Source: http://www.google.de/patents/US6002137
Timestamp: 2013-06-18 22:57:34
Document Index: 93116006

Matched Legal Cases: ['art 10', 'art 11', 'art 10', 'art 10', 'art 10', 'art 10', 'art 10', 'art 11', 'art 10', 'art 10', 'art 10', 'art 10']

Patent US6002137 - Fluorescence detecting system - Google PatenteSuche Bilder Maps Play YouTube News Gmail Drive Mehr » Erweiterte Patentsuche | Webprotokoll | Anmelden Erweiterte Patentsuche PatenteA fluorescence detecting system detects auto fluorescence emitted from an intrinsic pigment in a part of an organism to be observed. An excitation light source projects onto the part to be observed excitation light in the wavelength range which can excite the intrinsic pigment of the organism to emit...http://www.google.de/patents/US6002137?utm_source=gb-gplus-sharePatent US6002137 - Fluorescence detecting system Ver�ffentlichungsnummerUS6002137 APublikationstypErteilung Anmeldenummer09/023,206 Ver�ffentlichungsdatum14. Dez. 1999Eingetragen13. Febr. 1998 Priorit�tsdatum13. Febr. 1997 Ver�ffentlichungsnummer023206, 09023206, US 6002137 A, US 6002137A, US-A-6002137, US6002137 A, US6002137A ErfinderKatsumi HayashiUrspr�nglich Bevollm�chtigterFuji Photo Film Co., Ltd.Patentzitate (2), Referenziert von (20), Klassifizierungen (21) Externe Links: USPTO, USPTO-Zuordnung, EspacenetFluorescence detecting systemUS 6002137 A Zusammenfassung A fluorescence detecting system detects auto fluorescence emitted from an intrinsic pigment in a part of an organism to be observed. An excitation light source projects onto the part to be observed excitation light in the wavelength range which can excite the intrinsic pigment of the organism to emit auto fluorescence. A first fluorescence detector extracts from the auto fluorescence emitted from the pigment a whole auto fluorescence component in a visible region having a predetermined wavelength range including a first relatively short wavelength range and a relatively long wavelength range. A second fluorescence detector extracts an auto fluorescence component in a second relatively short wavelength range in the visible region from the auto fluorescence. A divider carries out a division between the auto fluorescence components respectively extracted by the first and second fluorescence detector.
FIG. 3 is a view for illustrating the relation between the value of Ifλ.sub.1 (short wavelength component)/Ifλ.sub.2 (the whole visible auto fluorescence component) and a variable N/n=X obtained by standardizing the number of the fluorescent molecules which contribute to the short wavelength component by the number of the fluorescent molecules which contribute to the whole visible auto fluorescence component,
FIG. 4 is a view for illustrating the relation between the value of Ifλ.sub.1 (short wavelength component)/Ifλ.sub.2 (the sum fluorescence component) and a variable N/n=X obtained by standardizing the number of the fluorescent molecules which contribute to the short wavelength component by the number of the fluorescent molecules which contribute to the sum fluorescence component,
The excitation light L1 of a wavelength λ.sub.ex is emitted from the excitation light projecting means 1 and projected onto the diagnostic part 10 including a diseased part 11. Auto fluorescence L3 is emitted from the diagnostic part 10 by an intrinsic pigment and is divided into a short wavelength component and a whole visible auto fluorescence component by a dichroic mirror, an optical filter or the like. The first fluorescence detecting means 3 detects the short wavelength component of the auto fluorescence L3 and the second fluorescence detecting means 4 detects the whole visible self-wavelength component of the same. The first and second fluorescence detecting means 3 and 4 may be either a photodetector such as a photodiode which detects the auto fluorescence L3 from point to point or those such as a CCD image taking device which two-dimensionally detects the auto fluorescence L3 and forms a fluorescence image.
The apparent short wavelength component Ifλ.sub.1 :
If&#955;.sub.1 =k&#955;.sub.1  The apparent whole visible auto fluorescence component Ifλ.sub.2 :
If&#955;.sub.2 =k&#955;.sub.2  wherein
λ.sub.ex : the wavelength of the excitation light L1,
Iλ.sub.ex : the intensity of the excitation light L1 at the diagnostic part 10 which depends upon the distance L between the excitation light projecting means 1 and the diagnostic part 10, the power P of the excitation light projecting means 1 and the angle θ at which the light bundle of the excitation light L1 impinges upon the diagnostic part 10, Iλ.sub.ex =Iλ.sub.ex (L, P, θ),
kλ.sub.1 : a constant which depends upon the wavelength λ.sub.ex of the excitation light L1 and the apparent density N of the auto fluorescence molecules which contribute to the short wavelength component,
kλ.sub.2 : a constant which depends upon the wavelength λ.sub.ex of the excitation light L1 and the apparent density N of the auto fluorescence molecules which contribute to the whole visible auto fluorescence component,
ηFλ.sub.1 : the fluorescence quantum yield to the wavelength λ.sub.ex of the excitation light L1 of the auto fluorescence molecules which contribute to the short wavelength component,
ηFλ.sub.2 ; the fluorescence quantum yield to the wavelength λ.sub.ex of the excitation light L1 of the auto fluorescence molecules which contribute to the whole visible auto fluorescence component,
Then the divider means 5 divides the apparent short wavelength component Ifλ.sub.1 by the apparent whole visible auto fluorescence component Ifλ.sub.2. That is,
If&#955;.sub.1 /If&#955;.sub.2 =(k&#955;.sub.1  When
(k&#955;.sub.1  If&#955;.sub.1 /If&#955;.sub.2 =CX.
Since C is a constant, Ifλ.sub.1 /Ifλ.sub.2 can be plotted as shown in FIG. 3. That is, nonuniformity in the illuminance Iλ.sub.ex of the excitation light L1 from place to place can be cancelled. The value of X represents the number of the fluorescent molecules which contribute to the short wavelength component standardized by the number of the fluorescent molecules which contribute to the whole visible auto fluorescence component, and accordingly that Ifλ.sub.1 /Ifλ.sub.2 is small means that the part is diseased. Thus carrying out a division between the short wavelength component Ifλ.sub.1 and the whole visible auto fluorescence component Ifλ.sub.2, a diseased part can be specifically extracted. At this time by employing the whole visible auto fluorescence component Ifλ.sub.2 as a divisor, the divisor can be sufficiently large and the possibility of dividing a value by 0 can be avoided, whereby occurrence of operation error can be prevented. When image taking devices are employed as the first and second fluorescence detecting means 3 and 4, a fluorescence image whose fluorescence intensity is corrected can be displayed as a visible image by the display means 6.
The excitation light L1 of a wavelength λ.sub.ex is emitted from the excitation light projecting means 1 and projected onto the diagnostic part 10 including a diseased part 11. Auto fluorescence L3 is emitted from the diagnostic part 10 by an intrinsic pigment and is divided into a short wavelength component and a sum fluorescence component of an auto fluorescence component in a relatively short wavelength range in the visible region and an auto fluorescence component in a relatively long wavelength range in the visible region by a dichroic mirror, an optical filter or the like. The first fluorescence detecting means 3 detects the short wavelength component of the auto fluorescence L3 and the second fluorescence detecting means 4 detects the sum fluorescence component.
If&#955;.sub.1 =k&#955;.sub.1  The apparent long wavelength component Ifλ.sub.2 :
λ.sub.ex the wavelength of the excitation light L1,
Iλ.sup.ex : the intensity of the excitation light L1 at the diagnostic part 10 which depends upon the distance L between the excitation light projecting means 1 and the diagnostic part 10, the power P of the excitation light projecting means 1 and the angle θ at which the light bundle of the excitation light L1 impinges upon the diagnostic part 10, Iλ.sub.ex =Iλ.sub.ex (L, P, θ),
kλ.sub.2 : a constant which depends upon the wavelength λ.sub.ex of the excitation light L1 and the apparent density N of the auto fluorescence molecules which contribute to the long wavelength component,
ηFλ.sub.2 : the fluorescence quantum yield to the wavelength λ.sub.ex of the excitation light L1 of the auto fluorescence molecules which contribute to the long wavelength component,
Then the divider means 5 divides the short wavelength component by the sum fluorescence component (Ifλ.sub.1 +Ifλ.sub.2). That is,
If&#955;.sub.1 /(If&#955;.sub.1 +If&#955;.sub.2)=(k&#955;.sub.1   When
(k&#955;.sub.1   /(If&#955;.sub.1 +If&#955;.sub.2)=C
Since C is a constant, Ifλ.sub.1 /(Ifλ.sub.1 +Ifλ.sub.2) can be plotted as shown in FIG. 4. That is, nonuniformity in the illuminance Iλ.sub.ex of the excitation light L1 from place to place can be cancelled. The value of X represents the number of the fluorescent molecules which contribute to the short wavelength component standardized by the number of the fluorescent molecules which contribute to the long wavelength component, and accordingly that Ifλ.sub.1 /(Ifλ.sub.1 +Ifλ.sub.2) is small means that the part is diseased. Thus carrying out a division between the short wavelength component Ifλ.sub.1 and the sum fluorescence component (Ifλ.sub.1 +Ifλ.sub.2), a diseased part can be specifically extracted. At this time by employing the sum fluorescence component (Ifλ.sub.1 +Ifλ.sub.2) as a divisor, the divisor can be sufficiently large and the possibility of dividing a value by 0 can be avoided, whereby occurrence of operation error can be prevented. When image taking devices are employed as the first and second fluorescence detecting means 3 and 4, a fluorescence image whose fluorescence intensity is corrected can be displayed as a visible image by the display means 6.
The change-over mirror 121 is driven by the driver 123 under the control of a signal from the timing controller 158 and is moved to the position shown by the broken line not to interrupt the fluorescence L3. The fluorescence L3 passes by the mirror 121 and is focused on the cooled CCD camera 144 through a lens 141 and the switching optical filter 142. As described above, the switching optical filter 142 has the long pass filter 142a and the band pass filter 142b. As shown in FIG. 7A, the long pass filter 142a transmits the whole visible auto fluorescence component having wavelengths not shorter than 460 nm and as shown in FIG. 7B, the band pass filter 142b transmits a near-green fluorescence component having wavelengths in the range of 510.+-.10 nm.
Patentzitate Zitiertes PatentEingetragen Ver�ffentlichungsdatum Antragsteller TitelUS5647368 *28. Febr. 199615. Juli 1997Xillix Technologies Corp.Imaging system for detecting diseased tissue using native fluorsecence in the gastrointestinal and respiratory tractUS5833617 *6. M�rz 199710. Nov. 1998Fuji Photo Film Co., Ltd.Fluorescence detecting apparatus* Vom Pr�fer zitiert Referenziert von Zitiert von PatentEingetragen Ver�ffentlichungsdatum Antragsteller TitelUS649264629. Sept. 200010. Dez. 2002Fuji Photo Film Co., Ltd.Method of and apparatus for obtaining fluorescence imageUS651621710. Juli 20004. Febr. 2003Fuji Photo Film Co., Ltd.Fluorescence diagnosis systemUS682124513. Juli 200123. Nov. 2004Xillix Technologies CorporationCompact fluorescence endoscopy video systemUS6839375 *4. Sept. 20024. Jan. 2005Lambda Physik AgOn-line quality control of the key optical components in lithography lasers using laser induced fluorescenceUS689967515. Jan. 200231. Mai 2005Xillix Technologies Corp.Fluorescence endoscopy video systems with no moving parts in the cameraUS697049214. Mai 200329. Nov. 2005Lambda Physik AgDUV and VUV laser with on-line pulse energy monitorUS72838586. Juni 200116. Okt. 2007Fujifilm CorporationFluorescent-light image display method and apparatus thereforUS7341557 *26. Juli 200411. M�rz 2008Novadaq Technologies Inc.Compact fluorescence endoscopy video systemUS77225347. Jan. 200825. Mai 2010Novadaq Technologies, Inc.Compact fluorescence endoscopy video systemUS8019405 *4. Apr. 200213. Sept. 2011Richard Wolf GmbhDevice for the picture-providing diagnosis of tissue using one of at least two diagnosis modesUS828014021. Apr. 20112. Okt. 2012Cambridge Research & Instrumentation, Inc.Classifying image featuresUS838561523. Febr. 200926. Febr. 2013Cambridge Research & Instrumentation, Inc.Spectral imaging of biological samplesUS839196118. Sept. 20065. M�rz 2013Cambridge Research & Instrumentation, Inc.Spectral imagingCN1886089B23. Sept. 200410. Aug. 2011剑桥研究和仪器设备股份有限公司Spectral imaging of biological samplesEP1161919A2 *5. Juni 200112. Dez. 2001Fuji Photo Film Co., Ltd.Fluorescent-light image display method and apparatus thereforEP1392152A2 *1. M�rz 20023. M�rz 2004Anant AgrawalSystem and method for determining tissue characteristicsEP1681993A2 *23. Sept. 200426. Juli 2006Cambridge Research &amp; Instrumentation, Inc.Spectral imaging of biological samplesEP1731087A2 *13. Juli 200113. Dez. 2006Xillix Technologies Corp.Compact fluorescent endoscopy video systemEP1757223A2 *23. Sept. 200428. Febr. 2007Cambridge Research &amp; Instrumentation, Inc.Spectral imaging of biological samplesEP1759631A2 *5. Juni 20017. M�rz 2007Fuji Photo Film Co., LtdFluorescent-light image display method and apparatus therefor* Vom Pr�fer zitiertKlassifizierungen US-Klassifikation250/458.1, 600/478, 600/476Internationale KlassifikationG01N21/64, A61B5/00, A61B1/00, A61B1/04 UnternehmensklassifikationA61B1/043, A61B5/0084, A61B1/00009, A61B5/0071, A61B1/042, A61B1/00186, A61B1/0646, G01N21/6486 Europ�ische KlassifikationA61B5/00P12B, A61B1/04F, A61B1/00C1D, A61B5/00P5, A61B1/04D, G01N21/64RDrehenOriginalbildGoogle-Startseite - Sitemap - USPTO-Bulk-Downloads - Datenschutzerkl�rung - Nutzungsbedingungen - �ber Google Patente - Feedback gebenDaten bereitgestellt von IFI CLAIMS Patent Services.© 2012 Google