMC from the Ad34-immunized animals revealed that the vector can induce detectable levels of both CD4 +  and CD8 +  HIV-specific T cells (FIG. 6).  
       EXAMPLE 6  
       [0072]    Heterologous Immunization  
         [0073]    Cohorts of 3 monkeys were immunized (at wks 0, 4) with 10{circumflex over ( )}11 vp Ad34ΔE1gagΔE4Ad5Orf6 followed by a booster at week 24 with 10{circumflex over ( )}10 vp Ad35ΔE1gagΔE4Ad5Orf6. Vaccine-induced T cell responses against HIV-1 gag were quantified using IFN-gamma ELISPOT assay against a pool of 20-aa peptides that encompassed the entire protein sequence. The results are shown in FIG. 12; they are expressed as the number of spot-forming cells (SFC) per million peripheral blood mononuclear cells (PBMCs) that responded to the peptide pool or the mock (no peptide) control.  
         [0074]    Immunization with gag-expressing Ad34 vector induced detectable levels of circulating gag-specific T cells that decreased to between 94-139 SFC/10{circumflex over ( )}6 PBMC at the time of the boost. Heterologous immunization with an Ad35-based HIV vector resulted in as much as a 3-fold increase in T cell responses.  
         [0075]    IFN- 65  ICS analyses of the PBMCs from the Ad34 primed/Ad35 boosted animals at week 28 revealed that the vector can induce detectable levels of both CD4 +  and CD8 +  HIV-specific T cells (FIG. 13).  
     
       
       
         1 
         
           
             13  
           
           
             1  
             34775  
             DNA  
             adenovirus serotype 34  
           
            1 

catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtgatttta     60 

aaaattgtgg ggtgtgtggt gattggctgt ggggttaacg gctaaacggg gcggcgcggc    120 

cgtgggaaaa tgacgttttg tgggggtgga gtttttttgc aagttgtcgc gggaaatgtg    180 

acgcataaaa aggctttttt tctcacggaa ctactgactt ttcccacggt atttaacagg    240 

aaatgaggta gttttgaccg gatgcaagtg aaaattgctg atttgcgcgc gaaaactgaa    300 

tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg    360 

ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt    420 

ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctacggtatt    480 

tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc    540 

tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat    600 

aatttctgct gagactggaa atgaaatact ggagcttgtg gtgcacgccc tgatgggaga    660 

cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt    720 

agaggtagag ggatcggagg attctaatga ggaagctgtg aatggctttt ttaccgattc    780 

tatgctttta gctgctaatg aaggattaga attagatccg cctttggaca ctttcgatac    840 

tccaggggtg attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgggttccgt    900 

ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga    960 

aaaggagcag tctatgcaga ctgcagcggg tgagggagtg aaggctgcca gtgttggttt   1020 

tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa   1080 

aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagagcgc actgccactt   1140 

tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat   1200 

attgagtggg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgagtcacc   1260 

atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg   1320 

caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga   1380 

cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacggc caagacaata   1440 

agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaga gtgcaatgta   1500 

ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata   1560 

taagtagaag cagacctgta tggttagctc ataggagctg gctttcatcc atggaggttt   1620 

gggccatttt ggaagacctt agaaagacta ggcaactgtt agaggacgct tcggacggag   1680 

tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa   1740 

aacaggacta taaagaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag   1800 

ctcttaattt gggccatcaa gttcacttta aagaaaaagt tttatcagtt ttagactttt   1860 

caaccccagg tagaactgcc gctgctgtgg cttttcttac ttttatatta gataaatgga   1920 

tcccgcagac tcatttcagc aggggatacg ttttggattt cgtagccaca gcattgtgga   1980 

gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg   2040 

gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc   2100 

aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt   2160 

gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt   2220 

taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat   2280 

gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtc cagaaagagg gaagggatga   2340 

agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc   2400 

tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa   2460 

acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg   2520 

ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat   2580 

gtggcctgga gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga   2640 

tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt   2700 

ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag   2760 

tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa   2820 

atgcatattc caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca   2880 

ctgcgcttct acagatactg gatgttttat tttaattaag ggcaatgcca gcgtaaagca   2940 

taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgccggtgg   3000 

gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt   3060 

ttttgatcac aatgtgttga ccaagtgtac catgcatgca ggtgggcgta gaggaatgtt   3120 

tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc   3180 

cagaatgagc ctaacaggaa tctttgacat gaacatgcaa atctggaaga tcctgaggta   3240 

tgatgatacg agatcgaggg tgcgcgcatg cgaatgcgga ggcaagcatg ccaggttcca   3300 

gccggtgtgt gtagatgtga ctgaagatct gagaccggat catttggtta ttgcccgcac   3360 

tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt   3420 

ggggtggggt tttcagatgg acagattgag taaaaatttg ttttttctgt ctttcagctg   3480 

tcatgagtgg aaacgcttct tttaaggggg gagtcttcag cccttatctg acagggcgtc   3540 

tcccatcctg ggcaggagtt cgtcagaatg ttatgggatc tactgtggat ggaagacccg   3600 

tccaacccgc caattcttca acgctgacct atgctacttt aagttcttca cctttggacg   3660 

cagctgcagc cgccgccgcc gcctctgttg ccgctaacac tgtgcttgga atgggttact   3720 

atggaagtat cgtggctaat tccacttcct ctaataaccc ttctaccctg actcaggaca   3780 

agttacttgt ccttttggcc cagctggagg ctttgaccca acgtctgggt gaactttatc   3840 

agcaggtggc cgagttgcga gtacaaactg agtctgctgt cggcacggca aagtctaaat   3900 

aaaaaaaaat tccacaatca atgaataaat aaacgagctt gttgttgatt taaaatcaag   3960 

tgtttttatt tcatttttcg cgcacggtat gccctagacc accgatctcg atcattgaga   4020 

acacggtgga ttttttccag aatcctatag aggtgggatt gaatgtttag atacatgggc   4080 

attaggccat ctttggggtg gagatagctc cattgaaggg attcatgctc cggggtagtg   4140 

ttgtaaatca cccagtcata acaaggtcgc agtgcatggt gttgcacaat atcttttaga   4200 

agtaggctga ttgccacaga taagcccttg gtgtaggtgt ttacaaaccg gttgagctgg   4260 

gaggggtgca ttcggggtga aattatgtgc attttggatt ggatttttaa gttggcaata   4320 

ttgccgccaa gatctcgtct tgggttcatg ttatgaagga ccaccaagac ggtgtatccg   4380 

gtacatttag gaaatttatc gtgtagcttg gatggaaaag cgtggaaaaa tttggagaca   4440 

cccttgtgtc ctccgagatt ttccatgcac tcatccatga taatagcaat ggggccgtgg   4500 

gcagcagcgc gggcaaacac gttccgtggg tctgacacat catagttatg ttcctgagtt   4560 

aaatcatcat aagccatttt aatgaatttg gggcggagag tacccgattg gggtatgaat   4620 

gttccttcgg gccccggagc atagttcccc tcacagattt gcatttccca agctttcagt   4680 

tccgatggtg gaatcatgtc cacctggggg gctatgaaga acaccgtttc tggggcgggg   4740 

gtgattagtt gggatgatag caagtttctg agcaattgag atttgccaca tccggtgggg   4800 

ccataaatga ttccgattac aggttgcagg tggtagttta gggaacggca actgccgtct   4860 

tctcgaagca agggggccac ctcgttcatc atttccctta catgcatatt ttcccgcacc   4920 

aaatccatta ggaggcgctc tcctcctagt gatagaagtt cttgtagtga ggaaaagttt   4980 

ttcagcggtt ttagaccgtc agccatgggc attttggaga gagtttgctg caaaagttct   5040 

agtctgttcc acagttcagt gatgtgttct atggcatctc gatccagcag acctcctcgt   5100 

ttcgcgggtt tggacggctc ctggagtagg gtatgagacg atgggcgtcc agcgctgcca   5160 

gggttcggtc cttccagggt ctcagtgttc gagtcagggt tgtttccgtc acagtgaagg   5220 

ggtgtgcgcc tgcttgggcg cttgccaggg tgcgcttcag actcattctg ctggtggaga   5280 

acttctgtcg cttggcgccc tgtatgtcgg ccaagtagca gtttaccatg agttcgtagt   5340 

tgagcgcctc ggctgcgtgg cctttggcgc ggagcttacc tttggaagtt ttcttgcata   5400 

ccgggcagta taggcatttc agcgcataca gcttgggcgc aaggaaaatg gattctgggg   5460 

agtatgcatc tgcgccgcag gaggcgcaaa cagtttcaca ttccaccagc caggttaaat   5520 

ccggttcatt ggggtcaaaa acaagttttc cgccatattt tttgatgcgt ttcttacctt   5580 

tggtctccat gagttcgtgt cctcgttgag tgacaaacag gctgtccgta tccccgtaga   5640 

ctgattttac aggcctcttc tccagtggag tgcctcggtc ttcttcgtac aggaactctg   5700 

accactctga tacaaaggcg cgcgtccagg ccagcacaaa ggaggctatg tgggaggggt   5760 

agcgatcgtt gtcaaccagg gggtccacct tttccaaagt atgcaaacac atgtcaccct   5820 

cttcaacatc caggaatgtg attggcttgt aggtgtattt cacgtgacct ggggtccccg   5880 

ctgggggggt ataaaagggg gcggttcttt gctcttcctc actgtcttcc ggatcgctgt   5940 

ccaggaacgt cagctgttgg ggtaggtatt ccctctcgaa ggcgggcatg acctctgcac   6000 

tcaggttgtc agtttctaag aacgaggagg atttgatatt gacagtgccg gttgagatgc   6060 

ctttcatgag gttttcgtcc atttggtcag aaaacacaat ttttttattg tcaagtttgg   6120 

tggcaaatga tccatacagg gcgttggata aaagtttggc aatggatcgc atggtttggt   6180 

tcttttcctt gtccgcgcgc tctttggcag cgatgttgag ttggacatac tcgcgtgcta   6240 

ggcacttcca ttcggggaag atagttgtca attcatctgg cacgattctc acttgccacc   6300 

ctcgattatg caaggtaatt aaatccacac tggtggccac ctcgcctcga aggggttcgt   6360 

tggtccaaca gagcctacct cctttcctag aacagaaagg gggaagtggg tctagcataa   6420 

gttcatcggg agggtctgca tccatggtaa agattcccgg aagtaaatcc ttatcaaaat   6480 

agctgatggg agtggggtca tctaaggcca tttgccattc tcgagctgcc agtgcacgct   6540 

catatgggtt aaggggactg ccccagggca tgggatgggt gagtgcagag gcatacatgc   6600 

cacagatgtc atagacgtag atgggatcct caaagatgcc tatataggtt ggatagcatc   6660 

gcccccctct gatacttgct cgcacatagt catatagttc atgtgatggc gctagcaacc   6720 

ccggacccaa gttggtgcga ttgggttttt ctgttctgta gacaatctgg cgaaagatgg   6780 

cgtgagaatt ggaagagatg gtgggtcttt gaaaaatgtt gaaatgggca tgaggtagac   6840 

ctacagagtc tctgacaaag tgggcataag attcttgaag cttggttacc agttcggcgg   6900 

tgacaagtac gtctagggcg cagtagtcaa gtgtttcttg aatgatgtca taacctggtt   6960 

ggtttttctt ttcccacagt tcgcggttga gaaggtattc ttcgcgatcc ttccagtact   7020 

cttctagcgg aaacccgtct ttgtctgcac ggtaagatcc tagcatgtag aactgattaa   7080 

ctgccttgta agggcagcag cccttctcta cgggtagaga gtatgcttga gcagcttttc   7140 

gcagcgaagc gtgagtaagg gcgaaggtgt ctctgaccat gactttgaga aattggtatt   7200 

tgaagtccat gtcgtcacag gctccctgtt cccagagttg gaagtctacc cgtttcttgt   7260 

aggcggggtt gggcaaagcg aaagtaacat cgttgaagag aatcttaccg gctctgggca   7320 

taaaattgcg agtgatgcgg aaaggctgtg gtacttccgc tcgattgttg atcacctggg   7380 

cagctaggac gatctcgtcg aaaccgttga tgttgtgtcc tacgatgtat aattctatga   7440 

aacgcggcgt gcctttgacg tgaggtagct tattgagctc atcaaaggtt aggtctgtag   7500 

ggtcagataa ggcgtagtgt tcgagagccc attcgtgcag gtgaggattt gcatgtagga   7560 

atgatgacca aagatccacc gccagtgctg tttgtaactg gtcccgatac tgacgaaaat   7620 

gctggccaat tgccattttt tctggagtga cacagtagaa ggttctgggg tcttgttgcc   7680 

atcgatccca ctttagttta atggctagat cgtgggccat gttgacgaga cgctcttctc   7740 

ctgagagttt catgaccagc atgaaaggaa ctagttgttt gccaaaggac cccatccagg   7800 

tgtaagtttc cacatcgtag gtcaggaaga gtctttctgt gcgaggatga gagccgatcg   7860 

ggaagaactg gatttcctgc caccagttgg aggattggct gttgatgtga tggaagtaga   7920 

agtttctgcg gcgcgccgag cattcgtgtt tgtgcttgta cagacggccg cagtagtcgc   7980 

agcgttgcac gggttgtatc tcgtgaatga gctgtacctg gcttcccttg acgagaaatt   8040 

tcagtgggaa gccgaggcct ggcgattgta tctcgtgctc ttctatattc gctgtatcgg   8100 

cctgttcatc ttctgtttcg gtggtggtca tgctgacgag cccccgcggg aggcaagtcc   8160 

agacctcggc gcgggagggg cggagctgaa ggaccagagc gcgcaggctg gagctgtcca   8220 

gagtcctgag acgctgcgga ctcaggttag taggtaggga cagaagatta acttgcatga   8280 

tcttttccag ggcgtgcggg aggttcagat ggtacttgat ttccacaggt tcgtttgtag   8340 

agatgtcaat ggcttgcagg gttccgtgtc ctttgggcgc cactaccgta cctttgtttt   8400 

ttcttttgat cggtggtggc tctcttgctt cttgcatgct cagaagcgat gacggggacg   8460 

cgcgccgggc ggaagcggtt gttccggacc cggaggcatg gctggtagtg gcacgtcggc   8520 

gccgcgcacg ggcaggttct ggtactgcgc tctgagaaga cttgcgtgcg ccaccacgcg   8580 

tcgattgacg tcttgtatct gacgtctctg ggtgaaagct accggccccg tgagcttgaa   8640 

cctgaaagag agttcaacag aatcaatttc ggtatcgtta acggcagctt gtctcagtat   8700 

ttcttgtacg tcaccagagt tgtcctggta ggcgatctcc gccatgaact gctcgatttc   8760 

ttcctcctga agatctccgc gacccgctct ctcgacggtg gccgcgaggt cattggagat   8820 

acggcccatg agttgggaga atgcagtcat gcccgcctcg ttccagacgc ggctgtaaac   8880 

cacggccccc tcggagtctc ttgcgcgcat caccacctga gcgaggttaa gctccacgtg   8940 

tctggtgaag accgcatagt tgcataggcg ctgaaaaagg tagttgagtg tggtggcaat   9000 

gtgttcggcg acgaagaaat acatgatcca tcgtctcagc ggcatttcgc tgacatcgcc   9060 

cagagcttcc aagcgctcca tggcctcgta gaagtccacg gcaaaattaa aaaactggga   9120 

gtttcgcgcg gacacggtca attcctcctc gagaagacgg atgagttcgg ctatggtggc   9180 

ccgtacttcg cgttcgaagg ctcccgggat ctcttcttcc tcttctatct cttcttccac   9240 

taacatctct tcttcgtctt caggcggggg cggagggggc acacggcgac gtcgacggcg   9300 

cacgggcaaa cggtcgatga atcgttcaat gacctctccg cggcggcggc gcatggtttc   9360 

agtgacggcg cggccgttct cgcgcggtcg cagagtaaaa acaccgccgc gcatctcctt   9420 

aaagtggtga ctgggaggtt ctccgtttgg gagggagagg gcgctgatta tacattttat   9480 

taattggccc gtagggactg cgcgcagaga tctgatcgtg tcaagatcca cgggatctga   9540 

aaacctttcg acgaaagcgt ctaaccagtc acagtcacaa ggtaggctga gtacggcttc   9600 

ttgtgggcgg gggtggttat gtgttcggtc tgggtcttct gtttcttctt catctcggga   9660 

aggtgagacg atgctgctgg tgatgaaatt aaagtaggca gttctaagac ggcggatggt   9720 

ggcgaggagc accaggtctt tgggtccggc ttgctggata cgcaggcgat tggccattcc   9780 

ccaagcatta tcctgacatc tagcaagatc tttgtagtag tcttgcatga gccgttctac   9840 

gggcacttct tcctcacccg ttctgccatg catacgtgtg agtccaaacc cgcgcattgg   9900 

ttgtaccagt gccaagtcag ctacgactct ttcggcgagg atggcttgct gtacttgggt   9960 

gagggtggct tgaaagtcat caaaatccac aaagcggtgg taagccccgg tattaatggt  10020 

gtaagcacag ttggccatga ctgaccagtt aactgtctgg tgaccagggc gcacgagctc  10080 

ggtgtattta aggcgcgaat aggcgcgggt gtcaaagatg taatcgttgc aggtgcgcac  10140 

cagatactgg taacctataa gaaaatgcgg cggtggttgg cggtagagag gccatcgttc  10200 

tgtagctgga gcgccggggg cgaggtcttc caacataagg cggtgatagc cgtagatgta  10260 

cctggacatc caggtgattc ctgcggcggt agtagaagcc cgaggaaact cgcgtacgcg  10320 

gttccaaatg ttgcgtagcg gcatgaagta gttcattgta ggcacggttt gaccagtgag  10380 

gcgcgcgcag tcattgatgc tctatagaca cggagaaaat gaaagcgttc agcgactcga  10440 

ctccgtagcc tggaggaacg tgaacgggtt gggtcgcggt gtaccccggt tcgagacttg  10500 

tactcgagcc ggccggagcc gcggctaacg tggtattggc actcccgtct cgacccagcc  10560 

tacaaaaatc caggatacgg aatcgagtcg ttttgctggt tgccgaatgg cagggaagtg  10620 

agtcctattt tttttttttg ccgctcagat gcatcccgtg ctgcgacaga tgcgtcccca  10680 

acaacagccc ccctcgcagc agcagcaacc acaaaaggct gtccctgcaa ctactgcaac  10740 

tgccgctgtg agcggtgcgg gacagcccgc ctatgatctg gacttggaag agggcgaagg  10800 

actggcacgt ctaggtgcgc cttcgcccga gcggcatccg cgagttcaac tgaaaaaaga  10860 

ttctcgcgag gcgtatgtgc cccaacagaa cctatttaga gacagaagcg gcgaggagcc  10920 

ggaggagatg cgagcttccc gctttaacgc gggtcgtgag ctgcgtcacg gtttggacag  10980 

aagacgagtg ttgcgggacg aggatttcga agttgatgaa gtgacaggga tcagtcctgc  11040 

cagggcacac gtggctgcag ccaaccttgt atcggcttac gaacagacag taaaggaaga  11100 

gcgtaatttc caaaagtctt ttaataatca tgtgcgaacc ctcattgccc gcgaagaagt  11160 

cacccttggt ttgatgcatt tgtgggattt gatggaagct atcattcaga accctactag  11220 

caaacctctg accgcacagc tgtttctggt ggtgcaacac agcagagaca atgaggcttt  11280 

cagagaggcg ctgctcaaca tcaccgaacc cgaggggaga tggttgtatg atcttatcaa  11340 

cattctacag agtatcatag tgcaggagcg gagcctgggc ctggccgaga aggtggctgc  11400 

catcaattac tcggttttga gcttgggaaa gtattacgct cgcaagatct acaagactcc  11460 

atacgttccc atagacaagg aggtgaagat agatgggttc tacatgcgca tgacgctgaa  11520 

ggtgttgacc ctgagcgatg atcttggggt gtaccgcaat gacagaatgc atcgcgcggt  11580 

gagcgccagc aggaggcgcg agttaagcga cagggaactg atgcacagtt tgcaaagagc  11640 

tctaactgga gctggaaccg agggtgagaa ttactttgat atgggagctg acttgcagtg  11700 

gcagcctagt cgcagggctc tgaacgccgc gacggcagga tgtgagcttc cttacataga  11760 

agaggcggat gaaggcgagg aggaagaggg cgagtacttg gaagactgat ggcacaaccc  11820 

gtgttttttg ctagatggaa cagcaagcac cggatcccgc aatgcgggcg gcgctgcaga  11880 

gccagccgtc cggcattaac tcctcggacg attggaccca ggccatgcaa cgtatcatgg  11940 

cgttgacgac tcgcaacccc gaagccttta gacagcaacc ccaggccaac cgtctatcgg  12000 

ccatcatgga agctgtagtg ccttcccgct ctaatcccac tcatgagaag gtcctggcca  12060 

tcgtgaacgc gttggtggag aacaaagcta ttcgtccaga tgaggccgga ctggtataca  12120 

acgctctctt agaacgcgtg gctcgctaca acagtagcaa tgtgcaaacc aatttggacc  12180 

gtatgataac agatgtacgc gaagccgtgt ctcagcgcga aaggttccag cgcgatgcca  12240 

acctgggttc gctggtggcg ttaaatgctt tcttgagtac tcagcctgct aatgtgccgc  12300 

gtggtcaaca ggattatact aactttttaa gtgctttgag actgatggta tcagaagtac  12360 

ctcagagcga agtatatcag tccggtcctg attacttctt tcagactagc agacagggct  12420 

tgcagacggt aaatctgagc caagctttta aaaaccttaa aggtttgtgg ggagtgcatg  12480 

ccccggtagg agaaagagca accgtgtcta gcttgttaac tccgaactcc cgcctattat  12540 

tactgttggt agctcctttc accgacagcg gtagcatcga ccgtaattcc tatttgggtt  12600 

acctactaaa cctgtatcgc gaagccatag ggcaaagtca ggtggacgag cagacctatc  12660 

aagaaattac ccaagtcagt cgcgctttgg gacaggaaga cactggcagt ttggaagcca  12720 

ctctgaactt cttgcttacc aatcggtctc aaaagatccc tcctcaatat gctcttactg  12780 

cggaggagga gaggatcctt agatatgtgc agcagagcgt gggattgttt ctgatgcaag  12840 

agggggcaac tccgactgca gcactggaca tgacagcgcg aaatatggag cccagcatgt  12900 

atgccagtaa ccgacctttc attaacaaac tgctggacta cttgcacaga gctgccgcta  12960 

tgaactctga ttatttcacc aatgccatct taaacccgca ctggctgccc ccacctggtt  13020 

tctacacggg cgaatatgac atgcccgacc ctaatgacgg atttctgtgg gacgacgtgg  13080 

acagcgatgt tttttcacct ctttctgatc atcgcacgtg gaaaaaggaa ggcggcgata  13140 

gaatgcattc ttctgcatcg ctgtccgggg tcattggtgc taccgcggct gagcccgagt  13200 

ctgcaagtcc ttttcctagt ctaccctttt ctctacacag tgtacgtagc agcgaagtgg  13260 

gtagaataag tcgcccgagt ttaatgggcg aagaggagta cctaaacgat tccttgctca  13320 

gaccggcaag agaaaaaaat ttcccaaaca atggaataga aagtttggtg gataaaatga  13380 

gtagatggaa gacttatgct caggatcaca gagacgagcc tgggatcatg gggactacaa  13440 

gtagagcgag ccgtagacgc cagcgccatg acagacagag gggtcttgtg tgggacgatg  13500 

aggattcggc cgatgatagc agcgtattgg acttgggtgg gagaggaagg ggcaacccgt  13560 

ttgctcattt gcgccctcgc ttgggtggta tgttgtaaaa aaaaataaaa aagaaaaaac  13620 

tcaccaaggc catggcgacg agcgtacgtt cgttcttctt tattatctgt gtctagtata  13680 

atgaggcgag tcgtgctagg cggagcggtg gtgtatccgg agggtcctcc tccttcgtac  13740 

gagagcgtga tgcagcagca gcaggcgacg gcggtgatgc aatccccact ggaggctccc  13800 

tttgtgcctc cgcgatacct ggcacctacg gagggcagaa acagcattcg ttactcggaa  13860 

ctggcacctc agtacgatac caccaggttg tatctggtgg acaacaagtc ggcggacatt  13920 

gcttctctga actatcagaa tgaccacagc aacttcttga ccacggtggt gcaaaacaat  13980 

gactttaccc ctacggaagc cagcacccag accattaact ttgatgaacg atcgcggtgg  14040 

ggcggtcagc taaaaaccat catgcatact aacatgccca acgtgaacga gtatatgttt  14100 

agtaacaagt tcaaagcgcg tgtgatggtg tccagaaaac ctcctgaggg tgttagagta  14160 

gacgataatt atgatcataa gcaagatatt ctaaaatacg agtggttcga gtttactttg  14220 

ccagaaggca acttttcggt cactatgact atcgacttga tgaacaatgc catcatagac  14280 

aattacttga aagtgggcag acagaatgga gtgttggaaa gtgacattgg tgttaagttc  14340 

gacactagga acttcaagtt gggatgggat ccagaaacta agttgatcat gcctggggtt  14400 

tacacctatg aggccttcca tcctgacatc gtattgctgc ctggctgcgg agtggacttt  14460 

accgaaagcc gtctgagcaa ccttcttggc attagaaaga aacacccatt ccaagagggt  14520 

tttaagatct tgtatgagga tttagaagga ggaaatattc cagccctttt ggatgtagat  14580 

gcttatgaga acagcaagaa agatcaaaaa gccaaaatag aagctgctgc agaagctaaa  14640 

gcaaacatag ttgccaacga tccggtaagg gtggctaacg ctagtgaaat caggggagac  14700 

agttttgccg caacatccgt tccgactaaa gaatcattat tggatgatgt gtctcaaaac  14760 

atagagttaa aactcactat taagcctgtg gaaaaagatg gcaaaaacag aagttacaat  14820 

gtgttggaag ataaaatcaa cacggcctat cgcagttggt acctttcgta caattatggc  14880 

gaccccgaaa aaggagtgcg ttcctggaca ttgctcacca cctcagatgt cacctgcgga  14940 

gcggagcagg tctactggtc gcttccagac atgatgcagg atcctgtcac tttccgctcc  15000 

actagacaag tcagtaacta ccctgtggtg ggtgcagagc ttatgcccgt cttttcaaag  15060 

agcttctaca acgaacaagc tgtgtactcc cagcagctcc gccagtccac ctcgcttacg  15120 

cacgtcttca accgctttcc tgagaaccag attttaatcc gtccgccggc gcccacaatt  15180 

accaccgtca gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gttgcgcagc  15240 

agtatccggg gagtccaacg tgtgaccgtt actgacgcca gacgccgcac ctgtccctac  15300 

gtgtacaagg cactgggcat agtcgcaccg cgcgtccttt caagccgcac tttctaaaaa  15360 

aaaaaaaaaa atgtccgttc ttatctcgcc cagtaataac accggttggg gtctgcgcgc  15420 

tcccagcaag atgtacggag gcgcacgcaa acgttctacc caacatcccg tgcgtgttcg  15480 

cgggcatttt cgcgctccat ggggtgccct caagggccgc actcgcgttc gaaccaccgt  15540 

cgatgatgta atcgatcagg tggttgccga cgcccgtaat tatactccta ctgcgcctac  15600 

atctactgtg gacgcagtta ttgacagtgt agtggctgac gctcgcaact atgctcgacg  15660 

taagagccgg cgaaggcgca ttgccagacg tcaccgagct accactgcca tgcgagcagc  15720 

aagagctctg ctacgaagag ctagacgcgt ggggcgaaga gccatgctta gggcggccag  15780 

acgtgcagct tcgggcgcca gcgccggcag gtcccgcagg caagcagccg ctgtcgcagc  15840 

ggcgactatt gccgacatgg cccaatcgcg aagaggcaat gtatactggg tgcgtgacgc  15900 

tgccaccggt caacgtgtac ccgtgcgcac ccgtccccct cgcacttaga agatactgag  15960 

cagtctccga tgttgtgtcc cagcggcgag gatgtccaag cgcaaataca aggaagaaat  16020 

gctgcaggtt atcgcacctg aagtctacgg ccaaccgttg aaggatgaaa aaaaaccccg  16080 

caaaatcaag cgggtaaaaa aggacaaaaa agaagaggaa gatggcgatg atgggctggc  16140 

ggagtttgtg cgcgagtttg ccccacggcg acgcgtgcaa tggcgtgggc gcaaagttcg  16200 

acatgtgttg agacctggaa cttcggtggt ctttacaccc ggcgagcgtt caagcgctac  16260 

ttttaagcgt tcctatgatg aggtgtacgg ggatgatgat attcttgagc aggcagctga  16320 

ccgattaggc gagtttgctt atggcaagcg tagtagaata aatcccaagg atgaaacagt  16380 

gtccataccc ttggatcatg gaaatcccac ccctagtctt aaaccggtca ctttgcagca  16440 

agtgttaccc gtaactccgc gaacaggtgt taaacgcgaa ggtgaagatt tgtatcccac  16500 

tatgcaactg atggtgccca aacgccagaa gttggaggac gttttggaga aagtaaaagt  16560 

ggatccagat attcaacctg aggttaaagt gagacccatt aagcaggtag cgcctggtct  16620 

gggagtacaa actgtagaca ttaaaattcc cactgaaagt atggaagtgc aaactgaacc  16680 

cgcaaagcct actgccacct ccactgaagt gcaaacggac ccatggatgc ccatgcctat  16740 

tacaactgac gccgtcggtc ccactcgaag atcccgacga aagtacggtc cagcaagtct  16800 

gttgatgccc aactatgtcg tacacccatc tattattcct actcctggtt accgaggcac  16860 

tcgctactat cgcagccgaa acagtacttc ccgccgtcgc cgcaagacac ctgcaaatcg  16920 

cagtcgtcgc cgtagacgca caagcaaacc gattcccggc gccctggtgc ggcaagtgta  16980 

ccgcaatggt agtgcggaac ctttgacact gccgcgtgcg cgttaccatc ctagtatcat  17040 

cacttaatca atgttgccgc tgcctccttg cagatatggc cctcacttgt cgccttcgcg  17100 

ttcccatcac tggttaccga ggaagaaact cgcgccgtag aagagggatg ttggggcgcg  17160 

gaatgcgacg ctacaggcga cggcgtgcta tccgcaagca attgcggggt ggttttttgc  17220 

cagccttaat tccaattatc gctgctgcga ttggcgcaat accaggcata gcttccgtgg  17280 

cggttcaggc ctcgcaacga cattgacatt ggaaaaaaaa aaaacgtata aataaaaaat  17340 

acaatggact ctgacactcc tggtactgtg actatgtttt cttagagatg gaagacatca  17400 

atttttcatc cttggctccg cgacacggca cgaagccgta catgggcacc tggagcgaca  17460 

tcggcacgag ccaactgaac gggggcgcct tcaattggag cagtatctgg agcgggctta  17520 

aaaattttgg ctcaaccata aaaacatacg ggaacaaagc ttggaacagc agtacaggac  17580 

aggcgcttag aaataaactt aaagaccaga acttccaaca aaaagtagtc gatgggatag  17640 

cttccggtat caatggagtg gtagatttgg ctaaccaggc tgtgcagaaa aagataaaca  17700 

gtcgtttgga cccgccgcca gcaaccccag gtgaaatgca agtggaggaa gaaattcctc  17760 

cgccagaaaa acgaggcgac aagcgtccgc gtcccgattt ggaagagacg ctggtgacgc  17820 

gcgtagatga accgccttct tatgaggaag caacgaagct tggaatgccc accactagac  17880 

cgatagcccc tatggccacc ggggtgatga aaccttctca gttgcatcga cccgtcacct  17940 

tggatttgcc ccctcctcct gctgctactg ctgtacccgc ttctaagcct gtcgctgccc  18000 

cgaaaccagt cgccgtagcc aggtcacgtc ccgggggcgc tcctcgtcca aatgcacact  18060 

ggcaaaatac tctgaacagc atcgtgggtc taggcgtgca aagtgtaaaa cgccgtcgct  18120 

gcttttaatt aaatatggag tagcgcttaa cttgcctatc tgtgtatatg tgtcattaca  18180 

cgccgtcaca gcatcagagg aaaaaaggaa gaggtcgtgc gtcgacgctg agttactttc  18240 

aagatggcca ccccatcgat gctgccccaa tgggcataca tgcacatcgc cggacaggat  18300 

gcttcggagt acctgagtcc gggtctggtg cagttcgccc gcgccacaga cacctacttc  18360 

aatctgggaa ataagtttag aaatcctacc gtagcgccga cccacgatgt gaccaccgat  18420 

cgtagccagc ggctcatgtt gcgcttcgtg cccgttgacc gggaggacaa tacatactct  18480 

tacaaagtgc ggtacaccct ggccgtgggc gacaacagag tgctggatat ggccagcacg  18540 

ttctttgaca ttaggggcgt gttggacaga ggtcccagtt ttaaacccta ttctggtacg  18600 

gcttacaact ccctggctcc taaaggcgct ccaaatgcat ctcagtggtt ggataaggga  18660 

gttacaagca ctggcctagt ggacgacggc aatactgatg atggggaaga agccaaaaaa  18720 

gcaacataca cttttggtaa tgctccagta aaagccgagg ctgaaatcac aaaagacgga  18780 

ttgccggtgg gcttggaagt ttcaactgaa ggtcctaaac caatctatgc tgataagctt  18840 

tatcagccag aacctcaagt gggagacgaa acttggactg acctagacgg aaaaaccgaa  18900 

gagtatggag ggagggttct taaacctgaa actaaaatga aaccctgcta cggatctttt  18960 

gctaaaccta ctaatattaa aggaggtcag gcaaaggtaa aaccaaaaga agacgatggc  19020 

actaacaaca tcgagtatga cattgacatg aacttctttg acttaagatc acaaagatca  19080 

gaactcaaac ctaaaattgt aatgtatgca gaaaatgtgg acctggaatg tccagatact  19140 

catgttgtgt acaaacctgg agtttcagat gctagttctg agaccaatct tggacaacag  19200 

tctatgccca acagacccaa ctacattggc ttcagagata acttcatcgg acttatgtac  19260 

tataacagta ctggcaacat gggggtactg gctggccaag cgtctcagtt gaatgcagtg  19320 

gttgacttgc aggacagaaa cacagaactg tcttaccaac tcttgcttga ctctctgggc  19380 

gacagaacca gatactttag catgtggaat caggctgtgg acagttatga tcctgatgta  19440 

cgtgttattg aaaatcatgg tgtggaagat gaacttccca actattgttt tccgttggat  19500 

ggtgtcggtc cgcgaacaga tagttacaag gagattaagc caaatggaga ccaatctact  19560 

tggacaaatg tagacccaac tggcagcagt gaacttgcta agggaaatcc atttgccatg  19620 

gaaattaacc ttcaagccaa tctatggcga agtttccttt attccaatgt ggctctatat  19680 

ctcccagact cgtacaaata caccccgtcc aatgtcactc ttccagaaaa caaaaacacc  19740 

tacgactaca tgaacgggcg ggtggtgccg ccatctctag tagacaccta tgtgaacatt  19800 

ggtgccaggt ggtctctgga tgccatggac aatgtcaacc cattcaacca ccaccgtaac  19860 

gctggcttgc gttaccgatc catgcttctg ggtaacggac gttatgtgcc tttccacata  19920 

caagtgcctc aaaaattctt cgctgttaaa aacctgctgc ttctcccagg ctcctacact  19980 

tatgagtgga actttaggaa ggatgtaaac atggttctac agagttccct cggtaacgac  20040 

ctacgggtag atggcgccag catcagtttt acgagcatca acctctatgc tacttttttc  20100 

cccatggctc acaacaccgc ttccaccctt gaagccatgc tgcggaatga caccaatgat  20160 

cagtcattca acgactacct atctgcagct aacatgctct accccattcc tgccaatgca  20220 

accaatattc ccatttccat tccttctcgc aactgggcgg ctttcagagg ctggtcattt  20280 

accagactga aaaccaaaga aactccctct ttggggtctg gatttgaccc ctacttcgtc  20340 

tattctggtt ctattcccta cctggatggt accttctacc tgaaccacac ttttaagaag  20400 

gtttccatca tgtttgactc ttcagtgagc tggcctggaa atgacaggtt actatctcct  20460 

aacgaatttg aaataaagcg cactgtggat ggcgaaggct acaacgtagc ccaatgcaac  20520 

atgaccaaag actggttctt ggtacagatg ctcgccaact acaacatcgg ctatcagggc  20580 

ttctacattc cagaaggata caaagatcgc atgtattcat ttttcagaaa cttccagccc  20640 

atgagcaggc aggtggttga tgaggtcaat tacaaagact tcaaggccgt cgccataccc  20700 

taccaacaca acaactctgg ctttgtgggt tacatggctc cgaccatgcg tcaaggtcaa  20760 

ccctatcccg ctaactatcc ctatccactc attggaacaa ctgccgtaaa tagtgttacg  20820 

cagaaaaagt tcttgtgtga cagaaccatg tggcgcatac cgttctcaag caacttcatg  20880 

tctatgggag cccttacaga cttgggacag aacatgctct atgccaactc agctcatgct  20940 

ctggacatga cctttgaggt ggatcccatg gatgagccca ccctgcttta tcttctcttc  21000 

gaagttttcg acgtggtcag agtgcatcag ccacaccgcg gcatcatcga ggcagtctac  21060 

ctgcgtacac cgttctcggc cggtaacgct accacgtaag aagcttcttg cttcttgcaa  21120 

acagcagctg caaccatggc ctgcggatcc caaaacggct ccagcgagca agagctcaga  21180 

gccattgtcc aagacctggg ttgcggacca tattttttgg gaacctttga taagcgcttc  21240 

ccggggttca tggcccccga taagctcgcc tgtgccattg taaatacggc cggacgtgag  21300 

acggggggag agcactggtt ggctttcggt tggaacccac gttctaacac ctgctacctt  21360 

tttgatcctt ttggattctc ggatgatcgt ctcaaacaga tttaccagtt tgaatatgag  21420 

ggtctcctgc gccgcagcgc tcttgctacc aaggaccggt gtattacgct ggaaaaatct  21480 

acccagaccg tgcagggccc ccgttctgcc gcctgcggac ttttctgctg catgttcctt  21540 

catgcctttg tgcactggcc tgaccgtccc atggacggaa accccaccat gaaattgcta  21600 

actggagtgc caaacaacat gcttcattct cctaaagtcc agcccaccct gtgtgacaat  21660 

caaaaagcac tctaccattt tctcaatacc cattcgcctt attttcgctc tcatcgtaca  21720 

cacatcgaaa gggccactgc gttcgaccgt atggatgtgc aataatgatt catgtaaaca  21780 

acgtgttcaa taaacagcac tttatttttt acatgtatcg aggctctgga ttacttattt  21840 

atttacaagt cgaatgggtt ctgacgagaa tcagaatgac ccgcaggcag tgatacgttg  21900 

cggaactgat acttgggttg ccacttgaat tcgggaatca ccaacttggg aaccggtata  21960 

tcgggcagga tgtcactcca cagctttctg gtcagctgca aagctcccag caggtcagga  22020 

gccgaaatct tgaaatcaca attaggacca gtgctctgag cgcgagagtt gcggtacacc  22080 

ggattgcagc actgaaacac catcagcgac ggatgtctta cgcttgccag cacggtggga  22140 

tctgcaatca tgcccacatc cagatcttca gcattggcaa tgctgaacgg ggtcatcttg  22200 

caggtctgcc tacccatggc gggcacccaa ttaggcttgt ggttacaatc gcagtgcagg  22260 

gggatcagta tcatcttggc ctgatcctgt ctgattcctg gatacacggc tctcatgaaa  22320 

gcatcatatt gcttgaaagc ctgctgggct ttactaccct cggtataaaa catcccgcag  22380 

gacctgctcg aaaactggtt agctgcgcag ccggcatcat tcacacagca gcgggcgtca  22440 

ttgttggcta tttgcaccac acttctgccc cagcggtttt gggtgatttt ggttcgctcg  22500 

ggattctcct tcaaggctcg ttgtccgttc tcgctggcca catccatctc gataatctgc  22560 

tccttctgaa tcataatatt gccatgcaag cacttcagct tgccctcata atcattgcag  22620 

ccatgaggcc acaacgcaca gcctgtacat tcccaattat ggtgggcgat ctgagaaaaa  22680 

gaatgtatca ttccctgcag aaatcttccc atcatcgtgc tcagtgtctt gtgactagtg  22740 

aaagttaact ggatgcctcg gtgctcctcg ttcacgtact ggtgacagat gcgcttgtat  22800 

tgttcgtgct gctcaggcat tagtttaaaa gaggttctaa gttcgttatc cagcctgtac  22860 

ttctccatca gcagacacat cacttccatg cctttctccc aagcagacac caggggcaag  22920 

ctaatcggat tcttaacagt gcaggcagca gctcctttag ccagagggtc atctttggcg  22980 

atcttctcaa tgcttctttt gccatccttc tcaacgatgc gcacgggcgg gtagctgaaa  23040 

cccactgcta caagttgcgc ctcttctctt tcttcttcgc tgtcttgact gatgtcttgc  23100 

atggggacat gtttggtctt ccttggcttc tttttcgggg gtatcggagg aggaggactg  23160 

tcgctccgtt ccggagacag ggaggattgt gacgtttcgc tcaccattac caactgactg  23220 

tcggtagaag aacctgaccc cacacggcga caggtgtttc tcttcggggg cagaggtgga  23280 

ggcgattgcg aagggctgcg gtccgacctg gaaggcggat gactggcaga accccttccg  23340 

cgttcggggg tgtgctccct gtggcggtcg cttaactgat ttccttcgcg gctggccatt  23400 

gtgttctcct aggcagagaa acaacagaca tggaaactca gccattgctg tcaacatcgc  23460 

cacgagtgcc atcacatctc gtcctcagcg acgaggaaaa ggagcagagc ttaagcattc  23520 

caccgcccag tcctgccacc acctctaccc tagaagataa ggaggtcgac gcatctcatg  23580 

acatgcagaa taaaaaagcg aaagagtctg agccagacat cgaacaagac ccgggctatg  23640 

tgacaccggt ggaacacgag gaagagttga aacgctttct agagagagag gatgaaaact  23700 

gcccaaaaca gcaagcggat aactatcacc aagatgctgg aaatagggat cagaacaccg  23760 

actacctcat agggcttgac ggggaagacg cgctccttaa acatctagca agacagtcac  23820 

tcatagtcaa ggatgcatta ttggacagaa ctgaagtgcc catcagtgtc gaagagctca  23880 

gccgcgccta cgagcttaac ctattttcac ctcgtactcc ccccaaacgt cagccaaacg  23940 

gcacctgcga gccaaatcct cgcttaaact tttatccagc ttttgctgtg ccagaagtac  24000 

tggctaccta tcacatcttt tttaaaaatc aaaaaattcc agtctcctgc cgcgctaatc  24060 

gcacccgcgc cgatgcccta ctcaatctgg gacctggttc acgcttacct gatatagctt  24120 

ccttggaaga ggttccaaag atcttcgagg gtctgggcaa taatgagact cgggccgcaa  24180 

atgctctgca aaagggagaa aatggcatgg atgagcatca cagcgttctg gtggaattgg  24240 

aaggcgataa tgccagactc gcagtactca agcgaagcgt cgaggtcaca cactttgcat  24300 

accccgctgt caacctgccc cctaaagtca tgacggccgt catggaccag ttactcatta  24360 

agcgcgcaag tcccctttca gaagacatgc atgacccaga tgcctgtgat gagggtaaac  24420 

cagtggtcag tgatgagcag ctaacccgat ggctgggcac cgactctccc cgggatttgg  24480 

aagagcgtcg caagcttatg atggccgtgg tgctggttac cgtagaacta gagtgtcttc  24540 

ggcgtttctt taccgattca gaaaccttgc gcaaactcga agagaatctg cactacactt  24600 

ttagacacgg ctttgtgcgg caggcatgca agatatctaa cgtggaactc accaacctgg  24660 

tttcctacat gggtattctg catgagaatc gcctaggaca aagcgtgctg cacagcaccc  24720 

ttaaggggga agcccgccgt gattacatcc gcgattgtgt ttatctctac ctgtgccaca  24780 

cgtggcaaac cggcatgggt gtatggcagc aatgtttaga agaacagaac ctgaaagagc  24840 

taaacaagct cttacagaaa tctcttaagg ttctgtggac agggttcgac gagcgcaccg  24900 

tcgcttccga cctggcagac ctcatcttcc cagagcgtct cagggttact ttgcgaaacg  24960 

gactgcctga ctttatgagc cagagcatgc ttaacaattt tcgctctttc atcctggaac  25020 

gctccggtat cctgcccgcc acctgctgcg cactgccctc cgactttgtg cctctcacct  25080 

accgcgaatg ccccccgccg ctatggagtc actgctacct gttccgtctg gccaactacc  25140 

tctcctacca ctcggatgtg atcgaggatg tgagcggaga cggcttgctg gagtgtcact  25200 

gccgctgcaa tctgtgcacg ccccaccggt ccctagcttg caacccccag ttgatgagcg  25260 

aaacccagat aataggcacc tttgaattgc aaggccccag cagccaaggc gatgggtctt  25320 

ctcctgggca aagtttaaaa ctgaccccgg gactgtggac ctccgcctac ttgcgcaagt  25380 

ttgccccgga agattaccac ccctatgaaa tcaagttcta tgaggaccaa tcacagcctc  25440 

cgaaagccga actttcggcc tgcgtcatca cccagggggc aattctggcc caattgcaag  25500 

ccatccaaaa atcccgccaa gaatttctac tgaaaaaggg taagggggtc taccttgacc  25560 

cccagaccgg cgaggaactc aacacaaggt tccctcagga tgtcccaacg acgagaaagc  25620 

aagaagttga aggtgcagcc gccgccccca gaagatatgg aggaagattg ggacagtcag  25680 

gcagaggaag cggaggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag  25740 

gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg  25800 

gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt  25860 

cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc  25920 

ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc  25980 

tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat  26040 

ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat  26100 

agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa  26160 

accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac  26220 

agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc  26280 

catcttccag cagagtcggg gccaagagca ggaactgaaa ataaaaaacc gatctctgcg  26340 

ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga  26400 

ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac  26460 

cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca  26520 

cgccttacat gtggagttat cagccccaaa tgggattggc ggcaggcgcc tcccaggact  26580 

actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata  26640 

tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc  26700 

aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca  26760 

ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc  26820 

agttagcggg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga  26880 

tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac  26940 

gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg  27000 

ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc  27060 

aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc  27120 

actacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg  27180 

attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg  27240 

ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca  27300 

ccctcaaggt ccggcccacg gagtgcggat tactatcgaa ggcaaaatac actctcgcct  27360 

gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt  27420 

ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg  27480 

tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg  27540 

gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct  27600 

ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta  27660 

ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg  27720 

tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct  27780 

acctatacac accttgcttc actttcctag tggtgttgtg gtattggttt aaaaaatggg  27840 

gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga  27900 

tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg  27960 

catctgtgga gttcttatta agtgcggatg ggaatgcagg tccgttgaaa ttacacacaa  28020 

taacaaaacc tggaacaata ccttatccac cacatgggag ccaggagttc ccgagtggta  28080 

cactgtctct gtccgaggtc ctgacggttc catccgcatt agtaacaaca ctttcatttt  28140 

ttctgaaatg tgcgatctgg ccatgttcat gagcaaacag tattctctat ggcctcctag  28200 

caaggacaac atcgtaacgt tctccattgc ttattgcttg tgcgcttgcc ttcttactgc  28260 

tttactgtgc gtatgcatac acctgcttgt aaccactcgc atcaaaaacg ccaataacaa  28320 

agaaaaaatg ccttaacctc tttctgttta cagacatggc ttctcttaca tctctcatat  28380 

ttgtcagcat tgtcactgcc gctcacggac aaacagtcgt ctctatccct ctaggacata  28440 

attacactct cataggaccc ccaatcactt cagaggtcat ctggaccaaa ctgggaagcg  28500 

ttgattactt tgatataatc tgcaacaaaa caaaaccaat aatagtaact tgcaacatac  28560 

aaaatcttac attgattaat gttagcaaag tttacagcgg ttactattat ggttatgaca  28620 

gatacagtag tcaatataga aattacttgg ttcgtgttac ccagttaaaa accacgaaaa  28680 

tgccaaatat ggcaaagatt cgatccgatg acaattctct agaaactttt acatctccca  28740 

ccacacccga cgaaaaaaac atcccagatt caatgattgc aattgttgca gcggtggcag  28800 

tggtgatggc actaataata atatgcatgc ttttatatgc ttgtcgctac aaaaagtttc  28860 

atcctaaaaa acaagatctc ctactaaggc ttaacattta atttcttttt atacagccat  28920 

ggtttccact accacattcc ttatgcttac tagtcttgca actctgactt ctgctcgctc  28980 

acacctcact gtaactatag gctcaaactg cacactaaaa ggacctcaag gtggtcatgt  29040 

cttttggtgg agaatatatg acaatggatg gtttacaaaa ccatgtgacc aacctggtag  29100 

atttttctgc aacggcagag acctaaccat tatcaacgtg acagcaaatg acaaaggctt  29160 

ctattatgga accgactata aaagtagttt agattataac attattgtac tgccatctac  29220 

cactccagca ccccgcacaa ctactttctc tagcagcagt gtcgctaaca atacaatttc  29280 

caatccaacc tttgccgcgc ttttaaaacg cactgtgaat aattctacaa cttcacatac  29340 

aacaatttcc acttcaacaa tcagcattat cgctgcagtg acaattggaa tatctattct  29400 

tgtttttacc ataacctact acgcctgctg ctatagaaaa gacaaacata aaggtgatcc  29460 

attacttaga tttgatattt aatttgttct tttttttttt atttacagta tggtgaacac  29520 

caatcatggt acctagaaat ttcttcttca ccatactcat ttgtgcattt aatgtttgcg  29580 

ctactttcac agcagtagcc acagcaaccc cagactgtat aggagcattt gcttcctatg  29640 

cactttttgc ttttgttact tgcatctgcg tatgtagcat agtctgcctg gttattaatt  29700 

ttttccaact tctagactgg atccttgtgc gaattgccta cctgcgccac catcccgaat  29760 

accgcaacca aaatatcgcg gcacttctta gactcatcta aaaccatgca ggctatacta  29820 

ccaatatttt tgcttctatt gcttccctac gctgtctcaa ccccagctgc ctatagtact  29880 

ccaccagaac accttagaaa atgcaaattc caacaaccgt ggtcatttct tgcttgctat  29940 

cgagaaaaat cagaaattcc cccaaattta ataatgattg ctggaataat taatataatc  30000 

tgttgcacca taatttcatt tttgatatac cccctatttg attttggctg gaatgctccc  30060 

aatgcacatg atcatccaca agacccagag gaacacattc ccctacaaaa catgcaacat  30120 

ccaatagcgc taatagatta cgaaagtgaa ccacaacccc cactactccc tgctattagt  30180 

tacttcaacc taaccggcgg agatgactga aacactcacc acctccaatt ccgccgagga  30240 

tctgctcgat atggacggcc gcgtctcaga acagcgactt gcccaactac gcatccgcca  30300 

gcagcaggaa cgcgcggcca aagagctcag agatgtcatc caaattcacc aatgcaaaaa  30360 

aggcatattc tgtttggtaa aacaagccaa gatatcctac gagatcaccg ctactgacca  30420 

tcgcctctct tacgaacttg gcccccaacg acaaaaattt acctgcatgg tgggaatcaa  30480 

ccccatagtt atcacccagc aaagtggaga tactaagggt tgcattcact gctcctgcga  30540 

ttccatcgag tgcacctaca ccctgctgaa gaccctatgc ggcctaagag acctgctacc  30600 

aatgaattaa aaaatgatta ataaaaaatc acttacttga aatcagcaat aaggtctctg  30660 

ttgaaatttt ctcccagcag cacctcactt ccctcttccc aactctggta ttctaaaccc  30720 

cgttcagcgg catactttct ccatacttta aaggggatgt caaattttag ctcctctcct  30780 

gtacccacaa tcttcatgtc tttcttccca gatgaccaag agagtccggc tcagtgactc  30840 

cttcaaccct gtctacccct atgaagatga aagcacctcc caacacccct ttataaaccc  30900 

agggtttatt tccccaaatg gcttcacaca aagcccagac ggagttctta ctttaaaatg  30960 

tttaacccca ctaacaacca caggcggatc tctacagcta aaagtgggag ggggacttac  31020 

agtggatgac actgatggta ccttacaaga aaacatacgt gctacagcac ccattactaa  31080 

aaataatcac tctgtagaac tatccattgg aaatggatta gaaactcaaa acaataaact  31140 

atgtgccaaa ttgggaaatg ggttaaaatt taacaacggt gacatttgta taaaggatag  31200 

tattaacacc ttatggactg gaataaaccc tccacctaac tgtcaaattg tggaaaacac  31260 

taatacaaat gatggcaaac ttactttagt attagtaaaa aacggagggc ttgttaatgg  31320 

ctacgtgtct ctagttggtg tatcagacac tgtgaaccaa atgttcacac aaaagacagc  31380 

aaacatccaa ttaagattat attttgactc ttctggaaat ctattaactg atgaatcaga  31440 

cttaaaaatt ccacttaaaa ataaatcttc tacagcgacc agtgaaactg tagccagcag  31500 

caaagccttt atgccaagta ctacagctta tcccttcaac accactacta gggatagtga  31560 

aaactacatt catggaatat gttactacat gactagttat gatagaagtc tatttccctt  31620 

gaacatttct ataatgctaa acagccgtat gatttcttcc aatgttgcct atgccataca  31680 

atttgaatgg aatctaaatg caagtgaatc tccagaaagc aacatagcta cgctgaccac  31740 

atcccccttt ttcttttctt acattacaga agacgacaac taaaataaag tttaagtgtt  31800 

tttatttaaa atcacaaaat tcgagtagtt attttgcctc caccttccca tttgacagaa  31860 

tacaccaatc tctccccacg cacagcttta aacatttgga taccattaga gatagacatt  31920 

gttttagatt ccacattcca aacagtttca gagcgagcca atctggggtc agtgatagat  31980 

aaaaatccat cgcgatagtc ttttaaagcg ctttcacagt ccaactgctg cggatgcgaa  32040 

tccggagtct ggatcacggt catctggaag aagaacgatg ggaatcataa tccgaaaacg  32100 

gtatcggacg attgtgtctc atcaaaccca caagcagccg ctgtctgcgt cgctccgtgc  32160 

aactgctgtt tatgggatca gggtccacag tgtcctgaag catgatttta atagccctta  32220 

acatcaactt tctggtgcga tgcgcgcagc aacgcattct gatttcactc aaatctttgc  32280 

agtaggtaca acacattatt acaatattgt ttaataaacc ataattaaaa gcgctccagc  32340 

caaaactcat atctgatata atcgcccctg catgaccatc ataccaaagt ttaatataaa  32400 

ttaaatgacg ttccctcaaa aacacactac ccacatacat gatctctttt ggcatgtgca  32460 

tattaacaat ctgtctgtac catggacaac gttggttaat catgcaaccc aatataacct  32520 

tccggaacca cactgccaac accgctcccc cagccatgca ttgaagtgaa ccctgctgat  32580 

tacaatgaca atgaagaacc caattctctc gaccgtgaat cacttgagaa tgaaaaatat  32640 

ctatagtggc acaacataga cataaatgca tgcatcttct cataattttt aactcctcag  32700 

gatttagaaa catatcccag ggaataggaa gctcttgcag aacagtaaag ctggcagaac  32760 

aaggaagacc acgaacacaa cttacactat gcatagtcat agtatcacaa tctggcaaca  32820 

gcgggtggtc ttcagtcata gaagctcggg tttcattttc ctcacaacgt ggtaactggg  32880 

ctctggtgta agggtgatgt ctggcgcatg atgtcgagcg tgcgcgcaac cttgtcataa  32940 

tggagttgct tcctgacatt ctcgtatttt gtatagcaaa acgcggccct ggcagaacac  33000 

actcttcttc gccttctatc ctgccgctta gcgtgttccg tgtgatagtt caagtacagc  33060 

cacactctta agttggtcaa aagaatgctg gcttcagttg taatcaaaac tccatcgcat  33120 

ctaattgttc tgaggaaatc atccacggta gcatatgcaa atcccaacca agcaatgcaa  33180 

ctggattgcg tttcaagcag gagaggagag ggaagagacg gaagaaccat gttaattttt  33240 

attccaaacg atctcgcagt acttcaaatt gtagatcgcg cagatggcat ctctcgcccc  33300 

cactgtgttg gtgaaaaagc acagctaaat caaaagaaat gcgattttca aggtgctcaa  33360 

cggtggcttc caacaaagcc tccacgcgca catccaagaa caaaagaata ccaaaagaag  33420 

gagcattttc taactcctca atcatcatat tacattcctg caccattccc agataatttt  33480 

cagctttcca gccttgaatt attcgtgtca gttcttgtgg taaatccaat ccacacatta  33540 

caaacaggtc ccggagggcg ccctccacca ccattcttaa acacaccctc ataatgacaa  33600 

aatatcttgc tcctgtgtca cctgtagcga attgagaatg gcaacatcaa ttgacatgcc  33660 

cttggctcta agttcttctt taagttctag ttgtaaaaac tctctcatat tatcaccaaa  33720 

ctgcttagcc agaagccccc cgggaacaag agcaggggac gctacagtgc agtacaagcg  33780 

cagacctccc caattggctc cagcaaaaac aagattggaa taagcatatt gggaaccgcc  33840 

agtaatatca tcgaagttgc tggaaatata atcaggcaga gtttcttgta aaaattgaat  33900 

aaaagaaaaa tttgccaaaa aaacattcaa aacctctggg atgcaaatgc aataggttac  33960 

cgcgctgcgc tccaacattg ttagttttga attagtctgc aaaaataaaa aaaaaaacaa  34020 

gcgtcatatc atagtagcct gacgaacagg tggataaatc agtctttcca tcacaagaca  34080 

agccacaggg tctccagctc gaccctcgta aaacctgtca tggtgattaa acaacagcac  34140 

cgaaagttcc tcgcggtgac cagcatgaat aattcttgat gaagcataca atccagacat  34200 

gttagcatca gttaacgaga aaaaacagcc aacatagcct ttgggtataa ttatgcttaa  34260 

tcgtaagtat agcaaagcca cccctcgcgg atacaaagta aaaggcacag gagaataaaa  34320 

aatataatta tttctctgct gctgttcagg caacgtcgcc cccggtccct ctaaatacac  34380 

atacaaagcc tcatcagcca tggcttacca gacaaagtac agcgggcacg cacaagctct  34440 

aaagtcactc tccaacctct ccacaatata tatacacaag ccctaaactg acgtaatggg  34500 

agtaaagtgt aaaaaatccc gccaaaccca acacacaccc cgaaactgcg tcaccaggga  34560 

aaagtacagt ttcacttccg caatcccaac aagcgtcact tcctctttct cacggtacgt  34620 

cacatcccat taacttgcaa cgtcattttc ccacggccgc gccgccccgt ttagccgtta  34680 

accccacagc caatcaccac acaccccaca atttttaaaa tcacctcatt tacatattgg  34740 

caccattcca tctataaggt atattattga tgatg                             34775 

 
           
             2  
             49  
             DNA  
             Artificial Sequence  
             
               synthetic polyA signal  
             
           
            2 

aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtg                 49 

 
           
             3  
             1521  
             DNA  
             Artificial Sequence  
             
               optimized human HIV-1 gag open reading frame  
             
           
            3 

atgggtgcta gggcttctgt gctgtctggt ggtgagctgg acaagtggga gaagatcagg     60 

ctgaggcctg gtggcaagaa gaagtacaag ctaaagcaca ttgtgtgggc ctccagggag    120 

ctggagaggt ttgctgtgaa ccctggcctg ctggagacct ctgaggggtg caggcagatc    180 

ctgggccagc tccagccctc cctgcaaaca ggctctgagg agctgaggtc cctgtacaac    240 

acagtggcta ccctgtactg tgtgcaccag aagattgatg tgaaggacac caaggaggcc    300 

ctggagaaga ttgaggagga gcagaacaag tccaagaaga aggcccagca ggctgctgct    360 

ggcacaggca actccagcca ggtgtcccag aactacccca ttgtgcagaa cctccagggc    420 

cagatggtgc accaggccat ctccccccgg accctgaatg cctgggtgaa ggtggtggag    480 

gagaaggcct tctcccctga ggtgatcccc atgttctctg ccctgtctga gggtgccacc    540 

ccccaggacc tgaacaccat gctgaacaca gtggggggcc atcaggctgc catgcagatg    600 

ctgaaggaga ccatcaatga ggaggctgct gagtgggaca ggctgcatcc tgtgcacgct    660 

ggccccattg cccccggcca gatgagggag cccaggggct ctgacattgc tggcaccacc    720 

tccaccctcc aggagcagat tggctggatg accaacaacc cccccatccc tgtgggggaa    780 

atctacaaga ggtggatcat cctgggcctg aacaagattg tgaggatgta ctcccccacc    840 

tccatcctgg acatcaggca gggccccaag gagcccttca gggactatgt ggacaggttc    900 

tacaagaccc tgagggctga gcaggcctcc caggaggtga agaactggat gacagagacc    960 

ctgctggtgc agaatgccaa ccctgactgc aagaccatcc tgaaggccct gggccctgct   1020 

gccaccctgg aggagatgat gacagcctgc cagggggtgg ggggccctgg tcacaaggcc   1080 

agggtgctgg ctgaggccat gtcccaggtg accaactccg ccaccatcat gatgcagagg   1140 

ggcaacttca ggaaccagag gaagacagtg aagtgcttca actgtggcaa ggtgggccac   1200 

attgccaaga actgtagggc ccccaggaag aagggctgct ggaagtgtgg caaggagggc   1260 

caccagatga aggactgcaa tgagaggcag gccaacttcc tgggcaaaat ctggccctcc   1320 

cacaagggca ggcctggcaa cttcctccag tccaggcctg agcccacagc ccctcccgag   1380 

gagtccttca ggtttgggga ggagaagacc acccccagcc agaagcagga gcccattgac   1440 

aaggagctgt accccctggc ctccctgagg tccctgtttg gcaacgaccc ctcctcccag   1500 

taaaataaag cccgggcaga t                                             1521 

 
           
             4  
             2550  
             DNA  
             Artificial Sequence  
             
               gag expression cassette  
             
           
            4 

ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca     60 

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca    120 

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct    180 

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta    240 

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac    300 

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt    360 

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag    420 

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat    480 

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat    540 

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc    600 

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt    660 

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga    720 

caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt    780 

gccaagagtg agatctacca tgggtgctag ggcttctgtg ctgtctggtg gtgagctgga    840 

caagtgggag aagatcaggc tgaggcctgg tggcaagaag aagtacaagc taaagcacat    900 

tgtgtgggcc tccagggagc tggagaggtt tgctgtgaac cctggcctgc tggagacctc    960 

tgaggggtgc aggcagatcc tgggccagct ccagccctcc ctgcaaacag gctctgagga   1020 

gctgaggtcc ctgtacaaca cagtggctac cctgtactgt gtgcaccaga agattgatgt   1080 

gaaggacacc aaggaggccc tggagaagat tgaggaggag cagaacaagt ccaagaagaa   1140 

ggcccagcag gctgctgctg gcacaggcaa ctccagccag gtgtcccaga actaccccat   1200 

tgtgcagaac ctccagggcc agatggtgca ccaggccatc tccccccgga ccctgaatgc   1260 

ctgggtgaag gtggtggagg agaaggcctt ctcccctgag gtgatcccca tgttctctgc   1320 

cctgtctgag ggtgccaccc cccaggacct gaacaccatg ctgaacacag tggggggcca   1380 

tcaggctgcc atgcagatgc tgaaggagac catcaatgag gaggctgctg agtgggacag   1440 

gctgcatcct gtgcacgctg gccccattgc ccccggccag atgagggagc ccaggggctc   1500 

tgacattgct ggcaccacct ccaccctcca ggagcagatt ggctggatga ccaacaaccc   1560 

ccccatccct gtgggggaaa tctacaagag gtggatcatc ctgggcctga acaagattgt   1620 

gaggatgtac tcccccacct ccatcctgga catcaggcag ggccccaagg agcccttcag   1680 

ggactatgtg gacaggttct acaagaccct gagggctgag caggcctccc aggaggtgaa   1740 

gaactggatg acagagaccc tgctggtgca gaatgccaac cctgactgca agaccatcct   1800 

gaaggccctg ggccctgctg ccaccctgga ggagatgatg acagcctgcc agggggtggg   1860 

gggccctggt cacaaggcca gggtgctggc tgaggccatg tcccaggtga ccaactccgc   1920 

caccatcatg atgcagaggg gcaacttcag gaaccagagg aagacagtga agtgcttcaa   1980 

ctgtggcaag gtgggccaca ttgccaagaa ctgtagggcc cccaggaaga agggctgctg   2040 

gaagtgtggc aaggagggcc accagatgaa ggactgcaat gagaggcagg ccaacttcct   2100 

gggcaaaatc tggccctccc acaagggcag gcctggcaac ttcctccagt ccaggcctga   2160 

gcccacagcc cctcccgagg agtccttcag gtttggggag gagaagacca cccccagcca   2220 

gaagcaggag cccattgaca aggagctgta ccccctggcc tccctgaggt ccctgtttgg   2280 

caacgacccc tcctcccagt aaaataaagc ccgggcagat ctgatctgct gtgccttcta   2340 

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca   2400 

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc   2460 

attctattct ggggggtggg gtggggcagc acagcaaggg ggaggattgg gaagacaata   2520 

gcaggcatgc tggggatgcg gtgggctcta                                    2550 

 
           
             5  
             2662  
             DNA  
             Artificial Sequence  
             
               SEAP expression cassette  
             
           
            5 

ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca     60 

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca    120 

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct    180 

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta    240 

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac    300 

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt    360 

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag    420 

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat    480 

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat    540 

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc    600 

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt    660 

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga    720 

caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt    780 

gccaagagtg agatctaagt aagcttcctg catgctgctg ctgctgctgc tgctgggcct    840 

gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa    900 

ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc    960 

caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag   1020 

gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg   1080 

cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg   1140 

agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag   1200 

tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat   1260 

gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca   1320 

cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga   1380 

cgtgcctgcc tccgcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa   1440 

catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc   1500 

agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct   1560 

ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct   1620 

catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga   1680 

catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga   1740 

ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg   1800 

tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat   1860 

gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct   1920 

cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc   1980 

catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata   2040 

cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga   2100 

gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc   2160 

aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca   2220 

ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc   2280 

ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt aacccgtggt   2340 

ccccgcgttg cttcctctgc tggccgggac atcaggtggc ccccgctgaa ttggaatcga   2400 

tcagaattca gtcgacgata tctgatcacg atctgatctg ctgtgccttc tagttgccag   2460 

ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact   2520 

gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt   2580 

ctggggggtg gggtggggca gcacagcaag ggggaggatt gggaagacaa tagcaggcat   2640 

gctggggatg cggtgggctc ta                                            2662 

 
           
             6  
             37474  
             DNA  
             Artificial Sequence  
             
               pMRKAd5HIV-1 gag, coding  
             
           
            6 

ttcttaatta acatcatcaa taatatacct tattttggat tgaagccaat atgataatga     60 

gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt    120 

ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag    180 

tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc    240 

ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact    300 

gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta atatttgtct    360 

agggccgcgg ggactttgac cgtttacgtg gagactcgcc caggtgtttt tctcaggtgt    420 

tttccgcgtt ccgggtcaaa gttggcgttt tattattata ggcggccgcg atccattgca    480 

tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc    540 

atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca    600 

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc    660 

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat    720 

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt    780 

acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc    840 

cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta    900 

cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg    960 

atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt   1020 

gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac   1080 

gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa   1140 

ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga   1200 

ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag   1260 

tgagatctac catgggtgct agggcttctg tgctgtctgg tggtgagctg gacaagtggg   1320 

agaagatcag gctgaggcct ggtggcaaga agaagtacaa gctaaagcac attgtgtggg   1380 

cctccaggga gctggagagg tttgctgtga accctggcct gctggagacc tctgaggggt   1440 

gcaggcagat cctgggccag ctccagccct ccctgcaaac aggctctgag gagctgaggt   1500 

ccctgtacaa cacagtggct accctgtact gtgtgcacca gaagattgat gtgaaggaca   1560 

ccaaggaggc cctggagaag attgaggagg agcagaacaa gtccaagaag aaggcccagc   1620 

aggctgctgc tggcacaggc aactccagcc aggtgtccca gaactacccc attgtgcaga   1680 

acctccaggg ccagatggtg caccaggcca tctccccccg gaccctgaat gcctgggtga   1740 

aggtggtgga ggagaaggcc ttctcccctg aggtgatccc catgttctct gccctgtctg   1800 

agggtgccac cccccaggac ctgaacacca tgctgaacac agtggggggc catcaggctg   1860 

ccatgcagat gctgaaggag accatcaatg aggaggctgc tgagtgggac aggctgcatc   1920 

ctgtgcacgc tggccccatt gcccccggcc agatgaggga gcccaggggc tctgacattg   1980 

ctggcaccac ctccaccctc caggagcaga ttggctggat gaccaacaac ccccccatcc   2040 

ctgtggggga aatctacaag aggtggatca tcctgggcct gaacaagatt gtgaggatgt   2100 

actcccccac ctccatcctg gacatcaggc agggccccaa ggagcccttc agggactatg   2160 

tggacaggtt ctacaagacc ctgagggctg agcaggcctc ccaggaggtg aagaactgga   2220 

tgacagagac cctgctggtg cagaatgcca accctgactg caagaccatc ctgaaggccc   2280 

tgggccctgc tgccaccctg gaggagatga tgacagcctg ccagggggtg gggggccctg   2340 

gtcacaaggc cagggtgctg gctgaggcca tgtcccaggt gaccaactcc gccaccatca   2400 

tgatgcagag gggcaacttc aggaaccaga ggaagacagt gaagtgcttc aactgtggca   2460 

aggtgggcca cattgccaag aactgtaggg cccccaggaa gaagggctgc tggaagtgtg   2520 

gcaaggaggg ccaccagatg aaggactgca atgagaggca ggccaacttc ctgggcaaaa   2580 

tctggccctc ccacaagggc aggcctggca acttcctcca gtccaggcct gagcccacag   2640 

cccctcccga ggagtccttc aggtttgggg aggagaagac cacccccagc cagaagcagg   2700 

agcccattga caaggagctg taccccctgg cctccctgag gtccctgttt ggcaacgacc   2760 

cctcctccca gtaaaataaa gcccgggcag atctgctgtg ccttctagtt gccagccatc   2820 

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct   2880 

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg   2940 

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg   3000 

ggatgcggtg ggctctatgg ccgatcggcg cgccgtactg aaatgtgtgg gcgtggctta   3060 

agggtgggaa agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca   3120 

gccgccgccg ccatgagcac caactcgttt gatggaagca ttgtgagctc atatttgaca   3180 

acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga tgggctccag cattgatggt   3240 

cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc tggaacgccg   3300 

ttggagactg cagcctccgc cgccgcttca gccgctgcag ccaccgcccg cgggattgtg   3360 

actgactttg ctttcctgag cccgcttgca aacagtgcag cttcccgttc atccgcccgc   3420 

gatgacaagt tgacggctct tttggcacaa ttggattctt tgacccggga acttaatgtc   3480 

gtttctcagc agctgttgga tctgcgccag caggtttctg ccctgaaggc ttcctcccct   3540 

cccaatgcgg tttaaaacat aaataaaaaa ccagactctg tttggatttg gatcaagcaa   3600 

gtgtcttgct gtctttattt aggggttttg cgcgcgcggt aggcccggga ccagcggtct   3660 

cggtcgttga gggtcctgtg tattttttcc aggacgtggt aaaggtgact ctggatgttc   3720 

agatacatgg gcataagccc gtctctgggg tggaggtagc accactgcag agcttcatgc   3780 

tgcggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa   3840 

atgtctttca gtagcaagct gattgccagg ggcaggccct tggtgtaagt gtttacaaag   3900 

cggttaagct gggatgggtg catacgtggg gatatgagat gcatcttgga ctgtattttt   3960 

aggttggcta tgttcccagc catatccctc cggggattca tgttgtgcag aaccaccagc   4020 

acagtgtatc cggtgcactt gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag   4080 

aacttggaga cgcccttgtg acctccaaga ttttccatgc attcgtccat aatgatggca   4140 

atgggcccac gggcggcggc ctgggcgaag atatttctgg gatcactaac gtcatagttg   4200 

tgttccagga tgagatcgtc ataggccatt tttacaaagc gcgggcggag ggtgccagac   4260 

tgcggtataa tggttccatc cggcccaggg gcgtagttac cctcacagat ttgcatttcc   4320 

cacgctttga gttcagatgg ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt   4380 

tccggggtag gggagatcag ctgggaagaa agcaggttcc tgagcagctg cgacttaccg   4440 

cagccggtgg gcccgtaaat cacacctatt accggctgca actggtagtt aagagagctg   4500 

cagctgccgt catccctgag caggggggcc acttcgttaa gcatgtccct gactcgcatg   4560 

ttttccctga ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag   4620 

gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag gcatgctttt gagcgtttga   4680 

ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct ctacggcatc tcgatccagc   4740 

atatctcctc gtttcgcggg ttggggcggc tttcgctgta cggcagtagt cggtgctcgt   4800 

ccagacgggc cagggtcatg tctttccacg ggcgcagggt cctcgtcagc gtagtctggg   4860 

tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc   4920 

tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag catttgacca   4980 

tggtgtcata gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg cccttggagg   5040 

aggcgccgca cgaggggcag tgcagacttt tgagggcgta gagcttgggc gcgagaaata   5100 

ccgattccgg ggagtaggca tccgcgccgc aggccccgca gacggtctcg cattccacga   5160 

gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc   5220 

gtttcttacc tctggtttcc atgagccggt gtccacgctc ggtgacgaaa aggctgtccg   5280 

tgtccccgta tacagacttg agaggcctgt cctcgagcgg tgttccgcgg tcctcctcgt   5340 

atagaaactc ggaccactct gagacaaagg ctcgcgtcca ggccagcacg aaggaggcta   5400 

agtgggaggg gtagcggtcg ttgtccacta gggggtccac tcgctccagg gtgtgaagac   5460 

acatgtcgcc ctcttcggca tcaaggaagg tgattggttt gtaggtgtag gccacgtgac   5520 

cgggtgttcc tgaagggggg ctataaaagg gggtgggggc gcgttcgtcc tcactctctt   5580 

ccgcatcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctga aaagcgggca   5640 

tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc   5700 

ccgcggtgat gcctttgagg gtggccgcat ccatctggtc agaaaagaca atctttttgt   5760 

tgtcaagctt ggtggcaaac gacccgtaga gggcgttgga cagcaacttg gcgatggagc   5820 

gcagggtttg gtttttgtcg cgatcggcgc gctccttggc cgcgatgttt agctgcacgt   5880 

attcgcgcgc aacgcaccgc cattcgggaa agacggtggt gcgctcgtcg ggcaccaggt   5940 

gcacgcgcca accgcggttg tgcagggtga caaggtcaac gctggtggct acctctccgc   6000 

gtaggcgctc gttggtccag cagaggcggc cgcccttgcg cgagcagaat ggcggtaggg   6060 

ggtctagctg cgtctcgtcc ggggggtctg cgtccacggt aaagaccccg ggcagcaggc   6120 

gcgcgtcgaa gtagtctatc ttgcatcctt gcaagtctag cgcctgctgc catgcgcggg   6180 

cggcaagcgc gcgctcgtat gggttgagtg ggggacccca tggcatgggg tgggtgagcg   6240 

cggaggcgta catgccgcaa atgtcgtaaa cgtagagggg ctctctgagt attccaagat   6300 

atgtagggta gcatcttcca ccgcggatgc tggcgcgcac gtaatcgtat agttcgtgcg   6360 

agggagcgag gaggtcggga ccgaggttgc tacgggcggg ctgctctgct cggaagacta   6420 

tctgcctgaa gatggcatgt gagttggatg atatggttgg acgctggaag acgttgaagc   6480 

tggcgtctgt gagacctacc gcgtcacgca cgaaggaggc gtaggagtcg cgcagcttgt   6540 

tgaccagctc ggcggtgacc tgcacgtcta gggcgcagta gtccagggtt tccttgatga   6600 

tgtcatactt atcctgtccc ttttttttcc acagctcgcg gttgaggaca aactcttcgc   6660 

ggtctttcca gtactcttgg atcggaaacc cgtcggcctc cgaacggtaa gagcctagca   6720 

tgtagaactg gttgacggcc tggtaggcgc agcatccctt ttctacgggt agcgcgtatg   6780 

cctgcgcggc cttccggagc gaggtgtggg tgagcgcaaa ggtgtccctg accatgactt   6840 

tgaggtactg gtatttgaag tcagtgtcgt cgcatccgcc ctgctcccag agcaaaaagt   6900 

ccgtgcgctt tttggaacgc ggatttggca gggcgaaggt gacatcgttg aagagtatct   6960 

ttcccgcgcg aggcataaag ttgcgtgtga tgcggaaggg tcccggcacc tcggaacggt   7020 

tgttaattac ctgggcggcg agcacgatct cgtcaaagcc gttgatgttg tggcccacaa   7080 

tgtaaagttc caagaagcgc gggatgccct tgatggaagg caatttttta agttcctcgt   7140 

aggtgagctc ttcaggggag ctgagcccgt gctctgaaag ggcccagtct gcaagatgag   7200 

ggttggaagc gacgaatgag ctccacaggt cacgggccat tagcatttgc aggtggtcgc   7260 

gaaaggtcct aaactggcga cctatggcca ttttttctgg ggtgatgcag tagaaggtaa   7320 

gcgggtcttg ttcccagcgg tcccatccaa ggttcgcggc taggtctcgc gcggcagtca   7380 

ctagaggctc atctccgccg aacttcatga ccagcatgaa gggcacgagc tgcttcccaa   7440 

aggcccccat ccaagtatag gtctctacat cgtaggtgac aaagagacgc tcggtgcgag   7500 

gatgcgagcc gatcgggaag aactggatct cccgccacca attggaggag tggctattga   7560 

tgtggtgaaa gtagaagtcc ctgcgacggg ccgaacactc gtgctggctt ttgtaaaaac   7620 

gtgcgcagta ctggcagcgg tgcacgggct gtacatcctg cacgaggttg acctgacgac   7680 

cgcgcacaag gaagcagagt gggaatttga gcccctcgcc tggcgggttt ggctggtggt   7740 

cttctacttc ggctgcttgt ccttgaccgt ctggctgctc gaggggagtt acggtggatc   7800 

ggaccaccac gccgcgcgag cccaaagtcc agatgtccgc gcgcggcggt cggagcttga   7860 

tgacaacatc gcgcagatgg gagctgtcca tggtctggag ctcccgcggc gtcaggtcag   7920 

gcgggagctc ctgcaggttt acctcgcata gacgggtcag ggcgcgggct agatccaggt   7980 

gatacctaat ttccaggggc tggttggtgg cggcgtcgat ggcttgcaag aggccgcatc   8040 

cccgcggcgc gactacggta ccgcgcggcg ggcggtgggc cgcgggggtg tccttggatg   8100 

atgcatctaa aagcggtgac gcgggcgagc ccccggaggt agggggggct ccggacccgc   8160 

cgggagaggg ggcaggggca cgtcggcgcc gcgcgcgggc aggagctggt gctgcgcgcg   8220 

taggttgctg gcgaacgcga cgacgcggcg gttgatctcc tgaatctggc gcctctgcgt   8280 

gaagacgacg ggcccggtga gcttgaacct gaaagagagt tcgacagaat caatttcggt   8340 

gtcgttgacg gcggcctggc gcaaaatctc ctgcacgtct cctgagttgt cttgataggc   8400 

gatctcggcc atgaactgct cgatctcttc ctcctggaga tctccgcgtc cggctcgctc   8460 

cacggtggcg gcgaggtcgt tggaaatgcg ggccatgagc tgcgagaagg cgttgaggcc   8520 

tccctcgttc cagacgcggc tgtagaccac gcccccttcg gcatcgcggg cgcgcatgac   8580 

cacctgcgcg agattgagct ccacgtgccg ggcgaagacg gcgtagtttc gcaggcgctg   8640 

aaagaggtag ttgagggtgg tggcggtgtg ttctgccacg aagaagtaca taacccagcg   8700 

tcgcaacgtg gattcgttga tatcccccaa ggcctcaagg cgctccatgg cctcgtagaa   8760 

gtccacggcg aagttgaaaa actgggagtt gcgcgccgac acggttaact cctcctccag   8820 

aagacggatg agctcggcga cagtgtcgcg cacctcgcgc tcaaaggcta caggggcctc   8880 

ttcttcttct tcaatctcct cttccataag ggcctcccct tcttcttctt ctggcggcgg   8940 

tgggggaggg gggacacggc ggcgacgacg gcgcaccggg aggcggtcga caaagcgctc   9000 

gatcatctcc ccgcggcgac ggcgcatggt ctcggtgacg gcgcggccgt tctcgcgggg   9060 

gcgcagttgg aagacgccgc ccgtcatgtc ccggttatgg gttggcgggg ggctgccatg   9120 

cggcagggat acggcgctaa cgatgcatct caacaattgt tgtgtaggta ctccgccgcc   9180 

gagggacctg agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa   9240 

ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg   9300 

gttgtttctg gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg   9360 

gatggtcgac agaagcacca tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc   9420 

catgccccag gcttcgtttt gacatcggcg caggtctttg tagtagtctt gcatgagcct   9480 

ttctaccggc acttcttctt ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc   9540 

ggcggcggcg gagtttggcc gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa   9600 

gcccctcatc ggctgaagca gggctaggtc ggcgacaacg cgctcggcta atatggcctg   9660 

ctgcacctgc gtgagggtag actggaagtc atccatgtcc acaaagcggt ggtatgcgcc   9720 

cgtgttgatg gtgtaagtgc agttggccat aacggaccag ttaacggtct ggtgacccgg   9780 

ctgcgagagc tcggtgtacc tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt   9840 

gcaagtccgc accaggtact ggtatcccac caaaaagtgc ggcggcggct ggcggtagag   9900 

gggccagcgt agggtggccg gggctccggg ggcgagatct tccaacataa ggcgatgata   9960 

tccgtagatg tacctggaca tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa  10020 

gtcgcggacg cggttccaga tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct  10080 

ctggccggtc aggcgcgcgc aatcgttgac gctctagacc gtgcaaaagg agagcctgta  10140 

agcgggcact cttccgtggt ctggtggata aattcgcaag ggtatcatgg cggacgaccg  10200 

gggttcgagc cccgtatccg gccgtccgcc gtgatccatg cggttaccgc ccgcgtgtcg  10260 

aacccaggtg tgcgacgtca gacaacgggg gagtgctcct tttggcttcc ttccaggcgc  10320 

ggcggctgct gcgctagctt ttttggccac tggccgcgcg cagcgtaagc ggttaggctg  10380 

gaaagcgaaa gcattaagtg gctcgctccc tgtagccgga gggttatttt ccaagggttg  10440 

agtcgcggga cccccggttc gagtctcgga ccggccggac tgcggcgaac gggggtttgc  10500 

ctccccgtca tgcaagaccc cgcttgcaaa ttcctccgga aacagggacg agcccctttt  10560 

ttgcttttcc cagatgcatc cggtgctgcg gcagatgcgc ccccctcctc agcagcggca  10620 

agagcaagag cagcggcaga catgcagggc accctcccct cctcctaccg cgtcaggagg  10680 

ggcgacatcc gcggttgacg cggcagcaga tggtgattac gaacccccgc ggcgccgggc  10740 

ccggcactac ctggacttgg aggagggcga gggcctggcg cggctaggag cgccctctcc  10800 

tgagcggcac ccaagggtgc agctgaagcg tgatacgcgt gaggcgtacg tgccgcggca  10860 

gaacctgttt cgcgaccgcg agggagagga gcccgaggag atgcgggatc gaaagttcca  10920 

cgcagggcgc gagctgcggc atggcctgaa tcgcgagcgg ttgctgcgcg aggaggactt  10980 

tgagcccgac gcgcgaaccg ggattagtcc cgcgcgcgca cacgtggcgg ccgccgacct  11040 

ggtaaccgca tacgagcaga cggtgaacca ggagattaac tttcaaaaaa gctttaacaa  11100 

ccacgtgcgt acgcttgtgg cgcgcgagga ggtggctata ggactgatgc atctgtggga  11160 

ctttgtaagc gcgctggagc aaaacccaaa tagcaagccg ctcatggcgc agctgttcct  11220 

tatagtgcag cacagcaggg acaacgaggc attcagggat gcgctgctaa acatagtaga  11280 

gcccgagggc cgctggctgc tcgatttgat aaacatcctg cagagcatag tggtgcagga  11340 

gcgcagcttg agcctggctg acaaggtggc cgccatcaac tattccatgc ttagcctggg  11400 

caagttttac gcccgcaaga tataccatac cccttacgtt cccatagaca aggaggtaaa  11460 

gatcgagggg ttctacatgc gcatggcgct gaaggtgctt accttgagcg acgacctggg  11520 

cgtttatcgc aacgagcgca tccacaaggc cgtgagcgtg agccggcggc gcgagctcag  11580 

cgaccgcgag ctgatgcaca gcctgcaaag ggccctggct ggcacgggca gcggcgatag  11640 

agaggccgag tcctactttg acgcgggcgc tgacctgcgc tgggccccaa gccgacgcgc  11700 

cctggaggca gctggggccg gacctgggct ggcggtggca cccgcgcgcg ctggcaacgt  11760 

cggcggcgtg gaggaatatg acgaggacga tgagtacgag ccagaggacg gcgagtacta  11820 

agcggtgatg tttctgatca gatgatgcaa gacgcaacgg acccggcggt gcgggcggcg  11880 

ctgcagagcc agccgtccgg ccttaactcc acggacgact ggcgccaggt catggaccgc  11940 

atcatgtcgc tgactgcgcg caatcctgac gcgttccggc agcagccgca ggccaaccgg  12000 

ctctccgcaa ttctggaagc ggtggtcccg gcgcgcgcaa accccacgca cgagaaggtg  12060 

ctggcgatcg taaacgcgct ggccgaaaac agggccatcc ggcccgacga ggccggcctg  12120 

gtctacgacg cgctgcttca gcgcgtggct cgttacaaca gcggcaacgt gcagaccaac  12180 

ctggaccggc tggtggggga tgtgcgcgag gccgtggcgc agcgtgagcg cgcgcagcag  12240 

cagggcaacc tgggctccat ggttgcacta aacgccttcc tgagtacaca gcccgccaac  12300 

gtgccgcggg gacaggagga ctacaccaac tttgtgagcg cactgcggct aatggtgact  12360 

gagacaccgc aaagtgaggt gtaccagtct gggccagact attttttcca gaccagtaga  12420 

caaggcctgc agaccgtaaa cctgagccag gctttcaaaa acttgcaggg gctgtggggg  12480 

gtgcgggctc ccacaggcga ccgcgcgacc gtgtctagct tgctgacgcc caactcgcgc  12540 

ctgttgctgc tgctaatagc gcccttcacg gacagtggca gcgtgtcccg ggacacatac  12600 

ctaggtcact tgctgacact gtaccgcgag gccataggtc aggcgcatgt ggacgagcat  12660 

actttccagg agattacaag tgtcagccgc gcgctggggc aggaggacac gggcagcctg  12720 

gaggcaaccc taaactacct gctgaccaac cggcggcaga agatcccctc gttgcacagt  12780 

ttaaacagcg aggaggagcg cattttgcgc tacgtgcagc agagcgtgag ccttaacctg  12840 

atgcgcgacg gggtaacgcc cagcgtggcg ctggacatga ccgcgcgcaa catggaaccg  12900 

ggcatgtatg cctcaaaccg gccgtttatc aaccgcctaa tggactactt gcatcgcgcg  12960 

gccgccgtga accccgagta tttcaccaat gccatcttga acccgcactg gctaccgccc  13020 

cctggtttct acaccggggg attcgaggtg cccgagggta acgatggatt cctctgggac  13080 

gacatagacg acagcgtgtt ttccccgcaa ccgcagaccc tgctagagtt gcaacagcgc  13140 

gagcaggcag aggcggcgct gcgaaaggaa agcttccgca ggccaagcag cttgtccgat  13200 

ctaggcgctg cggccccgcg gtcagatgct agtagcccat ttccaagctt gatagggtct  13260 

cttaccagca ctcgcaccac ccgcccgcgc ctgctgggcg aggaggagta cctaaacaac  13320 

tcgctgctgc agccgcagcg cgaaaaaaac ctgcctccgg catttcccaa caacgggata  13380 

gagagcctag tggacaagat gagtagatgg aagacgtacg cgcaggagca cagggacgtg  13440 

ccaggcccgc gcccgcccac ccgtcgtcaa aggcacgacc gtcagcgggg tctggtgtgg  13500 

gaggacgatg actcggcaga cgacagcagc gtcctggatt tgggagggag tggcaacccg  13560 

tttgcgcacc ttcgccccag gctggggaga atgttttaaa aaaaaaaaaa gcatgatgca  13620 

aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt  13680 

atgcggcgcg cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc  13740 

gcggcgccag tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt  13800 

gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg  13860 

gcacccctat tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca  13920 

tccctgaact accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac  13980 

tacagcccgg gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc  14040 

ggcgacctga aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc  14100 

aataagttta aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag  14160 

ctgaaatacg agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc  14220 

atagacctta tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg  14280 

gttctggaaa gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac  14340 

cccgtcactg gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc  14400 

attttgctgc caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc  14460 

atccgcaagc ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt  14520 

ggtaacattc ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc  14580 

gaacagggcg ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac  14640 

tccaacgcgg cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc  14700 

ggcgacacct ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa  14760 

gctgccgccc ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa  14820 

cccctgacag aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc  14880 

acccagtacc gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc  14940 

tcatggaccc tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg  15000 

ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt  15060 

ccggtggtgg gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc  15120 

gtctactccc aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc  15180 

gagaaccaga ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt  15240 

cctgctctca cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga  15300 

gtgaccatta ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata  15360 

gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg  15420 

cccagcaata acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag  15480 

aagcgctccg accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg  15540 

cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag  15600 

gaggcgcgca actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag  15660 

accgtggtgc gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca  15720 

cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac  15780 

cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt  15840 

attgtcactg tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt  15900 

agtgctatga ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc  15960 

ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta  16020 

gactcgtact gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc  16080 

aaaatcaaag aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag  16140 

gaagagcagg attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat  16200 

gatgatgaac ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta  16260 

cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg  16320 

cccggtgagc gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag  16380 

gacctgcttg agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag  16440 

gacatgctgg cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca  16500 

ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag  16560 

tctggtgact tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat  16620 

gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc  16680 

aagcaggtgg cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt  16740 

agcaccagta ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca  16800 

gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag  16860 

gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg  16920 

aagtacggcg ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct  16980 

acccccggct atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga  17040 

accaccactg gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc  17100 

gtgcgcaggg tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac  17160 

cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc  17220 

ctccgtttcc cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc  17280 

cacggcctga cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt  17340 

cgcatgcgcg gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc  17400 

gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc  17460 

atgtggaaaa atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt  17520 

ttgtagaatg gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt  17580 

catgggaaac tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg  17640 

ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc  17700 

ctggaacagc agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca  17760 

aaaggtggta gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc  17820 

agtgcaaaat aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc  17880 

ggccgtggag acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga  17940 

agaaactctg gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg  18000 

cctgcccacc acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc  18060 

cgtaacgctg gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc  18120 

gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc  18180 

gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg  18240 

tctgggggtg caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg  18300 

tgtcatgtat gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt  18360 

ccaagatggc taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg  18420 

acgcctcgga gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact  18480 

tcagcctgaa taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag  18540 

accggtccca gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact  18600 

cgtacaaggc gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca  18660 

cgtactttga catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca  18720 

ctgcctacaa cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg  18780 

ctactgctct tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg  18840 

agcaagctga gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata  18900 

ttacaaagga gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa  18960 

catttcaacc tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg  19020 

cagctgggag agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa  19080 

aacccacaaa tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag  19140 

aaagtcaagt ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata  19200 

acttgactcc taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc  19260 

atatttctta catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat  19320 

ctatgcccaa caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt  19380 

acaacagcac gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg  19440 

tagatttgca agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg  19500 

atagaaccag gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta  19560 

gaattattga aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag  19620 

gtgtgattaa tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat  19680 

gggaaaaaga tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg  19740 

ccatggaaat caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc  19800 

tgtatttgcc cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa  19860 

acacctacga ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta  19920 

accttggagc acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc  19980 

gcaatgctgg cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc  20040 

acatccaggt gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat  20100 

acacctacga gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa  20160 

atgacctaag ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct  20220 

tcttccccat ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca  20280 

acgaccagtc ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca  20340 

acgctaccaa cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg  20400 

ccttcacgcg ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt  20460 

acacctactc tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta  20520 

agaaggtggc cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta  20580 

cccccaacga gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt  20640 

gtaacatgac caaagactgg ttcctggtac aaatgctagc taactataac attggctacc  20700 

agggcttcta tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc  20760 

agcccatgag ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca  20820 

tcctacacca acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag  20880 

gacaggccta ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca  20940 

ttacccagaa aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact  21000 

ttatgtccat gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc  21060 

acgcgctaga catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt  21120 

tgtttgaagt ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg  21180 

tgtacctgcg cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc  21240 

aacaacagct gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct  21300 

tggttgtggg ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc  21360 

acacaagctc gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg  21420 

gatggccttt gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt  21480 

ttctgaccag cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag  21540 

cgccattgct tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca  21600 

ggggcccaac tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa  21660 

ctggccccaa actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa  21720 

ctccatgctc aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta  21780 

cagcttcctg gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc  21840 

cacttctttt tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa  21900 

aggcaaatgc ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg  21960 

cgccgtttaa aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac  22020 

gttgcgatac tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc  22080 

ggtgaagttt tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc  22140 

cgatatcttg aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg  22200 

gttgcagcac tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc  22260 

ggagatcaga tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg  22320 

tagctgcctt cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg  22380 

catcaaaagg tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt  22440 

gatctgctta aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt  22500 

gccggaaaac tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt  22560 

ggagatctgc accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg  22620 

ctccttcagc gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt  22680 

atttatcata atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg  22740 

cagccacaac gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg  22800 

caggtacgcc tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt  22860 

cagctgcaac ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc  22920 

cacttggtca ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc  22980 

catcagcgcg cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag  23040 

cgggttcatc accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt  23100 

ccgcatacca cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc  23160 

tttgccatgc ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc  23220 

ttctctttct tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg  23280 

agaagggcgc ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg  23340 

ccgcgggctg ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga  23400 

ctcgatacgc cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga  23460 

cggggacgac acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg  23520 

ggtggtttcg cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa  23580 

gatcatggag tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac  23640 

cgcctccacc gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga  23700 

ggaggaggaa gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg  23760 

ctcagtacca acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca  23820 

agtcgggcgg ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt  23880 

gaagcatctg cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt  23940 

gcccctcgcc atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt  24000 

accccccaaa cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc  24060 

cgtatttgcc gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat  24120 

acccctatcc tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg  24180 

cgctgtcata cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg  24240 

acgcgacgag aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca  24300 

ctctggagtg ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag  24360 

catcgaggtc acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac  24420 

agtcatgagt gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca  24480 

agaacaaaca gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca  24540 

aacgcgcgag cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt  24600 

taccgtggag cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct  24660 

agaggaaaca ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc  24720 

caacgtggag ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg  24780 

gcaaaacgtg cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg  24840 

cgtttactta tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt  24900 

ggaggagtgc aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg  24960 

gacggccttc aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg  25020 

cctgcttaaa accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa  25080 

ctttaggaac tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc  25140 

tagcgacttt gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta  25200 

ccttctgcag ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg  25260 

tgacggtcta ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt  25320 

ttgcaattcg cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc  25380 

ctcgcctgac gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc  25440 

ggcttacctt cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga  25500 

agaccaatcc cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat  25560 

tcttggccaa ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg  25620 

gggggtttac ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca  25680 

gccctatcag cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc  25740 

agctgccgcc gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt  25800 

tggacgagga ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg  25860 

aggtcgaaga ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc  25920 

cccagaaatc ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg  25980 

cactgcccgt tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt  26040 

ccaagcagcc gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc  26100 

gcgggcacaa gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg  26160 

cccgccgctt tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact  26220 

accgtcatct ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca  26280 

cagaagcaaa ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg  26340 

gcagcagcag gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag  26400 

cttagaaaca ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa  26460 

caagagctga aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac  26520 

aaaagcgaag atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac  26580 

tgcgcgctga ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta  26640 

cgtcatctcc agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag  26700 

gaaattccca cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct  26760 

gcccaagact actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg  26820 

gtcaacggaa tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc  26880 

acacctcgta ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt  26940 

cccgctccca ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac  27000 

tcaggggcgc agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata  27060 

actcacctga caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg  27120 

cttggtctcc gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg  27180 

cctcgtcagg caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt  27240 

ggaactctgc aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga  27300 

cctcccggcc actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg  27360 

gacggctacg actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc  27420 

cactgtcgcc gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg  27480 

cccgaggatc atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt  27540 

gcccgtagcc tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga  27600 

ccctgtgttc tcactgtgat ttgcaactgt cctaaccctg gattacatca agatctttgt  27660 

tgccatctct gtgctgagta taataaatac agaaattaaa atatactggg gctcctatcg  27720 

ccatcctgta aacgccaccg tcttcacccg cccaagcaaa ccaaggcgaa ccttacctgg  27780 

tacttttaac atctctccct ctgtgattta caacagtttc aacccagacg gagtgagtct  27840 

acgagagaac ctctccgagc tcagctactc catcagaaaa aacaccaccc tccttacctg  27900 

ccgggaacgt acgagtgcgt caccggccgc tgcaccacac ctaccgcctg accgtaaacc  27960 

agactttttc cggacagacc tcaataactc tgtttaccag aacaggaggt gagcttagaa  28020 

aacccttagg gtattaggcc aaaggcgcag ctactgtggg gtttatgaac aattcaagca  28080 

actctacggg ctattctaat tcaggtttct ctagaatcgg ggttggggtt attctctgtc  28140 

ttgtgattct ctttattctt atactaacgc ttctctgcct aaggctcgcc gcctgctgtg  28200 

tgcacatttg catttattgt cagcttttta aacgctgggg tcgccaccca agatgattag  28260 

gtacataatc ctaggtttac tcacccttgc gtcagcccac ggtaccaccc aaaaggtgga  28320 

ttttaaggag ccagcctgta atgttacatt cgcagctgaa gctaatgagt gcaccactct  28380 

tataaaatgc accacagaac atgaaaagct gcttattcgc cacaaaaaca aaattggcaa  28440 

gtatgctgtt tatgctattt ggcagccagg tgacactaca gagtataatg ttacagtttt  28500 

ccagggtaaa agtcataaaa cttttatgta tacttttcca ttttatgaaa tgtgcgacat  28560 

taccatgtac atgagcaaac agtataagtt gtggccccca caaaattgtg tggaaaacac  28620 

tggcactttc tgctgcactg ctatgctaat tacagtgctc gctttggtct gtaccctact  28680 

ctatattaaa tacaaaagca gacgcagctt tattgaggaa aagaaaatgc cttaatttac  28740 

taagttacaa agctaatgtc accactaact gctttactcg ctgcttgcaa aacaaattca  28800 

aaaagttagc attataatta gaataggatt taaacccccc ggtcatttcc tgctcaatac  28860 

cattcccctg aacaattgac tctatgtggg atatgctcca gcgctacaac cttgaagtca  28920 

ggcttcctgg atgtcagcat ctgactttgg ccagcacctg tcccgcggat ttgttccagt  28980 

ccaactacag cgacccaccc taacagagat gaccaacaca accaacgcgg ccgccgctac  29040 

cggacttaca tctaccacaa atacacccca agtttctgcc tttgtcaata actgggataa  29100 

cttgggcatg tggtggttct ccatagcgct tatgtttgta tgccttatta ttatgtggct  29160 

catctgctgc ctaaagcgca aacgcgcccg accacccatc tatagtccca tcattgtgct  29220 

acacccaaac aatgatggaa tccatagatt ggacggactg aaacacatgt tcttttctct  29280 

tacagtatga ttaaatgaga catgattcct cgagttttta tattactgac ccttgttgcg  29340 

cttttttgtg cgtgctccac attggctgcg gtttctcaca tcgaagtaga ctgcattcca  29400 

gccttcacag tctatttgct ttacggattt gtcaccctca cgctcatctg cagcctcatc  29460 

actgtggtca tcgcctttat ccagtgcatt gactgggtct gtgtgcgctt tgcatatctc  29520 

agacaccatc cccagtacag ggacaggact atagctgagc ttcttagaat tctttaatta  29580 

tgaaatttac tgtgactttt ctgctgatta tttgcaccct atctgcgttt tgttccccga  29640 

cctccaagcc tcaaagacat atatcatgca gattcactcg tatatggaat attccaagtt  29700 

gctacaatga aaaaagcgat ctttccgaag cctggttata tgcaatcatc tctgttatgg  29760 

tgttctgcag taccatctta gccctagcta tatatcccta ccttgacatt ggctggaacg  29820 

caatagatgc catgaaccac ccaactttcc ccgcgcccgc tatgcttcca ctgcaacaag  29880 

ttgttgccgg cggctttgtc ccagccaatc agcctcgccc accttctccc acccccactg  29940 

aaatcagcta ctttaatcta acaggaggag atgactgaca ccctagatct agaaatggac  30000 

ggaattatta cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc  30060 

atgaatcaag agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt  30120 

ctcgtaaagc aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac  30180 

aagttgccaa ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata  30240 

actcagcact cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat  30300 

ctctgcaccc ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa  30360 

aaaaaaataa taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt  30420 

cagcagcacc tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa  30480 

ctttctccac aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac  30540 

tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt  30600 

gtatccatat gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt  30660 

atcccccaat gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc  30720 

tctagttacc tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga  30780 

ggccggcaac cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa  30840 

gtcaaacata aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt  30900 

ggctgccgcc gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc  30960 

gctaaccgtg cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga  31020 

aggaaagcta gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac  31080 

tatcactgcc tcaccccctt taactactgc cactggtagc ttgggcattg acttgaaaga  31140 

gcccatttat acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac  31200 

agacgaccta aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc  31260 

cttgcaaact aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa  31320 

tgtagcagga ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta  31380 

tccgtttgat gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa  31440 

ctcagcccac aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa  31500 

caattccaaa aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac  31560 

agccatagcc attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac  31620 

aaatcccctc aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt  31680 

tcctaaacta ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa  31740 

aaataatgat aagctaactt tgtggaccac accagctcca tctcctaact gtagactaaa  31800 

tgcagagaaa gatgctaaac tcactttggt cttaacaaaa tgtggcagtc aaatacttgc  31860 

tacagtttca gttttggctg ttaaaggcag tttggctcca atatctggaa cagttcaaag  31920 

tgctcatctt attataagat ttgacgaaaa tggagtgcta ctaaacaatt ccttcctgga  31980 

cccagaatat tggaacttta gaaatggaga tcttactgaa ggcacagcct atacaaacgc  32040 

tgttggattt atgcctaacc tatcagctta tccaaaatct cacggtaaaa ctgccaaaag  32100 

taacattgtc agtcaagttt acttaaacgg agacaaaact aaacctgtaa cactaaccat  32160 

tacactaaac ggtacacagg aaacaggaga cacaactcca agtgcatact ctatgtcatt  32220 

ttcatgggac tggtctggcc acaactacat taatgaaata tttgccacat cctcttacac  32280 

tttttcatac attgcccaag aataaagaat cgtttgtgtt atgtttcaac gtgtttattt  32340 

ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata  32400 

gcttatacag atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac  32460 

ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca  32520 

tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca  32580 

aacgctcatc agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt  32640 

ccagctgctg agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag  32700 

aagtccacgc ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct  32760 

gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg  32820 

cagtggtctc ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg  32880 

cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa  32940 

tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag  33000 

aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca  33060 

cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata  33120 

taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct  33180 

gcccgccggc tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg  33240 

actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca  33300 

cgtgcataca cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa  33360 

caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca  33420 

cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag  33480 

cgcgggtttc tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca  33540 

accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc  33600 

ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta  33660 

gatcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg  33720 

gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca  33780 

gaataagcca cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg  33840 

ggaagagctg gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa  33900 

atgaagatct attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa  33960 

agaacagata atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct  34020 

cacgtccaag tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc  34080 

agcaccttca accatgccca aataattctc atctcgccac cttctcaata tatctctaag  34140 

caaatcccga atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt  34200 

cagcctcaag cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga  34260 

ttcaaaagcg gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc  34320 

tgaacataat cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg  34380 

acaaaagaac ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc  34440 

ccgatgtaag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca  34500 

ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag  34560 

gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt  34620 

ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta  34680 

caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt  34740 

aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag  34800 

tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc  34860 

gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca  34920 

taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct  34980 

gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag  35040 

ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac  35100 

ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag  35160 

gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa  35220 

cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt  35280 

tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac  35340 

tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc  35400 

caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa  35460 

ttaagaattc ggatctgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc  35520 

ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga  35580 

ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct  35640 

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca  35700 

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct  35760 

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc  35820 

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt  35880 

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc  35940 

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc  36000 

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg  36060 

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc  36120 

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag  36180 

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga  36240 

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat  36300 

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatc aatctaaagt  36360 

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca  36420 

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg  36480 

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca  36540 

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt  36600 

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt  36660 

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca  36720 

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca  36780 

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga  36840 

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact  36900 

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga  36960 

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg  37020 

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc  37080 

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga  37140 

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat  37200 

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt  37260 

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt  37320 

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac  37380 

gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc  37440 

tttcgtcttc aagaattgga tccgaattct taat                              37474 

 
           
             7  
             37474  
             DNA  
             Artificial Sequence  
             
               pMRKAd5HIV-1 gag, non-coding  
             
           
            7 

aagaattaat tgtagtagtt attatatgga ataaaaccta acttcggtta tactattact     60 

cccccacctc aaacactgca ccgcgccccg cacccttgcc ccgcccactg catcatcaca    120 

ccgccttcac actacaacgt tcacaccgcc ttgtgtacat tcgctgccta caccgttttc    180 

actgcaaaaa ccacacgcgg ccacatgtgt ccttcactgt taaaagcgcg ccaaaatccg    240 

cctacaacat catttaaacc cgcattggct cattctaaac cggtaaaagc gcccttttga    300 

cttattctcc ttcactttag acttattaaa acacaatgag tatcgcgcat tataaacaga    360 

tcccggcgcc cctgaaactg gcaaatgcac ctctgagcgg gtccacaaaa agagtccaca    420 

aaaggcgcaa ggcccagttt caaccgcaaa ataataatat ccgccggcgc taggtaacgt    480 

atgcaacata ggtatagtat tatacatgta aatataaccg agtacaggtt gtaatggcgg    540 

tacaactgta actaataact gatcaataat tatcattagt taatgcccca gtaatcaagt    600 

atcgggtata tacctcaagg cgcaatgtat tgaatgccat ttaccgggcg gaccgactgg    660 

cgggttgctg ggggcgggta actgcagtta ttactgcata caagggtatc attgcggtta    720 

tccctgaaag gtaactgcag ttacccacct cataaatgcc atttgacggg tgaaccgtca    780 

tgtagttcac atagtatacg gttcatgcgg gggataactg cagttactgc catttaccgg    840 

gcggaccgta atacgggtca tgtactggaa taccctgaaa ggatgaaccg tcatgtagat    900 

gcataatcag tagcgataat ggtaccacta cgccaaaacc gtcatgtagt tacccgcacc    960 

tatcgccaaa ctgagtgccc ctaaaggttc agaggtgggg taactgcagt taccctcaaa   1020 

caaaaccgtg gttttagttg ccctgaaagg ttttacagca ttgttgaggc ggggtaactg   1080 

cgtttacccg ccatccgcac atgccaccct ccagatatat tcgtctcgag caaatcactt   1140 

ggcagtctag cggacctctg cggtaggtgc gacaaaactg gaggtatctt ctgtggccct   1200 

ggctaggtcg gaggcgccgg cccttgccac gtaaccttgc gcctaagggg cacggttctc   1260 

actctagatg gtacccacga tcccgaagac acgacagacc accactcgac ctgttcaccc   1320 

tcttctagtc cgactccgga ccaccgttct tcttcatgtt cgatttcgtg taacacaccc   1380 

ggaggtccct cgacctctcc aaacgacact tgggaccgga cgacctctgg agactcccca   1440 

cgtccgtcta ggacccggtc gaggtcggga gggacgtttg tccgagactc ctcgactcca   1500 

gggacatgtt gtgtcaccga tgggacatga cacacgtggt cttctaacta cacttcctgt   1560 

ggttcctccg ggacctcttc taactcctcc tcgtcttgtt caggttcttc ttccgggtcg   1620 

tccgacgacg accgtgtccg ttgaggtcgg tccacagggt cttgatgggg taacacgtct   1680 

tggaggtccc ggtctaccac gtggtccggt agaggggggc ctgggactta cggacccact   1740 

tccaccacct cctcttccgg aagaggggac tccactaggg gtacaagaga cgggacagac   1800 

tcccacggtg gggggtcctg gacttgtggt acgacttgtg tcaccccccg gtagtccgac   1860 

ggtacgtcta cgacttcctc tggtagttac tcctccgacg actcaccctg tccgacgtag   1920 

gacacgtgcg accggggtaa cgggggccgg tctactccct cgggtccccg agactgtaac   1980 

gaccgtggtg gaggtgggag gtcctcgtct aaccgaccta ctggttgttg ggggggtagg   2040 

gacaccccct ttagatgttc tccacctagt aggacccgga cttgttctaa cactcctaca   2100 

tgagggggtg gaggtaggac ctgtagtccg tcccggggtt cctcgggaag tccctgatac   2160 

acctgtccaa gatgttctgg gactcccgac tcgtccggag ggtcctccac ttcttgacct   2220 

actgtctctg ggacgaccac gtcttacggt tgggactgac gttctggtag gacttccggg   2280 

acccgggacg acggtgggac ctcctctact actgtcggac ggtcccccac cccccgggac   2340 

cagtgttccg gtcccacgac cgactccggt acagggtcca ctggttgagg cggtggtagt   2400 

actacgtctc cccgttgaag tccttggtct ccttctgtca cttcacgaag ttgacaccgt   2460 

tccacccggt gtaacggttc ttgacatccc gggggtcctt cttcccgacg accttcacac   2520 

cgttcctccc ggtggtctac ttcctgacgt tactctccgt ccggttgaag gacccgtttt   2580 

agaccgggag ggtgttcccg tccggaccgt tgaaggaggt caggtccgga ctcgggtgtc   2640 

ggggagggct cctcaggaag tccaaacccc tcctcttctg gtgggggtcg gtcttcgtcc   2700 

tcgggtaact gttcctcgac atgggggacc ggagggactc cagggacaaa ccgttgctgg   2760 

ggaggagggt cattttattt cgggcccgtc tagacgacac ggaagatcaa cggtcggtag   2820 

acaacaaacg gggagggggc acggaaggaa ctgggacctt ccacggtgag ggtgacagga   2880 

aaggattatt ttactccttt aacgtagcgt aacagactca tccacagtaa gataagaccc   2940 

cccaccccac cccgtcctgt cgttccccct cctaaccctt ctgttatcgt ccgtacgacc   3000 

cctacgccac ccgagatacc ggctagccgc gcggcatgac tttacacacc cgcaccgaat   3060 

tcccaccctt tcttatatat tccaccccca gaatacatca aaacatagac aaaacgtcgt   3120 

cggcggcggc ggtactcgtg gttgagcaaa ctaccttcgt aacactcgag tataaactgt   3180 

tgcgcgtacg ggggtacccg gccccacgca gtcttacact acccgaggtc gtaactacca   3240 

gcggggcagg acgggcgttt gagatgatgg aactggatgc tctggcacag accttgcggc   3300 

aacctctgac gtcggaggcg gcggcgaagt cggcgacgtc ggtggcgggc gccctaacac   3360 

tgactgaaac gaaaggactc gggcgaacgt ttgtcacgtc gaagggcaag taggcgggcg   3420 

ctactgttca actgccgaga aaaccgtgtt aacctaagaa actgggccct tgaattacag   3480 

caaagagtcg tcgacaacct agacgcggtc gtccaaagac gggacttccg aaggagggga   3540 

gggttacgcc aaattttgta tttatttttt ggtctgagac aaacctaaac ctagttcgtt   3600 

cacagaacga cagaaataaa tccccaaaac gcgcgcgcca tccgggccct ggtcgccaga   3660 

gccagcaact cccaggacac ataaaaaagg tcctgcacca tttccactga gacctacaag   3720 

tctatgtacc cgtattcggg cagagacccc acctccatcg tggtgacgtc tcgaagtacg   3780 

acgccccacc acaacatcta ctaggtcagc atcgtcctcg cgacccgcac cacggatttt   3840 

tacagaaagt catcgttcga ctaacggtcc ccgtccggga accacattca caaatgtttc   3900 

gccaattcga ccctacccac gtatgcaccc ctatactcta cgtagaacct gacataaaaa   3960 

tccaaccgat acaagggtcg gtatagggag gcccctaagt acaacacgtc ttggtggtcg   4020 

tgtcacatag gccacgtgaa ccctttaaac agtacatcga atcttccttt acgcaccttc   4080 

ttgaacctct gcgggaacac tggaggttct aaaaggtacg taagcaggta ttactaccgt   4140 

tacccgggtg cccgccgccg gacccgcttc tataaagacc ctagtgattg cagtatcaac   4200 

acaaggtcct actctagcag tatccggtaa aaatgtttcg cgcccgcctc ccacggtctg   4260 

acgccatatt accaaggtag gccgggtccc cgcatcaatg ggagtgtcta aacgtaaagg   4320 

gtgcgaaact caagtctacc cccctagtac agatggacgc cccgctactt cttttgccaa   4380 

aggccccatc ccctctagtc gacccttctt tcgtccaagg actcgtcgac gctgaatggc   4440 

gtcggccacc cgggcattta gtgtggataa tggccgacgt tgaccatcaa ttctctcgac   4500 

gtcgacggca gtagggactc gtccccccgg tgaagcaatt cgtacaggga ctgagcgtac   4560 

aaaagggact ggtttaggcg gtcttccgcg agcggcgggt cgctatcgtc aagaacgttc   4620 

cttcgtttca aaaagttgcc aaactctggc aggcggcatc cgtacgaaaa ctcgcaaact   4680 

ggttcgtcaa ggtccgccag ggtgtcgagc cagtggacga gatgccgtag agctaggtcg   4740 

tatagaggag caaagcgccc aaccccgccg aaagcgacat gccgtcatca gccacgagca   4800 

ggtctgcccg gtcccagtac agaaaggtgc ccgcgtccca ggagcagtcg catcagaccc   4860 

agtgccactt ccccacgcga ggcccgacgc gcgaccggtc ccacgcgaac tccgaccagg   4920 

acgaccacga cttcgcgacg gccagaagcg ggacgcgcag ccggtccatc gtaaactggt   4980 

accacagtat caggtcgggg aggcgccgca ccgggaaccg cgcgtcgaac gggaacctcc   5040 

tccgcggcgt gctccccgtc acgtctgaaa actcccgcat ctcgaacccg cgctctttat   5100 

ggctaaggcc cctcatccgt aggcgcggcg tccggggcgt ctgccagagc gtaaggtgct   5160 

cggtccactc gagaccggca agccccagtt tttggtccaa agggggtacg aaaaactacg   5220 

caaagaatgg agaccaaagg tactcggcca caggtgcgag ccactgcttt tccgacaggc   5280 

acaggggcat atgtctgaac tctccggaca ggagctcgcc acaaggcgcc aggaggagca   5340 

tatctttgag cctggtgaga ctctgtttcc gagcgcaggt ccggtcgtgc ttcctccgat   5400 

tcaccctccc catcgccagc aacaggtgat cccccaggtg agcgaggtcc cacacttctg   5460 

tgtacagcgg gagaagccgt agttccttcc actaaccaaa catccacatc cggtgcactg   5520 

gcccacaagg acttcccccc gatattttcc cccacccccg cgcaagcagg agtgagagaa   5580 

ggcgtagcga cagacgctcc cggtcgacaa ccccactcat gagggagact tttcgcccgt   5640 

actgaagacg cgattctaac agtcaaaggt ttttgctcct cctaaactat aagtggaccg   5700 

ggcgccacta cggaaactcc caccggcgta ggtagaccag tcttttctgt tagaaaaaca   5760 

acagttcgaa ccaccgtttg ctgggcatct cccgcaacct gtcgttgaac cgctacctcg   5820 

cgtcccaaac caaaaacagc gctagccgcg cgaggaaccg gcgctacaaa tcgacgtgca   5880 

taagcgcgcg ttgcgtggcg gtaagccctt tctgccacca cgcgagcagc ccgtggtcca   5940 

cgtgcgcggt tggcgccaac acgtcccact gttccagttg cgaccaccga tggagaggcg   6000 

catccgcgag caaccaggtc gtctccgccg gcgggaacgc gctcgtctta ccgccatccc   6060 

ccagatcgac gcagagcagg ccccccagac gcaggtgcca tttctggggc ccgtcgtccg   6120 

cgcgcagctt catcagatag aacgtaggaa cgttcagatc gcggacgacg gtacgcgccc   6180 

gccgttcgcg cgcgagcata cccaactcac cccctggggt accgtacccc acccactcgc   6240 

gcctccgcat gtacggcgtt tacagcattt gcatctcccc gagagactca taaggttcta   6300 

tacatcccat cgtagaaggt ggcgcctacg accgcgcgtg cattagcata tcaagcacgc   6360 

tccctcgctc ctccagccct ggctccaacg atgcccgccc gacgagacga gccttctgat   6420 

agacggactt ctaccgtaca ctcaacctac tataccaacc tgcgaccttc tgcaacttcg   6480 

accgcagaca ctctggatgg cgcagtgcgt gcttcctccg catcctcagc gcgtcgaaca   6540 

actggtcgag ccgccactgg acgtgcagat cccgcgtcat caggtcccaa aggaactact   6600 

acagtatgaa taggacaggg aaaaaaaagg tgtcgagcgc caactcctgt ttgagaagcg   6660 

ccagaaaggt catgagaacc tagcctttgg gcagccggag gcttgccatt ctcggatcgt   6720 

acatcttgac caactgccgg accatccgcg tcgtagggaa aagatgccca tcgcgcatac   6780 

ggacgcgccg gaaggcctcg ctccacaccc actcgcgttt ccacagggac tggtactgaa   6840 

actccatgac cataaacttc agtcacagca gcgtaggcgg gacgagggtc tcgtttttca   6900 

ggcacgcgaa aaaccttgcg cctaaaccgt cccgcttcca ctgtagcaac ttctcataga   6960 

aagggcgcgc tccgtatttc aacgcacact acgccttccc agggccgtgg agccttgcca   7020 

acaattaatg gacccgccgc tcgtgctaga gcagtttcgg caactacaac accgggtgtt   7080 

acatttcaag gttcttcgcg ccctacggga actaccttcc gttaaaaaat tcaaggagca   7140 

tccactcgag aagtcccctc gactcgggca cgagactttc ccgggtcaga cgttctactc   7200 

ccaaccttcg ctgcttactc gaggtgtcca gtgcccggta atcgtaaacg tccaccagcg   7260 

ctttccagga tttgaccgct ggataccggt aaaaaagacc ccactacgtc atcttccatt   7320 

cgcccagaac aagggtcgcc agggtaggtt ccaagcgccg atccagagcg cgccgtcagt   7380 

gatctccgag tagaggcggc ttgaagtact ggtcgtactt cccgtgctcg acgaagggtt   7440 

tccgggggta ggttcatatc cagagatgta gcatccactg tttctctgcg agccacgctc   7500 

ctacgctcgg ctagcccttc ttgacctaga gggcggtggt taacctcctc accgataact   7560 

acaccacttt catcttcagg gacgctgccc ggcttgtgag cacgaccgaa aacatttttg   7620 

cacgcgtcat gaccgtcgcc acgtgcccga catgtaggac gtgctccaac tggactgctg   7680 

gcgcgtgttc cttcgtctca cccttaaact cggggagcgg accgcccaaa ccgaccacca   7740 

gaagatgaag ccgacgaaca ggaactggca gaccgacgag ctcccctcaa tgccacctag   7800 

cctggtggtg cggcgcgctc gggtttcagg tctacaggcg cgcgccgcca gcctcgaact   7860 

actgttgtag cgcgtctacc ctcgacaggt accagacctc gagggcgccg cagtccagtc   7920 

cgccctcgag gacgtccaaa tggagcgtat ctgcccagtc ccgcgcccga tctaggtcca   7980 

ctatggatta aaggtccccg accaaccacc gccgcagcta ccgaacgttc tccggcgtag   8040 

gggcgccgcg ctgatgccat ggcgcgccgc ccgccacccg gcgcccccac aggaacctac   8100 

tacgtagatt ttcgccactg cgcccgctcg ggggcctcca tcccccccga ggcctgggcg   8160 

gccctctccc ccgtccccgt gcagccgcgg cgcgcgcccg tcctcgacca cgacgcgcgc   8220 

atccaacgac cgcttgcgct gctgcgccgc caactagagg acttagaccg cggagacgca   8280 

cttctgctgc ccgggccact cgaacttgga ctttctctca agctgtctta gttaaagcca   8340 

cagcaactgc cgccggaccg cgttttagag gacgtgcaga ggactcaaca gaactatccg   8400 

ctagagccgg tacttgacga gctagagaag gaggacctct agaggcgcag gccgagcgag   8460 

gtgccaccgc cgctccagca acctttacgc ccggtactcg acgctcttcc gcaactccgg   8520 

agggagcaag gtctgcgccg acatctggtg cgggggaagc cgtagcgccc gcgcgtactg   8580 

gtggacgcgc tctaactcga ggtgcacggc ccgcttctgc cgcatcaaag cgtccgcgac   8640 

tttctccatc aactcccacc accgccacac aagacggtgc ttcttcatgt attgggtcgc   8700 

agcgttgcac ctaagcaact atagggggtt ccggagttcc gcgaggtacc ggagcatctt   8760 

caggtgccgc ttcaactttt tgaccctcaa cgcgcggctg tgccaattga ggaggaggtc   8820 

ttctgcctac tcgagccgct gtcacagcgc gtggagcgcg agtttccgat gtccccggag   8880 

aagaagaaga agttagagga gaaggtattc ccggagggga agaagaagaa gaccgccgcc   8940 

accccctccc ccctgtgccg ccgctgctgc cgcgtggccc tccgccagct gtttcgcgag   9000 

ctagtagagg ggcgccgctg ccgcgtacca gagccactgc cgcgccggca agagcgcccc   9060 

cgcgtcaacc ttctgcggcg ggcagtacag ggccaatacc caaccgcccc ccgacggtac   9120 

gccgtcccta tgccgcgatt gctacgtaga gttgttaaca acacatccat gaggcggcgg   9180 

ctccctggac tcgctcaggc gtagctggcc tagccttttg gagagctctt tccgcagatt   9240 

ggtcagtgtc agcgttccat ccgactcgtg gcaccgcccg ccgtcgcccg ccgccagccc   9300 

caacaaagac cgcctccacg acgactacta cattaatttc atccgccaga actctgccgc   9360 

ctaccagctg tcttcgtggt acaggaaccc aggccggacg acttacgcgt ccgccagccg   9420 

gtacggggtc cgaagcaaaa ctgtagccgc gtccagaaac atcatcagaa cgtactcgga   9480 

aagatggccg tgaagaagaa gaggaaggag aacaggacgt agagaacgta gatagcgacg   9540 

ccgccgccgc ctcaaaccgg catccaccgc gggagaagga gggtacgcac actggggctt   9600 

cggggagtag ccgacttcgt cccgatccag ccgctgttgc gcgagccgat tataccggac   9660 

gacgtggacg cactcccatc tgaccttcag taggtacagg tgtttcgcca ccatacgcgg   9720 

gcacaactac cacattcacg tcaaccggta ttgcctggtc aattgccaga ccactgggcc   9780 

gacgctctcg agccacatgg actctgcgct cattcgggag ctcagtttat gcatcagcaa   9840 

cgttcaggcg tggtccatga ccatagggtg gtttttcacg ccgccgccga ccgccatctc   9900 

cccggtcgca tcccaccggc cccgaggccc ccgctctaga aggttgtatt ccgctactat   9960 

aggcatctac atggacctgt aggtccacta cggccgccgc caccacctcc gcgcgccttt  10020 

cagcgcctgc gccaaggtct acaacgcgtc gccgtttttc acgaggtacc agccctgcga  10080 

gaccggccag tccgcgcgcg ttagcaactg cgagatctgg cacgttttcc tctcggacat  10140 

tcgcccgtga gaaggcacca gaccacctat ttaagcgttc ccatagtacc gcctgctggc  10200 

cccaagctcg gggcataggc cggcaggcgg cactaggtac gccaatggcg ggcgcacagc  10260 

ttgggtccac acgctgcagt ctgttgcccc ctcacgagga aaaccgaagg aaggtccgcg  10320 

ccgccgacga cgcgatcgaa aaaaccggtg accggcgcgc gtcgcattcg ccaatccgac  10380 

ctttcgcttt cgtaattcac cgagcgaggg acatcggcct cccaataaaa ggttcccaac  10440 

tcagcgccct gggggccaag ctcagagcct ggccggcctg acgccgcttg cccccaaacg  10500 

gaggggcagt acgttctggg gcgaacgttt aaggaggcct ttgtccctgc tcggggaaaa  10560 

aacgaaaagg gtctacgtag gccacgacgc cgtctacgcg gggggaggag tcgtcgccgt  10620 

tctcgttctc gtcgccgtct gtacgtcccg tgggagggga ggaggatggc gcagtcctcc  10680 

ccgctgtagg cgccaactgc gccgtcgtct accactaatg cttgggggcg ccgcggcccg  10740 

ggccgtgatg gacctgaacc tcctcccgct cccggaccgc gccgatcctc gcgggagagg  10800 

actcgccgtg ggttcccacg tcgacttcgc actatgcgca ctccgcatgc acggcgccgt  10860 

cttggacaaa gcgctggcgc tccctctcct cgggctcctc tacgccctag ctttcaaggt  10920 

gcgtcccgcg ctcgacgccg taccggactt agcgctcgcc aacgacgcgc tcctcctgaa  10980 

actcgggctg cgcgcttggc cctaatcagg gcgcgcgcgt gtgcaccgcc ggcggctgga  11040 

ccattggcgt atgctcgtct gccacttggt cctctaattg aaagtttttt cgaaattgtt  11100 

ggtgcacgca tgcgaacacc gcgcgctcct ccaccgatat cctgactacg tagacaccct  11160 

gaaacattcg cgcgacctcg ttttgggttt atcgttcggc gagtaccgcg tcgacaagga  11220 

atatcacgtc gtgtcgtccc tgttgctccg taagtcccta cgcgacgatt tgtatcatct  11280 

cgggctcccg gcgaccgacg agctaaacta tttgtaggac gtctcgtatc accacgtcct  11340 

cgcgtcgaac tcggaccgac tgttccaccg gcggtagttg ataaggtacg aatcggaccc  11400 

gttcaaaatg cgggcgttct atatggtatg gggaatgcaa gggtatctgt tcctccattt  11460 

ctagctcccc aagatgtacg cgtaccgcga cttccacgaa tggaactcgc tgctggaccc  11520 

gcaaatagcg ttgctcgcgt aggtgttccg gcactcgcac tcggccgccg cgctcgagtc  11580 

gctggcgctc gactacgtgt cggacgtttc ccgggaccga ccgtgcccgt cgccgctatc  11640 

tctccggctc aggatgaaac tgcgcccgcg actggacgcg acccggggtt cggctgcgcg  11700 

ggacctccgt cgaccccggc ctggacccga ccgccaccgt gggcgcgcgc gaccgttgca  11760 

gccgccgcac ctccttatac tgctcctgct actcatgctc ggtctcctgc cgctcatgat  11820 

tcgccactac aaagactagt ctactacgtt ctgcgttgcc tgggccgcca cgcccgccgc  11880 

gacgtctcgg tcggcaggcc ggaattgagg tgcctgctga ccgcggtcca gtacctggcg  11940 

tagtacagcg actgacgcgc gttaggactg cgcaaggccg tcgtcggcgt ccggttggcc  12000 

gagaggcgtt aagaccttcg ccaccagggc cgcgcgcgtt tggggtgcgt gctcttccac  12060 

gaccgctagc atttgcgcga ccggcttttg tcccggtagg ccgggctgct ccggccggac  12120 

cagatgctgc gcgacgaagt cgcgcaccga gcaatgttgt cgccgttgca cgtctggttg  12180 

gacctggccg accaccccct acacgcgctc cggcaccgcg tcgcactcgc gcgcgtcgtc  12240 

gtcccgttgg acccgaggta ccaacgtgat ttgcggaagg actcatgtgt cgggcggttg  12300 

cacggcgccc ctgtcctcct gatgtggttg aaacactcgc gtgacgccga ttaccactga  12360 

ctctgtggcg tttcactcca catggtcaga cccggtctga taaaaaaggt ctggtcatct  12420 

gttccggacg tctggcattt ggactcggtc cgaaagtttt tgaacgtccc cgacaccccc  12480 

cacgcccgag ggtgtccgct ggcgcgctgg cacagatcga acgactgcgg gttgagcgcg  12540 

gacaacgacg acgattatcg cgggaagtgc ctgtcaccgt cgcacagggc cctgtgtatg  12600 

gatccagtga acgactgtga catggcgctc cggtatccag tccgcgtaca cctgctcgta  12660 

tgaaaggtcc tctaatgttc acagtcggcg cgcgaccccg tcctcctgtg cccgtcggac  12720 

ctccgttggg atttgatgga cgactggttg gccgccgtct tctaggggag caacgtgtca  12780 

aatttgtcgc tcctcctcgc gtaaaacgcg atgcacgtcg tctcgcactc ggaattggac  12840 

tacgcgctgc cccattgcgg gtcgcaccgc gacctgtact ggcgcgcgtt gtaccttggc  12900 

ccgtacatac ggagtttggc cggcaaatag ttggcggatt acctgatgaa cgtagcgcgc  12960 

cggcggcact tggggctcat aaagtggtta cggtagaact tgggcgtgac cgatggcggg  13020 

ggaccaaaga tgtggccccc taagctccac gggctcccat tgctacctaa ggagaccctg  13080 

ctgtatctgc tgtcgcacaa aaggggcgtt ggcgtctggg acgatctcaa cgttgtcgcg  13140 

ctcgtccgtc tccgccgcga cgctttcctt tcgaaggcgt ccggttcgtc gaacaggcta  13200 

gatccgcgac gccggggcgc cagtctacga tcatcgggta aaggttcgaa ctatcccaga  13260 

gaatggtcgt gagcgtggtg ggcgggcgcg gacgacccgc tcctcctcat ggatttgttg  13320 

agcgacgacg tcggcgtcgc gctttttttg gacggaggcc gtaaagggtt gttgccctat  13380 

ctctcggatc acctgttcta ctcatctacc ttctgcatgc gcgtcctcgt gtccctgcac  13440 

ggtccgggcg cgggcgggtg ggcagcagtt tccgtgctgg cagtcgcccc agaccacacc  13500 

ctcctgctac tgagccgtct gctgtcgtcg caggacctaa accctccctc accgttgggc  13560 

aaacgcgtgg aagcggggtc cgacccctct tacaaaattt tttttttttt cgtactacgt  13620 

tttatttttt gagtggttcc ggtaccgtgg ctcgcaacca aaagaacata aggggaatca  13680 

tacgccgcgc gccgctacat actccttcca ggaggaggga ggatgctctc acaccactcg  13740 

cgccgcggtc accgccgccg cgacccaaga gggaagctac gaggggacct gggcggcaaa  13800 

cacggaggcg ccatggacgc cggatggccc ccctctttgt cgtaggcaat gagactcaac  13860 

cgtggggata agctgtggtg ggcacacatg gaccacctgt tgttcagttg cctacaccgt  13920 

agggacttga tggtcttgct ggtgtcgttg aaagactggt gccagtaagt tttgttactg  13980 

atgtcgggcc ccctccgttc gtgtgtctgg tagttagaac tgctggccag cgtgaccccg  14040 

ccgctggact tttggtagga cgtatggttg tacggtttac acttgctcaa gtacaaatgg  14100 

ttattcaaat tccgcgccca ctaccacagc gcgaacggat gattcctgtt agtccacctc  14160 

gactttatgc tcacccacct caagtgcgac gggctcccgt tgatgaggct ctggtactgg  14220 

tatctggaat acttgttgcg ctagcacctc gtgatgaact ttcacccgtc tgtcttgccc  14280 

caagaccttt cgctgtagcc ccatttcaaa ctgtgggcgt tgaagtctga ccccaaactg  14340 

gggcagtgac cagaacagta cggaccccat atatgtttgc ttcggaaggt aggtctgtag  14400 

taaaacgacg gtcctacgcc ccacctgaag tgggtgtcgg cggactcgtt gaacaacccg  14460 

taggcgttcg ccgttgggaa ggtcctcccg aaatcctagt ggatgctact agacctccca  14520 

ccattgtaag ggcgtgacaa cctacacctg cggatggtcc gctcgaactt tctactgtgg  14580 

cttgtcccgc ccccaccgcg tccgccgtcg ttgtcgtcac cgtcgccgcg ccttctcttg  14640 

aggttgcgcc gtcggcgccg ttacgtcggc cacctcctgt acttgctagt acggtaagcg  14700 

ccgctgtgga aacggtgtgc ccgactcctc ttcgcgcgac tccggcttcg tcgccggctt  14760 

cgacggcggg ggcgacgcgt tgggctccag ctcttcggag tcttctttgg ccactagttt  14820 

ggggactgtc tcctgtcgtt ctttgcgtca atgttggatt attcgttact gtcgtggaag  14880 

tgggtcatgg cgtcgaccat ggaacgtatg ttgatgccgc tgggagtctg gccttaggcg  14940 

agtacctggg acgaaacgtg aggactgcat tggacgccga gcctcgtcca gatgaccagc  15000 

aacggtctgt actacgttct ggggcactgg aaggcgaggt gcgcggtcta gtcgttgaaa  15060 

ggccaccacc cgcggctcga caacgggcac gtgaggttct cgaagatgtt gctggtccgg  15120 

cagatgaggg ttgagtaggc ggtcaaatgg agagactggg tgcacaagtt agcgaaaggg  15180 

ctcttggtct aaaaccgcgc gggcggtcgg gggtggtagt ggtggcagtc acttttgcaa  15240 

ggacgagagt gtctagtgcc ctgcgatggc gacgcgttgt cgtagcctcc tcaggtcgct  15300 

cactggtaat gactgcggtc tgcggcgtgg acggggatgc aaatgttccg ggacccgtat  15360 

cagagcggcg cgcaggatag ctcggcgtga aaaactcgtt cgtacaggta ggaatatagc  15420 

gggtcgttat tgtgtccgac cccggacgcg aagggttcgt tctacaaacc gccccggttc  15480 

ttcgcgaggc tggttgtggg tcacgcgcac gcgcccgtga tggcgcgcgg gaccccgcgc  15540 

gtgtttgcgc cggcgtgacc cgcgtggtgg cagctactgc ggtagctgcg ccaccacctc  15600 

ctccgcgcgt tgatgtgcgg gtgcggcggt ggtcacaggt gtcacctgcg ccggtaagtc  15660 

tggcaccacg cgcctcgggc cgcgatacga ttttacttct ctgccgcctc cgcgcatcgt  15720 

gcagcggtgg cggcggctgg gccgtgacgg cgggttgcgc gccgccgccg ggacgaattg  15780 

gcgcgtgcag cgtggccggc tgcccgccgg tacgcccggc gagcttccga ccggcgccca  15840 

taacagtgac acggggggtc caggtccgct gctcgccggc ggcgtcgtcg gcgccggtaa  15900 

tcacgatact gagtcccagc gtccccgttg cacataaccc acgcgctgag ccaatcgccg  15960 

gacgcgcacg ggcacgcgtg ggcggggggc gcgttgatct aacgttcttt tttgatgaat  16020 

ctgagcatga caacatacat aggtcgccgc cgccgcgcgt tgcttcgata caggttcgcg  16080 

ttttagtttc ttctctacga ggtccagtag cgcggcctct agataccggg gggcttcttc  16140 

cttctcgtcc taatgttcgg ggctttcgat ttcgcccagt ttttcttttt ctttctacta  16200 

ctactacttg aactgctgct ccaccttgac gacgtgcgat ggcgcgggtc cgctgcccat  16260 

gtcacctttc cagctgcgca ttttgcacaa aacgctgggc cgtggtggca tcagaaatgc  16320 

gggccactcg cgaggtgggc gtggatgttc gcgcacatac tactccacat gccgctgctc  16380 

ctggacgaac tcgtccggtt gctcgcggag cccctcaaac ggatgccttt cgccgtattc  16440 

ctgtacgacc gcaacggcga cctgctcccg ttgggttgtg gatcggattt cgggcattgt  16500 

gacgtcgtcc acgacgggcg cgaacgtggc aggcttcttt tcgcgccgga tttcgcgctc  16560 

agaccactga accgtgggtg gcacgtcgac taccatgggt tcgcggtcgc tgaccttcta  16620 

cagaaccttt tttactggca ccttggaccc gacctcgggc tccaggcgca cgccggttag  16680 

ttcgtccacc gcggccctga cccgcacgtc tggcacctgc aagtctatgg gtgatggtca  16740 

tcgtggtcat aacggtggcg gtgtctcccg tacctctgtg tttgcagggg ccaacggagt  16800 

cgccaccgcc tacggcgcca cgtccgccag cgacgccggc gcaggttctg gagatgcctc  16860 

cacgtttgcc tgggcaccta caaagcgcaa agtcgggggg ccgcgggcgc ggcaagctcc  16920 

ttcatgccgc ggcggtcgcg cgatgacggg cttatacggg atgtaggaag gtaacgcgga  16980 

tgggggccga tagcaccgat gtggatggcg gggtcttctg ctcgttgatg ggctgcggct  17040 

tggtggtgac cttgggcggc ggcggcagcg gcagcggtcg ggcacgaccg gggctaaagg  17100 

cacgcgtccc accgagcgct tcctccgtcc tgggaccacg acggttgtcg cgcgatggtg  17160 

gggtcgtagc aaattttcgg ccagaaacac caagaacgtc tataccggga gtggacggcg  17220 

gaggcaaagg gccacggccc taaggctcct tcttacgtgg catcctcccc gtaccggccg  17280 

gtgccggact gcccgccgta cgcagcacgc gtggtggccg ccgccgcgcg cagcgtggca  17340 

gcgtacgcgc cgccatagga cggggaggaa taaggtgact agcggcgccg ctaaccgcgg  17400 

cacgggcctt aacgtaggca ccggaacgtc cgcgtctctg tgactaattt ttgttcaacg  17460 

tacacctttt tagttttatt tttcagacct gagagtgcga gcgaaccagg acattgataa  17520 

aacatcttac cttctgtagt tgaaacgcag agaccggggc gctgtgccga gcgcgggcaa  17580 

gtaccctttg accgttctat agccgtggtc gttatactcg ccaccgcgga agtcgacccc  17640 

gagcgacacc tcgccgtaat ttttaaagcc aaggtggcaa ttcttgatac cgtcgttccg  17700 

gaccttgtcg tcgtgtccgg tctacgactc cctattcaac tttctcgttt taaaggttgt  17760 

tttccaccat ctaccggacc ggagaccgta atcgccccac cacctggacc ggttggtccg  17820 

tcacgtttta ttctaattgt cattcgaact aggggcggga gggcatctcc tcggaggtgg  17880 

ccggcacctc tgtcacagag gtctccccgc accgcttttc gcaggcgcgg ggctgtccct  17940 

tctttgagac cactgcgttt atctgctcgg agggagcatg ctcctccgtg atttcgttcc  18000 

ggacgggtgg tgggcagggt agcgcgggta ccgatggcct cacgacccgg tcgtgtgtgg  18060 

gcattgcgac ctggacggag gggggcggct gtgggtcgtc tttggacacg acggtccggg  18120 

ctggcggcaa caacattggg caggatcggc gcgcagggac gcggcgcggc ggtcgccagg  18180 

cgctagcaac gccgggcatc ggtcaccgtt gaccgtttcg tgtgacttgt cgtagcaccc  18240 

agacccccac gttagggact tcgcggctgc tacgaagact atcgattgca cagcatacac  18300 

acagtacata cgcaggtaca gcggcggtct cctcgacgac tcggcggcgc gcgggcgaaa  18360 

ggttctaccg atggggaagc tactacggcg tcaccagaat gtacgtgtag agcccggtcc  18420 

tgcggagcct catggactcg gggcccgacc acgtcaaacg ggcgcggtgg ctctgcatga  18480 

agtcggactt attgttcaaa tctttggggt gccaccgcgg atgcgtgctg cactggtgtc  18540 

tggccagggt cgcaaactgc gacgccaagt agggacacct ggcactccta tgacgcatga  18600 

gcatgttccg cgccaagtgg gatcgacacc cactattggc acacgacctg taccgaaggt  18660 

gcatgaaact gtaggcgccg cacgacctgt ccccgggatg aaaattcggg atgagaccgt  18720 

gacggatgtt gcgggaccga gggttcccac ggggtttagg aacgcttacc ctacttcgac  18780 

gatgacgaga actttatttg gatcttcttc tcctgctact gttgcttctg cttcatctgc  18840 

tcgttcgact cgtcgttttt tgagtgcata aacccgtccg cggaataaga ccatatttat  18900 

aatgtttcct cccataagtt tatccacagc ttccagtttg tggatttata cggctatttt  18960 

gtaaagttgg acttggagtt tatcctctta gagtcaccat gctttgtctt taattagtac  19020 

gtcgaccctc tcaggatttt ttctgatggg gttactttgg tacaatgcca agtatacgtt  19080 

ttgggtgttt acttttacct cccgttccgt aagaacattt cgttgtttta cctttcgatc  19140 

tttcagttca cctttacgtt aaaaagagtt gatgactccg tcggcgtccg ttaccactat  19200 

tgaactgagg atttcaccat aacatgtcac ttctacatct atatctttgg ggtctgtgag  19260 

tataaagaat gtacgggtga taattccttc cattgagtgc tcttgattac ccggttgtta  19320 

gatacgggtt gtccggatta atgtaacgaa aatccctgtt aaaataacca gattacataa  19380 

tgttgtcgtg cccattatac ccacaagacc gcccggttcg tagcgtcaac ttacgacaac  19440 

atctaaacgt tctgtctttg tgtctcgaaa gtatggtcga aaacgaacta aggtaaccac  19500 

tatcttggtc catgaaaaga tacaccttag tccgacaact gtcgatacta ggtctacaat  19560 

cttaataact tttagtacct tgacttctac ttgaaggttt aatgacgaaa ggtgaccctc  19620 

cacactaatt atgtctctga gaatggttcc attttggatt ttgtccagtc cttttaccta  19680 

ccctttttct acgatgtctt aaaagtctat ttttacttta ttctcaacct ttattaaaac  19740 

ggtaccttta gttagattta cggttggaca cctctttaaa ggacatgagg ttgtatcgcg  19800 

acataaacgg gctgttcgat ttcatgtcag gaaggttgca tttttaaaga ctattgggtt  19860 

tgtggatgct gatgtacttg ttcgctcacc accgagggcc cgatcacctg acgatgtaat  19920 

tggaacctcg tgcgaccagg gaactgatat acctgttgca gttgggtaaa ttggtggtgg  19980 

cgttacgacc ggacgcgatg gcgagttaca acgacccgtt accagcgata cacgggaagg  20040 

tgtaggtcca cggagtcttc aagaaacggt aatttttgga ggaagaggac ggcccgagta  20100 

tgtggatgct caccttgaag tccttcctac aattgtacca agacgtctcg agggatcctt  20160 

tactggattc ccaactgcct cggtcgtaat tcaaactatc gtaaacggaa atgcggtgga  20220 

agaaggggta ccgggtgttg tggcggaggt gcgaactccg gtacgaatct ttgctgtggt  20280 

tgctggtcag gaaattgctg atagagaggc ggcggttgta cgagatggga tatgggcggt  20340 

tgcgatggtt gcacgggtat aggtagggga gggcgttgac ccgccgaaag gcgccgaccc  20400 

ggaagtgcgc ggaattctga ttcctttggg gtagtgaccc gagcccgatg ctgggaataa  20460 

tgtggatgag accgagatat gggatggatc taccttggaa aatggagttg gtgtggaaat  20520 

tcttccaccg gtaatggaaa ctgagaagac agtcgaccgg accgttactg gcggacgaat  20580 

gggggttgct caaactttaa ttcgcgagtc aactgcccct cccaatgttg caacgggtca  20640 

cattgtactg gtttctgacc aaggaccatg tttacgatcg attgatattg taaccgatgg  20700 

tcccgaagat atagggtctc tcgatgttcc tggcgtacat gaggaagaaa tctttgaagg  20760 

tcgggtactc ggcagtccac cacctactat gatttatgtt cctgatggtt gtccacccgt  20820 

aggatgtggt tgtgttgttg agacctaaac aaccgatgga acgggggtgg tacgcgcttc  20880 

ctgtccggat gggacgattg aaggggatag gcgaatatcc gttctggcgt caactgtcgt  20940 

aatgggtctt tttcaaagaa acgctagcgt gggaaaccgc gtagggtaag aggtcattga  21000 

aatacaggta cccgcgtgag tgtctggacc cggttttgga agagatgcgg ttgaggcggg  21060 

tgcgcgatct gtactgaaaa ctccacctag ggtacctgct cgggtgggaa gaaatacaaa  21120 

acaaacttca gaaactgcac caggcacacg tggtcggcgt ggcgccgcag tagctttggc  21180 

acatggacgc gtgcgggaag agccggccgt tgcggtgttg tatttcttcg ttcgttgtag  21240 

ttgttgtcga cggcggtacc cgaggtcact cgtccttgac tttcggtaac agtttctaga  21300 

accaacaccc ggtataaaaa acccgtggat actgttcgcg aaaggtccga aacaaagagg  21360 

tgtgttcgag cggacgcggt atcagttatg ccggccagcg ctctgacccc cgcatgtgac  21420 

ctaccggaaa cggaccttgg gcgtgagttt ttgtacgatg gagaaactcg ggaaaccgaa  21480 

aagactggtc gctgagttcg tccaaatggt caaactcatg ctcagtgagg acgcggcatc  21540 

gcggtaacga agaagggggc tggcgacata ttgcgacctt ttcaggtggg tttcgcatgt  21600 

ccccgggttg agccggcgga cacctgataa gacgacgtac aaagaggtgc ggaaacggtt  21660 

gaccggggtt tgagggtacc tagtgttggg gtggtacttg gaataatggc cccatgggtt  21720 

gaggtacgag ttgtcagggg tccatgtcgg gtgggacgca gcgttggtcc ttgtcgagat  21780 

gtcgaaggac ctcgcggtga gcgggatgaa ggcgtcggtg tcacgcgtct aatcctcgcg  21840 

gtgaagaaaa acagtgaact ttttgtacat ttttattaca tgatctctgt gaaagttatt  21900 

tccgtttacg aaaataaaca tgtgagagcc cactaataaa tgggggtggg aacggcagac  21960 

gcggcaaatt tttagtttcc ccaagacggc gcgtagcgat acgcggtgac cgtccctgtg  22020 

caacgctatg accacaaatc acgaggtgaa tttgagtccg tgttggtagg cgccgtcgag  22080 

ccacttcaaa agtgaggtgt ccgacgcgtg gtagtggttg cgcaaatcgt ccagcccgcg  22140 

gctatagaac ttcagcgtca accccggagg cgggacgcgc gcgctcaacg ctatgtgtcc  22200 

caacgtcgtg accttgtgat agtcgcggcc caccacgtgc gaccggtcgt gcgagaacag  22260 

cctctagtct aggcgcaggt ccaggaggcg caacgagtcc cgcttgcctc agttgaaacc  22320 

atcgacggaa gggtttttcc cgcgcacggg tccgaaactc aacgtgagcg tggcatcacc  22380 

gtagttttcc actggcacgg gccagacccg caatcctatg tcgcggacgt attttcggaa  22440 

ctagacgaat tttcggtgga ctcggaaacg cggaagtctc ttcttgtacg gcgttctgaa  22500 

cggccttttg actaaccggc ctgtccggcg cagcacgtgc gtcgtggaac gcagccacaa  22560 

cctctagacg tggtgtaaag ccggggtggc caagaagtgc tagaaccgga acgatctgac  22620 

gaggaagtcg cgcgcgacgg gcaaaagcga gcagtgtagg taaagttagt gcacgaggaa  22680 

taaatagtat tacgaaggca catctgtgaa ttcgagcgga agctagagtc gcgtcgccac  22740 

gtcggtgttg cgcgtcgggc acccgagcac tacgaacatc cagtggagac gtttgctgac  22800 

gtccatgcgg acgtccttag cggggtagta gcagtgtttc cagaacaacg accacttcca  22860 

gtcgacgttg ggcgccacga ggagcaagtc ggtccagaac gtatgccggc ggtctcgaag  22920 

gtgaaccagt ccgtcatcaa acttcaagcg gaaatctagc aataggtgca ccatgaacag  22980 

gtagtcgcgc gcgcgtcgga ggtacgggaa gagggtgcgt ctgtgctagc cgtgtgagtc  23040 

gcccaagtag tggcattaaa gtgaaaggcg aagcgacccg agaaggagaa ggagaacgca  23100 

ggcgtatggt gcgcggtgac ccagcagaag taagtcggcg gcgtgacacg cgaatggagg  23160 

aaacggtacg aactaatcgt ggccacccaa cgactttggg tggtaaacat cgcggtgtag  23220 

aagagaaaga aggagcgaca ggtgctaatg gagaccacta ccgcccgcga gcccgaaccc  23280 

tcttcccgcg aagaaaaaga agaacccgcg ttaccggttt aggcggcggc tccagctacc  23340 

ggcgcccgac ccacacgcgc cgtggtcgcg cagaacacta ctcagaagga gcaggagcct  23400 

gagctatgcg gcggagtagg cgaaaaaacc cccgcgggcc cctccgccgc cgctgcccct  23460 

gcccctgctg tgcaggaggt accaaccccc tgcagcgcgg cgtggcgcag gcgcgagccc  23520 

ccaccaaagc gcgacgagga gaagggctga ccggtaaagg aagaggatat ccgtcttttt  23580 

ctagtacctc agtcagctct tcttcctgtc ggattggcgg gggagactca agcggtggtg  23640 

gcggaggtgg ctacggcggt tgcgcggatg gtggaagggg cagctccgtg ggggcgaact  23700 

cctcctcctt cactaatagc tcgtcctggg tccaaaacat tcgcttctgc tgctcctggc  23760 

gagtcatggt tgtctcctat ttttcgttct ggtcctgttg cgtctccgtt tgctccttgt  23820 

tcagcccgcc cccctgcttt ccgtaccgct gatggatcta caccctctgc tgcacgacaa  23880 

cttcgtagac gtcgcggtca cgcggtaata gacgctgcgc aacgttctcg cgtcgctaca  23940 

cggggagcgg tatcgcctac agtcggaacg gatgcttgcg gtggataaga gtggcgcgca  24000 

tggggggttt gcggttcttt tgccgtgtac gctcgggttg ggcgcggagt tgaagatggg  24060 

gcataaacgg cacggtctcc acgaacggtg gatagtgtag aaaaaggttt tgacgttcta  24120 

tggggatagg acggcacggt tggcgtcggc tcgcctgttc gtcgaccgga acgccgtccc  24180 

gcgacagtat ggactatagc ggagcgagtt gcttcacggt ttttagaaac tcccagaacc  24240 

tgcgctgctc ttcgcgcgcc gtttgcgaga cgttgtcctt ttgtcgcttt tactttcagt  24300 

gagacctcac aaccaccttg agctcccact gttgcgcgcg gatcggcatg attttgcgtc  24360 

gtagctccag tgggtgaaac ggatgggccg tgaattggat ggggggttcc agtactcgtg  24420 

tcagtactca ctcgactagc acgcggcacg cgtcggggac ctctccctac gtttaaacgt  24480 

tcttgtttgt ctcctcccgg atgggcgtca accgctgctc gtcgatcgcg cgaccgaagt  24540 

ttgcgcgctc ggacggctga acctcctcgc tgcgtttgat tactaccggc gtcacgagca  24600 

atggcacctc gaactcacgt acgtcgccaa gaaacgactg ggcctctacg tcgcgttcga  24660 

tctcctttgt aacgtgatgt ggaaagctgt cccgatgcat gcggtccgga cgttctagag  24720 

gttgcacctc gagacgttgg accagaggat ggaaccttaa aacgtgcttt tggcggaacc  24780 

cgttttgcac gaagtaaggt gcgagttccc gctccgcgcg gcgctgatgc aggcgctgac  24840 

gcaaatgaat aaagatacga tgtggaccgt ctgccggtac ccgcaaaccg tcgtcacgaa  24900 

cctcctcacg ttggagttcc tcgacgtctt tgacgatttc gttttgaact tcctggatac  24960 

ctgccggaag ttgctcgcga ggcaccggcg cgtggaccgc ctgtagtaaa aggggcttgc  25020 

ggacgaattt tgggacgttg tcccagacgg tctgaagtgg tcagtttcgt acaacgtctt  25080 

gaaatccttg aaataggatc tcgcgagtcc ttagaacggg cggtggacga cacgtgaagg  25140 

atcgctgaaa cacgggtaat tcatggcgct tacgggaggc ggcgaaaccc cggtgacgat  25200 

ggaagacgtc gatcggttga tggaacggat ggtgagactg tattaccttc tgcactcgcc  25260 

actgccagat gacctcacag tgacagcgac gttggatacg tggggcgtgg cgagggacca  25320 

aacgttaagc gtcgacgaat tgctttcagt ttaatagcca tggaaactcg acgtcccagg  25380 

gagcggactg cttttcaggc gccgaggccc caactttgag tgaggccccg acacctgcag  25440 

ccgaatggaa gcgtttaaac atggactcct gatggtgcgg gtgctctaat ccaagatgct  25500 

tctggttagg gcgggcggat tacgcctcga atggcggacg cagtaatggg tcccggtgta  25560 

agaaccggtt aacgttcggt agttgtttcg ggcggttctc aaagacgatg ctttccctgc  25620 

cccccaaatg aacctggggg tcaggccgct cctcgagttg ggttaggggg gcggcggcgt  25680 

cgggatagtc gtcgtcggcg cccgggaacg aagggtccta ccgtgggttt ttcttcgacg  25740 

tcgacggcgg cggtgggtgc ctgctcctcc ttatgaccct gtcagtccgt ctcctccaaa  25800 

acctgctcct cctcctcctg tactaccttc tgaccctctc ggatctgctc cttcgaaggc  25860 

tccagcttct ccacagtctg ctttgtggca gtgggagcca gcgtaagggg agcggccgcg  25920 

gggtctttag ccgttggcca aggtcgtacc gatgttggag gcgaggagtc cgcggcggcc  25980 

gtgacgggca agcggctggg ttggcatcta ccctgtggtg accttggtcc cggccattca  26040 

ggttcgtcgg cggcggcaat cgggttctcg ttgttgtcgc ggttccgatg gcgagtaccg  26100 

cgcccgtgtt cttgcggtat caacgaacga acgttctgac acccccgttg tagaggaagc  26160 

gggcggcgaa agaagagatg gtagtgccgc accggaaggg ggcattgtag gacgtaatga  26220 

tggcagtaga gatgtcgggt atgacgtggc cgccgtcgcc gtcgttgtcg tcgccggtgt  26280 

gtcttcgttt ccgctggcct atcgttctga gactgtttcg ggttctttag gtgtcgccgc  26340 

cgtcgtcgtc ctcctcctcg cgacgcagac cgcgggttgc ttgggcatag ctgggcgctc  26400 

gaatctttgt cctaaaaagg gtgagacata cgatataaag ttgtctcgtc cccggttctt  26460 

gttctcgact tttatttttt gtccagagac gctagggagt gggcgtcgac ggacatagtg  26520 

ttttcgcttc tagtcgaagc cgcgtgcgac cttctgcgcc tccgagagaa gtcatttatg  26580 

acgcgcgact gagaattcct gatcaaagcg cgggaaagag tttaaattcg cgcttttgat  26640 

gcagtagagg tcgccggtgt gggccgcggt cgtggacaac agtcgcggta atactcgttc  26700 

ctttaagggt gcgggatgta cacctcaatg gtcggtgttt accctgaacg ccgacctcga  26760 

cgggttctga tgagttgggc ttatttgatg tactcgcgcc ctggggtgta ctatagggcc  26820 

cagttgcctt atgcgcgggt ggctttggct taagaggacc ttgtccgccg ataatggtgg  26880 

tgtggagcat tattggaatt aggggcatca accgggcgac gggaccacat ggtcctttca  26940 

gggcgagggt ggtgacacca tgaagggtct ctgcgggtcc ggcttcaagt ctactgattg  27000 

agtccccgcg tcgaacgccc gccgaaagca gtgtcccacg ccagcgggcc cgtcccatat  27060 

tgagtggact gttagtctcc cgctccataa gtcgagttgc tgctcagcca ctcgaggagc  27120 

gaaccagagg caggcctgcc ctgtaaagtc tagccgccgc ggccggcgag aagtaagtgc  27180 

ggagcagtcc gttaggattg agacgtctgg agcaggagac tcggcgcgag acctccgtaa  27240 

ccttgagacg ttaaataact cctcaaacac ggtagccaga tgaaattggg gaagagccct  27300 

ggagggccgg tgataggcct agttaaataa ggattgaaac tgcgccattt cctgagccgc  27360 

ctgccgatgc tgacttacaa ttcacctctc cgtctcgttg acgcggactt tgtggaccag  27420 

gtgacagcgg cggtgttcac gaaacgggcg ctgaggccac tcaaaacgat gaaacttaac  27480 

gggctcctag tatagctccc gggccgcgtg ccgcaggccg aatggcgggt ccctctcgaa  27540 

cgggcatcgg actaagccct caaatgggtc gcgggggacg atcaactcgc cctgtcccct  27600 

gggacacaag agtgacacta aacgttgaca ggattgggac ctaatgtagt tctagaaaca  27660 

acggtagaga cacgactcat attatttatg tctttaattt tatatgaccc cgaggatagc  27720 

ggtaggacat ttgcggtggc agaagtgggc gggttcgttt ggttccgctt ggaatggacc  27780 

atgaaaattg tagagaggga gacactaaat gttgtcaaag ttgggtctgc ctcactcaga  27840 

tgctctcttg gagaggctcg agtcgatgag gtagtctttt ttgtggtggg aggaatggac  27900 

ggcccttgca tgctcacgca gtggccggcg acgtggtgtg gatggcggac tggcatttgg  27960 

tctgaaaaag gcctgtctgg agttattgag acaaatggtc ttgtcctcca ctcgaatctt  28020 

ttgggaatcc cataatccgg tttccgcgtc gatgacaccc caaatacttg ttaagttcgt  28080 

tgagatgccc gataagatta agtccaaaga gatcttagcc ccaaccccaa taagagacag  28140 

aacactaaga gaaataagaa tatgattgcg aagagacgga ttccgagcgg cggacgacac  28200 

acgtgtaaac gtaaataaca gtcgaaaaat ttgcgacccc agcggtgggt tctactaatc  28260 

catgtattag gatccaaatg agtgggaacg cagtcgggtg ccatggtggg ttttccacct  28320 

aaaattcctc ggtcggacat tacaatgtaa gcgtcgactt cgattactca cgtggtgaga  28380 

atattttacg tggtgtcttg tacttttcga cgaataagcg gtgtttttgt tttaaccgtt  28440 

catacgacaa atacgataaa ccgtcggtcc actgtgatgt ctcatattac aatgtcaaaa  28500 

ggtcccattt tcagtatttt gaaaatacat atgaaaaggt aaaatacttt acacgctgta  28560 

atggtacatg tactcgtttg tcatattcaa caccgggggt gttttaacac accttttgtg  28620 

accgtgaaag acgacgtgac gatacgatta atgtcacgag cgaaaccaga catgggatga  28680 

gatataattt atgttttcgt ctgcgtcgaa ataactcctt ttcttttacg gaattaaatg  28740 

attcaatgtt tcgattacag tggtgattga cgaaatgagc gacgaacgtt ttgtttaagt  28800 

ttttcaatcg taatattaat cttatcctaa atttgggggg ccagtaaagg acgagttatg  28860 

gtaaggggac ttgttaactg agatacaccc tatacgaggt cgcgatgttg gaacttcagt  28920 

ccgaaggacc tacagtcgta gactgaaacc ggtcgtggac agggcgccta aacaaggtca  28980 

ggttgatgtc gctgggtggg attgtctcta ctggttgtgt tggttgcgcc ggcggcgatg  29040 

gcctgaatgt agatggtgtt tatgtggggt tcaaagacgg aaacagttat tgaccctatt  29100 

gaacccgtac accaccaaga ggtatcgcga atacaaacat acggaataat aatacaccga  29160 

gtagacgacg gatttcgcgt ttgcgcgggc tggtgggtag atatcagggt agtaacacga  29220 

tgtgggtttg ttactacctt aggtatctaa cctgcctgac tttgtgtaca agaaaagaga  29280 

atgtcatact aatttactct gtactaagga gctcaaaaat ataatgactg ggaacaacgc  29340 

gaaaaaacac gcacgaggtg taaccgacgc caaagagtgt agcttcatct gacgtaaggt  29400 

cggaagtgtc agataaacga aatgcctaaa cagtgggagt gcgagtagac gtcggagtag  29460 

tgacaccagt agcggaaata ggtcacgtaa ctgacccaga cacacgcgaa acgtatagag  29520 

tctgtggtag gggtcatgtc cctgtcctga tatcgactcg aagaatctta agaaattaat  29580 

actttaaatg acactgaaaa gacgactaat aaacgtggga tagacgcaaa acaaggggct  29640 

ggaggttcgg agtttctgta tatagtacgt ctaagtgagc atatacctta taaggttcaa  29700 

cgatgttact tttttcgcta gaaaggcttc ggaccaatat acgttagtag agacaatacc  29760 

acaagacgtc atggtagaat cgggatcgat atatagggat ggaactgtaa ccgaccttgc  29820 

gttatctacg gtacttggtg ggttgaaagg ggcgcgggcg atacgaaggt gacgttgttc  29880 

aacaacggcc gccgaaacag ggtcggttag tcggagcggg tggaagaggg tgggggtgac  29940 

tttagtcgat gaaattagat tgtcctcctc tactgactgt gggatctaga tctttacctg  30000 

ccttaataat gtctcgtcgc ggacgatctt tctgcgtccc gtcgccggct cgttgtcgcg  30060 

tacttagttc tcgaggttct gtaccaattg aacgtggtca cgttttcccc atagaaaaca  30120 

gagcatttcg tccggtttca gtggatgctg tcattatggt ggcctgtggc ggaatcgatg  30180 

ttcaacggtt ggttcgcagt ctttaaccac cagtaccacc ctcttttcgg gtaatggtat  30240 

tgagtcgtga gccatctttg gcttccgacg taagtgagtg gaacagttcc tggactccta  30300 

gagacgtggg aataattctg ggacacgcca gagtttctag aataagggaa attgattatt  30360 

tttttttatt atttcgtagt gaatgaattt tagtcaatcg tttaaagaca ggtcaaataa  30420 

gtcgtcgtgg aggaacggga ggagggtcga gaccataacg tcgaaggagg accgacgttt  30480 

gaaagaggtg ttagatttac cttacagtca aaggaggaca aggacaggta ggcgtgggtg  30540 

atagaagtac aacaacgtct acttcgcgcg ttctggcaga cttctatgga agttggggca  30600 

cataggtata ctgtgccttt ggccaggagg ttgacacgga aaagaatgag gagggaaaca  30660 

tagggggtta cccaaagttc tctcaggggg accccatgag agaaacgcgg ataggcttgg  30720 

agatcaatgg aggttaccgt acgaacgcga gttttacccg ttgccggaga gagacctgct  30780 

ccggccgttg gaatggaggg ttttacattg gtgacactcg ggtggagagt ttttttggtt  30840 

cagtttgtat ttggaccttt atagacgtgg ggagtgtcaa tggagtcttc gggattgaca  30900 

ccgacggcgg cgtggagatt accagcgccc gttgtgtgag tggtacgtta gtgtccgggg  30960 

cgattggcac gtgctgaggt ttgaatcgta acggtgggtt cctggggagt gtcacagtct  31020 

tcctttcgat cgggacgttt gtagtccggg ggagtggtgg tggctatcgt catgggaatg  31080 

atagtgacgg agtgggggaa attgatgacg gtgaccatcg aacccgtaac tgaactttct  31140 

cgggtaaata tgtgttttac cttttgatcc tgatttcatg ccccgaggaa acgtacattg  31200 

tctgctggat ttgtgaaact ggcatcgttg accaggtcca cactgataat tattatgaag  31260 

gaacgtttga tttcaatgac ctcggaaccc aaaactaagt gttccgttat acgttgaatt  31320 

acatcgtcct cctgattcct aactaagagt tttgtctgcg gaatatgaac tacaatcaat  31380 

aggcaaacta cgagttttgg ttgatttaga ttctgatcct gtcccgggag aaaaatattt  31440 

gagtcgggtg ttgaacctat aattgatgtt gtttccggaa atgaacaaat gtcgaagttt  31500 

gttaaggttt ttcgaactcc aattggattc gtgacggttc cccaactaca aactgcgatg  31560 

tcggtatcgg taattacgtc ctctacccga acttaaacca agtggattac gtggtttgtg  31620 

tttaggggag ttttgttttt aaccggtacc ggatcttaaa ctaagtttgt tccgatacca  31680 

aggatttgat ccttgaccgg aatcaaaact gtcgtgtcca cggtaatgtc atcctttgtt  31740 

tttattacta ttcgattgaa acacctggtg tggtcgaggt agaggattga catctgattt  31800 

acgtctcttt ctacgatttg agtgaaacca gaattgtttt acaccgtcag tttatgaacg  31860 

atgtcaaagt caaaaccgac aatttccgtc aaaccgaggt tatagacctt gtcaagtttc  31920 

acgagtagaa taatattcta aactgctttt acctcacgat gatttgttaa ggaaggacct  31980 

gggtcttata accttgaaat ctttacctct agaatgactt ccgtgtcgga tatgtttgcg  32040 

acaacctaaa tacggattgg atagtcgaat aggttttaga gtgccatttt gacggttttc  32100 

attgtaacag tcagttcaaa tgaatttgcc tctgttttga tttggacatt gtgattggta  32160 

atgtgatttg ccatgtgtcc tttgtcctct gtgttgaggt tcacgtatga gatacagtaa  32220 

aagtaccctg accagaccgg tgttgatgta attactttat aaacggtgta ggagaatgtg  32280 

aaaaagtatg taacgggttc ttatttctta gcaaacacaa tacaaagttg cacaaataaa  32340 

aagttaacgt cttttaaagt tcagtaaaaa gtaagtcatc atatcggggt ggtggtgtat  32400 

cgaatatgtc tagtggcatg gaattagttt gagtgtcttg ggatcataag ttggacggtg  32460 

gagggagggt tgtgtgtctc atgtgtcagg aaagaggggc cgaccggaat ttttcgtagt  32520 

atagtaccca ttgtctgtat aagaatccac aatataaggt gtgccaaagg acagctcggt  32580 

ttgcgagtag tcactataat tatttgaggg gcccgtcgag tgaattcaag tacagcgaca  32640 

ggtcgacgac tcggtgtccg acgacaggtt gaacgccaac gaattgcccg ccgcttcctc  32700 

ttcaggtgcg gatgtacccc catctcagta ttagcacgta gtcctatccc gccaccacga  32760 

cgtcgtcgcg cgcttatttg acgacggcgg cggcgaggca ggacgtcctt atgttgtacc  32820 

gtcaccagag gagtcgctac taagcgtggc gggcgtcgta ttccgcggaa caggaggccc  32880 

gtgtcgtcgc gtgggactag agtgaattta gtcgtgtcat tgacgtcgtg tcgtggtgtt  32940 

ataacaagtt ttagggtgtc acgttccgcg acataggttt cgagtaccgc ccctggtgtc  33000 

ttgggtgcac cggtagtatg gtgttcgcgt ccatctaatt caccgctggg gagtatttgt  33060 

gcgacctgta tttgtaatgg agaaaaccgt acaacattaa gtggtggagg gccatggtat  33120 

atttggagac taatttgtac cgcggtaggt ggtggtagga tttggtcgac cggttttgga  33180 

cgggcggccg atatgtgacg tcccttggcc ctgaccttgt tactgtcacc tctcgggtcc  33240 

tgagcattgg tacctagtag tacgagcagt actatagtta caaccgtgtt gtgtccgtgt  33300 

gcacgtatgt gaaggagtcc taatgttcga ggagggcgca atcttggtat agggtccctt  33360 

gttgggtaag gacttagtcg catttagggt gtgacgtccc ttctggagcg tgcattgagt  33420 

gcaacacgta acagtttcac aatgtaagcc cgtcgtcgcc tactaggagg tcataccatc  33480 

gcgcccaaag acagagtttt cctccatctg ctagggatga catgcctcac gcggctctgt  33540 

tggctctagc acaaccagca tcacagtacg gtttaccttg cggcctgcat cagtataaag  33600 

gacttcgttt tggtccacgc ccgcactgtt tgtctagacg cagaggccag agcggcgaat  33660 

ctagcgagac acatcatcaa catcatatag gtgagagagt ttcgtaggtc cgcgggggac  33720 

cgaagcccaa gatacatttg aggaagtacg cggcgacggg actattgtag gtggtggcgt  33780 

cttattcggt gtgggtcggt tggatgtgta agcaagacgc tcagtgtgtg ccctcctcgc  33840 

ccttctcgac cttcttggta caaaaaaaaa aataaggttt tctaataggt tttggagttt  33900 

tacttctaga taattcactt gcgcgagggg aggccaccgc accagtttga gatgtcggtt  33960 

tcttgtctat taccgtaaac attctacaac gtgttaccga aggttttccg tttgccggga  34020 

gtgcaggttc acctgcattt ccgatttggg aagtcccact tagaggagat atttgtaagg  34080 

tcgtggaagt tggtacgggt ttattaagag tagagcggtg gaagagttat atagagattc  34140 

gtttagggct tataattcag gccggtaaca tttttagacg aggtctcgcg ggaggtggaa  34200 

gtcggagttc gtcgcttagt actaacgttt ttaagtccaa ggagtgtctg gacatattct  34260 

aagttttcgc cttgtaattg tttttatggc gctagggcat ccagggaagc gtcccggtcg  34320 

acttgtatta gcacgtccag acgtgcctgg tcgcgccggt gaaggggcgg tccttggtac  34380 

tgttttcttg ggtgtgacta atactgtgcg tatgagcctc gatacgattg gtcgcatcgg  34440 

ggctacattc gaacaacgta cccgccgcta tattttacgt tccacgacga gttttttagt  34500 

ccgtttcgga gcgcgttttt tctttcgtgt agcatcagta cgagtacgtc tatttccgtc  34560 

cattcgaggc cttggtggtg tctttttctg tggtaaaaag agagtttgta cagacgccca  34620 

aagacgtatt tgtgttttat tttattgttt ttttgtaaat ttgtaatctt cggacagaat  34680 

gttgtccttt ttgttgggaa tattcgtatt ctgcctgatg ccggtacggc cgcactggca  34740 

tttttttgac cagtggcact aatttttcgt ggtggctgtc gaggagccag tacaggcctc  34800 

agtattacat tctgagccat ttgtgtagtc caactaagtg tagccagtca cgatttttcg  34860 

ctggctttat cgggccccct tatgtatggg cgtccgcatc tctgttgtaa tgtcgggggt  34920 

atcctccata ttgttttaat tatcctctct ttttgtgtat ttgtggactt tttgggagga  34980 

cggatccgtt ttatcgtggg agggcgaggt cttgttgtat gtcgcgaagg tgtcgccgtc  35040 

ggtattgtca gtcggaatgg tcattttttc ttttggataa tttttttgtg gtgagctgtg  35100 

ccgtggtcga gttagtcagt gtcacatttt ttcccggttc acgtctcgct catatatatc  35160 

ctgatttttt actgcattgc caatttcagg tgttttttgt gggtcttttg gcgtgcgctt  35220 

ggatgcgggt ctttgctttc ggttttttgg gtgttgaagg agtttagcag tgaaggcaaa  35280 

agggtgcaat gcagtgaagg gtaaaattct tttgatgtta agggttgtgt atgttcaatg  35340 

aggcgggatt ttggatgcag tgggcggggc aagggtgcgg ggcgcggtgc agtgtttgag  35400 

gtgggggagt aatagtataa ccgaagttag gttttattcc atataataac tactacaatt  35460 

aattcttaag cctagacgct gcgctccgac ctaccggaag gggtaatact aagaagagcg  35520 

aaggccgccg tagccctacg ggcgcaacgt ccggtacgac aggtccgtcc atctactgct  35580 

ggtagtccct gtcgaagttc cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga  35640 

ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg cgagttcagt  35700 

ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac cttcgaggga  35760 

gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga aagagggaag  35820 

cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc acatccagca  35880 

agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga cgcggaatag  35940 

gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg  36000 

gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca agaacttcac  36060 

caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag acgacttcgg  36120 

tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt ggcgaccatc  36180 

gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta gagttcttct  36240 

aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg caattcccta  36300 

aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttag ttagatttca  36360 

tatatactca tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt  36420 

cgctagacag ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc  36480 

tatgccctcc cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt  36540 

ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca  36600 

ggacgttgaa ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca  36660 

tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt  36720 

gcgagcagca aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt  36780 

actagggggt acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct  36840 

tcattcaacc ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga  36900 

cagtacggta ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact  36960 

cttatcacat acgccgctgg ctcaacgaga acgggccgca gttgtgccct attatggcgc  37020 

ggtgtatcgt cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag  37080 

agttcctaga atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact  37140 

agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta  37200 

cggcgttttt tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa  37260 

gttataataa cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca  37320 

taaatctttt tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg  37380 

cagattcttt ggtaataata gtactgtaat tggatatttt tatccgcata gtgctccggg  37440 

aaagcagaag ttcttaacct aggcttaaga atta                              37474 

 
           
             8  
             10  
             DNA  
             Artificial Sequence  
             
               adenovirus serotype 34  
             
           
            8 

gtgagtccta                                                            10 

 
           
             9  
             11  
             DNA  
             Artificial Sequence  
             
               adenovirus serotype 34  
             
           
            9 

ccgcactttc t                                                          11 

 
           
             10  
             11  
             DNA  
             Artificial Sequence  
             
               adenovirus serotype 34  
             
           
            10 

attgacattg g                                                          11 

 
           
             11  
             12  
             DNA  
             Artificial Sequence  
             
               adenovirus serotype 34  
             
           
            11 

cagtctggag ga                                                         12 

 
           
             12  
             6  
             DNA  
             Artificial Sequence  
             
               adenovirus serotype 34  
             
           
            12 

ggagga                                                                 6 

 
           
             13  
             34796  
             DNA  
             adenovirus serotype 35  
           
            13 

catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtgatttta     60 

aaaagtgtgg gccgtgtggt gattggctgt ggggttaacg gttaaaaggg gcggcgcggc    120 

cgtgggaaaa tgacgtttta tgggggtgga gtttttttgc aagttgtcgc gggaaatgtt    180 

acgcataaaa aggcttcttt tctcacggaa ctacttagtt ttcccacggt atttaacagg    240 

aaatgaggta gttttgaccg gatgcaagtg aaaattgctg attttcgcgc gaaaactgaa    300 

tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg    360 

ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt    420 

ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt    480 

tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc    540 

tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat    600 

aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga    660 

cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt    720 

agaggtagag ggatcggagg attctaatga ggaagctgtg aatggctttt ttaccgattc    780 

tatgctttta gctgctaatg aaggattaga attagatccg cctttggaca ctttcaatac    840 

tccaggggtg attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt    900 

ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga    960 

aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt   1020 

tcagttggat tgcccggagc ttctggacat ggctgtaagt cttgtgaatt tcacaggaaa   1080 

aatactggag taaaggaact gttatgttcg cttttgttat atgaaaaccc actgccactt   1140 

tatttacagt aaagtgtgtt taagttaaaa tttaaaggaa tatgctgttt ttcacatgta   1200 

tattgagtgt gagttttgtg cttcttatta taagtcctgt gtctgatgct gatgaatcac   1260 

catctcctga ttctactacc tcacctcctg atattcaagc acctgttcct gtggacgtgc   1320 

gcaagcccat tcctgtgaag cttaagcctg ggaaacgtcc agcagtggag aaacttgagg   1380 

acttgttaca gggtggggac ggacctttgg acttgagtac acggaaacgt ccaagacaat   1440 

aagtgttcca tatccgtgtt tacttaaggt gacgtcaata tttgtgtgag agtgcaatgt   1500 

aataaaaata tgttaactgt tcactggttt ttattgcttt ttgggcgggg actcaggtat   1560 

ataagtagaa gcagacctgt gtggttagct cataggagct ggctttcatc catggaggtt   1620 

tgggccattt tggaagacct taggaagact aggcaactgt tagagagcgc ttcggacgga   1680 

gtctccggtt tttggagatt ctggttcgct agtgaattag ctagggtagt ttttaggata   1740 

aaacaggact ataaacaaga atttgaaaag ttgttggtag attgcccagg actttttgaa   1800 

gctcttaatt tgggccatca ggttcacttt aaagaaaaag ttttatcagt tttagacttt   1860 

tcaaccccag gtagaactgc tgctgctgtg gcttttctta cttttatatt agataaatgg   1920 

atcccgcaga ctcatttcag caggggatac gttttggatt tcatagccac agcattgtgg   1980 

agaacatgga aggttcgcaa gatgaggaca atcttaggtt actggccagt gcagcctttg   2040 

ggtgtagcgg gaatcctgag gcatccaccg gtcatgccag cggttctgga ggaggaacag   2100 

caagaggaca acccgagagc cggcctggac cctccagtgg aggaggcgga gtagctgact   2160 

tgtctcctga actgcaacgg gtgcttactg gatctacgtc cactggacgg gataggggcg   2220 

ttaagaggga gagggcatcc agtggtactg atgctagatc tgagttggct ttaagtttaa   2280 

tgagtcgcag acgtcctgaa accatttggt ggcatgaggt tcagaaagag ggaagggatg   2340 

aagtttctgt attgcaggag aaatattcac tggaacaggt gaaaacatgt tggttggagc   2400 

cagaggatga ttgggaggtg gccattaaaa attatgccaa gatagctttg aggcctgata   2460 

aacagtataa gatcagtaga cggattaata tccggaatgc ttgttacata tctggaaatg   2520 

gggctgaggt ggtaatagat actcaagaca agacagttat tagatgctgc atgatggata   2580 

tgtggcctgg agtagtcggt atggaagcag tcacttttgt aaatgttaag tttaggggag   2640 

atggttataa tggaatagtg tttatggcca ataccaaact tatattgcat ggttgtagct   2700 

tttttggttt caacaatacc tgtgtagatg cctggggaca ggttagtgta cgggggtgta   2760 

gtttctatgc gtgttggatt gccacagctg gcagaaccaa gagtcaattg tctctgaaga   2820 

aatgcatatt ccaaagatgt aacctgggca ttctgaatga aggcgaagca agggtccgtc   2880 

actgcgcttc tacagatact ggatgtttta ttttaattaa gggaaatgcc agcgtaaagc   2940 

ataacatgat ttgtggtgct tccgatgaga ggccttatca aatgctcact tgtgctggtg   3000 

ggcattgtaa tatgctggct actgtgcata ttgtttccca tcaacgcaaa aaatggcctg   3060 

tttttgatca caatgtgttg accaagtgca ccatgcatgc aggtgggcgt agaggaatgt   3120 

ttatgcctta ccagtgtaac atgaatcatg tgaaagtgtt gttggaacca gatgcctttt   3180 

ccagaatgag cctaacagga atctttgaca tgaacacgca aatctggaag atcctgaggt   3240 

atgatgatac gagatcgagg gtgcgcgcat gcgaatgcgg aggcaagcat gccaggttcc   3300 

agccggtgtg tgtagatgtg accgaagatc tcagaccgga tcatttggtt attgcccgca   3360 

ctggagcaga gttcggatcc agtggagaag aaactgacta aggtgagtat tgggaaaact   3420 

ttggggtggg attttcagat ggacagattg agtaaaaatt tgttttttct gtcttgcagc   3480 

tgacatgagt ggaaatgctt cttttaaggg gggagtcttc agcccttatc tgacagggcg   3540 

tctcccatcc tgggcaggag ttcgtcagaa tgttatggga tctactgtgg atggaagacc   3600 

cgttcaaccc gccaattctt caacgctgac ctatgctact ttaagttctt cacctttgga   3660 

cgcagctgca gccgctgccg ccgcctctgt cgccgctaac actgtgcttg gaatgggtta   3720 

ctatggaagc atcgtggcta attccacttc ctctaataac ccttctacac tgactcagga   3780 

caagttactt gtccttttgg cccagctgga ggctttgacc caacgtctgg gtgaactttc   3840 

tcagcaggtg gccgagttgc gagtacaaac tgagtctgct gtcggcacgg caaagtctaa   3900 

ataaaaaaaa ttccagaatc aatgaataaa taaacgagct tgttgttgat ttaaaatcaa   3960 

gtgtttttat ttcatttttc gcgcacggta tgccctggac caccgatctc gatcattgag   4020 

aactcggtgg attttttcca gaatcctata gaggtgggat tgaatgttta gatacatggg   4080 

cattaggccg tctttggggt ggagatagct ccattgaagg gattcatgct ccggggtagt   4140 

gttgtaaatc acccagtcat aacaaggtcg cagtgcatgg tgttgcacaa tatcttttag   4200 

aagtaggctg attgccacag ataagccctt ggtgtaggtg tttacaaacc ggttgagctg   4260 

ggaggggtgc attcgaggtg aaattatgtg cattttggat tggattttta agttggcaat   4320 

attgccgcca agatcccgtc ttgggttcat gttatgaagg actaccaaga cggtgtatcc   4380 

ggtacattta ggaaatttat cgtgcagctt ggatggaaaa gcgtggaaaa atttggagac   4440 

acccttgtgt cctccgagat tttccatgca ctcatccatg ataatagcaa tggggccgtg   4500 

ggcagcggcg cgggcaaaca cgttccgtgg gtctgacaca tcatagttat gttcctgagt   4560 

taaatcatca taagccattt taatgaattt ggggcggagc gtaccagatt ggggtatgaa   4620 

tgttccttcg ggccccggag catagttccc ctcacagatt tgcatttccc aagctttcag   4680 

ttctgagggt ggaatcatgt ccacctgggg ggctatgaag aacaccgttt cgggggcggg   4740 

ggtgattagt tgggatgata gcaagtttct gagcaattga gatttgccac atccggtggg   4800 

gccataaata attccgatta caggttgcag gtggtagttt agggaacggc aactgccgtc   4860 

ttctcgaagc aagggggcca cctcgttcat catttccctt acatgcatat tttcccgcac   4920 

caaatccatt aggaggcgct ctcctcctag tgatagaagt tcttgtagtg aggaaaagtt   4980 

tttcagcggt tttagaccgt cagccatggg cattttggaa agagtttgct gcaaaagttc   5040 

tagtctgttc cacagttcag tgatgtgttc tatggcatct cgatccagca gacctcctcg   5100 

tttcgcgggt ttggacggct cctggagtag ggtatgagac gatgggcgtc cagcgctgcc   5160 

agggttcggt ccttccaggg tctcagtgtt cgagtcaggg ttgtttccgt cacagtgaag   5220 

gggtgtgcgc ctgcttgggc gcttgccagg gtgcgcttca gactcattct gctggtggag   5280 

aacttctgtc gcttggcgcc ctgtatgtcg gccaagtagc agtttaccat gagttcgtag   5340 

ttgagcgcct cggctgcgtg gcctttggcg cggagcttac ctttggaagt tttcttgcat   5400 

accgggcagt ataggcattt cagcgcatac agcttgggcg caaggaaaat ggattctggg   5460 

gagtatgcat ccgcgccgca ggaggcgcaa acagtttcac attccaccag ccaggttaaa   5520 

tccggttcat tggggtcaaa aacaagtttt ccgccatatt ttttgatgcg tttcttacct   5580 

ttggtctcca taagttcgtg tcctcgttga gtgacaaaca ggctgtccgt atctccgtag   5640 

actgatttta caggcctctt ctccagtgga gtgcctcggt cttcttcgta caggaactct   5700 

gaccactctg atacaaaggc gcgcgtccag gccagcacaa aggaggctat gtgggagggg   5760 

tagcgatcgt tgtcaaccag ggggtccacc ttttccaaag tatgcaaaca catgtcaccc   5820 

tcttcaacat ccaggaatgt gattggcttg taggtgtatt tcacgtgacc tggggtcccc   5880 

gctggggggg tataaaaggg ggcggttctt tgctcttcct cactgtcttc cggatcgctg   5940 

tccaggaacg tcagctgttg gggtaggtat tccctctcga aggcgggcat gacctctgca   6000 

ctcaggttgt cagtttctaa gaacgaggag gatttgatat tgacagtgcc ggttgagatg   6060 

cctttcatga ggttttcgtc catttggtca gaaaacacaa tttttttatt gtcaagtttg   6120 

gtggcaaatg atccatacag ggcgttggat aaaagtttgg caatggatcg catggtttgg   6180 

ttcttttcct tgtccgcgcg ctctttggcg gcgatgttga gttggacata ctcgcgtgcc   6240 

aggcacttcc attcggggaa gatagttgtt aattcatctg gcacgattct cacttgccac   6300 

cctcgattat gcaaggtaat taaatccaca ctggtggcca cctcgcctcg aaggggttca   6360 

ttggtccaac agagcctacc tcctttccta gaacagaaag ggggaagtgg gtctagcata   6420 

agttcatcgg gagggtctgc atccatggta aagattcccg gaagtaaatc cttatcaaaa   6480 

tagctgatgg gagtggggtc atctaaggcc atttgccatt ctcgagctgc cagtgcgcgc   6540 

tcatatgggt taaggggact gccccagggc atgggatggg tgagagcaga ggcatacatg   6600 

ccacagatgt catagacgta gatgggatcc tcaaagatgc ctatgtaggt tggatagcat   6660 

cgcccccctc tgatacttgc tcgcacatag tcatatagtt catgtgatgg cgctagcagc   6720 

cccggaccca agttggtgcg attgggtttt tctgttctgt agacgatctg gcgaaagatg   6780 

gcgtgagaat tggaagagat ggtgggtctt tgaaaaatgt tgaaatgggc atgaggtaga   6840 

cctacagagt ctctgacaaa gtgggcataa gattcttgaa gcttggttac cagttcggcg   6900 

gtgacaagta cgtctagggc gcagtagtca agtgtttctt gaatgatgtc ataacctggt   6960 

tggtttttct tttcccacag ttcgcggttg agaaggtatt cttcgcgatc cttccagtac   7020 

tcttctagcg gaaacccgtc tttgtctgca cggtaagatc ctagcatgta gaactgatta   7080 

actgccttgt aagggcagca gcccttctct acgggtagag agtatgcttg agcagctttt   7140 

cgtagcgaag cgtgagtaag ggcaaaggtg tctctgacca tgactttgag aaattggtat   7200 

ttgaagtcca tgtcgtcaca ggctccctgt tcccagagtt ggaagtctac ccgtttcttg   7260 

taggcggggt tgggcaaagc gaaagtaaca tcattgaaga gaatcttacc ggctctgggc   7320 

ataaaattgc gagtgatgcg gaaaggctgt ggtacttccg ctcgattgtt gatcacctgg   7380 

gcagctagga cgatttcgtc gaaaccgttg atgttgtgtc ctacgatgta taattctatg   7440 

aaacgcggcg tgcctctgac gtgaggtagc ttactgagct catcaaaggt taggtctgtg   7500 

gggtcagata aggcgtagtg ttcgagagcc cattcgtgca ggtgaggatt tgcatgtagg   7560 

aatgatgacc aaagatctac cgccagtgct gtttgtaact ggtcccgata ctgacgaaaa   7620 

tgccggccaa ttgccatttt ttctggagtg acacagtaga aggttctggg gtcttgttgc   7680 

catcgatccc acttgagttt aatggctaga tcgtgggcca tgttgacgag acgctcttct   7740 

cctgagagtt tcatgaccag catgaaagga actagttgtt tgccaaagga tcccatccag   7800 

gtgtaagttt ccacatcgta ggtcaggaag agtctttctg tgcgaggatg agagccgatc   7860 

gggaagaact ggatttcctg ccaccagttg gaggattggc tgttgatgtg atggaagtag   7920 

aagtttctgc ggcgcgccga gcattcgtgt ttgtgcttgt acagacggcc gcagtagtcg   7980 

cagcgttgca cgggttgtat ctcgtgaatg agctgtacct ggcttccctt gacgagaaat   8040 

ttcagtggga agccgaggcc tggcgattgt atctcgtgct cttctatatt cgctgtatcg   8100 

gcctgttcat cttctgtttc gatggtggtc atgctgacga gcccccgcgg gaggcaagtc   8160 

cagacctcgg cgcgggaggg gcggagctga aggacgagag cgcgcaggct ggagctgtcc   8220 

agagtcctga gacgctgcgg actcaggtta gtaggtaggg acagaagatt aacttgcatg   8280 

atcttttcca gggcgtgcgg gaggttcaga tggtacttga tttccacagg ttcgtttgta   8340 

gagacgtcaa tggcttgcag ggttccgtgt cctttgggcg ccactaccgt acctttgttt   8400 

tttcttttga tcggtggtgg ctctcttgct tcttgcatgc tcagaagcgg tgacggggac   8460 

gcgcgccggg cggcagcggt tgttccggac ccgggggcat ggctggtagt ggcacgtcgg   8520 

cgccgcgcac gggcaggttc tggtattgcg ctctgagaag acttgcgtgc gccaccacgc   8580 

gtcgattgac gtcttgtatc tgacgtctct gggtgaaagc taccggcccc gtgagcttga   8640 

acctgaaaga gagttcaaca gaatcaattt cggtatcgtt aacggcagct tgtctcagta   8700 

tttcttgtac gtcaccagag ttgtcctggt aggcgatctc cgccatgaac tgctcgattt   8760 

cttcctcctg aagatctccg cgacccgctc tttcgacggt ggccgcgagg tcattggaga   8820 

tacggcccat gagttgggag aatgcattca tgcccgcctc gttccagacg cggctgtaaa   8880 

ccacggcccc ctcggagtct cttgcgcgca tcaccacctg agcgaggtta agctccacgt   8940 

gtctggtgaa gaccgcatag ttgcataggc gctgaaaaag gtagttgagt gtggtggcaa   9000 

tgtgttcggc gacgaagaaa tacatgatcc atcgtctcag cggcatttcg ctaacatcgc   9060 

ccagagcttc caagcgctcc atggcctcgt agaagtccac ggcaaaatta aaaaactggg   9120 

agtttcgcgc ggacacggtc aattcctcct cgagaagacg gatgagttcg gctatggtgg   9180 

cccgtacttc gcgttcgaag gctcccggga tctcttcttc ctcttctatc tcttcttcca   9240 

ctaacatctc ttcttcgtct tcaggcgggg gcggaggggg cacgcggcga cgtcgacggc   9300 

gcacgggcaa acggtcgatg aatcgttcaa tgacctctcc gcggcggcgg cgcatggttt   9360 

cagtgacggc gcggccgttc tcgcgcggtc gcagagtaaa aacaccgccg cgcatctcct   9420 

taaagtggtg actgggaggt tctccgtttg ggagggagag ggcgctgatt atacatttta   9480 

ttaattggcc cgtagggact gcgcgcagag atctgatcgt gtcaagatcc acgggatctg   9540 

aaaacctttc gacgaaagcg tctaaccagt cacagtcaca aggtaggctg agtacggctt   9600 

cttgtgggcg ggggtggtta tgtgttcggt ctgggtcttc tgtttcttct tcatctcggg   9660 

aaggtgagac gatgctgctg gtgatgaaat taaagtaggc agttctaaga cggcggatgg   9720 

tggcgaggag caccaggtct ttgggtccgg cttgctggat acgcaggcga ttggccattc   9780 

cccaagcatt atcctgacat ctagcaagat ctttgtagta gtcttgcatg agccgttcta   9840 

cgggcacttc ttcctcaccc gttctgccat gcatacgtgt gagtccaaat ccgcgcattg   9900 

gttgtaccag tgccaagtca gctacgactc tttcggcgag gatggcttgc tgtacttggg   9960 

taagggtggc ttgaaagtca tcaaaatcca caaagcggtg gtaagcccct gtattaatgg  10020 

tgtaagcaca gttggccatg actgaccagt taactgtctg gtgaccaggg cgcacgagct  10080 

cggtgtattt aaggcgcgaa taggcgcggg tgtcaaagat gtaatcgttg caggtgcgca  10140 

ccagatactg gtaccctata agaaaatgcg gcggtggttg gcggtagaga ggccatcgtt  10200 

ctgtagctgg agcgccaggg gcgaggtctt ccaacataag gcggtgatag ccgtagatgt  10260 

acctggacat ccaggtgatt cctgcggcgg tagtagaagc ccgaggaaac tcgcgtacgc  10320 

ggttccaaat gttgcgtagc ggcatgaagt agttcattgt aggcacggtt tgaccagtga  10380 

ggcgcgcgca gtcattgatg ctctatagac acggagaaaa tgaaagcgtt cagcgactcg  10440 

actccgtagc ctggaggaac gtgaacgggt tgggtcgcgg tgtaccccgg ttcgagactt  10500 

gtactcgagc cggccggagc cgcggctaac gtggtattgg cactcccgtc tcgacccagc  10560 

ctacaaaaat ccaggatacg gaatcgagtc gttttgctgg tttccgaatg gcagggaagt  10620 

gagtcctatt tttttttttt ttttgccgct cagatgcatc ccgtgctgcg acagatgcgc  10680 

ccccaacaac agcccccctc gcagcagcag cagcagcagc aaccacaaaa ggctgtccct  10740 

gcaactactg caactgccgc cgtgagcggt gcgggacagc ccgcctatga tctggacttg  10800 

gaagagggcg aaggactggc acgtctaggt gcgccttcgc ccgagcggca tccgcgagtt  10860 

caactgaaaa aagattctcg cgaggcgtat gtgccccaac agaacctatt tagagacaga  10920 

agcggcgagg agccggagga gatgcgagct tcccgcttta acgcgggtcg tgagctgcgt  10980 

cacggtttgg accgaagacg agtgttgcga gacgaggatt tcgaagttga tgaagtgaca  11040 

gggatcagtc ctgccagggc acacgtggct gcagccaacc ttgtatcggc ttacgagcag  11100 

acagtaaagg aagagcgtaa cttccaaaag tcttttaata atcatgtgcg aaccctgatt  11160 

gcccgcgaag aagttaccct tggtttgatg catttgtggg atttgatgga agctatcatt  11220 

cagaacccta ctagcaaacc tctgaccgcc cagctgtttc tggtggtgca acacagcaga  11280 

gacaatgagg ctttcagaga ggcgctgctg aacatcaccg aacccgaggg gagatggttg  11340 

tatgatctta tcaacattct acagagtatc atagtgcagg agcggagcct gggcctggcc  11400 

gagaaggtag ctgccatcaa ttactcggtt ttgagcttgg gaaaatatta cgctcgcaaa  11460 

atctacaaga ctccatacgt tcccatagac aaggaggtga agatagatgg gttctacatg  11520 

cgcatgacgc tcaaggtctt gaccctgagc gatgatcttg gggtgtatcg caatgacaga  11580 

atgcatcgcg cggttagcgc cagcaggagg cgcgagttaa gcgacaggga actgatgcac  11640 

agtttgcaaa gagctctgac tggagctgga accgagggtg agaattactt cgacatggga  11700 

gctgacttgc agtggcagcc tagtcgcagg gctctgagcg ccgcgacggc aggatgtgag  11760 

cttccttaca tagaagaggc ggatgaaggc gaggaggaag agggcgagta cttggaagac  11820 

tgatggcaca acccgtgttt tttgctagat ggaacagcaa gcaccggatc ccgcaatgcg  11880 

ggcggcgctg cagagccagc cgtccggcat taactcctcg gacgattgga cccaggccat  11940 

gcaacgtatc atggcgttga cgactcgcaa ccccgaagcc tttagacagc aaccccaggc  12000 

caaccgtcta tcggccatca tggaagctgt agtgccttcc cgatctaatc ccactcatga  12060 

gaaggtcctg gccatcgtga acgcgttggt ggagaacaaa gctattcgtc cagatgaggc  12120 

cggactggta tacaacgctc tcttagaacg cgtggctcgc tacaacagta gcaatgtgca  12180 

aaccaatttg gaccgtatga taacagatgt acgcgaagcc gtgtctcagc gcgaaaggtt  12240 

ccagcgtgat gccaacctgg gttcgctggt ggcgttaaat gctttcttga gtactcagcc  12300 

tgctaatgtg ccgcgtggtc aacaggatta tactaacttt ttaagtgctt tgagactgat  12360 

ggtatcagaa gtacctcaga gcgaagtgta tcagtccggt cctgattact tctttcagac  12420 

tagcagacag ggcttgcaga cggtaaatct gagccaagct tttaaaaacc ttaaaggttt  12480 

gtggggagtg catgccccgg taggagaaag agcaaccgtg tctagcttgt taactccgaa  12540 

ctcccgcctg ttattactgt tggtagctcc tttcaccgac agcggtagca tcgaccgtaa  12600 

ttcctatttg ggttacctac taaacctgta tcgcgaagcc atagggcaaa gtcaggtgga  12660 

cgagcagacc tatcaagaaa ttacccaagt cagtcgcgct ttgggacagg aagacactgg  12720 

cagtttggaa gccactctga acttcttgct taccaatcgg tctcaaaaga tccctcctca  12780 

atatgctctt actgcggagg aggagaggat ccttagatat gtgcagcaga gcgtgggatt  12840 

gtttctgatg caagaggggg caactccgac tgcagcactg gacatgacag cgcgaaatat  12900 

ggagcccagc atgtatgcca gtaaccgacc tttcattaac aaactgctgg actacttgca  12960 

cagagctgcc gctatgaact ctgattattt caccaatgcc atcttaaacc cgcactggct  13020 

gcccccacct ggtttctaca cgggcgaata tgacatgccc gaccctaatg acggatttct  13080 

gtgggacgac gtggacagcg atgttttttc acctctttct gatcatcgca cgtggaaaaa  13140 

ggaaggcggt gatagaatgc attcttctgc atcgctgtcc ggggtcatgg gtgctaccgc  13200 

ggctgagccc gagtctgcaa gtccttttcc tagtctaccc ttttctctac acagtgtacg  13260 

tagcagcgaa gtgggtagaa taagtcgccc gagtttaatg ggcgaagagg agtacctaaa  13320 

cgattccttg ctcagaccgg caagagaaaa aaatttccca aacaatggaa tagaaagttt  13380 

ggtggataaa atgagtagat ggaagactta tgctcaggat cacagagacg agcctgggat  13440 

catggggact acaagtagag cgagccgtag acgccagcgc catgacagac agaggggtct  13500 

tgtgtgggac gatgaggatt cggccgatga tagcagcgtg ttggacttgg gtgggagagg  13560 

aaggggcaac ccgtttgctc atttgcgccc tcgcttgggt ggtatgttgt gaaaaaaaat  13620 

aaaaaagaaa aactcaccaa ggccatggcg acgagcgtac gttcgttctt ctttattatc  13680 

tgtgtctagt ataatgaggc gagtcgtgct aggcggagcg gtggtgtatc cggagggtcc  13740 

tcctccttcg tacgagagcg tgatgcagca gcagcaggcg acggcggtga tgcaatcccc  13800 

actggaggct ccctttgtgc ctccgcgata cctggcacct acggagggca gaaacagcat  13860 

tcgttactcg gaactggcac ctcagtacga taccaccagg ttgtatctgg tggacaacaa  13920 

gtcggcggac attgcttctc tgaactatca gaatgaccac agcaacttct tgaccacggt  13980 

ggtgcagaac aatgacttta cccctacgga agccagcacc cagaccatta actttgatga  14040 

acgatcgcgg tggggcggtc agctaaagac catcatgcat actaacatgc caaacgtgaa  14100 

cgagtatatg tttagtaaca agttcaaagc gcgtgtgatg gtgtccagaa aacctcccga  14160 

cggtgctgca gttggggata cttatgatca caagcaggat attttggaat atgagtggtt  14220 

cgagtttact ttgccagaag gcaacttttc agttactatg actattgatt tgatgaacaa  14280 

tgccatcata gataattact tgaaagtggg tagacagaat ggagtgcttg aaagtgacat  14340 

tggtgttaag ttcgacacca ggaacttcaa gctgggatgg gatcccgaaa ccaagttgat  14400 

catgcctgga gtgtatacgt atgaagcctt ccatcctgac attgtcttac tgcctggctg  14460 

cggagtggat tttaccgaga gtcgtttgag caaccttctt ggtatcagaa aaaaacagcc  14520 

atttcaagag ggttttaaga ttttgtatga agatttagaa ggtggtaata ttccggccct  14580 

cttggatgta gatgcctatg agaacagtaa gaaagaacaa aaagccaaaa tagaagctgc  14640 

tacagctgct gcagaagcta aggcaaacat agttgccagc gactctacaa gggttgctaa  14700 

cgctggagag gtcagaggag acaattttgc gccaacacct gttccgactg cagaatcatt  14760 

attggccgat gtgtctgatg gaacggacgt gaaactcact attcaacctg tagaaaaaga  14820 

tagtaagaat agaagctata atgtgttgga agacaaaatc aacacagcct atcgcagttg  14880 

gtatctttcg tacaattatg gcgatcccga aaaaggagtg cgttcctgga cattgctcac  14940 

cacctcagat gtcacctgcg gagcagagca ggtttactgg tcgcttccag acatgatgaa  15000 

ggatcctgtc actttccgct ccactagaca agtcagtaac taccctgtgg tgggtgcaga  15060 

gcttatgccc gtcttctcaa agagcttcta caacgaacaa gctgtgtact cccagcagct  15120 

ccgccagtcc acctcgctta cgcacgtctt caaccgcttt cctgagaacc agattttaat  15180 

ccgtccgccg gcgcccacca ttaccaccgt cagtgaaaac gttcctgctc tcacagatca  15240 

cgggaccctg ccgttgcgca gcagtatccg gggagtccaa cgtgtgaccg ttactgacgc  15300 

cagacgccgc acctgtccct acgtgtacaa ggcactgggc atagtcgcac cgcgcgtcct  15360 

ttcaagccgc actttctaaa aaaaaaatgt ccattcttat ctcgcccagt aataacaccg  15420 

gttggggtct gcgcgctcca agcaagatgt acggaggcgc acgcaaacgt tctacccaac  15480 

atcccgtgcg tgttcgcgga cattttcgcg ctccatgggg tgccctcaag ggccgcactc  15540 

gcgttcgaac caccgtcgat gatgtaatcg atcaggtggt tgccgacgcc cgtaattata  15600 

ctcctactgc gcctacatct actgtggatg cagttattga cagtgtagtg gctgacgctc  15660 

gcaactatgc tcgacgtaag agccggcgaa ggcgcattgc cagacgccac cgagctacca  15720 

ctgccatgcg agccgcaaga gctctgctac gaagagctag acgcgtgggg cgaagagcca  15780 

tgcttagggc ggccagacgt gcagcttcgg gcgccagcgc cggcaggtcc cgcaggcaag  15840 

cagccgctgt cgcagcggcg actattgccg acatggccca atcgcgaaga ggcaatgtat  15900 

actgggtgcg tgacgctgcc accggtcaac gtgtacccgt gcgcacccgt ccccctcgca  15960 

cttagaagat actgagcagt ctccgatgtt gtgtcccagc ggcgaggatg tccaagcgca  16020 

aatacaagga agaaatgctg caggttatcg cacctgaagt ctacggccaa ccgttgaagg  16080 

atgaaaaaaa accccgcaaa atcaagcggg ttaaaaagga caaaaaagaa gaggaagatg  16140 

gcgatgatgg gctggcggag tttgtgcgcg agtttgcccc acggcgacgc gtgcaatggc  16200 

gtgggcgcaa agttcgacat gtgttgagac ctggaacttc ggtggtcttt acacccggcg  16260 

agcgttcaag cgctactttt aagcgttcct atgatgaggt gtacggggat gatgatattc  16320 

ttgagcaggc ggctgaccga ttaggcgagt ttgcttatgg caagcgtagt agaataactt  16380 

ccaaggatga gacagtgtca atacccttgg atcatggaaa tcccacccct agtcttaaac  16440 

cggtcacttt gcagcaagtg ttacccgtaa ctccgcgaac aggtgttaaa cgcgaaggtg  16500 

aagatttgta tcccactatg caactgatgg tacccaaacg ccagaagttg gaggacgttt  16560 

tggagaaagt aaaagtggat ccagatattc aacctgaggt taaagtgaga cccattaagc  16620 

aggtagcgcc tggtctgggg gtacaaactg tagacattaa gattcccact gaaagtatgg  16680 

aagtgcaaac tgaacccgca aagcctactg ccacctccac tgaagtgcaa acggatccat  16740 

ggatgcccat gcctattaca actgacgccg ccggtcccac tcgaagatcc cgacgaaagt  16800 

acggtccagc aagtctgttg atgcccaatt atgttgtaca cccatctatt attcctactc  16860 

ctggttaccg aggcactcgc tactatcgca gccgaaacag tacctcccgc cgtcgccgca  16920 

agacacctgc aaatcgcagt cgtcgccgta gacgcacaag caaaccgact cccggcgccc  16980 

tggtgcggca agtgtaccgc aatggtagtg cggaaccttt gacactgccg cgtgcgcgtt  17040 

accatccgag tatcatcact taatcaatgt tgccgctgcc tccttgcaga tatggccctc  17100 

acttgtcgcc ttcgcgttcc catcactggt taccgaggaa gaaactcgcg ccgtagaaga  17160 

gggatgttgg gacgcggaat gcgacgctac aggcgacggc gtgctatccg caagcaattg  17220 

cggggtggtt ttttaccagc cttaattcca attatcgctg ctgcaattgg cgcgatacca  17280 

ggcatagctt ccgtggcggt tcaggcctcg caacgacatt gacattggaa aaaaaacgta  17340 

taaataaaaa aaaatacaat ggactctgac actcctggtc ctgtgactat gttttcttag  17400 

agatggaaga catcaatttt tcatccttgg ctccgcgaca cggcacgaag ccgtacatgg  17460 

gcacctggag cgacatcggc acgagccaac tgaacggggg cgccttcaat tggagcagta  17520 

tctggagcgg gcttaaaaat tttggctcaa ccataaaaac atacgggaac aaagcttgga  17580 

acagcagtac aggacaggcg cttagaaata aacttaaaga ccagaacttc caacaaaaag  17640 

tagtcgatgg gatagcttcc ggcatcaatg gagtggtaga tttggctaac caggctgtgc  17700 

agaaaaagat aaacagtcgt ttggacccgc cgccagcaac cccaggtgaa atgcaagtgg  17760 

aggaagaaat tcctccgcca gaaaaacgag gcgacaagcg tccgcgtccc gatttggaag  17820 

agacgctggt gacgcgcgta gatgaaccgc cttcttatga ggaagcaacg aagcttggaa  17880 

tgcccaccac tagaccgata gccccaatgg ccaccggggt gatgaaacct tctcagttgc  17940 

atcgacccgt caccttggat ttgccccctc cccctgctgc tactgctgta cccgcttcta  18000 

agcctgtcgc tgccccgaaa ccagtcgccg tagccaggtc acgtcccggg ggcgctcctc  18060 

gtccaaatgc gcactggcaa aatactctga acagcatcgt gggtctaggc gtgcaaagtg  18120 

taaaacgccg tcgctgcttt taattaaata tggagtagcg cttaacttgc ctatctgtgt  18180 

atatgtgtca ttacacgccg tcacagcagc agaggaaaaa aggaagaggt cgtgcgtcga  18240 

cgctgagtta ctttcaagat ggccacccca tcgatgctgc cccaatgggc atacatgcac  18300 

atcgccggac aggatgcttc ggagtacctg agtccgggtc tggtgcagtt cgcccgcgcc  18360 

acagacacct acttcaatct gggaaataag tttagaaatc ccaccgtagc gccgacccac  18420 

gatgtgacca ccgaccgtag ccagcggctc atgttgcgct tcgtgcccgt tgaccgggag  18480 

gacaatacat actcttacaa agtgcggtac accctggccg tgggcgacaa cagagtgctg  18540 

gatatggcca gcacgttctt tgacattagg ggcgtgttgg acagaggtcc cagtttcaaa  18600 

ccctattctg gtacggctta caactctctg gctcctaaag gcgctccaaa tgcatctcaa  18660 

tggattgcaa aaggcgtacc aactgcagca gccgcaggca atggtgaaga agaacatgaa  18720 

acagaggaga aaactgctac ttacactttt gccaatgctc ctgtaaaagc cgaggctcaa  18780 

attacaaaag agggcttacc aataggtttg gagatttcag ctgaaaacga atctaaaccc  18840 

atctatgcag ataaacttta tcagccagaa cctcaagtgg gagatgaaac ttggactgac  18900 

ctagacggaa aaaccgaaga gtatggaggc agggctctaa agcctactac taacatgaaa  18960 

ccctgttacg ggtcctatgc gaagcctact aatttaaaag gtggtcaggc aaaaccgaaa  19020 

aactcggaac cgtcgagtga aaaaattgaa tatgatattg acatggaatt ttttgataac  19080 

tcatcgcaaa gaacaaactt cagtcctaaa attgtcatgt atgcagaaaa tgtaggtttg  19140 

gaaacgccag acactcatgt agtgtacaaa cctggaacag aagacacaag ttccgaagct  19200 

aatttgggac aacagtctat gcccaacaga cccaactaca ttggcttcag agataacttt  19260 

attggactca tgtactataa cagtactggt aacatggggg tgctggctgg tcaagcgtct  19320 

cagttaaatg cagtggttga cttgcaggac agaaacacag aactttctta ccaactcttg  19380 

cttgactctc tgggcgacag aaccagatac tttagcatgt ggaatcaggc tgtggacagt  19440 

tatgatcctg atgtacgtgt tattgaaaat catggtgtgg aagatgaact tcccaactat  19500 

tgttttccac tggacggcat aggtgttcca acaaccagtt acaaatcaat agttccaaat  19560 

ggagaagata ataataattg gaaagaacct gaagtaaatg gaacaagtga gatcggacag  19620 

ggtaatttgt ttgccatgga aattaacctt caagccaatc tatggcgaag tttcctttat  19680 

tccaatgtgg ctctgtatct cccagactcg tacaaataca ccccgtccaa tgtcactctt  19740 

ccagaaaaca aaaacaccta cgactacatg aacgggcggg tggtgccgcc atctctagta  19800 

gacacctatg tgaacattgg tgccaggtgg tctctggatg ccatggacaa tgtcaaccca  19860 

ttcaaccacc accgtaacgc tggcttgcgt taccgatcta tgcttctggg taacggacgt  19920 

tatgtgcctt tccacataca agtgcctcaa aaattcttcg ctgttaaaaa cctgctgctt  19980 

ctcccaggct cctacactta tgagtggaac tttaggaagg atgtgaacat ggttctacag  20040 

agttccctcg gtaacgacct gcgggtagat ggcgccagca tcagtttcac gagcatcaac  20100 

ctctatgcta cttttttccc catggctcac aacaccgctt ccacccttga agccatgctg  20160 

cggaatgaca ccaatgatca gtcattcaac gactacctat ctgcagctaa catgctctac  20220 

cccattcctg ccaatgcaac caatattccc atttccattc cttctcgcaa ctgggcggct  20280 

ttcagaggct ggtcatttac cagactgaaa accaaagaaa ctccctcttt ggggtctgga  20340 

tttgacccct actttgtcta ttctggttct attccctacc tggatggtac cttctacctg  20400 

aaccacactt ttaagaaggt ttccatcatg tttgactctt cagtgagctg gcctggaaat  20460 

gacaggttac tatctcctaa cgaatttgaa ataaagcgca ctgtggatgg cgaaggctac  20520 

aacgtagccc aatgcaacat gaccaaagac tggttcttgg tacagatgct cgccaactac  20580 

aacatcggct atcagggctt ctacattcca gaaggataca aagatcgcat gtattcattt  20640 

ttcagaaact tccagcccat gagcaggcag gtggttgatg aggtcaatta caaagacttc  20700 

aaggccgtcg ccatacccta ccaacacaac aactctggct ttgtgggtta catggctccg  20760 

accatgcgcc aaggtcaacc ctatcccgct aactatccct atccactcat tggaacaact  20820 

gccgtaaata gtgttacgca gaaaaagttc ttgtgtgaca gaaccatgtg gcgcataccg  20880 

ttctcgagca acttcatgtc tatgggggcc cttacagact tgggacagaa tatgctctat  20940 

gccaactcag ctcatgctct ggacatgacc tttgaggtgg atcccatgga tgagcccacc  21000 

ctgctttatc ttctcttcga agttttcgac gtggtcagag tgcatcagcc acaccgcggc  21060 

atcatcgagg cagtctacct gcgtacaccg ttctcggccg gtaacgctac cacgtaagaa  21120 

gcttcttgct tcttgcaaat agcagctgca accatggcct gcggatccca aaacggctcc  21180 

agcgagcaag agctcagagc cattgtccaa gacctgggtt gcggacccta ttttttggga  21240 

acctacgata agcgcttccc ggggttcatg gcccccgata agctcgcctg tgccattgta  21300 

aatacggccg gacgtgagac ggggggagag cactggttgg ctttcggttg gaacccacgt  21360 

tctaacacct gctacctttt tgatcctttt ggattctcgg atgatcgtct caaacagatt  21420 

taccagtttg aatatgaggg tctcctgcgc cgcagcgctc ttgctaccaa ggaccgctgt  21480 

attacgctgg aaaaatctac ccagaccgtg cagggccccc gttctgccgc ctgcggactt  21540 

ttctgctgca tgttccttca cgcctttgtg cactggcctg accgtcccat ggacggaaac  21600 

cccaccatga aattgctaac tggagtgcca aacaacatgc ttcattctcc taaagtccag  21660 

cccaccctgt gtgacaatca aaaagcactc taccattttc ttaataccca ttcgccttat  21720 

tttcgctctc atcgtacaca catcgaaagg gccactgcgt tcgaccgtat ggatgttcaa  21780 

taatgactca tgtaaacaac gtgttcaata aacatcactt tattttttta catgtatcaa  21840 

ggctctggat tacttattta tttacaagtc gaatgggttc tgacgagaat cagaatgacc  21900 

cgcaggcagt gatacgttgc ggaactgata cttgggttgc cacttgaatt cgggaatcac  21960 

caacttggga accggtatat cgggcaggat gtcactccac agctttctgg tcagctgcaa  22020 

agctccaagc aggtcaggag ccgaaatctt gaaatcacaa ttaggaccag tgctctgagc  22080 

gcgagagttg cggtacaccg gattgcagca ctgaaacacc atcagcgacg gatgtctcac  22140 

gcttgccagc acggtgggat ctgcaatcat gcccacatcc agatcttcag cattggcaat  22200 

gctgaacggg gtcatcttgc aggtctgcct acccatggcg ggcacccaat taggcttgtg  22260 

gttgcaatcg cagtgcaggg ggatcagtat catcttggcc tgatcctgtc tgattcctgg  22320 

atacacggct ctcatgaaag catcatattg cttgaaagcc tgctgggctt tactaccctc  22380 

ggtataaaac atcccgcagg acctgctcga aaactggtta gctgcacagc cggcatcatt  22440 

cacacagcag cgggcgtcat tgttggctat ttgcaccaca cttctgcccc agcggttttg  22500 

ggtgattttg gttcgctcgg gattctcctt taaggctcgt tgtccgttct cgctggccac  22560 

atccatctcg ataatctgct ccttctgaat cataatattg ccatgcaggc acttcagctt  22620 

gccctcataa tcattgcagc catgaggcca caacgcacag cctgtacatt cccaattatg  22680 

gtgggcgatc tgagaaaaag aatgtatcat tccctgcaga aatcttccca tcatcgtgct  22740 

cagtgtcttg tgactagtga aagttaactg gatgcctcgg tgctcttcgt ttacgtactg  22800 

gtgacagatg cgcttgtatt gttcgtgttg ctcaggcatt agtttaaaac aggttctaag  22860 

ttcgttatcc agcctgtact tctccatcag cagacacatc acttccatgc ctttctccca  22920 

agcagacacc aggggcaagc taatcggatt cttaacagtg caggcagcag ctcctttagc  22980 

cagagggtca tctttagcga tcttctcaat gcttcttttg ccatccttct caacgatgcg  23040 

cacgggcggg tagctgaaac ccactgctac aagttgcgcc tcttctcttt cttcttcgct  23100 

gtcttgactg atgtcttgca tggggatatg tttggtcttc cttggcttct ttttgggggg  23160 

tatcggagga ggaggactgt cgctccgttc cggagacagg gaggattgtg acgtttcgct  23220 

caccattacc aactgactgt cggtagaaga acctgacccc acacggcgac aggtgttttt  23280 

cttcgggggc agaggtggag gcgattgcga agggctgcgg tccgacctgg aaggcggatg  23340 

actggcagaa ccccttccgc gttcgggggt gtgctccctg tggcggtcgc ttaactgatt  23400 

tccttcgcgg ctggccattg tgttctccta ggcagagaaa caacagacat ggaaactcag  23460 

ccattgctgt caacatcgcc acgagtgcca tcacatctcg tcctcagcga cgaggaaaag  23520 

gagcagagct taagcattcc accgcccagt cctgccacca cctctaccct agaagataag  23580 

gaggtcgacg catctcatga catgcagaat aaaaaagcga aagagtctga gacagacatc  23640 

gagcaagacc cgggctatgt gacaccggtg gaacacgagg aagagttgaa acgctttcta  23700 

gagagagagg atgaaaactg cccaaaacag cgagcagata actatcacca agatgctgga  23760 

aatagggatc agaacaccga ctacctcata gggcttgacg gggaagacgc gctccttaaa  23820 

catctagcaa gacagtcgct catagtcaag gatgcattat tggacagaac tgaagtgccc  23880 

atcagtgtgg aagagctcag ctgcgcctac gagcttaacc ttttttcacc tcgtactccc  23940 

cccaaacgtc agccaaacgg cacctgcgag ccaaatcctc gcttaaactt ttatccagct  24000 

tttgctgtgc cagaagtact ggctacctat cacatctttt ttaaaaatca aaaaattcca  24060 

gtctcctgcc gcgctaatcg cacccgcgcc gatgccctac tcaatctggg acctggttca  24120 

cgcttacctg atatagcttc cttggaagag gttccaaaga tcttcgaggg tctgggcaat  24180 

aatgagactc gggccgcaaa tgctctgcaa aagggagaaa atggcatgga tgagcatcac  24240 

agcgttctgg tggaattgga aggcgataat gccagactcg cagtactcaa gcgaagcgtc  24300 

gaggtcacac acttcgcata tcccgctgtc aacctgcccc ctaaagtcat gacggcggtc  24360 

atggaccagt tactcattaa gcgcgcaagt cccctttcag aagacatgca tgacccagat  24420 

gcctgtgatg agggtaaacc agtggtcagt gatgagcagc taacccgatg gctgggcacc  24480 

gactctcccc gggatttgga agagcgtcgc aagcttatga tggccgtggt gctggttacc  24540 

gtagaactag agtgtctccg acgtttcttt accgattcag aaaccttgcg caaactcgaa  24600 

gagaatctgc actacacttt tagacacggc tttgtgcggc aggcatgcaa gatatctaac  24660 

gtggaactca ccaacctggt ttcctacatg ggtattctgc atgagaatcg cctaggacaa  24720 

agcgtgctgc acagcaccct taagggggaa gcccgccgtg attacatccg cgattgtgtc  24780 

tatctctacc tgtgccacac gtggcaaacc ggcatgggtg tatggcagca atgtttagaa  24840 

gaacagaact tgaaagagct tgacaagctc ttacagaaat ctcttaaggt tctgtggaca  24900 

gggttcgacg agcgcaccgt cgcttccgac ctggcagacc tcatcttccc agagcgtctc  24960 

agggttactt tgcgaaacgg attgcctgac tttatgagcc agagcatgct taacaatttt  25020 

cgctctttca tcctggaacg ctccggtatc ctgcccgcca cctgctgcgc actgccctcc  25080 

gactttgtgc ctctcaccta ccgcgagtgc cccccgccgc tatggagtca ctgctacctg  25140 

ttccgtctgg ccaactatct ctcctaccac tcggatgtga tcgaggatgt gagcggagac  25200 

ggcttgctgg agtgccactg ccgctgcaat ctgtgcacgc cccaccggtc cctagcttgc  25260 

aacccccagt tgatgagcga aacccagata ataggcacct ttgaattgca aggccccagc  25320 

agccaaggcg atgggtcttc tcctgggcaa agtttaaaac tgaccccggg actgtggacc  25380 

tccgcctact tgcgcaagtt tgctccggaa gattaccacc cctatgaaat caagttctat  25440 

gaggaccaat cacagcctcc aaaggccgaa ctttcggctt gcgtcatcac ccagggggca  25500 

attctggccc aattgcaagc catccaaaaa tcccgccaag aatttctact gaaaaagggt  25560 

aagggggtct accttgaccc ccagaccggc gaggaactca acacaaggtt ccctcaggat  25620 

gtcccaacga cgagaaaaca agaagttgaa ggtgcagccg ccgcccccag aagatatgga  25680 

ggaagattgg gacagtcagg cagaggaggc ggaggaggac agtctggagg acagtctgga  25740 

ggaagacagt ttggaggagg aaaacgagga ggcagaggag gtggaagaag taaccgccga  25800 

caaacagtta tcctcggctg cggagacaag caacagcgct accatctccg ctccgagtcg  25860 

aggaacccgg cggcgtccca gcagtagatg ggacgagacc ggacgcttcc cgaacccaac  25920 

cagcgcttcc aagaccggta agaaggatcg gcagggatac aagtcctggc gggggcataa  25980 

gaatgccatc atctcctgct tgcatgagtg cgggggcaac atatccttca cgcggcgcta  26040 

cttgctattc caccatgggg tgaactttcc gcgcaatgtt ttgcattact accgtcacct  26100 

ccacagcccc tactatagcc agcaaatccc gacagtctcg acagataaag acagcggcgg  26160 

cgacctccaa cagaaaacca gcagcggcag ttagaaaata cacaacaagt gcagcaacag  26220 

gaggattaaa gattacagcc aacgagccag cgcaaacccg agagttaaga aatcggatct  26280 

ttccaaccct gtatgccatc ttccagcaga gtcggggtca agagcaggaa ctgaaaataa  26340 

aaaaccgatc tctgcgttcg ctcaccagaa gttgtttgta tcacaagagc gaagatcaac  26400 

ttcagcgcac tctcgaggac gccgaggctc tcttcaacaa gtactgcgcg ctgactctta  26460 

aagagtaggc agcgaccgcg cttattcaaa aaaggcggga attacatcat cctcgacatg  26520 

agtaaagaaa ttcccacgcc ttacatgtgg agttatcaac cccaaatggg attggcagca  26580 

ggcgcctccc aggactactc cacccgcatg aattggctca gcgccgggcc ttctatgatt  26640 

tctcgagtta atgatatacg cgcctaccga aaccaaatac ttttggaaca gtcagctctt  26700 

accaccacgc cccgccaaca ccttaatccc agaaattggc ccgccgccct agtgtaccag  26760 

gaaagtcccg ctcccaccac tgtattactt cctcgagacg cccaggccga agtccaaatg  26820 

actaatgcag gtgcgcagtt agctggcggc tccaccctat gtcgtcacag gcctcggcat  26880 

aatataaaac gcctgatgat cagaggccga ggtatccagc tcaacgacga gtcggtgagc  26940 

tctccgcttg gtctacgacc agacggaatc tttcagattg ccggctgcgg gagatcttcc  27000 

ttcacccctc gtcaggctgt tctgactttg gaaagttcgt cttcgcaacc ccgctcgggc  27060 

ggaatcggga ccgttcaatt tgtagaggag tttactccct ctgtctactt caaccccttc  27120 

tccggatctc ctgggcacta cccggacgag ttcataccga acttcgacgc gattagcgag  27180 

tcagtggacg gctacgattg atgtctggtg acgcggctga gctatctcgg ctgcgacatc  27240 

tagaccactg ccgccgcttt cgctgctttg cccgggaact tattgagttc atctacttcg  27300 

aactccccaa ggatcaccct caaggtccgg cccacggagt gcggattact atcgaaggca  27360 

aaatagactc tcgcctgcaa cgaattttct cccagcggcc cgtgctgatc gagcgagacc  27420 

agggaaacac cacggtttcc atctactgca tttgtaatca ccccggattg catgaaagcc  27480 

tttgctgtct tatgtgtact gagtttaata aaaactgaat taagactctc ctacggactg  27540 

ccgcttcttc aacccggatt ttacaaccag aagaacaaaa cttttcctgt cgtccaggac  27600 

tctgttaact tcacctttcc tactcacaaa ctagaagctc aacgactaca ccgcttttcc  27660 

agaagcattt tccctactaa tactactttc aaaaccggag gtgagctcca cggtctccct  27720 

acagaaaacc cttgggtgga agcgggcctt gtagtactag gaattcttgc gggtgggctt  27780 

gtgattattc tttgctacct atacacacct tgcttcactt tcctagtggt gttgtggtat  27840 

tggtttaaaa aatggggccc atactagtct tgcttgtttt actttcgctt ttggaaccgg  27900 

gttctgccaa ttacgatcca tgtctagact ttgacccaga aaactgcaca cttacttttg  27960 

cacccgacac aagccgcatc tgtggagttc ttattaagtg cggatgggaa tgcaggtccg  28020 

ttgaaattac acacaataac aaaacctgga acaatacctt atccaccaca tgggagccag  28080 

gagttcccga gtggtacact gtctctgtcc gaggtcctga cggttccatc cgcattagta  28140 

acaacacttt cattttttct gaaatgtgcg atctggccat gttcatgagc aaacagtatt  28200 

ctctatggcc tcctagcaag gacaacatcg taacgttctc cattgcttat tgcttgtgcg  28260 

cttgccttct tactgcttta ctgtgcgtat gcatacacct gcttgtaacc actcgcatca  28320 

aaaacgccaa taacaaagaa aaaatgcctt aacctctttc tgtttacaga catggcttct  28380 

cttacatctc tcatatttgt cagcattgtc actgccgctc acggacaaac agtcgtctct  28440 

atcccactag gacataatta cactctcata ggacccccaa tcacttcaga ggtcatctgg  28500 

accaaactgg gaagcgttga ttactttgat ataatctgta acaaaacaaa accaataata  28560 

gtaacttgca acatacaaaa tcttacattg attaatgtta gcaaagttta cagcggttac  28620 

tattatggtt atgacagata cagtagtcaa tatagaaatt acttggttcg tgttacccag  28680 

ttgaaaacca cgaaaatgcc aaatatggca aagattcgat ccgatgacaa ttctctagaa  28740 

acttttacat ctcccaccac acccgacgaa aaaaacatcc cagattcaat gattgcaatt  28800 

gttgcagcgg tggcagtggt gatggcacta ataataatat gcatgctttt atatgcttgt  28860 

cgctacaaaa agtttcatcc taaaaaacaa gatctcctac taaggcttaa catttaattt  28920 

ctttttatac agccatggtt tccactacca cattccttat gcttactagt ctcgcaactc  28980 

tgacttctgc tcgctcacac ctcactgtaa ctataggctc aaactgcaca ctaaaaggac  29040 

ctcaaggtgg tcatgtcttt tggtggagaa tatatgacaa tggatggttt acaaaaccat  29100 

gtgaccaacc tggtagattt ttctgcaacg gcagagacct aaccattatc aacgtgacag  29160 

caaatgacaa aggcttctat tatggaaccg actataaaag tagtttagat tataacatta  29220 

ttgtactgcc atctaccact ccagcacccc gcacaactac tttctctagc agcagtgtcg  29280 

ctaacaatac aatttccaat ccaacctttg ccgcgctttt aaaacgcact gtgaataatt  29340 

ctacaacttc acatacaaca atttccactt caacaatcag catcatcgct gcagtgacaa  29400 

ttggaatatc tattcttgtt tttaccataa cctactacgc ctgctgctat agaaaagaca  29460 

aacataaagg tgatccatta cttagatttg atatttaatt tgttcttttt ttttatttac  29520 

agtatggtga acaccaatca tggtacctag aaatttcttc ttcaccatac tcatctgtgc  29580 

ttttaatgtt tgcgctactt tcacagcagt agccacagca accccagact gtataggagc  29640 

atttgcttcc tatgcacttt ttgcttttgt tacttgcatc tgcgtatgta gcatagtctg  29700 

cctggttatt aattttttcc aacttctaga ctggatcctt gtgcgaattg cctacctgcg  29760 

ccaccatccc gaataccgca accaaaatat cgcggcactt cttagactca tctaaaacca  29820 

tgcaggctat actaccaata tttttgcttc tattgcttcc ctacgctgtc tcaaccccag  29880 

ctgcctatag tactccacca gaacacctta gaaaatgcaa attccaacaa ccgtggtcat  29940 

ttcttgcttg ctatcgagaa aaatcagaaa tccccccaaa tttaataatg attgctggaa  30000 

taattaatat aatctgttgc accataattt catttttgat atacccccta tttgattttg  30060 

gctggaatgc tcccaatgca catgatcatc cacaagaccc agaggaacac attcccccac  30120 

aaaacatgca acatccaata gcgctaatag attacgaaag tgaaccacaa cccccactac  30180 

tccctgctat tagttacttc aacctaaccg gcggagatga ctgaaacact caccacctcc  30240 

aattccgccg aggatctgct cgatatggac ggccgcgtct cagaacaacg acttgcccaa  30300 

ctacgcatcc gccagcagca ggaacgcgtg gccaaagagc tcagagatgt catccaaatt  30360 

caccaatgca aaaaaggcat attctgtttg gtaaaacaag ccaagatatc ctacgagatc  30420 

accgctactg accatcgcct ctcttacgaa cttggccccc aacgacaaaa atttacctgc  30480 

atggtgggaa tcaaccccat agttatcacc caacaaagtg gagatactaa gggttgcatt  30540 

cactgctcct gcgattccat cgagtgcacc tacaccctgc tgaagaccct atgcggccta  30600 

agagacctgc taccaatgaa ttaaaaaaaa atgattaata aaaaatcact tacttgaaat  30660 

cagcaataag gtctctgttg aaattttctc ccagcagcac ctcacttccc tcttcccaac  30720 

tctggtattc taaaccccgt tcagcggcat actttctcca tactttaaag gggatgtcaa  30780 

attttagctc ctctcctgta cccacaatct tcatgtcttt cttcccagat gaccaagaga  30840 

gtccggctca gtgactcctt caaccctgtc tacccctatg aagatgaaag cacctcccaa  30900 

caccccttta taaacccagg gtttatttcc ccaaatggct tcacacaaag cccagacgga  30960 

gttcttactt taaaatgttt aaccccacta acaaccacag gcggatctct acagctaaaa  31020 

gtgggagggg gacttacagt ggatgacact gatggtacct tacaagaaaa catacgtgct  31080 

acagcaccca ttactaaaaa taatcactct gtagaactat ccattggaaa tggattagaa  31140 

actcaaaaca ataaactatg tgccaaattg ggaaatgggt taaaatttaa caacggtgac  31200 

atttgtataa aggatagtat taacacctta tggactggaa taaaccctcc acctaactgt  31260 

caaattgtgg aaaacactaa tacaaatgat ggcaaactta ctttagtatt agtaaaaaat  31320 

ggagggcttg ttaatggcta cgtgtctcta gttggtgtat cagacactgt gaaccaaatg  31380 

ttcacacaaa agacagcaaa catccaatta agattatatt ttgactcttc tggaaatcta  31440 

ttaactgagg aatcagactt aaaaattcca cttaaaaata aatcttctac agcgaccagt  31500 

gaaactgtag ccagcagcaa agcctttatg ccaagtacta cagcttatcc cttcaacacc  31560 

actactaggg atagtgaaaa ctacattcat ggaatatgtt actacatgac tagttatgat  31620 

agaagtctat ttcccttgaa catttctata atgctaaaca gccgtatgat ttcttccaat  31680 

gttgcctatg ccatacaatt tgaatggaat ctaaatgcaa gtgaatctcc agaaagcaac  31740 

atagctacgc tgaccacatc cccctttttc ttttcttaca ttacagaaga cgacaactaa  31800 

aataaagttt aagtgttttt atttaaaatc acaaaattcg agtagttatt ttgcctccac  31860 

cttcccattt gacagaatac accaatctct ccccacgcac agctttaaac atttggatac  31920 

cattagagat agacattgtt ttagattcca cattccaaac agtttcagag cgagccaatc  31980 

tggggtcagt gatagataaa aatccatcgc gatagtcttt taaagcgctt tcacagtcca  32040 

actgctgcgg atgcgactcc ggagtttgga tcacggtcat ctggaagaag aacgatggga  32100 

atcataatcc gaaaacggta tcggacgatt gtgtctcatc aaacccacaa gcagccgctg  32160 

tctgcgtcgc tccgtgcgac tgctgtttat gggatcaggg tccacagttt cctgaagcat  32220 

gattttaata gcccttaaca tcaactttct ggtgcgatgc gcgcagcaac gcattctgat  32280 

ttcactcaaa tctttgcagt aggtacaaca cattattaca atattgttta ataaaccata  32340 

attaaaagcg ctccagccaa aactcatatc tgatataatc gcccctgcat gaccatcata  32400 

ccaaagttta atataaatta aatgacgttc cctcaaaaac acactaccca catacatgat  32460 

ctcttttggc atgtgcatat taacaatctg tctgtaccat ggacaacgtt ggttaatcat  32520 

gcaacccaat ataaccttcc ggaaccacac tgccaacacc gctcccccag ccatgcattg  32580 

aagtgaaccc tgctgattac aatgacaatg aagaacccaa ttctctcgac cgtgaatcac  32640 

ttgagaatga aaaatatcta tagtggcaca acatagacat aaatgcatgc atcttctcat  32700 

aatttttaac tcctcaggat ttagaaacat atcccaggga ataggaagct cttgcagaac  32760 

agtaaagctg gcagaacaag gaagaccacg aacacaactt acactatgca tagtcatagt  32820 

atcacaatct ggcaacagcg ggtggtcttc agtcatagaa gctcgggttt cattttcctc  32880 

acaacgtggt aactgggctc tggtgtaagg gtgatgtctg gcgcatgatg tcgagcgtgc  32940 

gcgcaacctt gtcataatgg agttgcttcc tgacattctc gtattttgta tagcaaaacg  33000 

cggccctggc agaacacact cttcttcgcc ttctatcctg ccgcttagcg tgttccgtgt  33060 

gatagttcaa gtacagccac actcttaagt tggtcaaaag aatgctggct tcagttgtaa  33120 

tcaaaactcc atcgcatcta attgttctga ggaaatcatc cacggtagca tatgcaaatc  33180 

ccaaccaagc aatgcaactg gattgcgttt caagcaggag aggagaggga agagacggaa  33240 

gaaccatgtt aatttttatt ccaaacgatc tcgcagtact tcaaattgta gatcgcgcag  33300 

atggcatctc tcgcccccac tgtgttggtg aaaaagcaca gctaaatcaa aagaaatgcg  33360 

attttcaagg tgctcaacgg tggcttccaa caaagcctcc acgcgcacat ccaagaacaa  33420 

aagaatacca aaagaaggag cattttctaa ctcctcaatc atcatattac attcctgcac  33480 

cattcccaga taattttcag ctttccagcc ttgaattatt cgtgtcagtt cttgtggtaa  33540 

atccaatcca cacattacaa acaggtcccg gagggcgccc tccaccacca ttcttaaaca  33600 

caccctcata atgacaaaat atcttgctcc tgtgtcacct gtagcgaatt gagaatggca  33660 

acatcaattg acatgccctt ggctctaagt tcttctttaa gttctagttg taaaaactct  33720 

ctcatattat caccaaactg cttagccaga agccccccgg gaacaagagc aggggacgct  33780 

acagtgcagt acaagcgcag acctccccaa ttggctccag caaaaacaag attggaataa  33840 

gcatattggg aaccaccagt aatatcatcg aagttgctgg aaatataatc aggcagagtt  33900 

tcttgtagaa attgaataaa agaaaaattt gccaaaaaaa cattcaaaac ctctgggatg  33960 

caaatgcaat aggttaccgc gctgcgctcc aacattgtta gttttgaatt agtctgcaaa  34020 

aataaaaaaa aaacaagcgt catatcatag tagcctgacg aacaggtgga taaatcagtc  34080 

tttccatcac aagacaagcc acagggtctc cagctcgacc ctcgtaaaac ctgtcatcgt  34140 

gattaaacaa cagcaccgaa agttcctcgc ggtgaccagc atgaataagt cttgatgaag  34200 

catacaatcc agacatgtta gcatcagtta aggagaaaaa acagccaaca tagcctttgg  34260 

gtataattat gcttaatcgt aagtatagca aagccacccc tcgcggatac aaagtaaaag  34320 

gcacaggaga ataaaaaata taattatttc tctgctgctg tttaggcaac gtcgcccccg  34380 

gtccctctaa atacacatac aaagcctcat cagccatggc ttaccagaga aagtacagcg  34440 

ggcacacaaa ccacaagctc taaagtcact ctccaacctc tccacaatat atatacacaa  34500 

gccctaaact gacgtaatgg gactaaagtg taaaaaatcc cgccaaaccc aacacacacc  34560 

ccgaaactgc gtcaccaggg aaaagtacag tttcacttcc gcaatcccaa caagcgtcac  34620 

ttcctctttc tcacggtacg tcacatccca ttaacttaca acgtcatttt cccacggccg  34680 

cgccgcccct tttaaccgtt aaccccacag ccaatcacca cacggcccac actttttaaa  34740 

atcacctcat ttacatattg gcaccattcc atctataagg tatattattg atgatg      34796