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US5187263A - Expression of biologically active PDGE analogs in eucaryotic cells - Google Patents
Expression of biologically active PDGE analogs in eucaryotic cells
US5187263A
US5187263A US07818397 US81839792A US5187263A US 5187263 A US5187263 A US 5187263A US 07818397 US07818397 US 07818397 US 81839792 A US81839792 A US 81839792A US 5187263 A US5187263 A US 5187263A
US07818397
EXAMPLE II Construction of the Plasmid pVSα
EXAMPLE III Construction of mllVS2α
EXAMPLE IV Construction of pVSBm
TABLE 1__________________________________________________________________________ZC299:    .sup.5' TAAG TGT GAA ATC GTT GCC GCG GCT AGA GCT GTT ACC    TAA TCT AGA.sup.3'ZC300:    .sup.3' GTACA TTC ACA CTT TAG CAA CGG CGC CGA TCT CGA CAA    TGG ATT AGA TCT GGCC.sup.5'__________________________________________________________________________
EXAMPLE V Yeast Transformation
EXAMPLE VI Construction of pSB1
EXAMPLE VII Construction of Variants and Derivatives of the B-chain
EXAMPLE VIII Construction of Variants and Derivatives of the A-chain
EXAMPLE IX Insertion of Expression Unit Constructions into pMPOT2
EXAMPLE X A-B Heterodimer Expression Construction
EXAMPLE XI Biological Activity Assays
TABLE 3______________________________________     PDGF mitogenic activity (ng/ml)Constructions       Media Concentrates                     Straight Media______________________________________pVSB        10,000 ng/ml  .sup.˜ 160 ng/mlpSB3         1,700 ng/mlpSB6          158 ng/mlpA5          1,025 ng/mlpA3                         50 ng/mlpA7                       .sup.˜ 150 ng/ml______________________________________
1. A mitogenic protein produced by a method comprising:
(a) introducing into a eucaryotic host cell a DNA construct capable of directing the expression and secretion of biologically active PDGF analogs in eucaryotic cells, said DNA construct containing a transcriptional promoter followed downstream by a DNA sequence that encodes the B-chain of a primate PDGF; and
(b) isolating from the host cell a mitogenic protein, which is a PDGF BB Romodimer, expressed from said DNA construct.
US07818397 1984-10-12 1992-01-02 Expression of biologically active PDGE analogs in eucaryotic cells Expired - Lifetime US5187263A (en)
US06896485 US4766073A (en) 1985-02-25 1986-08-13 Expression of biologically active PDGF analogs in eucaryotic cells
US23538188 true 1988-08-22 1988-08-22
US23538188 Continuation 1988-08-22 1988-08-22
US5187263A true US5187263A (en) 1993-02-16
ID=27540050
US07818397 Expired - Lifetime US5187263A (en) 1984-10-12 1992-01-02 Expression of biologically active PDGE analogs in eucaryotic cells
US (1) US5187263A (en)
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