PATENT DOCUMENT

Abstract:
Secretome profiling improves the pregnancy success rate of in vitro fertilization processes, while reducing the risk of multiple births.

Full Description:
RELATED APPLICATIONS 
     This application is a divisional of U.S. application Ser. No. 15/052,475, filed Feb. 24, 2016, issued on Jul. 25, 2017 as U.S. Pat. No. 9,714,950, which is a continuation of U.S. application Ser. No. 13/081,463, filed Apr. 6, 2011, which claims benefit of priority to U.S. Provisional Patent Application No. 61/321,448 filed Apr. 6, 2010, each of which is incorporated by reference to the same extent as though fully replicated herein. 
    
    
     SEQUENCE LISTING 
     This application includes a Sequence Listing, as set forth in an ASCII-compliant text file named “CCRMProtein_ST25.txt”, created on Apr. 6, 2011, and containing 2263 kilobytes, which is incorporated by reference to the same extent as though fully replicated herein. 
     BACKGROUND 
     Field 
     The invention relates to the field of in vitro fertilization (IVF), which is a process by which mammalian egg cells are fertilized by sperm outside the womb. More particularly, evaluation of a secretome profile is used to enhance the pregnancy success rate when the fertilized egg is implanted into a patient&#39;s uterus. 
     Description of the Related Art 
     IVF infertility treatment offers infertile couples a chance to have a biologically related child. IVF may overcome female infertility due to problems of the fallopian tube or endometriosis. IVF also overcomes male infertility due to problems with sperm quality or quantity. The IVF process involves hormonally controlling the ovulatory process, removing eggs (termed ova) from the woman&#39;s ovaries and permitting the sperm to fertilize the eggs in a fluid medium. The fertilized egg, termed an embryo, is subsequently transferred to the patient&#39;s uterus with the intent of establishing a successful pregnancy. Due to expensive procedural costs, IVF is only attempted after the failure of less expensive fertility treatments. 
       FIG. 1  displays a schematic for mammalian blastocyst implantation. Mammalian embryo implantation is a complex and intricate process involving numerous biological changes at both the embryo and endometrial level. The interaction between the blastocyst and the endometrium is a function of both a receptive endometrial environment and a healthy blastocyst. A blastocyst failing to implant or an endometrium failing to sustain growth and differentiation will result in spontaneous abortion. The prior art teaches very little about the embryo&#39;s role in the events leading to the attachment of a viable blastocyst to a receptive uterine luminal epithelium. 
     IVF treatment begins with administration of hormonal medications to stimulate ovarian follicle production, such as gonadotropins hormones. The prevention of spontaneous ovulation involves using other hormones, such as GnRH antagonists or GnRH agonists that block the natural surge of luteinizing hormone. With adequate follicular maturation, administration of human chorionic gonadotropin hormone causes ovulation approximately 42 hours after the administration. However, the egg retrieval procedure takes place just prior to ovulation, in order to recover the eggs from the ovary. The egg retrieval proceeds using a transvaginal technique involving an ultrasound-guided needle that pierces the vaginal wall to reach the ovaries. After recovery of the follicles through the needle, the follicular fluid is provided to the IVF laboratory to identify eggs. Typically, the procedure retrieves between 10 and 30 eggs. The retrieval procedure takes approximately 20 minutes and is usually done under conscious sedation or general anesthesia. 
     For IVF, the fertilization of the egg (termed insemination) proceeds in the laboratory where the identified eggs and semen are usually incubated together in a culture media. The confirmation of fertilization proceeds by monitoring the eggs for cell division. For instance, a fertilized egg may show two pronuclei. In certain situations, such as low sperm count or motility, a single sperm may be injected directly into the egg using a method called intracytoplasmic sperm injection (ICSI). In another option known as gamete intrafallopian transfer, eggs are removed from the woman and placed in one of the fallopian tubes, along with the man&#39;s sperm. In this example, fertilization occurs within the women&#39;s body, a process termed in vivo fertilization. 
     Selected embryos are transferred to the patient&#39;s uterus through a thin, plastic catheter, which goes through the vagina and cervix. Typically, transfer of 6-8 cell stage embryos to the uterus occurs three days after embryo retrieval. Alternatively, embryos can be placed into an extended culture system with a transfer done at the blastocyst stage at approximately five days post-retrieval. Blastocyst stage transfers often result in higher pregnancy rates. Additionally, embryonic cryopreservation, or the storage of embryos in a frozen state, is feasible until uterine transfer. For example, the first term pregnancy derived from a frozen human embryo was reported in 1984. 
     Despite progressively improving IVF pregnancy rates, the majority of transferred human embryos result in implantation failure. For example, Canadian clinics reported an average pregnancy rate of 35% for one cycle, but a live birth rate of only 27% in 2006. Moreover, implantation success rates may decrease with the increasing maternal age, if donor eggs are not used. Various factors are associated with implantation failure, including embryo chromosome aneuploidies related to advanced maternal age and maternal factors such as endometrium response failure to hormone regulation. 
     To overcome low implantation success rate, multiple embryos are commonly transferred during a single IVF procedure. The process for selecting embryos for transfer often involves grading methods developed in individual laboratories to judge oocyte and embryo quality. An arbitrary embryo score, involving the number and quality of embryos, may reveal the probability of pregnancy success post-transfer. For example, an embryologist may grade embryos using morphological qualities including the number of cells, clearness of cytoplasm, evenness of growth and degree of fragmentation. However, embryo selection based on morphological qualities is not precise. Often, several embryos selected for these general qualities are implanted to improve the chance of pregnancy. The number of embryos transferred depends upon the number available, the age of the woman and other health and diagnostic factors. 
     The transfer of multiple embryos, however, often results in multiple pregnancies, a major complication of IVF. In general, multiple pregnancies, specifically, more than twins, hold maternal and fetal risks. For example, multiple births are associated with increased risk of pregnancy loss, neonatal morbidity, obstetrical complications, and prematurity with potential for long term damage. Some countries implemented strict limits on the number of transferred embryos to reduce the risk of high-order multiples (e.g., triplets or more). However, these limitations are not universally followed or accepted. 
     SUMMARY 
     In one embodiment, a system for enhancing the pregnancy success rate of in vitro fertilization includes a means for determining the secretome profile of an embryo to identify proteins, polypeptides, oligopeptides or protein fragments implicated in implantation success and a means for recommending whether to implant the embryo, such as a blastocyst, on the basis of the secretome profile. 
     The CDC data from 2006 shows that use of donor eggs in a 40 year old woman will result in a live birth 54% of the time. This compares to 20.6% using the woman&#39;s own eggs. Thus, an opportunity exists for the instrumentalities of this disclosure to facilitate success rates approximating the use of donor eggs when using one&#39;s own eggs, and may even permit higher success rates due to better selection of viable embryos. 
     In an embodiment, a system is provided for enhancing the pregnancy success rate of in vitro fertilization. The system includes an electronic system to configured to gather secretome data as a secretome profile of an embryo by quantitating proteins implicated in implantation success. A model is provided for use in recommending whether to implant the embryo on the basis of this secretome profile. The secretome date may be, for example, provided by use of mass spectroscopy or ELISA measurements. 
     According to one aspect of the system, the secretome profile may be provided by using proteins as embryo secretions in culture media that may be linked to changed odds of implantation success, for example, as may be found in one or more sequences found in SEQID Nos. 1-404. The sequences of SEQID Nos. 261-404 are preferred for this use. Particularly preferred are the sequences of SEQID Nos. 310, 311, 313, 317, 318, 319, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 345, 346, 347, 348, 349, 350, 368, 371, 374, 383, 391, 397, 398, 399, 402, 403, and 404. The sequences of SEQID Nos. 310, 311, 313, 318, 319, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 345, 346, 347, 348, 349, 350, 368, 371, 374, 391, 397, 398, 399, 402, 403, and 404 are associated with increased odds of implantation success, whereas those of SEQID Nos. 317 and 383 are associated with failure or aneuploidy. 
     In an embodiment, a method of in vitro fertilization entails determining the secretome profile of a candidate embryo by use of these sequences to ascertain proteins implicated in implantation success. This provides data that may be submitted to a model that associates one or more of these proteins with changes odds of implantation success or failure. A recommendation for implantation of the embryo may then be provided based upon the modeling outcome. The embryo may be conditionally implanted on the basis of the recommendation. 
     In an embodiment, there is an improved ELISA assay kit with a plurality of microwells for the quantitation of protein content in a sample. The microwells are constructed and arranged to quantitate for a plurality of proteins selected from these sequences. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1  is a diagram illustrating the process blastocyst implantation. 
         FIG. 2  is a table that illustrates the success rates from donor oocyte IVF cycles. 
         FIG. 3  is a flow chart for a method of developing and using a predictive model that may be used to assess embryo viability as a candidate for use in an IVF implantation procedure. 
         FIG. 4  shows a system that analyzes the secretome of an embryo to assess viability. 
     
    
    
     DETAILED DESCRIPTION 
     The following definitions are provided to facilitate understanding of certain terms used herein and are not meant to limit the scope of the present disclosure. 
     The term “secretome panel” refers to a collection of individual proteins, polypeptides, oligopeptides or protein fragments that are differentially expressed and secreted by an embryo. The proteins, polypeptides, oligopeptides or protein fragments of the secretome panel are selected based on predictions of developmental competence and implantation potential of an embryo. 
     The term “spent media” refers to media surrounding an embryo that accumulates proteins secreted from the embryo. 
     The term “secretome profiling” refers to qualitative and quantitative analysis of a secretome panel collected from spent media. 
     There will now be shown and described a system for enhancing the pregnancy success rate of in vitro fertilization involving non-invasive secretome profiling of an embryo. Secretome profiling may be performed on embryos at differing embryonic developmental stages, such as day one through day six embryos. 
     Non-limiting examples of proteins comprising the secretome panel include proteins involved in amino acid metabolism, lipid metabolism, carbohydrate metabolism, signal transduction, apoptosis, transcription and combinations thereof. In one example, an embryo secretes a protein that is involved in amino acid metabolism, thereby indicating potential for successful embryo implantation. In another example, an embryo secretes a protein that is involved in apoptosis, thereby indicating a reduced potential for successful blastocyst implantation. Nonlimiting examples of the number of proteins comprising the secretome panel includes ≧250 proteins, ≧100 proteins, ≧50 proteins, ≧20 proteins or ≧10 proteins. 
     Secretome profiling involves assessment of a secretome panel. Secretome profiling is a noninvasive method for predicting developmental competence and viability of an embryo. Comparison of secretome profiles between developing embryos and degenerating embryos, both at the same developmental stage, reveals significant differences in protein expression. Moreover, secretome profiling provides a molecular perspective of the functioning biochemical pathways present during embryo development. A noninvasive secretome profiling assay correlates embryonic secretome to embryonic viability, thereby facilitating single embryo transfer during in vitro fertilization. 
       FIG. 2  highlights the importance of embryo developmental competence by showing high success rates from donor oocyte IVF cycles using young reproductive age oocytes transferred to an advanced maternal age endometrium. The data is provided for a Colorado clinic from 2004-2009 including outcomes for over 1000 donor oocyte cycles. These results demonstrate a 66.6% implantation rate for IVF recipients with an average endometrial age of 40.6 years and donor oocytes with an average age of 26.6 years. 
     In one embodiment, secretome profiling occurs by assessing a secretome panel via Enzyme-Linked Immunosorbent Assay (ELISA). In another embodiment, secretome profiling proceeds by assessing a secretome panel via mass spectrometry. 
     Non-limiting examples of solid-state substrates used in ELISA include microwell plates, such as 96-well plates, 384-well plates and 8-well strips, microarray slides and nitrocellulose membranes. In one embodiment, glass microarray surfaces contain chemical functional groups such as epoxy, amine or aldehyde. In one embodiment, microwell plate material comprises polystyrene. 
     The term “capture antibody” refers to an antibody secured, either covalently or non-covalently, to a solid-state substrate. The capture antibody recognizes and binds to a specific antigen such as a protein, a polypeptide, an oligopeptide, a protein fragment, a carbohydrate or a small molecule. In one example, a solid-state substrate is functionalized with capture antibodies by passive adsorption or by specific binding. For example, specific binding of capture antibodies may occur using biotinylated capture antibodies and streptavidin-coated solid-state substrates. In another example, solid-state substrates are functionalized with antigens via passive adsorption. 
     Non-limiting examples of ELISA types include direct, indirect, competitive, and sandwich. In one example, proteins from the secretome panel are detected on a solid-state substrate using either a primary labeled antibody or a secondary labeled antibody. In another example, proteins from the secretome panels are detecting using a sandwich ELISA wherein a protein from the secretome panel is bound between two primary antibodies, namely the capture antibody and the detection antibody. Non-limiting examples of antibody labels include enzymes and fluororphores. In one embodiment, an enzyme that is conjugated to a detection antibody binds to a substrate producing either a chromogenic, fluorescent or chemiluminescent signal that is proportional to the quantity of protein from the secretome panel. 
       FIG. 3  shows a method  300  implementing the concepts described above to identify those embryos having the greatest chanced for implantation success rates. Step  302  entails ascertaining the secretome of proteins secreted into culture medium by an egg, blastocyst or developing embryo (hereinafter “embryo”) that has been previously fertilized by methods known to the art. Step  302  may be performed at any time, including before an egg is fertilized or immediately thereafter. More typical times to sample the media include form one to five days after fertilization occurs. Step  302  is preferably conducted before step  304 , but samples of the culture medium may be frozen for later analysis. The embryo is then used  304  in an IVF procedure attempting to implant the embryo in a woman who desires to have a baby. The implantation successes and failures are monitored  306  to collect data relating the successes and failures to one or more proteins of the secretome. Once a sufficient number of instances have transpired  308  to provide for statistical significance, the proteins are mapped  310  to the instances of success and failure. The exact manner of mapping is not of particular importance, as there a number of biostatistical analysis software products are commercially available. For example, this may be done using the JMP® 9 packages from SAS of Cary, N.C. to implement methods as taught by Zar, Biostatistical Analysis, 5th Ed., Prentice Hall (2007) to map the secretome profiles using also time as a variable. The mapping may be multivariate, or by an artificially intelligent algorithm, such as a neural network. This model may be then used  312  as a predictive tool to select embryos for implantation based upon their secretome profile. This is done by analyzing  314  the secretome of candidate embryos for implantation with use of the model to predict implantation success or failure. One or more of the selected embryos are then implanted  316   
       FIG. 4  shows an analytical system  400  that may be used in step  314  according to one embodiment. A specimen  402  contains a sample of culture medium that is collected for secretome analysis as described above. By way of example, this specimen may contain culture medium in the form of a single or multi-well ELISA assay, or in a form that has been pre-processed for mass spectroscopy analysis. A corresponding measurement device  404  obtains primary measurements from the specimen  402 . The measurements are representative of one or more proteins in the secretome that have been mapped to incidences of implantation success of failure. The model for this mapping is provided in step  312  of method  300  and resides on analyzer  406 , which may be a computer operating on program instructions. The analyzer  406  receives signals from the measurement device  404  and interprets these by computational analysis according to the model then provides the results of computational analysis to an output device  408 , which may be printer or an optical display. The various components of system  400  may be partially integrated, such as by combining the measurement device  404 , analyzer  406 , and output device  408  into one integrated system. 
     It will be appreciated that perceptive use of the instrumentalities described herein may result in a better selection of healthy embryos, such as blastocysts, for implantation. Thus, fewer blastocysts need to be implemented, such that there is lower risk of multiple pregnancies while achieving a higher overall pregnancy success rate. 
     The following descriptions will show and describe, by way of non-limiting examples, a process for improving pregnancy success rates with lower incidence of multiple births. The following examples describe secretome profiling of spent media from human blastocysts to provide implantation recommendation. It is to be understood that these examples are provided by way of illustration and should not be unduly construed to limit the scope of what is disclosed herein. 
     Example 1 
     Secretome Profiling of Human Embryos Using Mass Spectrometry 
     The following nonlimiting example teaches by way of illustration, not by limitation, secretome profiling of a human embryo using mass spectrometry (MS). Human cleavage-stage embryos were cultured in 10 μL drops of G1 supplemented with 2.5 mg/mL recombinant albumin under oil at 37° C., 6% CO 2 , 5% O 2  for 24 hours. The embryos were washed twice in G2 culture media and further cultured in 10 μL drops of G2 supplemented with 2.5 mg/mL recombinant albumin under oil at 37° C., 6% CO 2 , 5% O 2  for 48 hours with a fresh drop of G2 media added after 24 hours. Spent media samples of blastocysts were transferred into 0.65 mL Eppendorf tubes. Control groups comprised media cultured and collected under the same conditions but without embryos. 
     Micro-drops of spent media were depleted of human serum albumin (HSA) using Cibracron Blue Activated SwellGel Discs (Themo Fisher Scientific, Rockford, Ill.). The proteins in the spent media were separated by  1 D gel electrophoresis (Invitrogen, Carlsbad, Calif.), followed by Coomassie staining. Twenty five individual bands were cut out from each sample lane and a standard in-gel digestion protocol was used based on previously used methods ((1.) Rosenfeld, J., Capdevielle, J., Guillemot, J. C. &amp; Ferrara, P. In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis. Anal. Biochem. 203, 173-179 (1992), (2.) Hellman, U., Wernstedt, C., Gonez, J. &amp; Heldin, C. H. Improvement of an “In-Gel” digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing. Anal. Biochem. 224, 451-455 (1995)). Iodoacteamide (IAM) was used for cysteine alkylation. The samples were analyzed on a LTQ-FT Ultra Hybrid Mass Spectrometer (Thermo/Finnigan; Waltham, Mass.) with a method based on a previously described protocol from Hansen et al., (Hansen KC, Kiemele L, Mailer O, O&#39;Brien J, Shankar A, Fornetti J, Schedin P. An in-solution ultrasonication-assisted digestion method for improved extracellular matrix proteome coverage. Mol Cell Proteomics. 8(7):1648-57 (2009)). 
     Spent media samples were analyzed on a LTQ-FT Ultra Hybrid Mass Spectrometer (Thermo/Finnigan; Waltham, Mass.). Peptide desalting and separation was achieved using a dual capillary/nano pump HPLC system (Agilent 1200, Palo Alto, Calif.). On this system 8 μL of spent media sample was loaded onto a trapping column (ZORBAX 300SB-C18, dimensions 5×0.3 mm 5 um) and washed with 5% acetonitrile (ACN), 0.1% formic acid (FA) at a flow rate of 15 μL/min for 5 minutes. At this time, the trapping column was put online with the nano-pump at a flow rate of 350 nL/min. An 85 minute gradient, from 8% ACN to 40% ACN, was used to separate the peptides. The column was made from an in-house pulled 360/100 nm (outer/inner diameter) fused silica capillary packed with Jupiter C18 resin (Penomenex; Torrance, Calif.). The column was kept at a constant 40° C. using an in-house built column heater. Data acquisition was performed using the instrument supplied Xcalibur (version 2.0.6) software. The LC runs were monitored in positive ion mode by sequentially recording survey MS scans (m/z 400-2000), in the ICR cell, while three MS 2  were obtained in the ion trap via CID for the most intense ions. After two acquisitions of a given ion within 45 seconds, the ion was excluded for 150 seconds. 
     For data analysis, The Raw Distiller program (UCSF) was used to create a de-isotoped centroided peak lists from the raw spectra into the mascot format using the default settings. The peak lists were searched against the SwissProt Human database (51.6,  Homo sapiens  15720 sequences) using Mascot™ server (Version 2.2, Matrix Science, Boston, Mass.). The search parameters are the same as those followed in Hansen et al., (Hansen K C, Kiemele L, Maller O, O&#39;Brien J, Shankar A, Fornetti J, Schedin P. An in-solution ultrasonication-assisted digestion method for improved extracellular matrix proteome coverage. Mol Cell Proteomic. 8(7): 1648-57 (2009)). The Mascot results were loaded into Scaffold (v 2.06) and the runs were compared. 
     In one embodiment, MS analysis of spent media reveals a secretome panel of 261 individual proteins (Table 1). In one example, secretome profiling using the secretome panel of 261 proteins (Table 1), as analyzed by MS, identifies the potential for developmental competence and implantation success of a human embryo. 
     In one embodiment, analysis of spent media via MS reveals a secretome panel of 37 individual proteins (Table 2). Secretome profiling via MS of 37 individual proteins (Table 2) from spent media of human embryos correlates with embryonic viability and euploidy. The term “euploidy” refers to having a chromosome number that is an exact multiple of the haploid number for a human embryo, namely 23 pairs of chromosomes. 
     In Tables 1 and 2 below, the entries for “Entry Name” and ““Accession Number” refer to identifiers for published sequence data that is stored in bioinformatic databases including the Uniprot Knowledgebase, Swiss-Prot and TrEMBL. The information is made freely available to the world and is coordinated by the Swiss Institute of Bioinformatics, which is centrally administered in Lausanne, Switzerland with offices in Bern, Geneva and Zurich. The sequence information represented by these identifiers together with the representative publications is hereby incorporated by reference to the same extent as though fully replicated herein. For those proteins having isoforms, the sequences for Table 1 include consensus sequences for the primary isoforms, while sequences for Table 2 include also all other isoforms available at the time of filing. 
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Proteins identified in spent media of human embryos. 
               
             
          
           
               
                 SEQ 
                   
                   
                 Protein 
                   
                   
               
               
                 ID 
                   
                   
                 Molecular 
                 Accession 
                   
               
               
                 NO. 
                 Protein Name 
                 Entry Name 
                 Weight 
                 Number 
                 Taxonomy 
               
               
                   
               
             
          
           
               
                 1 
                 Serum albumin 
                 ALBU_HUMAN 
                 69 
                 kDa 
                 P02768 
                 
                   Homo 
                   sapiens 
                 
               
               
                 2 
                 Serotransferrin 
                 TRFE_HUMAN 
                 77 
                 kDa 
                 P02787 
                 
                   Homo 
                   sapiens 
                 
               
               
                 3 
                 Haptoglobin 
                 HPT_HUMAN 
                 45 
                 kDa 
                 P00738 
                 
                   Homo 
                   sapiens 
                 
               
               
                 4 
                 Alpha-1-antitrypsin 
                 A1AT_HUMAN 
                 47 
                 kDa 
                 P01009 
                 
                   Homo 
                   sapiens 
                 
               
               
                 5 
                 Alpha-2-macroglobulin 
                 A2MG_HUMAN 
                 163 
                 kDa 
                 P01023 
                 
                   Homo 
                   sapiens 
                 
               
               
                 6 
                 Hemopexin 
                 HEMO_HUMAN 
                 52 
                 kDa 
                 P02790 
                 
                   Homo 
                   sapiens 
                 
               
               
                 7 
                 Apolipoprotein A-I 
                 APOA1_HUMAN 
                 31 
                 kDa 
                 P02647 
                 
                   Homo 
                   sapiens 
                 
               
               
                 8 
                 Ceruloplasmin 
                 CERU_HUMAN 
                 122 
                 kDa 
                 P00450 
                 
                   Homo 
                   sapiens 
                 
               
               
                 9 
                 Vitamin D-binding protein 
                 VTDB_HUMAN 
                 53 
                 kDa 
                 P02774 
                 
                   Homo 
                   sapiens 
                 
               
               
                 10 
                 Alpha-1-antichymotrypsin 
                 AACT_HUMAN 
                 48 
                 kDa 
                 P01011 
                 
                   Homo 
                   sapiens 
                 
               
               
                 11 
                 Plasma protease C1 inhibitor 
                 IC1_HUMAN 
                 55 
                 kDa 
                 P05155 
                 
                   Homo 
                   sapiens 
                 
               
               
                 12 
                 Alpha-1B-glycoprotein 
                 A1BG_HUMAN 
                 54 
                 kDa 
                 P04217 
                 
                   Homo 
                   sapiens 
                 
               
               
                 13 
                 Hemoglobin subunit beta 
                 HBB_HUMAN 
                 16 
                 kDa 
                 P68871 
                 
                   Homo 
                   sapiens 
                 
               
               
                 14 
                 Antithrombin-III 
                 ANT3_HUMAN 
                 53 
                 kDa 
                 P01008 
                 
                   Homo 
                   sapiens 
                 
               
               
                 15 
                 Afamin 
                 AFAM_HUMAN 
                 69 
                 kDa 
                 P43652 
                 
                   Homo 
                   sapiens 
                 
               
               
                 16 
                 Angiotensinogen 
                 ANGT_HUMAN 
                 53 
                 kDa 
                 P01019 
                 
                   Homo 
                   sapiens 
                 
               
               
                 17 
                 Transthyretin 
                 TTHY_HUMAN 
                 16 
                 kDa 
                 P02766 
                 
                   Homo 
                   sapiens 
                 
               
               
                 18 
                 Inter-alpha-trypsin inhibitor heavy 
                 ITIH4_HUMAN 
                 103 
                 kDa 
                 Q14624 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 chain H4 
                   
                   
                   
                   
                   
               
               
                 19 
                 Complement factor B 
                 CFAB_HUMAN 
                 86 
                 kDa 
                 P00751 
                 
                   Homo 
                   sapiens 
                 
               
               
                 20 
                 Hemoglobin subunit alpha 
                 HBA_HUMAN 
                 15 
                 kDa 
                 P69905 
                 
                   Homo 
                   sapiens 
                 
               
               
                 21 
                 Ig alpha-1 chain C region 
                 IGHA1_HUMAN 
                 38 
                 kDa 
                 P01876 
                 
                   Homo 
                   sapiens 
                 
               
               
                 22 
                 Ig gamma-1 chain C region 
                 IGHG1_HUMAN 
                 36 
                 kDa 
                 P01857 
                 
                   Homo 
                   sapiens 
                 
               
               
                 23 
                 Heparin cofactor 2 
                 HEP2_HUMAN 
                 57 
                 kDa 
                 P05546 
                 
                   Homo 
                   sapiens 
                 
               
               
                 24 
                 Ig gamma-2 chain C region 
                 IGHG2_HUMAN 
                 36 
                 kDa 
                 P01859 
                 
                   Homo 
                   sapiens 
                 
               
               
                 25 
                 Ig kappa chain C region 
                 KAC_HUMAN 
                 12 
                 kDa 
                 P01834 
                 
                   Homo 
                   sapiens 
                 
               
               
                 26 
                 N-acetylmuramoyl-L-alanine 
                 PGRP2_HUMAN 
                 62 
                 kDa 
                 Q96PD5 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 amidase 
                   
                   
                   
                   
                   
               
               
                 27 
                 Alpha-2-HS-glycoprotein 
                 FETUA_HUMAN 
                 39 
                 kDa 
                 P02765 
                 
                   Homo 
                   sapiens 
                 
               
               
                 28 
                 Kininogen-1 
                 KNG1_HUMAN 
                 72 
                 kDa 
                 P01042 
                 
                   Homo 
                   sapiens 
                 
               
               
                 29 
                 Zinc-alpha-2-glycoprotein 
                 ZA2G_HUMAN 
                 34 
                 kDa 
                 P25311 
                 
                   Homo 
                   sapiens 
                 
               
               
                 30 
                 Thyroxine-binding globulin 
                 THBG_HUMAN 
                 46 
                 kDa 
                 P05543 
                 
                   Homo 
                   sapiens 
                 
               
               
                 31 
                 Corticosteroid-binding globulin 
                 CBG_HUMAN 
                 45 
                 kDa 
                 P08185 
                 
                   Homo 
                   sapiens 
                 
               
               
                 32 
                 Attractin 
                 ATRN_HUMAN 
                 159 
                 kDa 
                 O75882 
                 
                   Homo 
                   sapiens 
                 
               
               
                 33 
                 Pregnancy zone protein 
                 PZP_HUMAN 
                 164 
                 kDa 
                 P20742 
                 
                   Homo 
                   sapiens 
                 
               
               
                 34 
                 Alpha-2-antiplasmin 
                 A2AP_HUMAN 
                 55 
                 kDa 
                 P08697 
                 
                   Homo 
                   sapiens 
                 
               
               
                 35 
                 Vitronectin 
                 VTNC_HUMAN 
                 54 
                 kDa 
                 P04004 
                 
                   Homo 
                   sapiens 
                 
               
               
                 36 
                 Ig lambda chain C regions 
                 LAC_HUMAN 
                 11 
                 kDa 
                 P01842 
                 
                   Homo 
                   sapiens 
                 
               
               
                 37 
                 Apolipoprotein A-II 
                 APOA2_HUMAN 
                 11 
                 kDa 
                 P02652 
                 
                   Homo 
                   sapiens 
                 
               
               
                 38 
                 Apolipoprotein C-III 
                 APOC3_HUMAN 
                 11 
                 kDa 
                 P02656 
                 
                   Homo 
                   sapiens 
                 
               
               
                 39 
                 Insulin-like growth factor-binding 
                 ALS_HUMAN 
                 66 
                 kDa 
                 P35858 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein complex acid labile chain 
                   
                   
                   
                   
                   
               
               
                 40 
                 Complement C3 
                 CO3_HUMAN 
                 187 
                 kDa 
                 P01024 
                 
                   Homo 
                   sapiens 
                 
               
               
                 41 
                 Complement C4-B 
                 CO4B_HUMAN 
                 193 
                 kDa 
                 P0C0L5 
                 
                   Homo 
                   sapiens 
                 
               
               
                 42 
                 Serum paraoxonase/arylesterase 1 
                 PON1_HUMAN 
                 40 
                 kDa 
                 P27169 
                 
                   Homo 
                   sapiens 
                 
               
               
                 43 
                 Plasma retinol-binding protein 
                 RETBP_HUMAN 
                 23 
                 kDa 
                 P02753 
                 
                   Homo 
                   sapiens 
                 
               
               
                 44 
                 Carboxypeptidase N subunit 2 
                 CPN2_HUMAN 
                 61 
                 kDa 
                 P22792 
                 
                   Homo 
                   sapiens 
                 
               
               
                 45 
                 Beta-2-glycoprotein 1 
                 APOH_HUMAN 
                 38 
                 kDa 
                 P02749 
                 
                   Homo 
                   sapiens 
                 
               
               
                 46 
                 Inter-alpha-trypsin inhibitor heavy 
                 ITIH2_HUMAN 
                 106 
                 kDa 
                 P19823 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 chain H2 
                   
                   
                   
                   
                   
               
               
                 47 
                 Clusterin 
                 CLUS_HUMAN 
                 52 
                 kDa 
                 P10909 
                 
                   Homo 
                   sapiens 
                 
               
               
                 48 
                 Protein AMBP 
                 AMBP_HUMAN 
                 39 
                 kDa 
                 P02760 
                 
                   Homo 
                   sapiens 
                 
               
               
                 49 
                 Ig heavy chain V-III region BRO 
                 HV305_HUMAN 
                 13 
                 kDa 
                 P01766 
                 
                   Homo 
                   sapiens 
                 
               
               
                 50 
                 Dynactin subunit 2 
                 DCTN2_HUMAN 
                 44 
                 kDa 
                 Q13561 
                 
                   Homo 
                   sapiens 
                 
               
               
                 51 
                 Inter-alpha-trypsin inhibitor heavy 
                 ITIH1_HUMAN 
                 101 
                 kDa 
                 P19827 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 chain H1 
                   
                   
                   
                   
                   
               
               
                 52 
                 Complement C2 
                 CO2_HUMAN 
                 83 
                 kDa 
                 P06681 
                 
                   Homo 
                   sapiens 
                 
               
               
                 53 
                 Serum amyloid P-component 
                 SAMP_HUMAN 
                 25 
                 kDa 
                 P02743 
                 
                   Homo 
                   sapiens 
                 
               
               
                 54 
                 Lamin-A/C 
                 LMNA_HUMAN 
                 74 
                 kDa 
                 P02545 
                 
                   Homo 
                   sapiens 
                 
               
               
                 55 
                 Phosphatidylinositol-glycan-specific 
                 PHLD1_HUMAN 
                 92 
                 kDa 
                 P80108 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 phospholipase D 
                   
                   
                   
                   
                   
               
               
                 56 
                 Alpha-1-acid glycoprotein 1 
                 A1AG1_HUMAN 
                 24 
                 kDa 
                 P02763 
                 
                   Homo 
                   sapiens 
                 
               
               
                 57 
                 Pigment epithelium-derived factor 
                 PEDF_HUMAN 
                 46 
                 kDa 
                 P36955 
                 
                   Homo 
                   sapiens 
                 
               
               
                 58 
                 Apolipoprotein C-I 
                 APOC1_HUMAN 
                 9 
                 kDa 
                 P02654 
                 
                   Homo 
                   sapiens 
                 
               
               
                 59 
                 Transcription intermediary factor 1- 
                 TIF1B_HUMAN 
                 89 
                 kDa 
                 Q13263 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 beta 
                   
                   
                   
                   
                   
               
               
                 60 
                 Leucine-rich alpha-2-glycoprotein 
                 A2GL_HUMAN 
                 38 
                 kDa 
                 P02750 
                 
                   Homo 
                   sapiens 
                 
               
               
                 61 
                 Lumican 
                 LUM_HUMAN 
                 38 
                 kDa 
                 P51884 
                 
                   Homo 
                   sapiens 
                 
               
               
                 62 
                 Ig kappa chain V-III region SIE 
                 KV302_HUMAN 
                 12 
                 kDa 
                 P01620 
                 
                   Homo 
                   sapiens 
                 
               
               
                 63 
                 Actin, cytoplasmic 1 
                 ACTB_HUMAN 
                 42 
                 kDa 
                 P60709 
                 
                   Homo 
                   sapiens 
                 
               
               
                 64 
                 Apolipoprotein E 
                 APOE_HUMAN 
                 36 
                 kDa 
                 P02649 
                 
                   Homo 
                   sapiens 
                 
               
               
                 65 
                 Kallistatin 
                 KAIN_HUMAN 
                 49 
                 kDa 
                 P29622 
                 
                   Homo 
                   sapiens 
                 
               
               
                 66 
                 Myosin-15 
                 MYH15_HUMAN 
                 225 
                 kDa 
                 Q9Y2K3 
                 
                   Homo 
                   sapiens 
                 
               
               
                 67 
                 Carboxypeptidase B2 
                 CBPB2_HUMAN 
                 48 
                 kDa 
                 Q96IY4 
                 
                   Homo 
                   sapiens 
                 
               
               
                 68 
                 Serum amyloid A-4 protein 
                 SAA4_HUMAN 
                 15 
                 kDa 
                 P35542 
                 
                   Homo 
                   sapiens 
                 
               
               
                 69 
                 Cholinesterase 
                 CHLE_HUMAN 
                 68 
                 kDa 
                 P06276 
                 
                   Homo 
                   sapiens 
                 
               
               
                 70 
                 Apolipoprotein D 
                 APOD_HUMAN 
                 21 
                 kDa 
                 P05090 
                 
                   Homo 
                   sapiens 
                 
               
               
                 71 
                 Tubulin beta-2C chain 
                 TBB2C_HUMAN 
                 50 
                 kDa 
                 P68371 
                 
                   Homo 
                   sapiens 
                 
               
               
                 72 
                 Haptoglobin-related protein 
                 HPTR_HUMAN 
                 39 
                 kDa 
                 P00739 
                 
                   Homo 
                   sapiens 
                 
               
               
                 73 
                 Hemoglobin subunit delta 
                 HBD_HUMAN 
                 16 
                 kDa 
                 P02042 
                 
                   Homo 
                   sapiens 
                 
               
               
                 74 
                 Ig kappa chain V-IV region Len 
                 KV402_HUMAN 
                 13 
                 kDa 
                 P01625 
                 
                   Homo 
                   sapiens 
                 
               
               
                 75 
                 Ig alpha-2 chain C region 
                 IGHA2_HUMAN 
                 37 
                 kDa 
                 P01877 
                 
                   Homo 
                   sapiens 
                 
               
               
                 76 
                 Proteoglycan 4 
                 PRG4_HUMAN 
                 151 
                 kDa 
                 Q92954 
                 
                   Homo 
                   sapiens 
                 
               
               
                 77 
                 Histone H2A type 1-D 
                 H2A1D_HUMAN 
                 14 
                 kDa 
                 P20671 
                 
                   Homo 
                   sapiens 
                 
               
               
                 78 
                 Biotinidase 
                 BTD_HUMAN 
                 61 
                 kDa 
                 P43251 
                 
                   Homo 
                   sapiens 
                 
               
               
                 79 
                 Carboxypeptidase N catalytic chain 
                 CBPN_HUMAN 
                 52 
                 kDa 
                 P15169 
                 
                   Homo 
                   sapiens 
                 
               
               
                 80 
                 Plasma kallikrein 
                 KLKB1_HUMAN 
                 71 
                 kDa 
                 P03952 
                 
                   Homo 
                   sapiens 
                 
               
               
                 81 
                 Ig kappa chain V-II region TEW 
                 KV204_HUMAN 
                 12 
                 kDa 
                 P01617 
                 
                   Homo 
                   sapiens 
                 
               
               
                 82 
                 Apolipoprotein C-II 
                 APOC2_HUMAN 
                 11 
                 kDa 
                 P02655 
                 
                   Homo 
                   sapiens 
                 
               
               
                 83 
                 DNA-dependent protein kinase 
                 PRKDC_HUMAN 
                 469 
                 kDa 
                 P78527 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 catalytic subunit 
                   
                   
                   
                   
                   
               
               
                 84 
                 Heterogeneous nuclear 
                 HNRL1_HUMAN 
                 96 
                 kDa 
                 Q9BUJ2 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein U-like protein 1 
                   
                   
                   
                   
                   
               
               
                 85 
                 Apolipoprotein B-100 
                 APOB_HUMAN 
                 516 
                 kDa 
                 P04114 
                 
                   Homo 
                   sapiens 
                 
               
               
                 86 
                 Heterogeneous nuclear 
                 ROA2_HUMAN 
                 37 
                 kDa 
                 P22626 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoproteins A2/B1 
                   
                   
                   
                   
                   
               
               
                 87 
                 Tubulin alpha-ubiquitous chain 
                 TBAK_HUMAN 
                 50 
                 kDa 
                 P68363 
                 
                   Homo 
                   sapiens 
                 
               
               
                 88 
                 Gelsolin 
                 GELS_HUMAN 
                 86 
                 kDa 
                 P06396 
                 
                   Homo 
                   sapiens 
                 
               
               
                 89 
                 Tetranectin 
                 TETN_HUMAN 
                 23 
                 kDa 
                 P05452 
                 
                   Homo 
                   sapiens 
                 
               
               
                 90 
                 Alpha-1-acid glycoprotein 2 
                 A1AG2_HUMAN 
                 24 
                 kDa 
                 P19652 
                 
                   Homo 
                   sapiens 
                 
               
               
                 91 
                 Ig heavy chain V-III region GAL 
                 HV320_HUMAN 
                 13 
                 kDa 
                 P01781 
                 
                   Homo 
                   sapiens 
                 
               
               
                 92 
                 IgGFc-binding protein 
                 FCGBP_HUMAN 
                 572 
                 kDa 
                 Q9Y6R7 
                 
                   Homo 
                   sapiens 
                 
               
               
                 93 
                 Lactotransferrin 
                 TRFL_HUMAN 
                 78 
                 kDa 
                 P02788 
                 
                   Homo 
                   sapiens 
                 
               
               
                 94 
                 Histone H4 
                 H4_HUMAN 
                 11 
                 kDa 
                 P62805 
                 
                   Homo 
                   sapiens 
                 
               
               
                 95 
                 Glutathione peroxidase 3 
                 GPX3_HUMAN 
                 26 
                 kDa 
                 P22352 
                 
                   Homo 
                   sapiens 
                 
               
               
                 96 
                 Histone H2B type 1-M 
                 H2B1M_HUMAN 
                 14 
                 kDa 
                 Q99879 
                 
                   Homo 
                   sapiens 
                 
               
               
                 97 
                 Apolipoprotein M 
                 APOM_HUMAN 
                 21 
                 kDa 
                 Q95445 
                 
                   Homo 
                   sapiens 
                 
               
               
                 98 
                 E3 SUMO-protein ligase RanBP2 
                 RBP2_HUMAN 
                 358 
                 kDa 
                 P49792 
                 
                   Homo 
                   sapiens 
                 
               
               
                 99 
                 Ig kappa chain V-I region EU 
                 KV106_HUMAN 
                 12 
                 kDa 
                 P01598 
                 
                   Homo 
                   sapiens 
                 
               
               
                 100 
                 Ig kappa chain V-I region Lay 
                 KV113_HUMAN 
                 12 
                 kDa 
                 P01605 
                 
                   Homo 
                   sapiens 
                 
               
               
                 101 
                 Dermcidin OS =  Homo   sapiens   
                 DCD_HUMAN 
                 11 
                 kDa 
                 P81605 
                 
                   Homo 
                   sapiens 
                 
               
               
                 102 
                 Leucine-rich repeats and 
                 LRIG2_HUMAN 
                 119 
                 kDa 
                 O94898 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 immunoglobulin-like domains 
                   
                   
                   
                   
                   
               
               
                   
                 protein 2 
                   
                   
                   
                   
                   
               
               
                 103 
                 Protein S100-A8 
                 S10A8_HUMAN 
                 11 
                 kDa 
                 P05109 
                 
                   Homo 
                   sapiens 
                 
               
               
                 104 
                 Glyceraldehyde-3-phosphate 
                 G3P_HUMAN 
                 36 
                 kDa 
                 P04406 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 dehydrogenase 
                   
                   
                   
                   
                   
               
               
                 105 
                 Myosin-9 
                 MYH9_HUMAN 
                 227 
                 kDa 
                 P35579 
                 
                   Homo 
                   sapiens 
                 
               
               
                 106 
                 Ig kappa chain V-III region VG 
                 KV309_HUMAN 
                 13 
                 kDa 
                 P04433 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 (Fragment) 
                   
                   
                   
                   
                   
               
               
                 107 
                 Monocyte differentiation antigen 
                 CD14_HUMAN 
                 40 
                 kDa 
                 P08571 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 CD14 
                   
                   
                   
                   
                   
               
               
                 108 
                 Heterogeneous nuclear 
                 HNRH1_HUMAN 
                 49 
                 kDa 
                 P31943 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein H 
                   
                   
                   
                   
                   
               
               
                 109 
                 ATP-dependent DNA helicase 2 
                 KU70_HUMAN 
                 70 
                 kDa 
                 P12956 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 subunit 1 
                   
                   
                   
                   
                   
               
               
                 110 
                 Protein TFG 
                 TFG_HUMAN 
                 43 
                 kDa 
                 Q92734 
                 
                   Homo 
                   sapiens 
                 
               
               
                 111 
                 Coagulation factor XII 
                 FA12_HUMAN 
                 68 
                 kDa 
                 P00748 
                 
                   Homo 
                   sapiens 
                 
               
               
                 112 
                 Filaggrin-2 
                 FILA2_HUMAN 
                 248 
                 kDa 
                 Q5D862 
                 
                   Homo 
                   sapiens 
                 
               
               
                 113 
                 Prothrombin 
                 THRB_HUMAN 
                 70 
                 kDa 
                 P00734 
                 
                   Homo 
                   sapiens 
                 
               
               
                 114 
                 Sex hormone-binding globulin 
                 SHBG_HUMAN 
                 44 
                 kDa 
                 P04278 
                 
                   Homo 
                   sapiens 
                 
               
               
                 115 
                 Heat shock cognate 71 kDa protein 
                 HSP7C_HUMAN 
                 71 
                 kDa 
                 P11142 
                 
                   Homo 
                   sapiens 
                 
               
               
                 116 
                 Histidine-rich glycoprotein 
                 HRG_HUMAN 
                 60 
                 kDa 
                 P04196 
                 
                   Homo 
                   sapiens 
                 
               
               
                 117 
                 Heterogeneous nuclear 
                 ROA3_HUMAN 
                 40 
                 kDa 
                 P51991 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein A3 
                   
                   
                   
                   
                   
               
               
                 118 
                 Ig kappa chain V-III region B6 
                 KV301_HUMAN 
                 12 
                 kDa 
                 P01619 
                 
                   Homo 
                   sapiens 
                 
               
               
                 119 
                 Testis-expressed sequence 15 
                 TEX15_HUMAN 
                 315 
                 kDa 
                 Q9BXT5 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 
                   
                   
                   
                   
                   
               
               
                 120 
                 Polyadenylate-binding protein 1 
                 PABP1_HUMAN 
                 71 
                 kDa 
                 P11940 
                 
                   Homo 
                   sapiens 
                 
               
               
                 121 
                 Lipopolysaccharide-binding protein 
                 LBP_HUMAN 
                 53 
                 kDa 
                 P18428 
                 
                   Homo 
                   sapiens 
                 
               
               
                 122 
                 Lamina-associated polypeptide 2, 
                 LAP2A_HUMAN 
                 75 
                 kDa 
                 P42166 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 isoform alpha 
                   
                   
                   
                   
                   
               
               
                 123 
                 Desmoplakin 
                 DESP_HUMAN 
                 332 
                 kDa 
                 P15924 
                 
                   Homo 
                   sapiens 
                 
               
               
                 124 
                 Protein S100-A9 
                 S10A9_HUMAN 
                 13 
                 kDa 
                 P06702 
                 
                   Homo 
                   sapiens 
                 
               
               
                 125 
                 Far upstream element-binding 
                 FUBP2_HUMAN 
                 73 
                 kDa 
                 Q92945 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 2 
                   
                   
                   
                   
                   
               
               
                 126 
                 Platelet glycoprotein lb alpha chain 
                 GP1BA_HUMAN 
                 69 
                 kDa 
                 P07359 
                 
                   Homo 
                   sapiens 
                 
               
               
                 127 
                 Zinc finger and SCAN domain- 
                 ZSC29_HUMAN 
                 97 
                 kDa 
                 Q8IWY8 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 containing protein 29 
                   
                   
                   
                   
                   
               
               
                 128 
                 Heterogeneous nuclear 
                 HNRH3_HUMAN 
                 37 
                 kDa 
                 P31942 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein H3 
                   
                   
                   
                   
                   
               
               
                 129 
                 Histone H3.3 
                 H33_HUMAN 
                 15 
                 kDa 
                 P84243 
                 
                   Homo 
                   sapiens 
                 
               
               
                 130 
                 Stress-70 protein, mitochondrial 
                 GRP75_HUMAN 
                 74 
                 kDa 
                 P38646 
                 
                   Homo 
                   sapiens 
                 
               
               
                 131 
                 Ig kappa chain V-I region AG 
                 KV101_HUMAN 
                 12 
                 kDa 
                 P01593 
                 
                   Homo 
                   sapiens 
                 
               
               
                 132 
                 Elongation factor 1-alpha 1 
                 EF1A1_HUMAN 
                 50 
                 kDa 
                 P68104 
                 
                   Homo 
                   sapiens 
                 
               
               
                 133 
                 RNA-binding protein FUS 
                 FUS_HUMAN 
                 53 
                 kDa 
                 P35637 
                 
                   Homo 
                   sapiens 
                 
               
               
                 134 
                 DNA repair and recombination 
                 RA54B_HUMAN 
                 103 
                 kDa 
                 Q9Y620 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein RAD54B 
                   
                   
                   
                   
                   
               
               
                 135 
                 Hornerin 
                 HORN_HUMAN 
                 282 
                 kDa 
                 Q86YZ3 
                 
                   Homo 
                   sapiens 
                 
               
               
                 136 
                 Tubulin beta chain 
                 TBB5_HUMAN 
                 50 
                 kDa 
                 P07437 
                 
                   Homo 
                   sapiens 
                 
               
               
                 137 
                 Heterogeneous nuclear 
                 HNRPC_HUMAN 
                 34 
                 kDa 
                 P07910 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoproteins C1/C2 
                   
                   
                   
                   
                   
               
               
                 138 
                 RecQ-mediated genome instability 
                 RMI1_HUMAN 
                 70 
                 kDa 
                 Q9H9A7 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 1 
                   
                   
                   
                   
                   
               
               
                 139 
                 Desmocollin-1 
                 DSC1_HUMAN 
                 100 
                 kDa 
                 Q08554 
                 
                   Homo 
                   sapiens 
                 
               
               
                 140 
                 Xaa-Pro dipeptidase 
                 PEPD_HUMAN 
                 55 
                 kDa 
                 P12955 
                 
                   Homo 
                   sapiens 
                 
               
               
                 141 
                 Junction plakoglobin 
                 PLAK_HUMAN 
                 82 
                 kDa 
                 P14923 
                 
                   Homo 
                   sapiens 
                 
               
               
                 142 
                 U3 small nucleolar RNA-associated 
                 UT14C_HUMAN 
                 87 
                 kDa 
                 Q5TAP6 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 14 homolog C 
                   
                   
                   
                   
                   
               
               
                 143 
                 Obscurin 
                 OBSCN_HUMAN 
                 868 
                 kDa 
                 Q5VST9 
                 
                   Homo 
                   sapiens 
                 
               
               
                 144 
                 Elongation factor Tu, mitochondrial 
                 EFTU_HUMAN 
                 50 
                 kDa 
                 P49411 
                 
                   Homo 
                   sapiens 
                 
               
               
                 145 
                 Heterogeneous nuclear 
                 HNRPF_HUMAN 
                 46 
                 kDa 
                 P52597 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein F 
                   
                   
                   
                   
                   
               
               
                 146 
                 Desmoglein-1 
                 DSG1_HUMAN 
                 114 
                 kDa 
                 Q02413 
                 
                   Homo 
                   sapiens 
                 
               
               
                 147 
                 Heterogeneous nuclear 
                 ROA1_HUMAN 
                 39 
                 kDa 
                 P09651 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein A1 
                   
                   
                   
                   
                   
               
               
                 148 
                 ELAV-like protein 1 
                 ELAV1_HUMAN 
                 36 
                 kDa 
                 Q15717 
                 
                   Homo 
                   sapiens 
                 
               
               
                 149 
                 Pyrroline-5-carboxylate reductase 1, 
                 P5CR1_HUMAN 
                 33 
                 kDa 
                 P32322 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 mitochondrial 
                   
                   
                   
                   
                   
               
               
                 150 
                 Ig lambda chain V-I region WAH 
                 LV106_HUMAN 
                 12 
                 kDa 
                 P04208 
                 
                   Homo 
                   sapiens 
                 
               
               
                 151 
                 Zinc finger and BTB domain- 
                 ZBT10_HUMAN 
                 92 
                 kDa 
                 Q96DT7 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 containing protein 10 
                   
                   
                   
                   
                   
               
               
                 152 
                 Cystatin-C 
                 CYTC_HUMAN 
                 16 
                 kDa 
                 P01034 
                 
                   Homo 
                   sapiens 
                 
               
               
                 153 
                 Major vault protein 
                 MVP_HUMAN 
                 99 
                 kDa 
                 Q14764 
                 
                   Homo 
                   sapiens 
                 
               
               
                 154 
                 Phosphatidylcholine-sterol 
                 LCAT_HUMAN 
                 50 
                 kDa 
                 P04180 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 acyltransferase 
                   
                   
                   
                   
                   
               
               
                 155 
                 Sulfhydryl oxidase 1 
                 QSCN6_HUMAN 
                 83 
                 kDa 
                 O00391 
                 
                   Homo 
                   sapiens 
                 
               
               
                 156 
                 Far upstream element-binding 
                 FUBP3_HUMAN 
                 62 
                 kDa 
                 Q96124 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 3 
                   
                   
                   
                   
                   
               
               
                 157 
                 Ig delta chain C region 
                 IGHD_HUMAN 
                 42 
                 kDa 
                 P01880 
                 
                   Homo 
                   sapiens 
                 
               
               
                 158 
                 Plasma serine protease inhibitor 
                 IPSP_HUMAN 
                 46 
                 kDa 
                 P05154 
                 
                   Homo 
                   sapiens 
                 
               
               
                 159 
                 Heterogeneous nuclear 
                 HNRPR_HUMAN 
                 71 
                 kDa 
                 O43390 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein R 
                   
                   
                   
                   
                   
               
               
                 160 
                 Hemoglobin subunit gamma-1 
                 HBG1_HUMAN 
                 16 
                 kDa 
                 P69891 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                   
                 (+1) 
                   
                   
                   
                   
               
               
                 161 
                 TAR DNA-binding protein 43 
                 TADBP_HUMAN 
                 45 
                 kDa 
                 Q13148 
                 
                   Homo 
                   sapiens 
                 
               
               
                 162 
                 Neural cell adhesion molecule Ll- 
                 CHL1_HUMAN 
                 135 
                 kDa 
                 O00533 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 like protein 
                   
                   
                   
                   
                   
               
               
                 163 
                 Bifunctional polynucleotide 
                 PNKP_HUMAN 
                 57 
                 kDa 
                 Q96160 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 phosphatase/kinase 
                   
                   
                   
                   
                   
               
               
                 164 
                 Fibrocystin 
                 PKHD1_HUMAN 
                 447 
                 kDa 
                 Q8TCZ9 
                 
                   Homo 
                   sapiens 
                 
               
               
                 165 
                 Heterogeneous nuclear 
                 ROAA_HUMAN 
                 36 
                 kDa 
                 Q99729 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein A/B 
                   
                   
                   
                   
                   
               
               
                 166 
                 Peroxiredoxin-1 
                 PRDX1_HUMAN 
                 22 
                 kDa 
                 Q06830 
                 
                   Homo 
                   sapiens 
                 
               
               
                 167 
                 40S ribosomal protein S16 
                 RS16_HUMAN 
                 16 
                 kDa 
                 P62249 
                 
                   Homo 
                   sapiens 
                 
               
               
                 168 
                 Heat shock 70 kDa protein 1 
                 HSP71_HUMAN 
                 70 
                 kDa 
                 P08107 
                 
                   Homo 
                   sapiens 
                 
               
               
                 169 
                 40S ribosomal protein S25 
                 RS25_HUMAN 
                 14 
                 kDa 
                 P62851 
                 
                   Homo 
                   sapiens 
                 
               
               
                 170 
                 Fetuin-B 
                 FETUB_HUMAN 
                 42 
                 kDa 
                 Q9UGM5 
                 
                   Homo 
                   sapiens 
                 
               
               
                 171 
                 Transferrin receptor protein 1 
                 TFR1_HUMAN 
                 85 
                 kDa 
                 P02786 
                 
                   Homo 
                   sapiens 
                 
               
               
                 172 
                 Myelin basic protein 
                 MBP_HUMAN 
                 33 
                 kDa 
                 P02686 
                 
                   Homo 
                   sapiens 
                 
               
               
                 173 
                 Aldo-keto reductase family 1 
                 AK1C1_HUMAN 
                 37 
                 kDa 
                 Q04828 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 member Cl 
                   
                   
                   
                   
                   
               
               
                 174 
                 Uncharacterized protein C19orf21 
                 CS021_HUMAN 
                 75 
                 kDa 
                 Q8IV12 
                 
                   Homo 
                   sapiens 
                 
               
               
                 175 
                 TATA-binding protein-associated 
                 RBP56_HUMAN 
                 62 
                 kDa 
                 Q92804 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 factor 2N 
                   
                   
                   
                   
                   
               
               
                 176 
                 ATP-dependent DNA helicase 2 
                 KU86_HUMAN 
                 83 
                 kDa 
                 P13010 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 subunit 2 
                   
                   
                   
                   
                   
               
               
                 177 
                 Sulfide:quinone oxidoreductase, 
                 SQRD_HUMAN 
                 50 
                 kDa 
                 Q9Y6N5 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 mitochondrial 
                   
                   
                   
                   
                   
               
               
                 178 
                 Interleukin enhancer-binding factor 
                 ILF2_HUMAN 
                 43 
                 kDa 
                 Q12905 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 2 
                   
                   
                   
                   
                   
               
               
                 179 
                 Prostaglandin F2-alpha receptor 
                 PF2R_HUMAN 
                 40 
                 kDa 
                 P43088 
                 
                   Homo 
                   sapiens 
                 
               
               
                 180 
                 Peptidase inhibitor 16 
                 PI16_HUMAN 
                 49 
                 kDa 
                 Q6UXB8 
                 
                   Homo 
                   sapiens 
                 
               
               
                 181 
                 Actin, alpha cardiac muscle 1 
                 ACTC_HUMAN 
                 42 
                 kDa 
                 P68032 
                 
                   Homo 
                   sapiens 
                 
               
               
                 182 
                 E3 SUMO-protein ligase RanBP2 
                 RBP2_HUMAN 
                 358 
                 kDa 
                 P49792 
                 
                   Homo 
                   sapiens 
                 
               
               
                 183 
                 Mitochondrial import inner 
                 TIM50_HUMAN 
                 40 
                 kDa 
                 Q3ZCQ8 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 membranetranslocase subunit 
                   
                   
                   
                   
                   
               
               
                   
                 TIM50 
                   
                   
                   
                   
                   
               
               
                 184 
                 Heterogeneous nuclear 
                 HNRPD_HUMAN 
                 38 
                 kDa 
                 Q14103 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein DO 
                   
                   
                   
                   
                   
               
               
                 185 
                 Interleukin enhancer-binding factor 
                 ILF3_HUMAN 
                 95 
                 kDa 
                 Q12906 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 3 
                   
                   
                   
                   
                   
               
               
                 186 
                 Heterogeneous nuclear 
                 HNRPG_HUMAN 
                 42 
                 kDa 
                 P38159 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein G 
                   
                   
                   
                   
                   
               
               
                 187 
                 40S ribosomal protein S4, X isoform 
                 RS4X_HUMAN 
                 30 
                 kDa 
                 P62701 
                 
                   Homo 
                   sapiens 
                 
               
               
                 188 
                 Caspase-14 
                 CASPE_HUMAN 
                 28 
                 kDa 
                 P31944 
                 
                   Homo 
                   sapiens 
                 
               
               
                 189 
                 Annexin A2 
                 ANXA2_HUMAN 
                 39 
                 kDa 
                 P07355 
                 
                   Homo 
                   sapiens 
                 
               
               
                 190 
                 Isocitrate dehydrogenase [NAD] 
                 IDH3A_HUMAN 
                 40 
                 kDa 
                 P50213 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 subunit alpha, mitochondrial 
                   
                   
                   
                   
                   
               
               
                 191 
                 Cysteine-rich secretory protein 3 
                 CRIS3_HUMAN 
                 28 
                 kDa 
                 P54108 
                 
                   Homo 
                   sapiens 
                 
               
               
                 192 
                 Myeloperoxidase 
                 PERM_HUMAN 
                 84 
                 kDa 
                 P05164 
                 
                   Homo 
                   sapiens 
                 
               
               
                 193 
                 Transformation/transcription 
                 TRRAP_HUMAN 
                 438 
                 kDa 
                 Q9Y4A5 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 domain-associated protein 
                   
                   
                   
                   
                   
               
               
                 194 
                 Isocitrate dehydrogenase [NAD] 
                 IDH3B_HUMAN 
                 42 
                 kDa 
                 O43837 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 subunit beta, mitochondrial 
                   
                   
                   
                   
                   
               
               
                 195 
                 Collagen alpha-2(V) chain 
                 CO5A2_HUMAN 
                 145 
                 kDa 
                 P05997 
                 
                   Homo 
                   sapiens 
                 
               
               
                 196 
                 Baculoviral IAP repeat-containing 
                 BIRC6_HUMAN 
                 528 
                 kDa 
                 Q9NR09 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 6 
                   
                   
                   
                   
                   
               
               
                 197 
                 Bleomycin hydrolase 
                 BLMH_HUMAN 
                 53 
                 kDa 
                 Q13867 
                 
                   Homo 
                   sapiens 
                 
               
               
                 198 
                 Poly(rC)-binding protein 1 
                 PCBP1_HUMAN 
                 37 
                 kDa 
                 Q15365 
                 
                   Homo 
                   sapiens 
                 
               
               
                 199 
                 Tubulin beta-3 chain 
                 TBB3_HUMAN 
                 50 
                 kDa 
                 Q13509 
                 
                   Homo 
                   sapiens 
                 
               
               
                 200 
                 60 kDa heat shock protein, 
                 CH60_HUMAN 
                 61 
                 kDa 
                 P10809 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 mitochondrial 
                   
                   
                   
                   
                   
               
               
                 201 
                 ATP synthase subunit beta, 
                 ATPB_HUMAN 
                 57 
                 kDa 
                 P06576 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 mitochondrial 
                   
                   
                   
                   
                   
               
               
                 202 
                 40S ribosomal protein S18 
                 RS18_HUMAN 
                 18 
                 kDa 
                 P62269 
                 
                   Homo 
                   sapiens 
                 
               
               
                 203 
                 DnaJ homolog subfamily A member 
                 DNJA1_HUMAN 
                 45 
                 kDa 
                 P31689 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 1 
                   
                   
                   
                   
                   
               
               
                 204 
                 RNA-binding motif, single-stranded- 
                 RBMS1_HUMAN 
                 45 
                 kDa 
                 P29558 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 interacting protein 1 
                   
                   
                   
                   
                   
               
               
                 205 
                 40S ribosomal protein S19 
                 RS19_HUMAN 
                 16 
                 kDa 
                 P39019 
                 
                   Homo 
                   sapiens 
                 
               
               
                 206 
                 Tubulin alpha-1 chain 
                 TBA1_HUMAN 
                 50 
                 kDa 
                 P68366 
                 
                   Homo 
                   sapiens 
                 
               
               
                 207 
                 Protein Hook homolog 1 
                 HOOK1_HUMAN 
                 85 
                 kDa 
                 Q9UJC3 
                 
                   Homo 
                   sapiens 
                 
               
               
                 208 
                 Brother of CDO 
                 BOC_HUMAN 
                 121 
                 kDa 
                 Q9BWV1 
                 
                   Homo 
                   sapiens 
                 
               
               
                 209 
                 Signal recognition particle 54 kDa 
                 SRP54_HUMAN 
                 56 
                 kDa 
                 P61011 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 
                   
                   
                   
                   
                   
               
               
                 210 
                 Trinucleotide repeat-containing 6B 
                 TNC6B_HUMAN 
                 194 
                 kDa 
                 Q9UP09 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 
                   
                   
                   
                   
                   
               
               
                 211 
                 Aldehyde dehydrogenase, dimeric 
                 AL3A1_HUMAN 
                 50 
                 kDa 
                 P30838 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 NADP-preferring 
                   
                   
                   
                   
                   
               
               
                 212 
                 40S ribosomal protein S3 
                 RS3_HUMAN 
                 27 
                 kDa 
                 P23396 
                 
                   Homo 
                   sapiens 
                 
               
               
                 213 
                 Fatty aldehyde dehydrogenase 
                 AL3A2_HUMAN 
                 55 
                 kDa 
                 P51648 
                 
                   Homo 
                   sapiens 
                 
               
               
                 214 
                 Electron transfer flavoprotein 
                 ETFA_HUMAN 
                 35 
                 kDa 
                 P13804 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 subunit alpha, mitochondrial 
                   
                   
                   
                   
                   
               
               
                 215 
                 RNA-binding protein EWS 
                 EWS_HUMAN 
                 68 
                 kDa 
                 Q01844 
                 
                   Homo 
                   sapiens 
                 
               
               
                 216 
                 Histone H2A.V 
                 H2AV_HUMAN 
                 14 
                 kDa 
                 Q71UI9 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                   
                 (+1) 
                   
                   
                   
                   
               
               
                 217 
                 Heterogeneous nuclear 
                 HNRPQ_HUMAN 
                 70 
                 kDa 
                 O60506 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 ribonucleoprotein Q 
                   
                   
                   
                   
                   
               
               
                 218 
                 Pyrroline-5-carboxylate reductase 
                 P5CR2_HUMAN 
                 34 
                 kDa 
                 Q96C36 
                 
                   Homo 
                   sapiens 
                 
               
               
                 219 
                 Prohibitin 
                 PHB_HUMAN 
                 30 
                 kDa 
                 P35232 
                 
                   Homo 
                   sapiens 
                 
               
               
                 220 
                 RuvB-like 1 
                 RUVB1_HUMAN 
                 50 
                 kDa 
                 Q9Y265 
                 
                   Homo 
                   sapiens 
                 
               
               
                 221 
                 Protein-glutamine gamma- 
                 TGM2_HUMAN 
                 77 
                 kDa 
                 P21980 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 glutamyltransferase 
                   
                   
                   
                   
                   
               
               
                 222 
                 Ubiquitin-associated protein 2 
                 UBAP2_HUMAN 
                 117 
                 kDa 
                 O5T6F2 
                 
                   Homo 
                   sapiens 
                 
               
               
                 223 
                 Latent-transforming growth factor 
                 LTB1L_HUMAN 
                 187 
                 kDa 
                 Q14766 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 beta-binding protein 1 
                   
                   
                   
                   
                   
               
               
                 224 
                 Thioredoxin domain-containing 
                 TXND2_HUMAN 
                 60 
                 kDa 
                 Q86VQ3 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 2 
                   
                   
                   
                   
                   
               
               
                 225 
                 Leucine-rich repeat-containing 
                 LRC17_HUMAN 
                 52 
                 kDa 
                 Q8N6Y2 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 17 
                   
                   
                   
                   
                   
               
               
                 226 
                 Protein FAM105B 
                 F105B_HUMAN 
                 40 
                 kDa 
                 Q96BN8 
                 
                   Homo 
                   sapiens 
                 
               
               
                 227 
                 Myosin-la 
                 MYO1A_HUMAN 
                 118 
                 kDa 
                 Q9UBC5 
                 
                   Homo 
                   sapiens 
                 
               
               
                 228 
                 Ras GTPase-activating-like protein 
                 IQGA2_HUMAN 
                 181 
                 kDa 
                 Q13576 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 IQGAP2 
                   
                   
                   
                   
                   
               
               
                 229 
                 26S proteasome non-ATPase 
                 PSMD1_HUMAN 
                 102 
                 kDa 
                 Q99460 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 regulatory subunit 1 
                   
                   
                   
                   
                   
               
               
                 230 
                 Actin, alpha skeletal muscle 
                 ACTS_HUMAN 
                 42 
                 kDa 
                 P68133 
                 
                   Homo 
                   sapiens 
                 
               
               
                 231 
                 Cleavage stimulation factor subunit 
                 CSTF3_HUMAN 
                 83 
                 kDa 
                 Q12996 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 3 
                   
                   
                   
                   
                   
               
               
                 232 
                 Clusterin 
                 CLUS_HUMAN 
                 52 
                 kDa 
                 P10909 
                 
                   Homo 
                   sapiens 
                 
               
               
                 233 
                 Fibrocystin-L 
                 PKHL1_HUMAN 
                 466 
                 kDa 
                 Q86W11 
                 
                   Homo 
                   sapiens 
                 
               
               
                 234 
                 InaD-like protein 
                 INADL_HUMAN 
                 196 
                 kDa 
                 Q8NI35 
                 
                   Homo 
                   sapiens 
                 
               
               
                 235 
                 Keratin, type I cuticular Ha1 
                 K1H1_HUMAN 
                 47 
                 kDa 
                 Q15323 
                 
                   Homo 
                   sapiens 
                 
               
               
                 236 
                 Keratin, type I cuticular Ha2 
                 K1H2_HUMAN 
                 50 
                 kDa 
                 Q14532 
                 
                   Homo 
                   sapiens 
                 
               
               
                 237 
                 Keratin, type I cuticular Ha6 
                 K1H6_HUMAN 
                 52 
                 kDa 
                 O76013 
                 
                   Homo 
                   sapiens 
                 
               
               
                 238 
                 Keratin, type I cytoskeletal 10 
                 K1C10_HUMAN 
                 59 
                 kDa 
                 P13645 
                 
                   Homo 
                   sapiens 
                 
               
               
                 239 
                 Keratin, type I cytoskeletal 14 
                 K1C14_HUMAN 
                 52 
                 kDa 
                 P02533 
                 
                   Homo 
                   sapiens 
                 
               
               
                 240 
                 Keratin, type I cytoskeletal 16 
                 K1C16_HUMAN 
                 51 
                 kDa 
                 P08779 
                 
                   Homo 
                   sapiens 
                 
               
               
                 241 
                 Keratin, type I cytoskeletal 18 
                 K1C18_HUMAN 
                 48 
                 kDa 
                 P05783 
                 
                   Homo 
                   sapiens 
                 
               
               
                 242 
                 Keratin, type I cytoskeletal 19 
                 K1C19_HUMAN 
                 44 
                 kDa 
                 P08727 
                 
                   Homo 
                   sapiens 
                 
               
               
                 243 
                 Keratin, type I cytoskeletal 9 
                 K1C9_HUMAN 
                 62 
                 kDa 
                 P35527 
                 
                   Homo 
                   sapiens 
                 
               
               
                 244 
                 Keratin, type II cuticular Hb1 
                 KR181_HUMAN 
                 55 
                 kDa 
                 Q14533 
                 
                   Homo 
                   sapiens 
                 
               
               
                 245 
                 Keratin, type II cuticular Hb5 
                 KR185_HUMAN 
                 56 
                 kDa 
                 P78386 
                 
                   Homo 
                   sapiens 
                 
               
               
                 246 
                 Keratin, type II cytoskeletal 1 
                 K2C1_HUMAN 
                 66 
                 kDa 
                 P04264 
                 
                   Homo 
                   sapiens 
                 
               
               
                 247 
                 Keratin, type II cytoskeletal 1b 
                 K2C1B_HUMAN 
                 62 
                 kDa 
                 Q7Z794 
                 
                   Homo 
                   sapiens 
                 
               
               
                 248 
                 Keratin, type II cytoskeletal 2 
                 K22E_HUMAN 
                 65 
                 kDa 
                 P35908 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 epidermal 
                   
                   
                   
                   
                   
               
               
                 249 
                 Keratin, type II cytoskeletal 4 
                 K2C4_HUMAN 
                 57 
                 kDa 
                 P19013 
                 
                   Homo 
                   sapiens 
                 
               
               
                 250 
                 Keratin, type II cytoskeletal 5 
                 K2C5_HUMAN 
                 62 
                 kDa 
                 P13647 
                 
                   Homo 
                   sapiens 
                 
               
               
                 251 
                 Keratin, type II cytoskeletal 6B 
                 K2C6B_HUMAN 
                 60 
                 kDa 
                 P04259 
                 
                   Homo 
                   sapiens 
                 
               
               
                 252 
                 Keratin, type II cytoskeletal 7 
                 K2C7_HUMAN 
                 51 
                 kDa 
                 P08729 
                 
                   Homo 
                   sapiens 
                 
               
               
                 253 
                 Keratin, type II cytoskeletal 8 
                 K2C8_HUMAN 
                 54 
                 kDa 
                 P05787 
                 
                   Homo 
                   sapiens 
                 
               
               
                 254 
                 Lymphoid-restricted membrane 
                 LRMP_HUMAN 
                 62 
                 kDa 
                 Q12912 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 protein 
                   
                   
                   
                   
                   
               
               
                 255 
                 Myosin light chain 1, skeletal muscle 
                 MLE1_HUMAN 
                 21 
                 kDa 
                 P05976 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 isoform 
                   
                   
                   
                   
                   
               
               
                 256 
                 Myosin-2 
                 MYH2_HUMAN 
                 223 
                 kDa 
                 Q9UKX2 
                 
                   Homo 
                   sapiens 
                 
               
               
                 257 
                 Protein Shroom3 
                 SHRM3_HUMAN 
                 216 
                 kDa 
                 Q8TF72 
                 
                   Homo 
                   sapiens 
                 
               
               
                 258 
                 Ras-related GTP-binding protein A 
                 RRAGA_HUMAN 
                 37 
                 kDa 
                 Q7L523 
                 
                   Homo 
                   sapiens 
                 
               
               
                 259 
                 Sushi, nidogen and EGF-like 
                 SNED1_HUMAN 
                 152 
                 kDa 
                 Q8TER0 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
                 domain-containing protein 1 
                   
                   
                   
                   
                   
               
               
                 260 
                 Retinol-binding protein 4 
                 RET4_HUMAN 
                 23 
                 kDa 
                 P02753 
                 
                   Homo 
                   sapiens 
                 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Proteins identified in spent media of human embryos.* 
               
             
          
           
               
                   
                   
                   
                 Protein 
                   
                 Day of 
                 Nature of 
               
               
                 SEQID 
                   
                   
                 Molecular 
                 Accession 
                 Embryo 
                 Viability 
               
               
                 NO. 
                 Protein Name 
                 Entry Name 
                 Weight 
                 Number 
                 Development 
                 Marker 
               
               
                   
               
               
                 261 
                 14-3-3 protein zeta/delta 
                 1433Z_HUMAN 
                  28 kDa 
                 P63104 
                 D1 
                   
               
               
                 262 
                 6-phosphofructokinase, muscle 
                 K6PF_HUMAN 
                  85 kDa 
                 P08237 
                 D1 
                   
               
               
                   
                 type, isoform 1 
                   
                   
                   
                   
                   
               
               
                 263 
                 6-phosphofructokinase, muscle 
                 K6PF_HUMAN 
                  82 kDa 
                 P08237-2 
                 D1 
                   
               
               
                   
                 type, isoform 1 
                   
                   
                   
                   
                   
               
               
                 264 
                 Adenylate kinase isoenzyme 1 
                 KAD1_HUMAN 
                  22 kDa 
                 P00568 
                 D1 
                   
               
               
                 265 
                 Alpha-actinin-2 
                 ACTN2_HUMAN 
                 104 kDa 
                 P35609 
                 D1 
                   
               
               
                 266 
                 Beta-enolase, isoform 1 
                 ENOB_HUMAN 
                  47 kDa 
                 P13929 
                 D1 
                   
               
               
                 267 
                 Beta-enolase, isoform 2 
                 ENOB_HUMAN 
                  44 kDa 
                 P13929-2 
                 D1 
                   
               
               
                 268 
                 Beta-enolase, isoform 3 
                 ENOB_HUMAN 
                  42 kDa 
                 P13929-3 
                 D1 
                   
               
               
                 269 
                 Creatine kinase M-type 
                 KCRM_HUMAN 
                  43 kDa 
                 P06732 
                 D1 
                   
               
               
                 270 
                 Fructose-bisphosphate aldolase A 
                 ALDOA_HUMAN 
                  39 kDa 
                 P04075 
                 D1 
                   
               
               
                 271 
                 Glycogen phosphorylase, muscle 
                 PYGM_HUMAN 
                  97 kDa 
                 P11217 
                 D1 
                   
               
               
                   
                 form 
                   
                   
                   
                   
                   
               
               
                 272 
                 L-lactate dehydrogenase A chain 
                 LDHA_HUMAN 
                  37 kDa 
                 P00338 
                 D1 
                   
               
               
                 273 
                 Myosin-1 
                 MYH1_HUMAN 
                 223 kDa 
                 P12882 
                 D1 
                   
               
               
                 274 
                 Myosin-2 
                 MYH2_HUMAN 
                 223 kDa 
                 Q9UKX2 
                 D1 
                   
               
               
                 275 
                 Myosin-3 
                 MYH3_HUMAN 
                 224 kDa 
                 P11055 
                 D1 
                   
               
               
                 276 
                 Myosin-4 
                 MYH4_HUMAN 
                 223 kDa 
                 Q9Y623 
                 D1 
                   
               
               
                 277 
                 Myosin-6 
                 MYH6_HUMAN 
                 224 kDa 
                 P13533 
                 D1 
                   
               
               
                 278 
                 Myosin-7 
                 MYH7_HUMAN 
                 223 kDa 
                 P12883 
                 D1 
                   
               
               
                 279 
                 Myosin-8 
                 MYH8_HUMAN 
                 223 kDa 
                 P13535 
                 D1 
                   
               
               
                 280 
                 Phosphoglucomutase-1, isoform 1 
                 PGM1_HUMAN 
                  61 kDa 
                 P36871 
                 D1 
                   
               
               
                 281 
                 Phosphoglucomutase-1, isoform 2 
                 PGM1_HUMAN 
                  64 kDa 
                 P36871-2 
                 D1 
                   
               
               
                 282 
                 Phosphoglycerate kinase 1 
                 PGK1_HUMAN 
                  45 kDa 
                 P00558 
                 D1 
                   
               
               
                 283 
                 Phosphoglycerate mutase 1 
                 PGAM1_HUMAN 
                  29 kDa 
                 P18669 
                 D1 
                   
               
               
                 284 
                 Pyruvate kinase isozyme M2 
                 KPYM_HUMAN 
                  58 kDa 
                 P14618 
                 D1 
                   
               
               
                 285 
                 Pyruvate kinase isozyme M1 
                 KPYM_HUMAN 
                  58 kDa 
                 P14618-2 
                 D1 
                   
               
               
                 286 
                 Sarcoplasmic/endoplasmic 
                 AT2A1_HUMAN 
                 110 kDa 
                 O14983 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 1, 
                   
                   
                   
                   
                   
               
               
                   
                 Isoform SERCA1B 
                   
                   
                   
                   
                   
               
               
                 287 
                 Sarcoplasmic/endoplasmic 
                 AT2A1_HUMAN 
                  109 kDa 
                 O14983-2 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 1, 
                   
                   
                   
                   
                   
               
               
                   
                 Isoform SERCA1A 
                   
                   
                   
                   
                   
               
               
                 288 
                 Sarcoplasmic/endoplasmic 
                 AT2A2_HUMAN 
                 115 kDa 
                 P16615 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 2, 
                   
                   
                   
                   
                   
               
               
                   
                 isoform 1 
                   
                   
                   
                   
                   
               
               
                 289 
                 Sarcoplasmic/endoplasmic 
                 AT2A2_HUMAN 
                 110 kDa 
                 P16615-2 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 2, 
                   
                   
                   
                   
                   
               
               
                   
                 isoform 2 
                   
                   
                   
                   
                   
               
               
                 290 
                 Sarcoplasmic/endoplasmic 
                 AT2A2_HUMAN 
                 110 kDa 
                 P16615-3 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 2, 
                   
                   
                   
                   
                   
               
               
                   
                 isoform 3 
                   
                   
                   
                   
                   
               
               
                 291 
                 Sarcoplasmic/endoplasmic 
                 AT2A2_HUMAN 
                 112 kDa 
                 P16615-4 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 2, 
                   
                   
                   
                   
                   
               
               
                   
                 isoform 4 
                   
                   
                   
                   
                   
               
               
                 292 
                 Sarcoplasmic/endoplasmic 
                 AT2A2_HUMAN 
                 110 kDa 
                 P16615-5 
                 D1 
                   
               
               
                   
                 reticulum calcium ATPase 2, 
                   
                   
                   
                   
                   
               
               
                   
                 isoform 5 
                   
                   
                   
                   
                   
               
               
                 293 
                 Triosephosphate isomerase, isoform 
                 TPIS_HUMAN 
                  27 kDa 
                 P60174 
                 D1 
                   
               
               
                   
                 1 
                   
                   
                   
                   
                   
               
               
                 294 
                 Triosephosphate isomerase, isoform 
                 TPIS_HUMAN 
                  27 kDa 
                 P60174-21 
                 D1 
                   
               
               
                   
                 2 
                   
                   
                   
                   
                   
               
               
                 295 
                 Tropomyosin alpha-1 chain, isoform 
                 TPM1_HUMAN 
                  33 kDa 
                 P09493 
                 D1 
                   
               
               
                   
                 1 
                   
                   
                   
                   
                   
               
               
                 296 
                 Tropomyosin alpha-1 chain, isoform 
                 TPM1_HUMAN 
                  27 kDa 
                 P09493-2 
                 D1 
                   
               
               
                   
                 2 
                   
                   
                   
                   
                   
               
               
                 297 
                 Tropomyosin alpha-1 chain, isoform 
                 TPM1_HUMAN 
                  33 kDa 
                 P09493-3 
                 D1 
                   
               
               
                   
                 3 
                   
                   
                   
                   
                   
               
               
                 298 
                 Tropomyosin alpha-1 chain, isoform 
                 TPM1_HUMAN 
                  33 kDa 
                 P09493-4 
                 D1 
                   
               
               
                   
                 4 
                   
                   
                   
                   
                   
               
               
                 299 
                 Tropomyosin alpha-1 chain, isoform 
                 TPM1_HUMAN 
                  28 kDa 
                 P09493-6 
                 D1 
                   
               
               
                   
                 5 
                   
                   
                   
                   
                   
               
               
                 300 
                 Tropomyosin alpha-3 chain, isoform 
                 TPM3_HUMAN 
                  33 kDa 
                 P06753 
                 D1 
                   
               
               
                   
                 1 
                   
                   
                   
                   
                   
               
               
                 301 
                 Tropomyosin alpha-3 chain, isoform 
                 TPM3_HUMAN 
                  29 kDa 
                 P06753-2 
                 D1 
                   
               
               
                   
                 2 
                   
                   
                   
                   
                   
               
               
                 302 
                 Tropomyosin alpha-3 chain, isoform  
                 TPM3_HUMAN 
                  29 kDa 
                 P06753-3 
                 D1 
                   
               
               
                   
                 3 
                   
                   
                   
                   
                   
               
               
                 303 
                 Collagen alpha-1(I) chain 
                 CO1A1_HUMAN 
                 139 kDa 
                 P02452 
                 D1/D3 
                   
               
               
                 304 
                 Cystatin-A 
                 CYTA_HUMAN 
                  11 kDa 
                 P01040 
                 D1/D3 
                   
               
               
                 305 
                 Filaggrin 
                 FILA_HUMAN 
                 435 kDa 
                 P20930 
                 D1/D3 
                   
               
               
                 306 
                 Keratinocyte proline-rich protein 
                 KPRP_HUMAN 
                  64 kDa 
                 Q5T749 
                 D1/D3 
                   
               
               
                 307 
                 Selenium-binding protein 1, isoform 
                 SBP1_HUMAN 
                  52 kDa 
                 Q13228 
                 D3 
                   
               
               
                   
                 1 
                   
                   
                   
                   
                   
               
               
                 308 
                 Selenium-binding protein 1, isoform 
                 SBP1_HUMAN 
                  45 kDa 
                 Q13228-2 
                 D3 
                   
               
               
                   
                 2 
                   
                   
                   
                   
                   
               
               
                 309 
                 Fatty acid-binding protein, epidermal 
                 FABP5_HUMAN 
                  15 kDa 
                 Q01469 
                 D1/D3/D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 310 
                 Galectin-7 
                 LEG7_HUMAN 
                  15 kDa 
                 P47929 
                 D1/D3/D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 311 
                 Ubiquitin 
                 UBIQ_HUMAN 
                 X kDa 
                 P02248 
                 D1/D3/D5 
                 Success 
               
               
                 312 
                 Calmodulin-like protein 5 
                 CALLS_HUMAN 
                  16 kDa 
                 Q9NZT1 
                 D1/D3/D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 313 
                 14-3-3 protein sigma 
                 1433S_HUMAN 
                  28 kDa 
                 P31947 
                 D1/D3/D5 
                 Success 
               
               
                 314 
                 Histone H2A type 1-A 
                 H2A1A_HUMAN 
                  14 kDa 
                 Q96QV6 
                 D1/D3/D5 
                   
               
               
                 315 
                 Histone H3.1t 
                 H311_HUMAN 
                  16 kDa 
                 Q16695 
                 D1/D3/D5 
                   
               
               
                 316 
                 Histone H1.3 
                 H13_HUMAN 
                  22 kDa 
                 P16402 
                 D3/D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 Aneuploidy 
               
               
                 317 
                 Lysozyme C 
                 LYSC_HUMAN 
                  17 kDa 
                 P61626 
                 D3/D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 318 
                 Plakophilin-1, isoform 2 
                 PKP1_HUMAN 
                  83 kDa 
                 Q13835 
                 D3/D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 319 
                 Plakophilin-1, isoform 1 
                 PKP1_HUMAN 
                  80 kDa 
                 Q13835-2 
                 D3/D5 
                 Success 
               
               
                 320 
                 Abhydrolase domain-containing 
                 AB12B_HUMAN 
                  41 kDa 
                 Q7Z5M8 
                 D3/D5 
                   
               
               
                   
                 protein 12B, isoform 1 
                   
                   
                   
                   
                   
               
               
                 321 
                 Abhydrolase domain-containing 
                 AB12B_HUMAN 
                  32 kDa 
                 Q7Z5M8-2 
                 D3/D5 
                   
               
               
                   
                 protein 12B, isoform 2 
                   
                   
                   
                   
                   
               
               
                 322 
                 Abhydrolase domain-containing 
                 AB12B_HUMAN 
                  29 kDa 
                 Q7Z5M8-3 
                 D3/D5 
                   
               
               
                   
                 protein 12B, isoform 3 
                   
                   
                   
                   
                   
               
               
                 323 
                 Abhydrolase domain-containing 
                 AB12B_HUMAN 
                  13 kDa 
                 Q7Z5M8-4 
                 D3/D5 
                   
               
               
                   
                 protein 12B, isoform 4 
                   
                   
                   
                   
                   
               
               
                 324 
                 Abhydrolase domain-containing 
                 AB12B_HUMAN 
                  25 kDa 
                 Q7Z5M8-5 
                 D3/D5 
                   
               
               
                   
                 protein 12B, isoform 5 
                   
                   
                   
                   
                   
               
               
                 325 
                 40S ribosomal protein SA 
                 RSSA_HUMAN 
                  33 kDa 
                 P08865 
                 D5 
                   
               
               
                 326 
                 Arginase-1, isoform 1 
                 ARGI1_HUMAN 
                  35 kDa 
                 P05089 
                 D5 
                   
               
               
                 327 
                 Arginase-1, isoform 2 
                 ARGI1_HUMAN 
                  36 kDa 
                 P05089-2 
                 D5 
                   
               
               
                 328 
                 Arginase-1, isoform 3 
                 ARGI1_HUMAN 
                  25 kDa 
                 P05089-3 
                 D5 
                   
               
               
                 329 
                 Beta-2-microglobulin 
                 B2MG_HUMAN 
                  14 kDa 
                 P61769 
                 D5 
                   
               
               
                 330 
                 Carbonic anhydrase 2 
                 CAH2_HUMAN 
                  29 kDa 
                 P00918 
                 D5 
                   
               
               
                 331 
                 Carboxypeptidase A4 
                 CBPA4_HUMAN 
                  47 kDa 
                 Q9UI42 
                 D5 
                   
               
               
                 332 
                 Catalase 
                 CATA_HUMAN 
                  60 kDa 
                 P04040 
                 D5 
                   
               
               
                 333 
                 Cathepsin D 
                 CATD_HUMAN 
                  45 kDa 
                 P07339 
                 D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 334 
                 CD44 antigen, isoform 1 
                 CD44_HUMAN 
                  81 kDa 
                 P16070 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 335 
                 CD44 antigen, isoform 2 
                 CD44_HUMAN 
                  3 kDa 
                 P16070-2 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 336 
                 CD44 antigen, isoform 3 
                 CD44_HUMAN 
                  78 kDa 
                 P16070-3 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 337 
                 CD44 antigen, isoform 4 
                 CD44_HUMAN 
                  77 kDa 
                 P16070-4 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 338 
                 CD44 antigen, isoform 5 
                 CD44_HUMAN 
                  81 kDa 
                 P16070-5 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 339 
                 CD44 antigen, isoform 6 
                 CD44_HUMAN 
                  77 kDa 
                 P16070-6 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 340 
                 CD44 antigen, isoform 7 
                 CD44_HUMAN 
                  78 kDa 
                 P16070-7 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 341 
                 CD44 antigen, isoform 8 
                 CD44_HUMAN 
                  74 kDa 
                 P16070-8 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 342 
                 CD44 antigen, isoform 9 
                 CD44_HUMAN 
                  74 kDa 
                 P16070-9 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 343 
                 CD44 antigen, isoform 10 
                 CD44_HUMAN 
                  53 kDa 
                 P16070-10 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 344 
                 CD44 antigen, isoform 11 
                 CD44_HUMAN 
                  47 kDa 
                 P16070-11 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 345 
                 CD44 antigen, isoform 12 
                 CD44_HUMAN 
                  39 kDa 
                 P16070-12 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 346 
                 CD44 antigen, isoform 13 
                 CD44_HUMAN 
                  46 kDa 
                 P16070-13 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 347 
                 CD44 antigen, isoform 14 
                 CD44_HUMAN 
                  43 kDa 
                 P16070-14 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 348 
                 CD44 antigen, isoform 15 
                 CD44_HUMAN 
                  32 kDa 
                 P16070-15 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 349 
                 CD44 antigen, isoform 16 
                 CD44_HUMAN 
                  73 kDa 
                 P16070-16 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 350 
                 CD44 antigen, isoform 17 
                 CD44_HUMAN 
                  76 kDa 
                 P16070-17 
                 D5 
                 Success 
               
               
                 351 
                 Creatine kinase B-type 
                 KCRB_HUMAN 
                  43 kDa 
                 P12277 
                 D5 
                   
               
               
                 352 
                 Dermokine, isoform 1 
                 DMKN_HUMAN 
                  47 kDa 
                 Q6E0U4 
                 D5 
                   
               
               
                 353 
                 Dermokine, isoform 2 
                 DMKN_HUMAN 
                  47 kDa 
                 Q6E0U4-2 
                 D5 
                   
               
               
                 354 
                 Dermokine, isoform 3 
                 DMKN_HUMAN 
                  37 kDa 
                 Q6E0U4-3 
                 D5 
                   
               
               
                 355 
                 Dermokine, isoform 4 
                 DMKN_HUMAN 
                  45 kDa 
                 Q6E0U4-4 
                 D5 
                   
               
               
                 356 
                 Dermokine, isoform 5 
                 DMKN_HUMAN 
                  38 kDa 
                 Q6E0U4-5 
                 D5 
                   
               
               
                 357 
                 Dermokine, isoform 6 
                 DMKN_HUMAN 
                  42 kDa 
                 Q6E0U4-6 
                 D5 
                   
               
               
                 358 
                 Dermokine, isoform 7 
                 DMKN_HUMAN 
                  35 kDa 
                 Q6E0U4-7 
                 D5 
                   
               
               
                 359 
                 Dermokine, isoform 8 
                 DMKN_HUMAN 
                  20 kDa 
                 Q6E0U4-8 
                 D5 
                   
               
               
                 360 
                 Dermokine, isoform 9 
                 DMKN_HUMAN 
                  19 kDa 
                 Q6E0U4-9 
                 D5 
                   
               
               
                 361 
                 Dermokine, isoform 10 
                 DMKN_HUMAN 
                  17 kDa 
                 Q6E0U4- 
                 D5 
                   
               
               
                   
                   
                   
                   
                 10 
                   
                   
               
               
                 362 
                 Dermokine, isoform 11 
                 DMKN_HUMAN 
                  15 kDa 
                 Q6E0U4- 
                 D5 
                   
               
               
                   
                   
                   
                   
                 11 
                   
                   
               
               
                 363 
                 Dermokine, isoform 12 
                 DMKN_HUMAN 
                  16 kDa 
                 Q6E0U4- 
                 D5 
                   
               
               
                   
                   
                   
                   
                 12 
                   
                   
               
               
                 364 
                 Dermokine, isoform 13 
                 DMKN_HUMAN 
                  14 kDa 
                 Q6E0U4- 
                 D5 
                   
               
               
                   
                   
                   
                   
                 13 
                   
                   
               
               
                 365 
                 Dermokine, isoform 14 
                 DMKN_HUMAN 
                  15 kDa 
                 Q6E0U4- 
                 D5 
                   
               
               
                   
                   
                   
                   
                 14 
                   
                   
               
               
                 366 
                 Dermokine, isoform 15 
                 DMKN_HUMAN 
                  10 kDa 
                 Q6E0U4- 
                 D5 
                   
               
               
                   
                   
                   
                   
                 15 
                   
                   
               
               
                 367 
                 Extracellular glycoprotein lacritin 
                 LACRT_HUMAN 
                  14 kDa 
                 Q9GZZ8 
                 D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 368 
                 Ferritin light chain (Ferritin L 
                 FRIL_HUMAN 
                  20 kDa 
                 P02792 
                 D5 
                 Success 
               
               
                   
                 subunit) 
                   
                   
                   
                   
                   
               
               
                 369 
                 Fibrinogen gamma chain, isoform 
                 FIBG_HUMAN 
                  52 kDa 
                 P02679 
                 D5 
                   
               
               
                   
                 gamma-B 
                   
                   
                   
                   
                   
               
               
                 370 
                 Fibrinogen gamma chain, isoform 
                 FIBG_HUMAN 
                  49 kDa 
                 P02679-2 
                 D5 
                 Increased 
               
               
                   
                 gamma-A 
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 371 
                 Gamma-enolase 
                 ENOG_HUMAN 
                  47 kDa 
                 P09104 
                 D5 
                 Success 
               
               
                 372 
                 Gamma-glutamylcyclotransferase, 
                 GGCT_HUMAN 
                  21 kDa 
                 O75223 
                 D5 
                   
               
               
                   
                 isoform 1 
                   
                   
                   
                   
                   
               
               
                 373 
                 Gamma-glutamylcyclotransferase, 
                 GGCT_HUMAN 
                  13 kDa 
                 O75223-2 
                 D5 
                 Increased 
               
               
                   
                 isoform 2 
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 374 
                 Heat shock protein beta-1 
                 HSPB1_HUMAN 
                  23 kDa 
                 P04792 
                 D5 
                 Success 
               
               
                 375 
                 Histidine ammonia-lyase 
                 HUTH_HUMAN 
                  73 kDa 
                 P42357 
                 D5 
                   
               
               
                 376 
                 Ig mu chain C region, isoform 1 
                 IGHM_HUMAN 
                  49 kDa 
                 P01871 
                 D5 
                   
               
               
                 377 
                 Ig mu chain C region, isoform 2 
                 IGHM_HUMAN 
                  52 kDa 
                 P01871-2 
                 D5 
                   
               
               
                 378 
                 Interleukin-1 family member 7 
                 IL1F7_HUMAN 
                 224 kDa 
                 Q9NZH6 
                 D5 
                   
               
               
                   
                 precursor, isoform B 
                   
                   
                   
                   
                   
               
               
                 379 
                 Interleukin-1 family member 7 
                 IL1F7_HUMAN 
                  22kDa 
                 Q9NZH6-2 
                 D5 
                   
               
               
                   
                 precursor, isoform A 
                   
                   
                   
                   
                   
               
               
                 380 
                 Interleukin-1 family member 7 
                 IL1F7_HUMAN 
                  20 kDa 
                 Q9NZH6-2 
                 D5 
                   
               
               
                   
                 precursor, isoform C 
                   
                   
                   
                   
                   
               
               
                 381 
                 Interleukin-1 family member 7 
                 IL1F7_HUMAN 
                  22 kDa 
                 Q9NZH6-2 
                 D5 
                   
               
               
                   
                 precursor, isoform D 
                   
                   
                   
                   
                   
               
               
                 382 
                 Interleukin-1 family member 7 
                 IL1F7_HUMAN 
                  17 kDa 
                 Q9NZH6-2 
                 D5 
                 Increased 
               
               
                   
                 precursor, isoform E 
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 Aneuploidy 
               
               
                 383 
                 Lipocalin-1 
                 LCN1_HUMAN 
                  19 kDa 
                 P31025 
                 D5 
                   
               
               
                 384 
                 Methionine synthase 
                 METH_HUMAN 
                 141 kDa 
                 099707 
                 D5 
                   
               
               
                 385 
                 Peroxiredoxin-2 
                 PRDX2_HUMAN 
                  22 kDa 
                 P32119 
                 D5 
                   
               
               
                 386 
                 Proprotein convertase 
                 PCSK9_HUMAN 
                  74 kDa 
                 Q8NBP7 
                 D5 
                   
               
               
                   
                 subtilisin/kexin type 9, isoform 1  
                   
                   
                   
                   
                   
               
               
                 387 
                 Proprotein convertase 
                 PCSK9_HUMAN 
                  21 kDa 
                 Q8NBP7-2 
                 D5 
                   
               
               
                   
                 subtilisin/kexin type 9, isoform 2  
                   
                   
                   
                   
                   
               
               
                 388 
                 Protein POF1B, isoform 1 
                 POF1B_HUMAN 
                  69 kDa 
                 Q8WVV4 
                 D5 
                   
               
               
                 389 
                 Protein POF1B, isoform 2 
                 POF1B_HUMAN 
                  68 kDa 
                 Q8WVV4-2 
                 D5 
                   
               
               
                 390 
                 Protein POF1B, isoform 3 
                 POF1B_HUMAN 
                  35 kDa 
                 Q8WVV4-3 
                 D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 391 
                 Protein S100-A7 
                 S10A7_HUMAN 
                  11 kDa 
                 P31151 
                 D5 
                 Success 
               
               
                 392 
                 Protein-glutamine gamma- 
                 TGM3_HUMAN 
                  77 kDa 
                 Q08188 
                 D5 
                   
               
               
                   
                 glutamyltransferase E 
                   
                   
                   
                   
                   
               
               
                 393 
                 Pyruvate kinase isozymes M1/M2, 
                 KPYM_HUMAN 
                  58 kDa 
                 P14618 
                 D5 
                   
               
               
                   
                 isoform M2 
                   
                   
                   
                   
                   
               
               
                 394 
                 Pyruvate kinase isozymes M1/M2, 
                 KPYM_HUMAN 
                  58 kDa 
                 P14618-2 
                 D5 
                   
               
               
                   
                 isoform M1 
                   
                   
                   
                   
                   
               
               
                 395 
                 Receptor-type tyrosine-protein 
                 PTPRG_HUMAN 
                 162 kDa 
                 P23470 
                 D5 
                   
               
               
                   
                 phosphatase gamma, isoform 1 
                   
                   
                   
                   
                   
               
               
                 396 
                 Receptor-type tyrosine-protein 
                 PTPRG_HUMAN 
                 159 kDa 
                 P23470-2 
                 D5 
                 Increased 
               
               
                   
                 phosphatase gamma, isoform 2 
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 397 
                 Serine protease inhibitor Kazal-type 
                 ISK5_HUMAN 
                 121 kDa 
                 Q9NQ38 
                 D5 
                 Success 
               
               
                   
                 5, isoform f1 
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 398 
                 Serine protease inhibitor Kazal-type 
                 ISK5_HUMAN 
                 104 kDa 
                 Q9NQ38 
                 D5 
                 Success 
               
               
                   
                 5, isoform short 
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 399 
                 Serine protease inhibitor Kazal-type 
                 ISK5_HUMAN 
                 124 kDa 
                 Q9NQ38 
                 D5 
                 Success 
               
               
                   
                 5, isoform long 
                   
                   
                   
                   
                   
               
               
                 400 
                 Serpin B3, isoform 1 
                 SPB3_HUMAN 
                  45 kDa 
                 P29508 
                 D5 
                   
               
               
                 401 
                 Serpin B3, isoform 2 
                 SPB3_HUMAN 
                  39 kDa 
                 P29508-2 
                 D5 
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 402 
                 Uteroglobin 
                 UTER_HUMAN 
                  10 kDa 
                 P11684 
                 D5 
                 Success 
               
               
                   
                   
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 403 
                 V-set and immunoglobulin domain- 
                 VSIG8_HUMAN 
                  44 kDa 
                 Q5VU13 
                 D5 
                 Success 
               
               
                   
                 containing protein 8, isoform 1 
                   
                   
                   
                   
                 Increased 
               
               
                   
                   
                   
                   
                   
                   
                 with 
               
               
                   
                   
                   
                   
                   
                   
                 implantation 
               
               
                 404 
                 V-set and immunoglobulin domain- 
                 VSIG8_HUMAN 
                  25 kDa 
                   
                 D5 
                 Success 
               
               
                   
                 containing protein 8, isoform 1 
               
               
                   
               
               
                 *All Proteins are from  Homo   Sapiens   
               
             
          
         
       
     
     Example 2 
     Secretome Profiling to Predict Implantation Potential of a Human Embryo 
     The following nonlimiting example teaches by way of illustration, not by limitation, the fabrication and employment of a customized immunoassay test kit for secretome profiling of a human embryo Immunoassay test kit fabrication occurs by modifying the well surfaces of a 96-well microtiter plate. Each separate well of the microtiter plate is incubated with an unlabelled capture antibody that recognizes one specific protein from the secretome panel. Custom capture antibodies are purchased from Rockland Immunochemicals, Inc. After incubation for 12 hours at 4° C., capture antibodies passively adsorb to the well surface. Subsequently, all wells of the 96-well plate are washed three times with a buffer comprising 1× phosphate buffer saline and 0.1% Tween-20 (PBST), blocked with 1% bovine serum albumin (BSA) for 1 hour at ambient temperature and washed three times with PBST. 
     Human cleavage-stage embryos are cultured in 10 μL drops of G1 supplemented with 2.5 mg/mL recombinant albumin under oil at 37° C., 6% CO 2 , 5% O 2  for 24 hours. The embryos are washed twice in G2 culture media and further cultured in 10 μL drops of G2 supplemented with 2.5 mg/mL recombinant albumin under oil at 37° C., 6% CO 2 , 5% O 2  for 48 hours with a fresh drop of G2 media added after 24 hours. Spent media samples of blastocysts are transferred into 0.65 mL Eppendorf tubes. Control groups comprise media cultured and collected under the same conditions but without embryos. 
     Secretome profiling proceeds using spent media culture in a sandwich ELISA format. The spent media culture is diluted into phosphate buffer saline (PBS) buffer and further distributed to separate wells of said customized microtiter plate. After incubation for one hour at room temperature, the samples are aspirated and all wells are washed four times with PBST buffer. Primary antibodies, each recognizing a distinct protein from the secretome panel, are distributed into wells that correspond to appropriate capture antibodies and permitted to incubate for one hour at room temperature. The wells are washed four times with PBST and incubated with a secondary antibody conjugated with an enzyme. The term “secondary antibody” refers to an antibody that binds to primary antibodies and may be conjugated with detection probes such as enzymes or fluorophores. In one embodiment, the secondary antibody contains the horseradish peroxidase (HRP) enzyme that converts the chromogenic substrate, 3,3′,5,5′-tetramethylbenzidene (TMB), into a blue product quantified at 655 nm on a spectrophotometer. In another embodiment, the HRP enzymatic reaction is stopped with a solution containing sulfuric acid and quantified at 450 nm. 
     In one embodiment, secretome profiling of a secretome panel of 261 individual proteins (Table 1), each selected for significant developmental competence and implantation potential, are monitored using said ELISA immunoassay test kit. In another embodiment, secretome profiling of a secretome panel comprising 37 proteins (Table 2) are monitored using said ELISA immunoassay test kit. Secretome profiling of the 37 individual proteins (Table 2) from spent media of human embryos correlates with embryonic viability and euploidy. 
     Changes may be made in the above methods and systems without departing from the scope hereof. It should be noted that the matter contained in the above description or shown in the accompanying drawings should be interpreted as illustrative and not in a limiting sense. The following claims are intended to cover all generic and specific features described herein, as well as all statements of the scope of the present method and system and reasonable variations thereof, which, as a matter of language, might be said to fall therebetween.