Source: http://www.google.com/patents/US8131053?dq=6948823
Timestamp: 2017-08-19 04:33:58
Document Index: 578588685

Matched Legal Cases: ['§119', '§119', '§120', '§120', '§119', '§119']

Patent US8131053 - Detection of circulating tumor cells using imaging flow cytometry - Google Patents
Photometric and morphometric features derived from multi-mode imagery of cells in flow are used as a cell analyzer to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged. An imaging system simultaneously acquires a plurality of images...http://www.google.com/patents/US8131053?utm_source=gb-gplus-sharePatent US8131053 - Detection of circulating tumor cells using imaging flow cytometry
Publication number US8131053 B2
Application number US 12/181,062
Also published as US8548219, US20080317325, US20120148142
Publication number 12181062, 181062, US 8131053 B2, US 8131053B2, US-B2-8131053, US8131053 B2, US8131053B2
Inventors William Ortyn, David Basiji, Luchuan Liang, Vidya Venkatachalam, Philip Morrissey
Patent Citations (155), Non-Patent Citations (70), Referenced by (22), Classifications (17), Legal Events (2)
US 8131053 B2
This application is based on a prior copending provisional application, Ser. No. 60/952,522, filed on Jul. 27, 2007, the benefit of the filing date of which is hereby claimed under 35 U.S.C. §119(e). This application is a continuation-in-part of a copending patent application, Ser. No. 11/344,941, filed on Feb. 1, 2006, which itself is based on a prior provisional application, Ser. No. 60/649,373, filed on Feb. 1, 2005, the benefits of the filing dates of which are hereby claimed under 35 U.S.C. §119(e) and 35 U.S.C. §120.
Patent application Ser. No. 11/344,941 is also a continuation application based on a prior conventional application, Ser. No. 11/123,610, filed on May 4, 2005, which itself is based on a prior provisional application, Ser. No. 60/567,911, filed on May 4, 2004, and which is also a continuation-in-part of prior patent application Ser. No. 10/628,662, filed on Jul. 28, 2003, which issued as U.S. Pat. No. 6,975,400 on Dec. 13, 2005, which itself is a continuation-in-part application of prior patent application Ser. No. 09/976,257, filed on Oct. 12, 2001, which issued as U.S. Pat. No. 6,608,682 on Aug. 19, 2003, which itself is a continuation-in-part application of prior patent application Ser. No. 09/820,434, filed on Mar. 29, 2001, which issued as U.S. Pat. No. 6,473,176 on Oct. 29, 2002, which itself is a continuation-in-part application of prior patent application Ser. No. 09/538,604, filed on Mar. 29, 2000, which issued as U.S. Pat. No. 6,211,955 on Apr. 3, 2001, which itself is a continuation-in-part application of prior application patent application Ser. No. 09/490,478, filed on Jan. 24, 2000, which issued as U.S. Pat. No. 6,249,341 on Jun. 19, 2001, which itself is based on prior provisional patent application Ser. No. 60/117,203, filed on Jan. 25, 1999, the benefit of the filing dates of which is hereby claimed under 35 U.S.C. §120 and 35 U.S.C. §119(e). patent application Ser. No. 09/976,257, noted above, is also based on prior provisional application Ser. No. 60/240,125, filed on Oct. 12, 2000, the benefit of the filing date of which is hereby claimed under 35 U.S.C. §119(e).
Image Features (6 per object)
Background StdDev Intensity Standard deviation of intensity of pixels
Centmid X Intensity Intensity-weighted centroid of mask in
Centmid Y Intensity Intensity-weighted centroid of mask in
Gradient RMS RMS of intensity gradient of pixels within
Major Axis Intensity Intensity-weighted major axis of mask in
Mean Intensity Total Intensity of image divided by area of
Minor Axis Intensity Intensity-weighted minor axis of mask in
Object Rotation Angle Angle of major axis relative to axis of
Spot Large Total Sum of pixel intensities within large bright
Spot Medium Max Maximum pixel intensity within medium-
Spot Medium Total Sum of pixel intensities within medium-
Combined Mask Area Area of logical ‘OR’ of all six image
Flow Speed Camera line readout rate in Hertz at time
To investigate the usefulness of imaging flow cytometry for clinical FISH analysis, human peripheral blood mononuclear cells (PBMC) were obtained (AllCells, Emeryville, Calif.) and probed using a FISH in suspension (FISHIS) protocol developed at Amnis Corporation. The cells were fixed and permeabilized with successive incubations in 30% Camoy's solution in PBS (30 minutes at 4° C.), then 70% Carnoy's solution in PBS (10 minutes at 4° C.). After centrifugation, the cells were washed once in 2× saline sodium citrate (SSC), then resuspended in hybridization buffer containing the SpectrumGreen-labeled chromosome 12 enumeration probe according to the manufacturer's directions (Vysis, Des Plaines, Ill.). To hybridize the probe, cells in polymerase chain reaction tubes were exposed to 80° C. for 5 minutes and 42° C. for 2 hours in a DNA thermocycler. One hundred micro-liters of 2×SSC was added to the tubes and the cells pelleted by centrifugation. Cells were resuspended in 0.4×SSC containing 0.3% NP40 and exposed to 72° C. for 2 minutes. The cells were centrifuged and the pellets then resuspended in 50 micro-liters of 1% paraformaldehyde (in PBS). The sample was then loaded into the ImageStream™ system, and a file of 3500 cells was collected.
FIG. 15 is a gallery of 15 individual cells from the PBMC data file, numbered by the order in which they flowed through the instrument. Each cell is represented by a row of images (left to right): dark field, chromosome 12 fluorescence, bright field, and an overlay of the fluorescence and bright-field images. Doublets and larger clusters were eliminated from the analysis by plotting the area versus the aspect ratio of each cell's bright field image on a dot plot and gating on single cells, which represented approximately 60% of the data and were differentiated clearly as a population having an aspect (length-to-width) ratio close to one and lower area than doublets and clusters. No other pre-selection was performed, so the gallery represents an unbiased sampling of FISH data in PBMC populations. Most cells had two well-resolved FISH spots, corresponding to the two copies of chromosome 12. A fraction of the cells, however, had one or both FISH spots out of focus to some degree or only one apparent spot corresponding to a cell orientation that superimposed the FISH spots from the perspective of the imaging system. Defocus is a problem that increases with cell size, whereas the frequency of FISH spot superposition tends to decrease as cell size increases. The cells in the PBMC data file were found to have a mean diameter of 6.4±0.7 micrometers, which is small compared with the nuclear size of many epithelial cell types.
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US8548219 * Feb 14, 2012 Oct 1, 2013 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US20120148142 * Feb 14, 2012 Jun 14, 2012 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
International Classification G01J3/28, G06K9/00
Cooperative Classification G01N21/6458, G01N15/147, G01N2021/6441, G01N21/6428, G01N33/505, G01N15/1475, G01N33/5005
European Classification G01N15/14H1, G01N15/14H3, G01N21/64P4C, G01N33/50D, G01N33/50D2F2B, G01N21/64H
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:ORTYN, WILLIAM;BASIJI, DAVID;LIANG, LUCHUAN;AND OTHERS;REEL/FRAME:021504/0615