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Patent US5506146 - Measurement of the activated partial thromboplastin time (APTT) in a one ... - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsA method for the determination of the activated partial thromboplastin time (APTT) in a one-step reaction, in which the determination of APTT is initiated by contacting a reagent that contains the substances necessary for the APTT determination in a premixed form, with the sample to be tested. A reagent...http://www.google.com/patents/US5506146?utm_source=gb-gplus-sharePatent US5506146 - Measurement of the activated partial thromboplastin time (APTT) in a one-step reactionAdvanced Patent SearchTry the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.Publication numberUS5506146 APublication typeGrantApplication numberUS 08/266,704Publication dateApr 9, 1996Filing dateJun 28, 1994Priority dateJun 30, 1993Fee statusPaidAlso published asDE59309435D1, EP0633473A1, EP0633473B1Publication number08266704, 266704, US 5506146 A, US 5506146A, US-A-5506146, US5506146 A, US5506146AInventorsDieter JosefOriginal AssigneeStiftung Fur Diagnostische ForschungExport CitationBiBTeX, EndNote, RefManPatent Citations (14), Non-Patent Citations (7), Referenced by (14), Classifications (12), Legal Events (5) External Links: USPTO, USPTO Assignment, EspacenetMeasurement of the activated partial thromboplastin time (APTT) in a one-step reaction
In addition it is preferred that the reagent is stabilized by one or several amino acids. The shelf life of the reagent even at higher temperatures (e.g., 37° C.) is considerably increased by this stabilization. Amino acids that are selected from the group comprising D-alanine, L-alanine, β-alanine, glycine and valine are preferably used for the stabilization. Moreover it is preferred that the amino acids are present at a total concentration of 0.5 to 10% in relation to the total weight of the reagent.
The experiment according to example 1 is repeated with the addition of amounts of amino acids as stated in each case in order to improve the stability. The degree of stabilization at various concentrations is determined with normal plasma in a stress test by storing the reagent at 37° C. The results are summarized in Table 2. According to this the reagent is very well stabilized by D-alanine, L-alanine, β-alanine and glycine.
TABLE 2a______________________________________Clotting time in sec.D-alanine   start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      80     116        129      1400.625%  75     96         102      1171.25%   73     88         90       992.5%    72     81         82       855.0%    73     75         74       74______________________________________
TABLE 2b______________________________________Clotting time in sec.L-alanine   start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      80     107        135      1450.625%  75     91         103      1191.25%   74     86         92       1022.5%    75     80         83        865.0%    77     79         80        80______________________________________
TABLE 2c______________________________________Clotting time in sec.&#946;-alanine   start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      81     108        130      1440.625%  76     96         107      1181.25%   74     88         93       1002.5%    75     74         82        835.0%    77     79         79        78______________________________________
TABLE 2d______________________________________Clotting time in sec.glycine start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      81     110        136      1360.625%  76     92         98       1031.25%   75     84         90       922.5%    75     79         82       805.0%    83     85         87       87______________________________________
TABLE 2e______________________________________Clotting time in sec.valine  start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      86     111        130      1450.625%  80     98         106      1261.25%   79     91         95       1052.5%    78     88         92        965.0%    82     91         94        95______________________________________
TABLE 2f______________________________________Clotting time in sec.lysine start    3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%    78       108        131      1400.625% 80        91        104      1281.25% 93       106        120      1182.5%  160      202        --       --5.0%  &gt;250     --         --       --______________________________________
TABLE 2g______________________________________Clotting time in sec.methionine   start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      81     108        125      1400.625%  75     94         99       1191.25%   76     90         95       1102.5%    81     95         96       1105.0%    105    122        --       --______________________________________
TABLE 2h______________________________________glutamic   Clotting time in sec.acid    start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      82     110        131      1420.625%  74     93         102      1201.25%   72     86          91      1022.5%    78     87          92      1035.0%    122    160        168      180______________________________________
TABLE 2i______________________________________Clotting time in sec.N-acetyl-                             2 weekscysteine   start    3 days 37° C.                       1 week 37° C.                                 37° C.______________________________________0%       83      117        137       1420.625%  122      166        176       &gt;2501.25%   228      &gt;250       --        --2.5%    &gt;250     --         --        --5.0%    &gt;250     --         --        --______________________________________
TABLE 2j______________________________________aspartic   Clotting time in sec.acid    start  3 days 37° C.                     1 week 37° C.                              2 weeks 37° C.______________________________________0%      81     109        135      1360.625%  74     88         102      1091.25%   73     83          90       922.5%    77     82          89       885.0%    110    118        140      130______________________________________
TABLE 2k______________________________________Clotting time in sec.                                 2 weeksarginine   start    3 days 37° C.                       1 week 37° C.                                 37° C.______________________________________0%       83      117        132       1400.625%   83       85        125       1411.25%   103      165        160       1652.5%    214      &gt;250       --        --5.0%    &gt;250     --         --        --______________________________________
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Chem., One Step Chromogenic Equivalent of Activated Partial Thromboplastin Time Evaluated for Clinical Application , 37/7, 1235 1244 (1991).* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS5780255 *Jun 9, 1995Jul 14, 1998Instrumentation Laboratory, S.P.A.Protein C pathway screening testUS6451610 *Apr 14, 1999Sep 17, 2002International Technidyne CorporationMethod and apparatus for coagulation based assaysUS6632678Jan 3, 2001Oct 14, 2003Sienco, Inc.Method for performing activated clotting time test with reduced sensitivity to the presence of aprotinin and for assessing aprotinin sensitivityUS6898532 *Feb 28, 2003May 24, 2005Biomerieux, Inc.Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sampleUS7179612Jun 8, 2001Feb 20, 2007Biomerieux, Inc.Method for detecting a lipoprotein-acute phase protein complex and predicting an increased risk of system failure or mortalityUS7211438Jul 2, 2004May 1, 2007Biomerieux, Inc.Method and apparatus for predicting the presence of haemostatic dysfunction in a patient sampleUS8357539 *Dec 22, 2010Jan 22, 2013Sysmex CorporationActivated partial thromboplastin time measuring reagent, activated partial thromboplastin time measuring method, and determination method for determining presence or absence of blood coagulation inhibitorUS20030049851 *May 28, 2002Mar 13, 2003Toh Cheng HockMethod for predicting the presence of haemostatic dysfunction in a patient sampleUS20040248308 *Jul 2, 2004Dec 9, 2004Toh Cheng HockMethod and apparatus for predicting the presence of haemostatic dysfunction in a patient sampleUS20110159597 *Dec 22, 2010Jun 30, 2011Sysmex CorporationActivated partial thromboplastin time measuring reagent, activated partial thromboplastin time measuring method, and determination method for determining presence or absence of blood coagulation inhibitorCN101221189BJan 12, 2007Aug 15, 2012上海太阳生物技术有限公司External diagnostic reagent kit used for measuring activated partial thromboplastin timeCN101226201BFeb 5, 2008May 11, 2011长安大学Agent for activation sector cruor activating-enzyme time (APTT)CN105445478A *Nov 12, 2015Mar 30, 2016武汉中太生物技术有限公司Activated partial thromboplastin time measurement kit and preparation method thereofWO1998044352A1 *Mar 26, 1998Oct 8, 1998Baxter AktiengesellschaftREAGENT FOR DETERMINING aPTT* Cited by examinerClassifications U.S. Classification436/69, 435/13, 530/383, 436/34, 514/13.7International ClassificationC12Q1/56, G01N33/86Cooperative ClassificationG01N33/86, C12Q1/56, G01N2405/04European ClassificationG01N33/86, C12Q1/56Legal EventsDateCodeEventDescriptionJun 28, 1994ASAssignmentOwner name: STIFTUNG FUR DIAGNOSTISCHE FORSCHUNG, SWITZERLANDFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:JOSEF, DIETER;REEL/FRAME:007056/0461Effective date: 19940627Dec 3, 1996CCCertificate of correctionOct 8, 1999FPAYFee paymentYear of fee payment: 4Sep 30, 2003FPAYFee paymentYear of fee payment: 8Sep 25, 2007FPAYFee paymentYear of fee payment: 12RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services