Patent Publication Number: US-2020281245-A1

Title: Human milk fortifier

Description:
TECHNICAL FIELD OF THE INVENTION 
     The present invention relates to a human milk fortifier composition, more specifically to a human milk fortifier composition comprising human milk oligosaccharides. In particular the present invention relates to a human milk fortifier composition specifically tailored to fortify the breastmilk of a primiparous woman, and for consumption by an infant or child born who is the offspring of a primiparous mother. The invention furthermore relates to the use of said human milk fortifier composition. 
     BACKGROUND OF THE INVENTION 
     Infants who are the offspring of primiparous woman are considered to be at an increased risk of suffering from a variety of health complaints in infancy, childhood, and later life. Infants born of primiparous mothers are more likely to be smaller and/or thinner at birth, and so at an increased risk of suffering from conditions associated with being small and/or underweight, and at the same time are also more likely to be larger children and suffer from conditions associated with being larger e.g. being overweight or obese—this is because of dramatic catch-up gains in weight and length that overcompensate for their initial size deficit. 
     The reasons for the increased health risks seen in infants who are the offspring of primiparous mothers is not clear. However, there is a need to identify factors that may contribute to this risk and to address them. 
     The inventors have now identified a factor that they believe may contribute to this risk. In particular the inventors have found that the concentration of one or more human milk oligosaccharides (herein after “HMOs”) found in human breast milk (hereinafter “HM”) produced by primiparous mothers, may differ from the concentration found in HM produced by multiparous mothers. More particularly the inventors have found that the concentration of an HMO found in HM produced by primiparous mothers may be lower than the concentration of the same HMO found in HM produced by multiparous mothers. 
     HMOs are, collectively, the third largest solid constituents in human milk, and a variety of benefits have been associated with them, in consequence, an optimal intake of these compounds in infancy and childhood is believed to be necessary to ensure optimum health and development. HMOs have for example been linked to a variety of biological functions including the establishment of gut microbiota. Some HMOs have also been linked to growth and body composition. 
     Accordingly, there is need for milk fortifiers comprising one or more HMO that can be used to fortify HM produced by primiparous mothers, and to optimise the intake of one or more HMO in infants consuming HM from a primiparous mother. 
     SUMMARY OF THE INVENTION 
     The invention is set out in the claims and in the detailed description included herein. The inventors have found that the concentration of an HMO found in HM produced by multiparous mothers may be higher than the concentration of the same HMO found in HM produced by primiparous mothers. In light of this finding, the inventors have developed a human milk fortifier composition comprising one or more HMOs. 
     Said human milk fortifier may be tailored to fortify the breast milk of a primiparous women. 
     The one or more HMO may be a sialylated oligosaccharide, a fucosylated oligosaccharide, an N-acetylated oligosaccharide, or any combination thereof. The one or more HMO may for example be selected from the group consisting of; 2′-Fucosyllactose, 3′-Fucosyllactose, 6′Galactosyllactose, Blood group A Tetrasaccharide, Difucosyllacto-N-Hexose-A, Lacto-N-Difucosylhexose, Lacto-N-Fucosylpentaose-I, Lacto-N-Fucosylpentaose-II, Lacto-N-Fucosylpentaose-III, Lacto-N-Fucosylpentaose-V, Lacto-N-hexaose A, Lacto-N-hexaose B, Lacto-N-Neodifucosylhexose, Lacto-N-Neofucosylpentaose, Lacto-N-Neotetraose, Lacto-N-Tetraose, Lactodifucosyllactose, Sialyllacto-N-Tetraose a, Sialyllacto-N-Tetraose b, and any combination thereof. 
     It may be particularly beneficial if the HMO is selected from the group consisting of; 3′-Fucosyllactose, Lacto-N-Fucosylpentaose, and any combination thereof. 
     The human milk fortifier composition may comprise an HMO in a range of 0.1 to 10000 mg/L. 
     The human milk fortifier may be specifically tailored for up an infant of an age selected from the group consisting of; up to 4 months of age, up to 3 months of age, up to 2 months of age, up to 1 months of age, up to 2 weeks of age, and up to 1 week of age. It may for example be specifically tailored to supplement breastmilk produced for up an infant of up to one month of age or up to 2 weeks of age. The infant may be an infant of a primiaparous woman. 
     The human milk fortifier may further comprise one or more ingredient selected from the group consisting of vitamins, minerals, protein, carbohydrates, and probiotics. 
     Further provided is a method of preparing a human milk fortifier composition tailored to fortify the breast milk of a primiparous women, said method comprising the steps of: measuring out an appropriate amount of a human milk fortifier composition and mixing it with a diluent and/or additive. 
     Also provided is a human milk fortifier as defined herein, for use in fortifying human breast milk and in particular human breastmilk from a primiparous woman. 
     The human milk fortifier as defined herein may to provide an optimised amount of one or more HMO to an infant. The infant may be selected from the group consisting of: preterm infants and term infants. The infant may be an infant who is the offspring of a primiparous mother. 
     Also provided is a nutritional system comprising: 
     a. a human milk fortifier composition tailored to fortify the breast milk of a primiparous women, and b. A human milk fortifier composition, wherein, said human milk fortifier composition tailored to fortify the breast milk of a primiparous women comprises one or more HMO in a concentration higher than in the human milk fortifier composition. The method may also comprise the step of determining whether the woman is a primiaparous woman. 
    
    
     
       DRAWINGS 
         FIG. 1  is a graphical representation of the 2′-fucosyllactose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 2  is a graphical representation of the 3-Fucosyllactose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 3  is a graphical representation of the 6′Galactosyllactose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 4  is a graphical representation of the Blood group A Tetrasaccharide concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 5  is a graphical representation of the Difucosyllacto-N-Hexose-A concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 6  is a graphical representation of the Lacto-N-Difucosylhexose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 7  is a graphical representation of the Lacto-N-Fucosylpentaose-I concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 8  is a graphical representation of the Lacto-N-Fucosylpentaose-II concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 9  is a graphical representation of the Lacto-N-Fucosylpentaose-III concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 10  is a graphical representation of the Lacto-N-Fucosylpentaose-V concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 11  is a graphical representation of the Lacto-N-hexaose A concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 12  is a graphical representation of the Lacto-N-hexaose B concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 13  is a graphical representation of the Lacto-N-Neodifucosylhexose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 14  is a graphical representation of the Lacto-N-Neofucosylpentaose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 15  is a graphical representation of the Lacto-N-Neotetraose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 16  is a graphical representation of the Lacto-N-Tetraose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 17  is a graphical representation of the Lactodifucosyllactose concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 18  is a graphical representation of the Sialyllacto-N-Tetraose a concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
         FIG. 19  is a graphical representation of the Sialyllacto-N-Tetraose b concentration found in HM by delivery mode at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. 
     
    
    
     DETAILED DESCRIPTION 
     In a first aspect of the present invention there is provided a human milk fortifier composition comprising one or more HMO. 
     The term “human milk fortifier composition” as used herein, refers to a nutritional composition for use in combination and in admixture with human breast milk. Unless otherwise specified, the term “human milk fortifier composition” specifically excludes conventional infant formulas that provide the sole or primary source of infant nutrition and that are not typically combined and admixed with human milk to supplement human milk feedings. 
     The term “fortifier” refers to a composition which comprises one or more nutrients having a nutritional benefit for infants, both preterm infants and term infants. The fortifier according to the present invention is rich in HMOs and may therefore be considered as an HMO fortifier, HMO supplement or the like. 
     In an embodiment of the invention, the human milk fortifier composition is tailored/adapted to fortify the breast milk of a primiparous women. A human milk fortifier, as disclosed herein, may be considered as specifically tailored/adapted to fortify the breast milk of a woman who is primaprous if it comprises one or more HMO as described herein. Said human milk fortifier may, for example, comprise said one or more HMO in an amount sufficient to address the deficiency of one or more HMO identified in the human breast milk of primiaparous mothers in comparison to multiparous mothers. A sufficient amount of an HMO may for example be an amount equal to or greater than an amount that an infant born to a multiparous woman would receive, or may for example, be any amount that is equal to or higher than the difference found in the concentration e.g. averages, in human milk produced by primparous women and multiparous women. Said human milk fortifier composition may be a parity specific human milk fortifier i.e. a milk fortifier sold specifically for use in primiparous women e.g. marketed as a being for use to fortify the breastmilk of primiparous women 
     The term “multiparous” as used herein refers to a woman who has given birth more than once or has more than 1 child. 
     The term “primiparous” as used herein refers to a woman who has given birth once, or has only 1 child. 
     The term “infant” as used herein, refers to humans of less than about 1 year of age. The term includes preterm infants, premature infants, small for gestational age (SGA) infants and/or infant with low birth weight (LBW). 
     The terms “preterm infants” or “premature infants” as used herein, refer to infants who were not born at term. Generally they refers to infants born alive prior to 37 weeks of gestation/pregnancy. 
     The term “small for gestational age infant” as used herein, refers to an infant who is smaller in size than normal for their gestational age at birth, most commonly defined as a weight below the 10th percentile for the gestational age. In some embodiments, SGA may be associated with intrauterine growth restriction (IUGR), which refers to a condition in which a foetus is unable to achieve its potential size. 
     The term “low birth weight infants” as used herein refers to an infant that has a body weight under 2500 g at birth. It therefore encompasses:
         infants who have/had a body weight from 1800 to 2500 g at birth (usually called “low birth weight” or LBW)   infants who have/had a body weight from 1000 to 1800 g at birth (called “very low birth weight” or VLBW)   infants who have/had a body weight under 1000 g at birth (called “extremely low birth weight” or ELBW) Infants or young children with low birth weight may or may not be preterm, and similarly, infants or young children who were small for gestational age may or may not be preterm.       

     The term “child” as used herein, refers to humans from about 1 to about 7 year of age, for example, between 1 and 3 years of age. 
     The human milk fortifier composition of the invention may comprise any type of HMO. 
     In an embodiment of the present invention the human milk fortifier comprise a HMO selected from the group consisting of a sialylated oligosaccharide, a fucosylated oligosaccharide, an N-acetylated oligosaccharide, or any combination of the foregoing. 
     The term “sialylated oligosaccharide” as used herein refers to an oligosaccharide having a sialic acid (such as N-acetylneuraminic acid and/or N-glycolylneuraminic acid) residue. 
     The term “N-acetylated” oligosaccharide as used herein refers to an oligosaccharide having at least one hexose carrying an N-acetyl residue. 
     The term “fucosylated oligosaccharide” as used herein refers to an oligosaccharide having a fucose residue. 
     In a more specific embodiment the human milk fortifier composition of the invention comprises an HMO selected from the group consisting of: 2′-Fucosyllactose, 3-Fucosyllactose, 6′Galactosyllactose, Blood group A Tetrasaccharide, Difucosyllacto-N-Hexose-A, Lacto-N-Difucosylhexose, Lacto-N-Fucosylpentaose-I, Lacto-N-Fucosylpentaose-II, Lacto-N-Fucosylpentaose-III, Lacto-N-Fucosylpentaose-V, Lacto-N-hexaose A, Lacto-N-hexaose B, Lacto-N-Neodifucosylhexose, Lacto-N-N eofucosylpentaose, Lacto-N-Neotetraose, Lacto-N-Tetraose, Lactodifucosyllactose, Sialyllacto-N-Tetraose a, Sialyllacto-N-Tetraose b, and any combination thereof. 
     In an even more specific embodiment the human milk fortifier composition of the invention comprises an HMO selected from the group consisting of: 3-Fucosyllactose, Lacto-N-Fucosylpentaose II, 2′-Fucosyllactose and any combination thereof. 
     The human milk fortifier composition of the invention may comprise an HMO in any concentration. 
     In particular the human milk fortifier composition may comprise one an HMO in a concentration of 0.1 to 10000 ring/L e.g. 0.1 to 6000 mg/L e.g. 0.1 to 350 mg/L. 
     The concentrations listed herein may refer to a concentration after a composition has been reconstituted or mixed with water or milk. 
     The human milk fortifier composition of the invention may for example comprise one or more of the HMOs listed in table I in a concentration range listed in table I. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE I 
               
               
                   
                   
               
               
                   
                 HMO 
                 Concentration Range mg/L 
               
               
                   
                   
               
             
            
               
                   
                 2′-Fucosyllactose 
                   9-8500 
               
               
                   
                 3-Fucosyllactose 
                   1-3600 
               
               
                   
                 6′Galactosyllactose 
                 0.09-250  
               
               
                   
                 Blood group A Tetrasaccharide 
                   1-1000 
               
               
                   
                 Difucosyllacto-N-Hexose-A 
                  0.4-1100 
               
               
                   
                 Lacto-N-Difucosylhexose 
                   20-3000 
               
               
                   
                 Lacto-N-Fucosylpentaose-I 
                  0.4-4300 
               
               
                   
                 Lacto-N-Fucosylpentaose-II 
                   32-3600 
               
               
                   
                 Lacto-N-Fucosylpentaose-III 
                  13-800 
               
               
                   
                 Lacto-N-Fucosylpentaose-V 
                 1.5-500 
               
               
                   
                 Lacto-N-hexaose A 
                 0.15-200  
               
               
                   
                 Lacto-N-hexaose B 
                 0.3-120 
               
               
                   
                 Lacto-N-Neodifucosylhexose 
                 0.3-330 
               
               
                   
                 Lacto-N-Neofucosylpentaose 
                 0.1-65  
               
               
                   
                 Lacto-N-Neotetraose 
                 0.1-700 
               
               
                   
                 Lacto-N-Tetraose 
                   4-3400 
               
               
                   
                 Lactodifucosyllactose 
                   2-3300 
               
               
                   
                 Sialyllacto-N-Tetraose a 
                  1.5-1900 
               
               
                   
                 Sialyllacto-N-Tetraose b 
                 0.7-180 
               
               
                   
                   
               
            
           
         
       
     
     In an embodiment of the present invention the human milk fortifier composition of the invention may comprise one or more of the HMOs listed in table II in the concentration range listed in table II. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE II 
               
               
                   
                   
               
               
                   
                 HMO 
                 Concentration Range mg/L 
               
               
                   
                   
               
             
            
               
                   
                 2′-Fucosyllactose 
                  9-350 
               
               
                   
                 3-Fucosyllactose 
                 51-230 
               
               
                   
                 6′Galactosyllactose 
                 0.09-11   
               
               
                   
                 Blood group A Tetrasaccharide 
                 1.5-22   
               
               
                   
                 Difucosyllacto-N-Hexose-A 
                 0.4-9   
               
               
                   
                 Lacto-N-Difucosylhexose 
                 20-160 
               
               
                   
                 Lacto-N-Fucosylpentaose-I 
                 0.4-75   
               
               
                   
                 Lacto-N-Fucosylpentaose-II 
                 14-190 
               
               
                   
                 Lacto-N-Fucosylpentaose-III 
                 13-16  
               
               
                   
                 Lacto-N-Fucosylpentaose-V 
                 1.5-22   
               
               
                   
                 Lacto-N-hexaose A 
                 2-4  
               
               
                   
                 Lacto-N-hexaose B 
                  1-4.5 
               
               
                   
                 Lacto-N-Neodifucosylhexose 
                 1.1-9   
               
               
                   
                 Lacto-N-Neofucosylpentaose 
                 1.8-6   
               
               
                   
                 Lacto-N-Neotetraose 
                 0.1-6   
               
               
                   
                 Lacto-N-Tetraose 
                 23-125 
               
               
                   
                 Lactodifucosyllactose 
                 2-38 
               
               
                   
                 Sialyllacto-N-Tetraose a 
                 1.5-36   
               
               
                   
                 Sialyllacto-N-Tetraose b 
                 0.7-11   
               
               
                   
                   
               
            
           
         
       
     
     The human milk fortifier of the invention may be tailored to fortify breastmilk produced for an infant or child of any age e.g. any age born to a primparous mother 
     In an embodiment of the present invention the human milk fortifier composition is tailored/adapted to fortify breastmilk produced for an infant of an age selected from the group consisting of; up to 4 months of age, up to 3 months of age, up to 2 months of age, up to 1 months of age, up to 2 weeks of age, up to 1 week of age. For example the human milk fortifier composition may be tailored/adapted to fortify breastmilk produced for an infant up to 1 month of age e.g. up to 2 weeks of age. The infant may be born to a primparous mother. 
     In an embodiment of the present invention the human milk fortifier is tailored/adapted for an infant of up to 1 month of age e.g. an infant up to 2 weeks of age, or an infant up to 1 week of age and said composition comprises one or more HMO selected from the group consisting of 3-Fucosyllactose, Lacto-N-Fucosylpentaose II, 2′-Fucosyllactose, and any combination thereof. In a more specific embodiment said HMOs, if present in said human milk fortifier tailored/adapted for an infant of up to 1 month of age, may be present in a concentration range as set out in table III. 
     
       
         
           
               
               
               
             
               
                 TABLE III 
               
               
                   
               
               
                   
                 HMO 
                 Concentration Range mg/L 
               
               
                   
               
             
            
               
                   
                 3-Fucosyllactose 
                 51-230 
               
               
                   
                 Lacto-N-Fucosylpentaose II 
                 14-190 
               
               
                   
                 2′-Fucosyllactose 
                  9-350 
               
               
                   
               
            
           
         
       
     
     The human milk fortifier composition of the invention can also comprise any other ingredients or excipients known to be employed in human milk fortifier compositions. 
     Non limiting examples of such ingredients include: proteins, amino acids, carbohydrates, lipids, prebiotics or probiotics, essential fatty acids, nucleotides, nucleosides, vitamins, minerals and other micronutrients. 
     In an embodiment of the invention the human milk fortifier composition further comprises one or more ingredient selected from the group consisting of vitamins, minerals, protein, carbohydrates, and probiotics. 
     Non limiting examples of proteins include: casein, alpha-lactalbumin, whey, soy protein, rice protein, corn protein, oat protein, barley protein, wheat protein, rye protein, pea protein, egg protein, sunflower seed protein, potato protein, fish protein, meat protein, lactoferrin, serum albumin, immunoglobins, and combinations thereof. 
     Non limiting examples of amino acids include leucine, threonine, tyrosine, Isoleucine, arginine, alanine, histidine, isoleucine, proline, valine, cysteine, glutamine, glutamic acid, glycine, serine, arginine, lysine, methionine, phenylalanine, tryptophane, asparagine, aspartic acid, and combinations thereof. 
     Non limiting examples of carbohydrates include lactose, saccharose, maltodexirin, starch, and combinations thereof. 
     Non limiting examples of lipids include: palm olein, high oleic sunflower oil, high oleic safflower oil, canola oil, fish oil, coconut oil, bovine milk fat, and combinations thereof. 
     Non limiting examples of essential fatty acids include: linoleic acid (LA), a-linolenic acid (ALA) and polyunsaturated fatty acids (PUFAs). The gender specific synthetic nutritional compositions of the invention may further contain gangliosides monosialoganglioside-3 (GM3) and disialogangliosides 3 (GD3), phospholipids such as sphingomyelin, phospholipids phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and combinations thereof. 
     None limiting examples of prebiotics include: oligosaccharides (other than HMOs) optionally containing fructose, galactose, mannose; dietary fibers, in particular soluble fibers, soy fibers; inulin; and combinations thereof. Preferred prebiotics are fructo-oligosaccharides (FOS), galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMO), xylo-oligosaccharides (XOS), arabino-xylo oligosaccharides (AXOS), mannan-oligosaccharides (MOS), oligosaccharides of soy, glycosylsucrose (GS), lactosucrose (LS), lactulose (LA), palatinose-oligosaccharides (PAO), malto-oligosaccharides, gums and/or hydrolysates thereof, pectins and/or hydrolysates thereof, and combinations of the foregoing. 
     Non limiting examples of probiotics include:  Bifidobacterium, Lactobacillus, Lactococcus, Enterococcus, Streptococcus, Kluyveromyces, Saccharoymces, Candida , in particular selected from the group consisting of  Bifidobacterium longum, Bifidobacterium lactis, Bifidobacterium animalis, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus paracasei, Lactobacillus salivarius, Lactobacillus lactis, Lactobacillus rhamnosus, Lactobacillus johnsonii, Lactobacillus plantarum, Lactobacillus salivarius, Lactococcus lactis, Enterococcus faecium, Saccharomyces cerevisiae, Saccharomyces boulardii  or mixtures thereof, preferably selected from the group consisting of  Bifidobacterium longum  NCC3001 (ATCC BAA-999),  Bifidobacterium longum  NCC2705 (CNCM I-2618),  Bifidobacterium longum  NCC490 (CNCM I-2170),  Bifidobacterium lactis  NCC2818 (CNCM I-3446),  Bifidobacterium breve  strain A,  Lactobacillus paracasei  NCC2461 (CNCM I-2116),  Lactobacillus johnsonii  NCC533 (CNCM I-1225),  Lactobacillus rhamnosus  GG (ATCC53103),  Lactobacillus rhamnosus  NCC4007 (CGMCC 1.3724),  Enterococcus faecium  SF 68 (NCC2768; NCIMB10415), and combinations thereof. 
     Non limiting examples of Nucleotides include: cytidine monophosphate (CMP), uridine monophosphate (UMP), adenosine monophosphate (AMP), guanosine monophosphate (GMP), and combinations thereof. 
     Non limiting examples of vitamins and minerals include: vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin Bi2, vitamin E. vitamin K. vitamin C, vitamin D, folic acid, inositol, niacin, biotin, pantothenic acid, choline, calcium, phosphorous, iodine, iron, magnesium, copper, zinc, manganese, chloride, potassium, sodium, selenium, chromium, molybdenum, taurine, L-carnitine, and combinations thereof. Minerals are usually added in salt form. 
     Other suitable and desirable ingredients of human milk fortifier compositions, that may be employed in the human milk fortifier compositions of the invention, are described in guidelines issued by the Codex Alimentarius. 
     The human milk fortifier composition of the invention may be prepared in any way known in the art to prepare human milk fortifier compositions. It is well within the purview of the skilled person to decide on a method depending on the type of human milk fortifier in question e.g. powder or liquid. An exemplary method for preparing a human milk fortifier in accordance with the invention is set out below. 
     A human milk fortifier may be prepared, for example, by blending together lipid, protein, HMOs, and carbohydrate in appropriate proportions. If used, emulsifiers may be included in the blend at this stage. The vitamins and minerals may be added at this stage but are usually added later to avoid thermal degradation. Any lipophilic vitamins, such as vitamin A, D, E and K, and emulsifiers may be dissolved into the fat source prior to blending. Water, preferably water which has been subjected to reverse osmosis, may then be mixed in to a liquid mixture. 
     The liquid mixture may then be thermally treated to reduce bacterial loads. For example the liquid mixture may be rapidly heated to a temperature on the range of about 80° C. to about 110° C. for about 5 seconds to about 5 minutes. This may be carried out by steam injection or by heat exchanger, for example a plate heat exchanger. 
     The liquid mixture may then be cooled to about 60° C. to about 85° C., for example by flash cooling. The liquid mixture may then be homogenised, for example in two stages at about 7 MPa to about 40 MPa in the first stage and about 2 MPa to about 14 MPa in the second stage. The homogenised mixture may then be further cooled and any heat sensitive components, such as vitamins and minerals may be added. The pH of the homogenised mixture is conveniently standardised at this point. 
     The homogenized liquid mixture is then filled into suitable containers, preferably aseptically. However, the liquid composition may also be reported in the container. Suitable apparatus for carrying out filling of this nature is commercially available. 
     A human milk fortifier composition specifically tailored/adapted to fortify the breast milk of a primiparous women may be prepared from a human milk fortifier composition e.g. a human milk fortifier composition not specifically tailored to fortify the breast milk of a woman of a particularly parity e.g. primiparous or multiparous. 
     Accordingly, in another aspect of the present invention there is provided a method of preparing a human milk fortifier composition tailored to fortify the breast milk of a primiparous women, said method comprising the steps of: measuring out an appropriate amount of a human milk fortifier composition e.g. a human milk fortifier composition not specifically tailored to fortify the breast milk of a woman of a particular parity, and mixing it with an additive and/or a diluent e.g. one or more HMOs and/or water, so as to arrive at a human milk fortifier composition tailored to fortify the breast milk of a primiparous woman in accordance with the invention. 
     The additive may be a one or more HMO e.g. one or more HMO in a concentration such, that that when the additive is mixed with a human milk fortifier composition, and optionally a diluent, the resulting mixture is a human milk fortifier tailored to fortify the breast milk of a primiparous women, in accordance with the invention. 
     The additive may be a parity specific additive e.g. an additive marketed as specifically being for use by primiparous women. 
     In another aspect of the present invention there is provided a human milk fortifier in accordance with the invention, for use in fortifying human breast milk. 
     In an embodiment the human breastmilk is breastmilk from primiparous women. 
     In another aspect of the present invention there is provided a human milk fortifier composition in accordance with the invention, for use to provide an optimised amount of and/or to prevent a sub-optimal intake of one or more HMO to an infant or child who is the offspring of a primiparous mother. 
     An optimised amount of one or more HMO would be an amount equal to or greater than an amount e.g. the average amount, that an infant born to a multiparous woman would be considered to receive e.g. an amount of an HMO set out in table I, II or III included herein. 
     In another aspect of the present invention there is provided a human milk fortifier composition in accordance with the invention, for use in optimising the health and development and/or preventing the sub-optimal health and development e.g. growth and development of an infant or child who is the offspring of a primiparous mother. 
     The human milk fortifier compositions of the invention may not only optimise the health and development of an infant or child who is the offspring of a primiparous mother in the short term, but may also do so in the long term. 
     In another aspect of the present invention there is provided a human milk fortifier composition in accordance with the invention, for use in optimising the gut microbiota and/or preventing sub-optimal gut microbiota in an infant or child who is the offspring of a primiparous mother. HMOs are known to be important for the establishment of gut microbiota and therefore an optimal supply of HMOs may lead to an optimised gut microbiota. 
     In another aspect of the present invention there is provided the use of a human milk fortifier composition in accordance with the invention, in the manufacture of a composition for use in optimising the gut microbiota and/or health and development of an infant or child who is the offspring of a primiparous mother. 
     A non-optimal gut microbiota may be one showing presence of one or several pathobionts and/or opportunistic pathobionts and/or their toxins and/or virulence factors and/or antibiotic resistence genes. An optimal gut microbiota may be one not showing presence of one or several pathobionts and/or opportunistic pathobionts and/or their toxins and/or virulence factors and/or antibiotic resistence genes. 
     The human milk fortifier compositions of the invention may not only optimise gut microbiota composition and/or health and development short term, but may also do so in the long term. 
     Long term effects may only be evident in months or years e.g. 6 months, 9 months, 12 months, 5 years, 10 years, or 20 years 
     In another aspect of the present invention there is provided the use of a human milk fortifier composition in accordance with the invention, to fortify human breast milk and to improve/prevent sub-optimal breastmilk quality wherein, said breastmilk is from a primiparous women. 
     The quality of breastmilk in a primparous woman may be considered sub-optimal if it comprises one or more HMO in a concentration less than that found in breastmilk from a multiparous woman e.g. in a concentration less than the average found in multiparous women. 
     In another aspect of the present invention there is provided the use of a human milk fortifier according to the invention in optimising and/or preventing the sub-optimal health and development and/or the gut flora composition in an infant or child who is the offspring of a primiparous mother. 
     As stated previously, some HMOs have also been linked to growth and body composition. In particular the intake of some HMOs have been positively linked to weight, lean mass and fat mass. In particular Lacto-N-Fucosylpentaose-II has been linked to increased size and in particular increased fat mass. 
     In another aspect of the present invention there is provided a human milk fortifier according to the invention for use in optimising and/or preventing the sub-optimal growth of an infant or child who is the offspring of a primiparous mother. In an embodiment the human milk fortifier according to the invention comprises Lacto-N-Lacto-N-Fucosylpentaose-II. In a more specific embodiment the human milk fortifier is tailored/adapted to fortify the human breast milk produced for an infant of up to 4 months of age, more specifically up to 1 month of age e.g. up to 2 weeks of age. 
     An infants or child&#39;s growth and development may be optimised if for example said infants or child&#39;s weight and length is within growth curves developed by the WHO. 
     Optimized growth refers to the criteria set by the WHO and they are depicted as charts of the growth of breastfed infants with the appropriate age- and sex-specific weight, length and head circumference. The WHO growth charts are international standards that show how healthy infants should grow, describing the growth of infants living in environments believed to support optimal growth. 
     If an infant or child&#39;s weight and length is within the growth curves developed by the WHO, and he/she has an appropriate distribution of lean and fat mass, it may be assumed that this child/infant is growing and developing in a healthy way, and may have a lower risk for later adverse metabolic outcomes. 
     Health and development and/or gut flora composition and/or growth may be optimised short term or long term. 
     A human milk fortifier tailored to fortify the breastmilk of a primiparous woman may be included in a nutritional system. 
     The term “nutritional system” as used herein refers to a collection of more than one synthetic nutritional compositions advertised or sold as part of the same product range e.g. a collection of human milk fortifiers and/or infant formulas sold under the same brand and adapted/tailored to the nutritional needs of infants born to different parity mothers e.g. primiparous or multiparous mothers. The synthetic nutritional compositions making up the nutritional system may be packaged individually e.g. in capsules or boxes. Said packages can be sold individually, grouped together e.g. wrapped by plastic film or combined in a box, or in a combination of these two ways. The nutritional system may also comprise synthetic nutritional compositions for children older than 12 months. 
     In a further aspect of the present invention there is provided a nutritional system comprising:
         a. a human milk fortifier composition tailored to fortify the breast milk of a primiparous women, in accordance with the invention, and   b. a human milk fortifier composition e.g. a human milk fortifier composition not specifically tailored to fortify the breast milk of a woman of a specific parity or specifically tailored to fortify the offspring of a multiparous woman,       

     wherein, 
     said human milk fortifier composition tailored to fortify the breast milk of a primiparous women comprises one or more HMO in a concentration higher than in the human milk fortifier composition e.g. human milk fortifier composition not specifically tailored to fortify the breast milk of a woman of a specific parity. 
     The concentration of one or HMO in the human milk fortifier tailored for a primiparous woman may be higher by any amount. 
     In an embodiment the human milk fortifier composition tailored for a primiparous woman comprises one or more HMO listed in table II in a higher amount. The higher amount may be an amount within the range given in table II for the HMO in question. 
     In a more specific embodiment the human milk fortifier composition tailored for a multiparous woman comprises one or more HMO listed in table III in a higher amount. The higher amount may be an amount within the range given in table III for the HMO in question. 
     It should be appreciated that all features of the present invention disclosed herein can be freely combined and that variations and modifications may be made without departing from the scope of the invention as defined in the claims. Furthermore, where known equivalents exist to specific features, such equivalents are incorporated as if specifically referred to in this specification. 
     There now follows a series of non-limiting examples that serve to illustrate the invention. 
     EXAMPLES 
     Example 1 
     Longitudinal Clinical Trial: 
     The present inventors designed a longitudinal clinical trial with lactating mothers with milk sampling at 2 days (V1), 17 days (V2), 30 days (V3) 60 days (V4), 90 days (V5), and 120 days (V6) postpartum. The milk samples were quantitatively analyzed for HMOs. 
     The data presented here is from a multi-center, exploratory study with the primary objective of characterizing key nutrient components in human breast milk. Healthy women of any ethnicity having decided to exclusively breast-feed their new born infant from birth to 4 months of infant&#39;s age were recruited during the last 3 months of pregnancy, and their infants were followed up until 4 months of age. 
     Breast milk samples were collected from the mother at the following days postpartum: 0-3 (V1), 17±3 (V2), 30±3 (V3), 60±5 days (V4), 90±5 days (V5) and 120±5 days (v6). Samples were collected after full expression from one breast using a milk pump (Symphony Breastpump, Medela), while the baby was fed on the other breast to produce a satisfactory let-down. All efforts were made to collect complete feed that included fore-, mid-, and hind-milk as a representation of one feed and to avoid within feed variation of lipid and other nutrient contents. Approximately 30 mL aliquot was separated into two conical 15 mL polypropylene tubes for analysis and the rest was returned to the mother to feed the infant. Samples collected for research were stored at −80° C. and shipped on dry ice for analyses to the Nestlé Research Center, Lausanne, Switzerland. 
     Information on parity (primiparous versus multiparous) were collected along with other maternal sociodemographic and anthropometric characteristics. The concentrations of HMOs were measured in breast milk at all the time points as described below. 
     HMO were analysed by ulta high performance liquid chromatography (UHPLC) with fluorescence detection (FLD) after labelling with anthranilamide (2AB). Milk samples (50 μL) or HMO standard solutions (50 μL) were mixed with laminaritriose solution (0.5 μmol/mL; 50 μL) used as internal standard. 2AB labelling solution (2AB, 0.35 mol/L+sodium cyanoborohydride, 1.0 mol/L in DMSO containing 30% acetic acid; 200 μL) was added and the solution heated at 65° C. for 2 h. After 2 h the samples (and standards) were cooled to 4° C. for 10 min and diluted with a solution of acetonitrile/water (75/25; 600 μL). After mixing well, the solutions were placed in a centrifuge (10000×g; 5 min) to remove particulates and the supernatant transferred to vials suitable for the UHPLC autosampler. The HMO were separated on a Waters BEH Glycan column (2.1×150 mm, 1.7 μm), preceded by a Waters BEH Amide Pre-column (2.1×5.0 mm, 1.7 μm) plumbed in to the system in such a way to act as a trapping column for removal of the excess labelling reagents (previously described by Benet &amp; Austin, 2011) using the gradient described below. The 2AB-labelled oligosaccharides were detected by monitoring their fluorescence using λex=330 nm and λem=420 nm. 
     Quantification was performed against standards of the genuine HMO for 2′FL, 3FL, A-tetrasaccharide, 3′SL, 6′SL, LNT, LNnT, LNFP-I, LNFP-V, and LNnFP. All other HMO were quantified against maltotriose assuming an equimolar response of the 2AB-labelled oligosaccharides. 
     The following conditions were used for Separation of HMO on a BEH Glycan Column: 
     
       
         
           
               
               
               
               
               
            
               
                   
               
               
                 Time 
                   
                 Mobile phase* 
                 10 ports valve 
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 (min) 
                 Flow (mL/min) 
                 % A 
                 % B 
                 position 
                 Comment 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                 0.0 
                 0.5 
                 95.0 
                 5.0 
                 10-1  
                 Inject 5.0 μL 
               
               
                   
                   
                   
                   
                   
                 Sample loading on trapping col. 
               
               
                 2.3 
                 0.5 
                 95.0 
                 5.0 
                 10-1  
                 Switch valve - start acquisition 
               
               
                 2.5 
                 0.5 
                 90.0 
                 10.0 
                 1-2 
                   
               
               
                 4.9 
                 0.5 
                 90.0 
                 10.0 
                 1-2 
                 Elution 
               
               
                 32.1 
                 0.5 
                 82.0 
                 18.0 
                 1-2 
                   
               
               
                 48.1 
                 0.5 
                 80.5 
                 19.5 
                 1-2 
                   
               
               
                 61.5 
                 0.5 
                 78.0 
                 22.0 
                 1-2 
                   
               
               
                 89.0 
                 0.5 
                 74.6 
                 25.4 
                 1-2 
                   
               
               
                 89.5 
                 0.4 
                 30.0 
                 70.0 
                 1-2 
                 Washing analytical col. 
               
               
                 92.0 
                 0.4 
                 30.0 
                 70.0 
                 1-2 
                   
               
               
                 93.0 
                 0.4 
                 90.0 
                 10.0 
                 1-2 
                 Re-equilibrate analytical col. 
               
               
                 98.0 
                 0.5 
                 90.0 
                 10.0 
                 10-1  
                 Autozero/switch valve/ 
               
               
                   
                   
                   
                   
                   
                 stop acquisition 
               
               
                 99.0 
                 0.5 
                 95.0 
                 5.0 
                 10-1  
                 Equilibrate trapping col. 
               
               
                 99.5 
                 0.5 
                 95.0 
                 5.0 
                 10-1  
                 End 
               
               
                   
               
            
           
         
       
     
     Benet, T. &amp; Austin, S. (2011) On-line clean-up for 2-aminobenzamide-labeled oligosaccharides, Anal. Chem. 414: 166-168. http://dx.doi.org/10.1016/j.ab.2011.03.002. 
     The results of the compositional analysis were then subject to a statistical analysis. 
     A linear mixed model was used to model each HMO in which visit, parity, country and interaction between visit and parity were used as fixed effects. The within subject variability due to longitudinal repeat measures were taken care of in the model by declaring subject as a random effect. 
     The following statistical model was employed: 
     HMO ˜ Timepoint*Parity+Country+e 
     Timepoint, Parity and Country refers to the fixed effects of the model and takes into consideration the interactions between timepoint and parity. e refers to the random effect of the model which controls for within subject variability. 
     The results of the Statistical analysis (statistical inference) are show in in tables IV-XXIV and  FIGS. 1 to 19 . P value tables are given in the table beneath the compound and results to which they refer. 
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE IV 
               
               
                   
               
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 2′ - 
                 mg/L 
                 UNIQUE 
                 V1 
                 138 
                 4006.75 
                 1517.06 
                 3767.15924 
                 3080.24 
                 4761.75 
                 175.61 
                 9589.39 
               
               
                 Fucosyllactose 
               
               
                 2′ - 
                 mg/L 
                 UNIQUE 
                 V2 
                 171 
                 2715.34 
                 976.99 
                 2715.344 
                 2018.57 
                 3409.34 
                 88.07 
                 5848.58 
               
               
                 Fucosyllactose 
               
               
                 2′ - 
                 mg/L 
                 UNIQUE 
                 V3 
                 149 
                 2517.85 
                 873 
                 2442.034 
                 1959.83 
                 3128.59 
                 325.18 
                 4409.01 
               
               
                 Fucosyllactose 
               
               
                 2′ - 
                 mg/L 
                 UNIQUE 
                 V4 
                 134 
                 2122.26 
                 813.22 
                 2005.177885 
                 1565.84 
                 2658.09 
                 189.51 
                 4026.61 
               
               
                 Fucosyllactose 
               
               
                 2′ - 
                 mg/L 
                 UNIQUE 
                 V5 
                 129 
                 1819.24 
                 721.8 
                 1706.086 
                 1335.71 
                 2347.2 
                 125.08 
                 3820.31 
               
               
                 Fucosyllactose 
               
               
                 2′ - 
                 mg/L 
                 UNIQUE 
                 V6 
                 122 
                 1628.42 
                 675.81 
                 1526.19981 
                 1188.35 
                 2071.5 
                 124.18 
                 3608.3 
               
               
                 Fucosyllactose 
               
               
                 2′ - 
                 mg/L 
                 WITH 
                 V1 
                 48 
                 4386.09 
                 1736.73 
                 4289.65 
                 3228.69 
                 5129.99 
                 1100.93 
                 9498.58 
               
               
                 Fucosyllactose 
                   
                 SIBLINGS 
               
               
                 2′ - 
                 mg/L 
                 WITH 
                 V2 
                 54 
                 2587.29 
                 918.13 
                 2426.2 
                 2041.13 
                 3043.69 
                 533.88 
                 5307.33 
               
               
                 Fucosyllactose 
                   
                 SIBLINGS 
               
               
                 2′ - 
                 mg/L 
                 WITH 
                 V3 
                 52 
                 2442.11 
                 899.71 
                 2320.87 
                 1799.47 
                 2927.19 
                 399.74 
                 4485.03 
               
               
                 Fucosyllactose 
                   
                 SIBLINGS 
               
               
                 2′ - 
                 mg/L 
                 WITH 
                 V4 
                 50 
                 2110.74 
                 738.82 
                 2022.34 
                 1553.69 
                 2605.41 
                 295.32 
                 3824.97 
               
               
                 Fucosyllactose 
                   
                 SIBLINGS 
               
               
                 2′ - 
                 mg/L 
                 WITH 
                 V5 
                 48 
                 1894.55 
                 712.41 
                 1817.36 
                 1381.61 
                 2359.19 
                 295.69 
                 3621.32 
               
               
                 Fucosyllactose 
                   
                 SIBLINGS 
               
               
                 2′ - 
                 mg/L 
                 WITH 
                 V6 
                 46 
                 1651.26 
                 625.97 
                 1621.59 
                 1210.65 
                 2096.49 
                 159.25 
                 2986.33 
               
               
                 Fucosyllactose 
                   
                 SIBLINGS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE IVa 
               
               
                   
                   
               
               
                   
                 Visit 
                 Estimate 
                 SE 
                 pvalue 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 V1 
                 382.33277 
                 163.9708 
                 0.01989229 
               
               
                   
                 V2 
                 -134.65750 
                 159.1302 
                 0.39761651 
               
               
                   
                 V3 
                 -141.87577 
                 161.5152 
                 0.37991228 
               
               
                   
                 V4 
                 -152.21420 
                 163.7197 
                 0.35271384 
               
               
                   
                 V5 
                 -90.77581 
                 165.3151 
                 0.58312380 
               
               
                   
                 V6 
                 -169.04976 
                 166.9656 
                 0.31152596 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE V 
               
               
                   
               
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 3 - 
                 mg/L 
                 UNIQUE 
                 V1 
                 172 
                 385.4664991 
                 415.5565524 
                 237.86708 
                 148.2090175 
                 398.264 
                 11.41228 
                 2468.566 
               
               
                 Fucosyl- 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 UNIQUE 
                 V2 
                 214 
                 553.0388569 
                 521.3096535 
                 354.48191 
                 210.854 
                 649.0720075 
                 43.78025 
                 2537.227 
               
               
                 Fucosyl- 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 UNIQUE 
                 V3 
                 189 
                 674.7012092 
                 574.5232909 
                 478.311 
                 275.97259 
                 831.444 
                 57.45987 
                 2921.70036 
               
               
                 Fucosyl- 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 UNIQUE 
                 V4 
                 173 
                 928.8239356 
                 668.6906753 
                 708.247 
                 454.831 
                 1209.657 
                 81.083 
                 3278.66994 
               
               
                 Fucosyl- 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 UNIQUE 
                 V5 
                 166 
                 1086.633496 
                 757.0840296 
                 864.07916 
                 571.399 
                 1410.950658 
                 93.05087 
                 5715.55268 
               
               
                 Fucosyl- 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 UNIQUE 
                 V6 
                 158 
                 1160.839661 
                 682.5011962 
                 998.615 
                 621.92175 
                 1606.707478 
                 112.962 
                 3443.91 
               
               
                 Fucosyl- 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 519.4932994 
                 531.8511225 
                 289.8366 
                 169.507215 
                 728.1158025 
                 32.664 
                 2078.125 
               
               
                 Fucosyl- 
                   
                 SIBLINGS 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 WITH 
                 V2 
                 75 
                 711.2907307 
                 628.9001769 
                 441.16265 
                 299.365935 
                 939.567185 
                 40.313 
                 2638.36113 
               
               
                 Fucosyl- 
                   
                 SIBLINGS 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 WITH 
                 V3 
                 71 
                 839.9917762 
                 678.0150354 
                 633.44927 
                 345.148815 
                 1173.1234 
                 58.9511 
                 2670.76 
               
               
                 Fucosyl- 
                   
                 SIBLINGS 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 WITH 
                 V4 
                 69 
                 1073.123048 
                 742.4445881 
                 937.19072 
                 548.39844 
                 1322.12 
                 78.17999 
                 2963.532 
               
               
                 Fucosyl- 
                   
                 SIBLINGS 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 WITH 
                 V5 
                 67 
                 1271.12714 
                 815.3119729 
                 1038.34043 
                 672.70648 
                 1623.508555 
                 84.97374 
                 3499.62987 
               
               
                 Fucosyl- 
                   
                 SIBLINGS 
               
               
                 lactose 
               
               
                 3 - 
                 mg/L 
                 WITH 
                 V6 
                 65 
                 1327.515428 
                 787.8852486 
                 1196.455 
                 757.36606 
                 1674.172 
                 109.34912 
                 3653.15978 
               
               
                 Fucosyl- 
                   
                 SIBLINGS 
               
               
                 lactose 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE VI 
               
               
                   
                   
               
               
                   
                 Visit 
                 Estimate 
                 SE 
                 pvalue 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                 V1 
                 1.304577 
                 1.116104 
                 0.01561971 
               
               
                   
                 V2 
                 1.270724 
                 1.114634 
                 0.02742312 
               
               
                   
                 V3 
                 1.249569 
                 1.115363 
                 0.04146459 
               
               
                   
                 V4 
                 1.189985 
                 1.1157696 
                 0.11261200 
               
               
                   
                 V5 
                 1.180027 
                 1.116120 
                 0.13207376 
               
               
                   
                 V6 
                 1.169900 
                 1.116435 
                 0.15445449 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
             
               
                 TABLE VII 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
                 q1.25% 
               
               
                   
               
               
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V1 
                 173 
                 128.9940719 
                 47.46982 
                 126.3577251 
                 100.3019356 
               
               
                 Galactosyllactose 
               
               
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V2 
                 214 
                 40.43950928 
                 34.32453 
                 34.93363996 
                 27.84100032 
               
               
                 Galactosyllactose 
               
               
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V3 
                 190 
                 25.44688677 
                 9.806509 
                 23.17565201 
                 19.42520304 
               
               
                 Galactosyllactose 
               
               
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V4 
                 173 
                 17.59295944 
                 8.05038 
                 15.74108242 
                 12.47470354 
               
               
                 Galactosyllactose 
               
               
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V5 
                 161 
                 14.34098883 
                 7.479945 
                 12.4716807 
                 10.16207027 
               
               
                 Galactosyllactose 
               
               
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V6 
                 147 
                 13.07171018 
                 12.49064 
                 10.80183408 
                 8.87243724 
               
               
                 Galactosyllactose 
               
               
                 6′ - 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 138.9473035 
                 46.10431 
                 137.0616097 
                 106.2274385 
               
               
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                 6′ - 
                 mg/L 
                 WITH 
                 V2 
                 74 
                 39.1280561 
                 15.2604 
                 35.89756199 
                 29.57195798 
               
               
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                 6′- 
                 mg/L 
                 WITH 
                 V3 
                 71 
                 26.40557425 
                 10.78379 
                 24.87935762 
                 20.45856916 
               
               
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                 6′ - 
                 mg/L 
                 WITH 
                 V4 
                 67 
                 18.19156281 
                 9.872754 
                 16.27389683 
                 12.55895567 
               
               
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                 6′ - 
                 mg/L 
                 WITH 
                 V5 
                 65 
                 14.43524793 
                 7.081825 
                 12.01385773 
                 9.826798342 
               
               
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                 6′ - 
                 mg/L 
                 WITH 
                 V6 
                 61 
                 12.24780197 
                 5.723957 
                 10.93564962 
                 9.250831953 
               
               
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 param 
                 unit 
                 DELITYP 
                 visit 
                 q3.75% 
                 min 
                 max 
               
               
                   
                   
               
               
                   
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V1 
                 154.0188447 
                 11.28896522 
                 288.7462236 
               
               
                   
                 Galactosyllactose 
               
               
                   
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V2 
                 42.73797305 
                 13.62127529 
                 434.3117534 
               
               
                   
                 Galactosyllactose 
               
               
                   
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V3 
                 29.16381402 
                 7.291378016 
                 88.87964314 
               
               
                   
                 Galactosyllactose 
               
               
                   
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V4 
                 19.96432738 
                 6.838216821 
                 65.30491452 
               
               
                   
                 Galactosyllactose 
               
               
                   
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V5 
                 16.09249333 
                 6.514312709 
                 60.46113641 
               
               
                   
                 Galactosyllactose 
               
               
                   
                 6′ - 
                 mg/L 
                 UNIQUE 
                 V6 
                 13.69842265 
                 6.482990651 
                 146.8862427 
               
               
                   
                 Galactosyllactose 
               
               
                   
                 6′ - 
                 mg/L 
                 WITH 
                 V1 
                 168.6023267 
                 29.69176747 
                 258.227941 
               
               
                   
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
                 6′ - 
                 mg/L 
                 WITH 
                 V2 
                 44.46636476 
                 14.16036621 
                 98.52362497 
               
               
                   
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
                 6′- 
                 mg/L 
                 WITH 
                 V3 
                 29.31283111 
                 12.8653756 
                 85.11500318 
               
               
                   
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
                 6′ - 
                 mg/L 
                 WITH 
                 V4 
                 21.00367988 
                 6.668169255 
                 74.29089736 
               
               
                   
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
                 6′ - 
                 mg/L 
                 WITH 
                 V5 
                 17.61023478 
                 7.170486535 
                 55.96674008 
               
               
                   
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
                 6′ - 
                 mg/L 
                 WITH 
                 V6 
                 13.5930005 
                 6.800981073 
                 43.62702667 
               
               
                   
                 Galactosyllactose 
                   
                 SIBLINGS 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
               
               
               
             
               
                 TABLE VIII 
               
               
                   
               
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
               
               
               
               
            
               
                 BloodgroupA- 
                 mg/L 
                 UNIQUE 
                 V1 
                 62 
                 218.3100965 
                 199.91 
                 137.645 
                 96.501955 
                 339.8993275 
                 16.70496 
                 1198.809 
               
               
                 Tetrasaccharide 
               
               
                 BloodgroupA- 
                 mg/L 
                 UNIQUE 
                 V2 
                 74 
                 108.9752193 
                 87.04024 
                 73.80618 
                 49.86775 
                 153.6967025 
                 20.571 
                 406.27274 
               
               
                 Tetrasaccharide 
               
               
                 BloodgroupA- 
                 mg/L 
                 UNIQUE 
                 V3 
                 67 
                 101.1101424 
                 80.23 
                 74.20433 
                 44.016415 
                 113.37141 
                 18.04427 
                 388.594 
               
               
                 Tetrasaccharide 
               
               
                 BloodgroupA- 
                 mg/L 
                 UNIQUE 
                 V4 
                 59 
                 89.30594305 
                 70.23909 
                 58.313 
                 39.61766 
                 128.653605 
                 15.067 
                 338.339 
               
               
                 Tetrasaccharide 
               
               
                 BloodgroupA- 
                 mg/L 
                 UNIQUE 
                 V5 
                 53 
                 85.24264679 
                 64.13099 
                 62.96622 
                 35.832 
                 119.28 
                 17.93698 
                 269.711 
               
               
                 Tetrasaccharide 
               
               
                 BloodgroupA- 
                 mg/L 
                 UNIQUE 
                 V6 
                 48 
                 78.27483 
                 59.40327 
                 54.919015 
                 32.0109175 
                 113.239025 
                 15.08493 
                 214.554 
               
               
                 Tetrasaccharide 
               
               
                 BloodgroupA- 
                 mg/L 
                 WITH 
                 V1 
                 18 
                 224.7452844 
                 294.2047 
                 120.82463 
                 35.8747 
                 284.1568725 
                 16.98872 
                 993.8064 
               
               
                 Tetrasaccharide 
                   
                 SIBLINGS 
               
               
                 BloodgroupA- 
                 mg/L 
                 WITH 
                 V2 
                 18 
                 112.8672322 
                 117.5963 
                 75.509785 
                 48.8410875 
                 159.6912825 
                 15.208 
                 517.54089 
               
               
                 Tetrasaccharide 
                   
                 SIBLINGS 
               
               
                 BloodgroupA- 
                 mg/L 
                 WITH 
                 V3 
                 17 
                 123.0597341 
                 119.73 
                 95.024 
                 48.21369 
                 146.4399 
                 17.56554 
                 529.36 
               
               
                 Tetrasaccharide 
                   
                 SIBLINGS 
               
               
                 BloodgroupA- 
                 mg/L 
                 WITH 
                 V4 
                 17 
                 98.30965941 
                 83.89327 
                 77.95666 
                 57.209 
                 115.87755 
                 16.361 
                 376.29878 
               
               
                 Tetrasaccharide 
                   
                 SIBLINGS 
               
               
                 BloodgroupA- 
                 mg/L 
                 WITH 
                 V5 
                 15 
                 85.03220533 
                 69.04539 
                 67.01464 
                 40.436035 
                 107.519645 
                 19.39048 
                 294.134 
               
               
                 Tetrasaccharide 
                   
                 SIBLINGS 
               
               
                 BloodgroupA- 
                 mg/L 
                 WITH 
                 V6 
                 15 
                 70.211992 
                 56.09551 
                 51.54932 
                 35.436115 
                 88.07794 
                 21.94559 
                 246.20116 
               
               
                 Tetrasaccharide 
                   
                 SIBLINGS 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
               
             
               
                 TABLE IX 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
                 q1.25% 
               
               
                   
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 125 
                 166.66388 
                 100.4515893 
                 150.4463936 
                 98.1823431 
               
               
                 Hexaose-a 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 167 
                 282.797729 
                 161.029439 
                 247.1195693 
                 178.1422564 
               
               
                 Hexaose-a 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 144 
                 234.3063825 
                 147.4320416 
                 200.969341 
                 134.8599339 
               
               
                 Hexaose-a 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 118 
                 119.7067972 
                 96.5242152 
                 94.42787799 
                 52.25253247 
               
               
                 Hexaose-a 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 78 
                 98.51640533 
                 80.74105435 
                 72.88240189 
                 50.34510842 
               
               
                 Hexaose-a 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 58 
                 74.99587394 
                 51.32117734 
                 59.0456174 
                 42.88833806 
               
               
                 Hexaose-a 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 48 
                 148.346054 
                 81.70434542 
                 125.6285912 
                 94.72884661 
               
               
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 54 
                 261.9050265 
                 169.8874876 
                 231.2689262 
                 167.1776416 
               
               
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 51 
                 206.2678072 
                 144.2391624 
                 170.0672888 
                 110.5643357 
               
               
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 42 
                 120.121861 
                 100.4379777 
                 102.9007162 
                 56.84164741 
               
               
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 29 
                 97.3857747 
                 77.50806012 
                 71.724665 
                 59.86775755 
               
               
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 24 
                 76.45717107 
                 53.3864606 
                 65.56011998 
                 42.44962707 
               
               
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
               
               
            
               
                   
                 param 
                 unit 
                 DELITYP 
                 visit 
                 q3.75% 
                 min 
                 max 
               
               
                   
                   
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 208.71468 
                 37.68514348 
                 576.8930576 
               
               
                   
                 Hexaose-a 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 355.9677629 
                 33.08461539 
                 856.5390082 
               
               
                   
                 Hexaose-a 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 300.7426145 
                 39.43414695 
                 807.2575598 
               
               
                   
                 Hexaose-a 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 149.7452022 
                 33.55783812 
                 538.5644379 
               
               
                   
                 Hexaose-a 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 107.3617675 
                 33.27717717 
                 515.2208712 
               
               
                   
                 Hexaose-a 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 82.61864327 
                 33.45887998 
                 270.1883637 
               
               
                   
                 Hexaose-a 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 188.3725375 
                 33.04833329 
                 412.2467197 
               
               
                   
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 307.2992152 
                 41.111123 
                 1086.897955 
               
               
                   
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 253.965274 
                 42.14158522 
                 747.7649157 
               
               
                   
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 127.1671806 
                 34.70128067 
                 606.8605662 
               
               
                   
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 89.40771747 
                 33.00609983 
                 393.74294 
               
               
                   
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
                 Difucosyllacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 80.98116787 
                 33.43153153 
                 266.5264593 
               
               
                   
                 Hexaose-a 
                   
                 SIBLINGS 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE X 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 Mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 126 
                 1234.406373 
                 535.3466 
                 1180.198793 
               
               
                 Difucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 158 
                 1255.755653 
                 537.4429 
                 1192.294603 
               
               
                 Difucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 136 
                 1091.823028 
                 465.8642 
                 995.3117755 
               
               
                 Difucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 121 
                 848.0091077 
                 328.5295 
                 802.3504693 
               
               
                 Difucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 116 
                 710.6302809 
                 271.9261 
                 681.6194012 
               
               
                 Difucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 112 
                 634.9542185 
                 242.0775 
                 624.6350378 
               
               
                 Difucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 46 
                 1225.800254 
                 477.4247 
                 1169.458449 
               
               
                 Difucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 51 
                 1336.18374 
                 578.9028 
                 1348.485217 
               
               
                 Difucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 48 
                 1142.006226 
                 411.3581 
                 1114.291686 
               
               
                 Difucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 48 
                 825.7560502 
                 324.6839 
                 788.8361782 
               
               
                 Difucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 45 
                 739.1373379 
                 317.1183 
                 701.7743271 
               
               
                 Difucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 44 
                 578.2746578 
                 236.0452 
                 567.1716751 
               
               
                 Difucosylhexaose 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 849.3209761 
                 1495.918413 
                 11.58325101 
                 3346.954498 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 942.9128926 
                 1550.664139 
                 21.63261134 
                 3582.842867 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 821.2656843 
                 1344.286596 
                 10.97928599 
                 3501.352786 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 662.7755195 
                 1023.609883 
                 11.88700135 
                 1649.120744 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 541.1164043 
                 837.2962088 
                 103.063301 
                 1722.213919 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 471.4977544 
                 756.5617445 
                 111.6094256 
                 1485.00214 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 878.4507667 
                 1407.01817 
                 462.8830063 
                 2782.944079 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 1108.263831 
                 1576.688066 
                 12.29676207 
                 3031.332599 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 884.4304615 
                 1431.628644 
                 169.4986155 
                 1927.975206 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 631.0881501 
                 1035.617596 
                 74.64280102 
                 1917.735932 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 536.5070919 
                 882.8586901 
                 221.5440208 
                 1793.170075 
               
               
                   
                 Difucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 413.7894666 
                 739.2552491 
                 60.80285161 
                 1261.115824 
               
               
                   
                 Difucosylhexaose 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XI 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 Mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 139 
                 1953.405264 
                 895.9388 
                 1913.01447 
               
               
                 Fucosylpentaose-I 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 170 
                 1442.925271 
                 803.4876 
                 1369.9995 
               
               
                 Fucosylpentaose-I 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 149 
                 1080.473899 
                 640.1305 
                 933.32871 
               
               
                 Fucosylpentaose-I 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 133 
                 610.916764 
                 413.7517 
                 503.48892 
               
               
                 Fucosylpentaose-I 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 127 
                 470.6971887 
                 378.858 
                 376.267 
               
               
                 Fucosylpentaose-I 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 120 
                 388.8400843 
                 320.5121 
                 315.61007 
               
               
                 Fucosylpentaose-I 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 48 
                 1855.27654 
                 929.5294 
                 1704.10064 
               
               
                 Fucosylpentaose-I 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 54 
                 1391.449066 
                 788.47 
                 1172.082955 
               
               
                 Fucosylpentaose-I 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 52 
                 1044.350567 
                 593.307 
                 1006.408885 
               
               
                 Fucosylpentaose-I 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 50 
                 611.3230378 
                 451.9114 
                 562.65468 
               
               
                 Fucosylpentaose-I 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 48 
                 465.4264017 
                 359.7624 
                 380.606735 
               
               
                 Fucosylpentaose-I 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 45 
                 370.5976704 
                 304.8824 
                 293.10456 
               
               
                 Fucosylpentaose-I 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 1330.993545 
                 2520.18355 
                 27.4573 
                 4040.89442 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 781.2125 
                 2003.57863 
                 49.806 
                 3756.06871 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 573.47716 
                 1496.567 
                 28.866 
                 3306.848 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 283.029 
                 832.54689 
                 30.669 
                 1970.645 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 185.98 
                 617.2625 
                 30.394 
                 2062.94583 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 161.52072 
                 501.8237575 
                 27.51296 
                 1913.34495 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 1202.36626 
                 2417.197 
                 304.92323 
                 4311.24617 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 808.9399325 
                 1909.67225 
                 106.64425 
                 3571.06665 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 594.47375 
                 1446.30243 
                 77.48494 
                 2372.66273 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 285.0165 
                 781.02097 
                 36.497 
                 2068.53637 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 194.196455 
                 608.467225 
                 28.33995 
                 1643.26 
               
               
                   
                 Fucosylpentaose-I 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 153.08 
                 490.82 
                 43.879 
                 1622.958 
               
               
                   
                 Fucosylpentaose-I 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XII 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 145 
                 400.7219326 
                 518.1076 
                 192.6370398 
               
               
                 Fucosylpentaose-II 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 190 
                 544.0406658 
                 590.6689 
                 327.9102556 
               
               
                 Fucosylpentaose-II 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 165 
                 512.6256601 
                 521.4556 
                 320.0085042 
               
               
                 Fucosylpentaose-II 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 149 
                 461.1191582 
                 396.6782 
                 331.221059 
               
               
                 Fucosylpentaose-II 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 146 
                 420.9635001 
                 326.0906 
                 332.4659875 
               
               
                 Fucosylpentaose-II 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 138 
                 383.5239207 
                 276.9464 
                 293.9566112 
               
               
                 Fucosylpentaose-II 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 57 
                 474.9349989 
                 519.0685 
                 248.5627023 
               
               
                 Fucosylpentaose-II 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 70 
                 732.4415102 
                 712.1251 
                 428.9741842 
               
               
                 Fucosylpentaose-II 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 65 
                 642.6995951 
                 550.2848 
                 458.7201961 
               
               
                 Fucosylpentaose-II 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 64 
                 504.6233294 
                 416.96 
                 327.235739 
               
               
                 Fucosylpentaose-II 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 63 
                 461.6140679 
                 346.8066 
                 347.3093904 
               
               
                 Fucosylpentaose-II 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 61 
                 416.7272357 
                 300.1685 
                 326.0899963 
               
               
                 Fucosylpentaose-II 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 103.6228597 
                 323.2837721 
                 37.04755787 
                 2426.895823 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 182.5788937 
                 568.883943 
                 52.61106978 
                 2856.419409 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 184.3943767 
                 543.5270238 
                 53.24693544 
                 2488.674427 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 216.7351314 
                 562.6200229 
                 60.89431943 
                 2274.458362 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 200.0765447 
                 498.1834633 
                 53.63244898 
                 1714.785797 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 215.2587313 
                 449.8741781 
                 50.47498562 
                 1554.445901 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 124.7083137 
                 752.9452298 
                 38.05551582 
                 2146.380077 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 256.8757435 
                 1123.163255 
                 35.31341987 
                 3602.345161 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 240.4601421 
                 955.8715703 
                 88.28875496 
                 2623.432553 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 217.4510082 
                 639.6884927 
                 35.93501895 
                 2091.202327 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 231.9723537 
                 592.70929 
                 41.55891583 
                 1551.01748 
               
               
                   
                 Fucosylpentaose-II 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 214.4554786 
                 547.9185395 
                 44.29386143 
                 1488.541991 
               
               
                   
                 Fucosylpentaose-II 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
             
               
                   
                 TABLE XIII 
               
               
                   
                   
               
               
                   
                 Visit 
                 Estimate 
                 SE 
                 pvalue 
               
               
                   
                   
               
             
            
               
                   
                 V1 
                 1.276220 
                 1.132216 
                 0.04972522 
               
               
                   
                 V2 
                 1.350333 
                 1.130009 
                 0.01412686 
               
               
                   
                 V3 
                 1.337439 
                 1.131037 
                 0.01835975 
               
               
                   
                 V4 
                 1.211846 
                 1.131458 
                 0.12001568 
               
               
                   
                 V5 
                 1.191473 
                 1.131706 
                 0.15703207 
               
               
                   
                 V6 
                 1.147623 
                 1.132101 
                 0.26730874 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XIV 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 172 
                 440.7436567 
                 162.9639 
                 425.9454976 
               
               
                 Fucosylpentaose-III 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 214 
                 324.5042397 
                 155.5033 
                 307.6437027 
               
               
                 Fucosylpentaose-III 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 189 
                 313.7021468 
                 104.9384 
                 304.1777874 
               
               
                 Fucosylpentaose-III 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 173 
                 361.8867399 
                 117.8097 
                 352.9061564 
               
               
                 Fucosylpentaose-III 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 166 
                 355.1420518 
                 92.17895 
                 351.5526117 
               
               
                 Fucosylpentaose-III 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 158 
                 345.2252395 
                 93.83141 
                 337.6224468 
               
               
                 Fucosylpentaose-III 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 454.7211229 
                 173.722 
                 441.4279491 
               
               
                 Fucosylpentaose-III 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 75 
                 307.1033885 
                 86.78954 
                 304.261907 
               
               
                 Fucosylpentaose-III 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 71 
                 303.1843203 
                 78.66454 
                 293 
               
               
                 Fucosylpentaose-III 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 69 
                 348.8290902 
                 89.00072 
                 335.6039586 
               
               
                 Fucosylpentaose-III 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 67 
                 349.2434082 
                 93.08592 
                 345.6968971 
               
               
                 Fucosylpentaose-III 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 65 
                 323.8386581 
                 87.5295 
                 313.2473169 
               
               
                 Fucosylpentaose-III 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 326.9628144 
                 523.1014552 
                 117.4373288 
                 1151.993123 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 231.1082286 
                 388.025127 
                 79.94840168 
                 1751.020203 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 252.945844 
                 378.3723403 
                 97.1179054 
                 951.8773833 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 289.4064649 
                 414.3069494 
                 80.61587114 
                 1196.494936 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 281.5673515 
                 416.9454623 
                 166.2976602 
                 635.2849534 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 284.583471 
                 413.5865187 
                 138.1858699 
                 557.1545027 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 315.3262438 
                 541.2156412 
                 187.9371289 
                 890.3129598 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 246.1138864 
                 356.6258239 
                 87.25376406 
                 535.9760196 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 257.845359 
                 348.19862 
                 147.4060444 
                 563.392227 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 292.1571655 
                 405.4241552 
                 160.7903865 
                 543.5231017 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 278.5226687 
                 399.4384528 
                 126.2259899 
                 679.9790614 
               
               
                   
                 Fucosylpentaose-III 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 251.2469262 
                 383.3281509 
                 134.2613528 
                 567.5952348 
               
               
                   
                 Fucosylpentaose-III 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XV 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 112 
                 109.4842732 
                 107.2949 
                 48.9985 
               
               
                 Fucosylpentaose-V 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 175 
                 119.017012 
                 114.1155 
                 63.592 
               
               
                 Fucosylpentaose-V 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 155 
                 109.9870335 
                 101.3161 
                 61.36453 
               
               
                 Fucosylpentaose-V 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 144 
                 90.07381556 
                 72.94248 
                 58.491385 
               
               
                 Fucosylpentaose-V 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 135 
                 87.41945786 
                 70.56107 
                 57.784 
               
               
                 Fucosylpentaose-V 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 129 
                 76.81346488 
                 59.9275 
                 54.037 
               
               
                 Fucosylpentaose-V 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 46 
                 105.4405772 
                 91.79857 
                 57.939865 
               
               
                 Fucosylpentaose-V 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 68 
                 138.2168362 
                 125.1875 
                 84.83034 
               
               
                 Fucosylpentaose-V 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 65 
                 116.1223805 
                 95.20984 
                 75.977 
               
               
                 Fucosylpentaose-V 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 62 
                 91.59901602 
                 71.28318 
                 64.315775 
               
               
                 Fucosylpentaose-V 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 60 
                 79.1156255 
                 55.1115 
                 59.570785 
               
               
                 Fucosylpentaose-V 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 57 
                 73.00692544 
                 49.32591 
                 53.91017 
               
               
                 Fucosylpentaose-V 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 34.0883725 
                 176.98825 
                 24.098 
                 393.23939 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 42.54693 
                 172.5235 
                 24.15465 
                 495.7132 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 44.732165 
                 132.402505 
                 24.357 
                 427.275 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 41.540815 
                 120.62295 
                 24.31422 
                 412.37003 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 42.07251 
                 105.3295 
                 25.775 
                 360.11687 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 39.65592 
                 91.13553 
                 24.172 
                 325 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 36.8393575 
                 165.8721925 
                 24.184 
                 309.94073 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 49.35551 
                 224.66925 
                 25.57568 
                 515.4305 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 50.50503 
                 148.75439 
                 24.72194 
                 414.4 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 41.231875 
                 111.3105 
                 24.48 
                 360.47113 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 38.57574 
                 101.87361 
                 26.214 
                 243.549 
               
               
                   
                 Fucosylpentaose-V 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 38.03945 
                 97.496 
                 24.586 
                 232.777 
               
               
                   
                 Fucosylpentaose-V 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XVI 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 Mean 
                 Sd 
                 median 
               
               
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V1 
                 139 
                 69.65726947 
                 60.09699 
                 58.2952622 
               
               
                 (A) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V2 
                 205 
                 83.91299646 
                 44.09424 
                 72.90325819 
               
               
                 (A) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V3 
                 185 
                 71.67837167 
                 65.1411 
                 60.20319698 
               
               
                 (A) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V4 
                 154 
                 39.62268882 
                 18.54261 
                 34.7920641 
               
               
                 (A) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V5 
                 130 
                 30.26720457 
                 14.06454 
                 25.06469316 
               
               
                 (A) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V6 
                 107 
                 25.29538373 
                 9.751377 
                 21.98491257 
               
               
                 (A) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V1 
                 53 
                 69.44083736 
                 43.31972 
                 62.07164331 
               
               
                 (A) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V2 
                 74 
                 69.18762737 
                 31.78699 
                 60.56720348 
               
               
                 (A) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V3 
                 70 
                 62.49890449 
                 31.54116 
                 53.23506574 
               
               
                 (A) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V4 
                 63 
                 38.80460416 
                 20.67884 
                 34.70663487 
               
               
                 (A) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V5 
                 52 
                 33.10356562 
                 20.12426 
                 27.47100266 
               
               
                 (A) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V6 
                 51 
                 27.65797971 
                 11.52966 
                 24.0510901 
               
               
                 (A) 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N-hexaose 
                 38.17826996 
                 81.61276425 
                 18.36222878 
                 585.5330588 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 54.70601626 
                 109.8055481 
                 17.46965778 
                 255.4484418 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 43.02753693 
                 84.88738297 
                 16.19895295 
                 803.0893219 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 27.13590373 
                 46.62997118 
                 16.3928703 
                 112.8544379 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 20.88531751 
                 32.23440104 
                 16.0313543 
                 87.57130429 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 19.09685889 
                 27.93809463 
                 16.1076984 
                 68.12521273 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 46.00691942 
                 81.83799145 
                 16.96457388 
                 248.8007182 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 45.91588703 
                 82.53308105 
                 18.15175655 
                 169.5397907 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 41.84623544 
                 77.85927254 
                 17.24348703 
                 202.4682182 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 26.44253846 
                 44.29626051 
                 17.87155202 
                 139.2964627 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 22.26315853 
                 38.02796933 
                 16.26681559 
                 141.4095103 
               
               
                   
                 (A) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 20.30278179 
                 31.43146137 
                 16.26239728 
                 69.64415593 
               
               
                   
                 (A) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XVII 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 Visit 
                 N 
                 mean 
                 Sd 
                 median 
               
               
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V1 
                 134 
                 44.73295024 
                 34.32684 
                 33.28233637 
               
               
                 (B) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V2 
                 145 
                 43.11011358 
                 23.31967 
                 35.13295515 
               
               
                 (B) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V3 
                 133 
                 38.50327162 
                 21.12716 
                 32.35290783 
               
               
                 (B) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V4 
                 91 
                 33.26540708 
                 18.18317 
                 26.42650386 
               
               
                 (B) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V5 
                 59 
                 31.002883 
                 17.26554 
                 24.99800181 
               
               
                 (B) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 UNIQUE 
                 V6 
                 43 
                 27.72187917 
                 15.68486 
                 22.97054189 
               
               
                 (B) 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V1 
                 40 
                 41.5669005 
                 25.93896 
                 37.79728256 
               
               
                 (B) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V2 
                 36 
                 36.02515556 
                 16.13595 
                 30.58969053 
               
               
                 (B) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V3 
                 43 
                 34.28955775 
                 15.68827 
                 29.61018115 
               
               
                 (B) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V4 
                 29 
                 34.74970859 
                 13.55697 
                 31.13853753 
               
               
                 (B) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V5 
                 22 
                 28.51987306 
                 13.09366 
                 24.76177376 
               
               
                 (B) 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-hexaose 
                 mg/L 
                 WITH 
                 V6 
                 18 
                 28.88319141 
                 11.91772 
                 27.2941175 
               
               
                 (B) 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N-hexaose 
                 22.79344461 
                 53.48647374 
                 16.03905983 
                 220.7927384 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 27.90776152 
                 55.01012587 
                 16.31270557 
                 179.8286126 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 24.09883189 
                 45.67418316 
                 16.18714448 
                 131.3072962 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 19.69922881 
                 39.64344687 
                 16.41060296 
                 115.2056418 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 20.04178856 
                 33.98491795 
                 16.25904523 
                 105.2379451 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 18.6143141 
                 32.997549 
                 16.02806725 
                 99.84562336 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 24.8190705 
                 44.77882074 
                 16.60528968 
                 135.9994959 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 23.74009313 
                 46.45568574 
                 17.15234171 
                 84.77670516 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 23.11258219 
                 45.36245075 
                 16.5380127 
                 85.67908288 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 24.25250318 
                 43.6851328 
                 16.78734358 
                 76.04017383 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 18.2128352 
                 33.18017398 
                 16.04481676 
                 63.3735739 
               
               
                   
                 (B) 
                   
                   
                   
                   
               
               
                   
                 Lacto-N-hexaose 
                 20.37699792 
                 31.56511312 
                 16.83515397 
                 62.67504542 
               
               
                   
                 (B) 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XVIII 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 Visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 117 
                 111.9565166 
                 69.43106 
                 94.19356953 
               
               
                 Neodifucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 69 
                 63.29788709 
                 74.07394 
                 43.4578365 
               
               
                 Neodifucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 43 
                 57.46798821 
                 56.84744 
                 46.51065959 
               
               
                 Neodifucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 56 
                 55.60053979 
                 33.48608 
                 41.85130914 
               
               
                 Neodifucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 38 
                 54.58027932 
                 27.91287 
                 45.11014515 
               
               
                 Neodifucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 29 
                 64.33423068 
                 33.2452 
                 56.79116124 
               
               
                 Neodifucosylhexaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 46 
                 114.8602548 
                 82.49724 
                 80.63002523 
               
               
                 Neodifucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 19 
                 61.94019548 
                 45.97203 
                 45.4381543 
               
               
                 Neodifucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 15 
                 58.61383439 
                 39.13784 
                 54.97433839 
               
               
                 Neodifucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 19 
                 54.94306309 
                 22.66628 
                 48.37428768 
               
               
                 Neodifucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 18 
                 63.29320064 
                 32.14269 
                 53.26006424 
               
               
                 Neodifucosylhexaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 16 
                 58.9507447 
                 27.15766 
                 45.11453324 
               
               
                 Neodifucosylhexaose 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 59.70978875 
                 132.6595225 
                 29.32389989 
                 334.1268818 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 36.27672844 
                 68.9398609 
                 28.52752291 
                 612.4524307 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 32.51100498 
                 65.227353 
                 28.13123604 
                 398.4920634 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 34.6297264 
                 73.17992495 
                 28.10296364 
                 224.0399443 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 34.29570779 
                 63.77490019 
                 28.12614506 
                 141.5694861 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 35.66139704 
                 82.82903182 
                 28.64848952 
                 151.8818948 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 57.15833441 
                 153.8645579 
                 31.26569753 
                 350.3302321 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 35.75460343 
                 68.64819832 
                 29.95124997 
                 226.4465075 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 34.52825063 
                 66.41893776 
                 29.35106887 
                 187.0453095 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 40.02309424 
                 65.92514047 
                 28.49364998 
                 114.5744313 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 38.88978245 
                 79.91072693 
                 30.09514734 
                 140.8977209 
               
               
                   
                 Neodifucosylhexaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 39.24229995 
                 73.74432573 
                 32.02848488 
                 111.8547311 
               
               
                   
                 Neodifucosylhexaose 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XIX 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 50 
                 37.2525136 
                 16.38068 
                 33.67124 
               
               
                 Neofucosylpentaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 42 
                 29.88595143 
                 10.58937 
                 26.92816 
               
               
                 Neofucosylpentaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 40 
                 27.0749745 
                 9.243692 
                 23.80713 
               
               
                 Neofucosylpentaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 39 
                 30.60720744 
                 12.18325 
                 27.47212 
               
               
                 Neofucosylpentaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 31 
                 27.02506516 
                 6.34542 
                 25.69 
               
               
                 Neofucosylpentaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 23 
                 26.7532187 
                 7.552031 
                 24.091 
               
               
                 Neofucosylpentaose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 22 
                 35.40536864 
                 16.21748 
                 28.809265 
               
               
                 Neofucosylpentaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 16 
                 23.29734 
                 3.547994 
                 23.303695 
               
               
                 Neofucosylpentaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 11 
                 31.39661 
                 8.042168 
                 29.12481 
               
               
                 Neofucosylpentaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 12 
                 32.5542 
                 11.27676 
                 32.015775 
               
               
                 Neofucosylpentaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 11 
                 28.90526364 
                 10.61775 
                 25.04186 
               
               
                 Neofucosylpentaose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 9 
                 30.85879 
                 15.08243 
                 24.315 
               
               
                 Neofucosylpentaose 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 24.897 
                 41.76092 
                 19.73896 
                 92.18025 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 22.144305 
                 30.94125 
                 19.066 
                 59.578 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 21.4349075 
                 28.11675 
                 19.002 
                 53.52264 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 23.1454 
                 32.614335 
                 19.074 
                 82.885 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 22.51126 
                 28.6975 
                 19.037 
                 42.33404 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 20.97355 
                 29.805 
                 19.054 
                 48.112 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 26.059 
                 39.3506325 
                 20.14561 
                 84.56933 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 20.0572975 
                 25.788 
                 19.148 
                 30.76332 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 25.681345 
                 36.948515 
                 22.29 
                 46.081 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 23.654625 
                 35.353345 
                 22.437 
                 60.886 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 21.985635 
                 31.76967 
                 20.88696 
                 57.46976 
               
               
                   
                 Neofucosylpentaose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 20.677 
                 29.41272 
                 20.15457 
                 64.87128 
               
               
                   
                 Neofucosylpentaose 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XX 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 173 
                 307.7437813 
                 131.4372 
                 293.564 
               
               
                 Neotetraose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 213 
                 184.6575148 
                 100.9842 
                 173.50436 
               
               
                 Neotetraose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 187 
                 156.7024487 
                 82.56116 
                 146.70853 
               
               
                 Neotetraose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 168 
                 128.4345314 
                 83.21485 
                 107.724735 
               
               
                 Neotetraose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 158 
                 107.643116 
                 69.56969 
                 91.5955 
               
               
                 Neotetraose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 146 
                 98.41257377 
                 60.79302 
                 83.588435 
               
               
                 Neotetraose 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 304.2184716 
                 135.7726 
                 298.56318 
               
               
                 Neotetraose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 72 
                 154.2337461 
                 79.88463 
                 141.929085 
               
               
                 Neotetraose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 68 
                 144.1930094 
                 71.03133 
                 132.30853 
               
               
                 Neotetraose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 64 
                 126.1508667 
                 72.02305 
                 112.7993 
               
               
                 Neotetraose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 60 
                 107.7504913 
                 60.55004 
                 93.494 
               
               
                 Neotetraose 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 62 
                 96.30362226 
                 64.02489 
                 79.572765 
               
               
                 Neotetraose 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N- 
                 214.326 
                 389.59097 
                 47.75439 
                 699.27383 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 102.93676 
                 246.335 
                 25.73309 
                 597.13727 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 89.75674 
                 205.208 
                 26.64085 
                 424.58858 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 69.01625 
                 167.72524 
                 25.0473 
                 463.904 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 58.5215 
                 136.18827 
                 25.20536 
                 398.9075 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 52.0978825 
                 121.5750225 
                 25.22179 
                 298.346 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 219.730275 
                 373.411605 
                 64.40836 
                 695.668 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 96.1692475 
                 202.7789125 
                 32.626 
                 407.85 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 85.0371325 
                 200.53189 
                 31.748 
                 333.83 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 63.8390525 
                 179.90584 
                 25.575 
                 309.45348 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 62.7070925 
                 147.2496125 
                 31.655 
                 257.58145 
               
               
                   
                 Neotetraose 
                   
                   
                   
                   
               
               
                   
                 Lacto-N- 
                 47.7260275 
                 118.86836 
                 24.222 
                 277.71054 
               
               
                   
                 Neotetraose 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XXI 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 Sd 
                 median 
               
               
                   
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 UNIQUE 
                 V1 
                 173 
                 941.3105777 
                 869.2799 
                 668.05 
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 UNIQUE 
                 V2 
                 215 
                 1198.301294 
                 760.0552 
                 1066.41302 
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 UNIQUE 
                 V3 
                 190 
                 1013.002805 
                 642.5475 
                 860.403755 
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 UNIQUE 
                 V4 
                 174 
                 710.5043052 
                 451.1813 
                 624.944005 
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 UNIQUE 
                 V5 
                 167 
                 617.336245 
                 439.7945 
                 499.74 
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 UNIQUE 
                 V6 
                 159 
                 541.9378956 
                 396.6598 
                 460.991 
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 833.6287748 
                 579.2782 
                 791.530885 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 WITH 
                 V2 
                 75 
                 1255.222192 
                 591.7982 
                 1121.872 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 WITH 
                 V3 
                 71 
                 1000.002187 
                 425.3526 
                 947.02993 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 WITH 
                 V4 
                 68 
                 672.2036891 
                 307.2625 
                 647.973965 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 WITH 
                 V5 
                 67 
                 552.031873 
                 275.787 
                 479.85205 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lacto-N-Tetraose 
                 mg/L 
                 WITH 
                 V6 
                 63 
                 486.68108 
                 230.7009 
                 413.22725 
               
               
                   
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lacto-N-Tetraose 
                 389.089 
                 1247.059 
                 21.9529 
                 6714.31284 
               
               
                   
                 Lacto-N-Tetraose 
                 672.8633 
                 1443.05473 
                 125.055 
                 5360.81507 
               
               
                   
                 Lacto-N-Tetraose 
                 608.09198 
                 1259.128325 
                 119.024 
                 4011.92765 
               
               
                   
                 Lacto-N-Tetraose 
                 429.9219475 
                 815.3775375 
                 121.34189 
                 2894.36631 
               
               
                   
                 Lacto-N-Tetraose 
                 349.65877 
                 738.019535 
                 85.62092 
                 2932.955 
               
               
                   
                 Lacto-N-Tetraose 
                 300.9595 
                 640.624545 
                 101.62557 
                 3026.125 
               
               
                   
                 Lacto-N-Tetraose 
                 402.6631225 
                 1063.8035 
                 53.0063 
                 2461.67215 
               
               
                   
                 Lacto-N-Tetraose 
                 840.66133 
                 1577.087795 
                 161.241 
                 3144.60141 
               
               
                   
                 Lacto-N-Tetraose 
                 687.912885 
                 1289.76172 
                 147.76 
                 2176.884 
               
               
                   
                 Lacto-N-Tetraose 
                 424.3854625 
                 893.4178375 
                 125.368 
                 1648.35178 
               
               
                   
                 Lacto-N-Tetraose 
                 348.85265 
                 767.16389 
                 105.469 
                 1182.654 
               
               
                   
                 Lacto-N-Tetraose 
                 290.58641 
                 641.668435 
                 90.424 
                 971.2455 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XXII 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 sd 
                 median 
               
               
                   
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 UNIQUE 
                 V1 
                 138 
                 602.4483369 
                 532.6734 
                 420.450595 
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 UNIQUE 
                 V2 
                 168 
                 356.5481879 
                 418.4975 
                 226.1571229 
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 UNIQUE 
                 V3 
                 146 
                 276.3310824 
                 241.1256 
                 225.6436741 
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 UNIQUE 
                 V4 
                 132 
                 276.9074259 
                 155.7537 
                 235.3399016 
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 UNIQUE 
                 V5 
                 127 
                 262.4787906 
                 105.0967 
                 251.3244514 
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 UNIQUE 
                 V6 
                 122 
                 271.2121321 
                 118.9087 
                 251.5873565 
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 WITH 
                 V1 
                 48 
                 621.9134647 
                 632.5672 
                 446.7314741 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 WITH 
                 V2 
                 53 
                 324.4667808 
                 207.8789 
                 263.9484063 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 WITH 
                 V3 
                 52 
                 278.3663381 
                 202.4931 
                 229.1361688 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 WITH 
                 V4 
                 50 
                 288.8035427 
                 153.0885 
                 253.9644236 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 WITH 
                 V5 
                 48 
                 301.0084875 
                 180.131 
                 270.6795168 
               
               
                   
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Lactodifucosyllactose 
                 mg/L 
                 WITH 
                 V6 
                 46 
                 264.2267501 
                 143.745 
                 235.4535289 
               
               
                   
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Lactodifucosyllactose 
                 252.9317593 
                 740.5602398 
                 79.7125399 
                 2988.924526 
               
               
                   
                 Lactodifucosyllactose 
                 150.8612583 
                 382.6521639 
                 45.93249331 
                 2996.415918 
               
               
                   
                 Lactodifucosyllactose 
                 153.9841793 
                 302.7349125 
                 54.62821152 
                 1682.713383 
               
               
                   
                 Lactodifucosyllactose 
                 189.679307 
                 314.4043267 
                 47.58249391 
                 1108.712256 
               
               
                   
                 Lactodifucosyllactose 
                 194.6646696 
                 328.8925958 
                 54.61609346 
                 659.1978832 
               
               
                   
                 Lactodifucosyllactose 
                 197.9919531 
                 321.6287901 
                 51.57284974 
                 738.7201617 
               
               
                   
                 Lactodifucosyllactose 
                 226.8209576 
                 724.3811897 
                 50.73247046 
                 3297.275118 
               
               
                   
                 Lactodifucosyllactose 
                 179.9819135 
                 365.4112845 
                 85.89288249 
                 1006.992878 
               
               
                   
                 Lactodifucosyllactose 
                 148.2192763 
                 307.5226659 
                 49.85838655 
                 1155.744731 
               
               
                   
                 Lactodifucosyllactose 
                 185.9829117 
                 369.4069428 
                 48.51975875 
                 755.2157228 
               
               
                   
                 Lactodifucosyllactose 
                 159.9668191 
                 374.1570922 
                 46.25256273 
                 823.6766534 
               
               
                   
                 Lactodifucosyllactose 
                 166.4023321 
                 323.0766056 
                 51.0670661 
                 789.5430687 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XXIII 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 Sd 
                 median 
               
               
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 173 
                 120.4946707 
                 143.5211 
                 79.38738266 
               
               
                 Tetraose a 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 215 
                 120.320383 
                 174.7271 
                 67.4387729 
               
               
                 Tetraose a 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 190 
                 102.3350203 
                 162.4454 
                 52.40591398 
               
               
                 Tetraose a 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 174 
                 93.13419276 
                 147.0611 
                 43.84859549 
               
               
                 Tetraose a 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 167 
                 94.31415999 
                 147.0746 
                 49.15530418 
               
               
                 Tetraose a 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 159 
                 87.10861153 
                 104.4478 
                 53.16420322 
               
               
                 Tetraose a 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 131.8621806 
                 146.2816 
                 82.78296339 
               
               
                 Tetraose a 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 75 
                 156.1165221 
                 252.4851 
                 74.99139372 
               
               
                 Tetraose a 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 71 
                 120.6949772 
                 178.2896 
                 53.99733714 
               
               
                 Tetraose a 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 69 
                 107.0655282 
                 131.3995 
                 57.34829438 
               
               
                 Tetraose a 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 67 
                 116.0504387 
                 134.8519 
                 60.29079818 
               
               
                 Tetraose a 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 65 
                 104.5433758 
                 111.7794 
                 56.80548521 
               
               
                 Tetraose a 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Sialyllacto-N- 
                 47.18897931 
                 124.0410768 
                 0 
                 837.4945131 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 38.1468268 
                 107.9080843 
                 0 
                 1110.361583 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 27.44278706 
                 92.1621572 
                 0 
                 1121.248141 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 23.94542777 
                 89.71831532 
                 0 
                 1187.41635 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 26.76896814 
                 90.56411096 
                 0 
                 1133.515897 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 27.54758109 
                 103.3993222 
                 0 
                 613.7754102 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 51.7635368 
                 141.5571605 
                 0 
                 744.0657752 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 47.88950453 
                 132.3895992 
                 0 
                 1819.176794 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 35.99927184 
                 100.647476 
                 0 
                 1092.717349 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 33.00046274 
                 108.7613672 
                 0 
                 619.6592317 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 36.44169544 
                 133.7701709 
                 0 
                 566.2366019 
               
               
                   
                 Tetraose a 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 36.89919403 
                 147.2063842 
                 6.388328416 
                 492.4752761 
               
               
                   
                 Tetraose a 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
               
               
               
               
               
               
               
             
               
                 TABLE XXIV 
               
               
                   
               
             
            
               
                 param 
                 unit 
                 DELITYP 
                 visit 
                 N 
                 mean 
                 Sd 
                 median 
               
               
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V1 
                 170 
                 79.18246062 
                 42.28283 
                 68.72569099 
               
               
                 Tetraose b 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V2 
                 214 
                 78.58361782 
                 42.06355 
                 70.1670586 
               
               
                 Tetraose b 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V3 
                 189 
                 75.84894256 
                 38.80082 
                 67.84839427 
               
               
                 Tetraose b 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V4 
                 170 
                 64.81255413 
                 32.93704 
                 58.95748683 
               
               
                 Tetraose b 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V5 
                 162 
                 57.12345702 
                 30.98851 
                 50.59947014 
               
               
                 Tetraose b 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 UNIQUE 
                 V6 
                 155 
                 52.23655174 
                 29.0665 
                 44.63595583 
               
               
                 Tetraose b 
                   
                   
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V1 
                 64 
                 79.88850896 
                 33.7112 
                 72.77711539 
               
               
                 Tetraose b 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V2 
                 75 
                 82.99519595 
                 33.57784 
                 73.74143426 
               
               
                 Tetraose b 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V3 
                 71 
                 81.41914544 
                 34.10483 
                 78.12830055 
               
               
                 Tetraose b 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V4 
                 67 
                 63.55755653 
                 31.84949 
                 55.58607088 
               
               
                 Tetraose b 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V5 
                 66 
                 55.35928595 
                 31.36357 
                 46.55150531 
               
               
                 Tetraose b 
                   
                 SIBLINGS 
                   
                   
                   
                   
                   
               
               
                 Sialyllacto-N- 
                 mg/L 
                 WITH 
                 V6 
                 63 
                 45.79773144 
                 24.27547 
                 37.24666837 
               
               
                 Tetraose b 
                   
                 SIBLINGS 
               
               
                   
               
            
           
           
               
               
               
               
               
               
            
               
                   
                 param 
                 q1.25% 
                 q3.75% 
                 min 
                 max 
               
               
                   
               
               
                   
                 Sialyllacto-N- 
                 53.1564539 
                 90.75480198 
                 14.91737174 
                 275.7441984 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 48.80673735 
                 96.07204811 
                 19.62994206 
                 323.091464 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 47.47623501 
                 95.76248549 
                 18.2993516 
                 230.1699462 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 42.03248619 
                 77.48231245 
                 17.91900028 
                 203.3889653 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 34.20021019 
                 69.2258118 
                 15.77729891 
                 176.0331169 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 31.10428435 
                 63.33068783 
                 14.36759859 
                 161.4190188 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 59.09832119 
                 95.39076549 
                 36.70322691 
                 190.496288 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 63.00766627 
                 99.34148018 
                 16.07949427 
                 176.9251632 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 58.42526875 
                 97.31339889 
                 16.14913449 
                 192.4038113 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 40.10314569 
                 78.74882648 
                 17.09181849 
                 177.5190818 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 31.64970623 
                 67.96184529 
                 17.88112005 
                 159.9283979 
               
               
                   
                 Tetraose b 
                   
                   
                   
                   
               
               
                   
                 Sialyllacto-N- 
                 28.09090242 
                 56.62478649 
                 17.40002881 
                 118.0078565 
               
               
                   
                 Tetraose b 
               
               
                   
               
            
           
         
       
     
     Example 2 
     Table XXV sets out a human milk fortifier composition for in accordance with the invention. Said human milk fortifier may be for use to supplement the breast milk produced for an infant of up to 1 month of age by a primiparous mother. 
     
       
         
           
               
               
               
             
               
                 TABLE XV 
               
               
                   
               
               
                   
                 Nutrient 
                 Per 100 kcal 
               
               
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Energy (kcal) 
                 100 
               
               
                   
                 Lipid (g) 
                 9.76 
               
               
                   
                 DHA (mg) 
                 37.26 
               
               
                   
                 Linoleic acid (mg) 
                 1124.76 
               
               
                   
                 α-linolenic acid (mg) 
                 107.1 
               
               
                   
                 ARA (mg) 
                 47.68 
               
               
                   
                 ARA/DHA ratio 
                 1.28 
               
               
                   
                 Linoleic/α-linolenic ratio 
                 10.5 
               
               
                   
                 EPA (mg) 
                 4.06 
               
               
                   
                 EPA/DHA ratio 
                 0.11 
               
               
                   
                 MCT (g) 
                 1.4 
               
               
                   
                 Protein (g) 
                 0.7 
               
               
                   
                 Carbohydrate (g) 
                 2.3 
               
               
                   
                 Minerals and electrolytes 
                   
               
               
                   
                 Na (mg) 
                 71.25 
               
               
                   
                 K (mg) 
                 113.62 
               
               
                   
                 Cl (mg) 
                 100.12 
               
               
                   
                 Ca (mg) 
                 116.41 
               
               
                   
                 P (mg) 
                 69.27 
               
               
                   
                 Mg (mg) 
                 8.50 
               
               
                   
                 Mn (μg) 
                 7.40 
               
               
                   
                 Fe (mg) 
                 2.11 
               
               
                   
                 Cu (mg) 
                 0.10 
               
               
                   
                 Zn (mg) 
                 1.48 
               
               
                   
                 I (μg) 
                 33.76 
               
               
                   
                 Se (μg) 
                 6.75 
               
               
                   
                 F (μg) 
                 1.40 
               
               
                   
                 Cr (μg) 
                 0.88 
               
               
                   
                 Mo (μg) 
                 0.93 
               
               
                   
                 Vitamins and trace elements 
                   
               
               
                   
                 Vitamin A (μg) 
                 518.04 
               
               
                   
                 Vitamin D (μg) 
                 4.61 
               
               
                   
                 Vitamin E (mg) 
                 4.3 
               
               
                   
                 Vitamin K (μg) 
                 8.3 
               
               
                   
                 Vitamin C (mg) 
                 24.4 
               
               
                   
                 Vitamin B1 (mg) 
                 0.159 
               
               
                   
                 Vitamin B2 (mg) 
                 0.227 
               
               
                   
                 Niacin (mg) 
                 1.99 
               
               
                   
                 Vitamin B6 (mg) 
                 0.16 
               
               
                   
                 Folic acid (μg) 
                 50.17 
               
               
                   
                 Vitamin B12 (μg) 
                 0.26 
               
               
                   
                 Pantothenic acid (mg) 
                 1.08 
               
               
                   
                 Biotin (μg) 
                 4.70 
               
               
                   
                 Choline (mg) 
                 10.01 
               
               
                   
                 Inositol (mg) 
                 5.59 
               
               
                   
                 Taurine (mg) 
                 6.98 
               
               
                   
                 Carnitine (mg) 
                 4.89 
               
               
                   
                 3-Fucosyllactose (mg) 
                 13.5 
               
               
                   
                 Lacto-N-Fucosylpentaose II 
                 15 
               
               
                   
                 (mg) 
               
               
                   
               
            
           
         
       
     
     The composition according to the present invention may be formulated with many variations without departing from the scope of the invention as defined in the claims. 
     Example 3 
     Table XVI sets out an HMO human milk fortifier composition in accordance with the invention. Said human milk fortifier may be for use to supplement the breast milk produced for an infant of up to 1 month of age by a primiparous mother. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE XVI 
               
               
                   
                   
               
               
                   
                 HMO 
                 mg/L 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 3-Fucosyllactose 
                 90 
               
               
                   
                 Lacto-N-Fucosylpentaose II 
                 100 
               
               
                   
                 2′-Fucosyllactose 
                 100 
               
               
                   
                 Lactose 
                 ?? 
               
               
                   
                   
               
            
           
         
       
     
     Example 4 
     Table XVII sets out a human milk fortifier composition for in accordance with the invention. Said human milk fortifier may be for use to supplement the breast milk produced for an infant of up to 1 month of age by a multiparous mother. Said human milk fortifier composition may be comprised in a nutritional system with the human milk fortifier composition set out in example 2 wherein said composition of example 2 is specifally tailored for use to supplement the breast milk produced for an infant of up to 1 month of age by a primiparous mother. 
     
       
         
           
               
               
               
             
               
                   
                 TABLE XVII 
               
               
                   
                   
               
               
                   
                 Nutrient 
                 Per 100 kcal 
               
               
                   
                   
               
             
            
               
                   
               
            
           
           
               
               
               
            
               
                   
                 Energy (kcal) 
                 100 
               
               
                   
                 Lipid (g) 
                 9.76 
               
               
                   
                 DHA (mg) 
                 37.26 
               
               
                   
                 Linoleic acid (mg) 
                 1124.76 
               
               
                   
                 α-linolenic acid (mg) 
                 107.1 
               
               
                   
                 ARA (mg) 
                 47.68 
               
               
                   
                 ARA/DHA ratio 
                 1.28 
               
               
                   
                 Linoleic/α-linolenic ratio 
                 10.5 
               
               
                   
                 EPA (mg) 
                 4.06 
               
               
                   
                 EPA/DHA ratio 
                 0.11 
               
               
                   
                 MCT (g) 
                 1.4 
               
               
                   
                 Protein (g) 
                 0.7 
               
               
                   
                 Carbohydrate (g) 
                 2.3 
               
               
                   
                 Minerals and electrolytes 
                   
               
               
                   
                 Na (mg) 
                 71.25 
               
               
                   
                 K (mg) 
                 113.62 
               
               
                   
                 Cl (mg) 
                 100.12 
               
               
                   
                 Ca (mg) 
                 116.41 
               
               
                   
                 P (mg) 
                 69.27 
               
               
                   
                 Mg (mg) 
                 8.50 
               
               
                   
                 Mn (μg) 
                 7.40 
               
               
                   
                 Fe (mg) 
                 2.11 
               
               
                   
                 Cu (mg) 
                 0.10 
               
               
                   
                 Zn (mg) 
                 1.48 
               
               
                   
                 I (μg) 
                 33.76 
               
               
                   
                 Se (μg) 
                 6.75 
               
               
                   
                 F (μg) 
                 1.40 
               
               
                   
                 Cr (μg) 
                 0.88 
               
               
                   
                 Mo (μg) 
                 0.93 
               
               
                   
                 Vitamins and trace elements 
                   
               
               
                   
                 Vitamin A (μg) 
                 518.04 
               
               
                   
                 Vitamin D (μg) 
                 4.61 
               
               
                   
                 Vitamin E (mg) 
                 4.3 
               
               
                   
                 Vitamin K (μg) 
                 8.3 
               
               
                   
                 Vitamin C (mg) 
                 24.4 
               
               
                   
                 Vitamin B1 (mg) 
                 0.159 
               
               
                   
                 Vitamin B2 (mg) 
                 0.227 
               
               
                   
                 Niacin (mg) 
                 1.99 
               
               
                   
                 Vitamin B6 (mg) 
                 0.16 
               
               
                   
                 Folic acid (μg) 
                 50.17 
               
               
                   
                 Vitamin B12 (μg) 
                 0.26 
               
               
                   
                 Pantothenic acid (mg) 
                 1.08 
               
               
                   
                 Biotin (μg) 
                 4.70 
               
               
                   
                 Choline (mg) 
                 10.01 
               
               
                   
                 Inositol (mg) 
                 5.59 
               
               
                   
                 Taurine (mg) 
                 6.98 
               
               
                   
                 Carnitine (mg) 
                 4.89 
               
               
                   
                   
               
            
           
         
       
     
     The composition according to the present invention may be formulated with many variations without departing from the scope of the invention as defined in the claims.