Source: http://www.google.com/patents/US6090347?dq=6,970,917
Timestamp: 2013-12-05 20:31:25
Document Index: 520452719

Matched Legal Cases: ['art 11', 'art 12', 'art 11', 'art 13', 'art 12', 'art 11', 'art 13', 'art 12', 'art 13', 'art 11', 'art 11', 'art 13', 'art 11', 'art 13']

Patent US6090347 - Test kit and use thereof - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Advanced Patent Search | Sign inAdvanced Patent SearchPatentsThe test kit includes a substrate and, welded or bonded thereto, a plastic sheet having blisters. One of the blisters is shaped as a siphon. The other blisters act as reaction vessels and also serve to receive and store a reagent. The test kit may contain a test strip in one of the blisters. The test...http://www.google.com/patents/US6090347?utm_source=gb-gplus-sharePatent US6090347 - Test kit and use thereofPublication numberUS6090347 APublication typeGrantApplication numberUS 09/142,381PCT numberPCT/CH1997/000121Publication dateJul 18, 2000Filing dateMar 21, 1997Priority dateMar 22, 1996Fee statusLapsedAlso published asDE59705773D1, EP0888191A1, EP0888191B1, WO1997035663A1Publication number09142381, 142381, PCT/1997/121, PCT/CH/1997/000121, PCT/CH/1997/00121, PCT/CH/97/000121, PCT/CH/97/00121, PCT/CH1997/000121, PCT/CH1997/00121, PCT/CH1997000121, PCT/CH199700121, PCT/CH97/000121, PCT/CH97/00121, PCT/CH97000121, PCT/CH9700121, US 6090347 A, US 6090347A, US-A-6090347, US6090347 A, US6090347AInventorsGabriel EmodiOriginal AssigneeIntex Pharmaceutische Produkte AgPatent Citations (23), Referenced by (1), Classifications (24), Legal Events (5) External Links: USPTO, USPTO Assignment, EspacenetTest kit and use thereofUS 6090347 AAbstract The test kit includes a substrate and, welded or bonded thereto, a plastic sheet having blisters. One of the blisters is shaped as a siphon. The other blisters act as reaction vessels and also serve to receive and store a reagent. The test kit may contain a test strip in one of the blisters. The test kit is particularly suited for carrying out immunological tests, whereby the siphon markedly facilitates the procedure during the washing step.
What is claimed is: 1. A test kit for carrying out analytical tests, the test kit comprising a water-impermeable substrate and, bonded or welded thereto, a transparent plastic sheet including blisters arranged parallel to one another, wherein at least one blister includes an elongate container having an S-shaped part and a rinsing part connected to the elongate container by the S-shaped part, the elongate container and the rinsing part together functioning as a siphon.
AREA OF THE INVENTION The present invention relates to test kits with test vessels which are formed by appropriate shaping of a plastic sheet and welding of this shaped sheet to a substrate, and to the use thereof for carrying out analytical tests, in particular immunological tests.
PRIOR ART The blister technique has already been employed in analysis for carrying out tests of various types. In the literature on such tests, the blisters are referred to not by their modern name but as bags, envelopes and the like.
DESCRIPTION OF THE INVENTION The object is achieved according to the invention by a test kit consisting of a water-impermeable substrate and, bonded or welded thereto, a transparent plastic sheet which is shaped to one or more blisters arranged parallel to one another, this test kit being characterized in that one blister is shaped so that it is able to act as a siphon.
DESCRIPTION OF PREFERRED EMBODIMENTS In a first embodiment of the invention (FIG. 1), the kit comprises precisely just the one siphon blister 1 which is open at both ends. Its upward-pointing branch consists of a container part 11 and, adjoining at the top, a removal part 12 (whose cross-section may be increased by comparison with that of the container part in order to facilitate manipulation of test strips). The elongate, shallow container part 11 of the siphon blister 1 is extended and curved in an S shape by a rinsing part 13 at the end opposite to the removal part 12, so that container part 11 and rinsing part 13 together form a siphon. The siphon blister is shown in a form open at the removal part 12 and at the end of the rinsing part 13, because in this case it is mostly used only for carrying out the washing step and thus sterility of the siphon blister is unnecessary. For the washing (FIGS. 2a, 2b, 2c), the test strip 2 is introduced into the container part 11 and, with the container part 11 in at least approximately vertical alignment, washing solution is added and can then flow out again through the rinsing part 13. The washing of the test strip 2 takes place with the washing solution either flowing through or stationary, and in the latter case the amount of washing solution initially introduced into the container part 11 is such that no solution escapes through the rinsing part 13 (FIG. 2a). Then, in a second step, further washing solution (FIG. 2b) is introduced to empty the siphon (FIG. 2c) through the siphon effect. This washing method can be applied to all embodiments of the test kit according to the invention.
EXAMPLE 1 Sample from Patient N.N. Determination of Total IgE Using a Blister Card According to the Invention The reagents of the total IgE test kit are brought to room temperature (RT=15 to 30 removed and cut open along the lines 3 and 3', and the name of the patient (N.N.) is written on the blister card and an associated patient's card. The total IgE test strip contains the negative control at position 1, the fields for the patient's sample at positions 2 and 3, and the IgE standards 400 kU/l, 100 kU/l, 20 kU/l and 5 kU/l at position 4 to 7.
A pipette is used to introduce 300 μl of patient's serum into the first blister 4 of the blister card, and a dropper bottle is used to add 300 μl of anti-human IgE-peroxidase test solution [(monoclonal mouse) anti-human IgE antibody-peroxidase conjugate (supplied by SBAI, Prod. No. 9160-05) in tris/HCl buffer of pH 7.6 with the addition of 500 ml/l heat-inactivated (56 and 0.16 ml/l Kathon 886 WT, 14%)] to the serum which is present. The two solutions are thoroughly mixed by moving the total IgE test strip up and down in the blister several times; the test strip is then incubated in the solution at RT for one hour.
The test strip 2 is incubated in the siphon blister 1 filled with fresh distilled water at 22 excess chromogen/hydrogen peroxide solution.
EXAMPLE 2 Sample from Patient N.N. Detection of Inhaled Allergen--Specific IgE Using a Blister Card According to the Invention for Serological Diagnosis of an Allergic Disorder: The reagents of the IgE inhaled allergen test kit are brought to room temperature (22 removed and cut open along lines 3 and 3', and the name of the patient is written on the blister card and an associated patient's card. The test strip for inhaled allergen IgE contains: position 1=negative control, position 2=positive control, position 3=Timothygrass, position 4=artemisia, position 5=ragweed, position 6=rye, position 7=birch, position 8=alternaria, position 9=Dermatophagoides pteronyssinus, position 10=Dermatophagoides farinae, position 11=dog epithelium and position 12=cat epithelium.
A dropper bottle is used to add anti-human IgE-peroxidase test solution [(monoclonal mouse) anti-human IgE antibody-peroxidase conjugate (supplied by SBAI, Prod. No. 9160-05) in tris/HCl buffer of pH 7.6 with addition of 500 ml/l heat-inactivated (56 phenol and 0.16. ml/l Kathon 886 WT, 14%)] up to the upper mark in the second blister 5 of the blister card.
The washed test strip is incubated in blister 5 at 22 hour.
EXAMPLE 3 Sample from Patient N.N. Detection of Food Allergen--Specific IgE Using a Blister Card According to the Invention for Serological Diagnosis of an Allergic Disorder: The reagents of the IgE food allergen test kit are brought to room temperature (RT/30 min), a blister card as shown in FIG. 5b is removed and cut open along lines 3 and 3', and the name of the patient is written on the blister card and an associated patient's card. The test strip for food allergen IgE contains: position 1=negative control, position 2=positive control, position 3=wheat, position 4=soya beans, position 5=maize, position 6=hazelnut, position 7=peanut, position 8=milk, position 9=egg, position 10=cod, position 11=tomato and position 12=orange.
A dropper bottle is used to add anti-human IgE-peroxidase test solution [(monoclonal mouse) anti-human IgE antibody-peroxidase conjugate (supplied by SBAI, Prod. No. 9160-05) in tris/HCl buffer of pH 7.6 with addition of 500 ml/l heat-inactivated (56 phenol and 0.16 ml/l Kathon 886 WT, 14%)] up to the upper mark in the second blister 5 of the blister card. The washed test strip is removed from the siphon blister and incubated in blister 5 at RT for one hour.
The test strip is incubated in the siphon blister filled with fresh distilled water at 22 chromogen.
EXPLANATION OF THE FIGURES FIG. 1 shows a test kit according to the invention with precisely just one siphon blister 1 which is open at both ends.
Patent CitationsCited PatentFiling datePublication dateApplicantTitleUS3660033 *Sep 29, 1969May 2, 1972Leroy L SchwartzDisposable specimen collection and analysis bagUS4065263 *Jun 14, 1976Dec 27, 1977Woodbridge Iii Richard GAnalytical test strip apparatusUS4343709 *Dec 9, 1980Aug 10, 1982Tetsuo MatsumotoMethod and device for separately collecting and discharging components of liquid in centrifugal rotorUS4459710 *Oct 18, 1982Jul 17, 1984The Drackett CompanyPassive dispenserUS4503864 *Sep 30, 1982Mar 12, 1985Powers Jerry GUrine specimen collection apparatus with a separable compartmentUS4790833 *Sep 26, 1986Dec 13, 1988Coloplast A/SBag of plastic film for collecting discharges from human or animal bodies via drainsUS4913161 *Dec 23, 1987Apr 3, 1990The Kendall CompanyBag-tilt indicator on urine bagUS4965047 *Feb 17, 1988Oct 23, 1990Cmb Foodcan P.L.C.Analytical test stripUS5087251 *Jan 9, 1991Feb 11, 1992Heyman Arnold MEntirely disposable unitary urine draining bag and support harness systemUS5263946 *Feb 13, 1992Nov 23, 1993Sierra Laboratories, Inc.Latex urine container having odor impermeable treatment and provided with integral strap holdersUS5354538 *Oct 15, 1992Oct 11, 1994Bunce Roger ALiquid transfer devicesUS5422271 *Nov 20, 1992Jun 6, 1995Eastman Kodak CompanyNucleic acid material amplification and detection without washingUS5643236 *Apr 12, 1995Jul 1, 1997Hadley; Jack D.Leg underpant for supporting fluid collection bagUS5731212 *Dec 20, 1994Mar 24, 1998International Technidyne CorporationTest apparatus and method for testing cuvette accommodated samplesUS5770441 *Sep 30, 1996Jun 23, 1998Lipton; StewartMethods, apparatuses and kits for the growth and/or identification of microorganismsUS5811296 *Dec 20, 1996Sep 22, 1998Johnson & Johnson Clinical Diagnostics, Inc.Blocked compartments in a PCR reaction vesselUS5842326 *Jun 16, 1994Dec 1, 1998Farco-Pharma Gesellschaft Mit Beschrankter Haftung Pharmazeutische PraparateMethod for fabricating a sterile ready-pack and a container for such a ready-packUS5843793 *Oct 10, 1996Dec 1, 1998Johnson & Johnson Clinical Diagnostics, Inc.Container for staining of cells and tissues in combination with a roller and a supportUS5846488 *Jun 26, 1997Dec 8, 1998Hawaii Chemtect InternationalField kit for detecting analytesUS5888832 *Jul 21, 1998Mar 30, 1999Hawaii Chemtect InternationalMethod for using a field kit for detecting analytesEP0139373A1 *Aug 10, 1984May 2, 1985The Regents Of The University Of CaliforniaMultiple immunoassay systemWO1991016086A1 *Apr 12, 1991Oct 31, 1991Target Research IncMulticompartment biological fluid specimen collection bagWO1993007474A1 *Sep 30, 1992Apr 15, 1993Hawaii Chemtect IntField kit for detecting analytes* Cited by examinerReferenced byCiting PatentFiling datePublication dateApplicantTitleEP2248590A1 *Dec 12, 2005Nov 10, 2010Bayer Healthcare LLCSelf-contained test sensor* Cited by examinerClassifications U.S. Classification422/401, 422/162, 435/288.2, 206/569, 422/68.1, 206/532, 436/541, 435/287.1, 435/287.6, 206/469, 436/183, 436/513, 435/288.5, 422/430International ClassificationB01L99/00, G01N33/543, G01N1/10, B01L3/00Cooperative ClassificationB01L2300/0816, B01L3/505, B01L2300/0663, B01L9/54European ClassificationB01L3/505, B01L9/54Legal EventsDateCodeEventDescriptionSep 9, 2008FPExpired due to failure to pay maintenance feeEffective date: 20080718Jul 18, 2008LAPSLapse for failure to pay maintenance feesJan 28, 2008REMIMaintenance fee reminder mailedJan 16, 2004FPAYFee paymentYear of fee payment: 4Sep 4, 1998ASAssignmentOwner name: INTEX PHARMAZEUTISCHE PRODUKTE AG, SWITZERLANDFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:EMODI, GABRIEL;REEL/FRAME:009627/0561Effective date: 19980814RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services©2012 Google