Source: http://www.google.com/patents/US6811968?ie=ISO-8859-1&dq=7,603,356
Timestamp: 2016-02-06 03:48:57
Document Index: 423393295

Matched Legal Cases: ['Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60', 'Application No. 60']

Patent US6811968 - Method of monitoring cell motility and chemotaxis - Google PatentsSearch Images Maps Play YouTube News Gmail Drive More »Sign inPatentsThe present invention discloses a method of monitoring chemotaxis or chemoinvasion comprising: providing a device for monitoring chemotaxis. The device includes a support member; a top member mounted to the support member by being placed in substantially fluid-tight, conformal contact with the support...http://www.google.com/patents/US6811968?utm_source=gb-gplus-sharePatent US6811968 - Method of monitoring cell motility and chemotaxisAdvanced Patent SearchPublication numberUS6811968 B2Publication typeGrantApplication numberUS 10/097,322Publication dateNov 2, 2004Filing dateMar 15, 2002Priority dateNov 8, 2000Fee statusLapsedAlso published asCA2429056A1, DE60136292D1, EP1339838A2, EP1339838A4, EP1339838B1, US6699665, US7166459, US7364899, US20020168757, US20030022197, US20030022269, WO2002048676A2, WO2002048676A3, WO2002048676A9Publication number097322, 10097322, US 6811968 B2, US 6811968B2, US-B2-6811968, US6811968 B2, US6811968B2InventorsGregory L. Kirk, Matthew Brown, Emanuele Ostuni, Enoch Kim, Bernardo D. Aumond, Olivier Schueller, Paul Sweetnam, Brian BenoitOriginal AssigneeSurface Logix Inc.Export CitationBiBTeX, EndNote, RefManPatent Citations (20), Non-Patent Citations (19), Referenced by (11), Classifications (52), Legal Events (8) External Links: USPTO, USPTO Assignment, EspacenetMethod of monitoring cell motility and chemotaxis
US 6811968 B2Abstract
This application is a continuation-in-part application of U.S. application Ser. No. 09/709,776, filed on Nov. 8, 2000, now U.S. Pat. No. 6,699,665, and claims the benefit of U.S. Provisional Application No. 60/307,886, filed on Jul. 27, 2001; U.S. Provisional Application No. 60/312,405, filed on Aug. 15, 2001; U.S. Provisional Application No. 60/323,742, filed on Sep. 21, 2001; U.S. Provisional Application No. 60/328,103, filed on Oct. 11, 2001; U.S. Provisional Application No. 60/330,456, filed on Oct. 22, 2001; U.S. Provisional Application No. 60/334,548, filed Dec. 3, 2001; and U.S. Provisional Application No. 60/363,355, filed on Mar. 12, 2002, all of which are herein incorporated by reference in their entirety.
The present invention also contemplates the patterning of more than one cell type on the upper surface of support member 16 constituting the bottom surface of well region 14 a in any one of the embodiments of the test device of the present invention, such as the embodiments shown in FIGS. 1A-14. Since cells of one type in vivo rarely exist in isolation and are instead in contact and communication with other cell types, it is desirable to have a system in which cells can be assayed in an environment more like that of the body. For example, since cancer cells are never found in isolation, but rather surrounded by normal cells, an assay designed to test the effect of a drug on cancer cells would be more accurate if the cancer cells in the assay were surrounded by normal cells. In testing an anti-cancer drug, cancer cells may be patterned on the upper surface of support member 16 constituting the bottom surface of well region 14 a in any given one of the embodiments of the test device of the present invention, such as the embodiments of FIGS. 1A-14, and then through a separate patterning procedure, the cancer cells may be surrounded by stromal cells. To pattern two different cell types on the upper surface of support member 16 constituting the bottom surface of well region 14 a, a micro cell position patterning member, as described above, is contacted with support member 16 and the outlined areas of the cell position patterning member are aligned with the portion of upper surface U of support member 16 that constitutes the bottom surface of well region 14 a, and will ultimately correspond to well region 14 a once top member 11 is contacted with support member 16. Cells of a first type may then be deposited over the cell position patterning member and filter through the micro through holes of the patterning member onto the portion of the upper surface U of support member 16 constituting the bottom surface of well region 14 a. The micro cell position patterning member may then be removed from support member 16. A macro cell position patterning member with outlined areas that correspond to the size and shape of wells 13 and 14 and may therefore correspond to the size and shape of the macrowells of a 96 well microtiter plate. The macro cell position patterning member has macro through holes. A macro through hole of the macro cell position patterning member encompasses an area larger than the surface area defined by a micro through hole of the micro cell position patterning member, but smaller than the surface area defined by well region 14 a of chamber 12. The macro cell position patterning member may then be contacted with support member 16. Cells of a second type may then be deposited over the macro cell position patterning member and filter through the macro through holes of the macro cell position patterning member onto the portion of upper surface U of support member 16 constituting the bottom surface of well regions 14 a once top member 11 is contacted with support member 16. Such patterning arrangement may result in cells of a second type surrounding and “stacking” cells of a first type. If it is desired to only have the cells of the second type stack the cells of the first type, then the same micro cell position patterning member used to deposit the first cell type or a different micro cell position patterning member having the exact same configuration as the patterning member used to deposit cells of a first type, may be used to deposit cells of a second type. After the cells are patterned on support member 16, top member 11 may be contacted with support member 16 such that through holes in top member 11 corresponding to the well region 14 a encompass the areas patterned with cells. This essentially results in cells being immobilized in a specific array within well region 14 a. Notwithstanding how many different cell types are patterned on the upper surface of support member 16 constituting the bottom surface of well region 14 a, the cells may be patterned on the support member through several methods known in the art. For example, the cells may be patterned on support member 16 through the use of SAMS. There are several techniques known in the art to pattern cells through the use of SAMs of which a few exemplary techniques disclosed in U.S. Pat. Nos. 5,512,131 to Kumar et al., 5,620,850 to Bambad et al., 5,721,131 to Rudolph et al., 5,776,748 and 5,976,826 to Singhvi et al. are incorporated by reference herein.
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Vollmer et al.: Journal of Leukocyte Biology (1992) vol. 52, No. 6, pp. 630-636.Referenced byCiting PatentFiling datePublication dateApplicantTitleUS7166459 *Mar 15, 2002Jan 23, 2007Surface Logix, Inc.Test device and method of making sameUS7314070 *Jan 18, 2005Jan 1, 2008President And Fellows Of Harvard CollegeMethod and apparatus for gradient generationUS7514256Feb 11, 2005Apr 7, 2009Emilio Barbera-GuillemBioreactor for selectively controlling the molecular diffusion between fluidsUS8790891 *Mar 19, 2010Jul 29, 2014The General Hospital CorporationMicrofluidic cell motility assayUS20020168757 *Mar 15, 2002Nov 14, 2002Gregory KirkTest device and method of making sameUS20050217750 *Jan 18, 2005Oct 6, 2005President And Fellows Of Harvard CollegeMethod and apparatus for gradient generationUS20060180529 *Feb 11, 2005Aug 17, 2006Emilio Barbera-GuillemBioreactor for selectively controlling the molecular diffusion between fluidsUS20110269226 *Aug 27, 2008Nov 3, 2011Agency For Science, Technology And ResearchMicrofluidic Continuous Flow Device for Culturing Biological MaterialUS20120003682 *Jan 5, 2012Gyros Patent AbMicrofabricated apparatus for cell based assaysUS20120094325 *Mar 19, 2010Apr 19, 2012The General Hosptial CorporationMicrofluidic Cell Motility AssayWO2006086552A2 *Feb 9, 2006Aug 17, 2006Emilio Barbera-GuillemBioreactor for selectively controlling the molecular diffusion between fluids* Cited by examinerClassifications U.S. Classification435/4, 435/5, 435/288.5, 435/7.23, 435/7.24International ClassificationG01N1/00, G01N37/00, C12Q1/68, B01L3/00, C12Q1/02, G01N35/02, C12N15/09, G01N15/14, G01N33/543, C40B40/10, G06T7/00, C40B60/14, C12M3/00, C40B40/06Cooperative ClassificationY10S435/81, B01J2219/00743, B01D2325/08, G06T7/0012, C40B40/10, C12Q1/025, G01N33/54366, G06T2207/30072, B01L2300/0636, B82Y30/00, C40B60/14, B01L2200/12, G06T7/004, B01J2219/00722, B01L3/5085, B01J2219/00317, B01L2300/0887, C40B40/06, B01L2300/0829, G01N2015/1497, B01L3/5027, B01J2219/00662, B01J2219/00725, B01L3/5025, B01L2200/0647, G01N15/1468European ClassificationB82Y30/00, B01L3/5085, C12Q1/02B, B01L3/5025, G06T7/00B2, G01N33/543K, G06T7/00PLegal EventsDateCodeEventDescriptionJun 14, 2002ASAssignmentOwner name: SURFACE LOGIX INC., MASSACHUSETTSFree format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:KIRK, GREGORY;BROWN, MATTHEW;OSTUNI, EMANUELE;AND OTHERS;REEL/FRAME:012997/0831;SIGNING DATES FROM 20020528 TO 20020607Oct 15, 2002ASAssignmentOwner name: SURFACE LOGIX, INC., MASSACHUSETTSFree format text: RE-RECORD TO CORRECT THE ASSIGNOR NAME PREVIOUSLY RECORDED AT REEL 012997/FRAME 0831. ASSIGNOR HEREBY CONFIRMS THE ASSIGNMENT OF THE ENTIRE INTEREST.;ASSIGNORS:KIRK, GREGORY L.;BROWN, MATTHEW;OSTUNI, EMANUELE;AND OTHERS;REEL/FRAME:013408/0844;SIGNING DATES FROM 20020528 TO 20020607Jun 12, 2008SULPSurcharge for late paymentJun 12, 2008FPAYFee paymentYear of fee payment: 4Nov 29, 2010ASAssignmentOwner name: SILICON VALLEY BANK, CALIFORNIAFree format text: SECURITY AGREEMENT;ASSIGNOR:SURFACE LOGIX, INC.;REEL/FRAME:025406/0642Effective date: 20101118Jun 18, 2012REMIMaintenance fee reminder mailedNov 2, 2012LAPSLapse for failure to pay maintenance feesDec 25, 2012FPExpired due to failure to pay maintenance feeEffective date: 20121102RotateOriginal ImageGoogle Home - Sitemap - USPTO Bulk Downloads - Privacy Policy - Terms of Service - About Google Patents - Send FeedbackData provided by IFI CLAIMS Patent Services