Source: http://www.google.com/patents/US5451241?dq=6,034,652
Timestamp: 2017-12-18 15:14:16
Document Index: 217325036

Matched Legal Cases: ['art 2', 'art 2', 'art 2', 'art 2', 'art 2', 'art 2', 'art 2', 'art 2', 'art 2', 'art 2']

Patent US5451241 - Oxygenated analogs of botanic seed - Google Patents
An analog of botanic seed is disclosed which comprises a plant embryo preferably encapsulated, or at least in contact with, a hydrated oxygenated gel. The gel can be oxygenated by passing oxygen gas through a gel solution before curing the gel or by exposing the gel to oxygen gas after curing. The gel...http://www.google.com/patents/US5451241?utm_source=gb-gplus-sharePatent US5451241 - Oxygenated analogs of botanic seed
Publication number US5451241 A
Application number US 08/069,542
Also published as US5236469, US5486218
Publication number 069542, 08069542, US 5451241 A, US 5451241A, US-A-5451241, US5451241 A, US5451241A
Inventors William C. Cartson, Jeffrey E. Hartle, Barbara K. Bower
Patent Citations (36), Non-Patent Citations (80), Referenced by (41), Classifications (10), Legal Events (5)
US 5451241 A
1. A method for manufacturing an analog of a botanic seed comprising:
preparing a liquid hydrated gel comprising a perfluorocarbon compound so as to enable the gel to acquire a concentration of molecular oxygen that is higher than a concentration of molecular oxygen that would otherwise be present in an otherwise similar gel, that lacks the perfluorocarbon compound, solely by absorption of oxygen from the atmosphere at standard temperature and pressure;
encapsulating a unit of totipotent plant tissue in the gel; and
2. A method for manufacturing an analog of a botanic seed as recited in claim 1 including the step of incorporating molecular oxygen in the gel by passing oxygen gas through the gel before curing the gel.
All Treatments were placed in covered Petri plates on the surface of nutrient agar medium (1% agar). Six embryos were placed in each plate and six replicate plates were prepared for each Treatment. All plates were placed in a 23° C. incubator under continuous filtered fluorescent light to stimulate germination. After 28 days, the plates were removed from the incubator and examined for quality and quantity of germinants.
All Treatments were placed in covered Petri plates on the surface of nutrient agar medium. Six embryos were placed in each plate and six replicate plates were prepared for each Treatment. All plates were placed in a 23° C. incubator under continuous filtered fluorescent light to stimulate germination. After 28 days, the plates were removed from the incubator and examined for quality and quantity of germinants. Results are tabulated in Table I.
To prepare agar blocks for Treatments (3) and (4), blocks of nutrient-containing agar were cut measuring about 4×4×5 mm using a small spatula. Using sterile forceps, an embryo was inserted into each block, centered in the block for Treatment (3) and with the radicle protruding outside the block for Treatment (4). Embryos were inserted into the blocks radicle-end first for Treatment (3) and cotyledon-end first for Treatment (4). With Treatment (4), about half the embryo length was left protruding from the agar block.
Bare embryos (Treatment (1)) and encapsulated embryos (Treatments (2)-(5)) were placed on nutrient-agar surfaces in Petri dishes. The dishes were covered and placed in a 23° C. incubator under continuous filtered fluorescent light for 35 days. Subsequent examination revealed the data shown in Table II.
Treatment (3): Glass shells having 5.2 mm inside diameter were made as described in Treatment (2) of Example 4. One end of each shell was sealed with an elastomeric septum, then the shells were sterilized. Each shell was then filled with nutrient agar. Small paper tubes having 2.5 mm inside diameter and about 5 mm long were made by cutting Whatman #1 qualitative filter paper into 5 mm-wide strips, each of which was rolled around a 2.5 mm outside diameter pin to form a paper tube. The tubes were kept from uncurling by application of a small piece of label tape (2×8 mm). The tubes were autoclaved and sealed on one end by dipping in hot paraffin. Each tube was axially inserted sealed-end first in an individual agar-containing glass shell until the open end of the tube was flush with the opening of the shell. An embryo was inserted in each paper tube cotyledon-end first until the radicle tip was flush with the tube opening.
Each Treatment involved six sets having six embryos per set. In Treatments (2)-(4), the resulting capsules were placed on their sides on nutrient agar surfaces in sterile covered Petri plates and incubated under continuous light for 35 days at 23° C. Data are tabulated in Table IV.
Treatment (2): A 1:1 v/v mixture of the 30% FC-77 emulsion with 2×-concentrated nutrient liquid containing alginate.
Treatment (3): A 2:1 v/v mixture of the 30% FC-77 emulsion and 3×-concentrated nutrient liquid containing alginate.
Treatment (4): A 3:1 v/v mixture of the 30% FC-77 emulsion and 4×-concentrated nutrient liquid containing alginate.
Treatment (5): A 4:1 v/v mixture of the 30% FC-77 emulsion and 5×-concentrated nutrient liquid containing alginate.
TABLE VI__________________________________________________________________________  % Normal        % That Grew                % Radicle                       % Hypocotyl                              % GerminationTreatment  Germinants        Thru Capsule                Germination                       Germination                              Hyp. & Rad.__________________________________________________________________________1 (Control)  92%   --      --     --     --2       7%   28%     17%    92%    17%3      17%   37%     45%    97%    45%4      46%   87%     87%    100%   87%5 (Control)  88%   --      --     --     --6       3%   24%     21%    100%   21%7       9%   24%     56%    94%    56%8      30%   55%     59%    92%    59%9 (Control)  92%   --      --     --     --10      9%   37%     40%    95%    40%11      3%   12%     15%    54%    15%12     32%   70%     71%    98%    71%13  (Control)  32%   --      --     --     --14      3%   28%     30%    100%   30%15     10%   34%     35%    100%   35%16     21%   42%     47%    100%   47%__________________________________________________________________________
TABLE VII__________________________________________________________________________ % Normal       % Growth              % Radicle                     % Hypocotyl                            % GerminationTreatment Germinants       Thru Capsule              Germination                     Germination                            Hyp. & Rad.__________________________________________________________________________1     56%   94%    94%    100%   94%2     70%   86%    86%     97%   86%3      0%    0%     0%     0%     0%4     15%   57%    59%    100%   59%5     24%   35%    41%     89%   35%6 (Control) 100%  --     --     --     --__________________________________________________________________________
TABLE VIII__________________________________________________________________________ % Normal       % Growth              % Radicle                     % Hypocotyl                            % GerminationTreatment Germinants       Thru Capsule              Germination                     Germination                            Hyp. & Rad.__________________________________________________________________________1     69%   92%    95%    100%   97%2     35%   70%    77%    100%   77%3     61%   81%    86%    100%   86%4     34%   56%    56%    100%   56%5     29%   60%    65%    100%   65%6 (Control) 97%   --     --     --     --__________________________________________________________________________
TABLE IX__________________________________________________________________________ % Normal       % Growth              % Radicle                     % Hypocotyl                            % GerminationTreatment Germinants       Thru Capsule              Germination                     Germination                            Hyp. & Rad.__________________________________________________________________________1     35%   82%    82%    100%   82%2     27%   52%    49%    100%   49%3     18%   36%    36%     97%   36%4      6%   19%    20%     95%   20%5      9%   28%    28%    100%   28%6      6%   34%    34%    100%   34%7 (Control) 94%   --     --     --     --__________________________________________________________________________
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U.S. Classification 47/57.6, 47/58.10R, 47/DIG.9
International Classification A01H4/00, A01C1/00, A01C1/06
Cooperative Classification Y10S47/09, Y10S47/11, A01H4/006