PATENT DOCUMENT

Abstract:
The present invention relates to nucleic acid primers and probes to detect one or more fungal and yeast species. More specifically the invention relates to the P2, P2A and P2B gene sequences (also known as 60S acidic ribosomal protein P2, RLA-2-ASPFU, Allergen ASP f8 or Afp2), the corresponding RNA, specific probes, primers and oligonucleotides related thereto and their use in diagnostic assays to detect and/or discriminate fungal and yeast species.

Full Description:
FIELD OF THE INVENTION  
       [0001]    The present invention relates to nucleic acid primers and probes to detect one or more fungal and yeast species. More specifically the invention relates to the P2, P2A and P2B gene sequences (also known as 60S acidic ribosomal protein P2, RLA-2-ASPFU, Allergen ASP f8 or Afp2), the corresponding RNA, specific probes, primers and oligonucleotides related thereto and their use in diagnostic assays to detect and/or discriminate fungal and yeast species. 
       BACKGROUND TO THE INVENTION  
       [0002]    Yeast and fungal infections represent a major cause of morbidity and mortality among immunocompromised patients. The number of immunocompromised patients at risk of yeast and fungal infection continues to increase each year, as does the spectrum of fungal and yeast agents causing disease. Mortality from fungal infections, particularly invasive fungal infections, is 30% or greater in certain risk groups. The array of available anti-fungal agents is growing; however, so too is the recognition of both intrinsic and emerging resistance to antifungal drugs. These factors are contributing to the increased need for cost containment in laboratory testing and have led to laboratory consolidation in testing procedures. 
         [0003]    Invasive fungal infections are on the increase. In 2003, it was estimated that there were 9 million at risk patients of which 1.2 million developed infection.  Candida  spp. and  Aspergillus  spp. now rank as the most prominent pathogens infecting immunosupressed patients. In particular, infections are common in the urinary tract, the respiratory system and the bloodstream, at the site of insertion of stents, catheters and orthopaedic joints. Approximately, 10% of the known  Candida  spp. have been implicated in human infection. Invasive candidiasis occurs when  candida  enters the bloodstream and it is estimated to occur at a frequency of 8/100,000 population in the US with a mortality rate of 40%.  Candida albicans  is the 4 th  most common cause of bloodstream infection. Aspergillosis usually begins as a pulmonary infection that can progress to a life-threatening invasive infection in some patients and has a mortality rate of greater than 90%. Emerging mycoses agents include  Fusarium, Scedosporium, Zygomycetes  and Trichosporon spp. (“ Stakeholder Insight: Invasive fungal infections ”, Datamonitor, January 2004). 
         [0004]    Immunocompromised patients including transplant and surgical patients, neonates, cancer patients, diabetics and those with HIV/AIDs are at high risk of developing invasive fungal infections (Datamonitor report: Stakeholder opinion—Invasive fungal infections, options outweigh replacements 2004). A large number of severe cases of sepsis are reported each year. Despite improvements in its medical management, sepsis still constitutes one of the greatest challenges in intensive care medicine. Microorganisms (bacteria, fungi and yeast) responsible for causing sepsis are traditionally detected in hospital laboratories with the aid of microbiological culture methods with poor sensitivity (25-82%), which are very time-consuming, generally taking from two to five days to complete, and up to eight days for the diagnosis of fungal infections. Definitive diagnosis of an infection caused by a yeast or fungus is usually based on either, the recovery and identification of a specific agent from clinical specimens or microscopic demonstration of fungi with distinct morphological features. However, there are numerous cases where these methods fail to provide conclusive proof as to the infecting agent. In these instances, the detection of specific host antibody responses can be used, although again this can be affected by the immune status of the patient. Time is critical in the detection and identification of bloodstream infections typically caused by bacteria and fungi. Effective treatment depends on finding the source of infection and making appropriate decisions about antibiotics or antifungals quickly and efficiently. Only after pathogens are correctly identified can targeted therapy using a specific antibiotic or anti-fungal begin. Many physicians would like to see the development of better in vitro amplification and direct detection diagnostic techniques for the early diagnosis of yeast and fungi (“Stakeholder Insight: Invasive fungal infections”, Datamonitor, January 2004). Recently Roche™ launched a real time PCR based assay (Septifast™), for the detection of bacterial, fungal and yeast DNA in clinical samples. Therefore, there is a clear need for the development of novel rapid diagnostic tests for clinically significant bacterial and fungal pathogens for bioanalysis applications in the clinical sector. This has led the current inventors to identify novel fungal and yeast nucleic acid targets for application in Nucleic Acid Diagnostics (NAD) tests. Fungal and yeast nucleic acid based diagnostics have focused heavily on the ribosomal RNA (rRNA) genes, RNA transcripts, and their associated DNA/RNA regions. The rRNA genes are highly conserved in all fungal species and they also contain divergent and distinctive intergenic transcribed spacer regions. Ribosomal rRNA comprises three genes: the large sub-unit gene (28S), the small sub-unit gene (18S) and the 5.8S gene. The 28S and 18S rRNA genes are separated by the 5.8S rRNA and two internal transcribed spacers (ITS 1 and ITS2). Because the ITS region contains a high number of sequence polymorphisms, numerous researchers have concentrated their efforts on these as targets (Atkins and Clark, 2004). rRNA genes are also multicopy genes with &gt;10 copies within the fungal genome. 
         [0005]    A number of groups are working on developing new assays for fungal and yeast infections. U.S. 2004044193 relates to, amongst a number of other aspects, the transcription factor CaTEC1 of  Candida albicans ; inhibitors thereof, and methods for the diagnosis and therapy of diseases which are connected with a  Candida  infection; and also diagnostic and pharmaceutical compositions which contain the nucleotide sequences, proteins, host cells and/or antibodies. WO0183824 relates to hybridization assay probes and accessory oligonucleotides for detecting ribosomal nucleic acids from  Candida albicans  and/or  Candida dubliniensis . U.S. Pat. No. 6,017,699 and U.S. Pat. No. 5,426,026 relate to a set of DNA primers, which can be used to amplify and speciate DNA from five medically important  Candida  species. U.S. Pat. No. 6,747,137 discloses sequences useful for diagnosis of  Candida  infections. EP 0422872 and U.S. Pat. No. 5,658,726 disclose probes based on 18S rRNA genes, and U.S. Pat. No. 5,958,693 discloses probes based on 28S rRNA, for diagnosis of a range of yeast and fungal species. U.S. Pat. No. 6,017,366 describes sequences based on chitin synthase gene for use in nucleic acid based diagnostics for a range of  Candida  species. It is clear though, that development of faster, more accurate diagnostic methods are required, particularly in light of the selection pressure caused by modern anti-microbial treatments which give rise to increased populations of resistant virulent strains with mutated genome sequences. Methods that enable early diagnosis of microbial causes of infection enable the selection of a specific narrow spectrum antibiotic or antifungal to treat the infection (Datamonitor report: Stakeholder opinion—Invasive fungal infections, options outweigh replacements 2004; Datamonitor report: Stakeholder Opinion—Sepsis, under reaction to an overreaction, 2006). 
         [0006]    Ribosomes are a two part organelle, composed of a large and a small subunit. The large subunit possesses a number of features which are conserved in all organisms for example the presence of a complex of a number of acidic proteins, the P proteins which form a stalk-like structure (Abramczyk et al., 2004 Tchórzewski et al., 2000; 2003). In the 60S ribosomal subunit of eukaryotes these acidic P proteins include, P0, P1 and P2 (Wool et al 1991). The complex formed by these P proteins is required for efficient translation. In yeasts, there are two isoforms of P1 (P1A and P1B) and P2 (P2A and P2B) which are encoded by four distinct single copy genes (Newton et al, 1990, Bailey-Serres et al., 1997). 
         [0007]    This invention relates to the use of the P2A, P2B genes in yeast and the P2 gene in fungi. There are currently 65 P2B gene sequences (approx. 360 bp) available in NCBI GenBank database including 3 P2B sequences for  Candida albicans  and one sequence of high homology for  C. glabrata . There are 329 P2 sequences available in NCBI GenBank database including annotated sequences for two  Aspergillus  species and presumptive P2 sequences for three species. Additional P2B sequences of representative species of  Candida  and P2 sequences of representative species of  Aspergillus  were generated by the inventors following the design of PCR primers to amplify P2B gene regions in  Candida  and P2 gene regions in  Aspergillus  spp. The published and newly generated P2B and P2 gene sequences for  Candida  and  Aspergillus  spp. respectively, were aligned and analysed using bioinformatics tools. PCR primers and species-specific DNA probes for selected species,  Candida glabrata  and  Aspergillus fumigatus  were designed and demonstrated for molecular species identification using real-time PCR. These examples demonstrate the potential of P2A/P2B and P2 genes for molecular identification of species of  Candida, Aspergillus  and other yeast and fungal species. 
       Definitions 
       [0008]    “Synthetic oligonucleotide” refers to molecules of nucleic acid polymers of 2 or more nucleotide bases that are not derived directly from genomic DNA or live organisms. The term synthetic oligonucleotide is intended to encompass DNA, RNA, and DNA/RNA hybrid molecules that have been manufactured chemically, or synthesized enzymatically in vitro. 
         [0009]    An “oligonucleotide” is a nucleotide polymer having two or more nucleotide subunits covalently joined together. Oligonucleotides are generally about 10 to about 100 nucleotides. The sugar groups of the nucleotide subunits may be ribose, deoxyribose, or modified derivatives thereof such as OMe. The nucleotide subunits may be joined by linkages such as phosphodiester linkages, modified linkages or by non-nucleotide moieties that do not prevent hybridization of the oligonucleotide to its complementary target nucleotide sequence. Modified linkages include those in which a standard phosphodiester linkage is replaced with a different linkage, such as a phosphorothioate linkage, a methylphosphonate linkage, or a neutral peptide linkage. Nitrogenous base analogs also may be components of oligonucleotides in accordance with the invention. A “target nucleic acid” is a nucleic acid comprising a target nucleic acid sequence. A “target nucleic acid sequence,” “target nucleotide sequence” or “target sequence” is a specific deoxyribonucleotide or ribonucleotide sequence that can be hybridized to a complementary oligonucleotide. 
         [0010]    An “oligonucleotide probe” is an oligonucleotide having a nucleotide sequence sufficiently complementary to its target nucleic acid sequence to be able to form a detectable hybrid probe:target duplex under high stringency hybridization conditions. An oligonucleotide probe is an isolated chemical species and may include additional nucleotides outside of the targeted region as long as such nucleotides do not prevent hybridization under high stringency hybridization conditions. Non-complementary sequences, such as promoter sequences, restriction endonuclease recognition sites, or sequences that confer a desired secondary or tertiary structure such as a catalytic active site can be used to facilitate detection using the invented probes. An oligonucleotide probe optionally may be labelled with a detectable moiety such as a radioisotope, a fluorescent moiety, a chemiluminescent, a nanoparticle moiety, an enzyme or a ligand, which can be used to detect or confirm probe hybridization to its target sequence. Oligonucleotide probes are preferred to be in the size range of from about 10 to about 100 nucleotides in length, although it is possible for probes to be as much as and above about 500 nucleotides in length, or below 10 nucleotides in length. 
         [0011]    A “hybrid” or a “duplex” is a complex formed between two single-stranded nucleic acid sequences by Watson-Crick base pairings or non-canonical base pairings between the complementary bases. “Hybridization” is the process by which two complementary strands of nucleic acid combine to form a double-stranded structure (“hybrid” or “duplex”). A “fungus” or “yeast” is meant any organism of the kingdom Fungi, and preferably, is directed towards any organism of the phylum Ascomycota. “Complementarity” is a property conferred by the base sequence of a single strand of DNA or RNA which may form a hybrid or double-stranded DNA:DNA, RNA:RNA or DNA:RNA through hydrogen bonding between Watson-Crick base pairs on the respective strands. Adenine (A) ordinarily complements thymine (T) or uracil (U), while guanine (G) ordinarily complements cytosine (C). 
         [0012]    The term “stringency” is used to describe the temperature, ionic strength and solvent composition existing during hybridization and the subsequent processing steps. Those skilled in the art will recognize that “stringency” conditions may be altered by varying those parameters either individually or together. Under high stringency conditions only highly complementary nucleic acid hybrids will form; hybrids without a sufficient degree of complementarity will not form. Accordingly, the stringency of the assay conditions determines the amount of complementarity needed between two nucleic acid strands forming a hybrid. Stringency conditions are chosen to maximize the difference in stability between the hybrid formed with the target and the non-target nucleic acid. With “high stringency” conditions, nucleic acid base pairing will occur only between nucleic acid fragments that have a high frequency of complementary base sequences (for example, hybridization under “high stringency” conditions, may occur between homologs with about 85-100% identity, preferably about 70-100% identity). With medium stringency conditions, nucleic acid base pairing will occur between nucleic acids with an intermediate frequency of complementary base sequences (for example, hybridization under “medium stringency” conditions may occur between homologs with about 50-70% identity). Thus, conditions of “weak” or “low” stringency are often required with nucleic acids that are derived from organisms that are genetically diverse, as the frequency of complementary sequences is usually less. 
         [0013]    ‘High stringency’ conditions are those equivalent to binding or hybridization at 42° C. in a solution consisting of 5×SSPE (43.8g/l NaCl, 6.9 g/l NaH 2 PO 4 H 2 O and 1.85 g/l EDTA, ph adjusted to 7.4 with NaOH), 0.5% SDS, 5× Denhardt&#39;s reagent and 100 μg/l denatured salmon sperm DNA followed by washing in a solution comprising 0.1×SSPE, 1.0% SDS at 42° C. when a probe of about 500 nucleotides in length is used. 
         [0014]    ‘Medium stringency’ conditions are those equivalent to binding or hybridization at 42° C. in a solution consisting of 5×SSPE (43.8 g/l NaCl, 6.9 g/l NaH 2 PO 4 H 2 O and 1.85 g/l EDTA, pH adjusted to 7.4 with NaOH), 0.5% SDS, 5×Denhardt&#39;s reagent and 100 μg/ml denatured salmon sperm DNA followed by washing in a solution comprising 1.0×SSPE, 1.0% SDS at 42° C., when a probe of about 500 nucleotides in length is used. 
         [0015]    ‘Low stringency’ conditions are those equivalent to binding or hybridization at 42° C. in a solution consisting of 5×SSPE (43.8 g/l NaCl, 6.9 g/l NaH 2 PO 4 H 2 O and 1.85 g/l EDTA, pH adjusted to 7.4 with NaOH), 0.1% SDS, 5×Denhardt&#39;s reagent [50× Denhardt&#39;s contains per 500 ml: 5 g Ficoll (Type 400, Pharamcia), 5 g BSA (Fraction V; Sigma)] and 100 μg/ml denatured salmon sperm DNA followed by washing in a solution comprising 5×SSPE, 0.1% SDS at 42° C., when a probe of about 500 nucleotides in length is used. 
         [0016]    In the context of nucleic acid in-vitro amplification based technologies, “stringency” is achieved by applying temperature conditions and ionic buffer conditions that are particular to that in-vitro amplification technology. For example, in the context of PCR and real-time PCR, “stringency” is achieved by applying specific temperatures and ionic buffer strength for hybridisation of the oligonucleotide primers and, with regards to real-time PCR hybridisation of the probe/s, to the target nucleic acid for in-vitro amplification of the target nucleic acid. 
         [0017]    One skilled in the art will understand that substantially corresponding probes of the invention can vary from the referred-to sequence and still hybridize to the same target nucleic acid sequence. This variation from the nucleic acid may be stated in terms of a percentage of identical bases within the sequence or the percentage of perfectly complementary bases between the probe and its target sequence. Probes of the present invention substantially correspond to a nucleic acid sequence if these percentages are from about 100% to about 80% or from 0 base mismatches in about 10 nucleotide target sequence to about 2 bases mismatched in an about 10 nucleotide target sequence. In preferred embodiments, the percentage is from about 100% to about 85%. In more preferred embodiments, this percentage is from about 90% to about 100%; in other preferred embodiments, this percentage is from about 95% to about 100% 
         [0018]    By “sufficiently complementary” or “substantially complementary” is meant nucleic acids having a sufficient amount of contiguous complementary nucleotides to form, under high stringency hybridization conditions, a hybrid that is stable for detection. The terms “identical” or percent “identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or higher identity over a specified region, when compared and aligned for maximum correspondence over a comparison window or designated region) as measured using a BLAST or BLAST 2.0 sequence comparison algorithms with default parameters described below, or by manual alignment and visual inspection (see, e.g., NCBI web site at ncbi.nlm.nih.gov/BLAST/or the like). Such sequences are then said to be “substantially identical.” This definition also refers to, or may be applied to, the compliment of a test sequence. The definition also includes sequences that have deletions and/or additions, as well as those that have substitutions. As described below, the preferred algorithms can account for gaps and the like. Preferably, identity exists over a region that is at least about 25 amino acids or nucleotides in length, or more preferably over a region that is 50-100 amino acids or nucleotides in length. 
         [0019]    For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Preferably, default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. 
         [0020]    A “comparison window,” as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith &amp; Waterman,  Adv. Appl. Math.  2:482 (1981), by the homology alignment algorithm of Needleman &amp; Wunsch,  J. Mol. Biol.  48:443 (1970), by the search for similarity method of Pearson &amp; Lipman,  Proc. Nat&#39;l. Acad. Sci. USA  85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by manual alignment and visual inspection (see, e.g.,  Current Protocols in Molecular Biology  (Ausubel et al., eds. 1987-2005, Wiley Interscience)). A preferred example of algorithm that is suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al.,  Nuc. Acids Res.  25:3389-3402 (1977) and Altschul et al.,  J. Mol. Biol.  215:403-410 (1990), respectively. BLAST and BLAST 2.0 are used, with the parameters described herein, to determine percent sequence identity for the nucleic acids and proteins of the invention. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always &gt;0) and N (penalty score for mismatching residues; always &lt;0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, M=5, N=−4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff &amp; Henikoff,  Proc. Natl. Acad. Sci. USA  89:10915 (1989)) alignments (B) of 50, expectation (E) of 10, M=5, N=−4, and a comparison of both strands. 
         [0021]    “Nucleic acid” refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form, and complements thereof. The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, peptide-nucleic acids (PNAs). 
         [0022]    By “nucleic acid hybrid” or “probe:target duplex” is meant a structure that is a double-stranded, hydrogen-bonded structure, preferably about 10 to about 100 nucleotides in length, more preferably 14 to 50 nucleotides in length, although this will depend to an extent on the overall length of the oligonucleotide probe. The structure is sufficiently stable to be detected by means such as chemiluminescent or fluorescent light detection, autoradiography, electrochemical analysis or gel electrophoresis. Such hybrids include RNA:RNA, RNA:DNA, or DNA:DNA duplex molecules. 
         [0023]    “RNA and DNA equivalents” refer to RNA and DNA molecules having the same complementary base pair hybridization properties. RNA and DNA equivalents have different sugar groups (i.e., ribose versus deoxyribose), and may differ by the presence of uracil in RNA and thymine in DNA. The difference between RNA and DNA equivalents do not contribute to differences in substantially corresponding nucleic acid sequences because the equivalents have the same degree of complementarity to a particular sequence. 
         [0024]    By “preferentially hybridize” is meant that under high stringency hybridization conditions oligonucleotide probes can hybridize their target nucleic acids to form stable probe:target hybrids (thereby indicating the presence of the target nucleic acids) without forming stable probe:non-target hybrids (that would indicate the presence of non-target nucleic acids from other organisms). Thus, the probe hybridizes to target nucleic acid to a sufficiently greater extent than to non-target nucleic acid to enable one skilled in the art to accurately detect the presence of (for example  Candida ) and distinguish these species from other organisms. Preferential hybridization can be measured using techniques known in the art and described herein. 
         [0025]    By “theranostics” is meant the use of diagnostic testing to diagnose the disease, choose the correct treatment regime and monitor the patient response to therapy. The theranostics of the invention may be based on the use of an NAD assay of this invention on samples, swabs or specimens collected from the patient. 
       OBJECT OF THE INVENTION  
       [0026]    It is an object of the current invention to provide sequences and/or diagnostic assays to detect and identify one or more yeast and fungal species. The current inventors have made use of the P2, P2A and P2B gene sequences to design primers that are specific to  Candida  P2B genes and to  Aspergillus  P2 genes. Such primers not only allow the detection of yeast and fungal species but also allow distinction between  Candida  and  Aspergillus  spp. This has an advantage over the prior art in that if one wants to identify a fungal pathogen in a sample, which contains  Candida  as a commensal, the approach of using universal primers may not be successful. There is a strong possibility that the  Candida  will out-compete the fungal pathogen in the amplification process and will be preferentially amplified, resulting in failure to detect the disease-causing pathogen. The current invention further provides for primers and probes that allow discrimination between different  Candida  species and among different  Aspergillus  species. 
       SUMMARY OF THE INVENTION 
       [0027]    The present invention provides for a diagnostic kit for detection and identification of yeast and/or fungal species, comprising an oligonucleotide probe capable of binding to at least a portion of the P2, P2A or P2B gene or its corresponding mRNA. The oligonucleotide probe may have a sequence substantially homologous to or substantially complementary to a portion of the P2, P2A or P2B gene or its corresponding mRNA. It will thus be capable of binding or hybridizing with a complementary DNA or RNA molecule. The P2 gene may be a fungal P2 gene. The P2A gene may be a yeast gene. The P2B gene may be yeast P2B gene. The nucleic acid molecule may be synthetic. The oligonucleotide probe may have a sequence of SEQ ID NO 9, 10, 91-94, 105-109, or a sequence substantially homologous to or substantially complementary to those sequences, which can also act as a probe for the P2, P2A or P2B genes. 
         [0028]    The kit may comprise more than one such probe. In particular the kit may comprise a plurality of such probes. In addition, the kit may comprise additional probes for other organisms, such as, for example, bacterial species or viruses. 
         [0029]    The identified sequences are suitable not only for in vitro DNA/RNA amplification based detection systems but also for signal amplification based detection systems. Furthermore, the sequences of the invention identified as suitable targets provide the advantages of having significant intragenic sequence heterogeneity in some regions, which is advantageous and enables aspects of the invention to be directed towards group or species-specific targets, and also having significant sequence homogeneity in some regions, which enables aspects of the invention to be directed towards genus-specific yeast and fungal primers and probes for use in direct nucleic acid detection technologies, signal amplification nucleic acid detection technologies, and nucleic acid in vitro amplification technologies for yeast and fungal diagnostics. The P2, P2A and P2B sequences allow for multi-test capability and automation in diagnostic assays. One of the advantages of the sequences of the present invention is that the intragenic P2, P2A and P2B nucleotide sequences diversity between closely related yeast or fungal species enables specific primers and probes for use in diagnostics assays for the detection of yeast and fungi to be designed. The P2, P2A and P2B nucleotide sequences, both DNA and RNA can be used with direct detection, signal amplification detection and in vitro amplification technologies in diagnostics assays. The P2, P2A and P2B sequences allow for multi-test capability and automation in diagnostic assays. 
         [0030]    The kit may further comprise a primer for amplification of at least a portion of the P2 and/or P2A and/or P2B genes. Suitably, the kit comprises a forward and a reverse primer for a portion of the P2, P2A and/or P2B gene. 
         [0031]    The portion of the P2A gene may be equivalent to a portion of the region of the gene from by position 114 to by position 439 of  C. albicans  (AF317661.1). 
         [0032]    The portion of the P2B gene may be equivalent to a portion of the region of the gene from base pair position 1 to position 335 in  C. albicans  (XM — 718047.1, XM — 717900.1) or position 8 to base pair position 359 of the P2B gene in  C. albicans  (AF317662.1).  C. albicans.    
         [0033]    Particularly preferred, are kits comprising, a probe for a portion of the P2A  C. albicans  gene and/or a probe for a portion of the region of the gene equivalent to base pair position 114 to by position 439 of  C. albicans  (AF317661.1). 
         [0034]    Particularly preferred, are kits comprising, a probe for a portion of the P2B  C. albicans  gene and/or a probe for a portion of the region of the gene equivalent to base pair position 1 to position 359. Also preferred are probes for a portion of the region of the gene equivalent to base pair position 1 to position 335 in  C. albicans  (XM — 718047.1, XM_717900.1) or position 8 to by position 359 of the P2B gene in  C. albicans  (AF317662.1). Equivalent base pair positioning may be found in other organisms, but not necessarily in the same position. 
         [0035]    The portion of the P2B gene may be equivalent to a portion of the region of the gene from base pair 24 to by position 158 in  C. glabrata  (XM — 444905.1). Particularly preferred, are kits comprising, a probe for a portion of the P2B  C. glabrata  gene and/or a probe for a portion of the region of the gene equivalent to base pair position 24 to base pair position 158 in  C. glabrata . Sequences equivalent to base pair position 24 to base pair position 158 can be found in other organisms, but not necessarily in the same position. The kit may also comprise additional primers or probes. 
         [0036]    The portion of the P2 gene may be equivalent to a portion of the region of the gene from base pair positions 1 to 326 in  A. fumigatus . Particularly preferred, are kits comprising, a probe for a portion of the P2  A. fumigatus  gene and/or a probe for a portion of the region of the gene equivalent to base pair position 1 to base pair position 326 in  A. fumigatus . Equivalent sequences to base pair position 1 to base pair position 326 can be found in other organisms, but not necessarily in the same position. The kit may also comprise additional primers or probes. 
         [0037]    The primer may have a sequence selected from the group SEQ ID NO 1-8, 85-90, 95-104, and a sequence substantially homologous to or substantially complementary to those sequences, which can also act as a primer for the P2, P2A and P2B genes. The primers may also be a primer which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by the primers SEQ ID NO 1-8, 85-90, 95-104. 
         [0038]    The kit may comprise at least one forward in vitro amplification primer and at least one reverse in vitro amplification primer, the forward amplification primer having a sequence selected from the group consisting of SEQ ID NO 1, 3, 7; 85-88, 95-99, and a sequence being substantially homologous or complementary thereto which can also act as a forward amplification primer for the P2A, P2B or P2 genes, and the reverse amplification primer having a sequence selected from the group consisting of SEQ ID NO 2, 4, 5, 6, 8, 89, 90, 100-104, and a sequence being substantially homologous or complementary thereto which can also act as a reverse amplification primer for the P2A, P2B or P2 genes 
         [0039]    The diagnostic kit may be based on direct nucleic acid detection technologies, signal amplification nucleic acid detection technologies, and nucleic acid in vitro amplification technologies is selected from one or more of Polymerase Chain Reaction (PCR), Ligase Chain Reaction (LCR), Nucleic Acids Sequence Based Amplification (NASBA), Strand Displacement Amplification (SDA), Transcription Mediated Amplification (TMA), Branched DNA technology (bDNA) and Rolling Circle Amplification Technology (RCAT)), or other in vitro enzymatic amplification technologies. 
         [0040]    The invention also provides a nucleic acid molecule selected from the group consisting of SEQ ID NO.1 to SEQ ID NO. 110 and sequences substantially homologous thereto, or substantially complementary to a portion thereof and having a function in diagnostics based on the P2 and/or P2A and P2B genes. The nucleic acid molecule may comprise an oligonucleotide having a sequence substantially homologous to or substantially complementary to a portion of a nucleic acid molecule of SEQ ID NO.1 to SEQ ID NO. 110. The invention also provides a method of detecting a target organism in a test sample comprising the steps of: 
         [0041]    (i) Mixing the test sample with at least one oligonucleotide probe as defined above under appropriate conditions; and 
         [0042]    (ii) hybridizing under high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex; and 
         [0043]    (iii) determining whether a probe:target duplex is present; the presence of the duplex positively identifying the presence of the target organism in the test sample. 
         [0044]    The nucleic acid molecule and kits of the present invention may be used in a diagnostic assay to detect the presence of one or more yeast and/or fungal species, to measure yeast and/or fungal titres in a patient or in a method of assessing the efficacy of a treatment regime designed to reduce yeast and/or fungal titre in a patient or to measure yeast and/or fungal contamination in an environment. The environment may be a hospital, or it may be a food sample, an environmental sample e.g. water, an industrial sample such as an in-process sample or an end product requiring bioburden or quality assessment. The kits and the nucleic acid molecule of the invention may be used in the identification and/or characterization of one or more disruptive agents that can be used to disrupt the P2A, P2B or P2 gene function. The disruptive agent may be selected from the group consisting of antisense RNA, PNA, and siRNA. 
         [0045]    In some embodiments of the invention, a nucleic acid molecule comprising a species-specific probe can be used to discriminate between species of the same genus. The oligonucleotides of the invention may be provided in a composition for detecting the nucleic acids of yeast and fungal target organisms. Such a composition may also comprise buffers, enzymes, detergents, salts and so on, as appropriate to the intended use of the compositions. It is also envisioned that the compositions, kits and methods of the invention, while described herein as comprising at least one synthetic oligonucleotide, may also comprise natural oligonucleotides with substantially the same sequences as the synthetic nucleotide fragments in place of, or alongside synthetic oligonucleotides. 
         [0046]    The invention also provides for an in vitro amplification diagnostic kit for a target yeast and/or fungal organism comprising at least one forward in vitro amplification primer and at least one reverse in vitro amplification primer, the forward amplification primer being selected from the group consisting of one or more of a sequence being substantially homologous or complementary thereto which can also act as a forward amplification primer, and the reverse amplification primer being selected from the group consisting of one or more of or a sequence being substantially homologous or complementary thereto which can also act as a reverse amplification primer. 
         [0047]    The invention also provides for a diagnostic kit for detecting the presence of candidate yeast and/or fungal species, comprising one or more DNA probes comprising a sequence substantially complementary to, or substantially homologous to the sequence of the P2A, P2B or P2 gene of the candidate yeast and/or fungal species. The present invention also provides for one or more synthetic oligonucleotides having a nucleotide sequence substantially homologous to or substantially complementary to one or more of the group consisting of the P2A, P2B gene or mRNA transcripts thereof, the yeast and or fungal P2 gene or mRNA transcript thereof, the yeast P2B gene or mRNA transcript thereof, one or more of SEQ ID NO 1-SEQ ID NO 110. 
         [0048]    The nucleotide may comprise DNA. The nucleotide may comprise RNA. The nucleotide may comprise a mixture of DNA, RNA and PNA. The nucleotide may comprise synthetic nucleotides. The sequences of the invention (and the sequences relating to the methods, kits compositions and assays of the invention) may be selected to be substantially homologous to a portion of the coding region of the P2, P2A or P2B genes. The gene may be a gene from a target yeast or fungal organism. The sequences of the invention are preferably sufficient so as to be able form a probe:target duplex to the portion of the sequence. 
         [0049]    The invention also provides for a diagnostic kit for a target yeast or fungal organism comprising an oligonucleotide probe substantially homologous to or substantially complementary to an oligonucleotide of the invention (which may be synthetic). It will be appreciated that sequences suitable for use as in vitro amplification primers may also be suitable for use as oligonucleotide probes: while it is preferable that amplification primers may have a complementary portion of between about 15 nucleotides and about 30 nucleotides (more preferably about 15-about 23, most preferably about 20 to about 23), oligonucleotide probes of the invention may be any suitable length. The skilled person will appreciate that different hybridization and or annealing conditions will be required depending on the length, nature &amp; structure (eg. Hybridization probe pairs for LightCycler, Taqman 5′ exonuclease probes, hairpin loop structures etc. and sequence of the oligonucleotide probe selected. 
         [0050]    Kits and assays of the invention may also be provided wherein the oligonucleotide probe is immobilized on a surface. Such a surface may be a bead, a membrane, a column, dipstick, a nanoparticle, the interior surface of a reaction chamber such as the well of a diagnostic plate or inside of a reaction tube, capillary or vessel or the like. 
         [0051]    The target yeast or fungal organism may be selected from the group consisting of  C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis C. dubliniensis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, A. fumigatus, A. nidulans, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor  and  Neosartorya fischeri.    
         [0052]    Under these circumstances, the amplification primers and oligonucleotide probes of the invention may be designed to a gene specific or genus specific region so as to be able to identify one or more, or most, or substantially all of the desired organisms of the target organism grouping. 
         [0053]    The test sample may comprise cells of the target yeast and/or fungal organism. The method may also comprise a step for releasing nucleic acid from any cells of the target yeast or fungal organism that may be present in said test sample. Ideally, the test sample is a lysate of an obtained sample from a patient (such as a swab, or blood, urine, saliva, a bronchial lavage, dental specimen, skin specimen, scalp specimen, transplant organ biopsy, stool, mucus, or discharge sample). The test samples may be a food sample, a water sample, an environmental sample, an end product, end product or in-process industrial sample. 
         [0054]    The invention also provides for the use of any one of SEQ ID NO.1 to SEQ ID NO.110 in a diagnostic assay for the presence of one or more yeast or fungal species. The species may be selected from the group consisting of  C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. dubliniensis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, A. fumigatus, N. fischeri, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor  and  A. nidulans.    
         [0055]    The invention also provides for kits for use in clinical diagnostics, theranostics, food safety diagnostics, industrial microbiology diagnostics, environmental monitoring, veterinary diagnostics, bio-terrorism diagnostics comprising one or more of the synthetic oligonucleotides of the invention. The kits may also comprise one or more articles selected from the group consisting of appropriate sample collecting instruments, reagent containers, buffers, labelling moieties, solutions, detergents and supplementary solutions. The invention also provides for use of the sequences, compositions, nucleotide fragments, assays, and kits of the invention in theranostics, Food safety diagnostics, Industrial microbiology diagnostics, Environmental monitoring, Veterinary diagnostics, Bio-terrorism diagnostics. 
         [0056]    The nucleic acid molecules, composition, kits or methods may be used in a diagnostic nucleic acid based assay for the detection of yeast and/or fungal species. 
         [0057]    The nucleic acid molecules, composition, kits or methods may be used in a diagnostic assay to measure yeast and/or fungal titres in a patient. The titres may be measured in vitro. 
         [0058]    The nucleic acid molecules, composition, kits or methods may be used in a method of assessing the efficacy of a treatment regime designed to reduce yeast and/or fungal titre in a patient comprising assessing the yeast and/or fungal titre in the patient (by in vivo methods or in vitro methods) at one or more key stages of the treatment regime. Suitable key stages may include before treatment, during treatment and after treatment. The treatment regime may comprise an antifungal agent, such as a pharmaceutical drug. The nucleic acid molecules, composition, kits or methods may be used in a diagnostic assay to measure potential yeast and/or fungal contamination, for example, in a hospital. The nucleic acid molecules, composition, kits or methods may be used in the identification and/or characterization of one or more disruptive agents that can be used to disrupt the P2, P2A or P2B gene function. Suitable disruptive agents may be selected from the group consisting of antisense RNA, PNA, siRNA. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0059]      FIG. 1 : Primers binding sites (grey highlights or bold text or bold and underlined) in P2B of  Candida albicans.    
           [0060]      FIG. 2 : Primers binding sites in P2 of  Aspergillus fumigatus . The amplified region of interest is underlined (Position 1 to 326). 
           [0061]      FIG. 3 : Binding site of  C. glabrata probe P 1-CglabP2B (bold and underlined) in the amplified fragment of P2B. PCR primers CglabP2B-F/CglabP2B-R are highlighted. 
           [0062]      FIG. 4 : Binding site of the  A. fumigatus  probe P1-AspP2 (bold and underlined) in the amplified fragment of P2B. PCR primers AspP2-F/AspP2-R are highlighted. 
           [0063]      FIG. 5 : Resulting amplification curve from Real-Time PCR assay based on the P2B gene for  C. glabrata  with P1-CglabP2B probe. Specificity of the assay was tested using a panel of DNA from 4 other  Candida  species and  Aspergillus fumigatus . The 3  C. glabrata  strains tested were detected and no cross-reaction was observed with DNA from other species. 
           [0064]      FIG. 6 . Resulting amplification curve from Real-time PCR based on the P2 gene for  A. fumigatus  with the P1-AspP2 probe. Specificity of the assay was tested against a panel of DNA from 6 closely related  Aspergillus  species and  C. albicans . The assay detected  A. fumigatus  but did not detect or cross-react with DNA from  C. albicans  or significantly with any of the  Aspergillus  species tested. 
           [0065]      FIG. 7 . Alignment of sequence information for  Aspergillus  species. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Materials and Methods 
     Cell Culture 
       [0066]      Candida  species were cultured in Sabouraud broth (4% wt/vol glucose, 1% wt/vol peptone, 1.5% agar) for 48 hours at 37° C. in a shaking incubator.  Aspergillus  species were cultured in Sabouraud broth (4% wt/vol glucose, 1% wt/vol peptone, 1.5% agar) or agar for 3-4 days at 25° C. 
       DNA Extraction 
       [0067]    Cells from  Candida  and  Aspergillus  spp. were pretreated with lyticase or zymolase enzymes prior to DNA isolation. DNA was isolated  Candida  and  Apergillus  spp. using the MagNA Pure System (Roche Molecular Systems) in combination with the MagNA pure Yeast and Bacterial isolation kit III. 
         [0000]    DNA sequencing of P2B/P2 gene regions in  Candida  and  Aspergillus  spp. 
         [0068]    The available sequences of the P2B genes of  Candida  and P2 genes of  Aspergillus  spp. were acquired from the NCBI database and aligned using Clustal W. Three annotated sequences for P2B of  C. albicans  (XM — 718047.1, XM — 717900.1 and AF317662.1) are available in the NCBI database and a sequence of high homology to P2B in  C. albicans  has been deposited for  Candida glabrata  (hypothetical protein, XM — 444905.1), which is considered here to be P2B. In addition to available sequences for P2 in  Aspergillus  spp., three presumptive P2 sequences are deposited as hypothetical proteins for  Aspergillus  spp. (XM — 001213622.1, XM — 001397764.1, XM — 658508.1). PCR Primers were designed (Table 1) and synthesized by an external company, TibMolBiol, Germany. Primer set CanP2B-F/CanP2B-R was designed to amplify a 335 by region of the P2B in  Candida  spp. from positions equivalent to by position 1 to position 335 in  C. albicans  (XM — 718047.1, XM — 717900.1) or position 8 to by position 342 of the P2B gene in  C. albicans  (AF317662.1). Primer set CanP2B-F/CanP2B-R may also in selected  Candida  species amplify a 326 by region of the P2A gene. Primer set CanP2B-F/CanP2B flR was designed to amplify a 352 by region of the P2B equivalent to by 8 to by 359 in  C. albicans  (AF317662.1). Primer set P2B-F/P2BinR1 were designed to amplify a 312 by region of the P2B gene from by position 1 to by position 312 in XM718047.1/XM 717900.1 and from by position 8 to by 319 of AF317662.1. Primer set CglabP2B-F/CglabP2B-R were designed to amplify the region in P2B equivalent to by position 24 to by position 158 in  C. glabrata  (XM — 444905.1). Primer set AspP2-F/AspP2-R were designed to amplify a region of P2 in  Aspergillus  spp. from base pair positions equivalent to 1 to position 326 in  A. fumigatus  (XM — 750250.1).  FIGS. 1 and 2  show the positions of the primer set CanP2B-F/CanP2B-R,CanP2B flR and P2BinR1 in  C. albicans  and AspP2-F/AspP2-R in  A. fumigatus  respectively. These primer sets were used to amplify regions of the P2B and P2 genes in other  Candida  and  Aspergillus  spp. respectively by conventional PCR on the iCycler BioRad PCR machine or the PTC200 Peltier thermocycler (MJ Research) using the reagents outlined in Table 2 and the thermocycling conditions described in Table 3 or modifications thereof. The PCR reaction products purified with Roche High Pure PCR Product Purification kit or with the ExoSAP-IT kit (USB) according to the manufacturers&#39; instructions were sent for sequencing to Sequiserve, Germany and were sequenced using the forward amplification primer CanP2B-F or AspP2-F. Sequence information was obtained for P2B gene regions for 8  Candida    spp. (   C. albicans, C. dubliniensis, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. guillermondii, C. lusitanie ) and P2 gene regions for 7  Aspergillus  species ( A. fumigatus, A. nidulans, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor ) and  Neosartorya fischeri.    
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 PCR primers designed for amplification of P2B 
               
               
                 gene region in  Candida  and P2 gene region in 
               
               
                   Aspergillus  spp. 
               
             
          
           
               
                   
                 Primer Name 
                 Primer Sequence 5′ to 3′ 
               
               
                   
                   
               
               
                   
                 CglabP2B-F 
                 ATTGTTGACCCAAGGTGGTAAC 
               
               
                   
                   
               
               
                   
                 CglabP2B-R 
                 TCGTCCAAGGACTTACCTTCCAA 
               
               
                   
                   
               
               
                   
                 CanP2B-F 
                 ATGAAATACTTAGCTGCTTAC 
               
               
                   
                   
               
               
                   
                 CanP2B-R 
                 TAATCGAATAAACCGAAACCCA 
               
               
                   
                   
               
               
                   
                 CanP2B-flR 
                 GCGACAAGCAATTTCTCT 
               
               
                   
                   
               
               
                   
                 CanP2BinR1 
                 ATCATCATCAGATTCTTCTTTG 
               
               
                   
                   
               
               
                   
                 AspP2-F 
                 ATGAAGCACCTCGCCGC 
               
               
                   
                   
               
               
                   
                 AspP2-R 
                 AGACCGAAGCCCATGTC 
               
               
                   
                   
               
               
                   
                 P2ForA 
                 GAGGAGCGCCT 
               
               
                   
                   
               
               
                   
                 P2ForB 
                 GAGGAGCGCCTC 
               
               
                   
                   
               
               
                   
                 P2ForC 
                 GGAGGAGCGCCTC 
               
               
                   
                   
               
               
                   
                 P2ForD 
                 TGAGGAGCGCCTC 
               
               
                   
                   
               
               
                   
                 P2RevA 
                 CCGGAGGGAACGGA 
               
               
                   
                   
               
               
                   
                 P2RevB 
                 CCGGAGGGAACGG 
               
               
                   
                   
               
               
                   
                 CAP2BF (For) 
                 ACCTCTCCATCAGCTTCTG 
               
               
                   
                   
               
               
                   
                 CAP2BR (Rev) 
                 TCAGCAATCAATTCTTGC 
               
               
                   
                   
               
               
                   
                 CGP2BF (For) 
                 ACCTCTGTCTTATCATCTGTCG 
               
               
                   
                   
               
               
                   
                 CKP2BR (Rev) 
                 CTCTTCGACGGACTTACC 
               
               
                   
                   
               
               
                   
                 CGP2BF (For) 
                 AAGAAGGTTATCGAATCTGTTG 
               
               
                   
                   
               
               
                   
                 CGP2BR (Rev) 
                 TTCGTCCAAGGACTTACC 
               
               
                   
                   
               
               
                   
                 CTP2BF (For) 
                 TCCGCTTTATTGGAACAAGTTG 
               
               
                   
                   
               
               
                   
                 CTP2BR (Rev) 
                 TTCTTGCAAGTCTTTACC 
               
               
                   
                   
               
               
                   
                 CPP2BF (For) 
                 TCCTCATTGTTGGAATCCGTTG 
               
               
                   
                   
               
               
                   
                 CPP2BR (Rev) 
                 CTCGTTGATGTCTTTACC 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 PCR reagents used to amplify P2B gene region in  Candida   
               
               
                 and P2 gene region in  Aspergillus  spp. 
               
             
          
           
               
                   
                   
                 SAMPLE  
               
               
                   
                 PCR Reaction Mix 
                 x1 
               
               
                   
                   
               
             
          
           
               
                   
                 10x Buffer (100 mM Tris HCl, 15 mM MgCl 2 ,  
                 5  
                 μl 
               
               
                   
                 500 mM KCl pH 8.3) 
                   
                   
               
               
                   
                 dNTP&#39;s Mix, Roche (10 mM dNTP) 
                 1  
                 μl 
               
               
                   
                 Primer Forward CanP2B-F or AspP2B-F (10 μM) 
                 1  
                 μl 
               
               
                   
                 Primer Reverse CanP2B-R CanP2B-flR,  
                 1  
                 μl 
               
               
                   
                 Can P2BinR or AspP2B-R (10 μM) 
                   
                   
               
               
                   
                 Polymerase TaqPol, Roche 1 U/μl 
                 1  
                 μl 
               
               
                   
                 H2O, Amgen/Accugene 
                 36-39  
                 μl 
               
               
                   
                 Genomic DNA Template 
                 2-5  
                 μl 
               
               
                   
                 TOTAL VOLUME 
                 50  
                 μl 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 PCR reaction conditions used to amplify P2B gene region in  
               
               
                   Candida  and P2 gene region in  Aspergillus  spp. 
               
               
                 PCR Thermal profile 
               
             
          
           
               
                   
                 Step 
                 Temp 
                 Time 
                   
               
               
                   
                   
               
               
                   
                 1 
                 94° C. 
                 1 min 
                 X 35 
               
               
                   
                 2 
                 50° C. 
                 1 min 
                   
               
               
                   
                 3 
                 72° C. 
                 1 min 
                   
               
               
                   
                 4 
                 72° C. 
                 7 min 
                   
               
               
                   
                 5 
                  8° C. 
                 Hold 
               
               
                   
                   
               
               
                   
                 Lid preheating was ON 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 TaqMan probes (5′-FAM and 3′-BHQ1 labels) 
               
               
                 based on the P2B/P2 gene for C. glabrata 
               
               
                 and  A .  fumigatus . 
               
             
          
           
               
                   
                 Probe Name 
                 Probe Sequence 5′to 3′ 
               
               
                   
                   
               
               
                   
                 P1-Cglab-P2B 
                 CAAGAAGGTTATCGAATCTGTTGGTATTG 
               
               
                   
                   
               
               
                   
                 P1-AspP2 
                 CCTGCCGCTGCCGGTGCCGCTGCC 
               
               
                   
                   
               
               
                   
                 P2FumP 
                 ACTACAGCTCGAAGATTA 
               
               
                   
                   
               
               
                   
                 P2FlavP 
                 ACGTTGAATGATTGAGAC 
               
               
                   
                   
               
               
                   
                 P2NigP 
                 TTGCGATTACAAGATGGAA 
               
               
                   
                   
               
               
                   
                 P2TerrP 
                 CTTCGGACTACTGCGATGA 
               
               
                   
                   
               
               
                   
                 CAP2BP 
                 ACCGCTTTATTGGAATCCGTTG 
               
               
                   
                   
               
               
                   
                 CKP2BP 
                 ATCCGACAAGTTAGACAAGTTAATC 
               
               
                   
                   
               
               
                   
                 CGP2BP 
                 AGAATCAACGAATTGTTGTCTGC 
               
               
                   
                   
               
               
                   
                 CTP2BP 
                 ATCTTCCAAATTAGACTTATTGTTGA 
               
               
                   
                   
               
               
                   
                 CPP2BP 
                 GAAGAATCAAGATTATCTACCTTGTTG 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Real-time PCR reagents 
               
             
          
           
               
                   
                 SAMPLE  
               
               
                 Preparation of PCR Reaction Mix 
                 x1 
               
               
                   
               
             
          
           
               
                 HybProb mix 10x conc. (LightCycler ® FastStartDNA  
                 2 
                 μl 
               
               
                 Master HybProbe Kit) 
                   
                   
               
               
                 MgCl 2  stock solution (Final conc. in reaction is 3 mM) 
                 1.6  
                 μl 
               
               
                 Probe P1-CglabP2B or P1-AspP2 
                 2 
                 μl 
               
               
                 Primer Forward CglabP2B-F or AspP2-F 
                 1 
                 μl 
               
               
                 Primer Reverse CglabP2B-R or AspP2-R 
                 1 
                 μl 
               
               
                 H 2 O PCR-grade 
                 10.4 
                 μl 
               
               
                 Genomic DNA Template 
                 2 
                 μl 
               
               
                 TOTAL VOLUME 
                 20 
                 μl 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Real-time PCR thermocycling conditions: 
               
               
                 PCR Thermal profile 
               
             
          
           
               
                 Cycle 
                 Step 
                 Temp 
                 Time 
                   
               
               
                   
               
             
          
           
               
                 Activation 
                 1 
                 95°  
                 C. 
                 10  
                 min 
                 X 50 
               
               
                 Amplification 
                 1 
                 95°  
                 C. 
                 10  
                 sec 
                   
               
               
                   
                 2 
                 62 or 65°  
                 C. 
                 20  
                 sec 
                   
               
               
                   
                 3 
                 70°  
                 C. 
                 10  
                 sec 
                   
               
             
          
           
               
                 Cooling 
                 1 
                 40°  
                 C. 
                 Hold 
                   
               
               
                   
               
               
                 The PCR was performed with LightCycler ® Roche PCR 
               
             
          
         
       
     
       Results 
     Primer and Probe Design 
       [0069]    The publicly available sequence information for the P2B gene in  Candida  spp. was aligned with the newly generated sequence information for the P2B gene in  Candida  spp. and analysed using bioinformatics tools. The sequence information available for the P2 gene in  Aspergillus  spp. was aligned with the newly generated sequence information for the P2 gene in  Aspergillus  spp. and analysed using bioinformatics tools. Species-specific probes were designed based on the compiled P2B and P2 sequence information for  Candida glabrata  and  Aspergillus fumigatus  respectively (Table 4).  FIGS. 3 and 4  show the relative positions of the PCR primers and TaqMan DNA probes for the amplification and detection of  C. glabrata  and  A. fumigatus  respectively. 
       Real-time PCR 
       [0070]    These probes were designed as TaqMan probes and demonstrated for species detection in real-time PCR on the LightCycler using reagents and thermocycling conditions detailed in Tables 5 and 6. For the  C. glabrata  real-time PCR assay based on the P2B gene, PCR primers CglabP2B-F/CglabP2B-R were combined with TaqMan probe P1-Cglab-P2B. The specificity of the assay for the detection of  C. glabrata  was confirmed by including DNA from a range of closely related  Candida  species and  A. fumigatus  in the  C. glabrata  real-time PCR assay. The assay detected  C. glabrata  but did not detect or cross-react with DNA from any other  Candida  species tested or with  A. fumigatus  DNA.  FIG. 5  shows the results of the  C. glabrata  real-time PCR assay and the specificity of the assay for  C. glabrata . For the  A. fumigatus  real-time PCR assay based on the P2 gene, PCR primers AspP2-F/AspP2-R were combined with TaqMan probe P1-AspP2. The specificity of the assay for the detection of  A. fumigatus  was confirmed by including DNA from a range of closely related  Aspergillus  species and  C. albicans  in the  A. fumigatus  real-time PCR assay. The assay detected  A. fumigatus  but did not detect or cross-react with DNA from  C. albicans  or any of the four  Aspergillus  species tested. Two additional  Aspergillus  spp. did show a small amount of cross reaction in the  A. fumigatus  real-time PCR assay but this can be eliminated with further assay optimisation.  FIG. 6  shows the results of the  A. fumigatus  real-time PCR assay. 
         [0071]    In so far as any sequence disclosed herein differs from its counterpart in the attached sequence listing in PatentIn3.3 software, the sequences within this body of text are to be considered as the correct version. 
         [0072]    The words “comprises/comprising” and the words “having/including” when used herein with reference to the present invention are used to specify the presence of stated features, integers, steps or components but does not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof. 
         [0073]    It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable sub-combination. 
       SEQ IDs 
       [0074]    Sites of probes, oligonucleotides etc. are shown in bold and underlined. N or x=any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t, h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific degeneracy options are indicated in parenthesis: e.g.: (a/g) is either A or G. 
         [0000]    
       
         
               
             
           
               
                 SEQ ID NO 1: 
               
               
                 CglabP2B-F 
               
               
                 ATTGTTGACCCAAGGTGGTAAC 
               
               
                   
               
               
                 SEQ ID NO 2: 
               
               
                 CglabP2B-R 
               
               
                 TCGTCCAAGGACTTACCTTCCAA 
               
               
                   
               
               
                 SEQ ID NO 3: 
               
               
                 CanP2B-F 
               
               
                 ATGAAATACTTAGCTGCTTAC 
               
               
                   
               
               
                 SEQ ID NO 4: 
               
               
                 CanP2B-R 
               
               
                 TAATCGAATAAACCGAAACCCA 
               
               
                   
               
               
                 SEQ ID NO 5: 
               
               
                 Can P2BflR1 
               
               
                 GCGACAAGCAATTTCTCT 
               
               
                   
               
               
                 SEQ ID NO 6: 
               
               
                 Can P2BinR1 
               
               
                 ATCATCATCAGATTCTTCTTTG 
               
               
                   
               
               
                 SEQ ID NO 7: 
               
               
                 AspP2-F 
               
               
                 ATGAAGCACCTCGCCGC 
               
               
                   
               
               
                 SEQ ID NO 8: 
               
               
                 AspP2-R 
               
               
                 AGACCGAAGCCCATGTC 
               
               
                   
               
               
                 SEQ ID NO 9: 
               
               
                 P1-Cglab-P2B 
               
               
                 CAAGAAGGTTATCGAATCTGTTGGTATTG 
               
               
                   
               
               
                 SEQ ID NO 10: 
               
               
                 P1-AspP2 
               
               
                 CCTGCCGCTGCCGGTGCCGCTGCC 
               
               
                   
               
               
                 SEQ ID NO 11: 
               
               
                 &gt;glab2897\(PF) sequences generated for  C . glabrata   
               
               
                 CTATTGTTGACCCAAGGTGGTAACGAATCTCCAGCTGCTGCTGACATC 
               
               
                   
               
               
                 AAGAAGGTTATCGAATCTGTTGGTATTGAAGCTGACGAAGCCAGAATC 
               
               
                   
               
               
                 AACGAATTGTTGTCTGCTTTGGAAGGTAAGTCCTTGGACGAATTGATC 
               
               
                   
               
               
                 GCTGAAGGTCAACAAAAGTTCGCCTCTGTTCCAGTTGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCCGCTGCTGCTGGTGGTGCCGCTGCCGGTGAAGCC 
               
               
                   
               
               
                 GCTGAAGAAAAGGAAGAAGAAGCTGCTGAAGAATCCGATGACGACATG 
               
               
                   
               
               
                 GGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 12: 
               
               
                 &gt;glab8018\(PF) sequences generated for  C . glabrata   
               
               
                 TTGTTGACCCAAGGTGGTAACGAATCTCCAGCTGCTGCTGACATCAAG 
               
               
                   
               
               
                 AAGGTTATCGAATCTGTTGGTATTGAAGCTGACGAAGCCAGAATCAAC 
               
               
                   
               
               
                 GAATTGTTGTCTGCTTTGGAAGGTAAGTCCTTGGACGAATTGATCGCT 
               
               
                   
               
               
                 GAAGGTCAACAAAAGTTCGCCTCTGTTCCAGTTGGTGGTGCTGCTGCT 
               
               
                   
               
               
                 GGTGGTGCTTCCGCTGCTGCTGGTGGTGCCGCTGCCGGTGAAGCCGCT 
               
               
                   
               
               
                 GAAGAAAAGGAAGAAGAAGCTGCTGAAGAATCCGATGACGACATGGGT 
               
               
                   
               
               
                 TTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 13: 
               
               
                 &gt;glab4692\(PF) sequences generated for  C . glabrata   
               
               
                 CTATTGTTGACCCAAGGTGGTAACGAATCTCCAGCTGCTGCTGACATC 
               
               
                   
               
               
                 AAGAAGGTTATCGAATCTGTTGGTATTGAAGCTGACGAAGCCAGAATC 
               
               
                   
               
               
                 AACGAATTGTTGTCTGCTTTGGAAGGTAAGTCCTTGGACGAATTGATC 
               
               
                   
               
               
                 GCTGAAGGTCAACAAAAGTTCGCCTCTGTTCCAGTTGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCCGCTGCTGCTGGTGGTGCCGCTGCCGGTGAAGCC 
               
               
                   
               
               
                 GCTGAAGAAAAGGAAGAAGAAGCTGCTGAAGAATCCGATGACGACATG 
               
               
                   
               
               
                 GGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 14: 
               
               
                 &gt;glabl38\(PF) sequences generated for  C . glabrata   
               
               
                 CTATTGTTGACCCAAGGTGGTAACGAATCTCCAGCTGCTGCTGACATC 
               
               
                   
               
               
                 AAGAAGGTTATCGAATCTGTTGGTATTGAAGCTGACGAAGCTAGAATC 
               
               
                   
               
               
                 AACGAATTGTTGTCTGCTTTGGAAGGTAAGTCCTTGGACGAATTGATC 
               
               
                   
               
               
                 GCTGAAGGTCAACAAAAGTTCGCCTCTGTTCCAGTTGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCCGCTGCTGCTGGTGGTGCCGCTGCCGGTGAAGCT 
               
               
                   
               
               
                 GCTGAAGAAAAGGAAGAAGAAGCTGCTGAAGAATCCGATGACGACATG 
               
               
                   
               
               
                 GGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 15: 
               
               
                 &gt;krusei3165\(P2BF) sequence generated for  C .  krusei   
               
               
                 TTACTCTTAGTCAATGCTGGTAAAACCGCACCATCTGCTGCAGATGTT 
               
               
                   
               
               
                 ACCTCTGTCTTATCATCTGTCGGTATCGAAGTTGAATCCGACAAGTTA 
               
               
                   
               
               
                 GACAAGTTAATCTCCGAATTAGAAGGTAAGTCCGTCGAAGAGTTGATT 
               
               
                   
               
               
                 GCTGAAGGTACTGAAAAGATGGCTTCTGCTCCAGGTGCAGCAGCTGCT 
               
               
                   
               
               
                 CCAGCTTCTGGTGCAGGTGCTTCCACCGAATCTGCTGCAGCAGAAGAA 
               
               
                   
               
               
                 GTTGAAGAAGAAAAGGAAGAATCCGATGATGACATGGGTTTCGGTTTA 
               
               
                   
               
               
                 TTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 16: 
               
               
                 &gt;krusei9560\(P2BF) sequence generated for  C .  krusei   
               
               
                 CATCTGCTGCAGATGTTACCTCTGTCTTATCATCTGTCGGTATCGAAG 
               
               
                   
               
               
                 TTGAATCCGACAAGTTAGACAAGTTAATCTCCGAATTAGAAGGTAAGT 
               
               
                   
               
               
                 CCGTCGAAGAGTTGATTGCTGAAGGTACTGAAAAGATGGCTTCTGCTC 
               
               
                   
               
               
                 CAGGTGCAGCAGCTGCTCCAGCTTCTGGTGCAGGTGCTTCCACCGAAT 
               
               
                   
               
               
                 CTGCTGCAGCAGAAGAAGTTGAAGAAGAAAAGGAAGAATCCGATGATG 
               
               
                   
               
               
                 ACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 17: 
               
               
                 &gt;krusei60554P2BF) sequence generated for  C .  krusei   
               
               
                 CATCTGCTGCAGATGTTACCTCTGTCTTATCATCTGTCGGTATCGAAG 
               
               
                   
               
               
                 TTGAATCCGACAAGTTAGACAAGTTAATCTCCGAATTAGAAGGTAAGT 
               
               
                   
               
               
                 CCGTCGAAGAGTTGATTGCTGAAGGTACTGAAAAGATGGCTTCTGCTC 
               
               
                   
               
               
                 CAGGTGCAGCAGCTGCTCCAGCTTCTGGTGCAGGTGCTTCCACCGAAT 
               
               
                   
               
               
                 CTGCTGCAGCAGAAGAAGTTGAAGAAGAAAAGGAAGAATCCGATGATG 
               
               
                   
               
               
                 ACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 18: 
               
               
                 &gt;krusei573\(P2B) sequence generated for  C .  krusei   
               
               
                 TTACTCTTAGTACATGCTGGTAAAACCGCACCATCTGCTGCAGATGTT 
               
               
                   
               
               
                 ACCTCTGTCTTATCATCTGTCGGTATCGAAGTTGAATCCGACAAGTTA 
               
               
                   
               
               
                 GACAAGTTAATCTCCGAATTAGAAGGTAAGTCCGTCGAAGAGTTGATT 
               
               
                   
               
               
                 GCTGAAGGTACTGAAAAGATGGCTTCTGCTCCAGGTGCAGCAGCTGCT 
               
               
                   
               
               
                 CCAGCTTCTGGTGCAGGTGCTTCCACCGAATCTGCTGCAGCAGAAGAA 
               
               
                   
               
               
                 GTTGAAGAAGAAAAGGAAGAATCCGATGATGACATGGGTTTCGGTTTA 
               
               
                   
               
               
                 TTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 19: 
               
               
                 &gt;CA562P2B sequence generated for  C . albicans   
               
               
                 TTATTGTTAGTTCAAGGTGGTAACACCTCTCCATCAGCTTCTGATATC 
               
               
                   
               
               
                 ACCGCTTTATTGGAATCCGTTGGTGTTGAAGCCGAAGAATCCAGATTA 
               
               
                   
               
               
                 CAAGCTTTATTGAAAGATTTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTAGCTTCTGTCCCATCCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCTGCCTCTGCTGGTGCCGCTGCTGGTGGTGCTGCT 
               
               
                   
               
               
                 GAAGCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGAT 
               
               
                   
               
               
                 GATATGGGTTTCGGTTTATTCGATTAGAGAAATTGCTTGTCGC 
               
               
                   
               
               
                 SEQ ID NO 20: 
               
               
                 &gt;A765\(P2BF) P2B sequence generated for  C . albicans   
               
               
                 TTATTGTTAGTTCAAGGTGGTAACACCTCTCCATCAGCTTCTGATATC 
               
               
                   
               
               
                 ACCGCTTTATTGGAATCCGTTGGTGTTGAAGCCGAAGAATCCAGATTA 
               
               
                   
               
               
                 CAAGCTTTATTGAAAGATTTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTAGCTTCTGTCCCATCCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCTGCCTCTGCTGGTGCCGCTGCTGGTGGTGCTGCT 
               
               
                   
               
               
                 GAAGCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGAT 
               
               
                   
               
               
                 GATATGGGTTTCGGTTTATTCGATTAGAGAAATTGCTTGTCGC 
               
               
                   
               
               
                 SEQ ID NO 21: 
               
               
                 &gt;A3156\(P2BF) P2B sequence generated for  C . albicans   
               
               
                 TTATTGTTAGTTCAAGGTGGTAACACCTCTCCATCAGCTTCTGATATC 
               
               
                   
               
               
                 ACCGCTTTATTGGAATCCGTTGGTGTTGAAGCCGAAGAATCCAGATTA 
               
               
                   
               
               
                 CAAGCTTTATTGAAAGATTTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTAGCTTCTGTCCCATCCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCTGCCTCTGCTGGTGCCGCTGCTGGTGGTGCTGCT 
               
               
                   
               
               
                 GAAGCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGAT 
               
               
                   
               
               
                 GATATGGGTTTCGGTTTATTCGATTAGAGAAATTGCTTGTCGC 
               
               
                   
               
               
                 SEQ ID NO 22: 
               
               
                 &gt;A2700\(P2BF) P2B sequence generated for  C . albicans   
               
               
                 TTATTGTTAGTTCAAGGTGGTAACACCTCTCCATCAGCTTCTGATATC 
               
               
                   
               
               
                 ACCGCTTTATTGGAATCCGTTGGTGTTGAAGCYGAAGAATCCAGATTA 
               
               
                   
               
               
                 CAAGCTTTATTGAAAGATTTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTAGCTTCTGTCCCATCCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCTGCCTCTRCTGGTGCCGCTGCTGGYGGTGCTGCY 
               
               
                   
               
               
                 GAAGCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGAT 
               
               
                   
               
               
                 GATATGGGTTTCGGTTTATTCGATTAGAGAAATTGCTTGTCGC 
               
               
                   
               
               
                 SEQ ID NO 23: 
               
               
                 &gt;D3949\(P2BF) P2B sequence generated for  
               
               
                   C . dubliniensis   
               
               
                 TTGTTGTTAGTTCAAGGTGGTAACGCCACTCCATCAGCTTCTGATATC 
               
               
                   
               
               
                 AGCGCTGTCTTGGAAACTGTTGGTGTTGAAGCCGAAGAATCCAGATTA 
               
               
                   
               
               
                 CAAGCTTTATTGAAAGATTTGGAAGGTAAAGATTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTAGCTTCTGTCCCAACCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCCGGTTCTGCTGGTGCCGCTTCTGGTGCCGCTGCT 
               
               
                   
               
               
                 GAAGCTGAAGAAGAAAAAGAAGAAGAAGCTAAAGAAGAATCTGATGAT 
               
               
                   
               
               
                 GATATGGGTTTCGGTTTATTCGATTAGAGAAATTGCTTGTCGC 
               
               
                   
               
               
                 SEQ ID NO 24: 
               
               
                 &gt;D7987\(P2BF) sequence generated for  
               
               
                   C . dubliniensis   
               
               
                 TTGTTGTTAGTTCAAGGTGGTAACGCCACTCCATCAGCTTCTGATATC 
               
               
                   
               
               
                 AGCGCTGTCTTGGAAACTGTTGGTGTTGAAGCCGAAGAATCCAGATTA 
               
               
                   
               
               
                 CAAGCTTTATTGAAAGATTTGGAAGGTAAAGATTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTAGCTTCTGTCCCAACCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGGTGGTGCTTCCGGTTCTGCTGGTGCCGCTTCTGGTGCCGCTGCT 
               
               
                   
               
               
                 GAAGCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGAT 
               
               
                   
               
               
                 GATA 
               
               
                   
               
               
                 SEQ ID NO 25: 
               
               
                 &gt;CP604\(CanP2B-FOR&gt;&gt;&lt;&lt;REV) P2B sequences 
               
               
                 generated for  C . parapsilosis   
               
               
                 ATGAAATACTTAGCTGCTTACTTATTATTGGTCCAAGGTGGTAACGCC 
               
               
                   
               
               
                 TCCCCATCTGCTTCAGACATCTCCTCATTGTTGGAATCCGTTGGTGTT 
               
               
                   
               
               
                 GAAGTTGAAGAATCAAGATTATCTACCTTGTTGAAAGACTTGGAAGGT 
               
               
                   
               
               
                 AAAGACATCAACGAGTTGATTGCTGAAGGTAACACCAAATTGGCCTCA 
               
               
                   
               
               
                 GTTCCATCTGGTGGTGCTGCTGTTGCTTCCGGTTCTGGTGCTTCTGGT 
               
               
                   
               
               
                 GCCGCTGCTGGTGGTGCTGCTGAAGAAGCTAAGGAAGAAGCCAAGGAA 
               
               
                   
               
               
                 GAAGAAAAGGAAGAATCTGATGATGACATGGGTTTCGGTTTATTCGAT 
               
               
                   
               
               
                 TA 
               
               
                   
               
               
                 SEQ ID NO 26: 
               
               
                 &gt;P2194\(P2BF) sequences generated for  
               
               
                   C . parapsilosis   
               
               
                 TTATTATTGGTCCAAGGTGGTAACGCCTCCCCATCTGCTTCAGACATC 
               
               
                   
               
               
                 TCCTCATTGTTGGAATCCGTTGGTGTTGAAGTTGAAGAATCAAGATTA 
               
               
                   
               
               
                 TCTACCTTGTTGAAAGACTTGGAAGGTAAAGACATCAACGAGTTGATT 
               
               
                   
               
               
                 GCTGAAGGTAACACCAAATTGGCCTCAGTTCCATCTGGTGGTGCTGCT 
               
               
                   
               
               
                 GTTGCTTCCGGTTCTGGTGCTTCTGGTGCCGCTGCTGGTGGTGCTGCT 
               
               
                   
               
               
                 GAAGAAGCTAAGGAAGAAGCCAAGGAAGAAGAAAAGGAAGAATCTGAT 
               
               
                   
               
               
                 GATGACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 27: 
               
               
                 &gt;P96141\(P2BF) sequences generated for 
               
               
                   C . parapsilosis   
               
               
                 GGTGGTAACGCCTCCCCATCCGCTTCAGACATCTCATCCTTGTTGGAA 
               
               
                   
               
               
                 TCCGTTGGTGTTGAAGTTGAAGAATCAAGATTGTCCCTCTTGTTGAAA 
               
               
                   
               
               
                 GACTTGGAAGGTAAAGACATCAACGAATTGATTGCTGAAGGTAACACC 
               
               
                   
               
               
                 AAGTTGGCTTCAGTTCCAACTGGTGGTGCTGCTGTTGCTTCTGGTTCT 
               
               
                   
               
               
                 GGTGCTTCAGGTGCCGCTGCTGGTGGTGCTGCTGAAGAAGCCAAAGAA 
               
               
                   
               
               
                 GAATCTGATGATGAT 
               
               
                   
               
               
                 SEQ ID NO 28: 
               
               
                 &gt;CT94\(P2BF) sequences generated for  C . tropicalis   
               
               
                 GGTGGTAACGCTTCCCCATCTGCTTCTGACATCTCCGCTTTATTGGAA 
               
               
                   
               
               
                 CAAGTTGGTGCTGAAGTTGAATCTTCCAAATTAGACTTATTGTTGAAA 
               
               
                   
               
               
                 GAATTGGAAGGTAAAGACTTGCAAGAATTGATTGCCGAAGGTAACACT 
               
               
                   
               
               
                 AAATTCGCCTCTGTCCCATCCGGTGGTGCTGCTGCTGCTTCYTCTGGT 
               
               
                   
               
               
                 TCCGCTGCCGCTGCTGGTGGTGCCGCTGCCGAAGCTGAAGAAGAAAAA 
               
               
                   
               
               
                 GAAGAAGAAGCCAAAGAAGAATCTGATGATGAT 
               
               
                   
               
               
                 SEQ ID NO 29: 
               
               
                 &gt;CT2311\(P2BF) sequences generated for  
               
               
                   C . tropicalis   
               
               
                 GGTGGTAACGCTTCCCCATCTGCTTCTGACATCTCCGCTTTATTGGAA 
               
               
                   
               
               
                 CAAGTTGGTGCTGAAGTTGAATCTTCCAAATTAGACTTATTGTTGAAA 
               
               
                   
               
               
                 GAATTGGAAGGTAAAGACTTGCAAGAATTGATTGCCGAAGGTAACACT 
               
               
                   
               
               
                 AAATTCGCCTCTGTCCCATCCGGTGGTGCTGCTGCTGCTTCCTCTGGT 
               
               
                   
               
               
                 TCCGCTGCCGCTGCTGGTGGTGCCGCTGCCGAAGCTGAAGAAGAAAAA 
               
               
                   
               
               
                 GAAGAAGAAGCCAAAGAAGAATCTGATGATGAT 
               
               
                   
               
               
                 SEQ ID NO 30: 
               
               
                 &gt;T-8157\(PF) sequences generated for  C . tropicalis   
               
               
                 TTATTATTAGTCCAAGGTGGTAACRCTTCCCCATCTGCTTCTGACATC 
               
               
                   
               
               
                 TCCGCTTTATTGGAACAAGTTGGTGCTGAAGTTGAATCTTCCAAATTA 
               
               
                   
               
               
                 GACTTATTGTTGAAAGAATTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCCGAAGGTAACACTAAATTCGCCTCTGTCCCATCCGGTGGTGCTGCY 
               
               
                   
               
               
                 GCTGCTTCCTCTGGTTCCGCTGCCGCTGCTGGTGGTGCCGCTGCCGAA 
               
               
                   
               
               
                 GCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGATGAT 
               
               
                   
               
               
                 SEQ ID NO 31: 
               
               
                 &gt;T-2424\(PF) sequences generated for  C . tropicalis   
               
               
                 TTATTATTAGTCCAAGGTGGTAACGCTTCCCCATCTGCTTCTGACATC 
               
               
                   
               
               
                 TCCGCTTTATTGGAACAAGTTGGTGCTGAAGTTGAATCTTCCAAATTA 
               
               
                   
               
               
                 GACTTATTGTTGAAAGAATTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCCGAAGGTAACACTAAATTCGCCTCTGTCCCATCCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGCTTCCTCTGGTTCCGCTGCCGCTGCTGGTGGTGCCGCTGCCGAA 
               
               
                   
               
               
                 GCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGATGAT 
               
               
                   
               
               
                 SEQ ID NO 32: 
               
               
                 &gt;T-3895\(PF) sequences generated for  C . tropicalis   
               
               
                 TTATTATTAGTCCAAGGTGGTAACGCTTCCCCATCTGCTTCTGACATC 
               
               
                   
               
               
                 TCCGCTTTATTGGAACAAGTTGGTGCTGAAGTTGAATCTTCCAAATTA 
               
               
                   
               
               
                 GACTTATTGTTGAAAGAATTGGAAGGTAAAGACTTGCAAGAATTGATT 
               
               
                   
               
               
                 GCCGAAGGTAACACTAAATTCGCCTCTGTCCCATCCGGTGGTGCTGCT 
               
               
                   
               
               
                 GCTGCTTCTTCTGGTTCCGCTGCCGCTGCTGGTGGTGCCGCTGCCGAA 
               
               
                   
               
               
                 GCTGAAGAAGAAAAAGAAGAAGAAGCCAAAGAAGAATCTGATGATGAT 
               
               
                   
               
               
                 SEQ ID NO 33: 
               
               
                 &gt;guill2672\(P2BF) sequences generated for 
               
               
                   C . guilliermondii   
               
               
                 TTRTTGTTGGTKRACGCCGGTAACACCTCCCCATCTGCTGCTGACATC 
               
               
                   
               
               
                 AAGGCTGTCTTGGAGTCGGTTTCCATAGAAGTTGACGACGAGAAGGTG 
               
               
                   
               
               
                 TCCAAGTTGTTGAGCGAAGTTGAGGGAAAGAATGCTGAAGAATTGATC 
               
               
                   
               
               
                 GCTGAAGGTAACGAAAAATTGTCTTCTGTTCCAACTGGTGGACCAGCT 
               
               
                   
               
               
                 GCTGCTTCCTCTGGATCTGCTGCCGCTGCCGATGCTCCTGCTGCCGAA 
               
               
                   
               
               
                 GAGGCCGCTGAGGAGGCCGCTGAGGAGTCTGACGACGACATGGGTTTC 
               
               
                   
               
               
                 GGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 34: 
               
               
                 &gt;guill6021\(P2BF) sequences generated for 
               
               
                   C . guilliermondii   
               
               
                 TTRTTGTTGGTKRACGCCGGTAACACCTCCCCATCTGCTGCTGACATC 
               
               
                   
               
               
                 AAGGCTGTCTTGGAGTCGGTTTCCATAGAAGTTGACGACGAGAAGGTG 
               
               
                   
               
               
                 TCCAAGTTGTTGAGCGAAGTTGAGGGAAAGAATGCTGAAGAATTGATC 
               
               
                   
               
               
                 GCTGAAGGTAACGAAAAATTGTCTTCTGTTCCAACTGGTGGACCAGCT 
               
               
                   
               
               
                 GCTGCTTCCTCTGGATCTGCTGCCGCTGCCGATGCTCCTGCTGCCGAA 
               
               
                   
               
               
                 GAGGCCGCTGAGGAGGCCGCTGAGGAGTCTGACGACGACATGGGTTTC 
               
               
                   
               
               
                 GGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 35: 
               
               
                 &gt;lus7270\(P2BF) sequences generated for  
               
               
                   C . lusitanie   
               
               
                 TTATTGTTGGTCAACGCTGGTAACACCGCCCCATCTGCTGCTGACGTC 
               
               
                   
               
               
                 AAGAAGGTCTTGGAATCCGTCTCTATTGAGGTTGAGGACGACAAGGTT 
               
               
                   
               
               
                 GAGAAGTTGTTGGCTGAAGTTGAAGGCAAGAACGTCGAAGAGTTGATT 
               
               
                   
               
               
                 GCCGAGGGTAACGAGAAGTTGTCTTCTGTTCCATCTGGTGCTCCAGCT 
               
               
                   
               
               
                 GCTGCTGGTGCCGCTGCTGCTTCTGGTTCTACTGAGGCTGCTGCTGAA 
               
               
                   
               
               
                 GAGCCACAAGAAGAAGAGAAGGAGGAGTCTGACGACGACATGGGTTTC 
               
               
                   
               
               
                 GGTTTATTCGATTA 
               
               
                 SEQ ID NO 36: 
               
               
                 &gt;AF419.64\(AspP2-F) P2 sequences generated for 
               
               
                   A . fumigatus   
               
               
                 TTACCTCCTCCTCGCCCTTGCTGGCAACACCTCCCCGTCCTCTGAGGA 
               
               
                   
               
               
                 TGTCAAGGCCGTCCTCTCTTCCGTTGGCATTGATGCCGATGAGGAGCG 
               
               
                   
               
               
                 CCTGAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 TAGTAACTACAGCTCGAAGATTACAGACTGGGAATTTTGGACTGGCGC 
               
               
                   
               
               
                 TGACATCGAACTCTACAACAGCTCATTGCCGAGGGTTCCACCAAGCTC 
               
               
                   
               
               
                 GCTTCCGTTCCCTCCGGTGGTGCTGCCGCCGCTGCTCCTGCCGCTGCC 
               
               
                   
               
               
                 GGTGCCGCTGCCGGTGGTGCTGCTGCTCCTGCCGCTGAGGAGAAGAAG 
               
               
                   
               
               
                 GAGGAGGAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 37: 
               
               
                 &gt;F-6951\(APF) P2 sequences generated for 
               
               
                   A . fumigatus   
               
               
                 CCTCCTCCTCGCCCTTGCTGGCAACACCTCCCCGTCCTCTGAGGATGT 
               
               
                   
               
               
                 CAAGGCCGTCCTCTCTTCCGTTGGCATTGATGCCGATGAGGAGCGCCT 
               
               
                   
               
               
                 GAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTCCAGGAGGTTAG 
               
               
                   
               
               
                 TAACTACAGCTCGAAGATTACAGACTGGGAATTTTGGACTGGCGCTGA 
               
               
                   
               
               
                 CATCGAACTCTACAACAGCTCATCGCCGAGGGTTCCACCAAGCTCGCT 
               
               
                   
               
               
                 TCCGTTCCCTCCGGTGGTGCTGCCGCCGCTGCTCCTGCCGCTGCCGGT 
               
               
                   
               
               
                 GCCGCTGCCGGTGGTGCTGCTGCTCCTGCCGCTGAGGAGAAGAAGGAG 
               
               
                   
               
               
                 GAGGAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 38: 
               
               
                 &gt;F-133-61\(APF) P2 sequences generated for 
               
               
                   A . fumigatus   
               
               
                 GCAACACCTCCCCGTCCTCTGAGGATGTCAAGGCCGTCCTCTCTTCCG 
               
               
                   
               
               
                 TTGGCATTGATGCCGATGAGGAGCGCCTGAACAAGCTCATTGCTGAGC 
               
               
                   
               
               
                 TCGAGGGCAAGGACCTCCAGGAGGTTAGTAACTACAGCTCGAAGATTA 
               
               
                   
               
               
                 CAGACTGGGAATTTTGGACTGGCGCTGACATCGAACTCTACAACAGCT 
               
               
                   
               
               
                 CATTGCCGAGGGTTCCACCAAGCTCGCTTCCGTTCCCTCCGGTGGTGC 
               
               
                   
               
               
                 TGCCGCCGCTGCTCCTGCCGCTGCCGGTGCCGCTGCCGGTGGTGCTGC 
               
               
                   
               
               
                 TGCTCCTGCCGCTGAGGAGAAGAAGGAGGAGGAGAAGGAGGAGTCCGA 
               
               
                   
               
               
                 CGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 39: 
               
               
                 &gt;AF493.61\(Asp2F) P2 sequences generated for 
               
               
                   A . fumigatus   
               
               
                 TTACCTCCTCCTCGCCCTTGCTGGCAACACCTCCCCGTCCTCTGAGGA 
               
               
                   
               
               
                 TGTCAAGGCCGTCCTCTCTTCCGTTGGCATTGATGCCGATGAGGAGCG 
               
               
                   
               
               
                 CCTGAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 TAGTAACTACAGCTCGAAGATTACAGACTGGGAATTTTGGACTGGCGC 
               
               
                   
               
               
                 TGACATCGAACTCTACAACAGCTCATTGCCGAGGGTTCCACCAAGCTC 
               
               
                   
               
               
                 GCTTCCGTTCCCTCCGGTGGTGCTGCCGCCGCTGCTCCTGCCGCTGCC 
               
               
                   
               
               
                 GGTGCCGCTGCCGGTGGTGCTGCTGCTCCTGCCGCTGAGGAGAAGAAG 
               
               
                   
               
               
                 GAGGAGGAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 40: 
               
               
                 &gt;AF1085-P2\(AspP2-F) P2 sequences generated for 
               
               
                   N . fischeri   
               
               
                 TTACCTCCTCCTCGCCCTTGCTGGCAACACCTCCCCCTCCGCTGAGGA 
               
               
                   
               
               
                 TGTCAAGGCCGTCCTCTCTTCCGTCGGCATTGACGCCGATGAGGAGCG 
               
               
                   
               
               
                 CCTGAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 TAGTACACACGGCTTGAATATTACCGACTGAGAATTTTGGACCGGCGC 
               
               
                   
               
               
                 TGACATCGATTTCTACAACAGCTGATCGCTGAGGGTTCCGCCAAGCTC 
               
               
                   
               
               
                 GCTTCCGTTCCCTCCGGTGGTGCCGGTGGTGCCGCTGCTCCTGCCGCT 
               
               
                   
               
               
                 GGCGGTGCCGCTGCCGGTGGTGCTGCTGCCGCTCCCGCCGAAGAGAAG 
               
               
                   
               
               
                 GAGGAGGAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 41: 
               
               
                 &gt;Nid100-2\(P2F) P2B sequence for  A .  nidulans   
               
               
                 CTACCTCCTCCTCGCCCTTGCTGGCAACGAGTCTCCCTCCGCCTCCGA 
               
               
                   
               
               
                 CATCAAGGAGGTTCTCTCTTCCGTCGGTGTTGACGCCGACGACGAGCG 
               
               
                   
               
               
                 CCTCGAGAAGCTCATTGCTGAGCTCCAGGGCAAGGACATCAACGAGGT 
               
               
                   
               
               
                 TCGTTATTGCATATAGAGTTGGAAGACGCGGACTGCGGGCTAACGATA 
               
               
                   
               
               
                 ATCTTTAAACAGCTGATCGCTGAGGGTACCACCAAGCTTGCCTCCGTT 
               
               
                   
               
               
                 CCCTCCGGCGGTGCTGGTGGTGCTGCCCCTGCTGCCGCTGCCGGTGGT 
               
               
                   
               
               
                 GCTGCTGCTGCCGAGGCCCCCGCTGCTGAGAAGGAGGAGGAGAAGGAG 
               
               
                   
               
               
                 GAGTCCGATGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 42: 
               
               
                 &gt;Nid7063\(P2F) P2B sequence for  A .  nidulans   
               
               
                 CCTTGCTGGCAACGAGTCTCCCTCCGCCTCCGACATCAAGGAGGTTCT 
               
               
                   
               
               
                 CTCTTCCGTCGGTGTTGACGCCGACGACGAGCGCCTCGAGAAGCTCAT 
               
               
                   
               
               
                 TGCTGAGCTCCAGGGCAAGGACATCAACGAGGTTCGTTATTGCATAGG 
               
               
                   
               
               
                 GTTGGAAGACGCGGACAGCGGGCTAACGATAATCTTCTGAACAGCTGA 
               
               
                   
               
               
                 TCGCTGAGGGTACCACCAAGCTTGCCTCCGTTCCCACCGGCGGTGCTG 
               
               
                   
               
               
                 GTGCTGCTGCCCCTGCTGCCGCTGCCGGTGGTGCTGCCGCTGCCGAGG 
               
               
                   
               
               
                 CTCCCGCTGCTGAGAAGGAGGAGGAGAAGGAGGAGTCCGATGAGGACA 
               
               
                   
               
               
                 TGGGCTTCGGTCT 
               
               
                 SEQ ID NO 43: 
               
               
                 &gt;AC5138\(AspP2-F) P2 sequences generated for  
               
               
                   A . clavatus   
               
               
                 TTACCTCCTCCTCGCCCTTGGTGGCAACGCCAGCCCCTCCGCTGCTGA 
               
               
                   
               
               
                 TGTTAAGGAGGTTCTCTCTTCTGTCGGCATTGATGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTTCAGGAGGT 
               
               
                   
               
               
                 TAGTTTTGCGCTGGTCTACGAGAGGAAGATTGTGACAAGATGCTAACG 
               
               
                   
               
               
                 GAAAATTTCTTCAACAGCTGATTGCTGAGGGTTCCACCAAGCTCGCTT 
               
               
                   
               
               
                 CCATTCCCTCCGGCGGTGCTGGTGGTGCTGCCCCCGCCGCTGGCGGTG 
               
               
                   
               
               
                 CTGCCGCCGGTGGTGCTGCTGAGGCCGCTCCCGCTGAGGAGAAGGAGG 
               
               
                   
               
               
                 AGGAGAAGGAGGAGTCCGACGACGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 44: 
               
               
                 &gt;AN1329399-P2\(AspP2-F) P2 sequences generated  
               
               
                 for  A . niger   
               
               
                 CTACCTTCTGTTGGCCCTTGCTGGCAACAACACCCCCTCCGCTGAGGA 
               
               
                   
               
               
                 CATCAAGTCCGTCCTCTCCGCCGTCGGCATTGACGCTGAGGAGGAGCG 
               
               
                   
               
               
                 CCTCCAGAAGCTCCTTGCTGAGCTTGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 CAGTTAACGCCCTTAAAATCTACCAAGAAATTTTGCGATTACAAGATG 
               
               
                   
               
               
                 GAATACTGACAATGGGTTTTCTACAACAGCTCATCTCCGAGGGTACCC 
               
               
                   
               
               
                 AGAAGCTCGCTTCCGTTCCCTCCGGTGGTGCCGGTGCTGCTGCCGCTG 
               
               
                   
               
               
                 CCCCCGCTGCCGGTGGCGCCGCTGCTGCTGAGGCTCCCGCTGAGGAGA 
               
               
                   
               
               
                 AGAAGGAGGAGGCTGCTGAGGAGTCCGATGAGGACATGGGCTTCGGTC 
               
               
                   
               
               
                 T 
               
               
                   
               
               
                 SEQ ID NO 45: 
               
               
                 &gt;AN2864\(Asp2F) sequences generated for  A . niger   
               
               
                 CTACCTTCTGTTGGCCCTTGCTGGCAACAACACCCCCTCCGCTGAGGA 
               
               
                   
               
               
                 CATCAAGTCCGTCCTCTCCGCCGTCGGCATTGACGCTGAGGAGGAGCG 
               
               
                   
               
               
                 CCTCCAGAAGCTCCTTGCTGAGCTTGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 CAGTTAACGCCCTTAAAATCTACCAAGAAATTTTGCGATTACAAGATG 
               
               
                   
               
               
                 GAATACTGACAATGGTTTTTCTACAACAGCTCATCTCCGAGGGTACCC 
               
               
                   
               
               
                 AGAAGCTCGCTTCCGTTCCCTCCGGTGGTGCCGGTGCTGCTGCCGCTG 
               
               
                   
               
               
                 CCCCCGCTGCCGGTGGCGCCGCTGCTGCTGAGGCTCCCGCTGAGGAGA 
               
               
                   
               
               
                 AGAAGGAGGAGGCTGCTGAGGAGTCCGATGAGGACATGGGCTTCGGTC 
               
               
                   
               
               
                 T 
               
               
                   
               
               
                 SEQ ID NO 46: 
               
               
                 &gt;AT118.46\(AspP2 -F) P2 sequences generated for 
               
               
                 
                   A.terreus 
                 
               
               
                 TTCCTTCTCCTCGGCCTTGCCGGCAACACCTCCCCCTCTGCTGAGGAC 
               
               
                   
               
               
                 ATCAAGGCTGTCCTCTCCTCCGTCGGCATTGACGCTGATGAGGAGCGC 
               
               
                   
               
               
                 CTCGGCCAGCTCCTGAAGGAGCTCGAGGGCAAGGACATCCAGGAGGTT 
               
               
                   
               
               
                 AGTGATCACCATAACTTCGGACTACTGCGATGAGAACGCGCCATACTA 
               
               
                   
               
               
                 ACGGATTATACAGCTCATCGCTCAGGGCTCTGAGAAGCTCGCTTCCGT 
               
               
                   
               
               
                 TCCCTCTGGCGGTGCTGCCGCTGCTGCTGCTCCCGCCGCTGCCGCTGG 
               
               
                   
               
               
                 CGGTGACGCTGCTGCCCCCGCTGAGAAGAAGGAGGAGGAGAAGGAGGA 
               
               
                   
               
               
                 GGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 47: 
               
               
                 &gt;AF2008\(AspP2-F) P2 sequences generated for 
               
               
                   A . flavus   
               
               
                 TTACCTCCTCCTCGCCCTCGCTGGCAACTCCACCCCCTCCGTTGAGGA 
               
               
                   
               
               
                 CATCAAGAGCGTTCTCTCTTCCGTCGGTATTGATGCCGATGAGGAGCG 
               
               
                   
               
               
                 CCTCCAGAAGGTCATCTCCGAGCTCGAGGGCAAGGACCTCCAGCAGGT 
               
               
                   
               
               
                 TCGTAATACGTTGAATGATTGAGACATAGGGCGCCTGCTGACCATCTA 
               
               
                   
               
               
                 CTCACTACAGCTGATCACTGAGGGTAGCGAGAAGCTCGCTACCGTTCC 
               
               
                   
               
               
                 CTCCGGTGGTGCTGGTGCCGCTGCCCCTGCTGCTGGCGGTGCCGCTGC 
               
               
                   
               
               
                 CGGTGGTGACGCCCCCGCCGCTGAGGAGAAGGAGGAAGAGAAGGAGGA 
               
               
                   
               
               
                 GTCCGATGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 48: 
               
               
                 &gt;AV1323\(AspP2-F) P2 sequences generated for 
               
               
                 A.versicolor 
               
               
                 TCGCCCTTGCTGGCAACGAGAGCCCCTCTGCTTCCGACATTAAGGAGG 
               
               
                   
               
               
                 TTCTGTCCTCCGTCGGTGTTGACGCTGACAACGAGCGCCTCGAGAAGC 
               
               
                   
               
               
                 TCATCGCTGAGCTCCAGGGCAAGGACATCAACGAGGTTCGTTTTGACA 
               
               
                   
               
               
                 GATGCATTTGAAATACTTGGCCAGCAGACTAATGAAACCTCTTCTGCA 
               
               
                   
               
               
                 GTTGATCGCTGAGGGTACCACCAAGCTCGCTTCCGTTCCCTCTGGCGG 
               
               
                   
               
               
                 TGGTGGTGGTGCTGCCGCCCCCGCTGCTGGTGGCGCTGCCGCCGCTGA 
               
               
                   
               
               
                 GGCCCCTGCTGCTGAGAAGGAGGAGGAGAAGGAGGAGTCCGACGAGGA 
               
               
                   
               
               
                 ATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 49: 
               
               
                 &gt;AV2916\(Asp2F) sequences generated for 
               
               
                 A.versicolor 
               
               
                 CCCTTGCTGGCAACGAGAGCCCCTCTGCTTCCGACATTAAGGAGGTTC 
               
               
                   
               
               
                 TGTCCTCCGTCGGTGTTGACGCTGACGACGAGCGCCTCGAGAAGCTCA 
               
               
                   
               
               
                 TCGCTGAGCTCCAGGGCAAGGACATCAACGAGGTTCGTTTTGACAGAT 
               
               
                   
               
               
                 GCGTTTGAAATACTTGGCCAGCAGACTAATGAAACCTCTTCTGCAGTT 
               
               
                   
               
               
                 GATCGCTGAGGGTACCACCAAGCTCGCTTCCGTTCCCTCTGGCGGTGG 
               
               
                   
               
               
                 TGGTGGTGCTGCCGCCCCCGCTGCTGGTGGCGCTGCCGCCGCTGAGGC 
               
               
                   
               
               
                 CCCTGCTGCTGAGAAGGAGGAGGAGAAGGAGGAGTCCGACGAGGACAT 
               
               
                   
               
               
                 GGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 50: 
               
               
                 &gt;Published P2B sequences for  C albicans   
               
               
                 gi|11229041|gb|AF317662.1|AF317662 
               
               
                   Candida albicans  60S acidic ribosomal protein 
               
               
                 type P2-B (p2B) gene, complete cds 
               
               
                 GGAAAAAATGAAATACTTAGCTGCTTACTTATTGTTAGTTCAAGGTGG 
               
               
                   
               
               
                 TAACACCTCTCCATCAGCTTCTGATATCACCGCTTTATTGGAATCCGT 
               
               
                   
               
               
                 TGGTGTTGAAGCCGAAGAATCCAGATTACAAGCTTTATTGAAAGATTT 
               
               
                   
               
               
                 GGAAGGTAAAGACTTGCAAGAATTGATTGCTGAAGGTAACACCAAATT 
               
               
                   
               
               
                 AGCTTCTGTCCCATCCGGTGGTGCTGCTGCTGGTGGTGCTTCTGCCTC 
               
               
                   
               
               
                 TACTGGTGCCGCTGCTGGTGGTGCTGCCGAAGCTGAAGAAGAAAAAGA 
               
               
                   
               
               
                 AGAAGAAGCCAAAGAAGAATCTGATGATGATATGGGTTTCGGTTTATT 
               
               
                   
               
               
                 CGATTAGAGAAATTGCTTGTCGCCTTTGCTGGTTTGAGAGAAGTATAT 
               
               
                   
               
               
                 TTCCATTATTTTGCATTATATATATATATATGTATTATAACTAATCTA 
               
               
                   
               
               
                 ATAAAAAAAATATGAAAACAAAAATGGCTTCTATATGGCACTGTTTGC 
               
               
                   
               
               
                 A 
               
               
                   
               
               
                 SEQ ID NO 51: 
               
               
                 &gt;Published P2B sequences for  C . glabrata   
               
               
                 gi|50284952|ref|XM_444905.1|  Candida   
               
               
                   glabrata  CBS138 hypothetical protein  
               
               
                 (CAGLOA03168g) partial mRNA 
               
               
                 ATGAAGTACTTGGCCGCTTACCTATTGTTGACCCAAGGTGGTAACGAA 
               
               
                   
               
               
                 TCTCCAGCTGCTGCTGACATCAAGAAGGTTATCGAATCTGTTGGTATT 
               
               
                   
               
               
                 GAAGCTGACGAAGCTAGAATCAACGAATTGTTGTCTGCTTTGGAAGGT 
               
               
                   
               
               
                 AAGTCCTTGGACGAATTGATCGCTGAAGGTCAACAAAAGTTCGCCTCT 
               
               
                   
               
               
                 GTTCCAGTTGGTGGTGCTGCTGCTGGTGGTGCTTCCGCTGCTGCTGGT 
               
               
                   
               
               
                 GGTGCCGCTGCCGGTGAAGCTGCTGAAGAAAAGGAAGAAGAAGCTGCT 
               
               
                   
               
               
                 GAAGAATCCGATGACGACATGGGTTTCGGTTTGTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 52: 
               
               
                 &gt;gi|68465556|ref|XM_718047.1|  Candida   albicans   
               
               
                 SC5314 cytosolic ribosomal acidic protein P2B 
               
               
                 (CaO19_5928) partial mRNA 
               
               
                 ATGAAATACTTAGCTGCTTACTTATTGTTAGTTCAAGGTGGTAACACC 
               
               
                   
               
               
                 TCTCCATCAGCTTCTGATATCACCGCTTTATTGGAATCCGTTGGTGTT 
               
               
                   
               
               
                 GAAGCCGAAGAATCCAGATTACAAGCTTTATTGAAAGATTTGGAAGGT 
               
               
                   
               
               
                 AAAGACTTGCAAGAATTGATTGCTGAAGGTAACACCAAATTAGCTTCT 
               
               
                   
               
               
                 GTCCCATCCGGTGGTGCTGCTGCTGGTGGTGCTTCTGCCTCTGCTGGT 
               
               
                   
               
               
                 GCCGCTGCTGGTGGTGCTGCTGAAGCTGAAGAAGAAAAAGAAGAAGAA 
               
               
                   
               
               
                 GCCAAAGAAGAATCTGATGATGATATGGGTTTCGGTTTATTCGATTAG 
               
               
                   
               
               
                 SEQ ID NO 53: 
               
               
                 &gt;gi|68465849|ref|XM_717900.1|  Candida albicans   
               
               
                 SC5314 cytosolic ribosomal acidic protein P2B 
               
               
                 (CaO19_13349) partial mRNA 
               
               
                 ATGAAATACTTAGCTGCTTACTTATTGTTAGTTCAAGGTGGTAACACC 
               
               
                   
               
               
                 TCTCCATCAGCTTCTGATATCACCGCTTTATTGGAATCCGTTGGTGTT 
               
               
                   
               
               
                 GAAGCCGAAGAATCCAGATTACAAGCTTTATTGAAAGATTTGGAAGGT 
               
               
                   
               
               
                 AAAGACTTGCAAGAATTGATTGCTGAAGGTAACACCAAATTAGCTTCT 
               
               
                   
               
               
                 GTCCCATCCGGTGGTGCTGCTGCTGGTGGTGCTTCTGCCTCTGCTGGT 
               
               
                   
               
               
                 GCCGCTGCTGGTGGTGCTGCTGAAGCTGAAGAAGAAAAAGAAGAAGAA 
               
               
                   
               
               
                 GCCAAAGAAGAATCTGATGATGATATGGGTTTCGGTTTATTCGATTAG 
               
               
                   
               
               
                 SEQ ID NO 54: 
               
               
                 &gt;Published P2 sequence for  A . fumigatus   
               
               
                 gi|71001323|ref|XM_750250.1|  Aspergillus fumigatus   
               
               
                 Af293 60S acidic ribosomal protein P2/allergen 
               
               
                 Asp F 8 (AFUA_2G10100) mRNA, complete cds 
               
               
                 ATGAAGCACCTCGCCGCTTACCTCCTCCTCGCCCTTGCTGGCAACACC 
               
               
                   
               
               
                 TCCCCGTCCTCTGAGGATGTCAAGGCCGTCCTCTCTTCCGTTGGCATT 
               
               
                   
               
               
                 GATGCCGATGAGGAGCGCCTGAACAAGCTCATTGCTGAGCTCGAGGGC 
               
               
                   
               
               
                 AAGGACCTCCAGGAGCTCATCGCCGAGGGTTCCACCAAGCTCGCTTCC 
               
               
                   
               
               
                 GTTCCCTCCGGTGGTGCTGCCGCCGCTGCTCCTGCCGCTGCCGGTGCC 
               
               
                   
               
               
                 GCTGCCGGTGGTGCTGCTGCTCCTGCCGCTGAGGAGAAGAAGGAGGAG 
               
               
                   
               
               
                 GAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCTTTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 55: 
               
               
                 Published P2 sequence for  N . fischeri   
               
               
                 gi|119480930|ref|1XM_001260493.1| Neosartorya   
               
               
                   fischeri  NRRL 181 60S acidic ribosomal protein 
               
               
                 P2/allergen Asp F 8 (NFIA_085510) mRNA, complete 
               
               
                 cds 
               
               
                 ATGAAGCACCTCGCCGCTTACCTCCTCCTCGCCCTTGCTGGCAACACC 
               
               
                   
               
               
                 TCCCCGTCCGCTGAGGATGTCAAGGCCGTCCTCTCTTCCGTTGGCATT 
               
               
                   
               
               
                 GACGCCGATGAGGAGCGCCTGAACAAGCTCATTGCTGAGCTCGAGGGC 
               
               
                   
               
               
                 AAGGACCTCCAGGAGCTCATCGCCGAGGGTTCCACCAAGCTCGCTTCC 
               
               
                   
               
               
                 GTTCCCTCCGGTGGTGCTGCCGCCGCTGCTCCTGCCGCTGGCGGTGCC 
               
               
                   
               
               
                 GCTGCCGGTGGTGCTGCTGCTCCTGCCGCTGAGGAGAAGAAGGAGGAG 
               
               
                   
               
               
                 GAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCTCTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 56: 
               
               
                 Published P2 sequence for  A . clavatus   
               
               
                 gi|121715403|ref|XM_001275310.1|  Aspergillus   
               
               
                   clavatus  NRRL 1 60S acidic ribosomal protein P2, 
               
               
                 putative (ACLA_069130) mRNA, complete cds 
               
               
                 ATGAAGCACCTCGCCGCTTACCTCCTCCTCGCCCTTGGTGGCAACGCC 
               
               
                   
               
               
                 AGCCCCTCCGCTGCTGATGTTAAGGAGGTTCTCTCTTCTGTCGGCATT 
               
               
                   
               
               
                 GATGCTGATGAGGAGCGCCTCAACAAGCTCATTGCTGAGCTCGAGGGC 
               
               
                   
               
               
                 AAGGACCTTCAGGAGCTGATTGCTGAGGGTTCCACCAAGCTCGCTTCC 
               
               
                   
               
               
                 ATTCCCTCCGGCGGTGCTGGTGGTGCTGCCCCCGCCGCTGGCGGTGCT 
               
               
                   
               
               
                 GCCGCCGGTGGTGCTGCTGAGGCCGCTCCCGCTGAGGAGAAGGAGGAG 
               
               
                   
               
               
                 GAGAAGGAGGAGTCCGACGACGACATGGGCTTCGGTCTCTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 57: 
               
               
                 Published P2 sequence for  A . terreus  gi|115395965| 
               
               
                 ref|XM_001213622.1|  Aspergillus terreus  NIH2624 
               
               
                 predicted protein (ATEG_04444) mRNA, complete cds 
               
               
                 ATGAAGCACCTCGCCGCTTTCCTTCTCCTCGGCCTTGCCGGCAACACC 
               
               
                   
               
               
                 TCCCCCTCTGCTGAGGACATCAAGGCTGTCCTCTCCTCCGTCGGCATT 
               
               
                   
               
               
                 GACGCTGATGAGGAGCGCCTCGGCCAGCTCCTGAAGGAGCTCGAGGGC 
               
               
                   
               
               
                 AAGGACATCCAGGAGCTCATCGCTCAGGGCTCTGAGAAGCTCGCCTCC 
               
               
                   
               
               
                 GTTCCCTCTGGCGGTGCTGCCGCTGGTGCTGCTGCTCCCGCCGCTGCC 
               
               
                   
               
               
                 GCTGGCGGTGACGCTGCTGCCCCCGCTGAGAAGAAGGAGGAGGAGAAG 
               
               
                   
               
               
                 GAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCTCTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 58: 
               
               
                 Published P2 sequence for  A .  niger  gi|145252575| 
               
               
                 ref|XM_001397764.1|  Aspergillus niger  CBS 513.88 
               
               
                 hypothetical protein (An16g04930) mRNA, complete 
               
               
                 cds 
               
               
                 ATGAAGTACCTCGCCGCCTACCTTCTGTTGGCCCTTGCTGGCAACAAC 
               
               
                   
               
               
                 ACCCCCTCCGCTGAGGACATCAAGTCCGTCCTCTCCGCCGTCGGCATT 
               
               
                   
               
               
                 GACGCTGAGGAGGAGCGCCTCCAGAAGCTCCTTGCTGAGCTTGAGGGC 
               
               
                   
               
               
                 AAGGACCTCCAGGAGCTCATCTCCGAGGGTACCCAGAAGCTCGCTTCC 
               
               
                   
               
               
                 GTTCCCTCCGGTGGTGCCGGTGCTGCTGCCGCTGCCCCCGCTGCCGGT 
               
               
                   
               
               
                 GGCGCCGCTGCTGCTGAGGCTCCCGCTGAGGAGAAGAAGGAGGAGGCT 
               
               
                   
               
               
                 GCTGAGGAGTCCGATGAGGACATGGGCTTCGGTCTCTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 59: 
               
               
                 &gt;gi|67539651|ref|XM_658508.1| Aspergillus   
               
               
                   nidulans  FGSC A4 chromosomel 
               
               
                 ATGAAGCACCTCGCAGCCTACCTCCTCCTCGCCCTTGCTGGCAACGAG 
               
               
                   
               
               
                 TCTCCCTCCGCCTCCGACATCAAGGAGGTTCTCTCTTCCGTCGGTGTT 
               
               
                   
               
               
                 GACGCCGACGACGAGCGCCTCGAGAAGCTCATTGCTGAGCTCCAGGGC 
               
               
                   
               
               
                 AAGGACATCAACGAGCTGATCGCTGAGGGTACCACCAAGCTTGCCTCC 
               
               
                   
               
               
                 GTTCCCTCCGGCGGTGCTGGTGGTGCTGCCCCTGCTGCCGCTGCCGGT 
               
               
                   
               
               
                 GGTGCTGCTGCTGCCGAGGCCCCCGCTGCTGAGAAGGAGGAGGAGAAG 
               
               
                   
               
               
                 GAGGAGTCCGATGAGGACATGGGCTTCGGTCTCTTCGACTAA 
               
               
                   
               
               
                 SEQ ID NO 60: 
               
               
                 &gt;gi|11229039|gb|AF317661.1|  Candida albicans   
               
               
                 60S acidic ribosomal protein type P2-A (p2A) 
               
               
                 gene, complete cds 
               
               
                 GAATTCGATTATTGCATTCTGATATTCCCTGCTTTAAATGCATTTGGA 
               
               
                   
               
               
                 AATATTTCGTATATCATGAGATATAATAACATTAATAGCATTTTCATG 
               
               
                   
               
               
                 TTACTAACAAGAATATA GTGAAATACTTAGCTGCTTAC TTATTATTAG 
               
               
                   
               
               
                 TTAACGCCGGTAACGCCACCCCATCTGCTGCCGATGTCAAAGCTGTTT 
               
               
                   
               
               
                 TGTCAGCTGCTGATATTGAAGTCGAAGAAGAAAAAGTTGAAAAATTGA 
               
               
                   
               
               
                 TCAGCGAATCGGACGGTAAGAACGTCGAAGAATTGATTGCTGAAGGTA 
               
               
                   
               
               
                 ACGAAAAATTATCATCAGTCCCATCTGGTGCTCCAGCTGCTGCTGCTG 
               
               
                   
               
               
                 GTGGTGCTTCTGCTGCCGCCGGTGGTGAAGCCACTGAAGAAGCTGCTG 
               
               
                   
               
               
                 AAGAAGAAGCTGCTGAAGAATCTGATGACGATA TGAGTTTCGGTTTAT   
               
               
                   
               
               
                   TCGATTA AACGAGTCAACAGGCATCTCAAGATCACAGCATAAGG 
               
               
                   
               
               
                   A .  fumigatus   
               
               
                 SEQ ID NO 110: 
               
               
                 &gt;A. FUM505.62 
               
               
                 TTACCTCCTCCTCGCCCTTGCTGGCAACACCTCCCCGTCCTCTGAGGA 
               
               
                   
               
               
                 TGTCAAGGCCGTCCTCTCTTCCGTTGGCATTGATGCCGATGAGGAGCG 
               
               
                   
               
               
                 CCTGAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 TAGTAACTACAGCTCGAAGATTACAGACTGGGAATTTTGGACTGGCGC 
               
               
                   
               
               
                 TGACATCGAACTCTACAACAGCTCATTGCCGAGGGTTCCACCAAGCTC 
               
               
                   
               
               
                 GCTTCCGTTCCCTCCGGTGGTGCTGCCGCCGCTGCTCCTGCCGCTGCC 
               
               
                   
               
               
                 GGTGCCGCTGCCGGTGGTGCTGCTGCTCCTGCCGCTGAGGAGAAGAAG 
               
               
                   
               
               
                 GAGGAGGAGAAGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                   A .  flavus   
               
               
                 SEQ ID NO 61: 
               
               
                 &gt; A . flavus 2199\(P2) 
               
               
                 TCGCCCTCGCTGGCAACTCCACCCCCTCCGTTGAGGACATCAAGAGCG 
               
               
                   
               
               
                 TTCTCTCTTCCGTCGGTATTGATGCCGATGAGGAGCGCCTCCAGAAGG 
               
               
                   
               
               
                 TCATCTCCGAGCTCGAGGGCAAGGACCTCCAGCAGGTTCGTAATACGT 
               
               
                   
               
               
                 TGAATGATTGAGACATAGGGCGCCTGCTGACCATCTACTCACTACAGC 
               
               
                   
               
               
                 TGATCACTGAGGGTAGCGAGAAGCTCGCTACCGTTCCCTCCGGTGGTG 
               
               
                   
               
               
                 CTGGTGCCGCTGCCCCTGCTGCTGGCGGTGCCGCTGCCGGTGGTGACG 
               
               
                   
               
               
                 CCCCCGCCGCTGAGGAGAAGGAGGAAGAGAAGGAGGAGTCCGATGAGG 
               
               
                   
               
               
                 ACATGGGCTTCGGTCT 
               
               
                   
               
               
                   A .  niger   
               
               
                 SEQ ID NO 62: 
               
               
                 &gt;A. nig2828\(P2F) 
               
               
                 CTACCTTCTGTTGGCCCTTGCTGGCAACAACACCCCCTCCGCTGAGGA 
               
               
                   
               
               
                 CATCAAGTCCGTCCTCTCCGCCGTCGGCATTGACGCTGAGGAGGAGCG 
               
               
                   
               
               
                 CCTCCAGAAGCTCCTTGCTGAGCTTGAGGGCAAGGACCTCCAGGAGGT 
               
               
                   
               
               
                 CAGTTAACGCCCTTAAAAATCTACCAAGAAATTTTGCGATTACAAGAT 
               
               
                   
               
               
                 GGAATACTGACAATGGTTTTTTCTACAACAGCTCATCTCCGAGGGTAC 
               
               
                   
               
               
                 CCAGAAGCTCGCTTCCGTTCCCTCCGGTGGTGCCGGTGCTGCTGCCGC 
               
               
                   
               
               
                 TGCCCCCGCTGCCGGTGGCGCCGCTGCTGCTGAGGCTCCCGCTGAGGA 
               
               
                   
               
               
                 GAAGAAGGAGGAGGCTGCTGAGGAGTCCGATGAGGACATGGGCTTCGG 
               
               
                   
               
               
                 TCT 
               
               
                   
               
               
                   A .  terreus   
               
               
                 SEQ ID NO 63: 
               
               
                 &gt;A. terr307 
               
               
                 TTTCCTTCTCCTCGGCCTTGCCGGCAACACCTCCCCCTCTGCTGAGGA 
               
               
                   
               
               
                 CATCAAGGCTGTCCTCTCCTCCGTCGGCATTGACGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCGGCCAGCTCCTGAAGGAGCTCGAGGGCAAGGACATCCAGGAGGT 
               
               
                   
               
               
                 TAGTGATCACCATAACTTCGGACTACTGCGATGAAAACGCGCCATACT 
               
               
                   
               
               
                 AACGGATTATACAGCTCATCGCTCAGGGCTCTGAGAAGCTCGCCTCCG 
               
               
                   
               
               
                 TTCCCTCTGGCGGTGCTGCCGCTGGTGCTGCTGCTCCCGCCGCTGCCG 
               
               
                   
               
               
                 CTGGCGGTGACGCTGCTGCCCCCGCTGAGAAGAAGGAGGAGGAGAAGG 
               
               
                   
               
               
                 AGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 64: 
               
               
                 &gt;A. terr5677 
               
               
                 TTTCCTTCTCCTCGGCCTTGCCGGCAACACCTCCCCCTCTGCTGAGGA 
               
               
                   
               
               
                 CATCAAGGCTGTCCTCTCCTCCGTCGGCATTGACGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCGGCCAGCTCCTGAAGGAGCTCGAGGGCAAGGACATCCAGGAGGT 
               
               
                   
               
               
                 TAGTGATCACCATAACTTCGGACTACTGCGATGAAAACGCGCCATACT 
               
               
                   
               
               
                 AACGGATTATACAGGTCATCGCTCAGGGCTCTGAGAAGCTCGCCTCCG 
               
               
                   
               
               
                 TTCCCTCTGGCGGTGCTGCCGCTGGTGCTGCTGCTCCCGCCGCTGCCG 
               
               
                   
               
               
                 CTGGCGGTGACGCTGCTGCCCCCGCTGAGAAGAAGGAGGAGGAGAAGG 
               
               
                   
               
               
                 AGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 65: 
               
               
                 &gt;A. terr2729 
               
               
                 TTTCCTTCTCCTCGGCCTTGCCGGCAACACCTCCCCCTCTGCTGAGGA 
               
               
                   
               
               
                 CATCAAGGCTGTCCTCTCCTCCGTCGGCATTGACGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCGGCCAGCTCCTGAAGGAGCTCGAGGGCAAGGACATCCAGGAGGT 
               
               
                   
               
               
                 TAGTGATCACCATAACTTCGGACTACTGCGATGAAAACGCGCCATACT 
               
               
                   
               
               
                 AACGGATTATACAGCTCATCGCTCAGGGCTCTGAGAAGCTCGCCTCCG 
               
               
                   
               
               
                 TTCCCTCTGGCGGTGCTGCCGCTGGTGCTGCTGCTCCCGCCGCTGCCG 
               
               
                   
               
               
                 CTGGCGGTGACGCTGCTGCCCCCGCTGAGAAGAAGGAGGAGGAGAAGG 
               
               
                   
               
               
                 AGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 66: 
               
               
                 &gt;A. terr601-65 
               
               
                 TTTCCTTCTCCTCGGCCTTGCCGGCAACACCTCCCCCTCTGCTGAGGA 
               
               
                   
               
               
                 CATCAAGGCTGTCCTCTCCTCCGTCGGCATTGACGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCGGCCAGCTCCTGAAGGAGCTCGAGGGCAAGGACATCCAGGAGGT 
               
               
                   
               
               
                 TAGTGATCACCATAACTTCGGACTACTGCGATGAAAACGCGCCATACT 
               
               
                   
               
               
                 AACGGATTATACAGCTCATCGCTCAGGGCTCTGAGAAGCTCGCCTCCG 
               
               
                   
               
               
                 TTCCCTCTGGCGGTGCTGCCGCTGGTGCTGCTGCTCCCGCCGCTGCCG 
               
               
                   
               
               
                 CTGGCGGTGACGCTGCTGCCCCCGCTGAGAAGAAGGAGGAGGAGAAGG 
               
               
                   
               
               
                 AGGAGGAGTCCGACGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                   A .  candidus   
               
               
                 SEQ ID NO 67: 
               
               
                 &gt;A. Cand 225-8\(P2F) 
               
               
                 TGCTCCTCGGCCTCGCMGGCAACGAGACTCCCTCCGCTGCCGACATCA 
               
               
                   
               
               
                 AGGGCGTTCTGTCCGCCGTCGGCATTGACGCCGATGAGGACCGTCTCT 
               
               
                   
               
               
                 CCAAGCTCCTCTCCGAGCTTGAGGGCAAGGACATCAACGAGGTTCGTA 
               
               
                   
               
               
                 TCTCACAGGAATCGCACACGTAACAGAGTCAACAAATACTAATCCCCC 
               
               
                   
               
               
                 GTGCAGCTGATCGCCCAGGGCTCCGAGAAGCTTGCTTCCGTTCCCTCC 
               
               
                   
               
               
                 GGTGGTGCCGCTGGTGGTGCCGCTGCCGCCCCTGCCGCCGCCGCTGGT 
               
               
                   
               
               
                 GGTGACGCTCCCGCCCAGGAGAAGGAGGAGGAGAAGGAGGAGTCCGAT 
               
               
                   
               
               
                 GAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 68: 
               
               
                 &gt;A. Cand14607\(P2F) 
               
               
                 TGCTCCTCGGCCTCGCCGGCAACGAGTCTCCCTCCGCTGCCGACATCA 
               
               
                   
               
               
                 AGGGCGTTCTGTCCGCCGTCGGCATTGACGCCGATGAGGAGCGTCTCT 
               
               
                   
               
               
                 CCAAGCTCCTCTCCGAGCTTGAGGGCAAGGACATCAACGAGGTTCGTA 
               
               
                   
               
               
                 TCCTGGAATCGCACCGAGACAGAGTCAACAAATACTAATCCCCCGTGC 
               
               
                   
               
               
                 AGCTGATCGCCCAGGGTACCGAGAAGCTTGCTTCCGTTCCCTCCGGTG 
               
               
                   
               
               
                 GCGCCGGCGCTGCTGCTGCCGCCCCTGCCGCCGCTGCTGGTGGTGAGG 
               
               
                   
               
               
                 CCGCCGCTGAGGAGAAGAAGGAGGAGGAGAAGGAGGAGTCCGATGAGG 
               
               
                   
               
               
                 ACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 69: 
               
               
                 &gt;A. Cand-9695\(APF) 
               
               
                 GGCAACCGAGTCTCCCTCCGCTGCCGACATCAAGGGCGTTCTGTCCGC 
               
               
                   
               
               
                 CGTCGGCATTGACGCCGATGAGGAGCGTCTCTCCAAGCTCCTCTCCGA 
               
               
                   
               
               
                 GCTTGAGGGCAAGGACATCAACGAGGTTCGTATCCTGGAATCGCACCG 
               
               
                   
               
               
                 AGACAGAGTCAACAAATACTAATCCCCCGTGCAGCTGATCGCCCAGGG 
               
               
                   
               
               
                 TACCGAGAAGCTTGCTTCCGTTCCCTCCGGTGGCGCCGGCGCTGCTGC 
               
               
                   
               
               
                 TGCCGCCCCTGCCGCCGCTGCTGGTGGTGAGGCCGCCGCTGAGGAGAA 
               
               
                   
               
               
                 GAAGGAGGAGGAGAAGGAGGAGTCCGATGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                   A .  clavatus   
               
               
                 SEQ ID NO 70: 
               
               
                 &gt;A. clav7944\(P2F) 
               
               
                 TTACCTCCTCCTCGCCCTTGGTGGCAACGCCAGCCCCTCCGCTGCTGA 
               
               
                   
               
               
                 TGTTAAGGAGGTTCTCTCTTCCGTCGGCATTGATGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCAACAAGCTCATTGCTGAGCTCGAGGGCAAGGACCTTCAGGAGGT 
               
               
                   
               
               
                 TAGTTTTACGCTGGTCTACGAGAGGAAGATTGTGACAAGATGCTAACG 
               
               
                   
               
               
                 GAAAATTTCTTCAACAGCTGATTGCTGAGGGTTCCACCAAGCTCGCTT 
               
               
                   
               
               
                 CCATTCCCTCCGGCGGTGCTGGTGGTGCTGCCCCCGCCGCTGGCGGTG 
               
               
                   
               
               
                 CTGCCGCCGGTGGTGCTGCTGAGGCCGCTCCCGCTGAGGAGAAGGAGG 
               
               
                   
               
               
                 AGGAGAAGGAGGAGTCCGACGACGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 71: 
               
               
                 &gt;A. clav443\(P2F) 
               
               
                 TTACCTCCTCCTCGCCCTTGGTGGCAACGCCAGCCCCTCCGCTGCTGA 
               
               
                   
               
               
                 TGTTAAGGAGGTTCTCTCTTCCGTTGGCATTGATGCTGATGAGGAGCG 
               
               
                   
               
               
                 CCTCAATAAGCTCATTGCTGAGCTTGAGGGCAAGGACCTTCAGGAGGT 
               
               
                   
               
               
                 TAGTTTACGCCAATCCGCGATATGAAAATTGCGACATGATGCTAACAG 
               
               
                   
               
               
                 GAGAATTTTTCAACAGCTGATTGCTGAGGGTTCCACCAAGCTCGCTTC 
               
               
                   
               
               
                 CATTCCTTCCGGCGGTGCTGCCGGTGCTGCCCCTGCCGCTGGCGCTGC 
               
               
                   
               
               
                 TGCCGGTGGTGCCGCTGAGGCCGCTCCCGCTGAGGAGAAGGAGGAGGA 
               
               
                   
               
               
                 GAAGGAGGAGTCCGACGACGACATGGGCTTCGGTCT 
               
               
                   
               
               
                   A .  glaucus   
               
               
                 SEQ ID NO 72: 
               
               
                 &gt;A. glau2425\(P2F) 
               
               
                 ACACGGTGGACTGGCTGGCCGGAAGAGCCGTTGCTGGTGTAAGTTTTG 
               
               
                   
               
               
                 GTAGGACCGTTCAGCTCCRGAKGTCTTTGGGGACAATGGCGACGAGGG 
               
               
                   
               
               
                 CTGTCGATCGTTAGTGTACATTCCAAGGAAGGTAAAAGAAAAAGAATG 
               
               
                   
               
               
                 CGACATACAGTAAGTTGAGCCACTCTGACGCTGGCAGTCATCGCAGTG 
               
               
                   
               
               
                 GTCATAAGCTGTAGCGAGCGGCTGCTCGATGTTGACCGAGTAGGTGAC 
               
               
                   
               
               
                 GGCTTTGCAGAGACAGTGACCGGAAATAGGCATTTTGGACGAGAGGCA 
               
               
                   
               
               
                 GAATATAATTGGACTATAGACTGACAATGATGATGACGATACTGAGGA 
               
               
                   
               
               
                 CCTTTGGGAGGAATGAAACAGATATTTATACCCTCTCCGACCTCGTGT 
               
               
                   
               
               
                 AGTCCATCTGTCTTTCGCTGTCTCTCGGTGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 73: 
               
               
                 &gt;A. glau542 
               
               
                 GCGTTGGTGCACGACGGTACCAAGGGGACTCTCATACTCCTTAATTCT 
               
               
                   
               
               
                 CTGGCCGGTCCAGTGTCGCGACTCRTATGGAGCACATTAGCAGATATC 
               
               
                   
               
               
                 CCACAGAGCTACCATGTCGTCCAGGCTCTTTGTCTGCTTTGCACATGG 
               
               
                   
               
               
                 CCTTTTCCAACCAGCAGTACCTCCACAGACCCGACGTTCATGCTGTGT 
               
               
                   
               
               
                 GGTATGATGATGCAAGTCGCCATGCAACTTGGTCTTCACCGGCCTTCG 
               
               
                   
               
               
                 CACACTCAGGACTTTASCAAGTTCACAGTGGAGCTGATTGAGGAGGAG 
               
               
                   
               
               
                 CTCAGGGATAAAGTGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 74: 
               
               
                 &gt;A. glaucus29771\(P2) 
               
               
                 GCGTTGGAGCACGACTGTACCAGGATACTCATCATCTCCTTAATTTTC 
               
               
                   
               
               
                 TGGCCGGTCCAGCGYCGCGACTAGTATGGAGCACATTATCAGACATCC 
               
               
                   
               
               
                 GACAGAGCTACCATGTCGTAAAGGCTCTTTGTCTGCTCTGCACATGGC 
               
               
                   
               
               
                 CTTTTCCGACCAGCAGTACCTCCACAGACCCTACKTTTATGCTGTGTG 
               
               
                   
               
               
                 GTATGATGATGCAAGTCGCCATGCAGCTTGGTCTTCACCGGCCTTCGC 
               
               
                   
               
               
                 ACACTCAGGACTTTAGCAAGTTTACAGTGGAGCTGATTGAGGAGGAAC 
               
               
                   
               
               
                 TAAGGGACAAAGTGAGGACATGGGCTTCGGTCT 
               
               
                   
               
               
                 A. versicolor 
               
               
                 SEQ ID NO 75: 
               
               
                 &gt;A.Vers6898 
               
               
                 CGCCCTTGCTGGCAACCAGAGCCCCTCTGCTTCCGATGTTAAGGAGGT 
               
               
                   
               
               
                 TCTCTCCTCCGTCGGTGTTGACGCTGACTCTGAGCGCCTCGAGAAGCT 
               
               
                   
               
               
                 CATCGCTGAGCTCCAGGGCAAGGACATCAACGAGGTTCGTTTTGACAG 
               
               
                   
               
               
                 AACCGCTTGAAATTCTTGGCAGCAGACTAATGAAACATTTTCTGCAGT 
               
               
                   
               
               
                 TGATCGCTGAGGGTACCACTAAGCTCGCTTCCGTTCCCTCTGGCGGTG 
               
               
                   
               
               
                 CTGGTGCTGCTGCTGCTCCCGCTGCTGGTGGCGCTGCTGCCGCTGAGG 
               
               
                   
               
               
                 CCCCTGCCGCCGAGAAGGAGGAGGAGAAGGAGGAGTCCGACGAGGACA 
               
               
                   
               
               
                 TGGGCTTCGGTCT 
               
               
                   
               
               
                 SEQ ID NO 76: 
               
               
                 &gt;lus66\(P2BF) sequence generated for  C .  lusitaniae   
               
               
                 KTATTGTTGGKCAACGCTGGTAACACCGCCCCATCTGCTGCTGACGTC 
               
               
                   
               
               
                 AAGAAGGTCTTGGAATCCGTCTCTATTGAGGTTGAGGACGACAAGGTT 
               
               
                   
               
               
                 GAGAAGTTGTTGGCTGAAGTTGAAGGCAAGAACGTCGAAGAGTTGATT 
               
               
                   
               
               
                 GCCGAGGGTAACGAGAAGTTGTCTTCTGTTCCATCTGGTGCTCCAGCT 
               
               
                   
               
               
                 GCTGCTGGTGCCGCTGCTGCTTCTGGTTCTACTGAGGCTGCTGCTGAA 
               
               
                   
               
               
                 GAGCCACAAGAAGAAGAGAAGGAGGAGTCTGACGACGACATGGGTTTC 
               
               
                   
               
               
                 GGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 77: 
               
               
                 &gt;fam1\(P2BF) sequence generated for  C .  famata   
               
               
                 CTTCTCCATCCGCTTCTGACATCAGTAGTTTATTAGAAACCGTTGGTG 
               
               
                   
               
               
                 CTGAAGCTGACGAAGCTAGAATCAGTGCTTTATTGAAGGACTTAGAAG 
               
               
                   
               
               
                 GTAAGCAAGTCGCTGACTTAATTGCTGAAGGTCAAACCAAGTTGGCTT 
               
               
                   
               
               
                 CCGTTCCAACTGGTGGTGCTGGTGCTGCTGCTGGTGGTGCCGCTGCTG 
               
               
                   
               
               
                 CTTCTGGTGATGCCGGTGCAGCTGCTGCTGAAGAAGAAAAGGAAGAAG 
               
               
                   
               
               
                 AAAAGGAAGAATCCGACGATGACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 78: 
               
               
                 &gt;fam2\(P2BF) sequence generated for  C .  famata   
               
               
                 CTTCTCCATCAGCCTCTGACGTCAGTGCTTTATTAGAAACCGTTGGTG 
               
               
                   
               
               
                 CTGAAGTTGACCAAGGTAGAGTTAGTGCTTTATTGAAGGACTTAGAAG 
               
               
                   
               
               
                 GTAAGCAAGTTGCCGACTTAATTGCTGAAGGTCAAACCAAGTTAGCTT 
               
               
                   
               
               
                 CTGTCCCAACCGGTGGTGCTGCTTCTGCTGGTGGTGCTGCCGCTGCTT 
               
               
                   
               
               
                 CTGGTGCTGCCGGTGCAGCTGCTGTTGAAGAAGAAAAGGAAGAAGAAA 
               
               
                   
               
               
                 AGGAAGAATCCGATGAAGATATGGGTTTCGGTTTATTCGATT 
               
               
                   
               
               
                 SEQ ID NO 79: 
               
               
                 &gt;fam5\(P2BF) sequence generated for  C .  famata   
               
               
                 CTTCTCCATCAGCCTCTGACGTCAGTGCTTTATTAGAAACCGTTGGTG 
               
               
                   
               
               
                 CTGAAGTTGACCAAGGTAGAGTCAGTGCTTTATTGAAGGACTTAGAAG 
               
               
                   
               
               
                 GTAAGCAAGTCGCCGACTTAATTGCTGAAGGTCAAACCAAGTTAGCTT 
               
               
                   
               
               
                 CTGTCCCAACTGGTGGTGCTGCTTCTGCTGGTGGTGCTTCCGCTGCTG 
               
               
                   
               
               
                 CTTCTGGTGATGCCGGTGCAGCTGCTGCTGAAGAAGAAAAGGAAGAAG 
               
               
                   
               
               
                 AAAAGGAAGAATCCGATGAAGATATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 80: 
               
               
                 &gt;haem54\(P2BF) sequence generated for  C .  haemuloni   
               
               
                 TTTCGGTTTATTCGATTAACACTTCCCCAGCTGCCTCTGACATCAAGA 
               
               
                   
               
               
                 AGGTGTTGGAGTCTGTCTCCATCGAGGTTGAGGACGACAAGGTCGAGA 
               
               
                   
               
               
                 AGTTGTTGGCTGAGGTCGAGGGCAAGAACGCCGAGGAGTTGATTGCCG 
               
               
                   
               
               
                 AGGGTAACGAGAAGTTGTCTTCTGTCCCAACTGGTGCTCCAGCTGGTG 
               
               
                   
               
               
                 GTGCTGCCGCTGCTGGTGGTGCTGCTCCAGAGGCTGCTGCTGAGAAGG 
               
               
                   
               
               
                 AAGAGGAGGCCGCTGCCGAGGAGTCTGACGACGACATGGGTTTCGGTT 
               
               
                   
               
               
                 TATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 81: 
               
               
                 &gt;haem55\(P2BF) sequence generated for  C .  haemuloni   
               
               
                 TTATTGTTGGTCAACGCCGGTAACACTTCCCCAGCTGCCTCTGACATC 
               
               
                   
               
               
                 AAGAAGGTGTTGGAGTCTGTCTCCATCGAGGTTGAGGACGACAAGGTC 
               
               
                   
               
               
                 GAGAAGTTGTTGGCTGAGGTCGAGGGCAAGAACGCCGAGGAGTTGATT 
               
               
                   
               
               
                 GCCGAGGGTAACGAGAAGTTGTCTTCTGTCCCAACTGGTGCTCCAGCT 
               
               
                   
               
               
                 GGTGGTGCTGCCGCTGCTGGTGGTGCTGCTCCAGAGGCTGCTGCTGAG 
               
               
                   
               
               
                 AAGGAAGAGGAGGCCGCTGCCGAGGAGTCTGACGACGACATGGGTTTC 
               
               
                   
               
               
                 GGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 82: 
               
               
                 &gt;pul36\(P2BF) sequence generated for 
               
               
                   C .  pulcherrima   
               
               
                 ACACCTCTCCATCCGCCGCCGATGTCAAGAAGGTCTTGGAGTCCGTTT 
               
               
                   
               
               
                 CCATCGAGGTTGAGGAGGACAAGGTCGAGAAGTTGCTCGCTGAGGTCG 
               
               
                   
               
               
                 AGGGCAAGAGCGTCGAGGACTTGATCGCTGAGGGTAACGAGAAGTTGT 
               
               
                   
               
               
                 CTTCTGTCCCAACTGGTGGCCCAGCCGCCGGTGGTGCCGCTGCCGCTG 
               
               
                   
               
               
                 CTGGTGGTGACGCCGCTCCTGCCGAGGAGGCCGCTGAGGAGGCCGCCG 
               
               
                   
               
               
                 AGGAGTCTGACGACGACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 83: 
               
               
                 &gt;pul39\(P2BF) sequence generated for  
               
               
                   C .  pulcherrima   
               
               
                 CATCCGCCGCCGATGTCAAGRAGGTCTTGGAGTCCGTTTCCATCGAGG 
               
               
                   
               
               
                 TTGAGGAGGACAAGATCGAGAAGTTGCTCGCTGAGGTCGAGGGCAAGA 
               
               
                   
               
               
                 GCGTCGAGGACTTGATCGCTGAGGGTAACGAGAAGTTGTCTTCTGTCC 
               
               
                   
               
               
                 CAACTGGTGGCCCAGCCGCCGGTGGTGCCGCTGCCGCTGCCGGTGGTG 
               
               
                   
               
               
                 ACGCCGCTCCTGCCGAGGAGGCCGCTGAGGAGGCCGCCGAGGAGTCTG 
               
               
                   
               
               
                 ACGACGACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 SEQ ID NO 84: 
               
               
                 &gt;U50\(P2BF) sequence generated for  C .  utilis   
               
               
                 ACCGCCGACAAGATCACCTCCGTCTTGGAGTCTGTCGGTATTGAGGTT 
               
               
                   
               
               
                 GAGGAGTCCCAAGTCACCGAGTTGATCTCTGCCCTTGAGGGTAAGTCC 
               
               
                   
               
               
                 GTTGAGGAGCTCATTGCTGAAGGTAACGAGAAGTTGGCTTCTGTTCCA 
               
               
                   
               
               
                 ACCGGTGGTGCTGGTGCTGCTCCAGCTGCCGGTGCCGGTGCTGCTGAT 
               
               
                   
               
               
                 GCTGATGCTCCAGCTGAGGCTGCTGAGGAGGCTGCTGAGGAGGAGTCT 
               
               
                   
               
               
                 GACGATGACATGGGTTTCGGTTTATTCGATTA 
               
               
                   
               
               
                 Seq ID No 85 
               
               
                 P2ForA 
               
               
                 GAGGAGCGCCT 
               
               
                   
               
               
                 Seq ID No 86 
               
               
                 P2ForB 
               
               
                 GAGGAGCGCCTC 
               
               
                   
               
               
                 Seq ID No 87 
               
               
                 P2ForC 
               
               
                 GGAGGAGCGCCTC 
               
               
                   
               
               
                 Seq ID No 88 
               
               
                 P2ForD 
               
               
                 TGAGGAGCGCCTC 
               
               
                   
               
               
                 Seq ID No 89 
               
               
                 P2RevA 
               
               
                 CCGGAGGGAACGGA 
               
               
                   
               
               
                 Seq ID No 90 
               
               
                 P2RevB 
               
               
                 CCGGAGGGAACGG 
               
               
                   
               
               
                 SeqID No 91 
               
               
                 P2FumP 
               
               
                 ACTACAGCTCGAAGATTA 
               
               
                   
               
               
                 Seq ID No 92 
               
               
                 P2FlavP 
               
               
                 ACGTTGAATGATTGAGAC 
               
               
                   
               
               
                 Seq ID No 93 
               
               
                 P2NigP 
               
               
                 TTGCGATTACAAGATGGAA 
               
               
                   
               
               
                 Seq ID No 94 
               
               
                 P2TerrP 
               
               
                 CTTCGGACTACTGCGATGA 
               
               
                   
               
               
                 Seq ID No 95 
               
               
                 CAP2BF (For) 
               
               
                 ACCTCTCCATCAGCTTCTG 
               
               
                   
               
               
                 Seq ID No 96 
               
               
                 CGP2BF (For) 
               
               
                 ACCTCTGTCTTATCATCTGTCG 
               
               
                   
               
               
                 Seq ID No 97 
               
               
                 CGP2BF (For) 
               
               
                 AAGAAGGTTATCGAATCTGTTG 
               
               
                   
               
               
                 Seq ID No 98 
               
               
                 CTP2BF (For) 
               
               
                 TCCGCTTTATTGGAACAAGTTG 
               
               
                   
               
               
                 Seq ID No 99 
               
               
                 CPP2BF (For) 
               
               
                 TCCTCATTGTTGGAATCCGTTG 
               
               
                   
               
               
                 Seq ID 100 
               
               
                 CAP2BR (Rev) 
               
               
                 TCAGCAATCAATTCTTGC 
               
               
                   
               
               
                 SEQ ID 101 
               
               
                 CKP2BR (Rev) 
               
               
                 CTCTTCGACGGACTTACC 
               
               
                   
               
               
                 SEQ ID 102 
               
               
                 CGP2BR (Rev) 
               
               
                 TTCGTCCAAGGACTTACC 
               
               
                   
               
               
                 SEQ ID 103 
               
               
                 CTP2BR (Rev) 
               
               
                 TTCTTGCAAGTCTTTACC 
               
               
                   
               
               
                 SEQ ID 104 
               
               
                 CPP2BR (Rev) 
               
               
                 CTCGTTGATGTCTTTACC 
               
               
                   
               
               
                 SEQ ID 105 
               
               
                 CAP2BP (P) 
               
               
                 ACCGCTTTATTGGAATCCGTTG 
               
               
                   
               
               
                 SEQ ID 106 
               
               
                 CKP2BP (P) 
               
               
                 ATCCGACAAGTTAGACAAGTTAATC 
               
               
                   
               
               
                 SEQ ID 107 
               
               
                 CGP2BP (P) 
               
               
                 AGAATCAACGAATTGTTGTCTGC 
               
               
                   
               
               
                 SEQ ID 108 
               
               
                 CTP2BP (P) 
               
               
                 ATCTTCCAAATTAGACTTATTGTTGA 
               
               
                   
               
               
                 SEQ ID 109 
               
               
                 CPP2BP (P) 
               
               
                 GAAGAATCAAGATTATCTACCTTGTTG 
               
             
          
         
       
     
       References 
       [0075]    Abramczyk D, Tchórzewski M, Krokowski D, Boguszewska A, Grankowski N. Overexpression, purification and characterization of the acidic ribosomal P-proteins from  Candida albicans . Biochim Biophys Acta. 2004 Jun. 11;1672(3):214-23. 
         [0076]    Bailey-Serres J, Vangala S, Szick K, and Lee C H. Acidic phosphoprotein complex of the 60S ribosomal subunit of maize seedling roots. Components and changes in response to flooding. Plant Physiol. 1997 August; 114(4): 1293-1305. 
         [0077]    Newton C H, Shimmin L C, Yee J, Dennis P P. (1990) A family of genes encode the multiple forms of the  Saccharomyces cerevisiae  ribosomal proteins equivalent to the  Escherichia coli  L12 protein and a single form of the L10-equivalent ribosomal protein. JBacteriol. 1990; 172: 579-588 
         [0078]    Tchórzewski M, Krokowski D, Boguszewska A, Liljas A, Grankowski N. Structural characterization of yeast acidic ribosomal P proteins forming the P1A-P2B heterocomplex. Biochemistry. 2003 Apr. 1;42(12):3399-408. 
         [0079]    Tchórzewski M, Boguszewska A, Dukowski P, Grankowski N. Oligomerization properties of the acidic ribosomal P-proteins from  Saccharomyces cerevisiae : effect of P1A protein phosphorylation on the formation of the P1A-P2B hetero-complex. Biochim Biophys Acta. 2000 Dec 11;1499(1-2):63-73. 
         [0080]    Wool I G, Chan Y L, Gluck A, Suzuki K The primary structure of rat ribosomal proteins PO, P1 and P2 and a proposal for a uniform nomenclature for mammalian and yeast ribosomal proteins. Biochimie 1991; 73: 861-870.