Patent Publication Number: US-2022235304-A1

Title: Bioprocessing system and tubing and component management apparatus for a bioprocessing system

Description:
BACKGROUND 
     Technical Field 
     Embodiments of the invention relate generally to bioprocessing systems and methods and, more particularly, to a tubing and component management system for a bioprocessing system. 
     Discussion of Art 
     A variety of vessels, devices, components and unit operations are known for carrying out biochemical and/or biological processes and/or manipulating liquids and other products of such processes. In order to avoid the time, expense, and difficulties associated with sterilizing the vessels used in biopharmaceutical manufacturing processes, single-use or disposable bioreactor bags and single-use mixer bags are used as such vessels. For instance, biological materials (e.g., animal and plant cells) including, for example, mammalian, plant or insect cells and microbial cultures can be processed using disposable or single-use mixers and bioreactors. 
     Increasingly, in the biopharmaceutical industry, single use or disposable containers are used. Such containers can be flexible or collapsible plastic bags that are supported by an outer rigid structure such as a stainless steel shell or vessel. Use of sterilized disposable bags eliminates time-consuming step of cleaning of the vessel and reduces the chance of contamination. The bag may be positioned within the rigid vessel and filled with the desired fluid for mixing. Depending on the fluid being processed, the system may include a number of fluid lines and different sensors, probes and ports coupled with the bag for monitoring, analytics, sampling, and fluid transfer. For example, a plurality of ports may typically be located at the front of the bag and accessible through an opening in the sidewall of the vessel, which provide connection points for sensors, probes and/or fluid sampling lines. In addition, a harvest port or drain line fitting is typically located at the bottom of the disposable bag and is configured for insertion through an opening in the bottom of the vessel, allowing for a harvest line to be connected to the bag for harvesting and draining of the bag after the bioprocess is complete. 
     Typically, an agitator assembly disposed within the bag is used to mix the fluid. Existing agitators are either top-driven (having a shaft that extends downwardly into the bag, on which one or more impellers are mounted) or bottom-driven (having an impeller disposed in the bottom of the bag that is driven by a magnetic drive system or motor positioned outside the bag and/or vessel). Most magnetic agitator systems include a rotating magnetic drive head outside of the bag and a rotating magnetic agitator (also referred to in this context as the “impeller”) within the bag. The movement of the magnetic drive head enables torque transfer and thus rotation of the magnetic agitator allowing the agitator to mix a fluid within the vessel. Magnetic coupling of the agitator inside the bag, to a drive system or motor external to the bag and/or bioreactor vessel, can eliminate contamination issues, allow for a completely enclosed system, and prevent leakage. Because there is no need to have a drive shaft penetrate the bioreactor vessel wall to mechanically spin the agitator, magnetically coupled systems can also eliminate the need for having seals between the drive shaft and the vessel. 
     Installation and setup of the flexible bioprocessing bag within the bioreactor vessel, along with the associated tubing, filter heaters, impeller and other components can be a labor intensive and time-consuming process. For example, existing bioreactor vessels may present accessibility issues, making it difficult to align and properly seat the impeller with the bioreactor vessel base. Multiple operators and ladders may also be needed, especially for the installation of tubing and filter heaters, which are located at the top of the vessel. Moreover, lack of tubing support for the various tubes connected to the flexible bag can lead to a cluttered array of tubes around the bioreactor vessel. In addition to the above, with existing systems, inflation and deflation of the flexible bioprocessing bag consumable can also a time-consuming process, taking between 10 minutes and almost an hour. 
     In addition to difficulties installing the flexible bioprocessing bag and other components at the top of the bioreactor vessel, properly seating the impeller base plate of the flexible bioprocessing bag on the bottom of the bioreactor vessel during installation of the flexible bag may also present challenges. In particular, with existing systems, there is no feedback mechanism, other than visual inspection, to indicate that the impeller base plate of the flexible bag is properly seated within the recess in the bottom of the bioreactor vessel. Even when a visual inspection reveals that the base plate is properly seated, movement of the base plate before mating of the agitator and magnetic drive assembly beneath the vessel is possible. 
     In view of the above, there is a need for a tubing and component management system for a bioprocessing system that is ergonomically efficient, facilitates installation and setup, and/or assists in the inflation and deflation of the flexible bioprocessing bag. 
     BRIEF DESCRIPTION 
     In an embodiment, a bioreactor vessel includes a bottom, a peripheral sidewall, the bottom and the peripheral sidewall defining an interior space for receiving a flexible bioprocessing bag, a recess in the bottom for receiving a base plate of the flexible bioprocessing bag, and a locking mechanism configured to retain the base plate in the recess. 
     In another embodiment, a bioprocessing apparatus includes a flexible bioprocessing bag, and a base plate positioned at a bottom of the flexible bioprocessing bag and being shaped so as to be received in a corresponding recess in a bottom of a bioreactor vessel. The base plate includes a locating mechanism adjacent to a rear edge of the base plate, for cooperating with a corresponding locating feature on the bottom of the bioreactor vessel adjacent to the recess to locate the base plate in the recess, and a locking mechanism extending downwardly from an underside of the base plate opposite the locating mechanism, for cooperating with a corresponding locking device of the bioreactor vessel for retaining the base plate in the recess. 
     In yet another embodiment, a bioprocessing system includes a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, and a locking mechanism adjacent to the recess, and a flexible bioprocessing bag positionable within the interior space, the flexible bioprocessing bag including a base plate at a bottom of the flexible bioprocessing bag. The base plate is configured to be received in the recess in the bottom of the bioreactor vessel. The locking mechanism is configured to engage the base plate to retain the base plate in the recess. In an embodiment, the locking mechanism includes a latch, and the base plate includes a catch. The latch is moveable between an engagement position where the latch engages the catch when the base plate is positioned in the recess to retain the base plate in the recess, and a clearance position where the base plate can be withdrawn from the recess. 
     In yet a further embodiment, a bioprocessing system includes a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, a locking mechanism adjacent to the recess, an indicator mechanism, and a flexible bioprocessing bag positionable within the interior space, the flexible bioprocessing bag including a base plate at a bottom of the flexible bioprocessing bag. The base plate is configured to be received in the recess in the bottom of the bioreactor vessel. The locking mechanism is configured to engage the base plate to retain the base plate in the recess. In an embodiment, the locking mechanism includes a latch, and the base plate includes a catch. The latch is moveable between an engagement position where the latch engages the catch when the base plate is positioned in the recess to retain the base plate in the recess, and a clearance position where the base plate can be withdrawn from the recess. The indicator mechanism is configured indicate when the base plate is properly position within the recess. In embodiments, the indicator mechanism includes a plunger, a rocker arm, and an indicator. The plunger is configured to be pressed down when the base plate is properly positioned within the recess, the movement of which translates, via the rocker arm, into movement of the indicator. In further embodiments, the rocker arm is configured to move from a position in which it prevents the locking mechanism from engaging the base plate to a position in which the locking mechanism is free to engage the base plate, corresponding to an improper and proper position of the base plate within the recess. In still further embodiments, the indicator mechanism includes at least one sensor configured to indicate when the base plate is properly position within the recess. 
     In an embodiment, a bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel and providing access to the interior space, and a tubing and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting of at least one consumable component of the bioprocessing system. The mounting frame is moveable vertically into and out of the interior space. 
     In another embodiment, a method for installing components of a bioprocessing system includes lowering a mounting frame into a vessel through a top opening of the vessel, opening an access door in a sidewall of the vessel to access the mounting frame, mounting at least one consumable component to the mounting frame, closing the access door, and raising the mounting frame to a position adjacent to a top of the vessel. 
     In yet another embodiment, a bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel and providing access to the interior space through an access door opening, and a tubing and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting of at least one consumable component of the bioprocessing system. The mounting frame is moveable between and installation position where the mounting frame is positioned within the interior space of the vessel at a height where the mounting frame is accessible through the access door, and an operational position where the mounting frame is positioned generally above a top of the bioreactor vessel. The tubing and component management apparatus includes a lift mechanism for moving the mounting frame between the installation position and the operational position. 
    
    
     
       DRAWINGS 
       The present invention will be better understood from reading the following description of non-limiting embodiments, with reference to the attached drawings, wherein below: 
         FIG. 1  is a perspective view of a bioreactor system according to an embodiment of the invention. 
         FIG. 2  is another perspective view of the bioreactor system of  FIG. 1 , illustrating an access door in a closed position. 
         FIG. 3  is another perspective view of the bioreactor system of  FIG. 1 , illustrating the access door in an open position. 
         FIG. 4  is a perspective view of a bioreactor system according to another embodiment of the invention. 
         FIG. 5  is perspective view of the bioreactor system of  FIG. 4 , illustrating an access position of a tubing and component management apparatus. 
         FIG. 6  is a perspective view of the bioreactor system of  FIG. 4 , illustrating a loading position of the tubing and component management apparatus. 
         FIG. 7  is a perspective view of the bioreactor system of  FIG. 4 , illustrating consumable components installed on the tubing and component management apparatus. 
         FIG. 8  is a perspective view of the bioreactor system of  FIG. 4 , illustrating a ready position of the tubing and component management apparatus. 
         FIG. 9  is a perspective view of the bioreactor system of  FIG. 4 , illustrating an operational position of the tubing and component management apparatus. 
         FIG. 10  is a perspective view of a tubing and component management apparatus, according to another embodiment of the invention. 
         FIG. 11  is an enlarged, detail view of the tubing and component management apparatus of  FIG. 10 . 
         FIG. 12  is another enlarged, detail view of the tubing and component management apparatus of  FIG. 10 . 
         FIG. 13  is yet another enlarged, detail view of the tubing and component management apparatus of  FIG. 10 . 
         FIG. 14  is a top plan view of a bioreactor vessel with which the tubing and component management apparatus of  FIG. 10  may be utilized. 
         FIG. 15  is perspective view of a bioreactor system according to another embodiment of the invention. 
         FIG. 16  is a perspective view of the bioreactor system of  FIG. 5 , illustrating a loading position of the tubing and component management apparatus. 
         FIG. 17  is a perspective view of the bioreactor system of  FIG. 5 , illustrating consumable components installed on the tubing and component management apparatus. 
         FIG. 18  is a perspective view of the bioreactor system of  FIG. 5 , illustrating a ready position of the tubing and component management apparatus. 
         FIG. 19  is a perspective view of the bioreactor system of  FIG. 5 , illustrating an operational position of the tubing and component management apparatus. 
         FIG. 20  is a perspective view of a base plate of a flexible bioprocessing bag, for use with a bioreactor system, according to an embodiment of the invention. 
         FIG. 21  is a top perspective view of a locking system for a base plate of a bioreactor system, according to another embodiment of the invention. 
         FIG. 22  is a bottom perspective view of a locking system of  FIG. 21 . 
         FIG. 23  is a side elevational view of the locking system of  FIG. 21 . 
         FIG. 24  is a cross-sectional, perspective view of a base plate locked in position within a bioreactor vessel using the locking system of  FIG. 21 . 
         FIG. 25  is a side cross-sectional view of the base plate locked in position within a bioreactor vessel using the locking system of  FIG. 21 . 
         FIG. 26  is a top perspective view of a locking and indicator system for a base plate of a bioreactor system, according to another embodiment of the invention. 
         FIG. 27  is a bottom perspective view of the locking and indicator system of  FIG. 26 . 
         FIG. 28  is a side view of the locking and indicator system of  FIG. 26  when the baseplate is improperly positioned. 
         FIG. 29  is a side view of the locking and indicator system of  FIG. 26  when the baseplate is properly positioned. 
     
    
    
     DETAILED DESCRIPTION 
     Reference will be made below in detail to exemplary embodiments of the invention, examples of which are illustrated in the accompanying drawings. Wherever possible, the same reference characters used throughout the drawings refer to the same or like parts. 
     As used herein, the term “flexible” or “collapsible” refers to a structure or material that is pliable, or capable of being bent without breaking, and may also refer to a material that is compressible or expandable. An example of a flexible structure is a bag formed of polyethylene film. The terms “rigid” and “semi-rigid” are used herein interchangeably to describe structures that are “non-collapsible,” that is to say structures that do not fold, collapse, or otherwise deform under normal forces to substantially reduce their elongate dimension. Depending on the context, “semi-rigid” can also denote a structure that is more flexible than a “rigid” element, e.g., a bendable tube or conduit, but still one that does not collapse longitudinally under normal conditions and forces. 
     A “vessel,” as the term is used herein, means a flexible bag, a flexible container, a semi-rigid container, a rigid container, or a flexible or semi-rigid tubing, as the case may be. The term “vessel” as used herein is intended to encompass bioreactor vessels having a wall or a portion of a wall that is flexible or semi-rigid, single use flexible bags, as well as other containers or conduits commonly used in biological or biochemical processing, including, for example, cell culture/purification systems, mixing systems, media/buffer preparation systems, and filtration/purification systems, e.g., chromatography and tangential flow filter systems, and their associated flow paths. As used herein, the term “bag” means a flexible or semi-rigid container or vessel used, for example, as a bioreactor or mixer for the contents within. As used herein, “consumable” or “consumable component” means devices or components that are intended to be replaced regularly due to wear or use. 
     Embodiments of the invention provide bioprocessing systems and, in particular, tubing and components management systems and devices for a bioreactor system. In an embodiment, a bioreactor vessel includes a bottom, a peripheral sidewall, the bottom and the peripheral sidewall defining an interior space for receiving a flexible bioprocessing bag, a recess in the bottom for receiving a base plate of the flexible bioprocessing bag, and a locking mechanism configured to retain the base plate in the recess. 
     Further embodiments of the invention provide bioprocessing systems and, in particular, tubing and components management systems and devices for a bioreactor system. In an embodiment, a bioreactor vessel includes a bottom, a peripheral sidewall, the bottom and the peripheral sidewall defining an interior space for receiving a flexible bioprocessing bag, a recess in the bottom for receiving a base plate of the flexible bioprocessing bag, a locking mechanism configured to retain the base plate in the recess, and an indicator mechanism configured to indicate when the base plate is properly positioned within the recess. 
     Embodiments of the invention provide bioprocessing systems and, in particular, tubing and components management systems and devices for a bioreactor system. In an embodiment, a bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel and providing access to the interior space, and a tubing and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting of at least one consumable component of the bioprocessing system. The mounting frame is moveable vertically into and out of the interior space. 
     With reference to  FIGS. 1-3 , a bioreactor system  10  according to an embodiment of the invention is illustrated. The bioreactor system  10  includes a generally rigid bioreactor vessel or support structure  12  mounted atop a base  14  having a plurality of legs  16 . The vessel  12  may be formed, for example, from stainless steel, polymers, composites, glass, or other metals, and may be cylindrical in shape, although other shapes may also be utilized without departing from the broader aspects of the invention. The vessel  12  can be any shape or size as long as it is capable of supporting a single-use, flexible bioreactor bag in an interior space  18  thereof. For example, according to one embodiment of the invention the vessel  12  is capable of accepting and supporting a 10 L-2000 L flexible or collapsible bioprocess bag. 
     The vessel  12  may include one or more sight windows  20 , which allows an operator to view a fluid level within the flexible bag positioned within the interior space  18 , as well as a window  22  positioned at a lower area of the vessel  12 . The window  22  allows access to the interior of the vessel  12  for insertion and positioning of various sensors and probes (not shown) within the flexible bag, and for connecting one or more fluid lines to the flexible bag for fluids, gases, and the like, to be added or withdrawn from the flexible bag. Sensors/probes and controls for monitoring and controlling important process parameters include any one or more, and combinations of: temperature, pressure, pH, dissolved oxygen (DO), dissolved carbon dioxide (pCO 2 ), mixing rate, and gas flow rate, for example. 
     As best shown in  FIGS. 2 and 3 , in an embodiment, the vessel  12  includes an access door  24  hingedly or pivotally connected to a sidewall of the vessel  12  and moveable between a closed position ( FIG. 2 ) and an open or access position ( FIG. 3 ) permitting access to the interior space  18 . The door  24  may include a handle  26  that facilitates movement of the door between the open and closed positions. In an embodiment, the door  24  may be configured and positioned such that when the door  24  is in the closed position, a lower edge of the door  24  forms an upper edge or boundary of the window  22 , and/or a side edge of the door  24  forms an edge or boundary of the window  20 . By having the edges of the door  24  define one or more boundaries of the windows  22 ,  24 , when the door  22  is in the open position, a contiguous and unobstructed access opening in the sidewall of the vessel is formed by the opening  20 , opening  22  and open door  24  (i.e., the opening in which the door is received). Accordingly, the area of the contiguous access opening formed in the sidewall of the vessel  12  when the door is in the open position is equivalent to the combined areas of the door  24 , window  22  and window  24 . This provides greater clearance and access to the interior space  18  than would otherwise be possible if the door and windows were separated by a portion of the sidewall of the vessel  12 . 
     With further reference to  FIG. 3 , the interior sidewall of the vessel  12  may include one or more vertical baffles  28  that project into the interior space  18 . The baffles  28  may be generally triangular in cross-section, although shapes and configurations known in the art may also be utilized without departing from the broader aspects of the invention. The baffles  28  are configured to contact and bias the flexible bag (when installed in the interior space  18 ) inwardly during a bioprocessing operation, for purposes known in the art. A bottom of the bioreactor vessel  12  includes a locating recess  30  for receiving an impeller base plate, as discussed in detail hereinafter. 
     As further shown in  FIGS. 1-3 , the bioreactor system  10  also includes a tubing and component management apparatus  40 . The apparatus  40  includes a support member  42  mounted to the exterior sidewall of the vessel  12  and extending generally vertically above a top edge of the vessel  12 . In an embodiment, the support member  42  may be mounted to the vessel by way of welding, bolts, screws, clamps or the like, although other means of attachment may also be utilized without departing from broader aspects of the invention. The apparatus  40  further includes a mast arm or boom  44  that extends generally horizontally from a distal end of the support member  42  and over the vessel  12 , a guide sleeve  46  depending downwardly from a distal end of the boom  44 , and a mounting plate  48  having a shaft  50  that is slidably received within the guide sleeve  46 . In an embodiment, the apparatus  40  is configured such that the guide sleeve  46  and the shaft  50  of the mounting plate  50  are aligned with, i.e., coaxial with, a central axis or centerline of the vessel  12 . 
     As best shown in  FIG. 1 , the apparatus  40  also includes a lifting mechanism  52  that is operable to selectively raise and lower the mounting plate  48  within the vessel  12 , as in the directions indicated by arrow, A. For example, in an embodiment, the lifting mechanism may include a cable  54  connected to the shaft  50  of the mounting plate  48 , and which extends through the guide sleeve  46 , along (or within) the boom  44 , and downward along (or within) the support member  42 . A distal end of the cable is connected to an actuator which may be, for example, a hand crank, motor or other driving member. The actuator is operable to selectively retract the cable  54 , thereby raising the mounting plate  48 , or let out the cable  54 , thereby lowering the mounting plate  48  into the vessel  12 . 
     As illustrated in  FIGS. 1-3 , the mounting plate  48  may be generally T-shaped and includes a substantially planar upper surface for the mounting of various components used in bioprocessing operations, such as filters, filter heaters and other consumables. The mounting plate  48  may also include an array of slots or apertures configured to receive and retain various tubes configured for connection to the flexible bag received within the vessel  12 . One or more hooks  56  (or other suitable coupling members) attached to the mounting plate  48  may be utilized to move and/or support the flexible bag. 
     In use, when installing a flexible bioprocessing bag prior to bioprocessing, the access door  24  in the sidewall of the vessel  12  may be opened, allowing for unobstructed access to the interior space  18  within the vessel  12 . The lifting mechanism  52  may then be utilized to lower the mounting plate  48  into the vessel  12  to a height where it is easily accessible to an operator (for example, to about waist-height). At this point, the flexible bioprocessing bag (not shown) can be placed inside the vessel  12  and attached to the hooks  56  of the mounting plate  48 . In addition, various tubes connected to the bag (or to be connected to the bag) can be organized and held out of the way of an operator by routing them through the slots/apertures in the mounting plate  48 . Moreover, various functional components such as filter heaters, filters and other consumables can be attached to the mounting plate  48  such as by bolts. At this point, the actuator of the lifting mechanism  52  may be utilized to raise the mounting plate  48  to an operational position generally at the top of, or above, the vessel  12 . The access door  24  can then be moved to a closed position and a bioprocessing operation commenced. 
     In an embodiment, the lifting mechanism  52  may interface with the control unit of the bioreactor system  10 , such that upon selecting a ‘start’ or ‘inflate’ routine, the lifting mechanism  52  will automatically raise the mounting plate  52  to an operational position at the top of/above the vessel  12 . The position of the mounting plate  48  can also serve as a position stop, limiting the extent to which the bag may be inflated. Similarly, at the end of a bioprocessing operation, selecting a ‘deflate’ or ‘end’ routine may automatically control the lifting mechanism  52  to lower the mounting plate  48 . It is envisioned that, in some embodiments, lowering the mounting plate  48  may assist with deflation of the flexible bioprocessing bag, which has heretofore been a fairly time-consuming process. For example, lowering of the mounting plate  48  onto the bag may exert a downward force on the bag, assisting with deflation. 
     The bioreactor system  10  of the invention therefore provides an ergonomic means of installing the flexible bioprocessing bag, filters, filter heaters, and other consumables, and for organizing the various tubes connected to the bag. In contrast to existing systems, installation of such components can be carried out at waist-height from the side of the bioreactor vessel  12 , obviating the need for multiple operators and stepladders. 
     Turning now to  FIGS. 4-9 , a bioreactor system  100  according to another embodiment of the invention is illustrated. The bioreactor system  100  is generally similar in configuration to the bioreactor system  10  of  FIGS. 1-3  and includes a generally rigid bioreactor vessel or support structure  112  mounted atop a base  114  having a plurality of legs  116 . The vessel  112 , like vessel  12  is capable of supporting a single-use, flexible bioreactor bag in an interior space  118  thereof. The vessel  112  may likewise include one or more sight windows  120  and window  122  positioned at a lower area of the vessel  112 , the purposes of which have been hereinbefore described. 
     Similar to the vessel  12  of  FIGS. 1-3 , the vessel  112  may also include an access door  124  hingedly or pivotally connected to a sidewall of the vessel  112  and moveable between a closed position and an open or access position permitting access to the interior space  118 . The door  124  may likewise include a handle  126  that facilitates movement of the door between the open and closed positions. The door  124 , as discussed above, may be configured so as to define a portion of a boundary of one or more of the windows  120 ,  122 , maximizing the size of the opening in the sidewall of the vessel  112  when the door  124  is in the open position, as disclosed above. 
     With further reference to  FIG. 4 , the interior sidewall of the vessel  112  may include one or more vertical baffles  128  that project into the interior space  118 , as discussed above. 
     As illustrated in  FIG. 4 , the bioreactor system  100 , like bioreactor system  10 , includes a tubing and component management apparatus  140  that facilitates installation and organization of tubing and consumable components of the bioreactor system  100 . The apparatus  140  includes a linear actuator  142  mounted to an exterior sidewall of the vessel  112 . The linear actuator  142  includes a shaft  144  that is selectively extendable and retractable in the vertical direction, denoted by arrow, B, as discussed in detail below. In an embodiment, the linear actuator  142  may take any form capable of moving the shaft  144  vertically such as, for example, a lead screw, a pneumatic actuator or a hydraulic actuator. In an embodiment, the actuator  142  may take the form of a cable and hand crank or motor-driven drive system, similar to that described above in connection with  FIGS. 1-3 . Other linear motion devices known in the art may also be utilized without departing from the broader aspects of the invention. 
     The apparatus  140  further includes a support frame  146  connected to the shaft  144  and moveable vertically therewith under control of the linear actuator  142 . As illustrated in  FIG. 4 , the support frame  146  is suspended over the top opening of the bioreactor vessel  112  via a generally L-shaped support structure  148 . The support frame  146  includes a guide rail  150 , the purposes of which will be hereinafter described. The apparatus  140  also includes a mounting frame  152  that is slidably connected to the support frame  146  and, particularly, the guide rail  150  thereof. The mounting frame  152  is moveable in a horizontal direction away from, and towards, a central axis of the bioreactor vessel, as indicated by arrow, C. In an embodiment, a locking pin  154  is utilized to selectively restrain (or allow) horizontal movement of the mounting frame  152 . 
     In use, when installing a flexible bioprocessing bag prior to bioprocessing, the tubing and component management apparatus  140  starts in an initial position where the linear actuator  142  is extended such that the mounting frame  152  is positioned above the top opening of the bioreactor vessel  112 , as shown in  FIG. 4 . The linear actuator  142  is then utilized to lower the mounting frame  152  into the interior space  118 , as illustrated in  FIG. 5 . The L-shaped support arm  148  of the support frame  146  is configured so that when in a lowered position, the mounting frame  152  is posited about waist-height off of the floor and ergonomically accessible to an operator. As further shown in  FIG. 5 , the door  124  may then be opened, providing easy access to the interior space  118 . With reference to  FIG. 6 , the locking pin  154  can then be withdrawn, enabling the mounting frame  152  to move horizontally along the guide rail  150 , to an extended position where the mounting frame  152  extends through the door opening and out of the interior space  118  of the vessel  112 . The locking pin  154  can be utilized to lock the mounting frame  152  in its extended position, as also shown in  FIG. 6 . 
     In this position, the mounting frame  152  is easily accessible for the mounting of consumable components including, for example, filters  160 , filter heaters and the like to the mounting frame  152 , as well as for the routing and management of tubing, as illustrated in  FIG. 7 . As also shown therein, the flexible bioprocessing bag  20  can easily be installed in the interior space  118  and supported by the mounting frame  152  through one or more hooks  162  coupled to the mounting frame  152  (similar to those described above in connection with  FIGS. 1-3 ). Once all of the tubing has been organized, the bag  20  installed, and various other components mounted securely to the mounting frame  152 , the locking pin  154  is withdrawn and the mounting frame  152  is pushed back into position within the interior space  118  and the locking pin is inserted to retain the mounting frame  152  in such position. The door  124  is then closed, as shown in  FIG. 8 . With reference to  FIG. 9 , the linear actuator  142  is then utilized to move the mounting frame  152  and the attached components to an operational position at a required height above the bioreactor vessel  112  (i.e., generally outside of the interior space  118 ). 
     Similar to the embodiments disclosed above, in an embodiment, the apparatus  140  may be controlled by the control unit (not shown) of the bioreactor system  100  so that the mounting plate  152  can be automatically moved to an installation position (where the mounting plate  152  is extended through the door opening), an operational position (above the bioreactor vessel  112 ), or a deflating position (e.g., moving downwardly continuously or intermittently as the bag  20  is deflated) in dependence upon a selected mode of operation of the bioreactor vessel  100 . 
     In addition to obviating the need for ladders and multiple operators to install the bioprocessing bag and other consumables, the bioreactor system  100  obviates the need of an operator to reach or lean into the interior space within the bioreactor vessel to install such components. In particular, the tubing and component management apparatus  140  is able to move vertically to a position where it can be easily accessed via a door in the sidewall of the vessel, without ladders, and the sliding mounting frame can be extended from the bioreactor vessel in the horizontal direction to provide an even greater ease of installation for such consumable components. The invention therefore provides for easier and quicker installation, as well repeatability in the manner in which the bioprocessing bag is installed. 
     In an embodiment, the tubing and component management apparatus, rather than being mounted to the outside of the vessel as described above, may be integrated with one of the internal baffles (e.g., baffle  28  of vessel  10 ).  FIGS. 10-13  illustrate one such implementation of a tubing and component management apparatus  200 . The apparatus  200  includes a base plate  210  configured for mounting to the interior sidewall of a bioreactor vessel in place of one of the baffles. For example, in an embodiment, as shown in  FIG. 11 , the base plate  210  may include a plurality of flanges  212  having slots configured to receive threaded studs that protruding from the interior sidewall of a bioreactor vessel, for mounting of the base plate to the vessel using nuts that are received on the threaded studs. 
     The apparatus  200  further includes a linear motion rail  214  coupled to the base plate  212 , such as via bolts or other fasteners, a linear motion block  216  slidably coupled to the rail  214  for linear, vertical movement therealong, a carriage plate  218  coupled to the linear motion block  216 , and a mounting frame  220  coupled to the carriage plate  218 . As shown in  FIGS. 10 and 13 , a baffle cover  222  encloses the base plate  210 , the rail  214  and the sliding block  216  and defines a hollow interior space between the cover  222  and the base plate  212 . The baffle cover  222  is shaped so as to provide a substantially equivalent function and performance as typical baffles, and may be, for example, generally triangular in cross section. As best shown in  FIGS. 10 and 13 , the baffle cover  222  includes a slot  224  in an apex thereof, through which the carriage plate  218  extends. The hollow interior and the slot  224  allow for vertical movement of the sliding block  216 , carriage plate  218  and mounting frame  220  along the guide rail  214 , as disclosed hereinafter. 
     As illustrated in  FIGS. 10-12 , the apparatus  200  also includes a cable hoist  226  or other driving mechanism having a cable  228  connected to the carriage plate  218 . The cable hoist  226  may be hand driven or motor driven, and is actuatable to let out the cable  228  or retract the cable  228  to selectively raise or lower the sliding block  216 , and thus the carriage plate  218  and mounting frame  220 , along the rail  214 , to adjust a vertical position of the mounting frame  220 . 
     As shown in  FIGS. 10-13 , the mounting frame  220  may be generally annular or semi-annular in shape, although other shapes and configurations are also envisioned. The mounting frame  220  may include a plurality of filter holder devices  230  for receiving and retaining filters  232 , as well as bag hooks, tubing holders, and similar mounting mechanisms for mounting of an array of consumable components to the mounting frame  220 . 
       FIG. 14  is a top plan view illustrating an exemplary bioreactor vessel  250  with which the tubing and component management apparatus  200  may be utilized. As illustrated therein, the vessel includes a plurality of internal baffles  252 ,  254 ,  256 . In an embodiment, baffle  25 , opposite access door  258 , may be replaced by the tubing and component management apparatus  200 . The tubing and component management apparatus  200 , as disclosed above, may be easily secured to the vessel sidewall, as well as easily removed during transportation as well as in the field, for service. As illustrated in  FIG. 10 , the top of the mounting frame  220  is free from any cables or structures, which makes it easier to install the filters. 
     Similar to the embodiments disclosed above, the apparatus  200  may be controlled by the control unit (not shown) of the bioreactor system so that the mounting frame can be automatically lowered to an installation position before commencement of a bioprocessing operation, and raised to an operational position upon commencement of such operation. 
       FIGS. 15-19  depict another a bioreactor system  500  that is generally similar in configuration to the bioreactor system  100  of  FIGS. 4-9 , where like reference numerals designate like parts. As illustrated therein, the tubing and component management apparatus  140 , however, includes a slightly different mounting frame for the mounting of various consumable components, tubes, and the like. In particular, the apparatus  140 , at the top of the shaft  144  of the linear actuator  140  includes a connector box  510 , and a floating frame  512  connected to the connector box  510 . The floating frame  512  includes a lower frame member  514  that is semi-annular in shape and is configured to support at least one consumable component such as, for example, the flexible bioprocessing bag  20 . The floating frame  512  additional includes an upper frame member  516  that is likewise semi-annular in shape and is configured to support at least one consumable component such as, for example, filters  160  and a pinch valve assembly  518 . The floating frame  512 , and the frame members  514 ,  516  thereof, are mounted so as to be moveable vertically into and out of the interior space  118  within the bioreactor vessel  112  in the manner described above. 
     In particular, in use, when installing a flexible bioprocessing bag  20  prior to bioprocessing, the tubing and component management apparatus  140  starts in an initial position where the linear actuator  142  is extended such that the floating frame  512  and the frame members  514 ,  516  thereof are positioned above the top opening of the bioreactor vessel  112 , as shown in  FIG. 15 . The linear actuator  142  is then utilized to lower the floating frame  512  into the interior space  118 , as illustrated in  FIG. 16 . The door  124  can then be opened (although it envisioned that it can be opened prior to lowering the floating frame  512 ). 
     In this position, the floating frame  512  is easily accessible through the door opening for the mounting of consumable components including, for example, filters  160 , filter heaters, the flexible bag  20 , and a pinch valve assembly  518  for tubing, and the like, to the upper and lower frame members  514 ,  516 , as well as for the routing and management of tubing, as illustrated in  FIG. 17 . As also shown therein, the flexible bioprocessing bag  20  can easily be installed in the interior space  118  and supported by the lower frame member  514 . Once all of the tubing has been organized, the bag  20  installed, and various other components mounted securely to the floating frame  512 , the door  124  is then closed, as shown in  FIG. 18 . With reference to  FIG. 19 , the linear actuator  142  is then utilized to move the floating frame  512  and the attached components to an operational position at a required height above the bioreactor vessel  112  (i.e., generally outside of the interior space  118 ). 
     Similar to the embodiments disclosed above, in an embodiment, the apparatus  140  may be controlled by the control unit (not shown) of the bioreactor system  500  so that the floating frame  512  can be automatically moved to an installation position (where the floating frame is received within the interior space  118  at about waist-height of an operator), an operational position (above the bioreactor vessel  112 ), or a deflating position (e.g., moving downwardly continuously or intermittently as the bag  20  is deflated) in dependence upon a selected mode of operation of the bioreactor vessel/bioprocessing system  500 . 
     The embodiments of the tubing and component management apparatus described herein provide for an ergonomic means of installing the flexible bioprocessing bag, filters, filter heaters, and other consumables, and for organizing the various tubes connected to the bag. In contrast to existing systems, installation of such components can be carried out at waist-height from the side of the bioreactor vessel  12 , obviating the need for multiple operators and stepladders. 
     As indicated above, in addition to present difficulties installing the flexible bioprocessing bag and other components at the top of the bioreactor vessel, properly seating the impeller base plate of the flexible bioprocessing bag on the bottom of the bioreactor vessel during installation of the flexible bag may also present challenges. Accordingly, embodiments of the invention, in addition to providing for tubing and component management for the top of the flexible bag (namely, for tubing and components mounted above the flexible bag at the top of the bioreactor vessel), also provide for management of components at the bottom of the flexible bag. In particular, embodiments of the invention are directed to locating, locking and retaining mechanisms for locking the impeller base plate within the recess (e.g., the impeller base plate recess  30  of  FIG. 3 ), in the bottom of the bioreactor vessel, and an indicating mechanism for indicating that the base plate is properly positioned within the recess. 
     With reference to  FIG. 20 , a component management system  300  in the form of a locking mechanism for an impeller base plate  310  for a bioprocessing system is shown. The system  300  includes a base plate  310  which may be attached to the bottom of a flexible, single-use bioprocessing bag (not shown) for use in stirred-tank bioreactor systems like that shown in  FIGS. 1-19 . The base plate  310  may be mounted within an opening of the bottom of the flexible bioprocessing bag such as by welding, although other means of attachment may also be utilized without departing from the broader aspects of the invention. As is known, the base plate is configured to be received in a corresponding base plate recess  312  in the bottom  314  of a bioreactor vessel. As is known, the base plate  310  serves as an interface between an impeller (not shown) mounted to the base plate  310  interior to the flexible bag, and a rotating magnetic drive head (not shown) outside of the bag beneath the base plate  310 . The base plate  310  may also include a harvest port  316  for the connection of drain tubing to drain the flexible bag, and a plurality of ports  318  for the mounting of a sparger to the base plate  310  interior to the flexible bag. 
     As shown in  FIG. 20 , in an embodiment, the base plate  310  may also include a locating mechanism in the form of a slot  320  that extends from the underside of the base plate  310 . The slot  320  is configured to receive a corresponding tongue  322  that projects into a rearward portion of the recess  312  from the vessel bottom  314 . In an embodiment, it is contemplated that components of the locating mechanism may be reversed, such that the base plate may have a tongue projection that is received in a corresponding slot or groove in the vessel bottom  312 . 
     With further reference to  FIG. 20 , the base plate  310  also includes a latching mechanism on the underside thereof opposite the slot  320 . In an embodiment, the latching mechanism includes a pair of downwardly depending latches  324  that are configured to be received by a corresponding locking/latching mechanism in the vessel bottom  314 , namely, in latch openings  326  in the vessel bottom  314 . In an embodiment, the latches  324  are generally L-shaped and are resilient such that when the base plate  310  is rotated downwardly about the tongue  322 , the base plate  310  is snapped into seated position within the recess  312  and the latches  324  engage the vessel bottom  314  to lock the base plate  310  in position. The latches  324  may be accessed beneath the vessel and pinched inwardly to release the base plate  310  from the recess  312 . While  FIG. 20  illustrates two latches  326 , more than two latches, or a single latch, may be utilized without departing from the broader aspects of the invention. 
     In use, the flexible bag is inserted into the bioreactor vessel and the base plate  310  is angled as illustrated in  FIG. 20  such that the tongue  322  is received in the slot  320  of the base plate  310 . The front of the base plate  310  is then urged downwardly until the latches  326  are received in the recesses  326  and snap into place to retain the base plate  310  in the recess. This ensures the base plate is constrained in all three axes. 
     Turning now to  FIGS. 21-23  another component management system  400  in the form of a locking/latching mechanism for an impeller base plate, according to another embodiment of the invention is shown. The system  400  includes a base plate  410  which may be attached to the bottom of a flexible, single-use bioprocessing bag as described above, and which is configured to be received in a corresponding base plate recess in the bottom of a bioreactor vessel. As is known, the base plate  410  may include a harvest port  416  for the connection of drain tubing to drain the flexible bag, and a plurality of ports  418  for the mounting of a sparger to the base plate  410  interior to the flexible bag. 
     Similar to the base plate  310  of  FIG. 20 , the base plate  410  includes a rear slot  420  that extends from the underside of the base plate  410 , and which is configured to receive a corresponding tongue (not shown) that projects into a rearward portion of the recess in the bottom of the bioreactor vessel. As best shown in  FIGS. 22 and 23 , the base plate  410  also includes a forward catch  430  that extends downwardly from an underside of the base plate  410 . In an embodiment, the catch  430  is generally U-shaped or L-shaped having a catch member  432  that lies in a plane generally parallel to a body of the base plate  410 . 
     As best shown in  FIGS. 21-23 , the system  400  also includes a latch mechanism  440  having a latch member  442  that is configured to engage the catch member  432  of the catch  430 . As illustrated therein, the latch member  442  is attached to the distal end of an elongate shaft  444 , opposite a handle  446 . The shaft  444  extends through a housing  448  and is connected to a spring, e.g., coil spring  450 , within the housing  448 . The spring  450  biases the latch member  442  away from the housing  448  and towards the catch member  432  on the underside of the base plate  410 , as discussed hereinafter. The housing  448  is configured for mounting to the exterior bottom of the bioreactor vessel adjacent to the base plate recess. For example, in an embodiment, the housing  448  may include mounting flanges  452  for attaching the housing  448  to the bottom of the bioreactor vessel using bolts or screws. 
     As best shown in  FIG. 23 , in an embodiment, the latch member  442  may be offset from a longitudinal axis defined by the shaft  444 . As also shown therein, in an embodiment, the latch member  442  may have a sloped or angled contact surface  454 . This surface functions to translate a downward force exerted on the latch member  442  by the catch member  432  into a horizontal force that urges the shaft  44  rearwardly against the spring bias of the coil spring  450 , as discussed hereinafter. 
     In use, the flexible bag is inserted into the bioreactor vessel and the base plate  410  is angled such that the tongue that projects into the recess in the bottom of the bioreactor vessel is received in the slot  420  of the base plate  410 . The front of the base plate  410  is then urged downwardly until the bottom of the catch member  432  contacts the angled surface  454  of the latch member  442  of the latch mechanism  440 . Continued downward urging of the base plate  410  causes the catch member  432  to exert a force on the angled surface  454  of the latch member  442 , causing the latch member  442  and the shaft  444  to move rearwardly against the spring bias of the coil spring  450 . As the catch member  432  passes the lower edge of the angled surface  454 , the spring bias of the coil spring  450  causes the shaft  444  and latch member  442  to translate forwardly, in the direction of arrow, A, in  FIG. 23 . In this position, the latch member  442  extends over the catch member  432 , locking the base plate  410  in position within the recess. This ensures the base plate is constrained in all three axes. In an embodiment, the handle  446  may include a visual feature such as visible demarcation lines or features to indicate a locked and unlocked state of the base plate  410 . 
       FIGS. 24 and 25  are cross sectional views of the base plate  410  in locked position within a recess  460  in the bottom of a bioreactor vessel  462 . As shown, the tongue  464  in the bottom of the vessel  462  is received in the slot  420 , and the latch member  442  engages the catch  430  to retain the base plate  410  in the recess  460 . 
     During unloading of the flexible bag, an operator may simply pull on the handle  446  to move the shaft  444  and latch member  442  against the spring bias, to a position in which the latch member  442  does not engage the catch  430 . In this position, the base plate  410  may be freely rotated out of the recess and removed. 
     Turning now to  FIGS. 26-29  another component management system  500  in the form of a locking/latching mechanism and indicator for an impeller base plate, according to another embodiment of the invention is shown. The system  500  includes a base plate  510  which may be attached to the bottom of a flexible, single-use bioprocessing bag as described above, and which is configured to be received in a corresponding base plate recess in the bottom of a bioreactor vessel. As is known, the base plate  510  may include a harvest port  516  for the connection of drain tubing to drain the flexible bag, and a plurality of ports  518  for the mounting of a sparger to the base plate  510  interior to the flexible bag. 
     Similar to the base plate  310  of  FIG. 20  and base plate  410  of  FIG. 21 , the base plate  510  may include a rear slot (not shown) that extends from the underside of the base plate  510 , and which is configured to receive a corresponding tongue (not shown) that projects into a rearward portion of the recess in the bottom of the bioreactor vessel. As best shown in  FIGS. 27-29 , the base plate  510  also includes a forward catch  530  that extends downwardly from an underside of the base plate  510 . In an embodiment, the catch  530  is generally U-shaped or L-shaped having a catch member  532  that lies in a plane generally parallel to a body of the base plate  510 . 
     As best shown in  FIGS. 27-29 , the system  500  also includes a latch mechanism  540  having a latch member  542  that is configured to engage the catch member  532  of the catch  530 . As illustrated therein, the latch member  542  is attached to the distal end of a shaft  544 , opposite a handle  546 . The shaft  544  extends through a housing  547  and is connected to handle  546 . The housing  547  is configured for mounting to the exterior bottom of the bioreactor vessel adjacent to the base plate recess, as shown in  FIG. 27 . For example, in an embodiment, the housing  547  may include mounting flanges for attaching the housing  547  to the bottom of the bioreactor vessel using bolts or screws. 
     As best shown in  FIG. 27 , in an embodiment, the latch member  542  may be offset from a longitudinal axis defined by the shaft  544 . As also shown therein, in an embodiment, the latch member  542  may have a sloped or angled contact surface  545 . In order to lock the base plate  510  to the bottom of the bioreactor vessel, a user can rotate handle  546 , which cause shaft  554  to extend, resulting in latch member  542  engaging with catch member  532 , as best illustrated in  FIGS. 28-29 . 
     The mechanical locking system  500  for the impeller base plate as described above further includes an indicator mechanism  550 . As best shown in  FIGS. 26, 28, and 29 , the indicator mechanism  550  includes a plunger  552  that extends through the bottom of the bioreactor vessel into the recess. The plunger  552  is attached to a rocker arm  554  that further extends into housing  547 . Located at the end of rocker arm  554  is an indicator  556 . As best shown in  FIGS. 26, 28, and 29 , the indicator is a portion of rocker arm  554  or a pointer (or other visual indicating means) attached to an end of rocker arm  554  (e.g., a cone shaped attachment) along with an indicator panel that is attached to, or a portion of, housing  547 , as illustrated by  FIG. 26 . The indicator panel provides two visual indication regions (i.e., a top portion and a bottom portion), corresponding to a proper positioning and an improper positioning of the base plate  510 . For example, the top portion may be colored green, while the bottom portion may be colored red. 
     In use, prior to attachment of the base plate  510  to the bottom of the bioreactor vessel, plunger  552  is in an extended position such that the top  553  of the plunger  552  protrudes into the recess in the bioreactor vessel. This is accomplished via a biasing mechanism  558  (e.g., a spring) located in a cutout in the bioreactor vessel. In this configuration, and when the base plate  510  is improperly positioned, rocker arm  554  slopes downwardly, such that the indicator  556  is located on the bottom portion of the indicator panel, as best illustrated by  FIG. 28 . When the base plate  510  is properly positioned the plunger  552  is pushed down into the recess, which causes rocker arm  554  to pivot about a pivoting arm  555 , which raises the indicator  556  relative to the indicator panel, as best illustrated by  FIG. 29 . In this way, the indicator  556  moves up and down on the indicator panel, providing an indication of whether the base plate  510  is properly positioned. 
     According to alternative embodiments, the indicator mechanism  500  includes a sensor associated with, or configured to replace, the plunger. The sensor is configured to indicate when the base plate is properly positioned. By way of example, the sensor can be a proximity sensor that emits a signal (e.g., light, electromagnetic radiation) that is configured to indicate when the base plate is properly position. By way of a further example, the sensor can be a mechanical sensor (e.g., a mechanical switch) that is depressed/actuated upon proper placement of the base plate. An output signal from sensor is configured to provide an indication (e.g., visual, tactile, or auditor) when proper placement occurs. 
     According to further alternative embodiments, the rocker arm is configured to move from a position in which it prevents the locking/latching mechanism from engaging the base plate to a position in which the locking/latching mechanism is free to engage the base plate, corresponding to an improper and proper position of the base plate within the recess, respectively. By way of example, the rocker arm may include a portion that is configured to abut a portion of the locking/latching mechanism of any of the aforementioned embodiments, such that when the base plate is improperly positioned the rocker arm prevents the locking mechanism from properly engaging the base plate (e.g., prevents handle  546  fully swinging into position). Only when the base plate is properly position, and thereby the rocker arm moves, is the locking mechanism allowed to engage the base plate. 
     While certain embodiments with regard to the indicator mechanism are discussed above, further indicating mechanisms are within the scope of the present invention, which would be appreciated by one of ordinary skill in the art in light of the present disclosure. 
     It is noted that the location of plunger  552  (or sensor) within the recess of the bottom of the bioreactor vessel, according to embodiments, is generally centrally located. According to a preferred embodiment, and as illustrated by  FIG. 26 , the plunger is located adjacent to a cutout in the bottom of the bioreactor vessel close to the center of recess. This is a preferred location, as it ensures that the plunger is only depressed when the base plate  510  is properly placed. If the plunger is peripherally placed there is a chance that it may be depressed even when the base plate  510  is improperly placed. Advantageously, indicator mechanism  550  provides a clear indication of whether the base plate is properly positioned within the recess. The indicator mechanism further addresses potential problems associated with the difficulties in knowing whether the base plate is properly positioned, even when the front or back of the base plate is latched within the recess. 
     The mechanical locking systems for the impeller base plate described herein provide a means for securely locking the impeller base plate in the recess of the bottom of the bioreactor vessel during installation of the flexible bag. In addition, the mechanisms hereinbefore described provide a tactile, visual or other indication that the base plate is in seated and locked position within the recess. As also described above, while securely locked to the vessel, the base plate can still be easily removed upon completion of a bioprocessing operation. 
     It is contemplated that the base plate locking system described herein in connection with  FIGS. 20-29  may be incorporated into any stirred tank bioreactor vessel known in the art. Still further, it is contemplated that the base plate locking systems of the invention may be incorporated into any the bioreactor vessels illustrated in  FIGS. 2-19 , which include a tubing and component management system, as described herein. In particular, in an embodiment, a bioreactor vessel may include one of, or both of, a base plate locking mechanism for securing the base plate to the bottom of the bioreactor vessel, and a tubing and component management system for arranging and securing consumable components above the flexible bag. The combination of the base plate locking mechanism and the tubing and component management system provides for a bioreactor vessel that facilitates installation of consumable components and provides for an ease of use heretofore not seen in the art. 
     In an embodiment, a bioreactor vessel is provided. The bioreactor vessel includes a bottom, a peripheral sidewall, the bottom and the peripheral sidewall defining an interior space for receiving a flexible bioprocessing bag, a recess in the bottom for receiving a base plate of the flexible bioprocessing bag, a locking mechanism configured to retain the base plate in the recess, and an indicator mechanism configured to indicate when the base plate is properly positioned in the recess. In an embodiment, the locking mechanism includes a latch, wherein the latch is moveable between an engagement position where the latch engages the base plate when the base plate is positioned in the recess in the bottom of the bioreactor vessel to retain the base plate in the recess, and a clearance position where the base plate can be withdrawn from the recess. In an embodiment, the latch is spring-biased toward the engagement position. In an embodiment, the locking mechanism includes a handle that is operable to move the latch from the engagement position to the clearance position. In an embodiment, the latch includes an angled upper surface configured to translate a downward force from the base plate into a lateral force for moving the latch to the clearance position against the spring-bias during installation of the base plate in the recess. In an embodiment, the indicator mechanism includes a plunger, a rocker arm, and an indicator. In an embodiment, the plunger initially protrudes into the recess. In an embodiment, when the base plate is properly placed in the recess, the indictor moves in a direction opposite to movement of the plunger via the rocker arm, which provides the indication of proper placement. In an embodiment, the bioreactor vessel includes a tongue extending into the recess opposite the locking mechanism, the tongue being configured to engage a slot in a rear area of the base plate. In an embodiment, the locking mechanism includes at least one aperture adjacent to the recess and configured to receive at least one corresponding latch of the base plate. In an embodiment, the at least one aperture is a pair of apertures. In another embodiment, a bioprocessing apparatus is provided. The apparatus includes a flexible bioprocessing bag, and a base plate positioned at a bottom of the flexible bioprocessing bag and being shaped so as to be received in a corresponding recess in a bottom of a bioreactor vessel. The base plate includes a locating mechanism adjacent to a rear edge of the base plate, for cooperating with a corresponding locating feature on the bottom of the bioreactor vessel adjacent to the recess to locate the base plate in the recess, and a locking mechanism extending downwardly from an underside of the base plate opposite the locating mechanism, for cooperating with a corresponding locking device of the bioreactor vessel for retaining the base plate in the recess. In an embodiment, the locating mechanism is one of a slot and a tongue, and the corresponding locating feature is the other of a slot and a tongue. In an embodiment, the locking mechanism is a catch lying in a plane generally parallel to, and spaced from, a body of the base plate, and the locking device includes a latch configured to engage the catch. In an embodiment, the locking mechanism is at least one latch member, and the locking device includes a recess configured to receive the at least one latch member. In an embodiment, the at least one latch member is generally L-shaped. In an embodiment, the at least one latch member is resilient. 
     In yet another embodiment, a bioprocessing system is provided. The bioprocessing system includes a bioreactor vessel having a bottom and a peripheral sidewall defining an interior space, a recess in the bottom, a locking mechanism adjacent to the recess, an indicator mechanism, and a flexible bioprocessing bag positionable within the interior space, the flexible bioprocessing bag including a base plate at a bottom of the flexible bioprocessing bag. The base plate is configured to be received in the recess in the bottom of the bioreactor vessel. The locking mechanism is configured to engage the base plate to retain the base plate in the recess. In an embodiment, the locking mechanism includes a latch, and the base plate includes a catch. The latch is moveable between an engagement position where the latch engages the catch when the base plate is positioned in the recess to retain the base plate in the recess, and a clearance position where the base plate can be withdrawn from the recess. In an embodiment, the latch is spring-biased toward the engagement position. In an embodiment, the locking mechanism includes a handle that is operable to move the latch from the engagement position to the clearance position. In an embodiment, the latch includes an angled upper surface configured to translate a downward force from the catch of the base plate into a lateral force for moving the latch to the clearance position against the spring-bias during installation of the base plate in the recess. In an embodiment, the system may also include a tongue extending into the recess opposite the locking mechanism, the tongue being configured to engage a slot in a rear area of the base plate. In an embodiment, the locking mechanism includes at least one aperture adjacent to the recess and configured to receive at least one latch depending downwardly from the base plate opposite the slot. In an embodiment, the indicator mechanism includes a plunger, a rocker arm, and an indicator. In an embodiment, the plunger initially protrudes into the recess. In an embodiment, when the base plate is properly placed in the recess, the indictor moves in a direction opposite to movement of the plunger via the rocker arm, which provides the indication of proper placement. 
     In an embodiment, a bioprocessing system is provided. The bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel and providing access to the interior space, and a tubing and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting of at least one consumable component of the bioprocessing system. The mounting frame is moveable vertically into and out of the interior space. In an embodiment, the mounting frame is moveable between and installation position where the mounting frame is positioned within the interior space of the vessel at a height where the mounting frame is accessible through the access door, and an operational position where the mounting frame is positioned generally above a top of the bioreactor vessel. In an embodiment, the tubing and component management apparatus includes a lifting mechanism for moving the mounting frame vertically along a centerline of the vessel. In an embodiment, the mounting frame is slidable in a direction generally perpendicular to the centerline of the vessel between a stowed position where the mounting frame is positioned within the interior space, and an access position where the mounting frame extends through an access door opening when the access door is in an open position. In an embodiment, the tubing and component management apparatus includes a locking device for selectively locking the mounting frame in the stowed position and the access position. In an embodiment, the lifting mechanism is a linear actuator. In an embodiment, the tubing and component management apparatus includes a support member mounted to a sidewall of the vessel, a boom extending from the support member generally over the vessel, and a sleeve extending downwardly from the boom along a centerline of the vessel, wherein the mounting frame includes a shaft that is received within the sleeve. In an embodiment, the lifting mechanism includes a cable extending from the mounting frame, through the sleeve and along the boom, wherein the cable is selectively extendable and retractable to selectively lower and raise the mounting frame. In an embodiment, the lifting mechanism is integrated with an internal baffle of the vessel. In an embodiment, the tubing and component management apparatus includes a guide rail mounted to an internal sidewall of the vessel and a carriage plate slidably connected to the guide rail, wherein the mounting frame is connected to the carriage plate for vertical movement along the guide rail. In an embodiment, the tubing and component management apparatus includes a baffle cover defining the internal baffle, wherein the baffle cover includes a slot through which the carriage plate extends. In an embodiment, the vessel includes a window in a sidewall of the vessel. The access door is movable between a closed position and an open position. When in the closed position, an edge of the access door defines at least a portion of a boundary of the window. In an embodiment, the mounting frame includes at least one slot, aperture or bracket for receiving the at least one consumable component. In an embodiment, the at least one consumable component is a tube, a filter or a filter heater. 
     In another embodiment, a method for installing components of a bioprocessing system is provided. The method includes the steps of lowering a mounting frame into a vessel through a top opening of the vessel, opening an access door in a sidewall of the vessel to access the mounting frame, mounting at least one consumable component to the mounting frame, closing the access door, and raising the mounting frame to a position adjacent to a top of the vessel. In an embodiment, the method also includes the step of moving the mounting frame in a direction generally perpendicular to a centerline of the vessel to extend the mounting frame through the access door opening. In an embodiment, the steps of lowering the mounting frame and raising the mounting frame are carried out automatically by a control unit of the bioprocessing system. In an embodiment, the method also includes actuating a lift assembly to lower or raise the mounting frame. 
     In yet another embodiment, a bioprocessing system is provided. The bioprocessing system includes a vessel defining an interior space for receiving a flexible bioprocessing bag, the vessel having an access door in a sidewall of the vessel and providing access to the interior space through an access door opening, and a tubing and component management apparatus mounted to the sidewall of the vessel and having a mounting frame for mounting of at least one consumable component of the bioprocessing system. The mounting frame is moveable between and installation position where the mounting frame is positioned within the interior space of the vessel at a height where the mounting frame is accessible through the access door, and an operational position where the mounting frame is positioned generally above a top of the bioreactor vessel. The tubing and component management apparatus includes a lift mechanism for moving the mounting frame between the installation position and the operational position. In an embodiment, the mounting frame is slidable in a direction generally perpendicular to the centerline of the vessel between a stowed position where the mounting frame is positioned within the interior space, and an access position where the mounting frame extends through an access door opening when the access door is in an open position. In an embodiment, the tubing and component management apparatus is mounted to an internal side of the sidewall of the vessel. In an embodiment, the lift mechanism includes a linear actuator. In an embodiment, the vessel includes a window in a sidewall of the vessel, wherein the access door is movable between a closed position and an open position, and wherein when in the closed position, an edge of the access door defines at least a portion of a boundary of the window. 
     As used herein, an element or step recited in the singular and proceeded with the word “a” or “an” should be understood as not excluding plural of said elements or steps, unless such exclusion is explicitly stated. Furthermore, references to “one embodiment” of the present invention are not intended to be interpreted as excluding the existence of additional embodiments that also incorporate the recited features. Moreover, unless explicitly stated to the contrary, embodiments “comprising,” “including,” or “having” an element or a plurality of elements having a particular property may include additional such elements not having that property. 
     This written description uses examples to disclose several embodiments of the invention, including the best mode, and also to enable one of ordinary skill in the art to practice the embodiments of invention, including making and using any devices or systems and performing any incorporated methods. The patentable scope of the invention is defined by the claims, and may include other examples that occur to one of ordinary skill in the art. Such other examples are intended to be within the scope of the claims if they have structural elements that do not differ from the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal languages of the claims.