Source: http://www.google.com/patents/US6071391?dq=4200770
Timestamp: 2017-07-27 05:46:08
Document Index: 696752750

Matched Legal Cases: ['art. 2', 'art.\n23', 'art.\n29', 'art.\n34', 'art 20', 'art 20', 'art 23', 'art 24', 'art 23']

Patent US6071391 - Enzyme electrode structure - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsA biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed...http://www.google.com/patents/US6071391?utm_source=gb-gplus-sharePatent US6071391 - Enzyme electrode structureAdvanced Patent SearchTry the new Google Patents, with machine-classified Google Scholar results, and Japanese and South Korean patents.Publication numberUS6071391 APublication typeGrantApplication numberUS 08/990,997Publication dateJun 6, 2000Filing dateDec 15, 1997Priority dateSep 12, 1997Fee statusPaidAlso published asUS6156173, US6503381, US6893545, US7713406, US7901554, US7905998, US7918988, US7998336, US8414761, US8557103, US20030102213, US20050258052, US20080251377, US20080251378, US20080257725, US20080257728, US20110308970, US20120055812Publication number08990997, 990997, US 6071391 A, US 6071391A, US-A-6071391, US6071391 A, US6071391AInventorsMasao Gotoh, Hiroki Mure, Hiroshi ShirakawaOriginal AssigneeNok CorporationExport CitationBiBTeX, EndNote, RefManPatent Citations (28), Referenced by (694), Classifications (15), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetEnzyme electrode structure
US 6071391 AAbstract
1. A biosensor comprising an upper plate, a lower plate, a space formed between the two plates for sucking and housing a sample therein, a layer for fixing the two plates together, the space for sucking and housing the sample being partially opened at a peripheral part thereof and partially closed by the layer, a working electrode provided on at least one of the two plates and having at least glucose oxidase and a counter electrode fixed relative to the working electrode, each of the two plates lacking an air vent or hole for connecting to the space,one end of each of the two plates having a tapered part, and a tip part of the working electrode or a tip part of the counter electrode provided on the tapered part. 2. A biosensor according to claim 1, wherein the working electrode and the counter electrode are formed on the upper and lower plates, respectively, so as to face each other.
3. A biosensor according to claim 1, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.
4. A biosensor according to claim 1, wherein the glucose oxidase is fixed by applying and drying.
5. A biosensor according to claim 1, wherein the layer is an adhesive layer formed by a double-sided adhesive tape.
6. A biosensor according to claim 1, wherein the layer is an adhesive layer or a spacer with adhesive on both sides thereof which is interposed between the two plates.
7. A biosensor according to claim 1, wherein a reference electrode is arranged at a side of the working electrode or the counter electrode.
8. A biosensor according to claim 1, wherein the layer is an adhesive layer formed by applying an adhesive to one of the two plates.
9. A biosensor according to claim 1, wherein the layer is an adhesive layer formed by applying an adhesive to both the plates.
10. A biosensor according to claim 1, wherein an insulating layer is formed between one of the two plates and the layer.
11. A biosensor according to claim 1, wherein one end of each of the plates is formed into a protruding shape.
12. A biosensor according to claim 1, wherein an oxidoreductase is fixed on the counter electrode.
13. A biosensor according to claim 1, wherein the space is closed by a closing edge of the layer which extends between two opposite side edges of the two plates, and the space is open at a peripheral portion of the two plates outside of the edge of the layer where the layer is not provided.
14. A biosensor according to claim 13, wherein the closing edge of the layer is substantially straight.
15. A biosensor which comprises a lower base, an upper base, a working electrode, an oxidoreductase fixed on the working electrode, and a counter electrode, wherein the working electrode and the counter electrode are fixed on the lower base and the upper base through an adhesive layer so as to have a facing structure, and a lead part for each of the two electrodes is formed in a position of the lower base where an end part thereof is never superposed on the upper base, the electrode on the upper base being conducted to its lead part through the adhesive layer.
16. A biosensor according to claim 15, wherein the conductivity through the adhesive layer is performed by a conductive material provided in a bored part formed in a double-sided adhesive tape.
17. A biosensor according to claim 15, wherein the conductivity through the adhesive layer is performed by a conductive material provided in a bored part formed in the adhesive layer.
18. A biosensor according to claim 15, wherein the conductivity through the adhesive layer is performed by a conductive material filled in bored parts formed in the adhesive layer provided on each of the bases.
19. A biosensor according to claim 15, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.
20. A biosensor according to claim 15, wherein oxidoreductase-electron transmitter mixture layers are provided on the working electrode and the counter electrode.
21. A biosensor according to claim 15, further comprising a reference electrode fixed to one of the bases.
22. A biosensor according to claim 15, wherein one end of each of the bases has a tapered part, and a tip part of the working electrode or a tip part of the counter electrode is provided on each tapered part.
23. A biosensor according to claim 15, wherein the layer has a substantially straight edge for closing an inner edge of a space for sucking and housing a sample.
24. A biosensor comprising a longer upper base, a shorter lower base, a working electrode having an oxidoreductase fixed on the working electrode and a counter electrode, wherein the working electrode and the counter electrode are fixed on the longer upper base and the shorter lower base through an adhesive layer so as to have a facing structure, and lead parts for these electrodes are formed on the upper base, wherein the electrode on the upper base is conducted to its lead part through the upper base, and the electrode on the lower base is conducted to its lead part provided on the upper base through the adhesive layer.
25. A biosensor according to claim 24, wherein the layer has a substantially straight edge for closing an inner edge of a space for sucking and housing a sample.
26. A biosensor which comprises a shorter upper base, a longer lower base, a working electrode, an oxidoreductase fixed on the working electrode, and a counter electrode, wherein the working electrode and the counter electrode are fixed on the longer lower base and the shorter upper base through a spacer so as to have a facing structure, and a lead part for each of the two electrodes is formed in a position on the lower base, wherein the electrode on the upper base is conducted to its lead part through the spacer.
27. A biosensor according to claim 26, wherein the conductivity through the spacer is performed by a conductive material provided in a bored part formed in the spacer.
28. A biosensor according to claim 26, wherein one end of each of the two bases has a tapered part, and a tip part of the working electrode or a tip part of the counter electrode is provided on each tapered part.
29. A biosensor according to claim 26, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.
30. A biosensor according to claim 26, wherein oxidoreductase-electron transmitter mixture layers are provided on the working electrode and the counter electrode.
31. A biosensor according to claim 26, further comprising a reference electrode fixed to at least one of the two bases.
32. A biosensor according to claim 31, wherein the conductivity through the spacer is performed by a conductive material filled in a bored part formed in the spacer.
33. A biosensor according to claim 31, wherein one end of each of the two bases has a tapered part, and a tip part of the working electrode or a tip part of the counter electrode is provided on each tapered part.
34. A biosensor according to claim 31 wherein an oxidoreductase-electron transmitter mixture layer is formed on the working electrode.
35. A biosensor according to claim 31 wherein oxidoreductase-electron transmitter mixture layers are formed on the working electrode and the counter electrode.
36. A biosensor according to claim 26, wherein the layer has a substantially straight edge for closing an inner edge of a space for sucking and housing a sample.
37. A biosensor which comprises a lower base, an upper base, a working electrode, an oxidoreductase on the working electrode and a counter electrode, wherein the working electrode and the counter electrode are fixed on the upper base and the lower base through a spacer so as to have a facing structure, and two lead parts for the two electrodes formed on the upper base, wherein the electrode on the upper base is conducted to its lead part through the upper base, and the electrode on the lower base is conducted to its lead part on the upper base through the spacer.
38. A biosensor according to claim 37, wherein the conductivity through the base is performed by a conductive material provided in a bored part formed in the base.
39. A biosensor device which comprises a device body having a connector part, a working electrode and a counter electrode, the connector part having input terminals, a reaction sensor member which is to be inserted into the connector part of the device body in such a manner as to be attachable and detachable, the reaction sensor member having an output terminal of the working electrode and an output terminal of the counter electrode so as to be electrically connected to the input terminals of the connector part when the reaction sensor member is inserted into the connector part of the device body, and wherein a reaction part is formed on at least the working electrode, the reaction sensor member further having a sensor insertion judging electrode, the connector part of the device body having two input terminals, wherein the sensor insertion judging electrode has an output terminal which makes contact with the two input terminals of the connector part, so that the device body is started when the output terminal of the sensor insertion judging electrode becomes in contact with the two input terminals.
40. A biosensor device according to claim 39, further comprising a control part which judges sucking of a sample to be measured by a signal for laying the working electrode and the counter electrode into closed circuit by the sucking of the sample to the reaction part, starts to count residual time up to a preset measurement end, transmits a count signal to a display part provided on the device body and displays it.
41. A biosensor device according to claim 39, wherein the working electrode and the counter electrode are placed along a common plane in relation to the reaction sensor member.
42. A biosensor device according to claim 39, wherein the reaction part is formed as a layer made of an oxidoreductase and an electron transmitter in mixture.
43. A biosensor device according to claim 39 wherein a sample to be measured is sucked onto the reaction part in a state where a voltage is applied between the working electrode and the counter electrode after the sensor insertion, and a current value is generated by oxidation and then is taken out so as to be measured.
(A) Embodiments of FIGS. 1a to FIG. 5
Glucose+2Fe(CN)6  . . . +H2 O&#8594;
gluconic acid+2H+ +2Fe(CN)6  . . .
2Fe(CN)6 . . . &#8594;2Fe(CN)6. . . +2e- When parabenzoquinone is used instead of potassium ferricyanide as mediator, hydroquinone is generated by the reaction of glucose with parabenzoquinone under the presence of GOD, the generated hydroquinone is oxidized by the working electrode to generate oxidizing current, and its value is measured.
hydroquinone&#8594;parabenzoquinone+2H+ +2e- On the other hand, although the counter electrode is usable without particularly immobilizing anything thereon, a layer consisting of at least one of albumin and electron acceptor may be formed thereon. In this case, the inclination of concentration apt to be caused in dissolution or dispersion of the mixture layer by the sample solution, which is observed when the mixture layer is provided only on the working electrode, is advantageously eliminated, and the measuring precision is also improved.
TABLE______________________________________Glucose concentration (mg/dl)               A        B______________________________________0                   0.5 &#956; A                        0 &#956; A50                  1 &#956; A 1 &#956; A100                 2 &#956; A 2 &#956; A250                 5 &#956; A 4 &#956; A500                 9 &#956; A 8 &#956; A800                 14 &#956; A                        13 &#956; A1000                18 &#956; A                        16 &#956; A______________________________________
(1) The base A and the ba se B were stuck together by the adhesive layer provided on the base A side.
As the part for controlling the system, the device body 10 has a control part 20 formed of a CPU (central processing unit) by microcomputer The control part 20 is formed of a current-voltage converting circuit 21 for converting the detected current to voltage value, an amplifying circuit 22 for amplifying the converted voltage signal, an arithmetic part 23 for arithmetically processing on the basis of the input data signal, and a display part 24 such as LCD (liquid crystal display device) for digitally displaying the value processed in the arithmetic part 23 as measurement data. In the CPU, the entire control is performed on the basis of signals inputted and outputted through an I/O port from each part and each circuit.
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EventsDateCodeEventDescriptionApr 20, 1998ASAssignmentOwner name: NOK CORPORATION, JAPANFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:GOTOH, MASAO;MURE, HIROKI;SHIRAKAWA, HIROSHI;REEL/FRAME:009118/0297Effective date: 19980120Sep 12, 2002ASAssignmentOwner name: THERASENSE, INC., CALIFORNIAFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:NOK CORPORATION;REEL/FRAME:013280/0676Effective date: 20020904Dec 4, 2003FPAYFee paymentYear of fee payment: 4Oct 31, 2006ASAssignmentOwner name: ABBOTT DIABETES CARE INC., CALIFORNIAFree format text: CHANGE OF NAME;ASSIGNOR:THERASENSE, INC.;REEL/FRAME:018584/0249Effective date: 20050801Sep 14, 2007FPAYFee paymentYear of fee payment: 8Sep 23, 2011FPAYFee paymentYear of fee payment: 12RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services