Patent Publication Number: US-2017348324-A1

Title: Derivatives of betulin

Description:
FIELD OF THE INVENTION 
     The present invention relates to compounds, pharmaceutical compositions, and methods of use thereof for (i) inhibiting HIV replication in a subject infected with HIV, or (ii) treating a subject infected with HIV, by administering such compounds. 
     BACKGROUND OF THE INVENTION 
     Human immunodeficiency virus type 1 (HIV-1) leads to the contraction of acquired immune deficiency disease (AIDS). The number of cases of HIV continues to rise, and currently over twenty-five million individuals worldwide suffer from the virus. Presently, long-term suppression of viral replication with antiretroviral drugs is the only option for treating HIV-1 infection. Indeed, the U.S. Food and Drug Administration has approved twenty-five drugs over six different inhibitor classes, which have been shown to greatly increase patient survival and quality of life. However, additional therapies are still required because of undesirable drug-drug interactions; drug-food interactions; non-adherence to therapy; and drug resistance due to mutation of the enzyme target. 
     Currently, almost all HIV positive patients are treated with therapeutic regimens of antiretroviral drug combinations termed, highly active antiretroviral therapy (“HAART”). However, HAART therapies are often complex because a combination of different drugs must be administered often daily to the patient to avoid the rapid emergence of drug-resistant HIV-1 variants. Despite the positive impact of HAART on patient survival, drug resistance can still occur. The emergence of multidrug-resistant HIV-1 isolates has serious clinical consequences and must be suppressed with a new drug regimen, known as salvage therapy. 
     Current guidelines recommend that salvage therapy includes at least two, and preferably three, fully active drugs. Typically, first-line therapies combine three to four drugs targeting the viral enzymes reverse transcriptase and protease. One option for salvage therapy is to administer different combinations of drugs from the same mechanistic class that remain active against the resistant isolates. However, the options for this approach are often limited, as resistant mutations frequently confer broad cross-resistance to different drugs in the same class. Alternative therapeutic strategies have recently become available with the development of fusion, entry, and integrase inhibitors. However, resistance to all three new drug classes has already been reported both in the lab and in patients. Sustained successful treatment of HIV-1-infected patients with antiretroviral drugs will therefore require the continued development of new and improved drugs with new targets and mechanisms of action. 
     Presently, long-term suppression of viral replication with antiretroviral drugs is the only option for treating HIV-1 infection. To date, a number of approved drugs have been shown to greatly increase patient survival. However, therapeutic regimens known as highly active antiretroviral therapy (HAART) are often complex because a combination of different drugs must be administered to the patient to avoid the rapid emergence of drug-resistant HIV-1 variants. Despite the positive impact of HAART on patient survival, drug resistance can still occur. 
     The HIV Gag polyprotein precursor (Pr55Gag), which is composed of four protein domains—matrix (MA), capsid (CA), nucleocapsid (NC) and p6—and two spacer peptides, SP1 and SP2, represents a new therapeutic target. Although the cleavage of the Gag polyprotein plays a central role in the progression of infectious virus particle production, to date, no antiretroviral drug has been approved for this mechanism. 
     In most cell types, assembly occurs at the plasma membrane, and the MA domain of Gag mediates membrane binding. Assembly is completed by budding of the immature particle from the cell. Concomitant with particle release, the virally encoded PR cleaves Gag into the four mature protein domains, MA, CA, NC and p6, and the two spacer peptides, SP1 and SP2. Gag-Pol is also cleaved by PR, liberating the viral enzymes PR, RT and IN. Gag proteolytic processing induces a morphological rearrangement within the particle, known as maturation. Maturation converts the immature, donut-shaped particle to the mature virion, which contains a condensed conical core composed of a CA shell surrounding the viral RNA genome in a complex with NC and the viral enzymes RT and IN. Maturation prepares the virus for infection of a new cell and is absolutely essential for particle infectivity. 
     Bevirimat (PA-457) is a maturation inhibitor that inhibits the final step in the processing of Gag, the conversion of capsid-SP1 (p25) to capsid, which is required for the formation of infectious viral particles. Bevirimat has activity against ART-resistant and wild-type HIV, and has shown synergy with antiretrovirals from all classes. Bevirimat reduced HIV viral load by a mean of 1.3 log 10 /mL in patients who achieved trough levels of &gt;=20 μg/mL and who did not have any of the key baseline Gag polymorphisms at Q369, V370 or T371. However, Bevirimat users with Gag polymorphisms at Q369, V370 or T371 demonstrated significantly lower load reductions than patients without Gag polymorphisms at these sites. 
     Other examples of maturation inhibitors can be found in PCT Patent Application No. WO2011/100308, “Derivatives of Betulin”; PCT Patent Application No. PCT/US2012/024288, “Novel Anti-HIV Compounds and Methods of Use Thereof”; Chinese PCT Application No. PCT/CN2011/001302, “Carbonyl Derivatives of Betulin”; Chinese PCT Application No. PCT/CN2011/001303, “Methylene Derivatives of Betulin”; Chinese PCT Application Nos. PCT/CN2011/002105 and PCT/CN2011/002159, “Propenoate Derivatives of Betulin”. Maturation inhibitors in the prior art leave open gaps in the areas of polymorphism coverage whereby potency against a broad range of clinically relevant gag sequences is extremely important, along with overall potency including the clinically relevant protein adjusted antiviral activity that will be required for robust efficacy in long term durability trials. To date, no maturation inhibitor has achieved an optimal balance of these properties. 
     It would therefore be an advance in the art to discover alternative compounds that are an effective balance of the aforementioned properties for the prevention and/or treatment of HIV infections. 
     SUMMARY OF THE INVENTION 
     In accordance with one embodiment of the present invention, there is provided a compound of Formula I: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are independently selected from a bond or [C(R 6 R 6′ )] q ; 
     each instance of Q is independently selected from —CH 2 — or —C(═O)—; 
     W is selected from a bond or O; 
     R 1  is selected from the group consisting of hydrogen, (C 1 -C 12 )alkyl, —C(O)R 5 , —CH 2 &#39;O—(C 1 -C 6 )alkyl, 2-tetrahydro-2H-pyran, and 
     
       
         
         
             
             
         
       
     
     R 2  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —(C 1 -C 6 )alkyl-OR 4 , —(C 1 -C 6 )alkyl-O—(C 1 -C 6 )alkyl, —C(O)R 5 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r N + (R 4 ) 3 , wherein when W is O, R 1  and R 2  can optionally be taken together with the O and N to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring, wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 3  is selected from the group consisting of hydrogen, (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 2  and R 3  can optionally be taken together with the nitrogen and L 2  to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring, wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —R 3 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 . 
     R 6  and R 6′  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —Y, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 , wherein the R 6  and R 6′  groups can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring, and wherein the cycloalkyl ring may be optionally substituted by one to three R 11  groups; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, -Q-aryl-(R 4 ) n , —NR 14 R 15 , —C(O)CH 3 , wherein R 7  and R 8  can optionally be taken together with the nitrogen to which they are joined to form a 4 to 8 membered heterocyclyl or heteroaryl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 11  groups; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , —CO(O)R 4 , and —CO(O)R 5 , wherein any two R 11 , R 12  or R 13  groups can optionally join to form a 3 to 8 membered cycloalkyl, aryl, heterocyclyl or heteroaryl ring, wherein the heterocyclyl or heteroaryl ring may contain one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, and wherein the cycloalkyl, aryl, heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 16  groups; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 , wherein R 14  and R 15  can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring or 4 to 8 membered heterocyclyl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the cycloalkyl ring or heterocyclyl ring may be optionally substituted by one to three R 16  groups; 
     R 16  is independently selected from the group consisting of —H, halo, oxo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocyclyl, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, 2, 3, or 4; 
     p is independently 0, 1, 2, 3, or 4; and 
     r and q in each instance are independently 0, 1, 2, 3, or 4. 
     In a second aspect, the present invention relates to a pharmaceutical composition comprising a) the compound of Formula I or Formula II or a pharmaceutically acceptable salt the thereof; and b) a pharmaceutically acceptable excipient. 
     In a third aspect, the present invention is a method of treating an HIV infection comprising administering to a subject suffering therefrom a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof. 
     Compounds of the present invention are useful for the treatment of subjects with an HIV infection or for the treatment of subjects at risk of acquiring an HIV infection. 
    
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         FIG. 1  shows a bar graph representing a comparison between Bevirimat and compound 51 of their relative ability to inhibit HIV reverse transcriptase activity across a broad panel of HIV-1 isolates. 
         FIG. 2  shows a bar graph representing a comparison between compound 51 and compound C of their relative ability to inhibit HIV reverse transcriptase activity across a broad panel of HIV-1 isolates. 
         FIG. 3  shows a line graph representing an extrapolation of Bevirimat normalized IC 50  values to 100% human serum. 
         FIG. 4  shows a line graph representing an extrapolation of compound B normalized IC 50  values to 100% human serum. 
         FIG. 5  shows a line graph representing an extrapolation of compound 51 normalized IC 50  values to 100% human serum. 
     
    
    
     DETAILED DESCRIPTION OF REPRESENTATIVE EMBODIMENTS 
     Throughout this application, references are made to various embodiments relating to compounds, compositions, and methods. The various embodiments described are meant to provide a variety of illustrative examples and should not be construed as descriptions of alternative species. Rather it should be noted that the descriptions of various embodiments provided herein may be of overlapping scope. The embodiments discussed herein are merely illustrative and are not meant to limit the scope of the present invention. 
     It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention. In this specification and in the claims that follow, reference will be made to a number of terms that shall be defined to have the following meanings. 
     As used herein unless otherwise specified, “alkyl” refers to to a monovalent saturated aliphatic hydrocarbyl group having from 1 to 14 carbon atoms and, in some embodiments, from 1 to 6 carbon atoms. “(C x -C y )alkyl” refers to alkyl groups having from x to y carbon atoms. The term “alkyl”includes, by way of example, linear and branched hydrocarbyl groups such as methyl (CH 3 —), ethyl (CH 3 CH 2 —), n-propyl (CH 3 CH 2 CH 2 —), isopropyl ((CH 3 ) 2 CH—), n-butyl (CH 3 CH 2 CH 2 CH 2 —), isobutyl ((CH 3 ) 2 CHCH 2 —), sec-butyl ((CH 3 )(CH 3 CH 2 )CH—), t-butyl ((CH 3 ) 3 C—), n-pentyl (CH 3 CH 2 CH 2 CH 2 CH 2 —), and neopentyl ((CH 3 ) 3 CCH 2 —). 
     “Alkylene” or “alkylene” refers to divalent saturated aliphatic hydrocarbyl groups having from 1 to 10 carbon atoms and, in some embodiments, from 1 to 6 carbon atoms. “(C u -C v )alkylene” refers to alkylene groups having from u to v carbon atoms. The alkylene groups include branched and straight chain hydrocarbyl groups. For example, “(C 1 -C 6 )alkylene” is meant to include methylene, ethylene, propylene, 2-methylpropylene, dimethylethylene, pentylene, and so forth. As such, the term “propylene” could be exemplified by the following structure: 
     
       
         
         
             
             
         
       
     
     Likewise, the term “dimethylbutylene” could be exemplified by any of the following three structures or more: 
     
       
         
         
             
             
         
       
     
     Furthermore, the term “(C 1 -C 6 )alkylene” is meant to include such branched chain hydrocarbyl groups as cyclopropylmethylene, which could be exemplified by the following structure: 
     
       
         
         
             
             
         
       
     
     “Alkenyl” refers to a linear or branched hydrocarbyl group having from 2 to 10 carbon atoms and in some embodiments from 2 to 6 carbon atoms or 2 to 4 carbon atoms and having at least 1 site of vinyl unsaturation (&gt;C═C&lt;). For example, (C x -C y )alkenyl refers to alkenyl groups having from x to y carbon atoms and is meant to include for example, ethenyl, propenyl, isopropylene, 1,3-butadienyl, and the like. 
     “Alkynyl” refers to a linear monovalent hydrocarbon radical or a branched monovalent hydrocarbon radical containing at least one triple bond. The term “alkynyl” is also meant to include those hydrocarbyl groups having one triple bond and one double bond. For example, (C 2 -C 6 )alkynyl is meant to include ethynyl, propynyl, and the like. 
     “Alkoxy” refers to the group —O-alkyl wherein alkyl is defined herein. Alkoxy includes, by way of example, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, t-butoxy, sec-butoxy, and n-pentoxy. 
     “Acyl” refers to the groups H—C(O)—, alkyl-C(O)—, alkenyl-C(O)—, alkynyl-C(O)—, cycloalkyl-C(O)—, aryl-C(O)—, heteroaryl-C(O)—, and heterocyclic-C(O)—. Acyl includes the “acetyl” group CH 3 C(O)—. 
     “Acylamino” refers to the groups —NR 20 C(O)alkyl, —NR 20 C(O)cycloalkyl, —NR 20 C(O)alkenyl, —NR 20 C(O)alkynyl, —NR 20 C(O)aryl, —NR 20 C(O)heteroaryl, and —NR 20 C(O)heterocyclic, wherein R 10  is hydrogen or alkyl. 
     “Acyloxy” refers to the groups alkyl-C(O)O—, alkenyl-C(O)O—, alkynyl-C(O)O—, aryl-C(O)O—, cycloalkyl-C(O)O—, heteroaryl-C(O)O—, and heterocyclic-C(O)O—. 
     “Amino” refers to the group —NR 21 R 22  where R 21  and R 22  are independently selected from hydrogen, alkyl, alkenyl, alkynyl, aryl, cycloalkyl, heteroaryl, heterocyclic, —SO 2 -alkyl, —SO 2 -alkenyl, —SO 2 -cycloalkyl, —SO 2 -aryl, —SO 2 -heteroaryl, and —SO 2 -heterocyclic, and wherein R 21  and R 22  are optionally joined together with the nitrogen bound thereto to form a heterocyclic group. When R 21  is hydrogen and R 22  is alkyl, the amino group is sometimes referred to herein as alkylamino. When R 21  and R 22  are alkyl, the amino group is sometimes referred to herein as dialkylamino. When referring to a monosubstituted amino, it is meant that either R 21  or R 22  is hydrogen but not both. When referring to a disubstituted amino, it is meant that neither R 21  nor R 22  are hydrogen. 
     “Hydroxyamino” refers to the group —NHOH. 
     “Alkoxyamino” refers to the group —NHO-alkyl wherein alkyl is defined herein. 
     “Aminocarbonyl” refers to the group —C(O)NR 26 R 27  where R 26  and R 27  are independently selected from hydrogen, alkyl, alkenyl, alkynyl, aryl, cycloalkyl, heteroaryl, heterocyclic, hydroxy, alkoxy, amino, and acylamino, and where R 26  and R 27  are optionally joined together with the nitrogen bound thereto to form a heterocyclic group. 
     “Aryl” refers to an aromatic group of from 6 to 14 carbon atoms and no ring heteroatoms and having a single ring (e.g., phenyl) or multiple condensed (fused) rings (e.g., naphthyl or anthryl). For multiple ring systems, including fused, bridged, and spiro ring systems having aromatic and non-aromatic rings that have no ring heteroatoms, the term “Aryl” or “Ar” applies when the point of attachment is at an aromatic carbon atom (e.g., 5,6,7,8 tetrahydronaphthalene-2-yl is an aryl group as its point of attachment is at the 2-position of the aromatic phenyl ring). 
     “AUC” refers to the area under the plot of plasma concentration of drug (not logarithm of the concentration) against time after drug administration. 
     “EC 50 ” refers to the concentration of a drug that gives half-maximal response. 
     “IC 50 ” refers to the half-maximal inhibitory concentration of a drug. Sometimes, it is also converted to the pIC 50  scale (−log IC 50 ), in which higher values indicate exponentially greater potency. 
     “Clade” refers to a hypothetical construct based on experimental data. Clades are found using multiple (sometimes hundreds) of traits from a number of species (or specimens) and analyzing them statistically to find the most likely phylogenetic tree for the group. 
     “Cyano” or “nitrile” refers to the group —CN. 
     “Cycloalkyl” refers to a saturated or partially saturated cyclic group of from 3 to 14 carbon atoms and no ring heteroatoms and having a single ring or multiple rings including fused, bridged, and spiro ring systems. For multiple ring systems having aromatic and non-aromatic rings that have no ring heteroatoms, the term “cycloalkyl” applies when the point of attachment is at a non-aromatic carbon atom (e.g. 5,6,7,8,-tetrahydronaphthalene-5-yl). The term “Cycloalkyl” includes cycloalkenyl groups, such as cyclohexenyl. Examples of cycloalkyl groups include, for instance, adamantyl, cyclopropyl, cyclobutyl, cyclohexyl, cyclopentyl, cyclooctyl, cyclopentenyl, and cyclohexenyl. Examples of cycloalkyl groups that include multiple bicycloalkyl ring systems are bicyclohexyl, bicyclopentyl, bicyclooctyl, and the like. Two such bicycloalkyl multiple ring structures are exemplified and named below: 
     
       
         
         
             
             
         
       
     
     “(C u -C v )cycloalkyl” refers to cycloalkyl groups having u to v carbon atoms. 
     “Spiro cycloalkyl” refers to a 3 to 10 member cyclic substituent formed by replacement of two hydrogen atoms at a common carbon atom in a cyclic ring structure or in an alkylene group having 2 to 9 carbon atoms, as exemplified by the following structure wherein the group shown here attached to bonds marked with wavy lines is substituted with a spiro cycloalkyl group: 
     
       
         
         
             
             
         
       
     
     “Fused cycloalkyl” refers to a 3 to 10 member cyclic substituent formed by the replacement of two hydrogen atoms at different carbon atoms in a cycloalkyl ring structure, as exemplified by the following structure wherein the cycloalkyl group shown here contains bonds marked with wavy lines which are bonded to carbon atoms that are substituted with a fused cycloalkyl group: 
     
       
         
         
             
             
         
       
     
     “Carboxy” or “carboxyl” refers interchangeably to the groups 
     
       
         
         
             
             
         
       
     
     —C(O)O, or —CO 2 . 
     “Halo” or “halogen” refers to fluoro, chloro, bromo, and iodo. 
     “Haloalkyl” refers to substitution of an alkyl group with 1 to 3 halo groups (e.g., bifluoromethyl or trifluoromethyl). 
     “Haloalkoxy” refers to substitution of alkoxy groups with 1 to 5 (e.g. when the alkoxy group has at least 2 carbon atoms) or in some embodiments 1 to 3 halo groups (e.g. trifluoromethoxy). 
     “Human Serum Protein Shift Assay” refers to an HIV assay using a Luciferase Reporter to determine percent inhibition—pIC 50 . The HIV assay makes use of a two-cell co-culture system. In this assay, an infected cell line J4HxB2 and an indicator cell line HOS (delta LTR+luciferase) are co-cultured in the presence and absence of compound. The assay is designed to find inhibitors that prevent the infection of HOS cells by the J4HxB2 cell line. The assay can detect inhibitors of any stage of the HIV infection cycle. 
     “Hydroxy” or “hydroxyl” refers to the group —OH. 
     “Heteroaryl” refers to an aromatic group of from 1 to 14 carbon atoms and 1 to 6 heteroatoms selected from oxygen, nitrogen, and sulfur and includes single ring (e.g. imidazolyl) and multiple ring systems (e.g. benzimidazol-2-yl and benzimidazol-6-yl). For multiple ring systems, including fused, bridged, and spiro ring systems having aromatic and non-aromatic rings, the term “heteroaryl” applies if there is at least one ring heteroatom and the point of attachment is at an atom of an aromatic ring (e.g. 1,2,3,4-tetrahydroquinolin-6-yl and 5,6,7,8-tetrahydroquinolin-3-yl). In some embodiments, the nitrogen and/or the sulfur ring atom(s) of the heteroaryl group are optionally oxidized to provide for the N-oxide (N→O), sulfinyl, or sulfonyl moieties. More specifically the term heteroaryl includes, but is not limited to, pyridyl, furanyl, thienyl, thiazolyl, isothiazolyl, triazolyl, imidazolyl, imidazolinyl, isoxazolyl, pyrrolyl, pyrazolyl, pyridazinyl, pyrimidinyl, purinyl, phthalazyl, naphthylpryidyl, benzofuranyl, tetrahydrobenzofuranyl, isobenzofuranyl, benzothiazolyl, benzoisothiazolyl, benzotriazolyl, indolyl, isoindolyl, indolizinyl, dihydroindolyl, indazolyl, indolinyl, benzoxazolyl, quinolyl, isoquinolyl, quinolizyl, quianazolyl, quinoxalyl, tetrahydroquinolinyl, isoquinolyl, quinazolinonyl, benzimidazolyl, benzisoxazolyl, benzothienyl, benzopyridazinyl, pteridinyl, carbazolyl, carbolinyl, phenanthridinyl, acridinyl, phenanthrolinyl, phenazinyl, phenoxazinyl, phenothiazinyl, and phthalimidyl. 
     “Heterocyclic” or “heterocycle” or “heterocycloalkyl” or “heterocyclyl” refers to a saturated or partially saturated cyclic group having from 1 to 14 carbon atoms and from 1 to 6 heteroatoms selected from nitrogen, sulfur, phosphorus or oxygen and includes single ring and multiple ring systems including fused, bridged, and spiro ring systems. For multiple ring systems having aromatic and/or non-aromatic rings, the terms “heterocyclic”, “heterocycle”, “heterocycloalkyl”, or “heterocyclyl” apply when there is at least one ring heteroatom and the point of attachment is at an atom of a non-aromatic ring (e.g. 1,2,3,4-tetrahydroquinoline-3-yl, 5,6,7,8-tetrahydroquinoline-6-yl, and decahydroquinolin-6-yl). In one embodiment, the nitrogen, phosphorus and/or sulfur atom(s) of the heterocyclic group are optionally oxidized to provide for the N-oxide, phosphinane oxide, sulfinyl, sulfonyl moieties. More specifically the heterocyclyl includes, but is not limited to, tetrahydropyranyl, piperidinyl, piperazinyl, 3-pyrrolidinyl, 2-pyrrolidon-1-yl, morpholinyl, and pyrrolidinyl. A prefix indicating the number of carbon atoms (e.g., C 3 -C 10 ) refers to the total number of carbon atoms in the portion of the heterocyclyl group exclusive of the number of heteroatoms. 
     Examples of heterocycle and heteroaryl groups include, but are not limited to, azetidine, pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, pyridone, indolizine, isoindole, indole, dihydroindole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine, imidazoline, piperidine, piperazine, indoline, phthalimide, 1,2,3,4-tetrahydroisoquinoline, 4,5,6,7-tetrahydrobenzo[b]thiophene, thiazole, thiazolidine, thiophene, benzo[b]thiophene, morpholine, thiomorpholine (also referred to as thiamorpholine), piperidine, pyrrolidine, and tetrahydrofuranyl. 
     “Fused heterocyclic” or “fused heterocycle” refer to a 3 to 10 member cyclic substituent formed by the replacement of two hydrogen atoms at different carbon atoms in a cycloalkyl ring structure, as exemplified by the following structure wherein the cycloalkyl group shown here contains bonds marked with wavy lines which are bonded to carbon atoms that are substituted with a fused heterocyclic group: 
     
       
         
         
             
             
         
       
     
     “Compound”, “compounds”, “chemical entity”, and “chemical entities” as used herein refers to a compound encompassed by the generic formulae disclosed herein, any subgenus of those generic formulae, and any forms of the compounds within the generic and subgeneric formulae, including the racemates, stereoisomers, and tautomers of the compound or compounds. 
     The term “heteroatom” means nitrogen, oxygen, or sulfur and includes any oxidized form of nitrogen, such as N(O) {N + —O − } and sulfur such as S(O) and S(O) 2 , and the quaternized form of any basic nitrogen. 
     “Oxazolidinone” refers to a 5-membered heterocyclic ring containing one nitrogen and one oxygen as heteroatoms and also contains two carbons and is substituted at one of the two carbons by a carbonyl group as exemplified by any of the following structures, wherein the oxazolidinone groups shown here are bonded to a parent molecule, which is indicated by a wavy line in the bond to the parent molecule: 
     
       
         
         
             
             
         
       
     
     “Oxo” refers to a (═O) group. 
     “Polymorphism” refers to when two or more clearly different phenotypes exist in the same population of a species where the occurrence of more than one form or morph. In order to be classified as such, morphs must occupy the same habitat at the same time and belong to a panmictic population (one with random mating). 
     “Protein binding” refers to the binding of a drug to proteins in blood plasma, tissue membranes, red blood cells and other components of blood. 
     “Protein shift” refers to determining a binding shift by comparing the EC 50  values determined in the absence and presence of human serum. 
     “QVT” refers to the amino acids at positions 369, 370, and 371, respectively in the Sp1 fragment of HIV-1 Gag. 
     “Racemates” refers to a mixture of enantiomers. In an embodiment of the invention, the compounds of Formula I, or pharmaceutically acceptable salts thereof, are enantiomerically enriched with one enantiomer wherein all of the chiral carbons referred to are in one configuration. In general, reference to an enantiomerically enriched compound or salt, is meant to indicate that the specified enantiomer will comprise more than 50% by weight of the total weight of all enantiomers of the compound or salt. 
     “Solvate” or “solvates” of a compound refer to those compounds, as defined above, which are bound to a stoichiometric or non-stoichiometric amount of a solvent. Solvates of a compound includes solvates of all forms of the compound. In certain embodiments, solvents are volatile, non-toxic, and/or acceptable for administration to humans in trace amounts. Suitable solvates include water. 
     “Stereoisomer” or “stereoisomers” refer to compounds that differ in the chirality of one or more stereocenters. Stereoisomers include enantiomers and diastereomers. 
     “Tautomer” refer to alternate forms of a compound that differ in the position of a proton, such as enol-keto and imine-enamine tautomers, or the tautomeric forms of heteroaryl groups containing a ring atom attached to both a ring —NH— moiety and a ring ═N— moiety such as pyrazoles, imidazoles, benzimidazoles, triazoles, and tetrazoles. 
     The term ‘atropisomer’ refers to a stereoisomer resulting from an axis of asymmetry. This can result from restricted rotation about a single bond where the rotational barrier is high enough to allow differentiation of the isomeric species up to and including complete isolation of stable non-interconverting diastereomer or enantiomeric species. One skilled in the art will recognize that upon installing a nonsymmetrical R x  to core, the formation of atropisomers is possible. In addition, once a second chiral center is installed in a given molecule containing an atropisomer, the two chiral elements taken together can create diastereomeric and enantiomeric stereochemical species. Depending upon the substitution about the Cx axis, interconversion between the atropisomers may or may not be possible and may depend on temperature. In some instances, the atropisomers may interconvert rapidly at room temperature and not resolve under ambient conditions. Other situations may allow for resolution and isolation but interconversion can occur over a period of seconds to hours or even days or months such that optical purity is degraded measurably over time. Yet other species may be completely restricted from interconversion under ambient and/or elevated temperatures such that resolution and isolation is possible and yields stable species. When known, the resolved atropisomers were named using the helical nomenclature. For this designation, only the two ligands of highest priority in front and behind the axis are considered. When the turn priority from the front ligand 1 to the rear ligand 1 is clockwise, the configuration is P, if counterclockwise it is M. 
     “Pharmaceutically acceptable salt” refers to pharmaceutically acceptable salts derived from a variety of organic and inorganic counter ions well known in the art and include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, and tetraalkylammonium, and when the molecule contains a basic functionality, salts of organic or inorganic acids, such as hydrochloride, hydrobromide, tartrate, mesylate, acetate, maleate, and oxalate. Suitable salts include those described in P. Heinrich Stahl, Camille G. Wermuth (Eds.), Handbook of Pharmaceutical Salts Properties, Selection, and Use; 2002. 
     “Patient” or “subject” refers to mammals and includes humans and non-human mammals. 
     “Treating” or “treatment” of a disease in a patient refers to 1) preventing the disease from occurring in a patient that is predisposed or does not yet display symptoms of the disease; 2) inhibiting the disease or arresting its development; or 3) ameliorating or causing regression of the disease. 
     Wherever dashed lines occur adjacent to single bonds denoted by solid lines, then the dashed line represents an optional double bond at that position. Likewise, wherever dashed circles appear within ring structures denoted by solid lines or solid circles, then the dashed circles represent one to three optional double bonds arranged according to their proper valence taking into account whether the ring has any optional substitutions around the ring as will be known by one of skill in the art. For example, the dashed line in the structure below could either indicate a double bond at that position or a single bond at that position: 
     
       
         
         
             
             
         
       
     
     Similarly, ring A below could be a cyclohexyl ring without any double bonds or it could also be a phenyl ring having three double bonds arranged in any position that still depicts the proper valence for a phenyl ring. Likewise, in ring B below, any of X 1 -X 5  could be selected from: C, CH, or CH 2 , N, or NH, and the dashed circle means that ring B could be a cyclohexyl or phenyl ring or a N-containing heterocycle with no double bonds or a N-containing heteroaryl ring with one to three double bonds arranged in any position that still depicts the proper valence: 
     
       
         
         
             
             
         
       
     
     Where specific compounds or generic formulas are drawn that have aromatic rings, such as aryl or heteroaryl rings, then it will understood by one of still in the art that the particular aromatic location of any double bonds are a blend of equivalent positions even if they are drawn in different locations from compound to compound or from formula to formula. For example, in the two pyridine rings (A and B) below, the double bonds are drawn in different locations, however, they are known to be the same structure and compound: 
     
       
         
         
             
             
         
       
     
     The present invention includes compounds as well as their pharmaceutically acceptable salts. Accordingly, the word “or” in the context of “a compound or a pharmaceutically acceptable salt thereof” is understood to refer to either: 1) a compound alone or a compound and a pharmaceutically acceptable salt thereof (alternative), or 2) a compound and a pharmaceutically acceptable salt thereof (in combination). 
     Unless indicated otherwise, the nomenclature of substituents that are not explicitly defined herein are arrived at by naming the terminal portion of the functionality followed by the adjacent functionality toward the point of attachment. For example, the substituent “arylalkyloxycarbonyl” refers to the group (aryl)-(alkyl)-O—C(O)—. In a term such as “—C(R x ) 2 ”, it should be understood that the two R x  groups can be the same, or they can be different if R x  is defined as having more than one possible identity. In addition, certain substituents are drawn as —R x R y , where the “—” indicates a bond adjacent to the parent molecule and R y  being the terminal portion of the functionality. Similarly, it is understood that the above definitions are not intended to include impermissible substitution patterns (e.g., methyl substituted with 5 fluoro groups). Such impermissible substitution patterns are well known to the skilled artisan. 
     As recited above, Bevirimat is a yet unapproved anti-HIV drug derived from a betulinic acid-like compound, first isolated from  Syzygium claviflorum,  a Chinese herb. It is believed to inhibit HIV by a novel mechanism, so-called maturation inhibition. Like protease inhibitors, Bevirimat and other maturation inhibitors interfere with protease processing of newly translated HIV polyprotein precursor, called gag. Gag is an essential structural protein of the HIV virus. Gag undergoes a chain of interactions both with itself and with other cellular and viral factors to accomplish the assembly of infectious virus particles. 
     However, naturally occurring polymorphisms in HIV are present in some infected individuals, thus lowering the anti-HIV efficacy of some currently considered therapies. Indeed, studies have shown that presence of a number of single nucleotide polymorphisms in the Capsid/SP1 spacer protein (CA/SP1) cleavage site has resulted in clinical resistance in HIV patients to Bevirimat. Likewise, mutations in the glutamine-valine-threonine (QVT) motif of the SP1 peptide are also known to cause Bevirimat resistance in HIV infected patients. Mutations in the QVT motif of the SP1 peptide are the primary predictors of failure to respond to Bevirimat and the effect of these mutations has been repeatedly demonstrated. These problems eventually led to the cessation of clinical development of Bevirimat. See Knapp, D., et al.,  J. Clin. Microbiol.  49(1): 201-208 (2011). 
     Bevirimat: 
     
       
         
         
             
             
         
       
     
     Bevirimat&#39;s Clinical Problems: 
     
         
         
           
             Polymorphism issues &amp; weak potency. 
             MT4 antiviral assay NL4-3 strain EC 50 =223 nM. 
             MT4 antiviral assay NL4-3 strain with V370A site directed mutant polymorphism EC 50 =6062 nM. 
             In assay fold shift with human serum 157 fold. See Table 6. 
             C min  target&gt;20 μg/mL*. 
             &gt;40% of Glade B patients have QVT polymorphisms*.
 
*See McCallister, et al., XVII International Drug Resistance Workshop, Jun. 10-14, 2008, Sitges, Spain. Conference poster “ HIV -1  Gag Polymorphisms Determine Treatment Response to Bevirimat  (PA-457)”.
 
           
         
       
    
     After the above HIV clinical problems with Bevirimat were reported, several new HIV active maturation inhibitor compounds were discovered. For example, certain maturation inhibitor compounds (hereinafter, compounds “A”, “B” and “C”, as shown below) have been described in PCT Published Application No. WO2011/100308 and PCT Application Serial No. PCT/CN2011/001302. In addition, the present application also describes compounds 51 and 56, among others, as detailed throughout. The present application describes compounds that are novel over the compounds described in PCT Published Application No. WO2011/100308 and PCT Application Serial No. PCT/CN2011/001302. In addition, certain compounds described herein show unexpectedly superior properties over the compounds (“A”, “B”, and “C”) described in PCT Published Application No. WO2011/100308 and PCT Application Serial No. PCT/CN2011/001302. 
     One difference between the compounds described in those two references and the compounds of the present application is that both of those references have compounds requiring a carbonyl group at a position where, instead, the present application application describes compounds that cannot have a carbonyl at the same position. By way of example only, this carbonyl versus non-carbonyl difference is highlighted by the arrows indicated directly below. The generic structures of Formulas (I) and (II) bear this out within the present application because when W is oxygen, there can only be a single bond between the adjacent carbon and the W. In sum, there can be no double bond between W and its adjacent carbon so as to form a carbonyl in the Formulas of the present application. 
     
       
         
         
             
             
         
       
     
     Indeed, this structural difference has now been discovered to unexpectedly improve many of the properties that are involved with creating an efficacious drug for the prevention and/or treatment of viral diseases, such as HIV. One or more of such properties of certain compounds described within the present application, include, but are not limited to, improving the HIV virus polymorphism coverage, improving the in vitro potency (EC 50 ), reducing the projected clinical human AUC target, potentially reducing any toxicity window by lowering the required dosage to be efficacious, and reducing the impact of protein binding and/or serum shift upon the projected clinical AUC target. 
     Such improvements to the pharmacokinetics and projected clinical use of certain compounds described herein are described in more detail within Examples 84-89 below. 
     
       
         
         
             
             
         
       
     
     In accordance with one embodiment of the present invention, there is provided a compound having the structure of Formula I: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are independently selected from a bond or [C(R 6 R 6′ )] q ; 
     each instance of Q is independently selected from —CH 2 — or —C(═O)—; 
     W is selected from a bond or O; 
     R 1  is selected from the group consisting of —H, —C(O)R 5 , —CH 2 —O—(C 1 -C 6 )alkyl, 2-tetrahydro-2H-pyran, and 
     
       
         
         
             
             
         
       
     
     R 2  is selected from the group consisting of —H, —(C 1 -C 6 )alkyl-OR 4 , —(C 1 -C 6 )alkyl-O—(C 1 -C 6 )alkyl, —C(O)R 5 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r N + (R 4 ) 3 , wherein when W is O, R 1  and R 2  can optionally be taken together with the O and N to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring, wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 3  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 2  and R 3  can optionally be taken together with the nitrogen and L 2  to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring, wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —R 3 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 . 
     R 6  and R 6′  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —Y, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 , wherein the R 6  and R 6′  groups can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring, and wherein the cycloalkyl ring may be optionally substituted by one to three R 11  groups; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, -Q-aryl-(R 4 ) n , —NR 14 R 15 , —C(O)CH 3 , wherein R 7  and R 8  can optionally be taken together with the nitrogen to which they are joined to form a 4 to 8 membered heterocyclyl or heteroaryl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 11  groups; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , —CO(O)R 4 , and —CO(O)R 6 , wherein any two R 11 , R 12  or R 13  groups can optionally join to form a 3 to 8 membered cycloalkyl, aryl, heterocyclyl or heteroaryl ring, wherein the heterocyclyl or heteroaryl ring may contain one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, and wherein the cycloalkyl, aryl, heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 16  groups; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 , wherein R 14  and R 15  can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring or 4 to 8 membered heterocyclyl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the cycloalkyl ring or heterocyclyl ring may be optionally substituted by one to three R 16  groups; 
     R 16  is independently selected from the group consisting of —H, halo, oxo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocycle, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, 2, 3, or 4; 
     p is independently 0, 1, 2, 3, or 4; and 
     r and q in each instance are independently 0, 1, 2, 3, or 4. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are [C(R 6 R 6′ )] q ; 
     each Q is independently selected from —CH 2 — or —C(═O)—; 
     W is selected from a bond or O; 
     R 1  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —C(O)R 4 , and 
     
       
         
         
             
             
         
       
     
     R 2  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —(C 1 -C 6 )alkyl-OR 4 , —(C 1 -C 6 )alkyl-O—(C 1 -C 6 )alkyl, —C(O)R 5 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r N + (R 4 ) 3 ; 
     R 3  is selected from the group consisting of hydrogen, (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of (C 1 -C 6 )alkyl, —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 ; 
     R 6  and R 6′  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 , wherein the R 6  and R 6′  groups can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring, and wherein the cycloalkyl ring may be optionally substituted by one to three R 11  groups; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, —NR 14 R 15 , and —C(O)CH 3 ; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , —CO(O)R 4 , and —CO(O)R 5 ; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 ; 
     R 16  is independently selected from the group consisting of —H, oxo, halo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocycle, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, 2, 3, or 4; 
     p is independently 0, 1, 2, 3, or 4; and 
     r and q in each instance are independently 0, 1, 2, 3, or 4. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are both (—CH 2 —); 
     Q is —C(═O)—; 
     W is O; 
     R 1  is —H; 
     R 2  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —(C 1 -C 6 )alkyl-OR 4 , —(C 1 -C 6 )alkyl-O—(C 1 -C 6 )alkyl, —C(O)R 5 , and —(CH 2 ) r NR 7 R 8 ; 
     R 3  is selected from the group consisting of (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of (C 1 -C 6 )alkyl, —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 ; 
     R 6  and R 6′  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 ; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, —NR 14 R 15 , and —C(O)CH 3 ; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , —CO(O)R 4 , and —CO(O)R 5 ; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 ; 
     R 16  is independently selected from the group consisting of —H, oxo, halo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocyclyl, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, or 2; 
     p is independently 0, 1, or 2; and 
     r and q in each instance are independently 0, 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are both (—CH 2 —); 
     Q is —C(═O)—; 
     W is O; 
     R 1  is —H; 
     R 2  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —C(O)R 5 , and —(CH 2 ) r NR 7 R 8 ; 
     R 3  is 
     
       
         
         
             
             
         
       
     
     wherein X is a monocyclic or bicyclic (C 5 -C 14 )aryl; 
     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of (C 1 -C 6 )alkyl, —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 ; 
     R 6  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 ; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, —NR 14 R 15 , and —C(O)CH 3 ; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —CO(O)R 4 , and —CO(O)R 5 ; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 ; 
     R 16  is independently selected from the group consisting of —H, oxo, halo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocyclyl, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, or 2; 
     p is independently 0, 1, or 2; and 
     r and q in each instance are independently 0, 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I: 
     (I) 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are both (—CH 2 —); 
     Q is —C(═O)—; 
     W is O; 
     R 1  is —H; 
     R 2  is —(CH 2 ) r NR 7 R 8 ; 
     R 3  is 
     
       
         
         
             
             
         
       
     
     wherein X is phenyl; 
     R 7  and R 8  are independently selected from the group consisting of —H and methyl; 
     R 9  is selected from the group consisting of chloro, bromo, and fluoro; 
     R 11  is selected from the group consisting of chloro, bromo, and fluoro; 
     m is 0, 1, or 2; and 
     r is 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula II: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are independently selected from a bond or [C(R 6 R 6′ )] q ; 
     each instance of Q is independently selected from —CH 2 — or —C(═O)—; 
     W is selected from a bond or O; 
     R 1  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —C(O)R 5 , —CH 2 —O—(C 1 -C 6 )alkyl, 2-tetrahydro-2H-pyran, and 
     
       
         
         
             
             
         
       
     
     R 2  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —(C 1 -C 6 )alkyl-OR 4 , —(C 1 -C 6 )alkyl-O—(C 1 -C 6 )alkyl, —C(O)R 5 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r N + (R 4 ) 3 , wherein when W is O, R 1  and R 2  can optionally be taken together with the O and N to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring, wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 3  is selected from the group consisting of hydrogen, (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 2  and R 3  can optionally be taken together with the nitrogen and L 2  to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —R 3 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 . 
     R 6  and R 6′  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —Y, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 , wherein the R 6  and R 6′  groups can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring, and wherein the cycloalkyl ring may be optionally substituted by one to three R 11  groups; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, -Q-aryl-(R 4 ) n , —NR 14 R 15 , —C(O)CH 3 , wherein R 7  and R 8  can optionally be taken together with the nitrogen to which they are joined to form a 4 to 8 membered heterocyclyl or heteroaryl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 11  groups; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , —CO(O)R 4 , and —CO(O)R 5 , wherein any two R 11 , R 12  or R 13  groups can optionally join to form a 3 to 8 membered cycloalkyl, aryl, heterocyclyl or heteroaryl ring, wherein the heterocyclyl or heteroaryl ring may contain one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, and wherein the cycloalkyl, aryl, heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 16  groups; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 , wherein R 14  and R 15  can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring or 4 to 8 membered heterocyclyl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the cycloalkyl ring or heterocyclyl ring may be optionally substituted by one to three R 16  groups; 
     R 16  is independently selected from the group consisting of —H, halo, oxo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocycle, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, 2, 3, or 4; 
     p is independently 0, 1, 2, 3, or 4; and 
     r and q in each instance are independently 0, 1, 2, 3, or 4. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula II: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are [C(R 6 R 6′ )] q ; 
     each Q is independently selected from—CH 2 — or —C(═O)—; 
     W is selected from a bond or O; 
     R 1  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —C(O)R 4 , and 
     
       
         
         
             
             
         
       
     
     R 2  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —(C 1 -C 6 )alkyl-OR 4 , —(C 1 -C 6 )alkyl-O—(C 1 -C 6 )alkyl, —C(O)R 5 , —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r N + (R 4 ) 3 ; 
     R 3  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of (C 1 -C 6 )alkyl, —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 ; 
     R 6  and R 6′  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 , wherein the R 6  and R 6′  groups can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring, and wherein the cycloalkyl ring may be optionally substituted by one to three R 11  groups; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, —NR 14 R 15 , and —C(O)CH 3 ; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , —CO(O)R 4 , and —CO(O)R 5 ; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 ; 
     R 16  is independently selected from the group consisting of —H, oxo, halo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocycle, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, 2, 3, or 4; 
     p is independently 0, 1, 2, 3, or 4; and 
     r and q in each instance are independently 0, 1, 2, 3, or 4. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula II: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are both (—CH 2 —); 
     Q is —C(═O)—; 
     W is O; 
     R 1  is —H; 
     R 2  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, —C(O)R 5 , and —(CH 2 ) r NR 7 R 8 ; 
     R 3  is 
     
       
         
         
             
             
         
       
     
     wherein X is a monocyclic or bicyclic (C 5 -C 14 )aryl; 
     R 4  is selected from the group consisting of —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from the group consisting of (C 1 -C 6 )alkyl, —(CH 2 ) r NR 7 R 8 , and —(CH 2 ) r OR 7 ; 
     R 6  is selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —(CH 2 ) r NR 7 R 8 , —C(O)OH, and —C(O)NH 2 ; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, —NR 14 R 15 , and —C(O)CH 3 ; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —CO(O)R 4 , and —CO(O)R 5 ; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 ; 
     R 16  is independently selected from the group consisting of —H, oxo, halo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocycle, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 8 (R 6 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, or 2; 
     p is independently 0, 1, or 2; and 
     r and q in each instance are independently 0, 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula II: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are both (—CH 2 —); 
     Q is —C(═O)—; 
     W is O; 
     R 1  is —H; 
     R 2  is —(CH 2 ) r NR 7 R 8 ; 
     R 3  is 
     
       
         
         
             
             
         
       
     
     wherein X is phenyl; 
     R 7  and R 8  are independently selected from the group consisting of —H and methyl; 
     R 9  is selected from the group consisting of chloro, bromo, and fluoro; 
     R 11  is selected from the group consisting of chloro, bromo, and fluoro; 
     m is 0, 1, or 2; and 
     r is 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound of Formula II: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein: 
     A is 
     
       
         
         
             
             
         
       
     
     L 1  and L 2  are independently selected from a bond or [C(R 6 R 6 )] q ; 
     Q is selected from —CH 2 — or —C(═O)—; 
     W is selected from a bond or oxygen; 
     R 1  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —C(O)R 5 , —CH 2 —O—(C 1 -C 6 )alkyl, 2-tetrahydro-2H-pyran and 
     
       
         
         
             
             
         
       
     
     R 2  is selected from the group consisting of —H, (C 1 -C 12 )alkyl, —C 1-6  alkyl-OH, —C 1-6  alkyl-O—C 1-6  alkyl, —C(O)R 5 , and —(CH 2 ) r NR 7 R 8 , —C(O)R 5 , wherein when W is oxygen, R 1  and R 2  can optionally be taken together with the oxygen and nitrogen to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 3  is selected from the group consisting of hydrogen, (C 1 -C 12 )alkyl, —NR 1 R 2 , —OR 5 , 
     
       
         
         
             
             
         
       
     
     wherein:
         X is a monocyclic or bicyclic (C 5 -C 14 )aryl,   Y is selected from a monocyclic or bicyclic (C 2 -C 9 )heterocyclyl or monocyclic or bicyclic (C 2 -C 9 )heteroaryl, each having one to three heteroatoms selected from S, N or O, and   Z is a monocyclic or bicyclic (C 3 -C 8 )cycloalkyl;       

     R 2  and R 3  can optionally be taken together with the nitrogen and L 2  to which they are respectively joined to form a 4 to 8 membered heterocyclyl ring, wherein the heterocyclyl ring may be optionally substituted by one to two R 11  groups; 
     R 4  is selected from —H and (C 1 -C 6 )alkyl; 
     R 5  is selected from (C 1 -C 6 )alkyl, —R 3 , —(CH 2 ) r NR 7 R 8 , or —(CH 2 ) r OR 7 ; 
     R 6  and R 6′  are independently —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, haloalkyl, —Y, —(CH 2 ) r NR 7 R 8 , —C(O)OH, —C(O)NH 2 , wherein the R 6  and R 6′  groups can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring, wherein the cycloalkyl ring may be optionally substituted by one to three R 11  groups; 
     R 7  and R 8  are independently selected from the group consisting of —H, (C 1 —C— 6 )alkyl, (C 3 -C 8 )cycloalkyl, -Q-aryl-(R 4 ) n , —NR 14 R 15 , —C(O)CH 3 , wherein R 7  and R 8  can optionally be taken together with the nitrogen to which they are joined to form a 4 to 8 membered heterocyclyl or heteroaryl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 11  groups; 
     R 9  is halo; 
     R 10  is —N(R 16 ) 2 ; 
     R 11 , R 12 , and R 13  are independently selected from the group consisting of oxo, hydroxyl, halo, (C 1 -C 6 )alkoxy, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , nitro, —SO 2 R 6 , (C 1 -C 6 )alkyl, —C(O)R 10 , —R 4 YR 6 , and —CO(O)R 5 , wherein any two R 9 , R 10  or R 11  groups can optionally join to form a 3 to 8 membered cycloalkyl, aryl, heterocyclyl or heteroaryl ring, wherein the heterocyclyl or heteroaryl ring may contain one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, and wherein the cycloalkyl, aryl, heterocyclyl or heteroaryl ring may be optionally substituted by one to three R 16  groups; 
     R 14  and R 15  are independently selected from the group consisting of —H, (C 1 -C 6 )alkyl, (C 3 -C 8 )cycloalkyl, (C 1 -C 6 )alkoxy, —[C(R 6 ) 2 ] r —, —O[C(R 6 ) 2 ] r —, oxo, hydroxyl, halo, —C(O)R 7 , —R 10 , and —CO(O)R 2 , wherein R 14  and R 15  can optionally be taken together with the carbon to which they are joined to form a 3 to 8 membered cycloalkyl ring or 4 to 8 membered heterocyclyl ring containing one to three heteroatoms selected from —NR 5 —, —O—, —S—, —S(O)—, or —SO 2 —, wherein the cycloalkyl ring or heterocyclyl ring may be optionally substituted by one to three R 16  groups; 
     R 16  is independently selected from the group consisting of halo, oxo, hydroxyl, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, (C 3 -C 8 )cycloalkyl, —R 6 (R 9 ) q , —OR 6 (R 9 ) q , —N(R 4 ) 2 , —(CH 2 ) r -heterocyclyl, —C(O)OH, —C(O)NH 2 , —R 5 (R 9 ) q , —OR 5 (R 9 ) q , nitro, —SO 2 R 6 , —C(O)R 10 , and —CO(O)R 4 ; 
     m and n in each instance are independently 0, 1, 2, 3, or 4; 
     p is independently 0, 1, 2, 3, or 4; and 
     r and q in each instance are independently 0, 1, 2, 3, or 4. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein L 1  and L 2  are both [C(R 6 R 6′ )] q . 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein L 1  and L 2  are both —CH 2 —. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein q is independently 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein q is 1. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein W is O. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein W is a bond. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein when W is a bond, then R 1  is —H. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein when W is O, then R 1  is —H. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein Q m  in the A group is absent and Q, in the A group is —CH 2 — and the Q in the Formula I structure is —C(═O)—. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 1  is —H. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 2  is —(CH 2 ) r NR 7 R 8 . 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 2  is (dimethylamino)ethyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein r is independently 0, 1, 2, or 3. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein r is 2. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 3  is 
     
       
         
         
             
             
         
       
     
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein X is a monocyclic (C 5 -C 14 )aryl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein X is phenyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 4  is —H. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein m is 0 or 1. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein m is 0. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein m is 1. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein n is 1. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 6  and R 6′  are both —H. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 7  and R 8  are both (C 1 -C 6 )alkyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 7  is methyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 8  is methyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 7  and R 8  are both methyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 11  is halo. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 11  is selected from chloro, bromo, or fluoro. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 11  is chloro. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 11  is absent. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 14  and R 15  are both (C 1 -C 6 )alkyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein R 14  and R 15  are both methyl. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein A is 
     
       
         
         
             
             
         
       
     
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein A is 
     
       
         
         
             
             
         
       
     
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein A is 
     
       
         
         
             
             
         
       
     
     In accordance with another embodiment of the present invention, there is provided a compound having the structure of Formula I or Formula II above, wherein A is 
     
       
         
         
             
             
         
       
     
     In accordance with another embodiment of the present invention, there is provided a compound having the structure: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In accordance with another embodiment of the present invention, there is provided a compound having the structure: 
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof. 
     In a further embodiment of the present invention, there is provided a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable excipient. 
     In a further embodiment of the present invention, there is provided a method of treating HIV comprising administering to a patient suffering therefrom an effective amount of a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof. 
     In a further embodiment of the present invention, there is provided a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient. 
     In a further embodiment of the present invention, there is provided a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, wherein the compound is present in an amorphous form. 
     In a further embodiment of the present invention, there is provided a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, wherein the composition is in a tablet form. 
     In a further embodiment of the present invention, there is provided a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, wherein the compound is present as a spray dried dispersion. 
     In a further embodiment of the present invention, there is provided a method of treating an HIV infection in a subject comprising administering to the subject a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof. In certain embodiments, the subject is a mammal, and in other embodiments, the subject is a human. 
     In a further embodiment of the present invention, there is provided a method of treating an HIV infection in a subject comprising administering to the subject a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient. 
     In a further embodiment of the present invention, there is provided a method of preventing an HIV infection in a subject at risk for developing an HIV infection, comprising administering to the subject a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof. 
     In a further embodiment of the present invention, there is provided a method of preventing an HIV infection in a subject at risk for developing an HIV infection, comprising administering to the subject a pharmaceutical composition comprising a compound of Formula I or Formula II, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient. 
     In still other embodiments, the present invention also provides the use of a compound or salt as defined in any of Formula I or Formula II in the manufacture of a medicament for use in the treatment of an HIV infection in a human. 
     Furthermore, the compounds of the invention can exist in particular geometric or stereoisomeric forms. The invention contemplates all such compounds, including cis- and trans-isomers, (−)- and (+)-enantiomers, (R)- and (S)-enantiomers, diastereomers, (D)-isomers, (L)-isomers, the racemic mixtures thereof, and other mixtures thereof, such as enantiomerically or diastereomerically enriched mixtures, as falling within the scope of the invention. Additional asymmetric carbon atoms can be present in a substituent such as an alkyl group. All such isomers, as well as mixtures thereof, are intended to be included in this invention. 
     Optically active (R)- and (S)-isomers and d and I isomers can be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques. If, for instance, a particular enantiomer of a compound of the present invention is desired, it can be prepared by asymmetric synthesis, or by derivatization with a chiral auxiliary, where the resulting diastereomeric mixture is separated and the auxiliary group cleaved to provide the pure desired enantiomers. Alternatively, where the molecule contains a basic functional group, such as an amino group, or an acidic functional group, such as a carboxyl group, diastereomeric salts can be formed with an appropriate optically active acid or base, followed by resolution of the diastereomers thus formed by fractional crystallization or chromatographic means known in the art, and subsequent recovery of the pure enantiomers. In addition, separation of enantiomers and diastereomers is frequently accomplished using chromatography employing chiral, stationary phases, optionally in combination with chemical derivatization (e.g., formation of carbamates from amines). 
     In another embodiment of the invention, there is provided a compound of Formula I or Formula II, wherein the compound or salt of the compound is used in the manufacture of a medicament for use in the treatment of a viral infection in a human. 
     In another embodiment of the invention, there is provided a pharmaceutical composition comprising a pharmaceutically acceptable diluent and a therapeutically effective amount of a compound as defined in Formula I. 
     In one embodiment, the pharmaceutical formulation containing a compound of Formula I or Formula II or a salt thereof is a formulation adapted for parenteral administration. In another embodiment, the formulation is a long-acting parenteral formulation. In a further embodiment, the formulation is a nano-particle formulation. 
     The compounds of the present invention and their salts, solvates, or other pharmaceutically acceptable derivatives thereof, may be employed alone or in combination with other therapeutic agents. Therefore, in other embodiments, the methods of treating and/or preventing an HIV infection in a subject may in addition to administration of a compound of Formula I or Formula II further comprise administration of one or more additional pharmaceutical agents active against HIV. 
     In such embodiments, the one or more additional agents active against HIV is selected from the group consisting of zidovudine, didanosine, lamivudine, zalcitabine, abacavir, stavudine, adefovir, adefovir dipivoxil, fozivudine, todoxil, emtricitabine, alovudine, amdoxovir, elvucitabine, nevirapine, delavirdine, efavirenz, loviride, immunocal, oltipraz, capravirine, lersivirine, GSK2248761, TMC-278, TMC-125, etravirine, saquinavir, ritonavir, indinavir, nelfinavir, amprenavir, fosamprenavir, brecanavir, darunavir, atazanavir, tipranavir, palinavir, lasinavir, enfuvirtide, T-20, T-1249, PRO-542, PRO-140, TNX-355, BMS-806, BMS-663068 and BMS-626529, 5-Helix, raltegravir, elvitegravir, GSK1349572, GSK1265744, vicriviroc (Sch-C), Sch-D, TAK779, maraviroc, TAK449, didanosine, tenofovir, lopinavir, and darunavir. 
     As such, the compounds of the present invention and any other pharmaceutically active agent(s) may be administered together or separately and, when administered separately, administration may occur simultaneously or sequentially, in any order. The amounts of the compounds of the present invention and the other pharmaceutically active agent(s) and the relative timings of administration will be selected in order to achieve the desired combined therapeutic effect. The administration in combination of a compound of the present invention and salts, solvates, or other pharmaceutically acceptable derivatives thereof with other treatment agents may be in combination by administration concomitantly in: (1) a unitary pharmaceutical composition including both compounds; or (2) separate pharmaceutical compositions each including one of the compounds. Alternatively, the combination may be administered separately in a sequential manner wherein one treatment agent is administered first and the other second or vice versa. Such sequential administration may be close in time or remote in time. The amounts of the compound(s) of Formula I or Formula II or salts thereof and the other pharmaceutically active agent(s) and the relative timings of administration will be selected in order to achieve the desired combined therapeutic effect. 
     In addition, the compounds of the present invention may be used in combination with one or more other agents useful in the prevention or treatment of HIV. 
     Examples of such agents include: 
     Nucleotide reverse transcriptase inhibitors such as zidovudine, didanosine, lamivudine, zalcitabine, abacavir, stavudine, adefovir, adefovir dipivoxil, fozivudine, todoxil, emtricitabine, alovudine, amdoxovir, elvucitabine, and similar agents; 
     Non-nucleotide reverse transcriptase inhibitors (including an agent having anti-oxidation activity such as immunocal, oltipraz, etc.) such as nevirapine, delavirdine, efavirenz, loviride, immunocal, oltipraz, capravirine, lersivirine, GSK2248761, TMC-278, TMC-125, etravirine, and similar agents; 
     Protease inhibitors such as saquinavir, ritonavir, indinavir, nelfinavir, amprenavir, fosamprenavir, brecanavir, darunavir, atazanavir, tipranavir, palinavir, lasinavir, and similar agents; 
     Entry, attachment and fusion inhibitors such as enfuvirtide (T-20), T-1249, PRO-542, PRO-140, TNX-355, BMS-806, BMS-663068 and BMS-626529, 5-Helix and similar agents; 
     Integrase inhibitors such as raltegravir, elvitegravir, GSK1349572, GSK1265744 and similar agents; 
     Maturation inhibitors such as PA-344 and PA-457, and similar agents; and 
     CXCR4 and/or CCR5 inhibitors such as vicriviroc (Sch-C), Sch-D, TAK779, maraviroc (UK 427,857), TAK449, as well as those disclosed in WO 02/74769, PCT/US03/39644, PCT/US03/39975, PCT/US03/39619, PCT/US03/39618, PCT/US03/39740, and PCT/US03/39732, and similar agents. 
     Further examples where the compounds of the present invention may be used in combination with one or more agents useful in the prevention or treatment of HIV are found in Table 1. 
     
       
         
           
               
               
               
               
             
               
                 TABLE 1 
               
               
                   
               
               
                   
                 Brand 
                   
                   
               
               
                 FDA Approval 
                 Name 
                 Generic Name 
                 Manufacturer 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
            
               
                 Nucleoside Reverse Transcriptase 
               
               
                 Inhibitors (NRTIs) 
               
            
           
           
               
               
               
               
            
               
                 1987 
                 Retrovir 
                 zidovudine, 
                 GlaxoSmithKline 
               
               
                   
                   
                 azidothymidine, 
                   
               
               
                   
                   
                 AZT, ZDV 
                   
               
               
                 1991 
                 Videx 
                 didanosine, 
                 Bristol-Myers 
               
               
                   
                   
                 dideoxyinosine, 
                 Squibb 
               
               
                   
                   
                 ddl 
                   
               
               
                 1992 
                 Hivid 
                 zalcitabine, 
                 Roche 
               
               
                   
                   
                 dideoxycytidine, 
                 Pharmaceuticals 
               
               
                   
                   
                 ddC 
                   
               
               
                 1994 
                 Zerit 
                 stavudine, d4T 
                 Bristol-Myers 
               
               
                   
                   
                   
                 Squibb 
               
               
                 1995 
                 Epivir 
                 lamivudine, 3TC 
                 GlaxoSmithKline 
               
               
                 1997 
                 Combivir 
                 lamivudine + 
                 GlaxoSmithKline 
               
               
                   
                   
                 zidovudine 
                   
               
               
                 1998 
                 Ziagen 
                 abacavir sulfate, 
                 GlaxoSmithKline 
               
               
                   
                   
                 ABC 
                   
               
               
                 2000 
                 Trizivir 
                 abacavir + 
                 GlaxoSmithKline 
               
               
                   
                   
                 lamivudine + 
                   
               
               
                   
                   
                 zidovudine 
                   
               
               
                 2000 
                 Videx EC 
                 enteric coated 
                 Bristol-Myers 
               
               
                   
                   
                 didanosine, ddl 
                 Squibb 
               
               
                   
                   
                 EC 
                   
               
               
                 2001 
                 Viread 
                 tenofovir 
                 Gilead Sciences 
               
               
                   
                   
                 disoproxil 
                   
               
               
                   
                   
                 fumarate, TDF 
                   
               
               
                 2003 
                 Emtriva 
                 emtricitabine, FTC 
                 Gilead Sciences 
               
               
                 2004 
                 Epzicom 
                 abacavir + 
                 GlaxoSmithKline 
               
               
                   
                   
                 lamivudine 
                   
               
               
                 2004 
                 Truvada 
                 emtricitabine + 
                 Gilead Sciences 
               
               
                   
                   
                 tenofovir 
                   
               
               
                   
                   
                 disoproxil 
                   
               
               
                   
                   
                 fumarate 
                   
               
            
           
           
               
            
               
                 Non-Nucleosides Reverse 
               
               
                 Transcriptase Inhibitors (NNRTIs) 
               
            
           
           
               
               
               
               
            
               
                 1996 
                 Viramune 
                 nevirapine, NVP 
                 Boehringer 
               
               
                   
                   
                   
                 Ingelhelm 
               
               
                 1997 
                 Rescriptor 
                 delavirdine, DLV 
                 Pfizer 
               
               
                 1998 
                 Sustiva 
                 efavirenz, EFV 
                 Bristol-Myers 
               
               
                   
                   
                   
                 Squibb 
               
               
                 2008 
                 Intelence 
                 etravirine 
                 Tibotec 
               
               
                   
                   
                   
                 Therapeutics 
               
            
           
           
               
            
               
                 Protease Inhibitors (PIs) 
               
            
           
           
               
               
               
               
            
               
                 1995 
                 Invirase 
                 saquinavir 
                 Roche 
               
               
                   
                   
                 mesylate, SQV 
                 Pharmaceuticals 
               
               
                 1996 
                 Norvir 
                 ritonavir, RTV 
                 Abbott 
               
               
                   
                   
                   
                 Laboratories 
               
               
                 1996 
                 Crixivan 
                 indinavir, IDV 
                 Merck 
               
               
                 1997 
                 Viracept 
                 nelfinavir 
                 Pfizer 
               
               
                   
                   
                 mesylate, NFV 
                   
               
               
                 1997 
                 Fortovase 
                 saquinavir (no 
                 Roche 
               
               
                   
                   
                 longer marketed) 
                 Pharmaceuticals 
               
               
                 1999 
                 Agenerase 
                 amprenavir, APV 
                 GlaxoSmithKline 
               
               
                 2000 
                 Kaletra 
                 lopinavir + 
                 Abbott 
               
               
                   
                   
                 ritonavir, 
                 Laboratories 
               
               
                   
                   
                 LPV/RTV 
                   
               
               
                 2003 
                 Reyataz 
                 atazanavir sulfate, 
                 Bristol-Myers 
               
               
                   
                   
                 ATV 
                 Squibb 
               
               
                 2003 
                 Lexiva 
                 fosamprenavir 
                 GlaxoSmithKline 
               
               
                   
                   
                 calcium, FOS- 
                   
               
               
                   
                   
                 APV 
                   
               
               
                 2005 
                 Aptivus 
                 tripranavir, TPV 
                 Boehringer 
               
               
                   
                   
                   
                 Ingelhelm 
               
               
                 2006 
                 Prezista 
                 darunavir 
                 Tibotec 
               
               
                   
                   
                   
                 Therapeutics 
               
            
           
           
               
            
               
                 Fusion Inhibitors 
               
            
           
           
               
               
               
               
            
               
                 2003 
                 Fuzeon 
                 Enfuvirtide, T-20 
                 Roche 
               
               
                   
                   
                   
                 Pharmaceuticals 
               
               
                   
                   
                   
                 &amp; Trimeris 
               
            
           
           
               
            
               
                 Entry Inhibitors 
               
            
           
           
               
               
               
               
            
               
                 2007 
                 Selzentry 
                 maraviroc 
                 Pfizer 
               
            
           
           
               
            
               
                 Integrase Inhibitors 
               
            
           
           
               
               
               
               
            
               
                 2007 
                 Isentress 
                 raltegravir 
                 Merck 
               
               
                   
               
            
           
         
       
     
     The scope of combinations of compounds of this invention with HIV agents is not limited to those mentioned above, but includes in principle any combination with any pharmaceutical composition useful for the treatment of HIV. As noted, in such combinations the compounds of the present invention and other HIV agents may be administered separately or in conjunction. In addition, one agent may be prior to, concurrent to, or subsequent to the administration of other agent(s). 
     The present invention may be used in combination with one or more agents useful as pharmacological enhancers as well as with or without additional compounds for the prevention or treatment of HIV. Examples of such pharmacological enhancers (or pharmakinetic boosters) include, but are not limited to, ritonavir, GS-9350, and SPI-452. 
     Ritonavir is 10-hydroxy-2-methyl-5-(1-methylethyl)-1-1[2-(1-methylethyl)-4-thiazolyl]-3,6-dioxo-8,11-bis(phenylmethyl)-2,4,7,12-tetraazatridecan-13-oic acid, 5-thiazolylmethyl ester, [5S-(5S*,8R*,10R*,11R*)] and is available from Abbott Laboratories of Abbott park, Ill., as Norvir. Ritonavir is an HIV protease inhibitor indicated with other antiretroviral agents for the treatment of HIV infection. Ritonavir also inhibits P450 mediated drug metabolism as well as the P-glycoprotein (Pgp) cell transport system, thereby resulting in increased concentrations of active compound within the organism. 
     GS-9350 is a compound being developed by Gilead Sciences of Foster City, Ca. as a pharmacological enhancer. 
     SPI-452 is a compound being developed by Sequoia Pharmaceuticals of Gaithersburg, Md., as a pharmacological enhancer. 
     In one embodiment of the present invention, a compound of Formula I or Formula II is used in combination with ritonavir. In one embodiment, the combination is an oral fixed dose combination. In another embodiment, the compound of Formula I or Formula II is formulated as a long acting parenteral injection and ritonavir is formulated as an oral composition. In one embodiment, is a kit containing the compound of Formula I or Formula II formulated as a long acting parenteral injection and ritonavir formulated as an oral composition. In another embodiment, the compound of Formula I or Formula II is formulated as a long acting parenteral injection and ritonavir is formulated as an injectable composition. In one embodiment, is a kit containing the compound of Formula I or Formula II formulated as a long acting parenteral injection and ritonavir formulated as an injectable composition. 
     In another embodiment of the present invention, a compound of Formula I or Formula II is used in combination with GS-9350. In one embodiment, the combination is an oral fixed dose combination. In another embodiment, the compound of Formula I or Formula II is formulated as a long acting parenteral injection and GS-9350 is formulated as an oral composition. In one embodiment, there is provided a kit containing the compound of Formula I or Formula II formulated as a long acting parenteral injection and GS-9350 formulated as an oral composition. In another embodiment, the compound of Formula I or Formula II is formulated as a long acting parenteral injection and GS-9350 is formulated as an injectable composition. In one embodiment, is a kit containing the compound of Formula I or Formula II is formulated as a long acting parenteral injection and GS-9350 formulated as an injectable composition. 
     In one embodiment of the present invention, a compound of Formula I or Formula II is used in combination with SPI-452. In one embodiment, the combination is an oral fixed dose combination. In another embodiment, the compound of Formula I or Formula II is formulated as a long acting parenteral injection and SPI-452 is formulated as an oral composition. In one embodiment, there is provided a kit containing the compound of Formula I or Formula II formulated as a long acting parenteral injection and SPI-452 formulated as an oral composition. In another embodiment, the compound of Formula I or Formula II is formulated as a long acting parenteral injection and SPI-452 is formulated as an injectable composition. In one embodiment, there is provided a kit containing the compound of Formula I or Formula II formulated as a long acting parenteral injection and SPI-452 formulated as an injectable composition. 
     In one embodiment of the present invention, a compound of Formula I or Formula II is used in combination with compounds which are found in previously filed PCT/CN2011/0013021, which is herein incorporated by reference. 
     The above other therapeutic agents, when employed in combination with the chemical entities described herein, may be used, for example, in those amounts indicated in the Physicians&#39; Desk Reference (PDR) or as otherwise determined by one of ordinary skill in the art. 
     In another embodiment of the invention, there is provided a method for treating a viral infection in a mammal mediated at least in part by a virus in the retrovirus family of viruses which method comprises administering to a mammal, that has been diagnosed with said viral infection or is at risk of developing said viral infection, a compound of Formula I or Formula II. 
     In another embodiment of the invention, there is provided a method for treating a viral infection in a mammal mediated at least in part by a virus in the retrovirus family of viruses which method comprises administering to a mammal, that has been diagnosed with said viral infection or is at risk of developing said viral infection, a compound of Formula I or Formula II, wherein said virus is an HIV virus. In some embodiments, the HIV virus is the HIV-1 virus. 
     In another embodiment of the invention, there is provided a method for treating a viral infection in a mammal mediated at least in part by a virus in the retrovirus family of viruses which method comprises administering to a mammal, that has been diagnosed with said viral infection or is at risk of developing said viral infection, a compound of Formula I or Formula II, further comprising administration of a therapeutically effective amount of one or more agents active against an HIV virus. 
     In another embodiment of the invention, there is provided a method for treating a viral infection in a mammal mediated at least in part by a virus in the retrovirus family of viruses which method comprises administering to a mammal, that has been diagnosed with said viral infection or is at risk of developing said viral infection, a compound of Formula I or Formula II, further comprising administration of a therapeutically effective amount of one or more agents active against the HIV virus, wherein said agent active against HIV virus is selected from Nucleotide reverse transcriptase inhibitors; Non-nucleotide reverse transcriptase inhibitors; Protease inhibitors; Entry, attachment and fusion inhibitors; Integrase inhibitors; Maturation inhibitors; CXCR4 inhibitors; and CCR5 inhibitors. 
     In further embodiments, the compound of the present invention, or a pharmaceutically acceptable salt thereof, is chosen from the compounds set forth in Table 2. 
     
       
         
           
               
               
               
             
               
                 TABLE 2 
               
               
                   
               
               
                 Example 
                   
                   
               
               
                 No. 
                 Parent Structure 
                 Chemical Name 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-[(1R)-1- [(1R,2R,5R,10S,13R,14R, 17S,19R)-17-[(3- carboxy-3,3- dimethylpropanoyl)oxy]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-5-yl]-2-{[(4- chlorophenyl)methyl] amino}ethoxy]-2,2- dimethyl- 4-oxobutanoic acid 
               
               
                   
               
               
                 2 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-[(1R)-1- [(1R,2R,5R,10S,13R,14R, 17S,19R)-17-[(3- carboxy-3,3- dimethylpropanoyl)oxy]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-5-yl]-2-{[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}ethoxy]-2,2- dimethyl- 4-oxobutanoic acid 
               
               
                   
               
               
                 3 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-[(1S)-1- [(1R,2R,5R,10S,13R,14R, 17S,19R)-17-[(3- carboxy-3,3- dimethylpropanoyl)oxy]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-5-yl]-2-{[(4- chlorophenyl)methyl] amino}ethoxy]-2,2- dimethyl- 4-oxobutanoic acid 
               
               
                   
               
               
                 4 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4-[(1S)-1- [(1R,2R,5R,10S,13R,14R, 17S,19R)-17-[(3- carboxy-3,3- dimethylpropanoyl)oxy]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-5-yl]-2-{[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}ethoxy]- 2,2-dimethyl- 4-oxobutanoic acid 
               
               
                   
               
               
                 5 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(4- chlorophenyl)methyl] amino}ethyl)-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 6 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[2- (benzylamino)ethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 7 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(3- chlorophenyl)methyl] amino}ethyl)-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 8 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(3- chloro-2- fluorophenyl)methyl] amino}ethyl)-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 9 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[1-(4- chlorophenyl)cyclopropyl] amino}ethyl)- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 10 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}ethyl)-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 11 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[1-(4- chlorophenyl)cyclopropyl] [2- (dimethylamino)ethyl] amino}ethyl)-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 12 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(4- chlorophenyl)methyl] (methyl)amino}ethyl)- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 13 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{N-[(4- chlorophenyl)methyl] acetamido}ethyl)- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 14 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(4- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 15 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 16 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 17 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- (acetyloxy)-2-{[(4- chlorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 18 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 19 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 20 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- (acetyloxy)-2-{[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 21 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- (acetyloxy)-2-{[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 22 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 3,3-dimethyl-5- oxopentanoic acid 
               
               
                   
               
               
                 23 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 3,3-dimethyl-5- oxopentanoic acid 
               
               
                   
               
               
                 24 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(2- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 25 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(2- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 26 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- (acetyloxy)-2-{[(4- fluorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 27 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- (acetyloxy)-2-{[(4- fluorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 28 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-1-acetoxy-2-((4- fluorobenzyl)(methyl) amino)ethyl)-1-isopropyl- 5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 29 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl] (methyl)amino}-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 30 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-1-Acetoxy-2-((4- chlorobenzyl)(methyl) amino)ethyl)-1-isopropyl- 5a,5b,8,8,11a- penta methyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 31 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(4- chlorophenyl)methyl] (methyl)amino}-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 32 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-1-Acetoxy-2-(N-(4- chlorobenzyl)acetamido) ethyl)-1-isopropyl- 5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 32 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2-{N- [(4- chlorophenyl)methyl] acetamido}-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 33 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-(N-(4- Chlorobenzyl)acetamido)- 1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 34 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-2-(N-(4- Chlorobenzyl)acetamido)- 1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- penta methyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 35 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-(N-(2- Chlorobenzyl)acetamido)- 1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 36 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- (acetyloxy)-2-{[(2- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 37 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(3- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 38 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(3- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 37 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(3- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 38 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(3- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 39 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- (acetyloxy)-2-{[(3- chlorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 40 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- (acetyloxy)-2-{[(3- chlorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 41 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-1-acetoxy-2-((3- chlorobenzyl)(2- (dimethylamino)ethyl) amino)ethyl)-1-isopropyl- 5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 42 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-1-acetoxy-2-((3- chlorobenzyl)(2- (dimethylamino)ethyl) amino)ethyl)-1-isopropyl- 5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 43 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-2-((3- chlorobenzyl)(ethyl) amino)-1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- penta methyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 44 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-((3- Chlorobenzyl)(2- (dimethylamino)ethyl) amino)-1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 45 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)- 3a-((R)-2-((3- Chlorobenzyl)(2- (dimethylamino)ethyl) amino)-1-hydroxyethyl)- 1-isopropyl- 5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a, 8,9,10,11,11a,11b,12, 13,13a- octadecahydro-2H- cyclopenta[a]chrysen- 9-yl)oxy)-2,2-dimethyl- 4-oxobutanoic acid. 
               
               
                   
               
               
                 46 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2-{N- [(3- chlorophenyl)methyl] acetamido}-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 47 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2-{N- [(3- chlorophenyl)methyl] acetamido}-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 48 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-1-acetoxy-2-((2- chlorobenzyl)(2- (dimethylamino)ethyl) amino)ethyl)-1-isopropyl- 5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 49 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-2-((2- chlorobenzyl)(ethyl) amino)-1- hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 50 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(2- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 51 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(2- chlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 52 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- (acetyloxy)-2- (benzylamino)ethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 53 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- (acetyloxy)-2- (benzylamino)ethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 54 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-2-((3-Chloro-2- fluorobenzyl)amino)-1- hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 55 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-((3-chloro-2- fluorobenzyl)amino)-1- hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 56 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(3-chloro-2- fluorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 57 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(3-chloro-2- fluorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 58 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- hydroxy-2-[(propan-2- yl)amino]ethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 59 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- (cyclohexylamino)-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 53 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5S,10S,13R,14R, 17S,19R)-5-[1- hydroxy-2-(4- methylpiperazin-1- yl)ethyl]-1,2,14,18,18- pentamethyl-8-(propan- 2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 54 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(4- chlorophenyl)methyl] amino}ethyl)-1,2,14,18,18- pentamethyl-8-(propan- 2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 55 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5S,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-8-(propan- 2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 56 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5S,10S,13R,14R, 17S,19R)-5-[(1S)-2- [(cyclohexylmethyl)amino]- 1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-8-(propan- 2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 57 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- (cyclohexylamino)-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 58 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- hydroxy-2-(1,2,3,4- tetrahydroisoquinolin-2- yl)ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 59 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- hydroxy-2-(1,2,3,4- tetrahydroisoquinolin-2- yl)ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 60 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,10S,13R,14R, 17S,19R)-5-[(5S)-3-[1-(5- chloropyrimidin-2- yl)cyclopropyl]-2-oxo-1,3- oxazolidin-5-yl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 61 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,10S,13R,14R, 17S,19R)-5-[(5R)-3-[1-(5- chloropyrimidin-2- yl)cyclopropyl]-2-oxo-1,3- oxazolidin-5-yl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 62 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 3,3-dimethyl-5- oxopentanoic acid 
               
               
                   
               
               
                 63 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-2-(3,4- dihydroisoquinolin-2(1H)- yl)-1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 64 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-(3,4- dihydroisoquinolin-2(1H)- yl)-1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 65 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aS,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((R)-1-hydroxy-2-(4- methylpiperazin-1- yl)ethyl)-1-isopropyl- 5a,5b,8,8,11a- pentamethyl- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 66 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a-(2- ((4- chlorobenzyl)amino)ethyl)- 1-isopropyl- 5a,5b,8,8,11a- pentamethyl- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 67 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aS,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2- ((cyclohexylmethyl)amino)- 1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 68 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-(cyclohexylamino)- 1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 69 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-(benzyl(2- (dimethylamino)ethyl) amino)-1-hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a, octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 70 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-1- (acetyloxy)-2-{[2- (dimethylamino)ethyl][(4- fluorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 71 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-1- (acetyloxy)-2-{[2- (dimethylamino)ethyl][(4- fluorophenyl)methyl] amino}ethyl]-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 72 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(4- chlorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 3,3-dimethyl-5- oxopentanoic acid 
               
               
                   
               
               
                 73 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 5- (((3aR,5aR,5bR,7aR,9S, 11aR,11bR,13aS)-3a- ((S)-2-((4- chlorobenzyl)amino)-1- hydroxyethyl)-1- isopropyl-5a,5b,8,8,11a- pentamethyl-2-oxo- 3,3a,4,5,5a,5b,6,7,7a,8,9, 10,11,11a,11b,12,13,13a- octadecahydro-2H- cyclopenta[a]chrysen-9- yl)oxy)-3,3-dimethyl-5- oxopentanoic acid 
               
               
                   
               
               
                 74 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(2,4- dichlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 75 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {benzyl[2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 76 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[2- (dimethylamino)ethyl][(4- fluorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 77 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(4- fluorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 78 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[2- (dimethylamino)ethyl][(4- fluorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 79 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1S)-2- {[(4- fluorophenyl)methyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 80 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2- {[(2,4- dichlorophenyl)methyl][2- (dimethylamino)ethyl] amino}-1-hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 81 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-[(1R)-2-[1- (4-chlorophenyl)-N-[2- (dimethylamino)ethyl] formamido]-1- hydroxyethyl]- 1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 82 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)-5-(2-{[(2- chlorophenyl)methyl] amino}ethyl)-1,2,14,18,18- pentamethyl-7-oxo-8- (propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17-yl]oxy}- 2,2-dimethyl-4- oxobutanoic acid 
               
               
                   
               
               
                 83 
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 2,2-dimethyl-4-oxo-4- {[(1R,2R,5R,10S,13R,14R, 17S,19R)- 1,2,14,18,18- pentamethyl-7-oxo-5-[2- (phenylformamido)ethyl]- 8-(propan-2- yl)pentacyclo[11.8.0.0{circumflex over ( )}{2, 10}.0{circumflex over ( )}{5,9}.0{circumflex over ( )}{14,19}] henicos-8-en-17- yl]oxy}butanoic acid 
               
               
                   
               
            
           
         
       
     
     The compounds of Table 2 were synthesized according to the Synthetic Methods, General Schemes, and the Examples described below. 
     In certain embodiments, the compound(s) of the present invention, or a pharmaceutically acceptable salt thereof, is chosen from the compounds set forth in Table 2. 
     Synthetic Methods 
     The methods of synthesis for the provided chemical entities employ readily available starting materials using the following general methods and procedures. It will be appreciated that where typical or preferred process conditions (i.e., reaction temperatures, times, mole ratios of reactants, solvents, pressures, etc.) are given; other process conditions can also be used unless otherwise stated. Optimum reaction conditions may vary with the particular reactants or solvent used, but such conditions can be determined by one skilled in the art by routine optimization procedures. 
     Additionally, the methods of this invention may employ protecting groups which prevent certain functional groups from undergoing undesired reactions. Suitable protecting groups for various functional groups as well as suitable conditions for protecting and deprotecting particular functional groups are well known in the art. For example, numerous protecting groups are described in T. W. Greene and G. M. Wuts, Protecting Groups in Organic Synthesis, Third Edition, Wiley, N.Y., 1999, and references cited therein. 
     Furthermore, the provided chemical entities may contain one or more chiral centers and such compounds can be prepared or isolated as pure stereoisomers, i.e., as individual enantiomers or diastereomers, or as stereoisomer-enriched mixtures. All such stereoisomers (and enriched mixtures) are included within the scope of this specification, unless otherwise indicated. Pure stereoisomers (or enriched mixtures) may be prepared using, for example, optically active starting materials or stereoselective reagents well-known in the art. Alternatively, racemic mixtures of such compounds can be separated using, for example, chiral column chromatography, chiral resolving agents and the like. 
     The starting materials for the following reactions are generally known compounds or can be prepared by known procedures or obvious modifications thereof. For example, many of the starting materials are available from commercial suppliers such as Aldrich Chemical Co. (Milwaukee, Wis., USA), Bachem (Torrance, Ca., USA), Ernka-Chemce or Sigma (St. Louis, Mo., USA). Others may be prepared by procedures, or obvious modifications thereof, described in standard reference texts such as Fieser and Fieser&#39;s Reagents for Organic Synthesis, Volumes 1-15 (John Wiley and Sons, 1991), Rodd&#39;s Chemistry of Carbon Compounds, Volumes 1-5 and Supplementals (Elsevier Science Publishers, 1989), Organic Reactions, Volumes 1-40 (John Wiley and Sons, 1991), March&#39;s Advanced Organic Chemistry, (John Wiley and Sons, 4th Edition), and Larock&#39;s Comprehensive Organic Transformations (VCH Publishers Inc., 1989). 
     Unless specified to the contrary, the reactions described herein take place at atmospheric pressure, generally within a temperature range from −78° C. to 200° C. Further, except as employed in the Examples or as otherwise specified, reaction times and conditions are intended to be approximate, e.g., taking place at about atmospheric pressure within a temperature range of about −78° C. to about 110° C. over a period of about 1 to about 24 hours; reactions left to run overnight average a period of about 16 hours. 
     The terms “solvent,” “organic solvent,” and “inert solvent” each mean a solvent inert under the conditions of the reaction being described in conjunction therewith, including, for example, benzene, toluene, acetonitrile, tetrahydrofuranyl (“THF”), dimethylformamide (“DMF”), chloroform, methylene chloride (or dichloromethane), diethyl ether, methanol, N-methylpyrrolidone (“NMP”), pyridine and the like. 
     Isolation and purification of the chemical entities and intermediates described herein can be effected, if desired, by any suitable separation or purification procedure such as, for example, filtration, extraction, crystallization, column chromatography, thin-layer chromatography or thick-layer chromatography, or a combination of these procedures. Specific illustrations of suitable separation and isolation procedures can be had by reference to the examples herein below. However, other equivalent separation or isolation procedures can also be used. 
     When desired, the (R)- and (S)-isomers may be resolved by methods known to those skilled in the art, for example by formation of diastereoisomeric salts or complexes which may be separated, for example, by crystallization; via formation of diastereoisomeric derivatives which may be separated, for example, by crystallization, gas-liquid or liquid chromatography; selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic oxidation or reduction, followed by separation of the modified and unmodified enantiomers; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support, such as silica with a bound chiral ligand or in the presence of a chiral solvent. Alternatively, a specific enantiomer may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer to the other by asymmetric transformation. 
     EXAMPLES 
     The following examples serve to more fully describe the manner of making and using the above-described invention. It is understood that these examples in no way serve to limit the true scope of the invention, but rather are presented for illustrative purposes. In the examples below and the synthetic schemes above, the following abbreviations have the following meanings. If an abbreviation is not defined, it has its generally accepted meaning.
         aq.=aqueous   μL=microliters   μM=micromolar   NMR=nuclear magnetic resonance   boc=tert-butoxycarbonyl   br=broad   Cbz=benzyloxycarbonyl   d=doublet   δ=chemical shift   ° C.=degrees celsius   DCM=dichloromethane   dd=doublet of doublets   DMEM=Dulbeco&#39;s Modified Eagle&#39;s Medium   DMF=N,N-dimethylformamide   DMSO=dimethylsulfoxide   EtOAc=ethyl acetate   g=gram   h or hr=hours   HCV=hepatitis C virus   HPLC=high performance liquid chromatography   Hz=hertz   IU=International Units   IC 50 =inhibitory concentration at 50% inhibition   J=coupling constant (given in Hz unless otherwise indicated)   m=multiplet   M=molar   M+H + =parent mass spectrum peak plus H +     mg=milligram   min=minutes   mL=milliliter   mM=millimolar   mmol=millimole   MS=mass spectrum   nm=nanomolar   ppm=parts per million   q.s.=sufficient amount   s=singlet   RT=room temperature   sat.=saturated   t=triplet   TFA=trifluoroacetic acid       

     Equipment Description 
       1 H NMR spectra were recorded on a Bruker Avance-III 400 spectrometer. Chemical shifts are expressed in parts per million (ppm, 6 units). Coupling constants are in units of hertz (Hz). Splitting patterns describe apparent multiplicities and are designated as s (singlet), d (doublet), t (triplet), q (quartet), quint (quintet), m (multiplet), br (broad). 
     The analytical low-resolution mass spectra (MS) were recorded on Agilent 1200 HPLC/6110 or Agilent 1200 HPLC/6130 using a SunFire C18, 4.6×50 mm, 3.5 μm using a gradient elution method. 
     Solvent A: 0.01% trifluoroacetic acid (TFA) in water; 
     Solvent B: 0.01% TFA in acetonitrile; 
     Constant A for 1.2 min followed by 5%-95% or 20%-95% B over 4 min. 
     Schemes and Experimental Procedures 
     The following schemes and procedures illustrate how compounds of the present invention can be prepared. The specific solvents and reaction conditions referred to are also illustrative and are not intended to be limiting. Compounds not described are either commercially available or are readily prepared by one skilled in the art using available starting materials. The Examples disclosed herein are for illustrative purposes only and are not intended to limit the scope of the invention. All examples exhibited LHIV IC 50  values between 1 μM and 1 nM using the assay disclosed herein. 
     For several of the examples the stereochemistry of the C28 secondary alcohol when present was not definitively confirmed as to its absolute configuration. Unless stated otherwise, the compounds exemplified in the present application were isolated as optically pure stereoisomers and initially assigned to a configuration as drawn. There is the possibility that some of these may be listed as the opposite stereochemistry at that single C28 position as shown. This in no way is meant to limit the scope of the invention or utility of the compounds of Formula I. Additional examples contained within were determined to have the shown configuration by spectroscopic methods well known to those skilled in the art including, but not limited to, 1D and 2D NMR methods, vibrational circular dichroism and X-ray crystallography. These examples and the methods to make both diastereomers should serve to clearly exemplify the pure stereoisomers of both R and S configuration at the C28 position are readily obtained, separated and characterized and any remaining undefined examples could be readily confirmed by similar methods well known to one skilled in the art. 
     Synthesis of Aldehyde Intermediate 6. 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Step A: Intermediate 2 
     ((1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-9-Acetoxy-5a,5b,8,8,11a-pentamethyl-1-(prop-1-en-2-yl)icosahydro-1H-cyclopenta[a]chrysen-3a-yl)methyl acetate 
     To a solution of the intermediate 1 (20 g, 45.2 mmol), 4-dimethylaminopyridine (DMAP, 1.66 g, 13.6 mmol), and Et 3 N (63 mL, 136 mmol) in CH 2 Cl 2  (DCM, 100 mL) at room temperature was added acetic anhydride (Ac 2 O, 17.1 mL, 113 mmol). After it was heated at reflux overnight, and cooled down to room temperature, the reaction was quenched with water (50 mL). The organic phase was then washed with water (50 mL×2) and dried over sodium sulfate. After removing most of the organic solvent under reduced pressure, anhydrous ethanol (50 mL) was added and the resulting precipitates were collected by filtration as a white solid (intermediate 2, 20 g, 84%).  1 H NMR (400 MHz, CDCl 3 ) δ ppm 4.69 (1H, m), 4.59 (1H, m), 4.51-4.43 (1H, m), 4.25 (1H, d, J=11.2 Hz), 3.85 (1H, d, J=10.8 Hz), 2.49-2.40 (1H, m), 2.07 (3H, s), 2.04 (3H, s), 1.98-0.77 (42H, m). LC/MS: m/z calculated 526.4, found 527.7 (M+1)+. 
     Step B: Intermediate 3 
     ((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-acetoxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)methyl acetate 
     HBr in acetic acid (40 mL, 33%) was added to a suspension of the intermediate 2 (20 g, 38 mmol) in toluene (40 mL), Ac 2 O (40 mL), and acetic acid (AcOH, 40 mL) previously heated at 105° C. The reaction mixture was stirred and heated at this temperature for 1.5 h. After cooling down, sodium acetate (24 g) was added and the resulting reaction mixture was evaporated to dryness. The pale brownish residue was taken up in DCM (200 mL) and the organic phase was washed with water (100 mL×3), dried over sodium sulfate, and evaporated to dryness under reduced pressure to provide a residue, which was then recrystallized from ethanol (EtOH, 95%) and DCM, to afford the intermediate 3 (13.8 g, 69%) as a white solid.  1 H NMR (400 MHz, CDCl 3 ) δ ppm 4.50-4.46 (1H, m), 4.02 (1H, d, J=10.8 Hz), 3.98 (1H, d, J=10.8 Hz), 3.18-3.10 (1H, m), 2.43-2.40 (1H, m), 2.26-2.22 (2H, m), 2.04 (3H, s), 2.05 (3H, s), 2.00-1.95 (1H, m), 1.90-1.85 (1H, m), 1.77-0.83 (39H, m). LC/MS: m/z calculated 526.4, found 549.2 (M+Na)+. 
     Step C: Intermediate 4 
     ((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-Acetoxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)methyl acetate 
     A mixture of the intermediate 3 (7 g, 13.29 mmol), sodium acetate (NaOAc, 6.21 g, 76 mmol) and sodium dichromate dihydrate (4.75 g, 15.95 mmol) in anhydrous toluene (90 mL), AcOH (119 mL), and Ac 2 O (29 mL) was stirred at 60° C. overnight. After cooling down, the reaction mixture was partitioned between water (150 mL) and ethyl acetate (EtOAc, 250 mL). The organic phase was washed successively with: water (100 mL), a saturated solution of sodium carbonate (100 mL×2) and brine (100 mL×2), then dried over sodium sulfate, and concentrated under reduced pressure to afford a sticky oil. The sticky oil was triturated with MeOH (250 mL) and the precipitates were collected to give the intermediate 4 (6 g, 11.1 mmol, 83%) as a white solid.  1 H NMR (400 MHz, CDCl 3 ) δ ppm 4.52-4.46 (1H, m), 4.33 (1H, d, J=10.8 Hz), 4.06 (1H, d, J=11.2 Hz), 3.21-3.16 (1H, m), 2.86 (1H, dd, J=12.8, 3.2 Hz), 2.42-2.36 (1H, m), 2.05 (3H, s), 2.00 (3H, s), 1.94-0.84 (40H, m). LC/MS: m/z calculated 540.4, found 563.3 (M+Na)+. 
     Step D: Intermediate 5 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(Hydroxymethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     A mixture of the intermediate 4 (7 g, 12.94 mmol) and potassium hydroxide (KOH, 0.872 g, 15.5 mmol) in EtOH (200 mL) and toluene (200 mL) was stirred vigorously at room temperature for 1 h. The reaction mixture was neutralized with aqueous HCl (1N) to pH 7 and evaporated to dryness. The obtained residue was taken up in water and a small amount of acetone. The precipitates were collected and then washed with water and dried in vacuo to obtain the intermediate 5 (6.0 g, 93%) as a white solid. LC/MS: m/z calculated 498.4, found 499.3 (M+1)+. 
     Step E: Intermediate 6 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-Formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 5 (5.1 g, 10.23 mmol) in DCM (300 mL) at room temperature were added pyridinium chlorochromate (PCC, 6.61 g, 30.7 mmol), and silica gel (6.6 g). The reaction mixture was stirred at room temperature for 1 h. After the reaction was quenched with water, the organic phase was washed with saturated sodium bicarbonate solution (100 mL), dried over sodium sulfate, and evaporated under reduced pressure to provide a crude product, which was purified by column chromatography on silica gel (EtOAc:PE=1:10 to 1:5) to provide the intermediate 6 (4.2 g, 83%) as a white solid. LC/MS: m/z calculated 496.4, found 497.2 (M+1)+. 
     Synthesis of the oxo-butanoate intermediate 10 was accomplished according to the following procedures. 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 8 
     4-Ethoxy-2,2-dimethyl-4-oxobutanoic acid 
     A solution of 3,3-dimethyl-dihydrofuran-2,5-dione 7 (25 g, 195 mmol) in anhydrous EtOH (150 mL) was stirred at 50° C. overnight. After cooling down to room temperature, the solvent was removed under reduced pressure with a rotary evaporator and the residue was triturated with hexane at −50° C. to afford the intermediate 8 (25 g, 133 mmol, 67.9%) as a white solid.  1 H NMR (400 MHz, CDCl 3 ) δ ppm 4.13-4.18 (2H, q, J=7.2 Hz), 2.62 (2H, s), 1.28 (6H, s),1.32-1.25 (3H, t, J=7.6 Hz). LC/MS: m/z calculated 174.1, found 173.1 (M−1)−. 
     Step B: Intermediate 9 
     1-Tert-butyl 4-ethyl 2,2-dimethylsuccinate 
     To a mixture of the intermediate 8 (20 g, 109 mmol), magnesium sulfate (52.5 g, 436 mmol), and tert-butanol (60 mL) in DCM (480 mL) was added to sulfuric acid (8.72 mL, 164 mmol). After stirring at room temperature overnight, the reaction mixture was poured into saturated sodium bicarbonate solution (300 mL) and water (300 mL). DCM was added to extract the desired product, and the organic phase was washed with brine, dried, and concentrated to afford the intermediate 9 (19 g, 83 mmol, 80%) as a colorless oil.  1 H NMR (400 MHz, CDCl 3 ) δ ppm 4.02-4.08 (2H, q, J=7.2 Hz), 2.46 (2H, s), 1.07 (9H, s), 1.14-1.20 (9H, m). LC/MS: m/z calculated 230.2, found 253.1 (M+Na)+. 
     Step C: Intermediate 10 
     4-(Tert-butoxy)-3,3-dimethyl-4-oxobutanoic acid 
     To a solution of the intermediate 9 (10 g, 41.3 mmol) in EtOH (200 mL) was added to potassium hydroxide (12.86 g, 206 mmol) in water (100 mL) at room temperature. The reaction mixture was stirred at room temperature for 2 h. The pH of the reaction mixture was adjusted to 3-4 by 1N HCl. The resulting solution was extracted with ether (300 mL), and the ether phase was dried and concentrated to afford a crude product, which was re-crystallized from hexane at −10° C. to afford the intermediate 10 (4 g, 19.78 mmol, 47.9%) as a white solid.  1 H NMR (400 MHz, CDCl 3 ) δ ppm 2.58 (2H, s), 1.43 (9H, s), 1.25 (6H, s). LC/MS: m/z calculated 202.1, found 201.1 (M−1) − . 
     Synthesis of diastereomeric intermediates 18 and 19. 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Step A: Intermediate 11 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(1-Hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a mixture of (3b)-21,28-dioxolup-18-en-3-yl acetate (6) (25 g, 50.3 mmol)and nitromethane (150 ml, 2782 mmol) stirred at room temp was added to triethylamine (75 ml, 538 mmol) in one charge. The reaction mixture was stirred at rt overnight. The reaction mixture was concentrated and washed with petroleum ether/EtOAc (2:1, 100 mL) to give the product of (24 g, 43.0 mmol, 85% yield) as a white solid. LC/MS: m/z calculated 557.4, found 558.2 (M+1) + . 
     Step B: Intermediate 12 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-Amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of 11 (15.0 g, 26.9 mmol) in methanol (300 mL) was added NiCl 2 .6H 2 O (9.59 g, 40.3 mmol) at −5° C. and was slowly added sodium borohydride (10.17 g, 269 mmol) at 5˜10° C. (internal temp). The reaction mixture was stirred at −5° C. (bath temp) for 30 min. The mixture was then quenched with saturated NH 4 Cl (200 mL), diluted with EtOAc (1500 mL), then stirred at rt overnight. And the organic layer was washed with saturated NH 4 Cl (50 mL), water (800 mL), brine (800 mL) and was dried over MgSO 4 , filtered and concentrated to afford a solid, which was washed with petroleum ether to give the 12 (14 g, 24.40 mmol, 91% yield) as a white solid. LC/MS: m/z calculated 527.4, found 528.3 (M+1) + . 
     Step C: Intermediate 13 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of 12 (8 g, 14.58 mmol), triethylamine (0.813 ml, 5.83 mmol) and MgSO 4  (2.63 g, 21.87 mmol) in methanol (225 ml) stirred at rt was added 4-chlorobenzaldehyde (2.056 ml, 17.50 mmol). The reaction mixture was stirred at rt for 2 h, then cooled to −5° C. and NaBH 4  was added in small portions during 10 min and stirred for 30 min. Following this, Boc 2 O (4.06 ml, 17.50 mmol) was added. The reaction was warmed to rt. and stirred for 1 h. The reaction mixture was poured into ice water (300 ml) and the solids were collected and dried to give 13 (7 g, 8.56 mmol, 58.7% yield) as a white foam. LC/MS: m/z calculated 751.5, found 774.3 (M+Na) + . 
     Step D: Intermediate 14 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a mixture of 13 (25 g, 29.9 mmol) in dichloromethane (200 mL), was added PCC (50 g, 232 mmol) and silica gel 50 g, This mixture was stirred at rt overnight. The solids were removed by filtration to obtain black solution, which was concentrated and purified by silica gel column chromatography eluting with hex/EtOAc (10:1) to afford the 14 (20 g, 23.99 mmol, 80% yield) as a white solid. LC/MS: m/z calculated 749.4, found 772.2 (M+Na) + . 
     Step E: Intermediate 15 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)amino)acetyl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-2-one 
     To a solution of 14 in 1,4-dioxane (200 mL) and methanol (200 mL) stirred at rt was added con HCl (100 mL) in one charge. The reaction mixture was stirred overnight at 45° C. The reaction mixture was concentrated and washed with acetone to give 15 (12.5 g, 17.45 mmol, 87% yield) as a white foam. LC/MS: m/z calculated 607.38, found 608.0 (M+1) + . 
     Step F: Intermediate 16 
     Tert-butyl 4-chlorobenzyl(2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-oxoethyl)carbamate 
     To a solution of 15 (6 g, 9.86 mmol) and Et 3 N (5.50 mL, 39.5 mmol) in dichloromethane (30 mL) stirred at rt was added Boc 2 O (2.290 mL, 9.86 mmol) in one charge. The reaction mixture was stirred at rt for 2 h. From TLC, the reaction was finished. The solvent was removed in vacuo. The crude product 16 (6 g, 8.47 mmol, 86% yield) was used without further purification. 
     Step G: Intermediate 17 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate 
     To a solution of 10 (5.14 g, 25.4 mmol), DMAP (5.17 g, 42.3 mmol) and 16 (6 g, 8.47 mmol) in dichloromethane (50 mL) stirred at rt was added EDC (8.12 g, 42.3 mmol). The reaction mixture was stirred at rt until starting material disappeared as monitored by TLC. After the reaction was finished, the mixture was diluted with CH 2 Cl 2 , and washed with aq NH 4 Cl. The organic layer was dried with Na 2 SO 4  and the solvent was removed in vacuo. The material was purified by silica gel chromatography (hex:EtOAc, 12:1 to 5:1) to give 17 (5.4 g, 6.05 mmol, 71.4% yield) as a light white solid. The proton NMR consists of a mixture of rotomers.  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.34-7.24 (m, 2H), 7.23-7.08 (m, 2H), 4.63-4.29 (m, 3H), 4.11-3.39 (m, 2H), 3.28-3.06 (m, 1H), 2.69-0.69 (m, 68H). 
     Step H: Intermediates 18 and 19 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(Tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate (18) &amp; 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate (19) 
     To a solution of and 17 (1.5 g, 1.680 mmol) in methanol (10 mL)and THF (10.00 mL) stirred at 0° C. was added NaBH 4  (0.127 g, 3.36 mmol). The reaction mixture was stirred at 0° C. until starting material disappeared (about 2 hours) as monitored by TLC. Upon completion the mixture was diluted with water, extracted with EtOAc and the organic layer was dried with Na 2 SO 4 . The residue was purified by silica gel chromatography (hex:EtOAc, 7:1 to 5:1) to give the diastereomer with the S configuration 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (18) (700 mg, 0.782 mmol, 46.6% yield) and the diastereomer with the R configuration 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (19) (500 mg, 0.559 mmol, 33.3% yield) both as a light white solids. For 18  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.40-7.24 (m, 2H), 7.18 (d, J=8.3 Hz, 2H), 4.87-4.57 (m, 1H), 4.51 (dd, J=5.3, 11.0 Hz, 1H), 4.42-4.13 (m, 2H), 3.43-3.05 (m, 3H), 2.97-2.78 (m, 1H), 2.62-2.47 (m, 2H), 2.47-2.29 (m, 1H), 2.01-0.70 (m, 64H); LC/MS: m/z calculated 893.6, found 916.5 (M+Na) + . For 19  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.36-7.23 (m, 2H), 7.12 (d, J=8.3 Hz, 2H), 4.56-4.14 (m, 4H), 3.45-3.18 (m, 1H), 3.15-2.97 (m, 1H), 2.76-0.71 (m, 69H); LC/MS: m/z calculated 893.6, found 916.7 (M+Na) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 1 
     Compound 21 
     4-((R)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((3-carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((4-chlorobenzyl)amino)ethoxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 20 
     4-((R)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9((4-(tert-butoxy)-3,3-dimethyl-4-oxobutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysTn-3a-yl)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)1-tert-butyl 2,2-dimethylsuccinate 
     To a solution of 10 (543 mg, 2.68 mmol), DMAP (205 mg, 1.677 mmol) in dichloromethane (5 mL) stirred at rt was added EDC (514 mg, 2.68 mmol). The reaction mixture was stirred at rt for 1 h. Then 19 (300 mg, 0.335 mmol) was added to the reaction mixture. Upon completion, the mixture was washed with 2 M HCl, water and brine. The organic layer was dried over Na 2 SO 4  and concentrated to give the crude product 20 (400 mg, 0.278 mmol, 83% yield) as an oil. This material was used in the next step without further purification. 
     Step B: Compound 21 
     4-((R)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((3-Carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((4-chlorobenzyl)amino)ethoxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 20 (400 mg, 0.371 mmol) in dichloromethane (5 mL) stirred at rt was added TFA (2 mL, 26.0 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was evaporated to afford the the TFA salt of intermediate 21 (350 mg, 0.343 mmol, 93% yield) as a light oil.  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.33-7.12 (m, 4H), 5.76-5.60 (m, 1H), 4.48-4.32 (m, 1H), 4.26-3.98 (m, 2H), 3.18-2.89 (m, 2H), 2.89-2.68 (m, 2H), 2.69-0.60 (m, 58H); LC/MS: m/z calculated 865.5, found 866.3 (M+1) + . 
     Example 2 
     Compound 22 
     4-((R)-1-((3aR,5aR,5b R,7aR,9S,11aR,11bR,13aS)-9-((3-Carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethoxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 2-(dimethylamino)acetaldehyde, hydrochloride (441 mg, 3.57 mmol) in methanol (12 mL) was added 21, (as it&#39;s trifluoroacetic acid salt) (350 mg, 0.357 mmol). The mixture&#39;s pH was adjusted to 7-8 with Et 3 N. The reaction mixture was stirred at rt for 2 h then NaBH 3 CN (224 mg, 3.57 mmol) was added and mixture was stirred overnight. The reaction was filtered and evaporated to afford a crude product. The residue was purified by preparative-HPLC to obtain compound 22 as a TFA salt (200 mg, 0.168 mmol, 47.2% yield) as a white solid.  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.33 (d, J=8.3 Hz, 2H), 7.22 (d, J=8.3 Hz, 2H), 5.83-5.67 (m, 1H), 4.54-4.42 (m, 1H), 3.88-3.75 (m, 1H), 3.65-3.49 (m, 1H), 3.04-0.60 (m, 71H); LC/MS: m/z calculated 936.6, found 937.4 (M+1) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 3 
     Compound 25 
     4-((S)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((3-Carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethoxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 23 
     4-((S)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((4-(Tert-butoxy)-3,3-dimethyl-4-oxobutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)1-tert-butyl 2,2-dimethylsuccinate 
     To a solution of 10 (203 mg, 1.006 mmol), DMAP (123 mg, 1.006 mmol) in dichloromethane (10 ml) stirred at rt was added EDC (321 mg, 1.677 mmol). The reaction mixture was stirred at rt for 1 h, then 18 (300 mg, 0.335 mmol) was added to the reaction. Upon completion, silica gel was added, the mixture was concentrated and purified by chromatography (petroleum ether:EtOAc 6:1 to 3:1) to give the 23 (344 mg, 0.319 mmol, 95% yield) as a light white solid. 
     Step B: Compound 24 
     4-((S)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((4-(Tert-butoxy)-3,3-dimethyl-4-oxobutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)1-tert-butyl 2,2-dimethylsuccinate 
     
       
         
         
             
             
         
       
     
     To a solution of 23 (115 mg, 0.107 mmol) in DCM (5 mL) was added TFA (2.5 mL, 0.107 mmol). The reaction mixture was stirred at rt for 2 h and evaporated in vacuo to afford crude product. This material was then washed with satd. sodium bicarbonate solution, water and brine. The organics were filtered and concentrated to give the 24 (89 mg, 0.023 mmol, 21.17% yield) as a white solid.  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.40 (s, 4H), 5.97-5.65 (m, 1H), 4.93-3.75 (m, 3H), 3.59-3.27 (m, 1H), 3.27-2.92 (m, 3H), 2.83-2.11 (m, 5H), 1.98-0.59 (m, 52H) LC/MS: m/z calculated 865.5, found 866.4 (M+1) + . 
     Example 4 
     Compound 25 
     4-((S)-1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((3-Carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethoxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 2-(dimethylamino)acetaldehyde (325 mg, 2.63 mmol) in methanol (5 ml) and DCE (50 ml) was added 24 (228 mg, 0.263 mmol) and triethylamine (0.110 ml, 0.789 mmol). The reaction mixture was stirred at rt for 3 h. The reaction was cooled to 0° C., and sodium cyanoborohydride (24.80 mg, 0.395 mmol) was added and resultant mixture was stirred overnight. The reaction was filtered and the filter cake was washed with DCM. The filtrate was concentrated under reduced pressure and the residue was purified by preparative-HPLC to give the title compound 25 as a TFA salt (76 mg, 0.065 mmol, 24.78% yield) as a white solid.  1 H NMR (400 MHz, CHLOROFORM-d) δ 7.38-7.30 (m, 2H), 7.30-7.19 (m, 2H), 5.82-5.69 (m, 1H), 4.53-4.44 (m, 1H), 4.05-3.89 (m, 1H), 3.70-3.54 (m, 1H), 3.41-2.51 (m, 15H), 2.45-2.12 (m, 2H), 1.97-0.65 (m, 54H); LC/MS: m/z calculated 936.6, found 937.4 (M+1) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 5 
     Compound 32 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 26 
     1-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-Acetoxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-nitroethyl acetate 
     To a solution of 11 (20 g, 35.9 mmol) in acetic anhydride (100 mL, 1065 mmol) was added 4-methylbenzenesulfonic acid (1.852 g, 10.76 mmol). The reaction mixture was stirred at rt overnight. EtOAc (50 ml) and NaHCO 3  aq. (100 ml) were added. The organic layer was separated, washed with water, brine,and dried (Na 2 SO 4 ). Removal of the solvent resulted in the crude product which was used directly in the next step. LC/MS: m/z calculated 599.4, found 600.3 (M+1) + . 
     Step B: Intermediate 27 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-Isopropyl-5a,5b,8,8,11a-pentamethyl-3a-(2-nitroethyl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of 26 (21 g, 35.0 mmol) in THF (30 mL) and EtOH (80 mL) in an ice bath was added NaBH 4  (5.30 g, 140 mmol). The mixture was then kept at rt for about 1 h, diluted with ethyl acetate (200 mL) and extracted with aqueous 10% citric acid solution (150 mL), saturated aqueous NaHCO 3  (150 mL), and brine (150 mL). The organic layer was dried (Na 2 SO 4 ) and evaporated to give the crude product 27 (19 g, 27.2 mmol, 78% yield). This was used directly for the next step. LC/MS: m/z calculated 541.4, found 542.3 (M+1) + . 
     Step C: Intermediate 28 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-Isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-oxoethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a stirred solution of 27 (10 g, 18.46 mmol) in acetone (90.00 mL) and methanol (90 mL) at −5° C. was added dropwise a solution of KOH (1.036 g, 18.46 mmol) in 5 mL water, then followed by KMnO4 (1.896 g, 12.00 mmol) and MgSO 4  (1.666 g, 13.84 mmol) in 90 mL of water. The temperature was held below 0° C. for 1 h and for another 1 h at room temperature. The reaction mixture was diluted with DCM (100 mL), then filtered on Celite™ and washed with DCM. The organic phase was concentrated, then extracted with DCM, to give the crude product 28 (5 g, 8.12 mmol, 44.0% yield) which was used directly for the next step without further purification.  1 H NMR (400 MHz, CHLOROFORM-d) δ 9.64 (s, 1H), 4.49 (dd, J=5.5, 11.0 Hz, 1H), 3.27-3.06 (m, 1H), 2.87-2.76 (m, 1H), 2.76-2.64 (m, 1H), 2.64-2.54 (m, 1H), 2.45 (d, J=18.8 Hz, 1H), 2.20 (d, J=18.8 Hz, 1H), 2.05 (s, 3H), 1.99-0.67 (m, 39H); LC/MS: m/z calculated 510.4, found 533.3 (M+Na) + . 
     Step D: Intermediate 29 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of 28 (1.5 g, 2.94 mmol), TEA (0.164 ml, 1.175 mmol) and MgSO 4  (0.530 g, 4.41 mmol) in methanol (15 ml) stirred at rt was added 4-chlorobenzylamine (0.487 ml, 4.11 mmol). The reaction mixture was stirred at 20° C. for 2 h, then cooled to −5° C. and NaBH 4  (0.167 g, 4.41 mmol) was added in small portions during 10 min and stirred for 30 min. BOC 2 O (0.750 ml, 3.23 mmol) was then added. The reaction was warmed to rt and stirred for 1 h. The reaction mixture was poured into ice water (300 ml) and solids were collected and dried to give 29 (1.8 g, 2.106 mmol, 72%) as a white foam. This material was used without further purification. LC/MS: m/z calculated 735.5, found 758.3 (M+Na) + . 
     Step E: Intermediate 30 
     Tert-butyl 4-chlorobenzyl(2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)ethyl)carbamate 
     To a solution of 29 (1.6 g, 2.173 mmol) in methanol (6 mL) and THF (6 mL), and water (6 mL) was added NaOH (5.21 g, 130 mmol). The reaction mixture was stirred at rt for 1 h. Water and EtOAc were added upon completion and the layers separated. The organic layer was washed with water, brine, and dried (Na 2 SO 4 ). Removal of the solvent gave 30 (1.4 g, 1.590 mmol, 73%) as a white solid. LC/MS: m/z calculated 693.5, found 716.3 (M+Na) + . 
     Step F: Intermediate 31 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate 
     To a solution of DMAP (0.862 g, 7.06 mmol), EDC (1.932 g, 10.08 mmol) and 4-tert-butoxy-3,3-dimethyl-4-oxobutanoic acid (10) (1.631 g, 8.06 mmol) in DCM (60 mL) stirred at 20° C. for 30 min was added 30 (1.4 g, 2.016 mmol). The reaction mixture was stirred at 20° C. for 1 h. The mixture was washed with saturated ammonium chloride, water, and saturated NaHCO 3 , water, and lastly brine. The organic layer was dried over Na 2 SO 4 , filtered and concentrated to give the crude product, which was purified by silica gel column eluting with petrol ether/Ethyl acetate (4:1) to afford the product 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (1.4 g, 0.986 mmol, 49%) as a yellow solid. 
     Step G: Compound 32 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 31 (1.4 g, 1.593 mmol) in DCM (6 mL) was added TFA (3 mL). The reaction mixture was stirred at 20° C. for 2 h and evaporated in vacuo to afford crude product which was purified by preparative-HPLC to give the title compound trifluoroacetic acid salt (1 g, 73%) as a white solid. 1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.57-7.42 (m, 4H), 4.51 (dd, J=5.1, 11.2 Hz, 1H), 4.29-4.15 (m, 2H), 3.30-3.18 (m, 1H), 2.98-2.69 (m, 3H), 2.62 (q, J=16.0 Hz, 2H), 2.29 (d, J=19.1 Hz, 1H), 2.17-0.79 (m, 48H); LC/MS: m/z calculated 721.5, found 722.3 (M+1) + . 
     Example 6 
     Compound 33 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(Benzylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 5 as a TFA salt.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.48 (s, 5H), 4.51 (dd, J=5.1, 11.2 Hz, 1H), 4.30-4.17 (m, 2H), 3.31-3.19 (m, 1H), 2.96-2.71 (m, 3H), 2.62 (q, J=15.9 Hz, 2H), 2.29 (d, J=18.8 Hz, 1H), 2.21-0.78 (m, 48H); LC/MS: m/z calculated 687.5, found 688.4 (M+1) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 7 
     Compound 39 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((3-Chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Compound 34 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-Aminoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-(2-nitroethyl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (27) (10 g, 18.46 mmol) and nickel(II) chloride (7.18 g, 55.4 mmol) in methanol (100 ml) was added sodium borohydride (6.98 g, 185 mmol) portionwise during 1 hour. The reaction mixture was stirred at 0° C. for 1 h. The mixture was diluted with EtOAc (250 mL). The organic phase was washed with sat. NH 4 Cl (50 mL) until the organic layer change to colorless. The combined organic layers were dried over anhydrous Na 2 SO 4 , and concentrated in vacuo to get the crude product (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-aminoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (34) (7 g, 12.63 mmol, 68.4% yield), which was used without further purification.  1 H NMR (400 MHz, CHLOROFORM-d) δ=9.15 (dd, J=4.9, 11.4 Hz, 1H), 8.12 (br. s., 2H), 7.97-7.81 (m, 1H), 7.63-7.49 (m, 1H), 7.26-7.15 (m, 1H), 7.15-7.04 (m, 1H), 6.98 (d, J=18.6 Hz, 1H), 6.81-5.38 (m, 47H); LC/MS: m/z calculated 511.4, found 512.3 (M+1) + . 
     Step B: Compound 35 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-aminoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (34) (5.3 g, 10.36 mmol), TEA (2.165 mL, 15.53 mmol) and TEA (2.165 mL, 15.53 mmol) in dichloromethane (DCM) (100 mL) stirred at room temp was added BOC 2 O (2.404 mL, 10.36 mmol). The reaction mixture was stirred at 20° C. for 2 h. The reaction mixture was poured into ice water (300 ml) and the solids were collected and dried to give (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (35) (5.8 g, 8.34 mmol, 81% yield) as a white foam. This was used crude in next reaction without further purification.  1 H NMR (400 MHz, CHLOROFORM-d) δ=4.58-4.37 (m, 2H), 3.26-2.96 (m, 4H), 2.93-2.72 (m, 2H), 2.28 (d, J=18.8 Hz, 1H), 2.11-0.71 (m, 53H); LC/MS: m/z calculated 611.5, found 634.3 (M+Na) + . 
     Step C: Compound 36 
     Tert-butyl(2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)ethyl)carbamate 
     A solution of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (35) (5.8 g, 9.48 mmol), NaOH (11.37 g, 284 mmol) in methanol (4 mL), THF (6 mL), and Water (2 mL) was stirred at room temperature for 1 h. EtOAc was added and the reaction mixture was washed with water. The organic layer was separated, washed with water and brine,and dried (Na 2 SO 4 ). Removal of the solvent provide tert-butyl (2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)ethyl)carbamate (36) (5.4 g, 7.87 mmol, 83% yield) as a white solid.  1 H NMR (400 MHz, CHLOROFORM-d) δ=4.53-4.36 (m, 1H), 3.30-2.94 (m, 4H), 2.94-2.67 (m, 2H), 2.28 (d, J=18.8 Hz, 1H), 2.11-0.55 (m, 50H); LC/MS: m/z calculated 569.4, found 592.3 (M+Na) + . 
     Step D: Compound 37 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate 
     A solution of 4-tert-butoxy-3,3-dimethyl-4-oxobutanoic acid (10) (4.79 g, 23.69 mmol), DMAP (3.47 g, 28.4 mmol) and tert-butyl (2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)ethyl)carbamate (36) (5.4 g, 9.48 mmol) EDC (9.08 g, 47.4 mmol) in dichloromethane (DCM) (25 mL) was stirred at rt overnight. NH 4 Cl was added and the mixture was extracted with DCM, and purified by chromatography with PE:EA (10:1) to give the 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (37) (6.7 g, 8.75 mmol, 92% yield).  1 H NMR (400 MHz, CHLOROFORM-d) δ=4.62-4.39 (m, 2H), 3.24-3.10 (m, 1H), 3.10-2.93 (m, 1H), 2.93-2.68 (m, 2H), 2.62-2.49 (m, 1H), 2.28 (d, J=18.8 Hz, 1H), 2.11-0.69 (m, 66H); LC/MS: m/z calculated 753.6, found 776.5 (M+Na) + . 
     Step E: Compound 38 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-Aminoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (37) (6.7 g, 8.88 mmol) in DCM (20 mL) stirred at rt was added TFA (4 ml, 51.9 mmol). The reaction mixture was stirred at rt for 2 h. The reaction mixture was washed with sat. NaHCO 3 . A solid was formed and collected by filtration to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-aminoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (38) (4 g, 6.55 mmol, 73.7% yield) as a white solid. 1 H NMR (400 MHz, DMSO-d 6 ) δ=4.44-4.28 (m, 1H), 3.20-3.05 (m, 1H), 2.87-2.75 (m, 1H), 2.46-2.16 (m, 6H), 1.97-0.66 (m, 47H); LC/MS: m/z calculated 597.4, found 598.3 (M+1) + . 
     Step F: Compound 39 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((3-Chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-aminoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (38) (300 mg, 0.502 mmol), TEA (0.028 ml, 0.201 mmol) and MgSO 4  (91 mg, 0.753 mmol) in methanol (15 ml) stirred at room temp was added 3-chlorobenzaldehyde (85 mg, 0.602 mmol). The reaction mixture was stirred at 20° C. for 2 h. The reaction mixture was then cooled to 0° C. and NaBH 4  (1.541 g, 40.7 mmol) was added in portions within 30 min, after which the solution was allowed to stirred for 1 h. The mixture was purified by preparative-TLC eluting with DCM/MeOH (20:1) to give 39 (108 mg, 0.147 mmol, 29%).  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.41 (s, 1H), 7.38-7.24 (m, 3H), 4.48 (dd, J=5.7, 10.7 Hz, 1H), 3.89-3.67 (m, 2H), 3.26-3.11 (m, 1H), 2.84-2.73 (m, 1H), 2.66-2.47 (m, 2H), 2.46-2.34 (m, 1H), 2.34-2.21 (m, 2H), 2.08-0.77 (m, 48H). 
     Example 8 
     Compound 40 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((3-Chloro-2-fluorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 7, but as a TFA salt.  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.46-7.38 (m, 1H), 7.35 (t, J=6.6 Hz, 1H), 7.17 (t, J=7.7 Hz, 1H), 4.48 (dd, J=5.5, 10.6 Hz, 1H), 3.97-3.72 (m, 2H), 3.27-3.12 (m, 1H), 2.85-2.73 (m, 1H), 2.67-2.45 (m, 2H), 2.44-2.16 (m, 3H), 2.09-0.73 (m, 48H). 
     Example 9 
     Compound 41 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((1-(4-chlorophenyl)cyclopropyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 5, but as a TFA salt. The final step was performed according to the following procedure. To a solution of 4-(tert-butoxy)-3,3-dimethyl-4-oxobutanoic acid (10) (0.988 g, 4.89 mmol), EDC (1.171 g, 6.11 mmol) and DMAP (0.522 g, 4.28 mmol) in DCM (12 mL) stirred at room temp was added tert-butyl (1-(4-chlorophenyl)cyclopropyl)(2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)ethyl)carbamate (1.1 g, 1.221 mmol). The reaction mixture was stirred at rt for 2 h. Next, TFA (6 mL) was added to the reaction mixture. Then, the reaction solution was stirred at rt for another 1.5 h, and the product was extracted with DCM (60 mL*3), and the combined organic phase was washed with brine (100 ml), dried over Na 2 SO 4 , concentrated and the residue was purified by recrystallization from DCM and hexane (1:6) resulting in 800 mg of the TFA salt of 41 as white solid.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.64-7.44 (m, 4H), 4.50 (dd, J=5.1, 11.2 Hz, 1H), 3.26-3.10 (m, 1H), 2.84-2.41 (m, 5H), 2.24-2.12 (m, 1H), 2.12-0.78 (m, 52H); LC/MS: m/z calculated 747.5, found 748.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 10 
     Compound 42 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 2-(dimethylamino)acetaldehyde (244 mg, 1.977 mmol) in methanol (100 ml) was added 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, hydrochloride (32) (300 mg, 0.395 mmol). The reaction mixture was stirred at 40° C. for 2 h. The reaction was cooled to rt and sodium cyanoborohydride (24.84 mg, 0.395 mmol) was added and the resultant solution was stirred overnight. The reaction was diluted with ammonium chloride (40 ml), and extracted with DCM (60 ml×3). The combined organic layers were washed with brine (20 ml), dried over sodium sulfate, filtered and concentrated in vacuo. The residue was purified by preparative-HPLC to afford 42 as a trifluoroacetic acid salt (200 mg, 49% yield) as a white solid.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.52-7.34 (m, 4H), 4.50 (dd, J=5.0, 11.0 Hz, 1H), 4.07-3.88 (m, 1H), 3.83-3.62 (m, 1H), 3.53-3.35 (m, 2H), 3.22-2.89 (m, 9H), 2.71-2.54 (m, 2H), 2.50-2.21 (m, 4H), 2.06-0.83 (m, 48H); LC/MS: m/z calculated 792.5, found 793.4 (M+1) + . 
     Example 11 
     Compound 43 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((1-(4-Chlorophenyl)cyclopropyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 10, as a TFA salt.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.35 (s, 4H), 4.51 (dd, J=5.1, 11.2 Hz, 1H), 3.31-3.17 (m, 2H), 3.12-2.73 (m, 9H), 2.73-2.51 (m, 2H), 2.44-2.17 (m, 3H), 2.14-0.77 (m, 53H); LC/MS: m/z calculated 818.4, found 819.4 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 12 
     Compound 44 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 32 (150 mg, 0.208 mmol) in MeOH (6 mL) at rt was added formaldehyde (33.7 mg, 0.415 mmol). The reaction mixture was stirred at rt for 2 h. NaCNBH 4  (104 mg, 1.661 mmol) was added portionwise and the mixture stirred for overnight. The solvent was evaporated, and the mixture was purified by preparative HPLC to provide the TFA salt of 44 (60 mg, 34%).  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.60-7.44 (m, 4H), 4.56-4.37 (m, 2H), 4.36-4.24 (m, 1H), 3.28-3.14 (m, 1H), 2.89 (m, 5H), 2.73-2.50 (m, 2H), 2.28 (d, J=19.1 Hz, 1H), 2.20-0.83 (m, 49H); LC/MS: m/z calculated 735.5, found 736.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 13 
     Compound 45 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(4-Chlorobenzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     A mixture of 32 (150 mg, 0.208 mmol), TEA (210 mg, 2.076 mmol) and DMAP (5 mg, 0.042 mmol) in DCM (5 ml) was stirred for 3 hours. Next, the mixture was quenched with water (50 ml). The organics were then washed with water (2×50 ml), dried over Na 2 SO 4 , and evaporated in vacuo to afford crude product. This was then purified by preparative HPLC to give 45 (65 mg, 0.085 mmol, 41%) as a white solid. Mixture of rotomers.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.49-7.18 (m, 4H), 4.80-4.42 (m, 3H), 3.27-2.81 (m, 4H), 2.76-2.52 (m, 2H), 2.36-2.10 (m, 4H), 2.08-0.82 (m, 48H); LC/MS: m/z calculated 763.5, found 764.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 14 
     Compound 46 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (19) (300 mg, 0.335 mmol) in DCM (30 mL) stirred at rt was was added TFA (15 mL, 195 mmol). The reaction mixture was stirred at rt until LCMS and TLC indicated SM disappeared. The solvent was removed in vacuo and the residue was purified by preparative HPLC to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (46) (100 mg, 0.135 mmol, 40.4% yield) as a TFA salt.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.38-7.18 (m, 4H), 4.54-4.42 (m, 1H), 4.28-4.14 (m, 1H), 3.93-3.70 (m, 2H), 3.19-3.00 (m, 1H), 2.75-0.69 (m, 52H); LC/MS: m/z calculated 737.4, found 738.2 (M+1) + . 
     Example 15 
     Compound 47 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (18) (130 mg, 0.145 mmol) in DCM (30 mL) stirred at rt was added TFA (15 mL, 195 mmol). The reaction mixture was stirred at rt until LCMS and TLC indicated starting material disappeared. The solvent was evaporated and then then CH 3 CN and H 2 O was added and the material was lyophilized to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (47), trifluoroacetic acid salt (120 mg, 0.139 mmol, 96% yield).  1 H NMR (400 MHz, CHLOROFORM-d) δ=10.30 (br. s., 1H), 8.08 (br. s., 1H), 7.38 (s, 4H), 4.73-4.33 (m, 2H), 4.28-4.07 (m, 2H), 3.34-3.06 (m, 2H), 2.99-2.74 (m, 2H), 2.73-2.50 (m, 2H), 2.48-2.29 (m, 1H), 2.05-0.64 (m, 47H); LC/MS: m/z calculated 737.4, found 738.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 16 
     Compound 49 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Compound 48 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate 
     To a solution of 19 (500 mg, 0.559 mmol) in dichloromethane (30 mL) stirred at rt was added Ac 2 O (0.158 mL, 1.677 mmol), triethylamine (0.195 mL, 1.397 mmol) and DMAP (6.83 mg, 0.056 mmol). The reaction mixture was stirred at rt until TLC indicated SM disappeared. The mixture was worked up and purified by silica gel chromatography (PE:EA=5:1) to give 48 (510 mg, 0.517 mmol, 93% yield). The product consisted of a mixture of rotomers.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.39-7.20 (m, 2H), 7.21-6.99 (m, 2H), 5.92-5.56 (m, 1H), 5.00-3.88 (m, 2H), 3.40-1.96 (m, 8H), 1.97-0.58 (m, 67H). 
     Step B: Compound 49 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 48 (500 mg, 0.534 mmol) in DCM (30 mL) stirred at rt was added TFA (15 mL, 195 mmol). The reaction mixture was stirred at rt until LCMS and TLC indicated SM had disappeared. The solvent was evaporated and CH 3 CN and H 2 O were added and mixture was lyophilized to give 49 as a trifluoroacetic acid salt (400 mg, 0.439 mmol, 82% yield).  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.46-7.19 (m, 4H), 5.94-5.70 (m, 1H), 4.58-4.36 (m, 1H), 4.25-3.88 (m, 2H), 3.25-3.02 (m, 1H), 3.01-2.41 (m, 5H), 2.08 (s, 3H), 2.00-0.64 (m, 47H); LC/MS: m/z calculated 779.5, found 780.4 (M+1) + . 
     Example 17 
     Compound 50 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 16 and isolated as a TFA salt.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.49-7.30 (m, 4H), 5.85-5.71 (m, 1H), 4.59-4.40 (m, 1H), 4.31-4.03 (m, 2H), 3.41-2.79 (m, 4H), 2.79-2.50 (m, 2H), 2.37 (d, J=18.1 Hz, 2H), 2.02-0.63 (m, 49H); LC/MS: m/z calculated 779.5, found 780.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 18 
     Compound 51 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 2-(dimethylamino)acetaldehyde, hydrochloride (6.75 g, 54.6 mmol) in methanol (20 mL) was added 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, Trifluoroacetic acid salt (46) (9.5 g, 10.92 mmol). The pH was adjusted to 7-8 with Et 3 N. The reaction mixture was stirred at rt for 2 h. Sodium cyanoborohydride (0.686 g, 10.92 mmol) was then added and the mixture was stirred at rt overnight. After the reaction was complete, water (15 mL) and EtOAc (15 mL) were added, and then the organic phase was removed and concentrated under reduced pressure. The product was extracted with EtOAc (80 mL×3), the combined organic phase was washed with brine, dried, and concentrated. The product was purified by flash chromatography (DCM:EtOAc=2:1 to 1:1, then DCM:MeOH=100:1 to 20:1) to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (51) (6 g, 7.41 mmol, 67.9% yield) as white solid. Multiple batches of this material (were combined 95 g), dissolved in 600 mL of dichloromethane and washed with NaHCO 3  (400 mL*3) and the organic phase was dried over Na 2 SO 4 , filtered and concentrated. The solids were washed with a mixture of EtOAc: petroleum ether (600 mL), and filtered followed by lyophilization to provide the final title compound 62 g as a white solid.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.47-7.29 (m, 4H), 4.48 (dd, J=5.8, 10.3 Hz, 1H), 4.15-4.04 (m, 1H), 3.80 (d, J=13.8 Hz, 1H), 3.57 (d, J=14.1 Hz, 1H), 3.21-2.82 (m, 5H), 2.72-2.41 (m, 9H), 2.37-2.05 (m, 4H), 2.05-0.74 (m, 45H); LC/MS: m/z calculated 808.5, found 809.5 (M+1) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 19 
     Compound 56 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Compound 52 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-2-one 
     To a solution of 2-(dimethylamino)acetaldehyde (4.79 g, 38.8 mmol) in methanol (50 ml) and 1,2-dichloroethane (DCE) (25 ml) was added (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(4-chlorobenzylamino)acetyl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a,4,5,5a,6,7,7a,8,9,10,11,11a,11b,12,13,13a-hexadecahydro-3H-cyclopenta[a]chrysen-2(5bH)-one (15) (5 g, 7.75 mmol) at 0° C. The pH was adjusted to 7 with Et 3 N. The reaction mixture was stirred at rt for 1 h. Sodium cyanoborohydride (0.487 g, 7.75 mmol) was added and the mixture was stirred overnight. The reaction was diluted with water (40 ml), and extracted with DCM (60 ml×3). The combined organic layer was washed with brine (20 ml), dried over sodium sulfate and filtered to afford crude product (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a,4,5,5a,6,7,7a,8,9,10,11,11a,11b,12,13,13a-hexadecahydro-3H-cyclopenta[a]chrysen-2(5bH)-one (52) (5 g, 5.89 mmol, 76% yield) as a light yellow solid, which was used in the next step. LC/MS: m/z calculated 678.5, found 679.3 (M+1) + . 
     Step B: Compound 53 
     1-Tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     To a solution of 4-(tert-butoxy)-3,3-dimethyl-4-oxobutanoic acid (11.91 g, 58.9 mmol), N,N-dimethylpyridin-4-amine (4.50 g, 36.8 mmol) in DCM (20 mL) was added EDC (11.29 g, 58.9 mmol). The reaction mixture was stirred at rt for 1 h. Then, (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-2-one (52) (5 g, 7.36 mmol) was add. Upon completion, the mixture was washed with 2 M HCl, water and brine. The organic layer was dried over Na 2 SO 4 , filtered and concentrated to give the crude product. The product was purified by a silica gel column using dichloromethane/methanol (20:1) to afford 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (53) (1.8 g, 1.917 mmol, 26.1 yield) as an oil.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.27 (s, 4H), 4.60-4.39 (m, 1H), 3.82 (d, J=13.8 Hz, 1H), 3.59 (d, J=13.8 Hz, 1H), 3.50-3.05 (m, 3H), 2.82-2.63 (m, 2H), 2.63-0.53 (m, 67H). 
     Step C: Compounds 54 and 55 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (54) and 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (55) 
     To a solution of 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (53) (1.5 g, 1.737 mmol) in methanol (10 mL) was added NaBH 4  (0.131 g, 3.47 mmol). The reaction mixture was stirred at rt for 1 h. The mixture then was extracted with DCM, washed with water and brine. The organic layer was dried over Na 2 SO 4 , and concentrated to give the crude product. This was purified by preparative-HPLC then purified by SFC to get 54 (230 mg, 15%) and 55 (360 mg 23%). For 54:  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.45-7.18 (m, 4H), 4.49 (dd, J=5.6, 10.7 Hz, 1H), 4.04 (d, J=9.8 Hz, 1H), 3.92-3.47 (m, 3H), 3.46-0.45 (m, 72H); LC/MS: m/z calculated 864.6, found 865.4 (M+1) + . For 55:  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.37-7.19 (m, 4H), 4.50 (dd, J=5.5, 10.5 Hz, 1H), 4.18-3.98 (m, 1H), 3.75 (d, J=13.3 Hz, 1H), 3.55 (d, J=13.3 Hz, 1H), 3.29-3.10 (m, 1H), 3.10-2.97 (m, 1H), 2.81-0.63 (m, 70H); LC/MS: m/z calculated 864.6, found 865.9 (M+1) + . 
     Step D: Compound 56 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 55 (360 mg, 0.416 mmol) in DCM (5 mL) stirred at rt was added TFA (2 mL, 26.0 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was evaporated to afford the crude product. This material was purified by preparative-HPLC to provide the title compound 56 as a TFA salt (300 mg, 0.285 mmol, 68.6% yield) as a white solid.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.58-7.34 (m, 4H), 4.50 (dd, J=5.0, 11.0 Hz, 1H), 4.23-4.10 (m, 1H), 4.09-3.74 (m, 2H), 3.58-3.12 (m, 3H), 3.12-2.37 (m, 10H), 2.12-0.67 (m, 50H); LC/MS: m/z calculated 808.5, found 809.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 20 
     Compound 57 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 2-(dimethylamino)acetaldehyde, hydrochloride (238 mg, 1.922 mmol) in methanol (12 mL) was added 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (50) (150 mg, 0.192 mmol). The reaction mixture was stirred at rt for 2 h then sodium cyanoborohydride (121 mg, 1.922 mmol) was added and the mixture was stirred overnight. Upon completion, the mixture was purified preparative-HPLC to provide the title compound (50 mg, 24%).  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.35-7.12 (m, 4H), 5.65 (d, J=9.3 Hz, 1H), 4.49 (dd, J=5.8, 10.0 Hz, 1H), 3.83 (d, J=13.6 Hz, 1H), 3.55 (d, J=13.6 Hz, 1H), 3.25-2.91 (m, 7H), 2.91-2.73 (m, 7H), 2.73-2.52 (m, 3H), 2.33 (d, J=18.1 Hz, 1H), 1.99-0.72 (m, 48H); LC/MS: m/z calculated 850.5, found 851.4 (M+1) + . 
     Example 21 
     Compound 58 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Acetoxy-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 20.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.40-7.10 (m, 4H), 5.68 (d, J=9.8 Hz, 1H), 4.55-4.39 (m, 1H), 3.81 (d, J=13.8 Hz, 1H), 3.57 (d, J=13.8 Hz, 1H), 3.29-2.74 (m, 11H), 2.74-2.27 (m, 6H), 2.14 (s, 3H), 2.09-0.68 (m, 46H); LC/MS: m/z calculated 850.5, found 851.4 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 22 and 23 
     Compound 62 and 63 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid (62) and 5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid (63) 
     
       
         
         
             
             
         
       
     
     Step A: Compound 59 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((Tert-butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     To a solution of tert-butyl 4-chlorobenzyl(2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-oxoethyl)carbamate (15) (1 g, 1.412 mmol), DMAP (0.517 g, 4.23 mmol) in pyridine (2 ml) stirred at rt was added 4,4-dimethyl-dihydro-3H-pyran-2,6-dione (1.003 g, 7.06 mmol). The reaction mixture was stirred at 50° C. for 5 h. The mixture was diluted with EtOAc and washed with water. The resultant extract was purified by silica gel column to provide 59 (800 mg, 60%).  1 H NMR (500 MHz, CHLOROFORM-d) δ=8.67 (br. s., 1H), 7.98-7.36 (m, 1H), 7.33-7.27 (m, 2H), 7.21-7.09 (m, 2H), 4.62-4.26 (m, 3H), 4.07-3.42 (m, 2H), 3.30-3.05 (m, 1H), 2.66-0.66 (m, 61H). 
     Step B: Compound 60 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     To a solution of 5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid (59) (800 mg, 0.941 mmol) in dichloromethane (6 mL) stirred at rt was added trifluoroacetic acid (2 mL, 0.941 mmol). The reaction mixture was stirred at rt for 1 h. The mixture was evaporated to provide the target compound (600 mg, 81%) which was used without further purification. LC/MS: m/z calculated 749.4, found 750.1 (M+1) + . 
     Step C: Compound 61 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     To a solution of 5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid, trifluoroacetic acid salt (60) (700 mg, 0.810 mmol) in methanol (30 ml) was added 2-(dimethylamino)acetaldehyde (353 mg, 4.05 mmol) at 0° C. The pH was adjusted to 7 with Et 3 N. The reaction mixture was stirred at rt for 1 h and sodium cyanoborohydride (50.9 mg, 0.810 mmol) was added and the resultant mixture was stirred overnight. The reaction was diluted with water (40 ml) and extracted with DCM (60 ml×3). The combined organic layer was washed with brine (20 ml), dried over sodium sulfate and filtered to afford crude product 5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid, trifluoroacetic acid salt (61) (200 mg, 23% yield) as a light yellow solid.  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.39 (s, 4H), 4.49 (dd, J=5.7, 10.7 Hz, 1H), 3.95 (d, J=13.6 Hz, 1H), 3.57 (d, J=13.2 Hz, 1H), 3.40-3.29 (m, 1H), 3.27-3.16 (m, 2H), 3.05 (s, 6H), 3.00-2.88 (m, 2H), 2.57-2.26 (m, 6H), 2.09 (d, J=19.5 Hz, 1H), 2.04-0.75 (m, 47H); LC/MS: m/z calculated 820.5, found 821.3 (M+1) + . 
     Step D: Compounds 62 and 63 
     5-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((4-Chlorobenzyl)(isopentyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid (62) and 5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(isopentyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid (63) 
     To a solution of 5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid, trifluoroacetic acid salt (61) (140 mg, 0.133 mmol) in methanol (10 mL) stirred at 0° C. was added NaBH 4  (10.09 mg, 0.267 mmol). The reaction mixture was stirred at 0° C. for 1 h, then more NaBH 4  (10.09 mg, 0.267 mmol) was added. After an additional 1 h at 0° C., the mixture was warmed to rt stirred for another 1 h. LCMS indicated the reaction was complete. EtOAc was added and the mixture was washed with water. The organic phase was evaporated to give the crude which was purified by preparative-HPL to give two diastereomers. 5 mg of isomer A (62) was isolated (3.5% yield) and 10 mg of isomer B (63) was isolated (7%). The stereochemistry of the newly formed stereocenter in each isomer was assigned by spectral similarity to compounds 51 (analogous to 62) and 56 (analogous to 63). For isomer A (62);  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.48-7.30 (m, 4H), 4.57-4.43 (m, 1H), 4.14 (d, J=10.1 Hz, 1H), 3.91-3.74 (m, 1H), 3.67 (d, J=13.9 Hz, 1H), 3.26-3.10 (m, 2H), 3.10-2.93 (m, 2H), 2.93-2.81 (m, 6H), 2.68-2.10 (m, 9H), 2.06-0.65 (m, 46H); LC/MS: m/z calculated 822.5, found 823.5 (M+1) + . For isomer B (63); 1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.43 (br. s., 4H), 4.51 (dd, J=5.8, 9.9 Hz, 1H), 4.16 (d, J=10.1 Hz, 1H), 3.98-3.75 (m, 2H), 3.52-3.37 (m, 1H), 3.31-3.02 (m, 3H), 3.02-2.71 (m, 9H), 2.64-2.34 (m, 6H), 2.19-0.69 (m, 46H); LC/MS: m/z calculated 822.5, found 823.4 (M+1) + . 
     Example 24 
     Compound 64 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 14. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.73-7.38 (m, 4H), 4.65-4.37 (m, 4H), 3.44 (d, J=12.3 Hz, 1H), 3.39-3.22 (m, 1H), 3.11-2.96 (m, 2H), 2.62 (q, J=16.0 Hz, 2H), 2.48 (d, J=18.3 Hz, 1H), 2.21-0.77 (m, 46H); LC/MS: m/z calculated 737.4, found 738.3 (M+1) + . 
     Example 25 
     Compound 65 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 14. Stereochemistry was tentatively assigned as drawn but not fully confirmed  6 spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.63-7.37 (m, 4H), 4.55-4.31 (m, 4H), 3.26-3.11 (m, 1H), 3.01-2.46 (m, 6H), 2.36-2.16 (m, 1H), 2.15-0.79 (m, 45H); LC/MS: m/z calculated 737.4, found 738.0 (M+1) + . 
     Example 26 
     Compound 66 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-((4-fluorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to example 16. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=10.54-8.79 (m, 1H), 7.50-7.34 (m, 2H), 7.19-6.99 (m, 2H), 5.81 (d, J=9.8 Hz, 1H), 4.49 (dd, J=5.8, 10.3 Hz, 1H), 4.31-3.93 (m, 2H), 3.26 (d, J=12.5 Hz, 1H), 3.20-3.05 (m, 1H), 3.05-2.82 (m, 2H), 2.80-2.47 (m, 2H), 2.36 (d, J=18.1 Hz, 1H), 2.05-0.57 (m, 49H); LC/MS: m/z calculated 763.5, found 764.3 (M+1) + . 
     Example 27 
     Compound 67 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((4-fluorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to example 16. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=9.65 (br. s., 1H), 7.46-7.28 (m, 2H), 7.03 (t, J=8.2 Hz, 2H), 5.86 (br. s., 1H), 4.50 (dd, J=5.5, 10.3 Hz, 1H), 4.29-4.06 (m, 1H), 4.06-3.85 (m, 1H), 3.19-2.99 (m, 1H), 2.81-2.39 (m, 6H), 2.21-0.62 (m, 49H); LC/MS: m/z calculated 763.5, found 764.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 28 
     Compound 68 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((4-fluorobenzyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (50) (150 mg, 0.192 mmol) in MeOH (6 mL) stirred in air at rt was added formaldehyde (31.2 mg, 0.384 mmol). The reaction mixture was stirred at rt for 2 h. Then it was added NaCNBH 3  (85 mg, 1.345 mmol) portionwise and stirred for overnight. The solvent was evaporated and the the residue diluted with DCM and washed with water and brine. The dried organics were filtered and concentrated to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((4-chlorobenzyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (68) (120 mg, 65% yield) as a white solid. This material was used without further purification.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.31-7.26 (m, 2H), 7.24-7.16 (m, 2H), 5.65 (d, J=8.5 Hz, 1H), 4.50 (br. s., 1H), 3.66-3.51 (m, 1H), 3.49-3.36 (m, 1H), 3.27-3.06 (m, 2H), 2.73-2.32 (m, 5H), 2.26 (s, 3H), 2.08-0.73 (m, 49H); LC/MS: m/z calculated 793.5, found 794.3 (M+1) + . 
     Example 29 
     Compound 69 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)(methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((4-chlorobenzyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (68) (120 mg, 0.151 mmol) in ethanol (2 mL) and toluene (2 mL) was added potassium hydroxide (15.25 mg, 0.272 mmol). The reaction mixture was stirred at rt for 30 min. The reaction mixture was neutralized with aqueous 1N HCl to pH=7 and evaporated to obtain the residue. This was extracted with DCM, washed with water and dried to yield the crude product which was purified by preparative-HPLC to afford 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid as a trifluoroacetate salt (95 mg, 72% yield) as a white solid. 1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.55 (s, 4H), 4.60-4.12 (m, 3H), 3.32-3.19 (m, 1H), 3.18-2.84 (m, 5H), 2.71-2.52 (m, 2H), 2.11-0.78 (m, 49H); LC/MS: m/z calculated 751.5, found 752.4 (M+1) + . 
     Example 30 
     Compound 70 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Acetoxy-2-((4-chlorobenzyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in similar manner to example 28.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.31-7.23 (m, 2H), 7.22-7.13 (m, 2H), 5.66 (dd, J=2.4, 8.7 Hz, 1H), 4.55-4.43 (m, 1H), 3.51-3.35 (m, 2H), 3.19-3.05 (m, 1H), 2.74-2.42 (m, 5H), 2.42-0.66 (m, 53H); LC/MS: m/z calculated 793.5, found 794.5 (M+1) + . 
     Example 31 
     Compound 71 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((4-Chlorobenzyl)(methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in similar manner to example 29.  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.63-7.38 (m, 4H), 4.56-4.25 (m, 4H), 3.25-3.14 (m, 1H), 2.99 (s, 3H), 2.82-2.52 (m, 4H), 2.52-2.32 (m, 2H), 2.24 (d, J=12.9 Hz, 1H), 2.09-0.77 (m, 45H); LC/MS: m/z calculated 751.5, found 752.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 32 
     Compound 72 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-(N-(4-chlorobenzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     A mixture of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (50) (200 mg, 0.256 mmol), TEA (0.332 mL, 2.56 mmol), DMAP (6.3 mg, 0.05 mmol) and in DCM (5 ml) was stirred for 3 h. The mixture was quenched with water (50 ml), washed with water (2×50 ml), dried over Na 2 SO 4 , filtered and concentrated in vacuo to afford 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-(N-(4-chlorobenzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (72) (160 mg, 0.182 mmol, 71.0% yield) as a white solid.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.42-7.24 (m, 2H), 7.24-6.90 (m, 2H), 5.98-5.68 (m, 1H), 5.15-4.56 (m, 1H), 4.56-4.45 (m, 1H), 4.40-3.98 (m, 2H), 2.29-0.68 (m, 66H), 3.48-0.58 (m, 58H); LC/MS: m/z calculated 821.5, found 822.3 (M+1) + . 
     Example 33 
     Compound 73 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(N-(4-Chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-(N-(4-chlorobenzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (72) (150 mg, 0.182 mmol) in ethanol (2 mL) and toluene (2 mL) was added potassium hydroxide (40.9 mg, 0.729 mmol). The reaction mixture was stirred at rt for 30 min. The reaction mixture was neutralized with aqueous 1 N HCl to pH=7 and concentrated to obtain a residue. The residue was extracted with DCM, washed with water and dried to yield the crude product which was purified by preparative-HPLC to afford 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(N-(4-chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (73) (40 mg, 28%) as a white solid. Compound exists as a mixture of rotomers;  1 H NMR (400 MHz, DMSO-d 6 ) δ=12.18 (br. s., 1H), 7.52-7.14 (m, 4H), 5.03-3.77 (m, 5H), 3.39-2.80 (m, 3H), 2.62-2.54 (m, 1H), 2.27 (t, J=17.2 Hz, 1H), 2.20-0.58 (m, 51H); LC/MS: m/z calculated 779.5, found 802.3 (M+Na) + . 
     
       
         
         
             
             
         
       
     
     Example 34 
     Compound 74 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-Chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     A mixture of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (49) (150 mg, 0.192 mmol), Na 2 CO 3  (20.37 mg, 0.192 mmol), in methanol (3 mL) was stirred for 3 h, then purified by preparative-HPLC to afford 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (74) (45 mg, 30%) as a white solid. Compound exists as a mixture of rotomers;  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.43-7.27 (m, 2H), 7.28-7.11 (m, 2H), 5.30-4.09 (m, 6H), 3.55-2.09 (m, 11H), 2.09-0.78 (m, 44H); LC/MS: m/z calculated 779.5, found 780.4 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 35 
     Compound 76 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(N-(2-Chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     To a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((tert-butoxycarbonyl)(2-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (75, made in a similar manner to compound 48) (100 mg, 0.107 mmol) in DCM (30 mL) stirred at rt was added TFA (15 mL, 195 mmol). The reaction mixture was stirred at rt until LCMS and TLC indicated SM disappeared. The solvent was removed to give the crude product purified by preparative HPLC to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(N-(2-chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (40 mg, 48%). Stereochemistry was tentatively assigned as drawn, but not fully confirmed spectroscopically. The compound exists as a mixture of rotomers.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.55-7.14 (m, 4H), 5.10-4.72 (m, 2H), 4.72-3.97 (m, 3H), 3.54-3.39 (m, 1H), 3.30-3.21 (m, 1H), 3.13-2.97 (m, 1H), 2.84-0.69 (m, 53H); LC/MS: m/z calculated 779.5, found 780.3 (M+1) + . 
     Example 36 
     Compound 77 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-((2-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 20. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.64-7.06 (m, 4H), 5.61 (d, J=8.8 Hz, 1H), 4.50 (dd, J=5.4, 10.7 Hz, 1H), 3.82 (d, J=13.8 Hz, 1H), 3.65 (d, J=13.6 Hz, 1H), 3.26-2.92 (m, 7H), 2.89-2.74 (m, 7H), 2.74-2.53 (m, 3H), 2.26 (d, J=18.3 Hz, 1H), 1.93-0.67 (m, 48H); LC/MS: m/z calculated 850.5, found 851.4 (M+1) + . 
     Example 37 
     Compound 78 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((3-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 20 as a TFA salt. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.61 (s, 1H), 7.56-7.43 (m, 3H), 4.57-4.44 (m, 2H), 4.35-4.21 (m, 2H), 3.38 (dd, J=2.5, 12.5 Hz, 1H), 3.31-3.23 (m, 1H), 3.09-3.01 (m, 1H), 2.96 (t, J=11.8 Hz, 1H), 2.71-2.51 (m, 1H), 2.46 (d, J=18.3 Hz, 1H), 2.15-0.79 (m, 48H); LC/MS: m/z calculated 737.4, found 738.3 (M+1) + . 
     Example 38 
     Compound 79 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 20 as a TFA salt. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.77-7.28 (m, 4H), 4.68-4.15 (m, 4H), 3.27-3.11 (m, 1H), 2.73-2.48 (m, 5H), 2.38-2.17 (m, 1H), 2.16-0.71 (m, 4H); LC/MS: m/z calculated 737.4, found 738.4 (M+1) + . 
     Example 39 
     Compound 80 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-((3-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 16 as a TFA salt. Stereochemistry was tentatively assigned as drawn, but not fully confirmed spectroscopically.  1 H NMR (500 MHz, CHLOROFORM-d) δ=7.43 (s, 1H), 7.42-7.32 (m, 3H), 5.81 (d, J=10.1 Hz, 1H), 4.49 (dd, J=5.5, 10.2 Hz, 1H), 4.24 (d, J=13.2 Hz, 1H), 4.13-3.96 (m, 1H), 3.34-3.04 (m, 2H), 3.05-2.82 (m, 2H), 2.75-2.62 (m, 1H), 2.62-2.52 (m, 1H), 2.37 (d, J=18.0 Hz, 1H), 1.93 (s, 3H), 1.90-0.65 (m, 47H); LC/MS: m/z calculated 779.5, found 780.3 (M+1) + . 
     Example 40 
     Compound 81 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Acetoxy-2-((3-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 16 as a TFA salt. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (500 MHz, CHLOROFORM-d) δ=9.77 (br. s., 1H), 7.50-7.09 (m, 4H), 5.87 (br. s., 1H), 4.57-4.44 (m, 1H), 4.12 (d, J=1.6 Hz, 1H), 3.96 (d, J=13.2 Hz, 1H), 3.23-3.00 (m, 1H), 2.86-2.37 (m, 5H), 2.20-0.66 (m, 50H); LC/MS: m/z calculated 779.5, found 780.2 (M+1) + . 
     Example 41 
     Compound 82 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((3-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 18 as a TFA salt. To a solution of 2-(dimethylamino)acetaldehyde (553 mg, 4.47 mmol) in methanol (10 ml), was added 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((3-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, trifluoroacetic acid salt (80) (400 mg, 0.447 mmol). The reaction mixture was stirred at 40° C. for 2 h. The reaction was cooled to rt and NaBH 3 CN (281 mg, 4.47 mmol) was added. The mixture and stirred overnight. Then concentrated in vacuo and purified by preparative-HPLC to afford 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((3-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (82) (80 mg, 20%) as a light yellow solid. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 850.5, found 851.5 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 42 and 43 
     Compound 83 and 84 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((3-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (83) and 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-chlorobenzyl)(ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) oxy)-2,2-dimethyl-4-oxobutanoic acid (84) 
     
       
         
         
             
             
         
       
     
     To a solution of 2-(dimethylamino)acetaldehyde, hydrochloride (553 mg, 4.47 mmol) in methanol (10 ml) was added (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(4-chlorobenzylamino)acetyl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a,4,5,5a,6,7,7a,8,9,10,11,11a,11b,12,13,13a-hexadecahydro-3H-cyclopenta[a]chrysen-2(5bH)-one (81) (15 g, 24.66 mmol). The reaction mixture was stirred at 40° C. for 2 h. The reaction was cooled to rt and sodium cyanoborohydride (7.75 g, 123 mmol) was added and the resultant mixture was stirred overnight. The reaction was diluted with ammonium chloride solution (40 ml), and extracted with DCM (60 ml×3). The combined organic layer was washed with brine (20 ml), dried over sodium sulfate, filtered and concentrated in vacuo to afford crude product which was purified by preparative-HPLC to give 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((3-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (83) (80 mg, 20%) and 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-chlorobenzyl)(ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (84) (14 mg, 3%) as their respective TFA salts. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. For 83;  1 H NMR (500 MHz, CHLOROFORM-d) δ=7.25-7.10 (m, 3H), 7.04 (d, J=3.8 Hz, 1H), 5.57 (d, J=9.1 Hz, 1H), 4.42 (dd, J=5.0, 10.7 Hz, 1H), 3.72 (d, J=13.9 Hz, 1H), 3.42 (d, J=14.2 Hz, 1H), 3.16-2.90 (m, 6H), 2.90-2.79 (m, 1H), 2.79-2.65 (m, 7H), 2.65-2.41 (m, 3H), 2.22 (d, J=18.3 Hz, 1H), 1.89 (s, 3H), 1.84-0.62 (m, 45H); LC/MS: m/z calculated 850.5, found 851.4 (M+1) + . For 84;  1 H NMR (400 MHz, METHANOL-d 4 ) □=7.65 (s, 1H), 7.62-7.46 (m, 3H), 4.58-4.28 (m, 4H), 3.49-3.38 (m, 1H), 3.31-3.23 (m, 2H), 3.13-2.91 (m, 2H), 2.62 (q, J=15.9 Hz, 2H), 2.10-0.76 (m, 51H); LC/MS: m/z calculated 765.5, found 766.5 (M+1) + . 
     Example 44 
     Compound 85 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((3-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 33. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.57-7.31 (m, 4H), 4.50 (dd, J=5.0, 11.0 Hz, 1H), 4.28 (br. s., 1H), 4.02-3.69 (m, 2H), 3.53-3.36 (m, 1H), 3.32-3.07 (m, 4H), 3.08-2.85 (m, 2H), 2.78 (s, 6H), 2.73-2.51 (m, 3H), 2.45 (d, J=18.3 Hz, 1H), 2.13-0.75 (m, 46H); LC/MS: m/z calculated 808.5, found 809.5 (M+1) + . 
     Example 45 
     Compound 86 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-Chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 33. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.46-7.23 (m, 4H), 4.49 (dd, J=5.1, 10.9 Hz, 1H), 4.19 (d, J=10.0 Hz, 1H), 3.95-3.78 (m, 1H), 3.78-3.66 (m, 1H), 3.41-3.33 (m, 1H), 3.27-3.00 (m, 3H), 2.87 (s, 6H), 2.70-2.50 (m, 3H), 2.37 (br. s., 1H), 2.34-2.16 (m, 3H), 2.08-0.82 (m, 46H); LC/MS: m/z calculated 808.5, found 809.5 (M+1) + . 
     Example 46 
     Compound 87 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(3-chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 33 from compound 81. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. The compound exists as a mixture of rotomers.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.42-7.04 (m, 4H), 5.42-3.97 (m, 3H), 3.55-3.00 (m, 3H), 2.82-0.79 (m, 53H); LC/MS: m/z calculated 779.5, found 780.3 (M+1) + . 
     Example 47 
     Compound 88 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(N-(3-chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to example 33 from compound 80. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.36-6.99 (m, 4H), 4.89-3.88 (m, 4H), 3.39-2.83 (m, 3H), 2.73-0.66 (m, 53H); LC/MS: m/z calculated 779.5, found 780.3 (M+1) + . 
     Example 48 and 49 
     Compounds 89 and 90 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((2-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (89) and 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-chlorobenzyl)(ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (90) 
     
       
         
         
             
             
         
       
     
     The title compounds were made in a similar manner to examples 42 and 43. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. For 89;  1 H NMR (400 MHz, CHLOROFORM-d) δ=7.42-7.18 (m, 4H), 5.70-5.56 (m, 1H), 4.57-4.42 (m, 1H), 3.83-3.51 (m, 2H), 3.21-2.85 (m, 3H), 2.79 (s, 6H), 2.74-2.54 (m, 3H), 2.54-2.23 (m, 4H), 2.15-0.65 (m, 50H); LC/MS: m/z calculated 850.5, found 851.4 (M+1) + . For 90;  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.77-7.43 (m, 4H), 4.69-4.27 (m, 4H), 3.62-3.37 (m, 2H), 3.26-3.10 (m, 1H), 2.97-2.82 (m, 1H), 2.61 (q, J=16.0 Hz, 2H), 2.27-2.10 (m, 1H), 2.10-0.77 (m, 51H); LC/MS: m/z calculated 765.5, found 766.3 (M+1) + . 
     Example 50 
     Compound 91 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 33. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.55-7.42 (m, 2H), 7.42-7.32 (m, 2H), 4.49 (dd, J=5.5, 11.0 Hz, 1H), 4.43-4.30 (m, 1H), 4.00-3.89 (m, 1H), 3.87-3.76 (m, 1H), 3.42-3.34 (m, 1H), 3.24-2.91 (m, 3H), 2.91-2.51 (m, 10H), 2.52-2.17 (m, 4H), 2.11-1.84 (m, 2H), 1.84-0.78 (m, 44H); LC/MS: m/z calculated 808.5, found 809.4 (M+1) + . 
     Example 51 
     Compound 92 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made in a similar manner to Example 33. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, METHANOL-d 4 ) δ=7.57-7.45 (m, 2H), 7.39 (dd, J=3.6, 5.6 Hz, 2H), 4.56-4.40 (m, 2H), 4.02 (d, J=12.8 Hz, 1H), 3.81 (d, J=12.8 Hz, 1H), 3.46-3.23 (m, 2H), 3.13-2.89 (m, 4H), 2.81-2.41 (m, 10H), 2.15-1.91 (m, 3H), 1.89-0.82 (m, 44H); LC/MS: m/z calculated 808.5, found 809.5 (M+1) + . 
     Example 52 
     Compound 93 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Acetoxy-2-(benzylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 16. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (500 MHz, DMSO-d 6 ) δ=12.17 (br. s., 1H), 9.49 (br. s., 1H), 9.19 (br. s., 1H), 7.66-7.28 (m, 5H), 5.86-5.53 (m, 1H), 4.49-3.97 (m, 3H), 3.16-2.84 (m, 2H), 2.68-2.55 (m, 1H), 2.55-2.47 (m, 2H), 2.41 (d, J=19.5 Hz, 1H), 2.11 (s, 3H), 2.04-0.67 (m, 47H); LC/MS: m/z calculated 745.5, found 746.5 (M+1) + . 
     Example 53 
     Compound 94 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-Acetoxy-2-(benzylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 16. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (500 MHz, CHLOROFORM-d) δ=7.41 (br. s., 5H), 5.81 (d, J=10.1 Hz, 1H), 4.48 (dd, J=5.5, 10.2 Hz, 1H), 4.25 (d, J=13.2 Hz, 1H), 4.06 (d, J=13.2 Hz, 1H), 3.27 (d, J=12.3 Hz, 1H), 3.14 (dt, J=6.8, 13.6 Hz, 1H), 3.08-2.85 (m, 2H), 2.74-2.62 (m, 1H), 2.62-2.54 (m, 1H), 2.36 (d, J=18.0 Hz, 1H), 1.99-0.63 (m, 49H); LC/MS: m/z calculated 745.5, found 746.5 (M+1) + . 
     Example 54 
     Compound 95 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-Chloro-2-fluorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 14. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 755.4, found 756.3 (M+1) + . 
     Example 55 
     Compound 96 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((3-chloro-2-fluorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 14. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 755.4, found 756.2 (M+1) + . 
     Example 56 
     Compound 97 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-Chloro-2-fluorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 18. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (500 MHz, CHLOROFORM-d) δ=7.49-7.35 (m, 2H), 7.15 (t, J=7.7 Hz, 1H), 4.46 (dd, J=6.1, 9.9 Hz, 1H), 4.22 (d, J=10.4 Hz, 1H), 4.05 (d, J=13.9 Hz, 1H), 3.90 (d, J=13.6 Hz, 1H), 3.48 (br. s., 1H), 3.32-3.02 (m, 4H), 2.85 (br. s., 6H), 2.74-2.40 (m, 4H), 2.40-2.15 (m, 3H), 1.99-0.58 (m, 43H); LC/MS: m/z calculated 826.5, found 827.3 (M+1) + . 
     Example 57 
     Compound 98 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((3-Chloro-2-fluorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 18. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (500 MHz, CHLOROFORM-d) δ=7.56-7.32 (m, 2H), 7.16 (t, J=7.7 Hz, 1H), 4.53-4.38 (m, 1H), 4.28 (d, J=9.1 Hz, 1H), 4.13-3.87 (m, 2H), 3.44 (br. s., 1H), 3.33-2.91 (m, 5H), 2.93-2.21 (m, 11H), 2.09-1.95 (m, 1H), 1.95-0.65 (m, 45H); LC/MS: m/z calculated 826.5, found 827.3 (M+1) + . 
     Example 58 
     Compound 99 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-Hydroxy-2-(isopropylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 18. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=10.30 (br. s., 1H), 4.71-4.26 (m, 2H), 3.49-2.98 (m, 2H), 2.87-2.42 (m, 6H), 2.42-2.21 (m, 1H), 2.07-0.68 (m, 53H); LC/MS: m/z calculated 655.5, found 656.5 (M+1) + . 
     Example 59 
     Compound 100 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(cyclohexylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     The title compound was made as a TFA salt in a similar manner to Example 18. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=10.25 (br. s., 1H), 7.12 (br. s., 1H), 4.67-4.34 (m, 2H), 3.29-3.03 (m, 1H), 2.92 (br. s., 1H), 2.83-2.42 (m, 6H), 2.42-2.26 (m, 1H), 2.11-0.72 (m, 55H); LC/MS: m/z calculated 695.5, found 696.4 (M+1) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 60 and 61 
     Compound 111 and 112 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (111) and 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (112) 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 101 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((1,3-Dithian-2-yl)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-2-one 
     To a solution of 1,3-dithiane (5.7 g, 47.4 mmol) in anhydrous tetrahydrofuran (THF, 60 mL) under an atmosphere of nitrogen at −40° C. was slowly added a solution of n-BuLi (27 mL, 67.5 mmol). After the reaction mixture was stirred at −20° C. for another 2 h, a solution of the intermediate 6 (4.2 g, 8.46 mmol) in anhydrous THF (40 mL) was slowly added under an atmosphere of nitrogen at −70° C. The reaction was then stirred at −78° C. for 1 h before it was quenched with a saturated solution of NaHCO 3 . Extraction was conducted with EtOAc and the organic phase was washed with water (50 mL), saturated brine (50 mL), dried over sodium sulfate, and evaporated under reduced pressure to provide a crude product, which was purified by column chromatography on silica gel (PE:EtOAc=8:1 to 4:1) to afford the intermediate 101 (3.0 g, 5.22 mmol, 61.7%). LC/MS: m/z calculated 574.4, found 575.0 (M+1) + . 
     Step B: Intermediate 102 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(Acetoxy(1,3-dithian-2-yl)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 101 (3.5 g, 6.09 mmol), Et 3 N (2.55 mL, 18.26 mmol), and DMAP (0.149 g, 1.218 mmol) in DCM (40 mL) was added Ac 2 O (3.45 mL, 36.5 mmol) at room temperature. After stirring at 50° C. for 2 h, the reaction mixture was quenched with water. The organic phase was washed with water (100 mL), dried over sodium sulfate, and evaporated under reduced pressure to provide the intermediate 102 (3.41 g, 85%). LC/MS: m/z calculated 658.4, found 659.1 (M+1) + . 
     Step C: Intermediate 103 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((1,3-Dithian-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     A solution of 102 (6.7 g, 10.17 mmol) and potassium hydroxide (1.141 g, 20.33 mmol) in a mixture 1:1 of toluene and ethanol (100 ml) was stirred vigorously at rt for 1 hr. The reaction mixture was neutralized with aqueous 1N HCl to pH 7 and reduced to dryness. The residue was purified on a silica gel using Petroleum ether/EtOAc (5:1) to yield (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((1,3-dithian-2-yl)(hydroxy)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (103) (3.4 g, 5.01 mmol, 49%) as a light yellow compound. LC/MS: m/z calculated 616.4, found 617.3 (M+1) + . 
     Step D: Intermediate 104 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(1-hydroxy-2-oxoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of 103 (1.2 g, 1.945 mmol) in acetonitrile (20 ml) and water (5.00 ml) stirred in air at rt was added NBS (1.385 g, 7.78 mmol) in one charge. The reaction mixture was stirred at rt for 0.5 h, then quenched with Na 2 SO 3  (solid), concentrated and the residue was extracted with EtOAc. The organic phase was washed with water and saturated brine, dried over sodium sulphate and concentrated in vacuo to give (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(1-hydroxy-2-oxoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (104) (1.09 g, 1.083 mmol, 55.7% yield) as a light yellow solid which was used without further purification. 
     Step E: Intermediate 106 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((1-(5-Chloropyrimidin-2-yl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(1-hydroxy-2-oxoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (104) (545 mg, 1.035 mmol) and 1-(5-chloropyrimidin-2-yl)cyclopropanamine, hydrochloride (105) (213 mg, 1.035 mmol) in methanol (5 ml) and 1,2-dichloroethane (5 ml) was added zinc chloride (141 mg, 1.035 mmol). The reaction mixture was stirred at room temperature overnight. and then sodium cyanoborohydride (65.0 mg, 1.035 mmol) was added. The reaction mixture was allowed to stir for 1 h, then silica gel was added to the mixture and the solvents removed in vacuo to give the residue-silica gel powder which was purified on a silica gel column using petroleum ether/EtOAc (5:1 to 3:1) to afford (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((1-(5-chloropyrimidin-2-yl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (105) as a mixture of diastereomers (200 mg, 0.166 mmol, 16.04% yield) as a yellow foam. LC/MS: m/z calculated 679.4, found 680.3 (M+1) + . 
     Step F: Intermediate 107 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(3-(1-(5-Chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((1-(5-chloropyrimidin-2-yl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (106) (400 mg, 0.588 mmol) in dichloromethane (15 ml) stirred at rt was added BOC 2 O (1.365 ml, 5.88 mmol). The reaction mixture was stirred at rt overnight then evaporated under reduced pressure and the residue was subjected to a silica gel chromatography with petroleum ether/EtOAc (6:1 to 3:1) to give (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (107) (350 mg, 0.496 mmol, 84% yield) as a white solid. 
     Step G: Intermediate 108 
     3-(1-(5-Chloropyrimidin-2-yl)cyclopropyl)-5-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)oxazolidin-2-one 
     To a solution of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (107) (350 mg, 0.496 mmol) in 1,4-dioxane (10 mL) and methanol (5 mL) was added hydrochloric acid (5 mL, 165 mmol). The reaction mixture was stirred at 40° C. overnight. The solvents were removed under reduced pressure and taken up in DCM (100 ml), washed with sat. NaHCO 3  (20 ml), water (20 ml) and brine (20 ml), dried over sodium sulfate, filtered and concentrated to give residue, which was subjected to a silica gel column eluting with petroleum ether/EtOAc (6:1 to 3:1) to give 3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-5-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)oxazolidin-2-one (130 mg, 0.087 mmol, 17%) as a white solid. LC/MS: m/z calculated 663.4, found 664.3 (M+1) + . 
     Step H: Intermediates 109 and 110 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (109) and 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (110) 
     A mixture of 4-tert-butoxy-3,3-dimethyl-4-oxobutanoic acid 10 (119 mg, 0.587 mmol), 3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-5-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)oxazolidin-2-one (108) (130 mg, 0.196 mmol), EDC (188 mg, 0.978 mmol) and DMAP (71.7 mg, 0.587 mmol) in DCM (5 mL) was stirred at rt overnight. After the reaction finished, the mixture was diluted with DCM (25 ml), washed with water (2×15 ml) and brine (20 ml), dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue was purified by silica gel chromatography using petroleum ether/EtOAc (6:1 to 3:1) as eluent to give the two diastereomeric products 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (109) and 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (110) in the amounts of (80 mg, 45%) and (50 mg, 22%) as white solids. The stereochemical assignments for each diastereomer were not made. Compound A (tentatively designated as 109): LC/MS: m/z calculated 847.5, found 848.3 (M+1) + . Compound B (tentatively designated as 110): LC/MS: m/z calculated 847.5, found 848 (M+1) + . 
     Step I: Compound 111 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (109) (80 mg, 0.094 mmol) in DCM (10 mL) was added TFA (1 mL, 0.094 mmol). The reaction mixture was stirred at rt overnight, then diluted with DCM (20 ml) and washed with water (2×15 ml), saturated sodium bicarbonate solution (20 ml) and brine (20 ml), dried over sodium sulfate, filtered and concentrated to get a residue. The residue was purified on silica gel using petroleum ether/EtOAc (4:1 to 1:1) as eluent to give the product 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (111) (50 mg, 67%) as a white solid. This material was lyophilized to give a white powder. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=8.58 (s, 2H), 5.04 (t, J=8.5 Hz, 1H), 4.51 (dd, J=4.6, 10.9 Hz, 1H), 3.41-3.04 (m, 4H), 2.75-2.44 (m, 3H), 2.36 (d, J=13.3 Hz, 1H), 2.12-0.72 (m, 49H); LC/MS: m/z calculated 791.4, found 792.3 (M+1) + . 
     Step J: Compound 112 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-3-(1-(5-chloropyrimidin-2-yl)cyclopropyl)-2-oxooxazolidin-5-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     The title compound was made in a similar manner to that described for compound 111. Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically.  1 H NMR (400 MHz, CHLOROFORM-d) δ=8.59 (s, 2H), 5.10 (t, J=8.8 Hz, 1H), 4.52 (dd, J=5.3, 10.8 Hz, 1H), 3.70-3.54 (m, 2H), 3.28-3.10 (m, 2H), 3.08-2.92 (m, 2H), 2.77-2.53 (m, 2H), 2.14-0.58 (m, 49H); LC/MS: m/z calculated 791.4, found 792.3 (M+1) + . 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Example 62 
     Compound 124 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-Chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 113 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(Hydroxymethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 3 (5 g, 7.59 mmol) in EtOH (100 mL) and toluene (100 mL) was added KOH (0.51 g, 9.11 mmol). After stirring at room temperature for 4 h, the reaction mixture was then partitioned between water (500 mL) and EtOAc (500 mL). The organic phase was washed with water (200 mL×3), brine (100 mL), and dried over sodium sulfate. Removal of the solvent provided a residue, which was purified by column chromatography on silica gel (Hex:EtOAc=6:1 to 4:1) to afford the intermediate 113 (2.5 g, 67.9%) as a white solid.  1 H NMR (400 Hz, CDCl 3 ) δ ppm 4.50-4.67 (1H, m), 3.68 (1H, d, J=10.4 Hz), 3.32 (1H, d, J=10.4 Hz), 3.23-3.15 (1H, m), 2.42-2.28 (3H, m), 2.05 (3H, s), 2.02-1.89 (2H, m), 1.77-0.83 (40H, m). 
     Step B: Intermediate 114 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-Formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 113 (3 g, 6.19 mmol) in DCM (75 mL) at room temperature were added PCC (4 g, 18.57 mmol) and silica gel (3.0 g). After stirring at room temperature for 2 h, the reaction was quenched with water (100 mL). The organic phase was separated, washed with saturated sodium bicarbonate (50 mL), dried over sodium sulfate and concentrated in vacuo to provide a residue, which was purified by column chromatography on silica gel (Hex:EtOAc=10:1) to afford the intermediate 114 (3 g, 100%) as a white solid.  1 H NMR (400 Hz, CDCl 3 ) δ ppm 9.43 (1H, s), 4.50-4.46 (1H, m), 3.25-3.21 (1H, m), 2.43-2.02 (5H, m), 2.04 (3H, m), 2.00-1.93 (1H, m), 1.75-0.81 (38H, m). 
     Step C: Intermediate 115 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(1-Hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 114 (5 g, 10.36 mmol) in MeNO 2  (128 mL, 2382 mmol) was added Et 3 N (10.11 mL, 72.5 mmol). After it was stirred at 60° C. overnight, the reaction mixture was partitioned between water and EtOAc (100 mL each). The organic phase was washed with water (20 mL×3), brine (20 mL), and dried over sodium sulfate. Removal of the solvent provided a residue, which was purified by column chromatography on silica gel (Hex:EtOAc=10:1 to 6:1) to afford the intermediate 115 (2.8 g, 49.7%) as a white powder. LC/MS: m/z calculated 543.4, found 566.3 (M+Na + )+. 
     Step D: Intermediate 116 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-Amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 115 (2.8 g, 5.15 mmol) in MeOH (166 mL) were added nickel(II) chloride (1.67 g, 12.87 mmol) and sodium borohydride (4.87 g, 129 mmol) at 0° C. After stirring at 0° C. for 10 min, the reaction mixture was partitioned between water and EtOAc (200 mL each), and the organic phase was washed with water (100 mL×3), brine (50 mL), and dried over sodium sulfate. Removal of the solvent provided the intermediate 116 (2.65 g, 100%) as a solid. LC/MS: m/z calculated 513.4, found 514.3 (M+1)+. 
     Step E: Intermediate 118 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-Butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 116 (350 mg, 0.613 mmol) and 4-chlorobenzaldehyde (86 mg, 0.613 mmol) in MeOH (15 mL) and dichloroethane (DCE, 15 mL) was added zinc chloride (50.1 mg, 0.368 mmol). After the reaction mixture was stirred at 80° C. for 1 h and cooled down to room temperature, sodium cyanoborohydride (57.8 mg, 0.92 mmol) was added. The resulting mixture was stirred at room temperature for another 1 h to provide the intermediate 117. 
     To the reaction mixture obtained above were added Et 3 N (0.18 mL, 1.38 mmol) and di-tert-butyl dicarbonate (0.157 mL, 0.674 mmol). After stirring at room temperature for 30 min, the reaction mixture was partitioned between water (20 mL) and EtOAc (100 mL). The organic phase was washed with water (30 mL×3), brine (20 mL), and dried over sodium sulfate. Removal of the solvent provided a residue, which was purified by column chromatography on silica gel (Hex:EtOAc=15:1) to afford the intermediate 118 (125 mg, 27.6%) as a white solid. 
     Step F: Intermediate 119 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-Butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a solution of the intermediate 118 (120 mg, 0.162 mmol) in DCM (10 mL) were added PCC (35 mg, 0.162 mmol) and silica gel (100 mg). After stirring at room temperature for 2 h, the insoluble material was removed by filtration and the filtrate was concentrated to afford the intermediate 119 (110 mg, 92%) as a white solid. 
     Step G: Intermediate 120 
     tert-butyl 4-Chlorobenzyl(2-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-oxoethyl)carbamate 
     To a solution of NaOH (597 mg, 14.94 mmol) in MeOH (1 mL), THF (1 mL), and water (0.5 mL) was added the intermediate 119 (110 mg, 0.149 mmol). After stirring at room temperature for 1 h, the reaction was diluted with water (20 mL), and extracted with EtOAc (50 mL). The organic phase was washed with brine (20 mL), dried over sodium sulfate, and evaporated in vacuo to afford the intermediate 120 (100 mg, 96%) as a white solid. 
     Step H: Intermediate 121 
     4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate 
     To a solution of 4-tert-butoxy-3,3-dimethyl-4-oxobutanoic acid 10 (1.648 g, 8.15 mmol), DMAP (0.995 g, 8.15 mmol) in DCM (15 ml) stirred at rt was added EDCI (2.60 g, 13.58 mmol). The reaction mixture was stirred at rt. for 2 h. Then tert-butyl 4-chlorobenzyl(2-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-oxoethyl)carbamate 120 (1.886 g, 2.72 mmol) was added to the reaction. The reaction mixture was stirred at rt overnight. Upon completion, the mixture was washed with water (25 mL×2) and brine, dried over sodium sulfate filtered through a short silica gel column and evaporated in vacuo to give the crude product which was further purified on a silica gel column using petroleum ether/EtOAc (50:1 to 10:1) to give intermediate 121 (2.37 g, 99%) as white foam. 
     Step I: Intermediates 122 and 123 
     4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((tert-Butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (122) and 4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (123) 
     To a solution of and 4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)acetyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (121) (791 mg, 0.90 mmol) in MeOH (10 mL) and THF (10.00 mL) stirred at 0° C. was added NaBH 4  (170 mg, 4.50 mmol). The reaction mixture was stirred at rt for 2 h until starting material had disappeared. Upon completion, silica gel was added and the mixture evaporated to dryness and purified by silica gel chromatography (petroleum ether/EtOAc, 40:1 to 10:1) to give the two diastereomeric products: 4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate and 4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((tert-butoxycarbonyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate. Stereochemistry was not confirmed but the diastereomers were produced in the amount of (410 mg, 52%, tentatively assigned as compound 122) and (77 mg, 10%, tentatively assigned as compound 123) as white foams. 
     Step J: Compound 124 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 122 (410 mg, 0.466 mmol) in DCM (10 mL) was added TFA (2 mL, 26.0 mmol). The reaction mixture was stirred at rt for 2 h. The reaction mixture was concentrated to dryness and purified by preparative-HPLC to afford 4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, trifluoroacetic acid salt (124) (185 mg, 0.221 mmol, 47.4% yield) as white solid.  1 H NMR (500 MHz, CHLOROFORM-d) δ=7.36 (q, J=8.5 Hz, 4H), 4.58-4.41 (m, 1H), 4.41-4.31 (m, 1H), 4.20-3.97 (m, 2H), 3.23-3.05 (m, 2H), 2.85-2.66 (m, 2H), 2.56 (d, J=16.1 Hz, 1H), 2.51-2.38 (m, 1H), 2.34-2.19 (m, 2H), 1.93-1.82 (m, 1H), 1.83-0.67 (m, 46H); LC/MS: m/z calculated 723.5, found 724.3 (M+1) + . 
     
       
         
         
             
             
         
       
     
     Example 63 
     Compound 132 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(3,4-dihydroisoquinolin-2(1H)-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Step A: Intermediate 125 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-vinyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a cold (−78° C.) solution of methyltriphenylphosphonium bromide (8.63 g, 24.16 mmol) in THF (30 mL) was added lithium bis(trimethylsilyl)amide (4.4 g, 24.1 mmol) in THF dropwise. The reaction was stirred at −78° C. for 1 h, then 6 (10.0 g, 20.1 mmol) in THF (60 ml) was added. The mixture was allowed to warm to rt and stirred for 1 h. TLC indicated the reaction was complete. 2N HCl was added to the mixture and it was extracted with ethyl acetate and washed with water and brine. The organic phase was dried with Na 2 SO 4  followed by filtration and concentration followed by precipitation of the desired product (10 g of 85% purity, 85% chemical yield) with 5% EtOAc/petroleum ether. 
     Step B: Intermediate 126 
     (3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-vinyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-2-one 
     To a solution of 125 (16 g, 32.3 mmol) in 1,4-dioxane (300 mL) and MeOH (30 ml) stirred at rt was added HCl (37%) (150 mL, 32.3 mmol). The reaction mixture was stirred at 60° C. for 3 h. TLC and LCMS indicated the starting material was consumed completely. Water was added and the volatiles were concentrated, a resultant precipitate was filtered and washed with water. The cake was dried to give 126 (14 g, 81%). 
     Step C: Intermediate 127 
     1-tert-butyl 4-((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-vinyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)2,2-dimethylsuccinate 
     To a solution of 10 (14.52 g, 71.8 mmol), DMAP (10.52 g, 86 mmol) and 126 (13 g, 28.7 mmol) in dichloromethane (150 mL) was added EDC (27.5 g, 144 mmol). The mixture was stirred at rt for 2 h, at which time TLC indicated the reaction was complete. The reaction was washed with 2N HCl, and brine. The organics were dried with Na 2 SO 4 , filtered and concentrated. The residue was purified by silica gel chromatography to give 127 (9.5 g, 49%) alogn with an additional 4 g impure product requiring repurification prior to further use. 
     Step D: Intermediates 128 and 129 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-((S)-oxiran-2-yl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (128) and 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-((3a-((R)-oxiran-2-yl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (129) 
     To a solution of 127 (8.5 g, 13.34 mmol) in DCM (90 mL) at rt was added m-CPBA (5.42 g, 26.7 mmol). The reaction mixture was stirred at rt overnight. The mixture was further diluted with DCM 150 ml then washed with saturated NaHCO 3 , water and brine (note for safety reasons this the excess peroxide should be neutralized but was not in this specific procedure). The organic phase was concentrated and purified by silica gel chromatography (EtOAc/PE 5% to 15%) to give 128 (2.0 g, 21%) and 129 (800 mg, 8%) along with 2.4 g of recovered starting material. For 128:  1 H NMR (500 MHz, CHLOROFORM-d) □=4.51 (dd, J=5.4, 11.3 Hz, 1H), 3.26-3.12 (m, 3H), 2.66 (t, J=4.4 Hz, 1H), 2.54 (s, 2H), 2.35 (dd, J=2.7, 4.6 Hz, 1H), 2.18 (d, J=18.3 Hz, 1H), 2.12-1.93 (m, 3H), 1.91-0.74 (m, 52H); LC/MS: m/z calculated 652.5, found 653.3 (M+1) + . For 129:  1 H NMR (500 MHz, CHLOROFORM-d) δ=4.51 (dd, J=5.0, 11.3 Hz, 1H), 3.26-3.07 (m, 2H), 3.00 (dd, J=3.2, 12.6 Hz, 1H), 2.69 (t, J=4.3 Hz, 1H), 2.59-2.41 (m, 3H), 2.21-0.76 (m, 56H); LC/MS: m/z calculated 652.5, found 653.3 (M+1) + . 
     Step E: Intermediate 131 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(3,4-dihydroisoquinolin-2(1H)-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     To a solution of 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-((R)-oxiran-2-yl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (0.2 g, 0.306 mmol) in tert-butanol (3 mL) was added 1,2,3,4-tetrahydroisoquinoline (0.408 g, 3.06 mmol). The reaction mixture was stirred at 100° C. for 5 h. Upon completion and cooling, EtOAc was added and the mixture was washed with 4N HCl, and brine, then dried with Na 2 SO 4 , filtered and concentrated to give 132 (200 mg, 66%) which was used without further purification. LC/MS: m/z calculated 785.6, found 786.5 (M+1) + . 
     Step F: Compound 132 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(3,4-dihydroisoquinolin-2(1H)-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     To a solution of 131 (0.2 g, 0.254 mmol) in DCM (2 mL) was added TFA (1 mL, 12.98 mmol). The reaction mixture was stirred at rt for 1 h then concentrated. The residue was purified by preparative-HPLC to give the title compound (106 mg, 48%) as a TFA salt as white solid.  1 H NMR (500 MHz, METHANOL-d 4 ) δ=7.43-7.15 (m, 4H), 4.66-4.41 (m, 4H), 3.28-3.05 (m, 3H), 3.05-2.83 (m, 2H), 2.73-2.52 (m, 3H), 2.30 (d, J=13.9 Hz, 1H), 2.16-2.03 (m, 1H), 2.00-0.72 (m, 48H); LC/MS: m/z calculated 729.5, found 730.5 (M+1) + . 
     The Examples below were made in a similar manner to the above examples or via combinations or re-ordering of the methods described and/or usage of other methods well known to those skilled in the art. 
     Example 64 
     Compound 133 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(3,4-dihydroisoquinolin-2(1H)-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 729.5, found 730.5 (M+1) + . 
     Example 65 
     Compound 134 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(4-methylpiperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Isolated as a mixture of diastereomers. LC/MS: m/z calculated 682.53, found 683.5 (M+1) + . 
     Example 66 
     Compound 135 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 707.5, found 708.3 (M+1) + . 
     Example 67 
     Compound 136 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((cyclohexylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 695.6, found 696.5 (M+1) + . 
     Example 68 
     Compound 137 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(cyclohexylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 695.5, found 696.5 (M+1) + . 
     Example 69 
     Compound 138 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(benzyl(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 774.6, found 775.5 (M+1) + . 
     Example 70 
     Compound 139 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-((2-(dimethylamino)ethyl)(4-fluorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 834.6, found 835 (M+1) + . 
     Example 71 
     Compound 140 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((2-(dimethylamino)ethyl)(4-fluorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 834.6, found 835 (M+1) + . 
     Example 72 
     Compound 141 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 751.5, found 752.3 (M+1) + . 
     Example 73 
     Compound 142 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 751.5, found 752.3 (M+1) + . 
     Example 74 
     Compound 143 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2,4-dichlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 842.5, found 843.3 (M+1) + . 
     Example 75 
     Compound 144 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 774.6, found 775.4 (M+1) + . 
     Example 76 
     Compound 145 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(4-fluorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 792.5, found 793.5 (M+1) + . 
     Example 77 
     Compound 146 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-fluorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 721.5, found 722.3 (M+1) + . 
     Example 78 
     Compound 147 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2((2-(dimethylamino)ethyl)(4-fluorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 792.5, found 793.5 (M+1) + . 
     Example 79 
     Compound 148 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-fluorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 721.5, found 722.3 (M+1) + . 
     Example 80 
     Compound 149 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2,4-dichlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 842.5, found 843.5 (M+1) + . 
     Example 81 
     Compound 150 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(4-chloro-N-(2-(dimethylamino)ethyl)benzamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     Stereochemistry was tentatively assigned as drawn but not fully confirmed spectroscopically. LC/MS: m/z calculated 822.5, found 823.4 (M+1) + . 
     Example 82 
     Compound 151 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-chlorobenzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 721.5, found 722.3 (M+1) + . 
     Example 83 
     Compound 152 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-benzamidoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 701.5, found 702.5 (M+1) + . 
     Example 84 
     Compound 153 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((((R)-1-(4-Chlorophenyl)ethyl)amino)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 721.5, found 722.3 (M+1) + . 
     Example 85 
     Compound 154 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((N-(4-chlorobenzyl)acetamido)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 749.4, found 750.3 (M+1) + . 
     Example 86 
     Compound 155 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(((4-chlorobenzyl)amino)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 707.4, found 708.3 (M+1) + . 
     Example 87 
     Compound 156 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((N-(4-chlorophenethyl)acetamido)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 763.5, found 764.3 (M+1) + . 
     Example 88 
     Compound 157 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(((4-chlorophenethyl)amino)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 721.5, found 722.3 (M+1) +   
     Example 89 
     Compound 158 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(3-methoxypropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 809.50, found 810.7 (M+1) +   
     Example 90 
     Compound 159 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(3-methoxypropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 809.5, found 810.4 (M+1) +   
     Example 91 
     Compound 160 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-methoxyethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 795.5, found 796.3 (M+1) +   
     Example 92 
     Compound 161 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-methoxyethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 795.5, found 796.3 (M+1) +   
     Example 93 
     Compound 162 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(4-chloro-N-(2-(dimethylamino)ethyl)benzamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 94 
     Compound 163 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 95 
     Compound 164 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)((R)-pyrrolidin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 820.5, found 821.5 (M+1) +   
     Example 96 
     Compound 165 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)((R)-pyrrolidin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 820.5, found 821.5 (M+1) +   
     Example 97 
     Compound 166 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-hydroxyethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 781.5, found 782.5 (M+1) +   
     Example 98 
     Compound 167 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-4-(4-chlorobenzyl)-5,6-dioxomorpholin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 791.4, found 792.5 (M+1) +   
     Example 99 
     Compound 168 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(((5-chloropyridin-2-yl)methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.4, found 739.5 (M+1) +   
     Example 100 
     Compound 169 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-4-(4-chlorobenzyl)-5-oxomorpholin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 777.4, found 778.6 (M+1) +   
     Example 101 
     Compound 170 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-4-(4-chlorobenzyl)-6-oxomorpholin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 777.4, found 777.9 (M+1) +   
     Example 102 
     Compound 171 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-4-(4-chlorobenzyl)-5-oxomorpholin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 777.4, found 777.9 (M+1) +   
     Example 103 
     Compound 172 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((5-chloropyridin-2-yl)methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 737.4, found 739.4 (M+1) +   
     Example 104 
     Compound 173 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-hydroxyethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 781.5, found 782.3 (M+1) +   
     Example 105 
     Compound 174 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-(((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 778.5, found 779.5 (M+1) +   
     Example 106 
     Compound 175 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(((4-chlorophenethyl)(2-(dimethylamino)ethyl)amino)methyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 792.5, found 793.5 (M+1) +   
     Example 107 
     Compound 176 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(N-(4-chlorobenzyl)-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 108 
     Compound 177 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-chlorobenzyl)-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 109 
     Compound 178 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(pyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 834.5, found 835.5 (M+1) +   
     Example 110 
     Compound 179 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-(pyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 834.5, found 835.5 (M+1) +   
     Example 111 
     Compound 180 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(methylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.4 (M+1) +   
     Example 112 
     Compound 181 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2-aminoethyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 780.5, found 781.4 (M+1) +   
     Example 113 
     Compound 182 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 780.5, found 781.4 (M+1) +   
     Example 114 
     Compound 183 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-(methylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 115 
     Compound 184 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(N-methylacetamido)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 836.5, found 837.5 (M+1) +   
     Example 116 
     Compound 185 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-(N-methylacetamido)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 836.5, found 837.5 (M+1) +   
     Example 117 
     Compound 186 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(((S)-1-(5-chloropyridin-2-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.4 (M+1) +   
     Example 118 
     Compound 187 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((S)-1-(5-chloropyridin-2-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.4 (M+1) +   
     Example 119 
     Compound 188 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(phenethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 788.6, found 789.5 (M+1) +   
     Example 120 
     Compound 189 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(cyclohexyl(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.6, found 767.6 (M+1) +   
     Example 121 
     Compound 190 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(cyclohexyl(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.6, found 767.6 (M+1) +   
     Example 122 
     Compound 191 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(((5-chloropyridin-2-yl)methyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 809.5, found 810.5 (M+1) +   
     Example 123 
     Compound 192 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(4-chlorobenzyl)-2-(dimethylamino)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 806.5, found 807.4 (M+1) +   
     Example 124 
     Compound 193 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(2-amino-N-(4-chlorobenzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 778.5, found 779.4 (M+1) +   
     Example 125 
     Compound 194 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(4-chlorobenzyl)-2-(methylamino)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 792.5, found 793.4 (M+1) +   
     Example 126 
     Compound 195 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(((S)-2-(dimethylamino)-1-phenylethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.5 (M+1) +   
     Example 127 
     Compound 196 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((R)-2-(dimethylamino)-1-phenylethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.5 (M+1) +   
     Example 128 
     Compound 197 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((R)-2-(dimethylamino)-1-phenylethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.5 (M+1) +   
     Example 129 
     Compound 198 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(3-(dimethylamino)propyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 130 
     Compound 199 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(3-(dimethylamino)propyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 131 
     Compound 200 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(phenethylamino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 701.5, found 702.5 (M+1) +   
     Example 132 
     Compound 201 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 726.5, found 727.5 (M+1) +   
     Example 133 
     Compound 202 
     2-(((R)-2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((3-carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-hydroxyethyl)(4-chlorobenzyl)amino)-N,N,N-trimethylethanaminium trifluoroacetate. 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 823.5, found 823.5 (M) +   
     Example 134 
     Compound 203 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-4-(4-chlorobenzyl)-6-oxomorpholin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 777.4, found 778.4 (M+1) +   
     Example 135 
     Compound 204 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((S)-2-(dimethylamino)-1-phenylethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.4 (M+1) +   
     Example 136 
     Compound 205 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2-(dimethylamino)ethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 726.6, found 727.5 (M+1) +   
     Example 137 
     Compound 206 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((5-chloropyridin-2-yl)methyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 809.5, found 810.5 (M+1) +   
     Example 138 
     Compound 207 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(isopropylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 655.5, found 656.4 (M+1) +   
     Example 139 
     Compound 208 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(((R)-2-(dimethylamino)-1-phenylethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 744.5, found 745.5 (M+1) +   
     Example 140 
     Compound 209 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(((S)-2-(dimethylamino)-1-phenylethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 744.5, found 745.5 (M+1) +   
     Example 141 
     Compound 210 
     2-(((S)-2-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-((3-carboxy-3-methylbutanoyl)oxy)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-3a-yl)-2-hydroxyethyl)(4-chlorobenzyl)amino)-N,N,N-trimethylethanaminium trifluoroacetate. 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 823.5, found 823.5 (M) +   
     Example 142 
     Compound 211 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-4-(4-chlorobenzyl)-6-oxopiperazin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 776.5, found 777.4 (M+1) +   
     Example 143 
     Compound 212 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-4-(4-chlorobenzyl)-1-methyl-6-oxopiperazin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 790.5, found 791.4 (M+1) +   
     Example 144 
     Compound 213 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-methoxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 145 
     Compound 214 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(piperidin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 146 
     Compound 215 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(piperidin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 147 
     Compound 216 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.5, found 724.4 (M+1) +   
     Example 148 
     Compound 217 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 653.5, found 654.4 (M+1) +   
     Example 149 
     Compound 218 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 653.5, found 654.4 (M+1) +   
     Example 150 
     Compound 219 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-methoxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 822.5, found 823.5 (M+1) +   
     Example 151 
     Compound 220 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 717.5, found 718.5 (M+1) +   
     Example 152 
     Compound 221 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-methoxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 751.5, found 752.5 (M+1) +   
     Example 153 
     Compound 222 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)amino)-1-methoxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 751.5, found 752.5 (M+1) +   
     Example 154 
     Compound 223 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N-(4-fluorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 820.5, found 821.5 (M+1) +   
     Example 155 
     Compound 224 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclohexylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 709.5, found 710.6 (M+1) +   
     Example 156 
     Compound 225 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-4-(4-chlorobenzyl)-1-methyl-5-oxopiperazin-2-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 790.5, found 791.5 (M+1) +   
     Example 157 
     Compound 226 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclohexylmethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 780.6, found 781.5 (M+1) +   
     Example 158 
     Compound 227 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.6, found 739.8 (M+1) +   
     Example 159 
     Compound 228 
     2-(2-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2-oxoethoxy)acetic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 691.4, found 692.5 (M+1) +   
     Example 160 
     Compound 229 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chloro-2-((dimethylamino)methyl)benzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 778.5, found 779.5 (M+1) +   
     Example 161 
     Compound 230 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(4-chloro-2-((dimethylamino)methyl)benzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 820.5, found 821.7 (M+1) +   
     Example 162 
     Compound 231 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(4-chloro-2-((dimethylamino)methyl)benzyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 717.5, found 718.5 (M+1) +   
     Example 163 
     Compound 232 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-benzamido-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 717.5, found 718.5 (M+1) +   
     Example 164 
     Compound 233 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylbenzamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 731.5, found 732.3 (M+1) +   
     Example 165 
     Compound 234 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(N-methylbenzamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 731.5, found 732.5 (M+1) +   
     Example 166 
     Compound 235 
     4-(((3aS,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.7 (M+1) +   
     Example 167 
     Compound 236 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N-(pyridin-2-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 168 
     Compound 237 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(2-(methylamino)-N-(pyridin-2-ylmethyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 169 
     Compound 238 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((4-chloro-2-((dimethylamino)methyl)benzyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 792.5, found 793.5 (M+1) +   
     Example 170 
     Compound 239 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((2-chlorobenzyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 850.5, found 851.5 (M+1) +   
     Example 171 
     Compound 240 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N-benzylacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.5 (M+1) +   
     Example 172 
     Compound 241 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzyl-2-(methylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 774.5, found 775.5 (M+1) +   
     Example 173 
     Compound 242 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(N-benzyl-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 788.5, found 789.5 (M+1) +   
     Example 174 
     Compound 243 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N-(pyridin-2-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 175 
     Compound 244 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(((R)-1-(5-chloropyridin-2-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.5 (M+1) +   
     Example 176 
     Compound 245 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(((R)-1-(5-chloropyridin-2-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.4 (M+1) +   
     Example 177 
     Compound 246 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N—((R)-1-(5-chloropyridin-2-yl)ethyl)-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 837.5, found 838.5 (M+1) +   
     Example 178 
     Compound 247 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2-(dimethylamino)ethyl)(phenethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 788.6, found 789.5 (M+1) +   
     Example 179 
     Compound 248 
     5-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzyl-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-3,3-dimethyl-5-oxopentanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 802.6, found 803.5 (M+1) +   
     Example 180 
     Compound 249 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(2-(dimethylamino)ethyl)cyclohexanecarboxamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.6, found 795.5 (M+1) +   
     Example 181 
     Compound 250 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(cyclohexanecarboxamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.5, found 724.5 (M+1) +   
     Example 182 
     Compound 251 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N—((S)-1-(5-chloropyridin-2-yl)ethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 809.5, found 810.4 (M+1) +   
     Example 183 
     Compound 252 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(N—((S)-1-(5-chloropyridin-2-yl)ethyl)-2-(methylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 823.5, found 824.5 (M+1) +   
     Example 184 
     Compound 253 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N—((S)-1-(5-chloropyridin-2-yl)ethyl)-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 837.5, found 838.5 (M+1) +   
     Example 185 
     Compound 254 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 746.5, found 747.5 (M+1) +   
     Example 186 
     Compound 255 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclobutylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 187 
     Compound 256 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-(benzyl(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 816.6, found 817.5 (M+1) +   
     Example 188 
     Compound 257 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-acetoxy-2-(benzyl(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 816.6, found 817.5 (M+1) +   
     Example 189 
     Compound 258 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(cyclohexanecarboxamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.5, found 724.5 (M+1) +   
     Example 190 
     Compound 259 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((cyclobutylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 191 
     Compound 260 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(N-(2-(dimethylamino)ethyl)cyclohexanecarboxamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.6, found 795.6 (M+1) +   
     Example 192 
     Compound 261 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((cyclobutylmethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.6, found 753.6 (M+1) +   
     Example 193 
     Compound 262 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((1H-pyrazol-3-yl)methyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 764.6, found 765.5 (M+1) +   
     Example 194 
     Compound 263 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 667.5, found 668.5 (M+1) +   
     Example 195 
     Compound 264 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((1S)-1-hydroxy-2-((2-hydroxy-2-phenylethyl)(methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 747.5, found 748.5 (M+1) +   
     Example 196 
     Compound 265 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((2-(pyridin-3-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 718.5, found 719.5 (M+1) +   
     Example 197 
     Compound 266 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((R)-3-hydroxypiperidin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 697.5, found 698.5 (M+1) +   
     Example 198 
     Compound 267 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((3-(2-oxopyrrolidin-1-yl)propyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.5, found 739.5 (M+1) +   
     Example 199 
     Compound 268 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((2-phenoxyethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 733.5, found 734.5 (M+1) +   
     Example 200 
     Compound 269 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(4-(2-hydroxyethyl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 726.5, found 727.5 (M+1) +   
     Example 201 
     Compound 270 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((1S)-2-(2,6-dimethylmorpholino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 711.5, found 712.5 (M+1) +   
     Example 202 
     Compound 271 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-thiomorpholinoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 699.5, found 700.4 (M+1) +   
     Example 203 
     Compound 272 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((3-morpholinopropyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 204 
     Compound 273 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((3-(1H-imidazol-1-yl)propyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 721.5, found 722.5 (M+1) +   
     Example 205 
     Compound 274 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((4-hydroxyphenethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 733.5, found 734.5 (M+1) +   
     Example 206 
     Compound 275 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(cyclopentylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 207 
     Compound 276 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-((cyclohexylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 709.5, found 710.5 (M+1) +   
     Example 208 
     Compound 277 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclobutylmethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.6, found 753.6 (M+1) +   
     Example 209 
     Compound 278 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((1H-pyrazol-4-yl)methyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 764.6, found 765.5 (M+1) +   
     Example 210 
     Compound 279 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((cyclopropylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 667.5, found 668.5 (M+1) +   
     Example 211 
     Compound 280 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(cyclobutylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 667.5, found 668.5 (M+1) +   
     Example 212 
     Compound 281 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(cyclobutylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 667.5, found 668.5 (M+1) +   
     Example 213 
     Compound 282 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((3-(pyrrolidin-1-yl)propyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 214 
     Compound 283 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(4-(pyridin-2-yl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.5, found 760.5 (M+1) +   
     Example 215 
     Compound 284 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(4-methyl-1,4-diazepan-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.5, found 711.5 (M+1) +   
     Example 216 
     Compound 285 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((4-sulfamoylphenethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 796.5, found 797.5 (M+1) +   
     Example 217 
     Compound 286 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(4-carbamoylpiperidin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 218 
     Compound 287 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(4-(2-methoxyethyl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 219 
     Compound 288 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(((R)-2-hydroxy-1-phenylethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 733.5, found 734.5 (M+1) +   
     Example 220 
     Compound 289 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((1S)-1-hydroxy-2-(octahydroisoquinolin-2(1H)-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 735.5, found 736.5 (M+1) +   
     Example 221 
     Compound 290 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((3-(piperidin-1-yl)propyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.5, found 739.5 (M+1) +   
     Example 222 
     Compound 291 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N—((S-1-(pyridin-2-yl)ethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 803.5, found 804.5 (M+1) +   
     Example 223 
     Compound 292 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(cyclopentylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 224 
     Compound 293 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(benzo[d][1,3]dioxol-5-ylamino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 733.5, found 734.5 (M+1) +   
     Example 225 
     Compound 294 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((2-(thiophen-2-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.5, found 724.4 (M+1) +   
     Example 226 
     Compound 295 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(4-(4-methylbenzyl)piperazin-1-yl) ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 786.5, found 787.5 (M+1) +   
     Example 227 
     Compound 296 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(bis(2-methoxyethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 729.5, found 730.5 (M+1) +   
     Example 228 
     Compound 297 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(((1S)-1-hydroxy-2-((2-methylcyclohexyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 709.5, found 710.5 (M+1) +   
     Example 229 
     Compound 298 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(((1S)-1-hydroxy-2-((2-(1-methylpyrrolidin-2-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 230 
     Compound 299 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(((1r,4S)-4-hydroxycyclohexyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 711.5, found 712.4 (M+1) +   
     Example 231 
     Compound 300 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(4-ethylpiperazin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.5, found 711.5 (M+1) +   
     Example 232 
     Compound 301 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(((S)-2-hydroxypropyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 671.5, found 672.4 (M+1) +   
     Example 233 
     Compound 302 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorophenyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.4, found 724.4 (M+1) +   
     Example 234 
     Compound 303 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(isoindolin-2-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 715.5, found 716.5 (M+1) +   
     Example 235 
     Compound 304 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((S)-1-(5-chloropyridin-2-yl)ethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 823.5, found 824.5 (M+1) +   
     Example 236 
     Compound 305 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(((S)-1-(5-chloropyridin-2-yl)ethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 823.5, found 824.5 (M+1) +   
     Example 237 
     Compound 306 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(isoindolin-2-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 715.5, found 716.5 (M+1) +   
     Example 238 
     Compound 307 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((2-(5-chloropyridin-2-yl)propan-2-yl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.5, found 767.5 (M+1) +   
     Example 239 
     Compound 308 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(5-chloropyridin-2-yl)propan-2-yl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.5, found 767.6 (M+1) +   
     Example 240 
     Compound 309 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((S)-1-(pyridin-2-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 718.5, found 719.5 (M+1) +   
     Example 241 
     Compound 310 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 704.5, found 705.5 (M+1) +   
     Example 242 
     Compound 311 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 704.5, found 705.5 (M+1) +   
     Example 243 
     Compound 312 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyridin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 244 
     Compound 313 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(phenylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 689.5, found 690.5 (M+1) +   
     Example 245 
     Compound 314 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((4-methoxyphenyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 719.5, found 720.5 (M+1) +   
     Example 246 
     Compound 315 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((S)-2-(hydroxymethyl)pyrrolidin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 697.5, found 698.5 (M+1) +   
     Example 247 
     Compound 316 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((R)-3-(ethoxycarbonyl)piperidin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 753.5, found 754.5 (M+1) +   
     Example 248 
     Compound 317 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(4-formylpiperazin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.5, found 711.5 (M+1) +   
     Example 249 
     Compound 318 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(4-benzylpiperidin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 771.5, found 772.5 (M+1) +   
     Example 250 
     Compound 319 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(4-acetylpiperazin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 251 
     Compound 320 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(4-(2-morpholinoethyl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 795.6, found 796.5 (M+1) +   
     Example 252 
     Compound 321 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(carboxymethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 253 
     Compound 322 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(((S)-1-(pyridin-2-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 718.5, found 719.5 (M+1) +   
     Example 254 
     Compound 323 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N—((R)-1-(pyridin-2-yl)ethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 803.5, found 804.5 (M+1) +   
     Example 255 
     Compound 324 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(methylamino)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 256 
     Compound 325 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(bis(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 917.5, found 918.5 (M+1) +   
     Example 257 
     Compound 326 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(bis(4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 861.4, found 862.4 (M+1) +   
     Example 258 
     Compound 327 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(pyridin-3-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 718.5, found 719.5 (M+1) +   
     Example 259 
     Compound 328 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((R)-3-hydroxypiperidin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 697.5, found 698.5 (M+1) +   
     Example 260 
     Compound 329 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((4-hydroxyphenethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 733.5, found 734.4 (M+1) +   
     Example 261 
     Compound 330 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(4-(2-hydroxyethyl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 726.5, found 727.5 (M+1) +   
     Example 262 
     Compound 331 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-thiomorpholinoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 699.4, found 700.4 (M+1) +   
     Example 263 
     Compound 332 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(4-(pyridin-2-yl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.5, found 760.5 (M+1) +   
     Example 264 
     Compound 333 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(4-methyl-1,4-diazepan-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.5, found 711.5 (M+1) +   
     Example 265 
     Compound 334 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(4-carbamoylpiperidin-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 266 
     Compound 335 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(4-(2-methoxyethyl)piperazin-1-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 267 
     Compound 336 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((R)-2-hydroxy-1-phenylethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 733.5, found 734.5 (M+1) +   
     Example 268 
     Compound 337 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-((pyrimidin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 705.5, found 706.4 (M+1) +   
     Example 269 
     Compound 338 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((pyrimidin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 705.5, found 706.4 (M+1) +   
     Example 270 
     Compound 339 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzyl-2-(dimethylamino)-2-oxoacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 802.5, found 803.5 (M+1) +   
     Example 271 
     Compound 340 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chloro-2-((dimethylamino)methyl)benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 272 
     Compound 341 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chloro-2-((dimethylamino)methyl)benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 273 
     Compound 342 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chloro-2-((dimethylamino)methyl)benzyl)(methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 808.5, found 809.5 (M+1) +   
     Example 274 
     Compound 343 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-chloro-2-((dimethylamino)methyl)benzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 836.5, found 837.5 (M+1) +   
     Example 275 
     Compound 344 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chloro-2-((dimethylamino)methyl)benzyl)(methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 808.5, found 809.5 (M+1) +   
     Example 276 
     Compound 345 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclopropylmethyl)-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.5 (M+1) +   
     Example 277 
     Compound 346 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(methylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.5 (M+1) +   
     Example 278 
     Compound 347 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(dimethylamino)-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.5 (M+1) +   
     Example 279 
     Compound 348 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(cyclopropylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.5 (M+1) +   
     Example 280 
     Compound 349 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(N-methylacetamido)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.5, found 767.5 (M+1) +   
     Example 281 
     Compound 350 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-amino-2-oxoethyl)(benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.5 (M+1) +   
     Example 282 
     Compound 351 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(dimethylamino)-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 788.5, found 789.5 (M+1) +   
     Example 283 
     Compound 352 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(isobutylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 669.5, found 670.5 (M+1) +   
     Example 284 
     Compound 353 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N-(pyridin-3-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 285 
     Compound 354 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N-(pyridin-3-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 286 
     Compound 355 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(2-(methylamino)-N-(pyridin-3-ylmethyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 287 
     Compound 356 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((3-(trifluoromethyl)benzyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 771.5, found 772.4 (M+1) +   
     Example 288 
     Compound 357 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((pyridin-4-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 704.5, found 705.5 (M+1) +   
     Example 289 
     Compound 358 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(((1R)-1-hydroxy-2-(((tetrahydrofuran-2-yl)methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 697.5, found 698.5 (M+1) +   
     Example 290 
     Compound 359 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(cyclohexyl(methyl) amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 709.5, found 710.5 (M+1) +   
     Example 291 
     Compound 360 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((S)-1-hydroxy-4-methylpentan-2-yl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 713.5, found 714.5 (M+1) +   
     Example 292 
     Compound 361 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((1-methylpiperidin-4-yl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.5, found 711.5 (M+1) +   
     Example 293 
     Compound 362 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzyl-1-carboxyformamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 294 
     Compound 363 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N-benzyl-2-oxoacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 774.5, found 775.5 (M+1) +   
     Example 295 
     Compound 364 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(isobutylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 669.5, found 670.5 (M+1) +   
     Example 296 
     Compound 365 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(phenethylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 717.5, found 718.5 (M+1) +   
     Example 297 
     Compound 366 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyridin-3-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 298 
     Compound 367 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(pyridin-3-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 747.5, found 748.5 (M+1) +   
     Example 299 
     Compound 368 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((1-(5-chloropyrimidin-2-yl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.5, found 766.5 (M+1) +   
     Example 300 
     Compound 369 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N-(pyridin-4-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 301 
     Compound 370 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(2-(methylamino)-N-(pyridin-4-ylmethyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 302 
     Compound 371 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N-(pyridin-4-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 303 
     Compound 372 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyridin-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 304 
     Compound 373 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(methylamino)ethyl)(pyridin-4-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 305 
     Compound 374 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-(2-(cyclohexanecarboxamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 707.5, found 708.5 (M+1) +   
     Example 306 
     Compound 375 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(pyridin-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 747.5, found 748.5 (M+1) +   
     Example 307 
     Compound 376 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(pyridin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 747.5, found 748.5 (M+1) +   
     Example 308 
     Compound 377 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(N-methylacetamido)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 803.5, found 804.5 (M+1) +   
     Example 309 
     Compound 378 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((1-(5-chloropyrimidin-2-yl)cyclopropyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 836.5, found 837.5 (M+1) +   
     Example 310 
     Compound 379 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(methylamino)-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 774.5, found 775.5 (M+1) +   
     Example 311 
     Compound 380 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)-2-oxoethyl)(pyridin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 312 
     Compound 381 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(N-methylacetamido)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 802.5, found 803.5 (M+1) +   
     Example 313 
     Compound 382 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(2-oxopyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 814.5, found 815.5 (M+1) +   
     Example 314 
     Compound 383 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((1H-benzo[d]imidazol-2-yl)methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 743.5, found 744.5 (M+1) +   
     Example 315 
     Compound 384 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-(aminomethyl)-1H-benzo[d]imidazol-1-yl)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 743.5, found 744.5 (M+1) +   
     Example 316 
     Compound 385 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((1-(4-chlorophenyl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 763.5, found 764.5 (M+1) +   
     Example 317 
     Compound 386 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((1-(4-chlorophenyl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 763.5, found 764.5 (M+1) +   
     Example 318 
     Compound 387 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-morpholinoethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 817.6, found 818.5 (M+1) +   
     Example 319 
     Compound 388 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(2-oxopyrrolidin-1-yl)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 815.5, found 816.5 (M+1) +   
     Example 320 
     Compound 389 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-(phenethylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 717.5, found 718.5 (M+1) +   
     Example 321 
     Compound 390 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((thiazol-5-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.4, found 711.4 (M+1) +   
     Example 322 
     Compound 391 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(cyclopentylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 665.5, found 666.5 (M+1) +   
     Example 323 
     Compound 392 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)-2-oxoethyl)(pyridin-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 324 
     Compound 393 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-amino-2-oxoethyl)(pyridin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 325 
     Compound 394 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-((2-hydroxyethyl)amino)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 791.5, found 792.5 (M+1) +   
     Example 326 
     Compound 395 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-((2-methoxyethyl)amino)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 805.6, found 806.5 (M+1) +   
     Example 327 
     Compound 396 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-((tert-butoxycarbonyl)amino)ethyl)(cyclopropylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 810.6, found 811.5 (M+1) +   
     Example 328 
     Compound 397 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-((tert-butoxycarbonyl)(methyl)amino)ethyl)(cyclopropylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 824.6, found 825.5 (M+1) +   
     Example 329 
     Compound 398 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-benzyl-1-carboxyformamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.5, found 760.5 (M+1) +   
     Example 330 
     Compound 399 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(cyclopropylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.5, found 711.5 (M+1) +   
     Example 331 
     Compound 400 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(pyridin-3-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 332 
     Compound 401 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((thiazol-4-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.4, found 711.4 (M+1) +   
     Example 333 
     Compound 402 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((thiazol-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 710.4, found 711.4 (M+1) +   
     Example 334 
     Compound 403 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(thiazol-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 781.5, found 782.5 (M+1) +   
     Example 335 
     Compound 404 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(benzyl(carboxymethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 745.5, found 746.5 (M+1) +   
     Example 336 
     Compound 405 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-(2-(N-methylbenzamido)ethyl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 715.5, found 716.5 (M+1) +   
     Example 337 
     Compound 406 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-benzyl-2-(dimethylamino)-2-oxoacetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 786.5, found 787.5 (M+1) +   
     Example 338 
     Compound 407 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 788.5, found 789.5 (M+1) +   
     Example 339 
     Compound 408 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(2-amino-N-benzyl-2-oxoacetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 758.5, found 759.5 (M+1) +   
     Example 340 
     Compound 409 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(pyridin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 341 
     Compound 410 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(thiazol-5-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 781.5, found 782.5 (M+1) +   
     Example 342 
     Compound 411 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(thiazol-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 781.5, found 782.5 (M+1) +   
     Example 343 
     Compound 412 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(methylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 344 
     Compound 413 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(pyridin-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 345 
     Compound 414 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(cyclopropylmethyl)amino)-1-acetoxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 346 
     Compound 415 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-acetoxy-2-((cyclopropylmethyl)(2-(N-methylacetamido)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 808.6, found 809.5 (M+1) +   
     Example 347 
     Compound 416 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((5-chloropyrimidin-2-yl)methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 739.4, found 740.4 (M+1) +   
     Example 348 
     Compound 417 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyrimidin-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 776.5, found 777.5 (M+1) +   
     Example 349 
     Compound 418 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-(((5-chloropyrimidin-2-yl)methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 739.4, found 740.4 (M+1) +   
     Example 350 
     Compound 419 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(methylamino)ethyl)(pyridin-3-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.5 (M+1) +   
     Example 351 
     Compound 420 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-((R)-2-oxo-3-(pyridin-2-ylmethyl)oxazolidin-5-yl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 730.5, found 731.4 (M+1) +   
     Example 352 
     Compound 421 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((1-(5-chloropyrimidin-2-yl)cyclopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.4, found 766.4 (M+1) +   
     Example 353 
     Compound 422 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2,11-dimethyl-3,10-dioxo-5-(pyridin-3-ylmethyl)-8-oxa-2,5,11-triazadodecan-7-yl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 874.6, found 875.6 (M+1) +   
     Example 354 
     Compound 423 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-(methylamino)-2-oxoethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 775.5, found 776.5 (M+1) +   
     Example 355 
     Compound 424 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)-2-oxoethyl)(pyridin-3-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 789.5, found 790.5 (M+1) +   
     Example 356 
     Compound 425 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N-(pyrimidin-2-ylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 790.5, found 791.5 (M+1) +   
     Example 357 
     Compound 426 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(picolinamido)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.4 (M+1) +   
     Example 358 
     Compound 427 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(1H-imidazol-1-yl)ethyl)(benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 797.5, found 798.5 (M+1) +   
     Example 359 
     Compound 428 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((3-(1H-imidazol-1-yl)propyl)(benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 811.5, found 812.5 (M+1) +   
     Example 360 
     Compound 429 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((3-(1H-imidazol-1-yl)propyl)(benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 811.5, found 812.5 (M+1) +   
     Example 361 
     Compound 430 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-benzyl-2-(dimethylamino)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 772.5, found 773.5 (M+1) +   
     Example 362 
     Compound 431 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(2-(dimethylamino)-N-isopropylacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 363 
     Compound 432 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-isopropyl-2-(methylamino)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 726.5, found 727.5 (M+1) +   
     Example 364 
     Compound 433 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(2-amino-N-isopropylacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 712.5, found 713.5 (M+1) +   
     Example 365 
     Compound 434 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(3-(trifluoromethoxy)benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 858.5, found 859.5 (M+1) +   
     Example 366 
     Compound 435 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(4-(trifluoromethoxy)benzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 858.5, found 859.5 (M+1) +   
     Example 367 
     Compound 436 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(2-(dimethylamino)-N-(pyridin-2-ylmethyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 368 
     Compound 437 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-aminoethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 698.5, found 699.5 (M+1) +   
     Example 369 
     Compound 438 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 370 
     Compound 439 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)-2-oxoethyl)(thiazol-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 795.5, found 796.4 (M+1) +   
     Example 371 
     Compound 440 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-((R)-2-methyl-5-(pyridin-2-ylmethyl)-8,11-dioxa-2,5-diazadodecan-7-yl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 833.6, found 834.5 (M+1) +   
     Example 372 
     Compound 441 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((S)—N-benzyl-3,4-dihydroxybutanamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 805.5, found 806.5 (M+1) +   
     Example 373 
     Compound 442 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-amino-2-oxoethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 712.5, found 713.5 (M+1) +   
     Example 374 
     Compound 443 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)-2-oxoethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 375 
     Compound 444 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(isopropyl(2-(methylamino)-2-oxoethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 726.5, found 727.5 (M+1) +   
     Example 376 
     Compound 445 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((S)-3,4-dihydroxy-N-(pyridin-2-ylmethyl)butanamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 806.5, found 807.5 (M+1) +   
     Example 377 
     Compound 446 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(ethylamino)-2-oxoethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 740.5, found 741.5 (M+1) +   
     Example 378 
     Compound 447 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((carboxymethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 713.5, found 714.5 (M+1) +   
     Example 379 
     Compound 448 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(cyclopropylamino)-2-oxoethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 752.5, found 753.5 (M+1) +   
     Example 380 
     Compound 449 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(((5-chloropyrimidin-2-yl)methyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 810.5, found 811.5 (M+1) +   
     Example 381 
     Compound 450 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclopropylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 709.5, found 710.5 (M+1) +   
     Example 382 
     Compound 451 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((pyridin-2-ylmethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 688.5, found 689.5 (M+1) +   
     Example 383 
     Compound 452 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 681.5, found 682.5 (M+1) +   
     Example 384 
     Compound 453 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclopropylmethyl)-2-(methylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.5, found 739.5 (M+1) +   
     Example 385 
     Compound 454 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzylisobutyramido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 386 
     Compound 455 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(N-benzyl-3-methylbutanamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 787.5, found 788.5 (M+1) +   
     Example 387 
     Compound 456 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzylpropionamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.5, found 760.5 (M+1) +   
     Example 388 
     Compound 457 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((pyridin-3-ylmethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 688.5, found 689.5 (M+1) +   
     Example 389 
     Compound 458 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((pyridin-4-ylmethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 688.5, found 689.5 (M+1) +   
     Example 390 
     Compound 459 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(pyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 764.6, found 765.5 (M+1) +   
     Example 391 
     Compound 460 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-benzylacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 745.5, found 746.5 (M+1) +   
     Example 392 
     Compound 461 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-morpholinoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 816.6, found 817.5 (M+1) +   
     Example 393 
     Compound 462 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N, 3-dimethylbutanamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 711.5, found 712.5 (M+1) +   
     Example 394 
     Compound 463 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclopropylmethyl)-2-(2-oxopyrrolidin-1-yl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 792.5, found 793.5 (M+1) +   
     Example 395 
     Compound 464 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-(isobutyryloxy)-2-(methylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 697.5, found 698.5 (M+1) +   
     Example 396 
     Compound 465 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((cyclobutylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 665.5, found 666.5 (M+1) +   
     Example 397 
     Compound 466 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((cyclopropylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 651.5, found 652.5 (M+1) +   
     Example 398 
     Compound 467 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclobutylmethyl)-2-(dimethylamino)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.5, found 767.5 (M+1) +   
     Example 399 
     Compound 468 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((cyclobutylmethyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 736.6, found 737.5 (M+1) +   
     Example 400 
     Compound 469 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((pyrimidin-4-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 705.5, found 706.5 (M+1) +   
     Example 401 
     Compound 470 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclobutylmethyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.5, found 724.5 (M+1) +   
     Example 402 
     Compound 471 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-((4-chlorobenzoyl)oxy)-2-(methylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.4, found 766.4 (M+1) +   
     Example 403 
     Compound 472 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-(benzoyloxy)-2-((2-hydroxyethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.4 (M+1) +   
     Example 404 
     Compound 473 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-((R)-2-(methylamino)-1-(2-phenylacetoxy)ethyl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 745.5, found 746.5 (M+1) +   
     Example 405 
     Compound 474 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylacetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 669.5, found 670.4 (M+1) +   
     Example 406 
     Compound 475 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(4-chloro-N-methylbenzamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.4, found 766.4 (M+1) +   
     Example 407 
     Compound 476 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methyl-2-phenylacetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 745.5, found 746.5 (M+1) +   
     Example 408 
     Compound 477 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-(2-hydroxyethyl)benzamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 761.5, found 762.4 (M+1) +   
     Example 409 
     Compound 478 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((pyrimidin-5-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 705.5, found 706.5 (M+1) +   
     Example 410 
     Compound 479 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-((cyclobutylmethyl)amino)-2-oxoethyl) (isopropyl) amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 780.6, found 781.6 (M+1) +   
     Example 411 
     Compound 480 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylisobutyramido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 697.5, found 698.5 (M+1) +   
     Example 412 
     Compound 481 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylcyclopentanecarboxamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 723.5, found 724.5 (M+1) +   
     Example 413 
     Compound 482 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylcyclohexanecarboxamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 737.5, found 738.5 (M+1) +   
     Example 414 
     Compound 483 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((cyclopropylmethyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 722.6, found 723.6 (M+1) +   
     Example 415 
     Compound 484 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(cyclopentyl(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 736.6, found 737.5 (M+1) +   
     Example 416 
     Compound 485 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyrimidin-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 776.5, found 777.5 (M+1) +   
     Example 417 
     Compound 486 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((S)-2-(benzyl(2-(2-oxopyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 814.5, found 815.5 (M+1) +   
     Example 418 
     Compound 487 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylpicolinamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 732.5, found 733.5 (M+1) +   
     Example 419 
     Compound 488 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-chlorobenzyl)-2-hydroxyacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 795.4, found 796.3 (M+1) +   
     Example 420 
     Compound 489 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylisonicotinamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 732.5, found 733.5 (M+1) +   
     Example 421 
     Compound 490 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(N-methylnicotinamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 732.5, found 733.4 (M+1) +   
     Example 422 
     Compound 491 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-chlorobenzyl)-2-methoxyacetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 809.5, found 810.3 (M+1) +   
     Example 423 
     Compound 492 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(4-chlorobenzyl)isobutyramido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 807.5, found 808.5 (M+1) +   
     Example 424 
     Compound 493 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(N-(2-(dimethylamino)ethyl)-5-methyl-1,3,4-oxadiazole-2-carboxamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 425 
     Compound 494 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((pyrimidin-2-ylmethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 689.5, found 690.4 (M+1) +   
     Example 427 
     Compound 496 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-sulfoethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 801.4, found 802.2 (M+1) +   
     Example 428 
     Compound 497 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((pyrimidin-4-ylmethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 689.5, found 690.4 (M+1) +   
     Example 429 
     Compound 498 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)-2-oxoethyl)(pyridin-3-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 430 
     Compound 499 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)-2-oxoethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 431 
     Compound 500 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyrimidin-5-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 776.5, found 777.4 (M+1) +   
     Example 433 
     Compound 502 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(((R)-1-(pyridin-2-3/1)ethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.3 (M+1) +   
     Example 434 
     Compound 503 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclobutylmethyl)-2-(2-oxopyrrolidin-1-yl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 806.5, found 806.9 (M+1) +   
     Example 435 
     Compound 504 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclobutylmethyl)(2-(methylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.5, found 739.5 (M+1) +   
     Example 436 
     Compound 505 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(oxazol-2-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.5, found 765.9 (M+1) +   
     Example 437 
     Compound 506 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(1-oxoisoindolin-2-yl)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 729.5, found 729.9 (M+1) +   
     Example 438 
     Compound 507 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(cyclobutylamino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 651.5, found 652.0 (M+1) +   
     Example 439 
     Compound 508 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(cyclobutyl(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 722.6, found 723.0 (M+1) +   
     Example 440 
     Compound 509 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-acetamidoethyl)(cyclobutylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.5, found 766.9 (M+1) +   
     Example 441 
     Compound 510 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(2-(dimethylamino)-N-(pyridin-3-ylmethyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 442 
     Compound 511 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.6, found 760.5 (M+1) +   
     Example 443 
     Compound 512 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclobutylmethyl)(2-(dimethylamino)-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 766.5, found 767.5 (M+1) +   
     Example 444 
     Compound 513 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(pyridin-3-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.6, found 760.5 (M+1) +   
     Example 445 
     Compound 514 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(pyridin-4-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 759.6, found 760.5 (M+1) +   
     Example 446 
     Compound 515 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(2-(dimethylamino)-N-(pyridin-4-ylmethyl)acetamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 447 
     Compound 516 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclobutylmethyl)(2-(N-methylacetamido)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 780.6, found 781.4 (M+1) +   
     Example 448 
     Compound 517 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((R)-1-(pyrimidin-4-3/1)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 719.5, found 720.4 (M+1) +   
     Example 449 
     Compound 518 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((azetidin-3-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 666.5, found 667.4 (M+1) +   
     Example 450 
     Compound 519 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(cyclopropylmethyl)-2-(pyrrolidin-1-yl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 778.5, found 779.5 (M+1) +   
     Example 451 
     Compound 520 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(((R)-1-(pyridin-4-yl)ethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.5 (M+1) +   
     Example 452 
     Compound 521 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((azetidin-3-ylmethyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 737.6, found 738.7 (M+1) +   
     Example 454 
     Compound 523 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-morpholinoethyl)(pyridin-3-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 817.6, found 818.5 (M+1) +   
     Example 455 
     Compound 524 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)((R)-1-(pyridin-2-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.5 (M+1) +   
     Example 456 
     Compound 525 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)((5-methyl-1,3,4-oxadiazol-2-yl)methyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 780.6, found 781.5 (M+1) +   
     Example 457 
     Compound 526 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(((R)-1-(pyridin-3-3/1)ethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.5 (M+1) +   
     Example 458 
     Compound 527 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((1-acetylazetidin-3-yl)methyl)(2-(dimethylamino)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 779.6, found 780.7 (M+1) +   
     Example 459 
     Compound 528 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(pyrimidin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 760.5, found 761.7 (M+1) +   
     Example 460 
     Compound 529 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((S)-1-(pyrimidin-5-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 719.5, found 720.5 (M+1) +   
     Example 461 
     Compound 530 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-morpholino-2-oxoethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 831.5, found 832.7 (M+1) +   
     Example 462 
     Compound 531 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)((R)-1-(pyridin-4-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.7 (M+1) +   
     Example 463 
     Compound 532 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-morpholino-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 830.5, found 831.5 (M+1) +   
     Example 464 
     Compound 533 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((cyclopentylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 679.5, found 680.7 (M+1) +   
     Example 465 
     Compound 534 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(((1-acetylazetidin-3-yl)methyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 708.5, found 709.7 (M+1) +   
     Example 466 
     Compound 535 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-((2-((2-methoxyethyl)(methyl)amino)ethyl)(pyridin-2-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 819.6, found 820.5 (M+1) +   
     Example 467 
     Compound 536 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(2-(dimethylamino)ethyl)benzamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 772.5, found 773.5 (M+1) +   
     Example 468 
     Compound 537 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((S)-1-(pyrimidin-4-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 719.5, found 720.5 (M+1) +   
     Example 469 
     Compound 538 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((1-(pyridin-2-yl)cyclopropyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 714.5, found 715.5 (M+1) +   
     Example 471 
     Compound 540 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(piperidin-1-yl)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 665.5, found 666.5 (M+1) +   
     Example 472 
     Compound 541 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-(((S)-1-(pyridin-3-yl)ethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.4 (M+1) +   
     Example 473 
     Compound 542 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)((S)-1-(pyridin-3-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.7 (M+1) +   
     Example 474 
     Compound 543 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)((R)-1-(pyridin-3-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.7 (M+1) +   
     Example 475 
     Compound 544 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-(((R)-1-(pyrimidin-5-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 719.5, found 719.8 (M+1) +   
     Example 476 
     Compound 545 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(N-(2-chlorobenzyl)acetamido)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 779.4, found 780.3 (M+1) +   
     Example 477 
     Compound 546 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(2-oxopyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 848.5, found 849.5 (M+1) +   
     Example 478 
     Compound 547 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-3a-(2-((oxetan-3-ylmethyl)amino)ethyl)-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 667.5, found 668.4 (M+1) +   
     Example 479 
     Compound 548 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((8)-1-(pyridin-2-yl)ethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.5 (M+1) +   
     Example 480 
     Compound 549 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((8)-1-(pyridin-4-yl)ethyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 702.5, found 703.4 (M+1) +   
     Example 481 
     Compound 550 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)((R)-1-(pyrimidin-4-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 790.6, found 791.5 (M+1) +   
     Example 482 
     Compound 551 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(1-(pyridin-2-yl)cyclopropyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 785.6, found 786.5 (M+1) +   
     Example 483 
     Compound 552 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((2-(pyridin-2-3/1) propan-2-yl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 716.5, found 717.5 (M+1) +   
     Example 484 
     Compound 553 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(oxetan-3-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 738.5, found 739.5 (M+1) +   
     Example 485 
     Compound 554 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)((S)-1-(pyridin-2-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.5 (M+1) +   
     Example 486 
     Compound 555 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)((S)-1-(pyridin-4-yl)ethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.5 (M+1) +   
     Example 487 
     Compound 556 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((1-(pyrimidin-2-yl)cyclopropyl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 715.5, found 716.5 (M+1) +   
     Example 488 
     Compound 557 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(1-(pyrimidin-2-yl)cyclopropyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 786.6, found 787.5 (M+1) +   
     Example 489 
     Compound 558 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-(N-(2-(dimethylamino)ethyl)picolinamido)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.5, found 774.5 (M+1) +   
     Example 490 
     Compound 559 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((2-(pyridin-3-yl)propan-2-yl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 716.5, found 717.5 (M+1) +   
     Example 491 
     Compound 560 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3a-(2-((2-(pyridin-4-yl)propan-2-yl)amino)ethyl)-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 716.5, found 717.5 (M+1) +   
     Example 492 
     Compound 561 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-(benzyl(2-(4-methylpiperazin-1-yl)-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 843.6, found 844.5 (M+1) +   
     Example 493 
     Compound 562 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(oxazol-4-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.5, found 766.5 (M+1) +   
     Example 494 
     Compound 563 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)ethyl)(2-(pyridin-2-yl)propan-2-yl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 787.6, found 788.5 (M+1) +   
     Example 495 
     Compound 564 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-(2-((2-(dimethylamino)-2-oxoethyl)(pyridin-4-ylmethyl)amino)ethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 773.6, found 774.5 (M+1) +   
     Example 496 
     Compound 565 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(oxazol-5-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 765.5, found 766.5 (M+1) +   
     Example 497 
     Compound 46 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Step A: Intermediate 566 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysene-3a-carbaldehyde. 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-Formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (50 g) placed in a round bottomed flask with methanol (500 ml, 10 vol) and DCM (“dichloromethane”—200 ml, 4 vol) and stirred at ambient temp while adding solid ZrCl 4  (23.4 g, 1 equiv.) in portions. 
     The reaction was warmed to approximately 60° C. and maintained for a total of 16 hours. The reaction was monitored for completion on HPLC (starting material and product formed an acetal derivative during the reaction). Once complete, the reaction was treated with water (5 equiv.) and maintained at 60 degrees for approximately 30 minutes. 
     Next, the reaction was cooled and evaporated under vacuum at a bath temp of approximately 40° C. to 200 ml (4 volumes). Ethyl acetate (500 ml, 10 vol) was added and reaction was then treated with 1N HCl (250 ml, 5 vol). The reaction was then mixed thoroughly and the layers were allowed to separate. The lower aqueous layer was drained off and back-washed with fresh ethyl acetate (100 ml, 2 vol). 
     The combined organic layer was washed with 1N HCl (250 ml, 5 vol) and evaporated under vacuum at a bath temp of 40° C. to 200 ml (4 vol) and then treated with acetonitrile (500 ml, 10 vol). The reaction was then evaporated to a final volume of approximately 150 ml (3 vol). Acetonitrile (500 ml, 10 vol) was then added and the reaction was warmed to a 60° C. forming solution. Next, was slowly added 3N HCl (100 ml, 2 vol), which resulted in precipitate formation. (see note 2). The reaction was then allowed to cool to ambient temperature and then it was filtered. The filtrated solid was next rinsed with acetonitrile (2 vol) and dried in vacuo to give a weight of 33.0 g, 72% yield of (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysene-3a-carbaldehyde. 
     Note 1: The isolated solids were contaminated with a small amount of dimethyl acetal of product, which required reslurry in hot acetonitrile/3N HCl to complete the hydrolysis and resulted in loss of product (27 g recovered on re-treatment). It was determined that in process monitoring of reaction slurries during final hydrolysis require filtration of reaction aliquot in order to observe remaining dimethyl acetal. Dissolution of crude acetonitrile/aqueous HCl reaction solution for HPLC monitoring results in artificially low observed levels of dimethyl acetal due to hydrolysis in sample prep. 
     Note 2: Preferred procedure would be to add a portion of the 3N HCl (0.5 vol) and maintain reaction in solution at approximately 60° C. until dimethyl acetal hydrolysis is complete followed by addition of remainder (1.5 vol) of the 3N HCl and cooling to ambient temperature for filtration. Expected yield is 72%. 
     Step B: Intermediate 567 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-Hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysene-3a-carbaldehyde (26.9 g, 1 equiv), 4-(tert-butoxy)-3,3-dimethyl-4-oxobutanoic acid (14.96 g, 1.25 equiv) and THF (538 ml, 20 vol) were charged to a flask. Benzoyl chloride (10.4 g, 1.25 equiv) was added followed by slow addition of triethylamine (14.97 g, 2.5 equiv) forming a thick slurry. DMAP (0.1.81 g, 0.25 equiv) was added and the reaction was allowed to stir at ambient temperature for approximately 18 hours until complete by HPLC. Reaction was then charged with ethyl acetate (538 mL, 20 vol) and water (269 ml, 10 vol), stirred well and allowed to settle. Aqueous phase was removed and the organic phase washed with saturated sodium bicarbonate solution (2×10 vol) followed by water (269 mL, 10 vol). The reaction solution evaporated under vacuum to approximately 4 volumes to produce solids. Additional hexanes (269 ml, 10 vol) was added and reaction heated back to reflux to nearly form solution and slowly cooled to ambient temperature (seeding with desired product) and let stir for 15 minutes then cooled to 0° C. for approximately two hours and filtered solid. Washed solid with hexanes (2×10 vol) and dried at 50° C. under vacuum to a weight of 32.15 g, 85% yield of 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate. 
     Step C: Intermediate 569 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)2,2-dimethylsuccinate 
     The reaction was charged with 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (15.75 g, 1 equiv), tert-butanol (157 ml, 10 vol) and toluene (47 ml, 3 vol) and stirred at ambient temperature. The ligand (N1,N2-bis(1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)ethane-1,2-diamine, derived from (S)-camphor, 0.492 g, 0.06 equiv) and copper (II) acetate mono hydrate (0.246 g, 0.05 equiv) were added in a single portion and reaction allowed to stir at ambient temperature for at least 2.5 hours (see note 1). The reaction is charged with nitromethane (7.52 g, 5 equiv) and Hunig&#39;s base (“N,N-diisopropylethylamine”—3.82 g, 1.2 equiv) and allowed to stir at ambient temperature for approximately 5 days. (See note 2). Reaction concentrated under vacuum at bath temp of no more than 35° C. to approximately 2 vols. (See note 3). Reaction charged with toluene (236 ml, 15 vols) and concentrated under vacuum again to approx 3 vols. Additional toluene (95 ml, 3 vol) was added to achieve a final reaction volume of approx 6 vols. Heptane (158 ml, 10 vols) was added slowly of to solution. Product began to crystallize out of solution upon seeding. Reaction was allowed to stir at ambient temperature for one to two hours and solids collected by filtration and washed with 10 vols of heptane. Solids were dried to a weight of 14.3 g (83% yield). Purity of isolated solid is typically 90-95% with a 98/2 diastereomeric ratio favoring 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate as the major diastereomer. 
     Step D: Intermediate 570 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (12.9 g, 18.4 mmol) was placed in 1-L retreat curve impeller reactor and then added 22 vol of 90% THF-HOAc and stirred to dissolve at ambient temp. The reactor was purged 3× with nitrogen and then a slurry of water was added and then washed (pH 8-9) RaNi catalyst (50% water, added 2.6 ml catalyst slurry, 1.3 g RaNi calculated) and then placed under hydrogen at 0.3 bar pressure for 3 hours and then raised the pressure to 2 bar and allowed to stir for 18 hours. Reaction was approximately 20% complete at this time. The temperature was raised to 35 deg C. and then the reaction was stirred for another 7 hours and sampled. The reaction was approximately 31% complete at this time. Reaction pressure was increased to 4 bar hydrogen and was allow to continue stirring at 35 deg for 20 hours. Reaction is 75% complete at this time and no change in impurity profile. Added another 0.15 wt of RaNi (3.0 ml of 50% slurry) to bring total RaNi loading up to 25 wt % and the reaction was allowed to stir at 35 deg under 4 bar Hydrogen for another 24 hours. The reaction was then determined to be complete by HPLC. 
     The reaction was then filtered through a 4 micron in-line filter, rinsing with THF (50 ml). Evaporated to approximately 3 vol and added ethyl acetate (195 ml, 15 vol) and washed with 1N NaOH solution (3×10 vol) and verified final wash to be basic pH. (Note: addition of 0.5 vol saturated NaCl solution was required on second and third washes to obtain efficient separation). Washed with saturated sodium chloride solution (5 vol) and evaporated to a solid foam that was azeotroped with 2×5 vol dichloromethane in order to obtain a solid foam. Dried on high vacuum pump to weight of 11.1 g (90% yield). 
     Recrystallized above sample (10.5 g) by dissolving in methanol (147 ml, 14 volumes) at reflux and then cooling with seeding to ambient temperature and allowed to stir 30 minutes). Mixture was further cooled to 15 degrees and filtered. Solids collected by filtration and washed with methanol (1 volume) and dried at 50 degrees in vacuo to a weight of 7.5 g (71% yield on recrystallization) of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate. 
     Step E and F: Compound 46 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-Amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate and p-Cl-benzaldehyde (1.1 equiv) were placed in a round bottomed flask with EtOH (10 vol) and added Na 2 CO 3  powder (2.5 equiv) and allowed to stir overnight (16 hrs). The next day, the reaction slurry was cooled to approximately 10 degrees and added NaBH 4  to the reaction in a single portion and allowed reaction to stir while slowly warming to ambient temperature. Obtained HPLC after 45 minutes showing essentially complete conversion to desired product. p-Cl-benzyl alcohol present as well (using 220 nm wavelength). The reaction was then diluted with methylene chloride (30 ml, 10 vol) and water (30 ml, 10 vol) and the layers were allowed to separate. The DCM layer was then washed with additional water (10 vol) and evaporated to minimum volume on rotovap and added back DCM (10 vol) and treated with trifluoroacetic acid (6 ml, 2 vol) and the reaction was allowed to stir at ambient temperature. The reaction was almost complete after one hour, but it was left to stir overnight. After 18 hours reaction complete by HPLC and was evaporated to an oil. 
     Dissolved with warming to approximately 45 degrees in ethyl acetate (60 ml, 20 vol) and washed with NaHCO 3  solution (2×10 vol) to remove residual TFA. Then, washed with ethyl acetate and with half-saturated NaCl solution and evaporated to dryness and azeotroped dry with isopropanol (2×5 vol). Dissolved in isopropanol (5 vol) and diluted with acetonitrile to cloud point (10 vol) and then allowed to crystallize overnight. Cloudy mixture, but no filterable solids present. Meanwhile the test crystallization from ethyl acetate-heptane of the original isolated TFA salt solution had produced nice solids. 
     The entire reaction was then evaporated to dryness and azeotroped several portions of ethyl acetate on rotovap (3×10 vol) to remove all isopropanol and acetonitrile. Reaction dissolved with warming to reflux in ethyl acetate (30 ml, 10 vol). Added one equivalent trifluoroacetic acid (0.51 g) and slowly diluted with heptane to cloud point (15 ml, 3 volumes). Seeded with test crystallization seeds from above. Nice solids resulted almost immediately. Stirred with cooling to ambient temperature. Filtered solids and washed with heptane (15 ml, 3 vol). Dried on funnel to weight of 2.55 g, 67% yield of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid. 
     Alternative Procedure 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-Amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (7 g, 1 equiv.) was placed in a round bottom flask and added MgSO 4  powder (2.5 equiv) and Et 3 N (0.8 equiv) and p-Chlorobenzaldehyde and added MeOH (10 vol) and allowed to stir overnight (16 hrs). After this time an aliquot (0.5 ml) of the white slurry was removed and treated with approximately 25 mg NaBH 4  and allowed to stir for 5 minutes. Obtained HPLC of this aliquot indicating complete consumption of the amine starting material. The reaction mixture was cooled to 0 degrees on ice-brine bath and treated with solid NaBH 4  in a single portion (475 mg, 1.2 equiv) and allowed to stir at 0 degrees for approximately 45 minutes. HPLC after this time shows reaction is complete. The reaction was warmed to ambient temperature briefly and filtered through a celite bed. The solid was rinsed with additional methanol (35 ml, 5 vol) and then dichloromethane (70 ml, 10 vol). The filtrate/rinse was then treated with water (70 ml, 10 vol) and shaken in separatory funnel. The upper aqueous was washed well with additional dichloromethane (3×5 vol) and then the combined organic layers were washed with brine (10 vol). The organic phase was evaporated to a solid foam and azeotroped with dichloromethane. Dissolved in dichloromethane (70 ml, 10 vol) and treated with trifluoroacetic acid (14 ml, 2 vol) and allowed to stir at ambient temperature. The reaction almost complete in approximately 2 hours. 
     The reaction was allowed to sit over a weekend at which point the reaction was complete. The reaction was allowed to evaporate to a minimum volume and then ethyl acetate (10 vol) was added and evaporated to a minimum volume again. Repeated this evaporating to minimum volume and then dissolved in total of 10 volume ethyl acetate. Heated to near reflux and diluted with heptane (5 vol) and stirred while cooling to ambient temperature while solids were formed. Stirred for 30 minutes and filtered. Rinsed with heptane (3 vol) and dried to a weight of 4.85 g, 63% yield of 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid over two steps. 
     Example 498 
     Compound 51 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Step A: Intermediate 566 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysene-3a-carbaldehyde 
     The compound (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (400 g, 0.81 mol) was placed in a flask with methanol (4 L) and DCM (1.6 L) and stirred at ambient temp while adding solid ZrCl 4  (225.2 g, 0.97 mol) in portions. The reaction was warmed to approximately 45-55° C. and maintained for a total of 30-36 hours. The reaction was monitored for completion by HPLC. 
     Once complete, the reaction was then treated with water (120 mL) and maintained at 45-55° C. for approximately 30-60 minutes. The reaction was then cooled and evaporated under vacuum (at bath temp approximately 40° C.) to 1200 mL. Dichloromethane (4 L) was added and reaction was treated with 1N HCl (2 L), mixed thoroughly and the layers were allowed to separate. Afterwards, the upper aqueous layer was washed with fresh dichloromethane (800 mL). Next, the combined organic layer was washed with 1N HCl (2 L) and evaporated under vacuum (at bath temp of 40° C.) to 800 mL and treated with acetonitrile (4 L). 
     The reaction was then evaporated (at bath temp of 40° C.) to a final volume of approximately 800 mL. Acetonitrile (3.2 L) was added and the reaction was warmed to 50-60° C., thus forming a solution. Next was slowly added, 3N HCl (100 mL), which resulted in precipitate formation and which was maintained as a reaction in solution at approximately 50-60° C. until dimethyl acetal hydrolysis was complete followed by addition of water (4.8 L). 
     The reaction was allowed to cool to 0-10° C. and then the mixture was filtered and the resultant solid was rinsed with acetonitrile/water (1/1,800 mL). The solid was then slurried in 10 volumes of heptane at 85-100° C. for 1-3 hours. The slurry was then cooled to ambient temperature and filtered. Washed the cake with 800 mL heptane then dried in vacuo at 40-50° C. to the 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 286 g. Yield is 72.6% corrected by assay. 
       1 H NMR (400 MHz, CDCl3) δ=9.33 (s, 1H), 3.20-3.28 (m, 2H), 2.57 (d, J=1H), 2.36-2.42 (m, 2H), 2.04-2.08 (m, 2H), 1.86-1.90 (m, 2H), 1.75-1.78 (m, 1H), 1.23-1.66 (m, 35H); LC/MS: m/z calculated 454.3, found 455.4 (M+1) +   
     Step B: Intermediate 567 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     The starting material (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysene-3a-carbaldehyde (32.4 g, 71 mmol), 4-(tert-butoxy)-3,3-dimethyl-4-oxobutanoic acid (18 g, 89 mmol) and THF (648 mL) were charged to a flask. Benzoyl chloride (12.5 g, 89 mmol) was added followed by slow addition of triethyl amine (18 g, 178 mmol) forming a thick slurry. DMAP (2.17 g, 18 mmol) was added and the reaction was allowed to stir at ambient temperature for approximately 24-30 hours until complete by HPLC. The reaction was then charged with ethyl acetate (648 mL) and water (324 mL), stirred well and allowed to settle. The aqueous phase was removed and the organic phase washed with saturated sodium bicarbonate solution (2×324 mL) followed by water (324 mL). 
     The reaction solution was then evaporated under vacuum to approximately 129 mL in order to produce solids. Additional heptane (324 mL) was added and the reaction heated back to reflux to a nearly formed solution and slowly cooled to ambient temperature and was stirred for 15 minutes then cooled to 0° C. for approximately two hours and the solids were filtered. The solids were then washed with heptane (2×324 mL) and dried at 50° C. under vacuum to give the desired compound as an off-white solid 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)2,2-dimethylsuccinate 36.8 g. yield is 80%. 1H NMR (400 MHz, CDCl3) δ=9.24 (s, 1H), 4.41-4.45 (m, 1H), 3.19-3.20 (m, 1H), 2.33-2.48 (m, 5H), 0.96-1.60 (m, 37H), 0.77-0.87 (m, 17H); LC/MS: m/z calculated 638.4, found 639.4 (M+1) + . 
     Step C: Intermediate 569 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)2,2-dimethylsuccinate 
     The reaction was charged with of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (50 g, 78 mmol), tert-butanol (500 mL) and toluene (150 mL) and the temperature was adjusted to 20-25° C. The ligand (N1,N2-bis(1,7,7-trimethylbicyclo [2.2.1] heptan-2-yl)ethane-1,2-diamine (1.56 g, 4.7 mmol) and Copper(I) acetate (0.48 g, 3.9 mmol) were added in a single portion and the reaction was then stirred at 20-25° C. for 3-5 hrs. 
     The reaction was next allowed to cool to 7-13° C. The reaction was then charged with nitromethane (33 g, 0.55 mmol) and N,N-diisopropyl ethylamine (12.1 g, 94 mmol) and allowed to stir at 7-13° C. for approximately 30 hrs. The reaction was again charged with nitromethane (17 g, 0.3 mmol) and allowed to stir at 7-13° C. for approximately 40 hrs. The reaction was yet again charged with nitromethane (17 g, 0.3 mmol) and allowed to stir at 7-13° C. for approximately 20 hrs. 
     Next, the reaction was charged with MTBE (750 mL) and a 15% ammonium chloride solution (300 mL), allowed to stir for 30 min, and then separated into two phases. The organic phase was washed with water (250 mL) followed by brine (200 mL). The organic phase was then concentrated to 100 mL and MTBE (25 mL) was added. Next, n-heptane (500 mL) was slowly added and then stirred at 10-20° C. for 2 hrs. The reaction was then filtered and washed with n-heptane, and then dried under vacuum at 50-55° C. to give 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate as off-white solid 41.6 g. Yield is 75.9%, 1 H NMR (400 MHz, CDCl3) δ=4.85 (bd, 1H), 4.51-4.55 (dd, 1H), 4.08-4.20 (m, 2H), 3.15-3.22 (m, 1H), 2.35-2.83 (m, 6H), 0.80-2.03 (m, 54H); LC/MS: m/z calculated 699.4, found 700.4 (M+1) + . 
     Step D: Intermediate 570 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)1-tert-butyl 2,2-dimethylsuccinate 
     To 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (30 g, 42.6 mmol) in THF (600 mL) and AcOH (30 mL) was added Raney Ni (30 g, 5%) under N 2  at room temperature. The reaction was stirred at 45-55° C. under hydrogen gas (0.30-0.40 MPa) for 10-12 hours. The reaction mixture was filtered through a filter aid, washing with THF [2×90 mL] and the filtrate was adjusted to a pH of 7-8 with 5% K 2 CO 3  aqueous. 
     Next, ethyl acetate (450 mL) was added to the reaction mixture and the two phases were separated. The aqueous layer was extracted with ethyl acetate (150 mL). The combined organic phase was washed two times with 7.5% NaCl aqueous (300 mL) and then washed with 17% NaCl aqueous (300 mL). The organic phase was concentrated under vacuum to give 25.7 g of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate. Yield is 92%.  1 H NMR (400 MHz, DMSO-d 6 ) δ=4.40-4.44 (dd, 1H), 3.79-3.80 (bd, 1H), 3.59-3.62 (M, 2H), 3.12-3.17 (m, 2H), 2.60-2.75 (m, 1H), 2.22-2.34 (m, 2H), 2.19-2.22 (m, 2H), 1.37-1.84 (m, 25H), 1.08-1.11 (m, 17H), 0.81-0.91 (m, 13H); LC/MS: m/z calculated 669.5, found 670.4 (M+1) +   
     Step E: Intermediate 571 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)2,2-dimethylsuccinate 
     To a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate in THF (5 g, 7.6 mmol in 100 mL THF) was added 4-chlorobenzaldehyde (0.96 g, 6.84 mmol) and then stirred at −2-2° C. for 0.5 hours. Next, NaHB(OAc) 3  (3.53 g, 16.7 mmol) was added to the reaction and stirred at −2-2° C. for 0.5 hours. The reaction was then warmed to 3-7° C. and again stirred at 3-7° C. for 10-15 hours and then stirred at 8-12° C. for 5-8 hours. 
     The reaction was quenched by saturated NI-1 4 Cl (aqueous) (50 mL) and water (20 mL) and then stirred at 10-20° C. for 0.5 hours. The reaction was then extracted with MTBE (75 mL) and the aqueous layer was extracted with MTBE (25 mL). The combined organic phase was washed with water (25 mL) and the pH adjusted to 7-8 with 5% K 2 CO 3  aqueous. The organic phase was separated and washed two times with 7.5% NaCl aqueous (50 mL) and then washed with 20% NaCl aqueous (50 mL). The organic phase was concentrated under vacuum again to approx 20 mL. Additional DCM (60 mL) was added and concentrated under vacuum to approx 20 mL. Additional DCM (60 mL) was again added and concentrated under vacuum to approx 20 mL. Additional DCM (60 mL) was again added and concentrated under vacuum to approx 20 mL. Then, 50 mL MeOH was charged and concentrated under vacuum again to approx 20 mL. An additional 50 mL MeOH was charged to give a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate in MeOH. This mixture was used in the next step directly. 1HNMR (400 MHz, DMSO-d6) δ=0.81-1.78(m,49H),1.91(s,6H), 2.18-2.20(m,2H) 2.32-2.37 (m,3H), 3.02-3.08 (M, 2H?] 3.56-3.62(m,4H) 4.00-4.04 (d,1H) 4.12-4.15(d,1H) 4.27-4.40 (m,1H) 4.39-4.43(m,1H), 7.43-7.45 (d, 2H),7.52-7.54 (d,2H); LC/MS: m/z calculated 793.5, found 794.4 (M+1) + . 
     Step F: Intermediate 54 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)2,2-dimethylsuccinate 
     Added a solution of 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (55 g, 69.3 mmol) dissolved in methanol (825 mL), dimethyl amino acetaldehyde hydrogen sulfite sodium salt (66.8 g, 0.35 mol) and Et 3 N(38.7 g, 0.38 mol) into a reactor. The reaction was stirred for 2-3 hrs at 30-40° C. under nitrogen protection. NaBH 3 CN (8.8 g, 0.14 mol) was added and stirred at 30-40° C. under nitrogen protection for 13-15 hrs. Next, the reaction temperature was adjusted to 50-60° C. and stirred for 3-5 hrs. The reaction mixture was then concentrated to 275 mL and then DCM (550 mL) was added, followed by water (440 mL). The two phases were separated and the water phase was extracted with DCM (twice with 385 mLs each time). The organic phase was washed with water (twice with 358 mLs each time). Next, the organic phase was concentrated to give 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 43 g oil. Yield is 71%. 1HNMR (400 MHz, CDCl3) δ=7.27-7.29 (m, 4H),4.46-4.50 (m,1H), 4.08-4.13 (m,2H), 4.00-4.02 (m, 1H), 3.59-3.60 (m, 1H), 3.09 (m, 1H), 2.03-2.67 (m, 14H), 0.62-1.42 (m, 57H); LC/MS: m/z calculated 864.5, found 865.6 (M+1) + . 
     Step G: Compound 51 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     From the last step, dissolved the 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (0.8 g, 0.92 mmol) in 3.2 mL DCM, then charged 1.6 mL TFA into the solution. 
     The reaction mixture was then stirred at 15-25° C. for 1-3 hrs. Next, the reaction was charged with 4 mL DCM and 4 mL water, stirred well and then allowed to settle. The aqueous phase was then removed and the organic phase washed two times with water (4 mLs each). The aqueous phase was then extracted with DCM (1.6 mL). The combined organic phase was then washed with saturated sodium bicarbonate until the pH was between 7-8, followed by two more washes with water (4 mLs each). 
     Next, the organic phase was evaporated under vacuum to give 0.69 g crude product. The crude product was then dissolved in 4.8 mL isopropanol and heated to reflux to dissolve all material. Acetonitrile (19 mL) was added and brought to a gentle reflux and allowed to start cooling while seeded with 2 mg seed material at 45° C. The mixture was then stirred at 45° C. for 2 hours. Solids began form and they were cooled to 10-20° C. and stirred for 3 hours. The solution solids were filtered and then rinsed twice with 1 g acetonitrile each time. 
     The solids were then dried in a vacuum oven at 45 deg C. for 18 hours to give the desired product: 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (0.59 g, yield was 78%). 
     Example 499 
     Compound 51 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Procedures for steps A and D below are not included and are similar to those reported in previous examples and will be understood by one skilled in the art. 
     Step B: Intermediate 567 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     The starting material (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-9-hydroxy-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysene-3a-carbaldehyde (5 g, 11 mmol), 4-(tert-butoxy)-3,3-dimethyl-4-oxobutanoic acid (3.4 g, 16.5 mmol), THF (“tetrahydrofuran”—100 mL) and triethylamine (3.4 g, 33 mmol) were charged to a flask. 
     The mixture was then stirred for 20-30 mins at 0-5° C. Then, 4-methoxybenzoyl chloride (2.8 g, 16.5 mmol) was added through a dropping funnel while the reaction temperature was maintained at 0-5° C. The reaction was then stirred for an additional 1 hour, then DMAP (“dimethylaminopyridine”—0.2 g, 1.65 mmol) was added and the reaction allowed to stir at ambient temperature for approximately 24-30 hours until complete by HPLC. 
     Next, the reaction was charged with ethyl acetate (100 mL) and water (50 mL), stirred well and allowed to settle. The aqueous phase was removed and the organic phase washed with saturated sodium bicarbonate solution (2×50 mL) followed by water (50 mL). The reaction solution was evaporated under vacuum to approximately 5-10 mL to produce solids. An additional amount of heptane (50 mL) was added and the reaction heated back to reflux to nearly form solution and slowly cooled to ambient temperature and let stir for 15 minutes then cooled to 0° C. for approximately two hours and the solids were filtered out. Next, the solids were washed with heptane (2×50 mL) and dried at 50° C. under vacuum to give the desired compound as off-white solid 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate. (4.7 g, yield is 67%). 
     Step C: Intermediate 569 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     A reaction was charged with 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (6 g, 9.4 mmol), tert-butanol (60 mL) and toluene (18 mL) and temperature was adjusted to 20-25° C. The ligand (N1,N2-bis(1,7,7-trimethylbicyclo [2.2.1] heptan-2-yl)ethane-1,2-diamine (0.2 g, 0.57 mmol)) and Copper(I) acetate (0.06 g, 0.47 mmol) were added in a single portion and the reaction allowed to stir at 20-25° C. for 3-5 hrs. 
     The reaction was then cooled to a temperature of 7-13° C. Afterwards, the reaction was charged with nitromethane (3.9 g, 63 mmol) and allowed to stir at 7-13° C. for approximately 30 hrs. The reaction was charged with additional nitromethane (2.2 g, 35.7 mmol) and allowed to stir at 7-13° C. for approximately 40 hrs. The reaction was charged again with additional nitromethane (2.2 g, 35.7 mmol) and allowed to stir at 7-13° C. for approximately 20 hrs. 
     Next, the reaction was charged with MTBE (“methyl tertiary butyl ether”—90 mL) and a 15% ammonium chloride solution (36 mL), and allowed to stir for 30 min and then allowed to separate out into two phases. The organic phase was washed with water (30 mL) followed by brine (24 mL). The organic phase was then concentrated to 12 mL and MTBE (3 mL) was added. Next, n-heptane (60 mL) was slowly added and then stirred at 10-20° C. for 2 hrs. The solution was then filtered and washed with n-heptane, the cake was then dried under vacuum at 50-55° C. to give 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate as off-white solid 4.27 g. (65% yield) 
     Step E: Intermediate 571 
     1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate 
     4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 1-tert-butyl 2,2-dimethylsuccinate (15 g, 22.4 mmol) was placed in a round bottomed flask and then the following were also added: MgSO 4  powder (8.1 g, 56 mmol) and Et 3 N (3.6 g,35.8 mmol) and p-Chlorobenzaldehyde (5.05 g, 29.1 mmol) and added MeOH (300 mL). The reaction was then allowed to stir for 2 hrs. 
     The reaction mixture was then cooled to 0 degrees C. on ice-brine bath and treated with solid NaBH 4  (1.61 g, 42.56 mmol) under 10 degrees C. and allowed to stir at 20 degrees for approximately 45 minutes. HPLC after this time showed the reaction was complete. The reaction was then filtered through a Celite™ bed. The solids were rinsed with additional methanol (75 mL) and then dichloromethane (150 mL). The filtrate/rinse was treated with water (150 mL) and shaken in a separation funnel. The organic layers were then washed with water (150 mL). Next, the organic phase was evaporated to give 15 g of 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate as a foamy solid, 84% yield. The crude product was used for the next step directly. 
     Step F: Compound 46 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, 2,2,2-trifluoroacetic acid salt (1:1) 
     Dissolved 1-tert-butyl 4-((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl) 2,2-dimethylsuccinate (15 g, 18.9 mmol) in dichloromethane (150 mL) and treated with trifluoroacetic acid (30 mL) and allowed to stir at ambient temperature. The reaction was complete in approximately 10 hours. Next, the reaction washed with water (2×150 mL) and the organic phase was evaporated to a minimum volume and ethyl acetate (150 mL vol) was added and then evaporated again to a minimum volume. Evaporated again to a minimum volume and then dissolved in a total of 150 mL volume ethyl acetate. 
     Next, the resultant solution was heated to near reflux and then diluted with heptane (90 mL) and stirred while cooling to ambient temperature, at which point, solids were formed. The solution was then stirred for 30 minutes and filtered. The filtered solids were rinsed with heptane (30 mL) and dried to give 13 g 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, 2,2,2-trifluoroacetic acid salt (1:1), 81% yield. 1HNMR (400 MHz, DMSO-d6) δ=0.60-1.89(m, 46H) 2.16-2.19(d,1H) 2.32-2.68 (m, 4H) 3.02-3.11 (m,1H) 4.10-4.26 (m, 3H), 4.38-4.42(dd,1H) 5.93(s,1H) 7.51(s,4H), 8.80-8.98(d, 2H); LC-MS: m/z calculated (free base) 737.4, found 738.1 (M+1)+. 
     Step G: Compound 51 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     From Step F above, the 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid trifluoroacetic acid salt (9.0 g, 10.56 mmol) was placed in a round bottomed flask and added dimethylamino acetaldehyde hydrogen sulfite salt (5.05 g, 26.4 mmol) and CH 3 COONa (3.46 g, 42.23 mmol) and added MeOH (180 mL) and allowed to stir for 1 hour. 
     To the reaction mixture was added NaBH 3 CN (2.65 g, 42.23 mmol) and allowed to stir at 25 degrees C. for approximately 36 hrs. HPLC after this time showed reaction was complete. Next, the reaction was filtered through a Celite™ bed. The solids were then rinsed with additional methanol (45 mL) and then dichloromethane (90 mL). The filtrate/rinse was treated with water (45 mL) and shaken in separatory funnel. The organic layers were then washed with NaHCO 3  (4 times—45 mL each) and water (two times—45 mL each). 
     The organic phase was then evaporated to a minimum volume and i-PrOH (36 mL) was added and heated to 75 degrees C. for 2 hours. The resulting solution was then diluted with CH 3 CN (45 mL) at 75 degrees C. The solution was then cooled to 5 degrees C. over 4 hours. The solution was then stirred at 5 degrees C. for 2 hours and filtered. The filtrate was then rinsed with CH 3 CN (9 mL) and dried to give crude 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (5.6 g, 65% yield). 
     Re-Crystallization 
     From Step G above, the crude 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid (5.6 g) was placed in a round bottomed flask and added i-PrOH (36 mL) and heated to 75 degrees C. for 2 hours. Next, the reaction was diluted with CH 3 CN (45 mL) at 75 degrees C. The reaction was then cooled to 5 degrees C. over 4 hours. 
     The reaction was then stirred at 5 degrees C. for 2 hours and filtered. The filtrate was then rinsed with CH 3 CN (9 mL) and dried to give 4.8 g 4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid, 84% yield. 1HNMR (400 MHz, DMSO-d6) δ=0.80-0.29 (m,14H),0.97-1.17 (m, 21H),1.32-1.91 (m, 13), 2.08-2.23 (m,12H) 2.27-2.38 (m, 3H) 2.40-2.46 (m, 1H) 2.51-2.20 (m,1H) 2.66-2.70 (m,1H) 3.00-3.04 (m,1.0H) 3.71-3.78(m, 1H) 3.89-3.91(d, 1H) 4.37-4.41(dd, 1H), 7.31-7.37 (m,4H); LC-MS: m/z calculated 808.5, found 809.1 (M+1)+. 
     Synthesis of Intermediate 11S 
     
       
         
         
             
             
         
       
     
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-formyl-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (2.0 g, 4.03 mmol) was placed in a flask with n-BuOH (10 ml) and diamine catalyst derived from (R)-camphor (N1,N2-bis(1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl)ethane-1,2-diamine, 0.08 g, 0.242 mmol) diamine catalyst and Cu(OAc) 2  (0.037 g, 0.201 mmol) and stirred approximately 16 hours at ambient temperature. 
     The reaction was then cooled to approximately 0° C. and treated with nitromethane (1.23 g, 20.1 mmol) and Hunig&#39;s base ((“N,N-diisopropylethylamine”—0.053 g, 0.40 mmol) and allowed to held at 5° C. for 24 hours. Additional BuOH (25 ml) and Hunig&#39;s base (“N,N-diisopropylethylamine”) were added and the reaction held at −10° C. for 24 hours and then at ambient temperature for 24 hours. Solids present in the reaction were filtered to provide the title compound as an off white solid (1.1 g, 49% as ˜95:5 mixture of diastereomers).  1 H NMR (500 MHz, CDCl3) δ=4.89 (bd, 1H), 4.57 (d, 1H), 4.49 (dd, 1H), 4.34 (dd, 1H), 3.21 (m, 1H) 2.94 (dd, 1H) 2.38 (m, 2H) 2.08 to 0.8 (m, 54H) *Note: Integration of the 2.4 to 0.8 region of the spectrum is higher due to the presence of impurities in the sample. 
     Synthesis of Intermediate 12S 
     
       
         
         
             
             
         
       
     
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a flask containing (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-1-hydroxy-2-nitroethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (˜95% single diastereomer from the previous Intermediate 11S stage, 0.5 g, 0.89 mmol) in methanol and cooled to −5° C. was added NiCl 2  (0.232 g, 1.79 mmol). The contents were stirred and treated with NaBH 4  (0.475 g, 12.5 mmol), which was added slowly in portions over a 30 minute period while maintaining at approximately −5° C. 
     An extremely exothermic reaction resulted in some material loss. The reaction was warmed to ambient temperature slowly over a 30 minute period and allowed to stir for approximately 2 hours. The reaction was then quenched with NH 4 Cl soln and diluted with methylene chloride. The liquid layers were separated and the organic layer was washed several times with NH 4 Cl soln. Next, the organic layer was evaporated to dryness and the residue was chromatographed on silica gel column and eluted with 10% methanol-dichloromethane. The product containing fractions were evaporated to give title compound as grey solid (250 mg, 53% yield). Sample was carried on to next stage without further characterization. 
     Synthesis of Intermediate 13S 
     
       
         
         
             
             
         
       
     
     (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-((4-chlorobenzyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate 
     To a flask containing (3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((S)-2-amino-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl acetate (200 mg, 379 mmol) and methanol (15 ml) was added triethylamine (15 mg, 0.152 mmol), magnesium sulfate (68 mg, 0.168 mmol) and 4-chlorobenzaldehyde (64 mg, 0.455 mmol). The contents were allowed to stir at ambient temperature for approximately 2 hours. To the resulting mixture was added sodium borohydride (17 mg, 455 mmol) in a single portion and the reaction was allowed to stir at ambient temperature for two and a half days. The reaction was then charged with an additional 20% excess of the p-Cl-benzaldehyde and sodium borohydride and allowed to stir an additional 2 hours. Finally, the reaction was quenched with water, allowed to stir 15 minutes and solids filtered to provide title compound contaminated with impurities. LC/MS: m/z calculated 651.4, found 652 (M+1) + . 
     Synthesis of (S) Camphor Derived Chiral Diamine Ligand 568 
     
       
         
         
             
             
         
       
     
     Step A: N,N′-bis(isobornyl)ethylenediimine (573) 
     Titanium (IV) isopropoxide (235.4 g, 830 mmol, 1.04 eq) was added to a flask containing (1S)-(−)-camphor (121.43 g, 798 mmol, 1 eq) at ambient temperature. The reaction was then heated to ˜50° C. Next, ethylenediamine (31.2 g, 518 mmol, 0.65 eq) was charged to the reaction. The temperature was then kept above 45° C. during the addition. The reaction was then heated to ˜91° C. for 17 hours. Next, the reaction was cooled to 20-25° C. and heptane (1.2 L) was added. Water (29.9 g, 1659 mmol, 2.08 eq) was added over at least 15 minutes. The slurry was then stirred for 20 minutes at ambient temperature, cooled to ˜0° C., and stirred for 30 minutes at ˜0° C. The slurry was then filtered and the solids washed with heptane (607 mL). The diimine solution was stored ˜5° C. overnight. The solution was then warmed to ambient temperature and filtered to remove additional salts. Next, the solution was partially concentrated and filtered through Celite™. Finally, the solution was concentrated to ˜608 mL and used as is in the next reaction. 
     Step B: N,N′-bis(isobornyl)ethylenediamine ligand (568). 
     To a 1 L Jacketed Lab Reactor (JLR) was added the above diimine solution followed by 5% Pt/C (Johnson-Matthey, B501018-5, 6.6 g). The reaction was hydrogenated for ˜15 hours at 4 par at ambient temperature. The reaction was filtered and washed with heptane (300 mL). The solution was concentrated to provide a white solid (115.07 g). This two step procedure was repeated. Both batches were combined. Attempts to crystallize the material from i-PrOH and water failed. The product was extracted with heptane. The heptane layer was then washed with water, brine, dried over sodium sulfate, filtered and concentrated on rotovapor and then high vacuum. The product 568 (222.18 g) was obtained as a white solid and used as is in the asymmetric Henry reaction during the camphor ligand addition step.  1 H NMR (500 MHz, CDCl 3 ) δ 2.69-2.61 (m, 1H), 2.53-2.47 (m, 2H), 1.71-1.63 (m, 2H), 1.6-1.43 (m, 3H), 1.1-1.01 (m, 2H), 1.01-0.98 (m, 3H), 0.89-0.83 (m, 3H), 0.81-0.78 (m, 3H) 
     Synthesis of (R) Camphor Derived Chiral Diamine Ligand 572 
     
       
         
         
             
             
         
       
     
     This material was prepared with modifications to methods shown in Tetrahedron:  Asymmetry  14 (2003) 1451-1454. 
     Step A: N,N′-bis(isobornyl)ethylenediimine (574). 
     Titanium isopropoxide (100 mL, 341 mmol, 1.04 eq) was charged to a roundbottom flask containing (1R)-(+)-camphor (50 g, 328 mmol) at 20-25° C. The reaction mixture was then heated to 50° C., at which time ethylenediamine (14.3 mL, 213 mmol, 0.65 eq) was charged to the reaction. Note: The ethylenediamine addition was exothermic, but it is important to conduct the charge above 45° C. to prevent solidification of the reaction mixture as titanium/ethylenediamine complexes are formed. The solution was then heated to reflux (˜90-92° C.) for 20-24 hours. At that time, the reaction is cooled to 20-25° C. and diluted with heptane (500 mL), followed by slow addition of H 2 O (12.3 mL, 2.08 eq) over ˜15 minutes to initiate the formation (and subsequent precipitation) of TiO 2  from the reaction. The slurry was stirred for 20 minutes at 20-25° C., cooled to 0° C., and stirred for 20 minutes at 0° C. Finally, the precipitate was filtered and washed with heptane (250 mL) to obtain a solution of diimine (573) in heptane, which was concentrated to 250 mL of heptane (˜4.6 vol) prior to use in the subsequent hydrogenation stage. 
     Step B: N,N′-bis(isobornyl)ethylenediamine ligand (572) 
     Johnson-Matthey Yellow Kit #28 [5% Pt/C, B501018-5] catalyst (2.7 g, 5 wt % wrt 6) was charged to a Parr vessel, followed by a solution of diimine (6) in heptane (˜54 g in 250 mL heptane). The reaction was hydrogenated for 15 h at 100 psi H 2 , 20° C., at which time the only product was observed by LCMS. The mixture was then filtered through a celite pad and washed with heptane (130 mL). The filtrate solvent was swapped from heptanes to isopropanol (270 mL, 5 vol), after which water (86 mL, 1.5 vol) was charged and the reaction was heated to reflux to dissolve all material. Slow cooling of the solution to 20° C. resulted in crystallization of the product. Additional water (200 mL, 3.7 vol) was charged over 15 minutes and the reaction was held for an additional 20 minutes. The slurry was then cooled to 0° C. and held for 30 minutes, at which time the product was filtered and washed with a 0° C. mixture of 1:1 water/isopropanol (130 mL) to yield 46.05 g (572) (84.3%) after drying overnight. 
     The above intermediates 11S, 12S, and 13S serve to demonstrate application of the asymmetric Henry reaction in the camphor ligand step to produce the S alcohol on a useful intermediate in consistent yield and selectivity with the earlier examples that provide the R diastereomeric alcohol. It will be apparent to one skilled in the art that this methodology can be employed in combination with the appropriate triterpene derived scaffolds to give either the R or S diastereomer upon choosing the appropriate camphor derived diamine chiral ligand under the conditions outlined herein. Furthermore, it is shown that the above nitroalcohol intermediates, whether a mixture of diastereomeric alcohols or in optically enriched form, are useful to provide compounds of Formula I or Formula II. 
     Example 500 
     Compound 575 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((5-chloropyrimidin-2-yl)methyl)(2-(2-oxopyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 850.5, found 851.5 (M+1) +   
     Example 501 
     Compound 576 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((5-chloropyrimidin-2-yl)methyl)(2-(dimethylamino)-2-oxoethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 824.5, found 825.5 (M+1) +   
     Example 502 
     Compound 577 
     (R)-4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2-methyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 724.5, found 725.5 (M+1) +   
     Example 503 
     Compound 578 
     (R)-4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2-methyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     Example 504 
     Compound 579 
     4-(((3aR,5aR,5bR,7aR,8S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(pyridazin-3-ylmethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 776.5, found 777.6 (M+1) +   
     Example 505 
     Compound 580 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclobutylmethyl)(2-(2-oxopyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 792.6, found 793.6 (M+1) +   
     Example 506 
     Compound 581 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((S)-1-(5-chloropyrimidin-2-yl)ethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 824.5, found 825.5 (M+1) +   
     Example 507 
     Compound 582 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-(((R)-1-(5-chloropyrimidin-2-yl)ethyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 824.5, found 825.5 (M+1) +   
     Example 508 
     Compound 583 
     (R)-4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((2-(dimethylamino)ethyl)(isopropyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2-methyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 712.5, found 713.7 (M+1) +   
     Example 509 
     Compound 584 
     4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((cyclopropylmethyl)(2-(2-oxopyrrolidin-1-yl)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2,2-dimethyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 778.6, found 779.6 (M+1) +   
     Example 510 
     Compound 585 
     (S)-4-(((3aR,5aR,5bR,7aR,9S,11aR,11bR,13aS)-3a-((R)-2-((4-chlorobenzyl)(2-(dimethylamino)ethyl)amino)-1-hydroxyethyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2-oxo-3,3a,4,5,5a,5b,6,7,7a,8,9,10,11,11a,11b,12,13,13a-octadecahydro-2H-cyclopenta[a]chrysen-9-yl)oxy)-2-methyl-4-oxobutanoic acid 
     
       
         
         
             
             
         
       
     
     LC/MS: m/z calculated 794.5, found 795.5 (M+1) +   
     After the above description of synthesis procedures for the compounds described herein, there is provided in accordance with other embodiments of the present invention, a process for preparing a compound of Formula (I) having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) saponifying a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of a first metal catalyst, in order to provide a compound having the structure: 
     
       
         
         
             
             
         
       
         
         
           
             (2) reacting the compound product of Step (1), with a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
     
     , in the presence of an acid chloride and a tertiary amine in order to provide a compound having the structure: 
     
       
         
         
             
             
         
       
         
         
           
             (3) reacting nitromethane with the compound product of Step (2) in the presence of a chiral ligand, an optional base, and a second metal catalyst in order to provide a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (4) reducing the compound product of Step (3), in the presence of a third metal catalyst and hydrogen gas in order to provide a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (5) reacting p-chlorobenzaldehyde with the compound product of Step (4), in the presence of a metal hydride, to provide a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (6) acidifying the compound product of Step (5), in the presence of an acid in order to provide the compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (7) reacting the compound product of Step (6) with a compound having the structure according to Formula III: 
           
         
       
    
     
       
         
         
             
             
         
       
     
     wherein A 1  is either optionally absent or is selected from the group consisting —H, methyl, and ethyl; and A 2  is selected from the group consisting of —H, methoxy, ethoxy, hydroxyl, and —SO 3   − Na + ; while in the presence of a metal hydride in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     As will be known to one of skill in the art after reading the synthetic procedures described here and the process steps described above and below, several suitable chemical components can be used to carry out the process steps. Such suitable chemical components can be used interchangeably with any process step description described herein according to knowledge in the art. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein one or more of Steps (1)-(7) are conducted in the presence of a solvent. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein one or more of Steps (1)-(7) are conducted in the presence of an organic solvent. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein one or more of Steps (1)-(7) are conducted in the presence of a solvent that is selected from the group consisting of water, dichloromethyl, methanol, tetrahydrofuran, tetrahydrofuran acetate, ethanol, ethyl acetate, heptane, isopropanol, tert-butanol, toluene, acetonitrile, and tert-butyl methyl ether. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the first metal catalyst of Step (1) is a metal halide. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the first metal catalyst of Step (1) is zirconium tetrachloride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the acid chloride of Step (2) is selected from the group consisting of benzoyl chloride and methoxybenzoyl chloride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the acid chloride of Step (2) is methoxybenzoyl chloride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (2) is a tertiary amine coupling agent. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (2) is selected from the group consisting of triethylamine, N,N-diisopropylethylamine, and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (2) is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (2) is triethylamine. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the second metal catalyst of Step (3) is a compound comprising a metal selected from the group consisting of Zn, Co, Cu, Mg, and Cr. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the second metal catalyst of Step (3) is a compound comprising a metal selected from the group consisting of Cu(I) and Cu(II). 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the second metal catalyst of Step (3) is a compound comprising Cu(I). 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the second metal catalyst of Step (3) is selected from the group consisting of Copper(I) acetate and Cu(II) acetate. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the second metal catalyst of Step (3) is Copper(I) acetate. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the second metal catalyst of Step (3) is Copper(II) acetate. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the optional base of Step (3) is selected from the group consisting of alkali metal hydroxides, alkoxides, carbonates, fluoride anions, and nonionic organic amine bases. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the optional base of Step (3) is  i Pr 2 Net. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the chiral ligand of Step (3) is a derivative of S-Camphor. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the chiral ligand of Step (3) is a compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the third metal catalyst of Step (4) is selected from the group consisting of Raney Nickel, nickel, nickel chloride, aluminum, palladium, copper, zinc, chromium, iridium, rhodium, platinum, and combinations thereof. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the third metal catalyst of Step (4) is Raney Nickel. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the hydrogen of Step (4) is hydrogen gas. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is a borohydride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is selected from the group consisting of NaBH 4  and NaBH(OAc) 3 . 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is NaBH 4 . 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is NaBH(OAc) 3 . 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the acid of Step (6) is selected from the group consisting of trifluoroacetic acid, HCl, and trichloroacetic acid, 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the acid of Step (6) is trifluoroacetic acid. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the A 2  of Step (7) is —SO 3 Na. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the A 2  of Step (7) is —H. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the A 1  of Step (7) is absent. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the A 1  of Step (7) is —H. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the compound of Formula III of Step (7) has the following structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the compound of Formula III of Step (7) has the following structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the compound of Formula III of Step (7) has the following structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the compound of Formula III of Step (7) has the following structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the compound of Formula III of Step (7) has the following structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (7) is a borohydride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (7) is NaBH 3 CN. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I) having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) saponifying a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of a first metal catalyst, in order to provide a compound having the structure: 
     
       
         
         
             
             
         
       
         
         
           
             (2) reacting the compound product of Step (1), with a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
     
     in the presence of an acid chloride and a tertiary amine in order to provide a compound having the structure: 
     
       
         
         
             
             
         
       
         
         
           
             (3) reacting nitromethane with the compound product of Step (2) in the presence of a chiral ligand and a base and a second metal catalyst in order to provide a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (4) reducing the compound product of Step (3), in the presence of a third metal catalyst and hydrogen gas in order to provide a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (5) reacting p-chlorobenzaldehyde with the compound product of Step (4), in the presence of a metal hydride and a tertiary amine, to provide a compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
         
         
           
             (6) reacting the compound product of Step (5) with a compound having the structure according to Formula III: 
           
         
       
    
     
       
         
         
             
             
         
       
     
     wherein A 1  is either optionally absent or is selected from the group consisting —H, methyl, and ethyl; and A 2  is selected from the group consisting of —H, methoxy, ethoxy, hydroxyl, and —SO 3   − Na + ; while in the presence of a metal hydride in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
         
         
           
             (7) acidifying the compound product of Step (6), in the presence of an acid in order to provide the compound having the structure: 
           
         
       
    
     
       
         
         
             
             
         
       
     
     With regards to the synthesis procedures herein, and in accordance with other embodiments of the present invention, there is also provided a process for preparing a compound of Formula (I), wherein one or more of Steps (1)-(7) are conducted in the presence of a solvent. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein one or more of Steps (1)-(7) are conducted in the presence of an organic solvent. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein one or more of Steps (1)-(7) are conducted in the presence of a solvent that is selected from the group consisting of water, dichloromethyl, methanol, tetrahydrofuran, tetrahydrofuran acetate, ethanol, ethyl acetate, heptane, isopropanol, tert-butanol, toluene, acetonitrile, and tert-butyl methyl ether. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the base of Step (3) is selected from the group consisting of alkali metal hydroxides, alkoxides, carbonates, fluoride anions, and nonionic organic amine bases. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the base of Step (3) is  i Pr 2 Net. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (5) is selected from the group consisting of triethylamine and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (5) is triethylamine. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is a borohydride. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is selected from the group consisting of NaBH 4  and NaBH(OAc) 3 . 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the metal hydride of Step (5) is NaBH 4 . 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), wherein the tertiary amine of Step (6) is triethylamine. 
     In accordance with other embodiments of the present invention, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) reacting a compound having the structure:       

     
       
         
         
             
             
         
       
         
         
           
              with a compound having the structure according to Formula III: 
           
         
       
    
     
       
         
         
             
             
         
       
     
     wherein A 1  is either optionally absent or is selected from the group consisting —H, methyl, and ethyl; and A 2  is selected from the group consisting of —H, methoxy, ethoxy, hydroxyl, and —SO 3   − Na + ; while in the presence of a metal hydride in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) acidifying a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of an acid in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) reacting p-chlorobenzaldehyde with a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of a metal hydride to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) reducing a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of a metal catalyst and hydrogen gas in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) reacting nitromethane with a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of a chiral ligand and a metal catalyst in order to provide a compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) reacting a compound having the structure:       

     
       
         
         
             
             
         
       
     
     with a compound having the structure: 
     
       
         
         
             
             
         
       
     
     in the presence of an acid chloride and a tertiary amine in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps of:
         (1) saponifying a compound having the structure:       

     
       
         
         
             
             
         
       
     
     in the presence of a metal catalyst, in order to provide the compound having the structure: 
     
       
         
         
             
             
         
       
     
     In accordance with other embodiments of the present invention and the suitable process step components recited above, there is provided a process for preparing a compound of Formula (I), having the structure: 
     
       
         
         
             
             
         
       
     
     comprising the steps according to the following synthesis scheme. As will be known to one of skill in the art, the above reagents recited in still other synthesis schemes may be substituted within the synthesis scheme below as described above. 
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
     Administration and Formulation 
     In another embodiment, there is provided a pharmaceutical composition comprising a pharmaceutically acceptable diluent and a therapeutically effective amount of a compound of Formula I or Formula II or a pharmaceutically acceptable salt thereof. 
     The compounds of the present invention can be supplied in the form of a pharmaceutically acceptable salt. The terms “pharmaceutically acceptable salt” refer to salts prepared from pharmaceutically acceptable inorganic and organic acids and bases. Accordingly, the word “or” in the context of “a compound or a pharmaceutically acceptable salt thereof” is understood to refer to either a compound or a pharmaceutically acceptable salt thereof (alternative), or a compound and a pharmaceutically acceptable salt thereof (in combination). 
     As used herein, the term “pharmaceutically acceptable” refers to those compounds, materials, compositions, and dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, or other problem or complication. The skilled artisan will appreciate that pharmaceutically acceptable salts of compounds according to Formula I or Formula II may be prepared. These pharmaceutically acceptable salts may be prepared in situ during the final isolation and purification of the compound, or by separately reacting the purified compound in its free acid or free base form with a suitable base or acid, respectively. 
     Illustrative pharmaceutically acceptable acid salts of the compounds of the present invention can be prepared from the following acids, including, without limitation formic, acetic, propionic, benzoic, succinic, glycolic, gluconic, lactic, maleic, malic, tartaric, citric, nitric, ascorbic, glucuronic, maleic, fumaric, pyruvic, aspartic, glutamic, benzoic, hydrochloric, hydrobromic, hydroiodic, isocitric, trifluoroacetic, pamoic, propionic, anthranilic, mesylic, oxalacetic, oleic, stearic, salicylic, p-hydroxybenzoic, nicotinic, phenylacetic, mandelic, embonic (pamoic), methanesulfonic, phosphoric, phosphonic, ethanesulfonic, benzenesulfonic, pantothenic, toluenesulfonic, 2-hydroxyethanesulfonic, sulfanilic, sulfuric, salicylic, cyclohexylaminosulfonic, algenic, β-hydroxybutyric, galactaric and galacturonic acids. Preferred pharmaceutically acceptable salts include the salts of hydrochloric acid and trifluoroacetic acid. 
     Illustrative pharmaceutically acceptable inorganic base salts of the compounds of the present invention include metallic ions. More preferred metallic ions include, but are not limited to, appropriate alkali metal salts, alkaline earth metal salts and other physiological acceptable metal ions. Salts derived from inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic salts, manganous, potassium, sodium, zinc, and the like and in their usual valences. Exemplary base salts include aluminum, calcium, lithium, magnesium, potassium, sodium and zinc. Other exemplary base salts include the ammonium, calcium, magnesium, potassium, and sodium salts. Still other exemplary base salts include, for example, hydroxides, carbonates, hydrides, and alkoxides including NaOH, KOH, Na 2 CO 3 , K 2 CO 3 , NaH, and potassium-t-butoxide. 
     Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, including in part, trimethylamine, diethylamine, N,N′-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, meglumine (N-methylglucamine) and procaine; substituted amines including naturally occurring substituted amines; cyclic amines; quaternary ammonium cations; and basic ion exchange resins, such as arginine, betaine, caffeine, choline, N,N-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine and the like. 
     All of the above salts can be prepared by those skilled in the art by conventional means from the corresponding compound of the present invention. For example, the pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemical methods. Generally, such salts can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. The salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent. The degree of ionisation in the salt may vary from completely ionised to almost non-ionised. Lists of suitable salts are found in  Remington&#39;s Pharmaceutical Sciences,  17th ed., Mack Publishing Company, Easton, Pa., 1985, p. 1418, the disclosure of which is hereby incorporated by reference only with regards to the lists of suitable salts. 
     The compounds of the invention may exist in both unsolvated and solvated forms. The term ‘solvate’ is used herein to describe a molecular complex comprising the compound of the invention and one or more pharmaceutically acceptable solvent molecules, for example, ethanol. The term ‘hydrate’ is employed when said solvent is water. Pharmaceutically acceptable solvates include hydrates and other solvates wherein the solvent of crystallization may be isotopically substituted, e.g. D 2 O, d 6 -acetone, d6-DMSO. 
     Compounds of Formula I or Formula II containing one or more asymmetric carbon atoms can exist as two or more stereoisomers. Where a compound of Formula I or Formula II contains an alkenyl or alkenylene group or a cycloalkyl group, geometric cis/trans (or Z/E) isomers are possible. Where the compound contains, for example, a keto or oxime group or an aromatic moiety, tautomeric isomerism (‘tautomerism’) can occur. It follows that a single compound may exhibit more than one type of isomerism. 
     Included within the scope of the claimed compounds present invention are all stereoisomers, geometric isomers and tautomeric forms of the compounds of Formula I or Formula II, including compounds exhibiting more than one type of isomerism, and mixtures of one or more thereof. Also included are acid addition or base salts wherein the counterion is optically active, for example, D-lactate or L-lysine, or racemic, for example, DL-tartrate or DL-arginine. 
     Cis/trans isomers may be separated by conventional techniques well known to those skilled in the art, for example, chromatography and fractional crystallisation. 
     Conventional techniques for the preparation/isolation of individual enantiomers include chiral synthesis from a suitable optically pure precursor or resolution of the racemate (or the racemate of a salt or derivative) using, for example, chiral high pressure liquid chromatography (HPLC). 
     Alternatively, the racemate (or a racemic precursor) may be reacted with a suitable optically active compound, for example, an alcohol, or, in the case where the compound of Formula I or Formula II contains an acidic or basic moiety, an acid or base such as tartaric acid or 1-phenylethylamine. The resulting diastereomeric mixture may be separated by chromatography and/or fractional crystallization and one or both of the diastereoisomers converted to the corresponding pure enantiomer(s) by means well known to a skilled person. 
     Chiral compounds of the invention (and chiral precursors thereof) may be obtained in enantiomerically-enriched form using chromatography, typically HPLC, on a resin with an asymmetric stationary phase and with a mobile phase consisting of a hydrocarbon, typically heptane or hexane, containing from 0 to 50% isopropanol, typically from 2 to 20%, and from 0 to 5% of an alkylamine, typically 0.1% diethylamine. Concentration of the eluate affords the enriched mixture. 
     Mixtures of stereoisomers may be separated by conventional techniques known to those skilled in the art. [see, for example, “Stereochemistry of Organic Compounds” by E L Eliel (Wiley, N.Y., 1994).] 
     The present invention includes all pharmaceutically acceptable isotopically-labelled compounds of Formula I or Formula II wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature. 
     Examples of isotopes suitable for inclusion in the compounds of the invention include isotopes of hydrogen, such as  2 H and  3 H, carbon, such as  11 C,  13 C and  14 C, chlorine, such as  36 Cl, fluorine, such as  18 F, iodine, such as  123 I and  125 I, nitrogen, such as  13 N and  15 N, oxygen, such as  15 O,  17 O and  18 O, phosphorus, such as  32 P, and sulphur, such as  35 S. 
     Certain isotopically-labelled compounds of Formula I or Formula II, for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i.e.  3 H, and carbon-14, i.e.  14 C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection. 
     Substitution with heavier isotopes such as deuterium, i.e.  2 H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances. 
     Isotopically-labelled compounds of Formula I or Formula II can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Preparations using an appropriate isotopically-labelled reagents in place of the non-labelled reagent previously employed. 
     The compounds of the present invention may be administered as prodrugs. Thus, certain derivatives of compounds of Formula I or Formula II, which may have little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of Formula I or Formula II as ‘prodrugs’. 
     Administration of the chemical entities described herein can be via any of the accepted modes of administration for agents that serve similar utilities including, but not limited to, orally, sublingually, subcutaneously, intravenously, intranasally, topically, transdermally, intraperitoneally, intramuscularly, intrapulmonarilly, vaginally, rectally, or intraocularly. In some embodiments, oral or parenteral administration is used. 
     Pharmaceutical compositions or formulations include solid, semi-solid, liquid and aerosol dosage forms, such as, e.g., tablets, capsules, powders, liquids, suspensions, suppositories, aerosols or the like. The chemical entities can also be administered in sustained or controlled release dosage forms, including depot injections, osmotic pumps, pills, transdermal (including electrotransport) patches, and the like, for prolonged and/or timed, pulsed administration at a predetermined rate. In certain embodiments, the compositions are provided in unit dosage forms suitable for single administration of a precise dose. 
     The chemical entities described herein can be administered either alone or more typically in combination with a conventional pharmaceutical carrier, excipient or the like (e.g., mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, sodium crosscarmellose, glucose, gelatin, sucrose, magnesium carbonate, and the like). If desired, the pharmaceutical composition can also contain minor amounts of nontoxic auxiliary substances such as wetting agents, emulsifying agents, solubilizing agents, pH buffering agents and the like (e.g., sodium acetate, sodium citrate, cyclodextrine derivatives, sorbitan monolaurate, triethanolamine acetate, triethanolamine oleate, and the like). Generally, depending on the intended mode of administration, the pharmaceutical composition will contain about 0.005% to 95%; in certain embodiments, about 0.5% to 50% by weight of a chemical entity. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see  Remington&#39;s Pharmaceutical Sciences,  Mack Publishing Company, Easton, Pa. 
     In certain embodiments, the compositions will take the form of a pill or tablet and thus the composition will contain, along with the active ingredient, a diluent such as lactose, sucrose, dicalcium phosphate, or the like; a lubricant such as magnesium stearate or the like; and a binder such as starch, gum acacia, polyvinylpyrrolidine, gelatin, cellulose, cellulose derivatives or the like. In another solid dosage form, a powder, marume, solution or suspension (e.g., in propylene carbonate, vegetable oils or triglycerides) is encapsulated in a gelatin capsule. 
     Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, etc. at least one chemical entity and optional pharmaceutical adjuvants in a carrier (e.g., water, saline, aqueous dextrose, glycerol, glycols, ethanol or the like) to form a solution or suspension. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions, as emulsions, or in solid forms suitable for dissolution or suspension in liquid prior to injection. The percentage of chemical entities contained in such parenteral compositions is highly dependent on the specific nature thereof, as well as the activity of the chemical entities and the needs of the subject. However, percentages of active ingredient of 0.01% to 10% in solution are employable, and will be higher if the composition is a solid which will be subsequently diluted to the above percentages. In certain embodiments, the composition will comprise from about 0.2 to 2% of the active agent in solution. 
     Pharmaceutical compositions of the chemical entities described herein may also be administered to the respiratory tract as an aerosol or solution for a nebulizer, or as a microfine powder for insufflation, alone or in combination with an inert carrier such as lactose. In such a case, the particles of the pharmaceutical composition have diameters of less than 50 microns, in certain embodiments, less than 10 microns. 
     In general, the chemical entities provided will be administered in a therapeutically effective amount by any of the accepted modes of administration for agents that serve similar utilities. The actual amount of the chemical entity, i.e., the active ingredient, will depend upon numerous factors such as the severity of the disease to be treated, the age and relative health of the subject, the potency of the chemical entity used the route and form of administration, and other factors. The drug can be administered more than once a day, such as once or twice a day. 
     Therapeutically effective amounts of the chemical entities described herein may range from approximately 0.01 to 200 mg per kilogram body weight of the recipient per day; such as about 0.01-100 mg/kg/day, for example, from about 0.1 to 50 mg/kg/day. Thus, for administration to a 70 kg person, the dosage range may be about 7-3500 mg per day. 
     In general, the chemical entities will be administered as pharmaceutical compositions by any one of the following routes: oral, systemic (e.g., transdermal, intranasal or by suppository), or parenteral (e.g., intramuscular, intravenous or subcutaneous) administration. In certain embodiments, oral administration with a convenient daily dosage regimen that can be adjusted according to the degree of affliction may be used. Compositions can take the form of tablets, pills, capsules, semisolids, powders, sustained release formulations, solutions, suspensions, elixirs, aerosols, or any other appropriate compositions. Another manner for administering the provided chemical entities is inhalation. 
     The choice of formulation depends on various factors such as the mode of drug administration and bioavailability of the drug substance. For delivery via inhalation the chemical entity can be formulated as liquid solution, suspensions, aerosol propellants or dry powder and loaded into a suitable dispenser for administration. There are several types of pharmaceutical inhalation devices-nebulizer inhalers, metered dose inhalers (MDI) and dry powder inhalers (DPI). Nebulizer devices produce a stream of high velocity air that causes the therapeutic agents (which are formulated in a liquid form) to spray as a mist that is carried into the patient&#39;s respiratory tract. MDIs typically are formulation packaged with a compressed gas. Upon actuation, the device discharges a measured amount of therapeutic agent by compressed gas, thus affording a reliable method of administering a set amount of agent. DPI dispenses therapeutic agents in the form of a free flowing powder that can be dispersed in the patient&#39;s inspiratory air-stream during breathing by the device. In order to achieve a free flowing powder, the therapeutic agent is formulated with an excipient such as lactose. A measured amount of the therapeutic agent is stored in a capsule form and is dispensed with each actuation. 
     Recently, pharmaceutical compositions have been developed for drugs that show poor bioavailability based upon the principle that bioavailability can be increased by increasing the surface area i.e., decreasing particle size. For example, U.S. Pat. No. 4,107,288 describes a pharmaceutical formulation having particles in the size range from 10 to 1,000 nm in which the active material is supported on a cross-linked matrix of macromolecules. U.S. Pat. No. 5,145,684 describes the production of a pharmaceutical formulation in which the drug substance is pulverized to nanoparticles (average particle size of 400 nm) in the presence of a surface modifier and then dispersed in a liquid medium to give a pharmaceutical formulation that exhibits remarkably high bioavailability. 
     The compositions are comprised of, in general, at least one chemical entity described herein in combination with at least one pharmaceutically acceptable excipient. Acceptable excipients are non-toxic, aid administration, and do not adversely affect the therapeutic benefit of the at least one chemical entity described herein. Such excipient may be any solid, liquid, semi-solid or, in the case of an aerosol composition, gaseous excipient that is generally available to one of skill in the art. 
     Solid pharmaceutical excipients include starch, cellulose, talc, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, magnesium stearate, sodium stearate, glycerol monostearate, sodium chloride, dried skim milk and the like. Liquid and semisolid excipients may be selected from glycerol, propylene glycol, water, ethanol and various oils, including those of petroleum, animal, vegetable or synthetic origin, e.g., peanut oil, soybean oil, mineral oil, sesame oil, etc. Liquid carriers, for injectable solutions, include water, saline, aqueous dextrose, and glycols. 
     Compressed gases may be used to disperse a chemical entity described herein in aerosol form. Inert gases suitable for this purpose are nitrogen, carbon dioxide, etc. Other suitable pharmaceutical excipients and their formulations are described in Remington&#39;s Pharmaceutical Sciences, edited by E. W. Martin (Mack Publishing Company, 18th ed., 1990). 
     The amount of the chemical entity in a composition can vary within the full range employed by those skilled in the art. Typically, the composition will contain, on a weight percent (wt %) basis, from about 0.01-99.99 wt % of at least one chemical entity described herein based on the total composition, with the balance being one or more suitable pharmaceutical excipients. In certain embodiments, the at least one chemical entity described herein is present at a level of about 1-80 wt %. 
     Example 84 
     MT4 Cell Antiviral Assay 
     Experimental Procedure: 
     Antiviral HIV activity and compound-induced cytotoxicity were measured in parallel by means of a propidium iodide based procedure in the human T-cell lymphotropic virus transformed cell line MT4. Aliquots of the test compounds were serially diluted in medium (RPMI 1640, 10% fetal calf serum (FCS), and gentamycin) in 96-well plates (Costar 3598) using a Cetus Pro/Pette. Exponentially growing MT4 cells were harvested and centrifuged at 1000 rpm for 10 min in a Jouan centrifuge (model CR 4 12). Cell pellets were resuspended in fresh medium (RPMI 1640, 20% FCS, 20% IL-2, and gentamycin) to a density of 5×105 cells/ml. Cell aliquots were infected by the addition of HIV-1 (strain IIIB) diluted to give a viral multiplicity of infection of 100×TCID50. A similar cell aliquot was diluted with medium to provide a mock-infected control. Cell infection was allowed to proceed for 1 hr at 37° C. in a tissue culture incubator with humidified 5% CO 2  atmosphere. After the 1 hr incubation the virus/cell suspensions were diluted 6-fold with fresh medium, and 125 μl of the cell suspension was added to each well of the plate containing pre-diluted compound. Plates were then placed in a tissue culture incubator with humidified 5% CO 2  for 5 days. At the end of the incubation period, cell number and hence HIV-induced cytopathy was estimated by either (A) propidium iodide staining, or by an (B) MTS tetrazolium staining method. 
     For propidium iodide readout, 27 μl of 5% Nonidet-40 was added to each well of the incubation plate. After thorough mixing with a Costar multitip pipetter, 60 μl of the mixture was transferred to filter-bottomed 96-well plates. The plates were analyzed in an automated assay instrument (Screen Machine, Idem(Laboratories). The control and standard used was 3′-azido-3′-deoxythymidine tested over a concentration range of 0.01 to 1 μM in every assay. The expected range of IC 50  values for 3′-azido-3′-deoxythymidine is 0.04 to 0.12 μM. The assay makes use of a propidium iodide dye to estimate the DNA content of each well. 
     For MTS readout, 20 μl CellTiter 96 AQ One Solution reagent (Promega #G3582) was added to each well. At 75 minutes following the addition of MTS reagent, absorbance was read at 492 nM using a Tecan Sunrise 96-well plate reader. 
     Analysis: 
     The antiviral effect of a test compound is reported as an EC 50 , i.e. the inhibitory concentration that would produce a 50% decrease in the HIV-induced cytopathic effect. This effect is measured by the amount of test compound required to restore 50% of the cell growth of HIV-infected MT4 cells, compared to uninfected MT4 cell controls. IC 50  was calculated by RoboSage, Automated Curve Fitting Program, version 5.00, 10 Jul. 1995. 
     For each assay plate, the results (relative fluorescence units, rfU, or OD values) of wells containing uninfected cells or infected cells with no compound were averaged, respectively. For measurements of compound-induced cytotoxicity, results from wells containing various compound concentrations and uninfected cells were compared to the average of uninfected cells without compound treatment. Percent of cells remaining is determined by the following formula: 
       Percent of cells remaining=(compound-treated uninfected cells, rfU, or OD values/untreated uninfected cells)×100.
 
     A level of percent of cells remaining of 79% or less indicates a significant level of direct compound-induced cytotoxicity for the compound at that concentration. When this condition occurs the results from the compound-treated infected wells at this concentration are not included in the calculation of EC 50 . 
     For measurements of compound antiviral activity, results from wells containing various compound concentrations and infected cells are compared to the average of uninfected and infected cells without compound treatment. Percent inhibition of virus is determined by the following formula: 
       Percent inhibition of virus=(1−((ave. untreated uninfected cells−treated infected cells)/(ave. untreated uninfected cells−ave. untreated infected cells)))×100.
 
     Results: 
     Compounds of the present invention have anti-HIV activity in the range EC 50 =1-1000 nM. 
     Table 3 
     Table 3 shows EC 5 o values for representative compounds of Table 2 after the HIV MT4 Antiviral Cell Assay of Example 84. 
     
       
         
           
               
               
               
             
               
                   
               
               
                 Example number 
                 EC 50  NL4-3 wt (nM) 
                 EC 50  V370A (nM) 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                 1 
                 41.1 
                 34.6 
               
               
                 2 
                 5.2 
                 4.7 
               
               
                 3 
                 61.7 
                 242.7 
               
               
                 4 
                 10.1 
                 9.3 
               
               
                 5 
                 2.6 
                 3.2 
               
               
                 6 
                 0.5 
                 3.8 
               
               
                 7 
                 6.1 
                 23.1 
               
               
                 8 
                 5.9 
                 14.3 
               
               
                 9 
                 12.3 
                 13.5 
               
               
                 10 
                 2.3 
                 2.4 
               
               
                 11 
                 6.3 
                 6.7 
               
               
                 12 
                 4.5 
                 5.1 
               
               
                 13 
                 1.6 
                 2.1 
               
               
                 14 
                 2.1 
                 3.6 
               
               
                 15 
                 6.9 
                 31.1 
               
               
                 16 
                 11.0 
                 7.6 
               
               
                 17 
                 8.7 
                 29.2 
               
               
                 18 
                 0.8 
                 0.7 
               
               
                 19 
                 3.9 
                 4.1 
               
               
                 20 
                 2.3 
                 4.1 
               
               
                 21 
                 76.5 
                 103.5 
               
               
                 22 
                 3.3 
                 3.5 
               
               
                 23 
                 4.9 
                 11.7 
               
               
                 24 
                 40.4 
                 1528.0 
               
               
                 25 
                 14.7 
                 27.8 
               
               
                 26 
                 4.9 
                 545.0 
               
               
                 27 
                 8.2 
                 8.9 
               
               
                 29 
                 17.5 
                 28.6 
               
               
                 31 
                 5.4 
                 22.5 
               
               
                 33 
                 5.3 
                 ND 
               
               
                 34 
                 7.1 
                 21.1 
               
               
                 35 
                 4.2 
                 124.0 
               
               
                 36 
                 4.0 
                 9.4 
               
               
                 37 
                 26.9 
                 556.6 
               
               
                 38 
                 5.2 
                 20.9 
               
               
                 39 
                 6.6 
                 217.1 
               
               
                 40 
                 11.1 
                 18.9 
               
               
                 44 
                 1.8 
                 2.6 
               
               
                 45 
                 1.9 
                 2.5 
               
               
                 46 
                 11.1 
                 64.8 
               
               
                 47 
                 7.7 
                 72.9 
               
               
                 49 
                 21.8 
                 192.3 
               
               
                 50 
                 1.1 
                 1.4 
               
               
                 51 
                 3.5 
                 6.3 
               
               
                 52 
                 4.6 
                 7.0 
               
               
                 53 
                 6.2 
                 494.3 
               
               
                 56 
                 3.4 
                 3.4 
               
               
                 57 
                 4.2 
                 8.0 
               
               
                 58 
                 31.5 
                 379.0 
               
               
                 59 
                 5.3 
                 17.2 
               
               
                 60 
                 60.2 
                 8409.0 
               
               
                 61 
                 129.7 
                 10000.0 
               
               
                 62 
                 103.6 
                 213.2 
               
               
                 63 
                 3.2 
                 5.0 
               
               
                 64 
                 7.2 
                 120.2 
               
               
                 65 
                 83.8 
                 3077.0 
               
               
                 66 
                 9.2 
                 16.1 
               
               
                 67 
                 135.5 
                 411.1 
               
               
                 68 
                 10.5 
                 110.2 
               
               
                 69 
                 1.0 
                 4.1 
               
               
                 70 
                 1.6 
                 2.1 
               
               
                 71 
                 31.2 
                 35.3 
               
               
                 72 
                 5.1 
                 10.8 
               
               
                 73 
                 31.8 
                 62.3 
               
               
                 74 
                 2.9 
                 4.8 
               
               
                 75 
                 0.6 
                 1.0 
               
               
                 76 
                 0.7 
                 0.9 
               
               
                 77 
                 4.7 
                 5.0 
               
               
                 78 
                 2.1 
                 2.7 
               
               
                 79 
                 14.7 
                 76.1 
               
               
                 80 
                 2.4 
                 2.7 
               
               
                 81 
                 3.0 
                 5.4 
               
               
                 82 
                 8.9 
                 70.5 
               
               
                 83 
                 7.3 
                 143.5 
               
               
                   
               
            
           
         
       
     
     Example 86 
     This example shows the EC 50  potencies of Bevirimat, compound 51, compound A, and compound B against wild type HIV, site directed HIV mutants, and clinical HIV isolates. As can be seen in Table 5, compound 51 demonstrates higher potency than the other compounds when measured against HIV among wildtype, certain site directed mutants, and against several clinical HIV isolates. Surprisingly, compound 51 continues to have excellent potency against HIV relative to the other compounds, when protein binding is taken into account as can be seen in Table 6. 
     The results in Tables 5 and 6 for compound 51 demonstrate an unexpected result taken in light of Bevirimat and compounds A and B. The results in the above tables are indicative of the unpredictability in developing anti-HIV compounds where a subtle change in the chemical structure can have a large impact on the clinical result. Where compounds A and B show alternating efficacy in their both their EC 50  values for polymorphism and protein binding, compound 51 shows none of this variability and is therefor unexpectedly superior when viewed in terms of potential clinical applicability. A direct comparison between compound 51 and compounds A and B, demonstrate how subtle chemical substitutions can have a dramatic effect on the in vitro efficacy of a putative anti-HIV compound. 
     A similar advantage for compound 51 can be seen in Table 6 where protein binding and serum shift for the above compounds are compared. This table shows both a significant decrease in the EC 50  and a reduced serum effect for compound 51 relative to the other compounds tested. 
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Wild-type, Polymorphisms, &amp; PBLs—EC 50  (nM) 
               
            
           
           
               
               
               
               
               
               
            
               
                   
                   
                   
                 HIV-1 B 
                 HIV-1 B 
                 HIV-1 C 
               
               
                   
                 MT4 NL.  
                 MT4 NL.  
                 CC1/85 
                 ASJM108 
                 972A009 
               
               
                   
                 wt* 
                 V370A 
                 (V370A) 
                 (V370A+) 
                 (V370A+) 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 Bevirimat 
                 223 
                 6062 
                 &gt;10000 
                 &gt;10000 
                 13435 
               
               
                 Compound A 
                 14 
                 16 
                 5 
                 51 
                 1159 
               
               
                 CompoundB 
                 8 
                 8 
                 13 
                 6 
                 2000 
               
               
                 Compound51 
                 0.8 
                 0.7 
                 1.2 
                 1.0 
                 4 
               
               
                   
               
               
                 *Consensus Clade B Sp1 Genotype. 
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Protein Binding Effects on Potency 
               
            
           
           
               
               
               
               
               
            
               
                   
                   
                   
                 LHIV 
                 Fold shift 
               
               
                   
                   
                 LHIV 
                 IC 50  (nM) 
                 with 40% 
               
               
                   
                   
                 IC 50  (nM) 
                 with 40% HuS 
                 HuS 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 Bevirimat 
                 42 
                 6600 
                 157 
               
               
                   
                 Compound A 
                 9.3 
                 73 
                 7.9 
               
               
                   
                 CompoundB 
                 3.2 
                 43 
                 13.4 
               
               
                   
                 Compound51 
                 1.9 
                 10.6 
                 5.5 a   
               
               
                   
                   
               
               
                   
                   a Definitive protein shift value determined in PBL&#39;s by titration at SRI is 1X 
               
            
           
         
       
     
     Example 87 
     It is suspected that many anti-HIV compounds might potentially be less effective in the treatment of patients who have failed a previous protease inhibitor-containing regimen and whose viruses have developed drug resistance mutations within the protease gene. 
     A major obstacle to their long-term efficacy of anti-HIV therapies has been the emergence of resistance to current antiretroviral drugs. One method for comparing the effectiveness of anti-HIV compounds is the characterization of such compounds&#39; polymorphism at drug resistant sites. 
     A comparison of compound 51 versus Bevirimet (“BVM”) against a broad panel of HIV isolates is shown in  FIG. 1 . From  FIG. 1 , it can be seen that compound 51 shows superior polymorphism coverage across this panel of HIV isolates to Bevirimat. Likewise, compound 51 shows superior polymorphism coverage as compared to compound C as shown in  FIG. 2 . 
     This example was performed as follows. Crude human primary blood mononuclear cells (PBMCs) were collected from healthy donors via leukapheresis and determined to be negative for HIV exposure, among a variety of additional infectious diseases. PBMCs were isolated via Ficoll-Hypaque density gradient separation followed by aspiration of the banded leukocytes from the gradient. Purified PBMCs from 10 individual donors were combined and cryopreserved in liquid nitrogen as a PBMC pool. Various PBMC pools were utilized. 
     Various HIV-1 isolates were obtained from different sources. A stock of the virus was generated in a pool of CD4+ PBMCs and frozen. Viral replication for each virus stock was measured on PHA-blasted PBMC pools to determine the appropriate virus input for anti-viral assays. HIV gag sequences were determined for each isolate by the Sanger di-deoxy sequencing method. 
     Cryo-preserved PBMC pools were thawed and stimulated with 2 μg/mL PHA in media (RPM11640, 20% FBS, 10% T cell growth factor, antibiotics) for 3 days. Blasts were then washed, counted, and cultured in media at 2×10 6  cells/mL in media with 10U/mL recombinant IL-2 for an additional 24 hours. Stimulated PBMCs were counted for trypan blue exclusion viability and seeded in 96-well round bottom plates at a final density of 1×10 6  cells/well. A volume of the drug dose titration was added to the cells followed by a pre-determined amount of virus. The plates were incubated for 7 days at 37° C., 5% CO 2 . to allow for viral replication and inhibition by compound to assess anti-viral potency. 
     In order to determine anti-viral potency, HIV reverse transcriptase activity in cell culture supernatants was measured as a measure of viral replication in the presence or absence of compound. At the end of the incubation period, fifty (50) μL of PBMC culture supernatants were transferred to a new 96-well plate; 10 μL of RT extraction buffer was added followed by the addition of 40 μL of RT assay buffer. The RT plates were thoroughly mixed and placed in a humidified incubator at 37° C., 5% CO 2  for 2 h. DE-81 96-well plates were placed on a vacuum manifold and 100 uL of the RT reaction transferred to the ion-exchange chromatography plates. After applying the solution phase of the RT reaction to the DE-81 plate, the plate was then washed once with prepared 5% Na 2 HPO 4 , followed by a wash of dH 2 0. Plates were then allowed to dry overnight at room temperature. The plates were sealed and 50 μL scintillation fluid added prior to reading on a Topcount (Packard) luminometer at 10s/well. 
     Below are the particular HIV-1 isolates tested as shown in  FIG. 1 :
     1—HIV-1 A_UG275   2—HIV-1 AE_42368   3—HIV-1 C_97/ZA/009   4—HIV-1 C_ETH2220   5—HIV-1 C_ZAM18   6—HIV-1 C_I-2516   7—HIV-1 B_CC1/85   8—HIV-1 B_ASJM108   9—HIV-1 B_SF162   10—HIV-1 B_92US657   11—HIV-1 B_BR92030   12—HIV-1 B_ASM42   13—HIV-1 B_BR92023   14—HIV-1 B_ASM44   15—HIV-1 B_92US660   16—HIV-1 B_THA92014   17—HIV-1 B_IIIB   18—HIV-1 B_301596   19—HIV-1 B_ASM34   20—HIV-1 B_BK132   21—HIV-1 B_ASM57   22—HIV-1 B_ASM54   23—HIV-1 B_92HT599   24—HIV-1 B_CM237   25—HIV-1 B_92HT593   26—HIV-1 B_BZ167   27—HIV-1 B_92US723   

     Below are the particular HIV isolates tested as shown in  FIG. 2 :
     1—HIV-1 A_UG275   2—HIV-1 AE_42368   3—HIV-1 B_301596   4—HIV-1 B_92HT593   5—HIV-1 B_92HT599   6—HIV-1 B_92US657   7—HIV-1 B_92US660   8—HIV-1 B_92US723   9—HIV-1 B_ASJM108   10—HIV-1 B_ASM34   11—HIV-1 B_ASM42   12—HIV-1 B_ASM44   13—HIV-1 B_ASM54   14—HIV-1 B_BK132   15—HIV-1 B_BZ167   16—HIV-1 B_CC1/85   17—HIV-1 B_CM237   18—HIV-1 B_IIIB   19—HIV-1 B_SF162   20—HIV-1 C_97/ZA/009   21—HIV-1 C_ETH2220   22—HIV-1 C_ZAM18   

     Example 88 
     This example shows in Table 7 a direct comparison between compound 51, compound 56, and compound C. Intrinsic HIV wild type potency for compound 51 relative to compound C is about 10× better (0.8 nM vs. 6 nM). In the HIV polymorphic strain, V370, the potency for compound 51 relative to compound C is even more dramatic (0.7 nM vs. 19 nM). In terms of human protein shift listed as human serum (HS) in this example, is another factor of 10 fold better for compound 51 relative to compound C (5.5 fold shift vs. a 48.9 fold shift). 
     All taken together, as can be seen in Table 7 there is a factor of 238 fold improvement for compound 51 over compound C for the C trough  target, which is the PAEC 50  (or PAEC 90  which is the same factor just higher for each by a factor of 3-4 fold). In fact, the reported 5.5 fold shift in Table 7 for 40% human serum in a multicell HIV full life cycle reporter based assay. 
     Table 7 also depicts the protein adjusted EC 50  for the V370A polymorphic virus (which represents up to 40% of patients in Glade B and maybe more outside Glade B) you can see that compound 51 potency is unexpectedly greater at 3.9 nM, while compound C potency is lower at 929 nM. 
     
       
         
           
               
               
               
               
               
             
               
                 TABLE 7 
               
               
                   
               
               
                   
                 HIV w/t  
                 V370A  
                 Human Serum 
                 V370A 
               
               
                 Compound Identity 
                   MT4 EC 50   
                   MT4 EC 50   
                 Fold Shift** 
                 PAEC 50 *** 
               
               
                   
               
             
            
               
                 Compound 51 (′232) 
                 0.8 nM 
                 0.7 nM 
                  5.5X 
                  3.9 nM 
               
               
                 Compound 56 (′233) 
                   4 nM 
                   4 nM 
                 16.2X 
                  65 nM 
               
               
                 Compound C (′163) 
                   6 nM 
                  19 nM 
                 48.9X 
                 929 nM 
               
               
                   
               
               
                  **Human Serum fold shift values determined using 40% human serum in a HIV multicell full life cycle assay. 
               
               
                 ***V370A PAEC 50  is determined by the product of V370A  MT4 EC 50  and human serum fold shift. 
               
            
           
         
       
     
     Example 89 
     The PBL assay in this example was performed as follows in order to study the effect of human serum on the HIV antiviral activity of certain compounds. In particular, the effect of the presence of human serum on the antiviral activity of compound 51 and Bevirimat (“BVM”) was evaluated in a modified PMBC assay. 
     In the standard assay, PHA/IL-2 stimulated PBMCs (5×10 4  cells/well) were incubated with virus and compound 51 or BVM (both compounds tested at range of 0.06 nM to 25 mM) for 7 days. 
     In the modified PBMC assay, PHA/IL-2 stimulated PBMCs were pre-incubated with HIV-1 strain JR-CSF prior to addition of compound 51 or BVM and human serum. In this assay, 8×10 6  cells from pooled donors were incubated with virus for 1 hour, followed by centrifugation for 1 hour. Cells were then gently re-suspended and incubated an additional 2 hours. During this second incubation period, 2.5×10 4  uninfected PBMCs from pooled donors were added to the interior 60 wells of a 96 well plate, followed by the addition of compound 51 or BVM (both compounds tested at range of 0.06 nM to 25 mM) and human serum (10%, 20%, 30% and 40%) to the appropriate wells. At the end of the second incubation period, infected cells were diluted in media (5×10 5  cells/mL) (without washout of virus) and 50 mL (2.5×10 4  cells) were added to each well of the plate and incubated for 7 days. 
     Following incubation in both the standard and modified assays, supernatants were assayed for reverse transcriptase (RT) activity and p24 antigen content by ELISA and spectrophotometric analysis at 450 nM. 
     Compound 51 maintained antiviral activity in the presence of human serum at all serum concentrations tested, with no apparent shift in IC 50  values (range: 0.52 to 3.07 nM) compared to the IC 50  value (0.69 nM) in the standard assay (0% serum). These results indicate that the inhibitory activity of compound 51 is minimally or not at all impacted by serum proteins. Extrapolating from the normalized IC 50  values observed in the presence of the various human serum concentrations gave an estimated IC 50  value of 0.33 nM for 100% human serum (assuming a linear relationship). BVM also maintained antiviral activity in the presence of human serum at all concentrations, however, there was a large increase in the IC 50  values (range: 1.38 mM to 13 mM) associated with increasing human serum concentrations compared to the standard assay (9.76 nM). Extrapolating from the normalized IC 50  values observed in the presence of the various concentrations of human serum there is a 2,310-fold change in the IC 50  value in the presence of 100% serum (compared to the control experiment, suggesting that BVM is highly bound by serum proteins). 
     The effect of human serum on antiviral activity of compound 51 and BVM was also evaluated in the LHIV assay. Using the same format as the standard LHIV assay, the addition of 40% human serum caused a 5.6-fold shift in the IC 50  value (10.6 nM) for compound 51 and a 174.9-fold shift in IC 50  value (3.88 mM) for BVM. 
     However, when determined in a PBL assay containing a titration between 0 and 40% human serum the extrapolated value is 1 fold or less. Therefore, the improvement of compound 51 over compound C, in terms of potency while in the presence of human serum, is likely &gt;1300 fold better when a more typical baseline is taken into account. See  FIGS. 3, 4, and 5 .