Patent Publication Number: US-2004058326-A1

Title: Identification and use of molecules implicated in pain

Description:
[0001] This application claims the priority of United Kingdom application NO. 0118354.0, filed Jul. 27, 2001, and United Kingdom application NO. 0202883.5, filed Feb. 7, 2002; the entire contents of which applications are hereby incorporated by reference herein.  
       FIELD OF THE INVENTION  
       [0002] The present invention relates to nucleic acids, their expression products and pathways involved in pain, and their use in screening for molecules that can alleviate pain. The invention further relates to methods for the assay and diagnosis of pain in patients.  
       BACKGROUND TO THE INVENTION  
       [0003] Pain is currently classified into four general types. Post-operative acute pain can be successfully treated with existing pain medications of e.g. the opioid and non-steroidal anti-inflammatory (NSAID) types, and is usually short-term and self-limiting. A second type of pain, present e.g. in cancer and arthritis, is also responsive to medication initially with a NSAID and in its later stages with opioids. Neuropathic pain arises from damage to the central or peripheral nerve systems, and is more effectively treated with antidepressants or anticonvulsants. A fourth type of pain called central sensitization results from changes in the central nervous system as a result of chronic pain, these changes often being irreversible and difficult to treat. Nerve pain from shingles or diabetes falls into this and the neuropathic category. Changes occur where pain is at first poorly controlled and gradually progress to the point where a person is sensitive to stimuli which would not normally cause pain, for example a light touch. People with pain of this kind often describe a widening of the pain area to include areas which had originally not been injured or which were thought not to be involved in pain. This classification is, however based on clinical symptoms rather than on the underlying pain mechanisms.  
       [0004] Opiates such as morphine belong to a traditional class of pain-relieving compounds that are now recognized as binding to opiate receptors. Naturally occurring polypeptides have also been found to have opiate-like effects on the central nervous system, and these include β-endorphin, met-enkephalin and leu-enkephalin.  
       [0005] Salicin was isolated at the beginning of the 19th century, and from that discovery a number of NSAIDs such as aspirin, paracetamol, ibuprofen, flurbiprofen and naproxen were developed. NSAIDs are by far the most widely used pain-relieving compounds, but can exhibit side effects, in particular irritation of the GI tract that can lead to the formation of ulcers, gastrointestinal bleeding and anemia.  
       [0006] Interest in the neurobiology of pain is developing: see a colloquium sponsored by the US National Academy of Sciences in December 1998 concerning the neurobiology of pain and reviewed in  The Scientist  13[1], 12, 1999. Many pain mechanisms were discussed including the role of the capsaicin receptors in pain, (M. J. Caterina et al.,  Nature , 389, 816-824, 1997). Large dosages of capsaicin were reported to disable that receptor, (W. R. Robbins et al.,  Anesthesia and Analgesia , 86, 579-583, 1998). Additionally, a tetrodotoxin-resistant sodium channel found in small diameter pain-sensing neurons (PN3) was discussed (A. N. Akopian et al.,  Nature , 379, 257-262, 1986) and L. Sangameswaran et al.,  Journal of Biological Chemistry , 271, 953-956, 1996). Its involvement in transmission and sensitization to pain signals has been reported, (S. D. Novakovic et al.,  Journal of Neuroscience , 18, 2174-2187, 1998). A further tetrodotoxin-resistant sodium channel has been reported (S. Tate et al.,  Nature Neuroscience , 1, 653-655 1998).  
       [0007] Second messenger systems have also been shown to be important since knockout-mice lacking protein kinase C (PKC) γ were reported to respond to acute pain e.g. from a hot surface, but not to respond to neuropathic pain when their spinal nerves are injured (Malmberg et al.,  Science , 278, 279-283 (1997).  
       [0008] Present methods for identifying novel compounds that relieve pain of one or more of the types indicated above suffer from the defect that they are dependent either on the relatively limited number of receptors known to be involved in pain or on the empirical identification of new receptors which is an uncertain process. In relation to known receptors, for example the opioid receptor, research directed to improved compounds offers the possibility of screenining compounds that have a better therapeutic ratio and fewer side effects. This does not lead naturally to compounds for different pain receptors that have new modes of action and new and qualitatively different benefits. Even when newly identified additional receptors are taken into account, known receptors revolve around tens of gene products. However, there are between 30,000 and 40,000 genes in the genome of an animal and more of them are concerned with nervous system function than with peripheral function. We therefore concluded that a large number of receptors and pathways are important to the transduction of pain, but up to now have remained unknown.  
       SUMMARY OF THE INVENTION  
       [0009] It is an object of the invention to provide sequences of genetic material for which no role in pain has previously been disclosed, and which are useful, for example, in:  
       [0010] identifying metabolic pathways for the transduction of pain  
       [0011] identifying from said metabolic pathways compounds having utility in the diagnosis or treatment of pain  
       [0012] producing proteins and polypeptides with a role in the transduction of pain;  
       [0013] producing genetically modified non-human animals that are useful in the screening of compounds having utility in the treatment or diagnosis of pain.  
       [0014] Identifying ligand molecules for receptors involved in said metabolic pathways and having utility in the treatment of pain.  
       [0015] It is yet a further object of the invention to provide research tools, for example non-human animals and microorganisms, that can be used in screening compounds for pharmacological activity, especially pain-reducing activity.  
       [0016] The present invention is based on sequences that are down-regulated in two models of chronic pain, namely streptozocin-induced diabetes and chronic constrictive injury (CCI) to a nerve leading to the spine, for example the sciatic nerve.  
       [0017] In one aspect, the invention relates to the use in the screening of compounds that are effective in the treatment of pain, or in the diagnosis of pain, of:  
       [0018] (a) an isolated gene sequence that is down regulated in the spinal cord of a mammal in response to first and second models of pain, for example in response to streptozocin-induced diabetes and in response to a chronic constrictive injury to a nerve leading into the spine;  
       [0019] (b) an isolated gene sequence having at least 80% sequence identity with any of the nucleic acid sequences of Tables I-VI, preferably 85% sequence identity, more preferably 90%, increasingly preferably 95%, most preferably 99%.  
       [0020] (c) an isolated nucleic acid sequence comprising a sequence that is hybridizable to any of the gene sequences according to (a) or (b) under stringent hybridisation conditions;  
       [0021] (d) a recombinant vector comprising any of the gene sequences according to (a) to (c);  
       [0022] (e) a host cell containing the vector according to (d);  
       [0023] (f) a non-human animal, for use in the screening of compounds that are effective in the treatment of pain, or in the diagnosis of pain, having in its genome an introduced gene sequence or a removed or down-regulated nucleotide sequence, said sequence becoming down-regulated in the spinal cord of a mammal in response to first and second models of pain, particularly neuropathic or sensitisation pain, for example in response to streptozocin-induced diabetes and in response to a chronic constrictive injury to a nerve leading into the spine;  
       [0024] (g) an isolated polypeptide containing an amino acid sequence at least 90% identical to an amino acid sequence encoded by a nucleotide sequence according to any one of (a) to (d), or a variant thereof with sequential amino acid deletions from the C terminus and/or the N-terminus; or  
       [0025] (h) an isolated antibody that binds specifically to the isolated polypeptide according to (g).  
       [0026] The invention further provides a compound that is useful in the treatment or diagnosis of pain and that modulates the action of an expression product of a gene sequence that becomes down-regulated in the spinal cord of a mammal in response to first and second models of pain, for example being down-regulated both in response to streptozocin induced diabetes and in response to chronic constrictive injury to a nerve leading into the spine.  
       [0027] The invention also relates to the use of naturally occurring compounds such as peptide ligands of the expression products of the above gene sequences and their associated signal transduction pathways for the treatment of pain.  
       DESCRIPTION OF PREFERRED EMBODIMENTS  
       [0028] Definitions  
       [0029] Within the context of the present invention:  
       [0030] “Comprising” means consisting of or including. Thus nucleic acid comprising a defined sequence includes nucleic acid that may contain a full-length gene or full-length cDNA. The gene may include any of the naturally occurring regulatory sequence(s), such as a transcription and translation start site, a promoter, a TATA box in the case of eukaryotes, and transcriptional and translational stop sites. Further, nucleic acid comprising a cDNA or gene may include any appropriate regulatory sequences for the efficient expression thereof in vitro.  
       [0031] “Isolated” requires that the material be removed from its original environment (e.g. the natural environment if it is naturally occurring). For example, a naturally occurring polynucleotide or a peptide present in a living animal is not isolated, but the same polynucleotide or peptide, separated from some or all of the coexisting materials in the natural system, is isolated. Such polynucleotide can be part of a vector and/or such polynucleotide or peptide can be part of a composition, and still be isolated in that the vector or composition is not a part of its natural environment.  
       [0032] “Mechanistically distinct” in relation to pain models implies that the pain is induced by mechanisms that differ in kind rather than being variants of a similar pain model. Thus diabetic pain and chronic constrictive pain models are mechanistically distinct, whereas spinal nerve ligation models and sciatic nerve ligation models which both work by ligation are not.  
       [0033] “Purified” does not require absolute purity; instead it is intended as a relative definition. Purification of starting materials or natural materials from their native environment to at least one order of magnitude, preferably two or three orders, and more preferably four or five orders of magnitude is expressly contemplated.  
       [0034] “Nucleic acid sequence” or “gene sequence” means a sequence of nucleotides or any variant or homologue thereof, or truncated or extended sequence thereof, and is preferably indicated by a Genebank accession number. Also within the scope of the present invention are down-regulated nucleic acid sequences which encode expression products which are components of signaling pathways. This invention also includes any variant or homologue or truncated or extended sequence of the down-regulated nucleotide sequence. Also within the scope of the present invention, the term “nucleic acid(s) product”, or “expression product” or “gene product” or a combination of these terms refers without being biased, to any, protein(s), polypeptide(s), peptide(s) or fragment(s) encoded by the down-regulated nucleotide sequence.  
       [0035] “Operably linked” refers to a linkage of polynucleotide elements in a functional relationship. For instance, a promoter or an enhancer is operably linked to a coding sequence if it regulates the transcription of the coding sequence. In particular, two DNA molecules (such as a polynucleotide containing a promoter region, and a polynucleotide encoding a desired polypeptide) are said to be “operably linked” if the nature of the linkage between the two polynucleotides does not (1) result in the introduction of a frame-shift mutation and (2) interfere with the ability of the polynucleotide containing the promoter to direct the transcription of the coding polynucleotide.  
       [0036] “Gene product” refers to polypeptide—which is interchangeable with the term protein—which is encoded by a nucleotide sequence and includes single-chain polypeptide molecules as well as multiple-polypeptide complexes where individual constituent polypeptides are linked by covalent or non-covalent means.  
       [0037] Polypeptides of the present invention may be produced by synthetic means (e.g. as described by Geysen et al., 1996) or by recombinant means.  
       [0038] The terms “variant”, “homologue”, “fragment”, “analogue” or “derivative” in relation to the amino acid sequence for the polypeptide of the present invention includes any substitution of, variation of, modification of, replacement of, deletion of or addition of one (or more) amino acid from or to the sequence providing the resultant polypeptide has the native gene product activity. In particular, the term “homologue” covers homology with respect to structure and/or function. With respect to sequence homology, there is at least 90%, more preferably at least 95% homology to an amino acid sequence encoded by the relevant nucleotide sequence shown in Tables I-VI, preferably there is at least 98% homology.  
       [0039] Typically, for the variant, homologue or fragment of the present invention, the types of amino acid substitutions that could be made should maintain the hydrophobicity/hydrophilicity of the amino acid sequence. Amino acid substitutions may include the use of non-naturally occurring amino acid analogues.  
       [0040] In addition, or in the alternative, the protein itself could be produced using chemical methods to synthesize a polypeptide, in whole or in part. For example, peptides can be synthesized by solid phase techniques, cleaved from the resin, and purified by preparative high performance liquid chromatography (e.g. Creighton (1983) Proteins Structures and Molecular Principles, W H Freeman and Co., New York, N.Y., USA). The composition of the synthetic peptides may be confirmed by amino acid analysis or sequencing (e.g. the Edman degradation procedure).  
       [0041] Direct peptide synthesis can be performed using various solid-phase techniques (Roberge J Y et al Science Vol 269 1995 202-204) and automated synthesis may be achieved, for example, using the ABI 431 A Peptide Synthesizer (Perkin Elmer) in accordance with the instructions provided by the manufacturer. Additionally, the amino acid sequence of a gene product, or any part thereof, may be altered during direct synthesis and/or combined using chemical methods with a sequence from other subunits, or any part thereof, to produce a variant polypeptide.  
       [0042] In another embodiment of the invention, a gene product natural, modified or recombinant amino acid sequence may be ligated to a heterologous sequence to encode a fusion protein. For example, for screening of libraries for compounds and peptide agonists and antagonists of gene product activity, it may be useful to encode a chimeric gene product expressing a heterologous epitope that is recognised by a commercially available antibody. A fusion protein may also be engineered to contain a cleavage site located between a gene product sequence and the heterologous protein sequence, so that the gene product may be cleaved and purified away from the heterologous moiety.  
       [0043] The gene product may also be expressed as a recombinant protein with one or more additional polypeptide domains added to facilitate protein purification. Such purification facilitating domains include, but are not limited to, metal chelating peptides such as histidine-tryptophan modules that allow purification on immobilised metals (Porath J, Protein Expr Purif Vol 3 1992 p263-281), protein A domains that allow purification on immobilised immunoglobulin, and the domain utilised in the FLAGS extension/affinity purification system (Immunex Corp, Seattle, Wash., USA). The inclusion of a cleavable linker sequence such as Factor XA or enterokinase (Invitrogen, San Diego, Calif., USA) between the purification domain and the gene product is useful to facilitate purification.  
       [0044] “Pain” includes chronic pain, neuropathic pain, pain arising from central sensitisation, and in particular diabetic pain.  
       [0045] “Stringent hybridization conditions” is a recognized term in the art and for a given nucleic acid sequence refers to those conditions which permit hybridization of that sequence to its complementary sequence and closely homologous sequences. Conditions of high stringency may be illustrated in relation to filter-bound DNA as for example 2×SCC, 65° C. (where SSC=0.15M sodium chloride, 0.015M sodium citrate, pH 7.2), or as 0.5M NaHPO 4 , 7% sodium dodecyl sulfate (SDS), 1 mM EDTA, at 65° C., and washing in 0.1×SCC/0.1% SDS at 68° C. (Ausubel F. M. et al., eds, 1989 , Current Protocols in Molecular Biology , Vol. I, Green Publishing Associates, Inc., and John Wiley &amp; Sons Inc., New York, at p. 2. 10.3). Hybridization conditions can be rendered highly stringent by raising the temperature and/or by the addition of increasing amounts of formamide, to destabilize the hybrid duplex of non-homologous nucleic acid sequence relative to homologous and closely homologous nucleic acid sequences. Thus, particular hybridisation conditions can be readily manipulated, and will generally be chosen depending on the desired results.  
       [0046] “Variants or homologues” include (a) sequence variations of naturally existing gene(s) resulting from polymorphism(s), mutation(s), or other alteration(s) as compared to the above identified sequences, and which do not deprive the encoded protein of function (b) recombinant DNA molecules, such as cDNA molecules encoding genes indicated by the relevant Genebank accession numbers and (c) any sequence that hybridizes with the above nucleic acids under stringent conditions and encodes a functional protein or fragment thereof.  
       [0047] Identified Sequences  
       [0048] The inventors have identified nucleotide sequences that give rise to expression products listed in Tables I-VI, that become differentially expressed in the spinal cord in response to two distinct chronic pain stimuli, for example neuropathic and/or central sensitization pain stimuli, and that are believed to be involved in the transduction of pain. In Tables I-VI, * denotes more preferred nucleic acid sequences and ** denotes most preferred nucleic acid sequences. These nucleic acid sequences have not previously been implicated in the transduction of pain.  
       [0049] The validity of the present experimental procedure was confirmed by the fact that nucleotide sequences were obtained as a result of the investigation whose function in the transduction of pain has been previously confirmed and established. These nucleic acid sequences are not part of this invention. Any of the nucleic acid sequences and expression products can be used to develop screening technologies for the identification of novel molecules for the prevention or treatment of pain. These screening technologies could also be used to ascribe new pain therapeutic indications to molecules that have not previously been identified as being useful for the prevention or treatment of pain. Furthermore, the said nucleic acid sequences can be used as diagnostic tools and for the development of diagnostic tools.  
               TABLE I                          Sequences whose expression products are kinases                                     Expression                           product   Rat   Mouse   Human       or name    Accession   accession   Accession       (Reference)   Number   number   Number   Reaction   Assay               Pyruvate   M24359   X97047   X56494   Tissue-   Kinase       kinase, M1   (SEQ ID           specific       and M2   No&#39;s 1-3)           promoter;       subunit s               Carbo-       (Refs: 1-2)               hydrate                       kinase                  
 
       [0050]               TABLE II                          Sequences whose expression products are receptors                                     Expression                           Product or   Rat   Mouse   Human       Name   Accession   accession   Accession       (Reference)   Number   Number   Number   Reaction   Assay               Dopamine   I58000           Dopamine   Receptor       receptor   (SEQ ID           receptor       D.sub.1 **   NO 4)       (Ref: 3)       Putative       AF114168       GABA-B   Receptor       GABA-B1a       (SEQ ID       Receptor       receptor **       No&#39;s 5-6)       (Ref: 4)                    
       [0051]               TABLE III                          Sequences whose expression products are transporters                                     Expression   Rat   Mouse   Human               Product or   Accession   Accession   Accession       Name   Number   Number   Number   Reaction   Assay               Differentiation-   AF271235           Trans-   Transporter       associated Na-   (SEQ ID           membrane       dependent   NO&#39;s 7-8)           phosphate       inorganic               transport       phosphate       cotransporter       (Ref: N/A)       Putative vacuolar   U87309       AAM47563.1   Vacuolar   Transporter       assembly protein           (SEQ ID No   assembly       VSP41 gene           9)   and traffic       (Ref: N/A)                    
       [0052]               TABLE IV                          Sequences whose expression products are G-protein coupled receptor       proteins                                     Expression   Rat   Mouse   Human               product or   Accession   Accession   Accession       Name   Number   Number   Number   Function   Assay               Git1 (G-protein-   AF085693           Regulation of   Ligand binding       coupled receptor               activity of       kinase-interactor   (SEQ ID NO           ARF6 in       1; GPCR kinase-   10-11)           phosphatidyl-       associated ADP-               inisitol 3-kinase       ribosylation               signalling       factor)               pathways; β2       (Ref: 5)               adrenergic                       receptor                       regulation                    
       [0053]               TABLE V                          Sequences whose expression products are DNA-binding proteins                                     Expression   Rat   Mouse   Human               Product or   Accession   Accession   Accession   Re-       Name   Number   Number   Number   action   Assay               Putative       X91866   M11354   DNA   DNA       histone H3.3A           (SEQ ID NO   binding   binding       (Ref: 6)           12-13)                    
       [0054]               TABLE VI                          Sequences whose expression products are other enzymes                                     Expression   Rat   Mouse   Human               product or   Accession   Accession   Accession       Name   Number   Number   Number   Reaction   Assay               3-Hydroxy 3-   X52625           Cholesterol   Ligase       methylglutaryl               biosynthesis       coenzyme A   (SEQ ID       synthase,   No&#39;s 14-15)       cytosolic *       (Ref: 7)       Acyl-CoA   D360666           Fatty acid   Ligase       synthetase II,   (N/A)           metabolism       brain (Ref: N/A)       Farnesyl   M34477           Isoprene   Ligase       diphosphate               biosynthesis       synthase **   (Seq ID No&#39;s       (Ref: 8)   16-17)       Bendless protein   AB032739       E12457   Protein   Ligase       (Ref: N/A)   (Seq ID No&#39;s           degradation           18-19)       fatty acid   X62888           Fatty acid   Ligase       synthase   (SEQ ID           synthesis       (Ref: 9)   No&#39;s 20-21)       Glutamine   M91652           Amino acid   Ligase       synthetase (EC   (SEQ ID           metabolism       6.3.1.2) **   NO&#39;s       (Ref: 10)   22-23)       Putative seryl-           X91257   Ligase   Ligase       tRNA synthetase           (SEQ ID NO&#39;s       (Ref No: 11)           24-25)       Enolase, alpha   X02610   X52379   M14328   Glycolysis   Lyase       alpha, non-           (SEQ ID NO&#39;s       neuronal (NNE)           26-27)       (REF NO 12)       Aldose reductase,   X05884           Reduces   Oxidoreductase       lens (AREC   (SEQ ID           carbonyl       11.1.21)   NO&#39;s 28-29)       (REF: 13)       Cytochrome-c   S79304           Mitochrondrial   Oxidoreductase       oxidase I,   (SEQ ID           energy       mitochondrial   NO&#39;s 30-31)           metabloism       (Ref: 14)       Lactate   U07181   X51905   Y00711   Glycolysis   Oxidoreductase       dehydrogenase-B   (SEQ ID       (LDH-B)   NO&#39;s 32-33)       (Ref: 15)       Putative           x13923   Mitochrondrial   Oxidoreductase       cytochrome c               energy       oxidase VIB (EC           (SEQ ID NO&#39;s   metabolism       1.9.3.1)           34-35)       (Ref: 16)       Putative NADH:           AF044953   Mitochrondrial   Oxidoreductase       ubiquinone           (SEQ ID No&#39;s   energy       oxidoreductasePG           36-37)   metabolism       IV subunit       (Ref: 17)       Putative succinate       AF095938   AF171022   TCA cycle   Oxidoreductase       dehydrogenase       (SEQ ID       flavoprotein       No&#39;s 38-       (Ref: N/A)       39)       Putative           J04973   Mitochrondrial   Oxidoreductase       ubiquinol-           (SEQ ID No&#39;s   energy       cytochrome-c           40-41)   metabolism       reductase (EC       1.10.2.2) core       protein II       (REF: 18)       Stearoyl-coA   AB032243   M26270       Fatty acid   Oxidoreductase       desaturase 2 *   (SEQ ID           biosynthesis       (Ref: N/A)   NO&#39;s 42-43)       Ribophorin I *   X05300           Glycosolation   Transferase       (Ref: 19)   (SEQ ID           No&#39;s 44-45)       Sulfotransferase-   AF188699           Transferase   Transferase       like protein   (SEQ ID       (REF: 20)   No&#39;s 46-47)       ATP synthase,   X56133           Mitochrondrial   Hydrolase       H+, alpha   (SEQ ID           energy       subunit,   No&#39;s 48-49)           metabolism       mitochondrial       (EC 3.6.1.34)       (Ref: N/A)       F1F0 ATPase   U00926           Mitochrondrial   Hydrolase       delta subunit   (SEQ ID           energy       (REF: 21)   No&#39;s 50-51)           metabolism       Putative           D78013   Pyrimidine   Hydrolase       dihydropyrimidin           (SEQ ID NO&#39;s   degradation       ase related protein           52-53)       * (REF: 22)       Heat shock   545392   M18186   M16660   Cell protection   Hydrolase       protein 90   (SEQ ID       (REF: 23)   NO&#39;s 114-           115)       Myelin basic   K00512           Myelin   Ligand binding       protein S (MBP   SEQ ID No           structural       S) (REF: 24)   54)           protein       Transferrin   D38380           Iron transport   Ligand binding       (Ref 25)   (SEQ ID           No&#39;s 55-56)       Neurofilament,   AF031880           Cytoskeleton   Ligand binding       light molecular   (SEQ ID       weight (NF-L)   No&#39;s 57-58)       (Ref: 26)       Myelin-   M16800   M31811       Cell adhesion   Ligand binding       associayted   (SEQ ID           molecule for       glycoprotein   NO&#39;s 59-60)           postnatal neural       (MAG)               development       (Ref: 27)       NF-M middle   M18628           Cytoskeleton   Ligand binding       molecular weight   (SEQ ID       neurofilament   No&#39;s 61-62)       protein       (Ref: 28)       Neuro-   D32249           Protein sorting;   Ligand binding       degeneration   (SEQ ID           synaptic       associated-   No&#39;s 63-64)           communication       protein 1               and plasticity       (Ref: 29)       S-100 protein β-   X01090           Zinc and   Ligand binding       subunit   (SEQ ID No           calcium binding       (REF: 30)   65)       Microtubule-   X60370       L06237   Cytoskeleton   Ligand binding       associated protein   (SEQ ID           protein,       1b (Map 1b)   No&#39;s 66-67)           neuronal       (Ref: 31)               growth/                       regeneration;                       microtubule                       binding protein       Putative cdc 37   D26564           Cell signalling;   Ligand binding       homolog   (Seq ID No&#39;s           cell cycle       (Ref: 32)   68-69)           protein       Putative ras-           U11293   Small GTP   Ligand binding       related protein           (SEQ ID No&#39;s   binding protein       Rab-5c           70-71)       (Ref: 33)       Putative gelsolin       J04953       Cytoskeleton   Ligand binding       (Ref: 34)       (SEQ ID       protein               No&#39;s 72-               73)       Cd81 antigen   U19894   X59047   M33680   Regulator for   Ligand binding       (target of   (SEQ ID           neuron-induced       antiproliferative   No&#39;s 74-75)           astrocyte       antibody 1)               differentiation       (Ref: 35)               microglial                       effector                       functions       Mobp81 (Myelin-   X87900           Myelin   Ligand binding       associated/Oligo-   (SEQ ID           compaction       dendrocytic basic   N0&#39;s 76-77)       protein 81)       (Ref: 36)       Syntaxin binding           BC002869   Neuro-   Ligand binding       protein n-sec1,           (Seq ID No&#39;s   transmission       sec1 homolog           78-79)       (Ref: N/A)       Alpha-internexin   M73049           Cytoskeleton   Ligand binding       (Ref: 37)   (SEQ ID           protein;           No&#39;s 80-81)           neuronal                       intermediate                       filament that                       can self-                       assemble       Putative β-       AB024921       Cytoskeleton   Ligand binding       sarcoglycan A3b       (SEQ ID       protein       (Ref: N/a)       No&#39;s 82-               83)       CGI-78 protein   AF151835       AF151835   Unknown   Ligand binding       Ref: 38)           (SEQ ID No&#39;s                   84-85)       KIAA0143           D63477   Transmembrane   Ligand binding       (Ref: 39)           (SEQ ID NO&#39;s   protein                   86-87)       Septin 2           D50918   GTPase;   Ligand binding       (KIAA0128;           (SEQ ID No&#39;s   cytokinesis       Ref 39)           88-89)       Nucleobindin   Z36277           Unknown   Ligand binding       (Ref: 40)   (SEQ ID           No&#39;s 90-91)       Myelin protein   M69139   S55427       Myelin   Ligand binding       SR13   (SEQ ID           structural       (Ref: 41)   NO&#39;s 92-93)           protein       B-Actin,   V01217   X03672       Structural   Ligand binding       cytoplasmic   (SEQ ID           protein       (Ref: 42)   NO&#39;s 94-95)       Ly6/neurotoxin       AF141377       Neuro-   Ligand binding       (Lynx1) homolog       (SEQ ID       transmitter       (Ref: 43)       No&#39;s 96-               97)       modulator       Astrocytic   AJ243949   X86694       PKC substrate   Ligand binding       phosphoprotein,   (SEQ ID       PFA 15 gene   NO&#39;s 98-       (Ref: N/A)   99)       PLIC-1       AF177345       Cytoskeleton   Ligand binding       (Ref: N/A)       (SEQ ID       interaction               NO 100-               101)       Nfx1 (tip   AF093139   AF093140       Unknown   Logand binding       associating   (SEQ ID       protein (TAP)   NO&#39;s 102-       gene) (Ref: N/A)   103)       Alpha-Crystallin   U04320   M73741   M28638   Stress   Ligand binding       B (Ref N/A)   SEQ ID           response; heat           NO&#39;s 104-           shock element           105)       Heat shock-like   X70065   U73744   Y00371   Stress response   Ligand binding       protein 70 kD   (SEQ ID       (Ref: 44)   No&#39;s 106-           107)       Tau microtubule-   X79321           Microtubule   Ligand binding       associated protein   (SEQ ID           associated       (Ref: 45)   NO&#39;s 108-           protein           109)           expressed in                       neurons       Myelin,   K03242           Myelin   Ligand binding       Schwann cell,   (SEQ ID           structural       Peripheral (P-0)   No&#39;s 110-           protein       (Ref: 46)   111)       B-Tubulin class 1   AB011679   X04663   AF14139   Structural   Ligand binding       (Ref: 47)   (SEQ ID           protein           NO&#39;s 112-           113)       Putative           AF116910   RNA hydrolysis   Hydrolase       ribonuclease III           (SEQ ID NO&#39;s       (Ref: N/A)           116-117)                    
       [0055] Production of Polypeptides Nucleic Acids  
       [0056] Vectors  
       [0057] Recombinant expression vectors comprising a nucleic acid can be employed to express any of the nucleic acid sequences of the invention. The expression products derived from such vector constructs can be used to develop screening technologies for the identification of molecules that can be used to prevent or treat pain, and in the development of diagnostic tool for the identification and characterization of pain. The expression vectors may also be used for constructing transgenic non-human animals.  
       [0058] Gene expression requires that appropriate signals be provided in the vectors, said signals including various regulatory elements such as enhancers/promoters from viral and/or mammalian sources that drive expression of the genes or nucleic acid sequences of interest in host cells. The regulatory sequences of the expression vectors used in the invention are operably linked to the nucleic acid sequence encoding the pain-associated protein of interest or a peptide fragment thereof.  
       [0059] Generally, recombinant expression vectors include origins of replication, selectable markers, and a promoter derived from a highly expressed gene to direct transcription of a downstream nucleoide sequence. The heterologous nucleotide sequence is assembled in an appropriate frame with the translation, initiation and termination sequences, and if applicable a leader sequence to direct the expression product into the periplasmic space, the extra-cellular medium or cell membrane.  
       [0060] In a specific embodiment wherein the vector is adapted for expressing desired sequences in mammalian host cells, preferred vectors will comprise an origin of replication from the desired host, a suitable promoter and an enhancer, and also any necessary ribosome binding sites, polyadenylation site, transcriptional termination sequences, and optionally 5′-flanking non-transcribed sequences. DNA sequences derived from the SV40 or CMV viral genomes, for example SV40 or CMV origin, early promoters, enhancers, and polyadenylation sites may be used to provide the required non-transcribed genetic elements.  
       [0061] A recombinant expression vector used in the invention advantageously also comprises an untranscribed polynucleotide region located at the 3′end of the coding sequence (ORF), this 3′-UTR (untranslated region) polynucleotide being useful for stabilizing the corresponding mRNA or for increasing the expression rate of the vector insert if this 3′-UTR harbours regulation signal elements such as enhancer sequences.  
       [0062] Suitable promoter regions used in the expression vectors are chosen taking into account the host cell in which the nucleic acid sequence is to be expressed. A suitable promoter may be heterologous with respect to the nucleic acid sequence for which it controls the expression, or alternatively can be endogenous to the native polynucleotide containing the coding sequence to be expressed. Additionally, the promoter is generally heterologous with respect to the recombinant vector sequences within which the construct promoter/coding sequence has been inserted. Preferred promoters are the LacI, LacZ, T3 or T7 bacteriophage RNA polymerase promoters, the lambda PR, PL and Trp promoters (see EP-0 036 776), the polyhedrin promoter, or the p10 protein promoter from baculovirus (kit Novagen; Smith et al., (1983); O&#39;Reilly et al. (1992)).  
       [0063] Preferred selectable marker genes contained in the expression recombinant vectors used in the invention for selection of transformed host cells are preferably dehydrofolate reductase or neomycin resistance for eukaryotic cell culture, TRP1 for  S. cerevisiae  or tetracycline, rifampicin or ampicillin resistance in  E. coli , or Levamsaccharase for Mycobacteria, this latter marker being a negative selection marker.  
       [0064] Preferred bacterial vectors are listed hereafter as illustrative but not limitative examples: pQE70, pQE60, pQE-9 (Quiagen), pD10, phagescript, psiX174, p.Bluescript SK, pNH8A, pNH16A, pNH18A, pNH46A (Stratagene); pKK223-3, pKK233-3, pDR540, pRIT5 (Pharmacia); pWLNEO, pSV2CAT, pOG44, pXT1, pSG (Stratagene); pSVK3, pBPV, pMSG, pSVL (Pharmacia); pQE-30 (QIA express).  
       [0065] Preferred bacteriophage recombinant vectors of the invention are P1 bacteriophage vectors such as described by Sternberg N. L. (1992;1994).  
       [0066] A suitable vector for the expression of any of the pain associated polypeptides used in the invention or fragments thereof, is a baculovirus vector that can be propagated in insect cells and in insect cell-lines. A specific suitable host vector system is the pVL 1392/1393 baculovirus transfer vector (Pharmingen) that is used to transfect the SF9 cell line (ATCC N o  CRL 1711) that is derived from  Spodoptera frugiperda.    
       [0067] The recombinant expression vectors of in the invention may also be derived from an adenovirus. Suitable adenoviruses are described by Feldman and Steig (1996) or Ohno et al. (1994). Another preferred recombinant adenovirus is the human adenovirus type two or five (Ad 2 or Ad 5) or an adenovirus of animal origin (Patent Application WO 94/26914)  
       [0068] Particularly preferred retrovirus for the preparation or construction of retroviral in vitro or in vivo gene delivery vehicles include retroviruses selected from the group consisting of Mink-Cell Focus Inducing Virus, Murine Sarcoma Virus, and Ross Sarcoma Virus. Other preferred retroviral vectors are those described in Roth et al. (1996), in PCT Application WO 93/25234, in PCT Application WO 94/06920, and also in Roux et al. (1989), Julan et al.(1992) and Nada et al. (1991).  
       [0069] Yet, another viral vector system that is contemplated is the Adeno Associated Viruses (AAV) such as those described by Flotte et al. (1992), Samulski et al. (1989) and McLaughlin et al. (1996).  
       [0070] Host Cells Expressing Pain Associated Polypeptides  
       [0071] Host cells that endogenously express pain associated polypeptides or have been transformed or transfected with one of the nucleic acid ssequences described herein, or with one of the recombinant vector described above, particularly a recombinant expression vector, can be used in the present invention. Also included are host cells that are transformed (prokaryotic cells) or are transfected (eukaryotic cells) with a recombinant vector such as one of those described above.  
       [0072] Preferred host cells used as recipients for the expression vectors used in the invention are the following:  
       [0073] (a) prokaryotic host cells:  Escherichia coli , strains. (i.e. DH5-α, strain)  Bacillus subtilis, Salmonella typhimurium  and strains from species like Pseudomonas, Streptomyces and Staphylococcus for the expression of up and down-regulated nucleic acid sequence modulated by pain, characterized by having at least 80% sequence identity with any of the nucleic acid sequences of Tables I-VI. Plasmid propagation in these host cells can provide plasmids for transfecting other cells.  
       [0074] (b) eukaryotic host cells: HeLa cells (ATCC N o  CCL2; N o  CCL2.1; N o  CCL2.2), Cv 1 cells (ATCC N o  CCL70), COS cells (ATCC N o  CRL 1650; N o  CRL 1651), Sf-9 cells (ATCC N o  CRL 1711), C127 cells (ATCC N o  CRL-1804), 3T3 cells (ATCC N o  CRL-6361), CHO cells (ATCC N o  CCL-61), human kidney 293 cells (ATCC N o  45504; N o  CRL-1573), BHK (ECACC N o 84100 501; N o 84111301), PC12 (ATCC N o  CRL-1721), NT2, SHSY5Y (ATCC N o  CRL-2266), NG108 (ECACC N o 88112302) and F11, SK-N-SH (ATCC N o  CRL-HTB-11), SK-N-BE(2) (ATCC N o  CRL-2271), IMR-32 (ATCC N o  CCL-127). A preferred system to which the nucleic acids of the invention can be expressed are neuronal cell lines such as PC12, NT2, SHSY5Y, NG108 and F11, SK-N-SH, SK-N-BE(2), IMR-32 cell lines, COS cells, 3T3 cells, HeLa cells, 292 cells and CHO cells. The above cell lines could be used for the expression of any of the nucleic acid sequences of Tables I-VI.  
       [0075] When a nucleic acid sequence of any of Tables I-X is expressed using a neuronal cell line, the sequence can be expressed through an endogenous promoter or native neuronal promoter, or an exogenous promoter. Suitable exogenous promoters include SV40 and CMV and eukaryotic promoters such as the tetracycline promoter. The preferred promoter when pain associated molecules are endogenously expressed is an endogenous promoter. A preferred promoter in a recombinant cell line is the CMV promoter.  
       [0076] In a specific embodiment of the host cells described above, these host cells have also been transfected or transformed with a polynucleotide or a recombinant vector for the expression of a natural ligand of any of the nucleic acid sequences of any of Tables I-VI or a modulator of these expression products.  
       [0077] Proteins, Polypeptides and Fragments  
       [0078] The expression products of the nucleic acid sequences of Tables I-VI or fragment(s) thereof can be prepared using recombinant technology, from cell lines or by chemical synthesis. Recombinant methods, chemical methods or chemical synthetic methods can be used to modify a gene in order to introduce into the gene product, or a fragment of the gene product, features such as recognition tags, cleavage sites or other modifications. For efficient polypeptide production, the endogenous expression system or recombinant expression system should allow the expression products to be expressed in a manner that will allow the production of a functional protein or fragment thereof which can be purified. Preferred cell lines are those that allow high levels of expression of polypeptide or fragments thereof. Such cell lines include cell lines which naturally express the nucleic acid sequence of Tables I-VI or common mammalian cell lines such as CHO cells or COS cells, etc, or more specific neuronal cell lines such as PC12. However, other cell types that are commonly used for recombinant protein production such as insect cells, amphibian cells such as oocytes, yeast and prokaryotic cell lines such as  E. coli  can also be used.  
       [0079] The expression products of Tables I-VI or fragments thereof can be utilized in screens to identify potential therapeutic ligands, either as a purified protein, as a protein chimera such as those produced in phage display, as a cell membrane (lipid or detergent) preparation, or in intact cells.  
       [0080] The invention also relates generally to the use of proteins, peptides and peptide fragments for the development of screening technologies for the identification of molecules for the prevention or treatment of pain, and the development of diagnostic tools for the identification and characterization of pain. These peptides include expression products of the nucleic acid sequences of Tables I-VI and purified or isolated polypeptides or fragments thereof having at least 90%, preferably 95%, more preferably 98% and most preferably 99% sequence identity with the any of the expression products of nucleic acid sequences of Tables I-VI. Expressed peptides and fragments of any of these nucleic acid sequences can be used to develop screening technologies for the identification of novel molecules for the prevention or treatment of pain. These screening technologies could also be used to ascribe new pain therapeutic indications to molecules, which have not previously been ascribed for the prevention or treatment of pain. Furthermore the said expressed peptides and fragments can be used as diagnostic tools and for the development of diagnostic tools.  
       [0081] Screening Methods  
       [0082] As discussed above, we have identified nucleic acid sequences whose expression is regulated by pain, particularly chronic pain and more particularly diabetic pain. The expression products of these nucleic acids can be used for screening ligand molecules for their ability to prevent or treat pain, and particularly, but not exclusively, chronic pain. The main types of screens that can be used are described below. The test compound can be a peptide, protein or chemical entity, either alone or in combination(s), or in a mixture with any substance. The test compound may even represent a library of compounds.  
       [0083] The expression products of any of the nucleic acid sequences of Tables I-VI or fragments thereof can be utilized in a ligand binding screen format, a functional screen format or invivo format. Examples of screening formats are provided.  
       [0084] A) Ligand Binding Screen  
       [0085] In ligand binding screening a test compound with is contacted with an expression product of one of the sequences of Tables I-VI, and the ability of said test compound to interact with said expression product is determined, e.g. the ability of the test compound(s) to bind to the expression product is determined. The expression product can be a part of an intact cell, lipidic preparation or a purified polypeptide(s), optionally attached to a support, such as beads, a column or a plate etc.  
       [0086] Binding of the test compound is preferably performed in the presence of a ligand to allow an assessment of the binding activity of each test compound. The ligand may be contacted with the expression product either before, simultaneously or after the test compound. The ligand should be detectable and/or quantifiable. To achieve this, the ligand can be labelled in a number of ways, for example with a chromophore, radioactive, fluorescent, phosphorescent, enzymatic or antibody label. Methods of labelling are known to those in the art. If the ligand is not directly detectable it should be amenable to detection and quantification by secondary detection, which may employ the above technologies. Alternatively the expression product or fragment thereof can be detectable or quantifiable. This can be achieved in a similar manner to that described above.  
       [0087] Binding of the test compound modifies the interaction of the ligand with its binding site and changes the affinity or binding of the ligand for/to its binding site. The difference between the observed amount of ligand bound relative to the theoretical maximum amount of ligand bound (or to the ligand bound in the absence of a test compound under the same conditions) is a reflection of the binding ability (and optionally the amount and/or affinity) of a test compound to bind the expression product.  
       [0088] Alternatively, the amount of test compound bound to the expression product can be determined by a combination of chromatography and spectroscopy. This can be achieved with technologies such as Biacore (Amersham Pharmacia). The amount of test compound bound to the expression product can also be determined by direct measurement of the change in mass upon compound or ligand binding to the expression product. Alternatively, the expression product, compound or ligand can be fluorescently labelled and the association of expression product with the test compound can be followed by changes in Fluorescence Energy Transfer (FRET).  
       [0089] The invention therefore includes a method of screening for pain alleviating compounds, comprising:  
       [0090] a) contacting a test compound or test compounds in the presence of a ligand with an expression product of any of the nucleic acid sequences of Tables I-VI or with cell expressing at least one copy of the expression product or with a lipidic matrix containing at least one copy of the expression product;  
       [0091] b) determining the binding of the test compound to the expression product, and  
       [0092] c) selecting test compounds on the basis of their binding abilities.  
       [0093] In the above method, the ligand may be added prior to, simultaneously with or after contact of the test compound with the expression product. Non limiting examples and methodology can be gained from the teachings of the  Molecular Probes handbook  and references therein (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA),  Methods in neurotransmitter receptor analysis  (Yamamura H I., Enna, S J., and Kuhar, M J., Raven Press New York, the  Glaxo Pocket Guide to Pharmacology , Dr. Michael Sheehan, Glaxo Group Research Ltd, Ware, Herts SG12 0DP), Bylund D B and Murrin L C (2000, Life Sciences, 67 (24) 2897-911), Owicki J C (2000 , J. biomol Screen  (5) 297-306), Alberts et al (1994 , Molecular Biology of the Cell , 3 rd  Edn, Garland Publication Inc), Butler J E, (2000  Methods  22(1):4-23, Sanberg S A (2000, Curr Opin Biotechnol 11(1) 47-53), and Christopoulos A (1999, biochem Pharmacol 58(5) 735-48).  
       [0094] B) Functional Screening  
       [0095] (a) Kinase Assays  
       [0096] The expression product of any of the nucleic acid sequences of Tables I-VI which encode a kinase, and in particular the nucleic acid sequence listed in Table I, is amenable to screening using kinase assay technology.  
       [0097] Kinases have the ability to add phosphate molecules to specific residues in ligands such as binding peptides in the presence of a substrate such as adenosine triphosphate (ATP). Formation of a complex between the kinase, the ligand and substrate results in the transfer of a phosphate group from the substrate to the ligand. Compounds that modulate the activity of the kinase can be determined with a kinase functional screen. Functional screening for modulators of kinase activity therefore involves contacting one or more a test compounds with an expression product of one of the nucleic acid sequences of Tables I-VI which encodes a kinase, and determining the ability of said test compound to modulate the transfer of a phosphate group from the substrate to the ligand.  
       [0098] The expression product can be part of an intact cell or of a lipidic preparation or it can be a purified polypeptide(s), optionally attached to a support, for example beads, a column, or a plate. Binding is preferably performed in the presence of ligand and substrate to allow an assessment of the binding activity of each test compound.  
       [0099] The ligand should contain a specific kinase recognition sequence and it should not be phosphorylated at its phosphoryation site. The ligand and/or substrate may be contacted with the kinase either before, simultaneously or after the test compound. Optionally the substrate may be labelled with a kinase transferable labelled phosphate. The assay being monitored by the phosphorylation state of the substrate and/or the ligand. The ligand should be such that its phosphorylation state can be determined. An alternative method to do this is to label the ligand with a phosphorylation-state-sensitive molecule. To achieve this, the ligand can be labelled in a number of ways, for example with a chromophore, radioactive, fluorescent, phosphorescent, enzymatic or antibody label. If the ligand is not directly detectable it should be amenable to detection and quantification by secondary detection, which may employ the above technologies. Such technologies are known to those in the art.  
       [0100] Binding of the test compound to the kinase modifies its ability to transfer a phosphate group from the substrate to the ligand. The difference between the observed amount of phosphate transfer relative to the theoretical maximum amount of phosphate transfer is a reflection of the modulatory effect of the test compound. Alternatively, the degree of phosphate transfer can be determined by a combination of chromatography and spectroscopy. The extent of phosphorylation of the ligand peptide or dephosphorylation of the substrate can also be determined by direct measurement. This can be achieved with technologies such as Biacore (Amersham Pharmacia).  
       [0101] The invention also provides a method for screening compounds for the ability to relieve pain, which comprises:  
       [0102] (a) contacting one or more test compounds in the presence of ligand and substrate with an expression product of any of the sequences of Tables I-VI which is a kinase or with a cell containing at least 1 copy of the expression product or with a lipidic matrix containing at least 1 copy of an expression product;  
       [0103] (b) determining the amount of phosphate transfer from the substrate to the ligand; and  
       [0104] (c) selecting test compounds on the basis of their capacity to modulate phosphate transfer.  
       [0105] Optionally ligand, substrate and/or other essential molecules may be added prior to contacting the test compound with expression product of step (a) or after step (a). Non limiting examples and methodology can be gained from the teachings of the  Molecular Probes handbook  and references therein (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA),  Methods in Molecular Biology  2000; 99: 191-201 , Oncogene  2000 20; 19(49): 5690-701, and  FASAB Journal , (10, 6, P55, P1458, 1996, Pocius D Amrein K et al).  
       [0106] b) Receptor Assays  
       [0107] An expression product of any of the nucleic acid sequences of Tables I-VI which encodes a receptor, and in particular any of the nucleic acid sequences listed in Table II, is amenable to screening using receptor assay technology.  
       [0108] Receptors are membrane associated proteins that initiate intracellular signalling upon ligand binding. Therefore, the identification of molecules for the prevention and treatment of pain can be achieved with the use of a ligand binding assay, as outlined above. Such an assay would utilize an endogenous or non-endogenous ligand as a component of the ligand binding assay. The binding of this ligand to the receptor in the presence of one or more test compounds would be measured as described above. Such is the nature of receptors that the assay is usually, but not exclusively performed with a receptor as an intact cell or membraneous preparation.  
       [0109] The invention therefore includes a method of screening for pain alleviating compounds, comprising:  
       [0110] a) contacting a test compound or test compounds in the presence of a ligand with cell expressing at least one copy of the expression product of any of the sequences of Tables I-VI which is a receptor or with a lipidic matrix containing at least one copy of the expression product;  
       [0111] b) determining the binding of the test compound to the expression product, and  
       [0112] c) selecting test compounds on the basis of their binding abilities.  
       [0113] c) Transporter Protein Assays  
       [0114] An expression product of any of the nucleic acid sequences of Tables I-VI which encodes a transporter protein, and in particular any of the nucleic acid sequences listed in Table III, is amenable to screening using transporter protein assay technology. Non limiting examples of technologies and methodologies are given by Carroll F I, et al (1995, Medical Research Review, Sep15 (5) p419-444), Veldhuis J D and Johnson M l (1994, Neurosci. Biobehav Rep., winter 18(4) 605-12), Hediger M A and Nussberger S (1995, Expt Nephrol, July-Aug 3(4) p211-218, Endou H and Kanai Y, (1999, Nippon Yakurigaku Zasshi, Oct. 114 Suppl 1:1p-16p), Olivier B et al (2000, Prog. Drug Res., 54, 59-119), Braun A et al (2000, Eur J Pharm Sci, oct 11, Suppl 2 S51-60) and  Molecular Probes handbook and references therein  (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA).  
       [0115] The main function of transporter proteins is to facilitate the movement of molecules across a cellular membrane. Compounds that modulate the activity of transporter proteins can be determined with a transporter protein functional screen. Functional screening for modulators of transporter proteins comprises contacting at least one test compound with an expression product as aforesaid which is a transporter protein and determining the ability of said test compound to modulate the activity of said transporter protein. The expression product can be part of an intact cell, or lipidic preparation, optionally attached to a support, for example beads, a column or a plate. Binding is preferably performed in the presence of the molecule to be transported, which should only able to pass through a cell membrane or lipidic matrix with the aid of the transporter protein. The molecule to be transported should be able to be followed when it moves into a cell or through a lipidic matrix. Preferably the molecule to be transported is labelled to aid in characterization, e.g. with a chromophore, radioactive, fluorescent, phosphorescent, enzymatic or antibody label. If the molecule to be transported is not directly detectable it should be amenable to detection and quantification by secondary detection, which may employ the above technologies. The molecule to be transported may be contacted with the transporter protein before, simultaneously with or after the test compound. If binding of the test compound to the transporter protein modifies its ability to transport molecules through a membraneous or lipidic matrix, then the difference between the observed amount of transported molecule in a cell/or through a lipidic matrix relative to the theoretical maximum amount is a reflection of the modulatory effect of the test compound.  
       [0116] The invention further provides a method for screening compounds for their ability to relieve pain, comprising  
       [0117] a) contacting at least one test compound in the presence of transporter molecules with a cell containing at least one copy of an expression product of any of the sequences of Tables I-VI which is a transporter protein or with a lipidic matrix containing at least one copy of the expression product;  
       [0118] c) measuring the movement of transported molecules into or from the cell, or across the lipidic matrix; and  
       [0119] d) selecting test compounds on the basis of their ability to modulate the movement of transported molecules.  
       [0120] d) G-Protein Coupled Receptor Protein Assays  
       [0121] An expression product of any of the nucleic acid sequences of Tables I-VI that encodes a G-protein coupled receptor protein, and in particular any of the nucleic acid sequences listed in Table IV, is amenable to screening using G-protein coupled receptor protein assay technology.  
       [0122] G-protein coupled receptor proteins (GPCRs) are membrane associated proteins whose main function is to transduce a signal through a cellular membrane. Upon ligand binding, GPCRs undergo a conformational change that allows complexing of the GPCRs with a G-protein. G-proteins possess a GTP/GDP binding site. The formation of the G-protein/ligand complex allows exchange of GTP for GDP, resulting in a conformational change of the G-protein. This conformational change initiates signal transduction.  
       [0123] Functional screening for modulators of GPCRs comprises contacting at least one test compound with an expression product as aforesaid which is a G-protein coupled receptor protein, and assessing the ability of the test compound(s) to modulate the exchange of GTP for GDP or the modulation of the GPCR signal transduction pathway. The expression product can be part of an intact cell or lipidic preparation, optionally attached to a support, for example beads, a column or a plate. Binding is preferably performed in the presence of a ligand, G-protein and GTP/GDP to allow an assessment of the binding activity of each test compound. Alternatively components of the signaling pathway are also included. In particular, in a preferred embodiment, a labeled GTP is used and the ability of the test compound(s) to modulate the exchange of GTP to GDP is determined. A further optional characteristic of the assay can be the inclusion of a reporter molecule that enables monitoring the regulation of the signaling pathway. The ligand may be contacted with the GPCR before, simultaneously with, or after the test compound. Binding of the test compound to the GPCR modifies its ability to modulate the exchange of GTP for GDP and hence the modulation of signal transduction. The difference between the observed amount of GTP exchanged for GDP relative to the theoretical maximum amount of GTP is a reflection of the modulatory effect of the test compound. Likewise the relative activities of signal transduction reporter molecules are also a reflection of the modulatory effect of the test compound. Non limiting examples of technologies and methodologies can be found in  Molecular Probes handbook  and references therein (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA),  Glaxo Pocket Guide to Pharmacology , (Michael Sheehan, Pharmacology Division staff, Glaxo Group Research Ltd., Ware, Herts SC12 0DP) and Xing et al (2000, J. Recept. Signal. Transduct. Res. 20(40 189-210).  
       [0124] The invention provides a method of screening compounds for their ability to relieve pain, comprising:  
       [0125] a) contacting at least one test compound in the presence of a ligand, GTP/GDP, G-protein with a cell containing at least one copy of an expression product of a sequence of Tables I-VI as aforesaid which is a G-protein coupled receptor protein or with a lipidic matrix containing at least one copy of the expression product;  
       [0126] b) measuring the exchange of GTP for GDP, and  
       [0127] c) selecting test compounds on the basis of their ability to modulate said exchange.  
       [0128] In the above method, the ligand, GTP/GDP, G-protein and other essential molecules can be added before, simultaneously with or after the contacting of the test compound(s) with the cell line or lipidic matrix in step (a).  
       [0129] e) DNA-Binding Protein Assays  
       [0130] An expression product of any of the nucleic acid sequences of Tables I-VI that encodes a DNA-binding protein, and in particular any of the nucleic acid sequences listed in Table V, is amenable to screening using DNA-binding protein assay technology.  
       [0131] DNA binding proteins are proteins that are able to complex with DNA. The complexing of the DNA binding protein with the DNA in some instances requires a specific nucleic acid sequence. Screens can be developed in a similar manner to ligand binding screens as previously indicated and will utilise DNA as the ligand. DNA-binding protein assays can be carried using similar principles described in ligand binding assays as described above. Non limiting examples of methodology and technology can be found in the teachings of Haukanes B I and Kvam C (Biotechnology, 1993 Jan 11 60-63), Alberts B et al (Molecular Biology of the Cell, 1994, 3 rd  Edn., Garland Publications Inc, Kirigiti P and Machida C A (2000 Methods Mol Biol, 126, 431-51) and  Molecular Probes handbook  and references therein (Molecular Probes, Inc., 4849 Pitchford Ave., Eugene, USA).  
       [0132] The invention therefore includes a method of screening for pain alleviating compounds, comprising:  
       [0133] a) contacting a test compound or test compounds in the presence of a plurality of nucleic acid sequences with an expression product of any of the nucleic acid sequences of Tables I-VI which is a DNA binding protein or with cells expressing at least one copy of the expression product or with a lipidic matrix containing at least one copy of the expression product;  
       [0134] b) determining the binding of the test compound to the expression product, and  
       [0135] c) selecting test compounds on the basis of their binding abilities.  
       [0136] In the above method, the plurality of nucleic acid sequence may be added prior to, simultaneously with or after contact of the test compound with the expression product.  
       [0137] f) Assays Using other Enzymes  
       [0138] Expression products of any of the nucleic acid sequences of Tables I-VI that encode other enzymes, e.g. ligases, lyases, oxidoreductases, transferases and hydrolases, and in particular any of the nucleic acid sequences listed in Table VI, is amenable to screening using appropriate assay technology.  
       [0139] Each class of enzyme has a defined function. Ligases have the property of being able to splice molecules together. This is achieved with the conversion of ATP substrate to AMP. Therefore, the activity of a ligase can be followed by monitoring the conversion of ATP to AMP. Such technologies are known to those in the art. Non limiting examples and methodologies are illustrated by Ghee. T Tan et al (1996,Biochem J. 314, 993-1000, Yang S W et al (1992, 15: 89(6) 2227-31 and refrences therein, and in  Molecular Probes handbook  and references therein (Molecular Probes, Inc., 4849 Pitchford Ave, Eugene, USA).  
       [0140] The invention also provides a methods of screening for pain alleviating compounds, comprising;  
       [0141] a) contacting one or more test compounds in the presence of ATP with an expression product of any of the sequences of Tables I-VI which is a ligase or with a cell expressing at least 1 copy of a expression product or with a lipidic matrix containing at least 1 copy of an expression product which is a ligase;  
       [0142] b) determining the amount of ATP converted to AMP, and  
       [0143] c) selecting test compounds on the basis of their ability to modulate said conversion.  
       [0144] Lyases are enzymes which catalyse the cleavage of by reactions other than hydrolysis. These enzymes can be grouped into seven groups according to type of bond cleaved. These groups are carbon-carbon lyases (E.C. No 4.1), carbon-oxygene lyases (E.C. No 4.2), carbon-nitrogen lyases (E.C. No 4.3), carbon-sulphur lyase (E.C. No 4.4) carbon-halide lyases (E.C. No 4.5), phosphorous-oxygene lyases (E.C. No 4.6) and other lyases (E.C. No 4.99) (Analytical Biochemistry 3 rd  Edn, David J. Holme and Hazel Peck, Longman press). The enzyme commission number (E.C.) of the International Union of Biochemistry relates to the type of reaction catalysed by the enzyme. Further teachings on how to develop assays and screens for lyases can be obtained from Methods in Enzymology (Academic Press).  
       [0145] Oxidoreductases are enzymes that catalyse the transfer of hydrogen or oxygen atoms or electrons. These enzymes can by sub-grouped into twenty categories according to their specific mode of action. These groups are oxidoreductases acting on the CH—OH group of donors (E.C. No1.1), oxidoreductases acting on the aldehyde or oxo group of donors (E.C. No 1.2), oxidoreductases acting on the CH—CH group of the donor (E.C. No 1.3), oxidoreductases acting on the CH—NH2 group of donors (E.C. 1.4), oxidoreductases acting on the CH—NH group of donor (E.C. 1.5), oxidoreductases acting on the NADH or NADPH (E.C. No 1.6), oxidoreductases acting on other nitrogen compounds as donors (E.C. No 1.7), oxidoreductases acting on a sulphur group of donors (E.C. No 1.8), oxidoreductases acting on a haem group of donors (E.C. No1.9), oxidoreductases acting on diphenols and related substances as donors (E.C. 1.10), oxidoreductases acting on hydrogen peroxide as acceptor (E.C. No 1.11), oxidoreductases acting on hydrogen as donor (E.C. No 1.12), oxidoreductases acting on single doners with incorporation of molecular oxygen (E.C. No 1.13), oxidoreductases acting on paired donors with incorporation of molecular oxygen (E.C. No 1.14), oxidoreductases acting on superoxide radicals as acceptors (E.C. 1.15), oxidoreductases oxidizing metal ions (E.C. No 1.16), oxidoreductases acting on —CH2 groups (E.C. No 1.17), oxidoreductases acting on reduced ferredoxin as donor (E.C. No 1.18), oxidoreductases acting on reduced flavodoxin as donor (E.C. No 1.19) and other oxidoreductases (E.C. No 1.97) (Analytical Biochemistry 3 rd  Edn, David J. Holme and Hazel Peck, Longman Press). The enzyme commission number (E.C.) of the International Union of Biochemistry relates to the type of reaction catalysed by the enzyme. Further teachings on how to develop assays and screens for oxidoreductases can be obtained from Methods in Enzymology (Academic Press) with special reference to volume 249.  
       [0146] Transferases are enzymes that catalyse the transfer of specific groups. They are classified into eight sub groups according to function, transferring one-carbon group (E.C. No 2.1), Transfering aldehyde or ketonic residues (E.C. No 2.2), Acetyltransferases (E.C. 2.3), glycosyltransferases (E.C. No 2.4), transferring alkyl or aryl groups other than methyl groups (E.C. No 2.5), transferring nitrogeneous groups (E.C. No 2.6), transferring phosphorous-containing groups (E.C. No 2.7) and transferring sulphur-containing groups (E.C. No 2.8) (Analytical Biochemistry 3 rd  Edn, David J. Holme and Hazel Peck, Longman Press). The enzyme commission number (E.C.) of the International Union of Biochemistry relates to the type of reaction catalysed by the enzyme. Further teachings on how to develop assays and screens for transferases can be obtained from Methods in Enzymology (Academic Press).  
       [0147] Hydrolases are enzymes that catalyse hydrolytic reactions and are sub-grouped into eleven classes according to the type of reaction they carry out. Hydrolases acting on ester bonds (E.C. No 3.1), hydrolases acting on glycosyl compounds (E.C. No 3.2), hydrolases acting on ether bonds (E.C. No 3.3), hydrolases acting on peptide bonds (E.C. No 3.4), hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (E.C. No 3.5), hydrolases acting on acid anhydrides (E.C. No 3.6), hydrolases acting on acid anhydrides (E.C. No 3.6), hydrolases acting on carbon-carbon bonds (E.C. No 3.7), hydrolases acting on halide bonds (E.C. No 3.8), hydrolases acting on phosphorous-nitrogen bonds (E.C. No 3.9), hydrolases acting on sulphur-nitrogen bonds (E.C. No 3.10) and hydrolases acting on carbon-phosphorous bonds (Analytical Biochemistry 3 rd  Edn, David J. Holme and Hazel Peck, Longman Press). The enzyme commission number (E.C.) of the International Union of Biochemistry relates to the type of reaction catalysed by the enzyme. Further teachings on how to develop assays and screens for hydrolases can be obtained from Methods in Enzymology (Academic Press) with special reference to volume 249.  
       [0148] C) In Vivo Functional Screen  
       [0149] Any of the nucleotide sequences described in Tables I-VI or homologues thereof may be inserted by means of an appropriate vector into the genome of a lower vertebrate or of an invertebrate animal or may be inactivated or down regulated in the genome of said animal. The resulting genetically modified animal may be used for screening compounds for effectiveness in the regulation of pain. The invertebrate may, for example, be a nematode e.g.  Caenorhabditis elegans , which is a favourable organism for the study of response to noxious stimuli. Its genome sequence has been determined, see  Science , 282, 2012 (1998), it can be bred and handled with the speed of a micro-organism (it is a self-fertilizing hermaphrodite) and can therefore be used in a high throughput screening format (WO 00/63424, WO 00/63425, WO 00/63426 and WO 00/63427), and it offers a full set of organ systems, including a simple nervous system and contains many similarly functioning genes and signaling pathways to mammals. A thermal avoidance model based on a reflexive withdrawal reaction to an acute heat stimulus has been described by Wittenburg et al,  Proc. Natl. Acad. Sci. USA , 96, 10477-10482 (1999), and allows the screening of compounds for the treatment of pain with the modulation of pain sensation as an endpoint.  
       [0150] The genome of  C. elegans  can be manipulated using homologous recombination technology which allows direct replacement of nucleic acids encoding  C. elegans  with their identified mammalian counterpart. Replacement of these nucleic acids with those nucleic acids outlined above would allow for the direct screening of test compound(s) with their expression products. Any of the pain-related genes described above may be ligated into a plasmid and introduced into oocytes of the worm by microinjection to produce germline transformants. Successful plasmid injection into  C. elegans  and expression of inserted sequences has been reported by Devgen B. V., Ghent, Belgium. It is also possible to produce by routine methods worms in which the target sequences are down-regulated or not expressed (knock-out worms). Further non limiting examples of methodology and technology can be found in the teachings of Hazendonk et al (1997, Nat genet. 17(1) 119-21), Alberts et al, (1994, Molecular Biology of the Cell 3 rd  Ed. Garland Publishing Inc,  Caenorhabditis elegans  is anatomically and genetically simple), Broverman S et al, (1993, PNAS USA 15;90(10) 4359-63) and Mello et al (1991, 10(12)3959-70).  
       [0151] A further method for screening compounds for ability to modify response to pain, e.g. relieve pain, comprises:  
       [0152] (a) contacting one or more test compounds with at least one  C elegans  containing at least one copy of a sequence as set out above;  
       [0153] (b) subjecting the  C. elegans  to a nociceptive stimulus;  
       [0154] (c) observing the response of the  C. elegans  to said stimulus; and  
       [0155] d) selecting test compounds on the basis of their ability to modify the response of  C. elegans  to said stimulus.  
       [0156] Diagnostic Tools and Kits  
       [0157] Affinity Peptides, Ligands and Substrates  
       [0158] Pain associated polypeptides and fragments thereof can be detected at the tissue and cellular levels with the use of affinity peptides, ligands and substrates, which will enable a skilled person to define more precisely a patient&#39;s ailment and help in the prescription of a medicament. Such affinity peptides are characterized in that firstly they are able to bind specifically to a pain associated polypeptide, and secondly that they are capable of being detected. Such peptides can take the form of a peptide or polypeptide for example an antibody domain or fragment, or a peptide/polypeptide ligand or substrate, or a polypeptide complex such as an antibody.  
       [0159] The preparation of such peptides and polypeptides are known to those in the art. Antibodies, these may be polyclonal or monoclonal, and include antibodies derived from immunized animals or from hybridomas, or derivatives thereof such as humanized antibodies, Fab or F(ab′)2 antibody fragments or any other antibody fragment retaining the antigen binding specificity.  
       [0160] Antibodies directed against pain-associated gene product molecules may be produced according to conventional techniques, including the immunization of a suitable mammal with the peptides or polypeptides or fragment thereof. Polyclonal antibodies can be obtained directly from the serum of immunized animals. Monoclonal antibodies are usually produced from hybridomas, resulting from a fusion between splenocytes of immunized animals and an immortalized cell line (such as a myeloma). Fragments of said antibodies can be produced by protease cleavage, according to known techniques. Single chain antibodies can be prepared according to the techniques described in U.S. Pat. No. 4,946,778. Detection of these affinity peptides could be achieved by labeling. technologies which allow detection of peptides, such as enzymatic labeling, fluorescence labeling or radio-labeling are well known to those in the art. Optionally these affinity peptides, ligands and substrates could themselves be detected with the use of a molecule that has specific affinity to the peptides, ligands and substrates and is itself labeled.  
       [0161] The invention further provides a kit comprising;  
       [0162] (a) affinity peptide and/or ligand and/or substrate for an expression product of a gene sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain; and  
       [0163] (b) a defined quantity of an expression product of a gene sequence that is down-regulated in the spinal cord of a mammal both in response to first and second models of neuropathic or central sensitization pain,  
       [0164] for simultaneous, separate or sequential use in detecting and/or quantifying an expression product of a gene sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain.  
       [0165] Complimentary Nucleic Acids  
       [0166] Pain associated nucleic acid sequences can be characterized at the tissue and cellular levels with the use of complimentary nucleic acid sequences. Detection of the level of expression of pain associated nucleic acid sequences can help in the prognosis of a pain condition and the prescription of a medicament. These complimentary nucleic acids are characterized in that they can hybridize to a pain associated nucleic acid sequence and their presence can be detected through various techniques. Such techniques are known to those in the art and may include detection by polymerase chain reaction or detection by labeling of complimentary nucleic acid sequences by enzymatic labeling, affinity labeling fluorescent labeling or radio labeling. Complimentary strand nucleic acid sequences of this invention are 10 to 50 bases long, more preferably 15 to 50 bases long and most preferably 15 to 30 bases long, and hybridize to the coding sequence of the nucleic acid sequence.  
       [0167] A further aspect of this invention is a kit that comprises:  
       [0168] (a) nucleic acid sequences capable of hybridization to a nucleic acid sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain; and  
       [0169] (b) a defined quantity of one or more nucleic acid sequences capable of hybridization to a nucleic acid sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain, for simultaneous, separate or sequential use in detecting and/or quantifying a gene sequence that is down-regulated in the spinal cord of a mammal in response to first and second models of neuropathic or central sensitization pain.  
       [0170] Identification and Validation  
       [0171] Subtractive hybridization enables the identification of nucleic acid sequences whose expression profiles are modified by a stimulus. Upon stimulation of a system (in the case of this invention a nociceptive stimulus on an animal model) all observed changes in the level of nucleic acid sequence expression are due to the reaction of the system to the stimulus. Characterization of these changes in expression by way of identification of nucleic acid sequence and level of expression is both identification and validation.  
       [0172] The inventors have developed a four step process which allows for the simultaneous identification and validation of nucleic acid sequences whose expression are regulated by a pain stimulus, preferably a chronic pain stimulus, and more preferably a diabetic pain stimulus. This process may comprise the following steps:  
       [0173] (a) induction of a nociceptive stimulus in test animals;  
       [0174] (b) extraction of nucleic acids from specific neuronal tissue of test and control animals;  
       [0175] (c) selective amplification of differentially expressed nucleic acid sequences; and  
       [0176] (d) identification and characterization of differentially expressed gene products that are modulated by a nociceptive stimulus.  
       [0177] The above process is described in more detail below.  
       [0178] (a) Induction of Nociceptive Stimulus  
       [0179] The effect of the selected nociceptive stimulus on the test animal needs to be confirmable. The test subjects are therefore a species that has a “developed” nervous system, preferably similar to that of humans, most preferably rats or mice. Advantageously, the nociceptive stimulus is analogous to known pain paradigms in humans. One such paradigm of pain is the pain associated with diabetes, which can be induced in rodents with the use of streptozotocin (STZ). The present application requires the sequences to be down-regulated in two pain models which may be, but are not limited to models of neuropathic pain and/or central sensitization, and in which diabetic pain provides the first model and mechanical damage e.g. to a nerve leading into the spine can provide an appropriate second model.  
       [0180] Streptozotocin (STZ) induces hyperglycemia and Type 1 diabetes mellitus in rats. In particular, STZ contains a glucose analogue that allows it to be taken up by the glucose transporter 2 present on the surface of pancreatic β cells, the site of insulin synthesis. Once inside the cell, STZ causes a reduction in the level of nicotinamide adenine dinucleotide (NAD + ). The decrease in NAD +  levels eventually leads to necrosis of the pancreatic β cell, causing a reduction in insulin levels and then diabetes, leading to neuropathy (diabetic) and neuropathic pain (R. B. Weiss,  Cancer Treat. Rep ., 66, 427-438 1982, Guy et al,  Diabetologica , 28, 131-137 1985; Ziegler et al,  Pain , 34, 1-10 1988; Archer et al,  J. Neural. Neurosurgeon. Psychiatry , 46, 491-499 1983). The diabetic rat model has been shown to be a reliable model of hyperalgesia. We have used an STZ-induced diabetic rat model to create a state of hyperlagesia that can be compared with control animals (Courteix et al,  Pain , 53, 81-88 1993).  
       [0181] Three models of neuropathic and/or central sensitization pain in rats, which involve nerve injury, may be used, see Ralston, DD (1998) Present models of neuropathic pain.  Pain Rev . 5: 83-100. The injuries are caused by (1) loosely tying four chromic gut sutures around the sciatic nerve (CCI model developed by Bennett, G J and Y -K Xie, A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man,  Pain  33: 87-107 (1988), (2) tightly ligating one third to one half of the fibers in the sciatic nerve (model developed by Seltzer, Z, R Dubner, Y Shire, A novel behavioral model of neuropathic pain disorders produced in rats by partial sciatic nerve injury,  Pain  43: 205-218, 1990), and (3) tightly ligating the dorsal spinal nerve of a rat at the L5 or L5 and L6 levels (L5 model developed by Kim, S H and J M Chung, An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat,  Pain  50:355-363, 1992).  
       [0182] (b) Extraction of Nucleic Acids from Neuronal Tissue of Test and Control Animals  
       [0183] The inventors have determined that RNA extraction of whole spinal cord nervous tissue would provide a way of identifying nucleic acid sequences whose expression in spinal tissue is modulated by streptozotocin induced diabetes or by a mechanical nerve damage model for neuropathic and/or central sensitization pain e.g. CCI. Test (subjected to the nociceptive stimulus) and control animals were sacrificed, and the tissue to be studied e.g. neural tissue separated. Techniques for so doing vary widely from animal to animal and will be familiar to skilled persons.  
       [0184] A cDNA library can be prepared from total RNA extracted from neural tissue of the test and control animals. Where possible, however, it is preferred to isolate the mRNA from the total RNA of the test and control animals, by affinity chromatography on oligo (dT)-cellulose, and then reverse transcribe the mRNA from the test and control animals to give test and control cDNA. Converting mRNA from the test and control animals to corresponding cDNA may be carried out by any suitable reverse transcription method, e.g. a method as described by Gubler &amp; Hoffman,  Gene , 25, 263-269 (1983). If desired a proprietary kit may be used e.g. the CapFinder PCR cDNA Library Construction Kit (Life Technologies) which is based on long-distance PCR and permits the construction of cDNA libraries from nanograms of total RNA.  
       [0185] (c) Selective Amplification of Differentially Expressed Nucleic Acids  
       [0186] The reverse transcribed cDNA of the test and control animals is subjected to subtractive hybridisation and amplification so that differentially expressed sequences become selectively amplified and commonly expressed sequences become suppressed, so as to over-produce DNA associated with said nociceptic stimulus. A wide range of subtractive hybridisation methods can be used, but the preferred method is so-called suppression subtractive hybridisation, see U.S. Pat. No. 5,565,340 and Diatchenko et al,  Proc. Nat. Acad. Sci. USA , 93, 6025-6030 (1996), the disclosures of which are herein incorporated by reference. Kits for carrying out this method are available from CLONTECH Laboratories, Inc.  
       [0187] (d) Cloning and Sequencing the Differentially Expressed cDNA  
       [0188] The differentially expressed cDNA is ligated into a cloning vector, after which cells of  E. coli  are transformed with the vector and cultured. Positive clones are selected and lysed to release plasmids containing the cDNA insert. The plasmids are primed using forward and reverse primers to either side of the cloning site and the cDNA insert is sequenced. Vector and adaptor sequences are then removed from the output data from the sequencer, leaving only the nucleotide sequence of the differentially expressed gene. The sequence is then checked against data held in a database for homology to known nucleotide sequences and genes, including expressed sequence tags (ESTs) and coding sequences for proteins.  
       [0189] (e) Validation of the Above Method  
       [0190] The importance of the sequences that we have identified in pain is confirmed by the fact that genes have been identified using this method that represent nucleic acid sequences which have previously been implicated in pain, including Calmodulin (pRCM1, Genebank X13933), Enkephalin (Genebank Y07503) and Neurotensin receptor type 2 (Genebank X97121).  
       [0191] The inventors have identified nucleic acid sequences of the MAP kinase pathway, a previously non pain-associated biological pathway. The inventors have subsequently shown that intra-spinal injection of a MEK inhibitor (MEK is part of the MAP kinase pathway) produces a powerful inhibition of pain (Patent application No. U.S. 60/144292). Subsequently, it has been shown that the MAP kinase is also implicated in acute inflammatory pain (Woolf et al, Nature Neuroscience 1999).  
       [0192] The invention will now be further described in the following Example. 
     
    
    
     EXAMPLE  
     [0193] Induction of Diabetes  
     [0194] Diabetes was induced in adult (150-200 g) male Sprague-Dawley rats (n=6) as described by Courteix et al (supra). Animals were injected intraperitoneally with streptozotocin (STZ)(50 mg/kg) dissolved in distilled water. Control or sham animals (age-matched animals, n=6) were injected with distilled water only.  
     [0195] Chronic Constrictive Injury (CCI)  
     [0196] Rats were anaesthetized with i.p. sodium phenobarbital, after which the common left sciatic nerve was exposed at the level of the middle of the thigh by blunt dissection through the biceps femoris and proximal to the sciatic trifurcation. Four ligatures (4.0 braided silk) were tied loosely around it with about 1 mm spacing. The muscle was closed in layers and two wound clips were applied to close the skin incision. The wound was then covered with topical antibiotics.  
     [0197] Nociceptive Testing  
     [0198] Static allodynia (a form of hyperlagesia) was measured using a method described by Chaplan let al, “Quantitative assessment of tactile allodinya in the rat paw”,  J. Neurosci. Methods , 53, 55-63 (1994). A series of von Frey filaments of different buckling weight (i.e. the load required for the filament to bend) were applied to the plantar surface of the right hand paw. The starting filament had a buckling weight of 20 g. Lifting of the paw was taken to be a positive result, in which case a filament with the next lowest buckling weight was used for the next measurement. The test was continued until a filament was found for which there was an absence of response for longer than 5 seconds whereas a re-test with the next heaviest filament gave a positive response. Animals were considered hyperalgesic if their thresholds were found to be &lt;4 g of those of comparable untreated rats, see Calcutt &amp; Chaplan, “Spinal pharmacology of tactile allodynia in diabetic rats”,  British J. Pharmacol , 122, 1478-1482 (1997).  
     [0199] Tissue Extraction  
     [0200] STZ-treated, CCI-treated and control animals were anaesthetized with 4% halothane and perfused with ice-cold 0.9% saline containing 1% citric acid (pH adjusted to 7.4 with NaOH). The animals were decapitated and the lumbar spinal cord exposed. A 2-centimetre length of spinal cord ending at L6 (lumbar-6 forward) was removed from the spinal column. Attached dorsal root ganglia and contaminating spinal connective tissues were removed. The spinal cord tissue was snap frozen in dry ice and isopentane. In the experiments that follow, procedures on the streptozocin-treated and control groups of animals are disclosed. It will be understood that for identification of CCI-treated animals the same experiments are performed, but using tissues from the CCI-treated animals and from control animals.  
     [0201] Total RNA Extraction  
     [0202] Total RNA was extracted from the pooled male rat tissues of the streptozocin-treated and control groups using the TRIZOL Reagent Kit (Life Technologies). Briefly, tissue samples were homogenised fully using a Polytron homogenizer in 1 ml of TRIZOL reagent per 50-100 mg of tissue. Homogenized samples were then incubated at room temperature for 5 minutes and phase separated using 0.2 ml chloroform per 1 ml TRIZOL reagent followed by centrifugation at 3,000 g. The aqueous phase was transferred to a fresh tube and the RNA precipitated with an equal volume of isopropyl alcohol and followed by centrifugation at 10,000 g. The RNA pellet was washed in 75% ethanol and re-centrifuged. The pellet was then air dried and re-suspended in water.  
     [0203] mRNA Extraction  
     [0204] In contrast to ribosomal RNA and transfer RNA, the vast majority of mRNAs of mammalian cells carry tracts of poly(A + ) at their 3′ termini. mRNAs can therefore be separated from the bulk of cellular RNA by affinity chromatography on oligo (dT)-cellulose. mRNA was extracted from Total RNA using the MESSAGEMAKER Kit (Life Technologies) in which mRNA (previously heated to 65° C. in order to disrupt secondary structures and so expose the poly (A + ) tails) was bound to oligo(dT) cellulose under high salt concentrations (0.5M NaCl) in a filter syringe. Unbound RNA was then washed away and the poly(A + ) mRNA eluted in distilled water. A tenth of the volume of 7.5 M Ammonium Acetate, 50 μg of glycogen/ml mRNA sample and 2 volumes of absolute alcohol were then added to the samples which were placed at −20° C. overnight. Following precipitation, the mRNA was spun down at 12,000 g for 30 minutes at 4° C. RNAase free water was used to re-suspend the pellets, which were then and stored at −80° C.  
     [0205] PCR Select  
     [0206] The technique used was based on that of the CLONTECH PCR Select Subtraction Kit. The following protocol was performed using STZ-treated lumbar spinal cord Poly A +  RNA as the ‘Tester’ and Sham lumbar spinal cord poly A +  RNA as the ‘Driver’ (Forward Subtraction). A second subtraction experiment using the Sham lumbar spinal cord mRNA as the ‘Tester’ and STZ treated lumbar spinal cord mRNA as the ‘Driver’ (Reverse Subtraction) was performed in parallel using the same reagents and protocol. As a control for both experiments, the subtraction was also carried out using human skeletal muscle mRNA that had been provided by the manufacturer.  
     [0207] First-Strand cDNA Synthesis  
     [0208] 2 μg of PolyA +  RNA and 1 μl of cDNA synthesis primer (10 μM) were combined in a 0.5 ml Eppendorf tube and sterile H 2 O was added where necessary to achieve a final volume of 5 μl. The contents were mixed gently and incubated in a thermal cycler at 70° C. for 2 min. The tubes were then cooled on ice for two minutes, after which 2 μl of 5×First-strand buffer, 1 μl of dNTP mix (10 mM each), sterile H 2 O and 1 μl of AMV reverse transcriptase (20 units/μl) was also added. The tubes were then placed at 42° C. for 1.5 hr in an air incubator. First-strand cDNA synthesis was terminated by placing the tubes on ice. (the human skeletal muscle cDNA produced by this step was used as the ‘control driver’ in later steps).  
     [0209] Second-Strand cDNA Synthesis  
     [0210] 48.4 μl of Sterile H 2 O, 16.0 μl of 5×Second-strand buffer, 1.6 μl of dNTP mix (10 mM) and 4.0 μl of 20×Second-strand enzyme cocktail were added to each of the first-strand synthesis reaction tubes. The contents were then mixed and incubated at 16° C. in a thermal cycler for 2 hr. 6 units (2 μl) of T4 DNA polymerase was then introduced and the tubes were incubated for a further 30 min at 16° C. In order to terminate second-strand synthesis, 4 μl of 20×EDTA/glycogen mix was added. A phenol:chloroform extraction was then carried out using the following protocol:-  
     [0211] 100 μl of phenol:chloroform:isoamyl alcohol (25:24:1) was added to the tubes which were then vortexed thoroughly and centrifuged at 14,000 rpm for 10 min at room temperature. The top aqueous layer was removed and placed in a fresh tube. 100 μl of chloroform:isoamyl alcohol (24:1) was then added to the aqueous layer and the tubes were again vortexed and centrifuged at 14,000 rpm for 10 min. 40 μl of 4 M NH 4 OAc and 300 μl of 95% ethanol were then added and the tubes centrifuged at 14,000 rpm for 20 min. The supernatant was removed carefully, then 500 μl of 80% ethanol was added to the pellet. The tubes were centrifuged at 14,000 rpm for 10 min and the supernatant was removed so that the pellet could be air-dried. The precipitate was then dissolved in 50 μl of H 2 O. 6 μl was transferred to a fresh microcentrifuge tube. The remainder of the sample was stored at −20° C. until needed.  
     [0212] Rsa I Digestion  
     [0213] All products of the above procedures were subjected to a restriction digest, using the restriction endonuclease Rsa I, in order to generate ds cDNA fragments that are short and thus are optimal for subtraction hybridisation due to the standard kinetics of the hybridisation. Also, as Rsa I makes a double stranded cut in the middle of a recognition sequence, ‘blunt ends’ of a known nucleotide sequence are produced allowing ligation of adaptors onto these ends in a later step. The following reagents were added to the 6 μl product of the second hybridisation (see above): 43.5 μl of ds cDNA, 5.0 μl 10×Rsa I restriction buffer and 1.5 μl of Rsa I (10 units/μl). The reaction mixture was incubated at 37° C. for 1.5 hr. 2.5 μl of 20×EDTA/glycogen mix was used to terminate the reaction. A phenol:chloroform extraction was then performed as above (second-strand cDNA synthesis section). The pellet produced was then dissolved in 5.5 μl of H 2 O and stored at −20° C. until needed. The preparation of the experimental ‘Driver’ cDNAs and the control skeletal muscle cDNA was thus completed.  
     [0214] Adaptor Ligation  
     [0215] The adaptors were not ligated to the driver cDNA.  
     [0216] 1 μl of each Rsa I-digested experimental cDNA (from the Rsa I Digestion above) was diluted with 5 μl of sterile water. Preparation of the control skeletal muscle tester cDNA was then undertaken by briefly centrifuging the tube containing control DNA (Hae III-digest of φX174 DNA [3 ng/μl]) and diluting 2 μl of the DNA with 38 μl of sterile water (to 150 ng/ml). 1 μl of control skeletal muscle cDNA (from the Rsa I Digestion) was then mixed with 5 μl of the diluted φX174/Hae III DNA (150 ng/ml) in order to produce the control skeletal muscle tester cDNA.  
     [0217] Preparation of the Adaptor-Ligated Tester cDNA  
     [0218] A ligation master mix was prepared by combining 3 μl of sterile water, 2 μl of 5×ligation buffer and 1 μl T4 DNA ligase (400 units/μl) per reaction. 2 μl of adaptor 1 (10 μM) was then added to 2 μl of the diluted tester cDNA. To this, 6 μl of the ligation master mix was also added. The tube was therefore labeled Tester 1-1. In a separate tube, 2 μl of the adaptor 2R (10 μM) was mixed with 2 μl of the diluted tester cDNA and 6 μl of the master mix. This tube was named Tester 1-2.  
     [0219] 2 μl of Tester 1-1 and 2 μl of Tester 1-2 were then placed into fresh tubes. These would later be used as the unsubtracted tester control. The remainder of the contents of Tester 1-1 and Tester 1-2 tubes were then centrifuged briefly and incubated at 16° C. overnight. The ligation reaction was stopped by adding 1 μl of EDTA/glycogen mix and the samples were heated at 72° C. for 5 min in order to inactivate the ligase. In doing so, preparation of the experimental and control skeletal muscle adaptor-ligated tester cDNAs was complete.  
     [0220] 1 μl from each unsubtracted tester control was then removed and diluted into 1 ml of water. These samples were set aside as they were to be used later for PCR (see below). All of the samples were stored at −20° C.  
     [0221] Analysis of Ligation Efficiency  
     [0222] 1 μl of each ligated cDNA was diluted into 200 μl of water and the following reagents were then combined in four separate tubes:  
                                                  Tube:                                             Component   1   2   3   4                       Tester 1-1 (ligated to Adaptor 1)   1   1   —   —           Tester 1-2 (ligated to Adaptor 2R)   —   —   1   1           G3PDH 3′ primer (10 μM)   1   1   1   1           G3PDH 5′ primer (10 μM)   —   1   —   1           PCR primer 1 (10 μM)   1   —   1   —           Total volume μl   3   3   3   3                      
 
     [0223] A master mix for all of the reaction tubes plus one additional tube was made up by adding 18.5 μl of sterile H 2 O, 2.5 μl of 10×PCR reaction buffer, 0.5 μl of dNTP mix (10 mM), and 0.5 μl of 50×Advantage cDNA Polymerase Mix, per reaction, into a fresh tube. 22 μl of this master mix was then aliquotted into each of the 4 reaction tubes prepared above. The contents of the tubes were overlaid with 50 μl of mineral oil. The reaction mix was incubated in a thermal cycler at 75° C. for 5 min in order to extend the adaptors. The following protocol was then carried out immediately in a thermal cycler (Perkin-Elmer GeneAmp PCR Systems 2400): 94° C. for 30 sec (1 cycle), 94° C. 10 sec, 65° C. 30 sec and then 68° C. 2.5 min (25 cycles)  
     [0224] First Hybridisation  
     [0225] 1.5 μl of the Adaptor 1-ligated Tester 1-1 was combined with 1.5 μl of the Rsa I-digested driver cDNA, prepared earlier and 1 μl of 4×Hybridisation buffer. This process was then repeated combining the Adaptor 2R-ligated Tester 1-2 with the Rsa I-digested driver cDNA and 4×hybridisation buffer. The samples were incubated in a thermal cycler at 98° C. for 1.5 min followed by incubation at 68° C. for 8 hr.  
     [0226] Second Hybridisation  
     [0227] 1 μl of Driver cDNA (i.e. the Rsa I-digested cDNA (see above)), 1 μl 4×Hybridisation buffer and 2 μl Sterile H 2 O were all combined in a fresh tube. 1 μl of this mix was then removed and placed in a new tube, overlaid with 1 drop of mineral oil and incubated at 98° C. for 1.5 min in order to denature the driver. The following procedure was used to simultaneously mix the driver with hybridisation samples 1 and 2 (prepared in the first hybridisation), thus ensuring that the two hybridisation samples were mixed together only in the presence of freshly denatured driver: A micropipettor was set at 15 μl. The pipette tip was then touched onto the mineral oil/sample interface of the tube containing hybridisation sample 2. The entire sample was drawn partway into the tip before it was removed from the tube in order to draw a small amount of air into the tip. The pipette tip was then touched onto the interface of the tube containing the freshly denatured driver (i.e. the tip contained both samples separated by a small pocket of air) before the entire mixture was transferred to the tube containing hybridisation sample 1. The reaction was then incubated at 68° C. overnight. 200 μl of dilution buffer was added to the tube, which was then heated in a thermal cycler at 68° C. for 7 min. The product of this second hybridisation was stored at −20° C.  
     [0228] PCR Amplification  
     [0229] Seven PCR reactions were set up: (1) The forward-subtracted experimental cDNA, (2) the unsubtracted tester control (see preparation of the adaptor ligated tester cDNA), (3) the reverse-subtracted experimental cDNA, (4) the unsubtracted tester control for the reverse subtraction, (5) the subtracted control skeletal muscle cDNA, (6) the unsubtracted tester control for the control subtraction, and (7) the PCR control subtracted cDNA (provided in the kit). The PCR control subtracted cDNA was required to provide a positive PCR control as it contained a successfully subtracted mixture of Hae III-digested φX174 DNA.  
     [0230] The PCR templates were prepared by aliquotting 1 μl of each diluted cDNA (i.e., each subtracted sample from the second hybridisation and the corresponding diluted unsubtracted tester control produced by the adaptor ligation see above) into an appropriately labeled tube. 1 μl of the PCR control subtracted cDNA was placed into a fresh tube. A master mix for all of the primary PCR tubes, plus one additional reaction, was then prepared by combining 19.5 μl of sterile water, 2.5 μl of 10×PCR reaction buffer, 0.5 μl of dNTP Mix (10 mM), 1.0 μl of PCR primer 1 (10 μM) and 0.5 μl of 50×Advantage cDNA Polymerase Mix. 24 μl of Master Mix was then aliquotted into each of the 7 reaction tubes prepared above and the mixture was overlaid with 50 μl of mineral oil, before being incubated in a thermal cycler at 75° C. for 5 min in order to extend the adaptors. Thermal cycling was then immediately started using the following protocol: 94° C. 25 sec (1 cycle), 94° C. 10 sec, 66° C. 30 sec and 72° C. 1.5 min (32 cycles).  
     [0231] 3 μl of each primary PCR mixture was then diluted in 27 μl of H 2 O, 1 μl of each of these dilutions was then placed into a fresh tube.  
     [0232] A master mix for the secondary PCRs, (plus an additional reaction) was set up by combining 18.5 μl of sterile water, 2.5 μl of 10×PCR reaction buffer, 1.0 μl of Nested PCR primer 1 (10 μM), 1.0 μl of Nested PCR primer 2R (10 μM), 0.5 μl of dNTP Mix (10 mM) and 0.5 μl of 50×Advantage cDNA Polymerase Mix per reaction. 24 μl of this Master Mix was then added into each reaction tube containing the 1 μl diluted primary PCR mixture. The following PCR protocol was then carried out: 94° C. 10 sec, 68° C. 30 sec and 72° C. 1.5 min (12 cycles). The reaction products were then stored at −20° C.  
     [0233] Ligation Into a Vector/Transformation &amp; PCR  
     [0234] The products of the PCR amplification (enriched for differentially expressed cDNAs) were ligated into the pCR2.1-TOPO vector using a T/A cloning kit (Invitrogen), transformed into TOPO One Shot competent cells according to the manufacturers protocol and grown up on LB (Luria-Bertani) Agar plates overnight at 37° C. 1,000 colonies were then individually picked (using fresh sterile tips) and dipped into 5 μl of sterile water which had been aliquotted previously into 96 well PCR plates. The water/colonies were heated in a thermal cycler at 100° C. for 10 minutes in order to burst the cells, thus releasing the plasmids containing a differentially expressed cDNA insert. The 5 μl of water/plasmid was then used as a template in a PCR reaction (see below) using M13 Forward and Reverse primers (10 ng/μl), complementary to the M13 site present on either side of the cloning site on the vector. 5 μl of the PCR product was then run on a 2% agarose gel and stained by ethidium bromide. PCR products of an amplified insert were identified and 5 μl of the remainder of the PCR product (i.e. from the 15 μl that had not been run on the gel) was diluted {fraction (1/10)} with water. 5 μl of the diluted PCR product was then used as a template in a sequencing reaction.  
     [0235] Sequencing  
     [0236] A sequencing reaction containing M13 primer (3.2 pmol/μl), ‘BigDye’ reaction mix (i.e. AmpliTaq® DNA polymerase, MgCl 2 , buffer and fluorescent dNTPs [each of the four deoxynucleoside triphosphates is linked to a specific fluorescent donor dye which in turn is attached to a specific acceptor dye]) and cDNA template (diluted PCR product) was set up. The reaction was carried out on a thermal cycler for 25 cycles of 10 seconds at 96° C., 20 seconds at 50° C. and 4 minutes at 60° C. Each reaction product was then purified through a hydrated Centri-Sep column, and lyophilised. The pellets were resuspended in Template Supression Reagent and sequenced on an ABI Prism 310 Genetic Analyser. The analyser uses an ion laser to excite the specific donor dye that transfers its energy to the acceptor dye, which emits a specific energy spectrum that can be read by the sequencer.  
     [0237] The differentially expressed genes of the streptozocin-induced diabetes experiment and of the CCI experiment were sequenced at Parke-Davis, Cambridge and at the applicant&#39;s core sequencing facility in Ann Arbor, Mich., USA.  
     [0238] Bioinformatics  
     [0239] The sequencing results were analysed using the computer program CHROMAS in which the vector and adaptor sequences were clipped off, leaving only the nucleotide sequence of the differentially expressed gene. Each sequence was then checked for homology to known genes, Expressed Sequence Tags (ESTs) and Proteins using various Basic Local Alignment Search Tool (BLAST) searches against the Genbank sequence database at the National Centre for Biotechnology Information, Bethesda, Md., USA (NCBI).  
     [0240] Lists were derived called STZup and STZdown and CCIup and CCIdown that contain the nucleic acid sequences from the forward and back subtracted libraries respectively. In each list there are given accession numbers and descriptions for the known rat genes identified, and where available corresponding mouse or human genes. Sequences that are considered to be of interest and that are down-regulated both in a streptozocin-induced diabetes model and in a chronic constrictive injury pain model are identified in Tables I-VI above and are listed below.  
     [0241] References have been given where available for the sequences that have been found, and sequence listings have been given in the form in which they currently appear in publicly searchable databases e.g. the NCBI databaase (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland, Md. 20894, USA, www.ncbi.nlm.nih.gov). These sequence listings are given for the purposes of identification only. The invention includes the use of subsequently revised versions of the above sequences (which may incorporate small differences to the version set out herein) and homologous sequences or similar proteins in other species as determined by a high percentage identity (e.g. above 50%, preferably above 90%), length of alignment and functional equivalence.  
     [0242] 1. Noguchi, T., Inoue, H. and Tanaka, T., The M-1- and M-2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing,  J. Biol. Chem . 261, 13807-13812 (1986).  
     [0243] 2. Takenaka, M., Noguchi, T., Inoue, H., Yamada, K., Matsuda, T. and Tanaka, T., Rat pyruvate kinase M gene: Its complete structure and characterization of the 5′-flanking region,  J. Biol. Chem . 264, 2363-2367 (1989).  
     [0244] 3. Zhou, Q. Y., Grandy, D. K., Thambi, L., Kushner, J. A., Van Tol, H. H., Cone R., Pribnow, D., Salon, J., Bunzow, J. R. and Civelli, O., Cloning and expression of human and rat D1 dopamine receptors,  Nature , 347 (6288), 76-80 (1990)  
     [0245] 4. Sullivan, R., Chateauneuf, A., Coulombe, N., Kolakowski, L. F. Jr., Johnson, M. P., Hebert, T. E., Ethier, N., Belley, M., Metters, K., Abramovitz, M., O&#39;Neill, G. P. and Ng, G. Y. K., Coexpression of full-length gamma-aminobutyric acid(B) (GABA(B)) receptors with truncated receptors and metabotropic glutamate receptor 4 supports the GABA(B) heterodimer as the functional receptor,  J. Pharmacol. Exp. Ther . 293 (2), 460-467 (2000)  
     [0246] 5. Premont, R. T., Claing, A., Vitale, N., Freeman, J. L., Pitcher, J. A., Patton, W. A., Moss, J., Vaughan, M. and Lefkowitz, R. J., beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein,  Proc. Natl. Acad. Sci. U.S.A ., 95 (24), 14082-14087 (1998).  
     [0247] 6. Wells, D. and Kedes, L., Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs,  Proc. Natl. Acad. Sci. U.S.A ., 82 (9), 2834-2838 (1985).  
     [0248] 7. Ayte, J., Gil-Gomez, G. and Hegardt, F. G., Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase,  Nucleic Acids Res ., 18 (12), 3642 (1990).  
     [0249] 8. Teruya, J. H., Kutsunai, S. Y., Spear, D. H., Edwards, P. A. and Clarke, C. F., Testis-specific transcriptional initiation sites of rat farnesyl pyrophosphate synthetase mRNA,  Mol. Cell. Biol ., 10, 2315-2326 (1990).  
     [0250] 9. Beck, K. F., Schreglmann, R., Stathopulos, I., Klein, H., Hoch, J. and Schweizer, M., The fatty acid synthase (FAS) gene and its promoter in  Rattus norvegicus, DNA Seq ., 2 (6), 359-386 (1992).  
     [0251] 10. Mill, J. F., Mearow, K. M., Purohit, H. J., Haleem-Smith, H., King, R. and Freese, E., Cloning and functional characterization of the rat glutamine synthetase gene,  Brain Res. Mol. Brain Res ., 9 (3), 197-207 (1991).  
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     [0253] 12. Giallongo, A., Feo, S., Moore, R., Croce, C. M. and Showe, L. C., Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase,  Proc. Natl. Acad. Sci. U.S.A ., 83 (18), 6741-6745 (1986).  
     [0254] 13. Carper, D., Nishimura, C., Shinohara, T., Dietzchold, B., Wistow, G., Craft, C., Kador, P. and Kinoshita, J. H., Aldose reductase and p-crystallin belong to the same protein superfamily as aldehyde reductase,  FEBS Lett . 220 (1), 209-213 (1987).  
     [0255] 14. Ku, C. Y., Lu, Q., Ussuf, K. K., Weinstock, G. M. and Sanborn, B. M., Hormonal regulation of cytochrome oxidase subunit messenger RNAs in rat Sertoli cells,  Mol. Endocrinol . 5 (11), 1669-1676 (1991).  
     [0256] 15. Tsuji, S., Qureshi, M. A., Hou, E. W., Fitch, W. M. and Li, S. S. -L., Evolutionary relationships of lactate dehydrogenases from mammals, birds, an amphibian, fish, barley, and bacteria: LDH cDNA sequences from Xenopus, pig and rat,  Proc. Natl. Acad. Sci. U.S.A . 91, 9392-9396 (1994).  
     [0257] 16. Taanman, J. W., Schrage, C., Ponne, N., Bolhuis, P., de Vries, H. and Agsteribbe, E., Nucleotide sequence of cDNA encoding subunit VIb of human cytochrome c oxidase,  Nucleic acids research . 17 (4), 1766 (1989).  
     [0258] 17. Triepels, R., van den Heuvel, L., Loeffen, J., Smeets, R., Trijbels, F. and Smeitink, J., The nuclear-encoded human NADH: ubiquinone oxidoreductase NDUFA8 subunit: cDNA cloning, chromosomal localization, tissue distribution, and mutation detection in complex-I-deficient patients,  Hum. Genet . 103 (5), 557-563 (1998).  
     [0259] 18. Hosokawa, Y., Suzuki, H., Toda, H., Nishikimi, M. and Ozawa, T. Complementary DNA encoding core protein II of human mitochondrial cytochrome bc-1 complex: Substantial diversity in deduced primary structure from its yeast counterpart,  J. Biol. Chem . 264, 13483-13488 (1989).  
     [0260] 19. Harnik-Ort, V., Prakash, K., Marcantonio, E., Colman, D. R., Rosenfeld, M. G., Adesnik, M., Sabatini, D. D. and Kreibich, G. Isolation and characterization of cDNA clones for rat ribophorin I: complete coding sequence and in vitro synthesis and insertion of the encoded product into endoplasmic reticulum membranes,  J. Cell Biol . 104(4), 855-863 (1987).  
     [0261] 20. Falany, C. N., Xie, X., Wang, J., Ferrer, J. and Falany, J. L., Molecular cloning and expression of novel sulphotransferase-like cDNAs from human and rat brain,  Biochem. J . 346 Pt 3, 857-864 (2000).  
     [0262] 21. Pan, W., Ko, Y. H. and Pedersen, P. L., Delta subunit of rat liver mitochondrial ATP synthase: molecular description and novel insights into the nature of its association with the F1-moiety,  Biochemistry  37 (19), 6911-6923 (1998).  
     [0263] 22. Hamajima, N., Matsuda, K., Sakata, S., Tamaki, N., Sasaki, M. and Nonaka, M., A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution,  Gene  180 (1-2), 157-163 (1996).  
     [0264] 23. McGuire, J. A., Poellinger, L., Wikstrom, A. C. and Gustafsson, J. A., Cloning and regulation by glucocorticoid receptor ligands of a rat hsp90  J. Steroid Biochem. Mol. Biol . 42 (8), 813-822 (1992).  
     [0265] 24. Roach, A., Boylan, K. B., Horvath, S., Prusiner, S. B. and Hood, L. E., Characterization of cloned cdna representing rat myelin basic protein: absence of expression in brain of shiverer mutant mice,  Cell  34, 799-806 (1983)  
     [0266] 25. Hoshino, A., Hisayasu, S. and Shimada, T., Complete sequence analysis of rat transferrin and expression of transferrin but not lactoferrin in the digestive glands,  Comp. Biochem. Physiol. B  113, 491-497 (1996).  
     [0267] 26. Chin, S. S. and Liem, R. K., Expression of rat neurofilament proteins NF-L and NF-M in transfected non-neuronal cells, Eur. J. Cell Biol. 50 (2), 475-490 (1989).  
     [0268] 27. Lai, C., Brow, M. A. D., Nave, K. -A., Noronha, A. B., Quarles, R. H., Bloom, F. E., Milner, R. J. and Sutcliffe, J. G., Two forms of 1B236/myelin-associated glycoprotein, a cell adhesion molecule for postnatal neural development, are produced by alternative splicing,  Proc. Natl. Acad. Sci. U.S.A . 84, 4337-4341 (1987).  
     [0269] 28. Napolitano, E. W., Chin, S. S. M., Colman, D. R. and Liem, R. K. H., Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein,  J. Neurosci . 7, 2590-2599 (1987).  
     [0270] 29. Nakayama, M., Miyake, T., Gahara, Y., Ohara, O. and Kitamura, T., A novel RING-H2 motif protein downregulated by axotomy: its characteristic localization at the postsynaptic density of axosomatic synapse,  J. Neurosci . 15 (7 Pt 2), 5238-5248 (1995).  
     [0271] 30. Kuwano, R., Usui, H., Maeda, T., Fukui, T., Yamanari, N., Ohtsuka, E., Ikehara, M. and Takahashi, Y., Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain,  Nucleic Acids Res . 12 (19), 7455-7465 (1984).  
     [0272] 31. Zauner, W., Kratz, J., Staunton, J., Feick, P. and Wiche, G., Identification of two distinct microtubule binding domains on recombinant rat MAP 1B,  Eur. J. Cell Biol . 57 (1), 66-74 (1992).  
     [0273] 32. Ozaki, T., Irie, K. and Sakiyama, S., Molecular cloning and cell cycle-dependent expression of a novel gene that is homologous to cdc37 , DNA Cell Biol . 14 (12), 1017-1023 (1995).  
     [0274] 33. Albertsen, H. M., Smith, S. A., Mazoyer, S. S., Fujimoto, E., Stevens, J., Williams, B., Rodriguez, P., Cropp, C. S., Slijepcevic, P. and Carlson, M., A physical map and candidate genes in the BRCA1 region on chromosome 17q12-21 , Nature Genet . 7 (4), 472-479 (1994).  
     [0275] 34. Dieffenbach, C. W., SenGupta, D. N., Krause, D., Sawzak, D. and Silverman, R. H., Cloning of murine gelsolin and its regulation during differentiation of embryonal carcinoma cells,  J. Biol. Chem . 264, 13281-13288 (1989).  
     [0276] 35. Geisert, E. E. Jr., Murphy, T. P., Irwin, M. H. and Larjava, H., A novel cell adhesion molecule, G-CAM, found on cultured rat glia ,  Neurosci. Lett . 133 (2), 262-266 (1991); Irwin, .H. and Geisert, .E. Jr., The upregulation of a glial cell surface antigen at the astrocytic scar in the rat,  Neurosci. Lett . 154 (1-2), 57-60 (1993); Geisert, E. E. Jr., Yang, L. and Irwin, M. H., Astrocyte growth, reactivity, and the target of the antiproliferative antibody, TAPA,  J. Neurosci . 16 (17), 5478-5487 (1996).  
     [0277] 36. Holz, A., Schaeren-Wiemers, N., Schaefer, C., Pott, U., Colello, R. J. and Schwab, M. E., Molecular and developmental characterization of novel cDNAs of the myelin-associated/oligodendrocytic basic protein,  J. Neurosci . 16 (2), 467-477 (1996).  
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     [0279] 38. Lai, C. H., Chou, C. Y., Ch&#39;ang, L. Y., Liu, C. S. and Lin, Wc., Identification of novel human genes evolutionarily conserved in caenorhabditis elegans by comparative proteomics,  Genome Res . 10 (5), 703-713 (2000).  
     [0280] 39. Nagase, T., Seki, N., Tanaka, A., Ishikawa, K. and Nomura, N., Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1 , DNA Res . 2 (4), 167-174 (1995).  
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     [0287] 46. Lemke, G. and Axel, R., Isolation and sequence of a cDNA encoding the major structural protein of peripheral myelin,  Cell  40, 501-508 (1985).  
    
     
       
         1 
         
           
             117  
           
           
             1  
             531  
             PRT  
             Rattus norvegicus  
             
               Pyruvate kinase M1 subunit  
             
           
            1 

Met Pro Lys Pro Asp Ser Glu Ala Gly Thr Ala Phe Ile Gln Thr Gln 
1               5                   10                  15 

Gln Leu His Ala Ala Met Ala Asp Thr Phe Leu Glu His Met Cys Arg 
            20                  25                  30 

Leu Asp Ile Asp Ser Ala Pro Ile Thr Ala Arg Asn Thr Gly Ile Ile 
        35                  40                  45 

Cys Thr Ile Gly Pro Ala Ser Arg Ser Val Glu Met Leu Lys Glu Met 
    50                  55                  60 

Ile Lys Ser Gly Met Asn Val Ala Arg Leu Asn Phe Ser His Gly Thr 
65                  70                  75                  80 

His Glu Tyr His Ala Glu Thr Ile Lys Asn Val Arg Ala Ala Thr Glu 
                85                  90                  95 

Ser Phe Ala Ser Asp Pro Ile Leu Tyr Arg Pro Val Ala Val Ala Leu 
            100                 105                 110 

Asp Thr Lys Gly Pro Glu Ile Arg Thr Gly Leu Ile Lys Gly Ser Gly 
        115                 120                 125 

Thr Ala Glu Val Glu Leu Lys Lys Gly Ala Thr Leu Lys Ile Thr Leu 
    130                 135                 140 

Asp Asn Ala Tyr Met Glu Lys Cys Asp Glu Asn Ile Leu Trp Leu Asp 
145                 150                 155                 160 

Tyr Lys Asn Ile Cys Lys Val Val Glu Val Gly Ser Lys Ile Tyr Val 
                165                 170                 175 

Asp Asp Gly Leu Ile Ser Leu Gln Val Lys Glu Lys Gly Ala Asp Tyr 
            180                 185                 190 

Leu Val Thr Glu Val Glu Asn Gly Gly Ser Leu Gly Ser Lys Lys Gly 
        195                 200                 205 

Val Asn Leu Pro Gly Ala Ala Val Asp Leu Pro Ala Val Ser Glu Lys 
    210                 215                 220 

Asp Ile Gln Asp Leu Lys Phe Gly Val Glu Gln Asp Val Asp Met Val 
225                 230                 235                 240 

Phe Ala Ser Phe Ile Arg Lys Ala Ala Asp Val His Glu Val Arg Lys 
                245                 250                 255 

Val Leu Gly Glu Lys Gly Lys Asn Ile Lys Ile Ile Ser Lys Ile Glu 
            260                 265                 270 

Asn His Glu Gly Val Arg Arg Phe Asp Glu Ile Leu Glu Ala Ser Asp 
        275                 280                 285 

Gly Ile Met Val Ala Arg Gly Asp Leu Gly Ile Glu Ile Pro Ala Glu 
    290                 295                 300 

Lys Val Phe Leu Ala Gln Lys Met Met Ile Gly Arg Cys Asn Arg Ala 
305                 310                 315                 320 

Gly Lys Pro Val Ile Cys Ala Thr Gln Met Leu Glu Ser Met Ile Lys 
                325                 330                 335 

Lys Pro Arg Pro Thr Arg Ala Glu Gly Ser Asp Val Ala Asn Ala Val 
            340                 345                 350 

Leu Asp Gly Ala Asp Cys Ile Met Leu Ser Gly Glu Thr Ala Lys Gly 
        355                 360                 365 

Asp Tyr Pro Leu Glu Ala Val Arg Met Gln His Leu Ile Ala Arg Glu 
    370                 375                 380 

Ala Glu Ala Ala Val Phe His Arg Leu Leu Phe Glu Glu Leu Ala Arg 
385                 390                 395                 400 

Ala Ser Ser Gln Ser Thr Asp Pro Leu Glu Ala Met Ala Met Gly Ser 
                405                 410                 415 

Val Glu Ala Ser Tyr Lys Cys Leu Ala Ala Ala Leu Ile Val Leu Thr 
            420                 425                 430 

Glu Ser Gly Arg Ser Ala His Gln Val Ala Arg Tyr Arg Pro Arg Ala 
        435                 440                 445 

Pro Ile Ile Ala Val Thr Arg Asn Pro Gln Thr Ala Arg Gln Ala His 
    450                 455                 460 

Leu Tyr Arg Gly Ile Phe Pro Val Leu Cys Lys Asp Ala Val Leu Asp 
465                 470                 475                 480 

Ala Trp Ala Glu Asp Val Asp Leu Arg Val Asn Leu Ala Met Asn Val 
                485                 490                 495 

Gly Lys Ala Arg Gly Phe Phe Lys Lys Gly Asp Val Val Ile Val Leu 
            500                 505                 510 

Thr Gly Trp Arg Pro Gly Ser Gly Phe Thr Asn Thr Met Arg Val Val 
        515                 520                 525 

Pro Val Pro 
    530 

 
           
             2  
             531  
             PRT  
             Rattus norvegicus  
             
               Pyruvate kinase M2 subunit  
             
           
            2 

Met Pro Lys Pro Asp Ser Glu Ala Gly Thr Ala Phe Ile Gln Thr Gln 
1               5                   10                  15 

Gln Leu His Ala Ala Met Ala Asp Thr Phe Leu Glu His Met Cys Arg 
            20                  25                  30 

Leu Asp Ile Asp Ser Ala Pro Ile Thr Ala Arg Asn Thr Gly Ile Ile 
        35                  40                  45 

Cys Thr Ile Gly Pro Ala Ser Arg Ser Val Glu Met Leu Lys Glu Met 
    50                  55                  60 

Ile Lys Ser Gly Met Asn Val Ala Arg Leu Asn Phe Ser His Gly Thr 
65                  70                  75                  80 

His Glu Tyr His Ala Glu Thr Ile Lys Asn Val Arg Ala Ala Thr Glu 
                85                  90                  95 

Ser Phe Ala Ser Asp Pro Ile Leu Tyr Arg Pro Val Ala Val Ala Leu 
            100                 105                 110 

Asp Thr Lys Gly Pro Glu Ile Arg Thr Gly Leu Ile Lys Gly Ser Gly 
        115                 120                 125 

Thr Ala Glu Val Glu Leu Lys Lys Gly Ala Thr Leu Lys Ile Thr Leu 
    130                 135                 140 

Asp Asn Ala Tyr Met Glu Lys Cys Asp Glu Asn Ile Leu Trp Leu Asp 
145                 150                 155                 160 

Tyr Lys Asn Ile Cys Lys Val Val Glu Val Gly Ser Lys Ile Tyr Val 
                165                 170                 175 

Asp Asp Gly Leu Ile Ser Leu Gln Val Lys Glu Lys Gly Ala Asp Tyr 
            180                 185                 190 

Leu Val Thr Glu Val Glu Asn Gly Gly Ser Leu Gly Ser Lys Lys Gly 
        195                 200                 205 

Val Asn Leu Pro Gly Ala Ala Val Asp Leu Pro Ala Val Ser Glu Lys 
    210                 215                 220 

Asp Ile Gln Asp Leu Lys Phe Gly Val Glu Gln Asp Val Asp Met Val 
225                 230                 235                 240 

Phe Ala Ser Phe Ile Arg Lys Ala Ala Asp Val His Glu Val Arg Lys 
                245                 250                 255 

Val Leu Gly Glu Lys Gly Lys Asn Ile Lys Ile Ile Ser Lys Ile Glu 
            260                 265                 270 

Asn His Glu Gly Val Arg Arg Phe Asp Glu Ile Leu Glu Ala Ser Asp 
        275                 280                 285 

Gly Ile Met Val Ala Arg Gly Asp Leu Gly Ile Glu Ile Pro Ala Glu 
    290                 295                 300 

Lys Val Phe Leu Ala Gln Lys Met Met Ile Gly Arg Cys Asn Arg Ala 
305                 310                 315                 320 

Gly Lys Pro Val Ile Cys Ala Thr Gln Met Leu Glu Ser Met Ile Lys 
                325                 330                 335 

Lys Pro Arg Pro Thr Arg Ala Glu Gly Ser Asp Val Ala Asn Ala Val 
            340                 345                 350 

Leu Asp Gly Ala Asp Cys Ile Met Leu Ser Gly Glu Thr Ala Lys Gly 
        355                 360                 365 

Asp Tyr Pro Leu Glu Ala Val Arg Met Gln His Leu Ile Ala Arg Glu 
    370                 375                 380 

Ala Glu Ala Ala Ile Tyr His Leu Gln Leu Phe Glu Glu Leu Arg Arg 
385                 390                 395                 400 

Leu Ala Pro Ile Thr Ser Asp Pro Thr Glu Ala Ala Ala Val Gly Ala 
                405                 410                 415 

Val Glu Ala Ser Phe Lys Cys Cys Ser Gly Ala Ile Ile Val Leu Thr 
            420                 425                 430 

Lys Ser Gly Arg Ser Ala His Gln Val Ala Arg Tyr Arg Pro Arg Ala 
        435                 440                 445 

Pro Ile Ile Ala Val Thr Arg Asn Pro Gln Thr Ala Arg Gln Ala His 
    450                 455                 460 

Leu Tyr Arg Gly Ile Phe Pro Val Leu Cys Lys Asp Ala Val Leu Asp 
465                 470                 475                 480 

Ala Trp Ala Glu Asp Val Asp Leu Arg Val Asn Leu Ala Met Asn Val 
                485                 490                 495 

Gly Lys Ala Arg Gly Phe Phe Lys Lys Gly Asp Val Val Ile Val Leu 
            500                 505                 510 

Thr Gly Trp Arg Pro Gly Ser Gly Phe Thr Asn Thr Met Arg Val Val 
        515                 520                 525 

Pro Val Pro 
    530 

 
           
             3  
             11788  
             DNA  
             Rattus norvegicus  
             
               Pyruvate kinase  
             
           
            3 

cccgggcgag cgccgggagg gtggagagtc accgggcggg gctggaggaa tgtccgggac     60 

ctataaatct gggcaacgcc ctggtaggcc agggcagatg gggcacctgg gcagaattcc    120 

aaaatgggat tatgtagcct ctgaggtcct aaagcaacag gtggcggacc acccggggat    180 

ctaggggtgg tggcggcggt ggacccgagg gcgggtcctg cctcctcacc acttccccat    240 

tggccatcag aatgacccat gcgcaatttt ggtttgcaat gtccttccgc cacggaaggt    300 

agtccccctc aaaagggcaa cctgcttgtc ccgcctaccc tgcgactctc tcagaaggtg    360 

cgggtgcctg ttgagaggcg gggctctgct agctgctgcc cggattgggc gaggggcggg    420 

gctgcggagg gattgcggcg gcccgcagca gtgataacct tgaggcccag tctgcgcagc    480 

cccgcacagc agcgacccgt cctaagtcga cagacgtcct ctttaggtat tgcaacagga    540 

tctgaagtac gcccgaggtg agcggggaga acctttgcca ttctgtggcc cagagccaat    600 

gccatagtgg atcactagtg ggtggctgca atgtcaggtg gcagagccag gcttgggtgc    660 

atgttcttta ggtatccaga aaagccgatg tccagggtag agtgcgggtc tttgggaagg    720 

cagggaagag caaggagatg tggagtgcag gctgtgtgct caggagtttg aaaggagcct    780 

cgtggcctga aaattgattc aagtctctgg tattctgtga ggtacatccc aggttctcgc    840 

ttactcagcc aactatccag ctcagtgagt gcttagctgt aagtcgtaat aaagtaaaag    900 

caaaggtcag gacagctgtt gggattgtga cctgggtgat taattgcctg gaacagatgt    960 

tcagactact gtgtcgtggt ccctatcaga cagagaagga gactctagat agccagaact   1020 

ggggctggga aagctgggtc taatgtcagc tcttgggaag agtagtgact ttcaaggtca   1080 

acctcggttg cacagtggat ttcaggccag catagactag ctactgtgtg tgattccatc   1140 

tcctaggtta ctgaagactc cttggtgagg ttgtaatgac tttggagcca tttcaatgtc   1200 

cttgtgcaaa atttgctttg tgtgtcttgt acatggtgct gcttttttgc ttcttgtcca   1260 

gaaaaaaact tgttgcctgt aatttggacc ttattcctgt gtcggataga ggctaaggaa   1320 

tacatttagg tgatacctca aacctaggac tttaaaaaga aaggacttgg gacagggttc   1380 

gtctcttggc agcaggatcg tttaggtcat ggcgaacatg ataaagaggc tatgtcagtg   1440 

ccaaagcagg tttgacattg tcgtgatagg cttcccagat gttcgtgtgc tgacacatta   1500 

gctacgtgct ctgcaggagt ggggaggttt ccttgtgtgg gaaggtatgt tctctctagg   1560 

gttggtgtga agctcttcaa tagatggcag ccgttgtaag aagctctgct taaactggca   1620 

gccagggtgt gtatgtgcac tgggaaggaa gctacttttg ttcaccaagg tctttgctgt   1680 

tcgttcatag tcacaggagc cctttgccca atttgagtag tacccacata accagcacgt   1740 

gccaggcctc tgagtgactt gtgcacaaag cagtcttatt gggttaatgt gtaagcggga   1800 

gacagtctcc tatgccaaat ggttcattaa gcactgccta gtctaattga actcttcaaa   1860 

tctccgggat ccaggatctc agaaaccatg cccaagccag acagcgaagc agggactgcc   1920 

ttcattcaga cccagcagct ccatgcagcc atggctgaca ccttcctgga acacatgtgc   1980 

cgcctggaca ttgactccgc acccatcacg gcccgcaaca ctgggatcat ctgtaccatt   2040 

ggtgagtgag tgtggccccc ttcgcagggc ttctgcctgg tttgaaaggc gtaataacca   2100 

ttgcagggct aacctaggtt ctgagagaca tgtccacact tttagaggaa cgtattggtg   2160 

tacttcttct gcacttggtg aggttcaggg tggtctagtg gcctttgcag gagtagttca   2220 

gggacttcag atctttttcc tgtgctgtca ctccactttc tgcagaattt atagaaaatg   2280 

atagtgtcct tccttacata taaattaaga gagtccttcc ttacatataa atcaagagag   2340 

ttgactagca cagtagtctt tctggacttt ggaaagacct ttctctaaat ttgtggaggt   2400 

attaaaaaca aaggacaagg agttgccttt gatagtagca gatttcatat actttcctac   2460 

ctgagaatca tgattttctt cctgtggctc taaatgtttc ttggtaggcc ctgcttcccg   2520 

atctgtggag atgctgaagg agatgattaa gtctgggatg aatgtggctc ggctgaattt   2580 

ctctcatgga acccatgagg tgagtggcag cttgatccag gaggttcggg acttgctgct   2640 

gctgtgctcc aggcttctca gtgaatggac gttgctctac ttacatttag atcccctctc   2700 

tccctcttcc cctctggcca acttcattgg catcaacaac ctgcctgctt tgtcgccggc   2760 

caggttatcc ctaccatcaa gcttcaccag ggctttctta ttctatgtgc accttcgatg   2820 

aggggctgtg gctgtctgtc ttggatgagg ggctgtggct gtctgtcttg gttttaacca   2880 

ttcctggaga cggtggaatg aatcgtagct ccctcgagct cttggcctaa ttgatcatct   2940 

ttggctccag ggtggctcag ggcatgggaa ctggacttgg gtgtggagaa cacctacctg   3000 

tcaatctccc cttctttctc tccagtacca tgcagagact atcaagaatg tccgtgcagc   3060 

cacagaaagc tttgcatctg atcccattct ctaccgacct gttgcggtgg ctctggatac   3120 

aaagggacct gagatccgga ctggactcat caagggcgtg agtatccagg agtttaggtc   3180 

tttaaatgag aatatttttc atctgcctgg taggaattat tatagatgca catttttggt   3240 

atgtgaataa catacttaag tctcactctg gggacctggt ttgttgtttg ttttgtttgt   3300 

ttccctcaat aaacaaattc aggatttaca gaaaggtgat cggtttcttg gggctttgag   3360 

ccagagtttg agcgccgcca tcagggtgtt ggcgtccaca gtcacacgcc tctgctgtct   3420 

ttaatctaga gcggcaccgc agaggtggag ctgaagaagg gagccacact gaagatcacc   3480 

ctggacaacg cctacatgga gaagtgcgac gagaacatcc tgtggctgga ctataagaac   3540 

atctgcaagg tggtggaggt gggcagcaag atctacgtgg acgatgggct catctccctg   3600 

caggtgaagg agaaaggtat gtgtggtgta cagtccacgg cccaatgcca ctcccatccc   3660 

cagaactctg gtaagcactt aacctagcat gtatgaattg gtctcccaag gtcaaaagtt   3720 

taaggtggtt gttggtctgc atccctggcc tgtctgaaac actgcctgag aaaaaataga   3780 

caaataacct acaaaggcct atgtgtacac ctctaccctt tagttccagc actcgggaat   3840 

cagcaggtgt gtgagttctc atgtgtaagg actactcctg tatgcctaga atgagtctag   3900 

agttctcttg gcttctcaca aactgagata gatggtcttg atccctttca cacaggtgct   3960 

gactacctgg tgacagaagt ggaaaatggt ggctccttgg gcagcaagaa gggcgtgaac   4020 

ctgcctggtg ctgctgtgga cctccctgct gtgtcagaaa aggacatcca ggacctgaag   4080 

tttggggtgg agcaggacgt ggacatggtg tttgcgtctt tcatccgcaa ggcggctgac   4140 

gtgcatgagg ttaggaaggt cctgggagag aagggcaaga acatcaagat catcagcaaa   4200 

atcgagaacc atgaaggtgt ccgcaggtga aggtccactc ctaccgtgtg cctggggtgt   4260 

ggccttgagg gcacctctgt cagggcccag gaaagctctg tccattcttt ggttgtactt   4320 

cctgttctat agcatctttc tttttatcag gtttgatgag attttggagg ccagcgatgg   4380 

aatcatggta gctcgtggtg acctgggcat tgagattccg gcagagaagg tcttcctagc   4440 

tcagaagatg atgattggac gatgcaaccg agctgggaag ccagtcatct gcgccaccca   4500 

ggcatgtgct atcccttcct tctgtgttct ccacctagga gacctggtct tgacctggcc   4560 

tttaggtaca cgtacccgca catagctatg acctgcgtac ctgtgcaagc ttcggggaat   4620 

tgccctggaa tcatcactga agatgtcctg ctcttccatt atttagtgac tttcatttag   4680 

cggtggtctc ttacttaata aaaacccttg tttgtcccct cctagatgct ggagagcatg   4740 

atcaagaagc cacgccccac ccgtgctgaa ggcagtgacg tggccaatgc agtcctagat   4800 

ggagctgact gcatcatgct gtccggagaa acagccaaag gggactaccc tctggaggct   4860 

gttcgcatgc agcacctggt gagtaagtcc tcagagtcct ggggtagaag agctctgctg   4920 

gagaggcctc tgtccagtct tgttacattg ctcccgtcac agcaaggaga gtgaggtttg   4980 

tggagtgtgc ttgagtttca ttgtgctttc actgcctgca cctgcccctt tgtcctgctc   5040 

tggggattac ataggccaca tctggctaaa atatcaaggt cctaggatgc agtcaaggga   5100 

tgccttcctt gtggacaccc agagggcctg ggtacctcta ttctaaagga gccaagagtt   5160 

tgttcagcta ttcgccttgt tacctctcat ttggtctcct gtggtctgcc catgggcaat   5220 

gcttctctcc actgcagctg tagccatact gagctgcttt aagctggccg tgcatggtgc   5280 

ctgtgacatg ggacttcctc ccttgctgtg ccagacccaa ctcggggcta caaatagctc   5340 

tgggggtggg gaatgggtgc taatttagca ggttctgtct ggtctaggaa ttataaagac   5400 

ttctcaggca tattatgtgc tcttttgatt aagtcttctg gtttcagtaa gatagggtct   5460 

ggcgcaagct tgtaattcca acaatcaaat caagcctgcg gcaggaagat ggcttgagat   5520 

taggctggac tagaatgaga catcttgtag aagcaacagg cgtcagtgac ctgtccctgc   5580 

ggactttcca cccaggcctc cgtcttctgt gttctgctcc agaacttctt ccggagcaga   5640 

acaattatct ggggcatcag ttagggaccg ttgcctgata aaggcatggt ggattagctt   5700 

tttgggggtt ttctattccc ttttcagcca acaaagaacc acagtatctt ttgtggctcc   5760 

agtttcccac aagccccccc aaacttaaga caagtgaaag aaaagaagga agccccttct   5820 

gggttaggca tcttaccttg ctttatatgt atatgagagt tgagtagatt ccatgatctt   5880 

tccatggatt tatctgaatt atagctgtag ccagatgttt gcctatattg aaacagacca   5940 

gcgctttgac tcggctggtc atgacctgtt ctgattggaa ttgtgactgg tcctcatagc   6000 

ttctcatagc tgcaggcatt ctctcccaga agacttttcc cttccttcaa tctcccccac   6060 

agtagctgcc ttcctggttt acaccaaaac cccttttcta cttacctggc ctcacagtgg   6120 

tcaggaacag gactttgacc aggtattcta aagcatgtag tcacataaat gtatttttgg   6180 

atagctcaag aagaccttgc ccaaagtggc ggttgatgag attaattaca attaattata   6240 

tgtgtatgag ttagttttgg tgtcctgtcc ttttctcatg ccctggtctt tcctatgaac   6300 

agacacctta gaacctcgag gctgggattg catggccctg ctcagaagat gagtcacaga   6360 

gtccgggtta gactgtggct accccctcag ggatacaaat ttctctatca gttaacactt   6420 

aggacagctt ctcccctttc tttatctgtt tgctgtttcc tcctgtgtct aactgattca   6480 

gttcaaaacc tgtaattaaa agctagacat cccagctgtg gttgcttcct gtccatgcct   6540 

cttgtccttt gggcttgcct gcattccatg cttaaccaaa agattacttt ccacttgcaa   6600 

atctgctaat gctaccaata aaatcgagtg ctggtttcat atcattcctg gaattgcact   6660 

ctctaaaact ttatttcttt atttctaatg cttggtttta cttgaacagg tggtctgtat   6720 

ataacacatt tgctatcctg taaccgtttt aattattgca gtttgaatct gtgtcttgaa   6780 

aggcctgtgt gctttcccag gcgtctgcct cctcacatgg ctgttcagtg taccgtgttc   6840 

aagtgagcca gtcgaccact gttctgttta gaacttgtgc actgcaactt tggctctttt   6900 

gaccctcacc cccagctttc agagctgccc gagtgtttcc actgtaagcc aagtgcaagc   6960 

gctcactctt gtgtgtaggc ggagttggat gccttgtaac cacatagcca tgtgaggagg   7020 

ggacgccttt ttcttcctgt aagctgtgtc aggaggcagt gtggtcaagc ggaagtgtag   7080 

ttggctccac cttggcatct ttccatgcca gggtcccttt ctcagcttta atcaaaaaca   7140 

aaagaaatga tggatggtgc atgcctagca cttgggaggc tgaggcaaag aaaatagtgg   7200 

tgttttgagg ctgaccatgg caagttcaag gccagtaggt gctatggtaa gatcctatct   7260 

caaacctgtg tggggttgag aattggcttt ttttgtttgt ttgtttttag aaacaggaaa   7320 

tcccaaaagg atactatctt tccagaaggg aaggaaggat ctgtccgtgt gtttgaacag   7380 

aaaagagagc cacccaaaat cccacatata ataccattcc aggctttgac catcctgcct   7440 

ctgtatctgc atggagaaga aaagattaac ctaagagttc cttcctctca ttagttccct   7500 

gtctttccat gtgttgtctc ttgtttttgc cttcatcctt cttccttatc cttcctaccc   7560 

taaaccttac agatagctcg agaggctgag gcagccgtgt tccaccgcct gctgtttgaa   7620 

gagcttgcgc gagcctccag tcaatccaca gaccccctgg aggccatggc catgggcagc   7680 

gtggaggcct cttataaatg tttagcagca gctttgatag ttctgacgga gtccggcagg   7740 

tagggccctg agggcaggta tcattataag ataaccagct tctcacacaa ctagggccca   7800 

ctgtgtgcct gagcctgggc atagcctctc tcctgcagga aggcagccaa ggaggtcacg   7860 

atagggcagg accaaaggat tccctagtgg gtacagtgga agtcacaggc actggttcag   7920 

gatggttccc tgtggagttt ctaatcttgc tcaagtttca gaaacgtgtt agtgaactca   7980 

tctttctcct ggctttttgt gcccaggaca tgttccttcc cagttgcctg tgactcttct   8040 

ccttcatttg tgacaaagct ctgacaaagc cctgtccccc ttcctcgtcc ctctggacgg   8100 

atgttgctcc cctagattgc ccgagaggca gaggctgcca tctaccactt gcagttattc   8160 

gaggaactcc gccgcctggc gcccattacc agcgacccca cagaagctgc cgccgtgggt   8220 

gccgtggagg cctccttcaa gtgctgcagt ggggccatta tcgtgctcac caagtctggc   8280 

aggtaggagg cggcagtggc tccctgggga tcccagagca actctgggct gattttaaga   8340 

cccctggctg ccatcaaagg actccaggaa gcactcccag gtacatcaga ttaggtggct   8400 

ccagtgccag tcagtgcagg cctgcctcag ggcctgaagc atatatacag ttttgcttag   8460 

ttacggtgtg gaagctgggc atgggtacat gcctttaatc ctcccagcat tcgggcagaa   8520 

gaggtgggca aatgtctgca ttcgtggaca gtgctggtct acaaagcaag ttccaggaca   8580 

gagccctagc tcacaaaaat taactcagct ggtaaatact acttgctaca ccaagttaga   8640 

cggatggagg agggagaaag gtaacttagc aagcgtgccc aaggacatat acagtaacac   8700 

tttttctcat cccacttgga gggagctcaa tggataaagt gttggcaatc tggatttgga   8760 

tctggcacct gtgggttttt gttgttgttt gggtttttgt tttttttttt ttttttaagt   8820 

cacctggaga agtacgttag gcctttagga gcatggggtt ttggttttta agatttattt   8880 

attatatatg aactacactc tccctatctt cagtcgcacc agaagagggt gattacagat   8940 

gattgtgtaa gccaccatgt ggttgctggg agttgaactc aggacctctg gaagagcagt   9000 

tagtgctctt aaccactgag ccatctctcc agccagcact gttctttcag aagcccaaca   9060 

catacatgac agctcacact atctaactgc agtcctgggg ggaatctaat gccctcttct   9120 

aacttccagc cgtacatgtt tatatgatgc atgtggtcag gatacccaga cagctggagg   9180 

gaaaggaagg tgcccaacac acctgtgtgc ctgtaatccc agcactcatg gtacagaggt   9240 

agaaagatca caagataggt ctaatctgag caaattctgc aacagcctag atacagaaag   9300 

ccaaacacat aaaaatcagt gtgggctgag gatgttgacc tttgtgtgaa ttggccatga   9360 

acattgtatg cagctggaag caagggatgg ggtgctgaaa aatggggacc ttaaaactaa   9420 

ttttctgaag tcctgaagtt tcgagagctc caagatacaa gttctatgct aatcctggac   9480 

tttctctgaa gagttcctgg ttccttcaaa gcccatgtag gccagctaga agcagggtat   9540 

tttctgcctg tggaatggcc acatccccta ataccttgtg tgtcctcaag atgcctagtt   9600 

aattcagtaa gcctgaaaca gatctgatag aagtaaacat atcatttgct atcggttgag   9660 

gagagagggg ctccctacat tcccaaagga tcttgattgg ccagatatta accatgcctg   9720 

gtcacaccta gattttccaa aaaaccatag aaattcagag gtttctttta gcaccaagtt   9780 

cagctgataa gactcccaaa ggagcagtat agtactctct ggcaaatact gtccactcca   9840 

gggtctctgc agataccaga agtcaggagc taggcacatg gtgcttggat tgtaaaagtt   9900 

gctggcagct acaggatggt tctggtgaga ttaggccaga gctgcctcac tgcctgatgg   9960 

aagggttcac agtgtgggag ggatcctgcc agccgtggtc ctatgggact gcccacactg  10020 

agatcaggac aatgagttaa aaaggacagg acaggtctga gggggtggcc aggcactaaa  10080 

cagtaattgt aagtgggcaa acctgtggcc tagaggtgaa ttagagggtg ctcctttggc  10140 

tgactgaaag ggtctcgtga cacaatggcc attcttccca ccttctcagg agtgctcacc  10200 

aagtggcccg gtaccgccca agggctccta tcattgctgt gacacgcaat ccccagacag  10260 

cccgccaggc ccatctgtac cgtggcatct tccctgtgct gtgtaaggat gccgtactgg  10320 

atgcctgggc tgaggacgtt gatcttcgtg tgaacttggc catgaatgtt ggtatgtagc  10380 

tggaagcaag ggatggggtg ctgggaaatg gggagcctaa aactaccatt tcctgaagcc  10440 

ctcacccaat tttgggccca aggcaaaatt aattgcctca ttaggctctt gtaagattaa  10500 

ttggagctac tgccctgtgg ggtggggcac acaagctgtt gtggtctttg ttcctatata  10560 

aggttggata tcaagagaca aggaagcagc tgaccctgaa cttgggcaag gctggccact  10620 

ctagagtcta aactgaatgg tgtccaaagg tccctgcagt ctgagttttg cctcagccct  10680 

tgtttaagct aggagacttg caccaccttg tggtaaaaag aagtaactgg caacctgctc  10740 

tcctacctga attagaagca atagggcatt tgttatcctg cctggttagg cttgtgtctg  10800 

ggctgggaga aagcaccacc ttgtggtagg agagagtact ggttgctcag ttaggactgg  10860 

gtagggcttt gcagttatcc ccaagtgtta tatacctcta ctcaccaacc tccttctccc  10920 

ctcaggcaag gcccgaggct tcttcaagaa gggagatgtg gtcattgtgc tgactggatg  10980 

gcgccctggc tctggcttca ccaacaccat gcgtgtagtg cctgtaccat gatgatcctc  11040 

tggagcttct cttctagccc ctgtcccttc ccctccccta tcctatccat taggccagca  11100 

acgttgtagt gctcactctg ggccatagtg tggcgctggt gggctgggac accaggaaaa  11160 

attaatgcct ctgaaacatg caatagagcc cagctatttt tcatggccct acttgagcca  11220 

ggggtgaagg aggaatgcag gattggaaac cctctgactt tatcacagaa gggcagcatt  11280 

atctctgtgt tctttgctcc tgtagaaagt tttccagaga attcccagcc ctggcctgga  11340 

atcaggagac agcaagaaca gaggctgggg gcccagggtt cccatgtaga tgacttttgg  11400 

ccctgtccct gacttgcttt cccaacagct ttggcctctc tcctcgtgca ctccactgct  11460 

gtccctgcag atgttccact ctccacctcg tactctgcag cgtctccagg cctgttgcta  11520 

tagtgcccac ctgaatgtca ataaacagca gcggaacgac ctgtgttctt ttcttcctgg  11580 

ggggcgtggt tgaggctatc ctctgagcag tcgaaaatga caaccggcct aaaggctcag  11640 

cctggagccg agtatatagg ttgcctcctc caacagcacc aagtggggct atctatgtgt  11700 

tggaaagctc actgttttat ttcaggacag actggaaagg tctgggacag gttcagctag  11760 

cacctgtaat ggtgggtaga ccggaggg                                     11788 

 
           
             4  
             446  
             PRT  
             Rattus norvegicus  
             
               Dopamine receptor D1  
             
           
            4 

Met Ala Pro Asn Thr Ser Thr Met Asp Glu Ala Gly Leu Pro Ala Glu 
1               5                   10                  15 

Arg Asp Phe Ser Phe Arg Ile Leu Thr Ala Cys Phe Leu Ser Leu Leu 
            20                  25                  30 

Ile Leu Ser Thr Leu Leu Gly Asn Thr Leu Val Cys Ala Ala Val Ile 
        35                  40                  45 

Arg Phe Arg His Leu Arg Ser Lys Val Thr Asn Phe Phe Val Ile Ser 
    50                  55                  60 

Leu Ala Val Ser Asp Leu Leu Val Ala Val Leu Val Met Pro Trp Lys 
65                  70                  75                  80 

Ala Val Ala Glu Ile Ala Gly Phe Trp Pro Phe Gly Ser Phe Cys Asn 
                85                  90                  95 

Ile Trp Val Ala Phe Asp Ile Met Cys Ser Thr Ala Ser Ile Leu Asn 
            100                 105                 110 

Leu Cys Val Ile Ser Val Asp Arg Tyr Trp Ala Ile Ser Ser Pro Phe 
        115                 120                 125 

Gln Tyr Glu Arg Lys Met Thr Pro Lys Ala Ala Phe Ile Leu Ile Ser 
    130                 135                 140 

Val Ala Trp Thr Leu Ser Val Leu Ile Ser Phe Ile Pro Val Gln Leu 
145                 150                 155                 160 

Ser Trp His Lys Ala Lys Pro Thr Trp Pro Leu Asp Gly Asn Phe Thr 
                165                 170                 175 

Ser Leu Glu Asp Thr Glu Asp Asp Asn Cys Asp Thr Arg Leu Ser Arg 
            180                 185                 190 

Thr Tyr Ala Ile Ser Ser Ser Leu Ile Ser Phe Tyr Ile Pro Val Ala 
        195                 200                 205 

Ile Met Ile Val Thr Tyr Thr Ser Ile Tyr Arg Ile Ala Gln Lys Gln 
    210                 215                 220 

Ile Arg Arg Ile Ser Ala Leu Glu Arg Ala Ala Val His Ala Lys Asn 
225                 230                 235                 240 

Cys Gln Thr Thr Ala Gly Asn Gly Asn Pro Val Glu Cys Ala Gln Ser 
                245                 250                 255 

Glu Ser Ser Phe Lys Met Ser Phe Lys Arg Glu Thr Lys Val Leu Lys 
            260                 265                 270 

Thr Leu Ser Val Ile Met Gly Val Phe Val Cys Cys Trp Leu Pro Phe 
        275                 280                 285 

Phe Ile Ser Asn Cys Met Val Pro Phe Cys Gly Ser Glu Glu Thr Gln 
    290                 295                 300 

Pro Phe Cys Ile Asp Ser Ile Thr Phe Asp Val Phe Val Trp Phe Gly 
305                 310                 315                 320 

Trp Ala Asn Ser Ser Leu Asn Pro Ile Ile Tyr Ala Phe Asn Ala Asp 
                325                 330                 335 

Phe Gln Lys Ala Phe Ser Thr Leu Leu Gly Cys Tyr Arg Leu Cys Pro 
            340                 345                 350 

Thr Thr Asn Asn Ala Ile Glu Thr Val Ser Ile Asn Asn Asn Gly Ala 
        355                 360                 365 

Val Val Phe Ser Ser His His Glu Pro Arg Gly Ser Ile Ser Lys Asp 
    370                 375                 380 

Cys Asn Leu Val Tyr Leu Ile Pro His Ala Val Gly Ser Ser Glu Asp 
385                 390                 395                 400 

Leu Lys Lys Glu Glu Ala Gly Gly Ile Ala Lys Pro Leu Glu Lys Leu 
                405                 410                 415 

Ser Pro Ala Leu Ser Val Ile Leu Asp Tyr Asp Thr Asp Val Ser Leu 
            420                 425                 430 

Glu Lys Ile Gln Pro Val Thr His Ser Gly Gln His Ser Thr 
        435                 440                 445 

 
           
             5  
             960  
             PRT  
             Mus musculus  
             
               GABA-B1a receptor  
             
           
            5 

Met Leu Leu Leu Leu Leu Leu Leu Leu Phe Leu Arg Pro Leu Gly Ala 
1               5                   10                  15 

Gly Gly Ala Gln Thr Pro Asn Val Thr Ser Glu Gly Cys Gln Ile Ile 
            20                  25                  30 

His Pro Pro Trp Glu Gly Gly Ile Arg Tyr Arg Gly Leu Ile Arg Asp 
        35                  40                  45 

Gln Val Lys Ala Ile Asn Phe Leu Pro Val Asp Tyr Glu Ile Glu Tyr 
    50                  55                  60 

Val Cys Arg Gly Glu Arg Glu Val Val Gly Pro Lys Val Arg Lys Cys 
65                  70                  75                  80 

Leu Ala Asn Gly Ser Trp Thr Asp Met Asp Thr Pro Ser Arg Cys Val 
                85                  90                  95 

Arg Ile Cys Ser Lys Ser Tyr Leu Thr Leu Glu Asn Gly Lys Val Phe 
            100                 105                 110 

Leu Thr Gly Gly Asp Leu Pro Ala Leu Asp Gly Ala Arg Val Asp Phe 
        115                 120                 125 

Arg Cys Asp Pro Asp Phe His Leu Val Gly Ser Ser Arg Ser Ile Cys 
    130                 135                 140 

Ser Gln Gly Gln Trp Ser Thr Pro Lys Pro His Cys Gln Val Asn Arg 
145                 150                 155                 160 

Thr Pro His Ser Glu Arg Arg Ala Val Tyr Ile Gly Ala Leu Phe Pro 
                165                 170                 175 

Met Ser Gly Gly Trp Pro Gly Gly Gln Ala Cys Gln Pro Ala Val Glu 
            180                 185                 190 

Met Ala Leu Glu Asp Val Asn Ser Arg Arg Asp Ile Leu Pro Asp Tyr 
        195                 200                 205 

Glu Leu Lys Leu Ile His His Asp Ser Lys Cys Asp Pro Gly Gln Ala 
    210                 215                 220 

Thr Lys Tyr Leu Tyr Glu Leu Leu Tyr Asn Asp Pro Ile Lys Ile Ile 
225                 230                 235                 240 

Leu Met Pro Gly Cys Ser Ser Val Ser Thr Leu Val Ala Glu Ala Ala 
                245                 250                 255 

Arg Met Trp Asn Leu Ile Val Leu Ser Tyr Gly Ser Ser Ser Pro Ala 
            260                 265                 270 

Leu Ser Asn Arg Gln Arg Phe Pro Thr Phe Phe Arg Thr His Pro Ser 
        275                 280                 285 

Ala Thr Leu His Asn Pro Thr Arg Val Lys Leu Phe Glu Lys Trp Gly 
    290                 295                 300 

Trp Lys Lys Ile Ala Thr Ile Gln Gln Thr Thr Glu Val Phe Thr Ser 
305                 310                 315                 320 

Thr Leu Asp Asp Leu Glu Glu Arg Val Lys Glu Ala Gly Ile Glu Ile 
                325                 330                 335 

Thr Phe Arg Gln Ser Phe Phe Ser Asp Pro Ala Val Pro Val Lys Asn 
            340                 345                 350 

Leu Lys Arg Gln Asp Ala Arg Ile Ile Val Gly Leu Phe Tyr Glu Thr 
        355                 360                 365 

Glu Ala Arg Lys Val Phe Cys Glu Val Tyr Lys Glu Arg Leu Phe Gly 
    370                 375                 380 

Lys Lys Tyr Val Trp Phe Leu Ile Gly Trp Tyr Ala Asp Asn Trp Phe 
385                 390                 395                 400 

Lys Thr Tyr Asp Pro Ser Ile Asn Cys Thr Val Glu Glu Met Thr Glu 
                405                 410                 415 

Ala Val Glu Gly His Ile Thr Thr Glu Ile Val Met Leu Asn Pro Ala 
            420                 425                 430 

Asn Thr Arg Ser Ile Ser Asn Met Thr Ser Gln Glu Phe Val Glu Lys 
        435                 440                 445 

Leu Thr Lys Arg Leu Lys Arg His Pro Glu Glu Thr Gly Gly Phe Gln 
    450                 455                 460 

Glu Ala Pro Leu Ala Tyr Asp Ala Ile Trp Ala Leu Ala Leu Ala Leu 
465                 470                 475                 480 

Asn Lys Thr Ser Gly Gly Gly Gly Arg Ser Gly Val Arg Leu Glu Asp 
                485                 490                 495 

Phe Asn Tyr Asn Asn Gln Thr Ile Thr Asp Gln Ile Tyr Arg Ala Met 
            500                 505                 510 

Asn Ser Ser Ser Phe Glu Gly Val Ser Gly His Val Val Phe Asp Ala 
        515                 520                 525 

Ser Gly Ser Arg Met Ala Trp Thr Leu Ile Glu Gln Leu Gln Gly Gly 
    530                 535                 540 

Ser Tyr Lys Lys Ile Gly Tyr Tyr Asp Ser Thr Lys Asp Asp Leu Ser 
545                 550                 555                 560 

Trp Ser Lys Thr Asp Lys Trp Ile Gly Gly Ser Pro Pro Ala Asp Gln 
                565                 570                 575 

Thr Leu Val Ile Lys Thr Phe Arg Phe Leu Ser Gln Lys Leu Phe Ile 
            580                 585                 590 

Ser Val Ser Val Leu Ser Ser Leu Gly Ile Val Leu Ala Val Val Cys 
        595                 600                 605 

Leu Ser Phe Asn Ile Tyr Asn Ser His Ala Arg Tyr Ile Gln Asn Ser 
    610                 615                 620 

Gln Pro Asn Leu Asn Asn Leu Thr Ala Val Gly Cys Ser Leu Ala Leu 
625                 630                 635                 640 

Ala Val Val Phe Pro Leu Gly Leu Asp Gly Tyr His Ile Gly Arg Ser 
                645                 650                 655 

Gln Phe Pro Phe Val Cys Gln Ala Arg Leu Trp Leu Leu Gly Leu Gly 
            660                 665                 670 

Phe Ser Leu Gly Tyr Gly Ser Met Phe Thr Lys Ile Trp Trp Val His 
        675                 680                 685 

Thr Val Phe Thr Lys Lys Glu Glu Lys Lys Glu Trp Arg Lys Thr Leu 
    690                 695                 700 

Glu Pro Trp Lys Leu Tyr Ala Thr Val Gly Leu Leu Val Gly Met Asp 
705                 710                 715                 720 

Val Leu Thr Leu Ala Ile Trp Gln Ile Val Asp Pro Leu His Arg Thr 
                725                 730                 735 

Ile Glu Thr Phe Ala Lys Glu Glu Pro Lys Glu Asp Ile Asp Val Ser 
            740                 745                 750 

Ile Leu Pro Gln Leu Glu His Cys Ser Ser Lys Lys Met Asn Thr Trp 
        755                 760                 765 

Leu Gly Ile Phe Tyr Gly Tyr Lys Gly Leu Leu Leu Leu Leu Gly Ile 
    770                 775                 780 

Phe Leu Ala Tyr Glu Thr Lys Ser Val Ser Thr Glu Lys Ile Asn Asp 
785                 790                 795                 800 

His Arg Ala Val Gly Met Ala Ile Tyr Asn Val Ala Val Leu Cys Leu 
                805                 810                 815 

Ile Thr Ala Pro Val Thr Met Ile Leu Ser Ser Gln Gln Asp Ala Ala 
            820                 825                 830 

Phe Ala Phe Ala Ser Leu Ala Ile Val Phe Ser Ser Tyr Ile Thr Leu 
        835                 840                 845 

Val Val Leu Phe Val Pro Lys Met Arg Arg Leu Ile Thr Arg Gly Glu 
    850                 855                 860 

Trp Gln Ser Glu Thr Gln Asp Thr Met Lys Thr Gly Ser Ser Thr Asn 
865                 870                 875                 880 

Asn Asn Glu Glu Glu Lys Ser Arg Leu Leu Glu Lys Glu Asn Arg Glu 
                885                 890                 895 

Leu Glu Lys Ile Ile Ala Glu Lys Glu Glu Arg Val Ser Glu Leu Arg 
            900                 905                 910 

His Gln Leu Gln Ser Arg Gln Gln Leu Arg Ser Arg Arg His Pro Pro 
        915                 920                 925 

Thr Pro Pro Asp Pro Ser Gly Gly Leu Pro Arg Gly Pro Ser Glu Pro 
    930                 935                 940 

Pro Asp Arg Leu Ser Cys Asp Gly Ser Arg Val His Leu Leu Tyr Lys 
945                 950                 955                 960 

 
           
             6  
             4365  
             DNA  
             Mus musculus  
             
               GABA-B1a receptor  
             
           
            6 

taacttgcgg ccgccgtcgc cccggggaag gagagacggg ggtggggttt gcgggtagcg     60 

atcgcgaagg ggagagaccc cggccgggct ggagcctgga ttcctgtgga agaagaacag    120 

ggggagggga agctggagga ccgggaggga gaacggggag ccgcggccgg gcctgggccc    180 

ttgaggcccg gggagagccg cggagcggga ccggccgccg agatgctgct gctgctgctt    240 

ctgcttctct tcctccgccc cctgggcgct ggcggggctc agacccccaa cgtcacctcg    300 

gaaggttgcc agattataca tccgccctgg gaaggtggca tcaggtaccg tggcttgatt    360 

cgcgaccagg tgaaggccat caatttcctg cctgtggact atgagattga atatgtgtgc    420 

cggggcgaac gcgaggtggt ggggcccaag gtgcgcaagt gcctggccaa cggctcctgg    480 

acggatatgg acacacccag tcgctgtgtc cgaatctgct ccaagtctta tttgaccctg    540 

gaaaatggga aggttttcct gacgggtggg gacctcccag ctctggatgg agcccgggtg    600 

gatttccgat gtgaccctga cttccatctg gtgggcagct cccggagcat ctgtagtcag    660 

ggccagtgga gcacccccaa gccccactgc caggtgaatc gaacgccaca ctcagaacgg    720 

cgtgcagtat acatcggggc gctgtttccc atgagcgggg gctggccggg gggccaggcc    780 

tgccagcctg cggtggagat ggcgctggag gacgttaaca gccgcagaga catcctgccg    840 

gactacgagc tcaagcttat ccaccacgac agcaagtgcg acccagggca agccaccaag    900 

tacttgtatg aactactcta caacgacccc atcaagatca tcctcatgcc cggctgcagc    960 

tctgtgtcca cactggtagc cgaggctgcc cggatgtgga accttattgt gctctcatat   1020 

ggctccagct caccagcctt gtcaaaccga cagcggtttc caacgttctt tcggacacat   1080 

ccatccgcca cactccacaa tcccacccgg gtgaaactct tcgaaaagtg gggctggaag   1140 

aagattgcca ccatccagca gactaccgag gtcttcacct caacactgga tgacctggag   1200 

gagcgagtga aagaggctgg gattgagatc acttttcgac agagtttctt ctcagatcca   1260 

gctgtgcctg ttaaaaacct gaagcgtcaa gatgctcgaa tcatcgtggg acttttctat   1320 

gagaccgaag cccggaaagt tttttgtgag gtctataagg aacggctctt tgggaagaag   1380 

tatgtctggt ttctcatcgg gtggtatgct gacaactggt tcaaaaccta tgacccgtca   1440 

atcaattgta cagtagaaga gatgactgag gcggtggagg gccatatcac cacggagatt   1500 

gtcatgctga accctgccaa cacccgaagc atttccaaca tgacatcaca ggaatttgtg   1560 

gagaaactaa ccaagcggct gaaaagacac cctgaggaga ctggaggctt ccaggaggca   1620 

ccactggcct atgatgctat ttgggccttg gctttggcct tgaacaagac ctctggagga   1680 

ggtggccgtt caggagtgcg cctggaggac tttaactaca acaaccagac cattacagac   1740 

caaatctacc gggccatgaa ctcctcctcc tttgagggtg tttctggcca cgtggtcttt   1800 

gatgccagcg gctcccggat ggcatggacg cttatcgagc agctacaggg cggcagctac   1860 

aagaagatcg gctactacga cagcaccaag gatgatcttt cctggtccaa aacagacaag   1920 

tggatcggag ggtctccccc agccgaccag accttggtca tcaagacatt ccgtttcctg   1980 

tcacagaaac tctttatctc cgtctcagtt ctctccagcc tgggcattgt tcttgctgtt   2040 

gtctgtctgt cctttaacat ctacaactcc cacgctcgtt atatccagaa ttcccagccc   2100 

aacctgaaca atctgactgc tgtgggctgc tcactggcac tggctgttgt cttccctctc   2160 

gggctggatg gttaccacat agggagaagc cagttcccgt ttgtctgcca ggcccgcctt   2220 

tggctcttgg gcttgggctt tagtctgggc tatggctcta tgttcaccaa gatctggtgg   2280 

gtccacacag tcttcacgaa gaaggaggag aagaaggagt ggaggaagac cctagagccc   2340 

tggaaactct atgccactgt gggcctgctg gtgggcatgg atgtcctgac tcttgccatc   2400 

tggcagattg tggacccctt gcaccgaacc attgagactt ttgccaagga ggaaccaaag   2460 

gaagacatcg atgtctccat tctgccccag ttggagcact gcagctccaa gaagatgaat   2520 

acgtggcttg gcattttcta tggttacaag gggctgctgc tgctgctggg aatctttctt   2580 

gcttacgaaa ccaagagcgt gtccactgaa aagatcaatg accacagggc cgtgggcatg   2640 

gctatctaca atgtcgcggt cctgtgtctc atcactgctc ctgtgaccat gatcctttcc   2700 

agtcagcagg acgcagcctt tgcctttgcc tctctggcca tcgtgttctc ttcctacatc   2760 

actctggttg tgctctttgt gcccaagatg cgcaggctga tcacccgagg ggaatggcag   2820 

tctgaaacgc aggacaccat gaaaacagga tcatccacca acaacaacga ggaagagaag   2880 

tcccgactgt tggagaagga aaaccgagaa ctggaaaaga tcatcgctga gaaagaggag   2940 

cgcgtctctg aactgcgcca tcagctccag tctcggcagc aactccgctc acggcgccac   3000 

cccccaacac ccccagatcc ctctgggggc cttcccaggg gaccctctga gccccctgac   3060 

cggcttagct gtgatgggag tcgagtacat ttgctttaca agtgaggggg catggagaag   3120 

gatcaagcca gtaggggagg gaagggtctg ggaagagggt gggggcctgg gaggagggta   3180 

aggactccta tctccaacct ggagagcaca cgctccaatc cccctcttat aaatacatgt   3240 

cgctctgtgc atctggggtt atttgggtct ccagtactct gggaaacaga ctgttttctt   3300 

tctcccctat aattttatat ctccacttca caggttttgt ttgaaccctg cttggagtta   3360 

ttattcactc atggctccag aggggcatct catttttctc cggtagcctg tcttgtacag   3420 

ttaccacagc aactcctgtc atttcagcag caggggtctt cctacactag cagggctctc   3480 

gctctctcca tttttcagcc tcagaatctc cttccattat tcttctcctt ctacatgtct   3540 

ccatggcttc ctctcccagg ggactcgttc tacacacata cacacacaca cacacacaca   3600 

cacacacaca cacacacaca cacaccccgc atcctgccct ctcctaggca gctgcatgtc   3660 

gtcctgtaca aatgtgctcg cttctgagtg ctttgtgcgg ccgttcactt gtgctgtctg   3720 

cataagctgc gtctgtgagt gcacggtggt ttgtgggtgc gtgaagtggc atgctccggt   3780 

aggtgtgtgt gtgtgtgccc acgcgtgcgc ccgtgtgcat gcgttcgtgt tgccctgact   3840 

ggctgtctca gccttctgag taattgggat tccagttgtc tgtctagctc atgtcctgtc   3900 

ttcttccagt agagccgtga acacccaaca cacacagtta atcgggctcc ccccagtcca   3960 

tgttttctga gccatccaaa aactctcctt ggccttaggt tcatctgcaa atgttccctc   4020 

tgttctttgc tctcgtgcgt ccaccttcat tctcttcagt catttctcag atctgctgcg   4080 

tcgtggtttc ctttccttca ttatcatcgt cattattttt cagaacttaa gggaaaaaga   4140 

aatggggaca ggttggaggc tgtttccagt ggaatagtgg gtgcgcgtcc tgaccaaatg   4200 

aaggcacgga cagatggact gacggggcgg gaggcggcgt ccctttcaca ccgtggtgtc   4260 

tcttgggggg gaaggatctc cctgaatctc aataaagcag tgaacagtaa aaaaaaaaaa   4320 

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa                   4365 

 
           
             7  
             582  
             PRT  
             Rattus norvegicus  
             
               Differentiation-associated Na-dependent 
      inorganic phosphate cotransporter  
             
           
            7 

Met Glu Ser Val Lys Gln Arg Ile Leu Ala Pro Gly Lys Glu Gly Ile 
1               5                   10                  15 

Lys Asn Phe Ala Gly Lys Ser Leu Gly Gln Ile Tyr Arg Val Leu Glu 
            20                  25                  30 

Lys Lys Gln Asp Asn Arg Glu Thr Ile Glu Leu Thr Glu Asp Gly Lys 
        35                  40                  45 

Pro Leu Glu Val Pro Glu Lys Lys Ala Pro Leu Cys Asp Cys Thr Cys 
    50                  55                  60 

Phe Gly Leu Pro Arg Arg Tyr Ile Ile Ala Ile Met Ser Gly Leu Gly 
65                  70                  75                  80 

Phe Cys Ile Ser Phe Gly Ile Arg Cys Asn Leu Gly Val Ala Ile Val 
                85                  90                  95 

Asp Met Val Asn Asn Ser Thr Ile His Arg Gly Gly Lys Val Ile Lys 
            100                 105                 110 

Glu Lys Ala Lys Phe Asn Trp Asp Pro Glu Thr Val Gly Met Ile His 
        115                 120                 125 

Gly Ser Phe Phe Trp Gly Tyr Ile Ile Thr Gln Ile Pro Gly Gly Tyr 
    130                 135                 140 

Ile Ala Ser Arg Leu Ala Ala Asn Arg Val Phe Gly Ala Ala Ile Leu 
145                 150                 155                 160 

Leu Thr Ser Thr Leu Asn Met Leu Ile Pro Ser Ala Ala Arg Val His 
                165                 170                 175 

Tyr Gly Cys Val Ile Phe Val Arg Ile Leu Gln Gly Leu Val Glu Gly 
            180                 185                 190 

Val Thr Tyr Pro Ala Cys His Gly Ile Trp Ser Lys Trp Ala Pro Pro 
        195                 200                 205 

Leu Glu Arg Ser Arg Leu Ala Thr Thr Ser Phe Cys Gly Ser Tyr Ala 
    210                 215                 220 

Gly Ala Val Ile Ala Met Pro Leu Ala Gly Ile Leu Val Gln Tyr Thr 
225                 230                 235                 240 

Gly Trp Ser Ser Val Phe Tyr Val Tyr Gly Ser Phe Gly Met Val Trp 
                245                 250                 255 

Tyr Met Phe Trp Leu Leu Val Ser Tyr Glu Ser Pro Ala Lys His Pro 
            260                 265                 270 

Thr Ile Thr Asp Glu Glu Arg Arg Tyr Ile Glu Glu Ser Ile Gly Glu 
        275                 280                 285 

Ser Ala Asn Leu Leu Gly Ala Met Glu Lys Phe Lys Thr Pro Trp Arg 
    290                 295                 300 

Lys Phe Phe Thr Ser Met Pro Val Tyr Ala Ile Ile Val Ala Asn Phe 
305                 310                 315                 320 

Cys Arg Ser Trp Thr Phe Tyr Leu Leu Leu Ile Ser Gln Pro Ala Tyr 
                325                 330                 335 

Phe Glu Glu Val Phe Gly Phe Glu Ile Ser Lys Val Gly Met Leu Ser 
            340                 345                 350 

Ala Val Pro His Leu Val Met Thr Ile Ile Val Pro Ile Gly Gly Gln 
        355                 360                 365 

Ile Ala Asp Phe Leu Arg Ser Lys Gln Ile Leu Ser Thr Thr Thr Val 
    370                 375                 380 

Arg Lys Ile Met Asn Cys Gly Gly Phe Gly Met Glu Ala Thr Leu Leu 
385                 390                 395                 400 

Leu Val Val Gly Tyr Ser His Thr Arg Gly Val Ala Ile Ser Phe Leu 
                405                 410                 415 

Val Leu Ala Val Gly Phe Ser Gly Phe Ala Ile Ser Gly Phe Asn Val 
            420                 425                 430 

Asn His Leu Asp Ile Ala Pro Arg Tyr Ala Ser Ile Leu Met Gly Ile 
        435                 440                 445 

Ser Asn Gly Val Gly Thr Leu Ser Gly Met Val Cys Pro Ile Ile Val 
    450                 455                 460 

Gly Ala Met Thr Lys Asn Lys Ser Arg Glu Glu Trp Gln Tyr Val Phe 
465                 470                 475                 480 

Leu Ile Ala Ala Leu Val His Tyr Gly Gly Val Ile Phe Tyr Ala Leu 
                485                 490                 495 

Phe Ala Ser Gly Glu Lys Gln Pro Trp Ala Asp Pro Glu Glu Thr Ser 
            500                 505                 510 

Glu Glu Lys Cys Gly Phe Ile His Glu Asp Glu Leu Asp Glu Glu Thr 
        515                 520                 525 

Gly Asp Ile Thr Gln Asn Tyr Ile Asn Tyr Gly Thr Thr Lys Ser Tyr 
    530                 535                 540 

Gly Ala Thr Ser Gln Glu Asn Gly Gly Trp Pro Asn Gly Trp Glu Lys 
545                 550                 555                 560 

Lys Glu Glu Phe Val Gln Glu Ser Ala Gln Asp Ala Tyr Ser Tyr Lys 
                565                 570                 575 

Asp Arg Asp Asp Tyr Ser 
            580 

 
           
             8  
             3982  
             DNA  
             Rattus norvegicus  
             
               Differentiation-associated Na-dependent 
      inorganic phosphate cotransporter  
             
           
            8 

agacagtaag gttcttttgc ttttttccct tacacaagga ttcgatgacg tttttggtca     60 

atctgattaa aagacagcgg atttggttgc gttaagactt caaaaccggg aatttacgtt    120 

gtttttcggt gaggtgactt ccagaacggg gactcatcag cacccgccca aataccacgg    180 

cactgcgcgc gccctcggcc accggatcct ccccttccaa tgagactttg tgactgtgtg    240 

taccaattct cctattagga aacccgtggg ctgaatgcag ctattccgtt gtactctctt    300 

tctcgctctc cctcccctct ccaactcaca gccttgctga aaagctcatc tctgctgaga    360 

agaaaacgtt ctaccttaac ctattaagac tatgcgcaga actcgccttt catagccatc    420 

acaatttaaa tctggtaagg ctggacacga gtctttacaa gaatggagtc ggtaaaacaa    480 

aggattttgg ccccggggaa agaggggata aagaattttg ctggaaaatc cctcggacag    540 

atctacaggg tgctggagaa gaagcaggat aaccgagaga ccatcgagct gacagaggac    600 

ggcaagcccc tggaggtgcc tgagaagaag gctccgctat gcgactgtac gtgcttcggc    660 

ctgccgcgcc gctacatcat agccatcatg agcggcctcg gcttctgcat ctcctttggt    720 

atccgctgta acctgggtgt ggccattgtg gacatggtca acaacagcac catccaccgg    780 

ggtggcaaag ttatcaagga gaaagccaag tttaactggg accccgagac tgtggggatg    840 

attcacgggt cgttcttctg gggctatatc atcacgcaga ttccgggcgg atacatcgca    900 

tcgcgactgg ctgctaaccg ggtctttggg gctgccatac tgcttacctc taccctcaat    960 

atgctgatcc catctgcagc cagagtgcat tatggatgcg tcatctttgt tagaatattg   1020 

caaggacttg tggagggcgt cacctaccca gcctgtcacg ggatatggag caagtgggcc   1080 

cctcctttgg agaggagtag gttggctacc acctccttct gtggttccta tgctggagca   1140 

gtcattgcaa tgcccctagc tggtatcctg gtgcagtaca ctggatggtc ttcagtattt   1200 

tacgtatatg gaagctttgg tatggtctgg tatatgttct ggcttctggt gtcttacgag   1260 

agccccgcaa agcatccaac cataacagac gaagaacgta ggtacataga agagagcatc   1320 

ggggagagcg caaatctgtt aggagcaatg gagaaattca agaccccatg gaggaagttt   1380 

ttcacatcca tgcccgtcta tgcgataatt gttgcaaact tctgcaggag ttggactttt   1440 

tatttactgc tcatcagtca accagcttat ttcgaggagg tttttggatt tgaaatcagc   1500 

aaggttggca tgttgtctgc ggtcccacac ctggtcatga caatcattgt gcctatcggg   1560 

gggcaaattg cagactttct aaggagcaag caaattcttt caacaactac agtgcgaaag   1620 

atcatgaact gcgggggttt tggcatggaa gccacactgc ttctggttgt tggctactct   1680 

catactagag gggtggccat ctccttcttg gtgcttgcag tgggattcag tggatttgct   1740 

atctctggtt tcaatgtgaa ccacttggat attgccccga gatatgccag tatcttaatg   1800 

ggcatttcaa atggtgttgg cacgctgtcg ggaatggtct gcccgatcat tgttggtgca   1860 

atgacgaaga acaagtcccg tgaagaatgg cagtatgtct tcctcatcgc tgcactggtc   1920 

cactatggtg gagtcatatt ttatgcacta tttgcctcag gagagaagca accttgggca   1980 

gaccctgagg aaacaagcga agaaaagtgt ggcttcattc atgaagatga actggatgaa   2040 

gaaacggggg acatcactca gaattacata aattacggta ccaccaaatc ctacggcgcc   2100 

acctcacagg agaacggagg ctggcctaac ggctgggaga aaaaggaaga atttgtgcaa   2160 

gaaagtgcgc aagacgcgta ctcctataag gaccgagatg attattcata acgaagctag   2220 

ttgctggatt cctttgtagt gtttgtgatt aaattaattg tgattgcaca aaatcatttt   2280 

aagaaatgtg gtgtaaacat gtaaacacat caaccaagca agtcttgctg ttcaaaaaat   2340 

aataataata tgaattcaaa acagaccgtg agagtcccat caagtgcaat ctgtggcggc   2400 

agtcacgtga cgccatttcc attcaggcca ttcgtccttt tcgtttgtga tttaaaggtt   2460 

tcctgtagaa ataagtaggt attcgttgga tccatcacca cgttagagag tacaactaca   2520 

acagttggca catgtcatcc tacggaagtt aggaagccaa agctactgga ttatgtgaac   2580 

tgcatttatt tatttattac actggactgc aaaatatccc agggaaatcc tgtctagaga   2640 

catagtagaa ctggaaagat ggctagattg ggtactgacg ataatcattg tgtgtatatc   2700 

atggagtggc tatatctttt aattggagaa ctatattgta tagctagcaa aattgtactg   2760 

aattattact aggagtgcac agtgtgtgat attttgtgat cttccaaaag cttatcttgc   2820 

agtgttttgt gaaacgcttg ggcacaaaca cttattttta tgaacaagag cttgtaaagg   2880 

gaggagtatg ctccatgctc tcccattcac tacctgacag tatcaaacct tcacatttca   2940 

atgaaatcca acgtccatgt aacatatcac atgacttttt ttgcaaaaaa gaatataaga   3000 

agaaatagac ttcaatgtat tttttattac aactttgtac tggttgtaac ttgcattagg   3060 

aaaaatgatt aatatatgta taatcgtaaa gaatctaata aaaatttact atgaagatat   3120 

agcccttaaa atgcaatatt aacaacaaaa atatatagaa aatttagata atcttccttg   3180 

ataactagag actatatgga actcacacca caaagctata tataatatga aaagataaac   3240 

aatagagatt gtatatgtag acgattttat gacctaatgt cccatttaag aggtatttgt   3300 

cttgagtata tagtacaaag tatattaaaa ttatatctac atccctgtat atcttataca   3360 

tatccactca cacaaacata acaaatactt ttcacacaga accaaaaaca agcatacacc   3420 

taatgttggg tttggggatt gcaatttcta ctttcataga gtcatagaat tttagatggg   3480 

aaaaaaaaag gcattttgct cgtcatttct taatataatt aattcaacag gaactgcaac   3540 

atttgtgtac caagcaataa gtgcgaagca taaacctgct gtgtgtaaac tatccccata   3600 

ctgcttgtgg tagcactgat ttctttcttt taaagaactt aacatcggag ctctttacaa   3660 

tgttttgcgc tgataagaat gcacatccca atttaacgca aagtgtcacc tggtgtgttt   3720 

acctgtctgt tttgggtatt tggtctgttt ggtgtcctgt gctcttgact ggaggccctg   3780 

ctactgcgaa tataaaacgt gaagtttgtt tctaaatgca aaccactcct gaccttaaga   3840 

aactaaagtc cctctctgct ttgtgtctcc aagtactatc atgtgaccat aacccttgct   3900 

gtgctgagta aaaagatgtg aactgtcatt ttgttgctgc gaagcaagtg ttaataaaat   3960 

gttctattta aaaaaaaaaa aa                                            3982 

 
           
             9  
             854  
             PRT  
             Homo sapiens  
             
               Vacuolar assembly protein VSP41 gene  
             
           
            9 

Met Ala Glu Ala Val Glu Gln Glu Thr Gly Ser Leu Glu Glu Ser Thr 
1               5                   10                  15 

Asp Glu Ser Glu Glu Glu Glu Ser Glu Glu Glu Pro Lys Leu Lys Tyr 
            20                  25                  30 

Glu Arg Leu Ser Asn Gly Val Thr Glu Ile Leu Gln Lys Asp Ala Ala 
        35                  40                  45 

Ser Cys Met Thr Val His Asp Lys Phe Leu Ala Leu Gly Thr His Tyr 
    50                  55                  60 

Gly Lys Val Tyr Leu Leu Asp Val Gln Gly Asn Ile Thr Gln Lys Phe 
65                  70                  75                  80 

Asp Val Ser Pro Val Lys Ile Asn Gln Ile Ser Leu Asp Glu Ser Gly 
                85                  90                  95 

Glu His Met Gly Val Cys Ser Glu Asp Gly Lys Val Gln Val Phe Gly 
            100                 105                 110 

Leu Tyr Ser Gly Glu Glu Phe His Glu Thr Phe Asp Cys Pro Ile Lys 
        115                 120                 125 

Ile Ile Ala Val His Pro His Phe Val Arg Ser Ser Cys Lys Gln Phe 
    130                 135                 140 

Val Thr Gly Gly Lys Lys Leu Leu Leu Phe Glu Arg Ser Trp Met Asn 
145                 150                 155                 160 

Arg Trp Lys Ser Ala Val Leu His Glu Gly Glu Gly Asn Ile Arg Ser 
                165                 170                 175 

Val Lys Trp Arg Gly His Leu Ile Ala Trp Ala Asn Asn Met Gly Val 
            180                 185                 190 

Lys Ile Phe Asp Ile Ile Ser Lys Gln Arg Ile Thr Asn Val Pro Arg 
        195                 200                 205 

Asp Asp Ile Ser Leu Arg Pro Asp Met Tyr Pro Cys Ser Leu Cys Trp 
    210                 215                 220 

Lys Asp Asn Val Thr Leu Ile Ile Gly Trp Gly Thr Ser Val Lys Val 
225                 230                 235                 240 

Cys Ser Val Lys Glu Arg His Ala Ser Glu Met Arg Asp Leu Pro Ser 
                245                 250                 255 

Arg Tyr Val Glu Ile Val Ser Gln Phe Glu Thr Glu Phe Tyr Ile Ser 
            260                 265                 270 

Gly Leu Ala Pro Leu Cys Asp Gln Leu Val Val Leu Ser Tyr Val Lys 
        275                 280                 285 

Glu Ile Ser Glu Lys Thr Glu Arg Glu Tyr Cys Ala Arg Pro Arg Leu 
    290                 295                 300 

Asp Ile Ile Gln Pro Leu Ser Glu Thr Cys Glu Glu Ile Ser Ser Asp 
305                 310                 315                 320 

Ala Leu Thr Val Arg Gly Phe Gln Glu Asn Glu Cys Arg Asp Tyr His 
                325                 330                 335 

Leu Glu Tyr Ser Glu Gly Glu Ser Leu Phe Tyr Ile Val Ser Pro Arg 
            340                 345                 350 

Asp Val Val Val Ala Lys Glu Arg Asp Gln Asp Asp His Ile Asp Trp 
        355                 360                 365 

Leu Leu Glu Lys Lys Lys Tyr Glu Glu Ala Leu Met Ala Ala Glu Ile 
    370                 375                 380 

Ser Gln Lys Asn Ile Lys Arg His Lys Ile Leu Asp Ile Gly Leu Ala 
385                 390                 395                 400 

Tyr Ile Asn His Leu Val Glu Arg Gly Asp Tyr Asp Ile Ala Ala Arg 
                405                 410                 415 

Lys Cys Gln Lys Ile Leu Gly Lys Asn Ala Ala Leu Trp Glu Tyr Glu 
            420                 425                 430 

Val Tyr Lys Phe Lys Glu Ile Gly Gln Leu Lys Ala Ile Ser Pro Tyr 
        435                 440                 445 

Leu Pro Arg Gly Asp Pro Val Leu Lys Pro Leu Ile Tyr Glu Met Ile 
    450                 455                 460 

Leu His Glu Phe Leu Glu Ser Asp Tyr Glu Gly Phe Ala Thr Leu Ile 
465                 470                 475                 480 

Arg Glu Trp Pro Gly Asp Leu Tyr Asn Asn Ser Val Ile Val Gln Ala 
                485                 490                 495 

Val Arg Asp His Leu Lys Lys Asp Ser Gln Asn Lys Thr Leu Leu Lys 
            500                 505                 510 

Thr Leu Ala Glu Leu Tyr Thr Tyr Asp Lys Asn Tyr Gly Asn Ala Leu 
        515                 520                 525 

Glu Ile Tyr Leu Thr Leu Arg His Lys Asp Val Phe Gln Leu Ile His 
    530                 535                 540 

Lys His Asn Leu Phe Ser Ser Ile Lys Asp Lys Ile Val Leu Leu Met 
545                 550                 555                 560 

Asp Phe Asp Ser Glu Lys Ala Val Asp Met Leu Leu Asp Asn Glu Asp 
                565                 570                 575 

Lys Ile Ser Ile Lys Lys Val Val Glu Glu Leu Glu Asp Arg Pro Glu 
            580                 585                 590 

Leu Gln His Val Tyr Leu His Lys Leu Phe Lys Arg Asp His His Lys 
        595                 600                 605 

Gly Gln Arg Tyr His Glu Lys Gln Ile Ser Leu Tyr Ala Glu Tyr Asp 
    610                 615                 620 

Arg Pro Asn Leu Leu Pro Phe Leu Arg Asp Ser Thr His Cys Pro Leu 
625                 630                 635                 640 

Glu Lys Ala Leu Glu Ile Cys Gln Gln Arg Asn Phe Val Glu Glu Thr 
                645                 650                 655 

Val Tyr Leu Leu Ser Arg Met Gly Asn Ser Arg Ser Ala Leu Lys Met 
            660                 665                 670 

Ile Met Glu Glu Leu His Asp Val Asp Lys Ala Ile Glu Phe Ala Lys 
        675                 680                 685 

Glu Gln Asp Asp Gly Glu Leu Trp Glu Asp Leu Ile Leu Tyr Ser Ile 
    690                 695                 700 

Asp Lys Pro Pro Phe Ile Thr Gly Leu Leu Asn Asn Ile Gly Thr His 
705                 710                 715                 720 

Val Asp Pro Ile Leu Leu Ile His Arg Ile Lys Glu Gly Met Glu Ile 
                725                 730                 735 

Pro Asn Leu Arg Asp Ser Leu Val Lys Ile Leu Gln Asp Tyr Asn Leu 
            740                 745                 750 

Gln Ile Leu Leu Arg Glu Gly Cys Lys Lys Ile Leu Val Ala Asp Ser 
        755                 760                 765 

Leu Ser Leu Leu Lys Lys Met His Arg Thr Gln Met Lys Gly Val Leu 
    770                 775                 780 

Val Asp Glu Glu Asn Ile Cys Glu Ser Cys Leu Ser Pro Ile Leu Pro 
785                 790                 795                 800 

Ser Asp Ala Ala Lys Pro Phe Ser Val Val Val Phe His Cys Arg His 
                805                 810                 815 

Met Phe His Lys Glu Cys Leu Pro Met Pro Ser Met Asn Ser Ala Ala 
            820                 825                 830 

Gln Phe Cys Asn Ile Cys Ser Ala Lys Asn Arg Gly Pro Gly Ser Ala 
        835                 840                 845 

Ile Leu Glu Met Lys Lys 
    850 

 
           
             10  
             770  
             PRT  
             Rattus norvegicus  
             
               Git 1  
             
           
            10 

Met Ser Arg Lys Gly Pro Arg Ala Glu Val Cys Ala Asp Cys Ser Ala 
1               5                   10                  15 

Pro Asp Pro Gly Trp Ala Ser Ile Ser Arg Gly Val Leu Val Cys Asp 
            20                  25                  30 

Glu Cys Cys Ser Val His Arg Ser Leu Gly Arg His Ile Ser Ile Val 
        35                  40                  45 

Lys His Leu Arg His Ser Ala Trp Pro Pro Thr Leu Leu Gln Met Val 
    50                  55                  60 

His Thr Leu Ala Ser Asn Gly Ala Asn Ser Ile Trp Glu His Ser Leu 
65                  70                  75                  80 

Leu Asp Pro Ala Gln Val Gln Ser Gly Arg Arg Lys Ala Asn Pro Gln 
                85                  90                  95 

Asp Lys Val His Pro Ile Lys Ser Glu Phe Ile Arg Ala Lys Tyr Gln 
            100                 105                 110 

Met Leu Ala Phe Val His Lys Leu Pro Cys Arg Asp Asp Asp Gly Val 
        115                 120                 125 

Thr Ala Lys Asp Leu Ser Lys Gln Leu His Ser Ser Val Arg Thr Gly 
    130                 135                 140 

Asn Leu Glu Thr Cys Leu Arg Leu Leu Ser Leu Gly Ala Gln Ala Asn 
145                 150                 155                 160 

Phe Phe His Pro Glu Lys Gly Thr Thr Pro Leu His Val Ala Ala Lys 
                165                 170                 175 

Ala Gly Gln Thr Leu Gln Ala Glu Leu Leu Val Val Tyr Gly Ala Asp 
            180                 185                 190 

Pro Gly Ser Pro Asp Val Asn Gly Arg Thr Pro Ile Asp Tyr Ala Arg 
        195                 200                 205 

Gln Ala Gly His His Glu Leu Ala Glu Arg Leu Val Glu Cys Gln Tyr 
    210                 215                 220 

Glu Leu Thr Asp Arg Leu Ala Phe Tyr Leu Cys Gly Arg Lys Pro Asp 
225                 230                 235                 240 

His Lys Asn Gly His Tyr Ile Ile Pro Gln Met Ala Asp Arg Ser Arg 
                245                 250                 255 

Gln Lys Cys Met Ser Gln Ser Leu Asp Leu Ser Glu Leu Ala Lys Ala 
            260                 265                 270 

Ala Lys Lys Lys Leu Gln Ala Leu Ser Asn Arg Leu Phe Glu Glu Leu 
        275                 280                 285 

Ala Met Asp Val Tyr Asp Glu Val Asp Arg Arg Glu Asn Asp Ala Val 
    290                 295                 300 

Trp Leu Ala Thr Gln Asn His Ser Thr Leu Val Thr Glu Arg Ser Ala 
305                 310                 315                 320 

Val Pro Phe Leu Pro Val Asn Pro Glu Tyr Ser Ala Thr Arg Asn Gln 
                325                 330                 335 

Gly Arg Gln Lys Leu Ala Arg Phe Asn Ala Arg Glu Phe Ala Thr Leu 
            340                 345                 350 

Ile Ile Asp Ile Leu Ser Glu Ala Lys Arg Arg Gln Gln Gly Lys Ser 
        355                 360                 365 

Leu Ser Ser Pro Thr Asp Asn Leu Glu Leu Ser Ala Arg Asn Gln Ser 
    370                 375                 380 

Asp Leu Asp Asp Gln His Asp Tyr Asp Ser Val Ala Ser Asp Glu Asp 
385                 390                 395                 400 

Thr Asp Gln Glu Pro Leu Pro Ser Ala Gly Ala Thr Arg Asn Asn Arg 
                405                 410                 415 

Ala Arg Ser Met Asp Ser Ser Asp Leu Ser Asp Gly Ala Val Thr Leu 
            420                 425                 430 

Gln Glu Tyr Leu Glu Leu Lys Lys Ala Leu Ala Thr Ser Glu Ala Lys 
        435                 440                 445 

Val Gln Gln Leu Met Lys Val Asn Ser Ser Leu Ser Asp Glu Leu Arg 
    450                 455                 460 

Lys Leu Gln Arg Glu Ile His Lys Leu Gln Ala Glu Asn Leu Gln Leu 
465                 470                 475                 480 

Arg Gln Pro Pro Gly Pro Val Pro Val Pro Ser Leu Pro Ser Glu Arg 
                485                 490                 495 

Ala Glu His Thr Leu Met Gly Pro Gly Gly Ser Thr His Arg Arg Asp 
            500                 505                 510 

Arg Gln Ala Phe Ser Met Tyr Glu Pro Gly Ser Ala Leu Lys Pro Phe 
        515                 520                 525 

Gly Gly Ala Pro Gly Asp Glu Leu Ala Thr Arg Leu Gln Pro Phe His 
    530                 535                 540 

Ser Thr Glu Leu Glu Asp Asp Ala Ile Tyr Ser Val His Val Pro Ala 
545                 550                 555                 560 

Gly Leu Tyr Arg Ile Arg Lys Gly Val Ser Ala Ser Ser Val Thr Phe 
                565                 570                 575 

Thr Pro Ser Ser Pro Leu Leu Ser Ser Ser Gln Glu Gly Ser Arg His 
            580                 585                 590 

Ala Ser Lys Leu Ser Arg His Gly Ser Gly Ala Glu Ser Asp Tyr Glu 
        595                 600                 605 

Asn Thr Gln Ser Gly Glu Pro Leu Leu Gly Leu Glu Gly Lys Arg Phe 
    610                 615                 620 

Leu Glu Leu Ser Lys Glu Asp Glu Leu His Ala Glu Leu Glu Ser Leu 
625                 630                 635                 640 

Asp Gly Asp Pro Asp Pro Gly Leu Pro Ser Thr Glu Asp Val Ile Leu 
                645                 650                 655 

Lys Thr Glu Gln Val Thr Lys Asn Ile Gln Glu Leu Leu Arg Ala Ala 
            660                 665                 670 

Gln Glu Phe Lys His Asp Ser Phe Val Pro Cys Ser Glu Lys Ile His 
        675                 680                 685 

Leu Ala Val Thr Glu Met Ala Ser Leu Phe Pro Lys Arg Pro Ala Leu 
    690                 695                 700 

Glu Pro Val Arg Ser Ser Leu Arg Leu Leu Asn Ala Ser Ala Tyr Arg 
705                 710                 715                 720 

Leu Gln Ser Glu Cys Arg Lys Thr Val Pro Pro Glu Pro Gly Ala Pro 
                725                 730                 735 

Val Asp Phe Gln Leu Leu Thr Gln Gln Val Ile Gln Cys Ala Tyr Asp 
            740                 745                 750 

Ile Ala Lys Ala Ala Lys Gln Leu Val Thr Ile Thr Thr Arg Glu Lys 
        755                 760                 765 

Lys Gln 
    770 

 
           
             11  
             3236  
             DNA  
             Rattus norvegicus  
             
               Git 1  
             
           
            11 

gcccgcgccc cccacgctgg cccagagggc tgcggccgct tcgccgctga ggatgtcccg     60 

gaaggggccg cgagcggagg tgtgtgcgga ctgcagcgcc cctgaccctg gctgggcatc    120 

tatcagcaga ggtgtgctgg tttgtgacga gtgctgcagt gtgcaccgga gcctgggacg    180 

acacatctcc attgtcaaac accttcgcca cagcgcctgg cctcctacgc tgctacagat    240 

ggtgcacacg cttgccagca acggggccaa ctctatctgg gagcattccc tgctggaccc    300 

tgcacaagtg caaagtggcc ggcgcaaagc caacccccaa gacaaagtcc accccatcaa    360 

gtcagagttc atcagggcaa agtaccagat gctggcgttt gtgcacaagc ttccctgccg    420 

tgatgatgat ggggtcaccg ccaaagacct cagcaagcaa ctgcactcga gtgtgcgaac    480 

aggcaacctg gagacctgtc tgcgcctgct ttccctgggt gcccaggcca acttcttcca    540 

cccagaaaag ggcactacac ctctacacgt ggctgccaag gcagggcaga cactgcaggc    600 

tgagctgcta gtagtgtatg gggctgaccc aggctcccct gatgtcaatg gccgcacacc    660 

cattgactat gccaggcagg cggggcacca tgaactggca gaaaggctag ttgagtgcca    720 

gtatgagctc actgaccggt tggccttcta cctctgtgga cggaagcctg atcacaagaa    780 

tgggcattac atcataccac agatggctga cagatctcgg caaaagtgca tgtctcagag    840 

tttggatctg tctgaattgg ccaaagctgc caagaagaag ctacaagcac tcagcaaccg    900 

gctctttgag gagctcgcca tggatgtgta tgacgaagtg gaccggagag aaaatgatgc    960 

agtgtggcta gctacccaga atcatagcac cctggtgaca gaacgcagtg ctgtgccctt   1020 

cctgccagtc aaccctgaat actcagccac tcggaatcag ggacgacaga aattggctcg   1080 

ttttaatgcc cgagagtttg ccaccttgat catcgacatc ctcagtgagg ccaaacggag   1140 

gcagcagggc aagagtctga gcagccccac agacaacctc gagctgtctg cacggaacca   1200 

aagtgacctg gacgaccagc acgactacga tagtgtggct tctgacgaag acacagacca   1260 

ggagcccttg cccagtgctg gcgccacgcg gaacaatcgt gccaggagca tggactcctc   1320 

agatctgtcg gatggggctg tgacgctgca ggagtacctg gagctgaaga aggctctggc   1380 

tacctccgag gccaaagtgc agcagctcat gaaagtcaac agcagtctga gtgacgagct   1440 

tcggaagctg cagagggaga tccacaaact gcaggcagag aacctgcagc tccggcagcc   1500 

accaggacca gtgcctgtac cctcacttcc cagtgaacgg gcagaacaca cactcatggg   1560 

ccctggtgga agtacccatc gcagggaccg ccaggccttc tctatgtatg agccaggctc   1620 

cgccctgaag ccctttggag gtgcacctgg ggatgagctc gccacacggc tccagccttt   1680 

ccacagcaca gagctggaag atgatgccat ctattcagta catgtccctg ctggccttta   1740 

ccggatccgg aagggggtgt ctgcctcttc tgtgaccttc actccctcct ccccactgct   1800 

gtcaagctcc caagaaggaa gtcgccatgc gagcaagctt tcacgccacg gcagtggcgc   1860 

agagagtgac tatgagaaca cacagagtgg agagcctctg ctgggacttg aagggaagcg   1920 

attcctagag ctgagcaagg aggatgagct gcacgctgag ctggagagct tagatggaga   1980 

cccagacccc gggctcccca gcacagaaga tgtcatccta aagacagagc aggtcaccaa   2040 

gaacattcag gagctattgc gggcagccca ggagttcaaa catgacagct ttgtgccctg   2100 

ttcagaaaag atccatttgg ctgtgactga gatggcctct ctcttcccaa agaggccagc   2160 

cctggagcct gtgcgcagtt cactgcggct gctcaacgcc agcgcctacc ggctgcagag   2220 

tgaatgccgg aagacagtgc ctccagaacc tggcgcccct gtggacttcc agctactgac   2280 

tcagcaggtg atccagtgcg cctatgacat cgccaaggct gccaagcagc tggtcaccat   2340 

caccacccga gagaagaaac agtgaccact ttccctgccc ttacccacac cctgggacct   2400 

cactggccat gggagctggg ccactccaga cactaaccca cccccaacag agccactggc   2460 

acaagtgccc ttagtgccac cattctcctc tggcatccag gtgccctggt gtccacaccc   2520 

ccttaagccc caaggatggg aatgtgaggt gacaggacca tctgtccccc acattccacg   2580 

cccctccctc tgtacatagc atccctgccc tgctgatacg caggagcctc aaggagtccc   2640 

tcccagctct tcacctgcct tccagggccc cctcagcaaa gggtgggaca gggacactcc   2700 

aagtggggca gccccgccta ctgcacccta tccccatgag ccagttcagc cctggggggt   2760 

cgagcagagg caccccctcc tttgtgcatt atctmtgggt gtytytgccc cgtagccagc   2820 

agcccccccg ctgctctttc atgacaaatg gcccctgcca gagcacaggc cccaacttgt   2880 

gtgccagggt ccccagcagc aaacactgga acgtctttct ctcccctacc ccttaatttt   2940 

aactttgtgg taactgagtg tccctgcgtt cttacaactg agtgtgtgag cggcagtgcc   3000 

gtgccagaga cctggtccac ctggagctct gaggggcagg ggaaccagag tagtgggacc   3060 

agcattggaa gtcagcttcc ctctacccaa cctgggacgg ggactgtcag ggtcaccagc   3120 

tgtggtccag ttgctacccc tacttgtatc cctatcctat aaacttaaaa ggaaaaccac   3180 

ttccctccat ctctccccat cccctgtggg aagtgtgctg accaggcaga ggacag       3236 

 
           
             12  
             136  
             PRT  
             Homo sapiens  
             
               Histone H3.3  
             
           
            12 

Met Ala Arg Thr Lys Gln Thr Ala Arg Lys Ser Thr Gly Gly Lys Ala 
1               5                   10                  15 

Pro Arg Lys Gln Leu Ala Thr Lys Ala Ala Arg Lys Ser Ala Pro Ser 
            20                  25                  30 

Thr Gly Gly Val Lys Lys Pro His Arg Tyr Arg Pro Gly Thr Val Ala 
        35                  40                  45 

Leu Arg Glu Ile Arg Arg Tyr Gln Lys Ser Thr Glu Leu Leu Ile Arg 
    50                  55                  60 

Lys Leu Pro Phe Gln Arg Leu Val Arg Glu Ile Ala Gln Asp Phe Lys 
65                  70                  75                  80 

Thr Asp Leu Arg Phe Gln Ser Ala Ala Ile Gly Ala Leu Gln Glu Ala 
                85                  90                  95 

Ser Glu Ala Tyr Leu Val Gly Leu Phe Glu Asp Thr Asn Leu Cys Ala 
            100                 105                 110 

Ile His Ala Lys Arg Val Thr Ile Met Pro Lys Asp Ile Gln Leu Ala 
        115                 120                 125 

Arg Arg Ile Arg Gly Glu Arg Ala 
    130                 135 

 
           
             13  
             1039  
             DNA  
             Homo sapiens  
             
               Histone H3.3  
             
           
            13 

tgttcgcagc cgccgccgcg ccgccgtcgc tctccaacgc cagcgccgcc tctcgctcgc     60 

gtaagtaagg aggtctctgc gagctccagc cgaagagaag ggggtaccat ggctcgtaca    120 

aagcagactg cccgcaaatc gaccggtggt aaagcaccca ggaagcaact ggctacaaaa    180 

gccgctcgca agagtgcgcc ctctactgga ggggtgaaga aacctcatcg ttacaggcct    240 

ggtactgtgg cgctccgtga aattagacgt tatcagaagt ccactgaact tctgattcgc    300 

aaacttccct tccagcgtct ggtgcgagaa attgctcagg actttaaaac agatctgcgc    360 

ttccagagcg cagctatcgg tgctttgcag gaggcaagtg aggcctatct ggttggcctt    420 

tttgaagaca ccaacctgtg tgctatccat gccaaacgtg taacaattat gccaaaagac    480 

atccagctag cacgccgcat acgtggagaa cgtgcttaag aatccactat gatgggaaac    540 

atttcattct caaaaaaaaa aaaaaaattt ctcttcttcc tgttattggt agttctgaac    600 

gttagatatt ttttttccat ggggtcaaag gtacctaagt atatgattgc gagtggaaaa    660 

ataggggaca gaaatcaggt attggcagtt tttccatttt catttgtgtg tgaattttta    720 

atataaatgc ggagacgtaa agcattaatg caagttaaaa tgtttcagtg aacaagtttc    780 

agcggttcaa ctttataata attataaata aacctgttaa atttttctgg acaatgccag    840 

catttggatt tctttaaaac aagtaaattt cttattgatg gcaactaaat ggtgtttgta    900 

gcatttttat catacagtag attccatcca ttcactatac ttttctaact gagttgtcct    960 

acatgcaagt acatgttttt aatgttgtct gtcttctgtg ctgttcctgt aagtttgcta   1020 

ttaaaataca ttaaactat                                                1039 

 
           
             14  
             520  
             PRT  
             Rattus norvegicus  
             
               Cytosolic 3-hydroxy 3-methylglutaryl coenzyme A 
      synthase  
             
           
            14 

Met Pro Gly Ser Leu Pro Leu Asn Ala Glu Ala Cys Trp Pro Lys Asp 
1               5                   10                  15 

Val Gly Ile Val Ala Leu Glu Ile Tyr Phe Pro Ser Gln Tyr Val Asp 
            20                  25                  30 

Gln Ala Glu Leu Glu Lys Tyr Asp Gly Val Asp Ala Gly Lys Tyr Thr 
        35                  40                  45 

Ile Gly Leu Gly Gln Ala Arg Met Gly Phe Cys Thr Asp Arg Glu Asp 
    50                  55                  60 

Ile Asn Ser Leu Cys Leu Thr Val Val Gln Lys Leu Met Glu Arg Asn 
65                  70                  75                  80 

Ser Leu Ser Tyr Asp Cys Ile Gly Arg Leu Glu Val Gly Thr Glu Thr 
                85                  90                  95 

Ile Ile Asp Lys Ser Lys Ser Val Lys Ser Asn Leu Met Gln Leu Phe 
            100                 105                 110 

Glu Glu Ser Gly Asn Thr Asp Ile Glu Gly Ile Asp Thr Thr Asn Ala 
        115                 120                 125 

Cys Tyr Gly Gly Thr Ala Ala Val Phe Asn Ala Val Asn Trp Ile Glu 
    130                 135                 140 

Ser Ser Ser Trp Asp Gly Arg Tyr Ala Leu Val Val Ala Gly Asp Ile 
145                 150                 155                 160 

Ala Ile Tyr Ala Ser Gly Asn Ala Arg Pro Thr Gly Gly Val Gly Ala 
                165                 170                 175 

Val Ala Leu Leu Ile Gly Pro Asn Ala Pro Val Ile Phe Asp Arg Gly 
            180                 185                 190 

Leu Arg Gly Thr His Met Gln His Ala Tyr Asp Phe Tyr Lys Pro Asp 
        195                 200                 205 

Met Leu Ser Glu Tyr Pro Val Val Asp Gly Lys Leu Ser Ile Gln Cys 
    210                 215                 220 

Tyr Leu Ser Ala Leu Asp Arg Cys Tyr Ser Val Tyr Arg Lys Lys Ile 
225                 230                 235                 240 

Arg Ala Gln Trp Gln Lys Glu Gly Lys Asp Lys Asp Phe Thr Leu Asn 
                245                 250                 255 

Asp Phe Gly Phe Met Ile Phe His Ser Pro Tyr Cys Lys Leu Val Gln 
            260                 265                 270 

Lys Ser Leu Ala Arg Met Phe Leu Asn Asp Phe Leu Asn Asp Gln Asn 
        275                 280                 285 

Arg Asp Lys Asn Ser Ile Tyr Ser Gly Leu Glu Ala Phe Gly Asp Val 
    290                 295                 300 

Lys Leu Glu Asp Thr Tyr Phe Asp Arg Asp Val Glu Lys Ala Phe Met 
305                 310                 315                 320 

Lys Ala Ser Ala Glu Leu Phe Asn Gln Lys Thr Lys Ala Ser Leu Leu 
                325                 330                 335 

Val Ser Asn Gln Asn Gly Asn Met Tyr Thr Ser Ser Val Tyr Gly Ser 
            340                 345                 350 

Leu Ala Ser Val Leu Ala Gln Tyr Ser Pro Gln Gln Leu Ala Gly Lys 
        355                 360                 365 

Arg Ile Gly Val Phe Ser Tyr Gly Ser Gly Leu Ala Ala Thr Leu Tyr 
    370                 375                 380 

Ser Leu Lys Val Thr Gln Asp Ala Thr Pro Gly Ser Ala Leu Asp Lys 
385                 390                 395                 400 

Ile Thr Ala Ser Leu Cys Asp Leu Lys Ser Arg Leu Asp Ser Arg Thr 
                405                 410                 415 

Cys Val Ala Pro Asp Val Phe Ala Glu Asn Met Lys Leu Arg Glu Asp 
            420                 425                 430 

Thr His His Leu Ala Asn Tyr Ile Pro Gln Cys Ser Ile Asp Ser Leu 
        435                 440                 445 

Phe Glu Gly Thr Trp Tyr Leu Val Arg Val Asp Glu Lys His Arg Arg 
    450                 455                 460 

Thr Tyr Ala Arg Arg Pro Ser Thr Asn Asp His Ser Leu Asp Glu Gly 
465                 470                 475                 480 

Val Gly Leu Val His Ser Asn Thr Ala Thr Glu His Ile Pro Ser Pro 
                485                 490                 495 

Ala Lys Lys Val Pro Arg Leu Pro Ala Thr Ser Gly Glu Pro Glu Ser 
            500                 505                 510 

Ala Val Ile Ser Asn Gly Glu His 
        515                 520 

 
           
             15  
             3275  
             DNA  
             Rattus norvegicus  
             
               Cytosolic 3-hydroxy 3-methylglutaryl coenzyme A 
      synthase  
             
           
            15 

cccagggtcc gatcgcgttt ggtgcctgaa ggaggaaccg gtgacagacc tggagactac     60 

agttctctgt ccttcacaca gctctttcac catgcctggg tcacttcctt tgaatgcaga    120 

ggcttgctgg ccaaaagatg tgggaatcgt tgcccttgaa atctactttc cttctcagta    180 

tgttgatcaa gctgagttgg aaaaatacga tggtgtagat gctggaaagt ataccattgg    240 

cctgggccag gccaggatgg gcttctgcac ggatcgcgaa gacatcaact ctctctgcct    300 

gactgtggtt cagaaactga tggagagaaa tagcctttcc tatgactgca ttgggcggct    360 

ggaagtcggg acagagacaa tcatcgacaa atcaaaatcc gtgaagtcta atttgatgca    420 

gctgtttgag gagtctggga atacagatat agaaggaata gatacaacta atgcatgcta    480 

tgggggcaca gccgcagtct tcaatgctgt gaactggatc gaatccagct cttgggatgg    540 

acgatacgct ttggtagttg caggagacat cgctatatat gcctcaggaa acgccaggcc    600 

tacaggtgga gttggagctg tggctctgct aattgggcca aatgctcctg taatttttga    660 

ccgagggctt cgtgggacac acatgcagca tgcctacgac ttttacaagc ctgacatgct    720 

ctctgaatac cctgtggtag atggaaaact ctccatacag tgctacctca gcgcattgga    780 

ccgctgctat tctgtctacc gcaaaaagat ccgggcccag tggcagaaag agggaaagga    840 

taaagatttt accctgaatg attttggctt catgatcttt cactcgccat actgtaaact    900 

ggtgcagaaa tctctagcta ggatgttcct gaatgacttt cttaacgatc aaaacagaga    960 

caaaaacagt atttacagtg ggctggaagc ctttggggat gtgaaattag aagatactta   1020 

cttcgacaga gatgtggaaa aggcatttat gaaggctagt gctgagctat tcaaccagaa   1080 

aacaaaggca tctttgcttg tatcgaatca aaatggaaac atgtacacat cctctgtata   1140 

cggttccctt gcttctgttc tggcacagta ctcacctcaa cagttggccg ggaagaggat   1200 

tggagtgttc tcttacggtt ctggcttggc tgccacactc tactccctta aagtcacaca   1260 

agatgccaca ccaggatctg ctcttgacaa aataacagca agtttatgtg accttaagtc   1320 

aaggcttgac tcaagaacgt gtgtggcacc agacgtcttt gctgaaaaca tgaagctcag   1380 

agaggacaca catcacttag ccaactatat tccccagtgt tcaatagatt cactcttcga   1440 

aggaacatgg tatctagtca gagtggatga aaagcacaga agaacttacg cccggcgtcc   1500 

ctccacaaat gaccacagtt tggatgaagg agtgggactt gtgcattcaa acacagctac   1560 

agagcatatt ccaagcccgg ctaagaaagt gccaagactt cctgcgacct cgggcgaacc   1620 

tgagtcggct gtcatcagta acggggagca ctgagagccg tggccttcac agaggctcgg   1680 

ggctggatgg ggtatgggaa acgttagagg aatggatgtc ttgggacaat tttacagatt   1740 

acgtgttgct taaaaatgta atgtaactga cacagagccc agaaaactgt tgtgtttttg   1800 

gagaagtctc gctgagctcc taacacactt cctgctgtgg gctggccaat ggtgaatgta   1860 

ctgcgatggt gttaagggct ctgcagaacg tcatacctcg ctgcatgttt acacgcatgc   1920 

gggttaggct tcaaactcgg tctgaactga gtgcttctga ctgcaaaggc agaggtactg   1980 

ctgtccagtt taaaaaattg tttttttttt ttttaatgtg taagaatttt tatacttaaa   2040 

taaaaaaaag tacctgtagc ttttggggga aaaaaaaaaa cctttttcta ggttggggat   2100 

tgtggaattt aaatgttaca cataaactct gcttaatggc aaggcaaaca tttatctttt   2160 

tcgaagattt ataaatcctg aagagaaaaa aagagggtat ggttctagga tctggatgaa   2220 

ccatcagtga gaaaggttag tcataatcaa gtgagcagaa ggatgctggc gttgagcagg   2280 

cctctgtcac agcaaccagg gctctgtggg cacagctgag ggaactttct ggccaggtgc   2340 

ccgtgactgc tgctcagctg cactgagatg cagtggagct gctgcacgga agcttgctgt   2400 

ggtgctgaac gccttacctg cggataaagt gtaaagtagg agggatgggc agggcactat   2460 

taggttacag tgttacagac ccaggttata gacttgacag ctcaaactca ccagacacct   2520 

ttttcccttt gtggtttgtg tattttttgt gttttgtttg ttttttttta tattgttcaa   2580 

tttaaaaaat ttagaaaatt ttaaccttac gttttcacat agtgtgatta gccaaaagga   2640 

atttcacttc aagatctaga aatagaattc ataacatttt ttcctaaact ttgactttta   2700 

aaacaacgaa aattaccaca tgagatgaac aagaaaattc attagaaagt tctctgggtg   2760 

atttttggtg ctgaactgac atgagcctca tagactgtaa aacagaggta gttgaaacta   2820 

atgtacagaa ctacattttt taattttatt tgcatttaat tctgtgaagt ttcagttatc   2880 

taaaataaac acataaacgt gtaatgtttc agattgcaag gtgagatgta atgtagcatt   2940 

tgtaagatat tcttgtcaat attaactggt aggattttga tttgtacagt tttaattggt   3000 

taaaatgatc tcattttaac atccactgct atagatgaat gatgtaagct cagatttaat   3060 

gaatggtggg gaaatggtgc atgtaatttt ttcgcaagta tcgagagttc tgtatgtttt   3120 

gaaaagaata atttaacgtt tgggttgcca ggaagggggc tttcccagag tccattgcca   3180 

ggcgttgggc aagcctcgca atgttggcac ggagcgttaa ccacacctta ctaatagcaa   3240 

ggggaataac tttgaaataa agttttagac aaata                              3275 

 
           
             16  
             353  
             PRT  
             Rattus norvegicus  
             
               farnesyl pyrophosphate synthetase  
             
           
            16 

Met Asn Gly Asp Gln Lys Leu Asp Val His Asn Gln Glu Lys Gln Asn 
1               5                   10                  15 

Phe Ile Gln His Phe Ser Gln Ile Val Lys Val Leu Thr Glu Asp Glu 
            20                  25                  30 

Leu Gly His Pro Glu Lys Gly Asp Ala Ile Thr Arg Ile Lys Glu Val 
        35                  40                  45 

Leu Glu Tyr Asn Thr Val Gly Gly Lys Tyr Asn Arg Gly Leu Thr Val 
    50                  55                  60 

Val Gln Thr Phe Gln Glu Leu Val Glu Pro Arg Lys Gln Asp Ala Glu 
65                  70                  75                  80 

Ser Leu Gln Arg Ala Leu Thr Val Gly Trp Cys Val Glu Leu Leu Gln 
                85                  90                  95 

Ala Phe Phe Leu Val Leu Asp Asp Ile Met Asp Ser Ser His Thr Arg 
            100                 105                 110 

Arg Gly Gln Ile Cys Trp Tyr Gln Lys Pro Gly Ile Gly Leu Asp Ala 
        115                 120                 125 

Ile Asn Asp Ala Leu Leu Leu Glu Ala Ala Ile Tyr Arg Leu Leu Lys 
    130                 135                 140 

Phe Tyr Cys Arg Glu Gln Pro Tyr Tyr Leu Asn Leu Leu Glu Leu Phe 
145                 150                 155                 160 

Leu Gln Ser Ser Tyr Gln Thr Glu Ile Gly Gln Thr Leu Asp Leu Ile 
                165                 170                 175 

Thr Ala Pro Gln Gly Gln Val Asp Leu Gly Arg Tyr Thr Glu Lys Arg 
            180                 185                 190 

Tyr Lys Ser Ile Val Lys Tyr Lys Thr Ala Phe Tyr Ser Phe Tyr Leu 
        195                 200                 205 

Pro Ile Ala Ala Ala Met Tyr Met Ala Gly Ile Asp Gly Glu Lys Glu 
    210                 215                 220 

His Ala Asn Ala Leu Lys Ile Leu Leu Glu Met Gly Glu Phe Phe Gln 
225                 230                 235                 240 

Ile Gln Asp Asp Tyr Leu Asp Leu Phe Gly Asp Pro Ser Val Thr Gly 
                245                 250                 255 

Lys Val Gly Thr Asp Ile Gln Asp Asn Lys Cys Ser Trp Leu Val Val 
            260                 265                 270 

Gln Cys Leu Leu Arg Ala Thr Pro Gln Gln Arg Gln Ile Leu Glu Glu 
        275                 280                 285 

Asn Tyr Gly Gln Lys Asp Pro Glu Lys Val Ala Arg Val Lys Ala Leu 
    290                 295                 300 

Tyr Glu Glu Leu Asp Leu Arg Ser Val Phe Phe Lys Tyr Glu Glu Asp 
305                 310                 315                 320 

Ser Tyr Asn Arg Leu Lys Ser Leu Ile Glu Gln Cys Ser Ala Pro Leu 
                325                 330                 335 

Pro Pro Ser Ile Phe Leu Glu Leu Ala Asn Lys Ile Tyr Lys Arg Arg 
            340                 345                 350 

Lys 

 
           
             17  
             1271  
             DNA  
             Rattus norvegicus  
             
               farnesyl pyrophosphate synthetase  
             
           
            17 

ttatatttgg gttctgccta ctgagccggg agtctgggaa ctacaactcc cagagtgctg     60 

agcggatgca cgctctgctt ttaggtgtaa gccgcaaaca tcttggaccc cgggagaatc    120 

cgcgttgaag cacagagcat ttagctcctc tgtcagaatg aatggggacc agaaactgga    180 

tgttcataac caagaaaagc agaatttcat ccagcacttc tcccagattg tcaaggtgct    240 

gactgaggat gaactgggac acccagagaa gggagatgct attacccgga tcaaagaggt    300 

cctggagtac aacactgtag gaggcaagta caatcggggt ctgacggtgg tacagacctt    360 

ccaggaactg gtggaaccaa ggaaacagga tgctgagagc ctacagcggg ccctgacggt    420 

gggctggtgt gtagaactgc tccaggcttt cttcctcgtg ttagatgaca tcatggactc    480 

ttcccacact cgccgggggc agatctgctg gtatcagaag ccgggcatag gcttggatgc    540 

catcaacgat gctctgcttc tggaagccgc tatctaccgc ctgcttaagt tctactgcag    600 

ggagcagccc tactacctca acctgctgga gctctttcta cagagttcct atcagactga    660 

gatcgggcag actctcgacc tcatcacagc accccagggc caagtggatc ttggtagata    720 

cactgaaaag aggtacaaat ctatcgtcaa gtacaagaca gctttctact ctttctacct    780 

gcctatcgcg gctgccatgt acatggctgg aattgatggg gagaaggaac acgctaatgc    840 

cctgaagatc ctgctggaga tgggcgagtt cttccagatc caggacgact accttgatct    900 

ctttggagac cccagtgtga ccggaaaggt cggcactgac atccaggaca acaaatgcag    960 

ctggctggtg gttcagtgtc tgctacgagc cactcctcag cagcgccaga tcttagagga   1020 

gaattatggg cagaaggacc cagaaaaagt ggcgcgggtg aaagcactgt acgaggagct   1080 

ggatctgcgg agtgtgttct tcaagtacga ggaagacagt tacaaccgcc tcaagagtct   1140 

catagagcag tgctccgcgc ccctgccccc atccatcttc ctggaactag caaacaagat   1200 

ctacaagcgg agaaagtaac ctcgaattgt agaggctgcg agggaggggt ctcaataaat   1260 

tattgttcaa c                                                        1271 

 
           
             18  
             152  
             PRT  
             Rattus norvegicus  
             
               Bendless protein  
             
           
            18 

Met Ala Gly Leu Pro Arg Arg Ile Ile Lys Glu Thr Gln Arg Leu Leu 
1               5                   10                  15 

Ala Glu Pro Val Pro Gly Ile Lys Ala Glu Pro Asp Glu Ser Asn Ala 
            20                  25                  30 

Arg Tyr Phe His Val Val Ile Ala Gly Pro Gln Asp Ser Pro Phe Glu 
        35                  40                  45 

Gly Gly Thr Phe Lys Leu Glu Leu Phe Leu Pro Glu Glu Tyr Pro Met 
    50                  55                  60 

Ala Ala Pro Lys Val Arg Phe Met Thr Lys Ile Tyr His Pro Asn Val 
65                  70                  75                  80 

Asp Lys Leu Gly Arg Ile Cys Leu Asp Ile Leu Lys Asp Lys Trp Ser 
                85                  90                  95 

Pro Ala Leu Gln Ile Arg Thr Val Leu Leu Ser Ile Gln Ala Leu Leu 
            100                 105                 110 

Ser Ala Pro Asn Pro Asp Asp Pro Leu Ala Asn Asp Val Ala Glu Gln 
        115                 120                 125 

Trp Lys Ser Asn Glu Ala Gln Ala Ile Glu Thr Ala Arg Ala Trp Thr 
    130                 135                 140 

Arg Leu Tyr Ala Met Asn Asn Ile 
145                 150 

 
           
             19  
             459  
             DNA  
             Rattus norvegicus  
             
               Bendless protein  
             
           
            19 

atggccgggc tgccccgcag gatcatcaag gaaacccagc gtttgctggc agaaccagtt     60 

cctggcatta aagcagaacc agatgagagc aacgcccgtt attttcatgt ggtcattgct    120 

ggtccccagg attccccctt tgagggaggg acttttaaac ttgaactatt ccttccagaa    180 

gaatacccaa tggcagcacc taaagtacgt ttcatgacca aaatttatca tcctaatgta    240 

gacaagttgg gaagaatatg tttagatatt ttgaaagata agtggtcccc agcactgcag    300 

atccgaacag ttctgctatc aatccaggct ttgctaagtg ctcctaatcc agatgatcca    360 

ttagcaaatg atgtagctga gcagtggaag agcaacgaag cccaagccat agaaacagca    420 

agagcatgga ctaggctata tgccatgaat aatatttaa                           459 

 
           
             20  
             2505  
             PRT  
             Rattus norvegicus  
             
               Fatty acid synthase  
             
           
            20 

Met Glu Glu Val Val Ile Ala Gly Met Ser Gly Lys Leu Pro Glu Ser 
1               5                   10                  15 

Glu Asn Leu Gln Glu Phe Trp Ala Asn Leu Ile Gly Gly Val Asp Met 
            20                  25                  30 

Val Thr Asp Asp Asp Arg Arg Trp Lys Ala Gly Leu Tyr Gly Leu Pro 
        35                  40                  45 

Lys Arg Ser Gly Lys Leu Lys Asp Leu Ser Lys Phe Asp Ala Ser Phe 
    50                  55                  60 

Phe Gly Val His Pro Lys Gln Ala His Thr Met Asp Pro Gln Leu Arg 
65                  70                  75                  80 

Leu Leu Leu Glu Val Ser Tyr Glu Ala Ile Val Asp Gly Gly Ile Asn 
                85                  90                  95 

Pro Ala Ser Leu Arg Gly Thr Asn Thr Gly Val Trp Val Gly Val Ser 
            100                 105                 110 

Gly Ser Glu Ala Ser Glu Ala Leu Ser Arg Asp Pro Glu Thr Leu Leu 
        115                 120                 125 

Gly Tyr Ser Met Val Gly Cys Gln Arg Ala Met Met Ala Asn Arg Leu 
    130                 135                 140 

Ser Phe Phe Phe Asp Phe Lys Gly Pro Ser Ile Ala Leu Asp Thr Ala 
145                 150                 155                 160 

Cys Ser Ser Ser Leu Leu Ala Leu Gln Asn Ala Tyr Gln Ala Ile Arg 
                165                 170                 175 

Ser Gly Glu Cys Pro Ala Ala Ile Val Gly Gly Ile Asn Leu Leu Leu 
            180                 185                 190 

Lys Pro Asn Thr Ser Val Gln Phe Met Lys Leu Gly Met Leu Ser Pro 
        195                 200                 205 

Asp Gly Thr Cys Arg Ser Phe Asp Asp Ser Gly Asn Gly Tyr Cys Arg 
    210                 215                 220 

Ala Glu Ala Val Val Ala Val Leu Leu Thr Lys Lys Ser Leu Ala Arg 
225                 230                 235                 240 

Arg Val Tyr Ala Thr Ile Leu Asn Ala Gly Thr Asn Thr Asp Gly Cys 
                245                 250                 255 

Lys Glu Gln Gly Val Thr Phe Pro Ser Gly Glu Ala Gln Glu Gln Leu 
            260                 265                 270 

Ile Arg Ser Leu Tyr Gln Pro Gly Gly Val Ala Pro Glu Ser Leu Glu 
        275                 280                 285 

Tyr Ile Glu Ala His Gly Thr Gly Thr Lys Val Gly Asp Pro Gln Glu 
    290                 295                 300 

Leu Asn Gly Ile Thr Arg Ser Leu Cys Ala Phe Arg Gln Ser Pro Leu 
305                 310                 315                 320 

Leu Ile Gly Ser Thr Lys Ser Asn Met Gly His Pro Glu Pro Ala Ser 
                325                 330                 335 

Gly Leu Ala Ala Leu Thr Lys Val Leu Leu Ser Leu Glu Asn Gly Val 
            340                 345                 350 

Trp Ala Pro Asn Leu His Phe His Asn Pro Asn Pro Glu Ile Pro Ala 
        355                 360                 365 

Leu Leu Asp Gly Arg Leu Gln Val Val Asp Arg Pro Leu Pro Val Arg 
    370                 375                 380 

Gly Gly Ile Val Gly Ile Asn Ser Phe Gly Phe Gly Gly Ala Asn Val 
385                 390                 395                 400 

His Val Ile Leu Gln Pro Asn Thr Gln Gln Ala Pro Ala Pro Ala Pro 
                405                 410                 415 

His Ala Ala Leu Pro His Leu Leu His Ala Ser Gly Arg Thr Met Glu 
            420                 425                 430 

Ala Val Gln Gly Leu Leu Glu Gln Gly Arg Gln His Ser Gln Asp Leu 
        435                 440                 445 

Ala Phe Val Ser Met Leu Asn Asp Ile Ala Ala Thr Pro Thr Ala Ala 
    450                 455                 460 

Met Pro Phe Arg Gly Tyr Thr Val Leu Gly Val Glu Gly His Val Gln 
465                 470                 475                 480 

Glu Val Gln Gln Val Pro Ala Ser Gln Arg Pro Leu Trp Phe Ile Cys 
                485                 490                 495 

Ser Gly Met Gly Thr Gln Trp Arg Gly Met Gly Leu Ser Leu Met Arg 
            500                 505                 510 

Leu Asp Ser Phe Arg Glu Ser Ile Leu Arg Ser Asp Glu Ala Leu Lys 
        515                 520                 525 

Pro Leu Gly Val Lys Val Ser Asp Leu Leu Leu Ser Thr Asp Glu His 
    530                 535                 540 

Thr Phe Asp Asp Ile Val His Ser Phe Val Ser Leu Thr Ala Ile Gln 
545                 550                 555                 560 

Ile Ala Leu Ile Asp Leu Leu Thr Ser Met Gly Leu Lys Pro Asp Gly 
                565                 570                 575 

Ile Ile Gly His Ser Leu Gly Glu Val Ala Cys Gly Tyr Ala Asp Gly 
            580                 585                 590 

Cys Leu Ser Gln Arg Glu Ala Val Leu Ala Ala Tyr Trp Arg Gly Gln 
        595                 600                 605 

Cys Ile Lys Asp Ala Asn Leu Pro Ala Gly Ser Met Ala Ala Val Gly 
    610                 615                 620 

Leu Ser Trp Glu Glu Cys Lys Gln Arg Cys Pro Pro Gly Val Val Pro 
625                 630                 635                 640 

Ala Cys His Asn Ser Glu Asp Thr Val Thr Ile Ser Gly Pro Gln Ala 
                645                 650                 655 

Ala Val Asn Glu Phe Val Glu Gln Leu Lys Gln Glu Gly Val Phe Ala 
            660                 665                 670 

Lys Glu Val Arg Thr Gly Gly Leu Ala Phe His Ser Tyr Phe Met Glu 
        675                 680                 685 

Gly Ile Ala Pro Thr Leu Leu Gln Ala Leu Lys Lys Val Ile Arg Glu 
    690                 695                 700 

Pro Arg Pro Arg Ser Ala Arg Trp Leu Ser Thr Ser Ile Pro Glu Ala 
705                 710                 715                 720 

Gln Trp Gln Ser Ser Leu Ala Arg Thr Ser Ser Ala Glu Tyr Asn Val 
                725                 730                 735 

Asn Asn Leu Val Ser Pro Val Leu Phe Gln Glu Ala Leu Trp His Val 
            740                 745                 750 

Pro Glu His Ala Val Val Leu Glu Ile Ala Pro His Ala Leu Leu Gln 
        755                 760                 765 

Ala Val Leu Lys Arg Gly Val Lys Pro Ser Cys Thr Ile Ile Pro Leu 
    770                 775                 780 

Met Lys Arg Asp His Lys Asp Asn Leu Glu Phe Phe Leu Thr Asn Leu 
785                 790                 795                 800 

Gly Lys Val His Leu Thr Gly Ile Asp Ile Asn Pro Asn Ala Leu Phe 
                805                 810                 815 

Pro Pro Val Glu Phe Pro Val Pro Arg Gly Thr Pro Leu Ile Ser Pro 
            820                 825                 830 

His Ile Lys Trp Asp His Ser Gln Thr Trp Asp Ile Pro Val Ala Glu 
        835                 840                 845 

Asp Phe Pro Asn Gly Ser Ser Ser Ser Ser Ala Thr Val Tyr Asn Ile 
    850                 855                 860 

Asp Ala Ser Ser Glu Ser Ser Asp His Tyr Leu Val Asp His Cys Ile 
865                 870                 875                 880 

Asp Gly Arg Val Leu Phe Pro Gly Thr Gly Tyr Leu Tyr Leu Val Trp 
                885                 890                 895 

Lys Thr Leu Ala Arg Ser Leu Ser Leu Ser Leu Glu Glu Thr Pro Val 
            900                 905                 910 

Val Phe Glu Asn Val Thr Phe His Gln Ala Thr Ile Leu Pro Arg Thr 
        915                 920                 925 

Gly Thr Val Pro Leu Glu Val Arg Leu Leu Glu Ala Ser His Ala Phe 
    930                 935                 940 

Glu Val Ser Asp Ser Gly Asn Leu Ile Val Ser Gly Lys Val Tyr Gln 
945                 950                 955                 960 

Trp Glu Asp Pro Asp Ser Lys Leu Phe Asp His Pro Glu Val Pro Ile 
                965                 970                 975 

Pro Ala Glu Ser Glu Ser Val Ser Arg Leu Thr Gln Gly Glu Val Tyr 
            980                 985                 990 

Lys Glu Leu Arg Leu Arg Gly Tyr Asp Tyr Gly Pro His Phe Gln Gly 
        995                 1000                1005 

Val Tyr Glu Ala Thr Leu Glu Gly Glu Gln Gly Lys Leu Leu Trp Lys 
    1010                1015                1020 

Asp Asn Trp Val Thr Phe Met Asp Thr Met Leu Gln Ile Ser Ile Leu 
 1025               1030                1035                1040 

Gly Phe Ser Lys Gln Ser Leu Gln Leu Pro Thr Arg Val Thr Ala Ile 
                1045                1050                1055 

Tyr Ile Asp Pro Ala Thr His Leu Gln Lys Val Tyr Met Leu Glu Gly 
            1060                1065                1070 

Asp Thr Gln Val Ala Asp Val Thr Thr Ser Arg Cys Leu Gly Val Thr 
        1075                1080                1085 

Val Ser Gly Gly Val Tyr Ile Ser Arg Leu Gln Thr Thr Ala Thr Ser 
    1090                1095                1100 

Arg Arg Gln Gln Glu Gln Leu Val Pro Thr Leu Glu Lys Phe Val Phe 
1105                1110                1115                1120 

Thr Pro His Val Glu Pro Glu Cys Leu Ser Glu Ser Ala Ile Leu Gln 
                1125                1130                1135 

Lys Glu Leu Gln Leu Cys Lys Gly Leu Ala Lys Ala Leu Gln Thr Lys 
            1140                1145                1150 

Ala Thr Gln Gln Gly Leu Lys Met Thr Val Pro Gly Leu Glu Asp Leu 
        1155                1160                1165 

Pro Gln His Gly Leu Pro Arg Leu Leu Ala Ala Ala Cys Gln Leu Gln 
    1170                1175                1180 

Leu Asn Gly Asn Leu Gln Leu Glu Leu Gly Glu Val Leu Ala Arg Glu 
1185                1190                1195                1200 

Arg Leu Leu Leu Pro Glu Asp Pro Leu Ile Ser Gly Leu Leu Asn Ser 
                1205                1210                1215 

Gln Ala Leu Lys Ala Cys Ile Asp Thr Ala Leu Glu Asn Leu Ser Thr 
            1220                1225                1230 

Leu Lys Met Lys Val Val Glu Val Leu Ala Gly Glu Gly His Leu Tyr 
        1235                1240                1245 

Ser His Ile Ser Ala Leu Leu Asn Thr Gln Pro Met Leu Gln Leu Glu 
    1250                1255                1260 

Tyr Thr Ala Thr Asp Arg His Pro Gln Ala Leu Lys Asp Val Gln Thr 
1265                1270                1275                1280 

Lys Leu Gln Gln His Asp Val Ala Gln Gly Gln Trp Asp Pro Ser Gly 
                1285                1290                1295 

Pro Ala Pro Thr Asn Leu Gly Ala Leu Asp Leu Val Val Cys Asn Cys 
            1300                1305                1310 

Ala Leu Ala Thr Leu Gly Asp Pro Ala Leu Ala Leu Asp Asn Met Val 
        1315                1320                1325 

Ala Ala Leu Lys Asp Gly Gly Phe Leu Leu Met His Thr Val Leu Lys 
    1330                1335                1340 

Gly His Ala Leu Gly Glu Thr Leu Ala Cys Leu Pro Ser Glu Val Gln 
1345                1350                1355                1360 

Pro Gly Pro Ser Phe Leu Ser Gln Glu Glu Trp Glu Ser Leu Phe Ser 
                1365                1370                1375 

Arg Lys Ala Leu His Leu Val Gly Leu Lys Lys Ser Phe Tyr Gly Thr 
            1380                1385                1390 

Ala Leu Phe Leu Cys Arg Arg Leu Ser Pro Gln Asp Lys Pro Ile Phe 
        1395                1400                1405 

Leu Pro Val Glu Asp Thr Ser Phe Gln Trp Val Asp Ser Leu Lys Ser 
    1410                1415                1420 

Ile Leu Ala Thr Ser Ser Ser Gln Pro Val Trp Leu Thr Ala Met Asn 
1425                1430                1435                1440 

Cys Pro Thr Ser Gly Val Val Gly Leu Val Asn Cys Leu Arg Lys Glu 
                1445                1450                1455 

Pro Gly Gly His Arg Ile Arg Cys Ile Leu Leu Ser Asn Leu Ser Ser 
            1460                1465                1470 

Thr Ser His Val Pro Lys Leu Asp Pro Gly Ser Ser Glu Leu Gln Lys 
        1475                1480                1485 

Val Leu Glu Ser Asp Leu Val Met Asn Val Tyr Arg Asp Gly Ala Trp 
    1490                1495                1500 

Gly Ala Phe Arg His Phe Gln Leu Glu Gln Asp Lys Pro Glu Glu Gln 
1505                1510                1515                1520 

Thr Ala His Ala Phe Val Asn Val Leu Thr Arg Gly Asp Leu Ala Ser 
                1525                1530                1535 

Ile Arg Trp Val Ser Ser Pro Leu Lys His Met Gln Pro Pro Ser Ser 
            1540                1545                1550 

Ser Gly Ala Gln Leu Cys Thr Val Tyr Tyr Ala Ser Leu Asn Phe Arg 
        1555                1560                1565 

Asp Ile Met Leu Ala Thr Gly Lys Leu Ser Pro Asp Ala Ile Pro Gly 
    1570                1575                1580 

Lys Trp Ala Ser Arg Asp Cys Met Leu Gly Met Glu Phe Ser Gly Arg 
1585                1590                1595                1600 

Asp Lys Cys Gly Arg Arg Val Met Gly Leu Val Pro Ala Glu Gly Leu 
                1605                1610                1615 

Ala Thr Ser Val Leu Leu Ser Pro Asp Phe Leu Trp Asp Val Pro Ser 
            1620                1625                1630 

Ser Trp Thr Leu Glu Glu Ala Ala Ser Val Pro Val Val Tyr Thr Thr 
        1635                1640                1645 

Ala Tyr Tyr Ser Leu Val Val Arg Gly Arg Ile Gln His Gly Glu Thr 
    1650                1655                1660 

Val Leu Ile His Ser Gly Ser Gly Gly Val Gly Gln Ala Ala Ile Ser 
1665                1670                1675                1680 

Ile Ala Leu Ser Leu Gly Cys Arg Val Phe Thr Thr Val Gly Ser Ala 
                1685                1690                1695 

Glu Lys Arg Ala Tyr Leu Gln Ala Arg Phe Pro Gln Leu Asp Asp Thr 
            1700                1705                1710 

Ser Phe Ala Asn Ser Arg Asp Thr Ser Phe Glu Gln His Val Leu Leu 
        1715                1720                1725 

His Thr Gly Gly Lys Gly Val Asp Leu Val Leu Asn Ser Leu Ala Glu 
    1730                1735                1740 

Glu Lys Leu Gln Ala Ser Val Arg Cys Leu Ala Gln His Gly Arg Phe 
1745                1750                1755                1760 

Leu Glu Ile Gly Lys Phe Asp Leu Ser Asn Asn His Pro Leu Gly Met 
                1765                1770                1775 

Ala Ile Phe Leu Lys Asn Val Thr Phe His Gly Ile Leu Leu Asp Ala 
            1780                1785                1790 

Leu Phe Glu Gly Ala Asn Asp Ser Trp Arg Glu Val Ala Glu Leu Leu 
        1795                1800                1805 

Lys Ala Gly Ile Arg Asp Gly Val Val Lys Pro Leu Lys Cys Thr Val 
    1810                1815                1820 

Phe Pro Lys Ala Gln Val Glu Asp Ala Phe Arg Tyr Met Ala Gln Gly 
1825                1830                1835                1840 

Lys His Ile Gly Lys Val Leu Val Gln Val Arg Glu Glu Glu Pro Glu 
                1845                1850                1855 

Ala Met Leu Pro Gly Ala Gln Pro Thr Leu Ile Ser Ala Ile Ser Lys 
            1860                1865                1870 

Thr Phe Cys Pro Glu His Lys Ser Tyr Ile Ile Thr Gly Gly Leu Gly 
        1875                1880                1885 

Gly Phe Gly Leu Glu Leu Ala Arg Trp Leu Val Leu Arg Gly Ala Gln 
    1890                1895                1900 

Arg Leu Val Leu Thr Ser Arg Ser Gly Ile Arg Thr Gly Tyr Gln Ala 
1905                1910                1915                1920 

Lys His Val Arg Glu Trp Arg Arg Gln Gly Ile His Val Leu Val Ser 
                1925                1930                1935 

Thr Ser Asn Val Ser Ser Leu Glu Gly Ala Arg Ala Leu Ile Ala Glu 
            1940                1945                1950 

Ala Thr Lys Leu Gly Pro Val Gly Gly Val Phe Asn Leu Ala Met Val 
        1955                1960                1965 

Leu Arg Asp Ala Met Leu Glu Asn Gln Thr Pro Glu Leu Phe Gln Asp 
    1970                1975                1980 

Val Asn Lys Pro Lys Tyr Asn Gly Thr Leu Asn Leu Asp Arg Ala Thr 
1985                1990                1995                2000 

Arg Glu Ala Cys Pro Glu Leu Asp Tyr Phe Val Ala Phe Ser Ser Val 
                2005                2010                2015 

Ser Cys Gly Arg Gly Asn Ala Gly Gln Ser Asn Tyr Gly Phe Ala Asn 
            2020                2025                2030 

Ser Thr Met Glu Arg Ile Cys Glu Gln Arg Arg His Asp Gly Leu Pro 
        2035                2040                2045 

Gly Leu Ala Val Gln Trp Gly Ala Ile Gly Asp Val Gly Ile Ile Leu 
    2050                2055                2060 

Glu Ala Met Gly Thr Asn Asp Thr Val Val Gly Gly Thr Leu Pro Gln 
2065                2070                2075                2080 

Arg Ile Ser Ser Cys Met Glu Val Leu Asp Leu Phe Leu Asn Gln Pro 
                2085                2090                2095 

His Ala Val Leu Ser Ser Phe Val Leu Ala Glu Lys Lys Ala Val Ala 
            2100                2105                2110 

His Gly Asp Gly Glu Ala Gln Arg Asp Leu Val Lys Ala Val Ala His 
        2115                2120                2125 

Ile Leu Gly Ile Arg Asp Leu Ala Gly Ile Asn Leu Asp Ser Ser Leu 
    2130                2135                2140 

Ala Asp Leu Gly Leu Asp Ser Leu Met Gly Val Glu Val Arg Gln Ile 
2145                2150                2155                2160 

Leu Glu Arg Glu His Asp Leu Val Leu Pro Ile Arg Glu Val Arg Gln 
                2165                2170                2175 

Leu Thr Leu Arg Lys Leu Gln Glu Met Ser Ser Lys Ala Gly Ser Asp 
            2180                2185                2190 

Thr Glu Leu Ala Ala Pro Lys Ser Lys Asn Asp Thr Ser Leu Lys Gln 
        2195                2200                2205 

Ala Gln Leu Asn Leu Ser Ile Leu Leu Val Asn Pro Glu Gly Pro Thr 
    2210                2215                2220 

Leu Thr Arg Leu Asn Ser Val Gln Ser Ser Glu Arg Pro Leu Phe Leu 
2225                2230                2235                2240 

Val His Pro Ile Glu Gly Ser Ile Thr Val Phe His Ser Leu Ala Ala 
                2245                2250                2255 

Lys Leu Ser Val Pro Thr Tyr Gly Leu Gln Cys Thr Gln Ala Ala Pro 
            2260                2265                2270 

Leu Asp Ser Ile Pro Asn Leu Ala Ala Tyr Tyr Ile Asp Cys Ile Lys 
        2275                2280                2285 

Gln Val Gln Pro Glu Gly Pro Tyr Arg Val Ala Gly Tyr Ser Phe Gly 
    2290                2295                2300 

Ala Cys Val Ala Phe Glu Met Cys Ser Gln Leu Gln Ala Gln Gln Gly 
2305                2310                2315                2320 

Pro Ala Pro Ala His Asn Asn Leu Phe Leu Phe Asp Gly Ser His Thr 
                2325                2330                2335 

Tyr Val Leu Ala Tyr Thr Gln Ser Tyr Arg Ala Lys Leu Thr Pro Gly 
            2340                2345                2350 

Cys Glu Ala Glu Ala Glu Ala Glu Ala Ile Cys Phe Phe Ile Lys Gln 
        2355                2360                2365 

Phe Val Asp Ala Glu His Ser Lys Val Leu Glu Ala Leu Leu Pro Leu 
    2370                2375                2380 

Lys Ser Leu Glu Asp Arg Val Ala Ala Ala Val Asp Leu Ile Thr Arg 
2385                2390                2395                2400 

Ser His Gln Ser Leu Asp Arg Arg Asp Leu Ser Phe Ala Ala Val Ser 
                2405                2410                2415 

Phe Tyr Tyr Lys Leu Arg Ala Ala Asp Gln Tyr Lys Pro Lys Ala Lys 
            2420                2425                2430 

Tyr His Gly Asn Val Ile Leu Leu Arg Ala Lys Thr Gly Gly Thr Tyr 
        2435                2440                2445 

Gly Glu Asp Leu Gly Ala Asp Tyr Asn Leu Ser Gln Val Cys Asp Gly 
    2450                2455                2460 

Lys Val Ser Val His Ile Ile Glu Gly Asp His Arg Thr Leu Leu Glu 
2465                2470                2475                2480 

Gly Arg Gly Leu Glu Ser Ile Ile Asn Ile Ile His Ser Ser Leu Ala 
                2485                2490                2495 

Glu Pro Arg Val Ser Val Arg Glu Gly 
            2500                2505 

 
           
             21  
             9143  
             DNA  
             Rattus norvegicus  
             
               Fatty acid synthase  
             
           
            21 

agagagacgg cagcagcgtc ccgtccagtt cgcctgccgc gctcctcgct tgtcgtctgc     60 

ctccagatcc cagacagaga agagccatgg aggaggtggt gatagccggt atgtccggga    120 

aattgcccga gtcagagaac ctgcaggagt tctgggccaa cctcattggc ggtgtggaca    180 

tggtcacaga cgatgacagg aggtggaagg ctgggctcta tgggttgcct aagcggtctg    240 

gaaagctgaa ggatctgtcc aagttcgacg cctccttttt tggggtccac cccaagcagg    300 

cacacacaat ggacccgcag ctccggctgc tgctggaagt cagctatgaa gctattgtgg    360 

acggaggtat caacccggcc tcactccgag gaacaaacac tggtgtctgg gtgggtgtga    420 

gtggttccga ggcgtcggag gccctgagca gagatcctga gactcttctg ggctacagca    480 

tggtgggctg ccagagagca atgatggcca accggctctc tttcttcttc gacttcaaag    540 

gacccagcat tgccctggac acagcctgct cctctagcct actggcacta cagaatgcct    600 

atcaggctat ccgcagtggg gagtgccctg ctgccattgt gggcgggatc aacctgctgc    660 

taaagcctaa cacctctgtg cagttcatga agctaggcat gctcagcccc gatggcacct    720 

gcagatcctt tgatgattca gggaacgggt attgccgtgc tgaggctgtc gtggcagttc    780 

tgctgactaa gaagtccttg gctcggcgag tctatgccac tattctgaat gccgggacga    840 

acacagatgg ctgcaaggag caaggcgtga cattcccctc tggagaagcc caggaacaac    900 

tcatccgttc tctgtatcag ccgggcggtg tggcccccga gtctcttgaa tatattgaag    960 

cccatggcac gggcaccaag gtgggggacc cccaggaact gaacggcatt actcggtccc   1020 

tgtgtgcttt ccgccagagc cctttgttaa ttggctccac caaatccaac atgggacacc   1080 

ctgagcctgc ctcggggctt gcagccctga ccaaggtgct gttatcccta gaaaatgggg   1140 

tttgggcccc caacctgcat ttccacaacc ccaaccctga aatcccagca cttcttgatg   1200 

ggcggctgca ggtggtcgat aggcccctgc ctgttcgtgg tggcatcgtg ggcatcaact   1260 

cgtttggctt cggaggtgcc aatgttcacg tcatcctcca gcccaacaca cagcaggccc   1320 

cagcacctgc cccacatgct gccctaccgc atttgctgca tgccagtgga cggaccatgg   1380 

aggcagtgca gggcctgctg gaacagggcc gccagcacag tcaggacttg gcctttgtga   1440 

gcatgctcaa tgacattgca gcaaccccta cagcagccat gcccttcaga ggttacactg   1500 

tgttaggtgt tgagggccat gtccaggaag tgcagcaagt gcctgccagc cagcgcccac   1560 

tctggttcat ctgctcaggg atgggcacac agtggcgtgg aatggggctg agccttatgc   1620 

gcctggacag tttccgtgag tccatcctgc gctctgatga ggctctgaag cccttgggag   1680 

tcaaagtgtc agacctgctg ctgagcactg atgagcacac ctttgatgac atcgtgcatt   1740 

cctttgtgag cctcaccgcc atccagattg ccctcatcga cctgctgacg tctatggggc   1800 

tgaaacctga tggcatcatt gggcactcct tgggagaggt tgcctgtggc tatgcagatg   1860 

gctgtctctc ccagagagag gctgtgcttg cagcctactg gagaggccag tgcattaagg   1920 

atgccaacct tccggctgga tccatggcag ctgttggttt gtcctgggaa gaatgtaaac   1980 

aacgctgccc tcctggtgtg gtgcctgcct gccacaactc tgaggacact gtgaccatct   2040 

ctggacctca ggctgcagtg aatgaatttg tggagcagct aaagcaagag ggcgtgtttg   2100 

ccaaggaggt gcgaacaggt ggcctggcct tccactccta cttcatggaa ggaattgccc   2160 

ccacgctgct gcaggctctc aagaaggtga tccgggagcc acggccacgc tcagcacgct   2220 

ggctcagcac ctctatccct gaggcccagt ggcagagcag cctggcccgc acatcttctg   2280 

ctgagtacaa cgtcaacaac ctggtgagcc ctgtgctctt ccaggaagca ctgtggcacg   2340 

tccccgagca cgccgtggtg ctggagattg caccccatgc actgttgcag gctgtcctga   2400 

agcgaggcgt gaagcctagc tgcaccatca tccccttgat gaagagggac cataaagata   2460 

acttggagtt cttcctcacc aacctcggca aggtgcacct cacaggcatc gacatcaacc   2520 

ctaatgcctt gttcccacct gtggaattcc cggttccccg agggactcct ctcatctccc   2580 

ctcacatcaa gtgggaccac agtcagactt gggatatccc agttgctgaa gacttcccca   2640 

acggttccag ctcctcctca gctacagtct acaacattga cgccagttcc gagtcatctg   2700 

accactacct ggtcgaccac tgcattgacg gccgtgtcct cttccctggc actggctacc   2760 

tgtacctggt gtggaagaca ctggctcgaa gcctgagctt gtccctagaa gagacccctg   2820 

tggtgtttga gaacgtgaca tttcatcagg ccaccatcct gcccaggaca ggaaccgtgc   2880 

ctctggaggt gcggctgcta gaggcctcac atgcatttga ggtgtctgac agtggcaacc   2940 

tgatagtgag cgggaaagtg taccagtggg aagaccctga ctccaagtta ttcgaccacc   3000 

cagaagtccc gatccccgcc gagtccgagt ctgtctcccg cttgacgcag ggagaagtat   3060 

acaaggagct gcggctacgt ggctatgact atggccctca tttccagggc gtctatgagg   3120 

ccaccctcga aggtgagcaa ggcaagctgc tctggaaaga caactgggtg accttcatgg   3180 

acacaatgct gcagatatcc atcctgggct tcagcaagca gagtctgcag ctacccaccc   3240 

gtgtgactgc catctatatt gaccctgcaa cccacctgca gaaggtgtac atgctggagg   3300 

gagacactca agtggctgac gtgaccacga gccgctgtct gggcgtgacc gtctctggtg   3360 

gtgtctacat ttcgagacta cagacaacag caacctcacg gcggcagcag gaacagctgg   3420 

tccccaccct ggagaagttt gtcttcacac cccatgtgga gcctgagtgc ctgtctgaga   3480 

gtgctatcct gcagaaagag ctgcagctgt gcaagggtct ggcaaaggct ctgcagacca   3540 

aggccaccca gcaagggctg aagatgacag tgcctgggct agaggacctt ccccagcatg   3600 

gactgcctcg actcttggct gctgcctgcc agctgcagct caacgggaac ctgcaactgg   3660 

agttaggtga ggtactggct cgagagaggc tcctgctgcc agaagaccct ctgatcagtg   3720 

gcctccttaa ctcccaggcc ctcaaggcct gcatagacac agccctggag aacttgtcta   3780 

ctctcaagat gaaggtggtg gaggtgctgg ctggagaagg ccacttgtat tcccacatct   3840 

cagcactgct caacacccag cctatgctgc aactggagta tacagccacc gaccggcacc   3900 

cccaggccct gaaggatgtt cagaccaagc tgcagcagca tgatgtagca cagggccagt   3960 

gggacccttc tggtcctgct cctaccaacc tgggtgctct tgaccttgtg gtgtgcaact   4020 

gtgcgttagc caccctgggg gatccagccc tggccctgga caacatggta gctgccctca   4080 

aggatggtgg tttcctgcta atgcacacag tgctcaaagg acatgccctt ggggagaccc   4140 

tggcctgcct cccttctgag gtgcagcctg ggcccagctt cttaagccag gaagagtggg   4200 

agagcctgtt ctcaaggaag gcactgcacc tggtgggcct taaaaagtca ttctacggta   4260 

ctgcgctgtt cctgtgccgc cgtctcagcc cacaggacaa gcccatcttc ctgcctgtgg   4320 

aggatactag tttccagtgg gtggactctc tgaagagcat tctggccaca tcctcctccc   4380 

agcctgtgtg gctaacagcc atgaactgcc ccacctcagg tgtggtaggc ttggtgaact   4440 

gtctccgaaa agagccgggt ggacaccgga ttcggtgtat cctgctgtcc aacctcagca   4500 

gcacatctca cgtccccaag ctggaccctg gctcttcaga gctacagaag gtgctagaga   4560 

gtgatctggt gatgaacgtg tacagggacg gtgcctgggg tgccttccgt cacttccagt   4620 

tagagcagga caagcccgag gagcagacag cacatgcctt tgtaaacgtc cttacccgag   4680 

gggaccttgc ctccatccgc tgggtctctt ctcccctgaa acacatgcag ccgccctcga   4740 

gctcaggagc acagctctgc actgtctact atgcctcact gaacttccga gatatcatgc   4800 

tggccacggg caagctgtcc cctgatgcca ttccaggtaa atgggccagc cgggactgca   4860 

tgcttggcat ggagttctca ggccgtgata agtgcggccg gcgtgtgatg gggctggtac   4920 

ccgcagaagg cctggccacc tcagtcctgt tatcacccga cttcctctgg gatgtaccct   4980 

ctagctggac cctggaggag gcggcttctg tgcctgttgt ctacaccacc gcctactact   5040 

ccttagtagt gcgtggtcgt attcagcacg gggaaactgt gctcattcac tcgggctccg   5100 

gtggtgtggg ccaagcggcc atttccattg cccttagcct gggctgccga gtcttcacca   5160 

ctgtgggctc cgctgagaag cgagcttacc tccaggccag attccctcag ctggatgaca   5220 

ccagctttgc taactctcga gacacatcgt ttgagcagca tgtgttactg cacacaggtg   5280 

gcaaaggggt ggacctggtc ctcaactccc tggcagaaga gaagctgcag gccagtgtgc   5340 

ggtgcttggc tcagcatggc cgcttcctag agatcggcaa atttgatctt tctaacaacc   5400 

accctctggg catggccatc ttcttgaaga acgtcacttt ccatgggatc ctgctggatg   5460 

cactttttga gggggccaac gacagctggc gggaggtggc agagctgctg aaggccggca   5520 

tccgtgatgg ggttgtgaag cctctcaagt gtacagtgtt tcccaaggcc caggtggagg   5580 

acgccttccg atacatggct caaggaaaac atattggcaa agtccttgtc caggtacggg   5640 

aggaggagcc cgaggctatg ctgccagggg ctcagcccac cctgatttcc gccatctcca   5700 

agaccttctg cccagagcat aagagttaca tcatcactgg tggcctaggt ggctttggcc   5760 

tggaactggc ccggtggctt gtgcttcgtg gggcccaaag gcttgtacta acttcccgat   5820 

ctggaatccg cacaggctac caagccaagc acgttcggga gtggaggcgc cagggcatcc   5880 

atgtgctagt gtcgacaagc aatgtcagtt cactggaggg ggcccgtgct ctcatcgctg   5940 

aagccacaaa gcttgggccc gttggaggtg tcttcaacct ggccatggtt ttaagggatg   6000 

ccatgctgga gaaccagact ccagaactct tccaggatgt caacaagccc aagtacaatg   6060 

gcaccctgaa ccttgacagg gcgacccggg aagcctgtcc tgagctggac tactttgtgg   6120 

ccttctcctc tgtaagctgc gggcgtggta atgctggcca atccaactat ggcttcgcca   6180 

actctaccat ggagcgtatt tgcgaacagc gccggcacga tggcctccca ggtcttgccg   6240 

tgcaatgggg tgccattggt gacgtgggca ttatcttgga agcgatgggt accaatgaca   6300 

cagtcgttgg cggcacactg ccacagcgca tctcctcctg catggaggtg ctggacctct   6360 

tcctgaatca gccccacgca gtcctgagca gttttgtgct ggctgagaag aaagctgtgg   6420 

cccatggtga tggtgaagcc cagagggatc tggtgaaagc agtggcacac atcctaggca   6480 

tccgcgacct cgcagggatt aacctggaca gctcgctggc agacctcggc ctggactcgc   6540 

tcatgggtgt ggaagtgcgc cagatcctgg aacgtgaaca tgatctggtg ctacccattc   6600 

gtgaagtacg gcaactcaca ctgcggaagc ttcaggaaat gtcctccaag gctggctcag   6660 

acactgagtt ggcagccccc aagtccaaga atgatacatc cctgaagcag gcccagctga   6720 

atctgagtat cctgctggtg aaccctgagg gccctacctt aacacgactc aactcagtgc   6780 

agagctctga gcggcctctg ttcctggtgc accccattga aggttccatc actgtgttcc   6840 

acagcctggc tgccaagctc agtgtgccca cctacggtct gcagtgcacc caagcggccc   6900 

ccctggacag cattccaaac ctggctgcct actacattga ttgcatcaag caggtgcagc   6960 

ctgaggggcc ctaccgagtg gctgggtatt cttttggagc ttgtgtagcc ttcgagatgt   7020 

gctcccagct gcaggcccag cagggcccag cccccgccca caacaacctc ttcttgtttg   7080 

atggctcaca cacctacgta ttggcgtaca cccagagcta ccgggcaaag ctgaccccag   7140 

gctgtgaggc tgaggctgaa gctgaagcca tatgcttctt cattaagcag tttgttgatg   7200 

cagagcatag caaggtgcta gaggccctgc taccactgaa gagcctggag gaccgggttg   7260 

ctgctgctgt ggacctcatc actagaagcc accagagcct ggaccgccgt gacctgagct   7320 

ttgctgccgt gtccttctac tacaagcttc gagccgccga ccagtataaa cccaaggcca   7380 

agtaccacgg caatgtgatc ctgctgcggg ccaagacagg tggcacctac ggcgaggact   7440 

tgggtgccga ttacaacctg tcccaggtgt gtgatgggaa ggtgtctgtg cacatcattg   7500 

agggtgacca ccgtacgctg ctggagggca ggggcctgga gtctatcatc aacatcatcc   7560 

acagctccct ggctgagcct cgagtgagtg tacgggaggg ctagacctgc ctaccatgaa   7620 

gccacgaccc acaccggcca ccagagatgc tccgatcccc accacaccct gagtgcaggg   7680 

actggggagg gtcctgctgg tgggaccccc tcaccccagt ggcccagcac caccccctcc   7740 

cctggtggct gctacaaaca ggaccatcac atgtgtccca gccacttagt ggggttccca   7800 

gagccactga cttggaggca ccctggtctg tgaagagtca gtggaggcca gcaagagcca   7860 

aactgagcct tttctgccaa gtgacatttg tcacactggt tgtttctcca ttaaattctc   7920 

atatttattg cattgctggg aaagaccgcc caccccaggg ttaactcatt ccagaacccc   7980 

taaagtggga aaagccatgt ggggaaggct gctggctgga gccccttttt gtcttagccc   8040 

tgtacccgct cactgcaggg cagggtatgg agagggctgg ttcgcgggga acgaggaccc   8100 

cagcagacac tgtagcccat ggcccttggt ccccagcact cccggctgca cccatgatgc   8160 

agggcctacc agactctgcg gaccgcaccg ggcactcact gtatttgttt tccaagattc   8220 

aaattgctgc ttgggttttg aatttactgc agctgtcagt gtaaagaaac atgtctgaac   8280 

tgtgtccttt ttacaccaac ctggtaaaaa tgctcttgat gctgtcccgt tgccacaatt   8340 

aaactgcacg tgagctctgg cttccgttca gtctctttcc agtcccagac ctgagtcccc   8400 

agagcctcca cagctcttac agtgagaatc aaattggccc actccttgga aggcgtggca   8460 

ttctgtcaga gtaaaaggaa agtagagtgt gctgattcac gttcagcgtg tggggctggc   8520 

tagagacctt ggcactgtag tgaacagaat gtgtccacct ttaagtcacc ctgaaggcat   8580 

caccatagct acagcctcac ccaggggtag agaatagtac tgtctacttg ttgactacct   8640 

ggcagttggt gccagcccct atagaggaaa acagcagtgt gtggccactg tgagaagcat   8700 

atccctggaa acaggtgacc agagcagagg gctaacgcct acctgagtca cacaaaactg   8760 

accaggcttg agtgtccaga agagtctatc agaaggccac agcattcagt cctatccaca   8820 

gagagcagca gactaagttg tctccttgcc agcttagaaa actgcagtgc tggggtacag   8880 

gtagggtgtt caggaggtcc gggccccagt gattagtcta agactgaagc atctggttgg   8940 

ctgtggtccc acctagaaaa ttcttaaagc tcttgtcatg tacttcctgg gaaggaccta   9000 

ccctgtctca ataatgtctc tagctcgttg gagtctactg actcaaacat ttataaagtg   9060 

tcctagaaag gcctgactcc cctacaaggc tgtgtgatcc ttcaaactca catatgtgag   9120 

ccaataaaac cttgagactc tag                                           9143 

 
           
             22  
             373  
             PRT  
             Rattus norvegicus  
             
               Glutamine synthetase  
             
           
            22 

Met Ala Thr Ser Ala Ser Ser His Leu Asn Lys Gly Ile Lys Gln Met 
1               5                   10                  15 
Tyr Met Asn Leu Pro Gln Gly Glu Lys Ile Gln Leu Met Tyr Ile Trp 
            20                  25                  30 
Val Asp Gly Thr Gly Glu Gly Leu Arg Cys Lys Thr Arg Thr Leu Asp 
        35                  40                  45 
Cys Asp Pro Lys Cys Val Glu Glu Leu Pro Glu Trp Asn Phe Asp Gly 
    50                  55                  60 
Ser Ser Thr Phe Gln Ser Glu Gly Ser Asn Ser Asp Met Tyr Leu His 
65                  70                  75                  80 
Pro Val Ala Met Phe Arg Asp Pro Phe Arg Arg Asp Pro Asn Lys Leu 
                85                  90                  95 
Val Phe Cys Glu Val Phe Lys Tyr Asn Arg Lys Pro Ala Glu Thr Asn 
            100                 105                 110 
Leu Arg His Ser Cys Lys Arg Ile Met Asp Met Val Ser Ser Gln Arg 
        115                 120                 125 
Pro Trp Phe Gly Met Glu Gln Glu Tyr Thr Leu Met Gly Thr Asp Gly 
    130                 135                 140 
His Pro Phe Gly Trp Pro Ser Asn Gly Phe Pro Gly Pro Gln Gly Pro 
145                 150                 155                 160 
Tyr Tyr Cys Gly Val Gly Ala Asp Lys Ala Tyr Gly Arg Asp Ile Val 
                165                 170                 175 
Glu Ala His Tyr Arg Ala Cys Leu Tyr Ala Gly Ile Lys Ile Thr Gly 
            180                 185                 190 
Thr Asn Ala Glu Val Met Pro Ala Gln Trp Glu Phe Gln Ile Gly Pro 
        195                 200                 205 
Cys Glu Gly Ile Arg Met Gly Asp His Leu Trp Val Ala Arg Phe Ile 
    210                 215                 220 
Leu His Arg Val Cys Glu Asp Phe Gly Val Ile Ala Thr Phe Asp Pro 
225                 230                 235                 240 
Lys Pro Ile Pro Gly Asn Trp Asn Gly Ala Gly Cys His Thr Asn Phe 
                245                 250                 255 
Ser Thr Lys Ala Met Arg Glu Glu Asn Gly Leu Arg Cys Ile Glu Glu 
            260                 265                 270 
Ala Ile Asp Lys Leu Ser Lys Arg His Gln Tyr His Ile Arg Ala Tyr 
        275                 280                 285 
Asp Pro Lys Gly Gly Leu Asp Asn Ala Arg Arg Leu Thr Gly Phe His 
    290                 295                 300 
Glu Thr Ser Asn Ile Asn Asp Phe Ser Ala Gly Val Ala Asn Arg Ser 
305                 310                 315                 320 
Ala Ser Ile Arg Ile Pro Arg Ile Val Gly Gln Glu Lys Lys Gly Tyr 
                325                 330                 335 
Phe Glu Asp Arg Arg Pro Ser Ala Asn Cys Asp Pro Tyr Ala Val Thr 
            340                 345                 350 
Glu Ala Ile Val Arg Thr Cys Leu Leu Asn Glu Thr Gly Asp Glu Pro 
        355                 360                 365 
Phe Gln Tyr Lys Asn 
    370 

 
           
             23  
             2793  
             DNA  
             Rattus norvegicus  
             
               Glutamine synthetase  
             
           
            23 

acagccgaga atgggagtag ggcggagtgt ttgagcagca cacccatttc ctctccgctc     60 

ttcgtctcgt tctcgtggcc tgtccaccca tccatcatct gccggccacc gctctgaaca    120 

ccttccacca tggccacctc agcaagttcc cacttgaaca aaggcatcaa gcagatgtac    180 

atgaacctgc cccagggcga gaagatccaa ctcatgtata tctgggttga tggtaccggg    240 

gaagggctac gctgcaagac ccgtactctg gactgtgacc ccaagtgtgt agaagagtta    300 

cccgagtgga actttgatgg ttctagtacg tttcagtctg aaggctccaa cagcgacatg    360 

tacctccatc ctgtggccat gtttcgagac cccttccgca gagaccccaa caagctggtg    420 

ttctgcgaag tattcaagta taaccggaag cccgcagaga ccaacctgag gcacagctgt    480 

aagcgtataa tggacatggt gagcagccag cgcccctggt ttggaatgga acaggagtat    540 

actctcatgg gaacagacgg ccaccctttc ggctggcctt ctaatggctt ccctggaccc    600 

caaggaccct attactgcgg tgtgggagct gacaaggctt atggccgaga tatcgtggag    660 

gctcactacc gggcctgctt gtatgctgga atcaagatca cagggacaaa tgccgaggtt    720 

atgcctgccc agtgggaatt ccagatagga ccctgcgaag ggatccgcat gggagatcat    780 

ctctgggtag cccgttttat cttgcatcgg gtatgcgaag actttggggt gatagcaacc    840 

tttgacccca agcccattcc agggaactgg aatggggcag gctgccacac caactttagc    900 

accaaggcca tgcgggagga gaatggtctg aggtgcattg aggaggccat tgataaactg    960 

agcaagaggc accagtacca catccgtgcc tacgacccca aggggggcct ggacaacgcc   1020 

cgccgtctga ctggattcca cgaaacctcc aacatcaacg acttttccgc tggcgttgcc   1080 

aaccgcagcg ccagtatccg cattccccgg attgtcggcc aggagaagaa gggttacttt   1140 

gaagaccgtc ggccttctgc caattgcgac ccctatgcgg tgacggaagc catcgtccgc   1200 

acgtgtctcc tcaacgaaac tggcgacgag cccttccaat acaagaacta agcggactcg   1260 

acttccagtg atcttgagcc cttcctagtt caccccactc ccaactgttc cctctcccac   1320 

tggtccccac tgtaactcaa aaggatggaa taccaaggtc tttttattcc ttgcgcccag   1380 

ttaatttttg cctttattgg tcagaataga ggggtcaggt tcttaatctc tacacaccca   1440 

accccttctt tcctagctag ctttccagtg ggggaacggg agggggtggg gaagggtaac   1500 

ccaccgcttc atctcagcgg gaatgcatgt cctgtaggca tagctgtcac aaatcgggtg   1560 

tacttgtggt gagggaggac tggttttttt tttccttcag gataattgaa agggcaggcc   1620 

caacagctta gattaacatt ttctctgtca gtagagagct gttatttctt ccggtgaaac   1680 

cagctttcta ttgaagtctg gtgaggagtt ggaggttggt ctcttggctt ccttagctta   1740 

gggaagggga gttcaccctc ccttcatgaa acacagttca cctgacaaat ggccctactg   1800 

taaaggaaga aaaaagtttc ttggtcctcc atttataact caaagcagag tagtattttt   1860 

atatttaaat gttaaaaaca aaaaagttat atatatgggt gtgtggatat atatgtcttt   1920 

tctaattgag aaaaccatcc tattccctgg gtgccaagtt tgagtgagga gctcggtgta   1980 

gaagtgaggc actcttgagg taggggtggg gatgcagtac tgggaaagtt ggttatcttg   2040 

ggggttcagc ttcattacta cttagggttt ccctgcccac tctgcaggag cagatgttgg   2100 

acaggtagcc agtgggatgc cactgcttgc cgccactgtc cctgggctta gtttaagggg   2160 

acgtgtatac ctaatccaca cacgagttag aagtatgagt tggctggtca acttgaacat   2220 

tgttacaggc gggtgggtgt tagtgggggg ttattttttg gtgggactag catgtcacta   2280 

aagcgggcct tttgatatat taaatttttt aaagcaaaac aagtttagat tttaatcaag   2340 

ttcgtagggt ttctaacttt acagaattgc ctgtttgttt caatgactcc ttccacttgg   2400 

ctcttagggg aactgaggac aggcctggag ttaatacact tgtcattctg tgtcctagtg   2460 

tcctcttcct ccggcagact gtccccttcc ttctgaaaaa gccgatagag tcttgtttta   2520 

tttttctttt ataataaaca caccccacct ccatcccagc ttgttgcctt gcagttttct   2580 

ggatgtttgt gtcggcagca ggcagctgtg gtttttttct cttgccacga tgactctaat   2640 

taccatgtat agtatgttca gttagataac tcactgtaaa cagactgtaa ctgagagcag   2700 

agcttgtaaa tcaacctaac gtttataaga tttcctctga cttgtttctt tgtggttcca   2760 

aaaaaaaaaa aaaaaaaaaa aacctcaaaa act                                2793 

 
           
             24  
             514  
             PRT  
             Homo sapiens  
             
               Seryl t-RNA synthetase  
             
           
            24 

Met Val Leu Asp Leu Asp Leu Phe Arg Val Asp Lys Gly Gly Asp Pro 
1               5                   10                  15 

Ala Leu Ile Arg Glu Thr Gln Glu Lys Arg Phe Lys Asp Pro Gly Leu 
            20                  25                  30 

Val Asp Gln Leu Val Lys Ala Asp Ser Glu Trp Arg Arg Cys Arg Phe 
        35                  40                  45 

Arg Ala Asp Asn Leu Ser Lys Leu Lys Asn Leu Cys Ser Lys Thr Ile 
    50                  55                  60 

Gly Glu Lys Met Lys Lys Lys Glu Pro Val Gly Asp Asp Glu Ser Val 
65                  70                  75                  80 

Pro Glu Asn Val Leu Ser Phe Asp Asp Leu Thr Ala Asp Ala Leu Ala 
                85                  90                  95 

Asn Leu Lys Val Ser Gln Ile Lys Lys Val Arg Leu Leu Ile Asp Glu 
            100                 105                 110 

Ala Ile Leu Lys Cys Asp Ala Glu Arg Ile Lys Leu Glu Ala Glu Arg 
        115                 120                 125 

Phe Glu Asn Leu Arg Glu Ile Gly Asn Leu Leu His Pro Ser Val Pro 
    130                 135                 140 

Ile Ser Asn Asp Glu Asp Val Asp Asn Lys Val Glu Arg Ile Trp Gly 
145                 150                 155                 160 

Asp Cys Thr Val Arg Lys Lys Tyr Ser His Val Asp Leu Val Val Met 
                165                 170                 175 

Val Asp Gly Phe Glu Gly Glu Lys Gly Ala Val Val Ala Gly Ser Arg 
            180                 185                 190 

Gly Tyr Phe Leu Lys Gly Val Leu Val Phe Leu Glu Gln Ala Leu Ile 
        195                 200                 205 

Gln Tyr Ala Leu Arg Thr Leu Gly Ser Arg Gly Tyr Ile Pro Ile Tyr 
    210                 215                 220 

Thr Pro Phe Phe Met Arg Lys Glu Val Met Gln Glu Val Ala Gln Leu 
225                 230                 235                 240 

Ser Gln Phe Asp Glu Glu Leu Tyr Lys Val Ile Gly Lys Gly Ser Glu 
                245                 250                 255 

Lys Ser Asp Asp Asn Ser Tyr Asp Glu Lys Tyr Leu Ile Ala Thr Ser 
            260                 265                 270 

Glu Gln Pro Ile Ala Ala Leu His Arg Asp Glu Trp Leu Arg Pro Glu 
        275                 280                 285 

Asp Leu Pro Ile Lys Tyr Ala Gly Leu Ser Thr Cys Phe Arg Gln Glu 
    290                 295                 300 

Val Gly Ser His Gly Arg Asp Thr Arg Gly Ile Phe Arg Val His Gln 
305                 310                 315                 320 

Phe Glu Lys Ile Glu Gln Phe Val Tyr Ser Ser Pro His Asp Asn Lys 
                325                 330                 335 

Ser Trp Glu Met Phe Glu Glu Met Ile Thr Thr Ala Glu Glu Phe Tyr 
            340                 345                 350 

Gln Ser Leu Gly Ile Pro Tyr His Ile Val Asn Ile Val Ser Gly Ser 
        355                 360                 365 

Leu Asn His Ala Ala Ser Lys Lys Leu Asp Leu Glu Ala Trp Phe Pro 
    370                 375                 380 

Gly Ser Gly Ala Phe Arg Glu Leu Val Ser Cys Ser Asn Cys Thr Asp 
385                 390                 395                 400 

Tyr Gln Ala Arg Arg Leu Arg Ile Arg Tyr Gly Gln Thr Lys Lys Met 
                405                 410                 415 

Met Asp Lys Val Glu Phe Val His Met Leu Asn Ala Thr Met Cys Ala 
            420                 425                 430 

Thr Thr Arg Thr Ile Cys Ala Ile Leu Glu Asn Tyr Gln Thr Glu Lys 
        435                 440                 445 

Gly Ile Thr Val Pro Glu Lys Leu Lys Glu Phe Met Pro Pro Gly Leu 
    450                 455                 460 

Gln Glu Leu Ile Pro Phe Val Lys Pro Ala Pro Ile Glu Gln Glu Pro 
465                 470                 475                 480 

Ser Lys Lys Gln Lys Lys Gln His Glu Gly Ser Lys Lys Lys Ala Ala 
                485                 490                 495 

Ala Arg Asp Val Thr Leu Glu Asn Arg Leu Gln Asn Met Glu Val Thr 
            500                 505                 510 

Asp Ala 

 
           
             25  
             1846  
             DNA  
             Homo sapiens  
             
               Seryl t-RNA synthetase  
             
           
            25 

gcagtgcggc ggtcacaggc tgagtgctgc ggcgcgatcc ttgcttccct gagcgttggc     60 

ccgggaggaa agaagatggt gctggatctg gatttgtttc gggtggataa aggaggggac    120 

ccagccctca tccgagagac gcaggagaag cgcttcaagg acccgggact agtggaccag    180 

ctggtgaagg cagacagcga gtggcgacga tgtagatttc gggcagacaa cttgagcaag    240 

ctgaagaacc tatgcagcaa gacaatcgga gagaaaatga agaaaaaaga gccagtggga    300 

gatgatgagt ctgtcccaga gaatgtgctg agtttcgatg accttactgc agacgcttta    360 

gctaacctga aagtctcaca aatcaaaaaa gtccgactcc tcattgatga agccatcctg    420 

aagtgtgacg cggagcggat aaagttggaa gcagagcggt ttgagaacct ccgagagatt    480 

gggaaccttc tgcacccttc tgtacccatc agtaacgatg aggatgtgga caacaaagta    540 

gagaggattt ggggcgattg tacagtcagg aagaagtact ctcatgtgga cctggtggtg    600 

atggtagatg gctttgaagg cgaaaagggg gccgtggtgg ctgggagtcg agggtacttc    660 

ttgaaggggg tcctggtgtt cctggaacag gctctcatcc agtatgccct tcgcaccttg    720 

ggaagtcggg gctacattcc catttatacc ccctttttca tgaggaagga ggtcatgcag    780 

gaggtggcac agctcagcca gtttgatgaa gaactttata aggtgattgg caaaggcagt    840 

gaaaagtctg atgacaactc ctatgatgag aagtacctga ttgccacctc agagcagccc    900 

attgctgccc tgcaccggga tgagtggctc cggccggagg acctgcccat caagtatgct    960 

ggcctgtcta cctgcttccg tcaggaggtg ggctcccatg gccgtgacac ccgtggcatc   1020 

ttccgagtcc atcagtttga gaagattgaa cagtttgtgt actcatcacc ccatgacaac   1080 

aagtcatggg agatgtttga agagatgatt accaccgcag aggagttcta ccagtccctg   1140 

gggattcctt accacattgt gaatattgtc tcaggttctt tgaatcatgc tgccagtaag   1200 

aagcttgacc tggaggcctg gtttccgggc tcaggagcct tccgtgagtt ggtctcctgt   1260 

tctaattgca cggattacca ggctcgccgg cttcgaatcc gatatgggca aaccaagaag   1320 

atgatggaca aggtggagtt tgtccatatg ctcaatgcta ccatgtgcgc cactacccgt   1380 

accatctgcg ccatcctgga gaactaccag acagagaagg gcatcactgt gcctgagaaa   1440 

ttgaaggagt tcatgccgcc aggactgcaa gaactgatcc cctttgtgaa gcctgcgccc   1500 

attgagcagg agccatcaaa gaagcagaag aagcaacatg agggcagcaa aaagaaagca   1560 

gcagcaagag acgtcaccct agaaaacagg ctgcagaaca tggaggtcac cgatgcttga   1620 

acattcctgc ctccctattt gccaggcttt catttctgtc tgctgagatc tcagagcctg   1680 

cccaacagca gggaagccaa gcacccattc atccccctgc ccccatctga ctgcgtagct   1740 

gagaggggaa cagtgccatg taccacacag atgttcctgt ctcctcgcat gggcataggg   1800 

acccatcatt gatgactgat gaaaccatgt aataaagcat ctctgg                  1846 

 
           
             26  
             434  
             PRT  
             Homo sapiens  
             
               Alpha-enolase  
             
           
            26 

Met Ser Ile Leu Lys Ile His Ala Arg Glu Ile Phe Asp Ser Arg Gly 
1               5                   10                  15 

Asn Pro Thr Val Glu Val Asp Leu Phe Thr Ser Lys Gly Leu Phe Arg 
            20                  25                  30 

Ala Ala Val Pro Ser Gly Ala Ser Thr Gly Ile Tyr Glu Ala Leu Glu 
        35                  40                  45 

Leu Arg Asp Asn Asp Lys Thr Arg Tyr Met Gly Lys Gly Val Ser Lys 
    50                  55                  60 

Ala Val Glu His Ile Asn Lys Thr Ile Ala Pro Ala Leu Val Ser Lys 
65                  70                  75                  80 

Lys Leu Asn Val Thr Glu Gln Glu Lys Ile Asp Lys Leu Met Ile Glu 
                85                  90                  95 

Met Asp Gly Thr Glu Asn Lys Ser Lys Phe Gly Ala Asn Ala Ile Leu 
            100                 105                 110 

Gly Val Ser Leu Ala Val Cys Lys Ala Gly Ala Val Glu Lys Gly Val 
        115                 120                 125 

Pro Leu Tyr Arg His Ile Ala Asp Leu Ala Gly Asn Ser Glu Val Ile 
    130                 135                 140 

Leu Pro Val Pro Ala Phe Asn Val Ile Asn Gly Gly Ser His Ala Gly 
145                 150                 155                 160 

Asn Lys Leu Ala Met Gln Glu Phe Met Ile Leu Pro Val Gly Ala Ala 
                165                 170                 175 

Asn Phe Arg Glu Ala Met Arg Ile Gly Ala Glu Val Tyr His Asn Leu 
            180                 185                 190 

Lys Asn Val Ile Lys Glu Lys Tyr Gly Lys Asp Ala Thr Asn Val Gly 
        195                 200                 205 

Asp Glu Gly Gly Phe Ala Pro Asn Ile Leu Glu Asn Lys Glu Gly Leu 
    210                 215                 220 

Glu Leu Leu Lys Thr Ala Ile Gly Lys Ala Gly Tyr Thr Asp Lys Val 
225                 230                 235                 240 

Val Ile Gly Met Asp Val Ala Ala Ser Glu Phe Phe Arg Ser Gly Lys 
                245                 250                 255 

Tyr Asp Leu Asp Phe Lys Ser Pro Asp Asp Pro Ser Arg Tyr Ile Ser 
            260                 265                 270 

Pro Asp Gln Leu Ala Asp Leu Tyr Lys Ser Phe Ile Lys Asp Tyr Pro 
        275                 280                 285 

Val Val Ser Ile Glu Asp Pro Phe Asp Gln Asp Asp Trp Gly Ala Trp 
    290                 295                 300 

Gln Lys Phe Thr Ala Ser Ala Gly Ile Gln Val Val Gly Asp Asp Leu 
305                 310                 315                 320 

Thr Val Thr Asn Pro Lys Arg Ile Ala Lys Ala Val Asn Glu Lys Ser 
                325                 330                 335 

Cys Asn Cys Leu Leu Leu Lys Val Asn Gln Ile Gly Ser Val Thr Glu 
            340                 345                 350 

Ser Leu Gln Ala Cys Lys Leu Ala Gln Ala Asn Gly Trp Gly Val Met 
        355                 360                 365 

Val Ser His Arg Ser Gly Glu Thr Glu Asp Thr Phe Ile Ala Asp Leu 
    370                 375                 380 

Val Val Gly Leu Cys Thr Gly Gln Ile Lys Thr Gly Ala Pro Cys Arg 
385                 390                 395                 400 

Ser Glu Arg Leu Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu Glu Glu 
                405                 410                 415 

Leu Gly Ser Lys Ala Lys Phe Ala Gly Arg Asn Phe Arg Asn Pro Leu 
            420                 425                 430 

Ala Lys 

 
           
             27  
             1755  
             DNA  
             Homo sapiens  
             
               Alpha-enolase  
             
           
            27 

acggagatct cgccggcttt acgttcacct cggtgtctgc agcaccctcc gcttcctctc     60 

ctaggcgacg agacccagtg gctagaagtt caccatgtct attctcaaga tccatgccag    120 

ggagatcttt gactctcgcg ggaatcccac tgttgaggtt gatctcttca cctcaaaagg    180 

tctcttcaga gctgctgtgc ccagtggtgc ttcaactggt atctatgagg ccctagagct    240 

ccgggacaat gataagactc gctatatggg gaagggtgtc tcaaaggctg ttgagcacat    300 

caataaaact attgcgcctg ccctggttag caagaaactg aacgtcacag aacaagagaa    360 

gattgacaaa ctgatgatcg agatggatgg aacagaaaat aaatctaagt ttggtgcgaa    420 

cgccattctg ggggtgtccc ttgccgtctg caaagctggt gccgttgaga agggggtccc    480 

cctgtaccgc cacatcgctg acttggctgg caactctgaa gtcatcctgc cagtcccggc    540 

gttcaatgtc atcaatggcg gttctcatgc tggcaacaag ctggccatgc aggagttcat    600 

gatcctccca gtcggtgcag caaacttcag ggaagccatg cgcattggag cagaggttta    660 

ccacaacctg aagaatgtca tcaaggagaa atatgggaaa gatgccacca atgtggggga    720 

tgaaggcggg tttgctccca acatcctgga gaataaagaa ggcctggagc tgctgaagac    780 

tgctattggg aaagctggct acactgataa ggtggtcatc ggcatggacg tagcggcctc    840 

cgagttcttc aggtctggga agtatgacct ggacttcaag tctcccgatg accccagcag    900 

gtacatctcg cctgaccagc tggctgacct gtacaagtcc ttcatcaagg actacccagt    960 

ggtgtctatc gaagatccct ttgaccagga tgactgggga gcttggcaga agttcacagc   1020 

cagtgcagga atccaggtag tgggggatga tctcacagtg accaacccaa agaggatcgc   1080 

caaggccgtg aacgagaagt cctgcaactg cctcctgctc aaagtcaacc agattggctc   1140 

cgtgaccgag tctcttcagg cgtgcaagct ggcccaggcc aatggttggg gcgtcatggt   1200 

gtctcatcgt tcgggggaga ctgaagatac cttcatcgct gacctggttg tggggctgtg   1260 

cactgggcag atcaagactg gtgccccttg ccgatctgag cgcttggcca agtacaacca   1320 

gctcctcaga attgaagagg agctgggcag caaggctaag tttgccggca ggaacttcag   1380 

aaaccccttg gccaagtaag ctgtgggcag gcaagccttc ggtcacctgt tggctacaca   1440 

gacccctccc ctcgtgtcag ctcaggcagc tcgaggcccc cgaccaacac ttgcaggggt   1500 

ccctgctagt tagcgcccca ccgccgtgga gttcgtaccg cttccttaga acttctacag   1560 

aagccaagct ccctggagcc ctgttggcag ctctagcttt tgcagtcgtg taatgggccc   1620 

aagtcattgt ttttctcgcc tcactttcca ccaagtgtct agagtcatgt gagcctcgtg   1680 

tcatctccgg ggtggccaca ggctagatcc ccggtggttt tgtgctcaaa ataaaaagcc   1740 

tcagtgaccc atgag                                                    1755 

 
           
             28  
             316  
             PRT  
             Rattus norvegicus  
             
               Aldose reductase  
             
           
            28 

Met Ala Ser His Leu Glu Leu Asn Asn Gly Thr Lys Met Pro Thr Leu 
1               5                   10                  15 

Gly Leu Gly Thr Trp Lys Ser Pro Pro Gly Gln Val Thr Glu Ala Val 
            20                  25                  30 

Lys Val Ala Ile Asp Met Gly Tyr Arg His Ile Asp Cys Ala Gln Val 
        35                  40                  45 

Tyr Gln Asn Glu Lys Glu Val Gly Val Ala Leu Gln Glu Lys Leu Lys 
    50                  55                  60 

Glu Gln Val Val Lys Arg Gln Asp Leu Phe Ile Val Ser Lys Leu Trp 
65                  70                  75                  80 

Cys Thr Phe His Asp Gln Ser Met Val Lys Gly Ala Cys Gln Lys Thr 
                85                  90                  95 

Leu Ser Asp Leu Gln Leu Asp Tyr Leu Asp Leu Tyr Leu Ile His Trp 
            100                 105                 110 

Pro Thr Gly Phe Lys Pro Gly Pro Asp Tyr Phe Pro Leu Asp Ala Ser 
        115                 120                 125 

Gly Asn Val Ile Pro Ser Asp Thr Asp Phe Val Asp Thr Trp Thr Ala 
    130                 135                 140 

Met Glu Gln Leu Val Asp Glu Gly Leu Val Lys Ala Ile Gly Val Ser 
145                 150                 155                 160 

Asn Phe Asn Pro Leu Gln Ile Glu Arg Ile Leu Asn Lys Pro Gly Leu 
                165                 170                 175 

Lys Tyr Lys Pro Ala Val Asn Gln Ile Glu Cys His Pro Tyr Leu Thr 
            180                 185                 190 

Gln Glu Lys Leu Ile Glu Tyr Cys His Cys Lys Gly Ile Val Val Thr 
        195                 200                 205 

Ala Tyr Ser Pro Leu Gly Ser Pro Asp Arg Pro Trp Ala Lys Pro Glu 
    210                 215                 220 

Asp Pro Ser Leu Leu Glu Asp Pro Arg Ile Lys Glu Ile Ala Ala Lys 
225                 230                 235                 240 

Tyr Asn Lys Thr Thr Ala Gln Val Leu Ile Arg Phe Pro Ile Gln Arg 
                245                 250                 255 

Asn Leu Val Val Ile Pro Lys Ser Val Thr Pro Ala Arg Ile Ala Glu 
            260                 265                 270 

Asn Phe Lys Val Phe Asp Phe Glu Leu Ser Asn Glu Asp Met Ala Thr 
        275                 280                 285 

Leu Leu Ser Tyr Asn Arg Asn Trp Arg Val Cys Ala Leu Met Ser Cys 
    290                 295                 300 

Ala Lys His Lys Asp Tyr Pro Phe His Ala Glu Val 
305                 310                 315 

 
           
             29  
             1337  
             DNA  
             Rattus norvegicus  
             
               Aldose reductase  
             
           
            29 

ctcttgcggg tcgttgtgcg taacttgcag caatcatggc tagccatctg gaactcaaca     60 

acggcaccaa gatgcccacc ctgggtctgg gcacctggaa gtctcctcct ggccaggtga    120 

ccgaggctgt gaaggttgct atcgacatgg ggtatcgcca cattgactgc gcccaggtgt    180 

accagaatga gaaggaggtg ggggtggccc tccaggagaa gctcaaggag caggtggtga    240 

agcgccagga tctcttcatt gtcagcaagc tgtggtgcac gttccacgac cagagcatgg    300 

tgaaaggggc ctgccagaag acgctgagcg acctgcagct ggactacctg gacctctacc    360 

ttattcactg gccaactggc ttcaagcctg ggcctgacta tttccccctg gatgcatcgg    420 

gaaacgtgat tcctagtgac accgattttg tggacacttg gacggctatg gagcaactag    480 

tggatgaagg tttggtaaaa gcaatcggag tctccaactt caaccctctt cagattgaga    540 

ggatcttgaa caaacctggc ttaaagtata agcctgctgt taaccagatc gagtgccacc    600 

catacctaac tcaggagaag ctgattgagt actgccattg caaaggcatc gtggtgactg    660 

catacagtcc ccttggttct cctgacaggc cctgggccaa gcctgaggac ccctctctcc    720 

tggaggatcc caggatcaag gaaattgcag ccaagtacaa taaaactaca gcccaggtgc    780 

tgatccggtt ccccatccaa aggaacctgg tcgtgatccc caagtctgtg acaccagcac    840 

gtattgctga gaactttaag gtctttgact ttgagctgag caatgaggac atggccactc    900 

tactcagcta caacaggaac tggagggtgt gcgccttgat gagctgtgcc aaacacaagg    960 

attacccctt ccacgcagaa gtctgaagct gtggtggacg aatcctgctc ctccccaagc   1020 

gacttaacac atgttctttc tgcctcatct gcccttgcaa gtgtccctct gcactgggtg   1080 

gcaccttgca gaccagatgg tgagagtttg ttagtttgac gtagaatgtg gagggcagta   1140 

ccagtagctg aggagtttct tcggcctttc ttggtcttct tcccacctgg aggactttaa   1200 

cacgagtacc ttttccaacc aaagagaaag caagatttat agcccaagtc atgccactaa   1260 

cacttaaatt tgagtgctta gaactccagt cctatggggg tcagactttt tgcctcaaat   1320 

aaaaactgct tttgtcg                                                  1337 

 
           
             30  
             299  
             PRT  
             Rattus norvegicus  
             
               Cytochrome C oxidase, subunit 1  
             
           
            30 

Glu Phe Pro Phe Phe Asp Pro Ala Gly Gly Gly Asp Pro Ile Leu Tyr 
1               5                   10                  15 

Gln His Leu Phe Trp Phe Phe Gly His Pro Glu Val Tyr Ile Leu Ile 
            20                  25                  30 

Leu Pro Gly Phe Gly Ile Ile Ser His Val Val Thr Tyr Tyr Ser Gly 
        35                  40                  45 

Lys Lys Glu Pro Phe Gly Tyr Met Gly Met Val Trp Ala Met Met Ser 
    50                  55                  60 

Ile Gly Phe Leu Gly Phe Ile Val Trp Ala His His Met Phe Thr Val 
65                  70                  75                  80 

Gly Leu Asp Val Asp Thr Arg Ala Tyr Phe Thr Ser Ala Thr Met Ile 
                85                  90                  95 

Ile Ala Ile Pro Thr Gly Val Lys Val Phe Ser Trp Leu Ala Thr Leu 
            100                 105                 110 

His Gly Gly Asn Ile Lys Trp Ser Pro Ala Met Leu Trp Ala Leu Gly 
        115                 120                 125 

Phe Ile Phe Leu Phe Thr Val Gly Gly Leu Thr Gly Ile Val Leu Ser 
    130                 135                 140 

Asn Ser Ser Leu Asp Ile Val Leu His Asp Thr Tyr Tyr Val Val Ala 
145                 150                 155                 160 

His Phe His Tyr Val Leu Ser Met Gly Ala Val Phe Ala Ile Met Ala 
                165                 170                 175 

Cys Phe Val His Trp Phe Pro Leu Phe Ser Gly Tyr Thr Leu Asn Asp 
            180                 185                 190 

Thr Trp Ala Lys Ala His Phe Ala Ile Met Phe Val Gly Val Asn Met 
        195                 200                 205 

Thr Phe Phe Pro Gln His Phe Leu Gly Leu Ala Gly Met Pro Arg Arg 
    210                 215                 220 

Tyr Ser Asp Tyr Pro Asp Ala Tyr Thr Thr Trp Asn Thr Val Ser Ser 
225                 230                 235                 240 

Met Gly Ser Phe Ile Ser Leu Thr Ala Val Leu Val Met Ile Phe Met 
                245                 250                 255 

Ile Trp Glu Ala Phe Ala Ser Lys Arg Glu Val Leu Ser Ile Ser Tyr 
            260                 265                 270 

Ser Ser Thr Asn Leu Glu Trp Leu His Gly Cys Pro Pro Pro Tyr His 
        275                 280                 285 

Thr Phe Glu Glu Pro Ser Tyr Val Lys Val Lys 
    290                 295 

 
           
             31  
             987  
             DNA  
             Rattus norvegicus  
             
               Cytochrome C oxidase, subunit 1  
             
           
            31 

gaattccctt tcttcgaccc cgctggaggt ggagacccaa ttctttatca acacctattc     60 

tgattcttcg gccacccaga agtgtacatc ttaattcttc cagggtttgg aattatttca    120 

catgtagtta cctattactc tggaaaaaaa gaacccttcg gatatatagg tatggtatga    180 

gccataatat ctattggctt cctaggattt attgtatgag cacatcacat attcacagta    240 

ggcctagatg tagacacccg agcctacttt acatctgcca ctataattat cgcaattcct    300 

acaggcgtaa aagtattcag ctgactcgct acactacatg gaggaaatat caaatgatcc    360 

cccgccatat tatgagcctt agggtttatc ttcttattca cagtaggggg cctaacaggg    420 

atcgtactat ctaactcatc ccttgacatt gtacttcatg atacatacta cgtagtagct    480 

cacttccact atgtcttatc tataggagca gtattcgcca tcatagcttg cttcgtccac    540 

tgattcccac tattctcagg ctatacccta aatgacacat gagcaaaagc ccactttgcc    600 

attatatttg taggtgtaaa cataacattt tttcctcaac acttcctagg attagcaggg    660 

atacctcgtc gttactctga ttatccagat gcttatacca catgaaatac agtctcctct    720 

ataggctcat tcatctcact tacggccgtc cttgtaatga tcttcatgat ttgagaagcc    780 

ttcgcatcaa aacgagaagt gctctcaatt tcctactctt caactaacct agaatgactg    840 

catggatgcc ccccacctta ccacacattc gaagaacctt cctatgtaaa agttaaataa    900 

gaaaggaagg attcgaaccc cctacaactg gtttcaagcc aatttcataa ccattatgtc    960 

tttctcaata aaaaaaaaaa ggaattc                                        987 

 
           
             32  
             334  
             PRT  
             Rattus norvegicus  
             
               LDH-B  
             
           
            32 

Met Ala Thr Leu Lys Glu Lys Leu Ile Ala Pro Val Ala Asp Asp Glu 
1               5                   10                  15 

Thr Ala Val Pro Asn Asn Lys Ile Thr Val Val Gly Val Gly Gln Val 
            20                  25                  30 

Gly Met Ala Cys Ala Ile Ser Ile Leu Gly Lys Ser Leu Ala Asp Glu 
        35                  40                  45 

Leu Ala Leu Val Asp Val Leu Glu Asp Lys Leu Lys Gly Glu Met Met 
    50                  55                  60 

Asp Leu Gln His Gly Ser Leu Phe Leu Gln Thr Pro Lys Ile Val Ala 
65                  70                  75                  80 

Asp Lys Asp Tyr Ser Val Thr Ala Asn Ser Lys Ile Val Val Val Thr 
                85                  90                  95 

Ala Gly Val Arg Gln Gln Glu Gly Glu Ser Arg Leu Asn Leu Val Gln 
            100                 105                 110 

Arg Asn Val Asn Val Phe Lys Phe Ile Ile Pro Gln Ile Val Lys Tyr 
        115                 120                 125 

Ser Pro Asp Cys Thr Ile Ile Val Val Ser Asn Pro Val Asp Ile Leu 
    130                 135                 140 

Thr Tyr Val Thr Trp Lys Leu Ser Gly Leu Pro Lys His Arg Val Ile 
145                 150                 155                 160 

Gly Ser Gly Cys Asn Leu Asp Ser Ala Arg Phe Arg Tyr Leu Met Ala 
                165                 170                 175 

Glu Lys Leu Gly Ile His Pro Ser Ser Cys His Gly Trp Ile Leu Gly 
            180                 185                 190 

Glu His Gly Asp Ser Ser Val Ala Val Trp Ser Gly Val Asn Val Ala 
        195                 200                 205 

Gly Val Ser Leu Gln Glu Leu Asn Pro Glu Met Gly Thr Asp Asn Asp 
    210                 215                 220 

Ser Glu Asn Trp Lys Glu Val His Lys Met Val Val Asp Ser Ala Tyr 
225                 230                 235                 240 

Glu Val Ile Lys Leu Lys Gly Tyr Thr Asn Trp Ala Ile Gly Leu Ser 
                245                 250                 255 

Val Ala Asp Leu Ile Glu Ser Met Leu Lys Asn Leu Ser Arg Ile His 
            260                 265                 270 

Pro Val Ser Thr Met Val Lys Gly Met Tyr Gly Ile Glu Asn Glu Val 
        275                 280                 285 

Phe Leu Ser Leu Pro Cys Ile Leu Asn Ala Arg Gly Leu Thr Ser Val 
    290                 295                 300 

Ile Asn Gln Lys Leu Lys Asp Asp Glu Val Ala Gln Leu Arg Lys Ser 
305                 310                 315                 320 

Ala Asp Thr Leu Trp Asp Ile Gln Lys Asp Leu Lys Asp Leu 
                325                 330 

 
           
             33  
             1217  
             DNA  
             Rattus norvegicus  
             
               LDH-B  
             
           
            33 

cagagcctcc tcttgtctgg acaagatggc aacccttaag gaaaagctca ttgcgccagt     60 

cgcagacgac gagactgccg tcccgaacaa caagattact gtagtaggcg ttggacaagt    120 

tggaatggct tgtgctatca gcattctggg gaagtctctg gctgatgagc ttgccctggt    180 

ggatgtcttg gaagacaagc tcaaaggaga aatgatggat ctgcagcacg ggagcttatt    240 

tctccagact ccgaaaatcg tggctgataa agattactcc gtgacagcca attctaagat    300 

tgtggtggtg accgcgggag tccgccagca ggagggggag agtcggctca acctggtgca    360 

gagaaacgtc aatgtattca agttcattat tcctcagatc gtcaagtaca gccccgactg    420 

caccatcatc gtggtttcca acccagtgga tattcttacc tatgtcacct ggaagctgag    480 

cgggctacct aagcaccgcg tgattggaag tggatgcaat ctggattctg ctcggtttcg    540 

ttacctcatg gccgaaaagc ttggtattca tcccagcagc tgccacggct ggatcctggg    600 

cgagcacggg gactccagtg tggcagtgtg gagcggggtg aatgtggcag gagtctccct    660 

ccaggaactg aacccagaga tgggaacgga caatgacagc gagaactgga aggaggtgca    720 

taagatggtg gtggacagtg cctatgaagt catcaagcta aaaggctaca ccaactgggc    780 

catcggccta agtgtggctg acctcatcga atccatgctg aaaaacctct ctcggattca    840 

ccccgtgtct acaatggtga agggaatgta cggcatcgag aacgaagtct tcctcagtct    900 

cccgtgcatc cttaatgctc ggggactgac cagcgtcatc aaccagaagc tgaaggacga    960 

tgaggtcgct cagctcagga agagtgcgga caccctgtgg gatatccaga aagacctcaa   1020 

ggacctgtga ctgccaggcg ccaggctgta gaaatccaaa cctccaatgt gactaagtga   1080 

acctttagtc ttcggccttg tacgtaggtc acagtttgct tcttccctaa catgtgataa   1140 

tgagctcaca gatcaaaacc aggagtgttt gatgtttgca ctaggagctc ctgaacaaat   1200 

aaagtttagc aattgca                                                  1217 

 
           
             34  
             86  
             PRT  
             Homo sapiens  
             
               Cytochrome C oxidase subunit VIb  
             
           
            34 

Met Ala Glu Asp Met Glu Thr Lys Ile Lys Asn Tyr Lys Thr Ala Pro 
1               5                   10                  15 

Phe Asp Ser Arg Phe Pro Asn Gln Asn Gln Thr Arg Asn Cys Trp Gln 
            20                  25                  30 

Asn Tyr Leu Asp Phe His Arg Cys Gln Lys Ala Met Thr Ala Lys Gly 
        35                  40                  45 

Gly Asp Ile Ser Val Cys Glu Trp Tyr Gln Arg Val Tyr Gln Ser Leu 
    50                  55                  60 

Cys Pro Thr Ser Trp Val Thr Asp Trp Asp Glu Gln Arg Ala Glu Gly 
65                  70                  75                  80 

Thr Phe Pro Gly Lys Ile 
                85 

 
           
             35  
             439  
             DNA  
             Homo sapiens  
             
               Cytochrome C oxidase subunit VIb  
             
           
            35 

ttgagctgca ggttgaatcc ggggtgcctt taggattcag caccatggcg gaagacatgg     60 

agaccaaaat caagaactac aagaccgccc cttttgacag ccgcttcccc aaccagaacc    120 

agactagaaa ctgctggcag aactacctgg acttccaccg ctgtcagaag gcaatgaccg    180 

ctaaaggagg cgatatctct gtgtgcgaat ggtaccagcg tgtgtaccag tccctctgcc    240 

ccacatcctg ggtcacagac tgggatgagc aacgggctga aggcacgttt cccgggaaga    300 

tctgaactgg ctgcatctcc ctttcctctg tcctccatcc ttctcccagg atggtgaagg    360 

gggacctggt acccagtgat ccccacccca ggatcctaaa tcatgactta cctgctaata    420 

aaaactcatt ggaaaagtg                                                 439 

 
           
             36  
             172  
             PRT  
             Homo sapiens  
             
               NADH ubiquinone oxidoreductase PGIV subunit  
             
           
            36 

Met Pro Gly Ile Val Glu Leu Pro Thr Leu Glu Glu Leu Lys Val Asp 
1               5                   10                  15 

Glu Val Lys Ile Ser Ser Ala Val Leu Lys Ala Ala Ala His His Tyr 
            20                  25                  30 

Gly Ala Gln Cys Asp Lys Pro Asn Lys Glu Phe Met Leu Cys Arg Trp 
        35                  40                  45 

Glu Glu Lys Asp Pro Arg Arg Cys Leu Glu Glu Gly Lys Leu Val Asn 
    50                  55                  60 

Lys Cys Ala Leu Asp Phe Phe Arg Gln Ile Lys Arg His Cys Ala Glu 
65                  70                  75                  80 

Pro Phe Thr Glu Tyr Trp Thr Cys Ile Asp Tyr Thr Gly Gln Gln Leu 
                85                  90                  95 

Phe Arg His Cys Arg Lys Gln Gln Ala Lys Phe Asp Glu Cys Val Leu 
            100                 105                 110 

Asp Lys Leu Gly Trp Val Arg Pro Asp Leu Gly Glu Leu Ser Lys Val 
        115                 120                 125 

Thr Lys Val Lys Thr Asp Arg Pro Leu Pro Glu Asn Pro Tyr His Ser 
    130                 135                 140 

Arg Pro Arg Pro Asp Pro Ser Pro Glu Ile Glu Gly Asp Leu Gln Pro 
145                 150                 155                 160 

Ala Thr His Gly Ser Arg Phe Tyr Phe Trp Thr Lys 
                165                 170 

 
           
             37  
             700  
             DNA  
             Homo sapiens  
             
               NADH ubiquinone oxidoreductase PGIV subunit  
             
           
            37 

ggggagttca aggagacggg ggcgacgcgg ctgagggctt ctcgtcgggg tcggggctgc     60 

agccgtcatg ccggggatag tggagctgcc cactctagag gagctgaaag tagatgaggt    120 

gaaaattagt tctgctgtgc ttaaagctgc ggcccatcac tatggagctc aatgtgataa    180 

gcccaacaag gaatttatgc tctgccgctg ggaagagaaa gatccgaggc ggtgtttaga    240 

ggaaggcaaa ctggtcaaca agtgtgcttt ggacttcttt aggcagataa aacgtcactg    300 

tgcagagcct tttacagaat attggacttg cattgattat actggccagc agttatttcg    360 

tcactgtcgc aaacagcagg caaagtttga cgagtgtgtg ctggacaaac tgggctgggt    420 

gcggcctgac ctgggagaac tgtcaaaggt caccaaagtg aaaacagatc gacctttacc    480 

ggagaatccc tatcactcaa gaccaagacc ggatcccagc cctgagatcg agggagatct    540 

gcagcctgcc acacatggca gccgctttta tttctggacc aagtaaagat gggtccgtgg    600 

cccacactcg gtcatgtgct cagacaacga ctgatgaaaa cgcccatgcg gtttgcatcg    660 

actgatagtg tgttctttcc gggatcacaa acattaacaa                          700 

 
           
             38  
             532  
             PRT  
             Mus musculus  
             
               succinate dehydrogenase Fp subunit  
             
           
            38 

Leu Arg Ala Ala Phe Gly Leu Ser Glu Ala Gly Phe Asn Thr Ala Cys 
1               5                   10                  15 

Leu Thr Lys Leu Phe Pro Thr Arg Ser His Thr Val Ala Ala Gln Gly 
            20                  25                  30 

Gly Ile Asn Ala Ala Leu Gly Asn Met Glu Glu Asp Asn Trp Arg Trp 
        35                  40                  45 

His Phe Tyr Asp Thr Val Lys Gly Ser Asp Trp Leu Gly Asp Gln Asp 
    50                  55                  60 

Ala Ile His Tyr Met Thr Glu Gln Ala Pro Ala Ser Val Val Glu Leu 
65                  70                  75                  80 

Glu Asn Tyr Gly Met Pro Phe Ser Arg Thr Glu Asp Gly Lys Ile Tyr 
                85                  90                  95 

Gln Arg Ala Phe Gly Gly Gln Ser Leu Lys Phe Gly Lys Gly Gly Gln 
            100                 105                 110 

Ala His Arg Cys Cys Cys Val Ala Asp Arg Thr Gly His Ser Leu Leu 
        115                 120                 125 

His Thr Leu Tyr Gly Arg Ser Leu Arg Tyr Asp Thr Ser Tyr Phe Val 
    130                 135                 140 

Glu Tyr Phe Ala Leu Asp Leu Leu Met Glu Asn Gly Glu Cys Arg Gly 
145                 150                 155                 160 

Val Ile Ala Leu Cys Ile Glu Asp Gly Ser Ile His Arg Ile Arg Ala 
                165                 170                 175 

Lys Asn Thr Val Ile Ala Thr Gly Gly Tyr Gly Arg Thr Tyr Phe Ser 
            180                 185                 190 

Cys Thr Ser Ala His Thr Ser Thr Gly Asp Gly Thr Ala Met Val Thr 
        195                 200                 205 

Arg Ala Gly Leu Pro Cys Gln Asp Leu Glu Phe Val Gln Phe His Pro 
    210                 215                 220 

Thr Gly Ile Tyr Gly Ala Gly Cys Leu Ile Thr Glu Gly Cys Arg Gly 
225                 230                 235                 240 

Glu Gly Gly Ile Leu Ile Asn Ser Gln Gly Glu Arg Phe Met Glu Arg 
                245                 250                 255 

Tyr Ala Pro Val Ala Lys Asp Leu Ala Ser Arg Asp Val Val Ser Arg 
            260                 265                 270 

Ser Met Thr Leu Glu Ile Arg Glu Gly Arg Gly Cys Gly Pro Glu Lys 
        275                 280                 285 

Asp His Val Tyr Leu Gln Leu His His Leu Pro Pro Glu Gln Leu Ala 
    290                 295                 300 

Thr Arg Leu Pro Gly Ile Ser Glu Thr Ala Met Ile Phe Ala Gly Val 
305                 310                 315                 320 

Asp Val Thr Lys Glu Pro Ile Pro Val Leu Pro Thr Val His Tyr Asn 
                325                 330                 335 

Met Gly Gly Ile Pro Thr Asn Tyr Lys Gly Gln Val Leu Lys His Val 
            340                 345                 350 

Asn Gly Gln Asp Gln Ile Val Pro Gly Leu Tyr Ala Cys Gly Glu Ala 
        355                 360                 365 

Ala Cys Ala Ser Val His Gly Ala Asn Arg Leu Gly Ala Asn Ser Leu 
    370                 375                 380 

Leu Asp Leu Val Val Phe Gly Arg Ala Cys Ala Leu Ser Ile Ala Glu 
385                 390                 395                 400 

Ser Cys Arg Pro Gly Asp Lys Val Pro Ser Ile Lys Ala Asn Ala Gly 
                405                 410                 415 

Glu Glu Ser Val Met Asn Leu Asp Lys Leu Arg Phe Ala Asp Gly Ser 
            420                 425                 430 

Ile Arg Thr Ser Glu Leu Arg Leu Asn Met Gln Lys Ser Met Gln Asn 
        435                 440                 445 

His Ala Ala Val Phe Arg Val Gly Ser Val Leu Gln Glu Gly Cys Glu 
    450                 455                 460 

Lys Ile Ser Gln Leu Tyr Gly Asp Leu Lys His Leu Lys Thr Phe Asp 
465                 470                 475                 480 

Arg Gly Met Val Trp Asn Thr Asp Leu Val Glu Thr Leu Glu Leu Gln 
                485                 490                 495 

Asn Leu Met Leu Cys Ala Leu Gln Thr Ile Tyr Gly Ala Glu Ala Arg 
            500                 505                 510 

Lys Glu Ser Arg Gly Ala His Ala Arg Glu Asp Tyr Lys Val Arg Val 
        515                 520                 525 

Asp Glu Tyr Asp 
    530 

 
           
             39  
             1596  
             DNA  
             Mus musculus  
             
               succinate dehydrogenase Fp subunit  
             
           
            39 

ttgcgagctg catttggcct ttctgaggca gggtttaata ctgcatgcct tacaaagctc     60 

tttcctaccc gatcacatac tgttgcagca cagggaggta tcaatgctgc tctggggaac    120 

atggaagagg acaactggag atggcatttc tatgacactg tgaaaggctc cgactggctg    180 

ggggatcagg atgccatcca ttacatgaca gagcaagctc ctgcctctgt ggttgagcta    240 

gaaaattatg gtatgccatt tagcagaact gaagatggga agatttatca gcgtgcattt    300 

ggtggacaga gcctcaagtt tgggaaaggc gggcaggctc atcggtgttg ctgtgtggct    360 

gatcggacag gccactcact cttacacacc ttgtatggaa gatctctgcg atatgacacc    420 

agttattttg tggaatattt tgcactggat cttctgatgg aaaatgggga gtgccgtggt    480 

gtcattgcac tgtgcataga agatgggtcc atacaccgaa taagagcaaa aaacactgtt    540 

attgctactg ggggctacgg gcgaacctac ttcagctgta catctgccca taccagcaca    600 

ggggacggca cagccatggt cactagggct ggtttgcctt gccaggactt agaatttgtt    660 

cagttccacc ccacaggtat atacggtgct ggctgcctca tcacagaagg gtgtcgtgga    720 

gaggggggga ttctcatcaa cagtcaaggt gaaaggttca tggagagata cgcccctgtt    780 

gccaaggacc tggcatcaag agatgttgtg tctcgatcca tgactcttga gatccgtgaa    840 

ggaagaggct gtggccctga gaaagatcac gtctacctgc agttgcatca tctgcctcct    900 

gagcaactgg ccacacgtct gcccggaatt tcagagacag ccatgatctt cgctggtgtg    960 

gatgtcacta aggagcccat tccagtcctc cccactgtgc attacaacat gggtgggatt   1020 

cccactaact acaagggaca ggtgctgaag catgtgaatg gccaggatca gattgtgcct   1080 

ggactgtatg cctgtgggga ggctgcctgc gcctcagtgc atggtgccaa ccggcttgga   1140 

gcaaattctc tcttggacct tgtagtcttt ggcagagcct gtgccctgag cattgcagaa   1200 

tcttgcaggc ctggagataa agttccttca attaaggcaa atgctggaga agaatcggtt   1260 

atgaatcttg acaagttgag atttgccgat ggaagtataa gaacatcaga actacgccta   1320 

aacatgcaga agtcgatgca gaaccatgct gcagtgttcc gtgtggggag tgtattgcaa   1380 

gaaggctgtg aaaaaatcag tcagctctat ggagacctaa agcatctaaa gacattcgac   1440 

aggggaatgg tttggaacac agacctggtg gagaccctgg agctgcagaa tctgatgctg   1500 

tgcgcactgc agaccatata tggtgcagaa gctcggaagg agtcccgggg agcccatgcc   1560 

agggaagatt acaaagtgcg ggtcgatgag tatgat                             1596 

 
           
             40  
             453  
             PRT  
             Homo sapiens  
             
               Core protein II of human mitochondrial 
      cytochrome bc-1 complex  
             
           
            40 

Met Lys Leu Leu Thr Arg Ala Gly Ser Phe Ser Arg Phe Tyr Ser Leu 
1               5                   10                  15 

Lys Val Ala Pro Lys Val Lys Ala Thr Ala Ala Pro Ala Gly Ala Pro 
            20                  25                  30 

Pro Gln Pro Gln Asp Leu Glu Phe Thr Lys Leu Pro Asn Gly Leu Val 
        35                  40                  45 

Ile Ala Ser Leu Glu Asn Tyr Ser Pro Val Ser Arg Ile Gly Leu Phe 
    50                  55                  60 

Ile Lys Ala Gly Ser Arg Tyr Glu Asp Phe Ser Asn Leu Gly Thr Thr 
65                  70                  75                  80 

His Leu Leu Arg Leu Thr Ser Ser Leu Thr Thr Lys Gly Ala Ser Ser 
                85                  90                  95 

Phe Lys Ile Thr Arg Gly Ile Glu Ala Val Gly Gly Lys Leu Ser Val 
            100                 105                 110 

Thr Ala Thr Arg Glu Asn Met Ala Tyr Thr Val Glu Cys Leu Arg Gly 
        115                 120                 125 

Asp Val Asp Ile Leu Met Glu Phe Leu Leu Asn Val Thr Thr Ala Pro 
    130                 135                 140 

Glu Phe Arg Arg Trp Glu Val Ala Asp Leu Gln Pro Gln Leu Lys Ile 
145                 150                 155                 160 

Asp Lys Ala Val Ala Phe Gln Asn Pro Gln Thr His Val Ile Glu Asn 
                165                 170                 175 

Leu His Ala Ala Ala Tyr Gln Asn Ala Leu Ala Asn Pro Leu Tyr Cys 
            180                 185                 190 

Pro Asp Tyr Arg Ile Gly Lys Val Thr Ser Glu Glu Leu His Tyr Phe 
        195                 200                 205 

Val Gln Asn His Phe Thr Ser Ala Arg Met Ala Leu Ile Gly Leu Gly 
    210                 215                 220 

Val Ser His Pro Val Leu Lys Gln Val Ala Glu Gln Phe Leu Asn Met 
225                 230                 235                 240 

Arg Gly Gly Leu Gly Leu Ser Gly Ala Lys Ala Asn Tyr Arg Gly Gly 
                245                 250                 255 

Glu Ile Arg Glu Gln Asn Gly Asp Ser Leu Val His Ala Ala Phe Val 
            260                 265                 270 

Ala Glu Ser Ala Val Ala Gly Ser Ala Glu Ala Asn Ala Phe Ser Val 
        275                 280                 285 

Leu Gln His Val Leu Gly Ala Gly Pro His Val Lys Arg Gly Ser Asn 
    290                 295                 300 

Thr Thr Ser His Leu His Gln Ala Val Ala Lys Ala Thr Gln Gln Pro 
305                 310                 315                 320 

Phe Asp Val Ser Ala Phe Asn Ala Ser Tyr Ser Asp Ser Gly Leu Phe 
                325                 330                 335 

Gly Ile Tyr Thr Ile Ser Gln Ala Thr Ala Ala Gly Asp Val Ile Lys 
            340                 345                 350 

Ala Ala Tyr Asn Gln Val Lys Arg Ile Ala Gln Gly Asn Leu Ser Asn 
        355                 360                 365 

Thr Asp Val Gln Ala Ala Lys Asn Lys Leu Lys Ala Gly Tyr Leu Met 
    370                 375                 380 

Ser Val Glu Ser Ser Glu Cys Phe Leu Glu Glu Val Gly Ser Gln Ala 
385                 390                 395                 400 

Leu Val Ala Gly Ser Tyr Met Pro Pro Ser Thr Val Leu Gln Gln Ile 
                405                 410                 415 

Asp Ser Val Ala Asn Ala Asp Ile Ile Asn Ala Ala Lys Lys Phe Val 
            420                 425                 430 

Ser Gly Gln Lys Ser Met Ala Ala Ser Gly Asn Leu Gly His Thr Pro 
        435                 440                 445 

Phe Val Asp Glu Leu 
    450 

 
           
             41  
             1588  
             DNA  
             Homo sapiens  
             
               Core protein II of human mitochondrial 
      cytochrome bc-1 complex  
             
           
            41 

atcttgcttt cctttaatcc ggcagtgacc gtgtgtcaga acaatcttga atcatgaagc     60 

tactaaccag agccggctct ttctcgagat tttattccct caaagttgcc cccaaagtta    120 

aagccacagc tgcgcctgca ggagcaccgc cacaacctca ggaccttgag tttaccaagt    180 

taccaaatgg cttggtgatt gcttctttgg aaaactattc tcctgtatca agaattggtt    240 

tgttcattaa agcaggcagt agatatgagg acttcagcaa tttaggaacc acccatttgc    300 

tgcgtcttac atccagtctg acgacaaaag gagcttcatc tttcaagata acccgtggaa    360 

ttgaagcagt tggtggcaaa ttaagtgtga ccgcaacaag ggaaaacatg gcttatactg    420 

tggaatgcct gcggggtgat gttgatattc taatggagtt cctgctcaat gtcaccacag    480 

caccagaatt tcgtcgttgg gaagtagctg accttcagcc tcagctaaag attgacaaag    540 

ctgtggcctt tcagaatccg cagactcatg tcattgaaaa tttgcatgca gcagcttacc    600 

agaatgcctt ggctaatccc ttgtattgtc ctgactatag gattggaaaa gtgacatcag    660 

aggagttaca ttacttcgtt cagaaccatt tcacaagtgc aagaatggct ttgattggac    720 

ttggtgtgag tcatcctgtt ctaaagcaag ttgctgaaca gtttctcaac atgaggggtg    780 

ggcttggttt atctggtgca aaggccaact accgtggagg tgaaatccga gaacagaatg    840 

gagacagtct tgtccatgct gcttttgtag cagaaagtgc tgtcgcggga agtgcagagg    900 

caaatgcatt tagtgttctt cagcatgtcc tcggtgctgg gccacatgtc aagaggggca    960 

gcaacaccac cagccatctg caccaggctg ttgccaaggc aactcagcag ccatttgatg   1020 

tttctgcatt taatgccagt tactcagatt ctggactctt tgggatttat actatctccc   1080 

aggccacagc tgctggagat gttatcaagg ctgcctataa tcaagtaaaa agaatagctc   1140 

aaggaaacct ttccaacaca gatgtccaag ctgccaagaa caagctgaaa gctggatacc   1200 

taatgtcagt ggagtcttct gagtgtttcc tggaagaagt cgggtcccag gctctagttg   1260 

ctggttctta catgccacca tccacagtcc ttcagcagat tgattcagtg gctaatgctg   1320 

atatcataaa tgcggcaaag aagtttgttt ctggccagaa gtcaatggca gcaagtggaa   1380 

atttgggaca tacacctttt gttgatgagt tgtaatactg atgcacacat tacaggagag   1440 

agctgaacgt tctctcaccc agagcagcaa acacatgaaa gtcagaagtc tctaatatat   1500 

catttgtctt ttttccagtg aggtaaaata aggcataaat gcaggtaatt attcccagct   1560 

gacctaaagt caataaaaca ttctgttt                                      1588 

 
           
             42  
             358  
             PRT  
             Rattus norvegicus  
             
               Stearoyl-CoA desaturase-2  
             
           
            42 

Met Pro Ala His Ile Leu Gln Glu Ile Ser Gly Ser Tyr Ser Ala Thr 
1               5                   10                  15 

Thr Thr Ile Thr Ala Pro Pro Ser Gly Gly Gln Gln Asn Gly Gly Glu 
            20                  25                  30 

Lys Phe Glu Lys Asn Pro His His Trp Gly Ala Asp Val Arg Pro Glu 
        35                  40                  45 

Ile Lys Asp Asp Leu Tyr Asp Pro Ser Tyr Gln Asp Glu Glu Gly Pro 
    50                  55                  60 

Pro Pro Lys Leu Glu Tyr Val Trp Arg Asn Ile Ile Leu Met Ala Leu 
65                  70                  75                  80 

Leu His Ile Gly Ala Leu Tyr Gly Ile Thr Leu Val Pro Ser Cys Lys 
                85                  90                  95 

Val Tyr Thr Cys Leu Phe Ala Tyr Leu Tyr Tyr Val Ile Ser Ala Leu 
            100                 105                 110 

Gly Ile Thr Ala Gly Ala His Arg Leu Trp Ser His Arg Thr Tyr Lys 
        115                 120                 125 

Ala Arg Leu Pro Leu Arg Leu Phe Leu Ile Ile Ala Asn Thr Met Ala 
    130                 135                 140 

Phe Gln Asn Asp Val Tyr Glu Trp Ala Arg Asp His Arg Ala His His 
145                 150                 155                 160 

Lys Phe Ser Glu Thr His Ala Asp Pro His Asn Ser Arg Arg Gly Phe 
                165                 170                 175 

Phe Phe Ser His Val Gly Trp Leu Leu Val Arg Lys His Pro Ala Val 
            180                 185                 190 

Lys Glu Lys Gly Gly Lys Leu Asp Met Ser Asp Leu Lys Ala Glu Lys 
        195                 200                 205 

Leu Val Met Phe Gln Arg Arg Tyr Tyr Lys Pro Gly Leu Leu Leu Met 
    210                 215                 220 

Cys Phe Ile Leu Pro Thr Leu Val Pro Trp Tyr Cys Trp Gly Glu Thr 
225                 230                 235                 240 

Phe Val Asn Ser Leu Cys Val Ser Thr Phe Leu Arg Tyr Ala Val Val 
                245                 250                 255 

Leu Asn Ala Thr Trp Leu Val Asn Ser Ala Ala His Leu Tyr Gly Tyr 
            260                 265                 270 

Arg Pro Tyr Asp Lys Asn Ile Ser Ser Arg Glu Asn Ile Leu Val Ser 
        275                 280                 285 

Met Gly Ala Val Gly Glu Gly Phe His Asn Tyr His His Ala Phe Pro 
    290                 295                 300 

Tyr Asp Tyr Ser Ala Ser Glu Tyr Arg Trp His Ile Asn Phe Thr Thr 
305                 310                 315                 320 

Phe Phe Ile Asp Cys Met Ala Leu Leu Gly Leu Ala Tyr Asp Arg Lys 
                325                 330                 335 

Arg Val Ser Lys Ala Ala Val Leu Ala Arg Ile Lys Arg Thr Gly Glu 
            340                 345                 350 

Glu Ser Cys Lys Ser Gly 
        355 

 
           
             43  
             5055  
             DNA  
             Rattus norvegicus  
             
               Stearoyl-CoA desaturase-2  
             
           
            43 

ctgggctagg gatgggcaca atgcggtgtt ggtgtctgca gtattctact cctggacacc     60 

ggtggcctgc aagtgcgctt ttggcgtcct ctcatttttc tatccttatc tccgtccgcg    120 

gctgccttgg ccagccagtt tttgattttt tatctgttcc ttgatcgata caaagaacct    180 

aaggacatac aggacattaa tacccaactg ccagctctgg cccagggctt gtactgtgca    240 

gcgggctggc tgcagaaact taagtcataa cactctttgt cgctgaggtc tgaagctctc    300 

tgcacgttct catccctggg aacgtgaccc cagcatccga cgccaagatg ccggctcaca    360 

tactgcaaga gatctctggc tcttactcgg ccaccaccac aatcacagcg ccaccttctg    420 

ggggacagca gaatggagga gagaagtttg aaaagaatcc tcaccactgg ggagcagatg    480 

ttcgccctga aattaaagat gacctatacg accccagcta ccaggatgag gaggggcccc    540 

cgcccaagct ggagtacgtc tggaggaaca tcatcctcat ggccctgctg cacattggag    600 

ccctgtacgg gatcacactg gtcccctcct gcaaggtcta cacctgcctc tttgcatatt    660 

tgtactacgt aatcagcgcc ctgggcatca cagccggggc tcatcgcctg tggagccaca    720 

ggacttacaa ggctcggctg cccctgaggc tctttctcat catcgccaac accatggcat    780 

tccagaacga tgtgtacgaa tgggcccggg atcaccgcgc ccaccacaag ttctcagaga    840 

cacacgccga ccctcacaac tcccgccgtg gctttttctt ctctcacgtg ggttggctgc    900 

ttgtgcgcaa acacccggct gtcaaagaga agggcggaaa gctggacatg tctgacctga    960 

aagctgagaa gctggtgatg ttccagagga ggtattacaa gcccggcctc ctgctcatgt   1020 

gcttcatcct gcccacgctg gtgccctggt actgctgggg tgagacgttt gtaaacagcc   1080 

tgtgcgtcag cactttctta cggtacgctg tggtgctcaa cgccacctgg ctggtgaaca   1140 

gcgccgccca cctctatgga tatcgcccct acgacaagaa catcagctct cgggagaaca   1200 

tcctggtgtc gatgggagct gtgggcgagg gcttccacaa ctaccatcac gccttcccct   1260 

atgactactc tgccagtgag taccgctggc acatcaactt caccacgttc ttcatcgact   1320 

gcatggccct cctgggcctg gcttatgacc ggaagagggt gtccaaggcc gctgtcttag   1380 

ccaggattaa gagaactggg gaggagagct gcaagagtgg ctgagttcgg ggtcttccag   1440 

ttcctgttcc aagagccggc ttggcagagg cttactgttc tgttaattaa ctactgagta   1500 

ttgctaccca gatgctaaaa tgatgaagtt aacccattct ggtacagtat tgtaaaacgg   1560 

acaaatatcg gaagtcaaca gctctacctt ttgatgctaa gctgttctcc ccttctatct   1620 

tcttttcccg ttgtcttttt cttcgctttg tccccaatga cctcacccct catcgcctcc   1680 

cctaagccag cagccactcc gctgtagttc agagttcacc ttccagagcg taccagcttt   1740 

tcaagggtct acagtaaagg ttcacgcccc agccccaact taacgttgct ccaagttaca   1800 

gatagaagaa aaatcttgag aaatttctcc tgttatttta gtccagactt tgccagatgg   1860 

aatgggagaa aaaatagttt tatttggcag agagcttgcg aagtgggtaa atcttaaggg   1920 

gccaaattca acactcgggc tgtgattcat caaaattgtg ggagaggggg atctcctgtg   1980 

atcaggctcc gctccgtctg gccgaggtgc tggcgtctct tgacacagcg tgcctcctct   2040 

cctaattctg attgggtgac agccgtggaa ctttgtgaca ctaaaatatg gcaacacatc   2100 

ggtatctcag tgtactttag gctaaagatt taaaaaaata gagaagtgaa aaacaatttg   2160 

taggaaaagt ggtctttcct aaaataagat tttcttttcc tttcataaca aggacctggg   2220 

gccaggcgtc gtggcacccg cctcaaaaca gttgagagga gaggggcagg tgggtctctg   2280 

agtttgaagc cagccagggc tagatagtga ggccttgtct caaatagaaa agccaacaat   2340 

tagcaggaca cacggagccg ggggtcctct tgcgaggcgt gtctggaatc catgagggcg   2400 

gtcgctcgtg gtttttgagc ttcctgtgag ctgcaccccc agacacttgt aattcctgtt   2460 

cttccgtctt tcggatgcag gttgctccca caaattcagg atagctatgg cgtttccatg   2520 

catcccacaa tggaaggagt gtaagacttg agcttgagtc acagctaaaa cccagaagac   2580 

acaaagtcag tctgtcctgg atcctacaaa cactcaacga gtgcctccct ctggaaaaga   2640 

tggtgaacac cttccttcca ctgttgggca gtggacaggg aagttggagg tcgctgtagg   2700 

cagctccaag gtttctaggg gcaccttgcc aggggacctc acaactttgg cggtctctag   2760 

tggagcaggt taggttttag ctccctttgg ttcccgctgt gctttgtggt aaggcttttc   2820 

cctgggctgt tgcctcattc ctcactagct ctgtgaccag gtaggactct gggatacatg   2880 

gctgaaagct cttttgctct accacatctg cctctctttc cctacccatt tttttgtgtt   2940 

tcggggtggt atttttgtgg tggttgtttt gttttatttc ttctttcctc tttgacgttt   3000 

agtcttgctc taggcagaaa cgtcctggat atttacggtc agtaaagatg tccggtccca   3060 

gcagagatac aacctctatc tgcagtaggt agagaaggct gtccctttcc ggtggctttc   3120 

agattctcca cctctatccc tcccaggcct ctgatattct ttaaacttta aaggaaaagt   3180 

ttggccgact ttctagaaac atccctggct cagagaggct ctctgtctcc tcggttgttc   3240 

taaaacaaat ggtccgggtg gtgtgggtgc tggcccaaga ctcgattcct ctgagttagg   3300 

gcagagcatg ctccctgcac tcagcaaagc ctctcgggct gatcagtgct cctgggtgat   3360 

gctgtggtgc ttttaggagg aagacagtgt tggctggctg gaaagcatga cagttaaggt   3420 

tgtgttctta agtgcctcag tttgaaagct ttggctaggg taggagatct gagatgccgt   3480 

ggactttgca gaagagattt tgtgcagcaa cgcagaagcc tggctgtgtt aagattggct   3540 

gtacaggata gcgaatgctg tggttggagg gcacagtctt cccgtgttag ttaaatagag   3600 

aggctttagg acgtctctgc gcccacttga aggtagcctt atcctttagc tgttcgctaa   3660 

aatagaatcc ttgagagatt gacaatatat ttgctggcct gtcgccctat tccttggaga   3720 

ggccttttga atggttttaa ttccactggt tatccggtgc ccacttaatc aaatgtgaaa   3780 

aataaaaagc agctctcttc ttagcctgcc cccgtggatc tgaggaccct gttagataca   3840 

gctgtcggta agggtgtacc agctcactaa gaaacactac tgtcaagatt ttaaagctca   3900 

gaccaagtga cacgttaata agaaactaag atctgattga attttctaac agtccttgcc   3960 

tcgtggacac gctaaaattc gacttgggtg ccttacctct tttctaacta gtgttactca   4020 

tgggcctgcg ctcccttcct ctgcggattc cttagaaccg gagagcctgt aagtgtgaca   4080 

ggctcaaaac tggcctggct ggagggttag gtggactttg atctgcagtg ttccctctgg   4140 

ggtcttcgtt tggggaatat ttcttggggc tgttttgctg aagtgcccgt agctgatgga   4200 

tggtggaagt aatttgaacg tacgcgattt gtaggttttg ttatttgttt taagatttaa   4260 

aggctggttg tagcatttaa aagtgaaagc tttttcttcc tggtttgcta gtgttctgcg   4320 

tgtcttcttt agttgaaata ggttagtgga gaagttaaga aagcggtggc cgtgttacgc   4380 

tggtggtgaa ggccagggcc gcctacaaac tctcagtttt gttttgtttt gtttttttat   4440 

aacagtccca ctctgacaat aatgagttca ctccttggat aaggttgtta atgagggaag   4500 

agcccttcag acctaatcag ctcttactgg acccagctgt caacactgtt gcattgggga   4560 

ttaagtttcc aaccccgtga atcatgggac agactcaaac cgtagcactg ggcatgtcgc   4620 

ctaacaatat aatgtgactc agtattctgt gagacatggg ataatattcc tgacctacct   4680 

ccacgggtag gtgcgagcat gagcaaatgc tctttataaa acatttgggg atcctcagca   4740 

gaggagtatt taagtcctgc acatttttat agtaaccgtc caccatggac ttgacacgtg   4800 

cctatgtccc agacgacatt gttaatctac atcattcttc tcagaggttg aatggatgca   4860 

ttagataatg tatactcatt gctatggaaa cctgcaggac tccttttcct tccttgggtg   4920 

ggattttttt ttcctttatt atgtgcaaca gtagctattt gtaatctaat aattttcatt   4980 

aataccaact ctgaaagtat ttctactcat ctgaggttgt gtttgttcat aataaaatga   5040 

aatggatctg actgc                                                    5055 

 
           
             44  
             605  
             PRT  
             Rattus norvegicus  
             
               Ribophorin 1  
             
           
            44 

Met Glu Ala Pro Ile Val Leu Leu Leu Leu Leu Trp Leu Ala Leu Ala 
1               5                   10                  15 

Pro Thr Pro Gly Ser Ala Ser Ser Glu Ala Pro Pro Leu Val Asn Glu 
            20                  25                  30 

Asp Val Lys Arg Thr Val Asp Leu Ser Ser His Leu Ala Lys Val Thr 
        35                  40                  45 

Ala Glu Val Val Leu Ala His Pro Gly Gly Gly Ser Thr Ala Arg Ala 
    50                  55                  60 

Ser Ser Phe Val Leu Ala Leu Glu Pro Glu Leu Glu Ser Arg Leu Ala 
65                  70                  75                  80 

His Leu Gly Val Gln Val Lys Gly Glu Asp Glu Glu Asp Asn Asn Leu 
                85                  90                  95 

Glu Val Arg Glu Thr Lys Met Lys Gly Lys Ser Gly Arg Phe Phe Thr 
            100                 105                 110 

Val Lys Leu Pro Val Ala Leu Asp Pro Gly Ser Lys Ile Ser Ile Val 
        115                 120                 125 

Val Glu Thr Val Tyr Thr His Val Leu His Pro Tyr Pro Thr Gln Ile 
    130                 135                 140 

Thr Gln Ser Glu Lys Gln Phe Val Val Phe Glu Gly Asn His Tyr Phe 
145                 150                 155                 160 

Tyr Ser Pro Tyr Pro Thr Lys Thr Gln Thr Met Arg Val Arg Leu Ala 
                165                 170                 175 

Ser Arg Asn Val Glu Ser His Thr Lys Leu Gly Asn Pro Ser Arg Ser 
            180                 185                 190 

Glu Asp Ile Leu Asp Tyr Gly Pro Phe Lys Asp Ile Pro Ala Tyr Ser 
        195                 200                 205 

Gln Asp Thr Phe Lys Val His Tyr Glu Asn Asn Ser Pro Phe Leu Thr 
    210                 215                 220 

Ile Thr Ser Met Thr Arg Val Ile Glu Val Ser His Trp Gly Asn Ile 
225                 230                 235                 240 

Ala Val Glu Glu Asn Val Asp Leu Lys His Thr Gly Ala Val Leu Lys 
                245                 250                 255 

Gly Pro Phe Ser Arg Tyr Asp Tyr Gln Arg Gln Pro Asp Ser Gly Ile 
            260                 265                 270 

Ser Ser Ile Arg Ser Phe Lys Thr Ile Leu Pro Ala Ala Ala Gln Asp 
        275                 280                 285 

Val Tyr Tyr Arg Asp Glu Ile Gly Asn Val Ser Thr Ser His Leu Leu 
    290                 295                 300 

Ile Leu Asp Asp Ser Val Glu Met Glu Ile Arg Pro Arg Phe Gly Leu 
305                 310                 315                 320 

Phe Gly Gly Trp Lys Thr His Tyr Ile Val Gly Tyr Asn Leu Pro Ser 
                325                 330                 335 

Tyr Glu Tyr Leu Tyr Asn Leu Gly Asp Gln Tyr Ala Leu Lys Met Arg 
            340                 345                 350 

Phe Val Asp His Val Phe Asp Glu Gln Val Ile Asp Ser Leu Thr Val 
        355                 360                 365 

Lys Ile Ile Leu Pro Glu Gly Ala Lys Asn Ile Gln Val Asp Ser Pro 
    370                 375                 380 

Tyr Asp Ile Ser Arg Ala Pro Asp Glu Leu His Tyr Thr Tyr Leu Asp 
385                 390                 395                 400 

Thr Phe Gly Arg Pro Val Ile Val Ala Tyr Lys Lys Asn Leu Val Glu 
                405                 410                 415 

Gln His Ile Gln Asp Ile Val Val His Tyr Thr Phe Asn Lys Val Leu 
            420                 425                 430 

Met Leu Gln Glu Pro Leu Leu Val Val Ala Ala Phe Tyr Ile Leu Phe 
        435                 440                 445 

Phe Thr Val Ile Ile Tyr Val Arg Leu Asp Phe Ser Ile Thr Lys Asp 
    450                 455                 460 

Pro Ala Ala Glu Ala Arg Met Lys Val Ala Cys Ile Thr Glu Gln Val 
465                 470                 475                 480 

Leu Thr Leu Val Asn Lys Arg Leu Gly Leu Tyr Arg His Phe Asp Glu 
                485                 490                 495 

Thr Val Asn Arg Tyr Lys Gln Ser Arg Asp Ile Ser Thr Leu Asn Ser 
            500                 505                 510 

Gly Lys Lys Ser Leu Glu Thr Glu His Lys Ala Val Thr Ser Glu Ile 
        515                 520                 525 

Ala Val Leu Gln Ser Arg Leu Lys Thr Glu Gly Ser Asp Leu Cys Asp 
    530                 535                 540 

Arg Val Ser Glu Met Gln Lys Leu Asp Ala Gln Val Lys Glu Leu Val 
545                 550                 555                 560 

Leu Lys Ser Ala Val Glu Ala Glu Arg Leu Val Ala Gly Lys Leu Lys 
                565                 570                 575 

Lys Asp Thr Tyr Ile Glu Asn Glu Lys Leu Ser Ser Gly Lys Arg Gln 
            580                 585                 590 

Glu Leu Val Thr Lys Ile Asp His Ile Leu Asp Ala Leu 
        595                 600                 605  
           
             45  
             2214  
             DNA  
             Rattus norvegicus  
             
               Ribophorin 1  
             
           
            45 

tcgcggtcat ggaggcgccg atcgtcttgc tgctgctgct atggctcgcc ttggccccga     60 

cgcctggcag cgcctcttcg gaggctccgc cgctggtcaa cgaggacgtg aagcgcacgg    120 

tggacctgag cagccaccta gccaaggtga cggctgaggt ggtcctggcg cacccgggcg    180 

gcggctctac agcacgagcc agctctttcg ttctggccct ggagcccgaa ctggagtcgc    240 

gacttgcaca cctaggcgtg caggtaaagg gagaagatga ggaagacaac aacctagaag    300 

tacgagaaac caaaatgaag gggaaaagtg ggaggttttt caccgtcaag ctcccagttg    360 

ctcttgatcc tgggtccaag atctcaatcg ttgtggaaac tgtctacacc catgtgcttc    420 

atccataccc gactcagata actcagtcag agaaacagtt tgtggtgttt gagggcaacc    480 

attacttcta ctctccctat ccaacaaaga cccagaccat gcgagtgaga cttgcttccc    540 

gaaatgtgga gagccatacc aagctgggga acccctcaag gtctgaggac atcctggact    600 

atgggccttt taaagacatc cctgcctaca gtcaggatac tttcaaagta cattatgaga    660 

acaatagccc tttcctgacc atcaccagta tgacccgggt cattgaggtt tctcactggg    720 

gcaatattgc tgtggaagag aacgtggact tgaagcatac aggtgcggtg ctgaagggac    780 

ctttctcccg ctacgattac cagaggcagc ctgacagtgg gatctcctcc attcgttctt    840 

ttaagaccat ccttcctgct gctgcccagg atgtgtatta ccgggatgaa atcggtaatg    900 

tttccaccag ccacctcctt attttggatg actccgtgga aatggaaatc cggcctcgat    960 

ttggtctctt tggagggtgg aagacacact acatcgttgg ttacaacctc ccaagctatg   1020 

agtacctcta taatctgggt gaccagtatg cactgaagat gcggtttgta gaccacgtgt   1080 

ttgatgagca agtgatagat tctctgacag tgaagatcat ccttcctgag ggagccaaga   1140 

acatccaggt agacagtccc tacgatatta gccgggcccc agatgagctg cattacacct   1200 

acctagacac attcggccgc ccggtgattg ttgcttacaa gaagaatcta gttgaacagc   1260 

acattcagga cattgtggtg cactacacat tcaacaaggt gctcatgctg caggagcctc   1320 

tgctggttgt ggccgccttc tacatcctgt tcttcaccgt catcatctac gtccgtctag   1380 

acttttccat caccaaggac ccagcagcag aagccaggat gaaagtggcc tgtatcacag   1440 

agcaggtctt aaccctggtc aacaagaggt taggcctcta ccgtcacttt gatgagactg   1500 

tcaatagata caagcagtcc cgggacatct ctaccctcaa tagtggcaag aagagcctag   1560 

agacagagca caaagctgtg accagtgaga ttgctgtgct gcagtctagg ctgaagacgg   1620 

agggctctga cctgtgtgac agggtgagcg aaatgcagaa gctggacgcg caggtcaagg   1680 

agctggtact gaagtctgcg gtggaggcag agaggctggt ggctggcaag ctcaagaagg   1740 

acacatacat cgagaatgaa aagctcagct caggaaaacg ccaggagctg gtcaccaaga   1800 

tcgaccacat cctagacgct ctgtagctct tgtctccaga gcaggcctgg gtggcctaca   1860 

cttctggatg gaataggtag agggaggaag ttgtgattag cctaagacct caagaaaaaa   1920 

gctgaggccc atccatggct gtgtaagaag cttgtccctt atttctgaat ggttctcttt   1980 

tttacaaaaa caaaaaccta aacaaaaatc ctgcccaaaa aacaaacttg gttgtgtttt   2040 

gagcaaaagc attttgagta gttgattgct gttttgtttt gatacctggg tattttttct   2100 

gtctgtaagc cctgttttat ggccttctga ttcctaatgt ttggttcttt tgtgagcgta   2160 

tgcatggttt tttaaaagtg tgtatgacca aataaaaata aaggagggac tgtg         2214 

 
           
             46  
             284  
             PRT  
             Rattus norvegicus  
             
               Sulfotransferase-like protein  
             
           
            46 

Met Ala Glu Ser Glu Ala Glu Thr Pro Gly Thr Pro Gly Glu Phe Glu 
1               5                   10                  15 

Ser Lys Tyr Phe Glu Phe His Gly Val Arg Leu Pro Pro Phe Cys Arg 
            20                  25                  30 

Gly Lys Met Glu Asp Ile Ala Asp Phe Pro Val Arg Pro Ser Asp Val 
        35                  40                  45 

Trp Ile Val Thr Tyr Pro Lys Ser Gly Thr Ser Leu Leu Gln Glu Val 
    50                  55                  60 

Val Tyr Leu Val Ser Gln Gly Ala Asp Pro Asp Glu Ile Gly Leu Met 
65                  70                  75                  80 

Asn Ile Asp Glu Gln Leu Pro Val Leu Glu Tyr Pro Gln Pro Gly Leu 
                85                  90                  95 

Asp Ile Ile Lys Glu Leu Thr Ser Pro Arg Leu Ile Lys Ser His Leu 
            100                 105                 110 

Pro Tyr Arg Phe Leu Pro Ser Asp Leu His Asn Gly Asp Ser Lys Val 
        115                 120                 125 

Ile Tyr Met Ala Arg Asn Pro Lys Asp Leu Val Val Ser Tyr Tyr Gln 
    130                 135                 140 

Phe His Arg Ser Leu Arg Thr Met Ser Tyr Arg Gly Thr Phe Gln Glu 
145                 150                 155                 160 

Phe Cys Arg Arg Phe Met Asn Asp Lys Leu Gly Tyr Gly Ser Trp Phe 
                165                 170                 175 

Glu His Val Gln Glu Phe Trp Glu His Arg Met Asp Ala Asn Val Leu 
            180                 185                 190 

Phe Leu Lys Tyr Glu Asp Met His Arg Asp Leu Val Thr Met Val Glu 
        195                 200                 205 

Gln Leu Ala Arg Phe Leu Gly Val Ser Cys Asp Lys Ala Gln Leu Glu 
    210                 215                 220 

Ser Leu Ile Glu His Cys His Gln Leu Val Asp Gln Cys Cys Asn Ala 
225                 230                 235                 240 

Glu Ala Leu Pro Val Gly Arg Gly Arg Val Gly Leu Trp Lys Asp Ile 
                245                 250                 255 

Phe Thr Val Ser Met Asn Glu Lys Phe Asp Leu Val Tyr Lys Gln Lys 
            260                 265                 270 

Met Gly Lys Cys Asp Leu Thr Phe Asp Phe Tyr Leu 
        275                 280 

 
           
             47  
             2239  
             DNA  
             Rattus norvegicus  
             
               Sulfotransferase-like protein  
             
           
            47 

ggcacgagcc ctggagctgc gggccgggcc gggcggcagc atggcggaga gcgaagcgga     60 

gaccccgggc accccgggcg agttcgagag caagtacttc gagttccatg gcgtgcggct    120 

gcctcccttc tgccgcggga agatggagga catcgccgac ttcccagtgc ggcccagcga    180 

tgtgtggatc gtcacctacc ccaagtcagg tacgagcttg ctgcaagagg tggtctactt    240 

ggtgagccag ggggctgacc ctgatgagat tggcctcatg aacattgacg agcagctgcc    300 

agtactggag tacccacagc ccgggctgga catcatcaag gaactgacgt ctccccgcct    360 

catcaagagc caccttccct accgcttcct gccctctgac ctccacaacg gggactccaa    420 

ggtcatctac atggctcgca acccaaagga cctggtggta tcttactatc agttccaccg    480 

ctcgctgcgg accatgagtt accgaggaac cttccaggag ttctgtcgga ggttcatgaa    540 

cgacaaattg ggctatggct cctggtttga gcacgttcag gaattctggg aacatcgaat    600 

ggacgcaaat gtgcttttcc tcaagtatga agatatgcac cgggacctgg tgaccatggt    660 

ggagcagctg gccagattcc tgggtgtgtc ctgtgataag gcccagctgg agtccttgat    720 

cgagcactgc caccagctgg tggaccagtg ctgcaatgct gaggccctgc ctgtgggccg    780 

gggaagagtc gggctgtgga aggacatctt cactgtctcc atgaacgaga agtttgatct    840 

agtgtataaa cagaagatgg ggaagtgtga cctcacgttt gacttttatt tataatacca    900 

gaatccgcaa acttgcatgc tcacggtccc cagacgctct actagctaaa agtcctgttt    960 

gcgttcattt attccttgct ggacactccg gaagttgctt ggaaacagca gggggcgagc   1020 

ctggtggaga gagccgagga gtgagatgat gctgtctaca gcagccgcct ggccgtagcg   1080 

tttgagtctc cacgtgctgg ccataccaca cgcacgggca ggcaaatgtc tgcccctgta   1140 

ccccatgtta ttcactgtat tttatagcgc ttttcactga aaatcgaaat aaatatgtca   1200 

accaaatgca agtccatttc caagggggag caggagcgag ctgtgccggg atgctaggaa   1260 

gctctcaggg gtcactgtaa tttttatagt cttctctcta ggcacagcca tcccaccttg   1320 

tcccgagatc aaaggacagg ttggggggag ctggagaggt gggtgtagcc tgtggagttc   1380 

agttttctgg aaccatcctg acaccaagcc aggtcctgtc ccaggtctct gagcccagta   1440 

cagaatgttc tggaagggaa gctggcctga ggctgcggat ggcatctgaa ggcatcagtg   1500 

gtgtgtgaag cgtacccaca gacaccagac tgcaccaaga actagtatat ctcatagcct   1560 

tcacttgctc cccaaggccg tgactggcac actcccaatg gcaagagaca tgacatacca   1620 

tggttctgaa gtccctagtc atcgtcacgt ctgtgccacc tgtgccaagg ctctcgctcg   1680 

ggagacccag tctgcaggtt gactgtcctc catgcagata ggattggcaa gcccacctgt   1740 

ctgagggggc acttagccct caaggatgtc tgagggagca atggccccat gtactgtcca   1800 

ccctgacacc taccggctct gtcttccagc tccaatttgg ctcaagtgat gaccagatga   1860 

tgggtggacc aggagattgc tgcttgtggg gaccaactta ttctgttcct taccaggatg   1920 

ggaagagtct taacgtggtg gggggaggct ctggctgtgg aaggtgcctg ctgtgcgcca   1980 

tctctgaagc aggtttggtt gtagcagcgt tacctctgtc tagaggaaag cttgtgtggg   2040 

tctggtgcat ggttctctga ggtgggcatg gcagtgcctt tcctgtgtgt ctggggagga   2100 

aggctgcttt ctgtgaaatt ttctttctat ttttctattt ttagtactgt acggatgttc   2160 

tggaaccaca cacggtactc tgtgcttgct tgcatcttta ataaaagaca cctcccctgt   2220 

caaaaaaaaa aaaaaaaaa                                                2239 

 
           
             48  
             235  
             PRT  
             Rattus norvegicus  
             
               F1-ATPase alpha subunit  
             
           
            48 

Val Ala Asp Arg Gln Met Ser Leu Leu Leu Arg Arg Pro Pro Gly Arg 
1               5                   10                  15 

Glu Ala Tyr Pro Gly Asp Val Phe Tyr Leu His Ser Arg Leu Leu Glu 
            20                  25                  30 

Arg Ala Ala Lys Met Asn Asp Ser Phe Gly Gly Gly Ser Leu Thr Ala 
        35                  40                  45 

Leu Pro Val Ile Glu Thr Gln Ala Gly Asp Val Ser Ala Tyr Ile Pro 
    50                  55                  60 

Thr Asn Val Ile Ser Ile Thr Asp Gly Gln Ile Phe Leu Glu Thr Glu 
65                  70                  75                  80 

Leu Phe Tyr Lys Gly Ile Arg Pro Ala Ile Asn Val Gly Leu Ser Val 
                85                  90                  95 

Ser Arg Val Gly Ser Ala Ala Gln Thr Arg Ala Met Lys Gln Val Ala 
            100                 105                 110 

Gly Thr Met Lys Leu Glu Leu Ala Gln Tyr Arg Glu Val Ala Ala Phe 
        115                 120                 125 

Ala Gln Phe Gly Ser Asp Leu Asp Ala Ala Thr Gln Gln Leu Leu Ser 
    130                 135                 140 

Arg Gly Val Arg Leu Thr Glu Leu Leu Lys Gln Gly Gln Tyr Ser Pro 
145                 150                 155                 160 

Met Ala Ile Glu Glu Gln Val Ala Val Ile Tyr Ala Gly Val Arg Gly 
                165                 170                 175 

Tyr Leu Asp Lys Leu Glu Pro Ser Lys Ile Thr Lys Phe Glu Ser Ala 
            180                 185                 190 

Phe Leu Ser His Val Val Ser Gln His Gln Ser Leu Leu Gly Asn Ile 
        195                 200                 205 

Arg Ser Asp Gly Lys Ile Ser Glu Gln Ser Asp Ala Lys Leu Lys Glu 
    210                 215                 220 

Ile Val Thr Asn Phe Leu Ala Gly Phe Glu Pro 
225                 230                 235 

 
           
             49  
             1066  
             DNA  
             Rattus norvegicus  
             
               F1-ATPase alpha subunit  
             
           
            49 

gtggctgacc gccagatgtc tctgctgctc cgccgacccc cgggtcgaga ggcctatccc     60 

ggtgatgtgt tttacctaca ctctcgcctg ctggagagag cagccaagat gaacgattcc    120 

tttggtggtg gctctttgac tgccttacca gtcattgaaa cacaggctgg tgatgtgtcc    180 

gcctacattc caacaaacgt tatttccatc accgatggac agatcttctt ggaaacagaa    240 

ttgttctata aaggcatccg ccctgccatt aatgtgggct tgtctgtgtc ccgtgtcgga    300 

tctgccgccc agaccagagc catgaagcag gtggcaggca ccatgaagct ggagttggcc    360 

cagtaccggg aggtcgctgc ttttgcccag tttggttctg atctggatgc tgccactcag    420 

cagctcttga gccgtggcgt gcgcctgacc gagctgctaa agcaaggaca gtactctccc    480 

atggctattg aagaacaggt ggctgtcatc tatgcaggcg tccggggtta tcttgataaa    540 

ctggagccca gtaagatcac aaagttcgag agtgctttct tgtctcatgt tgtgagccag    600 

caccagagcc tcttgggcaa tatcaggtct gatgggaaaa tctcagaaca gtcggatgca    660 

aagctgaagg aaatcgtaac aaacttcttg gctgggtttg aaccttaaag ccctgccact    720 

gtcaccagac actgctttgg ttttgtcatt tattgtggta aaatcagcac catttgtaaa    780 

ggtttactct tgtactccct gatgtacaga aatcacatga ataaaagttc catattgagt    840 

gctagtttgt gtgggacggg ctcagatgta tcctgggact tgtggaggtc agagagcagc    900 

ctgtaggagt cagtgccctc ctttccctgg gaatttaact caggttatta tttaaagctc    960 

gacttcaggg gttggggatt tagctcagtg gtagagcgct tgcctaggaa gcgcaaggcc   1020 

ctgggttcgg tccccagctc cggaaaaaaa agaaccaacc cgaatt                  1066 

 
           
             50  
             168  
             PRT  
             Rattus norvegicus  
             
               F1F0 ATPase delta subunit  
             
           
            50 

Met Leu Pro Ala Ala Leu Leu Arg His Pro Gly Leu Arg Arg Leu Val 
1               5                   10                  15 

Leu Gln Ala Arg Thr Tyr Ala Gln Ala Ala Ala Ser Pro Ala Pro Ala 
            20                  25                  30 

Ala Gly Pro Gly Gln Met Ser Phe Thr Phe Ala Ser Pro Thr Gln Val 
        35                  40                  45 

Phe Phe Asp Gly Ala Asn Val Arg Gln Val Asp Val Pro Thr Leu Thr 
    50                  55                  60 

Gly Ala Phe Gly Ile Leu Ala Ser His Val Pro Thr Leu Gln Val Leu 
65                  70                  75                  80 

Arg Pro Gly Leu Val Met Val His Ala Glu Asp Gly Thr Thr Thr Lys 
                85                  90                  95 

Tyr Phe Val Ser Ser Gly Ser Val Thr Val Asn Ala Asp Ser Ser Val 
            100                 105                 110 

Gln Leu Leu Ala Glu Glu Val Val Thr Leu Asp Met Leu Asp Leu Gly 
        115                 120                 125 

Ala Ala Arg Ala Asn Leu Glu Lys Ala Gln Ser Glu Leu Ser Gly Ala 
    130                 135                 140 

Ala Asp Glu Ala Ala Arg Ala Glu Ile Gln Ile Arg Ile Glu Ala Asn 
145                 150                 155                 160 

Glu Ala Leu Val Lys Ala Leu Glu 
                165 

 
           
             51  
             811  
             DNA  
             Rattus norvegicus  
             
               F1F0 ATPase delta subunit  
             
           
            51 

ggaattccgc tgctgtcgct aagctaaagt cactgacgtt tccgccacca tgctgcccgc     60 

cgcattgctt cgtcacccag gtctgcgccg tctggtgctc caggcgcgta cgtacgccca    120 

ggccgccgcc tcacctgccc ccgccgctgg gcctggacag atgtccttca ccttcgcctc    180 

cccgacgcag gtgttctttg atggtgccaa tgtccggcaa gtggatgtgc ctacgctgac    240 

tggagccttt ggcatcctgg catcccatgt ccccacacta caggtcctac ggcctgggct    300 

ggtaatggtt catgcggaag atggcaccac aactaagtat tttgtgagca gcggctccgt    360 

cactgtgaat gcggactcct ctgtgcaatt actagctgaa gaagtcgtga cactggacat    420 

gctggacctc ggggcagccc gggcaaacct ggagaaggcg cagtcagagc tgtcaggggc    480 

agcagatgag gcagcacggg ctgagatcca aatccggatc gaggccaatg aagccctggt    540 

gaaggccctg gagtaggcgt actttgtctg tcacccacag ggtaacagag gcaggtcctg    600 

gggggctgaa gtcgccacca gggggcagca gtgctccggt tactggctta aagcttccct    660 

ggtgctgcct gccaggtcat ggagggcttc cccaggcttc cagagtctgg gatccccagg    720 

atgcctctgg agagctggcc ttgattgccc ctcaaagcca cccggacagt cagctggccc    780 

agcctatctg cattaaatac cacggaattc c                                   811 

 
           
             52  
             572  
             PRT  
             Homo sapiens  
             
               Dihydropyrimidinase-related protein  
             
           
            52 

Met Ser Tyr Gln Gly Lys Lys Asn Ile Pro Arg Ile Thr Ser Asp Arg 
1               5                   10                  15 

Leu Leu Ile Lys Gly Gly Lys Ile Val Asn Asp Asp Gln Ser Phe Tyr 
            20                  25                  30 

Ala Asp Ile Tyr Met Glu Asp Gly Leu Ile Lys Gln Ile Gly Glu Asn 
        35                  40                  45 

Leu Ile Val Pro Gly Gly Val Lys Thr Ile Glu Ala His Ser Arg Met 
    50                  55                  60 

Val Ile Pro Gly Gly Ile Asp Val His Thr Arg Phe Gln Met Pro Asp 
65                  70                  75                  80 

Gln Gly Met Thr Ser Ala Asp Asp Phe Phe Gln Gly Thr Lys Ala Ala 
                85                  90                  95 

Leu Ala Gly Gly Thr Thr Met Ile Ile Asp His Val Val Pro Glu Pro 
            100                 105                 110 

Gly Thr Ser Leu Leu Ala Ala Phe Asp Gln Trp Arg Glu Trp Ala Asp 
        115                 120                 125 

Ser Lys Ser Cys Cys Asp Tyr Ser Leu His Val Asp Ile Ser Glu Trp 
    130                 135                 140 

His Lys Gly Ile Gln Glu Glu Met Glu Ala Leu Val Lys Asp His Gly 
145                 150                 155                 160 

Val Asn Ser Phe Leu Val Tyr Met Ala Phe Lys Asp Arg Phe Gln Leu 
                165                 170                 175 

Thr Asp Cys Gln Ile Tyr Glu Val Leu Ser Val Ile Arg Asp Ile Gly 
            180                 185                 190 

Ala Ile Ala Gln Val His Ala Glu Asn Gly Asp Ile Ile Ala Glu Glu 
        195                 200                 205 

Gln Gln Arg Ile Leu Asp Leu Gly Ile Thr Gly Pro Glu Gly His Val 
    210                 215                 220 

Leu Ser Arg Pro Glu Glu Val Glu Ala Glu Ala Val Asn Arg Ala Ile 
225                 230                 235                 240 

Thr Ile Ala Asn Gln Thr Asn Cys Pro Leu Tyr Ile Thr Lys Val Met 
                245                 250                 255 

Ser Lys Ser Ser Ala Glu Val Ile Ala Gln Ala Arg Lys Lys Gly Thr 
            260                 265                 270 

Val Val Tyr Gly Glu Pro Ile Thr Ala Ser Leu Gly Thr Asp Gly Ser 
        275                 280                 285 

His Tyr Trp Ser Lys Asn Trp Ala Lys Ala Ala Ala Phe Val Thr Ser 
    290                 295                 300 

Pro Pro Leu Ser Pro Asp Pro Thr Thr Pro Asp Phe Leu Asn Ser Leu 
305                 310                 315                 320 

Leu Ser Cys Gly Asp Leu Gln Val Thr Gly Ser Ala His Cys Thr Phe 
                325                 330                 335 

Asn Thr Ala Gln Lys Ala Val Gly Lys Asp Asn Phe Thr Leu Ile Pro 
            340                 345                 350 

Glu Gly Thr Asn Gly Thr Glu Glu Arg Met Ser Val Ile Trp Asp Lys 
        355                 360                 365 

Ala Val Val Thr Gly Lys Met Asp Glu Asn Gln Phe Val Ala Val Thr 
    370                 375                 380 

Ser Thr Asn Ala Ala Lys Val Phe Asn Leu Tyr Pro Arg Lys Gly Arg 
385                 390                 395                 400 

Ile Ala Val Gly Ser Asp Ala Asp Leu Val Ile Trp Asp Pro Asp Ser 
                405                 410                 415 

Val Lys Thr Ile Ser Ala Lys Thr His Asn Ser Ser Leu Glu Tyr Asn 
            420                 425                 430 

Ile Phe Glu Gly Met Glu Cys Arg Gly Ser Pro Leu Val Val Ile Ser 
        435                 440                 445 

Gln Gly Lys Ile Val Leu Glu Asp Gly Thr Leu His Val Thr Glu Gly 
    450                 455                 460 

Ser Gly Arg Tyr Ile Pro Arg Lys Pro Phe Pro Asp Phe Val Tyr Lys 
465                 470                 475                 480 

Arg Ile Lys Ala Arg Ser Arg Leu Ala Glu Leu Arg Gly Val Pro Arg 
                485                 490                 495 

Gly Leu Tyr Asp Gly Pro Val Cys Glu Val Ser Val Thr Pro Lys Thr 
            500                 505                 510 

Val Thr Pro Ala Ser Ser Ala Lys Thr Ser Pro Ala Lys Gln Gln Ala 
        515                 520                 525 

Pro Pro Val Arg Asn Leu His Gln Ser Gly Phe Ser Leu Ser Gly Ala 
    530                 535                 540 

Gln Ile Asp Asp Asn Ile Pro Arg Arg Thr Thr Gln Arg Ile Val Ala 
545                 550                 555                 560 

Pro Pro Gly Gly Arg Ala Asn Ile Thr Ser Leu Gly 
                565                 570 

 
           
             53  
             4459  
             DNA  
             Homo sapiens  
             
               Dihydropyrimidinase-related protein  
             
           
            53 

gtttctctct ctccttctct ctctctctct ctctctcttt tttttccgcc ctagctgggg     60 

ctgtgttgga ggagaggaag aaagagagac agaggattgc attcatccgt tacgttcttg    120 

aaatttccta atagcaagac cagcgaagcg gttgcaccct tttcaatctt gcaaaggaaa    180 

aaaacaaaac aaaacaaaaa aaacccaagt ccccttcccg gcagtttttg ccttaaagct    240 

gccctcttga aattaatttt ttcccaggag agagatgtct tatcagggga agaaaaatat    300 

tccacgcatc acgagcgatc gtcttctgat caaaggaggt aaaattgtta atgatgacca    360 

gtcgttctat gcagacatat acatggaaga tgggttgatc aagcaaatag gagaaaatct    420 

gattgtgcca ggaggagtga agaccatcga ggcccactcc cggatggtga tccccggagg    480 

aattgacgtc cacactcgtt tccagatgcc tgatcaggga atgacgtctg ctgatgattt    540 

cttccaagga accaaggcgg ccctggctgg gggaaccact atgatcattg accacgttgt    600 

tcctgagcct gggacaagcc tgctcgctgc ctttgaccag tggagggaat gggccgacag    660 

caagtcctgc tgtgactact ctctgcatgt ggacatcagc gagtggcata agggcatcca    720 

ggaggagatg gaagcgcttg tgaaggatca cggggtaaat tccttcctcg tgtacatggc    780 

tttcaaagat cgcttccagc taacggattg ccagatttat gaagtactga gtgtgatccg    840 

ggatattggc gccatagccc aagtccacgc agaaaatggc gacatcattg cagaggagca    900 

gcagaggatc ctggatctgg gcatcacggg ccccgaggga catgtgctga gccgacctga    960 

ggaggtcgag gccgaagccg tgaatcgtgc catcaccatc gccaaccaga ccaactgccc   1020 

gctgtatatc accaaggtga tgagcaaaag ctctgctgag gtcatcgccc aggcacggaa   1080 

gaagggaact gtggtgtatg gcgagcccat cactgccagc ttgggaacgg acggctccca   1140 

ttactggagc aagaactggg ccaaggctgc tgcctttgtc acctccccac ccttgagccc   1200 

tgatccaacc actccagact ttctcaactc cttgctgtcc tgtggagacc tccaggtcac   1260 

gggcagtgcc cattgcacgt ttaacactgc ccagaaggct gtaggaaagg acaacttcac   1320 

cctgattccg gagggcacca atggcactga ggagcggatg tccgtcatct gggacaaggc   1380 

tgtggtcact gggaagatgg atgagaacca gtttgtggct gtgaccagca ccaatgcagc   1440 

caaagtcttc aacctttacc cccggaaagg ccgcattgct gtgggatccg atgccgacct   1500 

ggtcatctgg gaccccgaca gcgttaaaac catctctgcc aagacacaca acagctctct   1560 

cgagtacaac atctttgaag gcatggagtg ccgcggctcc ccactggtgg tcatcagcca   1620 

ggggaagatt gtcctggagg acggcaccct gcatgtcacc gaaggctctg gacgctacat   1680 

tccccggaag cccttccctg attttgttta caagcgtatc aaggcaagga gcaggctggc   1740 

tgagctgaga ggggttcctc gtggcctgta tgacggacct gtgtgtgaag tgtctgtgac   1800 

gcccaagaca gtcactccag cctcctcggc caagacgtct cctgccaagc agcaggcccc   1860 

acctgtccgg aacctgcacc agtctggatt cagtttgtct ggtgctcaga ttgatgacaa   1920 

cattccccgc cgcaccaccc agcgtatcgt ggcgcccccc ggtggccgtg ccaacatcac   1980 

cagcctgggc tagagctcct gggctgtgcg tccactgggg actggggatg ggacacctga   2040 

ggacattctg agacttcttt cttccttcct tttttttttt ttgttttttt ttttaagagc   2100 

ctgtgatagt tactgtggag cagccagttc atggggtccc ccttggggcc ccacaccccg   2160 

tctctcacca agagttactg attttgctca tccacttccc tacacatcta tgggtatcac   2220 

acccaagact acccaccaag ctcatacagg gaaccacacc caacacttag acatgcgaac   2280 

aagcagcccc cagcgagggt ctccttcgcc ttcaacctcc tagtgtctgt tagcatcttc   2340 

cttttcatgg ggggagggaa gataaagtga attgcccaga gctgcctttt tcttttcttt   2400 

ttaaaaattt taagaagttt tccttgtggg gctggggagg ggccggggtc agggagagtc   2460 

tttttttttt tttttttaaa tactaaattg gaacatttaa ttccatatta atacaagggg   2520 

tttgaactgg acatcctaat gatgcaatta cgtcatcacc cagctgattc cgggtggttg   2580 

gcaaactcat cgtgtctgtc ctgagaggct ccacaatgcc cacccgcatc gccattctgt   2640 

agtcttcagg gtcagctgtt gataaagggg caggcttgcg ttattggcct agattttgct   2700 

gcagattaaa tcctttgagg attctcttct cttttaccat ttttctgcgt gctctcactc   2760 

tctctttctc tctctagctt tttaattcat gaatattttc gtgtctgtct ctctctctct   2820 

ctgtgtttcc tccagccctt gtctcggaga cggtgttttc ctcccttgcc ccattatctt   2880 

ttcacctccc aggtctacca tttcatggtg gtcgttgggt ccgcctaaag gatttgagcg   2940 

tttgccattg caagcatagt gctgtgtcat cctggtccat gtaggactgg tgctaaccac   3000 

ctgccatcat gaggatgtgt gctagagtgt gggaccctgg ccaagtgcag gaatgggcca   3060 

tgccgtctca cccacagtat cacacgtgga accgcagaca gggcccagaa gctttagagg   3120 

tatgaggctg cagaaccgga gagattttcc tctgtgcagt gctctctggc taaagtcacg   3180 

gtcaaaccta aacaccgagc ctcattaacc caagtgaacc aaccaaagtc accagttcag   3240 

aagtgctaag ctaataggag tctgacccga gggcctgctg cttcctggtt aagtatcttt   3300 

tgagattcta gaacacatgg gagcttttta ttttcgggga aaaaccgtat ttttttcttg   3360 

tccaattatt tctaaagaca cactacatag aaagaggccc tataaactca aaaagtcatt   3420 

gggaaactta aagtctattc tactttgcaa gaggagaaat gtgttttatg aacgatagat   3480 

cacatcagaa ctcctgtggg gaggaaacct tataaattaa acacatggcc cccttagaga   3540 

ccacaggtga tgtctgtctc catccttccc tctccttttc tgtcaccttt ccccctagct   3600 

ggctcctttg gacctacccc tgtccttgct gacttgtgtt gcattgtatt ccaaacgtgt   3660 

ttacaggttc tcttaagcaa tgttgtattt gcaggctttt ctgaatacca aatctgcttt   3720 

ttgtaaagcg taaaaacatc acaaagtagg tcattccatc accacccttg tctctctaca   3780 

cattttgcct ttggggatct ggttggggtt ttgggttttt tgttgttgtt gtttatttgt   3840 

tattttaaag gtaaattgca cttttaaaaa aataattggt tgacttaata tatttgcttt   3900 

ttttctcacc tgcacttaga ggaaatttga acaagttgga aaaaaacaat ttttgtttca   3960 

attctaagaa acacttgcag ctctagtatt cacttgagtc ttcctgtttt tcctgtaccg   4020 

ggtcatggta atttttggtt gttttggttg ttttcttaaa aaacaagtta aaacctgacg   4080 

atttctgcag gctgtgtaag catgtttacc tgttggcttg ctttgtgtgt ctgttaaatg   4140 

aatgtcatat gtaaatgcta aaataaatcg acagtgtctc agaactgaat aactgcagtg   4200 

acttgatgct ctaaaacagt gtaggattta agaatagatg gtttttaatc ctggaaattg   4260 

tgattgtgac ccatgagtgg aggaactttc agttctaaag ctgataaagt gtgtagccag   4320 

aagagtactt ttttttttgt aaccactgtc ttgatggcaa aataattatg gtaaaaaaca   4380 

agtctcgtgt ttattattcc ttaagaactc tgtgttatat taccatggaa cgcctaataa   4440 

agcaaaatgt ggttgtttc                                                4459 

 
           
             54  
             1410  
             DNA  
             Rattus norvegicus  
             
               Myelin basic protein S  
             
           
            54 

ttcggcttcc gaaggcctga tgtgatggca tcacagaaga gaccctcaca gcgacacgga     60 

tccaagtact tggccacagc aagtaccatg gaccatgccc ggcatggctt cctcccaagg    120 

cacagagaca cgggcatcct tgactccatc gggcgcttct ttagcggtga caggggtgcg    180 

cccaagcggg gctctggcaa ggactcacac acaagaacta cccactacgg ctccctgccc    240 

cagaagtcgc agaggaccca agatgaaaac ccagtagtcc acttcttcaa gaacattgtg    300 

acacctcgta caccccctcc atcccaagga aaggggagag gcctgtccct cagcagattt    360 

agctggggag gaagagacag ccgctctgga tctcccatag caagacgctg agagcctccc    420 

tgctcagcct tcccgaatcc tgccctcggc ttcttaatat aactgcctta aacgtttaat    480 

tctacttgca ccaaatagct agttagagca gaccctctct taatcccgtg gggctgtgaa    540 

cgcggcgggc cagcccacgg caccctgact ggctaaaact gtttgtccct ttttatttga    600 

agattgagtt tcctcggggt cttctctgcc ccgacttgct ccccgtgtac cttggtcgac    660 

tccggaggtt caggtgcacg gacacccttt caagttcacc cctactccat cctcagactt    720 

tcttttcacg gcgaggcgca cccctccagc ttccgtgggc actgcggata gacaggcaca    780 

ccgccaagga gccagagagc atggcgcagg ggactatgtg gtccaggctt cctttgtttt    840 

ctttccccta aagagctttg tttttcctga caggatcaga cagtcttgga gtggcttaca    900 

caacgggggc ttgtggtatg tgagcacagg tcgggcagct gtgagagtcc agagtggggt    960 

ggccctgggg acgcttccag gccagcggtt ccctgcaccc caccagctga tttcgagcgt   1020 

ggcagaggga aggaaagggg cgagcgggct gggcaatgga cccgacagga aacggggact   1080 

taggggaaca cgctggagat gccatgtgtg gctgccgaag gtcaccatct ctcctcagtg   1140 

ctccccagag caggtgcttt taagaaccct gtttcctctc agagcctagg gagagtccaa   1200 

ggacatggcg catcaggaag tgggactgca ggagttctct ggtggcctcg tgctgtccct   1260 

ctggccactt ctcactttag ggtggtcacg gcagctcgcc atggcagtgc cattggtgca   1320 

cactaacctc atggaaaagt aaccattccc tgcctcttag aaagaactca ttcttagttt   1380 

taggagggtt cctgtcgctg aatcaagtcg                                    1410 

 
           
             55  
             698  
             PRT  
             Rattus norvegicus  
             
               Transferrin  
             
           
            55 

Met Arg Phe Ala Val Gly Ala Leu Leu Ala Cys Ala Ala Leu Gly Leu 
1               5                   10                  15 

Cys Leu Ala Val Pro Asp Lys Thr Val Lys Trp Cys Ala Val Ser Glu 
            20                  25                  30 

His Glu Asn Thr Lys Cys Ile Ser Phe Arg Asp His Met Lys Thr Val 
        35                  40                  45 

Leu Pro Ala Asp Gly Pro Arg Leu Ala Cys Val Lys Lys Thr Ser Tyr 
    50                  55                  60 

Gln Asp Cys Ile Lys Ala Ile Ser Gly Gly Glu Ala Asp Ala Ile Thr 
65                  70                  75                  80 

Leu Asp Gly Gly Trp Val Tyr Asp Ala Gly Leu Thr Pro Asn Asn Leu 
                85                  90                  95 

Lys Pro Val Ala Ala Glu Phe Tyr Gly Ser Leu Glu His Pro Gln Thr 
            100                 105                 110 

His Tyr Leu Ala Val Ala Val Val Lys Lys Gly Thr Asp Phe Gln Leu 
        115                 120                 125 

Asn Gln Leu Gln Gly Lys Lys Ser Cys His Thr Gly Leu Gly Arg Ser 
    130                 135                 140 

Ala Gly Trp Ile Ile Pro Ile Gly Leu Leu Phe Cys Asn Leu Pro Glu 
145                 150                 155                 160 

Pro Arg Lys Pro Leu Glu Lys Ala Val Ala Ser Phe Phe Ser Gly Ser 
                165                 170                 175 

Cys Val Pro Cys Ala Asp Pro Val Ala Phe Pro Gln Leu Cys Gln Leu 
            180                 185                 190 

Cys Pro Gly Cys Gly Cys Ser Pro Thr Gln Pro Phe Phe Gly Tyr Val 
        195                 200                 205 

Gly Ala Phe Lys Cys Leu Arg Asp Gly Gly Gly Asp Val Ala Phe Val 
    210                 215                 220 

Lys His Thr Thr Ile Phe Glu Val Leu Pro Gln Lys Ala Asp Arg Asp 
225                 230                 235                 240 

Gln Tyr Glu Leu Leu Cys Leu Asp Asn Thr Arg Lys Pro Val Asp Gln 
                245                 250                 255 

Tyr Glu Asp Cys Tyr Leu Ala Arg Ile Pro Ser His Ala Val Val Ala 
            260                 265                 270 

Arg Asn Gly Asp Gly Lys Glu Asp Leu Ile Trp Glu Ile Leu Lys Val 
        275                 280                 285 

Ala Gln Glu His Phe Gly Lys Gly Lys Ser Lys Asp Phe Gln Leu Phe 
    290                 295                 300 

Gly Ser Pro Leu Gly Lys Asp Leu Leu Phe Lys Asp Ser Ala Phe Gly 
305                 310                 315                 320 

Cys Tyr Gly Val Pro Pro Arg Met Asp Tyr Arg Leu Tyr Leu Gly His 
                325                 330                 335 

Ser Tyr Val Thr Ala Ile Arg Asn Gln Arg Glu Gly Val Cys Pro Glu 
            340                 345                 350 

Ala Ser Ile Asp Ser Ala Pro Val Lys Trp Cys Ala Leu Ser His Gln 
        355                 360                 365 

Glu Arg Ala Lys Cys Asp Glu Trp Ser Val Thr Ser Asn Gly Gln Ile 
    370                 375                 380 

Glu Cys Glu Ser Ala Glu Ser Thr Glu Asp Cys Ile Asp Lys Ile Val 
385                 390                 395                 400 

Asn Gly Glu Ala Asp Ala Met Ser Leu Asp Gly Gly His Ala Tyr Ile 
                405                 410                 415 

Ala Gly Gln Cys Gly Leu Val Pro Val Met Ala Glu Asn Tyr Asp Ile 
            420                 425                 430 

Ser Ser Cys Thr Asn Pro Gln Ser Asp Val Phe Pro Lys Gly Tyr Tyr 
        435                 440                 445 

Ala Val Ala Val Val Lys Ala Ser Asp Ser Ser Ile Asn Trp Asn Asn 
    450                 455                 460 

Leu Lys Gly Lys Lys Ser Cys His Thr Gly Val Asp Arg Thr Ala Gly 
465                 470                 475                 480 

Trp Asn Ile Pro Met Gly Leu Leu Phe Ser Arg Ile Asn His Cys Lys 
                485                 490                 495 

Phe Asp Glu Phe Phe Ser Gln Gly Cys Ala Pro Gly Tyr Lys Lys Asn 
            500                 505                 510 

Ser Thr Leu Cys Asp Leu Cys Ile Gly Pro Ala Lys Cys Ala Pro Asn 
        515                 520                 525 

Asn Arg Glu Gly Tyr Asn Gly Tyr Thr Gly Ala Phe Gln Cys Leu Val 
    530                 535                 540 

Glu Lys Gly Asp Val Ala Phe Val Lys His Gln Thr Val Leu Glu Asn 
545                 550                 555                 560 

Thr Asn Gly Lys Asn Thr Ala Ala Trp Ala Lys Asp Leu Lys Gln Glu 
                565                 570                 575 

Asp Phe Gln Leu Leu Cys Pro Asp Gly Thr Lys Lys Pro Val Thr Glu 
            580                 585                 590 

Phe Ala Thr Cys His Leu Ala Gln Ala Pro Asn His Val Val Val Ser 
        595                 600                 605 

Arg Lys Glu Lys Ala Ala Arg Val Ser Thr Val Leu Thr Ala Gln Lys 
    610                 615                 620 

Asp Leu Phe Trp Lys Gly Asp Lys Asp Cys Thr Gly Asn Phe Cys Leu 
625                 630                 635                 640 

Phe Arg Ser Ser Thr Lys Asp Leu Leu Phe Arg Asp Asp Thr Lys Cys 
                645                 650                 655 

Leu Thr Lys Leu Pro Glu Gly Thr Thr Tyr Glu Glu Tyr Leu Gly Ala 
            660                 665                 670 

Glu Tyr Leu Gln Ala Val Gly Asn Ile Arg Lys Cys Ser Thr Ser Arg 
        675                 680                 685 

Leu Leu Glu Ala Cys Thr Phe His Lys Ser 
    690                 695 

 
           
             56  
             2293  
             DNA  
             Rattus norvegicus  
             
               Transferrin  
             
           
            56 

cgtccgctcc tcgccacaca caccgagagg atgaggttcg ctgtgggtgc cctgctggct     60 

tgtgccgccc tgggactgtg tctggctgtc cctgacaaaa cggtcaaatg gtgcgcagtg    120 

tctgagcatg agaacaccaa gtgtatcagt ttccgtgacc acatgaaaac cgtccttcca    180 

gctgatggcc cccggcttgc ctgtgtgaag aaaacctcct atcaagattg catcaaggcc    240 

atttctggag gtgaagctga tgccattacc ttggatgggg gttgggtgta cgatgcaggc    300 

ctgactccca acaacctgaa gcctgtggca gcagagtttt atggatcact tgaacatcca    360 

cagacccact acttggctgt ggccgtggtg aagaagggaa cagacttcca gctgaaccag    420 

ctccagggca agaagtcctg ccacactggc ctgggcaggt ctgcaggctg gattatcccc    480 

attggcttac ttttctgtaa cttgccagag ccccgcaagc ctcttgagaa agctgtggcc    540 

agtttcttct cgggcagttg tgtcccctgt gcagatccag tggccttccc ccagctgtgt    600 

caactgtgtc caggctgtgg ctgctccccg actcaaccgt tctttggcta cgtaggcgcc    660 

ttcaagtgtc tgagagatgg aggtggagat gtggcctttg tcaagcatac aaccatattt    720 

gaggtcttgc cacagaaggc tgacagggat caatatgagc tgctctgcct tgacaatacc    780 

cgcaagccag tggatcagta tgaggactgc tacctagccc ggatcccttc tcatgctgtt    840 

gtggctcgaa atggagatgg caaagaggac ttgatctggg agatcctcaa agtggctcag    900 

gaacactttg gcaaaggcaa atcaaaagac ttccaactgt tcggctctcc tcttgggaaa    960 

gacctgctgt ttaaggattc tgcctttggc tgttacgggg tgcccccaag gatggactac   1020 

aggctgtacc tcggccacag ctatgtcact gccattcgaa atcagcggga aggcgtgtgc   1080 

ccggaggcct ccatcgacag cgcgccagtg aaatggtgtg cactgagtca ccaagagaga   1140 

gccaagtgtg atgagtggag cgtcacgagc aatgggcaga tagagtgtga gtcagcagag   1200 

agcactgagg actgcattga caagattgtg aatggagaag cagatgccat gagcttggat   1260 

ggaggtcatg cctacatagc aggccagtgt ggactagtgc ccgtcatggc agagaactat   1320 

gatatctctt cgtgtacaaa cccacaatca gatgtctttc ctaaagggta ttatgccgtg   1380 

gctgtggtga aggcatcaga ctccagcatc aactggaaca acctgaaagg caagaagtcc   1440 

tgccatactg gagtagacag aaccgccggc tggaacatcc ctatgggcct gctgttcagc   1500 

aggatcaacc actgcaagtt cgatgaattt ttcagtcaag gctgtgctcc tggctataag   1560 

aagaattcca ccctctgtga cctgtgtatt ggcccagcaa aatgtgctcc gaacaacaga   1620 

gagggatata atggttatac aggggctttc cagtgcctcg ttgagaaggg agacgtagcc   1680 

tttgtgaagc accagactgt cctggaaaac acgaacggaa agaacactgc tgcatgggct   1740 

aaggatctga agcaggaaga cttccagctg ctgtgccctg atggtaccaa gaagcctgta   1800 

accgagttcg ccacctgcca cctggcccaa gctccaaacc atgttgtggt ctcacgaaaa   1860 

gagaaggcag cccgggttag cactgtgctg actgcccaga aggatttatt ttggaaaggt   1920 

gacaaggact gcactggcaa tttctgtttg ttccggtctt ccaccaagga ccttctgttc   1980 

agagatgaca ccaagtgttt gactaaactt ccagaaggta ccacatatga agagtactta   2040 

ggagcagagt acttgcaagc tgttggaaac ataaggaagt gttcaacctc acgactccta   2100 

gaagcctgca ctttccacaa aagttaaaat ccaagaggtg ggtgccactg tggtggagga   2160 

ggatgccccc gtgatccatg ggcttctcct ggcctccata gccctgagcg gctggggcta   2220 

actgtgtccg tcttactgct tggtgttgtt accacataca cagagcacaa aataaaaaat   2280 

gactgttgac ttt                                                      2293 

 
           
             57  
             542  
             PRT  
             Rattus norvegicus  
             
               NF-L  
             
           
            57 

Met Ser Ser Phe Ser Tyr Glu Pro Tyr Phe Ser Thr Ser Tyr Lys Arg 
1               5                   10                  15 

Arg Tyr Val Glu Thr Pro Arg Val His Ile Ser Ser Val Arg Ser Gly 
            20                  25                  30 

Tyr Ser Thr Ala Arg Ser Ala Tyr Ser Ser Tyr Ser Ala Pro Val Ser 
        35                  40                  45 

Ser Ser Leu Ser Val Arg Arg Ser Tyr Ser Ser Ser Ser Gly Ser Leu 
    50                  55                  60 

Met Pro Ser Leu Glu Asn Leu Asp Leu Ser Gln Val Ala Ala Ile Ser 
65                  70                  75                  80 

Asn Asp Leu Lys Ser Ile Arg Thr Gln Glu Lys Ala Gln Leu Gln Asp 
                85                  90                  95 

Leu Asn Asp Arg Phe Ala Ser Phe Ile Glu Arg Val His Glu Leu Glu 
            100                 105                 110 

Gln Gln Asn Lys Val Leu Glu Ala Glu Leu Leu Val Leu Arg Gln Lys 
        115                 120                 125 

His Ser Glu Pro Ser Arg Phe Arg Ala Leu Tyr Glu Gln Glu Ile Arg 
    130                 135                 140 

Asp Leu Arg Leu Ala Ala Glu Asp Ala Thr Asn Glu Lys Gln Ala Leu 
145                 150                 155                 160 

Gln Gly Glu Arg Glu Gly Leu Glu Glu Thr Leu Arg Asn Leu Gln Ala 
                165                 170                 175 

Arg Tyr Glu Glu Glu Val Leu Ser Arg Glu Asp Ala Glu Gly Arg Leu 
            180                 185                 190 

Met Glu Ala Arg Lys Gly Ala Asp Glu Ala Ala Leu Ala Arg Ala Glu 
        195                 200                 205 

Leu Glu Lys Arg Ile Asp Ser Leu Met Asp Glu Ile Ala Phe Leu Lys 
    210                 215                 220 

Lys Val His Glu Glu Glu Ile Ala Glu Leu Gln Ala Gln Ile Gln Tyr 
225                 230                 235                 240 

Ala Gln Ile Ser Val Glu Met Asp Val Ser Ser Lys Pro Asp Leu Ser 
                245                 250                 255 

Ala Ala Leu Lys Asp Ile Arg Ala Gln Tyr Glu Lys Leu Ala Ala Lys 
            260                 265                 270 

Asn Met Gln Asn Ala Glu Glu Trp Phe Lys Ser Arg Phe Thr Val Leu 
        275                 280                 285 

Thr Glu Ser Ala Ala Lys Asn Thr Asp Ala Val Arg Ala Ala Lys Asp 
    290                 295                 300 

Glu Val Ser Glu Ser Arg Arg Leu Leu Lys Ala Lys Thr Leu Glu Ile 
305                 310                 315                 320 

Glu Ala Cys Arg Gly Met Asn Glu Ala Leu Glu Lys Gln Leu Gln Glu 
                325                 330                 335 

Leu Glu Asp Lys Gln Asn Ala Asp Ile Ser Ala Met Gln Asp Thr Ile 
            340                 345                 350 

Asn Lys Leu Glu Asn Glu Leu Arg Ser Thr Lys Ser Glu Met Ala Arg 
        355                 360                 365 

Tyr Leu Lys Glu Tyr Gln Asp Leu Leu Asn Val Lys Met Ala Leu Asp 
    370                 375                 380 

Ile Glu Ile Ala Ala Tyr Arg Lys Leu Leu Glu Gly Glu Glu Thr Arg 
385                 390                 395                 400 

Leu Ser Phe Thr Ser Val Gly Ser Ile Thr Ser Gly Tyr Ser Gln Ser 
                405                 410                 415 

Ser Gln Val Phe Gly Arg Ser Ala Tyr Ser Gly Leu Gln Ser Ser Ser 
            420                 425                 430 

Tyr Leu Met Ser Ala Arg Ala Phe Pro Ala Tyr Tyr Thr Ser His Val 
        435                 440                 445 

Gln Glu Glu Gln Ser Glu Val Glu Glu Thr Ile Glu Ala Thr Lys Ala 
    450                 455                 460 

Glu Glu Ala Lys Asp Glu Pro Pro Ser Glu Gly Glu Ala Glu Glu Glu 
465                 470                 475                 480 

Glu Lys Glu Lys Glu Glu Gly Glu Glu Glu Glu Gly Ala Glu Glu Glu 
                485                 490                 495 

Glu Ala Ala Lys Asp Glu Ser Glu Asp Ala Lys Glu Glu Glu Gly Gly 
            500                 505                 510 

Glu Gly Glu Glu Glu Asp Thr Lys Glu Ser Glu Glu Glu Glu Lys Lys 
        515                 520                 525 

Glu Glu Ser Ala Gly Glu Glu Gln Ala Ala Lys Lys Lys Asp 
    530                 535                 540 

 
           
             58  
             2032  
             DNA  
             Rattus norvegicus  
             
               NF-L  
             
           
            58 

gaattccccc ggttcttctc tctaggtccc ctatctccgc cccgggcctg aggggcgcac     60 

cgaccgccac catgagttcg ttcagctacg agccgtactt ttcgacctcc tacaagcggc    120 

gctacgtgga gacgccccgg gtgcacatct ccagcgtgcg cagcggctac agcacggcgc    180 

gctctgcgta ctccagctac tccgcgcccg tctcctcctc tctgtcggtg cgccgcagct    240 

actcatccag ctccggctct ttgatgccca gcctggagaa cctcgatctg agccaggtag    300 

ccgccatcag caacgacctc aagtctatcc gcacacagga gaaggcacag ctgcaggacc    360 

tcaacgatcg cttcgccagc ttcatcgagc gcgtgcacga gctggagcag cagaacaagg    420 

tcctggaagc cgagctgttg gtgctgcgcc agaagcactc agagccttcc cgcttccgcg    480 

ccctgtatga gcaggagatc cgtgatctgc gactggcggc cgaagacgcc actaacgaga    540 

agcaggcgct gcagggcgag cgcgaggggc tggaggagac tctgcgcaac ctgcaggctc    600 

gctacgagga ggaggtgctg agccgcgagg acgccgaggg ccggctgatg gaagcccgca    660 

aaggcgcgga tgaggctgcg ctcgcccgcg ccgagctgga gaagcgcatc gacagcctga    720 

tggacgagat agccttcctg aaaaaggtgc acgaggaaga gatcgccgag ctgcaggctc    780 

agatccagta tgctcagatc tccgtggaga tggacgtgtc ctccaagccc gacctctccg    840 

ccgctctcaa ggacatccgc gctcagtacg agaagctggc cgccaagaat atgcagaatg    900 

ccgaagagtg gttcaagagc cgcttcacgg tgctaaccga gagcgccgcc aagaacaccg    960 

acgcagtgcg cgctgccaag gacgaggtgt cggaaagccg ccgcctactt aaggctaaga   1020 

ccctggagat cgaagcctgc cggggtatga acgaagctct agagaagcag ctgcaggagc   1080 

tggaggacaa gcagaatgca gacatcagcg ccatgcagga cacaatcaac aaactggaga   1140 

atgagctgcg aagcacgaag agcgagatgg ccaggtacct gaaggagtac caggacctcc   1200 

tcaatgtcaa gatggcattg gacattgaga ttgcagctta caggaaactc ttggaaggcg   1260 

aagaaaccag gctcagtttc accagcgtgg gtagcataac cagcggctac tctcagagct   1320 

cgcaggtctt tggccgttct gcttacagtg gcttgcagag cagctcctac ttgatgtctg   1380 

ctcgagcatt cccagcctac tataccagcc acgtccagga agagcagtca gaggtggagg   1440 

agaccattga ggctacgaaa gctgaggagg ccaaggatga gcccccctct gaaggagaag   1500 

cagaagagga ggagaaggag aaagaggagg gggaggaaga ggaaggtgct gaggaggaag   1560 

aagctgccaa ggatgagtct gaagatgcca aagaagaaga aggtggtgag ggtgaagagg   1620 

aagacaccaa agagtcagaa gaggaagaga agaaagagga gagtgctggg gaggagcaag   1680 

ctgctaagaa gaaagattga gccccattcc caactatccc aggaaaaaag tctccccaaa   1740 

tcaggtcaac ctcatcacca aaccaaccag ttgagttcca gatcctatac agattaagaa   1800 

gtcaatatat gtataattct gagatgactt aggttggaca ttcaatgttg tgctatgact   1860 

ttcctcctta tgcagagtat ctgtttgctt gcagagtggc tttctggctt gctgccaacc   1920 

tgtgcatggt ccatgcttat gagttcagga tctacggcaa tgtgaatcac acagatgttt   1980 

acaataataa taataaaaaa accacacata cacaacatga ataaatgaat tc           2032 

 
           
             59  
             626  
             PRT  
             Rattus norvegicus  
             
               MAG  
             
           
            59 

Met Ile Phe Leu Thr Thr Leu Pro Leu Phe Trp Ile Met Ile Ser Ala 
1               5                   10                  15 

Ser Arg Gly Gly His Trp Gly Ala Trp Met Pro Ser Ser Ile Ser Ala 
            20                  25                  30 

Phe Glu Gly Thr Cys Val Ser Ile Pro Cys Arg Phe Asp Phe Pro Asp 
        35                  40                  45 

Glu Leu Arg Pro Ala Val Val His Gly Val Trp Tyr Phe Asn Ser Pro 
    50                  55                  60 

Tyr Pro Lys Asn Tyr Pro Pro Val Val Phe Lys Ser Arg Thr Gln Val 
65                  70                  75                  80 

Val His Glu Ser Phe Gln Gly Arg Ser Arg Leu Leu Gly Asp Leu Gly 
                85                  90                  95 

Leu Arg Asn Cys Thr Leu Leu Leu Ser Thr Leu Ser Pro Glu Leu Gly 
            100                 105                 110 

Gly Lys Tyr Tyr Phe Arg Gly Asp Leu Gly Gly Tyr Asn Gln Tyr Thr 
        115                 120                 125 

Phe Ser Glu His Ser Val Leu Asp Ile Ile Asn Thr Pro Asn Ile Val 
    130                 135                 140 

Val Pro Pro Glu Val Val Ala Gly Thr Glu Val Glu Val Ser Cys Met 
145                 150                 155                 160 

Val Pro Asp Asn Cys Pro Glu Leu Arg Pro Glu Leu Ser Trp Leu Gly 
                165                 170                 175 

His Glu Gly Leu Gly Glu Pro Thr Val Leu Gly Arg Leu Arg Glu Asp 
            180                 185                 190 

Glu Gly Thr Trp Val Gln Val Ser Leu Leu His Phe Val Pro Thr Arg 
        195                 200                 205 

Glu Ala Asn Gly His Arg Leu Gly Cys Gln Ala Ala Phe Pro Asn Thr 
    210                 215                 220 

Thr Leu Gln Phe Glu Gly Tyr Ala Ser Leu Asp Val Lys Tyr Pro Pro 
225                 230                 235                 240 

Val Ile Val Glu Met Asn Ser Ser Val Glu Ala Ile Glu Gly Ser His 
                245                 250                 255 

Val Ser Leu Leu Cys Gly Ala Asp Ser Asn Pro Pro Pro Leu Leu Thr 
            260                 265                 270 

Trp Met Arg Asp Gly Met Val Leu Arg Glu Ala Val Ala Glu Ser Leu 
        275                 280                 285 

Tyr Leu Asp Leu Glu Glu Val Thr Pro Ala Glu Asp Gly Ile Tyr Ala 
    290                 295                 300 

Cys Leu Ala Glu Asn Ala Tyr Gly Gln Asp Asn Arg Thr Val Glu Leu 
305                 310                 315                 320 

Ser Val Met Tyr Ala Pro Trp Lys Pro Thr Val Asn Gly Thr Val Val 
                325                 330                 335 

Ala Val Glu Gly Glu Thr Val Ser Ile Leu Cys Ser Thr Gln Ser Asn 
            340                 345                 350 

Pro Asp Pro Ile Leu Thr Ile Phe Lys Glu Lys Gln Ile Leu Ala Thr 
        355                 360                 365 

Val Ile Tyr Glu Ser Gln Leu Gln Leu Glu Leu Pro Ala Val Thr Pro 
    370                 375                 380 

Glu Asp Asp Gly Glu Tyr Trp Cys Val Ala Glu Asn Gln Tyr Gly Gln 
385                 390                 395                 400 

Arg Ala Thr Ala Phe Asn Leu Ser Val Glu Phe Ala Pro Ile Ile Leu 
                405                 410                 415 

Leu Glu Ser His Cys Ala Ala Ala Arg Asp Thr Val Gln Cys Leu Cys 
            420                 425                 430 

Val Val Lys Ser Asn Pro Glu Pro Ser Val Ala Phe Glu Leu Pro Ser 
        435                 440                 445 

Arg Asn Val Thr Val Asn Glu Thr Glu Arg Glu Phe Val Tyr Ser Glu 
    450                 455                 460 

Arg Ser Gly Leu Leu Leu Thr Ser Ile Leu Thr Leu Arg Gly Gln Ala 
465                 470                 475                 480 

Gln Ala Pro Pro Arg Val Ile Cys Thr Ser Arg Asn Leu Tyr Gly Thr 
                485                 490                 495 

Gln Ser Leu Glu Leu Pro Phe Gln Gly Ala His Arg Leu Met Trp Ala 
            500                 505                 510 

Lys Ile Gly Pro Val Gly Ala Val Val Ala Phe Ala Ile Leu Ile Ala 
        515                 520                 525 

Ile Val Cys Tyr Ile Thr Gln Thr Arg Arg Lys Lys Asn Val Thr Glu 
    530                 535                 540 

Ser Pro Ser Phe Ser Ala Gly Asp Asn Pro His Val Leu Tyr Ser Pro 
545                 550                 555                 560 

Glu Phe Arg Ile Ser Gly Ala Pro Asp Lys Tyr Glu Ser Glu Lys Arg 
                565                 570                 575 

Leu Gly Ser Glu Arg Arg Leu Leu Gly Leu Arg Gly Glu Pro Pro Glu 
            580                 585                 590 

Leu Asp Leu Ser Tyr Ser His Ser Asp Leu Gly Lys Arg Pro Thr Lys 
        595                 600                 605 

Asp Ser Tyr Thr Leu Thr Glu Glu Leu Ala Glu Tyr Ala Glu Ile Arg 
    610                 615                 620 

Val Lys 
625 

 
           
             60  
             2475  
             DNA  
             Rattus norvegicus  
             
               MAG  
             
           
            60 

cagaagccag accatccaac cttctgtatc agtgctcctc gtcgcctcac tgtacttcac     60 

ggaagagact tggttgactg gccacttgga gcggaatcag gagacattcc caactcaggg    120 

agactgaggt gagggcccta gctcgcccac ttgctggaca agatgatatt ccttaccacc    180 

ctgcctctgt tttggataat gatttcagct tctcgagggg ggcactgggg tgcctggatg    240 

ccctcgtcca tctcagcctt cgagggcacg tgtgtctcca tcccctgccg tttcgacttc    300 

ccggatgagc tcagaccggc tgtggtacat ggcgtctggt atttcaacag tccctacccc    360 

aagaactacc cgccagtggt cttcaagtcc cgcacacaag tggtccacga gagcttccag    420 

ggccgtagcc gcctgttggg agacctgggc ctacgaaact gcaccctgct tctcagcacg    480 

ctgagccctg agctgggagg gaaatactat ttccgaggtg acctgggcgg ctacaaccag    540 

tacaccttct cggagcacag cgtcctggac atcatcaaca cccccaacat cgtggtgccc    600 

ccagaagtgg tggcaggaac ggaagtagag gtcagctgca tggtgccgga caactgccca    660 

gagctgcgcc ctgagctgag ctggctgggc cacgaggggc taggggagcc cactgttctg    720 

ggtcggctgc gggaggatga aggcacctgg gtgcaggtgt cactgctaca cttcgtgcct    780 

actagagagg ccaacggcca ccgtctgggc tgtcaggctg ccttccccaa caccaccttg    840 

cagttcgagg gttacgccag tctggacgtc aagtaccccc cggtgattgt ggagatgaat    900 

tcctctgtgg aggccattga gggctcccat gtcagcctgc tctgtggggc tgacagcaac    960 

ccgccaccgc tgctgacttg gatgcgggat gggatggtgt tgagggaggc agttgctgag   1020 

agcctgtacc tggatctgga ggaggtgacc ccagcagagg acggcatcta tgcttgcctg   1080 

gcagagaatg cctatggcca ggacaaccgc acggtggagc tgagcgtcat gtatgcacct   1140 

tggaagccca cagtgaatgg gacggtggtg gcggtagagg gggagacagt ctccatcctg   1200 

tgttccacac agagcaaccc ggaccctatt ctcaccatct tcaaggagaa gcagatcctg   1260 

gccacggtca tctatgagag tcagctgcag ctggaactcc ctgcagtgac gcccgaggac   1320 

gatggggagt actggtgtgt agctgagaac cagtatggcc agagagccac cgccttcaac   1380 

ctgtctgtgg agtttgctcc cataatcctt ctggaatcgc actgtgcagc ggccagagac   1440 

accgtgcagt gcctgtgtgt ggtaaaatcc aacccggaac cctccgtggc ctttgagctg   1500 

ccttcccgca acgtgactgt gaacgagaca gagagggagt ttgtgtactc agagcgcagc   1560 

ggcctcctgc tcaccagcat cctcacgctc cggggtcagg cccaagcccc accccgcgtc   1620 

atttgtacct ccaggaacct ctacggcacc cagagcctcg agctgccttt ccagggagca   1680 

caccgactga tgtgggccaa aatcggccct gtgggtgctg tggtcgcctt tgccatcctg   1740 

attgccattg tctgctacat cacccagaca agaagaaaaa agaacgtcac agagagcccc   1800 

agcttctcag cgggagacaa ccctcatgtc ctgtacagcc ccgaattccg aatctctgga   1860 

gcacctgata agtatgagag tgagaagcgc ctggggtccg agaggaggct gctgggcctt   1920 

aggggggaac ccccagaact ggacctcagt tattcccact cagacctggg gaaacgaccc   1980 

accaaggaca gctacaccct gacagaggag ctggctgagt acgcagaaat ccgagtcaag   2040 

tgaggaagct gggggctggc cctgtggctc accccccatc aggaccctcg cttggccccc   2100 

actggccgtg ggctcccttt ctcttgagag tggtaggggt gggggcggga aggggcgggg   2160 

caggaaacag tgaggtctta ggggcccggc ctcccctcct tcccggctgc tcctctctgc   2220 

caacatcctg cacctatgtt acagctccct ctcccctcct tttaacctca gctgttgaga   2280 

ggggtgctct gtctgtccat gttatttatt gttatcctgg tctcctgtcc ccttacccgg   2340 

ccccaggacc tgtacaaaag ggacatgaaa taaatgtcct aatgacaagt gccagtctag   2400 

acccatcctt tggaggaaag gggcatatta gtaatacttt tctcgttgct gtaacaaaat   2460 

actggacaaa aacac                                                    2475 

 
           
             61  
             845  
             PRT  
             Rattus norvegicus  
             
               NF-M protein  
             
           
            61 

Met Ser Tyr Thr Leu Asp Ser Leu Gly Asn Pro Ser Ala Tyr Arg Arg 
1               5                   10                  15 

Val Pro Thr Glu Thr Arg Ser Ser Phe Ser Arg Val Ser Gly Ser Pro 
            20                  25                  30 

Ser Ser Gly Phe Arg Ser Gln Ser Trp Ser Arg Gly Ser Pro Ser Thr 
        35                  40                  45 

Val Ser Ser Ser Tyr Lys Arg Ser Ala Leu Ala Pro Arg Leu Ala Tyr 
    50                  55                  60 

Ser Ser Ala Met Leu Ser Ser Ala Glu Ser Ser Leu Asp Phe Ser Gln 
65                  70                  75                  80 

Ser Ser Ser Leu Leu Asn Gly Gly Ser Gly Gly Asp Tyr Lys Leu Ser 
                85                  90                  95 

Arg Ser Asn Glu Lys Glu Gln Leu Gln Gly Leu Asn Asp Arg Phe Ala 
            100                 105                 110 

Gly Tyr Ile Glu Lys Val His Tyr Leu Glu Gln Gln Asn Lys Glu Ile 
        115                 120                 125 

Glu Ala Glu Ile His Ala Leu Arg Gln Lys Gln Ala Ser His Ala Gln 
    130                 135                 140 

Leu Gly Asp Ala Tyr Asp Gln Glu Ile Arg Glu Leu Arg Ala Thr Leu 
145                 150                 155                 160 

Glu Met Val Asn His Glu Lys Ala Gln Val Gln Leu Asp Ser Asp His 
                165                 170                 175 

Leu Glu Glu Asp Ile His Arg Leu Lys Glu Arg Phe Glu Glu Glu Ala 
            180                 185                 190 

Arg Leu Arg Asp Asp Thr Glu Ala Ala Ile Arg Ala Val Arg Lys Asp 
        195                 200                 205 

Ile Glu Glu Ser Ser Met Val Lys Val Glu Leu Asp Lys Lys Val Gln 
    210                 215                 220 

Ser Leu Gln Asp Glu Val Ala Phe Leu Arg Ser Asn His Glu Glu Glu 
225                 230                 235                 240 

Val Ala Asp Leu Leu Ala Gln Ile Gln Ala Ser His Ile Thr Val Glu 
                245                 250                 255 

Arg Lys Asp Tyr Leu Lys Thr Asp Ile Ser Thr Ala Leu Lys Glu Ile 
            260                 265                 270 

Arg Ser Gln Leu Glu Cys His Ser Asp Gln Asn Met His Gln Ala Glu 
        275                 280                 285 

Glu Trp Phe Lys Cys Arg Tyr Ala Lys Leu Thr Glu Ala Ala Glu Gln 
    290                 295                 300 

Asn Lys Glu Ala Ile Arg Ser Ala Lys Glu Glu Ile Ala Glu Tyr Arg 
305                 310                 315                 320 

Arg Gln Leu Gln Ser Lys Ser Ile Glu Leu Glu Ser Val Arg Gly Thr 
                325                 330                 335 

Lys Glu Ser Leu Glu Arg Gln Leu Ser Asp Ile Glu Glu Arg His Asn 
            340                 345                 350 

His Asp Leu Ser Ser Tyr Gln Asp Thr Ile Gln Gln Leu Glu Asn Glu 
        355                 360                 365 

Leu Arg Gly Thr Lys Trp Glu Met Ala Arg His Leu Arg Glu Tyr Gln 
    370                 375                 380 

Asp Leu Leu Asn Val Lys Met Ala Leu Asp Ile Glu Ile Ala Ala Tyr 
385                 390                 395                 400 

Arg Lys Leu Leu Glu Gly Glu Glu Thr Arg Phe Ser Thr Phe Ser Gly 
                405                 410                 415 

Ser Ile Thr Gly Pro Leu Tyr Thr His Arg Gln Pro Ser Val Thr Ile 
            420                 425                 430 

Ser Ser Lys Ile Gln Lys Thr Lys Val Glu Ala Pro Lys Leu Lys Val 
        435                 440                 445 

Gln His Lys Phe Val Glu Glu Ile Ile Glu Glu Thr Lys Val Glu Asp 
    450                 455                 460 

Glu Lys Ser Glu Met Glu Asp Ala Leu Thr Val Ile Ala Glu Glu Leu 
465                 470                 475                 480 

Ala Ala Ser Ala Lys Glu Glu Lys Glu Glu Ala Glu Glu Lys Glu Glu 
                485                 490                 495 

Glu Pro Glu Val Lys Ser Pro Val Lys Ser Pro Glu Ala Lys Glu Glu 
            500                 505                 510 

Glu Glu Gly Glu Lys Glu Glu Glu Glu Glu Gly Gln Glu Glu Glu Glu 
        515                 520                 525 

Glu Glu Asp Glu Gly Val Lys Ser Asp Gln Ala Glu Glu Gly Gly Ser 
    530                 535                 540 

Glu Lys Glu Gly Ser Ser Glu Lys Asp Glu Gly Glu Gln Glu Glu Glu 
545                 550                 555                 560 

Gly Glu Thr Glu Ala Glu Gly Glu Gly Glu Glu Ala Glu Ala Lys Glu 
                565                 570                 575 

Glu Lys Lys Thr Glu Gly Lys Val Glu Glu Met Ala Ile Lys Glu Glu 
            580                 585                 590 

Ile Lys Val Glu Lys Pro Glu Lys Ala Lys Ser Pro Val Pro Lys Ser 
        595                 600                 605 

Pro Val Glu Glu Val Lys Pro Lys Pro Glu Ala Lys Ala Gly Lys Asp 
    610                 615                 620 

Glu Gln Lys Glu Glu Glu Lys Val Glu Glu Lys Lys Glu Val Ala Lys 
625                 630                 635                 640 

Glu Ser Pro Lys Glu Glu Lys Val Glu Lys Lys Glu Glu Lys Pro Lys 
                645                 650                 655 

Asp Val Pro Asp Lys Lys Lys Ala Glu Ser Pro Val Lys Glu Lys Ala 
            660                 665                 670 

Val Glu Glu Met Ile Thr Ile Thr Lys Ser Val Lys Val Ser Leu Glu 
        675                 680                 685 

Lys Asp Thr Lys Glu Glu Lys Pro Gln Gln Gln Glu Lys Val Lys Glu 
    690                 695                 700 

Lys Ala Glu Glu Glu Gly Gly Ser Glu Glu Glu Val Gly Asp Lys Ser 
705                 710                 715                 720 

Pro Gln Glu Ser Lys Lys Glu Asp Ile Ala Ile Asn Gly Glu Val Glu 
                725                 730                 735 

Gly Lys Glu Glu Glu Glu Gln Glu Thr Gln Glu Lys Gly Ser Gly Gln 
            740                 745                 750 

Glu Glu Glu Lys Gly Val Val Thr Asn Gly Leu Asp Val Ser Pro Ala 
        755                 760                 765 

Glu Glu Lys Lys Gly Glu Asp Arg Ser Asp Asp Lys Val Val Val Thr 
    770                 775                 780 

Lys Lys Val Glu Lys Ile Thr Ser Glu Gly Gly Asp Gly Ala Thr Lys 
785                 790                 795                 800 

Tyr Ile Thr Lys Ser Val Thr Val Thr Gln Lys Val Glu Glu His Glu 
                805                 810                 815 

Glu Thr Phe Glu Glu Lys Leu Val Ser Thr Lys Lys Val Glu Lys Val 
            820                 825                 830 

Thr Ser His Ala Ile Val Lys Glu Val Thr Gln Gly Asp 
        835                 840                 845 

 
           
             62  
             2717  
             DNA  
             Rattus norvegicus  
             
               NF-M protein  
             
           
            62 

ggcctccaag atgagctaca cgctggactc gctgggcaac ccgtccgcct accggcgcgt     60 

tccaaccgag acccggtcca gcttcagtcg tgtgagcggt tccccgtcca gcggcttccg    120 

ctcgcagtcc tggtcccgcg gctcgcccag caccgtgtcc tcctcctaca agcgcagcgc    180 

gctcgccccg cgcctcgcct acagctcggc tatgctcagc tcggccgaga gcagcctcga    240 

cttcagccag tcctcttcgc tgcttaacgg cggctccggc ggcgactaca agctgtcccg    300 

ctcaaacgag aaagagcagc tgcaggggct gaacgaccgt ttcgccggct acatcgagaa    360 

agtgcactac ttggaacaac agaacaagga gatcgaggca gagatccacg cgctgcggca    420 

gaagcaggcc tcgcacgccc agctgggtga cgcttacgac caggagatcc gagagctgcg    480 

cgccaccctg gagatggtga atcacgagaa ggctcaagtg cagctggact ctgatcactt    540 

ggaggaagac atccaccggc tcaaggagcg cttcgaggag gaggcgcggc tgcgggacga    600 

cacggaggct gccatccggg cggtgcgcaa agacatagag gagtcgtcga tggttaaggt    660 

ggagctggac aagaaggttc agtcgctgca ggatgaggtg gccttcctgc ggagcaatca    720 

cgaagaggag gtggccgacc tgctggccca gatccaggcg tcgcacatca ccgtagagcg    780 

caaagactac ctgaagacag acatctccac ggcgctgaaa gagatccgct cccagctcga    840 

gtgtcactcc gaccagaaca tgcaccaggc cgaagagtgg ttcaaatgcc gctacgccaa    900 

gctcaccgag gcggccgagc agaacaagga ggccatccgc tccgctaaag aagagatcgc    960 

cgagtaccgg cgccagctgc agtccaagag cattgagctc gagtcggtgc gaggcactaa   1020 

ggagtccctg gaacggcagc tcagcgacat cgaggagcgc cacaaccacg acctcagcag   1080 

ctaccaggac accatccagc agctggaaaa tgagcttcgg ggaacaaagt gggaaatggc   1140 

tcgtcatttg cgagaatacc aggatctcct taacgtcaag atggctctgg acatcgagat   1200 

cgccgcatat aggaaactac tggagggtga agagaccaga tttagcacat tttcaggaag   1260 

catcactggg cctctgtaca cacaccgaca gccctcagtc acaatatcca gtaagattca   1320 

gaagaccaaa gtcgaggccc ccaagctcaa ggtccaacac aaatttgtgg aggagatcat   1380 

tgaggagact aaagtggaag atgagaagtc agaaatggaa gacgccctca cagtcattgc   1440 

agaggaattg gcagcctctg ccaaagagga gaaagaagag gcagaagaaa aggaagagga   1500 

accggaagtg aagtctcccg tgaagtctcc tgaggctaag gaagaggagg aaggggaaaa   1560 

ggaggaagaa gaggaaggcc aagaggaaga agaggaggaa gatgaaggtg tcaagtcaga   1620 

ccaggcagaa gagggaggat ctgagaagga aggctcgagt gaaaaggatg aaggtgagca   1680 

agaagaagaa ggggaaactg aggcagaagg tgaaggagag gaagcagaag ctaaggagga   1740 

aaagaaaaca gagggaaagg tcgaggaaat ggctatcaag gaggaaatca aggtcgagaa   1800 

gcccgagaaa gccaagtccc ctgtgccaaa atcaccggtg gaagaagtaa agccaaaacc   1860 

agaagccaaa gccggaaagg atgagcagaa ggaggaagag aaagttgagg agaagaagga   1920 

ggtagccaag gaatcaccca aggaagagaa ggtggagaaa aaggaggaga agccaaaaga   1980 

tgtcccagat aaaaagaagg ctgagtcccc agtgaaagaa aaggccgtag aggaaatgat   2040 

caccattact aagtcggtaa aggtgagcct ggagaaagac accaaagagg agaagcctca   2100 

gcagcaggag aaggtgaagg agaaggcaga ggaggagggg ggtagtgagg aggaagtggg   2160 

tgacaaaagc ccgcaagaat ccaagaagga agacatagct atcaatgggg aggtggaagg   2220 

aaaagaggag gaggagcagg aaactcagga gaagggcagt gggcaagagg aggagaaagg   2280 

ggtggtcact aatggcttag atgtgagccc tgcggaggaa aagaaagggg aggatagaag   2340 

tgatgacaaa gtggtggtga ccaagaaggt agaaaaaatc accagcgagg gaggcgatgg   2400 

tgctaccaaa tacatcacca aatctgttac tgtcactcaa aaggttgaag agcatgagga   2460 

gacctttgag gagaagctgg tgtcaactaa aaaggtagaa aaggtcactt cacatgccat   2520 

agtcaaggaa gtcacccagg gtgactaaga tcggagtcca ttgcaaaagg ttaagccata   2580 

caacaatttc aaaatgcatg tgattgacag cttcaaaaca gaatgggttc tcccatgagg   2640 

gctccagaca ttgtattttc ctttgtgcaa tatgagggaa ctgcatgcaa gctcagggtg   2700 

cctcctcagt ccttggg                                                  2717 

 
           
             63  
             707  
             PRT  
             Rattus norvegicus  
             
               Neurodegeneration associated protein 1  
             
           
            63 

Met Ser Gln Tyr Thr Glu Lys Glu Pro Ser Val Met Asp Gln Asp Ser 
1               5                   10                  15 

Ser Lys Ala Ala Trp Pro Arg Ala Ala Gly Gly Tyr Gln Thr Ile Thr 
            20                  25                  30 

Gly Arg Arg Tyr Gly Arg Arg His Ala Tyr Val Ser Phe Lys Pro Cys 
        35                  40                  45 

Met Thr Arg His Glu Arg Ser Leu Gly Arg Ala Gly Asp Asp Tyr Glu 
    50                  55                  60 

Val Leu Glu Leu Asp Asp Val Ala Lys Glu Asn Thr Ala Gly Ser Ser 
65                  70                  75                  80 

Ser Leu Asp Gln Val His Pro Ser Leu Pro Ser Glu Thr Thr Val Glu 
                85                  90                  95 

Lys Ser Glu Thr Glu Ile Pro Thr Cys Gly Pro Ala Leu Asn Gln Ser 
            100                 105                 110 

Thr Glu Ser Asn Pro Ser Val Ala Thr Val Cys His Ser Glu Glu Val 
        115                 120                 125 

Arg Glu Thr Leu Asp Ser Ser Thr Asn Leu Gln Asn His Ala Glu Arg 
    130                 135                 140 

Glu Cys Thr Pro Ala Val Cys Asn Ala Ser Ser Val Gln Asn Gly Ile 
145                 150                 155                 160 

Val Leu Val His Thr Asp Ser Tyr Asp Pro Asp Ser Lys His Asp Glu 
                165                 170                 175 

Asn Asp Ser Leu Gln Leu Cys Ala Gln Ala Val Glu Gly Gly Arg Arg 
            180                 185                 190 

Gln Lys Val Leu Gly Asn Ala Val Phe Glu Leu Glu Asn Gly Glu Val 
        195                 200                 205 

Glu Arg Tyr Ala Asp Leu Cys Pro Ser Val Pro Ser Leu Ser Gly Glu 
    210                 215                 220 

Ile Arg Glu Glu Ser Glu Glu Leu Gly Ser Ala Leu Leu Glu Lys Asn 
225                 230                 235                 240 

Ser Ala Gly Asp Ala Glu Ala Val His Gln Asp Gly Gln Glu Phe Gln 
                245                 250                 255 

Arg Ser Ser Glu Asp Gly Ile Val Arg Lys Arg Arg Gln Asp Asp Thr 
            260                 265                 270 

Asp Gln Gly Arg Gln Thr Glu Asn Ser Thr Glu Asp Ala Asp Cys Val 
        275                 280                 285 

Pro Gly His Val Glu Gln Asn Thr Ser Glu Arg Ala Asn His His Gly 
    290                 295                 300 

Ser Ser Pro Glu Gln Val Val Arg Pro Lys Val Arg Lys Val Ile Ser 
305                 310                 315                 320 

Ser Ser Gln Val Asp Gln Glu Ser Gly Phe Asn Arg His Glu Ala Lys 
                325                 330                 335 

Gln Arg Ser Val Gln Arg Trp Arg Glu Ala Leu Glu Val Glu Glu Cys 
            340                 345                 350 

Ser Ser Asp Asp Pro Ile Ile Lys Cys Asp Asp Tyr Asp Gly Asp His 
        355                 360                 365 

Asp Cys Met Phe Leu Thr Pro Ser Tyr Ser Arg Val Thr Pro Arg Glu 
    370                 375                 380 

Ala Glu Arg His Arg Ala Thr Ala Glu Asn Gly Ala Thr Ala Ser Gly 
385                 390                 395                 400 

Arg Gln Glu Ala Arg Glu Asn Ala Phe Trp Asn Ala Cys Gly Glu Tyr 
                405                 410                 415 

Tyr Gln Leu Phe Asp Lys Asp Glu Asp Ser Ser Glu Cys Ser Asp Gly 
            420                 425                 430 

Glu Trp Ser Ala Ser Leu Pro His Arg Phe Ser Gly Thr Glu Lys Asp 
        435                 440                 445 

Gln Ser Ser Ser Asp Glu Ser Trp Glu Thr Leu Pro Gly Lys Asp Glu 
    450                 455                 460 

Asn Glu Pro Glu Leu Gln Ser Asp Ser Ser Gly Pro Glu Glu Glu Asn 
465                 470                 475                 480 

Gln Glu Leu Ser Leu Gln Glu Gly Glu Gln Thr Ser Leu Glu Glu Gly 
                485                 490                 495 

Glu Ile Pro Trp Leu Gln Tyr Asn Glu Val Asn Glu Ser Ser Ser Asp 
            500                 505                 510 

Glu Gly Asn Glu Pro Ala Asn Glu Phe Ala Gln Pro Glu Ala Phe Met 
        515                 520                 525 

Leu Asp Gly Asn Asn Asn Leu Glu Asp Asp Ser Ser Val Ser Glu Asp 
    530                 535                 540 

Leu Asp Val Asp Trp Ser Leu Phe Asp Gly Phe Ala Asp Gly Leu Gly 
545                 550                 555                 560 

Val Ala Glu Ala Ile Ser Tyr Val Asp Pro Gln Phe Leu Thr Tyr Met 
                565                 570                 575 

Ala Leu Glu Glu Arg Leu Ala Gln Ala Met Glu Thr Ala Leu Ala His 
            580                 585                 590 

Leu Glu Ser Leu Ala Val Asp Val Glu Val Ala Asn Pro Pro Ala Ser 
        595                 600                 605 

Lys Glu Ser Ile Asp Gly Leu Pro Glu Thr Leu Val Leu Glu Asp His 
    610                 615                 620 

Thr Ala Ile Gly Gln Glu Gln Cys Cys Pro Ile Cys Cys Ser Glu Tyr 
625                 630                 635                 640 

Ile Lys Asp Asp Ile Ala Thr Glu Leu Pro Cys His His Phe Phe His 
                645                 650                 655 

Lys Pro Cys Val Ser Ile Trp Leu Gln Lys Ser Gly Thr Cys Pro Val 
            660                 665                 670 

Cys Arg Arg His Phe Pro Pro Ala Val Ile Asp Ala Ser Ala Ala Ala 
        675                 680                 685 

Ser Ser Glu Pro Asp Leu Asp Ala Ser Pro Ala Asn Asp Asn Ala Glu 
    690                 695                 700 

Glu Ala Pro 
705 

 
           
             64  
             4758  
             DNA  
             Rattus norvegicus  
             
               Neurodegeneration associated protein 1  
             
           
            64 

tggcgggtgg aggatcgagc gctgttctcg ctctggagcc gctgcacatc tcggaatcat     60 

ttgcagctta ttcactgtgt aaggaaaaac caaattacat ccataattct attgaactgg    120 

aagcatagac gctgccatat atgtcacagt atacagaaaa agaaccatca gtaatggatc    180 

aagactccag caaggctgcg tggcctagag cagcaggagg ataccagacg attacaggca    240 

ggaggtacgg aaggagacat gcgtatgtca gttttaagcc atgtatgacc aggcatgaac    300 

ggagcttggg tcgggctggc gatgactatg aagttctgga actggatgac gttgccaagg    360 

aaaacaccgc aggttccagt tcattggatc aagtccatcc ttctttaccc agtgaaacta    420 

cagttgaaaa aagtgaaaca gaaattccta cttgtggtcc agcactgaat caaagcacgg    480 

agagcaaccc atccgttgcc acagtgtgtc atagtgagga agtcagggag accttagaca    540 

gcagtacgaa tcttcagaat cacgctgaga gagagtgtac gccagcagtt tgtaatgcct    600 

caagtgtcca gaatggaatt gtgttggttc atactgactc ttatgatcca gacagcaagc    660 

atgatgagaa tgactctctt caactttgtg cccaagctgt ggaaggtggt agacgtcaga    720 

aggtattagg caatgcagtc tttgagctgg aaaatggaga ggtagagaga tatgctgatc    780 

tgtgtccctc agttccctct ctcagtggtg aaataaggga ggagtctgaa gagctaggtt    840 

cagcactctt agagaaaaat tctgctggcg atgcggaggc tgtccatcag gatgggcagg    900 

aatttcagag gtcttctgaa gacggcattg ttagaaagag gcgacaagat gataccgatc    960 

agggaagaca gacagaaaat tcaactgaag atgcagactg tgttccaggc catgttgaac   1020 

aaaatactag tgagagagcc aatcaccatg gaagttctcc tgaacaggta gtgaggccca   1080 

aagttagaaa agtgatcagt tcaagccagg tggaccaaga gagcggtttt aataggcacg   1140 

aggctaagca aagaagtgtt cagaggtgga gagaggctct ggaagttgag gaatgcagtt   1200 

cagatgaccc tataatcaag tgtgacgatt atgatggaga ccatgactgc atgttcctaa   1260 

ccccatccta ctcaagagtt acgccaaggg aagcagaacg tcaccgtgcg acagcagaaa   1320 

atggagccac agcttcagga aggcaagagg ctcgggaaaa tgccttttgg aatgcctgtg   1380 

gagagtatta ccagctcttt gacaaagacg aagacagttc agagtgcagt gatggggaat   1440 

ggtctgcttc tctgcctcac cgattttctg gcacagaaaa agaccagtcc tcaagcgatg   1500 

aaagctggga aactctgcca ggaaaagatg agaatgaacc tgagctacag agtgatagta   1560 

gtggccctga ggaagaaaac caagaattgt ctcttcagga gggagaacag acgtccttgg   1620 

aggaggggga gattccctgg ttacagtaca atgaggtcaa tgagagcagc agcgatgaag   1680 

ggaacgagcc tgccaatgag tttgcacaac cagaagcttt catgttggat gggaacaaca   1740 

acctggagga cgactcgagc gtgagtgaag acctggatgt ggactggagc ctatttgatg   1800 

gttttgcgga tggacttggt gttgctgaag ctatttccta cgtggatcct cagttcctca   1860 

cctacatggc actagaagaa cgcttagccc aggctatgga gactgcactg gcacacttag   1920 

agtctcttgc tgtagacgtt gaggtggcta acccacctgc cagtaaggaa agcatcgacg   1980 

gacttccaga aacccttgtc ctagaagatc acaccgctat tggtcaggag cagtgctgtc   2040 

ccatctgctg cagtgagtac attaaggatg acatcgcaac agagctgccc tgccatcact   2100 

tctttcataa gccttgtgtc tccatttggc tacagaagtc cggaacgtgc cctgtgtgcc   2160 

gccgccactt cccacctgca gtgattgacg catctgcagc tgcttcctct gaaccagacc   2220 

ttgatgcctc gcctgcaaac gacaatgctg aggaagcccc gtaagccgtc acggggaaat   2280 

gagatcgaaa ttctatcaca gtaaatctgc aaattccttc taaatctgat gtgcaaataa   2340 

ttatatataa atatatttaa aatgctctat atagtatatg ccgtagttta gaaagagaat   2400 

attaaccttt ctaaactgaa tttaggtttg cagaagatac taaacatttt caagctgaat   2460 

gttgaagcag tgcatccatt ttctttagtt gaacatggtg tccattgtaa cgtcagtagt   2520 

catctctgtg gcatacgttt ctgttttgtc tgaagtgctg ccactaagag atcagaatta   2580 

agctctcctt tccacatcag atgtgaaagg agagcttaat tctgatctct gtggcatacg   2640 

tttctgtttt gtctgaagtg ctgccactaa gagatcagaa ttaagctctc ctttccacat   2700 

cagatgtgaa aactgtgatc acaacagtag tacagtttgg tttcattgaa aataaactga   2760 

attctaaagc atgctttttc actggtccct ttgcttttgc tacttcgaga cctcttggtt   2820 

tatataacac tgaggttaag attaaagctt ttcagaatgc caggcaaaga ctagagtttt   2880 

gacccgaaca cacacacaaa ataaaaaaat aaaaaataaa ttacatctta gatgtaaact   2940 

tcctgaaact gtcatgtaat aaactattca gtgaacgttg gatgtttaaa ggctagtaaa   3000 

gcatgtgaat tatctgatgt gcttcatgct cggcacagtc tcatgagctt ggcctatgaa   3060 

acactgtcta agccctcatg cagtctgctt ctacttgtta aacactgaag gtccagacca   3120 

gtcttggcat ctgattaaga aattggatgt agttagctaa ggtagtttgg gttctacttt   3180 

aggagatctg aagatttgca catggccatg tctgttacag catcacccca gattagaaag   3240 

gcacagcttt taggtggtca cacgagtagg caagtctgtg cagacgctgg tgtgataggt   3300 

agcttacagg tgagccctga catcagcaca gggacaacgg ctcagcaggc ccagcactgg   3360 

agcccatccg cgtgcacatc tacctgccct gctcatccag ttcgggaata tttctatagc   3420 

ttcccaaatc agcttcatct gatttgtgta atatatctga gtatttatag tctttctttt   3480 

ttctccagtc ttaaaaataa atgaattttc actgttggca catttgaggc ttaaatataa   3540 

gaaacctaac acttgcattc tgatttttgc atatattgta aatgtggctg gtatttacag   3600 

cgaatcctgt gtgtcctttt atgggtaaaa caaaagtgaa cattgcatgc aagtaatgtg   3660 

gtgaatttgt aatttagggg ttttctgggg cttctgtgac tgtggatgct taaattcagg   3720 

ccttaatgtt gctctagcta gcatgtttcc ttctaatgta catagtcagc ctgtatccac   3780 

taatctctgc ttgtcttctc ctccgtggtc ttcacaccgt atccactgat ggtcagttag   3840 

tgtcagaggt cacccaggtt agcatcagtt cccatgtgtg ccgtgcggat tgtgagccgt   3900 

gtggttgctt ggttggtttt cttttatgta ctttgattag ctgtggcact tactgagtaa   3960 

acttacgttg ctgcagactg ctgtgtaaca agccacgttt tgtgtttagt cagtattaca   4020 

cacgggacct tgttttattc ttctagatag cagctgtccc aaagaaaata tttcttcttt   4080 

gcctattaag atttagctat tatctgccag ttgttaaggg gtttgattcc aaactcaacc   4140 

agagttgaac gtaagaaagc tgctgtactc ttgctgtcca tctgttacgt cctccgttgt   4200 

tggctcctga ctacgtaccc acggctgctg aaggagactt gttttaattt gttttaaaaa   4260 

ggaaaatgaa gttttaggac tttgatggtg gggaagctga caagttcaga ccccagtctg   4320 

tcatttgctc ttagcttgga tccttttgaa aagttaagaa tatatgaagg taaattagaa   4380 

aaagtaaaaa tattgataaa atgtcattta tcttatgtct attttatgtt gttatttggc   4440 

ctattaattt taattttttg aacattttct tatttcttgt aatatgaatg ccgatattta   4500 

aagtttgact cacttgggtt tcttttgtgt ttcttagttg ttatctctaa gcctaactaa   4560 

cttttaagat tatttgtgat atggatttat ataagctgtt aaatatatat gagctgttaa   4620 

aatggaatgc aaggttttcc aaagaccagg tctaactgta atgattggtt tattgttcta   4680 

caatcccagc ccggcatttt ccgtgtaaat cataaacaat aaacaggata tactcagtgt   4740 

tcatttctaa aaaaaaaa                                                 4758 

 
           
             65  
             1488  
             DNA  
             Rattus norvegicus  
             
               Brain S-100 protein beta-subunit  
             
           
            65 

aagtccacac ccagtcctct ctggaggaag aaaagggagc ttctctgtct accctcctag     60 

tccttggaca ccgaagccag agaggactcc ggcggcaaaa ggtgaccagg agcctccggg    120 

atgtctgagc tggagaaggc catggttgcc ctcattgatg tcttccatca gtattcaggg    180 

agagagggtg acaagcacaa gctgaagaag tcagaactga aggagctcat caacaacgag    240 

ctctctcact tcctggagga aatcaaagag caggaagtgg tggacaaagt gatggagacg    300 

ctggacgaag atggggatgg ggagtgtgac ttccaggagt ttatggcctt cgtctccatg    360 

gtgaccacag cctgtcatga gttctttgaa catgagtgag acaaaaaaaa aaaaaaaaaa    420 

gtggctgagc cgtttccccg tggcagacat gagggccacg agaggaggca cggcagaagg    480 

ctcgtgggct ggaaggagct gcgctctcta gacgcatata actaattagg aagcttgatt    540 

tgcttcaggg atgaaactct gaccccgttc ccaagggctg ctttaagtta gcacttcgtt    600 

tctgctacac taggtattcc tgtgagctga atggtcccgg gaactattga taagagtcac    660 

tgagggacga aatcaacact ctgtgggtat agcactggtt gtagaccacc atgctcctgt    720 

ggaagggtca cctgtaagaa tcaaggcaga ctaccaatag cacctccgtt ggacagcttt    780 

cttaggtgta atgtatgctg tccatgcatc tacagaccca cagctggatc cactgccacc    840 

cgaagaggtt ggctcgccct tacaactgct tgtcctctgt gcaaacgatg ccccggaaag    900 

ttagacctat cacccacacc ctcccaccac ccccaggcca aaaggacagc ccacccaagt    960 

cccctccccc acagcgaatc gcggtttgtt accaagtacg tatttgacat caacagttcc   1020 

aactggtgga acgattagat ctcgcacact aagtattagc accctaactc acgaccgaga   1080 

atcaaaattc tgctcagtag acgtctcctt tcaggatgac accattgtcc ccataggaca   1140 

cggacagagg agggcactgg agagagtgtc aggtcttttt ctagctgtat cttcctctct   1200 

ccctctgctg cccataatgt gagtgaccct ctagggtgag acttgcaggg tgagctgctg   1260 

aggaatgaag ggccactgag atgtgtcctt tagctgctgg gtgtcatgtc tgacctgctg   1320 

gtgcctaggg cctgcttaac actcggcaag gctgcgagcc gaggactgtg ggaagccgga   1380 

cttgatgctt tctaacctgc atatttgaat gccgaaggtc aaacaatcca agttacagat   1440 

aaataaaaac cgcattgcaa gtattaaaaa gccattctag gaaaattc                1488 

 
           
             66  
             2364  
             PRT  
             Rattus norvegicus  
             
               Microtubule associated protein IB  
             
           
            66 

Gly Gln Lys Ile Leu His His Arg Ser Asp Val Leu Glu Thr Val Val 
1               5                   10                  15 

Leu Ile Asn Pro Ser Asp Glu Ala Val Ser Thr Glu Val Arg Leu Met 
            20                  25                  30 

Ile Thr Asp Ala Ala Arg His Lys Leu Leu Val Leu Thr Gly Gln Cys 
        35                  40                  45 

Phe Glu Asn Thr Gly Glu Leu Ile Leu Gln Ser Gly Ser Phe Ser Phe 
    50                  55                  60 

Gln Asn Phe Ile Glu Ile Phe Thr Asp Gln Glu Ile Gly Glu Leu Leu 
65                  70                  75                  80 

Ser Thr Thr His Pro Ala Asn Lys Ala Ser Leu Thr Leu Phe Cys Pro 
                85                  90                  95 

Glu Glu Gly Asp Trp Lys Asn Ser Asn Leu Asp Arg His Asn Leu Gln 
            100                 105                 110 

Asp Phe Ile Asn Ile Lys Leu Asn Ser Ala Ser Ile Leu Pro Glu Met 
        115                 120                 125 

Glu Gly Leu Ser Glu Phe Thr Glu Tyr Leu Ser Glu Ser Val Glu Val 
    130                 135                 140 

Pro Ser Pro Phe Asp Ile Leu Glu Pro Pro Thr Ser Gly Gly Phe Leu 
145                 150                 155                 160 

Lys Leu Ser Lys Pro Cys Cys Tyr Ile Phe Pro Gly Gly Arg Gly Asp 
                165                 170                 175 

Ser Ala Leu Phe Ala Val Asn Gly Phe Asn Met Leu Ile Asn Gly Gly 
            180                 185                 190 

Ser Glu Arg Lys Ser Cys Phe Trp Lys Leu Ile Arg His Leu Asp Arg 
        195                 200                 205 

Val Asp Ser Ile Leu Leu Thr His Ile Gly Asp Asp Asn Leu Pro Gly 
    210                 215                 220 

Ile Asn Ser Met Leu Gln Arg Lys Ile Ala Glu Leu Glu Glu Glu Ser 
225                 230                 235                 240 

Gln Gly Ser Thr Ser Asn Ser Asp Trp Met Lys Asn Leu Ile Ser Pro 
                245                 250                 255 

Asp Leu Gly Val Val Phe Leu Asn Val Pro Glu Asn Leu Lys Asn Pro 
            260                 265                 270 

Glu Pro Asn Ile Lys Met Lys Arg Ser Thr Glu Glu Ala Cys Phe Thr 
        275                 280                 285 

Leu Gln Tyr Leu Asn Lys Leu Ser Met Lys Pro Glu Pro Leu Phe Arg 
    290                 295                 300 

Ser Val Gly Asn Ala Ile Glu Pro Val Ile Leu Phe Gln Lys Met Gly 
305                 310                 315                 320 

Val Gly Lys Leu Lys Met Tyr Val Leu Asn Pro Val Lys Ser Ser Lys 
                325                 330                 335 

Glu Met Gln Tyr Phe Met Gln Gln Trp Thr Gly Thr Asn Lys Asp Lys 
            340                 345                 350 

Ala Glu Leu Ile Leu Pro Asn Gly Gln Glu Val Asp Ile Pro Ile Ser 
        355                 360                 365 

Tyr Leu Ala Ser Val Ser Ser Leu Ile Val Trp His Pro Ala Asn Pro 
    370                 375                 380 

Ala Glu Lys Ile Ile Arg Val Leu Phe Pro Gly Asn Ser Thr Gln Tyr 
385                 390                 395                 400 

Asn Ile Leu Glu Gly Leu Glu Lys Leu Lys His Leu Asp Phe Leu Lys 
                405                 410                 415 

Gln Pro Leu Ala Thr Gln Lys Asp Leu Thr Gly Gln Val Ser Thr Pro 
            420                 425                 430 

Pro Val Lys Gln Val Lys Leu Lys Gln Arg Ala Asp Ser Arg Glu Ser 
        435                 440                 445 

Leu Lys Pro Ala Thr Lys Pro Leu Ser Ser Lys Ser Val Arg Lys Glu 
    450                 455                 460 

Ser Lys Glu Glu Ala Pro Glu Ala Thr Lys Ala Ser Gln Val Glu Lys 
465                 470                 475                 480 

Thr Pro Lys Val Glu Ser Lys Glu Lys Val Ile Val Lys Lys Asp Lys 
                485                 490                 495 

Pro Gly Lys Val Glu Ser Lys Pro Ser Val Thr Glu Lys Glu Val Pro 
            500                 505                 510 

Ser Lys Glu Glu Gln Ser Pro Val Lys Ala Glu Val Ala Glu Lys Ala 
        515                 520                 525 

Ala Thr Glu Ser Lys Pro Lys Val Thr Lys Asp Lys Val Val Lys Lys 
    530                 535                 540 

Glu Ile Lys Thr Lys Pro Glu Glu Lys Lys Glu Glu Lys Pro Lys Lys 
545                 550                 555                 560 

Glu Val Ala Lys Lys Glu Asp Lys Thr Pro Leu Lys Lys Asp Glu Lys 
                565                 570                 575 

Pro Lys Lys Glu Glu Ala Lys Lys Glu Ile Lys Lys Glu Ile Lys Lys 
            580                 585                 590 

Glu Glu Lys Lys Glu Leu Lys Lys Glu Val Lys Lys Glu Thr Pro Leu 
        595                 600                 605 

Lys Asp Ala Lys Lys Glu Val Lys Lys Asp Glu Lys Lys Glu Val Lys 
    610                 615                 620 

Lys Glu Glu Lys Glu Pro Lys Lys Glu Ile Lys Lys Ile Ser Lys Asp 
625                 630                 635                 640 

Ile Lys Lys Ser Thr Pro Leu Ser Asp Thr Lys Lys Pro Ala Ala Leu 
                645                 650                 655 

Lys Pro Lys Val Ala Lys Lys Glu Glu Pro Thr Lys Lys Glu Pro Ile 
            660                 665                 670 

Ala Ala Gly Lys Leu Lys Asp Lys Gly Lys Val Lys Val Ile Lys Lys 
        675                 680                 685 

Glu Gly Lys Thr Thr Glu Ala Ala Ala Thr Ala Val Gly Thr Ala Ala 
    690                 695                 700 

Val Ala Ala Ala Ala Gly Val Ala Ala Ser Gly Pro Ala Lys Glu Leu 
705                 710                 715                 720 

Glu Ala Glu Arg Ser Leu Met Ser Ser Pro Glu Asp Leu Thr Lys Asp 
                725                 730                 735 

Phe Glu Glu Leu Lys Ala Glu Glu Ile Asp Val Ala Lys Asp Ile Lys 
            740                 745                 750 

Pro Gln Leu Glu Leu Ile Glu Asp Glu Glu Lys Leu Lys Glu Thr Glu 
        755                 760                 765 

Pro Gly Glu Ala Tyr Val Ile Gln Lys Glu Thr Glu Val Ser Lys Gly 
    770                 775                 780 

Ser Ala Glu Ser Pro Asp Glu Gly Ile Thr Thr Thr Glu Gly Glu Gly 
785                 790                 795                 800 

Glu Cys Glu Gln Thr Pro Glu Glu Leu Glu Pro Val Glu Lys Gln Gly 
                805                 810                 815 

Val Asp Asp Ile Glu Lys Phe Glu Asp Glu Gly Ala Gly Phe Glu Glu 
            820                 825                 830 

Ser Ser Glu Ala Gly Asp Tyr Glu Glu Lys Ala Glu Thr Glu Glu Ala 
        835                 840                 845 

Glu Glu Pro Glu Glu Asp Gly Glu Asp Asn Val Ser Gly Ser Ala Ser 
    850                 855                 860 

Lys His Ser Pro Thr Glu Asp Glu Glu Ile Ala Lys Ala Glu Ala Asp 
865                 870                 875                 880 

Val His Ile Lys Glu Lys Arg Glu Ser Val Ala Ser Gly Asp Asp Arg 
                885                 890                 895 

Ala Glu Glu Asp Met Asp Glu Ala Leu Glu Lys Gly Glu Ala Glu Gln 
            900                 905                 910 

Ser Glu Glu Glu Gly Glu Glu Glu Glu Asp Lys Ala Glu Asp Ala Arg 
        915                 920                 925 

Glu Glu Asp His Glu Pro Asp Lys Thr Glu Ala Glu Asp Tyr Val Met 
    930                 935                 940 

Ala Val Val Asp Lys Ala Ala Glu Ala Gly Val Thr Glu Asp Gln Tyr 
945                 950                 955                 960 

Asp Phe Leu Gly Thr Pro Ala Lys Gln Pro Gly Val Gln Ser Pro Ser 
                965                 970                 975 

Arg Glu Pro Ala Ser Ser Ile His Asp Glu Thr Leu Pro Gly Gly Ser 
            980                 985                 990 

Glu Ser Glu Ala Thr Ala Ser Asp Glu Glu Asn Arg Glu Asp Gln Pro 
        995                 1000                1005 

Glu Glu Phe Thr Ala Thr Ser Gly Tyr Thr Gln Ser Thr Ile Glu Ile 
    1010                1015                1020 

Ser Ser Glu Pro Thr Pro Met Asp Glu Met Ser Thr Pro Arg Asp Val 
 1025               1030                1035                1040 

Met Thr Asp Glu Thr Asn Asn Glu Glu Thr Glu Ser Pro Ser Gln Glu 
                1045                1050                1055 

Phe Val Asn Ile Thr Lys Tyr Glu Ser Ser Leu Tyr Ser Gln Glu Tyr 
            1060                1065                1070 

Ser Lys Pro Val Val Ala Ser Phe Asn Gly Leu Ser Asp Gly Ser Lys 
        1075                1080                1085 

Thr Asp Ala Thr Asp Gly Arg Asp Tyr Asn Ala Ser Ala Ser Thr Ile 
    1090                1095                1100 

Ser Pro Pro Ser Ser Met Glu Glu Asp Lys Phe Ser Lys Ser Ala Leu 
1105                1110                1115                1120 

Arg Asp Ala Tyr Arg Pro Glu Glu Thr Asp Val Lys Thr Gly Ala Glu 
                1125                1130                1135 

Leu Asp Ile Lys Asp Val Ser Asp Glu Arg Leu Ser Pro Ala Lys Ser 
            1140                1145                1150 

Pro Ser Leu Ser Pro Ser Pro Pro Ser Pro Ile Glu Lys Thr Pro Leu 
        1155                1160                1165 

Gly Glu Arg Ser Val Asn Phe Ser Leu Thr Pro Asn Glu Ile Lys Ala 
    1170                1175                1180 

Ser Ala Glu Gly Glu Ala Thr Ala Val Val Ser Pro Gly Val Thr Gln 
1185                1190                1195                1200 

Ala Val Val Glu Glu His Cys Ala Ser Pro Glu Glu Lys Thr Leu Glu 
                1205                1210                1215 

Val Val Ser Pro Ser Gln Ser Val Thr Gly Ser Ala Gly His Thr Pro 
            1220                1225                1230 

Tyr Tyr Gln Ser Pro Thr Asp Glu Lys Ser Ser His Leu Pro Thr Glu 
        1235                1240                1245 

Val Thr Glu Asn Ala Gln Ala Val Pro Val Ser Phe Glu Phe Thr Glu 
    1250                1255                1260 

Ala Lys Asp Glu Asn Glu Arg Ser Ser Ile Ser Pro Met Asp Glu Pro 
1265                1270                1275                1280 

Val Pro Asp Ser Glu Ser Pro Ile Glu Lys Val Leu Ser Pro Leu Arg 
                1285                1290                1295 

Ser Pro Pro Leu Ile Gly Ser Glu Ser Ala Tyr Glu Asp Phe Leu Ser 
            1300                1305                1310 

Ala Asp Asp Lys Ala Leu Gly Arg Arg Ser Glu Ser Pro Phe Glu Gly 
        1315                1320                1325 

Lys Asn Gly Lys Gln Gly Phe Ser Asp Lys Glu Ser Pro Val Ser Asp 
    1330                1335                1340 

Leu Thr Ser Asp Leu Tyr Gln Asp Lys Gln Glu Glu Lys Arg Ala Gly 
1345                1350                1355                1360 

Phe Ile Pro Ile Lys Glu Asp Phe Ser Pro Glu Lys Lys Ala Ser Asp 
                1365                1370                1375 

Ala Glu Ile Met Ser Ser Gln Ser Ala Leu Ala Leu Asp Glu Arg Lys 
            1380                1385                1390 

Leu Gly Gly Asp Gly Ser Pro Thr Gln Val Asp Val Ser Gln Phe Gly 
        1395                1400                1405 

Ser Phe Lys Glu Asp Thr Lys Met Ser Ile Ser Glu Gly Thr Val Ser 
    1410                1415                1420 

Asp Lys Ser Ala Thr Pro Val Asp Glu Gly Ala Glu Asp Thr Tyr Ser 
1425                1430                1435                1440 

His Met Glu Gly Val Ala Ser Val Ser Thr Ala Ser Val Ala Thr Ser 
                1445                1450                1455 

Ser Phe Pro Glu Pro Thr Thr Asp Asp Val Ser Pro Ser Leu His Ala 
            1460                1465                1470 

Glu Val Gly Ser Pro His Ser Thr Glu Val Asp Asp Ser Leu Ser Val 
        1475                1480                1485 

Ser Val Val Gln Thr Pro Thr Thr Phe Gln Glu Thr Glu Met Ser Pro 
    1490                1495                1500 

Ser Lys Glu Glu Cys Pro Arg Pro Met Ser Ile Ser Pro Pro Asp Phe 
1505                1510                1515                1520 

Ser Pro Lys Thr Ala Lys Ser Arg Thr Pro Val Gln Asp His Arg Ser 
                1525                1530                1535 

Glu Gln Ser Ser Met Ser Ile Glu Phe Gly Gln Glu Ser Pro Glu His 
            1540                1545                1550 

Ser Leu Ala Met Asp Phe Ser Arg Gln Ser Pro Asp His Pro Thr Val 
        1555                1560                1565 

Gly Ala Gly Met Leu His Ile Thr Glu Asn Gly Pro Thr Glu Val Asp 
    1570                1575                1580 

Tyr Ser Pro Ser Asp Ile Gln Asp Ser Ser Leu Ser His Lys Ile Pro 
1585                1590                1595                1600 

Pro Thr Glu Glu Pro Ser Tyr Thr Gln Asp Asn Asp Leu Ser Glu Leu 
                1605                1610                1615 

Ile Ser Val Ser Gln Val Glu Ala Ser Pro Ser Thr Ser Ser Ala His 
            1620                1625                1630 

Thr Pro Ser Gln Ile Ala Ser Pro Leu Gln Glu Asp Thr Leu Ser Asp 
        1635                1640                1645 

Val Val Pro Pro Arg Asp Met Ser Leu Tyr Ala Ser Leu Ala Ser Glu 
    1650                1655                1660 

Lys Val Gln Ser Leu Glu Gly Glu Lys Leu Ser Pro Lys Ser Asp Ile 
1665                1670                1675                1680 

Ser Pro Leu Thr Pro Arg Glu Ser Ser Pro Thr Tyr Ser Pro Gly Phe 
                1685                1690                1695 

Ser Asp Ser Thr Ser Gly Ala Lys Glu Ser Thr Ala Ala Tyr Gln Thr 
            1700                1705                1710 

Ser Ser Ser Pro Pro Ile Asp Ala Ala Ala Ala Glu Pro Tyr Gly Phe 
        1715                1720                1725 

Arg Ser Ser Met Leu Phe Asp Thr Met Gln His His Leu Ala Leu Ser 
    1730                1735                1740 

Arg Asp Leu Thr Thr Ser Ser Val Glu Lys Asp Asn Gly Gly Lys Thr 
1745                1750                1755                1760 

Pro Gly Asp Phe Asn Tyr Ala Tyr Gln Lys Pro Glu Ser Thr Thr Glu 
                1765                1770                1775 

Ser Pro Asp Glu Glu Asp Tyr Asp Tyr Glu Ser His Glu Lys Thr Ile 
            1780                1785                1790 

Gln Ala His Asp Val Gly Gly Tyr Tyr Tyr Glu Lys Thr Glu Arg Thr 
        1795                1800                1805 

Ile Lys Ser Pro Cys Asp Ser Gly Tyr Ser Tyr Glu Thr Ile Glu Lys 
    1810                1815                1820 

Thr Thr Lys Thr Pro Glu Asp Gly Gly Tyr Ser Cys Glu Ile Thr Glu 
1825                1830                1835                1840 

Lys Thr Thr Arg Thr Pro Glu Glu Gly Gly Tyr Ser Tyr Glu Ile Ser 
                1845                1850                1855 

Glu Lys Thr Thr Arg Thr Pro Glu Val Ser Gly Tyr Thr Tyr Glu Lys 
            1860                1865                1870 

Thr Glu Arg Ser Arg Arg Leu Leu Asp Asp Ile Ser Asn Gly Tyr Asp 
        1875                1880                1885 

Asp Thr Glu Asp Gly Gly His Thr Leu Gly Asp Cys Ser Tyr Ser Tyr 
    1890                1895                1900 

Glu Thr Thr Glu Lys Ile Thr Ser Phe Pro Glu Ser Glu Ser Tyr Ser 
1905                1910                1915                1920 

Tyr Glu Thr Thr Thr Lys Thr Thr Arg Ser Pro Asp Thr Ser Ala Tyr 
                1925                1930                1935 

Cys Tyr Glu Thr Met Glu Lys Ile Thr Lys Thr Pro Gln Ala Ser Thr 
            1940                1945                1950 

Tyr Ser Tyr Glu Thr Ser Asp Arg Cys Tyr Thr Pro Glu Arg Lys Ser 
        1955                1960                1965 

Pro Ser Glu Ala Arg Gln Asp Val Asp Leu Cys Leu Val Ser Ser Cys 
    1970                1975                1980 

Glu Phe Lys His Pro Lys Thr Glu Leu Ser Pro Ser Phe Ile Asn Pro 
1985                1990                1995                2000 

Asn Pro Leu Glu Trp Phe Ala Gly Glu Glu Pro Thr Glu Glu Ser Glu 
                2005                2010                2015 

Arg Pro Leu Thr Gln Ser Gly Gly Ala Pro Pro Pro Ser Gly Gly Lys 
            2020                2025                2030 

Gln Gln Gly Arg Gln Cys Asp Glu Thr Pro Pro Thr Ser Val Ser Glu 
        2035                2040                2045 

Ser Ala Pro Ser Gln Thr Asp Ser Asp Val Pro Pro Glu Thr Glu Glu 
    2050                2055                2060 

Cys Pro Ser Ile Thr Ala Asp Ala Asn Leu Asp Ser Glu Asp Glu Ser 
2065                2070                2075                2080 

Glu Thr Ile Pro Thr Asp Lys Thr Val Thr Tyr Lys His Met Asp Pro 
                2085                2090                2095 

Pro Pro Ala Pro Met Gln Asp Arg Ser Pro Ser Pro Arg His Pro Asp 
            2100                2105                2110 

Val Ser Met Val Asp Pro Glu Ala Leu Ala Ile Glu Gln Asn Leu Gly 
        2115                2120                2125 

Lys Ala Leu Lys Lys Asp Leu Lys Glu Lys Ala Lys Thr Lys Lys Pro 
    2130                2135                2140 

Gly Thr Lys Thr Lys Ser Ser Ser Pro Val Lys Lys Gly Asp Gly Lys 
2145                2150                2155                2160 

Ser Lys Pro Ser Ala Ala Ser Pro Lys Pro Gly Ala Leu Lys Glu Ser 
                2165                2170                2175 

Ser Asp Lys Val Ser Arg Val Ala Ser Pro Lys Lys Lys Glu Ser Val 
            2180                2185                2190 

Glu Lys Ala Met Lys Thr Thr Thr Thr Pro Glu Val Lys Ala Thr Arg 
        2195                2200                2205 

Gly Glu Glu Lys Asp Lys Glu Thr Lys Asn Ala Ala Asn Ala Ser Ala 
    2210                2215                2220 

Ser Lys Ser Val Lys Thr Ala Thr Ala Gly Pro Gly Thr Thr Lys Thr 
2225                2230                2235                2240 

Ala Lys Ser Ser Thr Val Pro Pro Gly Leu Pro Val Tyr Leu Asp Leu 
                2245                2250                2255 

Cys Tyr Ile Pro Asn His Ser Asn Ser Lys Asn Val Asp Val Glu Phe 
            2260                2265                2270 

Phe Lys Arg Val Arg Ser Ser Tyr Tyr Val Val Ser Gly Asn Asp Pro 
        2275                2280                2285 

Ala Ala Glu Glu Pro Ser Arg Ala Val Leu Asp Ala Leu Leu Glu Gly 
    2290                2295                2300 

Lys Ala Gln Trp Gly Ser Asn Met Gln Val Thr Leu Ile Pro Thr His 
2305                2310                2315                2320 

Asp Ser Glu Val Met Arg Glu Trp Tyr Gln Glu Thr His Glu Lys Gln 
                2325                2330                2335 

Gln Asp Leu Asn Ile Met Val Leu Ala Ser Ser Ser Thr Val Val Met 
            2340                2345                2350 

Gln Asp Glu Ser Phe Pro Ala Cys Lys Ile Glu Leu 
        2355                2360 

 
           
             67  
             7095  
             DNA  
             Rattus norvegicus  
             
               Microtubule associated protein IB  
             
           
            67 

ggacaaaaga tcctccatca ccgaagtgac gtcttagaaa ctgtagttct gatcaaccct     60 

tcggatgaag cagtcagcac cgaggtgcgt ttgatgatca ctgacgccgc ccgccataaa    120 

ctgctggtgc tcaccggaca gtgctttgag aacactggag agctcatcct ccagtcaggc    180 

tctttctcct tccagaactt catagagatt ttcaccgacc aagagattgg ggagctccta    240 

agcaccaccc atcctgccaa caaagccagc ctcaccctct tctgccctga ggaaggagac    300 

tggaagaact ccaaccttga cagacacaat ctccaagact tcatcaacat caagctcaac    360 

tcagcttcta tcttgccaga aatggaggga ctttctgagt tcaccgagta cctctcggag    420 

tctgtcgaag tcccctcccc ctttgacatc ctggagcccc cgacctcggg cggatttctg    480 

aagctctcca agccttgttg ttacattttt ccggggggcc gcggggactc tgccctgttc    540 

gcagtgaacg gattcaacat gctcattaac ggaggatcag aaagaaagtc ctgcttctgg    600 

aagctcattc ggcacttgga ccgggtggac tccatcctgc tcacccacat tggggatgac    660 

aacttgcccg ggatcaacag catgttgcaa cgcaagattg cagagctgga agaggagtcc    720 

cagggctcca ccagcaacag tgactggatg aaaaacctca tctcccctga cttgggggtt    780 

gtgtttctca atgtacctga aaatctgaaa aacccagaac ccaacatcaa gatgaagaga    840 

agtacagaag aagcatgctt caccctccag tacctaaaca aactgtccat gaaaccagag    900 

cctttattta gaagtgtagg caatgccatt gagcctgtca tcctgttcca aaaaatggga    960 

gtgggtaaac tgaagatgta tgtgcttaac ccagtcaaaa gcagcaagga aatgcagtat   1020 

ttcatgcagc agtggactgg aaccaacaaa gacaaggctg aacttatcct gcccaatggt   1080 

caagaagtag acatcccgat ttcctaccta gcttccgtct cgtctttgat tgtgtggcac   1140 

ccagccaacc ctgctgagaa aatcatccgg gttctgtttc ctggaaacag cacccagtac   1200 

aacatcctag aagggctgga aaaactcaaa catctagact tcctaaagca gccactggcc   1260 

acccaaaaag atctcactgg ccaggtgtcc acccccccag tgaaacaggt caagttgaaa   1320 

cagcgggctg acagccgaga gagtctgaag ccagccacaa aaccactttc cagtaaatca   1380 

gtgaggaagg agtccaaaga ggaggcccct gaagccacaa aagccagcca agtggaaaaa   1440 

acacccaaag ttgaaagcaa agagaaagtg atagtgaaaa aagacaagcc aggaaaggta   1500 

gaaagtaagc catcggtgac ggaaaaggag gtgcccagca aagaggagca gtcgcccgtc   1560 

aaagctgagg tggctgagaa ggcggccacg gagagcaaac ccaaagtcac caaagacaaa   1620 

gtggtaaaaa aggaaataaa gacaaaaccc gaagaaaaga aagaggagaa gcccaagaag   1680 

gaagtggcta aaaaggaaga caaaactccc ctcaagaaag acgagaagcc caaaaaggaa   1740 

gaggcgaaga aggagatcaa gaaagaaatc aaaaaggaag agaaaaagga gctgaagaaa   1800 

gaggtgaaga aggaaacgcc cctgaaggac gccaagaagg aggtgaagaa agacgagaag   1860 

aaagaagtta aaaaggaaga gaaggaaccc aaaaaggaga ttaagaagat ctccaaggac   1920 

ataaagaaat ccactcctct gtcagacaca aagaaaccgg ctgcattgaa accaaaagta   1980 

gcaaagaaag aagagcccac caagaaggag cctattgctg ctgggaaact caaggacaag   2040 

gggaaggtca aagtcattaa aaaggaaggc aagaccacag aggccgctgc cacagctgtt   2100 

ggcactgctg ccgtggctgc agcagccgga gtagcggcca gcggtcctgc caaagaactt   2160 

gaagctgagc ggtccctcat gtcgtcccct gaggatctaa ccaaggactt tgaggagcta   2220 

aaggctgagg agatcgatgt agcgaaggac atcaagcctc agctggagct cattgaagat   2280 

gaagagaaac tgaaggaaac cgagccggga gaagcctacg tcattcagaa agagacggaa   2340 

gtcagcaaag gttctgctga gtcacctgat gaagggatca ccaccactga gggggaaggg   2400 

gagtgcgagc aaacccccga ggagctggag ccagttgaga agcagggcgt ggatgacatc   2460 

gagaagttcg aggatgaagg cgctggtttt gaagaatcct cagaggccgg agactacgaa   2520 

gagaaggcag aaactgagga ggccgaggag ccggaagaag acggggaaga caatgtgagc   2580 

gggagcgcct cgaagcacag ccccacagaa gacgaagaaa tcgctaaggc tgaggcggac   2640 

gtacacatca aggagaagag ggagtctgtg gccagcggcg atgaccgggc cgaagaagac   2700 

atggatgaag cgcttgagaa aggagaagct gaacagtctg aggaggaggg tgaggaggag   2760 

gaggacaaag cagaggacgc cagagaggaa gaccatgagc ccgacaaaac tgaggctgaa   2820 

gattatgtga tggctgtggt tgacaaggcc gcggaggccg gagtcaccga ggatcagtat   2880 

gatttcctgg ggacaccggc caagcaacct ggagtccagt ctcctagccg agaacccgcg   2940 

tcttcaattc atgatgagac cctacccgga ggctccgaga gcgaggccac tgcttcagat   3000 

gaggagaatc gagaagacca gcctgaggaa ttcactgcta cctccggata tactcagtcc   3060 

accatcgaga tatctagtga gccgactcca atggatgaga tgtccactcc tcgagatgtg   3120 

atgaccgacg agaccaacaa tgaggagaca gagtccccgt ctcaggagtt cgtgaacatt   3180 

accaaatacg agtcttcgct gtactctcag gagtactcca aacctgtggt tgcatcattc   3240 

aatggattgt cagacgggtc aaagacagac gccactgacg gtagggatta caacgcttcc   3300 

gcctccacca tatcaccacc ttcgtccatg gaagaagaca aattcagcaa gtctgctctt   3360 

cgtgacgctt accgcccaga agagacggac gtgaaaaccg gtgccgagtt ggacatcaaa   3420 

gatgtttcgg atgagagact tagcccagcc aagagtccat ccctgagtcc ttctccacca   3480 

tcacccatag agaagactcc cctgggtgaa cgtagcgtga atttctctct gacacccaac   3540 

gagatcaaag cctctgcaga gggagaggca acagcagtag tgtcccccgg agtgacccaa   3600 

gcagtagttg aagaacactg tgccagtcct gaggagaaga ccttggaggt agtgtcaccg   3660 

tctcagtctg tgacaggcag tgcgggccac acaccttact accaatctcc caccgacgaa   3720 

aagtccagtc acctacctac agaagtcact gagaacgcgc aggcagtccc ggtgagcttt   3780 

gaattcactg aggccaaaga tgagaacgag aggtcgtcca tcagccccat ggatgaacct   3840 

gtgcctgact cagagtctcc tatcgagaaa gttctgtctc cgttacgcag ccctcccctt   3900 

attggatccg agtccgcata tgaagacttc ctgagtgcgg atgacaaggc tcttggcaga   3960 

cgttcagaaa gcccctttga agggaagaat ggaaagcaag gcttctcaga caaagaaagc   4020 

ccagtttctg acctgacttc cgatctttac caagacaagc aggaagagaa aagggcgggc   4080 

ttcataccga taaaggaaga ctttagtcca gaaaagaaag ccagcgatgc tgaaatcatg   4140 

agttctcaat cagctctggc tttggatgaa aggaaactgg gaggagatgg atctccaacg   4200 

caagtagatg tcagtcagtt tggctctttc aaagaagaca ccaagatgtc catttcggaa   4260 

ggcaccgttt cagacaagtc cgccacgcct gtggatgagg gcgcggaaga cacctattca   4320 

cacatggaag gtgtggcctc agtgtcaacg gcctctgtgg ctaccagctc gtttccagag   4380 

ccaaccacag atgacgtgtc tccttctctc cacgctgaag tgggctctcc acattccaca   4440 

gaggtggatg actccctgtc ggtgtcggtg gtgcaaacac caactacttt ccaggaaaca   4500 

gaaatgtctc cgtctaaaga agagtgccca agaccaatgt cgatttctcc tcctgacttc   4560 

tcccctaaga cagccaaatc caggacacca gttcaagatc accgatccga acagtcttca   4620 

atgtctattg aattcggtca ggaatccccc gagcattctc ttgctatgga ctttagtcgg   4680 

cagtctccag accaccctac tgtgggtgct ggtatgcttc acatcaccga aaatgggcca   4740 

actgaggtgg actacagtcc ctccgatatc caggactcta gtttgtcaca taagattcct   4800 

cctacagaag agccatccta cacccaggat aatgatctgt ccgagctcat ctctgtgtct   4860 

caggtggagg cttccccatc cacctcttct gctcacactc cttctcagat agcctctcct   4920 

cttcaggaag acactctctc tgatgtcgtt cctcccagag atatgtcctt atatgcctcg   4980 

cttgcgtctg agaaagtgca gagcctggaa ggagagaaac tctctccaaa atccgatatt   5040 

tctccgctca cccctcgaga gtcctcacct acatattcac ctggcttttc agattctacc   5100 

tctggagcta aagagagtac agcggcttac caaacctcct cttccccacc aatagatgca   5160 

gcagccgcag agccctacgg cttccgctcc tcaatgttat ttgatacaat gcagcatcac   5220 

ctggccttga gtagagattt gaccacatct agtgtggaga aggacaatgg agggaagaca   5280 

cccggtgact ttaactatgc ctatcaaaag cccgagagca ccaccgaatc cccagatgaa   5340 

gaagattatg actatgaatc tcacgagaaa accatccagg cccacgatgt gggtggttac   5400 

tactatgaga agacagagag aaccataaaa tccccatgtg acagtggata ctcctatgag   5460 

accattgaga agaccaccaa gaccccagaa gatggtggct actcctgtga aattaccgag   5520 

aaaaccactc ggacccctga agagggcggg tactcgtatg agatcagcga gaagacaaca   5580 

cgaacccctg aagtaagtgg ctacacctat gagaagaccg agaggtccag aaggctcctc   5640 

gatgacatta gcaatggcta cgatgacact gaggatggtg gccacacact tggcgactgt   5700 

agctattcct acgaaaccac tgagaaaatt accagctttc ctgaatctga aagctattcc   5760 

tatgagacaa ctacaaaaac aacacggagt ccagacacct ctgcatactg ttacgagacc   5820 

atggagaaga tcaccaagac cccacaggca tccacatact cctatgagac ctcagaccga   5880 

tgctacactc cagaaaggaa gtccccctcg gaggcacgcc aggatgttga cttgtgtctg   5940 

gtgtcctcct gtgaattcaa gcatcccaag accgagctct caccttcctt cattaatcca   6000 

aaccctctcg agtggtttgc tggggaagag cccactgaag aatctgagag gcctctcact   6060 

cagtctggag gagccccccc accttcagga ggaaaacaac agggcagaca atgcgatgaa   6120 

actccaccca cctcagtcag tgagtcagct ccatcccaga cggactctga tgttccccca   6180 

gagacagaag agtgcccctc catcacagct gatgccaacc ttgactctga agatgagtca   6240 

gaaaccatcc ccacagacaa aacggttacg tacaaacaca tggacccgcc tccagccccc   6300 

atgcaagacc gaagcccttc tcctcgccac cctgatgtgt ccatggtgga tccagaggcc   6360 

ttggctattg agcagaacct aggcaaggct ctgaaaaagg atctgaagga gaaggccaag   6420 

accaagaaac caggcacaaa gaccaagtcc tcttcacctg tcaaaaaggg tgatgggaag   6480 

tccaagcctt cagcagcttc ccccaaacca ggagccttga aggaatcctc tgacaaggtg   6540 

tccagagtgg cttctcccaa gaagaaagag tctgtggaga aagctatgaa gaccaccacc   6600 

actcctgagg tcaaagccac acgaggggaa gagaaggaca aggaaactaa gaatgcagcc   6660 

aatgcttctg catccaagtc agtgaagact gcaacagcag gaccaggaac cactaagacg   6720 

gccaagtcgt ccaccgtgcc tcccggcctc cctgtgtatt tggacctctg ctatattccc   6780 

aaccacagca acagtaagaa tgtcgatgtt gagtttttca agagagtgag gtcatcttac   6840 

tacgtggtga gtgggaacga ccctgccgcg gaggagccca gccgggctgt cctggatgcc   6900 

ttgttggaag ggaaggctca gtggggaagc aacatgcagg tgactctgat cccaacacat   6960 

gactctgagg tgatgaggga gtggtaccag gagacccacg agaagcagca agacctcaac   7020 

atcatggtcc tagcaagcag tagtacagtg gtcatgcaag acgagtcctt ccctgcatgc   7080 

aagatagaac tgtag                                                    7095 

 
           
             68  
             379  
             PRT  
             Rattus norvegicus  
             
               cdc 37 homolog  
             
           
            68 

Met Val Asp Tyr Ser Val Trp Asp His Ile Glu Val Ser Asp Asp Glu 
1               5                   10                  15 

Asp Glu Thr His Pro Asn Ile Asp Thr Ala Ser Leu Phe Arg Trp Arg 
            20                  25                  30 

His Gln Ala Arg Val Glu Arg Met Glu Gln Phe Gln Lys Glu Lys Glu 
        35                  40                  45 

Glu Leu Asp Arg Gly Cys Arg Glu Cys Lys Arg Lys Val Ala Glu Phe 
    50                  55                  60 

Gln Arg Lys Leu Lys Glu Leu Glu Val Ala Glu Gly Gly Gly Gln Val 
65                  70                  75                  80 

Glu Leu Glu Arg Leu Arg Ala Glu Ala Gln Gln Leu Arg Lys Glu Glu 
                85                  90                  95 

Arg Thr Gly Ser Arg Ser Trp Arg Thr Cys Gly Lys Lys Glu Lys Asn 
            100                 105                 110 

Met Pro Trp Asn Val Asp Thr Leu Ser Lys Asp Gly Phe Ser Lys Ser 
        115                 120                 125 

Met Val Asn Thr Lys Pro Glu Lys Ala Glu Glu Asp Ser Glu Glu Ala 
    130                 135                 140 

Arg Glu Gln Lys His Lys Thr Phe Val Glu Lys Tyr Glu Lys Gln Ile 
145                 150                 155                 160 

Lys His Phe Gly Met Leu His Arg Trp Asp Asp Ser Gln Lys Tyr Leu 
                165                 170                 175 

Ser Asp Asn Val His Leu Val Cys Glu Glu Thr Ala Asn Tyr Leu Val 
            180                 185                 190 

Ile Trp Cys Ile Asp Leu Glu Val Glu Glu Lys Cys Ala Leu Met Glu 
        195                 200                 205 

Gln Val Ala His Gln Thr Met Val Met Gln Phe Ile Leu Glu Leu Ala 
    210                 215                 220 

Lys Ser Leu Lys Val Asp Pro Arg Ala Cys Phe Arg Gln Phe Phe Thr 
225                 230                 235                 240 

Lys Ile Lys Thr Ala Asp Gln Gln Tyr Met Glu Gly Phe Lys Tyr Glu 
                245                 250                 255 

Leu Glu Ala Phe Lys Glu Arg Val Arg Gly Arg Ala Lys Leu Arg Ile 
            260                 265                 270 

Glu Lys Ala Met Lys Glu Tyr Glu Glu Glu Glu Arg Lys Lys Arg Leu 
        275                 280                 285 

Gly Pro Gly Gly Leu Asp Pro Val Glu Val Tyr Glu Ser Leu Pro Glu 
    290                 295                 300 

Glu Leu Gln Lys Cys Phe Asp Val Lys Asp Val Gln Met Leu Gln Asp 
305                 310                 315                 320 

Ala Ile Ser Lys Met Asp Pro Thr Asp Ala Lys Tyr His Met Gln Arg 
                325                 330                 335 

Cys Ile Asp Ser Gly Leu Trp Val Pro Asn Ser Lys Ser Gly Glu Ala 
            340                 345                 350 

Lys Glu Gly Glu Glu Ala Gly Pro Gly Asp Pro Leu Leu Glu Ala Val 
        355                 360                 365 

Pro Lys Ala Gly Phe Glu Lys Asp Ile Ser Ala 
    370                 375 

 
           
             69  
             1604  
             DNA  
             Rattus norvegicus  
             
               cdc 37 homolog  
             
           
            69 

cgagcggact ctctagccct tgtgggtccc tgttcagcgc ccggccgtgt ggagacccgg     60 

gagccagtcc gggcctccga ggcgaagatg gtggactaca gcgtttggga tcacatcgag    120 

gtgtcggacg atgaggacga gacgcacccc aacatagaca cggccagcct gttccgctgg    180 

cggcaccagg cccgggtgga acgcatggaa cagtttcaga aggagaaaga agaactggac    240 

cggggctgcc gggaatgcaa gcgcaaggta gccgagttcc agcgcaagct gaaggaactg    300 

gaagtggctg agggtggtgg ccaggtggag cttgagcggc ttcgagcgga ggcacagcag    360 

ctgcgcaagg aggagcgaac tgggagcaga agctggagga catgcggcaa aaaggagaag    420 

aacatgccct ggaatgtgga cacactcagc aaagatggct tcagcaagag catggtcaat    480 

accaagcctg agaaggcaga ggaagactca gaggaggcaa gggagcagaa acacaagacc    540 

ttcgttgaaa aatacgagaa acagatcaaa catttcggca tgctccaccg ctgggatgac    600 

agccagaagt acctgtcaga caatgtccac ctggtgtgcg aggagacagc caactacctg    660 

gttatctggt gcattgacct ggaagtcgag gagaagtgtg ccctgatgga gcaggtggct    720 

caccagacca tggtcatgca gttcatcctg gagttggcca agagtctgaa ggtcgacccc    780 

cgggcctgct tccggcagtt tttcaccaag atcaagactg ctgaccagca gtacatggag    840 

ggcttcaagt atgaactgga ggcctttaag gagcgtgtgc ggggccgcgc caagctgcga    900 

atagagaagg ccatgaagga gtacgaggag gaggagcgca agaagaggct aggccctggt    960 

ggcctggacc ctgtggaggt ctacgaatct ctgcctgagg agctgcagaa gtgctttgat   1020 

gtgaaggatg tacagatgct gcaagacgcc atcagcaaga tggatcctac tgatgccaag   1080 

tatcacatgc agcgttgcat cgattctggc ctctgggtcc ccaactccaa gtctggtgag   1140 

gccaaggagg gggaggaggc gggccccggg gacccattgc tggaagccgt ccccaaagcg   1200 

ggcttcgaga aagacatcag tgcgtgaccc ttctccagaa aaggaaaccc agccaccatg   1260 

tccctagctc ccgacttctt cacttctgct gctcctcacg tcctggccca tggcctgccc   1320 

catgtcccct ctcctccggc ctgtccccag cactcatcag attctctgct tcgggttgag   1380 

gggctctgct gtctgcagcc cccccacccc catcagcgtt atgccaaagt cctgggggtc   1440 

tggggagggg cagcagctgc caggttggtc caccgggttg gggatacaca tgtccccatc   1500 

tgggcagggt ctgtccctat gggtctatgg tcactgtttc tcttctgtct agctttctgt   1560 

cctgcaactg gcaacaatgt ccaataaaca gttccaggtg ctgg                    1604 

 
           
             70  
             216  
             PRT  
             Homo sapiens  
             
               Rab5c-like protein  
             
           
            70 

Met Ala Gly Arg Gly Gly Ala Arg Arg Pro Asn Gly Pro Ala Ala Gly 
1               5                   10                  15 

Asn Lys Ile Cys Gln Phe Lys Leu Val Leu Leu Gly Glu Ser Ala Val 
            20                  25                  30 

Gly Lys Ser Ser Leu Val Leu Arg Phe Val Lys Gly Gln Phe His Glu 
        35                  40                  45 

Tyr Gln Glu Ser Thr Ile Gly Ala Ala Phe Leu Thr Gln Thr Val Cys 
    50                  55                  60 

Leu Asp Asp Thr Thr Val Lys Phe Glu Ile Trp Asp Thr Ala Gly Gln 
65                  70                  75                  80 

Glu Arg Tyr His Ser Leu Ala Pro Met Tyr Tyr Arg Gly Ala Gln Ala 
                85                  90                  95 

Ala Ile Val Val Tyr Asp Ile Thr Asn Thr Asp Thr Phe Ala Arg Ala 
            100                 105                 110 

Lys Asn Trp Val Lys Glu Leu Gln Arg Gln Ala Ser Pro Asn Ile Val 
        115                 120                 125 

Ile Ala Leu Ala Gly Asn Lys Ala Asp Leu Ala Ser Lys Arg Ala Val 
    130                 135                 140 

Glu Phe Gln Glu Ala Gln Ala Tyr Ala Asp Asp Asn Ser Leu Leu Phe 
145                 150                 155                 160 

Met Glu Thr Ser Ala Lys Thr Ala Met Asn Val Asn Glu Ile Phe Met 
                165                 170                 175 

Ala Ile Ala Lys Lys Leu Pro Lys Asn Glu Pro Gln Asn Ala Thr Gly 
            180                 185                 190 

Ala Pro Gly Arg Asn Arg Gly Val Asp Leu Gln Glu Asn Asn Pro Ala 
        195                 200                 205 

Ser Arg Ser Gln Cys Cys Ser Asn 
    210                 215 

 
           
             71  
             1529  
             DNA  
             Homo sapiens  
             
               Rab5c-like protein  
             
           
            71 

cttacactaa gtgcctcttt gcatagcacc agtccccacc cgcacgctct ctggaccact     60 

acagctggac gggcaatggc gggtcgggga ggcgcacgac gacccaatgg accagctgct    120 

gggaacaaga tctgtcaatt taagctggtt ctgctggggg agtctgcggt aggcaaatcc    180 

agcctcgtcc tccgctttgt caagggacag tttcacgagt accaggagag cacaattgga    240 

gcggccttcc tcacacagac tgtctgcctg gatgacacaa cagtcaagtt tgagatctgg    300 

gacacagctg gacaggagcg gtatcacagc ctggccccca tgtactatcg gggggcccag    360 

gctgccatcg tggtctatga catcaccaac acagatacat ttgcacgggc caagaactgg    420 

gtgaaggagc tacagaggca ggccagcccc aacatcgtca ttgcactcgc gggtaacaag    480 

gcagacctgg ccagcaagag agccgtggaa ttccaggaag cacaagccta tgcagacgac    540 

aacagtttgc tgttcatgga gacatcagca aagactgcaa tgaacgtgaa cgaaatcttc    600 

atggcaatag ctaagaagct tcccaagaac gagccccaga atgcaactgg tgctccaggc    660 

cgaaaccgag gtgtggacct ccaggagaac aacccagcca gccggagcca gtgctgcagc    720 

aactgagccc cccttgcctg cccgctgccc ccgcctcctc cgcctgaatg acccgactgg    780 

aatccactct aaccaatcgc acttaacgac tcgggccacc actggggggg cagggggagg    840 

ggtccaccat gatttctcca tataattttg atcataggcc ggagtgagtc attccacctg    900 

cacctttctg tacaaatact aattcaattt taagtcttaa gtcacttttt taatatatat    960 

gatcttctgc tcttcccact tcctcccctt tctactgctc tcccattttc ccttgctggg   1020 

agtagccaca tgctcttgcc ccccaaccct tgtatatggg gacagtgggg tcagtgcagc   1080 

taccctttct ttccctctgc ggaacagcgg acccagcaag agcatccaca tcctcacttt   1140 

gttcggagtg gtctttggtt tgggcggtgg ggcagacctt gggaaggggc ttaggaaggg   1200 

agaggcagct cttccttcag ctggctctca tcaggctgca gccccctccc cgctcccacc   1260 

tccctgctgg gaaaccacag cattatcaca gcattattgt gacagccacg aacccattgc   1320 

ccacaacccc tccaccctcg gtcaccccaa cctctggctc tgagccctgt tctgaccaaa   1380 

tcatgatgat gagtatttgg gggtgggtgg gtaagggggg gagtgggagg ggacggaacc   1440 

aactttttct gtattttgta ttgtatgttt tcttcaacat gtaaccaatc agtatcttgt   1500 

caatatagtc agccgatcga tcgacctca                                     1529 

 
           
             72  
             731  
             PRT  
             Mus musculus  
             
               Gelsolin  
             
           
            72 

Met Val Val Glu His Pro Glu Phe Leu Lys Ala Gly Lys Glu Pro Gly 
1               5                   10                  15 

Leu Gln Ile Trp Arg Val Glu Lys Phe Asp Leu Val Pro Val Pro Pro 
            20                  25                  30 

Asn Leu Tyr Gly Asp Phe Phe Thr Gly Asp Ala Tyr Val Ile Leu Lys 
        35                  40                  45 

Thr Val Gln Leu Arg Asn Gly Asn Leu Gln Tyr Asp Leu His Tyr Trp 
    50                  55                  60 

Leu Gly Asn Glu Cys Ser Gln Asp Glu Ser Gly Ala Ala Ala Ile Phe 
65                  70                  75                  80 

Thr Val Gln Leu Asp Asp Tyr Leu Asn Gly Arg Ala Val Gln His Arg 
                85                  90                  95 

Glu Val Gln Gly Phe Glu Ser Ser Thr Phe Ser Gly Tyr Phe Lys Ser 
            100                 105                 110 

Gly Leu Lys Tyr Lys Lys Gly Gly Val Ala Ser Gly Phe Lys His Val 
        115                 120                 125 

Val Pro Asn Glu Val Val Val Gln Arg Leu Phe Gln Val Lys Gly Arg 
    130                 135                 140 

Arg Val Val Arg Ala Thr Glu Val Pro Val Ser Trp Asp Ser Phe Asn 
145                 150                 155                 160 

Asn Gly Asp Cys Phe Ile Leu Asp Leu Gly Asn Asn Ile Tyr Gln Trp 
                165                 170                 175 

Cys Gly Ser Gly Ser Asn Lys Phe Glu Arg Leu Lys Ala Thr Gln Val 
            180                 185                 190 

Ser Lys Gly Ile Arg Asp Asn Glu Arg Ser Gly Arg Ala Gln Val His 
        195                 200                 205 

Val Ser Glu Glu Glu Thr Glu Pro Glu Ala Met Leu Gln Val Leu Gly 
    210                 215                 220 

Pro Lys Pro Ala Leu Pro Glu Gly Thr Glu Asp Thr Ala Lys Glu Asp 
225                 230                 235                 240 

Ala Ala Asn Arg Lys Leu Ala Lys Leu Tyr Lys Val Ser Asn Gly Ala 
                245                 250                 255 

Gly Ser Met Ser Val Ser Leu Val Ala Asp Glu Asn Pro Phe Ala Gln 
            260                 265                 270 

Gly Pro Leu Arg Ser Glu Asp Cys Phe Ile Leu Asp His Gly Arg Asp 
        275                 280                 285 

Gly Lys Ile Phe Val Trp Lys Gly Lys Gln Ala Asn Met Glu Glu Arg 
    290                 295                 300 

Lys Ala Ala Leu Lys Thr Ala Ser Asp Phe Ile Ser Lys Met Gln Tyr 
305                 310                 315                 320 

Pro Arg Gln Thr Gln Val Ser Val Leu Pro Glu Gly Gly Glu Thr Pro 
                325                 330                 335 

Leu Phe Lys Gln Phe Phe Lys Asn Trp Arg Asp Pro Asp Gln Thr Asp 
            340                 345                 350 

Gly Pro Gly Leu Gly Tyr Leu Ser Ser His Ile Ala Asn Val Glu Arg 
        355                 360                 365 

Val Pro Phe Asp Ala Gly Thr Leu His Thr Ser Thr Ala Met Ala Ala 
    370                 375                 380 

Gln His Gly Met Asp Asp Asp Gly Thr Gly Gln Lys Gln Ile Trp Arg 
385                 390                 395                 400 

Ile Glu Gly Ser Asn Lys Val Pro Val Asp Pro Ala Thr Tyr Gly Gln 
                405                 410                 415 

Phe Tyr Gly Gly Asp Ser Tyr Ile Ile Leu Tyr Asn Tyr Arg His Gly 
            420                 425                 430 

Gly Arg Gln Gly Gln Ile Ile Tyr Asn Trp Gln Gly Ala Gln Ser Thr 
        435                 440                 445 

Gln Asp Glu Val Ala Ala Ser Ala Ile Leu Thr Ala Gln Leu Asp Glu 
    450                 455                 460 

Glu Leu Gly Gly Thr Pro Val Gln Ser Arg Val Val Gln Gly Lys Glu 
465                 470                 475                 480 

Pro Ala His Leu Met Ser Leu Phe Gly Gly Lys Pro Met Ile Ile Tyr 
                485                 490                 495 

Lys Gly Gly Thr Ser Arg Asp Gly Gly Gln Thr Ala Pro Ala Ser Ile 
            500                 505                 510 

Arg Leu Phe Gln Val Arg Ala Ser Ser Ser Gly Ala Thr Arg Ala Val 
        515                 520                 525 

Glu Val Met Pro Lys Ser Gly Ala Leu Asn Ser Asn Asp Ala Phe Val 
    530                 535                 540 

Leu Lys Thr Pro Ser Ala Ala Tyr Leu Trp Val Gly Ala Gly Ala Ser 
545                 550                 555                 560 

Glu Ala Glu Lys Thr Ala Ala Gln Glu Leu Leu Lys Val Leu Arg Ser 
                565                 570                 575 

Gln His Val Gln Val Glu Glu Gly Ser Glu Pro Asp Gly Phe Trp Glu 
            580                 585                 590 

Ala Leu Gly Gly Lys Thr Ser Tyr Arg Thr Ser Pro Arg Leu Lys Asp 
        595                 600                 605 

Lys Lys Met Asp Ala His Pro Pro Arg Leu Phe Ala Cys Ser Asn Arg 
    610                 615                 620 

Ile Gly Arg Phe Val Ile Glu Glu Val Pro Gly Glu Leu Met Gln Glu 
625                 630                 635                 640 

Asp Leu Ala Thr Asp Asp Val Met Leu Leu Asp Thr Trp Asp Gln Val 
                645                 650                 655 

Phe Val Trp Val Gly Lys Asp Ser Gln Glu Glu Glu Lys Thr Glu Ala 
            660                 665                 670 

Leu Thr Ser Ala Lys Arg Tyr Ile Glu Thr Asp Pro Ala Asn Arg Asp 
        675                 680                 685 

Arg Arg Thr Pro Ile Thr Val Val Arg Gln Gly Phe Glu Pro Pro Ser 
    690                 695                 700 

Phe Val Gly Trp Phe Leu Gly Trp Asp Asn Asn Tyr Trp Ser Val Asp 
705                 710                 715                 720 

Pro Leu Asp Arg Ala Leu Ala Glu Leu Ala Ala 
                725                 730 

 
           
             73  
             2447  
             DNA  
             Mus musculus  
             
               Gelsolin  
             
           
            73 

tgagcgcggc ccagcactat ggtggtggag caccccgaat tcctgaaggc agggaaggag     60 

cctggcctgc agatctggcg tgtggagaag tttgacctgg tgcctgtgcc ccccaacctc    120 

tatggagact tcttcacggg tgatgcctat gtcatcctga agactgtgca gctgaggaat    180 

gggaatctgc agtatgacct ccactattgg ctgggcaatg aatgcagcca ggatgagagc    240 

ggggctgctg ccatctttac tgtgcaactg gatgactacc tgaacggccg ggctgtgcag    300 

caccgtgagg ttcagggctt tgagtcgtcc accttctccg gctacttcaa gtctggactt    360 

aagtacaaga aaggaggtgt ggcatctgga ttcaaacacg tggtacccaa tgaggtggtg    420 

gtccagaggc tcttccaggt caaaggacgc cgtgtagtcc gtgctactga ggtacctgtg    480 

tcctgggaca gtttcaacaa tggcgactgc ttcattctgg acctgggaaa caatatctat    540 

cagtggtgtg gctctggcag caacaaattt gaaaggctga aggccacaca ggtgtccaag    600 

ggcatccggg acaacgagag gagtggccgt gctcaagtac acgtgtctga agaggagact    660 

gagcccgagg cgatgctgca ggtgctgggc cccaagccgg ctctgcctga aggtaccgag    720 

gacacagcca aggaagatgc agccaaccgc aagctggcca agctctacaa ggtctccaac    780 

ggtgcaggta gcatgtcagt ctccctagtg gctgatgaga accccttcgc ccaggggccc    840 

ctgagatctg aggactgctt catcctggac catggcagag atgggaaaat ctttgtttgg    900 

aaaggcaagc aggccaacat ggaggagcgg aaggctgccc tcaaaacagc ctctgacttc    960 

atctccaaga tgcagtaccc caggcagacc caggtttcag ttctcccaga gggcggtgag   1020 

acccctctct ttaagcagtt cttcaagaac tggcgggacc cagaccagac agatggcccc   1080 

ggcctgggct acctctccag ccacattgcc aacgtggagc gcgtaccttt cgatgccggc   1140 

acgctgcaca cctccaccgc catggccgct cagcacggca tggatgatga tggaactggc   1200 

cagaaacaga tctggagaat tgaaggttcc aacaaggtgc cagtggaccc tgccacatac   1260 

ggacagttct atggaggcga cagctacatc attctgtaca actaccgcca cggtggccgc   1320 

cagggacaga tcatctacaa ctggcagggt gctcagtcta cccaggatga ggttgctgct   1380 

tctgccatcc tgactgccca gctggatgag gagctgggag gaactcctgt ccagagccga   1440 

gtggtccaag gcaaagagcc tgcacacctc atgagcttgt ttggcgggaa gcccatgatc   1500 

atctacaagg gtggcacctc ccgtgatggt gggcagacag ctcctgccag tatccgcctc   1560 

ttccaagtgc gtgccagcag ctctggagcc accagggctg tggaggtgat gcctaagtct   1620 

ggtgctctga actccaacga tgcctttgtg ctgaaaaccc cctccgctgc ctacctgtgg   1680 

gtgggcgcag gagccagtga ggcagagaag acggcggccc aggagcttct gaaggtcctt   1740 

cggtcccagc atgtgcaggt ggaagaaggc agtgagccag atggcttctg ggaggctctg   1800 

ggcgggaaga cgtcctaccg cacatccccc aggcttaagg acaagaagat ggatgcccat   1860 

cctcctcgac tctttgcctg ctccaacagg atcggacgct ttgtgatcga agaggttcct   1920 

ggcgagctta tgcaggaaga cctggctact gatgacgtca tgctcctgga cacctgggac   1980 

caggtctttg tctgggttgg aaaagactcc caggaagaag aaaagacgga agccttgact   2040 

tctgctaagc ggtacatcga gacagatcca gcaaatcggg acaggcggac ccccatcaca   2100 

gtcgttaggc agggctttga gcctccttcc ttcgtgggct ggttcctcgg ctgggacaac   2160 

aactactggt cggtggatcc tttggaccgg gccttggctg agctggctgc ctgagtaagg   2220 

accaagccat caatgtcacc aatcagtgcc tttgagggtt gtccatctcc caaagacatc   2280 

atatggcaag caggaaaact atgatgtgtg cgcgcgtgtt tttgtttttg ttttttacgg   2340 

tagccaaaac aagcccttgt ggaaactcag ggtctttaca gaattgcttc aaatgtctgt   2400 

actttggaaa tgaaagccaa taaaagcttt ttgaagtgaa aaaaaaa                 2447 

 
           
             74  
             236  
             PRT  
             Rattus norvegicus  
             
               Cd81 antigen  
             
           
            74 

Met Gly Val Glu Gly Cys Thr Lys Cys Ile Lys Tyr Leu Leu Phe Val 
1               5                   10                  15 

Phe Asn Phe Val Phe Trp Leu Ala Gly Gly Val Ile Leu Gly Val Ala 
            20                  25                  30 

Leu Trp Leu Arg His Asp Pro Gln Thr Thr Thr Leu Leu Tyr Leu Glu 
        35                  40                  45 

Leu Gly Asp Lys Pro Ala Pro Ser Thr Phe Tyr Val Gly Ile Tyr Ile 
    50                  55                  60 

Leu Ile Ala Val Gly Ala Val Met Met Phe Val Gly Phe Leu Gly Cys 
65                  70                  75                  80 

Tyr Gly Ala Ile Gln Glu Ser Gln Cys Leu Leu Gly Thr Phe Phe Thr 
                85                  90                  95 

Cys Leu Val Ile Leu Phe Ala Cys Glu Val Ala Ala Gly Ile Trp Gly 
            100                 105                 110 

Phe Val Asn Lys Asp Gln Ile Ala Lys Asp Val Lys Gln Phe Tyr Asp 
        115                 120                 125 

Gln Ala Leu Gln Gln Ala Val Met Asp Asp Asp Ala Asn Asn Ala Lys 
    130                 135                 140 

Ala Val Val Lys Thr Phe His Glu Thr Leu Asn Cys Cys Gly Ser Asn 
145                 150                 155                 160 

Thr Leu Thr Thr Leu Thr Thr Ala Val Leu Arg Asn Ser Leu Cys Pro 
                165                 170                 175 

Ser Ser Ser Asn Ser Phe Thr Gln Leu Leu Lys Glu Asp Cys His Gln 
            180                 185                 190 

Lys Ile Asp Glu Leu Phe Ser Gly Lys Leu Tyr Leu Ile Gly Ile Ala 
        195                 200                 205 

Ala Ile Val Val Ala Val Ile Met Ile Phe Glu Met Ile Leu Ser Met 
    210                 215                 220 

Val Leu Cys Cys Gly Ile Arg Asn Ser Ser Val Tyr 
225                 230                 235 

 
           
             75  
             1303  
             DNA  
             Rattus norvegicus  
             
               Cd81 antigen  
             
           
            75 

gtggcggtct gagcgagcgc gcccttgctt caaagcggta gcgactctag cgcctccggc     60 

taggctccaa cccttctcta ccctacgtct caatagccac accgcagtct ccggcctcaa    120 

gcgacccagg ctatcttcca gtcccgggac tccgggtact gcggtcccca gaaccgcccg    180 

tccagggacc aacccaagtt ccgcaggccg cggcggccgc caatgggggt ggagggctgc    240 

accaaatgca tcaaatacct gctcttcgtc ttcaatttcg tcttctggct ggctggaggt    300 

gtgatcctag gtgtagctct gtggttgcgc catgatccac agaccaccac cttgctctac    360 

ctggaactgg gagacaaacc agcacctagc accttctatg tgggcatcta cattctcatt    420 

gctgtgggag ctgtgatgat gtttgtaggc ttcctggggt gctatggggc catccaggag    480 

tcccagtgcc tgctggggac gttcttcact tgccttgtga tcctgtttgc ctgtgaggta    540 

gctgcgggca tctggggctt tgtaaacaaa gaccagatcg ccaaggatgt gaagcagttc    600 

tacgaccagg cccttcagca ggctgtgatg gatgatgatg ccaacaatgc caaggcagtg    660 

gtgaagacct tccatgagac gctcaactgt tgtggctcca atacgctgac cacactgacc    720 

accgccgtgc tgaggaacag cctgtgtccc tcaagcagca actcattcac tcagcttctg    780 

aaggaagact gccatcagaa aatcgatgag ctcttttctg ggaagctgta cctcattgga    840 

attgcagcca ttgtggtagc tgtcattatg atcttcgaga tgattctgag catggtgctg    900 

tgctgtggca tccggaacag ctccgtgtac tgaggccctt cgcttcgcac cagagggtcc    960 

ctggaatgac cagagggcac cttggggggc atggcctgtg tatatactat ttctgtatta   1020 

ctctgctaca cttagccttt ttacttttga gtcttttttg ttctgaactt ttcctgtcac   1080 

cttttggagc cgacaccaca cataggtggc gtatgtgggg acgtagaggt ggagctggcc   1140 

ctagctggca gggccctgta cttctgggac ccctggagag ttctgcctgc tgagccaaac   1200 

ctcctccata gctacttgtc cagaggccac atagccaagc tagaaggcca tgtccaccca   1260 

ctctgcccac tgtgggtcac atcgctccca tctttttaat ccg                     1303 

 
           
             76  
             81  
             PRT  
             Rattus norvegicus  
             
               MOBP-81 protein  
             
           
            76 

Met Ser Gln Lys Val Ala Lys Glu Gly Pro Arg Leu Ser Lys Asn Gln 
1               5                   10                  15 

Lys Phe Ser Glu His Phe Ser Ile His Cys Cys Pro Pro Phe Thr Phe 
            20                  25                  30 

Leu Asn Ser Lys Arg Glu Ile Val Asp Arg Lys Tyr Ser Ile Cys Lys 
        35                  40                  45 

Ser Gly Cys Phe Tyr Gln Lys Lys Glu Glu Asp Trp Ile Cys Cys Ala 
    50                  55                  60 

Cys Gln Lys Thr Arg Leu Arg Arg Arg Ser Arg Ser Thr Pro Arg Lys 
65                  70                  75                  80 

Lys 

 
           
             77  
             2405  
             DNA  
             Rattus norvegicus  
             
               MOBP-81 protein  
             
           
            77 

ctttcacagc agccaatacc tgcagggcaa caaagaatca aatgagagcg agacaagctg     60 

ggaatgaagt cctggttgcc agatgggagc ttgaaaacac agtaagatga gtcaaaaagt    120 

ggccaaggag ggccccaggc tctccaagaa ccagaagttc tcagagcact tcagcatcca    180 

ctgctgccca cccttcacct tcctcaactc caagcgtgag atcgtggacc gcaagtacag    240 

catctgcaag agcggttgct tttaccagaa gaaggaggag gactggatct gctgtgcctg    300 

ccagaagacc agattgagaa ggaggagcag gtcaacccca agaaaaaagt gaccgagaag    360 

ggacttctcg tgaatggaca gcctctgctt gtggatttac tgcttcaccg caacccatct    420 

gccctcagac ttatctggcc tcgagtatga cgcaggggtg gacacctgct gatgtaacaa    480 

ctacagatgc cctcagtccc catggtgtgg gagccttagg gcagcctgcc tggagcactt    540 

ggacgatgcc actccatcac ctccctctat aaccgccgaa gaggcatctc tgtccacctt    600 

ctgaccctcc atggttgttc agtctgaaga ttcctgtgtc ttcagggatg tgcaaaacaa    660 

ggtcatggtg tgcgaccatc caaatggcaa atataaaaaa gaaattgtca agtgttggcg    720 

gtgacgcaag aaaaatccta gaagccacag atggggattt atacactgtc gcaacaccgt    780 

tgagatgtac tctggggaca ctatcaaggc aaagtatctt aaaacctcta atcaagcgac    840 

tgtaatttta ggcatccatc ctggggaaaa ctcacaccct tttagagaaa ggagaaatgc    900 

ccaaatttgt ttaaaagatc gtggtttgta gttaagagag gtagaatgag attaaccaaa    960 

atgtcggtcc acgggagagc aagagagtca atcttggtat gtccacaaag tcctaagagc   1020 

agttaaaagc caatctgtca gacagaccca tgccgagcag gtaaatctag aaagcaaacc   1080 

aaggactata aaagtcaagc accaggcagt aaatccagaa taagagccta tgcatacata   1140 

ttgacagctc acaatcctgt actggttatt gcttacataa ggagaagcca cagatctcat   1200 

tctttcttct agaatgcaat agaatttaaa aatgacagca gaacaaaagg acactagaaa   1260 

ttatcctctc agaaataaaa gtataagaaa taatttctgg cttgaggaaa taacccaggc   1320 

taaaagctac acaggcctgg cttaaccata ccagcagggc atacctttgt taggagaggg   1380 

tcaattcgaa gtttgtgaac agcctgggct gtttccgagg ctgcataaat cttaaacacc   1440 

aaaaatgtgt gttttgaatt aactttgggt ctggtctaca gagtccggct tgtgatggct   1500 

cctgacacgg gagaaaaggg gataagttaa tataaggctc tgggattttc ctttgaggaa   1560 

caggcaggta ggaaaggaca ctagaatgtt ggggaatgtg gagggatgta aaacagacag   1620 

tattatgatg gggggaactc aggaaggaac ggtaaggaca ccagaatggc ctcgtgctgg   1680 

gagccagggt ggccaagccc aggcagatgc acaaagaccc caaaggccac cattgcctca   1740 

caaccagacc cagcaaagtt cagatcaagt gcataaatgg gggtcacgtc agtggaaact   1800 

gtccggagca tggagcaggg ggtgaggaag acagtcagaa ggaagcaaga gatggcttag   1860 

agtgttgcat ggcagcaaga agaagcagtc caaatatcat cccaaggcag ccatggcttc   1920 

ctctcccgag gacactgtgg ccctgtggaa gtgccaaggc agaggcagag gaatttaacc   1980 

gcacacaaac tacatgcaga aactaaggtg ctaaatgaac atctacgtga caacctttct   2040 

gaggaccagg ttccagtgag cggcccaggg ccgttatctt cgcgatggta actgagctga   2100 

gaagaatggg aaccggatag gagcacacag tagcccactg gtgagacaaa tccacgggcg   2160 

agcctcaacc aacccactaa ggaaacttca cgccttttca tctagttttc attttggcaa   2220 

agcaaagcca tcctgagtgc ttgcttgtct ccgccctcca cgccaccccc gagacacaga   2280 

gcatgcgcat taacccagag cgcgctaaca catgcacagc gttggacatt agcctattaa   2340 

tgggaataac cagaaactac taaattttgt ttgttgtaga aataaaacct ggggatggat   2400 

caaaa                                                               2405 

 
           
             78  
             590  
             PRT  
             Homo sapiens  
             
               Syntaxin binding protein 2  
             
           
            78 

Met Ala Pro Ser Gly Leu Lys Ala Val Val Gly Glu Lys Ile Leu Ser 
1               5                   10                  15 

Gly Val Ile Arg Ser Val Lys Lys Asp Gly Glu Trp Lys Val Leu Ile 
            20                  25                  30 

Met Asp His Pro Ser Met Arg Ile Leu Ser Ser Cys Cys Lys Met Ser 
        35                  40                  45 

Asp Ile Leu Ala Glu Gly Ile Thr Ile Val Glu Asp Ile Asn Lys Arg 
    50                  55                  60 

Arg Glu Pro Ile Pro Ser Leu Glu Ala Ile Tyr Leu Leu Ser Pro Thr 
65                  70                  75                  80 

Glu Lys Ala Leu Ile Lys Asp Phe Gln Gly Thr Pro Thr Phe Thr Tyr 
                85                  90                  95 

Lys Ala Ala His Ile Phe Phe Thr Asp Thr Cys Pro Glu Pro Leu Phe 
            100                 105                 110 

Ser Glu Leu Gly Arg Ser Arg Leu Ala Lys Val Val Lys Thr Leu Lys 
        115                 120                 125 

Glu Ile His Leu Ala Phe Leu Pro Tyr Glu Ala Gln Val Phe Ser Leu 
    130                 135                 140 

Asp Ala Pro His Ser Thr Tyr Asn Leu Tyr Cys Pro Phe Arg Ala Glu 
145                 150                 155                 160 

Glu Arg Thr Arg Gln Leu Glu Val Leu Ala Gln Gln Ile Ala Thr Leu 
                165                 170                 175 

Cys Ala Thr Leu Gln Glu Tyr Pro Ala Ile Arg Tyr Arg Lys Gly Pro 
            180                 185                 190 

Glu Asp Thr Ala Gln Leu Ala His Ala Val Leu Ala Lys Leu Asn Ala 
        195                 200                 205 

Phe Lys Ala Asp Thr Pro Ser Leu Gly Glu Gly Pro Glu Lys Thr Arg 
    210                 215                 220 

Ser Gln Leu Leu Ile Met Asp Arg Ala Ala Asp Pro Val Ser Pro Leu 
225                 230                 235                 240 

Leu His Glu Leu Thr Phe Gln Ala Met Ala Tyr Asp Leu Leu Asp Ile 
                245                 250                 255 

Glu Gln Asp Thr Tyr Arg Tyr Glu Thr Thr Gly Leu Ser Glu Ala Arg 
            260                 265                 270 

Glu Lys Ala Val Leu Leu Asp Glu Asp Asp Asp Leu Trp Val Glu Leu 
        275                 280                 285 

Arg His Met His Ile Ala Asp Val Ser Lys Lys Val Thr Glu Leu Leu 
    290                 295                 300 

Arg Thr Phe Cys Glu Ser Lys Arg Leu Thr Thr Asp Lys Ala Asn Ile 
305                 310                 315                 320 

Lys Asp Leu Ser Gln Ile Leu Lys Lys Met Pro Gln Tyr Gln Lys Glu 
                325                 330                 335 

Leu Asn Lys Tyr Ser Thr His Leu His Leu Ala Asp Asp Cys Met Lys 
            340                 345                 350 

His Phe Lys Gly Ser Val Glu Lys Leu Cys Ser Val Glu Gln Asp Leu 
        355                 360                 365 

Ala Met Gly Ser Asp Ala Glu Gly Glu Lys Ile Lys Asp Ser Met Lys 
    370                 375                 380 

Leu Ile Val Pro Val Leu Leu Asp Ala Ala Val Pro Ala Tyr Asp Lys 
385                 390                 395                 400 

Ile Arg Val Leu Leu Leu Tyr Ile Leu Leu Arg Asn Gly Val Ser Glu 
                405                 410                 415 

Glu Asn Leu Ala Lys Leu Ile Gln His Ala Asn Val Gln Ala His Ser 
            420                 425                 430 

Ser Leu Ile Arg Asn Leu Glu Gln Leu Gly Gly Thr Val Thr Asn Pro 
        435                 440                 445 

Gly Gly Ser Gly Thr Ser Ser Arg Leu Glu Pro Arg Glu Arg Met Glu 
    450                 455                 460 

Pro Thr Tyr Gln Leu Ser Arg Trp Thr Pro Val Ile Lys Asp Val Met 
465                 470                 475                 480 

Glu Asp Ala Val Glu Asp Arg Leu Asp Arg Asn Leu Trp Pro Phe Val 
                485                 490                 495 

Ser Asp Pro Ala Pro Thr Ala Ser Ser Gln Ala Ala Val Ser Ala Arg 
            500                 505                 510 

Phe Gly His Trp His Lys Asn Lys Ala Gly Ile Glu Ala Arg Ala Gly 
        515                 520                 525 

Pro Arg Leu Ile Val Tyr Val Met Gly Gly Val Ala Met Ser Glu Met 
    530                 535                 540 

Arg Ala Ala Tyr Glu Val Thr Arg Ala Thr Glu Gly Lys Trp Glu Val 
545                 550                 555                 560 

Leu Ile Gly Ser Ser His Ile Leu Thr Pro Thr Arg Phe Leu Asp Asp 
                565                 570                 575 

Leu Lys Ala Leu Asp Lys Lys Leu Glu Asp Ile Ala Leu Pro 
            580                 585                 590 

 
           
             79  
             1888  
             DNA  
             Homo sapiens  
             
               Syntaxin binding protein 2  
             
           
            79 

ggcacgaggg gcggcggcgc ccctcgggga agatggcgcc ctcggggctg aaggcggtgg     60 

tgggggaaaa aattctgagc ggagttattc ggagtgtcaa gaaggatggg gagtggaagg    120 

tgcttatcat ggatcaccca agcatgcgca tcttgtcttc ctgctgcaaa atgtcagata    180 

tcctggctga gggcatcacc attgttgaag acatcaacaa acggcgggaa cccattccca    240 

gtctggaggc catttatttg ctgagcccca cggagaaggc cctgatcaaa gacttccagg    300 

ggaccccgac tttcacctac aaagcggccc atatcttctt caccgacacc tgccccgagc    360 

ccctgttcag tgagctaggc cgctctcgtc tggcaaaggt ggtgaagacg ttgaaggaga    420 

ttcaccttgc cttcctcccc tacgaggccc aggtgttctc cctcgatgct ccccacagca    480 

cctacaacct ctactgcccc ttccgggcag aggagcgcac gcggcagctc gaggtgctgg    540 

cccagcagat tgccacgctg tgcgccaccc tgcaggagta cccggccatc cgctaccgca    600 

agggcccaga ggacacagcc cagttggccc acgccgtcct ggccaagctg aacgccttca    660 

aggcagacac tcccagtctg ggcgagggcc cagagaaaac ccgctcccag ctgctgataa    720 

tggaccgggc agctgacccc gtgtccccac tactgcatga gctcacgttc caggccatgg    780 

cgtatgatct gctggacata gagcaggaca catacaggta tgagaccacc gggctgagcg    840 

aggcgcggga gaaggccgtc ttgctggacg aggacgatga cttgtgggtg gagcttcgcc    900 

acatgcatat cgcagatgtg tccaagaagg tcacggagct cctgaggacc ttctgtgaga    960 

gcaagaggct gaccacggac aaggcgaaca tcaaagacct atcccagatc ctgaaaaaga   1020 

tgccgcagta ccagaaggag ctgaataagt attctacgca cctgcatcta gcagatgatt   1080 

gtatgaagca cttcaagggc tcggtggaga agctgtgtag tgtggagcag gacctggcca   1140 

tgggctccga cgcagagggg gagaagatca aggactccat gaagctgatc gttccggtgc   1200 

tgctggacgc ggcggtgccc gcctacgaca agatccgggt cctgctgctc tacatcctcc   1260 

ttcggaatgg tgtgagtgag gagaacctgg ccaagctgat ccagcatgcc aatgtacagg   1320 

cgcacagcag cctcatccgt aacctggagc agctgggagg cactgtcacc aaccccgggg   1380 

gctcggggac ctccagccgg ctggagccga gagaacgcat ggagcccacc tatcagctgt   1440 

cccgctggac cccggtcatc aaggatgtaa tggaggacgc cgtggaggac cggctggaca   1500 

ggaacctgtg gcccttcgta tccgaccccg cccccacggc cagctcccag gccgctgtca   1560 

gtgcccgctt cggtcactgg cacaagaaca aggctggcat agaagcccgg gcgggccccc   1620 

ggctcatcgt gtatgtcatg ggcggtgtgg ccatgtcaga gatgagggcc gcctacgagg   1680 

tgaccagggc caccgagggc aagtgggagg tgctcattgg ctcctcacac atcctcaccc   1740 

cgacccgctt cctggatgac ctgaaggcac tggacaagaa gctggaggac attgccctgc   1800 

cctgacccct ggccccgccc cctacccctc cctttccaga gaaataaact cttcccgtcg   1860 

ctctgccaaa aaaaaaaaaa aaaaaaaa                                      1888 

 
           
             80  
             505  
             PRT  
             Rattus norvegicus  
             
               Alpha-internexin  
             
           
            80 

Met Ser Phe Gly Ser Glu His Tyr Leu Cys Ser Ala Ser Ser Tyr Arg 
1               5                   10                  15 

Lys Val Phe Gly Asp Gly Ser Arg Leu Ser Ala Arg Leu Ser Gly Pro 
            20                  25                  30 

Gly Ala Ser Gly Ser Phe Arg Ser Gln Ser Leu Ser Arg Ser Asn Val 
        35                  40                  45 

Ala Ser Thr Ala Ala Cys Ser Ser Ala Ser Ser Leu Gly Leu Gly Leu 
    50                  55                  60 

Ala Tyr Arg Arg Leu Pro Ala Ser Asp Gly Leu Asp Leu Ser Gln Ala 
65                  70                  75                  80 

Ala Ala Arg Thr Asn Glu Tyr Lys Ile Ile Arg Thr Asn Glu Lys Glu 
                85                  90                  95 

Gln Leu Gln Gly Leu Asn Asp Arg Phe Ala Val Phe Ile Glu Lys Val 
            100                 105                 110 

His Gln Leu Glu Thr Gln Asn Arg Ala Leu Glu Ala Glu Leu Ala Ala 
        115                 120                 125 

Leu Arg Gln Arg His Ala Glu Pro Ser Arg Val Gly Glu Leu Phe Gln 
    130                 135                 140 

Arg Glu Leu Arg Glu Leu Arg Ala Gln Leu Glu Glu Ala Ser Ser Ala 
145                 150                 155                 160 

Arg Ala Gln Ala Leu Leu Glu Arg Asp Gly Leu Ala Glu Glu Val Gln 
                165                 170                 175 

Arg Leu Arg Ala Arg Cys Glu Glu Glu Ser Arg Gly Arg Glu Gly Ala 
            180                 185                 190 

Glu Arg Ala Leu Lys Ala Gln Gln Arg Asp Val Asp Gly Ala Thr Leu 
        195                 200                 205 

Ala Arg Leu Asp Leu Glu Lys Lys Val Glu Ser Leu Leu Asp Glu Leu 
    210                 215                 220 

Ala Phe Val Arg Gln Val His Asp Glu Glu Val Ala Glu Leu Leu Ala 
225                 230                 235                 240 

Thr Leu Gln Ala Ser Ser Gln Ala Ala Ala Glu Val Asp Val Ala Val 
                245                 250                 255 

Ala Lys Pro Asp Leu Thr Ser Ala Leu Arg Glu Ile Arg Ala Gln Tyr 
            260                 265                 270 

Glu Ser Leu Ala Ala Lys Asn Leu Gln Ser Ala Glu Glu Trp Tyr Lys 
        275                 280                 285 

Ser Lys Phe Ala Asn Leu Asn Glu Gln Ala Ala Arg Ser Thr Glu Ala 
    290                 295                 300 

Ile Arg Ala Ser Arg Glu Glu Ile His Glu Tyr Arg Arg Gln Leu Gln 
305                 310                 315                 320 

Ala Arg Thr Ile Glu Ile Glu Gly Leu Arg Gly Ala Asn Glu Ser Leu 
                325                 330                 335 

Glu Arg Gln Ile Leu Glu Leu Glu Glu Arg His Ser Ala Glu Val Ala 
            340                 345                 350 

Gly Tyr Gln Asp Ser Ile Gly Gln Leu Glu Ser Asp Leu Arg Asn Thr 
        355                 360                 365 

Lys Ser Glu Met Ala Arg His Leu Arg Glu Tyr Gln Asp Leu Leu Asn 
    370                 375                 380 

Val Lys Met Ala Leu Asp Ile Glu Ile Ala Ala Tyr Arg Lys Leu Leu 
385                 390                 395                 400 

Glu Gly Glu Glu Thr Arg Phe Ser Thr Ser Gly Leu Ser Ile Ser Gly 
                405                 410                 415 

Leu Asn Pro Leu Pro Asn Pro Ser Tyr Leu Leu Pro Pro Arg Ile Leu 
            420                 425                 430 

Ser Ser Thr Thr Ser Lys Val Ser Ser Ala Gly Leu Ser Leu Lys Lys 
        435                 440                 445 

Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Gly Ala Ser Lys Glu Val 
    450                 455                 460 

Thr Lys Lys Thr Ser Lys Val Gly Glu Ser Phe Glu Glu Thr Leu Glu 
465                 470                 475                 480 

Glu Thr Val Val Ser Thr Lys Lys Thr Glu Lys Ser Thr Ile Glu Glu 
                485                 490                 495 

Ile Thr Thr Ser Ser Ser Gln Lys Met 
            500                 505 

 
           
             81  
             4593  
             DNA  
             Rattus norvegicus  
             
               Alpha-internexin  
             
           
            81 

gaattctgaa ctacaaagca aagcgcttca catttccctg acttgtgcca taggctggaa     60 

ggtaaactca gacaactgag ccatccattg ttcaggctct actctgtcct taccgggtgt    120 

ggcgagggtg gccagttctg tcagccgtgc tcctgcttca ctcctggaat tgaatcaaag    180 

actaatcccc acagaaaatg gttctcccat ctaattcaaa atggaatgtc aaacgcatac    240 

agggccagac agatgaaaag acgaggtgga aagggtagct ctggagtgct gggagctgtg    300 

aggaacaccg cacttaaaca aaggcactca cttccgccct ctgcagtcat cctaaacctt    360 

ctactttcaa gagtggcagg ggctgggcat taaaaggtca aaatcaatcc agtctctttt    420 

tagatgaaac ctcctgacta aaaattcatt aaaaaaaaac attttgaaat ctattgtttt    480 

gttggtttgg gtctttttgt ttaaaccaaa gcctgcagac tcctttctac cagcctgcgg    540 

tcctgggatg cgcggttgat tggaggtcta gagatgaacc ctgctaattc tcaacgcttt    600 

gctccttttc caggcctagc ctctttccct ccccgtaacc cttcgcaccc ttggaaataa    660 

gcctcttgtt cttcaccaat gtccggagca tctcacgctc tctgcgaatc ctcccgttta    720 

ctgtggctgc ggagctgaga gatgctctct gaagtggacc cactttctcc gaaaaggacc    780 

aggagacacc taagtccaga agataaagag gagggtgctt ctccccctga aaagggtggg    840 

gacagggcct cacccttggg aactctggga tgcagatgag aacagagaca cctttcagaa    900 

aaacctactc tccttggggg ataagcacag aaaaggaagg acaggctcta aagccatcaa    960 

agagctgagt gctctgggct gggaagccaa ggttgttggg gcactccctc ccctccggtc   1020 

attccctagg cagattcttg gggcacaggc gcagcggcca gggacggccc aggtcggtgg   1080 

cgcgcagctg cggtggcact gcgcttcccc gcccccgcag gccccccttc ctctccccgc   1140 

tagatctgaa gatgaagctc cacccctagc gcgtcgcccc agccccgcgc cttaaaagcc   1200 

ccgcacaccg ccccgccgca cccagccttg ccgcaccttc gcgtcctcgc caggtccgcc   1260 

gcagccgcgc acccggcccc gaccccggca ccatgagctt cggatcagag cactacttgt   1320 

gctccgcctc ctcctaccgc aaggtgttcg gggatggctc gcgcctgtct gcgcgcctgt   1380 

ccgggccggg agcgtccggc agctttcgct cgcagtcgct gtcccgcagc aatgtggcct   1440 

ccacggccgc ttgctcctcg gcctcgtctc tcggcctggg cctggcctac cgccgcctgc   1500 

cggcctccga cgggctggac ctgagccaag cagcggcgcg caccaacgag tacaagatca   1560 

tccgcactaa cgagaaggag cagctgcagg gcctcaacga ccgcttcgcg gtgttcatcg   1620 

aaaaggtgca ccagctggag acgcagaacc gcgcgctcga ggccgagctg gccgcgctgc   1680 

gccagcgcca cgccgagccg tcgcgcgtcg gcgagctctt ccagcgcgag ctgcgcgagc   1740 

tgcgcgcgca gctggaggag gcgagctccg cgcgcgcgca ggccctgctg gagcgcgacg   1800 

ggctggccga ggaggtgcag cgactgcggg cgcgctgcga ggaggagagc cgcgggcgcg   1860 

aaggcgcgga gcgcgccctg aaggcgcagc agcgcgacgt ggacggcgcc actctggccc   1920 

gcctggatct ggagaagaag gtggagtcgc tgctggacga gctggccttc gtgcgccagg   1980 

tgcacgacga ggaggtggcc gagctcctgg ccacgctgca ggcgtcttcg caagccgccg   2040 

ccgaggtgga cgtggctgtg gctaaaccag acctgacttc ggcgctgagg gagatccgcg   2100 

cccagtatga gtccttggcc gctaagaacc tgcagtcggc cgaggagtgg tacaagtcca   2160 

agttcgccaa cctgaacgag caggctgcgc gcagcacgga agccatccga gccagccgag   2220 

aggagatcca cgagtaccgg cgccagctcc aggctcgtac cattgagata gagggcctgc   2280 

gcggagccaa tgaatccctg gagaggcaga tcttggaact ggaggagcgg cacagcgctg   2340 

aggtggccgg ctaccaggat agcattgggc agctggagag tgacctgagg aacaccaaga   2400 

gcgagatggc ccgccacctt cgggaatacc aggacttgct caatgtcaaa atggctcttg   2460 

acattgagat agcagcttac aggaagctgc tggaaggcga agagacacga tttagcacca   2520 

gcgggttaag catctcaggg ctgaatccac ttcccaatcc cagttatttg ctccctccta   2580 

gaatccttag ttctacaacc tccaaagtct catccgctgg gctgtccctg aaaaaggagg   2640 

aggaggagga ggaggaagag gaagaagggg cttctaagga agtcactaag aaaacatcca   2700 

aggtagggga aagtttcgaa gaaacactgg aggaaacggt ggtatctacg aagaaaaccg   2760 

aaaagtcaac tatagaagaa atcaccacca gttccagcca aaaaatgtaa tgctgttgct   2820 

taaaaaaaaa aaacaaaaaa aggcgatgcc taagagggaa tgcacttggc ttattcacag   2880 

cttactcctg aaccacacct accaccgcaa tagaatgtct tcaaggcttc aatgtcatag   2940 

tctgtaccga atcctcgctc tctccagtag gaaggccact gtagctggga gcaaactgca   3000 

gtgctggagg aggagcagga ggagctctct aagatcacag ctttcctacc tacagctcag   3060 

gcctcatagt gacaggtgac ccaggtgcag aggaggctca aacgaaggtg ctaaatctgg   3120 

tcatcgctcg ctgtgagctg aaatcgaaac ctgtttctcc accgtacccc gccagtaccc   3180 

agccatagaa cctcactgta gatgtctaaa aatatacagt cactgccgag gacaagctag   3240 

tgctccagac accattctcc catccccctc tgatagagct gagtcacaaa acgacggtcg   3300 

tctctcatgg gcaggtttcc ttcctctctc ttcaatcccg tattgccatt tctcccacct   3360 

gagaggaagg aacaaagttt agtgtggtat ccccttctag acacgcatag aacttacgcc   3420 

ctttagtcct cactccgtgg gtaaattaga tgcatgttga ccattcccgt gatttgtata   3480 

acgcaccctt gtccatttca tgacctttca tgtccgtagc ttaaaaaata gaaagtgatt   3540 

aatgataatg gcagtgattg gttttaaggg ttttcctaaa ataatactaa ttataataat   3600 

aatcataaaa gggatctcta gactttaggc tttcagctta tccagatcca cttaattaga   3660 

gaggaaaaaa attaccttaa atgatgtagc atttcacctt aaatttagag gcacggtgat   3720 

agttgcccta ctctgcgctt aagctgacct tagacctcgt atcgtaagta gagtgagtga   3780 

gtgagtgctc tgaaagggaa agaacaaggt ctcctctgtg ttggtttcta tgtagtgcta   3840 

tagaattagc tttctcatac ccttgcccta ggatctacat aattcccttc ccattagggt   3900 

agtatgctat gtataggaca ttttatcacc agacacaacg ctactgccca aaagtgaaaa   3960 

acacacactt ctggtcaatc aacaccacca gcaaatattt aaaaaaaata aaaaaggaag   4020 

gaagaaagaa agaaagaaag aaagaaagaa agagagagag agagagaaag agagaaagaa   4080 

agagaaagat tgattctcag agtcttttcc taacagggtt acattgcttc agttggtgga   4140 

tgtccctccc ctgaaggaaa gacagaaaca ttattcaggg tccaggaggg gtatgcacac   4200 

ttctccccca cctcaagaag gcaggctgga aagtcagctc ttcgtgcact caactgtggc   4260 

atccacatga cctgtctgcc tcgcgtgcca aacttgatgc agggtttgcc ctgtctctgc   4320 

tcactcccac caagctgtag aactgtctgt cctgtccccg cccaccccat cgcaagctct   4380 

gtgctgataa ggacatgatg cttctactga attcacattc tagcacgtgc ttcaatatag   4440 

ctcaaggaac cggaaagaca cttcgttttt cctgacccat cccctcttga cccccccccc   4500 

acactgtaag acattagtaa tattaagaaa ttaaaagtca ttaatcctat gtggttcttg   4560 

tctgtggtct ctatagctcc tgaggtgtgc att                                4593 

 
           
             82  
             320  
             PRT  
             Mus musculus  
             
               Beta-sarcoglycan  
             
           
            82 

Met Ala Ala Ala Ala Ala Ala Ala Ala Ala Thr Glu Gln Gln Gly Ser 
1               5                   10                  15 

Asn Gly Pro Val Lys Lys Ser Met Arg Glu Lys Ala Val Glu Arg Arg 
            20                  25                  30 

Asn Val Asn Lys Glu His Asn Ser Asn Phe Lys Ala Gly Tyr Ile Pro 
        35                  40                  45 

Ile Asp Glu Asp Arg Leu His Lys Thr Gly Leu Arg Gly Arg Lys Gly 
    50                  55                  60 

Asn Leu Ala Ile Cys Val Ile Val Leu Leu Phe Ile Leu Ala Val Ile 
65                  70                  75                  80 

Asn Leu Leu Ile Thr Leu Val Ile Trp Ala Val Ile Arg Ile Gly Pro 
                85                  90                  95 

Asn Gly Cys Asp Ser Met Glu Phe His Glu Ser Gly Leu Leu Arg Phe 
            100                 105                 110 

Lys Gln Val Ser Asp Met Gly Val Ile His Pro Leu Tyr Lys Ser Thr 
        115                 120                 125 

Val Gly Gly Arg Arg Asn Glu Asn Leu Val Ile Thr Gly Asn Asn Gln 
    130                 135                 140 

Pro Ile Val Phe Gln Gln Gly Thr Thr Lys Leu Ser Val Glu Lys Asn 
145                 150                 155                 160 

Lys Thr Ser Ile Thr Ser Asp Ile Gly Met Gln Phe Phe Asp Pro Arg 
                165                 170                 175 

Thr His Asn Ile Leu Phe Ser Thr Asp Tyr Glu Thr His Glu Phe His 
            180                 185                 190 

Leu Pro Ser Gly Val Lys Ser Leu Asn Val Gln Lys Ala Ser Thr Glu 
        195                 200                 205 

Arg Ile Thr Ser Asn Ala Thr Ser Asp Leu Asn Ile Lys Val Asp Gly 
    210                 215                 220 

Arg Ala Ile Val Arg Gly Asn Glu Gly Val Phe Ile Met Gly Lys Thr 
225                 230                 235                 240 

Ile Glu Phe His Met Gly Gly Asp Val Glu Leu Lys Ala Glu Asn Ser 
                245                 250                 255 

Ile Ile Leu Asn Gly Thr Val Met Val Ser Pro Thr Arg Leu Pro Ser 
            260                 265                 270 

Ser Ser Ser Gly Asp Gln Ser Gly Ser Gly Asp Trp Val Arg Tyr Lys 
        275                 280                 285 

Leu Cys Met Cys Ala Asp Gly Thr Leu Phe Lys Val Gln Val Thr Gly 
    290                 295                 300 

His Asn Met Gly Cys Gln Val Ser Asp Asn Pro Cys Gly Asn Thr His 
305                 310                 315                 320 

 
           
             83  
             1237  
             DNA  
             Mus musculus  
             
               Beta-sarcoglycan  
             
           
            83 

gagagcgcaa gcgcgggctg cggcgggcca agatggcggc agcggcggcg gcggcggcag     60 

cgaccgagca gcaagggtcc aacgggccag tgaagaaatc catgcgggag aaggctgtgg    120 

agcggaggaa tgttaataaa gagcacaaca gcaatttcaa agctggatat atccccatcg    180 

atgaggaccg gctccataag actgggctga gggggcgaaa gggcaactta gccatctgcg    240 

tgatcgtcct cctgtttatc ctggccgtca tcaatctact cattacactt gtcatctggg    300 

cggtgatccg cattgggcca aatgggtgtg atagcatgga gttccacgag agcggtctgc    360 

tgaggttcaa gcaagtgtct gacatgggcg tcatccaccc gctttataag agcacggtgg    420 

gaggacggcg gaatgaaaac ctggtcatca ctggcaacaa ccagccaatt gtcttccagc    480 

aagggacgac caagctgagt gttgaaaaga acaagacctc catcaccagc gacatcggga    540 

tgcagttttt tgacccaagg acacacaata tcctgttcag cacggactat gagacgcacg    600 

agtttcatct gccaagtggg gtgaaaagtt tgaacgttca gaaagcatcg actgaaagga    660 

ttaccagcaa tgctaccagt gacttaaaca tcaaagtcga tgggcgtgca attgtgcgag    720 

gcaacgaagg cgtcttcatc atgggcaaaa ccattgaatt tcacatggga ggagatgtgg    780 

agttaaaagc tgaaaacagc atcatcctca acggcacggt gatggtcagc cctacgcgcc    840 

tgcccagttc ctccagcggg gaccagtcag ggagtggtga ctgggtgcgc tacaagctct    900 

gcatgtgtgc agatggcaca ctcttcaaag tacaagtaac aggtcacaac atgggctgcc    960 

aggtctcaga caacccttgt gggaacactc attagacggt gcatccgcag gctcagcgcg   1020 

ctcagatctg acttgacctt ccccttttta agaatgcatg tgaaccatgt ttgtacagcg   1080 

tttgccataa cttgtaatta ttttacctgg ggagcaccac tgtgtctatt atgtactctc   1140 

catatttaag ggatcttgag tgcctacatt ctaatacatt ttagtaagtt gcactgatac   1200 

tcaacagttc tctaaaacaa tgatgagaat taataaa                            1237 

 
           
             84  
             251  
             PRT  
             Homo sapiens  
             
               CGI-78 protein  
             
           
            84 

Met Gly Ala Ala Val Phe Phe Gly Cys Thr Phe Val Ala Phe Gly Pro 
1               5                   10                  15 

Ala Phe Ala Leu Phe Leu Ile Thr Val Ala Gly Asp Pro Leu Arg Val 
            20                  25                  30 

Ile Ile Leu Val Ala Gly Ala Phe Phe Trp Leu Val Ser Leu Leu Leu 
        35                  40                  45 

Ala Ser Val Val Trp Phe Ile Leu Val His Val Thr Asp Arg Ser Asp 
    50                  55                  60 

Ala Arg Leu Gln Tyr Gly Leu Leu Ile Phe Gly Ala Ala Val Ser Val 
65                  70                  75                  80 

Leu Leu Gln Glu Val Phe Arg Phe Ala Tyr Tyr Lys Leu Leu Lys Lys 
                85                  90                  95 

Ala Asp Glu Gly Leu Ala Ser Leu Ser Glu Asp Gly Arg Ser Pro Ile 
            100                 105                 110 

Ser Ile Arg Gln Met Ala Tyr Val Ser Gly Leu Ser Phe Gly Ile Ile 
        115                 120                 125 

Ser Gly Val Phe Ser Val Ile Asn Ile Leu Ala Asp Ala Leu Gly Pro 
    130                 135                 140 

Gly Val Val Gly Ile His Gly Asp Ser Pro Tyr Tyr Phe Leu Thr Ser 
145                 150                 155                 160 

Ala Phe Leu Thr Ala Ala Ile Ile Leu Leu His Thr Phe Trp Gly Val 
                165                 170                 175 

Val Phe Phe Asp Ala Cys Glu Arg Arg Arg Tyr Trp Ala Leu Gly Leu 
            180                 185                 190 

Val Val Gly Ser His Leu Leu Thr Ser Gly Leu Thr Phe Leu Asn Pro 
        195                 200                 205 

Trp Tyr Glu Ala Ser Leu Leu Pro Ile Tyr Ala Val Thr Val Ser Met 
    210                 215                 220 

Gly Leu Trp Ala Phe Ile Thr Ala Gly Gly Ser Leu Arg Ser Ile Gln 
225                 230                 235                 240 

Arg Ser Ser Cys Val Arg Thr Asp Tyr Leu Asp 
                245                 250 

 
           
             85  
             1967  
             DNA  
             Homo sapiens  
             
               CGI-78 protein  
             
           
            85 

gtggggtcgc gttgccaccc cacgcggact ccccagctgg cgcgcccctc ccatttgcct     60 

gtcctggtca ggcccccacc ccccttccca cctgaccagc catgggggct gcggtgtttt    120 

tcggctgcac tttcgtcgcg ttcggcccgg ccttcgcgct tttcttgatc actgtggctg    180 

gggacccgct tcgcgttatc atcctggtcg caggggcatt tttctggctg gtctccctgc    240 

tcctggcctc tgtggtctgg ttcatcttgg tccatgtgac cgaccggtca gatgcccggc    300 

tccagtacgg cctcctgatt tttggtgctg ctgtctctgt ccttctacag gaggtgttcc    360 

gctttgccta ctacaagctg cttaagaagg cagatgaggg gttagcatcg ctgagtgagg    420 

acggaagatc acccatctcc atccgccaga tggcctatgt ttctggtctc tccttcggta    480 

tcatcagtgg tgtcttctct gttatcaata ttttggctga tgcacttggg ccaggtgtgg    540 

ttgggatcca tggagactca ccctattact tcctgacttc agcctttctg acagcagcca    600 

ttatcctgct ccataccttt tggggagttg tgttctttga tgcctgtgag aggagacggt    660 

actgggcttt gggcctggtg gttgggagtc acctactgac atcgggactg acattcctga    720 

acccctggta tgaggccagc ctgctgccca tctatgcagt cactgtttcc atggggctct    780 

gggccttcat cacagctgga gggtccctcc gaagtattca gcgcagctct tgtgtaagga    840 

ctgactacct ggactgatcg cctgacagat cccacctgcc tgtccactgc ccatgactga    900 

gcccagcccc agcccggggt ccattgccca cattctctgt ctccttctcg tcggtctacc    960 

ccactacctc cagggttttg ctttgtcctt ttgtgaccgt tagtctctaa gctttaccag   1020 

gagcagcctg ggttcagcca gtcagtgact ggtgggtttg aatctgcact tatccccacc   1080 

acctggggac ccccttgttg tgtccaggac tccccctgtg tcagtgctct gctctcaccc   1140 

tgcccaagac tcacctccct tcccctctgc aggccgacgg caggaggaca gtcgggtgat   1200 

ggtgtattct gccctgcgca tcccacccga ggactgaggg aacctagggg ggacccctgg   1260 

gcctggggtg ccctcctgat gtcctcgccc tgtatttctc catctccagt tctggacagt   1320 

gcaggttgcc aagaaaaggg acctagttta gccattgccc tggagatgaa attaatggag   1380 

gctcaaggat agatgagctc tgagtttctc agtactccct caagactgga catcttggtc   1440 

tttttctcag gcctgagggg gaaccatttt tggtgtgata aataccctaa actgcctttt   1500 

tttctttttt gaggtggggg gagggaggag gtatattgga actcttctaa cctccttggg   1560 

ctatattttc tctcctcgag ttgctcctca tggctgggct catttcggtc cctttctcct   1620 

tggtcccaga ccttggggga aaggaaggaa gtgcatgttt gggaactggc attactggaa   1680 

ctaatggttt taacctcctt aaccaccagc atccctcctc tccccaaggt gaagtggagg   1740 

gtgctgtggt gagctggcca ctccagagct gcagtgccac tggaggagtc agactaccat   1800 

gacatcgtag ggaaggaggg gagatttttt tgtagttttt aattggggtg tgggaggggc   1860 

ggggaggttt tctataaact gtatcatttt ctgctgaggg tggagtgtcc catcctttta   1920 

atcaaggtga ttgtgatttt gactaataaa aaagaatttg taaaaaa                 1967 

 
           
             86  
             885  
             PRT  
             Homo sapiens  
             
               KIAA0143 protein  
             
           
            86 

Ala Gly Gly Gly Ser Cys Arg Pro Leu Gly Cys Val Thr Ala Gly Ser 
1               5                   10                  15 

Ala Ser Ala Pro Ser Thr Leu Arg Pro Ser Pro Phe Ala Ser Ser Arg 
            20                  25                  30 

Pro Pro Arg Pro Ser Asn Gly Arg His Gly Ala Val Gly Ala Pro Cys 
        35                  40                  45 

Ala Ala Pro Leu Ser Leu Gly Ala Ala Ala Ser Ala Val Glu Ile Ala 
    50                  55                  60 

Met Pro Thr Arg Val Cys Cys Cys Cys Ser Ala Leu Arg Pro Arg Tyr 
65                  70                  75                  80 

Lys Arg Leu Val Asp Asn Ile Phe Pro Glu Asp Pro Lys Asp Gly Leu 
                85                  90                  95 

Val Lys Thr Asp Met Glu Lys Leu Thr Phe Tyr Ala Val Ser Ala Pro 
            100                 105                 110 

Glu Lys Leu Asp Arg Ile Gly Ser Tyr Leu Ala Glu Arg Leu Ser Arg 
        115                 120                 125 

Asp Val Val Arg His Arg Ser Gly Tyr Val Leu Ile Ala Met Glu Ala 
    130                 135                 140 

Leu Asp Gln Leu Leu Met Ala Cys His Ser Gln Ser Ile Lys Pro Phe 
145                 150                 155                 160 

Val Glu Ser Phe Leu His Met Val Ala Lys Leu Leu Glu Ser Gly Glu 
                165                 170                 175 

Pro Lys Leu Gln Val Leu Gly Thr Asn Ser Phe Val Lys Phe Ala Asn 
            180                 185                 190 

Ile Glu Glu Asp Thr Pro Ser Tyr His Arg Arg Tyr Asp Phe Phe Val 
        195                 200                 205 

Ser Arg Phe Ser Ala Met Cys His Ser Cys His Ser Asp Pro Glu Ile 
    210                 215                 220 

Arg Thr Glu Ile Arg Ile Ala Gly Ile Arg Gly Ile Gln Gly Val Val 
225                 230                 235                 240 

Arg Lys Thr Val Asn Asp Glu Leu Arg Ala Thr Ile Trp Glu Pro Gln 
                245                 250                 255 

His Met Asp Lys Ile Val Pro Ser Leu Leu Phe Asn Met Gln Lys Ile 
            260                 265                 270 

Glu Glu Val Asp Ser Arg Ile Gly Pro Pro Ser Ser Pro Ser Ala Thr 
        275                 280                 285 

Asp Lys Glu Glu Asn Pro Ala Val Leu Ala Glu Asn Cys Phe Arg Glu 
    290                 295                 300 

Leu Leu Gly Arg Ala Thr Phe Gly Asn Met Asn Asn Ala Val Arg Pro 
305                 310                 315                 320 

Val Phe Ala His Leu Asp His His Lys Leu Trp Asp Pro Asn Glu Phe 
                325                 330                 335 

Ala Val His Cys Phe Lys Ile Ile Met Tyr Ser Ile Gln Ala Gln Tyr 
            340                 345                 350 

Ser His His Val Ile Gln Glu Ile Leu Gly His Leu Asp Ala Arg Lys 
        355                 360                 365 

Lys Asp Ala Pro Arg Val Arg Ala Gly Ile Ile Gln Val Leu Leu Glu 
    370                 375                 380 

Ala Val Ala Ile Ala Ala Lys Gly Ser Ile Gly Pro Thr Val Leu Glu 
385                 390                 395                 400 

Val Phe Asn Thr Leu Leu Lys His Leu Arg Leu Ser Val Glu Phe Glu 
                405                 410                 415 

Ala Asn Asp Leu Gln Gly Gly Ser Val Gly Ser Val Asn Leu Asn Thr 
            420                 425                 430 

Ser Ser Lys Asp Asn Asp Glu Lys Ile Val Gln Asn Ala Ile Ile Gln 
        435                 440                 445 

Thr Ile Gly Phe Phe Gly Ser Asn Leu Pro Asp Tyr Gln Arg Ser Glu 
    450                 455                 460 

Ile Met Met Phe Ile Met Gly Lys Val Pro Val Phe Gly Thr Ser Thr 
465                 470                 475                 480 

His Thr Leu Asp Ile Ser Gln Leu Gly Asp Leu Gly Thr Arg Arg Ile 
                485                 490                 495 

Gln Ile Met Leu Leu Arg Ser Leu Leu Met Val Thr Ser Gly Tyr Lys 
            500                 505                 510 

Ala Lys Thr Ile Val Thr Ala Leu Pro Gly Ser Phe Leu Asp Pro Leu 
        515                 520                 525 

Leu Ser Pro Ser Leu Met Glu Asp Tyr Glu Leu Arg Gln Leu Val Leu 
    530                 535                 540 

Glu Val Met His Asn Leu Met Asp Arg His Asp Asn Arg Ala Lys Leu 
545                 550                 555                 560 

Arg Gly Ile Arg Ile Ile Pro Asp Val Ala Asp Leu Lys Ile Lys Arg 
                565                 570                 575 

Glu Lys Ile Cys Arg Gln Asp Thr Ser Phe Met Lys Lys Asn Gly Gln 
            580                 585                 590 

Gln Leu Tyr Arg His Ile Tyr Leu Gly Cys Lys Glu Glu Asp Asn Val 
        595                 600                 605 

Gln Lys Asn Tyr Glu Leu Leu Tyr Thr Ser Leu Ala Leu Ile Thr Ile 
    610                 615                 620 

Glu Leu Ala Asn Glu Glu Val Val Ile Asp Leu Ile Arg Leu Ala Ile 
625                 630                 635                 640 

Ala Leu Gln Asp Ser Ala Ile Ile Asn Glu Asp Asn Leu Pro Met Phe 
                645                 650                 655 

His Arg Cys Gly Ile Met Ala Leu Val Ala Ala Tyr Leu Asn Phe Val 
            660                 665                 670 

Ser Gln Met Ile Ala Val Pro Ala Phe Cys Gln His Val Ser Lys Val 
        675                 680                 685 

Ile Glu Ile Arg Thr Met Glu Ala Pro Tyr Phe Leu Pro Glu His Ile 
    690                 695                 700 

Phe Arg Asp Lys Cys Met Leu Pro Lys Ser Leu Glu Lys His Glu Lys 
705                 710                 715                 720 

Asp Leu Tyr Phe Leu Thr Asn Lys Ile Ala Glu Ser Leu Gly Gly Ser 
                725                 730                 735 

Gly Tyr Ser Val Glu Arg Leu Ser Val Pro Tyr Val Pro Gln Val Thr 
            740                 745                 750 

Asp Glu Asp Arg Leu Ser Arg Arg Lys Ser Ile Val Asp Thr Val Ser 
        755                 760                 765 

Ile Gln Val Asp Ile Leu Ser Asn Asn Val Pro Ser Asp Asp Val Val 
    770                 775                 780 

Ser Asn Thr Glu Glu Ile Thr Phe Glu Ala Leu Lys Lys Ala Ile Asp 
785                 790                 795                 800 

Thr Ser Gly Met Glu Glu Gln Glu Lys Glu Lys Arg Arg Leu Val Ile 
                805                 810                 815 

Glu Lys Phe Gln Lys Ala Pro Phe Glu Glu Ile Ala Ala Gln Cys Glu 
            820                 825                 830 

Ser Lys Ala Asn Leu Leu His Asp Arg Leu Ala Gln Ile Leu Glu Leu 
        835                 840                 845 

Thr Ile Arg Pro Pro Pro Ser Pro Ser Gly Thr Leu Thr Ile Thr Ser 
    850                 855                 860 

Gly His Ala Gln Tyr Gln Ser Val Pro Val Tyr Glu Met Lys Phe Pro 
865                 870                 875                 880 

Asp Leu Cys Val Tyr 
                885 

 
           
             87  
             5286  
             DNA  
             Homo sapiens  
             
               KIAA0143 protein  
             
           
            87 

ggctggcggc ggtagctgtc gcccgcttgg ttgcgtgacc gcggggtccg cgtccgctcc     60 

ctccaccctt cgcccttcgc ccttcgcctc gtcccggcct ccgcggccca gcaacggccg    120 

tcatggtgcc gtcggcgctc cctgcgcggc cccgctgagc ctcggtgcgg cggcgagcgc    180 

ggtcgagatc gccatgccta cccgagtatg ctgctgctgt tccgctttgc gtcctcgcta    240 

caaacgcctg gtggacaaca tattccctga agatccaaaa gatggccttg tgaaaactga    300 

tatggagaaa ttgacatttt atgcagtatc tgctccagag aaactggatc gaattggttc    360 

ttacctggca gaaaggttga gcagggatgt tgtcagacat cgttctgggt atgttttgat    420 

tgctatggag gcactggacc aacttctcat ggcttgccat tctcaaagca ttaagccatt    480 

tgtagaaagc tttcttcata tggtggcaaa gctgctggaa tcgggggaac caaagcttca    540 

agttcttgga acaaattctt ttgtcaaatt tgcaaatatt gaagaagaca caccatccta    600 

tcacagacgt tatgactttt ttgtgtctcg attcagtgcc atgtgccatt cctgtcatag    660 

tgatccagaa atacgaacag agatacgaat tgctggaatt agaggtattc aaggtgtggt    720 

tcgcaaaaca gtcaacgatg aacttcgggc caccatttgg gaacctcagc atatggataa    780 

gattgttcca tccctcctgt ttaacatgca aaagatagaa gaagttgaca gtcgcatagg    840 

ccctccttct tctccttctg caactgacaa agaagagaat cctgctgtgc tggctgaaaa    900 

ctgtttcaga gaactgctgg gtcgagcaac ttttgggaat atgaataatg ctgttagacc    960 

agtttttgcg catttagatc atcacaaact gtgggatccc aatgaatttg cagttcactg   1020 

ctttaaaatt ataatgtatt ccattcaggc tcagtattct caccatgtga tccaggagat   1080 

tctaggacac cttgatgctc gtaaaaaaga tgctccccgg gttcgagcag gtattattca   1140 

ggttctgtta gaggctgttg ccattgctgc taaaggttcc ataggtccga cagtgctgga   1200 

agtcttcaat acccttttga aacatctgcg tctcagcgtt gaattcgaag caaatgattt   1260 

acagggggga tctgtaggca gtgtcaactt aaatacaagt tccaaagaca atgatgagaa   1320 

gattgtgcag aatgctatca tccaaacaat aggatttttt ggaagtaacc taccagatta   1380 

tcagaggtca gaaatcatga tgttcattat ggggaaagta cctgtctttg gaacatctac   1440 

ccatactttg gatatcagtc aactagggga tttgggaacc aggagaattc agataatgtt   1500 

gctgagatct ttgcttatgg tgacctctgg atataaagcg aagacgattg ttactgcact   1560 

gccagggtct ttcctggatc ctttgttatc accatctctc atggaggact acgaactgag   1620 

acagttggtc ttggaagtaa tgcataatct catggatcgt catgacaata gggcaaagct   1680 

tcgagggatc agaataatac cggatgtagc tgacctaaag ataaaaagag aaaaaatttg   1740 

cagacaagac acaagtttca tgaaaaagaa tgggcaacag ctgtatcggc acatatattt   1800 

gggttgtaaa gaggaagaca acgttcagaa aaactatgaa ctactttata cttctcttgc   1860 

tcttataact attgaactgg ctaatgaaga agtagttatt gatctcattc gactggccat   1920 

tgctttacag gacagtgcaa ttatcaatga ggataatttg ccaatgttcc atcgttgtgg   1980 

aatcatggca ctggttgcag catacctcaa ctttgtaagt cagatgatag ctgtccctgc   2040 

attttgccag catgttagca aggttattga aattcgaact atggaagccc cttattttct   2100 

accagagcat atcttcagag ataagtgcat gcttccaaaa tctttagaga agcatgaaaa   2160 

agatttgtac tttctgacca acaagattgc agagtcgcta ggtggaagtg gatatagtgt   2220 

tgagagattg tcagttccgt atgtaccaca agtaacagat gaagatcgac tttctagaag   2280 

aaaaagcatt gtggacaccg tatccattca ggtggatatt ttatccaaca atgttccttc   2340 

tgatgatgtg gttagtaaca ctgaagaaat cacttttgaa gcattgaaga aagcaattga   2400 

taccagtgga atggaagaac aggaaaagga aaagaggcgt cttgtgatag agaaatttca   2460 

gaaagcacct tttgaagaaa tagcagcaca gtgtgaatcc aaagcaaatt tgcttcatga   2520 

tagacttgcc caaatattgg aactcaccat acgtcctcct cccagtccat caggaacact   2580 

gaccattact tctgggcatg cccaatacca atctgtccca gtctatgaga tgaagtttcc   2640 

agatctgtgt gtgtactgat cggcgcatga agacctcagc atatgatttg taaagcctaa   2700 

aaattaaggc caagctgagc tttcagggtt tacttaatgt gtattaacat acttcttgaa   2760 

aataatgatg gaacatatct ttaaccaaat gtttggcata ccatattaga agttttggag   2820 

ctatatataa tttcgagtac tttcaaagat agatttatgc catgttaatt tgctttgagg   2880 

ttcctgttgc ctttttaagt tgaacatgtt ttggtttcac tttattccac tgttaagtag   2940 

tatgttttaa acttttcaca aatgtaatgt tttttaaaaa gtaagccttc agaggattga   3000 

aactgtataa attgtttatc tcttaaacat ctacacagcc gcttagatgt agaatttttg   3060 

ttgttgtttt ctgcaaaggc agatacattt aaatatattc ctagtcctgg ggctgcaaaa   3120 

ctgttcggtg gctttttgtc cccatgcttt agataagctg ggataggcac cttgctattc   3180 

agttactata ataatatgtg ataggcattc ctcatcttct tcacaataat aggacatctg   3240 

ttgaatagca ttcctcgaat ataaccctaa aaacgccata ctttaaattg tctggttctt   3300 

gtaatattgt gtttcctgcc aagagtttga ccattctgct tgagaagtgt agagcttact   3360 

cttggagtac caaactgtgc aatattttta catcataaga tgtattagtt tacaggctgt   3420 

gctttgaaat tatagtagta ttttgctgtg gctccattaa ttaaatgaga tatatattta   3480 

gtgcagaaaa aagacattta aaacagctat tagttcacct gtgaagagtc tgtacatttt   3540 

gatttctatt aagagcacat ttatttacat ttgtatatta ttttaaatcc tcatagtcaa   3600 

aaaattctcc agatggactt ttaatttgta agatttgaac tgtgacttgt atacatctgt   3660 

ttagaatcaa ttatatttga aaagctgcct gtgttttaac agtcaagtgt gctaaagttt   3720 

gtcaatttaa gctgcttttg atttcagcta ccaagattac aggtgcactc tacacataac   3780 

actgacagac ccataacatt acatacatct tagtgaattc tatcacatgg taaaatgaac   3840 

agctttcttt gtaactcata aaattctctt aggacatttt tataaagtca cctgtttata   3900 

gttctatctt ttcagattcc atttcttttt acataaaaca gcatacatat caaaaactgt   3960 

agcctagaat acagtttaat ttttggcttt tgtttttgtt taaaaaattg cagtgaagaa   4020 

tgggatgttt gtgtttatgg ctatttgggc acctttagta gaaacagaca aaagtaagga   4080 

aacgataata ggacaagcat acttgaaaat ttctgaatac ttaaacaaaa gcgcaacatc   4140 

ttgaaaacca gtctagtcat tgaaacctat gaaatgacac tgaaagatcc tgggctgctt   4200 

cttaaataag tagatcagca agacttgttt cagagtgaca gtggagtcgt taccgctgga   4260 

ggactaaagg gccctgtggc agctgtcact ggaactttgc ctcttgatca ggaaaaatgc   4320 

ttcaccagtc cgtaaagcca agttgtattt tttttattgc ccttttttcc ttctgtattt   4380 

ttaaagaagg atgttaattt ttgactatat attttaaaaa aatctaagca gggggacatg   4440 

caaaaacaat catcatccac ttggatgtca ttttatagat taacactgtg tgcttttgta   4500 

tggaaaaaat atatataatt taatagtata aaaaataaaa tatatattca tttgcactta   4560 

cgtgaaacac aaactttgct ctacaaaatt tcgtgtttct tagtgatttt aaaatgcatg   4620 

tattgcatgt aaaggaaaac cattacaatt aatgtttatc acacctttat cttggtcttt   4680 

gttgatttgg gttttgttgg ggtttttgtt actgttttta aattacagta ggcttctata   4740 

tatcctggat ttctgaactg gtcttgttga caaggattcc caagaaatgg atcttttcac   4800 

tggctgactc tcccatatct gcaagagatt ctgcaggaac tgggtgtgca cacggtgttg   4860 

tagccagttc aggtactgaa tatttaggat ttggtgaagt tcactgtatt gctatatttt   4920 

tgtagataaa taaaactcaa taaattgtta atcattctct ttttgctgta tagaattgct   4980 

tatatcactc tttctttcat gacattggtt aacatttaaa tgttcctcct gtacttgtgt   5040 

tgtctgtgac cgcttataga gttttattgt tattggtgtt tacctgaata cctatgcgta   5100 

cacacacaca tatttcctta atacttctga actcattatc ttttagaata ataatactac   5160 

tacactttac cagcaattaa cttctcccta cccaaaatgt ttttcttcct gtctgaaaat   5220 

ggaactaatt tgtcttattc gtgcttatat ctgtattaaa tgcaataaag ttagtttttg   5280 

aaatgt                                                              5286 

 
           
             88  
             424  
             PRT  
             Homo sapiens  
             
               Septin 2  
             
           
            88 

Thr Asp Ile Ala Arg Gln Val Gly Glu Gly Cys Arg Thr Val Pro Leu 
1               5                   10                  15 

Ala Gly His Val Gly Phe Asp Ser Leu Pro Asp Gln Leu Val Asn Lys 
            20                  25                  30 

Ser Val Ser Gln Gly Phe Cys Phe Asn Ile Leu Cys Val Gly Glu Thr 
        35                  40                  45 

Gly Leu Gly Lys Ser Thr Leu Met Asp Thr Leu Phe Asn Thr Lys Phe 
    50                  55                  60 

Glu Gly Glu Pro Ala Thr His Thr Gln Pro Gly Val Gln Leu Gln Ser 
65                  70                  75                  80 

Asn Thr Tyr Asp Leu Gln Glu Ser Asn Val Arg Leu Lys Leu Thr Ile 
                85                  90                  95 

Val Ser Thr Val Gly Phe Gly Asp Gln Ile Asn Lys Glu Asp Ser Tyr 
            100                 105                 110 

Lys Pro Ile Val Glu Phe Ile Asp Ala Gln Phe Glu Ala Tyr Leu Gln 
        115                 120                 125 

Glu Glu Leu Lys Ile Arg Arg Val Leu His Thr Tyr His Asp Ser Arg 
    130                 135                 140 

Ile His Val Cys Leu Tyr Phe Ile Ala Pro Thr Gly His Ser Leu Lys 
145                 150                 155                 160 

Ser Leu Asp Leu Val Thr Met Lys Lys Leu Asp Ser Lys Val Asn Ile 
                165                 170                 175 

Ile Pro Ile Ile Ala Lys Ala Asp Ala Ile Ser Lys Ser Glu Leu Thr 
            180                 185                 190 

Lys Phe Lys Ile Lys Ile Thr Ser Glu Leu Val Ser Asn Gly Val Gln 
        195                 200                 205 

Ile Tyr Gln Phe Pro Thr Asp Asp Glu Ser Val Ala Glu Ile Asn Gly 
    210                 215                 220 

Thr Met Asn Ala His Leu Pro Phe Ala Val Ile Gly Ser Thr Glu Glu 
225                 230                 235                 240 

Leu Lys Ile Gly Asn Lys Met Met Arg Ala Arg Gln Tyr Pro Trp Gly 
                245                 250                 255 

Thr Val Gln Val Glu Asn Glu Ala His Cys Asp Phe Val Lys Leu Arg 
            260                 265                 270 

Glu Met Leu Ile Arg Val Asn Met Glu Asp Leu Arg Glu Gln Thr His 
        275                 280                 285 

Thr Arg His Tyr Glu Leu Tyr Arg Arg Cys Lys Leu Glu Glu Met Gly 
    290                 295                 300 

Phe Lys Asp Thr Asp Pro Asp Ser Lys Pro Phe Ser Leu Gln Glu Thr 
305                 310                 315                 320 

Tyr Glu Ala Lys Arg Asn Glu Phe Leu Gly Glu Leu Gln Lys Lys Glu 
                325                 330                 335 

Glu Glu Met Arg Gln Met Phe Val Gln Arg Val Lys Glu Lys Glu Ala 
            340                 345                 350 

Glu Leu Lys Glu Ala Glu Lys Glu Leu His Glu Lys Phe Asp Arg Leu 
        355                 360                 365 

Lys Lys Leu His Gln Asp Glu Lys Lys Lys Leu Glu Asp Lys Lys Lys 
    370                 375                 380 

Ser Leu Asp Asp Glu Val Asn Ala Phe Lys Gln Arg Lys Thr Ala Ala 
385                 390                 395                 400 

Glu Leu Leu Gln Ser Gln Gly Ser Gln Ala Gly Gly Ser Gln Thr Leu 
                405                 410                 415 

Lys Arg Asp Lys Glu Lys Lys Asn 
            420 

 
           
             89  
             4612  
             DNA  
             Homo sapiens  
             
               Septin 2  
             
           
            89 

cgaccgatat agctcgccag gtgggtgaag gttgccgaac tgtccccctg gctggacatg     60 

tggggtttga cagcttgcct gaccagctgg tgaataagtc cgtcagccag ggcttctgct    120 

tcaacatcct gtgcgtggga gagacaggtt tgggcaagtc caccctcatg gacaccctgt    180 

tcaacaccaa attcgaaggg gagccagcca cccacacaca gccgggtgtc cagctccagt    240 

ctaataccta tgacctccaa gagagcaacg tgaggctaaa gctcacgatc gttagcacag    300 

ttggctttgg ggaccagatc aacaaagagg acagctacaa gcctatcgtg gaattcatcg    360 

atgcacaatt cgaggcctac ctgcaggaag agctaaagat ccgaagagtg ctacacacct    420 

accatgactc ccgaatccat gtctgcttgt atttcattgc ccccacgggt cattccctga    480 

agtctctgga cctagtgact atgaagaagc tggacagtaa ggtgaacatc atccccatca    540 

ttgccaaagc agatgccatt tcgaagagtg agctaacaaa gttcaaaatc aaaatcacca    600 

gcgagcttgt cagcaacgga gtccagatct atcagtttcc tacagatgat gagtcggtgg    660 

cagagatcaa tggaaccatg aacgcccacc tgccgtttgc tgtcattggc agcacagaag    720 

aactgaagat aggcaacaag atgatgaggg cgcggcagta tccttggggc actgtgcagg    780 

ttgaaaacga ggcccactgc gactttgtga agctgcggga gatgctgatt cgggtcaaca    840 

tggaggatct gcgggagcag acccacaccc ggcactatga gctgtatcgc cgctgtaagc    900 

tggaggagat gggcttcaag gacaccgacc ctgacagcaa acccttcagt ttacaggaga    960 

catatgaggc caaaaggaac gagttcctag gggaactcca gaaaaaagaa gaggagatga   1020 

gacagatgtt cgtccagcga gtcaaagaga aagaagcgga gctcaaagag gcagagaaag   1080 

agctgcacga gaagtttgac cgtctgaaga aactgcacca ggacgagaag aagaaactgg   1140 

aggataagaa gaaatccctg gatgatgaag tgaatgcttt caagcaaaga aagacggcgg   1200 

ctgagctgct ccagtcccag ggctcccagg ctggaggctc acagactctg aagagagaca   1260 

aagagaagaa aaattaactc tgctgtttgc tgcatgctgc atgagaccca gggtcctgtt   1320 

tgggcttcct gtagacaccc ttttcctgcg caacagagct gggcctccct ttctctaatt   1380 

tcccccttaa catgcctggg gggcatacaa tccaacccgc gccctctcct ctcttcctgc   1440 

caaggtttat agaaacctga gaatctgagg gtgatgtctg gccgctggtc aagaagccaa   1500 

cagtcatgtg gctcgcagat gcatcctgca tcccagtccc cctcccagca cccccagcca   1560 

tcccccctgt cttcccccac atctttgcca gaggtgtgac atggtcaggg ggcccatctg   1620 

ctactctttc ccaccagctc ccctgttcca gttctggttg ctgttagttt ccctgaggta   1680 

tttgcaacca ccatggctgg gtaaccaccg atcagcacag ctgtcccctt ggtctcctgt   1740 

atcccagtca ctagtcctcc ctggtccacc ccaccctcat cctcaggagc cacagccatt   1800 

tcttagaggg tttcaaaagg acagcctttg gcgccttttc cttctaacct ttgagtccag   1860 

ccctttccag ttttcattca ctcgaagtaa ctgcactcaa gctgtgctca aaatcggcaa   1920 

cgcatttatt tacaccaagc ccttcccata aaacacaact gctgaagaaa atagcagacg   1980 

tttcccctct ctctaactct gggtatccca cagatgcaaa agggagaata aacctgaata   2040 

ttattaccag cctagagtct tgaatgatag ccttaccgaa ttcttcttgt gaggtatttc   2100 

agcatctcgg ggggtaattt ccggaagggc tccatactgt cccaataagg tgaggccagt   2160 

agcaggaata ataaatccca ctttgtaggc tggaaaactg agctgtcaaa agaatcaagt   2220 

gtttgggggt ttgctctgat gagtcttcta gttcatttgg tgaatgtcat gatgattttt   2280 

aacatgcatt ttgcatgcat cccccaataa gaagagatga gactcggctg gagagaagaa   2340 

aaggccctta actttctttc caatttaagg agttgagagt ttaaaaatat tccagcccta   2400 

agtttttatc atgggtccca tctgatagtg gctttgggaa cctctgtgaa gtagagagcc   2460 

ctcccttgtc agggttatga ggcacagtgg cctttggtgt ttggccagtg acagtgtgag   2520 

agatggagtt gacctggcaa tgatctgtgg ctaacatgcc gtctctctgc ccttcctttg   2580 

cagtaatcca tggctgtgta ctgaatagta ttccccgcta cagctggact ggactccatt   2640 

tagcctttta agccgaggtt cctattttaa ctgacagctt tcctttgggg tgccaggcag   2700 

cgaggccccc cacccctatc ctgccatgta cttcaagctc acttcttctt tttgagttcc   2760 

gcaacttgct cctgcctccc agccccactg gcactgacca tgaccaccta cttctatttt   2820 

ttttttagag tttctttttt tgatcactta ctttcaaagc acacagtcaa acaaggttat   2880 

gccaaatttc caggcctttt tgaagtattg agaaggggaa ggggatttct cacttcaatt   2940 

atagatcata ataggaagca aaaagaaaaa aatgaaaagc aaacatatgc acgcactttt   3000 

cttgttgaca aagcaagaat ataggtttgc tgtgtaggtt tggtgctcta ttgattggtg   3060 

agtgaccaga gcaagtatga aggtgatgct gccaaagcac aagccttttt gaagtattga   3120 

gaaggggaag gggatttctc acttcaatta tagatcataa taggaagcaa aaagaaaaaa   3180 

atgaaaagca aacatatgca cgcacttttc ttgttgacaa agcaagaata taggtttgct   3240 

gtgtaggttt ggtgctctat tgattggtga gtgaccagag caagtatgaa ggtgatgctg   3300 

ccaaagcaca agccagtttc ttgggaaaat tcaagttaca gtggagtatt tttttgaaga   3360 

ccatatgctt ggaggtagaa acaaaccaac gaccaaaaaa aaaaaaaaaa aatctgctca   3420 

gatactcagc cagtagctca gagagatgct gagttaggcc tgtcaggtct ccttgggaaa   3480 

ggcttcatat ttgcaacttt gatgattcta tgtccagctt cagagctgct ttcccagaaa   3540 

ttcacgctta aacaaccaac cggtaaccac cacttcccca caccgccgcc cggtaattat   3600 

ttgcattaca aaccggaggc gccctcattt gcatttgtgt acagattaac tagttaaggc   3660 

ttgagaagct ctgaataatt caaaagtatt agacccacac agccttggag agaccttcag   3720 

aaactaagga ggagttttat attaagggag acattttagt cagtaagacg atataaccta   3780 

cttactccgt aaggggaaat gaaggcccag agaagggaag ggacttgacc gaggtcccac   3840 

ttctgtttcg aggcagaagc cagactaatt ttcatgcctc ctgactccca atcagtttca   3900 

caaagggatt caatctgttt atatacgtta cattcctgga tacgaggtct tttgatgttc   3960 

agagtaactg actagttagt attagaagac cctcgaggtt tttttccaca gaaaaacatc   4020 

tgaagatgga ttgggtgagg gctggcaaaa cgaaggcatg ccgggccagc tccttaaccc   4080 

aatgacccag tgatgctgca aggctggaac ggggtccagg agactgtgtg taacaggtgc   4140 

cctaggtgac ccttataatc agggaagttt ggtgaacaaa aatcgaaccc atgagtgaac   4200 

ataaattaaa aagttgatca acctattaaa atgtgtattt cattgggtag cttttctcac   4260 

tgtagacaga ttttttcctt cttcaatgaa aaggctttta aattagtaca actgttacta   4320 

tttaaaaaaa aaatacccta agtactctgt ttacttctgg tgaaacaaaa ccagtcatta   4380 

gaaatggtct gtgcttttat tttcccagac tggagtggct tttctgaaac acacacacac   4440 

acacacacac acacacacac gtacacacat ccctcacttc tcttaagcca agaagtttgc   4500 

tttccctagc tgcagtgtag atggctcttg tttttgtttt tttgttttaa tcatttggca   4560 

ttcacatgtg gctgttaata tgtgcttgtt tttaattaaa acaagaagct tt           4612 

 
           
             90  
             459  
             PRT  
             Rattus norvegicus  
             
               Nucleobindin  
             
           
            90 

Met Pro Thr Ser Val Pro Arg Gly Ala Pro Phe Leu Leu Leu Pro Pro 
1               5                   10                  15 

Leu Leu Met Leu Ser Ala Val Leu Ala Val Pro Val Asp Arg Ala Ala 
            20                  25                  30 

Pro His Gln Glu Asp Asn Gln Ala Thr Glu Thr Pro Asp Thr Gly Leu 
        35                  40                  45 

Tyr Tyr His Arg Tyr Leu Gln Glu Val Ile Asn Val Leu Glu Thr Asp 
    50                  55                  60 

Gly His Phe Arg Glu Lys Leu Gln Ala Ala Asn Ala Glu Asp Ile Lys 
65                  70                  75                  80 

Ser Gly Lys Leu Ser Gln Glu Leu Asp Phe Val Ser His Asn Val Arg 
                85                  90                  95 

Thr Lys Leu Asp Glu Leu Lys Arg Gln Glu Val Ser Arg Leu Arg Met 
            100                 105                 110 

Leu Leu Lys Ala Lys Met Asp Ala Lys Gln Glu Pro Asn Leu Gln Val 
        115                 120                 125 

Asp His Met Asn Leu Leu Lys Gln Phe Glu His Leu Asp Pro Gln Asn 
    130                 135                 140 

Gln His Thr Phe Glu Ala Arg Asp Leu Glu Leu Leu Ile Gln Thr Ala 
145                 150                 155                 160 

Thr Arg Asp Leu Ala Gln Tyr Asp Ala Ala His His Glu Glu Phe Lys 
                165                 170                 175 

Arg Tyr Glu Met Leu Lys Glu His Glu Arg Arg Arg Tyr Leu Glu Ser 
            180                 185                 190 

Leu Gly Glu Glu Gln Arg Lys Glu Ala Glu Arg Lys Leu Gln Glu Gln 
        195                 200                 205 

Gln Arg Arg His Arg Glu His Pro Lys Val Asn Val Pro Gly Ser Gln 
    210                 215                 220 

Ala Gln Leu Lys Glu Val Trp Glu Glu Leu Asp Gly Leu Asp Pro Asn 
225                 230                 235                 240 

Arg Phe Asn Pro Lys Thr Phe Phe Ile Leu His Asp Ile Asn Ser Asp 
                245                 250                 255 

Gly Val Leu Asp Glu Gln Glu Leu Glu Ala Leu Phe Thr Lys Glu Leu 
            260                 265                 270 

Glu Lys Val Tyr Asp Pro Lys Asn Glu Glu Asp Asp Met Arg Glu Met 
        275                 280                 285 

Glu Glu Glu Arg Leu Arg Met Arg Glu His Val Met Lys Asn Val Asp 
    290                 295                 300 

Thr Asn Gln Asp Arg Leu Val Thr Leu Glu Glu Phe Leu Ala Ser Thr 
305                 310                 315                 320 

Gln Arg Lys Glu Phe Gly Glu Thr Ala Glu Gly Trp Lys Thr Val Glu 
                325                 330                 335 

Met Tyr Pro Ala Tyr Thr Glu Glu Glu Leu Lys Arg Phe Glu Glu Glu 
            340                 345                 350 

Leu Ala Ala Arg Glu Ala Glu Leu Asn Ala Arg Ala Gln Arg Leu Ser 
        355                 360                 365 

Gln Glu Thr Glu Ala Leu Gly Arg Ser Gln Asp Arg Leu Glu Ala Gln 
    370                 375                 380 

Lys Arg Glu Leu Gln Gln Ala Val Leu Gln Met Glu Gln Arg Lys Gln 
385                 390                 395                 400 

Gln Gln Gln Glu Gln Ser Ala Pro Pro Ser Gln Pro Asp Gly Gln Leu 
                405                 410                 415 

Gln Phe Arg Ala Asp Thr Gly Asp Ala Pro Val Pro Ala Pro Ala Gly 
            420                 425                 430 

Asp Gln Lys Asp Val Pro Ala Ser Glu Lys Lys Val Pro Glu Gln Pro 
        435                 440                 445 

Pro Val Leu Pro Gln Leu Asp Ser Gln His Leu 
    450                 455 

 
           
             91  
             2152  
             DNA  
             Rattus norvegicus  
             
               Nucleobindin  
             
           
            91 

ccagaaacct ggcggaggtc gcgggctggg aaaacgccct ctgcgctagg agagaccagc     60 

tacgatgcct acctctgtgc cccgcggggc gcctttcctt ctcctaccac ctttactgat    120 

gctgtctgct gtgctggcag tgcctgtgga ccgcgcagca cctcatcagg aggacaacca    180 

ggccactgag accccggaca caggcctgta ctaccatcgg tacctccagg aggtcatcaa    240 

cgtgctagag acagatgggc acttccggga gaagctgcaa gctgccaacg ctgaggacat    300 

taagagtgga aagctgagtc aagagctgga cttcgtcagc cacaacgtcc gcaccaagct    360 

ggatgagctc aagcgacagg aggtatcaag gcttcggatg ctgctcaagg ccaagatgga    420 

tgcaaagcag gagcctaact tgcaggtgga ccacatgaac cttctgaagc agtttgaaca    480 

cctggaccct cagaaccagc acacgtttga ggctcgggac ctagagctgc tgatccagac    540 

ggccacccga gacctcgccc agtatgacgc tgcacaccat gaagagttca aacgctacga    600 

gatgctcaag gaacatgagc gaagacgtta cctggagtct ctgggagagg agcagcggaa    660 

ggaggctgag aggaagctac aagagcagca gcgcagacac cgggaacacc ccaaagtcaa    720 

tgttcctggc agccaagccc agttgaagga ggtgtgggag gagctagatg gattggaccc    780 

caacaggttc aaccccaaga ccttcttcat actgcatgac atcaacagcg atggtgtcct    840 

agatgagcaa gaactggaag ctctgtttac caaggagctg gagaaggtgt acgacccgaa    900 

gaacgaggag gacgacatga gggagatgga ggaggagcgg ctgcgcatgc gggagcatgt    960 

gatgaagaac gtggacacca accaggaccg acttgtgacc ctggaggaat tcctggcatc   1020 

cacacagagg aaggagtttg gggaaactgc ggagggatgg aagacagtgg aaatgtatcc   1080 

agcctacaca gaggaagagc tgaagcgttt tgaagaggag cttgctgccc gggaggctga   1140 

gctaaatgcc agagcccagc gcctcagcca ggagacagag gccctggggc gctcccagga   1200 

ccgcctagag gctcagaaga gagagctgca gcaggccgtt ctgcagatgg agcaaaggaa   1260 

gcagcaacag caagagcaga gcgctccgcc ttcccaacct gatgggcagc tgcagttccg   1320 

tgcagacaca ggggatgctc ctgtcccagc tccagcaggt gaccagaaag acgtgcctgc   1380 

ttctgaaaag aaggttccag agcagccccc tgtgctgcca cagctggatt ctcagcattt   1440 

ataagccctc agagatcccg gccctcaagt tcccacagac agtgatggaa gctggatgaa   1500 

gtgtccttgc agcctttctg gagaccaaag ccttcatggc ctccagggga gaacagggcc   1560 

accctgttcc tcagatgcgc ttctgcccat gtctaccagt ctgttctcac agcctctggg   1620 

tcccagtcct gccttcctcc ctcccttcct tggcagggac tcaactgcag gagcccctca   1680 

ccccacctga gtgcacaccc caggcctgcc tgccttccat gctttgctct tacctgtccc   1740 

aggctcaacc tttctgcttc ctgtacctgt cagtgggtca caggagcttg acttggtctc   1800 

tccaactctt tgccttgtga gaggccaatg atctcaattc cttttggtgg ctagaggccg   1860 

ccacaatgtg gggtgtccta tcagctctgc ctgggactcc tgaactccac caccacccca   1920 

ccagccttac cctaaccacc tgggcccaat acctacctga gggtcccaga aggggcccag   1980 

atatggctac accccttact gaggtccagc ctcactccca cgtatggggg ctcctgaaaa   2040 

ttggttcacc cacttccctg gcttcaggcc acactgctgt gtcctgctct gtgtcccagt   2100 

ctcatccaac tatagcctgg cacaaataaa cattagatat ctgtgtgaat gt           2152 

 
           
             92  
             160  
             PRT  
             Rattus norvegicus  
             
               Myelin structural protein SR 13  
             
           
            92 

Met Leu Leu Leu Leu Leu Gly Ile Leu Phe Leu His Ile Ala Val Leu 
1               5                   10                  15 

Val Leu Leu Phe Val Ser Thr Ile Val Ser Gln Trp Leu Val Gly Asn 
            20                  25                  30 

Gly His Arg Thr Asp Leu Trp Gln Asn Cys Thr Thr Ser Ala Leu Gly 
        35                  40                  45 

Ala Val Gln His Cys Tyr Ser Ser Ser Val Ser Glu Trp Leu Gln Ser 
    50                  55                  60 

Val Gln Ala Thr Met Ile Leu Ser Val Ile Phe Ser Val Leu Ser Leu 
65                  70                  75                  80 

Phe Leu Phe Phe Cys Gln Leu Phe Thr Leu Thr Lys Gly Gly Arg Phe 
                85                  90                  95 

Tyr Ile Thr Gly Val Phe Gln Ile Leu Ala Gly Leu Cys Val Met Ser 
            100                 105                 110 

Ala Ala Ala Ile Tyr Thr Val Arg His Ser Glu Trp His Val Asn Asn 
        115                 120                 125 

Asp Tyr Ser Tyr Gly Phe Ala Tyr Ile Leu Ala Trp Val Ala Phe Pro 
    130                 135                 140 

Leu Ala Leu Leu Ser Gly Ile Ile Tyr Val Ile Leu Arg Lys Arg Glu 
145                 150                 155                 160 

 
           
             93  
             1736  
             DNA  
             Rattus norvegicus  
             
               Myelin structural protein SR 13  
             
           
            93 

gggggaagcc agcaacctag aggacgcccc cgagtttgtg cctgaggcta ctccgctctg     60 

agccggctgt ccctttgaac tgaaagcacc gctccaccga gcccgagccc aactccagcc    120 

accatgcttc tactcttgtt ggggatcctg ttccttcaca tcgcggtgct agtgttgctc    180 

ttcgtctcca ccatcgtcag ccaatggctc gtgggcaatg gacacaggac tgatctctgg    240 

cagaactgta ccacatccgc cttgggagcc gtccagcact gctactcctc atctgtgagc    300 

gaatggcttc agtctgtcca ggccaccatg atcctgtctg tcatcttcag cgtcctgtcc    360 

ctgttcctgt tcttctgcca gctcttcact ctcaccaaag gcggccgctt ttacatcact    420 

ggagtcttcc aaatccttgc tggtctgtgt gtgatgagtg cagcggccat ctacacagtg    480 

agacacagtg agtggcatgt caacaacgac tactcctatg gctttgctta catcctggcc    540 

tgggtggctt tcccgctggc cctccttagt ggcatcatct acgtgatcct gcggaaacgc    600 

gaatgaggcg cccgacgcac catccgtcta ggctctgagc gtgcataggg tacacaggga    660 

gggaggaagg aaaccagaaa accaaaccaa ccaacccaaa agagctagcc cccaaaccca    720 

aacgcaagcc aaaccaaaca gaacacagtt gagtggggat tgctgtcgat tgaagatgta    780 

tataatatct atggtttata aaacctattt ataacacttt ttacatacat gtacatagga    840 

ttgtttgctt tttatgttga ccgtcagcct cgtgttgaat cttaaacgac tctacatcct    900 

aacactataa ccaagctcag tattttcgtt ttgtttcgtt tttttcatct ttttgttttg    960 

ctcagacata aaaaaaaaaa aaaaatccac gtggccccct ttcatctgaa agcagatccc   1020 

tccctcccat tcaacctcat aggataacca aagtgcgggg acaaacccca gatggccaga   1080 

ggccttcaca ctatgggtga cccagtgaat ttagcaggaa taatccgctg cccgaatcaa   1140 

tgtgtgaagc cctaagcact cacagacgaa acgccctgac cagagccctc tgcgaaacca   1200 

atagctggtg gctgcggaac acttgaccct gaaggcggag tactggggca catgtttaaa   1260 

tgagacgtca gagacaagca atctgtgaaa tggtgctata gatttaccat tccttgttat   1320 

tactaatcat ttaaaccact cactggaaac tcaattaaca gttttatgac ctacagcaga   1380 

acagagaccc gatacaaacg gttcgtaact gctttcgtac atagctaggc tgttgttatt   1440 

actacaataa ataaatctca aagccttcgt cactcccaca gttttctcac ggtcggagca   1500 

tcaggacgag ggtctagacc cttgggacta gcaaattccc tggctttctg ggtctagagt   1560 

gttctgtgcc tccaaggact gtctagcgat gacttgtatt ggccaccaac tgtagatgta   1620 

tatacggtgt ccttctgatg ctaagactcc agacctttct tggttttgct ggctttttct   1680 

gattttatac caactgtgtg gactaagatg cattaaaata aacatcagag taactc       1736 

 
           
             94  
             375  
             PRT  
             Rattus norvegicus  
             
               Cytoplasmic beta-actin  
             
           
            94 

Met Asp Asp Asp Ile Ala Ala Leu Val Val Asp Asn Gly Ser Gly Met 
1               5                   10                  15 

Cys Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val Phe Pro 
            20                  25                  30 

Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly Met Gly 
        35                  40                  45 

Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Arg Gly Ile 
    50                  55                  60 

Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Val Thr Asn Trp Asp 
65                  70                  75                  80 

Asp Met Glu Lys Ile Trp His His Thr Phe Tyr Asn Glu Leu Arg Val 
                85                  90                  95 

Ala Pro Glu Glu His Pro Val Leu Leu Thr Glu Ala Pro Leu Asn Pro 
            100                 105                 110 

Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr Phe Asn 
        115                 120                 125 

Thr Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu Tyr Ala 
    130                 135                 140 

Ser Gly Arg Thr Thr Gly Ile Val Met Asp Ser Gly Asp Gly Val Thr 
145                 150                 155                 160 

His Thr Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala Ile Leu 
                165                 170                 175 

Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met Lys Ile 
            180                 185                 190 

Leu Thr Glu Arg Gly Tyr Ser Phe Thr Thr Thr Ala Glu Arg Glu Ile 
        195                 200                 205 

Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp Phe Glu 
    210                 215                 220 

Gln Glu Met Ala Thr Ala Ala Ser Ser Ser Ser Leu Glu Lys Ser Tyr 
225                 230                 235                 240 

Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly Asn Glu Arg Phe Arg 
                245                 250                 255 

Cys Pro Glu Ala Leu Phe Gln Pro Ser Phe Leu Gly Met Glu Ser Cys 
            260                 265                 270 

Gly Ile His Glu Thr Thr Phe Asn Ser Ile Met Lys Cys Asp Val Asp 
        275                 280                 285 

Ile Arg Lys Asp Leu Tyr Ala Asn Thr Val Leu Ser Gly Gly Thr Thr 
    290                 295                 300 

Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu Ile Thr Ala Leu 
305                 310                 315                 320 

Ala Pro Ser Thr Met Lys Ile Lys Ile Ile Ala Pro Pro Glu Arg Lys 
                325                 330                 335 

Tyr Ser Val Trp Ile Gly Gly Ser Ile Leu Ala Ser Leu Ser Thr Phe 
            340                 345                 350 

Gln Gln Met Trp Ile Ser Lys Gln Glu Tyr Asp Glu Ser Gly Pro Ser 
        355                 360                 365 

Ile Val His Arg Lys Cys Phe 
    370                 375 

 
           
             95  
             4100  
             DNA  
             Rattus norvegicus  
             
               misc_feature  
               3859  
               n is a or g or c or t  
             
           
            95 

acctcttcct caactcactt ctctctactc tcactttttt ttttcttctt cttttttttt     60 

tttttttttt tttttttttt tgcaaaagaa gggggtaaaa aaatgctgca ctgtcgggcg    120 

aggccggtga gtgagcgagc cggagccaat cagcgcccgc cgttccgaaa ttgcctttta    180 

tggctcgagt ggccgctgtg gcgtcctata aaacccggcg gcgcaacgcg cgccactgtc    240 

gagtccgcgt ccacccgcga gtacaacctc cttgcagctc ctccgtcccg gtccacaccc    300 

gccaccaggt aagcagggac gtcgggccca gcgggcccca actttacctt ggccactacc    360 

tcgctgcagg atcgtgagga acactcagaa gggacaccgt agaggggtgg agcgtggtac    420 

cgggccgcgg agcagctggc aaagcttaac tttcccggcc cctagggtgt agagtgtttg    480 

cagtcgtatt cccgcggtgt ggacactcgt gggtacgctc ctgcttggtg cgcggggctt    540 

ggggacacac tagagtcgcg gtgtgggcat ttggagagcc ggtgcggctg cgggtgttaa    600 

gccgcatctg tccaccttga ggggcaacag tattggggtc aggcgttaca atcacgcttt    660 

gatggcctat gggtctttgt ccaaaccggt tttgccattc ggcttggcgg gcgcggcggg    720 

gccgctcggc cgggtggggg ctgggatgcc attgcgcgtg cgcgctctat cactgggcat    780 

tggggccgtg cgcgctgggg agggaactct tcctctcccc ctcttccgag ttaagagttg    840 

cgcgtgcgta ttgagactag gagcgcggcc gccccgggtt gggcgagggc gggccgtcca    900 

ccggaagggg cggggtcgta gcggctaggc gcctgctcgc gcttcctgct gggtgtggtc    960 

gcctcccgcg cgcgcactag ccgcccgtcg cctcagtgta ggcgggcctg tgcccgtttg   1020 

gggaggggcg gaggcctggc ttcctgccgt gggtccgcct ccgggccagc gtttgccttt   1080 

tatggtaata atgcggctgt cctgcgcttc ctttgtcccc tgagcttggg cgcgcccctg   1140 

gcggctcgag gccgcggctc gccggaagtg ggcagggcgg cagcggctgc tcttggcggc   1200 

tccgcggtga ccatagccct cttttgtgcc ttgatagttc gccatggatg acgatatcgc   1260 

tgcgctcgtc gtcgacaacg gctccggcat gtgcaaggcc ggcttcgcgg gcgacgatgc   1320 

tccccgggcc gtcttcccct ccatcgtggg ccgccctagg caccaggtaa gtgacccttt   1380 

actttgggag tggcagccct agggttttct tgggggtcga tgccagtgct gagaacgttg   1440 

ttctcctccg cagggtgtga tggtgggtat gggtcagaag gactcctacg tgggcgacga   1500 

ggcccagagc aagagaggca tcctgaccct gaagtacccc attgaacacg gcattgtaac   1560 

caactgggac gatatggaga agatttggca ccacactttc tacaatgagc tgcgtgtggc   1620 

ccctgaggag caccctgtgc tgctcaccga ggcccctctg aaccctaagg ccaaccgtga   1680 

aaagatgacc caggtcagta tcctgggtga ccctcccctt cttattgggt caacttctca   1740 

gcacgccctt ctctaattgt ctttcttctg ccatgtccca taggactctc ttctatgagc   1800 

tgagtctccc ttggaacttt gcagtttctg ctctttccca gatgaggtct tttttttctc   1860 

tcgatcgcct ttctgactag gtgttttaaa cccctacagt gctgtgggtg taggtactaa   1920 

caatggctcg tgtgacaaag ctaatgaggc tggtgataaa tggccttgga gtgtgtattc   1980 

agtagatgac agtaggtcta aatggagccc ctgtcctgat actcccagca cacttaactt   2040 

agctgtgttc ttgccctctt tgcatgtctc actcaaatct atccttacag tctcacctgc   2100 

cctgagtgtt tcttgtggct ttaggagctt gacaatactg tattcctttc tctacagatc   2160 

atgtttgaga ccttcaacac cccagccatg tacgtagcca tccaggctgt gttgtccctg   2220 

tatgcctctg gtcgtaccac tggcattgtg atggactccg gagacggggt cacccacact   2280 

gtgcccatct atgagggtta cgcgctccct catgccatcc tgcgtctgga cctggctggc   2340 

cgggacctga cagactacct catgaagatc ctgaccgagc gtggctacag cttcaccacc   2400 

acagctgaga gggaaatcgt gcgtgacatt aaagagaagc tgtgctatgt tgccctagac   2460 

ttcgagcaag agatggccac tgccgcatcc tcttcctccc tggagaagag ctatgagctg   2520 

cctgacggtc aggtcatcac tatcggcaat gagcggttcc gatgccccga ggctctcttc   2580 

cagccttcct tcctgggtaa gttgtagtct cgtcccttct ccatctaaag gtgaccaatg   2640 

ctggaggcca cactgtaact ctgatctctt gcttttcctt tcaggtatgg aatcctgtgg   2700 

catccatgaa actacattca attccatcat gaagtgtgac gttgacatcc gtaaagacct   2760 

ctatgccaac acagtgctgt ctggtggcac caccatgtac ccaggcatcg ctgacaggat   2820 

gcagaaggag attactgccc tggctcctag caccatgaag atcaaggtaa gcagccttag   2880 

cctggaccca tagtggggtg tggtcagccc tgtagttgta gccaactctc ttggcttaag   2940 

gaacaaccca gcatccagaa tgctcacaat cactgtcttg ctttcttcag atcattgctc   3000 

ctcctgagcg caagtactct gtgtggattg gtggctctat cctggcctca ctgtccacct   3060 

tccagcagat gtggatcagc aagcaggagt acgatgagtc cggcccctcc atcgtgcacc   3120 

gcaaatgctt ctaggcggac tgttactgag ctgcgtttta caccctttct ttgacaaaac   3180 

ctaacttgcg cagaaaaaaa aaatgagaca tttggcatgg ctttattgtt tttttgtttt   3240 

ttgtttttgt tttttttaaa tttttttttt aaaaaggttt tttttttttg ttttgttttg   3300 

gcgcttttga ctcaaggatt taaaaactgg aacggtgaag gcgaccgcag ttggttggag   3360 

caaacatccc ccaaagttct acaatgtggc tgaggacttt gattgtacat tgttttttgt   3420 

ttttggtttt tttaatagtc actccaagta tccacggcat agatggttac aggaagtccc   3480 

tcaccctccc aaaagccacc cccaactcct aaggggagga tggctgcatc catgccctga   3540 

gtccacaccg ggaaggtgac agcattgctt ctgtgtaaat tatgtacttg caaacatttt   3600 

tttaaatctt ccgccttaat acttcatttt tgtttttaat ttctgaatgg tcagccattc   3660 

gtggcctgcc cctttttttt gtccccccaa cttgatgtat gaaggctttg gtctccctgg   3720 

gagtggtttg aggtgttgag gcgccagggc tggcctgtac actgacgtga gaccgtttta   3780 

ataaaagtgc acaccttaca aacaagtttg tggctctgtg gcttctactg ggtgtgggga   3840 

gcaggctggg tgggtgtgna actccacgtg ggggaggggc aatttagggt gggctggcct   3900 

tgcctgatag ctagtgggag ggctaaagga tcatgatctt taatgaggtc tcataaatac   3960 

cccaccttcg gtcttgaggg agggtgagga gttgggaagg tatctccctg acacctggcc   4020 

aagctggcct catcaaccct actttcctca gccaggggga ggtccacatc ctcccacctg   4080 

catagcttgc gtctagaggc                                               4100 

 
           
             96  
             116  
             PRT  
             Mus musculus  
             
               Ly6/neurotoxin homolog  
             
           
            96 

Met Thr His Leu Leu Thr Val Phe Leu Val Ala Leu Met Gly Leu Pro 
1               5                   10                  15 

Val Ala Gln Ala Leu Glu Cys His Val Cys Ala Tyr Asn Gly Asp Asn 
            20                  25                  30 

Cys Phe Lys Pro Met Arg Cys Pro Ala Met Ala Thr Tyr Cys Met Thr 
        35                  40                  45 

Thr Arg Thr Tyr Phe Thr Pro Tyr Arg Met Lys Val Arg Lys Ser Cys 
    50                  55                  60 

Val Pro Ser Cys Phe Glu Thr Val Tyr Asp Gly Tyr Ser Lys His Ala 
65                  70                  75                  80 

Ser Ala Thr Ser Cys Cys Gln Tyr Tyr Leu Cys Asn Gly Ala Gly Phe 
                85                  90                  95 

Ala Thr Pro Val Thr Leu Ala Leu Val Pro Ala Leu Leu Ala Thr Phe 
            100                 105                 110 

Trp Ser Leu Leu 
        115 

 
           
             97  
             3925  
             DNA  
             Mus musculus  
             
               Ly6/neurotoxin homolog  
             
           
            97 

taccaacacc gcacgaagtg tgtacagatt cccagttaga cagcaggagg gacctgggag     60 

cggccagggg gatgttttat ctctaagaga ccaagagctc aggcagggct tctgtgccct    120 

gcttcctccc tggcttgagc tggatcctgg accagctgct gacctcctgt tcactctggc    180 

actgccctca cgtctccgtc atgacccatc tgctcacagt gttcctggtg gccctgatgg    240 

gcctgcctgt ggcccaggct ctggagtgcc acgtgtgtgc ctacaatgga gacaactgct    300 

tcaaacccat gcgctgccca gccatggcca cctactgtat gaccacacga acttacttca    360 

ccccataccg gatgaaggtg aggaagtcct gtgtccccag ctgctttgaa accgtgtacg    420 

atggctattc caagcatgca tctgccacct cctgttgcca gtactacctc tgcaacggtg    480 

ctggctttgc taccccggtg accttggccc tggtcccagc actcctagct accttctgga    540 

gcttgctgta aagctcggtt ccccaagcca gatccactca aacgcaacac tctcaaaaaa    600 

cacagtttcc ctctctctcc caattcactc cacccaacgc tcttccttct gacactcctc    660 

aactaccacg aggtcccatg gctacctacg aaagaactga tggcatccag atacctcact    720 

ccaaggtcat tttcagaagg ctgacatgtg gacctgtaat gtgcccaccc atgggtgggg    780 

caggctgggc ttctcctcta cccaagatca ggggcatctg ggagaatgtt tatggaggag    840 

gggtcatcac tcaagtcaag gagcactgat ttgatagaat tagtagccaa actccacctt    900 

cagaaccctg cctcagtcta cccagtagag gatgggtctg ctagaggtga ggggaggaga    960 

gcggcggaga ataacgagct ggctagaagc agagaaagac tcagcagggc tgtctccgaa   1020 

gatcagcgcg gcttgccaga gcaaatgtga tgtggaagcc atgtgaggaa gccctttgtc   1080 

atttccactt atctgaggaa ctctgccaga cctgatgttg ggatagccat tggccaaggg   1140 

ttcctagcaa cggcgtcatt tccataggcc actgaaatcc ctccagcccc agctcagcag   1200 

gccccttgac ctccactaca gtccttcatt cacacaccag ctgctgggcc ttgaagttgg   1260 

cagggacttg ggagcaggtg acccatgcta ttttttgtct ggcctgttat tctgggcatg   1320 

gcaagaaggg atcagacgca ggtcagagca gggcagtagg gcgactgaga cagggaaaca   1380 

gacttcagcc agtggcttcc caggtcccgt aggcagctcc tacatccttc agtctcttgt   1440 

tacattcccg ggagacaaat atacagggag ccaagccgag tgctaggtga tgactgcctg   1500 

tgaagtctat tgtggccaca gactgctggg taccaagtct caggagaacc cagcctagat   1560 

ttaggagaca cagatctgcc tttcatgcag tgtagctgtc cttgggagcc ttaccatgct   1620 

ctctaactag ttcctcaact cacatgtcac tgaggaaccc cctaacactg gcccagccca   1680 

ggggtcggga tgctggccaa tgtccatgga gtgggactac ccctggagag tccttgggtc   1740 

atcacatcac aaatgtttta ttccaacctc ccagtggtga gagctcggga cacaaaggtc   1800 

catcctgggg accttcttcc tggttctagg cagacctgaa ctctgtctgc tgctagagct   1860 

gatgtggttt tccgcctcag tttcctcctc cggggatagg ccaccggagg atttgggagg   1920 

gtggggaggg catcctgctg atgggctcgc cgaggttctc aggaacagga acgggcgggg   1980 

ctttagtaca caggtgagtt gggtgggaac tggcccggag ctgaggagac actgactggg   2040 

cagagggaag atgagtctca agggagggca ggaaaaggga gggggagcgc gcatgcacat   2100 

gtgcactcag tgcaggctac agagcccaaa aggcagcact ggctgtggtg tcccctgagg   2160 

cccaggcaag atgctaggag gaagccaatg ctgcccccac ctgagctcac atggaacatg   2220 

cacaccacca gcagcagcag caagcattga gactgacctg tggacgccat agggcactgg   2280 

caaggagggt cagaggcggg tccctgactc agtgggtgag gcccgggaaa cattatcctg   2340 

ttaccctgcg tgtgcaagat cattgtcccc agctagatgg cgtcctcaac caaaactgag   2400 

aggagcccca gttcaggtcc tccctcctac cacaaggggg tggtgtggag gaggcttgat   2460 

tgcccttgga gaagcaccgg tactgcagag ctgggggcca gcttctttca tctgtgtcta   2520 

gacaccgacc agataggccc cacagtggca acactgccac acagtcctac aagaagccct   2580 

gtgcctagct agcacagagc cccaaaaggt gctcaattaa tacagggcca agcctgccag   2640 

tgggggggat gcagattagg ggaacagacc cagatggcct gtcctgaacc ctgtctgggg   2700 

tggtgtgatg agcatctgtc tagcccactg caggtggctc tacacactcc acaacagttc   2760 

tgcaaaagtg tatgaggtgg tcattactgc gcccctctca caggtaaagg cactgaggca   2820 

cggaggagtg aggcacttca ttttcctggg ccattcaact ttccaggacc aacacattca   2880 

actatgggta ctactccaat agctggggtt ctttgaggct gggccccctg aagatgatag   2940 

tggcttcatc aaccagagaa tttcagagtg cagtgttgta ggagcctatg aacctgaaat   3000 

gtcagaactg gaggtttgag gggctgaggg gtaggccagg ggtgtctggc cccttgtgtg   3060 

gagacagaga gagagggaac atgggatggg gtagtagaga gaagtgcaaa ggagcgtcag   3120 

cctttctcag ggctaatgct gtcagggacg agggctcaag cctgtgagtg ttctcacact   3180 

gtgataaaca gtggcccctc aacacagacg gtgtccagag tggccggcag tggttatcta   3240 

gagttgcaat ctggaagcct cttggtagtc actggagaga ggccgcttga tgggacagca   3300 

ccaaatgtgt gtgcttctgt gggatgtgag gaagctgggt cagcgcatga agccaaagcg   3360 

tccttcagag cagaggggtg gctggtctag tccaccagag acaagctatc cagtgagagt   3420 

catactctgt caccgtctct gtgattacct taccccaaag cagacgggga cgggatgcag   3480 

agcacccgtg tcttcatctt ctgcggcaag cacgtgagtt cacattctga aactctagaa   3540 

agatttccag gagtggggtg tgcctttgct ttggtgcatg gttacttcct ggcaagcacc   3600 

gtggcatccc gcagcactga gtgacctggg ctcctcaagc catctcattg gtgaaatgac   3660 

agtgccagta ccctctcagc tggctcttgg aggcctgtgc atggggtctg cacagaggag   3720 

gcccccaaac tatgcatgga cggacacgtg atgcctagca cttcccttgg ttgtgtctct   3780 

gccaacccca ggctctcacc cagcaaggaa atgaaatcca cttttatgac acatctccct   3840 

cccccagcca gctccattca cctatatgcc agggtggtcc ctttcaatgt ctgtccccca   3900 

ttggatgaat aaacaagcga aggac                                         3925 

 
           
             98  
             130  
             PRT  
             Rattus norvegicus  
             
               Astrocytic phosphoprotein  
             
           
            98 

Met Ala Glu Tyr Gly Thr Leu Leu Gln Asp Leu Thr Asn Asn Ile Thr 
1               5                   10                  15 

Leu Glu Asp Leu Glu Gln Leu Lys Ser Ala Cys Lys Glu Asp Ile Pro 
            20                  25                  30 

Ser Glu Lys Ser Glu Glu Ile Thr Thr Gly Ser Ala Trp Phe Ser Phe 
        35                  40                  45 

Leu Glu Ser His Asn Lys Leu Asp Lys Asp Asn Leu Ser Tyr Ile Glu 
    50                  55                  60 

His Ile Phe Glu Ile Ser Arg Arg Pro Asp Leu Leu Thr Met Val Val 
65                  70                  75                  80 

Asp Tyr Arg Thr Arg Val Leu Lys Ile Ser Glu Glu Asp Glu Leu Asp 
                85                  90                  95 

Thr Lys Leu Thr Arg Ile Pro Ser Ala Lys Lys Tyr Lys Asp Ile Ile 
            100                 105                 110 

Arg Gln Pro Ser Glu Glu Glu Ile Ile Lys Leu Ala Pro Pro Pro Lys 
        115                 120                 125 

Lys Ala 
    130 

 
           
             99  
             2341  
             DNA  
             Rattus norvegicus  
             
               Astrocytic phosphoprotein  
             
           
            99 

gcggaagagg cggcggcggc aggatcggcg gcagcggtgg ccggagccaa ggagcaggct     60 

ccagacgctg cttaggaacc ggggatccgg gagtgcccgc accctgagct ctcagctccg    120 

gaggcgtcat ggcagagtac gggactctcc ttcaggacct gaccaacaac atcacccttg    180 

aagatctgga acagctcaag tcagcctgca aggaggacat ccccagtgag aagagtgaag    240 

agatcactac aggcagtgcc tggtttagct tcctggagag ccacaacaag ctggacaaag    300 

acaacctctc ctacattgag cacatctttg agatctcccg ccgtcctgac ctactcacga    360 

tggtggttga ctacagaacc cgtgtgctga agatctctga ggaggatgag ctggacacca    420 

agctaacccg tatccccagc gccaagaagt acaaagacat tatccggcag ccctctgagg    480 

aagaaatcat caaattggct cccccaccaa agaaggcctg agcaaggggg aggaagagga    540 

ggaaggttgg atcttcatca gaccactccc ttccccatcc tcaatgggag gggctagggc    600 

aacaccctgc tccgtaccca cttactaact tggtcctaac ccttactatg cgcgtgtgtg    660 

tgcgtgtgcg cacgctctgg ctgtctgtct gtctagctca tctagttcct cctcctcatg    720 

aggggattgg agtcaaggga ggggggctta gctgccccca ctgtaagggg aggtggacgc    780 

tttttctatg taaacagaaa ctggcacact cctcctcctc tcctctcctc cactcttccc    840 

cacatcttta aagtatggaa gcagaaaggg cctgcgtttt cccacactga ggagctatgg    900 

tgaaaatgag gtatggcgga aatgattaga tctaaagaaa accagtgggg aaggaaggtc    960 

aagaggctac ccaacctcca cctgaagggg ctcctgagtg ccagcgggcc ctcctttatg   1020 

cccttgttac gggcctggga tgtggataag cagagatcct aatgagaaga ccactagctc   1080 

ctttcctagg tcccaagatc aaaccccagt ggagaagcca gcttttgatg tccccaactt   1140 

ctgctctgga gagactatgc tgggaccatg cactgctgag cctgagccag ggatgcaggc   1200 

agagacaggc ccactctcgc tcctcagttt ctagatacag actgttggga tatactgacc   1260 

cggagcctag gaattgggga atgagatccc ttaggtttta accgcaacct gcagagtccc   1320 

aaaatgggta ttgtaggcaa cctttccact ttccagttcg gaattctcca agcaagagat   1380 

aagttaacga cagtgtttcc tttgcactga ctaccacccc attcaatcca ggaagggact   1440 

ggtcaccttt ctcatttggg tttgtgaatg ccacacagcc aagccactag agtacagtga   1500 

gtgaacccag cctcctccct gtcccaagat gcccttcccc atcttgaccg tgctaactgt   1560 

gtgtacatat atattctaca tatatgtata ttaaacccgc actgccatgt ctgccctttt   1620 

ttgtggtttt tagcattaac ttattgtcta ggccagagcg ggagtgggag gggaatgcca   1680 

cagtaaaggg agtggcagag ccaaattgct aagttagtca aaacagaaaa catttccaac   1740 

caatcacaac aaacattgca tttacatttg agctaagaga caatttagag aaagttgaag   1800 

gtaggagctt ttaggatgtg ggagcaaaac ttcacaggga agggaggacc agctgtcaga   1860 

agagggctct tgaagccaga ctggctagag caccctcact tcctagctga gcccagtttg   1920 

cccatctcct ctggccactg ctgcagacac ctgccttaac acacacacct ctaagactcc   1980 

agttttgcct taaaggccct tcctaagtct ccctagtcct gcctggcatc tcctttgaga   2040 

caagaaatcc ttgtacaact gggagaaagg aactatgggc aacatccttc catctggggt   2100 

gttacgtgag agcaggagtt aagacggagg ctttccttat gaagacacag gagagagaga   2160 

gggtgcttct cagcagtttc tctgtaaggc aaaaaacaca aaccttaaac acactaacct   2220 

gccctaatac actcacctac cctgctaccc tcgtgctgct gtgaggttgc ccctagcctg   2280 

tgttcctctg tctgcagcgt gcacggcctt gttctaaccc tggaataaag gtgactgact   2340 

g                                                                   2341 

 
           
             100  
             582  
             PRT  
             Mus musculus  
             
               PLIC-1  
             
           
            100 

Met Ala Glu Ser Ala Glu Ser Gly Gly Pro Pro Gly Ala Gln Asp Ser 
1               5                   10                  15 

Ala Ala Asp Ser Gly Pro Ala Glu Pro Lys Ile Met Lys Val Thr Val 
            20                  25                  30 

Lys Thr Pro Lys Glu Lys Glu Glu Phe Ala Val Pro Glu Asn Ser Ser 
        35                  40                  45 

Val Gln Gln Phe Lys Glu Glu Ile Ser Lys Arg Phe Lys Ser His Ile 
    50                  55                  60 

Asp Gln Leu Val Leu Ile Phe Ala Gly Lys Ile Leu Lys Asp Gln Asp 
65                  70                  75                  80 

Thr Leu Ser Gln His Gly Ile His Asp Gly Leu Thr Val His Leu Val 
                85                  90                  95 

Ile Lys Thr Gln Asn Arg Pro Gln Asp Asn Ser Ala Gln Gln Thr Asn 
            100                 105                 110 

Thr Thr Gly Asn Ser Val Thr Ser Ser Pro Ala Pro Asp Ser Asn Pro 
        115                 120                 125 

Thr Ser Gly Pro Ala Ala Asn Ser Ser Phe Gly Leu Gly Gly Leu Gly 
    130                 135                 140 

Gly Leu Ala Gly Leu Ser Ser Leu Gly Leu Asn Thr Thr Asn Phe Phe 
145                 150                 155                 160 

Glu Leu Gln Ser Gln Met Gln Arg Gln Leu Leu Ser Asn Pro Glu Met 
                165                 170                 175 

Met Val Gln Ile Met Glu Asn Pro Phe Val Gln Ser Met Leu Ser Asn 
            180                 185                 190 

Pro Asp Leu Met Arg Gln Leu Ile Met Ala Asn Pro Gln Met Gln Gln 
        195                 200                 205 

Leu Ile Gln Arg Asp Pro Glu Ile Ser His Met Leu Asn Asn Pro Asp 
    210                 215                 220 

Ile Met Gly Gln Thr Leu Glu Leu Ala Arg Asn Pro Ala Met Met Gln 
225                 230                 235                 240 

Glu Met Met Arg Asn Gln Asp Arg Ala Leu Ser Asn Val Glu Ser Ile 
                245                 250                 255 

Arg Gly Gly Tyr Asn Ala Leu Arg Arg Met Tyr Thr Asp Ile Gln Glu 
            260                 265                 270 

Pro Met Ile Asn Ala Ala Gln Glu Gln Phe Gly Gly Asn Pro Phe Gly 
        275                 280                 285 

Ser Leu Val Ser Ser Pro Ser Ser Ala Glu Gly Thr Gln Pro Ser Arg 
    290                 295                 300 

Thr Glu Asn Arg Asp Pro Leu Pro Asn Pro Trp Ala Pro Gln Thr Pro 
305                 310                 315                 320 

Gln Ser Ser Pro Ala Tyr Gly Ser Ser Gly Ser Thr Asn Asn Thr Val 
                325                 330                 335 

Ser Thr Ser Gly Gly Asn Ala Thr Ser Thr Pro Ala Gly Gln Gly Thr 
            340                 345                 350 

Ser Gly Pro Lys Leu Val Pro Gly Ala Gly Gly Ser Met Phe Asn Thr 
        355                 360                 365 

Pro Gly Met Gln Ser Leu Leu Gln Gln Ile Thr Glu Asn Pro Gln Leu 
    370                 375                 380 

Met Gln Asn Met Leu Ser Ala Pro Tyr Met Arg Ser Met Leu Gln Ser 
385                 390                 395                 400 

Leu Ser Gln Asn Pro Asp Leu Ala Ala Gln Met Met Leu Asn Asp Pro 
                405                 410                 415 

Leu Phe Ala Gly Asn Pro Gln Leu Gln Glu Gln Met Arg Gln Gln Leu 
            420                 425                 430 

Pro Thr Phe Leu Gln Gln Met Gln Asn Pro Asp Thr Leu Ser Ala Met 
        435                 440                 445 

Ser Asn Pro Arg Thr Met Gln Ala Leu Leu Gln Ile Gln Gln Gly Leu 
    450                 455                 460 

Gln Thr Leu Ala Thr Glu Ala Pro Gly Leu Ile Pro Gly Phe Thr Pro 
465                 470                 475                 480 

Gly Leu Ala Ala Gly Asn Ser Gly Gly Pro Ala Gly Thr Thr Ala Pro 
                485                 490                 495 

Ser Thr Ala Pro Gly Glu Asp Thr Asn Pro Gln Gly Gly Ala Ala Glu 
            500                 505                 510 

Pro Gly His Gln Gln Phe Ile Gln Gln Met Leu Gln Ala Leu Ala Gly 
        515                 520                 525 

Val Asn Pro Gln Leu Gln Ser Pro Glu Val Arg Phe Gln Gln Gln Leu 
    530                 535                 540 

Glu Gln Leu Ser Ala Met Gly Phe Leu Lys Arg Glu Pro Asn Leu Gln 
545                 550                 555                 560 

Ala Leu Ile Ala Thr Gly Gly Asp Ile Asn Ala Ala Ile Glu Arg Leu 
                565                 570                 575 

Leu Gly Ser Gln Pro Ser 
            580 

 
           
             101  
             1843  
             DNA  
             Mus musculus  
             
               PLIC-1  
             
           
            101 

ccatggccga gagcgcagag agcggcggcc caccgggcgc tcaggacagt gcggccgaca     60 

gcggccccgc agagcccaag atcatgaagg tcacggtgaa gacgcctaaa gagaaggaag    120 

agttcgctgt gcccgagaac agctcggtcc agcagttcaa agaggaaatc tcaaaacgtt    180 

tcaaatctca tattgaccaa cttgtgttga tatttgctgg aaaaatttta aaagatcaag    240 

atactttgag tcagcatggg attcatgatg gacttacggt tcaccttgtc atcaaaaccc    300 

aaaacaggcc gcaagataat tcagctcagc aaacaaatac cactggaaac agtgtgacca    360 

gttcaccagc tcctgacagt aaccccacgt ctgggcctgc tgctaacagc tcctttggtt    420 

taggtggact tggaggactt gcaggtctta gtagcttggg tttaaatacc accaacttct    480 

ttgaactaca gagccaaatg cagcggcaac ttttgtctaa ccctgaaatg atggtccaga    540 

tcatggaaaa cccctttgtg cagagcatgc tctcaaaccc tgacctgatg cggcagctga    600 

tcatggccaa cccacagatg cagcagctga tacagagaga cccagagatc agtcatatgc    660 

ttaataaccc agatataatg ggacagacat tggaacttgc cagaaaccca gcgatgatgc    720 

aggaaatgat gagaaaccag gaccgagcct tgagcaacgt agaaagtatc cgtggagggt    780 

acaacgcctt acggcgcatg tacacagata ttcaggagcc catgataaac gctgcgcaag    840 

agcagtttgg tggtaatcca tttggttcct tagtgagcag tccgtcctca gcagaaggga    900 

ctcagccttc tcgaacagaa aatagggatc cgctacctaa cccttgggct ccacagactc    960 

cccagagctc tccagcctat ggcagcagtg gcagcaccaa taacacagtg agcacctctg   1020 

gtggcaatgc caccagtacc ccggcgggac agggcacctc agggcccaag cttgtgcctg   1080 

gagcaggagg tagtatgttc aacacccccg gaatgcagag cctgctgcag cagataactg   1140 

agaacccaca gctcatgcag aacatgctgt ctgccccata catgaggagc atgctgcagt   1200 

ccctgagcca gaaccccgac cttgctgcgc agatgatgct gaatgacccc ctatttgctg   1260 

ggaaccctca gcttcaagag caaatgagac aacagctccc aacgttcctc caacaaatgc   1320 

agaaccccga cacattgtca gccatgtcga accctagaac catgcaggcc ctgctgcaaa   1380 

tccagcaggg cttgcagacg ttggccacag aagctcccgg cctcatccca gggtttactc   1440 

ctggcttggc agcgggcaat tctggaggcc ctgcaggaac cactgcacct agcactgcac   1500 

ctggtgagga tacgaacccc caagggggcg ctgctgagcc aggccaccag cagtttatcc   1560 

agcaaatgct gcaggccctc gccggggtga accctcagct gcagagtcca gaagtcagat   1620 

ttcaacagca actggaacag ctcagtgcaa tgggattctt gaagcgtgaa ccaaacttac   1680 

aagcactgat agcaacaggg ggcgacatca atgcggccat tgaaaggttg ctgggttccc   1740 

agccgtcata gcagcatttc tgtatcctga aaaaatgtaa tttatttttg ataacggctc   1800 

ttaaatcttt aaaataacct gctttatttc attgggattc tgt                     1843 

 
           
             102  
             618  
             PRT  
             Rattus norvegicus  
             
               Nfxl  
             
           
            102 

Met Ala Asp Glu Gly Lys Ser Tyr Asn Glu His Asp Asp Arg Val Ser 
1               5                   10                  15 

Phe Pro Gln Arg Arg Lys Lys Gly Arg Gly Pro Phe Arg Trp Lys Cys 
            20                  25                  30 

Gly Val Gly Asn Arg Arg Ser Gly Arg Gly Gly Ser Gly Ile Arg Ser 
        35                  40                  45 

Ser Arg Phe Glu Glu Asp Asp Gly Asp Val Ala Met Asn Asp Pro Gln 
    50                  55                  60 

Asp Gly Pro Arg Val Arg Phe Asn Pro Tyr Thr Thr Arg Pro Asn Arg 
65                  70                  75                  80 

Arg Arg Asp Thr Trp His Asp Arg Asp Arg Ile His Val Thr Val Arg 
                85                  90                  95 

Arg Asp Arg Ala Pro Gln Glu Arg Gly Gly Ala Gly Thr Ser Gln Asp 
            100                 105                 110 

Gly Thr Thr Lys Asn Trp Phe Lys Ile Thr Ile Pro Tyr Gly Lys Lys 
        115                 120                 125 

Tyr Asp Lys Met Trp Leu Leu Ser Met Ile Gln Ser Lys Cys Ser Val 
    130                 135                 140 

Pro Phe Asn Pro Ile Glu Phe His Tyr Glu Asn Thr Arg Ala His Phe 
145                 150                 155                 160 

Phe Val Glu Asn Ala Thr Thr Ala Ser Ala Leu Lys Ala Val Asn Tyr 
                165                 170                 175 

Lys Ile Gln Asp Arg Glu Asn Gly Arg Ile Ser Ile Ile Ile Asn Ser 
            180                 185                 190 

Ser Ala Pro Pro Tyr Ile Val Gln Asn Glu Leu Lys Pro Glu Gln Val 
        195                 200                 205 

Glu Gln Leu Lys Leu Ile Met Ser Lys Arg Tyr Asp Gly Ser Gln Gln 
    210                 215                 220 

Ala Leu Asp Leu Lys Gly Leu Arg Ser Asp Pro Asp Leu Val Ala Gln 
225                 230                 235                 240 

Asn Ile Asp Val Val Leu Asn Arg Arg Gly Cys Met Ala Ala Ala Leu 
                245                 250                 255 

Arg Ile Ile Glu Glu Asn Ile Pro Glu Leu Leu Ser Leu Asn Leu Ser 
            260                 265                 270 

Asn Asn Arg Leu Tyr Lys Leu Asp Asp Met Ser Ser Ile Val Gln Lys 
        275                 280                 285 

Ala Pro Asn Leu Lys Ile Leu Asn Leu Ser Gly Asn Glu Leu Lys Ser 
    290                 295                 300 

Glu Trp Glu Leu Asp Lys Ile Lys Gly Leu Lys Leu Glu Glu Leu Trp 
305                 310                 315                 320 

Leu Asp Arg Asn Pro Met Cys Asp Thr Phe Leu Asp Gln Ser Thr Tyr 
                325                 330                 335 

Ile Ser Thr Ile Arg Glu Arg Phe Pro Lys Leu Leu Arg Leu Asp Gly 
            340                 345                 350 

His Glu Leu Pro Pro Pro Ile Ala Phe Asp Val Glu Ala Pro Thr Met 
        355                 360                 365 

Leu Pro Pro Cys Lys Gly Ser Tyr Phe Gly Thr Glu Asn Leu Lys Ser 
    370                 375                 380 

Leu Val Leu His Phe Leu Gln Gln Tyr Tyr Ala Ile Tyr Asp Ser Gly 
385                 390                 395                 400 

Asp Arg Gln Gly Leu Leu Asp Ala Tyr His Asp Gly Ala Cys Cys Ser 
                405                 410                 415 

Leu Ser Thr Pro Ser Asn Pro Gln Asn Pro Val Arg His Asn Leu Ala 
            420                 425                 430 

Lys Tyr Phe Asn Asp Ser Arg Asn Val Lys Lys Ile Lys Asp Thr Thr 
        435                 440                 445 

Thr Arg Phe Arg Leu Leu Lys His Thr Arg Leu Asn Val Val Ala Phe 
    450                 455                 460 

Leu Asn Glu Leu Pro Lys Thr His His Asp Val Asn Ser Phe Val Val 
465                 470                 475                 480 

Asp Ile Ser Ala Gln Thr Ser Thr Leu Leu Cys Phe Ser Val Asn Gly 
                485                 490                 495 

Val Phe Lys Glu Val Asp Gly Lys Ser Arg Asp Ser Leu Arg Ala Phe 
            500                 505                 510 

Thr Arg Thr Phe Ile Ala Val Pro Ala Ser Asn Ser Gly Leu Cys Ile 
        515                 520                 525 

Val Asn Asp Glu Leu Phe Val Arg Asn Ala Ser Pro Glu Glu Ile Gln 
    530                 535                 540 

Arg Ala Phe Ala Met Pro Ala Pro Thr Pro Ser Ser Ser Pro Val Pro 
545                 550                 555                 560 

Thr Leu Ser Gln Glu Gln Gln Asp Met Leu Gln Ala Phe Ser Thr Gln 
                565                 570                 575 

Ser Gly Met Asn Leu Glu Trp Ser Gln Lys Cys Leu Gln Asp Asn Asn 
            580                 585                 590 

Trp Asp Tyr Thr Arg Ser Ala Gln Ala Phe Thr His Leu Lys Ala Lys 
        595                 600                 605 

Gly Glu Ile Pro Glu Val Ala Phe Met Lys 
    610                 615 

 
           
             103  
             2304  
             DNA  
             Rattus norvegicus  
             
               Nfxl  
             
           
            103 

attccatcct aatacgactc actatagggc tcgagcggcc gcccgggcag gtccaaggcg     60 

tccgggtgtt tggtggcgtc aggttctgcg cctgagcccg cccctgtacc tgcctgtgcc    120 

atggcggacg aagggaagtc atacaacgaa catgatgacc gtgttagttt cccacaaaga    180 

agaaagaaag gtcgggggcc tttccgatgg aagtgtggtg tggggaatcg taggtctgga    240 

agaggtggtt ctggtatacg gtcttcccgg tttgaggaag atgatggaga tgtggcaatg    300 

aatgatcccc aggatggccc ccgagtgaga ttcaacccct ataccacccg acctaaccgt    360 

cggagagata cttggcatga tcgagatcgg attcatgtta ctgtacggag agacagagct    420 

cctcaagaaa gaggaggtgc tggcaccagt caagatggga ccaccaagaa ctggttcaag    480 

attacaattc cttatggcaa aaagtatgat aaaatgtggc ttctgagcat gattcaaagc    540 

aagtgcagtg tccccttcaa ccccattgag tttcactatg aaaatacacg ggcccatttt    600 

tttgtggaaa atgccactac cgcttctgca ttaaaggctg tcaactataa gattcaggat    660 

cgagaaaacg gaaggatatc tatcatcatc aactcttctg ctccacccta tattgtacag    720 

aatgaactga agcctgaaca agtagagcag ctcaagctga tcatgagcaa acgatatgat    780 

ggctcccagc aagcacttga cctcaagggc ctccgctcag acccagattt ggtggcccag    840 

aacatcgatg ttgttctaaa ccgtagaggc tgtatggcag ccgccctgag gatcattgaa    900 

gagaacattc ctgagctatt gtctttgaac ttgagtaaca acaggctgta caagcttgat    960 

gacatgtcca gcattgtaca gaaggcacca aacttaaaga tcctgaatct ctctggaaac   1020 

gaattgaagt ccgagtggga attagacaag ataaaagggc tgaagctgga agagctgtgg   1080 

cttgacagaa accccatgtg tgacaccttc ctagaccagt ccacctatat cagcaccatt   1140 

cgcgaacgat ttcccaagtt attacgcctg gatggccatg agctaccccc tccaattgcc   1200 

tttgatgtcg aagcccctac tatgttacca ccctgcaagg gaagctattt tggaacagag   1260 

aacctaaaga gtctggtcct gcacttcttg cagcagtact atgcaattta tgactcagga   1320 

gaccgccagg gtcttctgga tgcctaccat gatggcgcct gttgctcgct aagcactcct   1380 

tccaaccctc agaaccctgt ccgacacaac ttagccaaat acttcaatga cagcagaaat   1440 

gtgaagaaga ttaaagatac caccacgcgg ttccggctat taaagcatac acgtcttaat   1500 

gtcgttgcct tcctcaatga gttacctaaa actcaccatg acgttaactc ctttgtggta   1560 

gatataagtg cccaaacaag cacattgttg tgtttttctg tcaatggagt cttcaaggaa   1620 

gtggacggga agtctcgaga ttctttacgt gctttcacga gaacgttcat tgctgttcct   1680 

gccagcaatt cagggctatg tattgtaaat gacgaattat ttgtgcggaa cgccagccct   1740 

gaagaaatcc aaagagcctt cgccatgcct gcacccacac cttcttccag cccagtaccc   1800 

acactctccc aagaacagca ggatatgtta caagcatttt ctacccagtc tggcatgaac   1860 

ctcgagtggt ctcagaagtg ccttcaagac aataactggg actacaccag atctgcccag   1920 

gccttcactc atctcaaggc caagggagag attccagaag tagcgtttat gaaatgatct   1980 

caagaaaagt ccccttgtaa atagcccttg gataatattg tctggctgtc atctgcggac   2040 

tcctgtccag cccgaggcca cmctgtaact gtgactgggg aggaagggct gcytcatcck   2100 

tcctcaccta ccttctggaa gmcttagcag aagacttccg gaagattgag cctctatggt   2160 

gccaggaagc caaagcttac tttgtagagc tdgacrctaa attaccggaa ggacttaggt   2220 

gctctgtgta cttaacccca gsattccctt tactttttaa gataaagagt gatattgtag   2280 

tgtgtgtccc aaaaaaaaaa aaaa                                          2304 

 
           
             104  
             175  
             PRT  
             Rattus norvegicus  
             
               Alpha B-crystallin  
             
           
            104 

Met Asp Ile Ala Ile His His Pro Trp Ile Arg Arg Pro Phe Phe Pro 
1               5                   10                  15 

Phe His Ser Pro Ser Arg Leu Phe Asp Gln Phe Phe Gly Glu His Leu 
            20                  25                  30 

Leu Glu Ser Asp Leu Phe Ser Thr Ala Thr Ser Leu Ser Pro Phe Tyr 
        35                  40                  45 

Leu Arg Pro Pro Ser Phe Leu Arg Ala Pro Ser Trp Ile Asp Thr Gly 
    50                  55                  60 

Leu Ser Glu Met Arg Met Glu Lys Asp Arg Phe Ser Val Asn Leu Asp 
65                  70                  75                  80 

Val Lys His Phe Ser Pro Glu Glu Leu Lys Val Lys Val Leu Gly Asp 
                85                  90                  95 

Val Ile Glu Val His Gly Lys His Glu Glu Arg Gln Asp Glu His Gly 
            100                 105                 110 

Phe Ile Ser Arg Glu Phe His Arg Lys Tyr Arg Ile Pro Ala Asp Val 
        115                 120                 125 

Asp Pro Leu Thr Ile Thr Ser Ser Leu Ser Ser Asp Gly Val Leu Thr 
    130                 135                 140 

Val Asn Gly Pro Arg Lys Gln Ala Ser Gly Pro Glu Arg Thr Ile Pro 
145                 150                 155                 160 

Ile Thr Arg Glu Glu Lys Pro Ala Val Thr Ala Ala Pro Lys Lys 
                165                 170                 175 

 
           
             105  
             6806  
             DNA  
             Rattus norvegicus  
             
               Alpha B-crystallin  
             
           
            105 

gggaagagca gcctatgact ttctgcccac acggacgcca ttcggtctac caggttacag     60 

acaggagcag cacaaacaag gcccctagat tttggtttat ttagcctcac ccagaattaa    120 

atcgaaggct acgattcatc aagggaccaa accatacact gtggaccatg cacctatccc    180 

atctcccagg aatgctgctc cctctggggt gtggtggatt tctggaatca ccacgggagg    240 

tagacagggg attcaccggg ttctgggtct ggcagtcagg gttcaactct ggctacctct    300 

tcctcttcct cggggtcagg aggtgcagga agcagggaga tgtagacctc attgacttcc    360 

gtgtccagat gccggccacc ccgcggtgcc tccaagttaa ggatgccatc atgggagaga    420 

gcagctctga cccgccaggg gtccacatct gcaggcagga cataggttgc gacagaactc    480 

tcgggacacg aagccgtggc gtccagacgt tgggggtgtc gggcagacac tctagcaggt    540 

tgtccacagt cctcacggtc acctcatctg gggtaaagtg gctcacatcc agaaatgcct    600 

ggaacttgcc ttcactgagc ctgagctctg aggcccctgc cctgccgccc tccccagctc    660 

ctggcggccc ggggccgaac atagtagcca tggtagaggg tgggggtcag gatctcttct    720 

ggcaggaggc ctgggagcag agaaagaaag agaaggcaag aggcaggatg aggaagaatg    780 

cagcgggcac agacagggct gaaaggcgat atctggagat atgggctaga agagcaggtg    840 

ggaaactgta gaggggctgg gaaaggagag gagatggtta cggaaaaagc gagctggaac    900 

tggaggacag aagcaaaacc agaaaatact ctgaagcgtg caaggcccgg acaccgaaag    960 

ccctgccgat ctaaagtttg gtcctgaagt ctgttagcaa gaccagagag agtcttgctt   1020 

gaggggggag tctggggtgg gagatggctc acctcaggag ttcacttaag ccccaggtag   1080 

agtggaggcc agtgaagctg ggcagtgagc ccagaatttc cggaggtggg ggtaggaagc   1140 

atctgagcca taccttctcc gaagcgctgc tcgccccagg cggctagggt tggcaaattc   1200 

gtactcggca gtggctgggt gggcgtgtgg cactgtgcgg cccgacatga ctgctgttgc   1260 

gactagtagc cctgatccca agtgtagccc caacaagatc aaggcgaccc ctccagcttc   1320 

agagggagag ccaggactca gtcagagtgg gggcggggtc tacaccaccc aaaatagtgc   1380 

agagcgtctg ggggatgggg gaggggtggg agcctaagtc tagagtgcag ctatgagggt   1440 

gtgatcagcg ctgcctggac cccctaagtc ccctcctctg tatacccttc agctgtcgca   1500 

taggcctgga agaacagctg agggtcctgg tcctagagta agctgggaag agaaactggt   1560 

gcgctgcagg agggcaagga gaggcctggt tgggcccttc accaattgta cattccacat   1620 

caccctttgt ccttatcagt ctcaggcact gagcacatta cttagaaaaa aaaaaaaaaa   1680 

gacaatgttc tatgagccac agaacgtcaa aaatacaaga aacattttct gtctttttaa   1740 

tgtcagggtc ttctgaacct agatcaactc ggggttccag tcagacacct agttctgaca   1800 

tattggtggt cacagctctc ctctgggact ccacaaagag ttaatgtccc tgggctcagc   1860 

ccaggaagat tccagcctct gcccaggccc aagatagttg ctggctcaat tcccctggca   1920 

tgcaagactg gagaggagga ggggcccacc agcagctgct tgggattcca gaccctgtcc   1980 

tggctccaga gaacaaggat ggggtgggtg ggtgccacta ggtgtggaca gagagctagt   2040 

gaaacaagac cgtgacaagt caccggccag ctcagccctg ccccgtgttt ctcttttctt   2100 

agctcagtga gtactgggta tgtgtcaccc tgccaaatcc ctgatcacaa gtccccatga   2160 

actgtcgggg agctgggata ataaaacccc tgacatcacc gttccagaag cttcacaaga   2220 

ctgcatatat aaggggcagg ctgtagcagc ggctgaagga gttgaccggc taaccgactc   2280 

tacactcatc tagccatcat ggacatagcc atccaccacc cctggatccg gcgtcccttc   2340 

tttcctttcc actccccaag ccgcctcttt gaccagttct tcggagagca cctgttggag   2400 

tctgacctct tctctacagc cacttccctg agccccttct accttcggcc accctccttc   2460 

ctgcgggcac ctagctggat tgacactggg ctctcagagg taagtctccc ttgtgccagg   2520 

gcaggaatac gtatccttcc tggtctctcc attcctttgc cctttcaccc tgcctaagtc   2580 

attttaggca tgtgcgtgtg caaaggtaca gagaaatgag gttactgttc tcttcctgtg   2640 

gcacctgttc tgttgatgct cctgttcctg gtccattttc tgtgcatggt gcctctttct   2700 

cctctttcaa ctcctttacc cacctcttta atagtcccag tcctggcaga ttttatcatc   2760 

tcaaactgta gagatcagat agaaacccac tcattctggg cttttcctgt cttctttcct   2820 

ccccacttcc ttttgatgga gctggataca tcaatcaata ttctctctct ctctctctct   2880 

ctctctctct ctctctctct ctctctcttc cttggcctat gactttccgg gttcctcggg   2940 

gctgagacag tgcattgact tcagtccatg ccttaagagg accgctcttg ggaagccctg   3000 

acttcttatc tcagcagcgt gggagataag attaacagag gttgtcacag ataacactgt   3060 

ggtttaaaaa tattcgcgtg tgaggaaaca ggagctgagt gagcaagggc tttggaagga   3120 

cagaacattc cagatcgagt gggttggaaa cttggcaggc aactgaacaa gaaaaagggg   3180 

gcttttgttt tatagacaaa tgacaaagcc agcactgggt agagaggcag cagatgccat   3240 

ccccagaccc attcttgtga ctagtctcct actgacccgt cttcttccct gtgcctctcg   3300 

gtaaagtttg ggactgatgg atgactaaga ggtgtctttt gggtatcacc atggtggctc   3360 

acatatacca gtgtcactag tcaataagag gatttttccc ccagcagcca ggtagaggat   3420 

ggattaccag ggcaggaagt aaatctcctg gacgatgagc cttctccagc ttaatggaca   3480 

ttctaccatc ttttctcctc cgcccccttg ttattctaga tgcgtatgga gaaggacagg   3540 

ttctctgtga acctggacgt gaagcacttc tctccagagg aactcaaagt caaggttctg   3600 

ggagacgtga ttgaggtgca cggcaagcac gaagagcgcc aggtgtgtgg acctttctgc   3660 

cttctcttgt gaattcattt agtgccctgc tggatgccag ggactgccgc caccagcctc   3720 

tgaggctggc agaattccag tccccaaaca taagactaga tcaggagtta tagatcaatg   3780 

gctgggaaac aagttagggc tagatctgct gttagtgttg tggctgtgct gagagaactt   3840 

tcccctggat gttccttttg attgccttgt cttgtccagt gacgtcagaa tagcagaaga   3900 

attctcaaca ttcccaaccc tcagcacatc ctaggccaat tgaatctgaa gttttaggga   3960 

catagctcag gcatcaacct ttaaacaaac aaacaaacaa actcactcct tgggtacttt   4020 

ctgtacccca aactggcagc cattgctcta ggcacagtgt gctgtacata atggctctgc   4080 

gaaatgacag tcaccacgga gtcgaaggca ataaaatctg tggcttttga atgttcagga   4140 

ttccatgcct cttctgctca gtttaatgct atgctacttg gtttgtctgc ttttcagagc   4200 

ttcaggctta tggctgtgag gaagttaatt gagatttgtt tgtgtggatg cttggggtac   4260 

ttgggattca ataggtaaca ggattagcat ccacaattcc tcttgcattt ttagcatttt   4320 

tttttcttga cttcctaaaa aggtgttatg attgcttctt aaaaattaat gacttggggg   4380 

gctggggatt tagctcagtg gtagagcgct tacctaggaa gcgcaaggtc ctgggttcgg   4440 

tccccagctc cgaaaaaaaa gaaccaaaaa aaaaaaaatt aatgacttgg ggttggggat   4500 

ttagctcagc ggtagagcgt ttgcctagca agcgcaaggc cctgggttca gtccccagct   4560 

ccgaaaaaaa gaaaagaaaa aaaaattaat gacttagggc ctggagagat ggctcagcag   4620 

ttaaaagcac tgaccgctct tccagaggtc ctgcatacaa ttcccagcaa ccacatggtg   4680 

gctcacaacc atctgtaatg ggatccaatg ccctcttctg gcatgcaggt gtacatgcag   4740 

atagagtgct catacataaa attaaaaaaa tcttaacaaa aaataactca gctgtgccgt   4800 

ggtggtgcat gcctttaatc ccaacatttg ggaggcagag gtagacagat ctaagttaga   4860 

ggccagctta gactacagaa tgagttccag gagagcagga gctcaacaga gaaaccctac   4920 

ttatctcaaa caaaacaaaa aacaaaaatc aatacctcgg ttttgctcat gatttaggac   4980 

tggaccacag tgtcactgat agggtcacct tatataatat cagcaatatt atactagaaa   5040 

cattctgaga cattcctgag caattagaac aatttatggg cttcctataa gatacagaca   5100 

tactcggggt tggggatttg gctcagtggt agagcgtttg cctaggaagc gcaaggtcct   5160 

gggttcggtc cccagccctg aaaaaaagaa aaaagaaaaa aaaaaaagat acagacatac   5220 

tcaatgccaa agtgggagtc taaattccat ggtccagtca acatggattc attcctttgt   5280 

ggaaatgccc tgaattgtag ttgtgtctcc gaatatgaag atctatgaaa ttcggggctg   5340 

tttcagattt cctacactct gattggtagg tgtgtccatc tggacagttt attctagcct   5400 

taaacctgga caaacagctc tggacttagc tatctgcata tttacagtgt tggaggtgaa   5460 

ctcaggcctt tcactactag actgaatccc cagctccaga tattttttcc ccccttaggg   5520 

ccttttgttc taacccaagg gcaggggatg tctagagtct ggggcaagtg gtggtatttc   5580 

ctgctcttat ttctctccct tgcccttcat tctctcggat taggacgaac atggcttcat   5640 

ctccagggag ttccacagga agtaccggat cccagccgac gtggatcctc tcaccattac   5700 

ttcttccctg tcatcggatg gagtcctcac tgtgaatgga ccaaggaaac aggcctctgg   5760 

ccctgagcgc accattccca tcacccgtga agagaagcct gctgtcactg cagcccctaa   5820 

gaagtagatt ccctttcctc gttgcatttt ttaagacaag gaagtttccc atcagcgaat   5880 

gaacatctgt gactagtgcc gaagcttact aatgctaagg gctggcccag attattaagc   5940 

taataaaaaa tatcgttcag caacagacct gcctcgtctt tgcaaaccca gtgtgcttta   6000 

aaccaatctg caaacatagc agatctgctg atccccaccc tgtgattcag ggggctccga   6060 

ggatacgttt ggtcagagtg attggctcca gaactttgta cagtacaaac ccagggaaaa   6120 

ccattcactc tcctggtatt ccagtcttca gtttataaca cactgtactg gttaggacag   6180 

agtcttagga gtagactaag aagggagaaa tggtgacgtc ccagcagggt catgatgaat   6240 

cagctctggc taactccaca taaaagactt cagtgcattt ctgaagatta attacttcac   6300 

cagggggaca cggtggcctt ggtgttattt gaagagtgtc ctcagctaat tcacaggaca   6360 

gtaaatagct cgtgggtggt gactgtcgaa cgagagagcc tctcgggaat ggcacagagt   6420 

aatcaacaga taccttttaa accgagacca ttctctacca aacatgaaaa tgacaataaa   6480 

gggtccagca aaaaaacaaa caccaaaatc agaagaacat cttcctccta aagttactaa   6540 

cccctggggt ggggggaagc ccacacaaaa tagaaacccc ccaaaaacct ctctccattt   6600 

taatgggtct agacatagtt tctgtcagat tacactgagc cctgctaccc gatatctggc   6660 

aagcctgtag caccagttct catctctcct gtgttaactt taggttcact tgaggatgtg   6720 

ccttttgaag atttttttta tttagtaatt attatttttt tataaaactt gataaaaaat   6780 

agtatttcca actgtacagt cactag                                        6806 

 
           
             106  
             646  
             PRT  
             Rattus norvegicus  
             
               Heat shock cognate protein 70  
             
           
            106 

Met Ser Lys Gly Pro Ala Val Gly Ile Asp Leu Gly Thr Thr Tyr Ser 
1               5                   10                  15 

Cys Val Gly Val Phe Gln His Gly Lys Val Glu Ile Ile Ala Asn Asp 
            20                  25                  30 

Gln Gly Asn Arg Thr Thr Pro Ser Tyr Val Ala Phe Thr Asp Thr Glu 
        35                  40                  45 

Arg Leu Ile Gly Asp Ala Ala Lys Asn Gln Val Ala Met Asn Pro Thr 
    50                  55                  60 

Asn Thr Val Phe Asp Ala Lys Arg Leu Ile Gly Arg Arg Phe Asp Asp 
65                  70                  75                  80 

Ala Val Val Gln Ser Asp Met Lys His Trp Pro Phe Met Val Val Asn 
                85                  90                  95 

Asp Ala Gly Arg Pro Lys Val Gln Val Glu Tyr Lys Gly Glu Thr Lys 
            100                 105                 110 

Ser Phe Tyr Pro Glu Glu Val Ser Ser Met Val Leu Thr Lys Met Lys 
        115                 120                 125 

Glu Ile Ala Glu Ala Tyr Leu Gly Lys Thr Val Thr Asn Ala Val Val 
    130                 135                 140 

Thr Val Pro Ala Tyr Phe Asn Asp Ser Gln Arg Gln Ala Ala Lys Asp 
145                 150                 155                 160 

Ala Gly Thr Ile Ala Gly Leu Asn Val Leu Arg Ile Ile Asn Glu Pro 
                165                 170                 175 

Thr Ala Ala Ala Ile Ala Tyr Gly Leu Asp Lys Lys Val Arg Ala Glu 
            180                 185                 190 

Arg Asn Val Leu Ile Phe Asp Leu Gly Gly Gly Thr Phe Asp Val Ser 
        195                 200                 205 

Ile Leu Thr Thr Glu Asp Gly Ile Phe Glu Val Lys Ser Thr Ala Gly 
    210                 215                 220 

Asp Thr His Leu Gly Gly Glu Asp Phe Asp Asn Arg Met Val Asn His 
225                 230                 235                 240 

Phe Ile Ala Glu Phe Lys Arg Lys His Lys Lys Asp Ile Ser Glu Asn 
                245                 250                 255 

Lys Arg Ala Val Arg Arg Leu Arg Thr Ala Cys Glu Arg Ala Lys Arg 
            260                 265                 270 

Thr Leu Ser Ser Ser Thr Gln Ala Ser Ile Glu Ile Asp Ser Leu Tyr 
        275                 280                 285 

Glu Gly Ile Asp Phe Tyr Thr Ser Ile Thr Arg Ala Arg Phe Glu Glu 
    290                 295                 300 

Leu Asn Ala Asp Leu Phe Arg Gly Thr Leu Asp Pro Val Glu Lys Ala 
305                 310                 315                 320 

Leu Arg Asp Ala Lys Leu Asp Lys Ser Gln Ile His Asp Ile Val Leu 
                325                 330                 335 

Val Gly Gly Ser Thr Arg Ile Pro Lys Ile Gln Lys Leu Leu Gln Asp 
            340                 345                 350 

Phe Phe Asn Gly Lys Glu Leu Asn Lys Ser Ile Asn Pro Asp Glu Ala 
        355                 360                 365 

Val Ala Tyr Gly Ala Ala Val Gln Ala Ala Ile Leu Ser Gly Asp Lys 
    370                 375                 380 

Ser Glu Asn Val Gln Asp Leu Leu Leu Leu Asp Val Thr Pro Leu Ser 
385                 390                 395                 400 

Leu Gly Ile Glu Thr Ala Gly Gly Val Met Thr Val Leu Ile Lys Arg 
                405                 410                 415 

Asn Thr Thr Ile Pro Thr Lys Gln Thr Gln Thr Phe Thr Thr Tyr Ser 
            420                 425                 430 

Asp Asn Gln Pro Gly Val Leu Ile Gln Val Tyr Glu Gly Glu Arg Ala 
        435                 440                 445 

Met Thr Lys Asp Asn Asn Leu Leu Gly Lys Phe Glu Leu Thr Gly Ile 
    450                 455                 460 

Pro Pro Ala Pro Arg Gly Val Pro Gln Ile Glu Val Thr Phe Asp Ile 
465                 470                 475                 480 

Asp Ala Asn Gly Ile Leu Asn Val Ser Ala Val Asp Lys Ser Thr Gly 
                485                 490                 495 

Lys Glu Asn Lys Ile Thr Ile Thr Asn Asp Lys Gly Arg Leu Ser Lys 
            500                 505                 510 

Glu Asp Ile Glu Arg Met Val Gln Glu Ala Glu Lys Tyr Lys Ala Glu 
        515                 520                 525 

Asp Glu Lys Gln Arg Asp Lys Val Ser Ser Lys Asn Ser Leu Glu Ser 
    530                 535                 540 

Tyr Ala Phe Asn Met Lys Ala Thr Val Glu Asp Glu Lys Leu Gln Gly 
545                 550                 555                 560 

Lys Ile Asn Asp Glu Asp Lys Gln Lys Ile Leu Asp Lys Cys Asn Glu 
                565                 570                 575 

Ile Ile Ser Trp Leu Asp Lys Asn Gln Thr Ala Glu Lys Glu Glu Phe 
            580                 585                 590 

Glu His Gln Gln Lys Glu Leu Glu Lys Val Cys Asn Pro Ile Ile Thr 
        595                 600                 605 

Lys Leu Tyr Gln Ser Ala Gly Gly Met Pro Gly Gly Met Pro Gly Gly 
    610                 615                 620 

Phe Pro Gly Gly Gly Ala Pro Pro Ser Gly Gly Ala Ser Ser Gly Pro 
625                 630                 635                 640 

Thr Ile Glu Glu Val Asp 
                645 

 
           
             107  
             2242  
             DNA  
             Rattus norvegicus  
             
               Heat shock cognate protein 70  
             
           
            107 

atgcactttc aaagtagtga gaaaattttg atgttttcta atacatagaa atgatatcat     60 

taaaaggcag atgtggctca ttgaacgcag agccagctct cgggggtctc tgtgtggtct    120 

cgtcatcagc acagctgggc ctacacgcaa gcaaccatgt ctaagggacc tgcagttggc    180 

attgatcttg gcaccaccta ctcctgtgtg ggtgtcttcc agcatggaaa ggtggaaata    240 

attgccaatg accagggtaa ccgcaccacg ccgagctatg ttgctttcac cgacacagaa    300 

cgattaattg gggatgcggc caagaatcag gttgcaatga accccaccaa cacagttttt    360 

gatgccaaac gtctgatcgg acgtaggttc gatgatgctg ttgttcagtc tgacatgaag    420 

cactggccct tcatggtggt gaacgatgca ggcaggccca aggtccaagt cgaatacaaa    480 

ggggagacaa aaagtttcta tcctgaggaa gtgtcttcaa tggttctgac aaaaatgaag    540 

gaaattgcag aagcttacct tggaaagact gttaccaatg ccgtggtcac cgtgccagct    600 

tacttcaatg actctcagcg acaggcagca aaagatgctg gaactattgc tggcctcaac    660 

gtacttcgaa ttatcaatga gccaactgct gctgctattg cctatggctt agataagaag    720 

gtcagggctg aaaggaatgt gctcattttt gacttgggag gtggcacttt tgatgtgtca    780 

attctgacta ctgaggatgg aatttttgaa gtcaaatcaa cagctggaga cacccacttg    840 

ggcggagaag actttgacaa ccgaatggtc aaccatttca ttgctgagtt taagcgaaag    900 

cacaagaagg acatcagtga gaacaagaga gctgtcaggc gtctccgcac tgcctgtgag    960 

cgggccaagc gcaccctctc ctccagcacc caggccagta ttgagattga ttctctctat   1020 

gagggaattg acttctacac ctccattacc cgtgctcgat ttgaggagtt gaatgctgac   1080 

ctgttccgtg gcacactgga ccctgtagag aaggcccttc gagatgccaa actagacaag   1140 

tcacagatcc atgatattgt cctggtgggt ggttctacca gaatccccaa gatccagaaa   1200 

cttctgcaag acttcttcaa tggaaaagag ctgaataaga gcattaaccc cgatgaagct   1260 

gttgcctatg gtgcagctgt ccaggcagcc attctatctg gagacaagtc tgagaatgtt   1320 

caggatttgc tgctcttgga tgtcactcct ctttcccttg ggattgaaac tgctggtgga   1380 

gtcatgactg tcctcatcaa gcgcaatacc accattccca ccaagcagac ccagactttc   1440 

accacctact ctgacaacca gccaggtgta ctcatccagg tgtatgaagg tgaaagggcc   1500 

atgaccaagg acaacaacct gcttgggaag tttgagctca caggcatacc tccagcaccc   1560 

cgtggggttc ctcagattga ggttactttt gacattgatg ccaatggcat cctcaatgtt   1620 

tctgctgtag ataagagcac aggaaaggag aacaagatca ccatcaccaa tgacaagggc   1680 

cgcttgagta aggaggatat tgagcgcatg gtccaagaag ctgagaagta caaagctgag   1740 

gatgagaagc agagagataa ggtttcctct aagaactcgc tggagtctta tgctttcaac   1800 

atgaaagcaa ctgttgagga tgagaaactt caaggcaaga tcaatgatga agacaaacag   1860 

aagattcttg acaagtgcaa cgaaatcatc agctggctgg ataagaacca gactgcggag   1920 

aaggaagaat ttgagcatca gcagaaagaa ctggagaagg tctgcaaccc tatcatcacc   1980 

aagctgtacc agagtgctgg tggcatgcct ggaggaatgc ctggtggctt ccctggtgga   2040 

ggagctcctc catctggtgg tgcttcttca ggccccacca ttgaagaggt cgattaagtc   2100 

aaagtagagg gtgtagcatt gttccacagg gacccaaaac aagtaacatg gaataataaa   2160 

actatttaaa ttggcacaaa aaaaaaaaaa aaaacggaga tgtggtgact cagctgtaat   2220 

cccggcactt aggaagctga gg                                            2242 

 
           
             108  
             374  
             PRT  
             Rattus norvegicus  
             
               Tau microtubule associated protein  
             
           
            108 

Met Ala Glu Pro Arg Gln Glu Phe Asp Thr Met Glu Asp Gln Ala Gly 
1               5                   10                  15 

Asp Tyr Thr Met Leu Gln Asp Gln Glu Gly Asp Met Asp His Gly Leu 
            20                  25                  30 

Lys Ala Glu Glu Ala Gly Ile Gly Asp Thr Pro Asn Met Glu Asp Gln 
        35                  40                  45 

Ala Ala Gly His Val Thr Gln Ala Arg Val Ala Gly Val Ser Lys Asp 
    50                  55                  60 

Arg Thr Gly Asn Asp Glu Lys Lys Ala Lys Gly Ala Asp Gly Lys Thr 
65                  70                  75                  80 

Gly Ala Lys Ile Ala Thr Pro Arg Gly Ala Ala Thr Pro Gly Gln Lys 
                85                  90                  95 

Gly Thr Ser Asn Ala Thr Arg Ile Pro Ala Lys Thr Thr Pro Ser Pro 
            100                 105                 110 

Lys Thr Pro Pro Gly Ser Gly Glu Pro Pro Lys Ser Gly Glu Arg Ser 
        115                 120                 125 

Gly Tyr Ser Ser Pro Gly Ser Pro Gly Thr Pro Gly Ser Arg Ser Arg 
    130                 135                 140 

Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg Glu Pro Lys Lys Val Ala 
145                 150                 155                 160 

Val Val Arg Thr Pro Pro Lys Ser Pro Ser Ala Ser Lys Ser Arg Leu 
                165                 170                 175 

Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Arg Ser Lys 
            180                 185                 190 

Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys Val 
        195                 200                 205 

Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys 
    210                 215                 220 

Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser Val Gln 
225                 230                 235                 240 

Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys Cys Gly 
                245                 250                 255 

Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln Val Glu Val 
            260                 265                 270 

Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser Lys Ile Gly 
        275                 280                 285 

Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn Lys Lys Ile 
    290                 295                 300 

Glu Thr His Lys Leu Thr Phe Arg Glu Asn Ala Lys Ala Lys Thr Asp 
305                 310                 315                 320 

His Gly Ala Glu Ile Val His Lys Ser Pro Val Val Ser Gly Asp Thr 
                325                 330                 335 

Ser Pro Arg His Leu Ser Asn Val Ser Ser Thr Gly Ser Ile Asp Met 
            340                 345                 350 

Val Asp Ser Pro Gln Leu Ala Thr Leu Ala Asp Glu Val Ser Ala Ser 
        355                 360                 365 

Leu Ala Lys Gln Gly Leu 
    370 

 
           
             109  
             5240  
             DNA  
             Rattus norvegicus  
             
               Tau microtubule associated protein  
             
           
            109 

cctcccctgg ggaggttcgc gttcccgtgg ctcgcgcctg ctgcccgccg gcctcgagaa     60 

cgcgctctct cggccgcggc gctcacagtc tccgccaccc accagctcca gcaccagcag    120 

cagcgccggc gccaccgccc accttctgct gtcgccgccg ccacaaccac cttcccctcc    180 

gctgtcctct tctgtcctcg cctcctgtcg attatcaggc tttgaagcag catggctgaa    240 

ccccgccagg agtttgacac aatggaagac caggccggag attacactat gctccaagac    300 

caagaaggag acatggacca tggcttaaaa gctgaagaag caggcatcgg agacaccccg    360 

aacatggagg accaagctgc tgggcatgtg actcaagctc gagtggccgg cgtaagcaaa    420 

gacaggacag gaaatgacga gaagaaagcg aagggggccg atggcaaaac gggggcgaag    480 

atcgccacac ctcggggagc agccactccg ggccagaaag gcacatccaa tgccaccagg    540 

atcccagcca agaccacacc cagcccaaag actcctccag gatcaggtga accaccaaaa    600 

tccggagaac gaagcggcta cagcagcccc ggctcgcccg gaacccctgg cagtcgctcc    660 

cgtaccccat ccctaccaac gccgcccacc cgagagccca aaaaggtggc agtggttcgc    720 

actcccccta agtcaccgtc tgccagtaag agccgcctac agactgcccc tgtgcccatg    780 

ccagacctaa agaacgtcag gtccaagatt ggctccactg agaacctgaa gcaccagccg    840 

ggaggcggca aggtgcagat aattaataag aagctggatc ttagcaacgt ccagtccaag    900 

tgtggctcaa aggacaatat caaacacgtc ccgggcggag gcagtgtgca aatagtctac    960 

aagccagtgg acctgagcaa ggtgacctcc aagtgtggtt ccttagggaa catccatcac   1020 

aagccaggag gtggccaggt agaagtaaaa tcagagaagc tggacttcaa ggatagagtc   1080 

cagtcgaaga ttggctcctt ggataacatc acccatgtcc ctggaggagg gaataagaag   1140 

attgaaaccc acaagctgac cttcagggag aatgccaaag ccaagacaga ccatggagca   1200 

gaaatcgtgc acaagtcacc tgtggtgtct ggggacacat ctccacggca cctcagcaac   1260 

gtctcctcca cgggcagcat cgacatggtg gactctccac agcttgccac gttagccgat   1320 

gaagtgtccg cctctttggc caagcagggt ttgtgatcag gcccctgggg ccgtcactga   1380 

tcatggagag aagagagagt gagagtgtgg aaaaaaaaaa aaaaagaatg acctggcccc   1440 

tcaccctctg ccctccccgc tgctcctcat agacaggctg accagcttgt cacctaacct   1500 

gcttttgtgg ctcgggtttg gctcgggact tcaaaatcag tgatgggaaa aagtaaattt   1560 

catctttcca aattgatttg tgggctagta ataaaatatt tttaaggaag gaaaaaaaaa   1620 

acacgtaaaa ccatggccaa acaaaaccca acatttcctt ggcaattgtt attgaccccg   1680 

cccccccctc tgagttttag agggtgaagg aggctttgga tagaggctgc ttctggggat   1740 

tggctgaggg actagggcaa ctaattgccc acagccccat cttaggggca tcagggacag   1800 

cggcagacat gaaagacttg ggacttggtg tgtttgtgga gccgtaaggc gtatgttaac   1860 

tttgtgtggg tttgagggag gactgtgata gtgaaggctg agagatgggt gggctgggag   1920 

tcagaggaga gaggtgagga agacaggttg ggagaggggg cattgcgtcc ttgccaagga   1980 

gcttgggaag cacaggtagc cctggctgca gcagtcttag ctagcacaga tgcctgcctg   2040 

agaaagcaca gtggggtaca gtgggtgtgt gtgccccttc tgaagggcag cccatgggag   2100 

aaggggtatt gggcagaagg aaggtaggcc ccagaaggtg gcaccttgta gattggttct   2160 

ctgaaggctg accttgccat cccagggcac tgctcccacc ctccaggagg aggtctgagc   2220 

tgaggagctt ccttttcgat ctcacaggaa aacctgtgtt actgagttct gaagtttgga   2280 

actacagcca tgattttggc caccatacag acctgggact ttagggctaa ccagttcttt   2340 

gtaaggactt gtgcctcttg cgggaacatc tgcctgttct caagcctggt cctctggcac   2400 

ttctgcagtg gtgagggatg ggggtggtat tctgggatgt gggtcccagg cctcccatcc   2460 

ctcgcacagc cactgtatcc cctctacctg tcctatcatg cccacgtctg ccacgagagc   2520 

cagtcactgc cgtccgtaca tcacgtctca ccgtcctgag tgcccagcct cccaagccca   2580 

atccctggac ccctgggtag ttatggccaa tctgctctac actaggggtt ggagtccagg   2640 

gaaggcaaag atttgggcct tggtctctag tcctacgttg ccagaatcca accagtgtgc   2700 

ctcccacaag gaaccttaca accttgtttg gtttgctcca tcaggcgttt ggcgccatcg   2760 

tggatggagt ccgtgtgtgc ctggagatta ccctggacac ctctgctttt ttttttttta   2820 

ctttagcggt tgcctcctag gcctgactcc ttcccatgtt gaactggagg cagccaagtt   2880 

aggtgtcaat gtcctggcat cagtatgaac agtcagtagt cccagggcag ggccacactt   2940 

ctcccatctt ctgcttccac cccagcttgt gattgctagc ctcccagagc tcagccgcca   3000 

ttaagtcccc atgcacgtaa tcagcccttc cataccccaa tttggggaac ataccccttg   3060 

attgaaatgt tttccctcca gtcctatgga agcggtgctg cctgcctgct ggagcagcca   3120 

gccatctcag agacgcagcc ctttctctcc tgtccgcacc ctgctgcgct gtagtcggat   3180 

tcgtctgttt gtctgggttc accagagtga ctatgatagt gaaaagaaaa agaaaaagaa   3240 

aaaagaaaaa agaaaaaaaa aaaaggacgc atgttatctt gaaatatttg tcaaaaggtt   3300 

gtagcccacc gcagggattg gagggcctga tattccttgt cttcttcgtg acttaggtcc   3360 

aggccggtcg agtgctaccc tgctggacat cccatgtttt gaagggtttc ttcttcatct   3420 

gggacccctg cagacactgg attgtgacat tggaggtcta tacattggcc aaggctgaag   3480 

cacaggaccc gttagaggca gcaggctccg actgtcaggg agagcttgtg gctggcctgt   3540 

ttctctgagt gaagatggtc ctctctaatc acaacttcaa gtcccacagc agccctggca   3600 

gacatctaag aactcctgca tcacaagaga aaaggacact agtaccagca gggagagctg   3660 

tggccctaga aattccatga ctctccacta ctatccgtgg gtcctttcca agccttgcct   3720 

cgtcaccaag ggcttgggat ggactgcccc actgatgaaa gggacatctt tggagacccc   3780 

cttggtttcc aaggcgtcag ccccctgacc ttgcatgacc tcctacagct gaaggatgag   3840 

gcctttaaag attaggaacc tcaggcccag gtcggccact ttgggcttgg gtacagttag   3900 

ggacgatgcg gtagaaggag gtggccaacc tttccatata agagttctgt gtgcccagag   3960 

ctaccctatt gtgagctccc cactgctgat ggactttagc tgtccttaga agtgaagagt   4020 

ccaacggagg aaaaggaagt gtggtttgat ggtctgtggt cccttcatca tggttacctg   4080 

ttgtggtttt ctctgtatac ccccatttac ccatcctgca gttcctgtcc ttgaataggg   4140 

gtgggggtac tctgccatat ctcttgtagg cagtcagccc ccaagtcata gtttggagtg   4200 

atctggtcag tgctaatagg cagtttacaa ggaattctgg cttgttactt cagtgaggac   4260 

aatcccccaa ggccctggca cctgtcctgt ctttccatgg ctctccactg cagagccaat   4320 

gtctttgggt gggctagata gggtgtacaa tttgcctgga acctccaagc tcttaatcca   4380 

ctttatcaat agttccattt aaattgactt caatataaga gtgtatccat ttgagattgc   4440 

ttgtgttgtg gggtaaaggg gggaggagga acatgttaag ataattgaca tgggcaaggg   4500 

gaagtcttga agtgtagcag ttaaaccatc ttgtagcccc attcatgatg ttgaccactt   4560 

gctagagaga agaggtgcca taaggctaga acctagaggc ttggctgtcc accaacaggc   4620 

aggcttttgc aaggcagagg cagccagcta ggtccctgac ttcccagcca ggtgcagctc   4680 

taagaactgc tcttgcctgc tgccttcttg tggtgtccag agcccacagc caatgcctcc   4740 

tcaaaaccct ggcttccttc cttctaatcc actggcacat cagcatcacc tccggattga   4800 

cttcagatcc acagcctaca ctactagcag tgggtaagac cacttccttt gtccttgtct   4860 

gttctccaga aaagtgggca tggaggcggt gttaataact ataggtctgt ggctttatga   4920 

gccttcaaac ttctctctag cttctgaaag ggttactttt gggcagtatt gcagtctcac   4980 

cctccgatgg ctgtagcctg tgcagttgct gtactgggca tgatctccag tgcttgcaag   5040 

tcccatgatt tctttggtgt tttgagggtg gggggaggga catgaatcat cttagcttag   5100 

cttcctgtct gtgaatgtcc atatagtgta ctgtgtttta acaaacgatt tacactgact   5160 

gttgctgtac aagtgaattt ggaaataaag ttattactct gattaaacaa aaaaaaaaaa   5220 

aaaaaaaaaa aaaaaaaaaa                                               5240 

 
           
             110  
             248  
             PRT  
             Rattus norvegicus  
             
               Schwann cell peripheral myelin  
             
           
            110 

Met Ala Pro Gly Ala Pro Ser Ser Ser Pro Ser Pro Ile Leu Ala Ala 
1               5                   10                  15 

Leu Leu Phe Ser Ser Leu Val Leu Ser Pro Thr Leu Ala Ile Val Val 
            20                  25                  30 

Tyr Thr Asp Arg Glu Val Tyr Gly Ala Val Arg Ser Gln Val Thr Leu 
        35                  40                  45 

His Cys Ser Phe Trp Ser Ser Glu Trp Val Ser Asp Asp Ile Ser Phe 
    50                  55                  60 

Thr Trp Arg Tyr Gln Pro Glu Gly Gly Arg Asp Ala Ile Ser Ile Phe 
65                  70                  75                  80 

His Tyr Ala Lys Gly Gln Pro Tyr Ile Asp Glu Val Gly Thr Phe Lys 
                85                  90                  95 

Glu Arg Ile Gln Trp Val Gly Asp Pro Ser Trp Lys Asp Gly Ser Ile 
            100                 105                 110 

Val Ile His Asn Leu Asp Tyr Ser Asp Asn Gly Thr Phe Thr Cys Asp 
        115                 120                 125 

Val Lys Asn Pro Pro Asp Ile Val Gly Lys Thr Ser Gln Val Thr Leu 
    130                 135                 140 

Tyr Val Phe Glu Lys Val Pro Thr Arg Tyr Gly Val Val Leu Gly Ala 
145                 150                 155                 160 

Val Ile Gly Gly Ile Leu Gly Val Val Leu Leu Leu Leu Leu Leu Phe 
                165                 170                 175 

Tyr Leu Ile Arg Tyr Cys Trp Leu Arg Arg Gln Ala Ala Leu Gln Arg 
            180                 185                 190 

Arg Leu Ser Ala Met Glu Lys Gly Lys Phe His Lys Ser Ser Lys Asp 
        195                 200                 205 

Ser Ser Lys Arg Gly Arg Gln Thr Pro Val Leu Tyr Ala Met Leu Asp 
    210                 215                 220 

His Ser Arg Ser Thr Lys Ala Ala Ser Glu Lys Lys Ser Lys Gly Leu 
225                 230                 235                 240 

Gly Glu Ser Arg Lys Asp Lys Lys 
                245 

 
           
             111  
             1029  
             DNA  
             Rattus norvegicus  
             
               Schwann cell peripheral myelin  
             
           
            111 

attcggctgg gcccttgccc ctaccccagc tatggctcct ggggctccct catccagccc     60 

cagccctatc ctggctgccc tgctcttctc ttctttggtg ctgtccccaa ccctggccat    120 

tgtggtttac acggacaggg aagtctatgg tgctgtgcgc tcccaggtca ccctgcactg    180 

ctccttctgg tccagtgaat gggtctcaga tgacatctct tttacctggc gctaccagcc    240 

tgaaggaggc cgagatgcca tttcaatctt ccactatgcc aagggtcaac cttacatcga    300 

tgaggtgggg accttcaagg agcgcatcca gtgggtaggg gaccctagct ggaaggatgg    360 

ctccattgtc atacacaacc tagactacag tgacaacggc actttcacat gtgatgtcaa    420 

aaacccaccg gacatagtgg gcaagacgtc tcaggtcacg ctctatgtct ttgaaaaagt    480 

gcccactagg tatggggtgg tgttgggagc cgtgatcggt ggcatcctcg gggtggtgct    540 

gttgctgctg ttgctcttct acctgatccg gtactgctgg ctgcgcaggc aggctgccct    600 

gcagaggagg ctcagtgcca tggagaaggg gaaatttcac aagtcttcta aggactcctc    660 

gaagcgcggg cggcagacgc cagtgctgta tgccatgctg gaccacagcc gaagcaccaa    720 

agctgccagt gagaagaaat ctaaagggct gggggagtct cgcaaggata agaaatagcg    780 

gttagcgggc cgggcggggg gtcgggggtc tgcgatggag tcttccaaag gctctcaggt    840 

ggtggtcatc gagatggagc ttcgcaaaga tgagcagagc tcggagcccc ggcctgcagt    900 

caaatccccc agtagaacca gcctcaagaa cgccctcaag aacatgatgg gcctggactc    960 

gaacaagtga ccgtcacctc caggctctgt cagagcaggg gacctaggct cctctttttc   1020 

ccagtctag                                                           1029 

 
           
             112  
             444  
             PRT  
             Rattus norvegicus  
             
               Class 1 beta tubulin  
             
           
            112 

Met Arg Glu Ile Val His Ile Gln Ala Gly Gln Cys Gly Asn Gln Ile 
1               5                   10                  15 

Gly Ala Lys Phe Trp Glu Val Ile Ser Asp Glu His Gly Ile Asp Pro 
            20                  25                  30 

Thr Gly Thr Tyr His Gly Asp Ser Asp Leu Gln Leu Asp Arg Ile Ser 
        35                  40                  45 

Val Tyr Tyr Asn Glu Ala Thr Gly Gly Lys Tyr Val Pro Arg Ala Ile 
    50                  55                  60 

Leu Val Asp Leu Glu Pro Gly Thr Met Asp Ser Val Arg Ser Gly Pro 
65                  70                  75                  80 

Phe Gly Gln Ile Phe Arg Pro Asp Asn Phe Val Phe Gly Gln Ser Gly 
                85                  90                  95 

Ala Gly Asn Asn Trp Ala Lys Gly His Tyr Thr Glu Gly Ala Glu Leu 
            100                 105                 110 

Val Asp Ser Val Leu Asp Val Val Arg Lys Glu Ala Glu Ser Cys Asp 
        115                 120                 125 

Cys Leu Gln Gly Phe Gln Leu Thr His Ser Leu Gly Gly Gly Thr Gly 
    130                 135                 140 

Ser Gly Met Gly Thr Leu Leu Ile Ser Lys Ile Arg Glu Glu Tyr Pro 
145                 150                 155                 160 

Asp Arg Ile Met Asn Thr Phe Ser Val Val Pro Ser Pro Lys Val Ser 
                165                 170                 175 

Asp Thr Val Val Glu Pro Tyr Asn Ala Thr Leu Ser Val His Gln Leu 
            180                 185                 190 

Val Glu Asn Thr Asp Glu Thr Tyr Cys Ile Asp Asn Glu Ala Leu Tyr 
        195                 200                 205 

Asp Ile Cys Phe Arg Thr Leu Lys Leu Thr Thr Pro Thr Tyr Gly Asp 
    210                 215                 220 

Leu Asn His Leu Val Ser Ala Thr Met Ser Gly Val Thr Thr Cys Leu 
225                 230                 235                 240 

Arg Phe Pro Gly Gln Leu Asn Ala Asp Leu Arg Lys Leu Ala Val Asn 
                245                 250                 255 

Met Val Pro Phe Pro Arg Leu His Phe Phe Met Pro Gly Phe Ala Pro 
            260                 265                 270 

Leu Thr Ser Arg Gly Ser Gln Gln Tyr Arg Ala Leu Thr Val Pro Glu 
        275                 280                 285 

Leu Thr Gln Gln Val Phe Asp Ala Lys Asn Met Met Ala Ala Cys Asp 
    290                 295                 300 

Pro Arg His Gly Arg Tyr Leu Thr Val Ala Ala Val Phe Arg Gly Arg 
305                 310                 315                 320 

Met Ser Met Lys Glu Val Asp Glu Gln Met Leu Asn Val Gln Asn Lys 
                325                 330                 335 

Asn Ser Ser Tyr Phe Val Glu Trp Ile Pro Asn Asn Val Lys Thr Ala 
            340                 345                 350 

Val Cys Asp Ile Pro Pro Arg Gly Leu Lys Met Ala Val Thr Phe Ile 
        355                 360                 365 

Gly Asn Ser Thr Ala Ile Gln Glu Leu Phe Lys Arg Ile Ser Glu Gln 
    370                 375                 380 

Phe Thr Ala Met Phe Arg Arg Lys Ala Phe Leu His Trp Tyr Thr Gly 
385                 390                 395                 400 

Glu Gly Met Asp Glu Met Glu Phe Thr Glu Ala Glu Ser Asn Met Asn 
                405                 410                 415 

Asp Leu Val Ser Glu Tyr Gln Gln Tyr Gln Asp Ala Thr Ala Glu Glu 
            420                 425                 430 

Glu Glu Asp Phe Gly Glu Glu Ala Glu Glu Glu Ala 
        435                 440 

 
           
             113  
             2346  
             DNA  
             Rattus norvegicus  
             
               Class 1 beta tubulin  
             
           
            113 

ctcgcatctc tctgcagcag cctccggggc tacgttccat cctatcagtc tgagaccagc     60 

ccagaagaaa aaaaccaaaa atccttaatt atcttctttt gctcggtacc tacattggga    120 

ccatcaaaaa aaaattatta cagtaaaccg tagccatgag ggaaatcgtg cacatccagg    180 

ccggacagtg tggcaaccag atcggtgcta agttctggga ggtgataagc gatgaacacg    240 

gcatcgaccc caccggcacc taccacggag acagcgactt gcagctggac cgaatctctg    300 

tgtactacaa tgaagctaca ggtggcaagt atgtccctcg agctatctta gtggatctag    360 

aacccgggac tatggactcc gttcgctcag gtccttttgg ccagatcttc agaccggaca    420 

actttgtttt tggtcagtct ggggcaggca acaactgggc taagggtcac tacacagagg    480 

gagctgagct ggttgactct gtcttggatg tggtgcggaa ggaggcggag agctgtgact    540 

gcctgcaagg ctttcagctg acccactcgc tgggtggagg cacgggctct ggcatgggca    600 

ccctgctcat cagcaagatt cgagaagaat accccgaccg catcatgaac accttcagcg    660 

tggtgccctc acccaaagtg tctgacaccg tggttgagcc ctacaacgcc accctgtccg    720 

tccaccagtt ggtagagaac acagatgaga cctactgcat tgacaacgag gctctctacg    780 

acatctgctt ccgtaccctt aagctcacca cgccaaccta cggagacctg aaccatctcg    840 

tctctgccac catgagcggt gtcaccacct gcctccgttt cccgggccag ctgaacgccg    900 

accttcggaa gctggctgtc aacatggtgc ccttcccgcg tctccacttc ttcatgcctg    960 

gctttgcccc tctcaccagc cgtggaagcc agcagtaccg ggccctcact gtgcctgaac   1020 

tcacccagca ggtcttcgac gccaagaaca tgatggccgc ctgtgaccca cgccacggcc   1080 

ggtacctcac ggttgcggct gtcttccgtg gacggatgtc catgaaggag gtagatgagc   1140 

aaatgctcaa cgtgcagaat aagaatagca gctacttcgt ggaatggatc cccaacaacg   1200 

tcaagacggc tgtctgtgac atcccaccgc gaggcctcaa gatggcagtc accttcatcg   1260 

gaaacagcac ggccatccag gagctcttca agcgcatctc tgagcagttt acggcaatgt   1320 

tccgtcggaa ggctttcctc cactggtaca cgggtgaggg catggacgag atggagttca   1380 

ccgaagctga gagcaacatg aatgacctcg tctctgagta tcagcagtac caggacgcca   1440 

ccgcagaaga ggaagaggat ttcggagagg aggcagaaga agaggcctaa ggcggaagcc   1500 

ctgcgtcacc tcaggctgct tagttccctt agcttttttc caactgccct ttgtttcttt   1560 

ctgctgcctc tgtcctgttt ttgttccgtt ttgttttctc attggggggt aaacggtgcc   1620 

tggcacgtgg caggcgctca ataaatattt ctttgtggaa tgtctccttt ctctttccga   1680 

tctgacattc tgagcgttaa ccctgtgctt cctcctggta cccatttctt tattatgact   1740 

gtccagttaa tattcttcca atatattctc caggaaccag cgttcggctc cagacccggg   1800 

gtagaaccca gcccagttct aagaactcaa gagagtagcc accatcttaa agcaaagtag   1860 

cgggtgggga aaaggtgggt agaggagatg ctacttaggg ggtgggattt tccagtcagg   1920 

gccatttaga cacgggaatc gctggggctt tcagtcgatg gggctttctt ttttccctgc   1980 

ttcatctctc ggcctcagga gaggtattaa cagtattatc accatttata tcctagctgt   2040 

cctgagccaa atctgccatt ggaggtgtct tcccctgtgt tggttctcca agggacgttg   2100 

tatggggatt tgtgggggca gggcagcaag gccttgttct ctaaatgtcc agaaacactc   2160 

taattaccat ttccacctgg gtgcctcaca cactccccag aggggaggta acatctctgc   2220 

cccatttcca tcctgttcct tggcttggtc attccctacc tttctatttt ctgttaaact   2280 

tggctttttt tttttcatat tgaaaagatg acattgcccc gagagccaaa aataaatggg   2340 

aattgg                                                              2346 

 
           
             114  
             724  
             PRT  
             Rattus norvegicus  
             
               Heat shock protein 90  
             
           
            114 

Met Pro Glu Glu Val His His Gly Glu Glu Glu Val Glu Thr Phe Ala 
1               5                   10                  15 

Phe Gln Ala Glu Ile Ala Gln Leu Met Ser Leu Ile Ile Asn Thr Phe 
            20                  25                  30 

Tyr Ser Asn Lys Glu Ile Phe Leu Arg Glu Leu Ile Ser Asn Ala Ser 
        35                  40                  45 

Asp Ala Leu Asp Lys Ile Arg Tyr Glu Ser Leu Thr Asp Pro Ser Lys 
    50                  55                  60 

Leu Asp Ser Gly Lys Glu Leu Lys Ile Asp Ile Ile Pro Asn Pro Gln 
65                  70                  75                  80 

Glu Ala Thr Leu Thr Leu Val Asp Thr Gly Ile Gly Met Thr Lys Ala 
                85                  90                  95 

Asp Leu Ile Asn Asn Leu Gly Thr Ile Ala Lys Ser Gly Thr Lys Ala 
            100                 105                 110 

Phe Met Glu Ala Leu Gln Ala Gly Ala Asp Ile Ser Met Ile Gly Gln 
        115                 120                 125 

Phe Gly Val Gly Phe Tyr Ser Ala Tyr Leu Val Ala Glu Lys Val Val 
    130                 135                 140 

Val Ile Thr Lys His Asn Asp Asp Glu Gln Tyr Ala Trp Glu Ser Ser 
145                 150                 155                 160 

Ala Gly Gly Ser Phe Thr Val Arg Ala Asp His Gly Glu Pro Ile Gly 
                165                 170                 175 

Arg Gly Thr Lys Val Ile Leu His Leu Lys Glu Asp Gln Thr Glu Tyr 
            180                 185                 190 

Leu Glu Glu Arg Arg Val Lys Glu Val Val Lys Lys His Ser Gln Phe 
        195                 200                 205 

Ile Gly Tyr Pro Ile Thr Leu Tyr Leu Glu Lys Glu Arg Glu Lys Glu 
    210                 215                 220 

Ile Ser Asp Asp Glu Ala Glu Glu Glu Lys Gly Glu Lys Glu Glu Glu 
225                 230                 235                 240 

Asp Lys Glu Asp Glu Glu Lys Pro Lys Ile Glu Asp Val Gly Ser Asp 
                245                 250                 255 

Glu Glu Asp Asp Ser Gly Lys Asp Lys Lys Lys Lys Thr Lys Lys Ile 
            260                 265                 270 

Lys Glu Lys Tyr Ile Asp Gln Glu Glu Leu Asn Lys Thr Lys Pro Ile 
        275                 280                 285 

Trp Thr Arg Asn Pro Asp Asp Ile Thr Gln Glu Glu Tyr Gly Glu Phe 
    290                 295                 300 

Tyr Lys Ser Leu Thr Asn Asp Trp Glu Asp His Leu Ala Val Lys His 
305                 310                 315                 320 

Phe Ser Val Glu Gly Gln Leu Glu Phe Arg Ala Leu Leu Phe Ile Pro 
                325                 330                 335 

Arg Arg Ala Pro Phe Asp Leu Phe Glu Asn Lys Lys Lys Lys Asn Asn 
            340                 345                 350 

Ile Lys Leu Tyr Val Arg Arg Val Phe Ile Met Asp Ser Cys Asp Asp 
        355                 360                 365 

Leu Ile Pro Glu Tyr Leu Asn Phe Ile Arg Gly Val Val Asp Ser Glu 
    370                 375                 380 

Asp Leu Pro Leu Asn Ile Ser Arg Glu Met Leu Gln Gln Ser Lys Ile 
385                 390                 395                 400 

Leu Lys Val Ile Arg Lys Asn Ile Val Lys Lys Cys Leu Glu Leu Phe 
                405                 410                 415 

Ser Glu Leu Ala Glu Asp Lys Glu Asn Tyr Lys Lys Phe Tyr Glu Ala 
            420                 425                 430 

Phe Ser Lys Asn Leu Lys Leu Gly Ile His Glu Asp Ser Thr Asn Arg 
        435                 440                 445 

Arg Arg Leu Ser Glu Leu Leu Arg Tyr His Thr Ser Gln Ser Gly Asp 
    450                 455                 460 

Glu Met Thr Ser Leu Ser Glu Tyr Val Ser Arg Met Lys Glu Thr Gln 
465                 470                 475                 480 

Lys Ser Ile Tyr Tyr Ile Thr Gly Glu Ser Lys Glu Gln Val Ala Asn 
                485                 490                 495 

Ser Ala Phe Val Glu Arg Val Arg Lys Arg Gly Phe Glu Val Val Tyr 
            500                 505                 510 

Met Thr Glu Pro Ile Asp Glu Tyr Cys Val Gln Gln Leu Lys Glu Phe 
        515                 520                 525 

Asp Gly Lys Ser Leu Val Ser Val Thr Lys Glu Gly Leu Glu Leu Pro 
    530                 535                 540 

Glu Asp Glu Glu Glu Lys Lys Lys Met Glu Glu Ser Lys Ala Arg Phe 
545                 550                 555                 560 

Glu Asn Leu Cys Lys Leu Met Lys Glu Ile Leu Asp Lys Lys Val Glu 
                565                 570                 575 

Lys Val Thr Ile Ser Asn Arg Leu Val Ser Ser Pro Cys Cys Ile Val 
            580                 585                 590 

Thr Ser Thr Tyr Gly Trp Thr Ala Asn Met Glu Arg Ile Met Lys Ala 
        595                 600                 605 

Gln Ala Leu Arg Asp Asn Ser Thr Met Gly Tyr Met Met Ala Lys Lys 
    610                 615                 620 

His Leu Glu Ile Asn Pro Asp His Pro Ile Val Glu Thr Leu Arg Gln 
625                 630                 635                 640 

Lys Ala Glu Ala Asp Lys Asn Asp Lys Ala Val Lys Asp Leu Val Val 
                645                 650                 655 

Leu Leu Phe Glu Thr Ala Leu Ser Ser Leu Ala Ser His Phe Arg Arg 
            660                 665                 670 

Pro Lys Thr His Ser Asn Arg Ile Tyr Arg Met Ile Lys Leu Gly Leu 
        675                 680                 685 

Gly Ile Asp Glu Asp Glu Val Thr Ala Glu Glu Pro Ser Ala Ala Val 
    690                 695                 700 

Pro Asp Glu Ile Pro Pro Leu Glu Gly Asp Glu Asp Ala Ser Arg Met 
705                 710                 715                 720 

Glu Glu Val Asp 

 
           
             115  
             2524  
             DNA  
             Rattus norvegicus  
             
               Heat shock protein 90  
             
           
            115 

ggcctaggct tgccgtgcga gtcggacgtg gtccgggccc accctgctct gtactactac     60 

tcggctttct cgtcaagatg cctgaggaag tgcaccatgg agaggaagag gtggagacct    120 

tcgcctttca ggcagaaatt gcccagctga tgtccctcat catcaacact ttttactcaa    180 

acaaagagat tttcctccgg gagttgatct ctaatgcttc tgatgccctg gacaagattc    240 

ggtatgagag cctgactgac ccttccaagt tggacagcgg gaaagagctg aagattgaca    300 

tcatccccaa ccctcaggaa gccacgctga ctttggtgga cacaggcatt ggcatgacca    360 

aggctgacct cataaataac ttgggaacca ttgctaagtc tggtacaaag gcgttcatgg    420 

aggctctcca ggctggtgca gacatctcca tgattgggca gtttggtgtc ggcttctact    480 

ctgcctacct ggtggcagag aaagtggttg tgatcacgaa gcacaacgat gatgagcagt    540 

atgcctggga gtcttctgct ggtggatcct tcactgtccg tgcagaccac ggtgagccca    600 

ttggccgggg taccaaagtg atccttcacc tcaaagaaga ccagacagag tacttagagg    660 

agaggagggt caaggaggtg gtgaagaaac actcacagtt cataggctac cccatcaccc    720 

tctatctgga gaaggaacgc gagaaggaga tcagtgatga tgaggcagag gaagagaaag    780 

gggagaagga ggaggaagat aaggaggatg aggagaagcc taagattgag gatgtgggat    840 

ctgatgagga ggatgacagc ggcaaagata agaaaaagaa aacaaagaag atcaaggaga    900 

agtacattga ccaggaagag ctgaataaga cgaagcccat ctggaccaga aatcctgatg    960 

acatcactca agaggaatat ggtgaattct acaagagcct caccaatgac tgggaagacc   1020 

acttggcagt caagcacttc tctgtagaag ggcagttgga attcagggca ttgctcttca   1080 

ttccccggcg agctcccttt gacctctttg agaacaagaa gaagaagaac aacatcaaat   1140 

tgtatgtccg tcgtgtgttc atcatggaca gctgtgatga cctgatacct gagtacctca   1200 

acttcatccg tggtgtggtt gattccgagg acctgcctct gaacatctcc cgagaaatgc   1260 

tccagcagag caagatcctg aaagtcatcc gcaaaaacat tgtgaagaag tgccttgagc   1320 

tcttctctga gttggctgaa gacaaagaga actacaagaa attttatgag gcattctcta   1380 

agaatttaaa gcttggaatc catgaggatt ccactaaccg tcgccgcctc tctgagctcc   1440 

ttcgctacca tacctctcag tctggagatg agatgacttc cctgtcagag tatgtgtctc   1500 

gcatgaagga gacacagaag tccatctact atatcactgg tgagagcaaa gagcaggtgg   1560 

ccaactctgc cttcgtggag cgtgtgcgga aacggggctt tgaggtggta tacatgactg   1620 

agcccattga tgagtactgc gtacagcagc tcaaggagtt cgatggcaag agcctggtct   1680 

ccgtgaccaa ggagggcctg gagctaccag aggatgagga agagaagaaa aaaatggagg   1740 

agagcaaggc aaggtttgag aatctctgca agctcatgaa ggagatcttg gacaagaagg   1800 

ttgaaaaagt gactatctcc aataggcttg tgtcttcccc ctgctgcatt gtgaccagca   1860 

cctacggctg gacagccaac atggaacgga ttatgaaggc ccaggcactg cgggacaact   1920 

cgacaatggg ctacatgatg gccaagaaac acctagagat caaccctgac caccccattg   1980 

tggagactct gcggcagaag gctgaggcag acaaaaacga caaagctgtc aaagacctgg   2040 

tggtgctgct gtttgagact gctctgtctt ctctcgcttc tcactttagg aggcccaaaa   2100 

cccactccaa ccgcatctac cgcatgatta aactaggcct gggcattgat gaagatgagg   2160 

tcactgcaga agagcccagt gctgctgttc ccgatgagat ccccccactg gagggtgatg   2220 

aggatgcctc tcgcatggaa gaagtggatt aaaggctcct gggaagcccc cgccctctgt   2280 

atagtatccc tgtggctccc ccaacagcct tgactggccc tgacccacct gggctctctc   2340 

tgctcatgtc tacaagaatc tcttctatcc tgtccttgtg ccttaaggca ggaagatccc   2400 

ctcccacaga tagcagggtt gggtgttgtg tattgtgttt ttttgtttgt tttattttgt   2460 

tctgaaatta aaagtatgca aaataaagat gatgcagttt taaaaaaaaa aaaaaaaaaa   2520 

aagg                                                                2524 

 
           
             116  
             541  
             PRT  
             Homo sapiens  
             
               Putative ribonuclease III  
             
           
            116 

Met Arg Arg Glu Val Thr Val Glu Leu Ser Ser Gln Gly Phe Trp Lys 
1               5                   10                  15 

Thr Gly Ile Arg Ser Asp Val Cys Gln His Ala Met Met Leu Pro Val 
           20                  25                  30 

Leu Thr His His Ile Arg Tyr His Gln Cys Leu Met His Leu Asp Lys 
       35                  40                  45 

Leu Ile Gly Tyr Thr Phe Gln Asp Arg Cys Leu Leu Gln Leu Ala Met 
   50                  55                  60 

Thr His Pro Ser His His Leu Asn Phe Gly Met Asn Pro Asp His Ala 
65                  70                  75                  80 

Arg Asn Ser Leu Ser Asn Cys Gly Ile Arg Gln Pro Lys Tyr Gly Asp 
                85                  90                  95 

Arg Lys Val His His Met His Met Arg Lys Lys Gly Ile Asn Thr Leu 
            100                 105                 110 

Ile Asn Ile Met Ser Arg Leu Gly Gln Asp Asp Pro Thr Pro Ser Arg 
        115                 120                 125 

Ile Asn His Asn Glu Arg Leu Glu Phe Leu Gly Asp Ala Val Val Glu 
    130                 135                 140 

Phe Leu Thr Ser Val His Leu Tyr Tyr Leu Phe Pro Ser Leu Glu Glu 
145                 150                 155                 160 

Gly Gly Leu Ala Thr Tyr Arg Thr Ala Ile Val Gln Asn Gln His Leu 
                165                 170                 175 

Ala Met Leu Ala Lys Lys Leu Glu Leu Asp Arg Phe Met Leu Tyr Ala 
            180                 185                 190 

His Gly Pro Asp Leu Cys Arg Glu Ser Asp Leu Arg His Ala Met Ala 
        195                 200                 205 

Asn Cys Phe Glu Ala Leu Ile Gly Ala Val Tyr Leu Glu Gly Ser Leu 
    210                 215                 220 

Glu Glu Ala Lys Gln Leu Phe Gly Arg Leu Leu Phe Asn Asp Pro Asp 
225                 230                 235                 240 

Leu Arg Glu Val Trp Leu Asn Tyr Pro Leu His Pro Leu Gln Leu Gln 
                245                 250                 255 

Glu Pro Asn Thr Asp Arg Gln Leu Ile Glu Thr Ser Pro Val Leu Gln 
            260                 265                 270 

Lys Leu Thr Glu Phe Glu Glu Ala Ile Gly Val Ile Phe Thr His Val 
        275                 280                 285 

Arg Leu Leu Ala Arg Ala Phe Thr Leu Arg Thr Val Gly Phe Asn His 
    290                 295                 300 

Leu Thr Leu Gly His Asn Gln Arg Met Glu Phe Leu Gly Asp Ser Ile 
305                 310                 315                 320 

Met Gln Leu Val Ala Thr Glu Tyr Leu Phe Ile His Phe Pro Asp His 
                325                 330                 335 

His Glu Gly His Leu Thr Leu Leu Arg Ser Ser Leu Val Asn Asn Arg 
            340                 345                 350 

Thr Gln Ala Lys Val Ala Glu Glu Leu Gly Met Gln Glu Tyr Ala Ile 
        355                 360                 365 

Thr Asn Asp Lys Thr Lys Arg Pro Val Ala Leu Arg Thr Lys Thr Leu 
    370                 375                 380 

Ala Asp Leu Leu Glu Ser Phe Ile Ala Ala Leu Tyr Ile Asp Lys Asp 
385                 390                 395                 400 

Leu Glu Tyr Val His Thr Phe Met Asn Val Cys Phe Phe Pro Arg Leu 
                405                 410                 415 

Lys Glu Phe Ile Leu Asn Gln Asp Trp Asn Asp Pro Lys Ser Gln Leu 
            420                 425                 430 

Gln Gln Cys Cys Leu Thr Leu Arg Thr Glu Gly Lys Glu Pro Asp Ile 
        435                 440                 445 

Pro Leu Tyr Lys Thr Leu Gln Thr Val Gly Pro Ser His Ala Arg Thr 
    450                 455                 460 

Tyr Thr Val Ala Val Tyr Phe Lys Gly Glu Arg Ile Gly Cys Gly Lys 
465                 470                 475                 480 

Gly Pro Ser Ile Gln Gln Ala Glu Met Gly Ala Ala Met Asp Ala Leu 
                485                 490                 495 

Glu Lys Tyr Asn Phe Pro Gln Met Ala His Gln Lys Arg Phe Ile Glu 
            500                 505                 510 

Arg Lys Tyr Arg Gln Glu Leu Lys Glu Met Arg Trp Glu Arg Glu His 
        515                 520                 525 

Gln Glu Arg Glu Pro Asp Glu Thr Glu Asp Ile Lys Lys 
    530                 535                 540 

 
           
             117  
             1626  
             DNA  
             Homo sapiens  
             
               Putative ribonuclease III  
             
           
            117 

atgagacgag aagtaacggt ggagctaagt agccaaggat tctggaaaac tggcatccgt     60 

tctgatgtct gtcagcatgc aatgatgcta cctgttctga cccatcatat ccgctaccac    120 

caatgcctaa tgcatttgga caagttgata ggatatactt tccaagatcg ttgtctgttg    180 

cagctggcca tgactcatcc aagtcatcat ttaaattttg gaatgaatcc tgatcatgcc    240 

aggaattcat tatctaactg tggaattcgg cagcccaaat acggagacag aaaagttcat    300 

cacatgcaca tgcggaagaa agggattaac accttgataa atatcatgtc acgccttggc    360 

caagatgacc caactccctc gaggattaac cacaatgaac ggttggaatt cctgggtgat    420 

gctgttgttg aatttctgac cagcgtccat ttgtactatt tgtttcctag tctggaagaa    480 

ggaggattag caacctatcg gactgccatt gttcagaatc agcaccttgc catgctagca    540 

aagaaacttg aactggatcg atttatgctg tatgctcacg ggcctgacct ttgtagagaa    600 

tcggaccttc gacatgcaat ggccaattgt tttgaagcgt taataggagc tgtttacttg    660 

gagggaagcc tggaggaagc caagcagtta tttggacgct tgctctttaa tgatccggac    720 

ctgcgcgaag tctggctcaa ttatcctctc cacccactcc aactacaaga gccaaatact    780 

gatcgacaac ttattgaaac ttctccggtt ctacaaaaac ttactgagtt tgaagaagca    840 

attggagtaa tttttactca tgttcgactt ctggcaaggg cattcacatt gagaactgtg    900 

ggatttaacc atctgaccct aggccacaat cagagaatgg aattcctagg tgactccata    960 

atgcaactgg tagccacaga gtacttattc attcatttcc cagatcatca tgaaggacac   1020 

ttaactttgt tgcgaagctc tttggtgaat aatagaactc aggccaaggt agcggaggag   1080 

ctgggcatgc aggagtacgc cataaccaac gacaagacca agaggcctgt ggcgcttcgc   1140 

accaagacct tggcggacct tttggaatca tttattgcag cgctgtacat tgataaggat   1200 

ttggaatatg ttcatacttt catgaatgtc tgcttctttc cacgattgaa agagttcatt   1260 

ttgaatcagg attggaatga ccccaaatcc cagcttcagc agtgttgctt gacacttagg   1320 

acagaaggaa aagagccaga cattcctctg tacaagactc tgcagacagt gggcccatcc   1380 

catgcccgaa cctacactgt ggctgtttat ttcaagggag aaagaatagg ctgtgggaaa   1440 

ggaccaagta ttcagcaagc ggaaatggga gcagcaatgg atgcgcttga aaaatataat   1500 

tttccccaga tggcccatca gaagcggttc atcgaacgga agtacagaca agagttaaaa   1560 

gaaatgaggt gggaaagaga gcatcaagag agagagccag atgagactga agacatcaag   1620 

aaataa                                                              1626