Patent Document

FIELD OF THE INVENTION 
       [0001]    The present invention is related to stable liquid pharmaceutical compositions of curcumin or its pharmaceutically acceptable salts or its derivatives. 
       BACKGROUND OF THE INVENTION 
       [0002]    Curcumin, a potent natural anti-oxidant and anti-inflammatory agent, has shown therapeutic potential against many diseases. These include cancers (colon, prostate, breast, skin, leukemia, etc.), atherosclerosis, stroke, CNS alcohol toxicity, traumatic brain injury, Huntington&#39;s disease, Marie-Charcot Tooth, multiple sclerosis and Alzheimer&#39;s disease. 
         [0003]    Curcumin in this specification is referred as total curcuminoids containing curcumin, desmethoxycurcumin and bis-desmethoxycurcumin. 
         [0004]    Curcumin is a crystalline compound and poorly soluble in water at pH less than 7. Major reasons contributing to the lower plasma and tissue levels of curcumin are due to poor absorption, high first pass metabolism and rapid systemic elimination. Most of the curcumin is not absorbed and simply passes through the GI tract and is excreted. Garcea, G. et al. (2004) reported that patients taking 3.6 g of curcumin a day (as a standard powder supplied by Sabinsa Corporation), drug levels in blood and liver were found to be negligible. 
         [0005]    Curcumin is not soluble at acidic pH and breaks down in solution at neutral or alkaline pH (e.g., in the GI tract, after the small intestine), In addition, curcumin is susceptible to rapid glucuronidation/sulfation. 
         [0006]    WO2007103435 discloses the compositions comprising curcuminoid, antioxidant (to stabilize curcumin against hydrolysis), water-soluble pharmaceutically acceptable carrier and optionally glucuronidation inhibitor. The said formulation with 35% w/w curcumin resulted in improved bioavailability. 
         [0007]    CN100352430C discloses self-micro-emulsified curcumin preparation comprising curcumin, surfactant, co-surfactant, oil phase and solid adsorbent providing self-micro-emulsified system having liquid drops with size below 100 nm. Such compositions show increase in curcumin solubility and absorption in gastrointestinal tract with higher bioavailability. The concentration of curcumin in composition is in the range of 0.33 to 1.56% w/v. 
         [0008]    WO2010010431 discloses self nano-emulsion compositions comprising curcumin (0.1 to 10% w/w), a lipidic carrier system with a hydrophilic-lipophilic balance (HLB) between 3 to 14 and a pH buffer. The lipidic carrier system is selected from one or more mono- and di- esters of saturated or unsaturated long chain fatty acids with low and medium molecular weight polyoxyethylene glycerol ethers or combinations thereof. The composition further comprises molecular aggregation inhibitor(s), a surface active agent and a co-solvent. The group of excipients used as lipidic carrier system belongs to alkyl-glycerol ether surfactants category (M. J. Lawrence et. al. Chemistry and Physics of Lipids, Volume 82, Issue 2, 19 August 1996, Pages 89-100) that led to increase in overall surfactant concentration up to 90% of the formulation. The higher concentration of surfactants in the formulation is undesirable as they are likely to cause gastric irritation and gastric mucosal damage. 
         [0009]    There is an unmet need to develop stable liquid pharmaceutical composition of curcumin or its pharmaceutically acceptable salts or its derivatives with higher curcumin concentration, without using acid buffer and/or molecular aggregation inhibitor(s) and without using high concentration of surfactants. 
       SUMMARY OF INVENTION 
       [0010]    The main object of the invention is to provide stable liquid pharmaceutical compositions of curcumin or its pharmaceutically acceptable salts or its derivatives with higher curcumin concentration and improved bioavailability. 
         [0011]    Another object of the invention is to provide stable pharmaceutical compositions wherein the concentration of curcumin in solution is 2 to 20% w/w. 
         [0012]    Another object of the invention is to provide the said pharmaceutical compositions without the use of pH buffer and/or molecular aggregation inhibitor(s). 
         [0013]    Further object of the invention is to provide a process for the preparation of the said pharmaceutical compositions. 
     
    
     
       DETAILED DESCRIPTION OF THE DRAWING 
         [0014]      FIG. 1  represents comparative bioavailability study of different pharmaceutical compositions of curcumin in rats (n=6). 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0015]    In accordance with this invention the stable liquid pharmaceutical compositions with higher curcumin concentration comprises of curcumin or pharmaceutically acceptable salts or derivatives thereof, surfactant, solvent, oil and optionally antioxidant wherein the concentration of surfactant is not more than 60% w/w. 
         [0016]    The compositions comprise 2 to 20% w/w of curcumin, oil, solvent, surfactant and optionally antioxidant, wherein the ratio of oil to solvent, surfactant to solvent and surfactant to curcumin is in the range of 0.83 to 10, 1 to 60 and 3 to 15, respectively. 
         [0017]    The said composition is prepared without the use of pH buffer and/or molecular aggregation inhibitor(s). 
         [0018]    The oil is selected from: (1) fractionated coconut oil, caprylic/capric triglyceride or oil containing fatty acid triglycerides, preferably medium chain fatty acid triglycerides (2) isopropyl myristate, isopropyl palmitate, ethyl linoleate or oil containing ethyl oleate esters of fatty acids and monovalent alkanols (3) propyleneglycol dicaprylate, propyleneglycol dilaurate or oil containing propyleneglycol di-fatty acid esters. 
         [0019]    The surfactant is selected from polysorbate, vitamin E TPGS and cremophor, preferably cremophor. 
         [0020]    The solvent is selected from glycofurol, polyethylene glycol (PEG 200, 400), glycerol, polypropylene glycol, propylene glycol, N-methyl-2-pyrolidone and ethyl alcohol or mixture thereof, preferably from propylene glycol and ethyl alcohol or mixture thereof. 
         [0021]    The anti-oxidant is selected from ascorbyl palmitate, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, sodium ascorbate, tocopheryl derivative such as alpha-tocopherol and mixtures thereof. 
         [0022]    The pharmaceutical composition as per the invention shows improved bioavailability. 
         [0023]    The present invention provides a process for the preparation of the said composition comprising
       (i) dispersing curcumin in solvent;   (ii) adding surfactant to the curcumin dispersion of step (i);   (iii) dissolving curcumin by mixing and heating; and   (iv) adding oil to the solution of step (iii) to obtain the liquid pharmaceutical composition.       
 
         [0028]    In one embodiment a process for the preparation of the said composition comprising:
       (i) dispersing curcumin in surfactant;   (ii) adding solvent to the curcumin dispersion of step (i); and dissolving curcumin by mixing and heating; and   (iii) mixing oil to the solution of step (ii) to obtain the stable liquid pharmaceutical composition.       
 
         [0032]    In another embodiment a process for the preparation of the said composition comprising:
       (i) dispersing curcumin in oil;   (ii) adding solvent to the curcumin dispersion of step (i); and   (iii) mixing surfactant to the dispersion of step (ii) and dissolving curcumin by mixing and heating to obtain the stable liquid pharmaceutical composition.       
 
         [0036]    In another embodiment a process for the preparation of the said composition comprising:
       (i) mixing solvent, surfactant and oil;   (ii) adding curcumin to blend of step (i) and dissolving curcumin by mixing and heating to obtain stable liquid pharmaceutical composition.       
 
         [0039]    The compositions according to the present invention can be administered by oral, topical or parenteral route; preferably the compositions are administered by oral route. 
         [0040]    The invention is further illustrated with following non-limiting examples. 
       EXAMPLE 1 
       [0041]    A pharmaceutical composition of curcumin was prepared as given in table 1. 
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Composition for example 1 
               
             
          
           
               
                 S. No. 
                 Ingredients 
                 Qty (% w/w) 
               
               
                   
               
             
          
           
               
                 1. 
                 Curcumin 
                 5.00 
               
               
                 2. 
                 Cremophor RH 40 
                 47.00 
               
               
                   
                 (Polyoxyl Hydrogenated Castor Oil) 
               
               
                 3. 
                 Crodamol GTCC 
                 25.00 
               
               
                   
                 (Caprylic/Capric Triglycerides) 
               
               
                 4. 
                 Ethyl Alcohol 
                 9.20 
               
               
                 5. 
                 Alpha-Tocopherol 
                 0.47 
               
               
                 6. 
                 Propylene Glycol 
                 13.33 
               
               
                   
               
               
                 1. Curcumin was dispersed in ethyl alcohol and propylene glycol. 
               
               
                 2. Cremophore RH 40 was added to curcumin dispersion of step 1 with mixing and heating to dissolve the curcumin. 
               
               
                 3. Crodamol GTCC, and alpha-tocopherol were added to the mixture of step 2 and mixed. 
               
             
          
         
       
     
         [0042]    The formulation after dilution with water (1:1000) or 0.1 N HCl (1:1000) resulted in clear and stable solution. Particle size of resulting micro-emulsion was found below 100 nm after dilution of 24 Hr. 
       EXAMPLE 2 
       [0043]    A pharmaceutical composition of curcumin was prepared as given in table 2. 
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Composition for example 2 
               
             
          
           
               
                 S. No. 
                 Ingredients 
                 Qty (% w/w) 
               
               
                   
               
             
          
           
               
                 1. 
                 Curcumin 
                 21.00 
               
               
                 2. 
                 Cremophor RH 40 
                 35.00 
               
               
                   
                 (Polyoxyl hydrogenated castor 
               
               
                   
                 oil) 
               
               
                 3. 
                 Crodamol GTCC 
                 17.50 
               
               
                   
                 (Caprylic/Capric 
               
               
                   
                 Triglycerides) 
               
               
                 4. 
                 Ethyl Alcohol 
                 15.50 
               
               
                 5. 
                 Propylene Glycol 
                 11 
               
               
                   
               
               
                 1. Curcumin was dispersed in ethyl alcohol. 
               
               
                 2. Cremophore RH 40 was added to the curcumin dispersion of step 1 with mixing and heating to dissolve the curcumin. 
               
               
                 3. Crodamol GTCC and propylene glycol were added to the mixture of step 2 and mixed. 
               
             
          
         
       
     
         [0044]    Particle size of the resulting microemulsion was found to be 222 nm. 
       EXAMPLES 3-8 
       [0045]    Pharmaceutical compositions of curcumin were prepared as given in table 3. 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
               
               
               
             
               
               
               
             
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Composition for examples 3-8 
               
             
          
           
               
                   
                 Example No. 
               
             
          
           
               
                 S. 
                 Ingre- 
                 3 
                 4 
                 5 
                 6 
                 7 
                 8 
               
             
          
           
               
                 No. 
                 dients 
                 Qty (% w/w) 
               
               
                   
               
             
          
           
               
                 1 
                 Curcumin 
                 3 
                 5 
                 3 
                 2 
                 6 
                 7.5 
               
               
                 2 
                 Cremophor 
                 30 
                 29.40 
                 59.0 
                 39.2 
                 46.5 
                 45.8 
               
               
                   
                 RH 40 
               
               
                 3 
                 Cradamol 
                 4.71 
                 4.60 
                 37.6 
                 50 
                 24.7 
                 24.3 
               
               
                   
                 GTCC 
               
               
                 4 
                 Ethanol 
                 20 
                 19.60 
                 0.5 
                 7 
                 9.1 
                 9.0 
               
               
                 5 
                 Vit E. 
                 — 
                 — 
                 — 
                 — 
                 0.5 
                 0.5 
               
               
                   
                 Acetate 
               
               
                 6 
                 Propylene 
                 42.29 
                 41.40 
                 — 
                 1.8 
                 13.2 
                 13.0 
               
               
                   
                 Glycol 
               
               
                 7 
                 Obser- 
                 Clear 
                 Clear 
                 Clear 
                 Clear 
                 Clear 
                 Clear 
               
               
                   
                 vation 
                 solu- 
                 solu- 
                 solu- 
                 solu- 
                 solu- 
                 solu- 
               
               
                   
                   
                 tion 
                 tion 
                 tion 
                 tion 
                 tion 
                 tion 
               
               
                 8 
                 Particle 
                 108.5 
                 1053 
                 85.12 
                 169.5 
                 43.32 
                 737.8 
               
               
                   
                 size (nm) 
               
               
                   
               
               
                 1. Curcumin was dispersed in ethyl alcohol. 
               
               
                 2. Cremophore RH 40 was added to the curcumin dispersion of step 1 with mixing and heating. 
               
               
                 3. Crodamol GTCC, propylene glycol were added to the mixture of step 2 and mixed. 
               
             
          
         
       
     
       EXAMPLE 9 
       [0046]    Pharmaceutical composition from examples 1 was further subjected to bioavailability study in rat (n=6) at dose of 150 mg/kg body weight as given in table 4. 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Different formulation subjected to animal study 
               
             
          
           
               
                   
                 Groups 
                 Pharmaceutical Composition 
               
               
                   
                   
               
               
                   
                 Control 
                 drug dispersed in 2% sodium 
               
               
                   
                   
                 CMC 
               
               
                   
                 Test 
                 As per example 1 
               
               
                   
                   
               
             
          
         
       
     
         [0047]    Test demonstrated enhanced bioavailability as compared to control ( FIG. 1 ). 
       EXAMPLE 10 
       [0048]    Pharmaceutical composition of example 1 was subjected to accelerated stability study as per ICH guideline. The product was found to be stable for 6 months at 40° C./75% RH as shown in table 5. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Stability data at 40° C./75% RH 
               
             
          
           
               
                 Parameters 
                 Initial 
                 1 M 
                 2 M 
                 3 M 
                 6 M 
               
               
                   
               
               
                 Assay 
                 97.1% 
                 92.0% 
                 89.2% 
                 90.1% 
                 94.8% 
               
               
                 pH (10% 
                 6.72 
                 6.4 
                 6.15 
                 6.4 
                 6.15 
               
               
                 aqueous 
               
               
                 solution) 
               
               
                 Globule 
                 69.92 nm 
                 41.90 nm 
                 44.00 nm 
                 25.05 nm 
                 50.88 nm 
               
               
                 size (10% 
               
               
                 aqueous 
               
               
                 solution) 
               
               
                   
               
             
          
         
       
     
       EXAMPLE 11 
       [0049]    Pharmaceutical composition of example 1 and marketed formulations (Curcugel and Curcugel Ultra) were subjected to in vivo pharmacokinetic study in human volunteers (n=3 for each group). 
         [0050]    The values of peak plasma concentration level (C max ) and area under the curve (AUC) obtained after administration of the pharmaceutical composition are shown in table 6. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Pharmacokinetic parameters calculated from the plasma profile 
               
               
                 obtained after administration of 400 mg of pharmaceutical 
               
               
                 composition of example 1 and Curcugel 250 mg and Curcugel 
               
               
                 Ultra 500 mg in human volunteers (n = 3) each. 
               
             
          
           
               
                 Pharmacokinetic 
                 Composition as per present 
                   
                 Curcugel  
               
               
                 parameters 
                 invention 
                 Curcugel 
                 Ultra 
               
               
                   
               
             
          
           
               
                 C max  (ng/ml) 
                 33.05 
                 Non 
                 Non 
               
               
                   
                   
                 detectable 
                 detectable 
               
               
                 T max  (h) 
                 0.58 
                 — 
                 — 
               
               
                 AUC 0-24 h   
                 24.395 
                 — 
                 — 
               
               
                 (ng/ml)h 
               
               
                 AUC 0-∞ h   
                 25.615 
                 — 
                 — 
               
               
                 (ng/ml)h 
               
               
                   
               
             
          
         
       
     
         [0051]    The pharmaceutical composition comprising curcumin, surfactant, solvent, oil and anti-oxidant was found to exhibit high bioavailability as shown in table 6.

Technology Category: 1