Patent Document

Botanical/commercial classification: ‘L514-10’ ( Vitis rufotomentosa ×( V. champinii  ‘Dog Ridge’× V. riparia  ‘Riparia Gloire’))× V. champinii  ‘c9038’. 
     Variety Denomination: ‘9365-85’. 
     BACKGROUND OF THE INVENTION 
     Nematodes are important pests in vineyards around the world, and these soil-borne pests can be particularly problematic in California vineyards. Two factors intensify the impact of nematodes—the high value of grapes and of vineyard land. These factors force growers to ignore the steps of leaving land fallow and rotating crops, both of which reduce nematode build up and delay the selection of adapted strains. Nematicides and fumigants help control nematodes, but the use of these pesticides has been greatly restricted and their future use in doubt because they must be persistent and penetrate deeply through the soil profile to be highly effective. In addition, grape rootstocks were bred to resist grape phylloxera (a devastating root-feeding aphid), and were not selected for nematode resistance. The grape rootstocks ‘Freedom’ and ‘Harmony’ were released to provide resistance to nematodes, but they are not resistant to grape phylloxera. More recently two rootstocks, ‘RS3’ and ‘RS9’ (siblings of a ‘Schwarzmann’ ×‘Ramsey’ cross), were released, which were designed to resist multiple nematode species and provide growers with nematode resistant rootstock alternatives. However, they have nematode resistance from a relatively narrow genetic base, which may promote the evolution of strains capable of feeding on them. Therefore, there is a need to develop nematode resistant grape rootstock. 
     BRIEF SUMMARY OF THE INVENTION 
     This invention relates to a new and distinct variety of grapevine rootstock plant (grape rootstock) with resistance to a broad array of soil-borne nematodes including root-knot, dagger, lesion and citrus nematodes. It is moderately susceptible to ring nematode, but has strong resistance to grape phylloxera. It is a cross of ‘L514-10’ ( Vitis rufotomentosa ×( V. champinii  ‘Dog Ridge’×V. riparia ‘Riparia Gloire’))× V. champinii  ‘c9038’. It is recommended for vineyard sites with severe nematode infestations. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1 : number of ring nematodes recovered off rootstock selections growing in 1 gal pots with three soils, known to have severe and chronic nematode pressure. Results per soils are means of three replicate pots. 
         FIG. 2 : a photograph showing rootstock ‘9365-85’. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     A breeding program was initiated in 1990 at Davis, Calif., to provide a group of rootstocks with broad and durable resistance to the nematodes found in California vineyards. This program commenced with an evaluation of selections that remained from breeding efforts in the late 1960s and early 1970s. These selections from the late 1960s and early 1970s were previously screened against two root knot nematode species ( Meloidogyne incognita acrita  and  M. arenaria thamsei ), the lesion nematode ( Pratylenchus vulnus ), and the dagger nematode ( Xiphinema index ). In 1990, these selections were evaluated for their ability to root and for growth habits such as brushy growth, internode length and the degree of lateral shoot production. In 1993 and 1994, the best of these selections were crossed to species chosen for their ability to reduce scion vigor or improve the rooting of the progeny. The parentage and species composition of the ‘8905-05’ and other crosses are listed in Table 1. About 5,000 progeny were planted in the vineyard and their evaluation for nematode resistance was initiated in 1996. 
     The first phase of the selection process examined the progeny for their general vigor and horticultural characters such as internode length and the degree of lateral shoot formation. The best 1,000 progeny, selected from as many families as possible, were advanced to a rooting assay. In December of 1996, ten 2-node dormant cuttings of the best 1,000 were tested for their ability to form roots. Rootstocks that root well generally graft well, thus this evaluation was a key indicator of their future success as rootstocks. One hundred of the progeny were selected, again with an effort to get a broad representation from the large number of families. 
     The second phase of the selection process involved testing these 100 selections for resistance to  M. incognita  I3. This root-knot nematode isolate is capable of feeding on many rootstocks, but does not feed on rootstocks that derive their resistance from  V. champinii  (‘Freedom’, ‘Harmony’, ‘Dog Ridge’ and ‘Ramsey’). All of the nematode testing in this breeding program utilized potted plants under greenhouse conditions with optimized soils and irrigation techniques to promote nematode feeding. Resistance to this isolate of root-knot nematode was evaluated by assaying the number of galls on the roots after inoculation with 1,000 J2 larvae (the free-living infectious stage of this nematode). Resistance was also evaluated by extracting the J2 nematodes in the pots after the root galls were counted. Later phases of the root-knot nematode screening evaluated resistance by counting the number of egg masses formed using a technique developed by Cousins and Walker (Cousins and Walker 2001 Plant Disease 85:1052-1054). There were no root galls on 33 of the 100 selections and the 33 selections were advanced to the next phase of screening. 
     The third phase of the selection process tested the 33 root-knot nematode resistant selections against two aggressive strains of root-knot nematode and against the dagger nematode,  X. index . The two aggressive strains were selected from soils from a declining vineyard of ‘Harmony’ rootstock. Root-knot nematodes were extracted from this soil and the larvae were placed onto tomato plants with high susceptibility to root-knot nematodes. Two egg masses were collected from these infested plants and J2 larvae from each mass were put on a separate uninfested tomato plant to create new strains from a single egg mass (root-knot nematodes are parthenogenic). These new strains were multiplied on tomato and were then used to inoculate potted plants of ‘Harmony’ rootstock to verify their ability to feed aggressively on this resistant rootstock. These two strains were named HarmA and HarmC. Later investigations determined that HarmA was a strain of  M. arenaria  while HarmC was a strain of  M. incognita . The dagger nematodes were collected from several vineyards in the Napa Valley of California, which were known to have fanleaf degeneration. 
     The 33 selections were then inoculated with each of the three nematode strains independently. The root-knot nematode screens used 1,000 J2 larvae to inoculate plants growing in 1,000 cm 3  plastic pots with a coarse sand/clay loam soil mix. The dagger nematode screens were done in the same pots and soils, but used 200 adult  X. index  as the inoculum. Fourteen selections did not produce egg masses when inoculated with the three root-knot nematode strains nor did they produce root tip galls after inoculation with  X. index  (Table 2). 
     In the fourth phase, the 14 selections were subjected to a series of tests. The first was a combined inoculum of all four nematodes at once to determine the impact of simultaneous nematode feeding on resistance (Table 3). They were also tested against the four nematodes over a range of temperatures, 24, 27, 30 and 32° C. There was some erosion of resistance to HarmA (the most aggressive strain of root-knot nematode) at this temperature, but 6 of the selections performed very well (Table 4). Root-knot nematode resistance is known to fail at about 28° C. in a wide range of species including tomato, pepper and plum. The 14 selections were also evaluated for resistance to lesion ( Pratylenchus vulnus ), citrus ( Tylenchulus semipenetrans ) and ring ( Mesocriconema xenoplax ) nematodes (Table 3). From this series of tests a number of six rootstock selections were made, including ‘9365-85’. A summary of the ‘9365-85’ characteristics is presented below. 
     Grape phylloxera are capable of feeding and producing galls (nodosities) on the young roots of virtually all grape rootstocks and species. In order to gauge the phylloxera hosting ability of the final six selections, they were tested for the ability to support phylloxera on nodosities and compared to a set of commercial rootstocks. Three sets of young root pieces from each selection were inoculated with 10 phylloxera eggs collected from ‘101-14 Mgt’ rootstock roots. Over a 21-day period, the number of eggs and juveniles that were produced were summed and divided by 10 (the original inoculum) to produce the average rate of increase. Table 5 presents these results for the six selections. Nodosity galling on young roots does not appear to damage grapevines, only feeding and galling on mature roots (tuberosities) leads to vine death. The high rate of phylloxera feeding, galling and reproduction on ‘101-14 Mgt’ (7.98 average rate of increase) has been observed in past tests. Most of the values were very low, although this ‘101-14 Mgt’ strains is well adapted to ‘9407-14’. Three of the selections were very resistant: ‘8909-05’, ‘9363-16’, and ‘9449-27’. The low values for AXR#1 demonstrate that the results of this test do not reflect field level or tuberosity level feeding and damage, and that phylloxera adapt independently to rootstock hosts. 
     It will take years to determine which sites each of these rootstock selections are best suited to, but they have unparalleled levels of resistance to nematodes and should excel in sites with single and mixed nematode species infestations. Four of the selections (‘9365-43’, ‘9365-85’, ‘9407-14’ and ‘9449-27’) were grafted to ‘Fiesta Seedless’ and planted in a ‘Fresno’ rootstock trial in 2004. That year two of these selections (‘9365-43’ and ‘9365-85’) were also included in a ‘Chardonnay’ rootstock trial in Santa Maria, Calif. The six rootstock selections in large pots using soils with nematode pressure from root-knot, lesion, ring and  Xiphinema americanum , is known to be severe and chronic. This test was conducted to evaluate these selections under “field conditions” using infested soil without added inoculations. All of the selections performed very well against root-knot nematodes (Table 6) and two, ‘9407-14’, and ‘8909-05’, also performed very well against ring nematode ( FIG. 1 ). 
     Each of the selections was repeatedly asexually reproduced to provide testing materials for nematode resistance evaluations. The cuttings were taken from the original seedling vine planted in Davis, Calif. On Jun. 7, 2002 the original seedlings were asexually reproduced to provide more cuttings for greenhouse and eventual field testing. The variety ‘9365-85’ was first asexually reproduced for distribution in Davis, Calif. from woody and herbaceous cuttings. 
     Detailed Botanical Description 
     Rootstock ‘9365-85’ is a staminate flowered vine with leaves that appear more like  V. riparia  with longer teeth, more prominent lobing, and thinner more puckered texture. Its resistance to root-knot and dagger nematodes in the combined testing was very good, and it resists citrus and lesion nematode, but is susceptible to ring nematode (Table 3). The resistance of ‘9365-85’ to  M. arenaria  HarmA was the most severely impacted by higher temperatures compared with the other five selections, although it is not statistically different from ‘9365-43’ and ‘9363-16’ (Table 4). ‘9365-85’ is an excellent mother vine with long canes, good internode lengths and few lateral shoots. Preliminary propagation results indicate it has moderately deep rooting angles. 
     The following is a further description of rootstock ‘9365-85’: 
     Shoot tips.—The variety has shoot tips which are e enclosed in expanding leaves, typical and observed color - green (5GY ⅚), and sparsely covered with arachnose tomentum. 
     Young leaves.—The variety has young leaves with typical and observed color green (5GY ⅚), and typical and observed shape - three lobed glabrous leaves with fine arachnose tomentum and relatively long sharply pointed teeth. 
     Mature leaves.—The variety has a typical and observed size—medium sized (8.7 ×11 cm), typical and observed upper surface color - dark green upper (5GY 4/4) and typical and observed lower surface color - dark green (5GY 5/4), slightly 3-lobed, broad cuneiform-shaped leaves with medium-sized angular to convex teeth, and medium-sized petioles. The upper surface is glabrous, broadly rugose with some indented puckering near the petiole. The lower surface has scattered arachnose tomentum, fine bristles along the main vines and small tufted bristles (domatia) at the intersection of the main veins. The petiolar sinus is a narrow U to lyre shape.
               Petioles.— typical and observed length 4 to 5 cm, typical and observed diameter—3 to 4 mm, and color is similar to the upper leaf surface (5GY 4/4).     Canes.—typical and observed color.— dark brown (5YR ⅝), typical and observed internodes length—15 to 20 cm, and relatively limited lateral development, nodes have short pointed buds. Canes are oval in cross-section, have thin diaphragms and medium sized pith.     Flowers.— typical and observed flowers are staminate and do not produce berries.           

     
       
         
               
             
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Parentage of the five nematode resistant grape rootstock selections. 
               
             
          
           
               
                   
                 Selection 
                 Parentage 
               
               
                   
               
               
                   
                 8909-05 
                   V. rupestris  ×  M. rotundifolia   
               
               
                   
                 9363-16 
                 ( V. rufotomentosa  × ( V. champinii  ‘Dog Ridge’ × 
               
               
                   
                   
                 Riparia Gloire)) × Riparia Gloire 
               
               
                   
                 9365-43 
                 ( V. rufotomentosa  × ( V. champinii  ‘Dog Ridge’ ×  
               
               
                   
                   
                 Riparia Gloire)) ×  V. champinii  c9038 
               
               
                   
                   
                 (probably  V. candicans  ×  V. monticola ) 
               
               
                   
                 9365-85 
                 ( V. rufotomentosa  × ( V. champinii  ‘Dog Ridge’ ×  
               
               
                   
                   
                 Riparia Gloire)) ×  V. champinii  c9038  
               
               
                   
                   
                 (probably  V. candicans  ×  V. monticola ) 
               
               
                   
                 9407-14 
                 ( V. champinii  ‘Ramsey’ × Riparia Gloire ) ×    
               
               
                   
                   
                   V. champinii  c9021 (probably  V. candicans  × 
               
               
                   
                   
                   V. berlandieri ) 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Selections with broad resistance to four 
               
               
                 nematodes when inoculated individually. 
               
             
          
           
               
                   
                   
                 
                   M. 
                 
                 
                   M. 
                 
                 
                   M. 
                 
               
               
                   
                   
                 
                   incognita  
                 
                   arenaria - 
                   incognita - 
               
               
                 Selection 
                 
                   X. index 
                 
                 I3  
                 HarmA 
                 HarmC 
               
               
                   
               
               
                 8909-05 
                 R 
                 R 
                 R 
                 R 
               
               
                 9317-06 
                 R 
                 R 
                 R 
                 R 
               
               
                 9332-43 
                 R 
                 R 
                 R 
                 R 
               
               
                 9344-03 
                 R 
                 R 
                 R 
                 R 
               
               
                 9363-16 
                 R 
                 R 
                 R 
                 R 
               
               
                 9365-43 
                 R 
                 R 
                 R 
                 R− 
               
               
                 9365-62 
                 R 
                 R 
                 R 
                 R 
               
               
                 9365-85 
                 R− 
                 R 
                 R 
                 R 
               
               
                 9403-35 
                 R 
                 R 
                 R− 
                 R− 
               
               
                 9403-107 
                 R 
                 R 
                 R 
                 R 
               
               
                 9407-14 
                 R 
                 R 
                 R 
                 R 
               
               
                 9449-23 
                 R 
                 R 
                 R 
                 R 
               
               
                 9449-25 
                 R 
                 R 
                 R 
                 R 
               
               
                 9449-27 
                 R 
                 R 
                 R 
                 R 
               
               
                 Control Group: 
                   
                   
                   
                   
               
               
                 1616C 
                 S 
                 R 
                 R− 
                 R− 
               
               
                 Harmony 
                 S 
                 R 
                 S 
                 S 
               
               
                 Colombard 
                 S 
                 S 
                 S 
                 S 
               
               
                   
               
               
                 R = Resistant, no gall symptoms or egg masses observed 
               
               
                 R− = Trace infection 
               
               
                 S = Susceptible, symptoms present, nematode reproduction supported 
               
             
          
         
       
     
     
       
         
               
             
               
               
               
             
               
               
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
               
                   
               
               
                 Summary results for 14 nematode resistant selections when tested 
               
               
                 against combined inoculum with three 
               
               
                 root-knot nematode (RKN) strains and dagger nematode (Xi).  
               
               
                 Results of testing against citrus, lesion and ring are also reported. 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Xi Galls in Combined  
                 RKN Egg Masses in 
               
               
                 Genotypes 
                 Testing 
                 Combined Testing 
               
               
                   
               
             
          
           
               
                 8909-05 
                 R 
                   0 
                 R 
                   0 
               
               
                 9317-06 
                 MS 
                 &lt;1 
                 S 
                 &lt;5 
               
               
                 9332-43 
                 S 
                 &lt;5 
                 S 
                 &lt;5 
               
               
                 9344-03 
                 S 
                 &lt;5 
                 MS 
                 &lt;1 
               
               
                 9363-16 
                 R 
                   0 
                 R 
                   0 
               
               
                 9365-43 
                 R 
                   0 
                 R− 
                 &lt;1 
               
               
                 9365-62 
                 MS 
                 &lt;1 
                 S 
                 &lt;5 
               
               
                 9365-85 
                 MS 
                 &lt;1 
                 R− 
                 &lt;1 
               
               
                 9403-107 
                 R 
                 0 
                 S 
                 &lt;5 
               
               
                 9403-35 
                 S 
                 &lt;5 
                 S 
                 &lt;5 
               
               
                 9407-14 
                 R 
                   0 
                 R 
                   0 
               
               
                 9449-23 
                 MS 
                 &lt;1 
                 R 
                   0 
               
               
                 9449-25 
                 MS 
                 &lt;1 
                 R 
                   0 
               
               
                 9449-27 
                 MS 
                 &lt;1 
                 R− 
                 &lt;1 
               
               
                 1616C 
                 S 
                 &gt;50  
                 S 
                 &lt;5 
               
               
                 Freedom 
                 S 
                 &gt;10  
                 S 
                 &lt;10  
               
               
                 Colombard 
                 S 
                 &gt;100  
                 S 
                 &gt;100  
               
               
                 Harmony 
                 S 
                 &gt;10  
                 S 
                 &lt;50  
               
               
                 St. George 
                 S 
                 &gt;100  
                 S 
                 &lt;50  
               
               
                   
               
             
          
           
               
                 Genotypes 
                 Citrus 
                 Lesion 
                 Ring 
               
               
                   
               
             
          
           
               
                 8909-05 
                 R 
                 &lt;100 
                 R 
                 &lt;10 
                 R 
                  &lt;1,000 
               
               
                 9317-06 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 HS 
                 &lt;20,000 
               
               
                 9332-43 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                 9344-03 
                 S 
                 &gt;400 
                 S 
                 &lt;200  
                 S 
                 &lt;10,000 
               
               
                 9363-16 
                 S 
                 &gt;400 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                 9365-43 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 MS 
                  &lt;5,000 
               
               
                 9365-62 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 MS 
                  &lt;5,000 
               
               
                 9365-85 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                 9403-107 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 MS 
                  &lt;5,000 
               
               
                 9403-35 
                   
                   
                 R 
                 &lt;50 
                 MS 
                  &lt;5,000 
               
               
                 9407-14 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 LS 
                  &lt;3,000 
               
               
                 9449-23 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                 9449-25 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                 9449-27 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                 1616C 
                 S 
                 &gt;400 
                 S 
                 &lt;200  
                 HS 
                 &lt;20,000 
               
               
                 Freedom 
                 R 
                 &lt;100 
                 R 
                 &lt;50 
                 HS 
                 &lt;20,000 
               
               
                 Colombard 
                 S 
                 &lt;1000  
                 S 
                 &lt;300  
                 HS 
                 &lt;30,000 
               
               
                 Harmony 
                 S 
                 &lt;1000  
                 S 
                 &lt;200  
                 HS 
                 &lt;30,000 
               
               
                 St. George 
                 S 
                 &gt;400 
                 R 
                 &lt;50 
                 S 
                 &lt;10,000 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Number of egg masses per potted plant and per 
               
               
                 gram of root after inoculation with 1,000 
               
               
                   M. arenaria  HarmA nematodes and testing at 32° C. 
               
             
          
           
               
                   
                   
                 Egg 
                 Egg Mass/g 
               
               
                   
                 Genotype 
                 Mass/Plant 
                 Root 
               
               
                   
               
             
          
           
               
                   
                 Colombard 
                 442.50 
                 a 
                 155.86 
                 a 
               
               
                   
                 Harmony 
                 156.00 
                 b 
                 123.28 
                 b 
               
               
                   
                 9365-85 
                 32.25 
                 c 
                 16.49 
                 c 
               
               
                   
                 9365-43 
                 7.25 
                 c 
                 6.04 
                 cd 
               
               
                   
                 9363-16 
                 6.75 
                 c 
                 5.74 
                 cd 
               
               
                   
                 9449-27 
                 0.25 
                 c 
                 0.22 
                 d 
               
               
                   
                 9317-06 
                 0.00 
                 c 
                 0 
                 d 
               
               
                   
                 8909-05 
                 0.00 
                 c 
                 0 
                 d 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
             
           
               
                 TABLE 5 
               
               
                   
               
               
                 The average rate of population increase of phylloxera on nodosities 
               
               
                 formed on young root tips of advanced rootstock selections and a set 
               
               
                 of standard rootstocks. The phylloxera were selected from a vineyard 
               
               
                 planted on ‘101-14’ Mgt rootstock. 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 8909-05 
                 0.59 
               
               
                   
                 9363-16 
                 0.89 
               
               
                   
                 9365-43 
                 1.86 
               
               
                   
                 9365-85 
                 1.99 
               
               
                   
                 9407-14 
                 9.83 
               
               
                   
                 9449-27 
                 0.30 
               
               
                   
                 1103P 
                 2.10 
               
               
                   
                 101-14 Mgt 
                 7.98 
               
               
                   
                 Teleki 5C  
                 1.38 
               
               
                   
                 AxR#1 
                 2.15 
               
               
                   
                 O39-16 
                 0.43 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Number of root-knot nematode egg masses recovered from rootstock 
               
               
                 selections growing in 1 gal pots with soils collected from a vineyard 
               
               
                 site. This vineyard is known to have severe and chronic nematode 
               
               
                 pressure. Results per soils are means of three replicate pots. 
               
             
          
           
               
                   
                 Selection  
                 Soil 1 
                 Soil 2 
                 Soil 3 
               
               
                   
               
             
          
           
               
                   
                 Colombard  
                 69.02 
                 89.8 
                 74.6 
               
               
                   
                 Harmony 
                 31.2 
                 0 
                 2.8 
               
               
                   
                 St. George  
                 9.8 
                 26.2 
                 16.0 
               
               
                   
                 9365-85 
                 1 
                 0 
                 1.4 
               
               
                   
                 9407-14 
                 0 
                 0 
                 0 
               
               
                   
                 9363-16 
                 0 
                 0 
                 1.4 
               
               
                   
                 9365-43 
                 0 
                 0 
                 0.2 
               
               
                   
                 9449-27 
                 0 
                 0 
                 0.8 
               
               
                   
                 8909-05 
                 0 
                 0 
                 0

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