Patent Abstract:
the invention is the use of a therapeutically effective amount of glutathione in a liposome encapsulation for oral administration to improve symptoms of illnesses that are related to tuberculosis and hiv and more generally viruses and for the treatment and prevention of virus , particularly hhv - 6 and ebv , which liposomal encapsulation of glutathione is referred to as liposomal glutathione . the application references specifically reduced glutathione and its importance , and how to stabilize it effectively so it can be taken orally , and need not be refrigerated . new uses for tuberculosis are discussed . the combination is proposed of reduced glutathione and highly active anti - retroviral therapy having at least one pharmaceutical composition selected from the group of nucleoside / tide reverse transcriptase inhibitors , protease inhibitors , and non - nucleoside reverse transcriptase inhibitors , and further anti - tuberculosis drugs .

Detailed Description:
a lipid mixture having components lecithin , and glycerin were commingled in a large volume flask and set aside for compounding . in a separate beaker , a water mixture having water , glycerin , glutathione were mixed and heated to 50 . degree . c . the water mixture was added to the lipid mixture while vigorously mixing with a high speed , high shear homogenizing mixer at 750 - 1500 rpm for 30 minutes . the homogenizer was stopped and the solution was placed on a magnetic stirring plate , covered with parafilm and mixed with a magnetic stir bar until cooled to room temperature . normally , a spoilage retardant such as potassium sorbate or bht would be added . the solution would be placed in appropriate dispenser for ingestion as a liquid or administration as a spray . analysis of the preparation under an optical light microscope with polarized light at 400 × magnification confirmed presence of both multilamellar lipid vesicles ( mlv ) and unilamellar lipid vesicles . the preferred embodiment includes the variations of the amount of glutathione to create less concentrated amounts of glutathione . the methods of manufacture described in keller et al , u . s . pat . no . 5 , 891 , 465 , apr . 6 , 1999 , are incorporated into this description . ingredient concentration % sorbitan oleate 2 . 0 glutathione ( reduced ) 89 . 0 deionized water 4 . 0 potassium sorbate 0 . 2 polysorbate 20 2 . 0 phospholipon 90 ( dppc ) 2 . 0 components are commingled and liposomes are made using the injection method ( lasic , d ., liposomes , elsevier , 88 - 90 , 1993 ). when liposome mixture cooled down 0 . 7 ml was drawn into a 1 ml insulin syringe and injected into the open - end of a soft gelatin capsule then sealed with tweezers . large scale manufacturing methods for filling gel caps , such as the rotary die process , are the preferred method for commercial applications . the liposomal glutathione for this invention is and was made by biozone , inc . of pittsburg , calif . and sold by your energy systems , inc . of palo alto , calif . the preferred dosing schedule of the invention for the treatment of influenza symptoms is 600 mg ( 1 and ½ teaspoon ) of the invention to be taken at the first onset of symptoms . a dose of 400 mg ( 1 teaspoon ) to 600 mg is to be repeated each hour until symptoms are relieved . once symptom relief is achieved , the dose is repeated immediately upon the return of symptoms . the anticipated amount to be taken is 1 to 2 ounces in 24 hours . see case examples . if symptoms recur in the following 24 hours the regimen may be repeated as stated . 1 teaspoon of the invention of oral liposomal glutathione reduced contains approximately 440 mg gsh . a preferred mode sets a suggested dose based on body weight . recommended amounts are for use in the treatment of influenza symptoms . for best results it is suggested that the invention be used at the early onset of flu symptoms of as a preventative after exposure the flu . dose of liposomal reduced glutathione for individuals with hiv and / or tb is a range of ¼ teaspoon ( containing 100 mg reduced glutathione ) for every 30 pounds up to 100 pounds of weight . the dose over 100 pounds is a range of 1 . 0 teaspoon to 3 . 0 teaspoons twice a day . the preferred dose is 1 . 5 teaspoons twice a day orally in combination with the hiv drugs and / or the anti - tuberculosis drugs . dosing schedule for the treatment of acute and chronic symptoms of hhv - 6 virus such as chronic fatigue as stated , the initial dose should be according to body weight . for adults the dose is 1 and ½ teaspoon initially and repeat every 1 to 2 hours over 24 hour period . the amount and frequency of doses may be decreased as the individual begins to improve . the period of treatment will continue until severe symptoms are resolved . for chronic infections as seen with chronic fatigue syndrome , the present invention is continued at the level of 1 and ½ teaspoons twice a day until symptoms have abated . ingestion of the liposomal preparation of reduced glutathione can result in a rapid reduction in viral symptoms as related in the examples cited . the mechanism may be related to one or more of the methods described . the rapid addition of reduced glutathione to the system by the invention has a number of avenues to facilitate restoration of normal general cell and immune cell function that results in the reduction of symptoms related to hhv - 6 and virus infection in general . dosing schedule for the treatment of acute symptoms of hiv + virus and tuberculosis or tuberculosis alone or hhv - 6 virus or other virus such as encephalitis as stated , the initial dose should be according to body weight . for adults the dose is 1 and ½ teaspoon initially and repeat every 1 to 2 hours over 24 hour period . the amount and frequency of doses may be decreased as the individual begins to improve . the period of treatment will continue until severe symptoms are resolved . for chronic infections as seen with chronic fatigue syndrome , the present invention is continued at the level of 1 and ½ teaspoons twice a day until symptoms have abated . ingestion of the liposomal preparation of reduced glutathione can result in a rapid reduction in viral symptoms as related in the examples cited . the mechanism may be related to one or more of the methods described . the rapid addition of reduced glutathione to the system by the invention has a number of avenues to facilitate restoration of normal general cell and immune cell function that results in the reduction of symptoms related to hhv - 6 and virus infection in general . if the individual is not able to ingest oral medication the therapy is started with the intravenous infusion of glutathione in the following manner . the solution used for intravenous administration is prepared with glutathione concentrations of 200 mg per cc . the material is stored in vials of 10 cc for a total of 2000 mg per vial . the infusion may consist of 600 mg to 2000 mg given by rapid push infusion through an intravenous line . the infusion may be repeated on an hourly or as needed basis lessen the flu symptoms . providing the intravenous glutathione in a concentration that provides physiologic osmolarity is important . osmolarity is a measure of the osmotic pressure exerted by a solution across a perfect semi - permeable membrane . for instance , two identical solutions would have an osmolarity of zero . a solution that has twice as many particles on one side of a semi - permeable membrane as the other would have a higher osmolarity . the exact osmolarity of each solution would depend on the number of molecules or dissolved particles in the solution . in the body , we are looking at differences in the hundreds of milliosmoles , that is one - thousandth the concentration difference . osmolarity is dependent on the number of particles in solution , but independent of the nature of the particles . the following table provides concentrations of glutathione in sterile water to create normal or hypertonic osmolarity . the average osmolarity of human serum is 290 mosm . solutions in the range of 240 to 340 mosm are considered isotonic or roughly equivalent to the osmolarity of blood . solutions that are hypotonic relative to cells have fewer dissolved solids or solutes than the interior of surrounding cells and results in fluid being pulled into cells . thus , hypotonic fluids cause cells to swell and are considered dangerous to cells . strategies for formulating concentrations of the fluids for intravenous infusion that create isotonic or hypertonic solutions are more desirable than using hypotonic solutions . the infusion is continued at the rate of 2000 mg given over a period of 4 hours and repeated as needed on a continuous basis until the acute phase of the illness has resolved . after the individual is able to resume oral ingestion of medications the oral liposomal encapsulation of reduced glutathione form of the invention is initiated at a rate of 400 mg ., or one teaspoon every 2 hours . lower doses may be utilized over succeeding days until using the 1 and ½ teaspoon twice a day rate used for the long term therapy of non acute neurologic disease such as peripheral neuropathy described in the case example 2 . % w / w deionized water 71 . 9 glycerin 15 . 00 polysorbate - 20 2 . 50 lecithin 1 . 50 citrus seed extract 0 . 50 potassium sorbate 0 . 10 glutathione 8 . 50 ( reduced ) components lecithin , ethyl alcohol , cholesterol and glycerin were commingled in a large volume flask and set aside for compounding ( alternatively , in all of the embodiments where the glutathione ( reduced ) percentage is 8 . 5 , the glutathione ( reduced percentage ) can be lowered to 8 . 25 with 0 . 25 % tocopherol acetate added ). in that instance the table is ( example 5 ): % w / w deionized water 71 . 9 glycerin 15 . 00 polysorbate - 20 2 . 50 lecithin 1 . 50 citrus seed extract 0 . 50 potassium sorbate 0 . 10 glutathione 8 . 25 ( reduced ) in a separate beaker , water , hydroxy citric acid , glycerin , polysorbate 20 , glutathione were mixed and heated to 50 degrees c . the water mixture was added to the lipid mixture while vigorously mixing with a high speed , high shear homogenizing mixer at 750 - 1500 rpm for 30 minutes . the homogenizer was stopped and the solution was placed on a magnetic plate , covered with parafilm and mixed with a magnetic stir bar until cooled to room temperature . citrus seed extract were added and the solution was placed in appropriate dispenser for ingestion as a liquid or spray dispenser . analysis of the preparation under an optical light microscope with polarized light at 400 × magnification confirmed presence of both multilamellar lipid vesicles ( mlv ) and unilamellar lipid vesicles . the preferred embodiment includes the variations of the amount of glutathione to create less concentrated amounts of glutathione . the methods of manufacture described in keller et al , u . s . pat . no . 5 , 891 , 465 are incorporated into this description . a variation of the preferred embodiment of the invention is the addition of edta ( ethylene diamine tetraacetic acid ) 100 mg per ounce to be encapsulated in the liposome along with the glutathione . liposomal glutathione drink or spray 2500 mg per ounce or form suitable for encapsulation or gel % w / w deionized water 74 . 4 glycerin 15 . 00 lecithin 1 . 50 citrus seed extract 0 . 50 potassium sorbate 0 . 10 ( optional spoilage retardant ) glutathione 8 . 25 ( reduced ) a lipid mixture having components lecithin , ethyl alcohol and glycerin were commingled in a large volume flask and set aside for compounding . in a separate beaker , a water mixture having water , glycerin , glutathione were mixed and heated to 50 . degree . c . the water mixture was added to the lipid mixture while vigorously mixing with a high speed , high shear homogenizing mixer at 750 - 1500 rpm for 30 minutes . the homogenizer was stopped and the solution was placed on a magnetic stifling plate , covered with parafilm and mixed with a magnetic stir bar until cooled to room temperature . normally , citrus seed extract would be added . normally , a spoilage retardant such as potassium sorbate or bht would be added . the solution would be placed in appropriate dispenser for ingestion as a liquid or administration as a spray . however , in all examples given , and as shown in the table below , the formulation of liposomal glutathione can be formulated without the addition of potassium sorbate or citrus seed , or equivalent taste and / or preservative can be added so long as they do not impair the efficacy of the composition . analysis of the preparation under an optical light microscope with polarized light at 400 × magnification confirmed presence of both multilamellar lipid vesicles ( mlv ) and unilamellar lipid vesicles . the preferred embodiment includes the variations of the amount of glutathione to create less concentrated amounts of glutathione . the methods of manufacture described in keller et al u . s . pat . no . 5 , 891 , 465 are incorporated into this description . % w / w deionized water 73 . 55 glycerin 15 . 00 polysorbate - 20 2 . 50 lecithin 1 . 50 citrus seed extract 0 . 50 tocopherol acetate 0 . 25 potassium sorbate 0 . 10 glutathione ( reduced ) 3 . 30 edta 3 . 30 embodiment two of the invention includes the incorporation of the fluid liposome ( such as that prepared in example 1a ) into a gelatin based capsule to improve the stability , provide a convenient dosage form , and assist in sustained release characteristics of the liposome . the present embodiment relates to the use of glutathione in the reduced state encapsulated into liposomes or formulated as a preliposome formulation and then put into a capsule . the capsule can be a soft gel capsule capable of tolerating a certain amount of water , a two - piece capsule capable of tolerating a certain amount of water or a two - piece capsule where the liposomes are preformed then dehydrated . the liposome - capsule unit containing biologically encapsulated material can be taken in addition to orally , used for topical unit - of - use application , or other routes of application such as intra - occular , intranasal , rectal , or vaginal . the composition of examples 1 and 2 may be utilized in the encapsulated embodiment of this invention . gelatin capsules have a lower tolerance to water on their interior and exterior . the usual water tolerance for a soft gel capsule is 10 % on the interior . the concentration of water in a liposome formulation can range from 60 - 90 % water . an essential component of the present invention is the formulation of a liposome with a relatively small amount of water , in the range of 5 - 10 %. by making the liposome in a low aqueous system , the liposome is able to encapsulate the biologically active material and the exposure of water to the inside lining of the capsule is limited . the concentration of water should not exceed that of the tolerance of the capsule for which it is intended . the preferred capsule for this invention is one that can tolerate water in the 15 - 20 % range . the method described by keller et al , u . s . pat . no . 6 , 726 , 924 are incorporated in this description . components are commingled and liposomes are made using the injection method ( lasic , d ., liposomes , elsevier , 88 - 90 , 1993 ). when liposome mixture cooled down 0 . 7 ml was drawn into a 1 ml insulin syringe and injected into the open - end of a soft gelatin capsule then sealed with tweezers . the resulting capsule contains 10 mg coq10 . filling of gel caps on a large scale is best with the rotary die method or others such as the norton capsule machine . components are commingled and liposomes are made using the injection method ( lasic , d ., liposomes , elsevier , 88 - 90 , 1993 ). when liposome mixture cooled down 0 . 7 ml was drawn into a 1 ml insulin syringe and injected into the open - end of a soft gelatin capsule then sealed with tweezers . the resulting one gram capsule contains 898 iu of vitamin e . large scale manufacturing methods for filling gel caps , such as the rotary die process , are the preferred method for commercial applications . embodiment number three of the present invention includes the creation of liposome suspension using a self - forming , thermodynamically stable liposomes formed upon the adding of a diacylglycerol - peg lipid to an aqueous solution when the lipid has appropriate packing parameters and the adding occurs above the melting temperature of the lipid . the method described by keller et al , u . s . pat . no . 6 , 610 , 322 is incorporated into this description . most , if not all , known liposome suspensions are not thermodynamically stable . instead , the liposomes in known suspensions are kinetically trapped into higher energy states by the energy used in their formation . energy may be provided as heat , sonication , extrusion , or homogenization . since every high - energy state tries to lower its free energy , known liposome formulations experience problems with aggregation , fusion , sedimentation and leakage of liposome associated material . a thermodynamically stable liposome formulation which could avoid some of these problems is therefore desirable . the present embodiment prefers liposome suspensions which are thermodynamically stable at the temperature of formation . the formulation of such suspensions is achieved by employing a composition of lipids having several fundamental properties . first , the lipid composition must have packing parameters which allow the formation of liposomes . second , as part of the head group , the lipid should include polyethyleneglycol ( peg ) or any polymer of similar properties which sterically stabilizes the liposomes in suspension . third , the lipid must have a melting temperature which allows it to be in liquid form when mixed with an aqueous solution . by employing lipid compositions having the desired fundamental properties , little or no energy need be added when mixing the lipid and an aqueous solution to form liposomes . when mixed with water , the lipid molecules disperse and self assemble as the system settles into its natural low free energy state . depending on the lipids used , the lowest free energy state may include small unilamellar vesicle ( suv ) liposomes , multilamellar vesicle ( mlv ) liposomes , or a combination of suvs and mlvs . in one aspect , the invention includes a method of preparing liposomes . the method comprises providing an aqueous solution ; providing a lipid solution , where the solution has a packing parameter measurement of p a ( p a references the surface packing parameter ) between about 0 . 84 and 0 . 88 , a p v ( p v references the volume packing parameter ) between about 0 . 88 and 0 . 93 , ( see , d . d . lasic , liposomes , from physics to applications , elsevier , p . 51 1993 ), and where at least one lipid in the solution includes a polyethyleneglycol ( peg ) chain ; and combining the lipid solution and the aqueous solution . the peg chain preferably has a molecular weight between about 300 daltons and 5000 daltons . kinetic energy , such as shaking or vortexing , may be provided to the lipid solution and the aqueous solution . the lipid solution may comprise a single lipid . the lipid may comprise dioleolylglycerol - peg - 12 , either alone or as one of the lipids in a mixture . the method may further comprise providing an active compound , in this case glutathione ( reduced ); and combining the active compound with the lipid solution and the aqueous solution . initially , for patient treatment , reference the recommended or suggested doses on the drug package insert for anti - retroviral treatment and administer that amount . for persons sensitive to the arv drugs , due to the synergies between liposomal glutathione and arv drugs , on a physician &# 39 ; s recommendation , it may be possible to maintain the suggested liposomal glutathione doses above , but reduce the dose of the arv drug to 70 % of the recommended dose , 80 % of the recommended dose , or 90 % of the recommended dose . this reduced dosage would avoid some side effects resulting from many of the arv drugs , including dyslipidemia . simultaneously , 3 . 3 %, 4 %, 5 %, 6 %, 7 %, 7 . 5 %, 8 %, 8 . 5 % or 9 % lipoceutical glutathione may be used with anti - tuberculosis drugs . initially , reference the recommended or suggested doses on the drug package insert for anti - tuberculosis treatment . for persons sensitive to the anti - tuberculosis drugs , due to the synergies between liposomal glutathione and anti - tuberculosis drugs , on a physician &# 39 ; s recommendation , it may be possible to maintain the suggested liposomal glutathione doses above , but reduce the dose of the anti - tuberculosis drug to 70 % of the recommended dose , 80 % of the recommended dose , or 90 % of the recommended dose . with respect to combinations and methods with arv drugs , the liposomal glutathione should preferably be at 8 . 0 , 8 . 25 , 8 . 5 or 9 % w / w to maintain the synergy between the pharmaceutical compositions . chris t is a 37 year old man who presents with fatigue , weakness , diaphoresis , pallor and a sense of exhaustion . the symptoms had been present and progressing over a 14 day period of time , following an episode described as a “ bad flu ”. at the time of evaluation at 10 am he was considering returning to bed as even light lifting tasks and standing as part of his sales job was exhausting . 600 mg of oral liposomal glutathione was administered and the individual observed . he noted that approximately 45 minutes after ingesting the invention his symptoms began to lessen . his color returned to normal , the diaphoresis ceased and he felt a significant return of energy and strength . the improvement lasted almost an hour when his symptoms began to return . chris t . repeated the 600 mg dose and 20 to 30 minutes later again felt resolution of his symptoms . he repeated this schedule every 1 to 2 hours through the day . by 8 pm he had ingested 1 and ½ ounces ( approximately 3750 mg ) of the invention and his symptoms had resolved completely . using the invention through the day , he was able to complete his sales job , which on that day included standing all day , some light lifting of his product and interacting with customers continually through the day . the next morning in this example 2 , chris t ., reported that his flu symptoms had abated . l . m . is a 79 year old woman with a history peripheral neuropathy affecting her legs that has been present for 10 years . the patient &# 39 ; s neuropathy has prevented her from standing on hard surfaces due to the pain that activity induced . she used a wheelchair for shopping and was not able to stand on the hard ceramic tiles of her kitchen . l . m . initiated use of the invention in the form of oral liposomal glutathione at the rate of 1 and ½ teaspoons per day . she was using no other medications . after use of the invention for 8 weeks she began to notice a decrease in the pain . at 10 weeks she reported that she could again stand on her kitchen floor for the two hours that it required to cook a dinner . hhv - 6a is a cell associated virus ; cell free virus is often not very infectious . therefore , an assay was used that combined hhv - 6 infected cells with uninfected cells . a study by a laboratory independent of the inventor commissioned by a group , the hhv - 6 foundation , which is also independent from the inventor were run . to various cultures of this mixture of cells , the submitted drugs , at various concentrations were added . the positive control was the cell combination with no drug and the negative control was uninfected cells only . after the assay was allowed to run for 7 days , a fluorometric cytoproliferation assay was run and all assay conditions were calculated as a percentage of the negative control . if a drug assay was at least 90 % of the negative control , it was scored as being effective against hhv - 6 . a parallel cytotoxicity assay was run without infected cells to test whether the drugs are cytotoxic to the hsb 2 cells used in this experiment . hhv - 6a gs . human herpesvirus 6a , strain gs is adapted for growth in tissue culture . hhv - 6a is the strain most commonly reactivated in aids patients and in patients with multiple sclerosis . hsb - 2 , a human t - lymphoblastoid suspension cell line , was derived from the peripheral blood buffy coat of a patient with acute lymphoblastic leukemia and propagated as tumors in newborn syrian hamsters . positive control — cultures of infected and uninfected cells at a ratio of one infected cell for every four uninfected cells , no drugs or experimental reagents . cytotoxicity controls — drugs run at same concentrations as for the antiviral assay , but with uninfected cells only . drug comparison control : one plate was run with foscarnet , ganciclovir , and cidofovir . in addition a foscarnet comparison was run on each test drug plate . 200 μl cultures , plated with 5 × 103 uninfected cells per culture plus 1 . 25 × 103 infected cells , if infected cells are present in the culture . four replicates were run for each foscarnet , ganciclovir and cidofovir concentration on the comparison control plates ( antiviral and cytotoxicity ). in addition on each antiviral experimental drug plate there were duplicate wells of each foscarnet concentration . 10 replicates were run at each concentration of each experimental drug in the antiviral assay and for the cytotoxicity controls 4 replicates were run for the experimental drugs . cells were allowed to grow for seven days at which time the experiment was terminated and the cytoproliferation assay was run . read on a fluorometric reader at excitation of 530 nm , emission of 580 nm , and a gain of 35 . the average of the negative control is set at 100 %, and the average of the other assay conditions are represented as a percentage of the negative control . this study is considered valid when the positive control shows evidence of viral infection ( cytopathological effect ) and is 65 % or less of the negative control . the negative control should appear as a healthy growing culture by microscopic inspection . the final report contains the fluorometric readings for each culture , the average of replicate cultures , and each assay condition is presented as a percentage of the negative control . these data are presented in tabular form in an appendix . criteria for cytotoxicity : if the average of the cultures with drug but without virus is 85 % of the negative control that concentration of drug is judged as not being cytotoxic . if the percentage is between 75 % and 85 % of the negative control is said to have slight cytotoxicity . any value below 75 % is scored as cytotoxic . criteria for antiviral efficacy : if the average growth for infected cultures at a specific drug concentration is over 90 % of the negative control , the drug is scored as effective against hhv - 6a . if the average is between 90 % and 10 % above the average for the positive control the drug at that concentration is scored as partially effective against the virus . if it is 5 %- 10 % above the positive control it is scored as slightly effective . scores within 5 % of the positive control are judged as ineffective against the virus . scores below 5 % of the positive control are judged as being due to the cytotoxicity of the drug . study 1 : in an initial study several drugs known to be effective against hhv - 6 were evaluated for their efficacy . the material used included foscarnet ( phosphonoformic acid ) sigma p6801 , ganciclovir , sigma g2536 , cidofovir ( vistide injection 75 mg / ml ) gilead sci , amantadine , sigma a1260 , ribavirin , sigma r9644 , doxycyline hyclate , sigma d9891 , pbs 119 , ( combination of chloroquine , verapamil , dilantin and quercetin ), chloroquine diphosphate , sigma c6628 , neem elixir , glycyrrhizic acid , sigma g2135 , and lithium carbonate , sigma l4283 . study 1 summary : various drugs were tested in vitro to see if they suppress the propagation of hhv - 6a gs into uninfected hsb - 2 cells . cultures with infected and uninfected cells were given various dosages of the drugs being tested and allowed to grow for 7 days . at the end of seven day a fluorometric cytoproliferation assay was preformed and the growth of uninfected cells ( negative control ) was compared to the growth of infected cells without drug ( positive control ) and the growth of the cells with the various drugs . cytotoxicity controls were run with only uninfected cells and the drugs . no drug tested was able to suppress hhv - 6 completely or better able to suppress viral propagation than foscarnet . experimental drugs : nexavir ( kutapressin ), l - lysine , sigma l9037 , gabapentin ( neurotonin ), sigma g154 , lovenex ( heparin , enoxaparin sodium ) compound x from company x , oleuropein ( olive leaf extract ), immunopro , ( non - denatured whey protein ), lactoferrin from bovine milk , sigma l9507 , compound x ( company x substance a000556500 ), lipoceutical glutathione ™ ( readisorb products , your energy systems , inc ., 555 bryant st ., # 305 , palo alto , calif . 94301 ), resveratrol , sigma r5010 , fw 228 . 2 . percentage increase over positive ( infected ) control at optimal dosage : foscarnet 20 %. lipoceutical glutathione ™ 30 %. additional testing is being performed to determine optimal effective range and no cytotoxicity was found for lipoceutical glutathione ™. conclusion : of the twenty compounds tested , lipoceutical glutathione ™, the trade name of the present liposomal glutathione invention , showed efficacy against hhv - 6 virus . the study also demonstrated that there was no cytotoxicity from liposomal formulated reduced glutathione . e . w . a 48 year old woman who has experienced severe fatigue symptoms for over 20 years . she relates that while her symptoms developed after exposure to paints and solvent exposure , there was no clear toxin identified and a chronic viral component has been suspected . recently , the symptom complex had expanded to include loose stools that had been present for one month . e . w . started liposomal glutathione at 1 and ½ teaspoons once a day in 3 divided doses . after a week of use she noted that her stools had become more firm . after a month of use , her stools became normal . at the time of this report , the individual had been using the liposomal glutathione for 3 months . she noted that she had more stamina , although she was not yet able to return to work . at the same time she was able to tolerate emotional stresses that normally would have caused a significant setback and prolonged exhaustion . e . w . reports that since using the liposomal glutathione she is functioning significantly better than she has since developing the chronic fatigue symptoms . 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