Source: http://www.science.gov/topicpages/a/agreements+require+extensive.html
Timestamp: 2016-10-22 11:50:16
Document Index: 384616262

Matched Legal Cases: ['§ 1', '§ 73', 'art 214', '§ 439', 'art 215', 'art 251', 'arts 264']

agreements require extensive: Topics by Science.gov
Sample records for agreements require extensive
42 CFR 442.16 - Extension of agreement for ICFs/MR.
... expiration date specified in the agreement if it receives written notice from the survey agency, before the expiration date of the agreement, that extension will not jeopardize the patients' health and safety, and— (a...; or (b) Is needed because it is impracticable to determine, before the expiration date, whether...
78 FR 16275 - Extension of the Duration of Programmatic Agreements Based on the Department of Energy Prototype...
... 22, 2013, for a one-week comment period (78 FR 12336-12337). In accordance with 36 CFR 800.14(e), the... HISTORIC PRESERVATION Extension of the Duration of Programmatic Agreements Based on the Department of... on Historic Preservation. ACTION: The Advisory Council on Historic Preservation has issued a...
Moderators of informant agreement in the assessment of adolescent psychopathology: extension to a forensic sample.
Penney, Stephanie R; Skilling, Tracey A
A well-documented finding in developmental psychopathology research is that different informants often provide discrepant ratings of a youth's internalizing and externalizing problems. The current study examines youth- and parent-based moderators (i.e., youth age, gender, and IQ; type of psychopathology; offense category; psychopathic traits; parental education, income, and stress) of informant discrepancies in a sample of young offenders and compares the utility of youth and caregiver reports against relevant clinical outcomes. Results indicate that gender moderated the discrepancy between informant reports of somatic complaints, while parenting stress moderated the discrepancies across reports of internalizing and externalizing psychopathology. Variables unique to the forensic context (e.g., offense category) were found to moderate cross-informant discrepancies in reports of internalizing and externalizing psychopathology. Further, youth self-reports of internalizing symptoms predicted a clinician-generated diagnosis of a mood disorder, while caregiver reports of aggressive behaviors predicted the presence of an externalizing diagnosis. Results highlight the importance of assessing informant agreement in the context of forensic assessment and raise questions surrounding the optimal use of informant data in this setting. PMID:21966931
Manned geosynchronous mission requirements and systems analysis study extension
Turnaround requirements for the manned orbital transfer vehicle (MOTV) baseline and alternate concepts with and without a space operations center (SOC) are defined. Manned orbital transfer vehicle maintenance, refurbishment, resupply, and refueling are considered as well as the most effective combination of ground based and space based turnaround activities. Ground and flight operations requirements for abort are identified as well as low cost approaches to space and ground operations through maintenance and missions sensitivity studies. The recommended turnaround mix shows that space basing MOTV at SOC with periodic return to ground for overhaul results in minimum recurring costs. A pressurized hangar at SOC reduces labor costs by approximately 50%.
37 CFR 1.730 - Applicant for extension of patent term; signature requirements.
... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Applicant for extension of patent term; signature requirements. 1.730 Section 1.730 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES Adjustment and Extension of Patent Term...
... 37 Patents, Trademarks, and Copyrights 1 2013-07-01 2013-07-01 false Applicant for extension of patent term; signature requirements. 1.730 Section 1.730 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES Adjustment and Extension of Patent Term...
75 FR 70948 - Proposed Extension of the Approval of Information Collection Requirements
... of Federal Contract Compliance Programs Proposed Extension of the Approval of Information Collection Requirements AGENCY: Office of Federal Contract Compliance Programs, Labor. ACTION: Notice. SUMMARY: The... be properly assessed. Currently, the Office of Federal Contract Compliance Programs is...
75 FR 22844 - Construction Fall Protection Systems Criteria and Practices and Training Requirements; Extension...
... 1995 (44 U.S.C. 3506 et seq.) and Secretary of Labor's Order No. 5-2007 (72 FR 31160). Signed at... Occupational Safety and Health Administration Construction Fall Protection Systems Criteria and Practices and Training Requirements; Extension of the Office of Management and Budget's (OMB) Approval of...
77 FR 74775 - Extension of Dates for Certain Requirements and Amendment of Form 19b-4
... (77 FR 73302), in FR Doc. 2012-29712, the following corrections are made to page 73305: 1. In the... 240 RIN 3235-AK87 Extension of Dates for Certain Requirements and Amendment of Form 19b-4 AGENCY... December 10, 2013, and amend the General Instructions to Form 19b-4 to clarify the process for...
... 37 Patents, Trademarks, and Copyrights 1 2014-07-01 2014-07-01 false Formal requirements for application for extension of patent term; correction of informalities. 1.740 Section 1.740 Patents, Trademarks... Review § 1.740 Formal requirements for application for extension of patent term; correction...
Zallen, J A; Kirch, S A; Bargmann, C I
Over half of the neurons in Caenorhabditis elegans send axons to the nerve ring, a large neuropil in the head of the animal. Genetic screens in animals that express the green fluorescent protein in a subset of sensory neurons identified eight new sax genes that affect the morphology of nerve ring axons. sax-3/robo mutations disrupt axon guidance in the nerve ring, while sax-5, sax-9 and unc-44 disrupt both axon guidance and axon extension. Axon extension and guidance proceed normally in sax-1, sax-2, sax-6, sax-7 and sax-8 mutants, but these animals exhibit later defects in the maintenance of nerve ring structure. The functions of existing guidance genes in nerve ring development were also examined, revealing that SAX-3/Robo acts in parallel to the VAB-1/Eph receptor and the UNC-6/netrin, UNC-40/DCC guidance systems for ventral guidance of axons in the amphid commissure, a major route of axon entry into the nerve ring. In addition, SAX-3/Robo and the VAB-1/Eph receptor both function to prevent aberrant axon crossing at the ventral midline. Together, these genes define pathways required for axon growth, guidance and maintenance during nervous system development. PMID:10409513
Wang, Wei; Rai, Asit; Hur, Eun-Mi; Smilansky, Zeev; Chang, Karen T; Min, Kyung-Tai
Local information processing in the growth cone is essential for correct wiring of the nervous system. As an axon navigates through the developing nervous system, the growth cone responds to extrinsic guidance cues by coordinating axon outgrowth with growth cone steering. It has become increasingly clear that axon extension requires proper actin polymerization dynamics, whereas growth cone steering involves local protein synthesis. However, molecular components integrating these two processes have not been identified. Here, we show that Down syndrome critical region 1 protein (DSCR1) controls axon outgrowth by modulating growth cone actin dynamics through regulation of cofilin activity (phospho/dephospho-cofilin). Additionally, DSCR1 mediates brain-derived neurotrophic factor-induced local protein synthesis and growth cone turning. Our study identifies DSCR1 as a key protein that couples axon growth and pathfinding by dually regulating actin dynamics and local protein synthesis. PMID:27185837
Qaisi, Mohammed; Loeb, Matthew; Montague, Lindsay; Caloss, Ron
Brown tumor of hyperparathyroidism (BTHPT) is rare in the United States and not frequently seen in clinical practice. This is likely because early diagnosis and prompt treatment of this disease process prevent the progression and development of BTHPT. Conversely, BTHPT is more common in underdeveloped countries where fewer patients have access to health care and hyperparathyroidism (HPT) goes untreated. It has been reported that the incidence of BTHPT in underdeveloped countries can be as high as 58 to 69 percent in patients with primary HPT. We present a case report of a patient in the United States with a large mandibular BTHPT requiring an extensive resection in the setting of secondary HPT. Despite being rare in this country, it is important for nephrologists, primary care physicians, and oral health care providers to be able to recognize this entity, so that intervention may be rendered early. PMID:26413096
75 FR 4585 - Proposed Extension of the Approval of Information Collection Requirements
... of Workers' Compensation Programs Proposed Extension of the Approval of Information Collection... respondents can be properly assessed. Currently, the Office of Workers' Compensation Programs is soliciting... of students. Type of Review: Extension. Agency: Office of Workers' Compensation Programs....
47 CFR 73.3549 - Requests for extension of time to operate without required monitors, indicating instruments, and...
... without required monitors, indicating instruments, and EAS encoders and decoders. 73.3549 Section 73.3549... required monitors, indicating instruments, and EAS encoders and decoders. Requests for extension of... decoders for monitoring and generating the EAS codes and Attention Signal should be made to the FCC...
47 CFR 1.549 - Requests for extension of authority to operate without required monitors, indicating instruments...
... 47 Telecommunication 1 2010-10-01 2010-10-01 false Requests for extension of authority to operate without required monitors, indicating instruments, and EBS Attention Signal devices. 1.549 Section 1.549... required monitors, indicating instruments, and EBS Attention Signal devices. See § 73.3549....
75 FR 7292 - Proposed Extension of the Approval of Information Collection Requirements
... of Workers' Compensation Programs Proposed Extension of the Approval of Information Collection... respondents can be properly assessed. Currently, the Office of Workers' Compensation Programs is soliciting... (mail, fax, or e-mail). SUPPLEMENTARY INFORMATION: I. Background: The Office of Workers'...
78 FR 35981 - Proposed Extension of the Approval of Information Collection Requirements
... of Workers' Compensation Programs Proposed Extension of the Approval of Information Collection... respondents can be properly assessed. Currently, the Office of Workers' Compensation Programs is soliciting..., fax, or Email). SUPPLEMENTARY INFORMATION: I. Background: The Office of Workers' Compensation...
78 FR 64984 - Extension of the Approval of Information Collection Requirements
... changed by a Notice of Proposed Rulemaking published December 27, 2011 (76 FR 81199-200). 44 U.S.C. 3506(c... Domestic Service; Final Rule, in the Federal Register on October 1, 2013 (78 FR 60454). At the time of... information collection (78 FR 60497). Notice is hereby given that the OMB has approved the extension of...
46 CFR 327.20 - Admiralty Jurisdiction Extension Claims: Required claims.
... United States of America, acting by and through the Maritime Administration, with respect to such.... 327.20 Section 327.20 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION A-NATIONAL...) Pursuant to 46 U.S.C. 30101(c) of the Admiralty Extension Act (AEA), administrative claims involving...
XIer2 is required for convergent extension movements during Xenopus development
Hong, Sung-Kook; Tanegashima, Kosuke; Dawid, Igor B.
Immediate early response 2 (Ier2) is a downstream target of FGF signaling. In zebrafish, Ier2 is involved in left-right asymmetry establishment and in convergent extension movements. We isolated the Xenopus ier2 gene based on sequence similarity searches using multiple vertebrate species. Xenopus Ier2 has high homology in the N-terminal region to other vertebrate Ier2 proteins, and Xier2 transcripts were observed from oocytes through larval stages. Except for the maternal expression of xier2, the expression of this gene in the marginal region at gastrulation and in somites and the notochord at later stages is similar to the expression pattern of zebrafish ier2. XIer2 knockdown using antisense morpholinos resulted in defects of convergent extension leading to severe neural tube defects; overexpression of Ier2 showed similar albeit milder phenotypes. Assays in animal cap explants likewise showed inhibition of elongation after blocking XIer2 expression. These results indicate that Xenopus Ier2 is essential for the execution of convergent extension movements during early Xenopus development. PMID:22252488
Hong, Sung-Kook; Tanegashima, Kosuke; Dawid, Igor B
Immediate early response 2 (Ier2) is a downstream target of fibroblast growth factor (FGF) signaling. In zebrafish, Ier2 is involved in left-right asymmetry establishment and in convergent extension movements. We isolated the Xenopus ier2 gene based on sequence similarity searches using multiple vertebrate species. Xenopus Ier2 has high homology in the N-terminal region to other vertebrate Ier2 proteins, and Xier2 transcripts were observed from oocytes through larval stages. Except for the maternal expression of xier2, the expression of this gene in the marginal region at gastrulation and in somites and the notochord at later stages is similar to the expression pattern of zebrafish ier2. XIer2 knockdown using antisense morpholinos resulted in defects of convergent extension leading to severe neural tube defects; overexpression of Ier2 showed similar, albeit milder phenotypes. Assays in animal cap explants likewise showed inhibition of elongation after blocking XIer2 expression. These results indicate that Xenopus Ier2 is essential for the execution of convergent extension movements during early Xenopus development. PMID:22252488
77 FR 67026 - Proposed Extension of the Approval of Information Collection Requirements
... determined by the Department of Labor, to laborers and mechanics on most federally financed or assisted... involving the employment of laborers or mechanics. The requirements of this information collection...
78 FR 21159 - Additional Requirements for Special Dipping and Coating Operations (Dip Tanks); Extension of the...
... Paperwork Reduction Act of 1995 (44 U.S.C. 3506 et seq.) and Secretary of Labor's Order No. 1-2012 (77 FR... Occupational Safety and Health Administration Additional Requirements for Special Dipping and Coating... Coating Operations (Dip Tanks) (29 CFR 1910.126(g)(4)). DATES: Comments must be submitted...
76 FR 27670 - Proposed Extension of the Approval of Information Collection Requirements; Comment Request
... also require affirmative action to ensure that equal employment opportunities are available regardless... 503 of the Rehabilitation Act of 1973, as amended, 29 U.S.C. 793; and The affirmative action...-discrimination and affirmative action provisions of the three legal authorities it administers. Type of...
75 FR 49865 - Extension of Comment Period; Airplane and Engine Certification Requirements in Supercooled Large...
... Requirements in Supercooled Large Drop, Mixed Phase, and Ice Crystal Icing Conditions'' (75 FR 37311, Docket No... for the NPRM published on June 29, 2010 (75 FR 37311) was scheduled to close on August 30, 2010, and... (65 FR 19477-78) or you may visit http://DocketsInfo.dot.gov . Docket: To read background documents...
78 FR 58267 - Notice of Request for Extension of Approval of an Information Collection; Requirements for...
... Collection; Requirements for Poultry and Hatching Eggs for Export AGENCY: Animal and Plant Health Inspection... with the export of poultry and hatching eggs from the United States. DATES: We will consider all...: For information on the export of poultry and hatching eggs from the United States, contact Dr....
76 FR 34635 - Medical Certification Requirements as Part of the Commercial Driver's License (CDL); Extension of...
... Management System published in the Federal Register on January 17, 2008, (73 FR 3316) or you may visit http...,'' issued on December 1, 2008, (73 FR 73096-73097) is also applicable to this rule. Background On December 1... both SDLAs and employers also were implemented (73 FR 73096-73128). These requirements, for the...
.... DATES: Comment Due Date: For the proposed rule published on September ] 13, 2013 (78 FR 56625), the... Service at 800-877-8339. SUPPLEMENTARY INFORMATION: On September 13, 2013 (78 FR 56625), HUD published a... URBAN DEVELOPMENT 24 CFR Part 214 Housing Counseling Program: New Certification Requirements;...
... the NPRM published on November 13, 2012 (77 FR 67584), was scheduled to close on February 11, 2013... Statement can be found in the Federal Register published on April 11, 2000 (65 FR 19477-19478), as well as... Maintenance Requirements'' (77 FR 67584). Comments to that document were to be received on or before...
78 FR 72715 - Proposed Revision and Extension of the Approval of Information Collection Requirements
..., which requires or involves the employment of mechanics and/ or laborers, shall contain a provision stating the minimum wages to be paid various classes of laborers and mechanics that were determined by the Secretary of Labor to be prevailing for the corresponding classes of laborers and mechanics employed...
75 FR 36444 - Proposed Extension of the Approval of Information Collection Requirements
76 FR 48181 - Proposed Extension of the Approval of Information Collection Requirements
... Management and Budget (OMB) approval of the Information Collection: Davis-Bacon Certified Payroll. A copy of.... Regulations 29 CFR 5.5 (a)(3)(ii)(A) requires contractors to submit weekly a copy of all payrolls to the... contract, the contractor will submit the payrolls to the applicant, sponsor, or owner, as the case may...
Manned geosynchronous mission requirements and systems analysis study extension. Volume 1: Executive summary
A study was performed to determine the types of manned missions that will likely be performed in the late 1980's or early 1990's timeframe, to define MOTV configurations which satisfy these missions requirements, and to develop a program plan for its development. Twenty generic missions were originally defined for MOTV but, to simplify the selection process, five of these missions were selected as typical and used as Design Reference Missions. Systems and subsystems requirements were re-examined and sensitivity analyses performed to determine optimum point designs. Turnaround modes were considered to determine the most effective combination of ground based and spaced based activities. A preferred concept for the crew capsule and for the mission mode was developed.
Smith, D J; Payton, M A
A strain of Aspergillus nidulans carrying a temperature-sensitive mutation in the manA gene produces cell walls depleted of D-mannose and forms hyphal tip balloons at the restrictive temperature (B.P. Valentine and B.W. Bainbridge, J. Gen. Microbiol. 109:155-168, 1978). We have isolated and characterized the manA gene and physically located it between 3.5 and 5.5 kb centromere distal of the riboB locus on chromosome VIII. The manA gene contains four introns and encodes a 50.6-kDa protein which has significant sequence identity to type I phosphomannose isomerase proteins from other eukaryotes. We have constructed by integrative transformation a null mutation in the manA gene which can only be maintained in a heterokaryotic strain with wild-type manA+ nuclei. Thus, a manA null mutation is lethal in A. nidulans. The phenotype of the mutation was analyzed in germinating conidia. Such conidia are able to commence germination but swell abnormally, sometimes producing a misshapen germ tube, before growth ceases. The reason for the lethality is probably the lack of synthesis of mannose-containing cell wall polymers that must be required for normal cell wall development in growing hyphae. Images PMID:8065336
Hufnal, Bryce; Farber, Robert; Munkácsy, Erin; Rodriguez, Amanda; Dillow, Andy; Kahlig, Erynn; Rea, Shane L.
While numerous life-extending manipulations have been discovered in the nematode Caenorhabditis elegans, one that remains most enigmatic is disruption of oxidative phosphorylation. In order to unravel how such an ostensibly deleterious manipulation can extend lifespan, we sought to identify the ensemble of nuclear transcription factors that are activated in response to defective mitochondrial electron transport chain (ETC) function. Using a feeding RNAi approach, we targeted over 400 transcription factors and identified 15 that, when reduced in function, reproducibly and differentially altered the development, stress response, and/or fecundity of isp-1(qm150) Mit mutants relative to wild-type animals. Seven of these transcription factors – AHA-1, CEH-18, HIF-1, JUN-1, NHR-27, NHR-49 and the CREB homolog-1 (CRH-1)-interacting protein TAF-4 – were also essential for isp-1 life extension. When we tested the involvement of these seven transcription factors in the life extension of two other Mit mutants, namely clk-1(qm30) and tpk-1(qm162), TAF-4 and HIF-1 were consistently required. Our findings suggest that the Mit phenotype is under the control of multiple transcriptional responses, and that TAF-4 and HIF-1 may be part of a general signaling axis that specifies Mit mutant life extension. PMID:24107417
Okumura, Yuuya; Nakamura, Tsuyoshi S; Tanaka, Takayuki; Inoue, Ichiro; Suda, Yasuyuki; Takahashi, Tetsuo; Nakanishi, Hideki; Nakamura, Shugo; Gao, Xiao-Dong; Tachikawa, Hiroyuki
Sporulation of Saccharomyces cerevisiae is a developmental process in which an ascus containing four haploid spores forms from a diploid cell. During this process, newly formed membrane structures called prospore membranes extend along the nuclear envelope and engulf and package daughter nuclei along with cytosol and organelles to form precursors of spores. Proteins involved in prospore membrane extension, Vps13 and Spo71, have recently been reported; however, the overall mechanism of membrane extension remains unclear. Here, we identified Spo73 as an additional factor involved in prospore membrane extension. Analysis of a spo73∆ mutant revealed that it shows defects similar to those of a spo71∆ mutant during prospore membrane formation. Spo73 localizes to the prospore membrane, and this localization is independent of Spo71 and Vps13. In contrast, a Spo73 protein carrying mutations in a surface basic patch mislocalizes to the cytoplasm and overexpression of Spo71 can partially rescue localization to the prospore membrane. Similar to spo71∆ mutants, spo73∆ mutants display genetic interactions with the mutations in the SMA2 and SPO1 genes involved in prospore membrane bending. Further, our bioinformatic analysis revealed that Spo73 is a dysferlin domain-only protein. Thus, these results suggest that a dysferlin domain-only protein, Spo73, functions with a dual pleckstrin homology domain protein, Spo71, in prospore membrane extension. Analysis of Spo73 will provide insights into the conserved function of dysferlin domains, which is related to dysferlinopathy. IMPORTANCE Prospore membrane formation consists of de novo double-membrane formation, which occurs during the developmental process of sporulation in Saccharomyces cerevisiae. Membranes are formed into their proper size and shape, and thus, prospore membrane formation has been studied as a general model of membrane formation. We identified SPO73, previously shown to be required for spore wall formation
Okumura, Yuuya; Nakamura, Tsuyoshi S.; Tanaka, Takayuki; Inoue, Ichiro; Suda, Yasuyuki; Takahashi, Tetsuo; Nakanishi, Hideki; Nakamura, Shugo; Gao, Xiao-Dong
ABSTRACT Sporulation of Saccharomyces cerevisiae is a developmental process in which an ascus containing four haploid spores forms from a diploid cell. During this process, newly formed membrane structures called prospore membranes extend along the nuclear envelope and engulf and package daughter nuclei along with cytosol and organelles to form precursors of spores. Proteins involved in prospore membrane extension, Vps13 and Spo71, have recently been reported; however, the overall mechanism of membrane extension remains unclear. Here, we identified Spo73 as an additional factor involved in prospore membrane extension. Analysis of a spo73∆ mutant revealed that it shows defects similar to those of a spo71∆ mutant during prospore membrane formation. Spo73 localizes to the prospore membrane, and this localization is independent of Spo71 and Vps13. In contrast, a Spo73 protein carrying mutations in a surface basic patch mislocalizes to the cytoplasm and overexpression of Spo71 can partially rescue localization to the prospore membrane. Similar to spo71∆ mutants, spo73∆ mutants display genetic interactions with the mutations in the SMA2 and SPO1 genes involved in prospore membrane bending. Further, our bioinformatic analysis revealed that Spo73 is a dysferlin domain-only protein. Thus, these results suggest that a dysferlin domain-only protein, Spo73, functions with a dual pleckstrin homology domain protein, Spo71, in prospore membrane extension. Analysis of Spo73 will provide insights into the conserved function of dysferlin domains, which is related to dysferlinopathy. IMPORTANCE Prospore membrane formation consists of de novo double-membrane formation, which occurs during the developmental process of sporulation in Saccharomyces cerevisiae. Membranes are formed into their proper size and shape, and thus, prospore membrane formation has been studied as a general model of membrane formation. We identified SPO73, previously shown to be required for spore wall
Anderson, Edward N; Corkins, Mark E; Li, Jia-Cheng; Singh, Komudi; Parsons, Sadé; Tucey, Tim M; Sorkaç, Altar; Huang, Huiyan; Dimitriadi, Maria; Sinclair, David A; Hart, Anne C
Moderate stress can increase lifespan by hormesis, a beneficial low-level induction of stress response pathways. 5'-fluorodeoxyuridine (FUdR) is commonly used to sterilize Caenorhabditis elegans in aging experiments. However, FUdR alters lifespan in some genotypes and induces resistance to thermal and proteotoxic stress. We report that hypertonic stress in combination with FUdR treatment or inhibition of the FUdR target thymidylate synthase, TYMS-1, extends C. elegans lifespan by up to 30%. By contrast, in the absence of FUdR, hypertonic stress decreases lifespan. Adaptation to hypertonic stress requires diminished Notch signaling and loss of Notch co-ligands leads to lifespan extension only in combination with FUdR. Either FUdR treatment or TYMS-1 loss induced resistance to acute hypertonic stress, anoxia, and thermal stress. FUdR treatment increased expression of DAF-16 FOXO and the osmolyte biosynthesis enzyme GPDH-1. FUdR-induced hypertonic stress resistance was partially dependent on sirtuins and base excision repair (BER) pathways, while FUdR-induced lifespan extension under hypertonic stress conditions requires DAF-16, BER, and sirtuin function. Combined, these results demonstrate that FUdR, through inhibition of TYMS-1, activates stress response pathways in somatic tissues to confer hormetic resistance to acute and chronic stress. C. elegans lifespan studies using FUdR may need re-interpretation in light of this work. PMID:26854551
40 CFR 62.14536 - What steps are required to request an extension of the initial compliance date if I plan to...
... extension of the initial compliance date if I plan to continue operation of my CISWI unit? 62.14536 Section... unit? If you plan to continue operation and want to come into compliance with the requirements of this... analyses undertaken to support your need for an extension, including an explanation of why you are...
Solbiati, José; Cronan, John E.
Transcription of the Escherichia coli biotin (bio) operon is regulated by BirA, a protein that is not only the repressor that regulates bio operon expression by DNA binding but also the enzyme that covalently attaches biotin to its cognate acceptor proteins. Binding of BirA to the bio operator requires dimerization of the protein that is triggered by BirA-catalyzed synthesis of biotinoyl-adenylate (bio-AMP), the obligatory intermediate of the attachment reaction. The current model postulates that the unmodified acceptor protein binds the monomeric BirA:bio-AMP complex and thereby blocks assembly (dimerization) of the form of BirA that binds DNA. We report that expression of fusion proteins that carry synthetic biotin accepting peptide sequences was as effective as the natural acceptor protein in derepression of bio operon transcription. These peptide sequences have sequences that are remarkably dissimilar to that of the natural acceptor protein and thus our data argue that the regulatory switch does not require the extensive protein-protein interactions postulated in the current model. PMID:20142036
Mariappan, Malaiyalam; Gande, Santosh Lakshmi; Radhakrishnan, Karthikeyan; Schmidt, Bernhard; Dierks, Thomas; von Figura, Kurt
Formylglycine-generating enzyme (FGE) catalyzes the oxidation of a specific cysteine residue in nascent sulfatase polypeptides to formylglycine (FGly). This FGly is part of the active site of all sulfatases and is required for their catalytic activity. Here we demonstrate that residues 34-68 constitute an N-terminal extension of the FGE catalytic core that is dispensable for in vitro enzymatic activity of FGE but is required for its in vivo activity in the endoplasmic reticulum (ER), i.e. for generation of FGly residues in nascent sulfatases. In addition, this extension is needed for the retention of FGE in the ER. Fusing a KDEL retention signal to the C terminus of FGE is sufficient to mediate retention of an N-terminally truncated FGE but not sufficient to restore its biological activity. Fusion of FGE residues 1-88 to secretory proteins resulted in ER retention of the fusion protein. Moreover, when fused to the paralog of FGE (pFGE), which itself lacks FGly-generating activity, the FGE extension (residues 34-88) of this hybrid construct led to partial restoration of the biological activity of co-expressed N-terminally truncated FGE. Within the FGE N-terminal extension cysteine 52 is critical for the biological activity. We postulate that this N-terminal region of FGE mediates the interaction with an ER component to be identified and that this interaction is required for both the generation of FGly residues in nascent sulfatase polypeptides and for retention of FGE in the ER. PMID:18305113
Manned geosynchronous mission requirements and system analysis study extension. Manned Orbital Transfer Vehicle (MOTV) capabilities handbook and user guide
The primary change in crew capsule definition is a smaller MOTV crew capsule, switching from a 3-man capsule to a 2-man capsule. A second change permitted crew accommodations for sleeping and privacy to be combined with the flight station. The current baseline DRM, ER1, requires 2 men for 3 to 4 days to repair a multi-disciplined GOE Platform and a modest amount of mission dedicated hardware. A 2-man MOTV crew capsule to be used as a design reference point for the OTV, and its interfaces between the STS and other associated equipment or facilities are described in detail. The functional capabilities of the 2-man capsule, as well as its application to a wide range of generic missions, is also presented. The MOTV turnaround is addressed and significant requirements for both space based and ground based scenarios are summarized.
McQuary, Philip R; Liao, Chen-Yu; Chang, Jessica T; Kumsta, Caroline; She, Xingyu; Davis, Andrew; Chu, Chu-Chiao; Gelino, Sara; Gomez-Amaro, Rafael L; Petrascheck, Michael; Brill, Laurence M; Ladiges, Warren C; Kennedy, Brian K; Hansen, Malene
Deficiency of S6 kinase (S6K) extends the lifespan of multiple species, but the underlying mechanisms are unclear. To discover potential effectors of S6K-mediated longevity, we performed a proteomics analysis of long-lived rsks-1/S6K C. elegans mutants compared to wild-type animals. We identified the arginine kinase ARGK-1 as the most significantly enriched protein in rsks-1/S6K mutants. ARGK-1 is an ortholog of mammalian creatine kinase, which maintains cellular ATP levels. We found that argk-1 is possibly a selective effector of rsks-1/S6K-mediated longevity and that overexpression of ARGK-1 extends C. elegans lifespan, in part by activating the energy sensor AAK-2/AMPK. argk-1 is also required for the reduced body size and increased stress resistance observed in rsks-1/S6K mutants. Finally, creatine kinase levels are increased in the brains of S6K1 knockout mice. Our study identifies ARGK-1 as a longevity effector in C. elegans with reduced RSKS-1/S6K levels. PMID:26923601
Agreement between Commonwealth of Pennsylvania and Association of Pennsylvania State College and University Faculties (APSCUF), July 1, 1981 to June 30, 1984 and Reopener and Contract Extension Agreement between Association of Pennsylvania State College and University Faculties and State System of Higher Education, July 1, 1983 to June 30, 1985.
Association of Pennsylvania State Coll. and Univ. Faculties, Harrisburg.
The collective bargaining agreement between the Commonwealth of Pennsylvania and the Association of Pennsylvania State College and University Faculties (APSCUF; 4,200 members) covering the period July 1, 1981 to June 30, 1984 is presented. Also included for this American Association of University Professors affiliated union is the reopener and…
Munkácsy, Erin; Khan, Maruf H; Lane, Rebecca K; Borror, Megan B; Park, Jae H; Bokov, Alex F; Fisher, Alfred L; Link, Christopher D; Rea, Shane L
Form 5-Mining venture agreement model form
This text acts as a reference of the basic terms and conditions for a negotiated mining venture agreement. Alternative clauses and provisions, along with extensive commentary, are supplied. The model form contains many articles which define and detail the process.
...The Commission is noticing a recently-filed Postal Service request to include a modified Norway Post Agreement within an existing competitive product. The modification includes an 18-month extension of the original agreement's term. This notice addresses procedural aspects of the...
Zaremba, Mindaugas; Toliusis, Paulius; Grigaitis, Rokas; Manakova, Elena; Silanskas, Arunas; Tamulaitiene, Giedre; Szczelkun, Mark D; Siksnys, Virginijus
The stress-sensitive restriction-modification (RM) system CglI from Corynebacterium glutamicum and the homologous NgoAVII RM system from Neisseria gonorrhoeae FA1090 are composed of three genes: a DNA methyltransferase (M.CglI and M.NgoAVII), a putative restriction endonuclease (R.CglI and R.NgoAVII, or R-proteins) and a predicted DEAD-family helicase/ATPase (N.CglI and N.NgoAVII or N-proteins). Here we report a biochemical characterization of the R- and N-proteins. Size-exclusion chromatography and SAXS experiments reveal that the isolated R.CglI, R.NgoAVII and N.CglI proteins form homodimers, while N.NgoAVII is a monomer in solution. Moreover, the R.CglI and N.CglI proteins assemble in a complex with R2N2 stoichiometry. Next, we show that N-proteins have ATPase activity that is dependent on double-stranded DNA and is stimulated by the R-proteins. Functional ATPase activity and extensive ATP hydrolysis (∼170 ATP/s/monomer) are required for site-specific DNA cleavage by R-proteins. We show that ATP-dependent DNA cleavage by R-proteins occurs at fixed positions (6-7 nucleotides) downstream of the asymmetric recognition sequence 5'-GCCGC-3'. Despite similarities to both Type I and II restriction endonucleases, the CglI and NgoAVII enzymes may employ a unique catalytic mechanism for DNA cleavage. PMID:25429977
20 CFR 439.620 - Cooperative agreement.
... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Cooperative agreement. 439.620 Section 439.620 Employees' Benefits SOCIAL SECURITY ADMINISTRATION GOVERNMENTWIDE REQUIREMENTS FOR DRUG-FREE WORKPLACE (FINANCIAL ASSISTANCE) Definitions § 439.620 Cooperative agreement. Cooperative agreement means...
46 CFR 390.5 - Agreement vessels.
... 46 Shipping 8 2011-10-01 2011-10-01 false Agreement vessels. 390.5 Section 390.5 Shipping MARITIME....5 Agreement vessels. (a) In general. 46 U.S.C. 53501 states the requirements for eligible, qualified and agreement vessels. The rules in this section further define such terms and state how vessels...
... 46 Shipping 8 2013-10-01 2013-10-01 false Agreement vessels. 390.5 Section 390.5 Shipping MARITIME....5 Agreement vessels. (a) In general. 46 U.S.C. 53501 states the requirements for eligible, qualified and agreement vessels. The rules in this section further define such terms and state how vessels...
... 46 Shipping 8 2014-10-01 2014-10-01 false Agreement vessels. 390.5 Section 390.5 Shipping MARITIME....5 Agreement vessels. (a) In general. 46 U.S.C. 53501 states the requirements for eligible, qualified and agreement vessels. The rules in this section further define such terms and state how vessels...
... 46 Shipping 8 2012-10-01 2012-10-01 false Agreement vessels. 390.5 Section 390.5 Shipping MARITIME....5 Agreement vessels. (a) In general. 46 U.S.C. 53501 states the requirements for eligible, qualified and agreement vessels. The rules in this section further define such terms and state how vessels...
Extension-twist coupling of composite circular tubes with application to tilt rotor blade design
Nixon, Mark W.
This investigation was conducted to determine if twist deformation required for the design of full-scale extension-twist-coupled tilt-rotor blades can be achieved within material design limit loads, and to demonstrate the accuracy of a coupled-beam analysis in predicting twist deformations. Two extension-twist-coupled tilt-rotor blade designs were developed based on theoretically optimum aerodynamic twist distributions. The designs indicated a twist rate requirement of between .216 and .333 deg/in. Agreement between axial tests and analytical predictions was within 10 percent at design limit loads. Agreement between the torsion tests and predictions was within 11 percent.
An Analysis of the Nature of Change in Staffing the Cooperative Extension Services with Special Reference to the Educational Requirements of College Graduates.
Deere, Runyan Elton
This study analyzed Cooperative Extension Service staffing changes and future needs and trends relative to generalist and specialist jobs, college majors, level of educational attainment, sources and availability of candidates, work experience, and starting salaries. A staffing model was developed as a frame of reference and as a means of data…
Jones, Alun R; Holdaway, George H; Steinmetz, Charles P
An equation is presented for calculating the heat flow required from the surface of an internally heated windshield in order to prevent the formation of ice accretions during flight in specified icing conditions. To ascertain the validity of the equation, comparison is made between calculated values of the heat required and measured values obtained for test windshields in actual flights in icing conditions. The test windshields were internally heated and provided data applicable to two common types of windshield configurations; namely the V-type and the type installed flush with the fuselage contours. These windshields were installed on a twin-engine cargo airplane and the icing flights were conducted over a large area of the United States during the winters of 1945-46 and 1946-47. In addition to the internally heated windshield investigation, some test data were obtained for a windshield ice-prevention system in which heated air was discharged into the windshield boundary layer. The general conclusions resulting from this investigation are as follows: 1) The amount of heat required for the prevention of ice accretions on both flush- and V-type windshields during flight in specified icing conditions can be calculated with a degree of accuracy suitable for design purposes. 2) A heat flow of 2000 to 2500 Btu per hour per square foot is required for complete and continuous protection of a V-type windshield in fight at speeds up to 300 miles per hour in a moderate cumulus icing condition. For the same degree of protection and the same speed range, a value of 1000 Btu per hour per square foot suffices in a moderate stratus icing condition. 3) A heat supply of 1000 Btu per hour per square foot is adequate for a flush windshield located well aft of the fuselage stagnation region, at speeds up to 300 miles per hour, for flight in both stratus and moderate cumulus icing conditions. 4) The external air discharge system of windshield thermal ice prevention is thermally
Kim, Sungjin; Selote, Devarshi S.; Vatamaniuk, Olena K.
Background Members of the HMT-1 (heavy metal tolerance factor 1) subfamily of the ATP-binding cassette (ABC) transporter superfamily detoxify heavy metals and have unique topology: they are half-molecule ABC transporters that, in addition to a single transmembrane domain (TMD1) and a single nucleotide-binding domain (NBD1), possess a hydrophobic NH2-terminal extension (NTE). These structural features distinguish HMTs from other ABC transporters in different species including Drosophila and humans. Functional ABC transporters, however, are comprised of at least four-domains (two TMDs and two NDBs) formed from either a single polypeptide or by the association of two or four separate subunits. Whether HMTs act as oligomers and what role the NTE domain plays in their function have not been determined. Methodology/Principal Findings In this study, we examined the oligomeric status of Caenorhabditis elegans HMT-1 and the functional significance of its NTE using gel-filtration chromatography in combination with the mating-based split-ubiquitin yeast two-hybrid system (mbSUS) and functional in vivo assays. We found that HMT-1 exists in a protein complex in C. elegans. Studies in S. cerevisiae showed that HMT-1 at a minimum homodimerizes and that oligomerization is essential for HMT-1 to confer cadmium tolerance. We also established that the NTE domain plays an important structural and functional role: it is essential for HMT-1 oligomerization and Cd-detoxification function. However, the NTE itself was not sufficient for oligomerization suggesting that multiple structural features of HMT-1 must associate to form a functional transporter. Conclusions The prominence of heavy metals as environmental toxins and the remarkable conservation of HMT-1 structural architecture and function in different species reinforce the value of continued studies of HMT-1 in model systems for identifying functional domains in HMT1 of humans. PMID:20886084
48 CFR 31.109 - Advance agreements.
...) Statistical sampling methods (see 31.201-6(c)(4). ... contractors should seek advance agreement on the treatment of special or unusual costs and on statistical sampling methodologies at 31.201-6(c). However, an advance agreement is not an absolute requirement and...
36 CFR 254.14 - Exchange agreement.
... required congressional oversight; (6) In the event of an appeal under 36 CFR part 215 or 36 CFR part 251... agreement. (c) In the event of a failure to perform or to comply with the terms of an exchange agreement..., title searches, title curative actions, cultural resource surveys and mitigation, hazardous...
Standards applicable to owners and operators of hazardous waste treatment, storage, and disposal facilities; liability coverage requirements--Environmental Protection Agency. Final rule and notice of extension of effective date.
The effective date for qualifications of insurers providing liability insurance used to satisfy liability coverage requirements applicable to owners or operators of hazardous waste management facilities, as such requirements are included in 40 CFR Parts 264 and 265, is extended from July 15, 1982, to October 16, 1982. The effective date for the rest of the liability coverage requirements remains July 15, 1982. This extension is being provided to allow 6 months between the date of promulgation and the effective date for the insurer qualification provision, in accordance with Section 3010(b) of the Resource Conservation and Recovery Act of 1976, as amended. During the period between July 15 and October 16, 1982, owners or operators may use certificates of insurance or policy endorsements that do not certify to the qualifications of the insurer. PMID:10256192