Source: http://www.google.com/patents/US5371020?dq=6514640
Timestamp: 2015-04-25 22:53:11
Document Index: 203897826

Matched Legal Cases: ['art 410', 'art 412', 'art 410', 'art 410', 'art 412', 'art 410', 'art 412']

Patent US5371020 - Method of photometric in vitro determination of the content of an analyte in ... - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inAdvanced Patent SearchPatentsA method of photometric in vitro determination of the content of an analyte in a sample is disclosed. The sample is located in a measuring chamber which has a radiation path length and has at least one at least partially transparent wall part. The measuring chamber is in optical communication with an...http://www.google.com/patents/US5371020?utm_source=gb-gplus-sharePatent US5371020 - Method of photometric in vitro determination of the content of an analyte in a sampleAdvanced Patent SearchPublication numberUS5371020 APublication typeGrantApplication numberUS 08/050,100Publication dateDec 6, 1994Filing dateSep 17, 1991Priority dateSep 19, 1991Fee statusPaidAlso published asDE69221306D1, DE69221306T2, EP0605598A1, EP0605598B1, WO1993006456A1Publication number050100, 08050100, US 5371020 A, US 5371020A, US-A-5371020, US5371020 A, US5371020AInventorsPeter A. FrischaufOriginal AssigneeRadiometer A/SExport CitationBiBTeX, EndNote, RefManPatent Citations (16), Non-Patent Citations (5), Referenced by (42), Classifications (22), Legal Events (6) External Links: USPTO, USPTO Assignment, EspacenetMethod of photometric in vitro determination of the content of an analyte in a sample
US 5371020 AAbstract
1. A method of photometric in vitro determination of the content of an analyte in a sample, the method comprising:A) providing a measuring chamber for holding the sample, the measuring chamber comprising at least one partially transparent wall in optical communication with the sample, wherein the measuring chamber receives radiation through the at least one partially transparent wall and transmits the radiation over a path having a path length through the sample and out of the chamber through the at least one partially transparent wall, a shape of the measuring chamber being adjustable for varying the path length through the chamber; B) providing an optical system comprising a radiation source and a radiation detector; C) mounting the measuring chamber in optical communication with the radiation source for transmitting radiation into the sample through the at least one partially transparent wall, and mounting the measuring chamber in optical communication with the radiation detector for receiving the radiation transmitted through the sample; D) setting in a first measuring step a first unknown radiation path length through the measuring chamber, and transmitting radiation at at least two wavelengths from the radiation source through the measuring chamber and to the radiation detector; E) in a second measuring step, adjusting the shape of the measuring chamber and thereby setting a second unknown radiation path length across the measuring chamber, and transmitting radiation at the same at least two wavelengths as used during the first measuring step from the radiation source through the measuring chamber and to the radiation detector; and F) determining the analyte content of the sample from the absorbance of the sample detected in each of the measuring steps, the determination being independent of the change in path length, the determination being made by solving simultaneous equations. 2. The method according to claim 1 wherein the measuring chamber has optical transmission properties, the optical transmission properties being essentially independent of the shape adjustment of the measuring chamber.
FIGS. 1a and b show an embodiment of a measuring chamber device for use in determination of an analyte in a sample. The measuring chamber device, generally designated 4000, is part of an analyzer having an optical unit as further described below in connection with FIG. 4. The measuring chamber device 4000 consists of two identical halves 4001 and 4002. Said halves are made by means of injection moulding of a transparent "soft" plastic material, for example polyethylene terephthalate (PETP) of the type ARNITE� from AKZO, Arnhem, Holland. The two halves are assembled by pins 4005 and 4006 in the half 4001 engaging mating, not shown recesses in the half 4002, while not shown pins in the latter engage recesses 4007 and 4008 in the half 4001. Thereafter, the two halves are welded together by means of ultrasonic welding.
FIG. 4 shows a prototype of an optical unit 400 for use in the determination of an analyte in a sample. The sample is located in the measuring chamber device 4000, which is secured between a movable part 410 and a stationary part 412. The movable part 410 may be displaced along the centre line cl by means of an electromagnet 411. The optical unit 400 comprises a radiation source 401 in the form of a thermal radiation unit, more specifically a CrNi filament heated to a temperature of approx. 1050� C. The radiation source 401 is specially made for the present purpose by Applicant. The optical unit 400 further comprises a reference diode 413 of the type SFH 225 from Siemens, Munich, Germany, which regulates the radiation of the filament 401.
Radiation from the radiation source 401 is transmitted to a parabolic mirror 402 (φ 10 mm, f=3 mm) specially made for the present purpose by Applicant. The parabolic mirror 402 transmits the radiation from the filament 401 through a band-pass filter 403 (center value 4240 nm, half band width 200 nm) from Spektrogon, Taby, Sweden, and from there through the blood sample in the measuring chamber device 4000. From the measuring chamber device 4000, the radiation is transmitted through a slit 404 (0.5�3 mm) located on a sapphire window (φ3�0.4 mm), past a chopper 408, which by means of a DC motor 409 provides a chopper frequency of approx. 800 Hz. After passage of the chopper 408, the radiation falls onto an off-axis parabolic mirror 405 (f=65 mm) specially made by Applicant which reflects the radiation as a parallel pencil onto a grating 406 (a 300-lines grating optimized to approx. 4.3 μm) from Optometrics, Leeds, England. The grating 406 deflects the radiation back onto the parabolic mirror 405, which focuses the radiation onto a detector 407 from Hamamatsu, Hamamatsu City, Japan. The detector 407 comprises a diode array consisting of three PbSe-diodes (0.5�2 mm, center distance 1 mm) built-into a T05-housing with a band-pass filter (center value 4400 nm, half band width 650 nm). The diodes in the diode array 407 register the intensity of radiation at their respective of the three measuring wavelengths, which are separated by means of the grating 406 and the off-axis parabolic mirror 405. From the diodes electric signals representing the intensity of radiation incoming to the diodes are sent to a analog/digital converter (not shown) and from there to a data processing unit (not shown) performing the calculations of the content of CO2 in the sample on the basis of the signals received. FIGS. 5a and b show a partial section corresponding to FIG. 1c through a measuring chamber device 4000 according to the invention placed between the movable part 410 and the stationary part 412 of an optical unit for measuring of the CO2 content in a sample. As described in connection with FIG. 4 measuring radiation is transmitted through a band-pass filter 403 in the movable part 410, through the measuring chamber device 4000 to the slit 404 in the stationary part 412.
The measuring chamber device 40 comprises a body 41 consisting of two parts which after assembly form an internal continuous sample conduit 44 with a cross section of 0.3�2 mm. At the end of the body 41, where the inlet of the conduit is located, the body 41 is provided with a Luer cone 42 and thus adapted to be connected to a needle 43 of the type usually employed for blood sampling. At the end opposite the inlet opening, the conduit 44 is provided with a hydrophilic/hydrophobic filter 48, and at this end 49 the body 41 is adapted for coupling to a traditional plunger syringe which may serve as an sampling aid in special situations.
Al,&#955; =log (I.sub.&#955; /Il,&#955;)   (2)
Al,&#955; =Al,&#955; (H2 O)+Al,&#955; (CO2)+Al,&#955; (ib)+A.sub.&#955; (if)+A.sub.&#955; (ia)+A.sub.&#955; (io) (3)
A.sub.&#955; (CO2)=&#949;.sub.&#955; (CO2)�l�C(CO2)                (5)
C(CO2)=&#945;t (CO2)�pCO2    (7)
When introducing (7) into the second term of (6) the following expression is obtained: ##EQU4## where ε.sub.λ '(H2 O)=ε.sub.λ (H2 O)�C(H2 O);
ε.sub.λ '(CO2)=ε.sub.λ (CO2)�αt (CO2);
C'(CO2)=Δl�pCO2 ; and
C'(ib)=Δl�C (ib)
&#916;A.sub.&#955;1 =&#949;.sub.&#955;1 '(H2 O)�&#916;l+&#949;.sub.&#955;1 '(CO2)�C'(CO2)+&#949;.sub.&#955;1 (ib)�C'(ib)
&#916;A.sub.&#955;2 &#949;.sub.&#955;2 '(H2 O)�&#916;l+&#949;.sub.&#955;2 '(CO2)�C'(CO2 )+&#949;.sub.&#955;2 (ib)�C'(ib )
&#916;A.sub.&#955;3 =&#949;.sub.&#955;3 '(H2 O) �&#916;l+&#949;.sub.&#955;3 '(CO2)�C'(CO2)+&#949;.sub.&#955;3 (ib)�C'(ib                             (9)
pCO2 =C'(CO2)/&#916;l                           (10)
In the determination of the CO2 content in a sample it is essential to consider the absorption caused by other analytes in the sample (ελ(ib)�C'(ib)) - the particular values for ε(ib) and C'(ib) are unimportant. As mentioned previously, C'(ib) is only a theoretical value of no real physical importance. Therefore, the determination of the absorption coefficients ε.sub.λ1 (ib), ε.sub.λ2 (ib), ε.sub.λ3 (ib) is not a matter of determining these coefficients definitely, but determining the ratios among them.
The smallest of the absorption coefficients is set to 1.0�10-3 μm being of the same magnitude as the absorption coefficients for water, and the other coefficients are determined relatively thereto.
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS3740156 *Aug 11, 1971Jun 19, 1973Exxon Research Engineering CoPhotometric analyzer sampling cellUS3810695 *Dec 14, 1972May 14, 1974Gam RadFluid analyzer with variable light pathUS4509522 *Dec 15, 1981Apr 9, 1985The United States Of America As Represented By The Secretary Of The NavyInfrared optical measurement of blood gas concentrations and fiber optic catheterUS4762798 *Dec 31, 1985Aug 9, 1988Marshall Diagnostics, Inc.Measurement by changing optical density; glycohemogloben in blood for diabetes treatmentUS4786171 *Jul 29, 1986Nov 22, 1988Guided Wave, Inc.Spectral analysis apparatus and methodUS4873993 *Jul 13, 1987Oct 17, 1989Personal Diagnostics, Inc.CuvetteUS4980551 *Jan 5, 1990Dec 25, 1990National Research Council Canada Conseil National De Recherches CanadaNon-pressure-dependancy infrared absorption spectra recording, sample cellUS4997769 *Apr 13, 1990Mar 5, 1991Radiometer A/SMethod and an apparatus for determining blood componentsUS5099123 *May 23, 1990Mar 24, 1992Biosensors Technology, Inc.Method for determining by absorption of radiations the concentration of substances in absorbing and turbid matricesUS5139333 *Nov 8, 1989Aug 18, 1992Automatik Apparate-Maschinebau GmbhMeasuring cell for the spectral analysis of flowing media, in particular plastic meltsUS5149503 *Jul 22, 1988Sep 22, 1992Terumo Kabushiki KaishaApparatus for measuring hemoglobin concentration and oxygen saturation thereofUS5168367 *Jan 16, 1991Dec 1, 1992Rourke Patrick E OVariable path length spectrophotometric probeEP0253559A1 *Jul 8, 1987Jan 20, 1988C.R. Bard, Inc.Sensor for measuring the concentration of a gaseous component in a fluid by absorptionGB2160646A * Title not availableWO1990007106A1 *Dec 21, 1989Jun 28, 1990Radiometer AsA method of photometric in vitro determination of a blood gas parameter in a blood sampleWO1990007109A1 *Dec 21, 1989Jun 28, 1990Radiometer AsA method of photometric in vitro determination of the content of an analyte in a sample of whole blood* Cited by examinerNon-Patent CitationsReference1Hummel, D. O., "Atlas der Kunststoff-Analyse," Ban 1, Teil 1., Muchen: Carl Hauser Verlag, 1968 (with English Abstract).2 *Hummel, D. O., Atlas der Kunststoff Analyse, Ban 1, Teil 1., M chen: Carl Hauser Verlag, 1968 (with English Abstract).3 *PCT International Search Report for PCT Application No. PCT/DK92/00280 corresponding to the present application.4Skoog, D. A. West, D. M., "Fundamentals of Analytical Chemistry," 4th ed., pp. 501-524 Philadelphia: Saunders College Publishing, 1982.5 *Skoog, D. A. West, D. M., Fundamentals of Analytical Chemistry, 4th ed., pp. 501 524 Philadelphia: Saunders College Publishing, 1982.* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleUS5550630 *Mar 19, 1993Aug 27, 1996The United States Of America As Represented By The Secretary Of AgricultureSpectrophotometric method for structural analysis of organic compounds, polymers, nucleotides and peptidesUS6188474May 12, 1999Feb 13, 2001Bayer CorporationOptical spectroscopy sample cellUS6238625 *Dec 18, 1998May 29, 2001Korea Advanced Institute Of Science And TechnologyHigh concentrations of samples in solutions can be measured without dilution, by way of employing a newly designed cuvette holder in a spectrophotometer fitted to a reactor.US6246474 *Apr 29, 1998Jun 12, 2001Particle Measuring Systems, Inc.Method and apparatus for measurement of particle size distribution in substantially opaque slurriesUS6275290Apr 22, 1999Aug 14, 2001Particle Measuring Systems, Inc.Chemical mechanical planarization (CMP) slurry quality control process and particle size distribution measuring systemsUS6605471May 27, 1997Aug 12, 2003Radiometer Medical A/SDiscarding holder; analyzing blood, urine; medical equipmentUS6771993Aug 15, 2002Aug 3, 2004Optiscan Biomedical CorporationSample adapterUS6958809Jan 21, 2002Oct 25, 2005Optiscan Biomedical CorporationReagent-less whole-blood glucose meterUS6983177Jan 6, 2003Jan 3, 2006Optiscan Biomedical CorporationLayered spectroscopic sample element with microporous membraneUS6989891Aug 14, 2002Jan 24, 2006Optiscan Biomedical CorporationDevice and method for in vitro determination of analyte concentrations within body fluidsUS7016087Aug 8, 2001Mar 21, 2006Becton Dickinson And CompanyPhoton efficient scannerUS7050157Jul 19, 2002May 23, 2006Optiscan Biomedical Corp.Reagent-less whole-blood glucose meterUS7061593Aug 14, 2002Jun 13, 2006Optiscan Biomedical Corp.Device and method for in vitro determination of analyte concentrations within body fluidsUS7271912Apr 15, 2004Sep 18, 2007Optiscan Biomedical CorporationMethod of determining analyte concentration in a sample using infrared transmission dataUS7480032Jan 23, 2006Jan 20, 2009Optiscan Biomedical CorporationDevice and method for in vitro determination of analyte concentrations within body fluidsUS7494620Oct 29, 2004Feb 24, 2009Bayer Healthcare LlcFormats for optical analysis and methods of manufacturing the sameUS7593108Aug 20, 2007Sep 22, 2009Optiscan Biomedical CorporationMethod of determining analyte concentration in a sample using infrared transmission dataUS7722537Dec 21, 2005May 25, 2010Optiscan Biomedical Corp.Method and apparatus for detection of multiple analytesUS7731897Oct 15, 2008Jun 8, 2010Bayer Healthcare LlcFormats for optical analysis and methods of manufacturing the sameUS7738085Dec 23, 2008Jun 15, 2010Optiscan Biomedical CorporationDevice and method for in vitro determination of analyte concentration within body fluidsUS7759651Apr 19, 2005Jul 20, 2010Trojan TechnologiesOptical radiation sensor system and method for measuring radiation transmittance of a fluidUS7763454Jul 9, 2004Jul 27, 2010Church & Dwight Co., Inc.Electronic analyte assaying deviceUS7796261Dec 1, 2005Sep 14, 2010Foss Analytical A/SSpectrophotometerUS7872734Jun 14, 2010Jan 18, 2011Optiscan Biomedical CorporationIn vitro determination of analyte levels within body fluidsUS7961310 *Jul 9, 2009Jun 14, 2011Durasens, LLCTransmission liquid flow cell with increased internal flow ratesUS7999927Jan 14, 2011Aug 16, 2011Optiscan Biomedical CorporationIn vitro determination of analyte levels within body fluidsUS8140140Dec 21, 2005Mar 20, 2012Optiscan Biomedical CorporationAnalyte detection system for multiple analytesUS8197770Jan 26, 2009Jun 12, 2012Optiscan Biomedical CorporationFluid handling cassette having a spectroscopic sample cellUS8226904Oct 29, 2004Jul 24, 2012Bayer Healthcare LlcOptical format with platform-and-well constructionUS8597190Dec 13, 2010Dec 3, 2013Optiscan Biomedical CorporationMonitoring systems and methods with fast initializationUS8623635Jun 3, 2010Jan 7, 2014Church & Dwight Co., Inc.Electronic analyte assaying deviceUS8722395Jun 3, 2010May 13, 2014Church & Dwight Co., Inc.Electronic analyte assaying deviceUS8906697Jun 23, 2005Dec 9, 2014Chemometec A/SMethod for the assessment of particles and a system and device for use in the methodUSRE39783Aug 12, 2003Aug 21, 2007Particle Measuring Systems, Inc.Chemical mechanical planarization (CMP) slurry quality control process and particle size distribution measuring systemsCN101069085BDec 1, 2005Jul 14, 2010福斯分析公司SpectrophotometerDE102008027085A1 *Jun 5, 2008May 20, 2010Gesellschaft zur F�rderung der Analytischen Wissenschaften e.V.Device for analysis of liquid sample, has radiation source, flexible measuring cell, in which sample is introduced, radiation detector and evaluation unitDE102008027085B4 *Jun 5, 2008Jul 15, 2010Leibniz-Institut f�r Analytische Wissenschaften-ISAS-e.V.Vorrichtung zur Analyse einer fl�ssigen Probe sowie Verfahren zur Analytbestimmung in einem Dialysat mit einer solchen VorrichtungEP1956359A1 *Oct 29, 2004Aug 13, 2008Bayer Healthcare, LLCFormats for optical analysis and methods of manufacturing the sameWO2004034038A1 *Jul 24, 2003Apr 22, 2004Council Cent Lab Res CouncilsSample cellWO2005043133A1 *Oct 29, 2004May 12, 2005Bayer Healthcare LlcFormats for optical analysis and methods of manufacturing the sameWO2006058741A1 *Dec 1, 2005Jun 8, 2006Foss Analytical AsSpectrophotometerWO2014097141A1 *Dec 17, 2013Jun 26, 2014Radiometer Medical ApsAn apparatus for detecting a component in a sample* Cited by examinerClassifications U.S. Classification436/165, 356/39, 356/320, 436/164, 356/246, 436/68, 422/82.09, 356/434, 422/82.05, 356/440International ClassificationG01N21/31, G01N21/05, G01N21/03, G01N35/10Cooperative ClassificationG01N2021/0364, G01N2021/3129, G01N21/0303, G01N21/314, G01N2035/1062, G01N2201/0662, G01N2021/036European ClassificationG01N21/03ALegal EventsDateCodeEventDescriptionJun 6, 2006FPAYFee paymentYear of fee payment: 12Jun 25, 2002REMIMaintenance fee reminder mailedMay 17, 2002FPAYFee paymentYear of fee payment: 8May 26, 1998FPAYFee paymentYear of fee payment: 4May 30, 1995CCCertificate of correctionJun 24, 1993ASAssignmentOwner name: RADIOMETER A/SFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:FRISCHAUF, PETER AAGE;REEL/FRAME:006585/0578Effective date: 19930517RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services