Patent Publication Number: US-9844778-B2

Title: Testing module and method for testing test sample

Description:
CROSS REFERENCE TO RELATED APPLICATIONS 
     This application claims priority of Taiwan Patent Application No. 103126547, filed on Aug. 4, 2014, the entirety of which is incorporated by reference herein. 
     BACKGROUND 
     Field of the Invention 
     The present invention relates to a testing module and a method of using the testing module, and more particularly to a testing module with a designed flow path for changing the process to mix a test sample and a fluid and a method for using the testing module. 
     Description of the Related Art 
     The process for testing a test sample typically includes the following steps (1) providing a test sample; (2) providing a fluid to dilute the test sample; (3) fully mixing the test sample and a reactive reagent; and (4) performing a measurement. A conventional testing module for testing the test sample for example in2it, a product of Bio-rad, includes a mixing chamber. To carry out the above-mentioned steps, the fluid and the test sample are respectively introduced into the mixing chamber and are mixed in the mixing chamber. However, the process is quite time-consuming and not easy to operate. 
     In addition, in the process of collecting the test sample by a conventional sampling member, it is inevitable that excess test sample adheres the outer surface of the sampling member. When carrying out the measurement, the above excess test sample causes changes in the amount of the specimen, and a measurement error may occur. 
     Consequently, it would be desirable to provide a solution for the testing module to test the test sample. 
     SUMMARY 
     Accordingly, one objective of the present invention is to provide a testing module which is adapted to test a test sample. One advantage of the test module is that it can be quickly operated. A further advantage of the test module is that the amount of the test sample can be controlled to improve the measurement accuracy. 
     According to some embodiments of the disclosure, the testing module includes a flow path, a storage chamber, a carrier, a block member, and a sampling assembly. The flow path is used to guide the flow of a fluid. The storage chamber is fluidly connected to an upstream of the flow path and configured to provide the fluid. The carrier has a mixing chamber. The mixing chamber is fluidly connected to a downstream of the flow path and used to receive the fluid and the test sample. The block member is disposed in the flow path and selectively transformed from a first state to a second state. The sampling assembly is detachably connected to the carrier and includes a sampling member used to collect the test sample. Before the sampling assembly is connected to the carrier, the block member is in the first state to block the fluid in the storage chamber flowing from the upstream of the flow path to the downstream of the flow path. After the sampling assembly is connected to the carrier, the block member is in the second state to enable the fluid in the storage chamber to flow from the upstream of the flow path to the downstream of the flow path, wherein at least a portion of the fluid flows into the downstream of the flow path via the sampling member and mixes with the test sample in the sampling member. 
     In some embodiments, a passage is formed in the sampling member, and the test sample is disposed in the passage. The passage includes a fluid inlet, configured to receive the fluid in the storage chamber; and a fluid outlet, configured to exhaust the fluid and the test sample to the downstream of the flow path. 
     In some embodiments, the testing module further includes a puncturing structure arranged relative to the block structure. The block structure includes a membrane. A bottom opening is formed on a lower surface of the storage chamber, and the membrane is connected to the storage chamber relative to the bottom opening. The puncturing structure is configured to penetrate the membrane. The first state refers to the membrane being intact without breakage, and the second state refers to an opening being formed on the membrane after the sampling assembly is connected to the carrier. 
     In some embodiments, a top opening is formed on an upper surface of the storage chamber, and another membrane is formed on the upper surface of the storage chamber relative to the top opening, the puncturing structure penetrates both of the membranes after the sampling assembly is connected to the carrier. 
     In some embodiments, the puncturing structure includes a piercing part and a depressed portion depressed from a lateral surface of the puncturing structure for allowing the fluid from the storage chamber passing therethrough. In some embodiments, the puncturing structure includes a bottom portion and a top portion disposed on the bottom portion and having the piercing part. The lateral surface relative to the top portion has an inclined surface, and the width of the top portion is varied. In some embodiments, the testing module further includes a supporting member disposed adjacent to the puncturing structure, and after the sampling assembly is connected to the carrier, the storage chamber abuts against the supporting member. 
     In some embodiments, the storage chamber includes a number of storage spaces secluded from each other. The number of the storage spaces corresponds to that of the puncturing structures, and each puncturing structure faces one of the storage spaces. In some embodiments, the puncturing structure and the sampling assembly are formed integrally and connected to the carrier in a detachable manner. 
     In some embodiments, at least one dent is formed on a circumferential surface of the sampling member and communicates with the passage, and the fluid inlet is formed relative to the at least one dent, and the fluid outlet is formed on a bottom surface of the sampling member. In some embodiments, the passage comprises another fluid inlet configured to receive the fluid in the storage chamber, and the number of the at least one dent is two, wherein the two dents are formed on two opposite sides of the circumferential surface of the sampling member, the two fluid inlets are respectively formed relative to the two dents. 
     In some embodiments, the carrier further comprises an accommodating space and a through hole fluidly connecting the mixing chamber and the accommodating space, wherein the storage chamber is placed in the accommodating space and the sampling assembly is disposed in the through hole when the sampling assembly is connected to the carrier. 
     In some embodiments, the block structure comprises a recess formed on an upper surface of the carrier, and when the sampling assembly is connected to the carrier, the sampling member is disposed in the recess, wherein a width of the sampling member is smaller than that of the block structure. 
     In some embodiments, the block structure comprises an opening penetrating the carrier, and a notch is formed in the vicinity of the block structure, wherein the sampling assembly further comprises a clamping structure, after the sampling assembly is connected to the carrier, the clamping structure engages with the notch, and the sampling assembly is disposed in the opening. In some embodiments, the testing module further includes a liquid-absorbing material disposed on a lower surface of the carrier relative to the opening. 
     In some embodiments, the sampling assembly comprises a supporting structure, wherein the sampling member is disposed on the supporting structure. The block structure includes a recess, formed on an upper surface of the carrier and including a bottom surface; and an opening, formed on a lower surface of the carrier and communicating with the recess. The sampling assembly is connected to the carrier through the opening, and the supporting structure abuts the bottom surface of the recess when the sampling member is placed in the flow path. In some embodiments, the bottom surface of the recess is an inclined surface. A region of the bottom surface of the recess which is adjacent to the upstream of the flow path is higher than another region of the bottom surface of the recess which is adjacent to the downstream of the flow path. 
     Another objective of the disclosure is to provide a method for testing a test sample. According to some embodiments of the disclosure, the method includes blocking a fluid from a storage chamber flowing into a mixing chamber via a flow path; collecting the test sample by a sampling assembly; placing the sampling assembly in the flow path; enabling the fluid to flow out of the storage chamber and to pass through the sampling assembly to mix with the test sample collected by the sampling assembly; and enabling the fluid mixed with the test sample to flow into the mixing chamber. 
     In some embodiments, the operation of driving the fluid to flow out of the storage chamber includes providing a centrifugal force or a pump so as to actuate the flow of the fluid. 
     In some embodiments, the fluid comprises a diluent or a reactive reagent, and the test sample comprises blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell sample, or any other bodily fluid, and the mixing chamber is formed in a carrier. 
     In some embodiments, the operation of blocking the fluid from the storage chamber flowing into the mixing chamber via the flow path comprises providing a block structure to block the storage chamber, forming an opening at the flow path, or forming a recess on the flow path. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
       For a more complete understanding of the embodiments, and the advantages thereof, reference is now made to the following descriptions taken in conjunction with the accompanying drawings. 
         FIG. 1  shows a block diagram of a testing module of the disclosure. 
         FIG. 2  shows a top view of the testing module of a first embodiment of the disclosure. 
         FIG. 3A  shows a schematic cross-sectional view of the testing module of the first embodiment of the disclosure taken along line A-A′ of  FIG. 2  with a block structure in a first state. 
         FIG. 3B  shows a schematic cross-sectional view of the testing module of the first embodiment of the disclosure taken along line A-A′ of  FIG. 2  with the block structure in a second state. 
         FIG. 4A  shows an exploded view of the testing module of a second embodiment of the disclosure. 
         FIG. 4B  shows a schematic cross-sectional view of a sampling assembly of a second embodiment of the disclosure. 
         FIG. 4C  shows a schematic view of a sampling assembly of the other embodiment of the disclosure. 
         FIG. 5  shows a top view of a portion of the testing module of the second embodiment of the disclosure. 
         FIG. 6A  shows a schematic cross-sectional view of the testing module of the second embodiment of the disclosure with a block structure in a first state. 
         FIG. 6B  shows a schematic cross-sectional view of the testing module of the second embodiment of the disclosure with the block structure in a first state. 
         FIG. 7  shows an exploded view of the testing module of a third embodiment of the disclosure. 
         FIG. 8  shows a top view of a portion of the testing module of the third embodiment of the disclosure. 
         FIG. 9  shows a schematic view of the sampling assembly of the third embodiment of the disclosure. 
         FIG. 10  shows a schematic cross-sectional view taken along line E-E′ of  FIG. 8 . 
         FIG. 11  shows an exploded view of a testing module of a fourth embodiment of the disclosure. 
         FIG. 12  shows a top view of a carrier of the fourth embodiment of the disclosure. 
         FIG. 13  shows a schematic view of a sampling assembly of the fourth embodiment of the disclosure. 
         FIGS. 14A-14C  show top views of operations of connecting the sampling assembly to the carrier of the fourth embodiment of the disclosure. 
         FIG. 15  shows a schematic cross-sectional view of a portion of the testing assembly of the fourth embodiment of the disclosure taken along line C-C′ of  FIG. 14C . 
         FIG. 16A  shows an exploded view of a testing module of a fifth embodiment of the disclosure. 
         FIG. 16B  shows a schematic view of partial of a carrier of a fifth embodiment of the disclosure. 
         FIG. 16C  shows a side view of partial of a carrier of a fifth embodiment of the disclosure observed from line D-D′ of  FIG. 16A . 
         FIG. 17  shows a schematic view of a portion of the testing assembly of the fifth embodiment of the disclosure. 
         FIG. 18  shows a schematic view after the testing assembly connecting with the carrier of the fifth embodiment of the disclosure. 
         FIG. 19  shows a schematic view of the testing module disposed on a rotation plate in accordance with the fifth embodiment of the disclosure. 
         FIG. 20  shows a schematic view of a portion of a testing module of a sixth embodiment of the disclosure. 
         FIG. 21  shows a schematic view of a portion of the testing module of the sixth embodiment of the disclosure. 
     
    
    
     DESCRIPTION OF THE ILLUSTRATIVE EMBODIMENTS 
     The following description is of the best-contemplated mode of carrying out the invention. This description is made for the purpose of illustrating the general principles of the invention and should not be taken in a limiting sense. The scope of the invention is best determined by reference to the appended claims. 
       FIG. 1  shows a block diagram of a testing module  1  of the disclosure. According to the disclosure, the testing module  1  which is adapted to test a test sample F 2  includes a storage chamber  110 , a mixing chamber  150 , a flow path  130 , a block structure  200 , and a sampling assembly  300 . The storage chamber  110  is fluidly connected to the mixing chamber  150  via the flow path  130 . In one embodiment, the storage chamber  110  holds a fluid F 1 , and the mixing chamber  150  holds a reactive reagent F 3 . The block structure  200  is disposed in the flow path  130  and configured to block the fluid F 1  of the storage chamber  110  from flowing into the mixing chamber  150  before the placing of the sampling assembly  300  into the flow path  130 . The sampling assembly  300  is configured to collect the test sample F 2  for test. After the placing of the sampling assembly  300  in the flow path  130  corresponding to the block structure  200 , the fluid F 1  in an upstream  131  of the flow path  130  flows to a downstream  133  of the flow path  130  via the sampling assembly  300 . In addition, due to the earlier mixing of the fluid F 1  and the test sample F 2  before flowing into the mixing chamber  150 , the process for testing the test sample F 2  is simplified. 
     First Embodiment 
       FIG. 2  shows a top view of the testing module  1   a  of the first embodiment of the disclosure. According to the first embodiment of the disclosure, the testing assembly  1   a  includes a carrier  100   a  and a block structure  200   a . In the first embodiment, a storage chamber  110   a , a flow path  130   a , and a mixing chamber  150   a  are respectively formed on an upper surface  101   a  of the carrier  100   a . The storage chamber  110   a  and the mixing chamber  150   a  are separated from each other and fluidly connected to each other via the flow path  130   a . In this embodiment, the position of the storage chamber  110   a  is closer to a substantial center C of the carrier  100   a  than that of the mixing chamber  150   a . The storage chamber  110   a  may be used to hold a fluid F 1 , such as salt water or another diluent. The mixing chamber  150   a  may be used to hold a reactive reagent F 3 , such as reactive material. The block structure  200   a  is a recess formed on the upper surface  101   a  of the carrier  100   a  and disposed between an upstream  131   a  and a downstream  133   a  of the flow path  130   a.    
       FIG. 3A  shows a schematic cross-sectional view of the testing module  1   a  of the first embodiment of the disclosure taken along line A-A′ of  FIG. 2 . According to the first embodiment of the disclosure, the testing module  1   a  further includes a sampling assembly  300   a . In this embodiment, the sampling assembly  300   a  includes a seat  310   a , a sampling member  330   a  and a handle  350   a . The sampling member  330   a  and the handle  350   a  are respectively disposed on two opposite sides of the seat  310   a . The handle  350   a  is configured to facilitate the holding of a manipulator or a robotic arm. A passage  370   a  is formed in the sampling member  330   a , wherein a fluid inlet  371   a  and a fluid outlet  373   a  located at two ends of the passage  370   a  are respectively formed on two opposite lateral surfaces  331   a  and  333   a  of the sampling member  330   a . The passage  370   a  is adapted to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell test sample, or any other bodily fluid. 
     The operation method of testing the test sample F 2  by the testing module  1   a  according to the first embodiment of the disclosure is described below. 
     In the beginning, as shown in  FIG. 3A , the fluid F 1  is provided in the storage chamber  110   a , and the reactive reagent F 3  is provided in the mixing chamber  150   a . Before the combination of the sampling assembly  300   a  and the carrier  100   a , the block structure  200   a  is in a first state, in which the block structure  200   a  is not closed. The fluid F 1  may flow out of the storage chamber  110   a  due to a swinging motion of the carrier  100   a . However, because the block structure  200   a  is in the first state, the fluid F 1  is held in the block structure  200   a  and is limited not to flow into the mixing chamber  150   a  via the flow path  130   a . Therefore, the reactive reagent F 3  is prevented from being contaminated by the fluid F 1 . 
     Afterwards, as shown in  FIG. 3A , the test sample F 2  is collected in the passage  370   a  by the sampling assembly  300   a  and kept in the passage  370   a  via capillary force. 
     Afterwards, the sampling assembly  300   a  is transported and combined to the carrier  100   a , wherein the sampling assembly  300   a  is placed in the flow path  130   a  corresponding to the block structure  200   a . At this moment, the block structure  200   a  is in a second state, in which the block structure  200   a  is closed by the seat  310   a . The sampling assembly  300   a  and the carrier  100   a  are combined through means including gluing and clamping. The sampling assembly  300   a  and the carrier  100   a , shown in  FIG. 3B , are connected by gluing. 
     Afterwards, as shown in  FIG. 3B , after the connection of the sampling assembly  300   a  and the carrier  100   a , the seat  310   a  of the sampling assembly  300   a  is supported by the upper surface  101   a  of the carrier  100   a , and the sampling member  330   a  of the sampling assembly  300   a  is placed in the block structure  200   a . It should be noted that along substantially an extension direction X of the flow path  130   a , the width W 1  of the sampling member  330   a  is smaller than the width W 2  of the block structure  200   a . In addition, a gap g is formed between a lower surface  335   a  of the sampling member  330   a  and a bottom surface  201   a  of the block structure  200   a  to allow the fluid F 1  to pass therethrough. 
     Afterwards, the fluid F 1  is driven to flow from the storage chamber  110   a  to the sampling assembly  300   a , and the fluid F 1  is mixed with the test sample F 2  collected by the sampling assembly  300   a . Specifically, the fluid F 1  is driven to flow out of the storage chamber  110   a  by applying an external force and to flow to the block structure  200   a  via the upstream  131   a . After the fluid F 1  flows into the block structure  200   a , a portion of the fluid F 1  flows to the downstream  133   a  via the gap g between the sampling member  330   a  and the block structure  200   a , and the other portion of the fluid F 1  flows to the downstream  133   a  via the passage  370   a  and mixes with the test sample F 2  in the passage  370   a . Generally, the viscosity of the fluid F 1  is lower than that of the test sample F 2  so as to facilitate the fluid F 1  flushing the test sample F 2  out of the passage  370   a ; however, the embodiment should not be limited thereto. The viscosity of the fluid F 1  may be higher than or equal to that of the test sample F 2  and the fluid F 1  will enter the passage  370   a  and bring the test sample F 2  to the mixing chamber  150   a.    
     Afterwards, the fluid F 1  is driven to flow into the mixing chamber  150   a  via the downstream  133   a . At this moment, since the fluid F 1  has been already mixed with the test sample F 2  before flowing into the mixing chamber  150   a , the test sample F 2  immediately reacts with the reactive reagent F 3  once that the fluid F 1  flows into the mixing chamber  150   a . Last, after the reaction of the test sample F 2  and the reactive reagent F 3  is finished, a measurement of the reaction result is performed. The process of testing the test sample F 2  is completed. 
     In the first embodiment, the operation of driving the fluid F 1  to flow out of the storage chamber  110   a  includes rotating the carrier  100   a  about the substantial center C of the carrier  100   a  to generate a centrifugal force to drive the fluid F 1  to flow. In another embodiment, the operation of driving the fluid F 1  to flow out of the storage chamber  110   a  includes providing a pump to drive the fluid F 1  to flow. 
     Second Embodiment 
       FIG. 4A  shows an exploded structural view of the testing module  1   b  of a second embodiment of the disclosure  FIG. 4B  shows a schematic cross-sectional view of a sampling assembly  300   b  of a second embodiment of the disclosure.  FIG. 4C  shows a schematic view of a sampling assembly  300   b ′ of the other embodiment of the disclosure. In the second embodiment, the testing assembly  1   b  includes a carrier  100   b  and a block structure  200   b , and a sampling assembly  300   b.    
     The carrier  100   b  includes a base  120   b , an accommodating space  123   b , a storage chamber  110   b , a mixing chamber  150   b , and a cover  160   b . The accommodating space  123   b  is formed at an upper surface  121   b  of the base  120   b . The accommodating space  123   b  has a shape which conforms to the shape of the storage chamber  110   b  such that the storage chamber  110   b  can be placed in the accommodating space  123   b . The mixing chamber  150   b  is formed on the upper surface  121   b  of the base  120   b  and arranged adjacent to the accommodating space  123   b . The accommodating space  123   b  communicates with the mixing chamber  150   b  via a flow path  130   b.    
     The storage chamber  110   b  is a hollow case, a top opening  112   b  is formed on an upper surface  111   b  of the storage chamber  110   b . A membrane  180   b  is placed on the upper surface  111   b  relative to the top opening  112   b . The membrane  180   b  may be a metallic membrane (such as an aluminum membrane) or a plastic membrane and may be connected to the edge of the upper surface  111   b  of the storage chamber  110   b  by ultrasonic fusing, heat sealing, or laser radiation. A bottom opening  114   b  is formed on a lower surface  113   b  of the storage chamber  110   b . The block structure  200   b  is placed on the lower surface  113   b  of the storage chamber  110   b  relative to the bottom opening  114   b . In the second embodiment, the block structure  200   b  is a membrane, such as an aluminum membrane. The block structure  200   b  may be placed on the lower surface  113   b  of the storage chamber  110   b  by ultrasonic fusing, heat sealing, or laser radiation. 
     The cover  160   b  is disposed on the base  120   b , so as to fix the storage chamber  110   b  in the base  120   b . A guiding hole  161   b  is formed on the cover  160   b  relative to the top opening  112   b  to facilitate the passing of the sampling assembly  300   b.    
     As shown in  FIG. 4B , the sampling assembly  300   b  includes a seat  310   b  and a sampling member  330   b  connected to the seat  310   b . The sampling member  330   b  has a bottom surface  331   b  with a puncturing structure  335   b . A passage  370   b  is formed in the sampling member  330   b , wherein a fluid inlet  371   b  of the passage  370   b  is formed at the circumferential surface  337   b  of the sampling member  330   b , and a fluid outlet  373   b  of the passage  370   b  is formed at the bottom surface  331   b  of the sampling member  330   b . The passage  370   b  is used to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell sample, or any other bodily fluid through capillary force. However, the structural feature of the sampling assembly  300   b  should not be limited to the above embodiment. 
     As shown in  FIG. 4C , in the other embodiment, the sampling assembly  300   b ′ includes a seat  310   b , and a sampling member  330   b ′ connected to the seat  310   b . The sampling member  330   b ′ has a columnar structure with a bottom surface  331   b ′. Two dents  375   b ′ are formed on a circumferential surface  337   b ′ and located on two opposite sides of the sampling member  330   b ′. A passage  370   b ′ is connected between and communicates with the two dents  375   b ′. The passage  370   b ′ has two fluid inlets  371   b ′ formed relative to the dents  375   b ′, and the passage  370   b ′ has a fluid outlet  373   b ′ formed on the bottom surface  331   b ′ of the sampling member  330   b ′. The passage  370   b ′ is used to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell sample, or any other bodily fluid through capillary force. Since the two fluid inlets  371   b ′ are respectively formed in the dents  375   b ′, the test sample F 2  is kept within the passage  370   b ′ and kept from being in contact with other elements and from being released during an insertion process of the sampling assembly  300   b ′ into the storage chamber  110   b . In some other embodiments, the number of the dent  375   b ′ may be one. and the passage  370   b ′ has one fluid inlet  371   b ′ formed relative to the dents  375   b , and the passage  370   b ′ has a fluid outlet  373   b ′ formed on the bottom surface  331   b ′ of the sampling member  330   b′.    
       FIG. 5  shows a top view of a portion of the structure of the testing module  1   b  of the second embodiment of the disclosure. In the second embodiment, a flow path  130   b  is formed in the testing assembly  1   b . Specifically, an upstream  131   b  of the flow path  130   b  is formed in the storage chamber  110   b , and a downstream  133   b  of the flow path  130   b  is formed in the base  120   b . In addition, the storage chamber  110   b  is fluidly connected to the upstream  131   b , and the mixing chamber  150   b  is fluidly connected to the downstream  133   b . The storage chamber  110   b  may be used to hold a fluid F 1 , such as salt water or another diluent. The mixing chamber  150   b  may be used to hold a reactive reagent F 3 , such as reactive material. Referring to  FIGS. 5 and 6A ,  FIG. 6A  shows a schematic cross-sectional view of the testing module  1   b  of the second embodiment of the disclosure taken along line B-B′ of  FIG. 5 . The operation method of testing the test sample F 2  by the testing module  1   b  according to the second embodiment of the disclosure is described below. 
     In the beginning, as shown in  FIG. 5 , the fluid F 1  is provided in the storage chamber  110   b , and the reactive reagent F 3  is provided in the mixing chamber  150   b . As shown in  FIG. 6A , before the connection of the sampling assembly  300   b  and the carrier  100   b , the block structure  200   b  is in a first state, in which the membrane (the block structure  200   b ) is intact without breakage. Therefore, the storage chamber  110   b  is sealed by the membrane  180   b  and the block structure  200   b , and the fluid F 1  is safely held in the storage chamber  110   b.    
     Afterwards, as shown in  FIG. 6A , the test sample F 2  is collected in the passage  370   b  by the sampling assembly  300   b  and kept in the passage  370   b  through capillary force. 
     Afterwards, the sampling assembly  300   b  is transported and connected to the carrier  100   b , wherein the sampling assembly  300   b  is inserted into the sampling assembly  100   b  and guided by the guiding hole  161   b  of the cover  160   b , and therefore the sampling assembly  300   b  is engaged on the cover  160   b.    
     Afterwards, as shown in  FIG. 6B , after the connection of the sampling assembly  300   b  and the carrier  100   b , the sampling member  330   b  is disposed in the flow path  130   b , and the membrane  180   b  and the block structure  200   b  relative to the guiding hole  161   b  are piercingly penetrated by the puncturing structure  335   b  of the sampling member  330   b . At this moment, the block structure  200   b  is in a second state, in which the membrane (the block structure  200   b ) is not intact and has a through hole due to being pierced. The fluid F 1  flows out of the storage  110   b  via the bottom opening  114   b , wherein the fluid F 1  can naturally flow out of the storage chamber  110   b  through the force of gravity. 
     It should be noted that when the fluid F 1  flows out of the storage  110   b , a portion of the fluid F 1  flows out of the storage chamber  110   b  via a slit between the sampling member  330   b  and the bottom opening  114   b , and the other portion of the fluid F 1  flows out of the storage chamber  110   b  via the passage  370   b  and mixes with the test sample F 2  in the passage  370   b . Specifically, the fluid F 1  flowing through the passage  370   b  enters the passage  370   b  via the fluid inlet  371   b  and leaves the passage  370   b  via the fluid outlet  373   b  together with the test sample F 2 . In the embodiment, the portion of the flow path  130   b  of the fluid F 1  flowing from the storage chamber  110  to the fluid outlet  373   b  via the fluid inlet  371   b  is referred to as the upstream  131   b , and the other portion of the flow path  130  of the fluid F 1  and the test sample F 2  flowing from the fluid outlet  373   b  to the mixing chamber  150   b  is referred to as the downstream  133   b . The viscosity of the fluid F 1  is lower than that of the test sample F 2  so as to facilitate the fluid F 1  flushing the test sample F 2  out of the passage  370   b ; however, the embodiment should not be limited thereto. The viscosity of the fluid F 1  may be higher than or equal to that of the test sample F 2 , and the fluid F 1  will also enter the passage  370   b  and bring the test sample F 2  to the mixing chamber  150   b.    
     Referring again to  FIG. 5 , after the fluid F 1  flows out of the storage chamber  110   b , the fluid F 1  is driven to flow into the mixing chamber  150   b  via the downstream  133   b . At this moment, since the fluid F 1  has been already mixed with the test sample F 2  before flowing into the mixing chamber  150   b , the test sample F 2  immediately reacts with the reactive reagent F 3  once that the fluid F 1  flows into the mixing chamber  150   b . Last, after the reaction of the test sample F 2  and the reactive reagent F 3  is finished a measurement of the reaction result is performed. Therefore, the process of testing the test sample F 2  is completed. 
     In the second embodiment, the operation of driving the fluid F 1  to flow into the mixing chamber  150   b  includes placing the carrier  100   b  as a whole on a rotation plate (not shown), wherein the storage chamber  110   b  is closer to a rotation center of the rotation plate than the mixing chamber  150   b . Afterwards, the rotation plate is rotated to generate a centrifugal force to drive the fluid F 1  to flow. In another embodiment, the operation of driving the fluid F 1  to flow out of the storage chamber  110   b  includes providing a pump to drive the fluid F 1  to flow. 
     Third Embodiment 
       FIG. 7  shows an exploded structural view of the testing module  1   c  of a third embodiment of the disclosure, and  FIG. 8  shows a top view of a portion of the structure of the testing module  1   c  of the third embodiment of the disclosure. In the third embodiment, the testing module  1   c  includes a carrier  100   c , a block structure  200   c , and one or more sampling assemblies  300   c.    
     As shown in  FIG. 8 , a storage chamber  110   c , a flow path  130   c , and a mixing chamber  150   c  are respectively formed on an upper surface  101   c  of the carrier  100   c . The storage chamber  110   c  and the mixing chamber  150   c  are separated from each other and fluidly connected to each other via the flow path  130   c . In the embodiment, the position of the storage chamber  110   c  is closer to a substantial center C of the carrier  100   c  than that of the mixing chamber  150   c . The storage chamber  110   c  may be used to hold a fluid F 1 , such as salt water or another diluent. The mixing chamber  150   c  may be used to hold a reactive reagent F 3 , such as reactive material. In some embodiments, the testing module  1   c  further includes a cover or a membrane (not shown in the Figures) to seal the upper surface  101   c  of the carrier  100   c.    
     The block structure  200   c  is an opening penetrating the upper and lower surfaces of the carrier  100   c  and disposed between an upstream  131   c  and a downstream  133   c  of the flow path  130   c . The opening  200   c  has a shape compatible with the shape of the sampling assemblies  300   c . In addition, as shown in  FIG. 7 , in the vicinity of the block structure  200   c , a pair of notches  170   c  is arranged, and a liquid-absorbing material  400   c  is placed on the lower surface  102   c  of the carrier  100   c  relative to the block structure  200   c . The liquid-absorbing material  400   c  (such as sponge, velvet, non-woven fabric, cotton paper) includes a plurality of central slits  410   c  formed thereon to allow the sampling assembly  300   c  to pass therethrough. The functions of the notches  170   c  and the liquid-absorbing material  400   c  will be described later. 
       FIG. 9  shows a schematic view of the sampling assembly  300   c  of the third embodiment of the disclosure. According to the third embodiment, the sampling assembly  300   c  includes a seat  310   c , a supporting structure  320   c , a sampling member  330   c , two clamping structures  340   c  and a sealing member  360   c . The supporting structure  320   c  and the two clamping structures  340   c  are disposed on the seat  310   c  and protrude from the seat  310   c  along the same direction. Specifically, the supporting structure  320   c  is disposed on a substantial center of the seat  310   c , and the two clamping structures  340   c  are respectively disposed on two opposite sides of the supporting structure  320   c  and adjacent to the lateral edges  311   c  and  312   c  of the seat  310   c.    
     The supporting structure  320   c  includes a first portion  321   c  and a second portion  323   c . The first portion  321   c  is disposed on the seat  310   c , and the second portion  323   c  is disposed on the first portion  321   c . The cross-sectional area of the second portion  323   c  is larger than that of the first portion  321   c . The sealing member  360   c  is disposed on the first portion  321   c  and completely surrounds the peripheral of the second portion  323   c . The sampling member  330   c  is disposed on the second portion  323   c . A passage  370   c  is formed in the center of the sampling member  330   c . The passage  370   c  is used to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell test sample, or any other bodily fluid. A fluid inlet  371   c  and a fluid outlet  373   c  are formed at two end of the passage  370   c , and fluid can flow through the passage  370   c  via the fluid inlet  371   c  and the fluid outlet  373   c . In some embodiments, the positions of the fluid outlet  373   c  and the fluid inlet  371   c  may be inter changed. 
     The operation method of testing the test sample F 2  by the testing module  1   c  according to the third embodiment of the disclosure is described below. 
     Referring again to  FIG. 8 , in the beginning, the fluid F 1  is provided in the storage chamber  110   c , and the reactive reagent F 3  is provided in the mixing chamber  150   c . In the third embodiment, before the connection of the sampling assembly  300   c  and the carrier  100   c , the block structure  200   c  is in a first state, in which the block structure  200   c  is not closed. In some embodiments, the storage chamber  110   c  is lower than the flow path  130   c  (such as the structural features of the storage chamber  110   a  and the flow path  130   a  shown in  FIG. 3A ), so that the fluid F 1  is prevented from flowing out of the storage chamber  110   c . The fluid F 1  may flow out of the storage chamber  110   c  due to a swinging motion of the carrier  100   c . However, due to the arrangement of the block structure  200   c , the fluid F 1  is released via the block structure  200   c  and is absorbed by the liquid-absorbing material  400   c  and thus is limited not to flow into the mixing chamber  150   c  via the flow path  130   c . Therefore, the reactive reagent F 3  can be prevented from being contaminated by the fluid F 1 . 
     Afterwards, the test sample F 2  is collected in the passage  370   c  by the sampling assembly  300   c  and kept in the passage  370   c  through capillary force. Afterwards, the sampling assembly  300   c  is transported to connect to the carrier  100   c.    
     Specifically, as shown in  FIG. 10 , during the connection of the sampling assembly  300   c  to the carrier  100   c , the supporting structure  320   c  and the sampling member  330   c  are inserted into the block structure  200   c , and the two clamping structure  340   c  are respectively inserted in to the two notches  170   c . Since the supporting structure  320   c  and the sampling member  330   c  first pass through the central slits  410   c  of the liquid-absorbing material  400   c  before reaching into the block structure  200   c , the excess test sample F 2  on the sampling member  330   c  is absorbed by the liquid-absorbing material  400   c . This arrangement is such that the precision of the test result can be improved. 
     After the sampling assembly  300   c  is completely connected to the carrier  100   c , the two clamping structures  340   c  are respectively engaged with the two notches  170   c , and the sampling member  330   c  is disposed in the flow path  130   c . In addition, the sealing member  360   c  is deformed due to compression of an inner wall of the block structure  200   c . At this moment, the block structure  200   c  is in a second state, in which the block structure  200   c  is sealed by the sampling assembly  300   c.    
     Afterwards, as shown in  FIG. 8 , when the block structure  200   c  is in the second state, the fluid F 1  is driven to flow from the storage chamber  110   c  to the sampling assembly  300   c  and mixed with test sample F 2  collected by the sampling assembly  300   c . Specifically, the fluid F 1  is driven to flow out of the storage chamber  110   c  and pass through the upstream  131   c , the sampling assembly  300   c , and the downstream  133   c  before flowing into the mixing chamber  150   c.    
     It should be noted that when the fluid F 1  passes through the sampling assembly  300   c , a portion of the fluid F 1  flows to the downstream  133   c  via an slit between the sampling member  330   c  and an inner wall of the flow path  130   c , and the other portion of the fluid F 1  flows to the downstream  133   c  via the passage  370   c  ( FIG. 9 ) and mixes with the test sample F 2  in the passage  370   c . Specifically, the fluid F 1  enters the passage  370   c  via the fluid inlet  371   c  ( FIG. 9 ) of the passage  370   c  and leaves the passage  370   c  via the fluid outlet  373   c  ( FIG. 9 ) of the passage  370   c  together with the test sample F 2 . Since the fluid F 1  has been already mixed with the test sample F 2  before flowing into the mixing chamber  150   c , the test sample F 2  immediately reacts with the reactive reagent F 3  once that the fluid F 1  flows into the mixing chamber  150   c . Last, after the reaction of the test sample F 2  and the reactive reagent F 3  is finished a measurement of the reaction result is performed. The process of testing the test sample F 2  is completed. 
     In the third embodiment, the operation of driving the fluid F 1  to flow out of the storage chamber  110   c  includes rotating the carrier  100   c  about the substantial center C of the carrier  100   c  to generate a centrifugal force to drive the fluid F 1  to flow. In another embodiment, the operation of driving the fluid F 1  to flow out of the storage chamber  110   c  includes providing a pump to drive the fluid F 1  to flow. 
     Fourth Embodiment 
       FIG. 11  shows an exploded structural view of the testing module  1   d  of a fourth embodiment of the disclosure, and  FIG. 12  shows a top view of a portion of the structure of the testing module  1   d  of the fourth embodiment of the disclosure. In the fourth embodiment, the testing assembly  1   d  includes a carrier  100   d , a block structure  200   d , and a sampling assembly  300   d.    
     As shown in  FIG. 12 , a storage chamber  110   d , a flow path  130   d , and a mixing chamber  150   d  are respectively formed on an upper surface  101   d  of the carrier  100   d . The storage chamber  110   d  and the mixing chamber  150   d  are separated from each other and fluidly connected to each other via the flow path  130   d . In the embodiment, the position of the storage chamber  110   d  is closer to a substantial center C of the carrier  100   d  than that of the mixing chamber  150   d . The storage chamber  110   d  may be used to hold a fluid F 1 , such as salt water or another diluent. The mixing chamber  150   d  may be used to hold a reactive reagent F 3 , such as reactive material. In some embodiments, the testing module  1   d  further includes a cover or a membrane (not shown in the Figures) to seal the upper surface  101   d  of the carrier  100   d.    
     The block structure  200   d  includes a recess  210   d  and an opening  230   d . The recess  210   d  is formed on the upper surface  101   d  of the carrier  100   d  and positioned between an upstream  131   d  and a downstream  133   d  of the flow path  130   d  and has a bottom surface  215 . The opening  230   d  is formed at the lower surface  102   d  of the carrier  100   d  and penetrates the lower surface  102   d  of the carrier  100   d  and the bottom surface  215  of the recess  210   d  and has a substantially L-shape and communicates with the recess  210   d.    
       FIG. 13  shows a schematic view of the sampling assembly  300   d  of the fourth embodiment of the disclosure. According to the fourth embodiment, the sampling assembly  300   d  includes a seat  310   d , a supporting structure  320   d , a sampling member  330   d , and a handle  350   d  ( FIG. 11 ). The supporting structure  320   d  is disposed on the seat  310   d  and protrudes from the seat  310   d  along a predetermined direction. In the fourth embodiment, the supporting structure  320   d  further includes a cylinder  321   d  and a protrusion  324   d  radially protruding from the vicinity of a distal end of the cylinder  321   d , wherein the sampling member  330   d  is disposed on the protrusion  324   d . A passage  370   d  is formed in the center of the sampling member  330   d . The passage  370   d  is used to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell sample, or any other bodily fluid. A fluid inlet  371   d  and a fluid outlet  373   d  are formed at two end of the passage  370   d , and fluid can flow through the passage  370   d  via the fluid inlet  371   d  and the fluid outlet  373   d . In some embodiments, the testing module  1   d  further includes a liquid-absorbing material (as the liquid-absorbing material  400   c  shown in  FIG. 7 ) disposed on the lower surface  102   d  of the carrier  100   d  relative to the opening  230   d  of the block structure  200   d  to absorb excess test sample on the sampling assembly  300   d.    
     The operation method of testing the test sample F 2  by the testing module  1   d  according to the fourth embodiment of the disclosure is described below. 
     Referring again to  FIG. 12 , in the beginning, the fluid F 1  is provided in the storage chamber  110   d , and the reactive reagent F 3  is provided in the mixing chamber  150   d . In the fourth embodiment, before connecting the sampling assembly  300   d  to the carrier  100   d  through the opening  230   d  at the lower surface  102   d  of the carrier  100   d , the block structure  200   d  is in a first state, in which the block structure  200   d  is not closed. In the embodiment, the storage chamber  110   d  is lower than the flow path  130   d  (such as the structural features of the storage chamber  110   a  and the flow path  130   a  shown in  FIG. 3A ), so that the fluid F 1  is prevented from flowing out of the storage chamber  110   d . The fluid F 1  may flow out of the storage chamber  110   d  due to a swinging motion of the carrier  100   d . However, due to the arrangement of the block structure  200   d  in which the recess  210   d  is lower than the flow path  130   d , the fluid F 1  may be released via the opening  230   d  of the block structure  200   d  and may be absorbed by the liquid-absorbing material and is limited not to flow into the mixing chamber  150   d  via the flow path  130   d . Therefore, the reactive reagent F 3  can be prevented from being contaminated by the fluid F 1 . 
     Referring to  FIGS. 14A-14C , afterwards, the test sample F 2  is collected in the passage  370   d  by the sampling assembly  300   d  and kept in the passage  370   d  through capillary force. Afterwards, the sampling assembly  300   d  is transported and connected to the carrier  100   d . The method for connecting the sampling assembly  300   d  and the carrier  100   d  is described below. First, as shown in  FIG. 14A , insert the supporting structure  320   d  and the sampling member  330   d  into the through hole  230   d  of the block structure  200   d . Afterwards, as shown in  FIG. 14B , the sampling assembly  300   d  is rotated until the sampling member  330   d  abuts the inner wall  211   d  of the c and the sampling member  330   d  is placed in the flow path  130   d . At this moment, the block structure  200   d  is in a second state, in which the sampling member  330   d  is positioned between the upstream  131   d  and the downstream  133   d  of the flow path  130   d . Afterwards, as shown in  FIG. 14C , the fluid F 1  is driven to flow from the storage chamber  110   d  to the sampling assembly  300   d  and mixed with the test sample F 2  collected by the sampling assembly  300   d . Specifically, the fluid F 1  is driven to flow out of the storage chamber  110   d  and pass through the upstream  131   d , the sampling assembly  300   d , and the downstream  133   d  before flowing into the mixing chamber  150   d.    
     It should be noted that when the fluid F 1  passes through the sampling assembly  300   d , a portion of the fluid F 1  flows to the downstream  133   d  via an slit  213   d  between the sampling member  330   d  and the inner wall  211   d  of the flow path  130   d , and the other portion of the fluid F 1  flows to the downstream  133   d  via the passage  370   d  ( FIG. 13 ) and mixes with the test sample F 2  in the passage  370   d . Specifically, the fluid F 1  enters the passage  370   d  via the fluid inlet  371   d  ( FIG. 13 ) of the passage  370   d  and leaves the passage  370   d  via the fluid outlet  373   d  ( FIG. 13 ) of the passage  370   d  together with the test sample F 2 . Since the fluid F 1  has been already mixed with the test sample F 2  before flowing into the mixing chamber  150   d , the test sample F 2  immediately reacts with the reactive reagent F 3  once that the fluid F 1  flows into the mixing chamber  150   d . Last, after the reaction of the test sample F 2  and the reactive reagent F 3  is finished a measurement of the reaction result is performed. The process of testing the test sample F 2  is completed. 
       FIG. 15  shows a schematic cross-sectional view of a portion of the structure of the testing assembly  1   d  of the fourth embodiment of the disclosure taken along line C-C′ of  FIG. 14C . In some embodiments, the protrusion  324   d  and the seat  310   d  is spaced by a distance H 1 , and the bottom surface  215  of the recess  210   d  and the lower surface  102   d  of the carrier  100   d  is spaced by a distance H 2 . The distance H 1  may be greater than or equal to the distance H 2 . The bottom surface  215   d  of the recess  210   d  includes an inclined surface. The distance H 2  between the bottom surface  215   d  of the recess  210   d  and the lower surface  102   d  of the carrier  100   d  is varied. For example, a region of the bottom surface  215   d  adjacent to the upstream  131   d  is higher than another region of the bottom surface  215   d  adjacent to the downstream  133   d , and a height difference H 3  is defined between the two regions. With the height difference H 3 , the sampling assembly  300   d  may smoothly rotate within the recess  210   d  of the carrier  100   d , and after the rotation of the sampling assembly  300   d  on the carrier  100   d , the protrusion  324   d  abuts the bottom surface  215   d  of the recess  210   d  tightly, and the sampling assembly  300   d  is prevented from being dropped. The sampling assembly  300   d  is firmly engaged with the carrier  100   d.    
     Fifth Embodiment 
       FIG. 16A  shows an exploded structural view of a testing module  1   e  of the fifth embodiment of the disclosure. In the fifth embodiment, the testing module  1   e  includes a carrier  100   e , a storage chamber  110   e , a cover  160   e , a block structure  200   e , and a sampling assembly  300   e.    
     The carrier  100   e  includes a base  120   e , an accommodating space  123   e , a mixing chamber  150   e , and one or more pyramid shaped puncturing structures  105   e . The accommodating space  123   e  is formed on an upper surface of the base  120   e  and arranged adjacent to a top lateral edge  1231   e  of the base  120   e . The mixing chamber  150   e  is formed on the upper surface of the base  120   e  and arranged adjacent to the accommodating space  123   e . The accommodating space  123   e  communicates with the mixing chamber  150   e  via a through hole  107   e . The cover  160   e  covers the upper surface of the base  120   e , so as to seal the accommodating space  123   e  and the mixing chamber  150   e.    
     The puncturing structures  105   e  are positioned in the accommodating space  123   e  and extend toward the top lateral edge  1231   e  and terminate at its end portion. As shown in  FIG. 16B , each of the puncturing structures  105   e  includes a bottom portion  1054   e  and a top portion  1052   e  positioned on the bottom portion  1054   e . The top portion  1052   e  has a triangular cross section shape and has a piercing part. However, the shape of the top portion  1052   e  can be made in any shape as long as there is a piercing part formed thereon. In addition, as shown in  FIG. 16C , a lateral surface  1053   e  relative to the top portion  1052   e  is an inclined surface. Therefore, the width of the top portion  1052   e  is varied. For example, the width of the top portion  1052   e  is increased from a width W 1  to a width W 2  along a direction toward the bottom portion  1054   e . In other embodiments, the width W 1  may be equal to or greater than the width W 2 . In some embodiments, each of the puncturing structures  105   e  has a depressed portion  1051   e  depressed from the lateral surface  1053   e  of the puncturing structures  105   e  for allowing fluid passing therethrough and for facilitating the flowing of the fluid out of the storage chamber. The depressed portion  1051   e  has a depth of W 3  which is smaller than or equal to the width W 2 . In addition, a supporting member  108   e  ( FIG. 16B ) is formed between the puncturing structures  105   e  to support the storage chamber  110   e  after the storage chamber  110   e  enters the accommodating space  123   e.    
     Referring to  FIG. 17 , in some embodiments, the storage chamber  110   e  includes a number of storage spaces, such as the storage spaces  110   e   1  and  110   e   2 . The storage spaces  110   e   1  and  110   e   2  are secluded by each other. The storage spaces  110   e   1  and  110   e   2  may be used to hold the same or different fluid. For example, in the embodiment shown in  FIG. 17 , the storage space  110   e   1  holds the fluid F 1 , such as a reactive reagent, and the storage space  110   e   2  holds the fluid F 1 ′, such as a diluent. In some embodiments, the storage chamber  110   e  includes only one storage space with one fluid, and the selection of liquid in the mixing chamber  150   e  is determined according to the liquid held by the storage chamber  110   e . For example, the mixing chamber  150   e  may hold reactive reagents. Alternatively, there is no liquid in the mixing chamber  150   e . A bottom opening  112   e  is formed on a lower surface  111   e  of the storage chamber  110   e . The block structure  200   e  is formed on the lower surface  111   e  of the storage chamber  110   e  relative to the bottom opening  112   e . In the fifth embodiment, the block structure  200   e  is a membrane, such as an aluminum membrane. The block structure  200   e  may be connected to the lower surface  111   e  of the storage chamber  110   e  by ultrasonic fusing, heat sealing, or laser radiation. 
     The sampling assembly  300   e  includes a seat  310   e  and a sampling member  330   e . The seat  310   e  is arranged adjacent to the bottom opening  112   e  and disposed on the lower surface  111   e  of the storage chamber  110   e . The sampling member  330   e  is disposed on the seat  310   e  and extends along a direction away from the lower surface  111   e  of the storage chamber  110   e . A passage  370   e  is formed in the sampling member  330   e . The passage  370   e  is used to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell test sample, or any other bodily fluid. A fluid inlet  371   e  and a fluid outlet  373   e  are formed at two end of the passage  370   e , and fluid can flow through the passage  370   e  via the fluid inlet  371   e  and the fluid outlet  373   e . In the embodiment, the storage chamber  110   e  and the sampling assembly  300   e  are formed integrally by for example, plastic injection molding. Therefore, the storage chamber  110   e  and the sampling assembly  300   e  constitute a single assembly which is served to collect test sample F 2  and hold at least fluid F 1 . However, the storage chamber  110   e  and the sampling assembly  300   e  may be two individual units and made by two different materials such as plastic material and glass. The two units may be connected to each other by a method including screwing or clamping. 
     In the embodiment, a flow path  130   e  is defined in the testing module  1   e . Specifically, an upstream  131   e  of the flow path  130   e  is formed in the storage chamber  110   e , and a downstream  133   e  of the flow path  130   e  is formed in the mixing chamber  150   e . The fluid F 1  and/or the fluid F 1 ′ from the storage chamber  110   e  flows to the mixing chamber  150   e  via the flow path  130   e.    
     Referring to  FIGS. 17-19 , the operation method of testing the test sample F 2  by the testing module  1   e  according to the fifth embodiment of the disclosure is described below. 
     In the beginning, as shown in  FIG. 17 , the fluid F 1  and/or the fluid F 1 ′ is provided in the storage chamber  110   e . Before the connection of the sampling assembly  300   e  and the carrier  100   e , the block structure  200   e  is in a first state, in which the storage chamber  110   e  is sealed by the block structure  200   e  so that the fluid F 1  is held in the storage chamber  110   e  safely. The first state of the block structure  200   e  refers to the membrane (the block structure  200   e ) is intact without breakage. Afterwards, the test sample F 2  is collected in the passage  370   e . The test sample F 2  is kept in the passage  370   b  through capillary force. 
     Afterwards, the storage chamber  110   e  and the sampling assembly  300   e  are transported along a direction indicated by the arrow shown in  FIG. 17  and placed into the accommodating space  123   e  via the top lateral edge  1231   e  of the base  120   e , wherein the sampling member  330   e  directly faces the through hole  107   e , and the block structure  200   e  directly faces the puncturing structures  105   e . It should be noted that during connecting the storage chamber  110   e  and the sampling assembly  300   e  to the carrier  100   e , the puncturing structures  105   e  penetrate the block structure  200   e  so that the block structure  200   e  transforms to a second state, in which the membrane (the block structure  200   e ) is piercingly penetrated. Afterwards, openings are formed on the membrane  200   e . The movement of the storage chamber  110   e  and the sampling assembly  300   e  is stopped as the storage chamber  110   e  abuts against the supporting member  108   e.    
     At this moment, as shown in  FIG. 18 , the fluid F 1  and/or the fluid F 1 ′ flows out of the storage chamber  110   e  via the upstream  131   e . It is noted that since there are depressed portion  1051   e  formed on the puncturing structures  105   e , the fluid F 1  and/or the fluid F 1 ′ from the storage chamber  110   e  can be flow out of the storage chamber  110   e  via the depressed portion  1051   e . Afterwards, the fluid F 1  and/or the fluid F 1 ′ are driven to flow into the mixing chamber  150   e  via the downstream  133   e . Before the fluid F 1  and/or the fluid F 1 ′ flow into the mixing chamber  150   e , a portion of the fluid F 1  and/or the fluid F 1 ′ flows into the mixing chamber  150   e  via the through hole  107   e , and the other portion of the fluid F 1  and/or the fluid F 1 ′ flow into the mixing chamber  150   e  via the passage  370   e  after mixing with the test sample F 2  in the passage  370   e . Specifically, the fluid F 1  and/or the fluid F 1 ′ enter the passage  370   e  via the fluid inlet  371   e  of the passage  370   e  and leaves the passage  370   e  via the fluid outlet  373   e  of the passage  370   e  together with the test sample F 2 . In the embodiment, the viscosity of the fluid F 1  and/or the fluid F 1 ′ are lower than that of the test sample F 2  so as to facilitate the fluid F 1  and/or the fluid F 1 ′ flushing the test sample F 2  out of the passage  370   e . In another embodiment, the viscosity of the fluid F 1  and/or the fluid F 1 ′ are higher than or equal to that of the test sample F 2 , the fluid F 1  and/or the fluid F 1 ′ will enter the passage  370   e  and bring the test sample F 2  to the mixing chamber  150   e . In some embodiments, once the fluid F 1  and/or the fluid F 1 ′ and the test sample F 2  enters the mixing chamber  150   e  and are uniformly mixed to form a mixture F 4 , the reaction between the fluid F 1  and/or the fluid F 1 ′ and the test sample F 2  begins. In some embodiments, if the fluid F 1  is a reactive agent and the fluid F 1 ′ is a diluent, a reaction of the fluid F 1  and the fluid F 1 ′ may or may not begin in the passage  370   e . Last, after the reaction of the fluid F 1  and/or the fluid F 1 ′ and the test sample F 2  is finished a measurement of the reaction result is performed. Therefore, the process of testing the test sample F 2  is completed. 
     Referring to  FIG. 19 , in the fifth embodiment, the operation of driving the fluid F 1  and/or the fluid F 1 ′ to flow into the mixing chamber  150   e  includes placing the testing module  1   e  as a whole on a rotation plate  500   e , wherein the storage chamber  110   e  is closer to a rotation center of the rotation plate  500   e  than the mixing chamber  150   e . Afterwards, the rotation plate  500   e  is rotated about a rotation axis A so as to generate a centrifugal force to drive the fluid F 1  to flow. In another embodiment, the operation of driving the fluid F 1  and/or the fluid F 1 ′ to flow out of the storage chamber  110   e  includes providing a pump to drive the fluid F 1  and/or the fluid F 1 ′ to flow. 
     In the fifth embodiment, while there are two punctuating structures  105   e  are arranged, the number of the punctuating structure  105   e  may be modified according to the number of the storage spaces formed in the storage chamber  110   e , wherein each punctuating structure  105   e  faces one of the storage spaces to enable the fluid or the reactive reagent in the storage space to be released, and the fluid or the reactive reagent flows into the mixing chamber  150   e  via the through hole  170   e  or the passage  370   e.    
     Sixth Embodiment 
       FIG. 20  shows an exploded structural view of a testing module if of the sixth embodiment of the disclosure. In the sixth embodiment, the testing module if includes a carrier  100   f , two storage chambers  110   f , a holder  160   f , a number of block structures  200   f , and a sampling assembly  300   f.    
     The carrier  100   f  includes a base  120   f , an accommodating space  123   f , and a mixing chamber  150   f . The accommodating space  123   f  is formed on an upper surface of the base  120   f  and arranged adjacent to a top lateral edge  1231   f  of the base  120   f . The mixing chamber  150   f  is formed on the upper surface of the base  120   f  and arranged adjacent to the accommodating space  123   f . The accommodating space  123   f  communicates with the mixing chamber  150   f  via a through hole  107   f . A cover (not shown in  FIGS. 20 and 21 ) covers the upper surface of the base  120   f , so as to seal the accommodating space  123   f  and the mixing chamber  150   f.    
     Two storage chambers  110   f  are disposed in the accommodating space  123   f . In the embodiment, each storage chamber  110   f  has a hollow structure. A top opening  114   f  is formed on the upper surface  112   f  of each storage chamber  110   f , and a membrane  180   f  is disposed on the upper surface  112   f  relative to the top opening  114   f  of each storage chamber  110   f . A bottom opening  116   f  is formed on the lower surface  111   f  of each storage chamber  110   f , and a block structure  200   f  is disposed on the lower surface  111   f  relative to the bottom opening  116   f  of each storage chamber  110   f . In the sixth embodiment, the block structures  200   f  are membranes, such as aluminum membranes. The block structures  200   f  may be connected to the lower surface of each storage chamber  110   f  by ultrasonic fusing, heat sealing, or laser radiation. The storage chambers  110   f  may be used to hold the same or different fluid. For example, one of the storage chamber  110   f  holds the fluid F 1 , such as a reactive reagent, and the other storage chamber  110   f  holds the different fluid F 1 ′, such as a diluent. Alternatively, additional storage chambers  110   f  can be added so as to hold different fluids or reactive reagents. In some embodiments, the selection of the liquid in the mixing chamber  150   f  is determined according to the liquid held by the storage chamber  110   f . For example, the mixing chamber  150   f  may hold reactive reagents. Alternatively, there is no liquid in the mixing chamber  150   f.    
     The holder  160   f  includes a first lower surface  161   f  and a second lower surface  163   f , the first lower surface  161   f  connects to the second lower surface  163   f  via the lateral surface  162   f . A number of punctuating structures  165   f  are respectively formed on the first lower surface  161   f  of the holder  160   f  and extend along a direction toward the accommodating space  123   f  and terminate at their respective end portion. In some embodiments, the punctuating structures  165   f  and the holder  160   f  are formed integrally. In some embodiments, the end portion of each punctuating structure  165   f  has a sharp tip. In some embodiments, the extension length of each punctuating structure  165   f  is smaller than the height of the lateral surface  162   f  of the holder  160   f  It is appreciated that the number of the punctuating structures  165   f  should not be limited. The number of the punctuating structures  165   f  corresponds to that of the storage chamber  110   f.    
     The sampling assembly  300   f  includes a seat  310   f  and a sampling member  330   f . The seat  310   f  is disposed on the second lower surface  163   f  of the holder  160   f . The sampling member  330   f  is disposed on the seat  310   f  and extends along a direction away from the second lower surface  163   f  of the holder  160   f . A passage  370   f  is formed in the sampling member  330   f . The passage  370   f  is used to collect the test sample F 2  such as blood, urine, sputum, semen, feces, pus, tissue fluid, bone marrow, cell sample, or any other bodily fluid. A fluid inlet  371   f  and a fluid outlet  373   f  are formed at two end of the passage  370   f , and fluid can flow through the passage  370   f  via the fluid inlet  371   f  and the fluid outlet  373   f.    
     In the embodiment, a flow path  130   f  is defined in the testing module  1   f . Specifically, an upstream  131   f  of the flow path  130   f  is formed in the storage chamber  110   f , and a downstream  133   f  of the flow path  130   f  is formed in the mixing chamber  150   f . The fluid F 1  from the storage chamber  110   f  flows to the mixing chamber  150   f  via the flow path  130   f.    
     Referring to  FIGS. 20-21 , the operation method of testing the test sample F 2  by the testing module if according to the sixth embodiment of the disclosure is described below. 
     In the beginning, as shown in  FIG. 20 , the fluid F 1  and/or the fluid F 1 ′ is provided in the storage chambers  110   f  Before the connection of the sampling assembly  300   f  and the carrier  100   f , the block structures  200   f  are in a first state, in which the storage chambers  110   f  are respectively sealed by the block structures  200   f  so that the fluid F 1  and/or the fluid F 1 ′ is held in the storage chambers  110   e  safely. The first state of the block structure  200   e  refers to the membranes (the block structures  200   f ) are intact without breakage. Afterwards, the test sample F 2  is collected in the passage  370   f  and kept in the passage  370   b  through capillary force. 
     Afterwards, the holder  160   f  and the sampling assembly  300   f  are transported along a direction indicated by the arrow shown in  FIG. 20  and placed into the accommodating space  123   f  via the top lateral edge  1231   f  of the base  120   f , wherein the sampling member  330   f  directly faces the through hole  107   f , and the puncturing structures  105   f  directly face the block structures  165   f  respectively. It should be noted that during the connection of the holder  160   f  and the sampling assembly  300   f  to the carrier  100   f , the puncturing structures  105   f  respectively penetrate the block structures  200   f  so that the block structures  200   f  transform to a second stage, in which each membrane (the block structure  2000  is piercingly penetrated. Afterwards, an opening is formed on the membranes  200   f.    
     At this moment, as shown in  FIG. 21 , the fluid F 1  and/or the fluid F 1 ′ flow out of the storage chambers  110   f  via the upstream  131   f  Afterwards, the fluid F and/or the fluid F 1 ′ are driven to flow into the mixing chamber  150   f  via the downstream  133   f . Before the fluid F 1  and/or the fluid F 1 ′ flow into the mixing chamber  150   f , a portion of the fluid F 1  and/or the fluid F 1 ′ flow into the mixing chamber  150   f  via the through hole  107   f , and the other portion of the fluid F 1  and/or the fluid F 1 ′ flow into the mixing chamber  150   f  via the passage  370   f  after mixing with the test sample F 2  in the passage  370   f . Specifically, the fluid F 1  and/or the fluid F 1 ′ enter the passage  370   f  via the fluid inlet  371   f  of the passage  370   f  and leaves the passage  370   f  via the fluid outlet  373   f  of the passage  370   e  together with the test sample F 2 . In the embodiment, the viscosity of the fluid F 1  and/or the fluid F 1 ′ are lower than that of the test sample F 2  so as to facilitate the fluid F 1  and/or the fluid F 1 ′ flushing the test sample F 2  out of the passage  370   f . In another embodiment, the viscosity of the fluid F 1  and/or the fluid F 1 ′ are higher than or equal to that of the test sample F 2 , the fluid F 1  and/or the fluid F 1 ′ will enter the passage  370   f  and bring the test sample F 2  to the mixing chamber  150   f . Once the fluid F 1  and/or the fluid F 1 ′ and the test sample F 2  enters the mixing chamber  150   f  and are uniformly mixed to form a mixture F 4 , the reaction between the fluid F 1  and/or the fluid F 1 ′ and the test sample F 2  begins. Alternatively, a reaction of the fluid F 1  and the fluid F 1 ′ may begin in the passage  370   f . Last, after the reaction of the fluid F 1  and/or the fluid F 1 ′ and the test sample F 2  is finished, a measurement of the reaction result is performed. Therefore, the process of testing the test sample F 2  is completed. 
     In the sixth embodiment, the operation of driving the fluid F 1  and/or the fluid F 1 ′ to flow into the mixing chamber  150   f  includes placing the testing module if as a whole on a rotation plate, wherein the storage chamber  110   f  is closer to a rotation center of the rotation plate than the mixing chamber  150   f . Afterwards, the rotation plate is rotated about a rotation axis rotate the rotation plate so as to generate a centrifugal force to the fluid F 1  and/or the fluid F 1 ′ are driven to flow. In another embodiment, the operation of driving the fluid F 1  and/or the fluid F 1 ′ to flow out of the storage chamber  110   f  includes providing a pump to drive the fluid F 1  and/or the fluid F 1 ′ to flow. 
     In the sixth embodiment, while there are two punctuating structures  105   f  are arranged, the number of the punctuating structure  105   f  may be modified according to the number of the storage chamber  110   f  wherein each punctuating structure  105   f  faces one of the storage chambers  110   f , to enable the fluid or the reactive reagent in the storage chamber to be released, and the fluid or the reactive reagent flows into the mixing chamber  150   f  via the through hole  170   f  or the passage  370   f.    
     With the design that the fluid flushes the test sample into the mixing chamber, the testing module of the disclosure achieves the functions of liquid transporting, liquid dilution, and liquid mixing. In addition, since the process operations are reduced, the testing efficiency is improved. 
     While the invention has been described by way of example and in terms of preferred embodiment, it is to be understood that the invention is not limited thereto. On the contrary, it is intended to cover various modifications and similar arrangements (as would be apparent to those skilled in the art). Therefore, the scope of the appended claims should be accorded the broadest interpretation so as to encompass all such modifications and similar arrangements.