Source: http://www.google.com/patents/US7586600?dq=5,072,412
Timestamp: 2015-09-04 02:38:21
Document Index: 621257790

Matched Legal Cases: ['Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60']

Patent US7586600 - Normalization of data - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsMethods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to...http://www.google.com/patents/US7586600?utm_source=gb-gplus-sharePatent US7586600 - Normalization of dataAdvanced Patent SearchPublication numberUS7586600 B2Publication typeGrantApplication numberUS 12/176,656Publication dateSep 8, 2009Filing dateJul 21, 2008Priority dateSep 19, 2003Fee statusPaidAlso published asUS7417726, US8089623, US20060024831, US20080288179, US20100220321Publication number12176656, 176656, US 7586600 B2, US 7586600B2, US-B2-7586600, US7586600 B2, US7586600B2InventorsH. Pin Kao, Ian A. Harding, Achim Karger, Mark F. Oldham, Omead Ostadan, Greg YoungOriginal AssigneeApplied Biosystem, LlcExport CitationBiBTeX, EndNote, RefManPatent Citations (1), Referenced by (4), Classifications (54), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetNormalization of data
US 7586600 B2Abstract
Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
Images(137) Claims(17)
1. A method of normalizing spectral sensitivity from an instrument, the instrument using a microplate having a plurality of reaction chambers, the instrument having a plurality of filters, the method comprising:
depositing a first reference standard into a first of the plurality of reaction chambers in the microplate, the first reference standard outputting a first reference standard signal;
depositing a sample aliquot and at least one dye into a second of the plurality of reaction chambers, the sample aliquot having at least a portion of a polynucleotide sample, the dye outputting a data signal representative of the polynucleotide sample;
subjecting the sample aliquot and the first reference standard to a testing regimen, wherein the polynucleotide sample in the sample aliquot is amplified, the first reference standard signal is emitted from the first of the plurality of reaction chambers containing the first reference standard, and the data signal is emitted from the second of the plurality of reaction chambers;
collecting and analyzing the first reference standard signal relative to a predetermined level to generate a normalization bias; and
normalizing the data signal relative to the normalization bias.
depositing a second reference standard into a second of the plurality of reaction chambers in the microplate, the second reference standard outputting a second reference standard signal.
3. The method according to claim 2 wherein the collecting and analyzing the first reference standard signal relative to a predetermined level to generate a normalization bias comprises collecting and analyzing the first and second reference standard signals relative to a predetermined level to generate a normalization bias.
4. The method according to claim 1 wherein the at least one dye comprises a series dilution.
5. The method according to claim 1 wherein the at least one dye comprises multiple titration points.
6. The method according to claim 1 wherein the at least one dye comprises dye ladders.
7. The method according to claim 1 further comprises performing reverse transcription on the sample aliquot.
8. A method of normalizing data output from an instrument, the instrument using a microplate having a plurality of reaction chambers, the method comprising:
providing a first of the plurality of reaction chambers in the microplate, the first of the plurality of reaction chambers being free from a fluorescing substance;
depositing a sample aliquot and at least one dye into a second of the plurality of reaction chambers, the sample aliquot having at least a portion of a polynucleotide sample, the dye outputting a data signal in response to the polynucleotide sample;
collecting and analyzing a signal from the first of the plurality of reaction chambers to determine a level of background correction to generate a normalization bias; and
depositing a non-template control in the first of the plurality of reaction chambers.
10. The method according to claim 8 wherein the at least one dye comprises a series dilution.
11. The method according to claim 8 wherein the at least one dye comprises multiple titration points.
12. The method according to claim 8 wherein the at least one dye comprises dye ladders.
13. A method of normalizing data output from an instrument, the instrument using a microplate having a plurality of reaction chambers, the method comprising:
depositing a first control into a first of the plurality of reaction chambers in the microplate, the first control outputting a first control signal, the first of the plurality of reaction chambers being free of a polynucleotide sample;
collecting and analyzing the first control signal relative to a predetermined level to generate a normalization bias; and
15. The method according to claim 13 wherein the at least one dye comprises a series dilution.
16. The method according to claim 13 wherein the at least one dye comprises multiple titration points.
17. The method according to claim 13 wherein the at least one dye comprises dye ladders.
This application is a continuation of U.S. patent application Ser. No. 11/087,103 filed on Mar. 22, 2005, which is a continuation-in-part of U.S. patent application Ser. No. 10/913,601 filed on Aug. 5, 2004, U.S. patent application Ser. No. 10/944,673 filed on Sep. 17, 2004, now abandoned, and U.S. patent application Ser. No. 10/944,691 filed on Sep. 17, 2004, now abandoned. U.S. patent application Ser. No. 10/944,673 claims a benefit to U.S. Provisional Application No. 60/504,500 filed on Sep. 19, 2003; U.S. Provisional Application No. 60/504,052 filed on Sep. 19, 2003; U.S. Provisional Application No. 60/589,224 filed Jul. 19, 2004; U.S. Provisional Application No. 60/589,225 filed on Jul. 19, 2004; and U.S. Provisional Application No. 60/601,716 filed on Aug. 13, 2004. U.S. patent application Ser. No. 10/944,691 is a continuation-in-part of U.S. patent application Ser. No. 10/913,601 filed on Aug. 5, 2004, which further claims the benefit of U.S. Provisional Application No. 60/504,052 filed on Sep. 19, 2003; U.S. Provisional Application No. 60/504,500 filed on Sep. 19, 2003; U.S. Provisional Application No. 60/589,224 filed Jul. 19, 2004; U.S. Provisional Application No. 60/589,225 filed on Jul. 19, 2004; and U.S. Provisional Application No. 60/601,716 filed on Aug. 13, 2004.
FIG. 94 is a perspective view illustrating a source plate and wash station,
wherein the source plate stays lidded and the washing tray can be accessed by a dispensing device according to some embodiments;
The following description of some embodiments is merely exemplary in nature and is in no way intended to limit the present teachings, applications, or uses. Although the present teachings will be discussed in some embodiments as relating to polynucleotide amplification, such as PCR, such discussion should not be regarded as limiting the present teaching to only such applications.
In some embodiments, the surface of the microplate 20 comprises an enhanced surface which can comprise a physical or chemical modality on or in the surface of the microplate so as to enhance support of, or filling of, assay 1000 in a material retention region (e.g., a well or a reaction spot). Such modifications can include chemical treatment of the surface, or coating the surface. In some embodiments, such ch