Patent Publication Number: US-2016222460-A1

Title: Molecular diagnostic test for oesophageal cancer

Description:
FIELD OF THE INVENTION 
     The present invention relates to a molecular diagnostic test useful for predicting responsiveness of oesophageal cancers to particular treatments that includes the use of a DNA damage repair deficiency subtype. The invention includes the generation and use of various classifiers derived from identification of this subtype in oesophageal cancer patients (in particular oesophageal adenocarcinoma (OAC) patients), such as use of a 44-gene classification model that is used to identify this DNA damage repair deficiency molecular subtype. One application is the stratification of response to, and selection of patients for Oesophageal cancer therapeutic drug classes, including DNA damage causing agents and DNA repair targeted therapies. The present invention provides a test that can guide conventional therapy selection as well as selecting patient groups for enrichment strategies during clinical trial evaluation of novel therapeutics. DNA repair deficient subtypes can be identified, for example, from fresh/frozen (FF) or formalin fixed paraffin embedded (FFPE) patient samples. 
     BACKGROUND 
     The pharmaceutical industry continuously pursues new drug treatment options that are more effective, more specific or have fewer adverse side effects than currently administered drugs. Drug therapy alternatives are constantly being developed because genetic variability within the human population results in substantial differences in the effectiveness of many drugs. Therefore, although a wide variety of drug therapy options are currently available; more therapies are always needed in the event that a patient fails to respond. 
     Traditionally, the treatment paradigm used by physicians has been to prescribe a first-line drug therapy that results in the highest success rate possible for treating a disease. Alternative drug therapies are then prescribed if the first is ineffective. This paradigm is clearly not the best treatment method for certain diseases. For example, in diseases such as cancer, the first treatment is often the most important and offers the best opportunity for successful therapy, so there exists a heightened need to choose an initial drug that will be the most effective against that particular patient&#39;s disease. 
     Oesophageal cancer (cancer of the food pipe) is a highly aggressive disease, ranking among the 10 most common cancers in the world. It has become more common in the past 30 years in the UK. It is the 9th most common cancer in adults, with around 8,500 cases diagnosed each year in the UK (CRUK statistics). Oesophageal cancer is about twice as common in men as in women. Adenocarcinoma means a cancer that has started in glandular cells. In oesophageal cancer, these are the cells that make mucus in the lining of the oesophagus. The number of adenocarcinomas has increased in the last 20 years. They now make up just over a half of all oesophageal cancers diagnosed, with Squamous cell carcinoma accounting for the remaining occurrences. Adenocarcinomas are found mainly in the lower third of the oesophagus. 
     Treatment of early stage Oesophageal adenocarcinoma (OAC) is generally surgery. Surgical therapy is the only treatment that has repeatedly been shown to provide prolonged survival, albeit in only approximately 20% of cases (Müller J M et al. Br J Surg (1990) 77:845). The results of surgical resection can be excellent. Five year survival rate is over 80% when tumours are confined to the mucosa and between 50% and 80% when the submucosa is involved. Bonavina L. Br J Surg (1995) 82:98; Hölscher A H, et al. Br J Surg (1997) 84:1470. 
     The advent of microarrays and molecular genomics has the potential for a significant impact on the diagnostic capability and prognostic classification of disease, which may aid in the prediction of the response of an individual patient to a defined therapeutic regimen. Microarrays provide for the analysis of large amounts of genetic information, thereby providing a genetic fingerprint of an individual. There is much enthusiasm that this technology will ultimately provide the necessary tools for custom-made drug treatment regimens. 
     Currently, healthcare professionals have few mechanisms to help them identify cancer patients who will benefit from chemotherapeutic agents. Identification of the optimal first-line drug has been difficult because methods are not available for accurately predicting which drug treatment would be the most effective for a particular cancer&#39;s physiology. This deficiency results in relatively poor single agent response rates and increased cancer morbidity and death. Furthermore, patients often needlessly undergo ineffective, toxic drug therapy. 
     Molecular markers have been used to select appropriate treatments, for example, in breast cancer. Breast tumors that do not express the estrogen and progesterone hormone receptors as well as the HER2 growth factor receptor, called “triple negative”, appear to be responsive to PARP-1 inhibitor therapy (Linn, S. C., and Van&#39;t Veer, L., J. Eur J Cancer 45 Suppl 1, 11-26 (2009); O&#39;Shaughnessy, J., et al. N Engl J Med 364, 205-214 (2011). Recent studies indicate that the triple negative status of a breast tumor may indicate responsiveness to combination therapy including PARP-1 inhibitors, but may not be sufficient to indicate responsiveness to individual PARP-1 inhibitors (O&#39;Shaughnessy et al., 2011). 
     Furthermore, there have been other studies that have attempted to identify gene classifiers associated with molecular subtypes to indicate responsiveness of chemotherapeutic agents (Farmer et al. Nat Med 15, 68-74 (2009); Konstantinopoulos, P. A., et al., J Clin Oncol 28, 3555-3561 (2010)). 
     WO 2012/037378 describes a 44-gene DNA microarray assay, the DNA damage repair deficient (DDRD) assay. This assay identifies a molecular subgroup of cancers that have lost the DNA damage response FA/BRCA pathway, resulting in sensitivity to DNA damaging chemotherapeutic agents (Kennedy &amp; D&#39;Andrea Journal of Clinical Oncology (2006) 24:3799, Turner et al Nature Reviews Cancer (2004) 4:814). 
     In breast cancer the DDRD assay has been shown to predict response to neoadjuvant DNA-damaging chemotherapy (5-fluorouracil, anthracycline and cyclophosphamide) in 203 breast cancer patients (odd ratio 4.01) (95% CI:1.69-9.54). In a cohort of 191 early breast cancer patients treated with adjuvant 5-fluorouracil, epirubicin and cyclophosphamide treatment, the assay predicted 5-year relapse free survival with a hazard ratio of 0.37 (95% CI:0.15-0.88). 
     SUMMARY OF THE INVENTION 
     In many instances of OAC, surgery follows neoadjuvant chemotherapy, usually a combination of cisplatin and fluorouracil. This therapy is used to reduce the size of the tumour. However it is known that not all patients respond to this form of therapy and there is, as yet, no test to identify those OAC patients that will benefit from neoadjuvant chemotherapy. Consequently many patients are undergoing chemotherapy, and the associated side effects and reduction in quality of life, without a therapeutic benefit. The DNA damage response pathway has been shown to be important in the progression of oesophageal cancer (He et al (2013) Carginogeneisis 34:139; Motoori et al International Journal of Oncology (2010) 37:1113). Consequently there is a great clinical need for a test to stratify OAC patients with respect to neoadjuvant chemotherapy in advance of surgery. 
     The present invention is based upon application of methods that identify deficiencies in DNA damage repair to determine which patients will benefit from certain therapies, such as platinum-based therapy in order to treat oesophageal cancer, specifically OAC. The invention is directed to methods of using a collection of gene product markers expressed in oesophageal cancer such that when some or all of the transcripts are over or under-expressed, they identify a subtype of oesophageal cancer that has a deficiency in DNA damage repair. The invention also provides methods for indicating responsiveness or resistance to DNA-damaging therapeutic agents. In different aspects, this gene or gene product list may form the basis of a single parameter or a multiparametric predictive test that could be delivered using methods known in the art such as microarray, Q-PCR, immunohistochemistry, ELISA or other technologies that can quantify mRNA or protein expression. 
     Thus, according to one aspect of the invention there is provided a method of predicting responsiveness of an individual having oesophageal cancer such as (in particular) oesophageal adenocarcinoma (OAC) to treatment with a DNA-damaging therapeutic agent comprising: 
     a. measuring expression levels of one or more biomarkers/genes in a test sample obtained from the individual, wherein the one or more biomarkers are selected from Table 1A, 1B, 2A, 2B, 3 and/or 4 such as one or more biomarkers/genes selected from the group consisting of CXCL10, MX1, IDO1, IF144L, CD2, GBP5, PRAME, ITGAL, LRP4, and APOL3; 
     b. deriving a test score that captures the expression levels; 
     c. providing a threshold score comprising information correlating the test score and responsiveness; 
     d. and comparing the test score to the threshold score; wherein responsiveness is predicted when the test score exceeds the threshold score and/or wherein a lack of responsiveness is predicted when the test score does not exceed the threshold score. 
     The methods may be performed as a method for selecting a suitable treatment for an individual or as a test and treat method. Thus, in certain embodiments if the test score exceeds the threshold score (responsiveness is predicted) the individual is treated with the DNA-damaging therapeutic agent. Similarly, if the test score does not exceed the threshold score (responsiveness is not predicted) the individual is not treated with the DNA-damaging therapeutic agent. In those circumstances, alternative treatments may be contemplated. For OAC, the alternative treatments may comprise administration of a mitotic inhibitor, such as a  vinca  alkaloid or a taxane. Example  vinca  alkaloids include vinorelbine. Example taxanes include paclitaxel or docetaxel. Alternatively, the treatment may exclude chemotherapy altogether. The methods can, in some embodiments, also involve the subsequent treatment of the individual identified as responsive. Corresponding kits are also contemplated. The method is typically performed in vitro. The method is, therefore, performed using an isolated, or pre-isolated, sample. In some embodiments, the methods may encompass the step of obtaining a test sample from the individual. In certain embodiments, the method comprises measuring an expression level of at least 10 of the biomarkers from any of Tables 1 to 4 in the test sample. More specifically, the method may comprise measuring the expression level of at least 10 and up to all 44 different biomarkers listed in Table 2B in some embodiments. In certain embodiments, expression levels are measured using primers or probes which bind to at least one of the target sequences set forth in Tables 1A, 1B or 3 (as SEQ ID NO: 1-24, SEQ ID NO: 25-50 or SEQ ID NO: 51-230). 
     In some embodiments, the method further comprises measuring an expression level of one or more biomarkers in the test sample, wherein the one or more biomarkers are selected from the group consisting of CDR1, FYB, TSPAN7, RAC2, KLHDC7B, GRB14, AC138128.1, KIF26A, CD274, CD109, ETV7, MFAP5, OLFM4, PI15, FOSB, FAM19A5, NLRC5, PRICKLE1, EGR1, CLDN10, ADAMTS4, SP140L, ANXA1, RSAD2, ESR1, IKZF3, OR2I1P, EGFR, NAT1, LATS2, CYP2B6, PTPRC, PPP1R1A, and AL137218.1. In certain embodiments, the test score captures the expression levels of all of the biomarkers. In some embodiments, responsiveness may be predicted when the test score exceeds a threshold score at a value of between approximately 0.1 and 0.5 such as 0.1, 0.2, 0.3, 0.4 or 0.5. for example approximately 0.3681. 
     The oesophageal cancer is typically oesophageal adenocarcinoma (OAC) and may be early stage. Alternatively, the OAC may be late stage or metastatic disease. The treatment for which responsiveness is predicted is typically neoadjuvant treatment. However, it may comprise adjuvant treatment additionally or alternatively. 
     The invention described herein is not limited to any one DNA-damaging therapeutic agent; it can be used to identify responders and non-responders to any of a range of DNA-damaging therapeutic agents, for example those that directly or indirectly affect DNA damage and/or DNA damage repair. In some embodiments, the DNA-damaging therapeutic agent comprises one or more substances selected from the group consisting of: a DNA damaging agent, a DNA repair targeted therapy, an inhibitor of DNA damage signalling, an inhibitor of DNA damage induced cell cycle arrest, a histone deacetylase inhibitor, a heat shock protein inhibitor and an inhibitor of DNA synthesis. More specifically, the DNA-damaging therapeutic agent may be selected from one or more of a platinum-containing agent, a nucleoside analogue such as gemcitabine or 5-fluorouracil or a prodrug thereof such as capecitabine, an anthracycline such as epirubicin or doxorubicin, an alkylating agent such as cyclophosphamide, an ionising radiation or a combination of radiation and chemotherapy (chemoradiation). In particular embodiments, the DNA-damaging therapeutic agent comprises a platinum-containing agent, such as a platinum based agent selected from cisplatin, carboplatin and oxaliplatin. For example, it is shown experimentally herein that the methods of the invention can predict responsiveness/act as a prognostic indicator of OAC patients who are more likely to benefit from platinum based therapies. The methods may predict responsiveness to treatment with the DNA-damaging therapeutic agent together with a further therapy or drug. Thus, the methods may predict responsiveness to a combination therapy. Thus, in some embodiments, the further drug is a mitotic inhibitor. The mitotic inhibitor may be a  vinca  alkaloid or a taxane. In specific embodiments, the  vinca  alkaloid is vinorelbine. In other embodiments, the taxane is paclitaxel or docetaxel. In certain embodiments, responders to the following (combination) treatments are identified: cisplatin/carboplatin and 5-fluorouracil (CF), cisplatin/carboplatin and capecitabine (CX), epirubicin/doxorubicin, cisplatin/carboplatin and fluorouracil (ECF), epirubicin/doxorubicin, oxaliplatin and capecitabine (EOX), and combinations with paclitaxel, irinotecan and vinorelbine, radiation or chemoradiation with or without taxanes, in treatment of oespohageal cancer. 
     The present invention relates to prediction of response to drugs (DNA-damaging therapeutic agents) using different classifications of response, such as overall survival, progression free survival, disease free survival, radiological response, as defined by RECIST, complete response, partial response, stable disease and serological markers such as, but not limited to, PSA, CEA, CA125, CA15-3 and CA19-9. In other embodiments of this invention it can be used to evaluate endoscopic ultrasound, CT, spiral CT, FDG-PET, pathologic, histological response in oesophageal cancer (OAC) treated with DNA damaging combination therapies, alone or in the context of standard treatment. 
     The present invention relies upon a DNA damage response deficiency (DDRD) molecular subtype, originally identified in breast and ovarian cancer (WO2012/037378; incorporated herein by reference). This molecular subtype can, in some embodiments, be detected by the use of two different gene classifiers—one being 40 genes in length and one being 44 genes in length. The DDRD classifier was first defined by a classifier consisting of 53 probesets on the Almac Breast Disease Specific Array (DSA™). So as to validate the functional relevance of this classifier in the context of its ability to predict response to DNA-damaging containing chemotherapy regimens, the classifier needed to be re-defined at a gene level. This facilitated evaluation of the DDRD classifier using microarray data from independent datasets that were profiled on microarray platforms other than the Almac Breast DSA®. In order to facilitate defining the classifier at a gene level, the genes to which the Almac Breast DSA® probesets map needed to be defined. This involved the utilization of publicly available genome browser databases such as Ensembl and NCBI Reference Sequence. The 44-gene DDRD classifier model supersedes that of the 40-gene DDRD classifier model. The results presented herein demonstrate that the 44 and 40 gene classifier models and related classifier models derived from the markers disclosed herein are effective and significant predictors of response to chemotherapy regimens that contain DNA damaging therapeutics in the context of OAC. 
     The identification of the DDRD subtype using classifier models based upon genes taken from Tables 1A and 1B (or Tables 3 and 4), such as by both the 40-gene classifier model (see Tables 2A and 2B) and the 44-gene classifier model, can be used to predict response to, and select patients for, standard OAC therapeutic drug classes, including DNA damage causing agents and DNA repair targeted therapies. 
     In another aspect, the present invention relates to kits for conventional diagnostic uses listed above such as nucleic acid amplification, including PCR and all variants thereof such as real-time and end point methods and qPCR, Next generation Sequencing (NGS), microarray, and immunoassays such as immunohistochemistry, ELISA, Western blot and the like. Such kits include appropriate reagents and directions to assay the expression of the genes or gene products and quantify mRNA or protein expression. The kits may include suitable primers and/or probes to detect the expression levels of at least one of the genes in Table 1A and/or 1B and/or 2A and/or 2B and/or 3 and/or 4. The kits may contain primers and/or probes that bind to target sequences set forth in Tables 1A, 1B and/or 3. The target sequences may comprise, consist essentially of or consisting of SEQ ID NO: 1-24, SEQ ID NO: 25-50 or SEQ ID NO: 51-230. Where expression is determined at the protein level the kit may contain binding reagents specific for the proteins of interest. The binding reagents may comprise antibodies to include all fragments and derivatives thereof. In the context of the various embodiments of the present invention the term “antibody” includes all immunoglobulins or immunoglobulin-like molecules with specific binding affinity for the relevant protein (including by way of example and without limitation, IgA, IgD, IgE, IgG and IgM, combinations thereof, and similar molecules produced during an immune response in any vertebrate, for example, in mammals such as humans, goats, rabbits and mice). Specific immunoglobulins useful in the various embodiments of the invention include IgG isotypes. The antibodies useful in the various embodiments of the invention may be monoclonal or polyclonal in origin, but are typically monoclonal antibodies. Antibodies may be human antibodies, non-human antibodies, or humanized versions of non-human antibodies, or chimeric antibodies. Various techniques for antibody humanization are well established and any suitable technique may be employed. The term “antibody” also refers to a polypeptide ligand comprising at least a light chain or heavy chain immunoglobulin variable region which specifically recognizes and binds an epitope of an antigen, and it extends to all antibody derivatives and fragments that retain the ability to specifically bind to the relevant protein. These derivatives and fragments may include Fab fragments, F(ab′) 2  fragments, Fv fragments, single chain antibodies, single domain antibodies, Fc fragments etc. The term antibody encompasses antibodies comprised of both heavy and light chains, but also heavy chain (only) antibodies (which may be derived from various species of cartilaginous fish or camelids). In specific embodiments, the antibodies may be engineered so as to be specific for more than protein, for example bi-specific to permit binding to two different target proteins as identified herein (see Tables 1 to 4). 
     In some embodiments, the kits may also contain the specific DNA-damaging therapeutic agent to be administered in the event that the test predicts responsiveness. This agent may be provided in a form, such as a dosage form, that is tailored to OAC treatment specifically. The kit may be provided with suitable instructions for administration according to OAC treatment regimens. 
     The invention also provides methods for identifying DNA damage response-deficient (DDRD) human OAC tumors. It is likely that this invention can be used to identify patients that are sensitive to and respond, or are resistant to and do not respond, to DNA-damaging therapeutic agents, such as drugs that damage DNA directly, damage DNA indirectly or inhibit normal DNA damage signaling and/or repair processes. 
     The invention also relates to guiding conventional treatment of patients. The invention also relates to selecting patients for clinical trials where novel DNA-damaging therapeutic agents, such as drugs of the classes that directly or indirectly affect DNA damage and/or DNA damage repair are to be tested specifically for treatment of OAC. 
     The present invention and methods accommodate the use of archived formalin fixed paraffin-embedded (FFPE) biopsy material, including fine needle aspiration (FNA) as well as fresh/frozen (FF) tissue, for assay of all transcripts in the invention, and are therefore compatible with the most widely available type of biopsy material. The expression level may be determined using RNA obtained from FFPE tissue, fresh frozen tissue or fresh tissue that has been stored in solutions such as RNAlater®. 
     DETAILED DESCRIPTION OF THE INVENTION 
     Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods, devices, and materials similar or equivalent to those described herein can be used in the practice or testing of the invention, the preferred methods, devices and materials are now described. 
     All publications, published patent documents, and patent applications cited in this application are indicative of the level of skill in the art(s) to which the application pertains. All publications, published patent documents, and patent applications cited herein are hereby incorporated by reference to the same extent as though each individual publication, published patent document, or patent application was specifically and individually indicated as being incorporated by reference. 
     The articles “a” and “an” are used herein to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article. By way of example, “an element” means one element or more than one element, unless explicitly indicated to the contrary. 
     A major goal of current research efforts in cancer is to increase the efficacy of perioperative systemic therapy in patients by incorporating molecular parameters into clinical therapeutic decisions. Pharmacogenetics/genomics is the study of genetic/genomic factors involved in an individual&#39;s response to a foreign compound or drug. Agents or modulators which have a stimulatory or inhibitory effect on expression of a marker of the invention can be administered to individuals to treat (prophylactically or therapeutically) oesophageal cancer in a patient. It is ideal to also consider the pharmacogenomics of the individual in conjunction with such treatment. Differences in metabolism of therapeutics may possibly lead to severe toxicity or therapeutic failure by altering the relationship between dose and blood concentration of the pharmacologically active drug. Thus, understanding the pharmacogenomics of an individual permits the selection of effective agents (e.g., drugs) for prophylactic or therapeutic treatments. Such pharmacogenomics can further be used to determine appropriate dosages and therapeutic regimens. Accordingly, the level of expression of a marker of the invention in an individual can be determined to thereby select appropriate agent(s) for therapeutic or prophylactic treatment of the individual. 
     The invention is directed to the application of a collection of gene or gene product markers (hereinafter referred to as “biomarkers”) expressed in certain oesophageal cancer tissue for predicting responsiveness to treatment using DNA-damaging therapeutic agents. In different aspects, this biomarker list may form the basis of a single parameter or multiparametric predictive test that could be delivered using methods known in the art such as microarray, Q-PCR, NGS, immunohistochemistry, ELISA or other technologies that can quantify mRNA or protein expression. 
     The present invention also relates to kits and methods that are useful for prognosis following cytotoxic chemotherapy or selection of specific treatments for oesophageal cancer (particularly OAC). Methods are provided such that when some or all of the transcripts are over or under-expressed, the expression profile indicates responsiveness or resistance to DNA-damaging therapeutic agents. These kits and methods employ gene or gene product markers that are differentially expressed in tumors of patients with oesophageal adenocarcinoma (OAC). In one embodiment of the invention, the expression profiles of these biomarkers are correlated with clinical outcome (response or survival) in archival tissue samples under a statistical method or a correlation model to create a database or model correlating expression profile with responsiveness to one or more DNA-damaging therapeutic agents. The predictive model may then be used to predict the responsiveness in a patient whose responsiveness to the DNA-damaging therapeutic agent(s) is unknown. In many other embodiments, a patient population can be divided into at least two classes based on patients&#39; clinical outcome, prognosis, or responsiveness to DNA-damaging therapeutic agents, and the biomarkers are substantially correlated with a class distinction between these classes of patients. The biological pathways described herein have been shown to be predictive of responsiveness to treatment of OAC using DNA-damaging therapeutic agents. 
     Predictive Marker Panels/Expression Classifiers 
     A collection of biomarkers as a genetic classifier expressed in oesophageal cancer/OAC tissue is provided that is useful in determining responsiveness or resistance to therapeutic agents, such as DNA-damaging therapeutic agents, used to treat oesophageal cancer/OAC. Such a collection may be termed a “marker panel”, “expression classifier”, or “classifier”. The collection is shown in Table 1. This collection was derived from an original collection of biomarkers as shown in Tables 1A and 1B (see WO 2012/037378) which were then mapped to an OAC-relevant platform (see the Example herein). Application of a classifier such as the 44 gene classifier shown in Table 2B permits responsiveness of OAC patients to DNA-damaging therapeutic agents to be determined. The invention may involve determining expression levels of any one or more of these genes or target sequences. 
     The biomarkers useful in the present methods are thus identified in Tables 1 to 4. These biomarkers are identified as having predictive value to determine a patient (having OAC) response to a therapeutic agent, or lack thereof. Their expression correlates with the response to an agent, and more specifically, a DNA-damaging therapeutic agent. By examining the expression of a collection of the identified biomarkers in an oesophageal tumor, in particular an adenocarcinoma or squamous cell carcinoma, it is possible to determine which therapeutic agent or combination of agents will be most likely to reduce the growth rate of the cancer, and in some embodiments, OAC cells. By examining a collection of identified transcript gene or gene product markers, it is also possible to determine which therapeutic agent or combination of agents will be the least likely to reduce the growth rate of the cancer. By examining the expression of a collection of biomarkers, it is therefore possible to eliminate ineffective or inappropriate therapeutic agents. Importantly, in certain embodiments, these determinations can be made on a patient-by-patient basis or on an agent-by-agent basis. Thus, one can determine whether or not a particular therapeutic regimen is likely to benefit a particular patient or type of patient, and/or whether a particular regimen should be continued. 
     
       
         
           
               
             
               
                 TABLE 1A 
               
             
            
               
                   
               
               
                 Original list of genes tested in breast cancer and mapped to OAC (via Xcel array) 
               
            
           
           
               
               
               
               
            
               
                 Sense genes (166) 
                   
                   
                   
               
            
           
           
               
               
               
               
            
               
                   
                 EntrezGene 
                 Antisense of known genes (24) 
                 SEQ ID 
               
            
           
           
               
               
               
               
               
            
               
                 Gene Symbol 
                 ID 
                 Almac Gene ID 
                 Almac Gene symbol 
                 NO: 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 ABCA12 
                 26154 
                   
                 N/A 
                   
               
               
                 ALDH3B2 
                 222 
                   
                 N/A 
                   
               
               
                 APOBEC3G 
                 60489 
                   
                 N/A 
                   
               
               
                 APOC1 
                 341 
                   
                 N/A 
                   
               
               
                 APOL6 
                 80830 
                   
                 N/A 
                   
               
               
                 ARHGAP9 
                 64333 
                   
                 N/A 
                   
               
               
                 BAMBI 
                 25805 
                   
                 N/A 
                   
               
               
                 BIK 
                 638 
                   
                 N/A 
                   
               
               
                 BIRC3 
                 330 
                 AS1_BIRC3 
                 Hs127799.0C7n9_at 
                 1 
               
               
                 BTN3A3 
                 10384 
                   
                 N/A 
                   
               
               
                 C12orf48 
                 55010 
                   
                 N/A 
                   
               
               
                 C17orf28 
                 283987 
                   
                 N/A 
                   
               
               
                 C1orf162 
                 128346 
                   
                 N/A 
                   
               
               
                 C1orf64 
                 149563 
                   
                 N/A 
                   
               
               
                 C1QA 
                 712 
                   
                 N/A 
                   
               
               
                 C21orf70 
                 85395 
                   
                 N/A 
                   
               
               
                 C22orf32 
                 91689 
                   
                 N/A 
                   
               
               
                 C6orf211 
                 79624 
                   
                 N/A 
                   
               
               
                 CACNG4 
                 27092 
                   
                 N/A 
                   
               
               
                 CCDC69 
                 26112 
                   
                 N/A 
                   
               
               
                 CCL5 
                 6352 
                   
                 N/A 
                   
               
               
                 CCNB2 
                 9133 
                   
                 N/A 
                   
               
               
                 CCND1 
                 595 
                   
                 N/A 
                   
               
               
                 CCR7 
                 1236 
                   
                 N/A 
                   
               
               
                 CD163 
                 9332 
                   
                 N/A 
                   
               
               
                 CD2 
                 914 
                   
                 N/A 
                   
               
               
                 CD22 
                 933 
                   
                 N/A 
                   
               
               
                 CD24 
                 100133941 
                   
                 N/A 
                   
               
               
                 CD274 
                 29126 
                   
                 N/A 
                   
               
               
                 CD3D 
                 915 
                   
                 N/A 
                   
               
               
                 CD3E 
                 916 
                   
                 N/A 
                   
               
               
                 CD52 
                 1043 
                   
                 N/A 
                   
               
               
                 CD53 
                 963 
                   
                 N/A 
                   
               
               
                 CD79A 
                 973 
                   
                 N/A 
                   
               
               
                 CDH1 
                 999 
                   
                 N/A 
                   
               
               
                 CDKN3 
                 1033 
                   
                 N/A 
                   
               
               
                 CECR1 
                 51816 
                   
                 N/A 
                   
               
               
                 CHEK1 
                 1111 
                   
                 N/A 
                   
               
               
                 CKMT1B 
                 1159 
                   
                 N/A 
                   
               
               
                 CMPK2 
                 129607 
                   
                 N/A 
                   
               
               
                 CNTNAP2 
                 26047 
                   
                 N/A 
                   
               
               
                 COX16 
                 51241 
                   
                 N/A 
                   
               
               
                 CRIP1 
                 1396 
                   
                 N/A 
                   
               
               
                 CXCL10 
                 3627 
                   
                 N/A 
                   
               
               
                 CXCL9 
                 4283 
                   
                 N/A 
                   
               
               
                 CYBB 
                 1536 
                   
                 N/A 
                   
               
               
                 CYP2B6 
                 1555 
                   
                 N/A 
                   
               
               
                 DDX58 
                 23586 
                   
                 N/A 
                   
               
               
                 DDX60L 
                 91351 
                   
                 N/A 
                   
               
               
                 ERBB2 
                 2064 
                   
                 N/A 
                   
               
               
                 ETV7 
                 51513 
                   
                 N/A 
                   
               
               
                 FADS2 
                 9415 
                   
                 N/A 
                   
               
               
                 FAM26F 
                 441168 
                   
                 N/A 
                   
               
               
                 FAM46C 
                 54855 
                   
                 N/A 
                   
               
               
                 FASN 
                 2194 
                   
                 N/A 
                   
               
               
                 FBP1 
                 2203 
                   
                 N/A 
                   
               
               
                 FBXO2 
                 26232 
                   
                 N/A 
                   
               
               
                 FKBP4 
                 2288 
                   
                 N/A 
                   
               
               
                 FLJ40330 
                 645784 
                   
                 N/A 
                   
               
               
                 FYB 
                 2533 
                   
                 N/A 
                   
               
               
                 GBP1 
                 2633 
                   
                 N/A 
                   
               
               
                 GBP4 
                 115361 
                   
                 N/A 
                   
               
               
                 GBP5 
                 115362 
                 AS1_GBP5 
                 BRMX.5143C1n2_at 
                 2 
               
               
                 GIMAP4 
                 55303 
                   
                 N/A 
                   
               
               
                 GLRX 
                 2745 
                   
                 N/A 
                   
               
               
                 GLUL 
                 2752 
                   
                 N/A 
                   
               
               
                 GVIN1 
                 387751 
                   
                 N/A 
                   
               
               
                 H2AFJ 
                 55766 
                   
                 N/A 
                   
               
               
                 HGD 
                 3081 
                   
                 N/A 
                   
               
               
                 HIST1H2BK 
                 85236 
                   
                 N/A 
                   
               
               
                 HIST3H2A 
                 92815 
                   
                 N/A 
                   
               
               
                 HLA-DOA 
                 3111 
                   
                 N/A 
                   
               
               
                 HLA-DPB1 
                 3115 
                   
                 N/A 
                   
               
               
                 HMGB2 
                 3148 
                   
                 N/A 
                   
               
               
                 HMGB3 
                 3149 
                   
                 N/A 
                   
               
               
                 HSP90AA1 
                 3320 
                   
                 N/A 
                   
               
               
                 IDO1 
                 3620 
                   
                 N/A 
                   
               
               
                 IFI27 
                 3429 
                   
                 N/A 
                   
               
               
                 IFI44 
                 10561 
                   
                 N/A 
                   
               
               
                 IFI44L 
                 10964  
                 AS1_IF144L 
                 BRSA.1606C1n4_at 
                 3 
               
               
                 IFI6 
                 2537 
                   
                 N/A 
                   
               
               
                 IFIH1 
                 64135 
                   
                 N/A 
                   
               
               
                 IGJ 
                 3512  
                 AS1_IGJ 
                 BRIH.1231C2n2_at 
                 4 
               
               
                 IKZF1 
                 10320 
                   
                 N/A 
                   
               
               
                 IL10RA 
                 3587 
                   
                 N/A 
                   
               
               
                 IL2RG 
                 3561 
                   
                 N/A 
                   
               
               
                 IL7R 
                 3575 
                   
                 N/A 
                   
               
               
                 IMPAD1 
                 54928 
                   
                 N/A 
                   
               
               
                 IQGAP3 
                 128239 
                 AS1_IQGAP3 
                 BRAD.30779_s_at 
                 5 
               
               
                 IRF1 
                 3659 
                   
                 N/A 
                   
               
               
                 ISG15 
                 9636 
                   
                 N/A 
                   
               
               
                 ITGAL 
                 3683 
                   
                 N/A 
                   
               
               
                 KIAA1467 
                 57613 
                   
                 N/A 
                   
               
               
                 KIF20A 
                 10112 
                   
                 N/A 
                   
               
               
                 KITLG 
                 4254 
                   
                 N/A 
                   
               
               
                 KLRK1 
                 22914 
                   
                 N/A 
                   
               
               
                 KRT19 
                 3880 
                   
                 N/A 
                   
               
               
                 LAIR1 
                 3903 
                   
                 N/A 
                   
               
               
                 LCP1 
                 3936 
                   
                 N/A 
                   
               
               
                 LOC100289702 
                 100289702 
                   
                 N/A 
                   
               
               
                 LOC100294459 
                 100294459 
                 AS1_LOC100294459 
                 BRSA.396C1n2_at 
                 6 
               
               
                 LOC150519 
                 150519 
                   
                 N/A 
                   
               
               
                 LOC439949 
                 439949 
                   
                 N/A 
                   
               
               
                 LYZ 
                 4069 
                   
                 N/A 
                   
               
               
                 MAL2 
                 114569 
                   
                 N/A 
                   
               
               
                 MGC29506 
                 51237 
                   
                 N/A 
                   
               
               
                 MIAT 
                 440823 
                   
                 N/A 
                   
               
               
                 MS4A1 
                 931 
                   
                 N/A 
                   
               
               
                 MX1 
                 4599  
                 AS1_MX1 
                 BRMX.2948C3n7_at 
                 7 
               
               
                 NAPSB 
                 256236 
                   
                 N/A 
                   
               
               
                 NCKAP1L 
                 3071 
                   
                 N/A 
                   
               
               
                 NEK2 
                 4751 
                   
                 N/A 
                   
               
               
                 NLRC3 
                 197358 
                   
                 N/A 
                   
               
               
                 NLRC5 
                 84166 
                   
                 N/A 
                   
               
               
                 NPNT 
                 255743 
                   
                 N/A 
                   
               
               
                 NQO1 
                 1728 
                   
                 N/A 
                   
               
               
                 OAS2 
                 4939 
                   
                 N/A 
                   
               
               
                 OAS3 
                 4940 
                   
                 N/A 
                   
               
               
                 PAQR4 
                 124222 
                   
                 N/A 
                   
               
               
                 PARP14 
                 54625 
                   
                 N/A 
                   
               
               
                 PARP9 
                 83666 
                   
                 N/A 
                   
               
               
                 PIK3CG 
                 5294 
                   
                 N/A 
                   
               
               
                 PIM2 
                 11040 
                   
                 N/A 
                   
               
               
                 PLEK 
                 5341 
                   
                 N/A 
                   
               
               
                 POU2AF1 
                 5450 
                   
                 N/A 
                   
               
               
                 PP14571 
                 100130449 
                   
                 N/A 
                   
               
               
                 PPP2R2C 
                 5522 
                   
                 N/A 
                   
               
               
                 PSMB9 
                 5698 
                   
                 N/A 
                   
               
               
                 PTPRC 
                 5788 
                   
                 N/A 
                   
               
               
                 RAC2 
                 5880 
                   
                 N/A 
                   
               
               
                 RAMP1 
                 10267 
                   
                 N/A 
                   
               
               
                 RARA 
                 5914 
                   
                 N/A 
                   
               
               
                 RASSF7 
                 8045 
                   
                 N/A 
                   
               
               
                 RSAD2 
                 91543 
                   
                 N/A 
                   
               
               
                 RTP4 
                 64108 
                   
                 N/A 
                   
               
               
                 SAMD9 
                 54809 
                   
                 N/A 
                   
               
               
                 SAMD9L 
                 219285 
                   
                 N/A 
                   
               
               
                 SASH3 
                 54440 
                   
                 N/A 
                   
               
               
                 SCD 
                 6319 
                   
                 N/A 
                   
               
               
                 SELL 
                 6402 
                   
                 N/A 
                   
               
               
                 SIX1 
                 6495 
                 AS1_SIX1 
                 Hs539969.0C4n3_at 
                 8 
               
               
                 SLAMF7 
                 57823 
                   
                 N/A 
                   
               
               
                 SLC12A2 
                 6558 
                   
                 N/A 
                   
               
               
                 SLC9A3R1 
                 9368 
                 AS1_SLC9A3R1 
                 Hs396783.3C1n4_at 
                 9 
               
               
                 SPOCK2 
                 9806 
                   
                 N/A 
                   
               
               
                 SQLE 
                 6713 
                   
                 N/A 
                   
               
               
                 ST20 
                 400410 
                   
                 N/A 
                   
               
               
                 ST6GALNAC2 
                 10610 
                   
                 N/A 
                   
               
               
                 STAT1 
                 6772 
                 AS1_STAT1 
                 BRMX.13670C1n2_at 
                 10 
               
               
                 STRA13 
                 201254 
                   
                 N/A 
                   
               
               
                 SUSD4 
                 55061 
                   
                 N/A 
                   
               
               
                 SYT12 
                 91683 
                   
                 N/A 
                   
               
               
                 TAP1 
                 6890 
                   
                 N/A 
                   
               
               
                 TBC1D10C 
                 374403 
                   
                 N/A 
                   
               
               
                 TNFRSF13B 
                 23495 
                   
                 N/A 
                   
               
               
                 TNFSF10 
                 8743 
                   
                 N/A 
                   
               
               
                 TOB1 
                 10140 
                 AS1_TOB1 
                 BRAD.30243_at 
                 11 
               
               
                 TOM1L1 
                 10040 
                   
                 N/A 
                   
               
               
                 TRIM22 
                 10346 
                   
                 N/A 
                   
               
               
                 UBD 
                 10537 
                 AS1_UBD 
                 BRMX.941C2n2_at 
                 12 
               
               
                 UBE2T 
                 29089 
                   
                 N/A 
                   
               
               
                 UCK2 
                 7371 
                   
                 N/A 
                   
               
               
                 USP18 
                 11274 
                   
                 N/A 
                   
               
               
                 VNN2 
                 8875 
                   
                 N/A 
                   
               
               
                 XAF1 
                 54739 
                   
                 N/A 
                   
               
               
                 ZWINT 
                 11130 
                   
                 N/A 
                   
               
               
                   
                   
                 AS1_C1QC 
                 BRMX.4154C1n3_s_at  
                 13 
               
               
                   
                   
                 AS1_C2orf14 
                 BRAD.39498_at 
                 14 
               
               
                   
                   
                 AS1_EPSTI1 
                 BRAD.34868_s_at 
                 15 
               
               
                   
                   
                 AS1_GALNT6 
                 5505575.0C1n42_at 
                 16 
               
               
                   
                   
                 AS1_HIST1H4H 
                 BREM.1442_at 
                 17 
               
               
                   
                   
                 AS1_HIST2H4B 
                 BRHP.827_s_at 
                 18 
               
               
                   
                   
                 AS2_HIST2H4B 
                 BRRS.18322_s_at 
                 19 
               
               
                   
                   
                 AS3_HIST2H4B 
                 BRRS.18792_s_at 
                 20 
               
               
                   
                   
                 AS1_KIAA1244 
                 Hs632609.0C1n37_at 
                 21 
               
               
                   
                   
                 AS1_LOC100287927 
                 Hs449575.0C1n22_at  
                 22 
               
               
                   
                   
                 AS1_LOC100291682 
                 BRAD.18827_s_at 
                 23 
               
               
                   
                   
                 AS1_LOC100293679 
                 BREM.2466_s_at 
                 24 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 1B 
               
             
            
               
                   
               
               
                 Original list of genes tested in breast cancer  
               
               
                 and mapped to OAC (via Xcel array) 
               
               
                 Novel genes 
               
            
           
           
               
               
               
            
               
                   
                 Gene symbol 
                 SEQ ID NO: 
               
               
                   
                   
               
               
                   
                 BRAD.2605_at 
                 25 
               
               
                   
                 BRAD.33618_at 
                 26 
               
               
                   
                 BRAD.36579_s_at 
                 27 
               
               
                   
                 BRAD1_5440961_s_at 
                 28 
               
               
                   
                 BRAD1_66786229_s_at 
                 29 
               
               
                   
                 BREM.2104_at 
                 30 
               
               
                   
                 BRAG_AK097020.1_at 
                 31 
               
               
                   
                 BRAD.20415_at 
                 32 
               
               
                   
                 BRAD.29668_at 
                 33 
               
               
                   
                 BRAD.30228_at 
                 34 
               
               
                   
                 BRAD.34830_at 
                 35 
               
               
                   
                 BRAD.37011_s_at 
                 36 
               
               
                   
                 BRAD.37762_at 
                 37 
               
               
                   
                 BRAD.40217_at 
                 38 
               
               
                   
                 BRAD1_4307876_at 
                 39 
               
               
                   
                 BREM.2505_at 
                 40 
               
               
                   
                 Hs149363.0CB4n5_s_at 
                 41 
               
               
                   
                 Hs172587.9C1n9_at 
                 42 
               
               
                   
                 Hs271955.16C1n9_at 
                 43 
               
               
                   
                 Hs368433.18C1n6_at 
                 44 
               
               
                   
                 Hs435736.0C1n27_s_at 
                 45 
               
               
                   
                 Hs493096.15C1n6_at 
                 46 
               
               
                   
                 Hs493096.2C1n15_s_at 
                 47 
               
               
                   
                 Hs592929.0CB2n8_at 
                 48 
               
               
                   
                 Hs79953.0C1n23_at 
                 49 
               
               
                   
                 BRMX.2377C1n3_at 
                 50 
               
               
                   
                   
               
            
           
         
       
     
     All or a portion of the biomarkers recited in Tables 1A and/or 1B and/or 3 may be used in a predictive biomarker panel. For example, biomarker panels selected from the biomarkers in Tables 1A and/or 1B and/or 3 can be generated using the methods provided herein and can comprise between one, and all of the biomarkers set forth in Tables 1A and/or 1B and/or 3 and each and every combination in between (e.g., four selected biomarkers, 16 selected biomarkers, 74 selected biomarkers, etc.). In some embodiments, the predictive biomarker set comprises at least 5, 10, 20, 40, 60, 100, 150, 200, or 300 or more biomarkers. In other embodiments, the predictive biomarker set comprises no more than 5, 10, 20, 40, 60, 100, 150, 200, 300, 400, 500, 600 or 700 biomarkers. In some embodiments, the predictive biomarker set includes a plurality of biomarkers listed in Tables 1A and/or 1B and/or 3. In some embodiments the predictive biomarker set includes at least about 1%, about 5%, about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, about 96%, about 97%, about 98%, or about 99% of the biomarkers listed in Tables 1A and/or 1B and/or 3. Selected predictive biomarker sets can be assembled from the predictive biomarkers provided using methods described herein and analogous methods known in the art. In one embodiment, the biomarker panel contains all 50 biomarkers in Table 1 or all biomarkers listed in Table 3. In another embodiment, the biomarker panel corresponds to the 40 or 44 gene panel described in Tables 2A and 2B. 
     Predictive biomarker sets may be defined in combination with corresponding scalar weights on the real scale with varying magnitude, which are further combined through linear or non-linear, algebraic, trigonometric or correlative means into a single scalar value via an algebraic, statistical learning, Bayesian, regression, or similar algorithms which together with a mathematically derived decision function on the scalar value provide a predictive model by which expression profiles from samples may be resolved into discrete classes of responder or non-responder, resistant or non-resistant, to a specified drug or drug class. Such predictive models, including biomarker membership, are developed by learning weights and the decision threshold, optimized for sensitivity, specificity, negative and positive predictive values, hazard ratio or any combination thereof, under cross-validation, bootstrapping or similar sampling techniques, from a set of representative expression profiles from historical patient samples with known drug response and/or resistance or with known molecular subtype (i.e. DDRD) classification. 
     In one embodiment, the biomarkers are used to form a weighted sum of their signals, where individual weights can be positive or negative. The resulting sum (“decisive function”) is compared with a pre-determined reference point or value. The comparison with the reference point or value may be used to diagnose, or predict a clinical condition or outcome. 
     As described above, one of ordinary skill in the art will appreciate that the biomarkers included in the classifier or classifiers provided in Tables 1A and/or 1B and/or 3 will carry unequal weights in a classifier for responsiveness or resistance to a therapeutic agent. Therefore, while as few as one sequence may be used to diagnose or predict an outcome such as responsiveness to therapeutic agent, the specificity and sensitivity or diagnosis or prediction accuracy may increase using more sequences. 
     As used herein, the term “weight” refers to the relative importance of an item in a statistical calculation. The weight of each biomarker in a gene expression classifier may be determined on a data set of patient samples using analytical methods known in the art. Gene specific bias values may also be applied. Gene specific bias may be required to mean centre each gene in the classifier relative to a training data set, as would be understood by one skilled in the art. Specific bias values are presented in Table 4 and may be applied according to the methods and kits of the invention. 
     In one embodiment the biomarker panel is directed to the 40 biomarkers detailed in Table 2A with corresponding ranks and weights detailed in the table or alternative rankings and weightings, depending, for example, on the disease setting. In another embodiment, the biomarker panel is directed to the 44 biomarkers detailed in Table 2B with corresponding ranks and weights detailed in the table or alternative rankings and weightings, depending, for example, on the disease setting. Tables 2A and 2B rank the biomarkers in order of decreasing weight in the classifier, defined as the rank of the average weight in the compound decision score function measured under cross-validation. In another embodiment, the biomarker panel is directed to the 44 biomarkers detailed in Table 4 with corresponding ranks, weights and bias detailed in the table or alternative rankings, weightings and bias, depending, for example, on the disease setting. 
     
       
         
           
               
             
               
                 TABLE 2A 
               
             
            
               
                   
               
               
                 Gene IDs and EntrezGene IDs for 40-gene DDRD classifier model 
               
               
                 with associated ranking and weightings 
               
               
                 DDRD classifier 40 gene model 
               
            
           
           
               
               
               
               
            
               
                 Rank 
                 Genes Symbol 
                 EntrezGene ID 
                 Weights 
               
               
                   
               
            
           
           
               
               
               
               
            
               
                  1 
                 GBP5 
                 115362 
                 0.022389581 
               
               
                  2 
                 CXCL10 
                 3627 
                 0.021941734 
               
               
                  3 
                 IDO1 
                 3620 
                 0.020991115 
               
               
                  4 
                 MX1 
                 4599 
                 0.020098675 
               
               
                  5 
                 IFI44L 
                 10964 
                 0.018204957 
               
               
                  6 
                 CD2 
                 914 
                 0.018080661 
               
               
                  7 
                 PRAME 
                 23532 
                 0.016850837 
               
               
                  8 
                 ITGAL 
                 3683 
                 0.016783359 
               
               
                  9 
                 LRP4 
                 4038 
                 −0.015129969 
               
               
                 10 
                 SP140L 
                 93349 
                 0.014646025 
               
               
                 11 
                 APOL3 
                 80833 
                 0.014407174 
               
               
                 12 
                 FOSB 
                 2354 
                 −0.014310521 
               
               
                 13 
                 CDR1 
                 1038 
                 −0.014209848 
               
               
                 14 
                 RSAD2 
                 91543 
                 0.014177132 
               
               
                 15 
                 TSPAN7 
                 7102 
                 −0.014111562 
               
               
                 16 
                 RAC2 
                 5880 
                 0.014093627 
               
               
                 17 
                 FYB 
                 2533 
                 0.01400475 
               
               
                 18 
                 KLHDC7B 
                 113730 
                 0.013298413 
               
               
                 19 
                 GRB14 
                 2888 
                 0.013031204 
               
               
                 20 
                 KIF26A 
                 26153 
                 −0.012942351 
               
               
                 21 
                 CD274 
                 29126 
                 0.012651964 
               
               
                 22 
                 CD109 
                 135228 
                 −0.012239425 
               
               
                 23 
                 ETV7 
                 51513 
                 0.011787297 
               
               
                 24 
                 MFAP5 
                 8076 
                 −0.011480443 
               
               
                 25 
                 OLFM4 
                 10562 
                 −0.011130113 
               
               
                 26  
                 PI15 
                 51050 
                 −0.010904326 
               
               
                 27  
                 FAM19A5  
                 25817 
                 −0.010500936 
               
               
                 28  
                 NLRC5 
                 84166 
                 0.009593449 
               
               
                 29  
                 EGR1 
                 1958 
                 −0.008947963 
               
               
                 30  
                 ANXA1 
                 301 
                 −0.008373991 
               
               
                 31  
                 CLDN10 
                 9071 
                 −0.008165127 
               
               
                 32  
                 ADAMTS4 
                 9507 
                 −0.008109892 
               
               
                 33  
                 ESR1 
                 2099 
                 0.007524594 
               
               
                 34  
                 PTPRC 
                 5788 
                 0.007258669 
               
               
                 35  
                 EGFR 
                 1956 
                 −0.007176203 
               
               
                 36  
                 NAT1 
                 9 
                 0.006165534 
               
               
                 37  
                 LATS2 
                 26524 
                 −0.005951091 
               
               
                 38  
                 CYP2B6 
                 1555 
                 0.005838391 
               
               
                 39 
                 PPP1R1A 
                 5502 
                 −0.003898835 
               
               
                 40  
                 TERF1P1  
                 348567 
                 0.002706847 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 2B 
               
             
            
               
                   
               
               
                 Gene IDs and EntrezGene IDs for 44-gene DDRD classifier  
               
               
                 model with associated ranking and weightings 
               
               
                 DDRD Classifier-44 Gene Model (NA: genomic sequence) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Rank 
                 Gene symbol 
                 EntrezGene ID 
                 Weight 
               
               
                   
                   
               
            
           
           
               
               
               
               
               
            
               
                   
                  1 
                 CXCL10 
                 3627 
                 0.023 
               
               
                   
                  2 
                 MX1 
                 4599 
                 0.0226 
               
               
                   
                  3 
                 IDO1 
                 3620 
                 0.0221 
               
               
                   
                  4 
                 IFI44L 
                 10964 
                 0.0191 
               
               
                   
                  5 
                 CD2 
                 914 
                 0.019 
               
               
                   
                  6 
                 GBP5 
                 115362 
                 0.0181 
               
               
                   
                  7 
                 PRAME 
                 23532 
                 0.0177 
               
               
                   
                  8 
                 ITGAL 
                 3683 
                 0.0176 
               
               
                   
                  9 
                 LRP4 
                 4038 
                 −0.0159 
               
               
                   
                 10 
                 APOL3 
                 80833 
                 0.0151 
               
               
                   
                 11 
                 CDR1 
                 1038 
                 −0.0149 
               
               
                   
                 12 
                 FYB 
                 2533 
                 −0.0149 
               
               
                   
                 13 
                 TSPAN7 
                 7102 
                 0.0148 
               
               
                   
                 14 
                 RAC2 
                 5880 
                 −0.0148 
               
               
                   
                 15 
                 KLHDC7B 
                 113730 
                 0.014 
               
               
                   
                 16 
                 GRB14 
                 2888 
                 0.0137 
               
               
                   
                 17 
                 AC138128.1 
                 N/A 
                 −0.0136 
               
               
                   
                 18 
                 KIF26A 
                 26153 
                 −0.0136 
               
               
                   
                 19 
                 CD274 
                 29126 
                 0.0133 
               
               
                   
                 20 
                 CD109 
                 135228 
                 −0.0129 
               
               
                   
                 21 
                 ETV7 
                 51513 
                 0.0124 
               
               
                   
                 22 
                 MFAP5 
                 8076 
                 −0.0121 
               
               
                   
                 23 
                 OLFM4 
                 10562 
                 −0.0117 
               
               
                   
                 24 
                 PI15 
                 51050 
                 −0.0115 
               
               
                   
                 25 
                 FOSB 
                 2354 
                 −0.0111 
               
               
                   
                 26 
                 FAM19A5 
                 25817 
                 0.0101 
               
               
                   
                 27 
                 NLRC5 
                 84166 
                 −0.011 
               
               
                   
                 28 
                 PRICKLE1 
                 144165 
                 −0.0089 
               
               
                   
                 29 
                 EGR1 
                 1958 
                 −0.0086 
               
               
                   
                 30 
                 CLDN10 
                 9071 
                 −0.0086 
               
               
                   
                 31 
                 ADAMTS4 
                 9507 
                 −0.0085 
               
               
                   
                 32 
                 SP140L 
                 93349 
                 0.0084 
               
               
                   
                 33 
                 ANXA1 
                 301 
                 −0.0082 
               
               
                   
                 34 
                 RSAD2 
                 91543 
                 0.0081 
               
               
                   
                 35 
                 ESR1 
                 2099 
                 0.0079 
               
               
                   
                 36 
                 IKZF3 
                 22806 
                 0.0073 
               
               
                   
                 37 
                 OR2I1P 
                 442197 
                 0.007 
               
               
                   
                 38 
                 EGFR 
                 1956 
                 −0.0066 
               
               
                   
                 39 
                 NAT1 
                 9 
                 0.0065 
               
               
                   
                 40 
                 LATS2 
                 26524 
                 −0.0063 
               
               
                   
                 41 
                 CYP2B6 
                 1555 
                 0.0061 
               
               
                   
                 42 
                 PTPRC 
                 5788 
                 0.0051 
               
               
                   
                 43 
                 PPP1R1A 
                 5502 
                 −0.0041 
               
               
                   
                 44 
                 AL137218.1 
                 N/A 
                 −0.0017 
               
               
                   
                   
               
            
           
         
       
     
     Table 3 presents the probe sets from the Xcel Array (Almac) that represent the genes in Table 2A and 2B with reference to their sequence ID numbers. 
     
       
         
           
               
             
               
                 TABLE 3 
               
             
            
               
                   
               
               
                 Probe set IDs and SEQ Numbers for target sequences of genes  
               
               
                 contained in 44-gene signature as mapped to Xcel platform 
               
            
           
           
               
               
               
               
            
               
                   
                   
                   
                 SEQ ID NO: 
               
               
                   
                 Gene 
                 Probeset ID 
                 of Target sequence 
               
               
                   
                   
               
               
                   
                 AC138128.1 
                 NONMATCH 
                 #N/A 
               
               
                   
                 ADAMTS4 
                 ADXEC.29185.C1_at 
                 51 
               
               
                   
                 ADAMTS4 
                 ADXECAD.1557_at 
                 52 
               
               
                   
                 ADAMTS4 
                 ADXECAD.1557_x_at 
                 53 
               
               
                   
                 ADAMTS4 
                 ADXECNTDJ.9649_at 
                 54 
               
               
                   
                 AL137218.1 
                 ADXECADA.15298_x_at 
                 55 
               
               
                   
                 ANXA1 
                 ADXEC.961.C1_at 
                 56 
               
               
                   
                 ANXA1 
                 ADXEC.961.C2_s_at 
                 57 
               
               
                   
                 ANXA1 
                 ADXEC.961.C3_at 
                 58 
               
               
                   
                 ANXA1 
                 ADXECAD.8396_at 
                 59 
               
               
                   
                 APOL3 
                 ADXEC.11171.C1_s_at 
                 60 
               
               
                   
                 CD109 
                 ADXEC.11145.C1_s_at 
                 61 
               
               
                   
                 CD109 
                 ADXEC.11777.C1_at 
                 62 
               
               
                   
                 CD109 
                 ADXEC.12292.C1_at 
                 63 
               
               
                   
                 CD2 
                 ADXEC.7301.C1-a_s_at 
                 64 
               
               
                   
                 CD2 
                 ADXEC.7301.C1_at 
                 65 
               
               
                   
                 CD2 
                 ADXECEMUTR.6872_at 
                 66 
               
               
                   
                 CD2 
                 ADXECRS.12205_s_at 
                 67 
               
               
                   
                 CD274 
                 ADXEC.11136.C1_at 
                 68 
               
               
                   
                 CD274 
                 ADXEC.23232.C1_at 
                 69 
               
               
                   
                 CD274 
                 ADXECNTDJ.4196_s_at 
                 70 
               
               
                   
                 CD274 
                 ADXECNTDJ.4198_s_at 
                 71 
               
               
                   
                 CDR1 
                 ADXECRS.7695_s_at 
                 72 
               
               
                   
                 CLDN10 
                 ADXEC.19503.C1_s_at 
                 73 
               
               
                   
                 CLDN10 
                 ADXECEMUTR.6957_at 
                 74 
               
               
                   
                 CLDN10 
                 ADXECRS.17517_s_at 
                 75 
               
               
                   
                 CXCL10 
                 ADXEC.11676.C1_at 
                 76 
               
               
                   
                 CYP2B6 
                 ADXEC.20112.C1_s_at 
                 77 
               
               
                   
                 CYP2B6 
                 ADXECAD.18663_x_at 
                 78 
               
               
                   
                 CYP2B6 
                 ADXLCEC.9263.C1_at 
                 79 
               
               
                   
                 EGFR 
                 ADXEC.14093.C1_at 
                 80 
               
               
                   
                 EGFR 
                 ADXEC.1866.C1_at 
                 81 
               
               
                   
                 EGFR 
                 ADXEC.1866.C1_x_at 
                 82 
               
               
                   
                 EGFR 
                 ADXEC.21483.C1_at 
                 83 
               
               
                   
                 EGFR 
                 ADXEC.23775.C1_at 
                 84 
               
               
                   
                 EGFR 
                 ADXEC.31869.C1_at 
                 85 
               
               
                   
                 EGFR 
                 ADXEC.4451.C1_at 
                 86 
               
               
                   
                 EGFR 
                 ADXECAD.18126_at 
                 87 
               
               
                   
                 EGFR 
                 ADXECAD.19259_at 
                 88 
               
               
                   
                 EGFR 
                 ADXECADA.15206_at 
                 89 
               
               
                   
                 EGFR 
                 ADXECADA.21225_s_at 
                 90 
               
               
                   
                 EGFR 
                 ADXECADA.8307_at 
                 91 
               
               
                   
                 EGFR 
                 ADXECEMUTR.2965_at 
                 92 
               
               
                   
                 EGFR 
                 ADXECEMUTR.3575_at 
                 93 
               
               
                   
                 EGFR 
                 ADXECNTDJ.6255_at 
                 94 
               
               
                   
                 EGFR 
                 ADXECNTDJ.6256_at 
                 95 
               
               
                   
                 EGFR 
                 ADXECNTDJ.6256_x_at 
                 96 
               
               
                   
                 EGFR 
                 ADXECRS.19907_at 
                 97 
               
               
                   
                 EGFR 
                 ADXECRS.19907_s_at 
                 98 
               
               
                   
                 EGFR 
                 ADXECRS.24032_at 
                 99 
               
               
                   
                 EGFR 
                 ADXLCEC.7900.C1_at 
                 100 
               
               
                   
                 EGFR 
                 ADXPCEC.14538.C1_at 
                 101 
               
               
                   
                 EGR1 
                 ADXEC.2432.C2_s_at 
                 102 
               
               
                   
                 EGR1 
                 ADXEC.2432.C4_at 
                 103 
               
               
                   
                 EGR1 
                 ADXEC.2432.C6-a_s_at 
                 104 
               
               
                   
                 ESR1 
                 ADXEC.27541.C1_at 
                 105 
               
               
                   
                 ESR1 
                 ADXEC.29140.C1_s_at 
                 106 
               
               
                   
                 ESR1 
                 ADXEC.33997.C1_at 
                 107 
               
               
                   
                 ESR1 
                 ADXECAD.12370_s_at 
                 108 
               
               
                   
                 ESR1 
                 ADXECAD.18631_at 
                 109 
               
               
                   
                 ESR1 
                 ADXECAD.24092_s_at 
                 110 
               
               
                   
                 ESR1 
                 ADXECADA.11317_s_at 
                 111 
               
               
                   
                 ESR1 
                 ADXECADA.9299_at 
                 112 
               
               
                   
                 ESR1 
                 ADXECNTDJ.3778_at 
                 113 
               
               
                   
                 ESR1 
                 ADXECNTDJ.3779_at 
                 114 
               
               
                   
                 ESR1 
                 ADXOCEC.10271.C1_at 
                 115 
               
               
                   
                 ESR1 
                 ADXOCEC.10271.C1_x_at 
                 116 
               
               
                   
                 ESR1 
                 ADXOCEC.9813.C1_at 
                 117 
               
               
                   
                 ETV7 
                 ADXEC.745.C1_s_at 
                 118 
               
               
                   
                 ETV7 
                 ADXECEMUTR.534_s_at 
                 119 
               
               
                   
                 FAM19A5 
                 ADXEC.10689.C1_at 
                 120 
               
               
                   
                 FAM19A5 
                 ADXEC.13789.C1_at 
                 121 
               
               
                   
                 FAM19A5 
                 ADXEC.13789.C1_s_at 
                 122 
               
               
                   
                 FAM19A5 
                 ADXEC.13789.C1_x_at 
                 123 
               
               
                   
                 FAM19A5 
                 ADXECADA.11183_at 
                 124 
               
               
                   
                 FAM19A5 
                 ADXECADA.11183_s_at 
                 125 
               
               
                   
                 FAM19A5 
                 ADXECADA.11183_x_at 
                 126 
               
               
                   
                 FAM19A5 
                 ADXECNTDJ.10271_at 
                 127 
               
               
                   
                 FOSB 
                 ADXEC.34273.C1_at 
                 128 
               
               
                   
                 FOSB 
                 ADXEC.34273.C1_x_at 
                 129 
               
               
                   
                 FOSB 
                 ADXEC.9157.C1-a_s_at  
                 130 
               
               
                   
                 FOSB 
                 ADXEC.9157.C1_at 
                 131 
               
               
                   
                 FOSB 
                 ADXECNTDJ.4222_s_at 
                 132 
               
               
                   
                 FOSB 
                 ADXECNTDJ.4223_at 
                 133 
               
               
                   
                 FOSB 
                 ADXECNTDJ.4223_x_at 
                 134 
               
               
                   
                 FOSB 
                 ADXPCEC.11652.C1_x_at 
                 135 
               
               
                   
                 FYB 
                 ADXECAD.24300_s_at 
                 136 
               
               
                   
                 FYB 
                 ADXECADA.2898_at 
                 137 
               
               
                   
                 FYB 
                 ADXECNTDJ.82_s_at 
                 138 
               
               
                   
                 GBP5 
                 ADXEC.6891.C2_at 
                 139 
               
               
                   
                 GBP5 
                 ADXEC.6891.C2_s_at 
                 140 
               
               
                   
                 GBP5 
                 ADXEC.8878.C1_at 
                 141 
               
               
                   
                 GRB14 
                 ADXEC.13641.C1_s_at 
                 142 
               
               
                   
                 IDO1 
                 ADXEC.20415.C1-a_s_at 
                 143 
               
               
                   
                 IFI44L 
                 ADXEC.30980.C1_at 
                 144 
               
               
                   
                 IFI44L 
                 ADXEC.30980.C1_x_at 
                 145 
               
               
                   
                 IFI44L 
                 ADXEC.6079.C1_at 
                 146 
               
               
                   
                 IFI44L 
                 ADXEC.6079.Cl_x_at 
                 147 
               
               
                   
                 IFI44L 
                 ADXOCEC.12110.C2_s_at 
                 148 
               
               
                   
                 IFI44L 
                 ADXOCEC.9547.C1_at 
                 149 
               
               
                   
                 IFI44L 
                 ADXOCEC.9547.C1_x_at  
                 150 
               
               
                   
                 IKZF3 
                 ADXEC.22688.C1_at 
                 151 
               
               
                   
                 IKZF3 
                 ADXEC.32096.C1_at 
                 152 
               
               
                   
                 IKZF3 
                 ADXEC.32096.C1_x_at 
                 153 
               
               
                   
                 IKZF3 
                 ADXECAD.25262_s_at 
                 154 
               
               
                   
                 IKZF3 
                 ADXECADA.10727_at 
                 155 
               
               
                   
                 IKZF3 
                 ADXECRS.658_s_at 
                 156 
               
               
                   
                 ITGAL 
                 ADXEC.7237.C1_s_at 
                 157 
               
               
                   
                 ITGAL 
                 ADXECADA.387_x_at 
                 158 
               
               
                   
                 KIF26A 
                 ADXEC.10112.C1_at 
                 159 
               
               
                   
                 KIF26A 
                 ADXEC.19112.C1_s_at 
                 160 
               
               
                   
                 KLHDC7B 
                 ADXEC.11833.C1_at 
                 161 
               
               
                   
                 KLHDC7B 
                 ADXECADA.94_at 
                 162 
               
               
                   
                 LATS2 
                 ADXEC.11588.C1_s_at 
                 163 
               
               
                   
                 LATS2 
                 ADXEC.8316.C2_s_at 
                 164 
               
               
                   
                 LATS2 
                 ADXECAD.19393_at 
                 165 
               
               
                   
                 LRP4 
                 ADXEC.13953.C1_at 
                 166 
               
               
                   
                 LRP4 
                 ADXEC.15783.C1_at 
                 167 
               
               
                   
                 LRP4 
                 ADXECADA.18233_at 
                 168 
               
               
                   
                 MFAP5 
                 ADXEC.18200.C1_at 
                 169 
               
               
                   
                 MFAP5 
                 ADXEC.8579.C1-a_s_at 
                 170 
               
               
                   
                 MFAP5 
                 ADXEC.8579.C1_at 
                 171 
               
               
                   
                 MFAP5 
                 ADXEC.8579.C2_s_at 
                 172 
               
               
                   
                 MX1 
                 ADXEC.6683.C1_at 
                 173 
               
               
                   
                 MX1 
                 ADXEC.6683.C1_s_at 
                 174 
               
               
                   
                 MX1 
                 ADXEC.6842.C2_at 
                 175 
               
               
                   
                 MX1 
                 ADXEC.6842.C2_x_at 
                 176 
               
               
                   
                 NAT1 
                 ADXEC.20034.C1-a_s_at 
                 177 
               
               
                   
                 NAT1 
                 ADXEC.20034.C1_at 
                 178 
               
               
                   
                 NAT1 
                 ADXEC.20034.C2_s_at 
                 179 
               
               
                   
                 NAT1 
                 ADXECEMUTR.4521_s_at 
                 180 
               
               
                   
                 NAT1 
                 ADXECNTDJ.5862_s_at 
                 181 
               
               
                   
                 NAT1 
                 ADXECNTDJ.5864_s_at 
                 182 
               
               
                   
                 NAT1 
                 ADXECNTDJ.5866_s_at 
                 183 
               
               
                   
                 NAT1 
                 ADXECNTDJ.5867_at 
                 184 
               
               
                   
                 NAT1 
                 ADXECNTDJ.5868_at 
                 185 
               
               
                   
                 NLRC5 
                 ADXEC.23051.C1_s_at 
                 186 
               
               
                   
                 NLRC5 
                 ADXEC.5068.C1_at 
                 187 
               
               
                   
                 NLRC5 
                 ADXECEMUTR.5074_at 
                 188 
               
               
                   
                 NLRC5 
                 ADXECEMUTR.5074_s_at 
                 189 
               
               
                   
                 NLRC5 
                 ADXECNTDJ.5048_s_at  
                 190 
               
               
                   
                 OLFM4 
                 ADXEC.8457.C1-a_s_at 
                 191 
               
               
                   
                 OLFM4 
                 ADXEC.8457.C1_s_at 
                 192 
               
               
                   
                 OR2I1P 
                 ADXECAD.16836_at 
                 193 
               
               
                   
                 OR2I1P 
                 ADXECAD.16836_s_at 
                 194 
               
               
                   
                 PI15 
                 ADXEC.29833.C1-a_s_at 
                 195 
               
               
                   
                 PI15 
                 ADXEC.29833.C1_at 
                 196 
               
               
                   
                 PI15 
                 ADXEC.29833.C1_s_at 
                 197 
               
               
                   
                 PI15 
                 ADXEC.7703.C1_at 
                 198 
               
               
                   
                 PI15 
                 ADXEC.7703.C1_x_at 
                 199 
               
               
                   
                 PI15 
                 ADXECAD.23062_at 
                 200 
               
               
                   
                 PPP1R1A 
                 ADXEC.14340.C1_at 
                 201 
               
               
                   
                 PPP1R1A 
                 ADXEC.15744.C1_at 
                 202 
               
               
                   
                 PRAME 
                 ADXEC.11333.C1_at 
                 203 
               
               
                   
                 PRAME 
                 ADXEC.11333.C1_x_at 
                 204 
               
               
                   
                 PRICKLE1 
                 ADXEC.9436.C1_at 
                 205 
               
               
                   
                 PRICKLE1 
                 ADXEC.9436.C1_x_at 
                 206 
               
               
                   
                 PRICKLE1 
                 ADXECAD.6243_s_at 
                 207 
               
               
                   
                 PRICKLE1 
                 ADXECAD.8320_at 
                 208 
               
               
                   
                 PRICKLE1 
                 ADXECRS.11172_s_at 
                 209 
               
               
                   
                 PRICKLE1 
                 ADXECRS.18104_s_at 
                 210 
               
               
                   
                 PTPRC 
                 ADXEC.8915.C1-a_s_at 
                 211 
               
               
                   
                 PTPRC 
                 ADXEC.8915.C1_at 
                 212 
               
               
                   
                 PTPRC 
                 ADXECAD.17697_at 
                 213 
               
               
                   
                 PTPRC 
                 ADXECADA.4026_at 
                 214 
               
               
                   
                 PTPRC 
                 ADXECADA.52_at 
                 215 
               
               
                   
                 PTPRC 
                 ADXECNTDJ.2722_s_at 
                 216 
               
               
                   
                 PTPRC 
                 ADXECNTDJ.2723_s_at 
                 217 
               
               
                   
                 RAC2 
                 ADXEC.15369.C1_s_at 
                 218 
               
               
                   
                 RSAD2 
                 ADXEC.8308.C1-a_s_at 
                 219 
               
               
                   
                 RSAD2 
                 ADXEC.8308.C1_at 
                 220 
               
               
                   
                 RSAD2 
                 ADXECAD.11200_at 
                 221 
               
               
                   
                 RSAD2 
                 ADXECADA.13258_s_at 
                 222 
               
               
                   
                 RSAD2 
                 ADXECNTDJ.5191_at 
                 223 
               
               
                   
                 RSAD2 
                 ADXECRS.4576_s_at 
                 224 
               
               
                   
                 SP140L 
                 ADXEC.31390.C1_at 
                 225 
               
               
                   
                 SP140L 
                 ADXECADA.3222_at 
                 226 
               
               
                   
                 TSPAN7 
                 ADXEC.12786.C1_at 
                 227 
               
               
                   
                 TSPAN7 
                 ADXECADA.9258_at 
                 228 
               
               
                   
                 TSPAN7 
                 ADXECADA.9258_x_at 
                 229 
               
               
                   
                 TSPAN7 
                 ADXECNTDJ.7964_at 
                 230 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 4 
               
             
            
               
                   
               
               
                 Gene Symbols for 44-gene DDRD classifier model  
               
               
                 with associated weightings and bias 
               
            
           
           
               
               
               
               
            
               
                   
                 Gene symbol 
                 Weight 
                 Bias 
               
               
                   
                   
               
            
           
           
               
               
               
               
            
               
                   
                 AC138128.1 
                 −0.0136 
                 1.4071 
               
               
                   
                 ADAMTS4 
                 −0.0085 
                 1.9569 
               
               
                   
                 AL137218.1 
                 −0.0017 
                 −1.1744 
               
               
                   
                 ANXA1 
                 −0.0082 
                 2.0015 
               
               
                   
                 APOL3 
                 0.0151 
                 2.2036 
               
               
                   
                 CD109 
                 −0.0129 
                 0.9477 
               
               
                   
                 CD2 
                 0.0190 
                 4.0904 
               
               
                   
                 CD274 
                 0.0133 
                 1.3730 
               
               
                   
                 CDR1 
                 −0.0149 
                 4.7979 
               
               
                   
                 CLDN10 
                 −0.0086 
                 −0.3446 
               
               
                   
                 CXCL10 
                 0.0230 
                 2.0393 
               
               
                   
                 CYP2B6 
                 0.0061 
                 0.9218 
               
               
                   
                 EGFR 
                 −0.0066 
                 −0.1767 
               
               
                   
                 EGR1 
                 −0.0086 
                 2.1865 
               
               
                   
                 ESR1 
                 0.0079 
                 0.8512 
               
               
                   
                 ETV7 
                 0.0124 
                 1.4678 
               
               
                   
                 FAM19A5 
                 −0.0110 
                 0.4137 
               
               
                   
                 FOSB 
                 −0.0111 
                 1.8589 
               
               
                   
                 FYB 
                 0.0149 
                 1.5618 
               
               
                   
                 GBP5 
                 0.0181 
                 1.3977 
               
               
                   
                 GRB14 
                 0.0137 
                 0.2696 
               
               
                   
                 IDO1 
                 0.0221 
                 0.7257 
               
               
                   
                 IFI44L 
                 0.0191 
                 1.1758 
               
               
                   
                 IKZF3 
                 0.0073 
                 −0.5899 
               
               
                   
                 ITGAL 
                 0.0176 
                 3.2161 
               
               
                   
                 KIF26A 
                 −0.0136 
                 2.0504 
               
               
                   
                 KLHDC7B 
                 0.0140 
                 1.4395 
               
               
                   
                 LATS2 
                 −0.0063 
                 0.4863 
               
               
                   
                 LRP4 
                 −0.0159 
                 0.3065 
               
               
                   
                 MFAP5 
                 −0.0121 
                 2.6992 
               
               
                   
                 MX1 
                 0.0226 
                 3.4355 
               
               
                   
                 NAT1 
                 0.0065 
                 −0.7973 
               
               
                   
                 NLRC5 
                 0.0101 
                 2.2686 
               
               
                   
                 OLFM4 
                 −0.0117 
                 0.6367 
               
               
                   
                 OR2I1P 
                 0.0070 
                 −1.3024 
               
               
                   
                 PI15 
                 −0.0115 
                 0.3355 
               
               
                   
                 PPP1R1A 
                 −0.0041 
                 1.7637 
               
               
                   
                 PRAME 
                 0.0177 
                 2.2499 
               
               
                   
                 PRICKLE1 
                 −0.0089 
                 1.7702 
               
               
                   
                 PTPRC 
                 0.0051 
                 −1.1182 
               
               
                   
                 RAC2 
                 0.0148 
                 3.0364 
               
               
                   
                 RSAD2 
                 0.0081 
                 1.4489 
               
               
                   
                 SP140L 
                 0.0084 
                 0.5505 
               
               
                   
                 TSPAN7 
                 −0.0148 
                 1.6584 
               
               
                   
                   
               
            
           
         
       
     
     In different embodiments, subsets of the biomarkers listed in Tables 1A and/or 1B, Table 2A and/or Table 2B and/or Tables 3 and/or 4 may be used in the methods described herein. These subsets include but are not limited to biomarkers ranked 1-2, 1-3, 1-4, 1-5, 1-10, 1-20, 1-30, 1-40, 1-44, 6-10, 11-15, 16-20, 21-25, 26-30, 31-35, 36-40, 36-44, 11-20, 21-30, 31-40, and 31-44 in Table 2A or Table 2B. 
     In one aspect, therapeutic responsiveness is predicted in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to at least one of the biomarkers GBP5, CXCL10, IDO1 and MX1 and at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, or 36. As used herein, the term “biomarker” can refer to a gene, an mRNA, cDNA, an antisense transcript, a miRNA, a polypeptide, a protein, a protein fragment, or any other nucleic acid sequence or polypeptide sequence that indicates either gene expression levels or protein production levels. In some embodiments, when referring to a biomarker of CXCL10, IDO1, CD2, GBP5, PRAME, ITGAL, LRP4, APOL3, CDR1, FYB, TSPAN7, RAC2, KLHDC7B, GRB14, AC138128.1, KIF26A, CD274, ETV7, MFAP5, OLFM4, PI15, FOSB, FAM19A5, NLRC5, PRICKLE1, EGR1, CLDN10, ADAMTS4, SP140L, ANXA1, RSAD2, ESR1, IKZF3, OR2I1P, EGFR, NAT1, LATS2, CYP2B6, PTPRC, PPP1R1A, or AL137218.1, the biomarker comprises an mRNA of CXCL10, IDO1, CD2, GBP5, PRAME, ITGAL, LRP4, APOL3, CDR1, FYB, TSPAN7, RAC2, KLHDC7B, GRB14, AC138128.1, KIF26A, CD274, ETV7, MFAP5, OLFM4, PI15, FOSB, FAM19A5, NLRC5, PRICKLE1, EGR1, CLDN10, ADAMTS4, SP140L, ANXA1, RSAD2, ESR1, IKZF3, OR2I1P, EGFR, NAT1, LATS2, CYP2B6, PTPRC, PPP1R1A, or AL137218.1, respectively. In further or other embodiments, when referring to a biomarker of MX1, GBP5, IFI44L, BIRC3, IGJ, IQGAP3, LOC100294459, SIX1, SLC9A3R1, STAT1, TOB1, UBD, C1QC, C2orf14, EPSTI, GALNT6, HIST1H4H, HIST2H4B, KIAA1244, LOC100287927, LOC100291682, or LOC100293679, the biomarker comprises an antisense transcript of MX1, IFI44L, GBP5, BIRC3, IGJ, IQGAP3, LOC100294459, SIX1, SLC9A3R1, STAT1, TOB1, UBD, C1QC, C2orf14, EPSTI, GALNT6, HIST1H4H, HIST2H4B, KIAA1244, LOC100287927, LOC100291682, or LOC100293679, respectively. 
     In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarkers GBP5, CXCL10, IDO1 and MX1 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, or 36. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker GBP5 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker CXCL10 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker IDO1 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker MX-1 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 or 39. 
     In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to at least two of the biomarkers CXCL10, MX1, IDO1 and IFI44L and at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarkers CXCL10, MX1, IDO1 and IFI44L and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker CXCL10 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42 or 43. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker MX1 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42 or 43. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker IDO1 and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42 or 43. In a further aspect, therapeutic responsiveness is predicted, or a cancer diagnosis is indicated, in an individual by conducting an assay on a test (biological) sample from the individual and detecting biomarker values that each correspond to the biomarker IFI44L and one of at least N additional biomarkers selected from the list of biomarkers in Table 2B, wherein N equals 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 29, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42 or 43. 
     In other embodiments, the target sequences listed in Tables 1A, 1B and/or 3, or subsets thereof, may be used in the methods described herein. The target sequences may be utilised for the purposes of designing primers and/or probes which hybridize to the target sequences. Design of suitable primers and/or probes is within the capability of one skilled in the art once the target sequence is identified. Various primer design tools are freely available to assist in this process, such as the NCBI Primer-BLAST tool; see Ye et al, BMC Bioinformatics. 13:134 (2012). The primers and/or probes may be designed such that they hybridize to the target sequence under stringent conditions (as defined herein). Primers and/or probes may be at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 (or more) nucleotides in length. It should be understood that each subset can include multiple primers and/or probes directed to the same biomarker. The tables show in some cases multiple target sequences within the same overall gene. Such primers and/or probes may be included in kits useful for performing the methods of the invention. The kits may be array or PCR based kits for example and may include additional reagents, such as a polymerase and/or dNTPs for example. 
     Measuring Gene Expression Using Classifier Models 
     A variety of methods have been utilized in an attempt to identify biomarkers and diagnose disease. For protein-based markers, these include two-dimensional electrophoresis, mass spectrometry, and immunoassay methods. For nucleic acid markers, these include mRNA expression profiles, microRNA profiles, sequencing, FISH, serial analysis of gene expression (SAGE), methylation profiles, and large-scale gene expression arrays. 
     When a biomarker indicates or is a sign of an abnormal process, disease or other condition in an individual, that biomarker is generally described as being either over-expressed or under-expressed as compared to an expression level or value of the biomarker that indicates or is a sign of a normal process, an absence of a disease or other condition in an individual. “Up-regulation”, “up-regulated”, “over-expression”, “over-expressed”, and any variations thereof are used interchangeably to refer to a value or level of a biomarker in a biological sample that is greater than a value or level (or range of values or levels) of the biomarker that is typically detected in similar biological samples from healthy or normal individuals. The terms may also refer to a value or level of a biomarker in a biological sample that is greater than a value or level (or range of values or levels) of the biomarker that may be detected at a different stage of a particular disease. 
     “Down-regulation”, “down-regulated”, “under-expression”, “under-expressed”, and any variations thereof are used interchangeably to refer to a value or level of a biomarker in a biological sample that is less than a value or level (or range of values or levels) of the biomarker that is typically detected in similar biological samples from healthy or normal individuals. The terms may also refer to a value or level of a biomarker in a biological sample that is less than a value or level (or range of values or levels) of the biomarker that may be detected at a different stage of a particular disease. 
     Further, a biomarker that is either over-expressed or under-expressed can also be referred to as being “differentially expressed” or as having a “differential level” or “differential value” as compared to a “normal” expression level or value of the biomarker that indicates or is a sign of a normal process or an absence of a disease or other condition in an individual. Thus, “differential expression” of a biomarker can also be referred to as a variation from a “normal” expression level of the biomarker. 
     The terms “differential biomarker expression” and “differential expression” are used interchangeably to refer to a biomarker whose expression is activated to a higher or lower level in a subject suffering from a specific disease, relative to its expression in a normal subject, or relative to its expression in a patient that responds differently to a particular therapy or has a different prognosis. The terms also include biomarkers whose expression is activated to a higher or lower level at different stages of the same disease. It is also understood that a differentially expressed biomarker may be either activated or inhibited at the nucleic acid level or protein level, or may be subject to alternative splicing to result in a different polypeptide product. Such differences may be evidenced by a variety of changes including mRNA levels, miRNA levels, antisense transcript levels, or protein surface expression, secretion or other partitioning of a polypeptide. Differential biomarker expression may include a comparison of expression between two or more genes or their gene products; or a comparison of the ratios of the expression between two or more genes or their gene products; or even a comparison of two differently processed products of the same gene, which differ between normal subjects and subjects suffering from a disease; or between various stages of the same disease. Differential expression includes both quantitative, as well as qualitative, differences in the temporal or cellular expression pattern in a biomarker among, for example, normal and diseased cells, or among cells which have undergone different disease events or disease stages. 
     In certain embodiments, the expression profile obtained is a genomic or nucleic acid expression profile, where the amount or level of one or more nucleic acids in the sample is determined. In these embodiments, the sample that is assayed to generate the expression profile (i.e. to measure the expression levels of the one or more biomarkers in the sample) employed in the diagnostic or prognostic methods comprises a nucleic acid sample. The nucleic acid sample includes a population of nucleic acids that includes the expression information of the phenotype determinative biomarkers of the cell or tissue being analyzed. In some embodiments, the nucleic acid may include RNA or DNA nucleic acids, e.g., mRNA, cRNA, cDNA etc., so long as the sample retains the expression information of the host cell or tissue from which it is obtained. The sample may be prepared in a number of different ways, as is known in the art, e.g., by mRNA isolation from a cell, where the isolated mRNA is used as isolated, amplified, or employed to prepare cDNA, cRNA, etc., as is known in the field of differential gene expression. Accordingly, determining the level of mRNA in a sample includes preparing cDNA or cRNA from the mRNA and subsequently measuring the cDNA or cRNA. The sample is typically prepared from a cell or tissue harvested from a subject in need of treatment, e.g., via biopsy of tissue, using standard protocols, where cell types or tissues from which such nucleic acids may be generated include any tissue in which the expression pattern of the to be determined phenotype exists, including, but not limited to, disease cells or tissue, body fluids, etc. 
     The expression profile, representing the measured expression levels of one or more biomarkers in the test sample may be generated from the initial nucleic acid sample using any convenient protocol. While a variety of different manners of generating expression profiles are known, such as those employed in the field of differential gene expression/biomarker analysis, one representative and convenient type of protocol for generating expression profiles is array-based gene expression profile generation protocols. Such applications are hybridization assays in which a surface such as a (glass) chip, on which several probes for each of several thousand genes are immobilised is employed. On these surfaces there are generally multiple target regions within each gene to be analysed, and multiple (usually from 11 to 100) probes per target region. In this way, expression of each gene is evaluated by hybridization to multiple (tens) of probes on the surface. In these assays, a sample of target nucleic acids is first prepared from the initial nucleic acid sample being assayed, where preparation may include labeling of the target nucleic acids with a label, e.g., a member of a signal producing system. Following target nucleic acid sample preparation, the sample is contacted with the array under hybridization conditions, whereby complexes are formed between target nucleic acids that are complementary to probe sequences attached to the array surface. The presence of hybridized complexes is then detected, either qualitatively or quantitatively. Specific hybridization technology which may be practiced to generate the expression profiles employed in the subject methods includes the technology described in U.S. Pat. Nos. 5,143,854; 5,288,644; 5,324,633; 5,432,049; 5,470,710; 5,492,806; 5,503,980; 5,510,270; 5,525,464; 5,547,839; 5,580,732; 5,661,028; 5,800,992; the disclosures of which are herein incorporated by reference; as well as WO 95/21265; WO 96/31622; WO 97/10365; WO 97/27317; EP 373 203; and EP 785 280. In these methods, an array of “probe” nucleic acids that includes one or several probes for each of the biomarkers whose expression is being assayed is contacted with target nucleic acids as described above. Contact is carried out under hybridization conditions, e.g., stringent hybridization conditions as described above, and unbound nucleic acid is then removed. The resultant pattern of hybridized nucleic acids provides information regarding expression for each of the biomarkers that have been probed, where the expression information is in terms of whether or not the gene is expressed and, typically, at what level, where the expression data, i.e., expression profile, may be both qualitative and quantitative. The methods may include normalizing the hybridization pattern against a subset of or all other probes on the array. 
     Creating a Biomarker Expression Classifier 
     In one embodiment, the relative expression levels of biomarkers in a cancer tissue are measured to form a gene expression profile. The gene expression profile of a set of biomarkers from a patient tissue sample is summarized in the form of a compound decision score (or test score) and compared to a score threshold that may be mathematically derived from a training set of patient data. The score threshold separates a patient group based on different characteristics such as, but not limited to, responsiveness/non-responsiveness to treatment. The patient training set data is preferably derived from OAC tissue samples having been characterized by prognosis, likelihood of recurrence, long term survival, clinical outcome, treatment response, diagnosis, cancer classification, or personalized genomics profile. Alternatively it may represent a data set from a cohort of patients in which the molecular subtype (DDRD) is well defined and characterised. Expression profiles, and corresponding decision scores from patient samples (test scores) may be correlated with the characteristics of patient samples in the training set that are on the same side of the mathematically derived score decision threshold. The threshold of the linear classifier scalar output may be optimized to maximize the sum of sensitivity and specificity under cross-validation as observed within the training dataset. Alternatively the sensitivity and positive predictive value of the assay may be increased at the expense of the specificity and negative predictive value or vice versa depending on the proposed clinical utility of the test in different disease indications. 
     The overall expression data for a given sample is normalized using methods known to those skilled in the art in order to correct for differing amounts of starting material, varying efficiencies of the extraction and amplification reactions, etc. Using a linear classifier on the normalized data to make a diagnostic or prognostic call (e.g. responsiveness or resistance to therapeutic agent) effectively means to split the data space, i.e. all possible combinations of expression values for all genes in the classifier, into two disjoint halves by means of a separating hyperplane. This split may be empirically derived on a large set of training examples, for example from patients showing responsiveness or resistance to a therapeutic agent. Without loss of generality, one can assume a certain fixed set of values for all but one biomarker, which would automatically define a threshold value for this remaining biomarker where the decision would change from, for example, responsiveness or resistance to a therapeutic agent. Expression values above this dynamic threshold would then either indicate resistance (for a biomarker with a negative weight) or responsiveness (for a biomarker with a positive weight) to a therapeutic agent. The precise value of this threshold depends on the actual measured expression profile of all other biomarkers within the classifier, but the general indication of certain biomarkers remains fixed, i.e. high values or “relative over-expression” always contributes to either a responsiveness (genes with a positive weight) or resistance (genes with a negative weight). Therefore, in the context of the overall gene expression classifier, relative expression can indicate if either up- or down-regulation of a certain biomarker is indicative of responsiveness or resistance to a therapeutic agent. 
     In one embodiment, the biomarker expression profile of a test sample, for example a patient tissue sample, is evaluated by a linear classifier. As used herein, a linear classifier refers to a weighted sum of the individual biomarker intensities into a compound decision score (“decision function”). The decision score is then compared to a pre-defined cut-off score threshold, corresponding to a certain set-point in terms of sensitivity and specificity which indicates if a sample is above the score threshold (decision function positive) or below (decision function negative). 
     Effectively, this means that the data space, i.e. the set of all possible combinations of biomarker expression values, is split into two mutually exclusive halves corresponding to different clinical classifications or predictions, e.g. one corresponding to responsiveness to a therapeutic agent and the other to resistance. In the context of the overall classifier, relative over-expression of a certain biomarker can either increase the decision score (positive weight) or reduce it (negative weight) and thus contribute to an overall decision of, for example, responsiveness or resistance to a therapeutic agent. 
     The term “area under the curve” or “AUC” refers to the area under the curve of a receiver operating characteristic (ROC) curve, both of which are well known in the art. AUC measures are useful for comparing the accuracy of a classifier across the complete data range. Classifiers with a greater AUC have a greater capacity to classify unknowns correctly between two groups of interest (e.g., OAC cancer samples and normal or control samples). ROC curves are useful for plotting the performance of a particular feature (e.g., any of the biomarkers described herein and/or any item of additional biomedical information) in distinguishing between two populations (e.g., individuals responding and not responding to a therapeutic agent). Typically, the feature data across the entire population (e.g., the cases and controls) are sorted in ascending order based on the value of a single feature. Then, for each value for that feature, the true positive and false positive rates for the data are calculated. The true positive rate is determined by counting the number of cases above the value for that feature and then dividing by the total number of cases. The false positive rate is determined by counting the number of controls above the value for that feature and then dividing by the total number of controls. Although this definition refers to scenarios in which a feature is elevated in cases compared to controls, this definition also applies to scenarios in which a feature is lower in cases compared to the controls (in such a scenario, samples below the value for that feature would be counted). ROC curves can be generated for a single feature as well as for other single outputs, for example, a combination of two or more features can be mathematically combined (e.g., added, subtracted, multiplied, etc.) to provide a single sum value, and this single sum value can be plotted in a ROC curve. Additionally, any combination of multiple features, in which the combination derives a single output value, can be plotted in a ROC curve. These combinations of features may comprise a test. The ROC curve is the plot of the true positive rate (sensitivity) of a test against the false positive rate (1-specificity) of the test. 
     The interpretation of this quantity, i.e. the cut-off threshold responsiveness or resistance to a therapeutic agent, is derived in the development phase (“training”) from a set of patients with known outcome. The corresponding weights and the responsiveness/resistance cut-off threshold for the decision score are fixed a priori from training data by methods known to those skilled in the art. In a preferred embodiment of the present method, Partial Least Squares Discriminant Analysis (PLS-DA) is used for determining the weights. (L. Ståhle, S. Wold, J. Chemom. 1 (1987) 185-196; D. V. Nguyen, D. M. Rocke, Bioinformatics 18 (2002) 39-50). Other methods for performing the classification, known to those skilled in the art, may also be used with the methods described herein, for example when applied to the transcripts of an oesophageal cancer (OAC) classifier. 
     Different methods can be used to convert quantitative data measured on these biomarkers into a prognosis or other predictive use. These methods include, but are not limited to methods from the fields of pattern recognition (Duda et al. Pattern Classification, 2 nd  ed., John Wiley, New York 2001), machine learning (Schölkopf et al. Learning with Kernels, MIT Press, Cambridge 2002, Bishop, Neural Networks for Pattern Recognition, Clarendon Press, Oxford 1995), statistics (Hastie et al. The Elements of Statistical Learning, Springer, New York 2001), bioinformatics (Dudoit et al., 2002, J. Am. Statist. Assoc. 97:77-87, Tibshirani et al., 2002, Proc. Natl. Acad. Sci. USA 99:6567-6572) or chemometrics (Vandeginste, et al., Handbook of Chemometrics and Qualimetrics, Part B, Elsevier, Amsterdam 1998). 
     In a training step, a set of patient samples for both responsiveness/resistance cases are measured and the prediction method is optimised using the inherent information from this training data to optimally predict the training set or a future sample set. In this training step, the used method is trained or parameterised to predict from a specific intensity pattern to a specific predictive call. Suitable transformation or pre-processing steps might be performed with the measured data before it is subjected to the prognostic method or algorithm. 
     In a preferred embodiment of the invention, a weighted sum of the pre-processed intensity values for each transcript is formed and compared with a threshold value optimised on the training set (Duda et al. Pattern Classification, 2 nd  ed., John Wiley, New York 2001). The weights can be derived by a multitude of linear classification methods, including but not limited to Partial Least Squares (PLS, (Nguyen et al., 2002, Bioinformatics 18 (2002) 39-50)) or Support Vector Machines (SVM, (Schölkopf et al. Learning with Kernels, MIT Press, Cambridge 2002)). 
     In another embodiment of the invention, the data is transformed non-linearly before applying a weighted sum as described above. This non-linear transformation might include increasing the dimensionality of the data. The non-linear transformation and weighted summation might also be performed implicitly, e.g. through the use of a kernel function. (Schölkopf et al. Learning with Kernels, MIT Press, Cambridge 2002). 
     In another embodiment of the invention, a new data sample is compared with two or more class prototypes, being either real measured training samples or artificially created prototypes. This comparison is performed using suitable similarity measures, for example, but not limited to Euclidean distance (Duda et al. Pattern Classification, 2 nd  ed., John Wiley, New York 2001), correlation coefficient (Van&#39;t Veer, et al. 2002, Nature 415:530) etc. A new sample is then assigned to the prognostic group with the closest prototype or the highest number of prototypes in the vicinity. 
     In another embodiment of the invention, decision trees (Hastie et al., The Elements of Statistical Learning, Springer, New York 2001) or random forests (Breiman, Random Forests, Machine Learning 45:5 2001) are used to make a prognostic call from the measured intensity data for the transcript set or their products. 
     In another embodiment of the invention neural networks (Bishop, Neural Networks for Pattern Recognition, Clarendon Press, Oxford 1995) are used to make a prognostic call from the measured intensity data for the transcript set or their products. 
     In another embodiment of the invention, discriminant analysis (Duda et al., Pattern Classification, 2 nd  ed., John Wiley, New York 2001), comprising but not limited to linear, diagonal linear, quadratic and logistic discriminant analysis, is used to make a prognostic call from the measured intensity data for the transcript set or their products. 
     In another embodiment of the invention, Prediction Analysis for Microarrays (PAM, (Tibshirani et al., 2002, Proc. Natl. Acad. Sci. USA 99:6567-6572)) is used to make a prognostic call from the measured intensity data for the transcript set or their products. 
     In another embodiment of the invention, Soft Independent Modelling of Class Analogy (SIMCA, (Wold, 1976, Pattern Recogn. 8:127-139)) is used to make a predictive call from the measured intensity data for the transcript set or their products. 
     In another embodiment of the invention, c-index is used to quantify predictive ability. This index applies biomarkers to a continuous response variable that can be censored. The c index is the proportion of all pairs of subjects whose survival times can be ordered such that the subject with the higher predicted survival is the one who survived longer. Two subject&#39;s survival times cannot be ordered if both subjects are censored or if one has failed and the follow up time of the other is less than the failure time of the first. The c index is the probability of concordance between predicted and observed survival, with c=0.5 for random prediction and c=1 for a perfectly discriminating model. (Frank E. Harrell, Jr. Regression Modeling Strategies, 2001). 
     Therapeutic Agents 
     As described above, the methods described herein permit the classification of a patient suffering from OAC, including early stage OAC as responsive or non-responsive to a therapeutic agent that targets tumors with abnormal DNA repair (hereinafter referred to as a “DNA-damaging therapeutic agent”). As used herein “DNA-damaging therapeutic agent” includes agents known to damage DNA directly, agents that prevent DNA damage repair, agents that inhibit DNA damage signaling, agents that inhibit DNA damage induced cell cycle arrest, and agents that inhibit processes indirectly leading to DNA damage. Some current such therapeutics used to treat OAC include, but are not limited to, the following DNA-damaging therapeutic agents. 
     1) DNA Damaging Agents:
         a. Alkylating agents (platinum containing agents such as cisplatin, carboplatin, and oxaliplatin; cyclophosphamide; busulphan).   b. Topoisomerase I inhibitors (irinotecan;topotecan)   c. Topoisomerase II inhibitors (etoposide;anthracyclines such as doxorubicin and epirubicin)   d. Ionising radiation       

     2) DNA Repair Targeted Therapies
         a. Inhibitors of Non-homologous end-joining (DNA-PK inhibitors, Nu7441, NU7026)   b. Inhibitors of homologous recombination   c. Inhibitors of nucleotide excision repair   d. Inhibitors of base excision repair (PARP inhibitors, AG014699, AZD2281, ABT-888, MK4827, BSI-201, INO-1001, TRC-102, APEX 1 inhibitors, APEX 2 inhibitors, Ligase III inhibitors   e. Inhibitors of the Fanconi anemia pathway       

     3) Inhibitors of DNA Damage Signalling
         a. ATM inhibitors (CP466722)   b. CHK 1 inhibitors (XL-844,UCN-01, AZD7762, PF00477736)   c. CHK 2 inhibitors (XL-844, AZD7762, PF00477736)   d. ATR inhibitors (AZ20)       

     4) Inhibitors of DNA Damage Induced Cell Cycle Arrest
         a. Wee1 kinase inhibitors   b. CDC25a, b or c inhibitors       

     5) Inhibition of Processes Indirectly Leading to DNA Damage
         a. Histone deacetylase inhibitors   b. Heat shock protein inhibitors (geldanamycin, AUY922),       

     6) Inhibitors of DNA Synthesis:
         a. Pyrimidine analogues (5-FU, gemcitabine)   b. Prodrugs (capecitabine)       

     As discussed above the therapeutic agents for which responsiveness is predicted may be applied in a neoadjuvant setting. However, they may be utilised in an adjuvant setting additionally or alternatively. 
     The invention described herein is not limited to any one DNA-damaging therapeutic agent; it can be used to identify responders and non-responders to any of a range of DNA-damaging therapeutic agent, for example those that directly or indirectly affect DNA damage and/or DNA damage repair. In some embodiments, the DNA-damaging therapeutic agent comprises one or more substances selected from the group consisting of: a DNA damaging agent, a DNA repair targeted therapy, an inhibitor of DNA damage signalling, an inhibitor of DNA damage induced cell cycle arrest, a histone deacetylase inhibitor, a heat shock protein inhibitor and an inhibitor of DNA synthesis. More specifically, the DNA-damaging therapeutic agent may be selected from one or more of a platinum-containing agent, a nucleoside analogue such as gemcitabine or 5-fluorouracil or a prodrug thereof such as capecitabine, an anthracycline such as epirubicin or doxorubicin, an alkylating agent such as cyclophosphamide, an ionising radiation or a combination of (ionising) radiation and chemotherapy (chemoradiation). In particular embodiments, the DNA-damaging therapeutic agent comprises a platinum-containing agent, such as a platinum based agent selected from cisplatin, carboplatin and oxaliplatin. The methods and kits may predict responsiveness to treatment with the DNA-damaging therapeutic agent together with a further therapy (such as radiation) or drug. Thus, the methods and kits may predict responsiveness to a combination therapy. In some embodiments, the further drug is a mitotic inhibitor. The mitotic inhibitor may be a  vinca  alkaloid or a taxane. In specific embodiments, the  vinca  alkaloid is vinorelbine. In other embodiments, the taxane is paclitaxel or docetaxel. In certain embodiments, responders to the following treatments are identified: cisplatin/carboplatin and 5-fluorouracil (CF), cisplatin/carboplatin and capecitabine (CX), epirubicin/doxorubicin, cisplatin/carboplatin and fluorouracil (ECF), epirubicin/doxorubicin, oxaliplatin and capecitabine (EOX), and combinations with paclitaxel, irinotecan and vinorelbine, radiation or chemoradiation with or without taxanes, in treatment of OAC. 
     Diseases and Tissue Sources 
     The predictive classifiers described herein are useful for determining responsiveness or resistance to a therapeutic agent for treating oesophageal cancer, in particular OAC. The oesophageal cancer is typically oesophageal adenocarcinoma (OAC) and may be early stage. 
     In one embodiment, the methods described herein refer to OACs that are treated with chemotherapeutic agents of the classes DNA damaging agents, DNA repair target therapies, inhibitors of DNA damage signalling, inhibitors of DNA damage induced cell cycle arrest, inhibition of processes indirectly leading to DNA damage and inhibition of DNA synthesis, but not limited to these classes. Each of these chemotherapeutic agents is considered a “DNA-damaging therapeutic agent” as the term is used herein. 
     “Biological sample”, “sample”, and “test sample” are used interchangeably herein to refer to any material, biological fluid, tissue, or cell obtained or otherwise derived from an individual. This includes blood (including whole blood, leukocytes, peripheral blood mononuclear cells, buffy coat, plasma, and serum), sputum, tears, mucus, nasal washes, nasal aspirate, breath, urine, semen, saliva, meningeal fluid, amniotic fluid, glandular fluid, lymph fluid, nipple aspirate, bronchial aspirate, synovial fluid, joint aspirate, ascites, cells, a cellular extract, and cerebrospinal fluid. This also includes experimentally separated fractions of all of the preceding. For example, a blood sample can be fractionated into serum or into fractions containing particular types of blood cells, such as red blood cells or white blood cells (leukocytes). If desired, a sample can be a combination of samples from an individual, such as a combination of a tissue and fluid sample. The term “biological sample” also includes materials containing homogenized solid material, such as from a stool sample, a tissue sample, or a tissue biopsy, for example. The term “biological sample” also includes materials derived from a tissue culture or a cell culture. Any suitable methods for obtaining a biological sample can be employed; exemplary methods include, e.g., phlebotomy, swab (e.g., buccal swab), and a fine needle aspirate biopsy procedure. Samples may be obtained by endoscopy in some embodiments. A “biological sample” obtained or derived from an individual includes any such sample that has been processed in any suitable manner after being obtained from the individual. 
     In such cases, the target cells may be tumor cells, for example OAC cells. The target cells are derived from any tissue source, including human and animal tissue, such as, but not limited to, a newly obtained sample, a frozen sample, a biopsy sample, a sample of bodily fluid, a blood sample, preserved tissue such as a paraffin-embedded fixed tissue sample (i.e., a tissue block), or cell culture. 
     In some specific embodiments, the samples may or may not comprise vesicles. 
     Methods and Kits 
     Kits for Gene Expression Analysis 
     Reagents, tools, and/or instructions for performing the methods described herein can be provided in a kit. For example, the kit can contain reagents, tools, and instructions for determining an appropriate therapy for a oesophageal cancer patient. Such a kit can include reagents for collecting a tissue sample from a patient, such as by biopsy, and reagents for processing the tissue. The kit can also include one or more reagents for performing a biomarker expression analysis, such as reagents for performing nucleic acid amplification, including RT-PCR and qPCR, NGS, northern blot, proteomic analysis, or immunohistochemistry to determine expression levels of biomarkers in a sample of a patient. For example, primers for performing RT-PCR, probes for performing northern blot analyses, and/or antibodies for performing proteomic analysis such as Western blot, immunohistochemistry and ELISA analyses can be included in such kits. Appropriate buffers for the assays can also be included. Detection reagents required for any of these assays can also be included. The appropriate reagents and methods are described in further detail herein. 
     In certain embodiments, the target sequences listed in Tables 1A (SEQ ID NO: 1-24), 1B (SEQ ID NO: 25-50) and/or 3 (SEQ ID NO: 51-230), or subsets thereof, may be used in the methods and kits described herein. The target sequences may be utilised for the purposes of designing primers and/or probes which hybridize to the target sequences. Design of suitable primers and/or probes is within the capability of one skilled in the art once the target sequence is identified. Various primer design tools are freely available to assist in this process such as the NCBI Primer-BLAST tool. The primers and/or probes may be designed such that they hybridize to the target sequence under stringent conditions. Primers and/or probes may be at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 (or more) nucleotides in length. It should be understood that each subset can include multiple primers and/or probes directed to the same biomarker. The tables show in some cases multiple target sequences within the same overall gene. Such primers and/or probes may be included in kits useful for performing the methods of the invention. The kits may be array or PCR based kits for example and may include additional reagents, such as a polymerase and/or dNTPs for example. The kits featured herein can also include an instruction sheet describing how to perform the assays for measuring biomarker expression. The instruction sheet can also include instructions for how to determine a reference cohort, including how to determine expression levels of biomarkers in the reference cohort and how to assemble the expression data to establish a reference for comparison to a test patient. The instruction sheet can also include instructions for assaying biomarker expression in a test patient and for comparing the expression level with the expression in the reference cohort to subsequently determine the appropriate chemotherapy for the test patient. Methods for determining the appropriate chemotherapy are described above and can be described in detail in the instruction sheet. 
     Informational material included in the kits can be descriptive, instructional, marketing or other material that relates to the methods described herein and/or the use of the reagents for the methods described herein. For example, the informational material of the kit can contain contact information, e.g., a physical address, email address, website, or telephone number, where a user of the kit can obtain substantive information about performing a gene expression analysis and interpreting the results, particularly as they apply to a human&#39;s likelihood of having a positive response to a specific therapeutic agent. 
     The kits featured herein can also contain software necessary to infer a patient&#39;s likelihood of having a positive response to a specific therapeutic agent from the biomarker expression. 
     The kits may, in some embodiments, additionally contain the DNA-damaging therapeutic agent for administration in the event that the individual is predicted to be responsive. Any of the specific agents or combinations of agents described herein to treat OAC may be incorporated into the kits. The agent or combination of agents may be provided in a form, such as a dosage form, that is tailored to OAC treatment specifically. The kit may be provided with suitable instructions for administration according to OAC treatment regimens, for example in the context of neoadjuvant and/or adjuvant treatment. 
     a) Gene Expression Profiling Methods 
     Measuring mRNA in a biological sample may be used as a surrogate for detection of the level of the corresponding protein in the biological sample. Thus, any of the biomarkers or biomarker panels described herein can also be detected by detecting the appropriate RNA. Methods of gene expression profiling include, but are not limited to, microarray, RT-PCT, qPCR, NGS, northern blots, SAGE, mass spectrometry. 
     mRNA expression levels may be measured by reverse transcription quantitative polymerase chain reaction (RT-PCR followed with qPCR). RT-PCR is used to create a cDNA from the mRNA. The cDNA may be used in a qPCR assay to produce fluorescence as the DNA amplification process progresses. By comparison to a standard curve, qPCR can produce an absolute measurement such as number of copies of mRNA per cell. Northern blots, microarrays, Invader assays, and RT-PCR combined with capillary electrophoresis have all been used to measure expression levels of mRNA in a sample. See Gene Expression Profiling: Methods and Protocols, Richard A. Shimkets, editor, Humana Press, 2004. 
     miRNA molecules are small RNAs that are non-coding but may regulate gene expression. Any of the methods suited to the measurement of mRNA expression levels can also be used for the corresponding miRNA. Recently many laboratories have investigated the use of miRNAs as biomarkers for disease. Many diseases involve widespread transcriptional regulation, and it is not surprising that miRNAs might find a role as biomarkers. The connection between miRNA concentrations and disease is often even less clear than the connections between protein levels and disease, yet the value of miRNA biomarkers might be substantial. Of course, as with any RNA expressed differentially during disease, the problems facing the development of an in vitro diagnostic product will include the requirement that the miRNAs survive in the diseased cell and are easily extracted for analysis, or that the miRNAs are released into blood or other matrices where they must survive long enough to be measured. Protein biomarkers have similar requirements, although many potential protein biomarkers are secreted intentionally at the site of pathology and function, during disease, in a paracrine fashion. Many potential protein biomarkers are designed to function outside the cells within which those proteins are synthesized. 
     Gene expression may also be evaluated using mass spectrometry methods. A variety of configurations of mass spectrometers can be used to detect biomarker values. Several types of mass spectrometers are available or can be produced with various configurations. In general, a mass spectrometer has the following major components: a sample inlet, an ion source, a mass analyzer, a detector, a vacuum system, and instrument-control system, and a data system. Difference in the sample inlet, ion source, and mass analyzer generally define the type of instrument and its capabilities. For example, an inlet can be a capillary-column liquid chromatography source or can be a direct probe or stage such as used in matrix-assisted laser desorption. Common ion sources are, for example, electrospray, including nanospray and microspray or matrix-assisted laser desorption. Common mass analyzers include a quadrupole mass filter, ion trap mass analyzer and time-of-flight mass analyzer. Additional mass spectrometry methods are well known in the art (see Burlingame et al., Anal. Chem. 70:647 R-716R (1998); Kinter and Sherman, New York (2000)). 
     Protein biomarkers and biomarker values can be detected and measured by any of the following: electrospray ionization mass spectrometry (ESI-MS), ESI-MS/MS, ESI-MS/(MS)n, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), desorption/ionization on silicon (DIOS), secondary ion mass spectrometry (SIMS), quadrupole time-of-flight (Q-TOF), tandem time-of-flight (TOF/TOF) technology, called ultraflex III TOF/TOF, atmospheric pressure chemical ionization mass spectrometry (APCI-MS), APCI-MS/MS, APCI-(MS).sup.N, atmospheric pressure photoionization mass spectrometry (APPI-MS), APPI-MS/MS, and APPI-(MS).sup.N, quadrupole mass spectrometry, Fourier transform mass spectrometry (FTMS), quantitative mass spectrometry, and ion trap mass spectrometry. 
     Sample preparation strategies are used to label and enrich samples before mass spectroscopic characterization of protein biomarkers and determination biomarker values. Labeling methods include but are not limited to isobaric tag for relative and absolute quantitation (iTRAQ) and stable isotope labeling with amino acids in cell culture (SILAC). Capture reagents used to selectively enrich samples for candidate biomarker proteins prior to mass spectroscopic analysis include but are not limited to aptamers, antibodies, nucleic acid probes, chimeras, small molecules, an F(ab′) 2  fragment, a single chain antibody fragment, an Fv fragment, a single chain Fv fragment, a nucleic acid, a lectin, a ligand-binding receptor, affybodies, nanobodies, ankyrins, domain antibodies, alternative antibody scaffolds (e.g. diabodies etc) imprinted polymers, avimers, peptidomimetics, peptoids, peptide nucleic acids, threose nucleic acid, a hormone receptor, a cytokine receptor, and synthetic receptors, and modifications and fragments of these. 
     The foregoing assays enable the detection of biomarker values that are useful in methods for predicting responsiveness of a cancer therapeutic agent, where the methods comprise detecting, in a biological sample from an individual suffering from OAC, at least N biomarker values that each correspond to a biomarker selected from the group consisting of the biomarkers provided in Tables 1 to 4, wherein a classification, as described in detail herein, using the biomarker values indicates whether the individual will be responsive to a therapeutic agent. While certain of the described predictive biomarkers are useful alone for predicting responsiveness to a therapeutic agent, methods are also described herein for the grouping of multiple subsets of the biomarkers that are each useful as a panel of two or more biomarkers. Thus, various embodiments of the instant application provide combinations comprising N biomarkers, wherein N is at least three biomarkers. It will be appreciated that N can be selected to be any number from any of the above-described ranges, as well as similar, but higher order, ranges. In accordance with any of the methods described herein, biomarker values can be detected and classified individually or they can be detected and classified collectively, as for example in a multiplex assay format. 
     b) Microarray Methods 
     In one embodiment, the present invention makes use of “oligonucleotide arrays” (also called herein “microarrays”). Microarrays can be employed for analyzing the expression of biomarkers in a cell, and especially for measuring the expression of biomarkers of cancer tissues. 
     In one embodiment, biomarker arrays are produced by hybridizing detectably labeled polynucleotides representing the mRNA transcripts present in a cell (e.g., fluorescently-labeled cDNA synthesized from total cell mRNA or labeled cRNA) to a microarray. A microarray is a surface with an ordered array of binding (e.g., hybridization) sites for products of many of the genes in the genome of a cell or organism, preferably most or almost all of the genes. Microarrays can be made in a number of ways known in the art. However produced, microarrays share certain characteristics. The arrays are reproducible, allowing multiple copies of a given array to be produced and easily compared with each other. Preferably the microarrays are small, usually smaller than 5 cm 2 , and they are made from materials that are stable under binding (e.g., nucleic acid hybridization) conditions. A given binding site or unique set of binding sites in the microarray will specifically bind the product of a single gene in the cell. In a specific embodiment, positionally addressable arrays containing affixed nucleic acids of known sequence at each location are used. 
     It will be appreciated that when cDNA complementary to the RNA of a cell is made and hybridized to a microarray under suitable hybridization conditions, the level of hybridization to the site in the array corresponding to any particular gene will reflect the prevalence in the cell of mRNA transcribed from that gene/biomarker. For example, when detectably labeled (e.g., with a fluorophore) cDNA or cRNA complementary to the total cellular mRNA is hybridized to a microarray, the site on the array corresponding to a gene (i.e., capable of specifically binding the product of the gene) that is not transcribed in the cell will have little or no signal (e.g., fluorescent signal), and a gene for which the encoded mRNA is prevalent will have a relatively strong signal. Nucleic acid hybridization and wash conditions are chosen so that the probe “specifically binds” or “specifically hybridizes’ to a specific array site, i.e., the probe hybridizes, duplexes or binds to a sequence array site with a complementary nucleic acid sequence but does not hybridize to a site with a non-complementary nucleic acid sequence. As used herein, one polynucleotide sequence is considered complementary to another when, if the shorter of the polynucleotides is less than or equal to 25 bases, there are no mismatches using standard base-pairing rules or, if the shorter of the polynucleotides is longer than 25 bases, there is no more than a 5% mismatch. Preferably, the polynucleotides are perfectly complementary (no mismatches). It can be demonstrated that specific hybridization conditions result in specific hybridization by carrying out a hybridization assay including negative controls using routine experimentation. 
     Optimal hybridization conditions will depend on the length (e.g., oligomer vs. polynucleotide greater than 200 bases) and type (e.g., RNA, DNA, PNA) of labeled probe and immobilized polynucleotide or oligonucleotide. General parameters for specific (i.e., stringent) hybridization conditions for nucleic acids are described in Sambrook et al., supra, and in Ausubel et al., “Current Protocols in Molecular Biology”, Greene Publishing and Wiley-interscience, NY (1987), which is incorporated in its entirety for all purposes. When the cDNA microarrays are used, typical hybridization conditions are hybridization in 5×SSC plus 0.2% SDS at 65° C. for 4 hours followed by washes at 25° C. in low stringency wash buffer (1×SSC plus 0.2% SDS) followed by 10 minutes at 25° C. in high stringency wash buffer (0.1SSC plus 0.2% SDS) (see Shena et al., Proc. Natl. Acad. Sci. USA, Vol. 93, p. 10614 (1996)). Useful hybridization conditions are also provided in, e.g., Tijessen, Hybridization With Nucleic Acid Probes”, Elsevier Science Publishers B.V. (1993) and Kricka, “Nonisotopic DNA Probe Techniques”, Academic Press, San Diego, Calif. (1992). 
     Microarray platforms include those manufactured by companies such as Affymetrix, Illumina and Agilent. Examples of microarray platforms manufactured by Affymetrix include the U133 Plus2 array, the Almac proprietary Xcel™ array and the Almac proprietary Cancer DSAs®, including the Breast Cancer DSA® and Lung Cancer DSA® 
     c) Immunoassay Methods 
     Immunoassay methods are based on the reaction of an antibody to its corresponding target or analyte and can detect the analyte in a sample depending on the specific assay format. To improve specificity and sensitivity of an assay method based on immunoreactivity, monoclonal antibodies are often used because of their specific epitope recognition. Polyclonal antibodies have also been successfully used in various immunoassays because of their increased affinity for the target as compared to monoclonal antibodies Immunoassays have been designed for use with a wide range of biological sample matrices Immunoassay formats have been designed to provide qualitative, semi-quantitative, and quantitative results. 
     Quantitative results may be generated through the use of a standard curve created with known concentrations of the specific analyte to be detected. The response or signal from an unknown sample is plotted onto the standard curve, and a quantity or value corresponding to the target in the unknown sample is established. 
     Numerous immunoassay formats have been designed. ELISA or EIA can be quantitative for the detection of an analyte/biomarker. This method relies on attachment of a label to either the analyte or the antibody and the label component includes, either directly or indirectly, an enzyme. ELISA tests may be formatted for direct, indirect, competitive, or sandwich detection of the analyte. Other methods rely on labels such as, for example, radioisotopes (I 125 ) or fluorescence. Additional techniques include, for example, agglutination, nephelometry, turbidimetry, Western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, Luminex assay, and others (see ImmunoAssay: A Practical Guide, edited by Brian Law, published by Taylor &amp; Francis, Ltd., 2005 edition). 
     Exemplary assay formats include enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, fluorescent, chemiluminescence, and fluorescence resonance energy transfer (FRET) or time resolved-FRET (TR-FRET) immunoassays. Examples of procedures for detecting biomarkers include biomarker immunoprecipitation followed by quantitative methods that allow size and peptide level discrimination, such as gel electrophoresis, capillary electrophoresis, planar electrochromatography, and the like. 
     Methods of detecting and/or quantifying a detectable label or signal generating material depend on the nature of the label. The products of reactions catalyzed by appropriate enzymes (where the detectable label is an enzyme; see above) can be, without limitation, fluorescent, luminescent, or radioactive or they may absorb visible or ultraviolet light. Examples of detectors suitable for detecting such detectable labels include, without limitation, x-ray film, radioactivity counters, scintillation counters, spectrophotometers, colorimeters, fluorometers, luminometers, and densitometers. 
     Any of the methods for detection can be performed in any format that allows for any suitable preparation, processing, and analysis of the reactions. This can be, for example, in multi-well assay plates (e.g., 96 wells or 384 wells) or using any suitable array or microarray. Stock solutions for various agents can be made manually or robotically, and all subsequent pipetting, diluting, mixing, distribution, washing, incubating, sample readout, data collection and analysis can be done robotically using commercially available analysis software, robotics, and detection instrumentation capable of detecting a detectable label. 
     Clinical Uses 
     In some embodiments, methods are provided for identifying and/or selecting an OAC patient who is responsive to a therapeutic regimen. In particular, the methods are directed to identifying or selecting a cancer patient who is responsive to a therapeutic regimen that includes administering an agent that directly or indirectly damages DNA. Methods are also provided for identifying a patient who is non-responsive to a therapeutic regimen. These methods typically include determining the level of expression of a collection of predictive markers in a patient&#39;s tumor (primary, metastatic or other derivatives from the tumor such as, but not limited to, blood, or components in blood, urine, saliva and other bodily fluids)(e.g., a patient&#39;s cancer cells), comparing the level of expression to a reference expression level, and identifying whether expression in the sample includes a pattern or profile of expression of a selected predictive biomarker or biomarker set which corresponds to response or non-response to therapeutic agent. 
     In some embodiments a method of predicting responsiveness of an individual having oesophageal adenocarcinoma (OAC) to treatment with a DNA-damaging therapeutic agent comprises:
         a. measuring expression levels of one or more biomarkers in a test sample obtained from the individual, wherein the one or more biomarkers are selected from Table 1A and/or 1B and/or 2A and/or 2B and/or 3 and/or 4;   b. deriving a test score that captures the expression levels;   c. providing a threshold score comprising information correlating the test score and responsiveness;   d. and comparing the test score to the threshold score; wherein responsiveness is predicted when the test score exceeds the threshold score.       

     In specific embodiments, a method of predicting responsiveness of an individual having oesophageal adenocarcinoma (OAC) to treatment with a DNA-damaging therapeutic agent comprises the following steps: obtaining a test sample from the individual; measuring expression levels of one or more biomarkers in the test sample, wherein the one or more biomarkers are selected from the group consisting of CXCL10, MX1, IDO1, IF144L, CD2, GBP5, PRAME, ITGAL, LRP4, and APOL3; deriving a test score that captures the expression levels; providing a threshold score comprising information correlating the test score and responsiveness; and comparing the test score to the threshold score; wherein responsiveness is predicted when the test score exceeds the threshold score. One of ordinary skill in the art can determine an appropriate threshold score, and appropriate biomarker weightings and bias, using the teachings provided herein including the teachings of Example 1. 
     In other embodiments, the method of predicting responsiveness of an individual having oesophageal adenocarcinoma (OAC) to treatment with to a DNA-damaging therapeutic agent comprises measuring the expression levels of one or more biomarkers in the test sample, wherein the one or more biomarkers are selected from the group consisting of CXCL10, MX1, IDO1, IF144L, CD2, GBP5, PRAME, ITGAL, LRP4, APOL3, CDR1, FYB, TSPAN7, RAC2, KLHDC7B, GRB14, AC138128.1, KIF26A, CD274, CD109, ETV7, MFAP5, OLFM4, PI15, FOSB, FAM19A5, NLRC5, PRICKLE1, EGR1, CLDN10, ADAMTS4, SP140L, ANXA1, RSAD2, ESR1, IKZF3, OR2I1P, EGFR, NAT1, LATS2, CYP2B6, PTPRC, PPP1R1A, and AL137218.1. Tables 2A and 2B provide exemplary gene signatures (or gene classifiers) wherein the biomarkers consist of 40 or 44 of the gene products listed therein, respectively, and wherein a threshold score is derived from the individual gene product weightings listed therein. In one of these embodiments wherein the biomarkers consist of the 44 gene products listed in Table 2B, and the biomarkers are associated with the weightings provided in Table 2B, a test score that exceeds a threshold score, such as a threshold score of 0.3681 indicates a likelihood that the individual will be responsive to a DNA-damaging therapeutic agent. 
     A cancer is “responsive” to a therapeutic agent if its rate of growth is inhibited as a result of contact with the therapeutic agent, compared to its growth in the absence of contact with the therapeutic agent. Growth of a cancer can be measured in a variety of ways, for instance, the size of a tumor or the expression of tumor markers appropriate for that tumor type may be measured. 
     A cancer is “non-responsive” to a therapeutic agent if its rate of growth is not inhibited, or inhibited to a very low degree, as a result of contact with the therapeutic agent when compared to its growth in the absence of contact with the therapeutic agent. As stated above, growth of a cancer can be measured in a variety of ways, for instance, the size of a tumor or the expression of tumor markers appropriate for that tumor type may be measured. The quality of being non-responsive to a therapeutic agent is a highly variable one, with different cancers exhibiting different levels of “non-responsiveness” to a given therapeutic agent, under different conditions. Still further, measures of non-responsiveness can be assessed using additional criteria beyond growth size of a tumor, including patient quality of life, degree of metastases, etc. 
     An application of this test will predict end points including, but not limited to, overall survival, progression free survival, radiological response, as defined by RECIST, complete response, partial response, stable disease and serological markers such as, but not limited to, PSA, CEA, CA125, CA15-3 and CA19-9. In other embodiments of this invention it can be used to evaluate endoscopic ultrasound, CT, spiral CT, FDG-PET, pathologic, histological response in oesophageal cancer treated with DNA damaging combination therapies, alone or in the context of standard treatment. 
     Array or non-array based methods for detection, quantification and qualification of RNA, DNA or protein within a sample of one or more nucleic acids or their biological derivatives such as encoded proteins may be employed, including quantitative PCR (QPCR), enzyme-linked immunosorbent assay (ELISA) or immunohistochemistry (IHC) and the like. 
     After obtaining an expression profile from a sample being assayed, the expression profile is compared with a reference or control profile to make a diagnosis regarding the therapy responsive phenotype of the cell or tissue, and therefore host, from which the sample was obtained. The terms “reference” and “control” as used herein in relation to an expression profile mean a standardized pattern of gene or gene product expression or levels of expression of certain biomarkers to be used to interpret the expression classifier of a given patient and assign a prognostic or predictive class. The reference or control expression profile may be a profile that is obtained from a sample known to have the desired phenotype, e.g., responsive phenotype, and therefore may be a positive reference or control profile. In addition, the reference profile may be from a sample known to not have the desired phenotype, and therefore be a negative reference profile. 
     If quantitative PCR is employed as the method of quantitating the levels of one or more nucleic acids, this method may quantify the PCR product accumulation through measurement of fluorescence released by a dual-labeled fluorogenic probe (e.g. a TaqMan® probe or a molecular beacon or FRET/Light Cycler probes). Some methods may not require a separate probe, such as the Scorpion and Ampliflyor systems where the probes are built into the primers. 
     In certain embodiments, the obtained expression profile is compared to a single reference profile to obtain information regarding the phenotype of the sample being assayed. In yet other embodiments, the obtained expression profile is compared to two or more different reference profiles to obtain more in depth information regarding the phenotype of the assayed sample. For example, the obtained expression profile may be compared to a positive and negative reference profile to obtain confirmed information regarding whether the sample has the phenotype of interest. 
     The comparison of the obtained expression profile and the one or more reference profiles may be performed using any convenient methodology, where a variety of methodologies are known to those of skill in the array art, e.g., by comparing digital images of the expression profiles, by comparing databases of expression data, etc. Patents describing ways of comparing expression profiles include, but are not limited to, U.S. Pat. Nos. 6,308,170 and 6,228,575, the disclosures of which are herein incorporated by reference. Methods of comparing expression profiles are also described above. 
     The comparison step results in information regarding how similar or dissimilar the obtained expression profile is to the one or more reference profiles, which similarity information is employed to determine the phenotype of the sample being assayed. For example, similarity with a positive control indicates that the assayed sample has a responsive phenotype similar to the responsive reference sample. Likewise, similarity with a negative control indicates that the assayed sample has a non-responsive phenotype to the non-responsive reference sample. 
     The level of expression of a biomarker can be further compared to different reference expression levels. For example, a reference expression level can be a predetermined standard reference level of expression in order to evaluate if expression of a biomarker or biomarker set is informative and make an assessment for determining whether the patient is responsive or non-responsive. Additionally, determining the level of expression of a biomarker can be compared to an internal reference marker level of expression which is measured at the same time as the biomarker in order to make an assessment for determining whether the patient is responsive or non-responsive. For example, expression of a distinct marker panel which is not comprised of biomarkers of the invention, but which is known to demonstrate a constant expression level can be assessed as an internal reference marker level, and the level of the biomarker expression is determined as compared to the reference. In an alternative example, expression of the selected biomarkers in a tissue sample which is a non-tumor sample can be assessed as an internal reference marker level. The level of expression of a biomarker may be determined as having increased expression in certain aspects. The level of expression of a biomarker may be determined as having decreased expression in other aspects. The level of expression may be determined as no informative change in expression as compared to a reference level. In still other aspects, the level of expression is determined against a pre-determined standard expression level as determined by the methods provided herein. 
     The invention is also related to guiding conventional treatment of patients. Patients in which the diagnostics test reveals that they are responders to the drugs, of the classes that directly or indirectly affect DNA damage and/or DNA damage repair, can be administered with that therapy and both patient and oncologist can be confident that the patient will benefit. Patients that are designated non-responders by the diagnostic test can be identified for alternative therapies which are more likely to offer benefit to them. 
     The invention further relates to selecting patients for clinical trials where novel drugs of the classes that directly or indirectly affect DNA damage and/or DNA damage repair in order to treat OAC. Enrichment of trial populations with potential responders will facilitate a more thorough evaluation of that drug under relevant criteria. 
     The invention still further relates to methods of diagnosing patients as having or being susceptible to developing OAC associated with a DNA damage response deficiency (DDRD). DDRD is defined herein as any condition wherein a cell or cells of the patient have a reduced ability to repair DNA damage, which reduced ability is a causative factor in the development or growth of a tumor. The DDRD diagnosis may be associated with a mutation in the Fanconi anemia/BRCA pathway. The DDRD diagnosis may also be associated with adenocarcinoma. The methods of diagnosing an individual having oesophageal adenocarcinoma (OAC) may comprise:
         a. measuring expression levels of one or more biomarkers in a test sample obtained from the individual, wherein the one or more biomarkers are selected from Table 1A, 1B, 2A, 2B, 3 or 4;   b. deriving a test score that captures the expression levels;   c. providing a threshold score comprising information correlating the test score and diagnosis of OAC;   d. and comparing the test score to the threshold score; wherein the individual is determined to have OAC or be susceptible to developing OAC when the test score exceeds the threshold score.       

     The methods of diagnosis may comprise the steps of obtaining a test sample from the individual; measuring expression levels of one or more biomarkers in the test sample, wherein the one or more biomarkers are selected from the group consisting of CXCL10, MX1, IDO1, IF144L, CD2, GBP5, PRAME, ITGAL, LRP4, and APOL3; deriving a test score that captures the expression levels; providing a threshold score comprising information correlating the test score and a diagnosis of the OAC; and comparing the test score to the threshold score; wherein the individual is determined to have the cancer or is susceptible to developing the cancer when the test score exceeds the threshold score. One of ordinary skill in the art can determine an appropriate threshold score, and appropriate biomarker weightings, using the teachings provided herein including the teachings of Example 1. 
     In other embodiments, the methods of diagnosing patients as having or being susceptible to developing OAC associated with DDRD comprise measuring expression levels of one or more biomarkers in the test sample, wherein the one or more biomarkers are selected from the group consisting of CXCL10, MX1, IDO1, IF144L, CD2, GBP5, PRAME, ITGAL, LRP4, APOL3, CDR1, FYB, TSPAN7, RAC2, KLHDC7B, GRB14, AC138128.1, KIF26A, CD274, CD109, ETV7, MFAP5, OLFM4, PI15, FOSB, FAM19A5, NLRC5, PRICKLE1, EGR1, CLDN10, ADAMTS4, SP140L, ANXA1, RSAD2, ESR1, IKZF3, OR2I1P, EGFR, NAT1, LATS2, CYP2B6, PTPRC, PPP1R1A, and AL137218.1. Tables 2A and 2B provide exemplary gene signatures (or gene classifiers) wherein the biomarkers consist of 40 or 44 of the gene products listed therein, respectively, and wherein a threshold score is derived from the individual gene product weightings listed therein. In one of these embodiments wherein the biomarkers consist of the 44 gene products listed in Table 2B, and the biomarkers are associated with the weightings provided in Table 2B, a test score that exceeds a threshold score, such as a threshold score of 0.3681, indicates a diagnosis of OAC or of being susceptible to developing OAC. 
    
    
     
       DESCRIPTION OF THE FIGURES 
         FIG. 1 : Kaplan Meier plot for DDRD signature predicting relapse-free survival (RFS) in EAC patients treated with neo-adjuvant chemotherapy. 
         FIG. 2 : Kaplan Meier for DDRD signature predicting overall survival (PFS) in EAC patients treated with neo-adjuvant chemotherapy. 
         FIG. 3 : Kaplan Meier for DDRD signature predicting survival (PFS) in EAC patients not treated with neo-adjuvant chemotherapy. 
     
    
    
     The following examples are offered by way of illustration and not by way of limitation. 
     EXAMPLES 
     Example 1 
     Application of DDRD Assay to Oesophageal Adenocarcinoma (OAC) and Validation 
     Methods 
     Samples 
     46 FFPE pre-chemotherapy (platinum-based; cisplatin treatment) oesophageal endoscopy biopsy samples with appropriate ethical approval and consent information for the use of human tissue were used to demonstrate the predictive ability of the DDRD assay (based on the 44 genes listed in table 2B) in Oesophageal cancer (OAC). Five FFPE tissue slides were received per sample along with a “marked up” Haematoxylin &amp; Eosin (H&amp;E) stained slide per sample. H&amp;E slides were reviewed for pathology subtypes and marked for macrodissection. 
     Gene Expression Profiling 
     All samples were processed in a quality controlled environment according to the appropriate Standard Operating Procedures (SOPs). 
     All 5 sections per sample were macrodissected, and extracted using the Ambion RecoverAll kit. The lysate was split in two and for most cases only one half of the material was sufficient for profiling. In 2 cases both aliquots of the macrodissected material was extracted Samples were randomised and extracted over 3 batches. 
     Following extraction sample concentration was determined using the Nanodrop 1000 spectrophotometer. Vacuum concentration was required for 5 samples to yield a suitable concentration of RNA for cDNA amplification. 
     All 36 RNA samples, whether these were the original primary extract, vacuum concentrated or the secondary re-extract, advanced to amplification using the NUGEN V3 FFPE Amplification kit. The 46 samples were profiled using the Ovation® FFPE WTA System. Following quality control of the generated cDNA using both the spectrophotometer and the Agilent 2100 Bioanalyzer, the samples were fragmented and labelled using the NuGEN Encore Biotin module. Following the fragmentation QC, the fragmented and labelled product was then hybridized to Xcel arrays in accordance with the NuGEN guidelines for hybridisation onto Affymetrix GeneChip® arrays. 
     Array QC 
     Following microarray profiling a quality control (QC) assessment of the data was performed. The sample and array quality was assessed using Almac&#39;s proprietary toolbox. One of the forty-six samples failed microarray QC. This sample failed on image analysis, present call, scaling factor and distribution. Therefore 45 samples were used in subsequent analysis. 
     Data Preparation 
     Samples were pre-processed using Almac&#39;s pre-processing toolbox, with RMA settings (background correction; quantile normalisation using the median for average; and median polish summarisation). A cross mapping of probesets representing the DDRD genes was used to extract relevant probesets for analysis. 
     Calculating Classifier Scores 
     The probe sets that map to the genes in the classifier are determined, excluding anti-sense probe sets (if applicable). The median intensity over all probe sets relating to each gene in the classifier is calculated resulting in a reduced gene intensity matrix. If no probe sets exist for the gene on the particular array platform, the observed average from the training data will be used as a replacement. The median value of all probe sets for each sample is calculated and subtracted from the reduced gene intensity matrix. 
     The classifier score of a sample is calculated as a weighted sum of the expression of genes in the signature. 
     
       
         
           
             SignatureScore 
             = 
             
               
                 
                   ∑ 
                   i 
                 
                  
                 
                   
                     w 
                     i 
                   
                   × 
                   
                     ( 
                     
                       
                         ge 
                         i 
                       
                       - 
                       
                         b 
                         i 
                       
                       - 
                       
                         a 
                         median 
                       
                     
                     ) 
                   
                 
               
               + 
               k 
             
           
         
       
     
     where w i  is a weight for each gene, b i  is a gene-specific bias which is required to mean center each gene relative to the training data (Table 4), a median  is the median expression intensity across all probe sets on the array, ge i  is the observed gene expression level after pre-processing, and k=0.3738 is a constant offset. 
     The classifier score is calculated for each sample, which can then be used to stratify patients from, for example, more likely to respond to less likely to respond. 
     Results 
       
     
       
         
           
               
             
               
                 TABLE 5 
               
             
            
               
                   
               
               
                 Multivariable analysis of recurrence free survival  
               
               
                 (43 samples, 20 non-events and 23 events) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Hazard  
                 Lower  
                 Upper 
                   
               
               
                   
                 Ratio 
                 95% CI 
                 95% CI 
                 P-value 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 DDRD-positive 
                 0.069 
                 0.007 
                 0.613 
                 0.0189 
               
               
                 N Stage 1 vs 0 
                 3.173 
                 0.933 
                 10.783 
                 0.0644 
               
               
                 N Stage 2&amp;3 vs 0 
                 7.089 
                 2.092 
                 24.023 
                 0.0017 
               
               
                 Surgical T Stage 2 vs 0&amp;1 
                 0.249 
                 0.020 
                 3.161 
                 0.2838 
               
               
                 Surgical T Stage 3+ vs 0&amp;1 
                 0.995 
                 0.120 
                 8.273 
                 0.9962 
               
               
                   
               
            
           
         
       
     
     Using Cox proportional hazards regression, there is a statistically significant effect of the DDRD assay on recurrence free survival. The effect is statistically significant when other important risk factors are included in the model (hazard ratio 0.07, 95% CI (0.007, 0.643), p=0.019). Covariates included in the model were directed by clinical expertise. 
     
       
         
           
               
             
               
                 TABLE 6 
               
             
            
               
                   
               
               
                 Multivariable analysis of overall survival  
               
               
                 (43 samples, 17 non-events and 26 events) 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Hazard 
                 Lower 
                 Upper 
                   
               
               
                   
                 Ratio 
                 95% CI 
                 95% CI 
                 P-value 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 DDRD-positive 
                 0.110 
                 0.014 
                 0.885 
                 0.0380 
               
               
                 N Stage 1 vs 0 
                 3.276 
                 0.938 
                 11.450 
                 0.0630 
               
               
                 N Stage 2&amp;3 vs 0 
                 6.991 
                 2.212 
                 22.087 
                 0.0009 
               
               
                 Surgical T Stage 2 vs 0&amp;1 
                 0.412 
                 0.035 
                 4.802 
                 0.4787 
               
               
                 Surgical T Stage 3+ vs 0&amp;1 
                 1.147 
                 0.141 
                 9.309 
                 0.8981 
               
               
                   
               
            
           
         
       
     
     Using Cox proportional hazards regression, there is a statistically significant effect of the DDRD assay on overall survival. The effect is statistically significant when other important risk factors are included in the model (hazard ratio 0.11, 95% CI (0.014, 0.885), p=0.038). Covariates included in the model were directed by clinical expertise. 
     Conclusion 
     Evidence is provided demonstrating that the DDRD assay, is a significant prognosticator of recurrence free survival (p=0.019) and overall survival (p=0.038) in oesophageal cancer after adjusting for other important covariates. 
     Example 2 
     Background 
     Oesophageal cancer is the eighth most common cancer worldwide with 480,000 cases diagnosed annually. In the UK the incidence of the disease in men has risen 68% in the last 45 years with the most common pathological subtype being adenocarcinoma. The reason for this increase in oesophageal adenocarcinoma (OAC) is unclear but development of this tumour is strongly associated with the pre-malignant metaplastic condition, Barrett&#39;s oesophagus. This is caused by gastro-oesophageal reflux disease and, along with obesity, smoking and alcohol intake, is one of the strongest risk factors for OAC. Despite efforts to screen for Barrett&#39;s oesophagus and pre-operatively select OAC patients who are likely to benefit from potentially curative surgery, survival remains poor. The five year survival rate is 13% and even in early stage loco-regional confined disease this figure rarely exceeds 40%. A significant improvement in overall survival has been demonstrated with neo-adjuvant or peri-operative therapy but the optimal approach for individual patients remains unclear. There is a pressing need to identify biomarkers capable of predicting response, enabling clinicians to stratify patients to the neo-adjuvant therapy which would be most beneficial. 
     Oesophageal Adenocarcinoma—Patient Cohort 
     Considering one of the most active drugs in OAC is the DNA-damaging agent Cisplatin we decided to investigate the efficacy of the DDRD signature in OAC. We analyzed 63 formalin fixed paraffin embedded (FFPE) pre-treatment endoscopic biopsy specimens from early stage OAC patients treated with cisplatin-based neo-adjuvant chemotherapy followed by surgical resection between 2004 and 2010 at the Northern Ireland Cancer Centre (Tables 7 and 8). 
     The 63 samples used in this example include the 45 samples from Example 1. We profiled an additional small sample set and merged the two sample sets together for this analysis. Accordingly, the methods described in Example 1 are applicable to this example. 
     Biopsies were reviewed for pathological subtype prior to marking for macrodissection and samples containing at least 70% adenocarcinoma tissue were to be taken forward. The matched resection specimens were scored according to the Mandard Score (&lt;3=pathological response). A sufficient quantity of RNA was extracted from 62 out of the 63 FFPE biopsy specimens (98.4%) and hybridized to the Xcel™ array, a cDNA microarray-based technology optimized for archival FFPE tissue (Almac/Affymetrix). All samples were scored for the DDRD assay. 
     
       
         
           
               
             
               
                 TABLE 7 
               
             
            
               
                   
               
               
                 Patient Characteristics. 
               
            
           
           
               
               
               
            
               
                   
                 Patient Characteristics 
                 n = 62 
               
               
                   
                   
               
               
                   
                 Sex, n (%) 
                   
               
               
                   
                 Male 
                 49 (79) 
               
               
                   
                 Female 
                 13 (21) 
               
               
                   
                 Age, years (%) 
                   
               
               
                   
                 &lt;60 
                 21 (33.9) 
               
               
                   
                 60-69 
                 29 (46.8) 
               
               
                   
                 ≧70 
                 12 (19.4) 
               
               
                   
                 Median 
                 62 
               
               
                   
                 Range 
                 39-78 
               
               
                   
                 ECOG Performance Status, n (%) 
                   
               
               
                   
                 0 
                  6 (9.7) 
               
               
                   
                 1 
                 52 (83.9) 
               
               
                   
                 2 
                  4 (6.5) 
               
               
                   
                 Primary Site, n (%) 
                   
               
               
                   
                 Oesophagus 
                  5 (8.1) 
               
               
                   
                 GOJ, Siewert 1 
                 43 (69.4) 
               
               
                   
                 GOJ, Siewert 2 
                 10 (16.1) 
               
               
                   
                 GOJ, Siewert 3 
                  4 (6.5) 
               
               
                   
                 Chemotherapy treatment, n (%) 
                   
               
               
                   
                 ECF/X 
                 59 (95.2) 
               
               
                   
                 ECarboX 
                  2 (3.2) 
               
               
                   
                 CFU 
                  1 (1.6) 
               
               
                   
                 PET Response, n (%) 
                   
               
               
                   
                 Responder 
                 15 (24.2) 
               
               
                   
                 Non-responder 
                 33 (53.2) 
               
               
                   
                 Unknown 
                 14 (22.6) 
               
               
                   
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 8 
               
             
            
               
                   
               
               
                 Tumour Characteristics. 
               
            
           
           
               
               
               
            
               
                   
                 Tumour Characteristics 
                 n = 61* 
               
               
                   
                   
               
               
                   
                 Tumour stage, n (%) 
                   
               
               
                   
                 T1 
                  9 (14.8) 
               
               
                   
                 T2 
                 11 (18) 
               
               
                   
                 T3 
                 39 (63.9) 
               
               
                   
                 T4 
                  2 (3.3) 
               
               
                   
                 Nodal Status, n (%) 
                   
               
               
                   
                 N0 
                 20 (32.8) 
               
               
                   
                 N1 
                 16 (26.2) 
               
               
                   
                 N2 
                 13 (21.3) 
               
               
                   
                 N3 
                 12 (19.7) 
               
               
                   
                 Differentiation, n (%) 
                   
               
               
                   
                 Well 
                  4 (6.6) 
               
               
                   
                 Moderate 
                 24 (39.3) 
               
               
                   
                 Moderate/poor 
                  6 (9.8) 
               
               
                   
                 Poor 
                 27 (44.3) 
               
               
                   
                 Lymphovascular invasion, n (%) 
                   
               
               
                   
                 Present 
                 37 (60.7) 
               
               
                   
                 Absent 
                 22 (36.1) 
               
               
                   
                 Unknown 
                  2 (3.3) 
               
               
                   
                 Distal Margin involved, n (%) 
                   
               
               
                   
                 Yes 
                  1 (1.6) 
               
               
                   
                 No 
                 60 (98.4) 
               
               
                   
                 Circumferential Margin involved, n (%) 
                   
               
               
                   
                 Yes 
                 33(54.1) 
               
               
                   
                 No 
                 28 (45.9) 
               
               
                   
                   
               
               
                   
                 *One pathology report unable to be retrieved 
               
            
           
         
       
     
     The association between the DDRD score and prognosis was assessed by Kaplan-Meier analysis and Cox Proportional Hazards regression. A total of 13 samples (21%) were characterised as DDRD positive with the remaining 49 samples (79%) DDRD negative. DDRD assay positivity demonstrated a statistically significant association with relapse-free survival (RFS) (HR 0.33; 95% CI 0.13-0.87; p=0.024) resulting in a median RFS of 65.2 months for DDRD+ve patients vs 33.9 months for DDRD-ve patients ( FIG. 1 ). Following multivariable analysis the effect of the DDRD assay on RFS remained statistically significant when other important risk factors were included in the model (HR 0.31, 95% CI (0.110, 0.877), p=0.027) (Table 9). Median overall survival (OS) was significantly higher in DDRD+ve patients (94.3 months vs 32.2 months; HR 0.32; 95% CI 0.12-0.82; p=0.017) ( FIG. 2 ). Following multivariable analysis the effect of the DDRD assay on OS remained statistically significant when other important risk factors were included in the model (HR 0.36, 95% CI (0.132, 0.953), p=0.040). These results indicate that the DDRD assay is a strong prognostic marker in the setting of neo-adjuvant chemotherapy for early stage esophageal adenocarcinoma. 
     
       
         
           
               
             
               
                 TABLE 9 
               
             
            
               
                   
               
               
                 Multivariable analysis of the predictive value of the DDRD  
               
               
                 assay for relapse-free survival 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Hazard 
                 Lower  
                 Upper  
                   
               
               
                   
                 Ratio 
                 95% CI 
                 95% CI 
                 P-value 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 DDRD-positive 
                 0.31 
                 0.11 
                 0.877 
                 0.027 
               
               
                 Surgical T stage 2 vs 0/1 
                 0.343 
                 0.078 
                 1.519 
                 0.159 
               
               
                 Surgical T stage 3+ vs 0/1 
                 0.47 
                 0.126 
                 1.751 
                 0.261 
               
               
                 N stage 1 vs 0 
                 4.793 
                 1.59 
                 14.447 
                 0.005 
               
               
                 N stage 2&amp;3 vs 0 
                 7.822 
                 2.777 
                 22.034 
                 &lt;0.001 
               
               
                   
               
            
           
         
       
     
     
       
         
           
               
             
               
                 TABLE 10 
               
             
            
               
                   
               
               
                 Multivariable analysis of the predictive value of the DDRD  
               
               
                 assay for overall survival 
               
            
           
           
               
               
               
               
               
            
               
                   
                 Hazard 
                 Lower 
                 Upper 
                   
               
               
                   
                 Ratio 
                 95% CI 
                 95% CI 
                 P-value 
               
               
                   
               
            
           
           
               
               
               
               
               
            
               
                 DDRD-positive 
                 0.355 
                 0.132 
                 0.953 
                 0.040 
               
               
                 Surgical T stage 2 vs 0/1 
                 0.402 
                 0.098 
                 1.644 
                 0.204 
               
               
                 Surgical T stage 3+ vs 0/1 
                 0.411 
                 0.112 
                 1.514 
                 0.181 
               
               
                 N stage 1 vs 0 
                 6.679 
                 2.090 
                 21.341 
                 0.001 
               
               
                 N stage 2&amp;3 vs 0 
                 8.809 
                 3.031 
                 25.605 
                 &lt;0.001 
               
               
                   
               
            
           
         
       
     
     DDRD in OAC—Chemotherapy Naive 
     To determine whether the DDRD assay was prognostic independent of treatment it was applied to a dataset of 75 fresh frozen tissue samples derived from potentially curative resections for oesophageal and gastro-oesophageal junction tumours. These resections were carried out between 1992 and 200 and none of the patients received neo-adjuvant chemotherapy. All samples were analysed using a custom-made Agilent 44K 60-mer oligo-microarray. As shown in  FIG. 3 , no difference in survival was noted between the DDRD positive and negative populations HR 0.64 (95% CI 0.17-2.43). Further Affymetrix-based validation sets are being sought in order to provide a consistent analysis platform between datasets. 
     CONCLUSIONS 
     
         
         
           
             The DDRD assay could personalise the treatment approach and improve outcomes for early stage EAC patients. 
             First array-based biomarker to be developed from FFPE tissue in EAC. 
             Transcriptional profiling successful for 98% of pre-treatment FFPE endoscopic biopsies. 
             The DDRD assay demonstrates a strong association with prognosis. 
             A positive DDRD assay result predicted increased RFS (HR 0.31, 95% CI (0.11-0.88), p=0.027) and OS (HR 0.36, 95% CI (0.13-0.95), p=0.04) compared with the assay negative population. 
             DDRD positive patients have a five-year survival of 59% compared to 17% for DDRD negative patients. 
             Expansion of this validation set is ongoing to increase the statistical power of the analysis. 
             The role of the DDRD assay in early stage EAC will be assessed in a prospective clinical trial. 
           
         
       
    
     The various embodiments of the present invention are not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the various embodiments of the invention in addition to those described herein will become apparent to those skilled in the art from the foregoing description and accompanying figures. Such modifications are intended to fall within the scope of the appended claims. Moreover, all embodiments described herein are considered to be broadly applicable and combinable with any and all other consistent embodiments, as appropriate. 
     Various publications are cited herein, the disclosures of which are incorporated by reference in their entireties.