[RESULTS] There was significant difference in duration of invasive mechanical ventilation [ 7.0 ( 6.8 , 9.5 ) days vs. 21.0 ( 17.0 , 25.0 ) days , Z = -6.048 , P = 0.000 ] , duration of total mechanical ventilation ( 18.04.1 days vs. 22.07.3 days , t = -2.805 , P = 0.008 ) , and length of ICU stay ( 21.04.1 days vs. 28.08.1 days , t = -4.012 , P = 0.000 ) between sequential group and control group , but there was no significant differences in the incidence of VAP [ 15.4 % ( 4/26 ) vs. 29.6 ( 8/27 ) , ( 2 ) = 1.535 , P = 0.215 ] and mortality rate [ 7.7 % ( 2/26 ) vs. 18.5 % ( 5/27 ) , P = 0.420 ] .